LOCUS       DACCWK010000001        91634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCWK010000001 DACCWK010000000
VERSION     DACCWK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.001"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(35..108)
                     /locus_tag="IVW65_000001"
     tRNA            complement(35..108)
                     /locus_tag="IVW65_000001"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Ile,seq:cat)
     gene            complement(298..1323)
                     /locus_tag="IVW65_000002"
     CDS             complement(298..1323)
                     /locus_tag="IVW65_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7898939.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            complement(1320..1544)
                     /locus_tag="IVW65_000003"
     CDS             complement(1320..1544)
                     /locus_tag="IVW65_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7898940.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1541..1834)
                     /locus_tag="IVW65_000004"
     CDS             complement(1541..1834)
                     /locus_tag="IVW65_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898941.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            complement(1872..2009)
                     /locus_tag="IVW65_000005"
     CDS             complement(1872..2009)
                     /locus_tag="IVW65_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7898942.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            complement(2011..2457)
                     /locus_tag="IVW65_000006"
     CDS             complement(2011..2457)
                     /locus_tag="IVW65_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898943.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(2620..4746)
                     /locus_tag="IVW65_000007"
     CDS             complement(2620..4746)
                     /locus_tag="IVW65_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898944.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            complement(4770..7061)
                     /locus_tag="IVW65_000008"
     CDS             complement(4770..7061)
                     /locus_tag="IVW65_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898945.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            complement(7071..7808)
                     /locus_tag="IVW65_000009"
     CDS             complement(7071..7808)
                     /locus_tag="IVW65_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7898946.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            complement(7859..11290)
                     /locus_tag="IVW65_000010"
     CDS             complement(7859..11290)
                     /locus_tag="IVW65_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7898947.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            complement(11307..11489)
                     /locus_tag="IVW65_000011"
     CDS             complement(11307..11489)
                     /locus_tag="IVW65_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898948.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            complement(11492..11854)
                     /locus_tag="IVW65_000012"
     CDS             complement(11492..11854)
                     /locus_tag="IVW65_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898949.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            complement(11874..12482)
                     /locus_tag="IVW65_000013"
     CDS             complement(11874..12482)
                     /locus_tag="IVW65_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7898950.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            complement(12494..12898)
                     /locus_tag="IVW65_000014"
     CDS             complement(12494..12898)
                     /locus_tag="IVW65_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898951.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(12891..13292)
                     /locus_tag="IVW65_000015"
     CDS             complement(12891..13292)
                     /locus_tag="IVW65_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898952.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(13282..13635)
                     /locus_tag="IVW65_000016"
     CDS             complement(13282..13635)
                     /locus_tag="IVW65_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7898953.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(13625..13936)
                     /locus_tag="IVW65_000017"
     CDS             complement(13625..13936)
                     /locus_tag="IVW65_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898954.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(13933..14808)
                     /locus_tag="IVW65_000018"
     CDS             complement(13933..14808)
                     /locus_tag="IVW65_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898955.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(14818..15978)
                     /locus_tag="IVW65_000019"
     CDS             complement(14818..15978)
                     /locus_tag="IVW65_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7898956.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(15978..16664)
                     /locus_tag="IVW65_000020"
     CDS             complement(15978..16664)
                     /locus_tag="IVW65_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7898957.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(16627..17805)
                     /locus_tag="IVW65_000021"
     CDS             complement(16627..17805)
                     /locus_tag="IVW65_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7898958.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(17825..19519)
                     /locus_tag="IVW65_000022"
     CDS             complement(17825..19519)
                     /locus_tag="IVW65_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7898959.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(19497..19811)
                     /locus_tag="IVW65_000023"
     CDS             complement(19497..19811)
                     /locus_tag="IVW65_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7898960.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(19914..20195)
                     /locus_tag="IVW65_000024"
     CDS             complement(19914..20195)
                     /locus_tag="IVW65_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898961.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(20200..20544)
                     /locus_tag="IVW65_000025"
     CDS             complement(20200..20544)
                     /locus_tag="IVW65_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7898962.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(20570..20737)
                     /locus_tag="IVW65_000026"
     CDS             complement(20570..20737)
                     /locus_tag="IVW65_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7898963.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            complement(20933..21139)
                     /locus_tag="IVW65_000027"
     CDS             complement(20933..21139)
                     /locus_tag="IVW65_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898964.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            21593..21868
                     /locus_tag="IVW65_000028"
     CDS             21593..21868
                     /locus_tag="IVW65_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898965.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(22326..22739)
                     /locus_tag="IVW65_000029"
     CDS             complement(22326..22739)
                     /locus_tag="IVW65_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7898966.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(22816..23112)
                     /locus_tag="IVW65_000030"
     CDS             complement(22816..23112)
                     /locus_tag="IVW65_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898967.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(23109..23666)
                     /locus_tag="IVW65_000031"
     CDS             complement(23109..23666)
                     /locus_tag="IVW65_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP7898968.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(23663..24082)
                     /locus_tag="IVW65_000032"
     CDS             complement(23663..24082)
                     /locus_tag="IVW65_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP7898969.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(24079..24324)
                     /locus_tag="IVW65_000033"
     CDS             complement(24079..24324)
                     /locus_tag="IVW65_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898970.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(24284..24640)
                     /locus_tag="IVW65_000034"
     CDS             complement(24284..24640)
                     /locus_tag="IVW65_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7898971.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(24640..24945)
                     /locus_tag="IVW65_000035"
     CDS             complement(24640..24945)
                     /locus_tag="IVW65_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898972.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(24942..25103)
                     /locus_tag="IVW65_000036"
     CDS             complement(24942..25103)
                     /locus_tag="IVW65_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7898973.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(25100..25402)
                     /locus_tag="IVW65_000037"
     CDS             complement(25100..25402)
                     /locus_tag="IVW65_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898974.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(25564..25833)
                     /locus_tag="IVW65_000038"
     CDS             complement(25564..25833)
                     /locus_tag="IVW65_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898975.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(25845..26594)
                     /locus_tag="IVW65_000039"
     CDS             complement(25845..26594)
                     /locus_tag="IVW65_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7898976.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(26597..27283)
                     /locus_tag="IVW65_000040"
     CDS             complement(26597..27283)
                     /locus_tag="IVW65_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898977.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(27289..27960)
                     /locus_tag="IVW65_000041"
     CDS             complement(27289..27960)
                     /locus_tag="IVW65_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7898978.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(27953..28294)
                     /locus_tag="IVW65_000042"
     CDS             complement(27953..28294)
                     /locus_tag="IVW65_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898979.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(28472..29170)
                     /locus_tag="IVW65_000043"
     CDS             complement(28472..29170)
                     /locus_tag="IVW65_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7898980.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            29257..29727
                     /locus_tag="IVW65_000044"
     CDS             29257..29727
                     /locus_tag="IVW65_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898981.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(29770..29949)
                     /locus_tag="IVW65_000045"
     CDS             complement(29770..29949)
                     /locus_tag="IVW65_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898982.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(29936..30274)
                     /locus_tag="IVW65_000046"
     CDS             complement(29936..30274)
                     /locus_tag="IVW65_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7898983.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(30290..30493)
                     /locus_tag="IVW65_000047"
     CDS             complement(30290..30493)
                     /locus_tag="IVW65_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7898984.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(30508..31284)
                     /locus_tag="IVW65_000048"
     CDS             complement(30508..31284)
                     /locus_tag="IVW65_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7898985.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            31583..31903
                     /locus_tag="IVW65_000049"
     CDS             31583..31903
                     /locus_tag="IVW65_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7898986.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            31921..32352
                     /locus_tag="IVW65_000050"
     CDS             31921..32352
                     /locus_tag="IVW65_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7898987.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            32482..32943
                     /locus_tag="IVW65_000051"
     CDS             32482..32943
                     /locus_tag="IVW65_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP7898988.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            32977..33612
                     /locus_tag="IVW65_000052"
     CDS             32977..33612
                     /locus_tag="IVW65_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP7898989.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            33725..34360
                     /locus_tag="IVW65_000053"
     CDS             33725..34360
                     /locus_tag="IVW65_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898990.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            34659..35798
                     /locus_tag="IVW65_000054"
     CDS             34659..35798
                     /locus_tag="IVW65_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7898991.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(35867..35940)
                     /locus_tag="IVW65_000055"
     tRNA            complement(35867..35940)
                     /locus_tag="IVW65_000055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35904..35906),aa:Arg,seq:cct)
     gene            complement(36329..37509)
                     /locus_tag="IVW65_000056"
                     /pseudo
     CDS             complement(36329..37509)
                     /locus_tag="IVW65_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(37632..38045)
                     /locus_tag="IVW65_000057"
     CDS             complement(37632..38045)
                     /locus_tag="IVW65_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7898992.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            complement(38045..38227)
                     /locus_tag="IVW65_000058"
     CDS             complement(38045..38227)
                     /locus_tag="IVW65_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7898993.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            complement(38258..38479)
                     /locus_tag="IVW65_000059"
     CDS             complement(38258..38479)
                     /locus_tag="IVW65_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7898994.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            complement(38547..40007)
                     /locus_tag="IVW65_000060"
     CDS             complement(38547..40007)
                     /locus_tag="IVW65_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7898995.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            complement(40115..40435)
                     /locus_tag="IVW65_000061"
     CDS             complement(40115..40435)
                     /locus_tag="IVW65_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7898996.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            complement(40465..40782)
                     /locus_tag="IVW65_000062"
     CDS             complement(40465..40782)
                     /locus_tag="IVW65_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7898997.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            complement(40934..43636)
                     /locus_tag="IVW65_000063"
     CDS             complement(40934..43636)
                     /locus_tag="IVW65_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7898998.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            complement(43699..44976)
                     /locus_tag="IVW65_000064"
     CDS             complement(43699..44976)
                     /locus_tag="IVW65_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7898999.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            complement(44976..45989)
                     /locus_tag="IVW65_000065"
     CDS             complement(44976..45989)
                     /locus_tag="IVW65_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7899000.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            complement(46005..46733)
                     /gene="sfsA"
                     /locus_tag="IVW65_000066"
     CDS             complement(46005..46733)
                     /gene="sfsA"
                     /locus_tag="IVW65_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7899001.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            46910..47239
                     /locus_tag="IVW65_000067"
     CDS             46910..47239
                     /locus_tag="IVW65_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7899002.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            47423..48070
                     /locus_tag="IVW65_000068"
     CDS             47423..48070
                     /locus_tag="IVW65_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7899003.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            48085..49611
                     /locus_tag="IVW65_000069"
     CDS             48085..49611
                     /locus_tag="IVW65_000069"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7899004.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(49660..52458)
                     /gene="ileS"
                     /locus_tag="IVW65_000070"
     CDS             complement(49660..52458)
                     /gene="ileS"
                     /locus_tag="IVW65_000070"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7899005.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(52720..53427)
                     /locus_tag="IVW65_000071"
     CDS             complement(52720..53427)
                     /locus_tag="IVW65_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7899006.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(53448..54230)
                     /locus_tag="IVW65_000072"
     CDS             complement(53448..54230)
                     /locus_tag="IVW65_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7899007.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(54279..54566)
                     /locus_tag="IVW65_000073"
     CDS             complement(54279..54566)
                     /locus_tag="IVW65_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7899008.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(54581..55180)
                     /locus_tag="IVW65_000074"
     CDS             complement(54581..55180)
                     /locus_tag="IVW65_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7899009.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(55194..55871)
                     /locus_tag="IVW65_000075"
     CDS             complement(55194..55871)
                     /locus_tag="IVW65_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7899010.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(55888..57129)
                     /gene="ftsZ"
                     /locus_tag="IVW65_000076"
     CDS             complement(55888..57129)
                     /gene="ftsZ"
                     /locus_tag="IVW65_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7899011.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(57152..58477)
                     /gene="ftsA"
                     /locus_tag="IVW65_000077"
     CDS             complement(57152..58477)
                     /gene="ftsA"
                     /locus_tag="IVW65_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7899012.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(58633..59853)
                     /locus_tag="IVW65_000078"
     CDS             complement(58633..59853)
                     /locus_tag="IVW65_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7899013.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(59868..60956)
                     /gene="murG"
                     /locus_tag="IVW65_000079"
     CDS             complement(59868..60956)
                     /gene="murG"
                     /locus_tag="IVW65_000079"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7899014.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(60982..62343)
                     /locus_tag="IVW65_000080"
     CDS             complement(60982..62343)
                     /locus_tag="IVW65_000080"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7899015.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(62357..63319)
                     /locus_tag="IVW65_000081"
     CDS             complement(62357..63319)
                     /locus_tag="IVW65_000081"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7899016.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(63345..65537)
                     /locus_tag="IVW65_000082"
     CDS             complement(63345..65537)
                     /locus_tag="IVW65_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7899017.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(65538..65939)
                     /gene="ftsL"
                     /locus_tag="IVW65_000083"
     CDS             complement(65538..65939)
                     /gene="ftsL"
                     /locus_tag="IVW65_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7899018.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(65944..66903)
                     /gene="rsmH"
                     /locus_tag="IVW65_000084"
     CDS             complement(65944..66903)
                     /gene="rsmH"
                     /locus_tag="IVW65_000084"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7899019.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(66924..67355)
                     /gene="mraZ"
                     /locus_tag="IVW65_000085"
     CDS             complement(66924..67355)
                     /gene="mraZ"
                     /locus_tag="IVW65_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7899020.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(67525..67893)
                     /locus_tag="IVW65_000086"
     CDS             complement(67525..67893)
                     /locus_tag="IVW65_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7899021.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            68064..69020
                     /locus_tag="IVW65_000087"
     CDS             68064..69020
                     /locus_tag="IVW65_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7899022.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            69093..69209
                     /locus_tag="IVW65_000088"
     CDS             69093..69209
                     /locus_tag="IVW65_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7899023.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(69264..70946)
                     /gene="recN"
                     /locus_tag="IVW65_000089"
     CDS             complement(69264..70946)
                     /gene="recN"
                     /locus_tag="IVW65_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7899024.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(70964..71413)
                     /locus_tag="IVW65_000090"
     CDS             complement(70964..71413)
                     /locus_tag="IVW65_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7899025.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(71520..72338)
                     /locus_tag="IVW65_000091"
     CDS             complement(71520..72338)
                     /locus_tag="IVW65_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7899026.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(72347..73237)
                     /locus_tag="IVW65_000092"
     CDS             complement(72347..73237)
                     /locus_tag="IVW65_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7899027.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(73239..73463)
                     /locus_tag="IVW65_000093"
     CDS             complement(73239..73463)
                     /locus_tag="IVW65_000093"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7899028.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(73468..74805)
                     /locus_tag="IVW65_000094"
     CDS             complement(73468..74805)
                     /locus_tag="IVW65_000094"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7899029.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(74806..75660)
                     /locus_tag="IVW65_000095"
     CDS             complement(74806..75660)
                     /locus_tag="IVW65_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7899030.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(75757..76206)
                     /gene="nusB"
                     /locus_tag="IVW65_000096"
     CDS             complement(75757..76206)
                     /gene="nusB"
                     /locus_tag="IVW65_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7899031.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(76199..76624)
                     /locus_tag="IVW65_000097"
     CDS             complement(76199..76624)
                     /locus_tag="IVW65_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7899032.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(76870..77934)
                     /locus_tag="IVW65_000098"
     CDS             complement(76870..77934)
                     /locus_tag="IVW65_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7899033.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(78106..78351)
                     /locus_tag="IVW65_000099"
     CDS             complement(78106..78351)
                     /locus_tag="IVW65_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7899034.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(78490..78864)
                     /locus_tag="IVW65_000100"
     CDS             complement(78490..78864)
                     /locus_tag="IVW65_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7899035.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(78947..79861)
                     /locus_tag="IVW65_000101"
     CDS             complement(78947..79861)
                     /locus_tag="IVW65_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7899036.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(79882..80685)
                     /locus_tag="IVW65_000102"
     CDS             complement(79882..80685)
                     /locus_tag="IVW65_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7899037.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(80718..81707)
                     /gene="manX"
                     /locus_tag="IVW65_000103"
     CDS             complement(80718..81707)
                     /gene="manX"
                     /locus_tag="IVW65_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7899038.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(81810..82301)
                     /locus_tag="IVW65_000104"
     CDS             complement(81810..82301)
                     /locus_tag="IVW65_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7899039.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(82492..85326)
                     /locus_tag="IVW65_000105"
     CDS             complement(82492..85326)
                     /locus_tag="IVW65_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7899040.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(85733..86026)
                     /gene="rpmA"
                     /locus_tag="IVW65_000106"
     CDS             complement(85733..86026)
                     /gene="rpmA"
                     /locus_tag="IVW65_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7899041.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(86039..86383)
                     /locus_tag="IVW65_000107"
     CDS             complement(86039..86383)
                     /locus_tag="IVW65_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7899042.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(86410..86718)
                     /gene="rplU"
                     /locus_tag="IVW65_000108"
     CDS             complement(86410..86718)
                     /gene="rplU"
                     /locus_tag="IVW65_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7899043.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            87000..87641
                     /locus_tag="IVW65_000109"
     CDS             87000..87641
                     /locus_tag="IVW65_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7899044.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(87703..89334)
                     /locus_tag="IVW65_000110"
     CDS             complement(87703..89334)
                     /locus_tag="IVW65_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7899045.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(89457..89816)
                     /locus_tag="IVW65_000111"
     CDS             complement(89457..89816)
                     /locus_tag="IVW65_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7899046.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(89975..90048)
                     /locus_tag="IVW65_000112"
     tRNA            complement(89975..90048)
                     /locus_tag="IVW65_000112"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90012..90014),aa:Arg,seq:tct)
     gene            complement(90357..91040)
                     /locus_tag="IVW65_000113"
     CDS             complement(90357..91040)
                     /locus_tag="IVW65_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7899047.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(91168..91608)
                     /locus_tag="IVW65_000114"
     CDS             complement(91168..91608)
                     /locus_tag="IVW65_000114"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7899048.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT        26427 a        19291 c        14948 g        30968 t
ORIGIN      
        1 ttctaacaca gaacgattca aatggctacg ttaatgtacc ctgtaggact cgaacctacg
       61 accggacggt tatgagccgt ctgctctgac caactgagct aagggtactg gttgttgcca
      121 cataaagcca taaacaatca accagtagaa tgtgtggcaa caaacctgtt atcgcatagc
      181 ttggagtgtg actatttatg ggtgatagtg aagatatgcg ataacatcac tattttatcg
      241 aataattttt atagttgtca atatagttat gtactgctcc tcaacgagga gctattttta
      301 tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac cgcctttagt
      361 gtttccctct ggatcattcg ttgcccgcat cattacatag actttcttac taggaaaatt
      421 acgcatattg aaagatacat gataaccaac atttccagaa gtattataag cttggtttac
      481 atctggtcta taaattccat cagctcttac tcgagctaat tctttcccag tattgtagtc
      541 cataatgaaa atatactcgt atttatagtt agcaatgtgc catccagcca catgcaagtt
      601 tgcgttttcg atttccccaa actgatcaat gtgggcgtga tttgttccat ctgtcagcgt
      661 aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag aagttgttgg
      721 attttcatcg gtaaatccat gagctaaatc ataagcaagc ttttctttgc taactcccat
      781 ttgcgataag taaccatatg gatctgtgtg gttcccccaa acataatttg tcacccacaa
      841 atgagaaatg attccttttg taaataaaga agttccttga tcaagagtca atggaattcc
      901 atattttttt gcactatctc ttgtatattc aatataagct cgatagtttt tttcaaacaa
      961 tgctttatca tatgtgcgct gtaattcaat ctgtacaggc gcataaggat tagcgttacc
     1021 agctccccac gaaacatatc cttgctcacc cacacggtaa acaatcccac cgtcaccaat
     1081 aacatctgta gtataaggat tgcttccgtt ataattattt ttcatgttgg cggctacgtt
     1141 tcttgctggt gcatctattc cagtttcgtg caaaataatt ttgttaggaa ttgctaatct
     1201 ggagtctcct tgattcggcg ctaaattata ctcgtcatta atagtataag caaacgtatt
     1261 aatgggtaat aaaaaaagag ccgttaacag gctcatcgca gtaatagtaa ttttcttttt
     1321 catttgtttc ctccttcttc gctttcagcc gagaacattt tgtaggttcg atttgataca
     1381 cccaacacac tccctaaaaa cgcgccaaaa ccagtaatga tgacaacaca gatatctgtg
     1441 tactgccaat tgagcgcttt accaactaac cccacgaaag tagctagtgc gggaataatt
     1501 accagtgcga accattttag tacttcgaac gttttattat tcattttctt ctctccctaa
     1561 ataaagtttt aatttgttgc gtgtgttcta ccaatttttc tgcatgtgta tctaatcttt
     1621 catcgtgttt ctttagttct tcatgaatca tcaatcgatc tgatttgctc gattctaaat
     1681 ctttagtcag caaatctaaa ttgtgactta cttttgaaag agtctcagta atcttcgaga
     1741 aagatgcagt aattggtttt attactaata aaatcaaaga aacgatagcg gttattgatc
     1801 ctgctatcgc tccccattcc cctaaattaa tcatgtgaca actccttgaa tcaaaataaa
     1861 aagcacatca attaagatgc gctctcttct ttgctaatga ttttatctgc tttttcttca
     1921 gtaatgcaca acggaacgaa aaccattact tgttcgttag tgaaacagcc ccaatcatac
     1981 atcattttca catcgctaaa actaaacata ctactcacct ccctttgaag ctggatttag
     2041 ttgctcttta atttctgaaa tgtctttgct attttgtaac gaagcaagca tcatttttga
     2101 attgatttgt gctaaactat ccgctttttc tttcaatgca gtattttcct gtttaattgc
     2161 tacatcgctt agcatgagtt tggcattgat ctgttttaaa tcgccgttct cattttctaa
     2221 cgactcatac attgctttga gattgtttaa atcgttgtga tctagtgcgt tcgctaaaat
     2281 aatccattga ttcaatttag gatcaaacat ttgatcagcg attgttaacg gttcgccatc
     2341 agcacgaatt ccttcaagcg gtggctgatc tgtgtaagga acggatacaa gcatgtcgtc
     2401 caatactttt cctgcgtact ctccgccagt acgtccatat ttccaaatgt ttttcattta
     2461 tttccctccc agtaattgaa tttcggtttc caatttggaa tcggtggttc gacttctgtg
     2521 cattcttccg gtaaatgttc ttcatcattc acaatgattt gctcgaatcc gtaaggttca
     2581 attggtctat atgctgcctt catatcgatt cacgcttttc taaatcgtgt aagtaattgc
     2641 aaaggtataa tccgatccat aacttgagtt tcttcgccat ttaatggctc catctgcacc
     2701 aatagataac tgagcactgt tcaaagtaga acggtctatc gagccaacca gttgctcaaa
     2761 actaattggt ggccgatagc cttctggaat tgttagtatc gttgaatcat ttccaccact
     2821 gctttttccg tttaaagcca caaaatatat agaaactgtt tttccttcac gataaagctt
     2881 tgctgatccg gtattcccgt ttgtaactgt taatgtggta gtagctgtat cattaatgcg
     2941 ttcatcgatc ttattgtcta attcatctat agcagtcgca ttagcattcg cttttgtttg
     3001 agcatcctta gctgtggtgt ctacttcatt aattgaagca gtcaactgcg aattaatctc
     3061 cgatactttc ccatcggtat aattgtttgc tttaccagta atttcagaaa ttttaatatc
     3121 tgtggccaaa ttatcttcga catattctgg tgctagatcc caaacataat cttttggatt
     3181 gtttgaatca cgcataccag taccacgata tttatactca ctaatattcg gggttcgagt
     3241 attgcctttt tctagtttga gccactcaat ttgaacagct ccttttgtag ctgacgggta
     3301 ttggtatatt tccagataat tagttacccc actatctata tgttgcttag ttacagtaaa
     3361 agttaactcc catgtattag ctaacccctc agtaggcttc atgtctccta ctttaacgcg
     3421 gccagcagca atatacacgg caaacgattg aactgttagt ttagttgctt tcattgatat
     3481 ggtatatgtt tggtcagcga tatagttttc tgtattgcgt ctaccataaa ccatatattc
     3541 actagttttg tgtggaaaga caactgtagg gtcagcaata ttctcgccta aaggaacttt
     3601 acctaaccag taagggccat ctagtaagtt agggtagtgt gcttttaact taacgtcata
     3661 gctgacacta accgttcctt tatattcact attaagtgag aatatttcaa cataacagtt
     3721 tgttatgcta gttacattat cttttgttat accagtaaat gtatgagtga ccattttatc
     3781 gcgttctaca tttgctggta ttaatgcatt aatgggcagt tctataccac cttgttcatt
     3841 taaatatcta agcctaaccc ctatagtatt ggtctctcca gtccaatctg aaccgacact
     3901 catagtaacg cttaatgtgt atgtcgtaga tggtcgtaaa gtggttataa gatacctatt
     3961 attagcttcc gctgtacacg gaatacctaa gccaagcgat aatatgtcgc cggaattagc
     4021 tgttctagtt ttagtatgag ttataaccct tttaacacta tctttaactg tatgtccatt
     4081 acctaacaaa ggtccaaccc aaggttctgt aatatatggc attaaattcg ggttctccga
     4141 ataatcatag tccccgaagt cgatgctgtt actgtacatc acttgtaagt tacctaactt
     4201 agaaatttct tctttcagag catctaactt gtcttgtagc gttttagctt gaccagttaa
     4261 atcagtaatc tgttgattta agctatccac tctacctttg atttcagcca taaaagcatc
     4321 aaaagtttcg ttgtactttc gaatcaactc ttctaattgc gaaacatatt catcggcttg
     4381 gccttgcgaa atgtcagaca ctcctagtga gaaaaaaatg atatcttgcg ttgttaaaat
     4441 ttgattgtct tttctatatt ctacgtagca gtgtttataa tatcctgctt cactcataaa
     4501 tgtgccatca agagaaaacg tgacttcttc actagttaca ctagttgcaa cattatctac
     4561 gtaacggtta gatggtgttg ttccttttaa agtaaatgtt ccgccactcg tatccatctg
     4621 caagccattt agaaacggtt taaccgtcac cgtaatccct ttatcacctt gacgagccat
     4681 aatagctttg gtgtagttta attctttgct gaaatctaaa gccaaattat ataaactgct
     4741 agccatttat atacctcctt gtcttcgttt taaaaacgtt tttggtcaag cactgtgcta
     4801 tcatatgctg tatcctcttt taatctaata tcttcatacc ctagacggtg tgccactaaa
     4861 ttccatctaa ctaatacgtt tggcttacta gtttcaatga tgaaatggtc aatatcttca
     4921 tgagtaacag cacacaaaac tagttctgta ggtgtcacat gtgtcatata ccgacttaga
     4981 tttactgttt cagcaaacat ggggtcaata tcaacacgaa ctttaccatt gtcacctgta
     5041 acggcttccc cataatcagc gaaataatat tctggagttt cataagcgtt caatagtcgt
     5101 tgtccataat gttctgttgg tacagttgag tttttagtac ctctaacagt aaaatcttta
     5161 tatacttgta ccgttgattg ttcaaaccta gcaagtttcc catcttccca tgaaccaaaa
     5221 aaacaacctg gtaacgttag cataccatca ctagtaaatt tcatagtcct accagctacc
     5281 ttaaattccc atgagttacc cgcactacca ttaatgctta aagaactacc gtcgccagaa
     5341 gttacataac tagcattgct atacctgaaa ttgggcgcac caaaagatag aaacggtctg
     5401 ttattaccat tatcccacgt actaaaaacc aagttaccct gtggatttct aatcatgaaa
     5461 ccaccaccag ttttcatggt gtatgataca ataccggcat cagcacttac ataatcacgt
     5521 gcttctagct ccataatatc tttgttaact ttttttgagt accaagtcat tttgccatta
     5581 gcaatacttg ttctataatc agcaccatca ctaattaatg tagtacctct aatagtaatt
     5641 cctactattt caccagccgt aataaacgag gcattgaatc cgccatctaa cgtccatgcc
     5701 gtttcatatg ttccattaat gccagtttta gaaaaaccaa taccagcatt gttgatttgt
     5761 aaaacattcc ttgcggtatt cttatctggt gtgtccataa tcaaaatacg actaggcgct
     5821 tctttaggat ctaataaaac ataaccacca ttttgaccag taatcatatc agtttgatga
     5881 tctacaatat cattgagtaa atcactgatt tcgccaccgt ttttcaattg atcaatggca
     5941 tcattaatca aattgctgac attattctct gtgttttcta agaagtttgt tttgacgttt
     6001 cctacaacta atttatcgta tgaattggtt agaacattaa acgtatattc cacaattctc
     6061 gctgacatat tcacttttaa ctgtggatga tacacatcta ctccgtcacc catcgaaact
     6121 ttttctagat caacaaattt ttcatagcct ctttgatgcc tcaatggtac taattcaatc
     6181 gaaccactca cttgtggttt ttgtttatct atgtttgttt tcaaccagtc tttagcagct
     6241 tcccttaatg tggctacatc agtcgctttg tctttaaaat caacaaaaga aacatatcca
     6301 gcaggataat catccacgta atccgtgaaa ataacttctt ctggtagagt gatctcgtct
     6361 tctccttctg aagagctgct aatgaatgga taaactccaa ctaaaacact ttgagcatca
     6421 atctctaagt caagaccagt taagttttta gtataaatcg ctttgatttt atgatccgtg
     6481 cctagccttt tttcatgacg taatgtgtta ttatctttta gaaattcccc atgaaatcga
     6541 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
     6601 acattagcaa gcgtatctac taatgacgag aaagaaaact gtgaaggaat aactggtttc
     6661 gctaaagttt ttgcgttttg ccatgcctga ctagcagtga tcttttctgt tccactgtca
     6721 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
     6781 gtattgccta ttacagaagt ctttgcataa taaatccgta gacgctgttt tgctcgattt
     6841 tcatctacat aacactgaat aatacgtcct tctacaatca aatctgcatt agttccgctt
     6901 attggataag taccctgaaa tatctcggct ccgtttaatt tattgctaac agtagctgtt
     6961 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
     7021 gcgtcgtttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
     7081 ccattacgat aaatttgtat tttgctcaaa ccagtacaat taaaataatt aatatccact
     7141 tgcaatgtcg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
     7201 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacgtcagt tcctactact
     7261 aagccttcaa aactaaacac ataatcattt aagatgaact ggcatgaagt agctgaagga
     7321 gtgatgataa tctttggaaa actttcttct aaactattat tcagcaagtt aaatgactgt
     7381 ggtttatcta cggttatagg tacatcttct tgaactcttg cgaatggttt cgcagtaata
     7441 tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat atcgatagtc
     7501 tggataacat aatagacgtt gggatcatca gagaattcta atttctttgc ataatttaac
     7561 caatgacgca tgatataaaa cgattgcttg aacgcttgat ggtcttccac atcctctaaa
     7621 tagttatagt gcaatgtaaa cgacatgtct tcaaacgagt aatcttgtac taagccacct
     7681 aaccttccta aaacagaagt ttcaactctc tgtctttttg gagaaggtat ggttggtctt
     7741 tcagctaaag ccaatttatg caaataatca ggaaatccat cgattataga atgtatacaa
     7801 tcagtcattt tttcacatcc tttttaatac taaaaaaaca ggagaaatac tctcctgttt
     7861 aacgccatgc cgaagcatta tcattttgaa tttttgtaat gctatcaatg atttgttgag
     7921 ttgtttgctt catagtaacc tcatctgcgt taccatcaat tgtgaaattg aattcgtagt
     7981 tattcacagg ttgaatcgtt tgtgccctag atgaaactga tgtgctactc aagatacgat
     8041 caccaatttc ttgcaaaaca gatcttttca aaggtaaaac tgcttcaggt cctgcttcac
     8101 cgacaccgtt cattccacct agtaaagttg gtttagtaaa gatacctcct ttagcatgcc
     8161 attttacacg caaatggggg atttgacctt ttagcgggtt aaagctgcct tccatgataa
     8221 attccggtaa cggaatatgt ggtatagaaa tattcaaatt atcaaaaatg ccactgattt
     8281 tgtctctaat ccaatcaatt ggagcgctaa cagtcttttt gatgccttcc catatgttag
     8341 caattgtact tttaacatta ttgaatatgt cggaaacaat acctgttaga ttggaccaac
     8401 cgcttgaaat tgcatttttt ccatcgttta ctttagagct aatagtgctt gtaattccat
     8461 tccaaagatt caaagcagta tttttgatac cgttccaaat tccgctaatc cacgaagata
     8521 tactattcca aacactttga atggcacttt tagctgcgtt tatagcattg cttatactac
     8581 tagtcacact attccagata tttgatgctg tagagctgat tgaattccaa attccaccta
     8641 accaactaga tacagttgac caaatatttt gaattactgt agcagctgct tgtaccaagc
     8701 tagtgattgt attcttgata ctgttccaaa tactagaagc tgttgcacta attgaattcc
     8761 aaatatttga agccgtagta ctaatagctg tccatatacc attccaccat gccactactg
     8821 gatcaaatat agtatggaat gtagttacaa tcccattcca agcgatactt atccattgtg
     8881 tcatagtatc ccaagtattt ttaaggaaat cagaaatagg tgtccaaaca gcttgccaag
     8941 ctgcgcccaa taactgtcca gctacatcaa aaatacccac gataatatta ataccagctt
     9001 gaatcaatga cgttattaat gtccatggta tttgaacaat tcctacaatg tctgcccaaa
     9061 taatcgacca tacttctttg actccgttcc aaatatttga aacccaatca acgaatgttt
     9121 gccaagtttc ttggactcct tgccagatgt tggaagctcc ttcaactaat ccgctccata
     9181 gctctccaaa ccaatcagaa actccttgcc aaatatcttg aacccaatct acaaatccag
     9241 accaggtttc tttaacccca tcccaaactg atgaggctcc gtcttttata ctttcccaag
     9301 tatcacccaa ccaatcagta aattttttcc ataaaccact aaaccagtca gtaattgcac
     9361 cccagttctt aattgccaca ataacgcctg ctataacagc tataattcct ccaattataa
     9421 gcatcatcgg accaaataaa tatgacacgg ctagtattgc cggtaacagt atgccaaatg
     9481 ctgctgtaag accacctata gcaacaatga aatcctgtac tggttgtgga agattattaa
     9541 acgcatcagc catctttcct agaaaatcaa ttactggttc gagtgcatct atgattgtgt
     9601 tgcctatagg agctaatgaa tcctttaatt cagctatttt cccgttcaac tcttgcaacg
     9661 gagtagtaga atcttcattc attttttgtg cagatccact aacatcatca aatgtatggt
     9721 taacatcagt taaagattgg acaactttca tcgcattatc ttcgccaagc gcagaccaaa
     9781 ttgtcgaagc tttatttaat tggtcgtatt gaccatccat attgctaaaa tcttgaatca
     9841 tggaattaat aacgtctttt tgtgttcctc cgccattttt ccactcttca aaagcttttc
     9901 tagtactttc actaaacata tccatgtttt gctcaaatcg accatctgtt aacgatattc
     9961 ccatttcctt aactaagtca ttgactttat caaggttata agcacccgca tctaaaccat
    10021 tttgaagcat tccgaacgtt tcatcagctg aatatcccat ttgactccat aattggctat
    10081 attctgccat attgtcgcct aattcgtgcg ttttatctaa accgttttga gtacccgaaa
    10141 ccattaaatc cattgcatct tgagcactca agccgaaatt gaccattaag ccatttacac
    10201 cacgtaacgt ttcatccata tcagcgccca tggtgttttc taggaccata gcttgttccg
    10261 tgatattttg taaatcttga ttatttaaat ctcctaaatt acgctttacc aaaatcaatg
    10321 catctgtgga ctgatctaac gattctccaa aacctttata ataaatgtct ctggctacat
    10381 tcgttaattc ttcagcctct tgtttagaca aaccaaaatt agcttgtatc ttactctggg
    10441 aactacctac actgttagca gagtccactg cttgtttccc taattctgta agcttatcgc
    10501 caatgtcgct taaaacgtca gaagcttcca ttaaattatt catatctatt ttgcttccga
    10561 tatcgtccaa gttagttgta tctacatttt tagcagcttg tcctaactct tcaaattcac
    10621 gttcagcatc attaagcttc gcttccatct gcattgcttc tgtggatgta gcgccaaatt
    10681 cagactgtgt agcttctaac tgtcgtctta ggatatctat cgttttctcc gcattttcag
    10741 attgttgaga aacatattct tgggctttcg ctaatttctc ggcttcagaa gctgattgac
    10801 cagcagttgc ttgccatttt ttgtattcgg attcaatcag agaagcacta gcttgaacat
    10861 ttttttgttc gctatccaac tgttgcattg tagactcgta cgtctgtatt tcgccttttg
    10921 cttgagctag tgcattactc gttttatcaa tttcgtttga caaacgttgt tgcgctgttt
    10981 gttgattaat cagttctctt tcaagtttct taacttcggt ggaattttct ccataatatt
    11041 ttttcgcatt ggctaaacgt tggctagtta cttcaacttt ttgactttgt aattcatact
    11101 gcttttctaa agaagataat ttacttccta acttgtctga ttcagaacca gtctgttgta
    11161 attgagcttg ttctagtttt aattctgctc tatttttagt taattcagca ctgatttctt
    11221 ttaacgtaga tttcaatccg tcatcgttag ctatgaaagt tacttctgct tctgttctct
    11281 ttttagccat tttttacctc ctttctttag tttttctggg attggtttat tgcatagttt
    11341 ttccatcctt cataagcact cttgttgtaa gccatttgca aaatgtcatc taaacagata
    11401 tcgcttaaaa ccaaatctga aggcatagaa aaaacgtcgg tcaacatcga atagacatcg
    11461 acccacgttt caactaagag ctttggaatt tttacttttg aagctttttt tccttatttg
    11521 ctttttcaaa ttctttttga taagcatcgc gtgcttgttt gaacatcatc aattgataaa
    11581 tatagctggc agtagccata tcaaaatccc atttatcgat aaattcatcg aatgaaatat
    11641 aatcagtcat gttcgcttgg cggtaagcaa tatacacagc ctttgcacct tgaataacag
    11701 aaatatccat ggatcctttt cccacagtca tttttgcaaa ctcgtctgtg ttaaaatctc
    11761 tattgatcat caataatttc ttgatattca gtttaggttc taaattcaaa attgttccat
    11821 cgtttagttc aatttttgag taatcttcgt tcatttcgct acctccgttt tttttactgt
    11881 gattgagtgg ccgtagttgt cacaactgaa gtttttttaa tcacatcagc agatagattc
    11941 gtcatccatt gatctgttaa gtcttcttca agttctgcaa caattgcttc atgataaaat
    12001 ttaccaaatt catcttgcat aacttttgtt tctagttcta acgcagctac ttcatccgca
    12061 ccattttcaa tagagaatgt taatcctgta ttcgaagtgc atgctaacat accaactaac
    12121 tttctatttt cttcgaagtc atccacgatc tctgcagcaa gtgagaaatc ttcgcctacg
    12181 gaatcaggac cgtaagagta aatgcctggt ttaatacgtc catcttgttt caacccattg
    12241 aaacgtcgat aaacttccat cggtacatgt gcagtaattg ttaccgtcat attgattggt
    12301 ttagattttg attttacttc tgtcgctcca cattttttaa ccaccgtttg catttctgtt
    12361 tcgccatcta attgtccatt acaattcgtt gcgattgcat ttcctgcgtt cttaaaatta
    12421 aaagcaattc gtttgatact tacgttatcg aacgttgtta ctacagtttt tgttttagcc
    12481 attgttgttc cccctattta tttaatttat cgaattgacg aatcagaagt tctgtaattg
    12541 gatcaagtgc aagacctaat cctcttctca taaattcgtc cggctgattt cttttagaag
    12601 tacctatccc caaatcagga tattttaaat actcaaattt tcttgtaggt ctaatgatga
    12661 aacccaaatt aatgtattga gtcttaagtg gacgactatt ttttgcgtgt tggtgccctc
    12721 ttcttaaatc tgcttcagaa acaggaattt tttctgtaat cctatccact gcaatagccg
    12781 aaccttttga tttcaatgct tcgttaatca gtcgttcgct ctcgcttgaa tagcgttcca
    12841 tccgcacaag aagttcatca tgtccattta tttttagctc ccaactattt ttagccatga
    12901 caatcactct tcaataatcg tctaaacgta aataccaatt gatcgatata gcgatcttgg
    12961 ttctctagtt ttaaatgatt gggatccatt ctctgaaaac gaatcgaacg attttgaatc
    13021 aatgaaataa tatctagtga gtctcctgtt aaatcttctc tattttctga atagaaagtt
    13081 agatatagat tttgacccac gctatatttt ggctcagtga tcatttctat ttctcctgtt
    13141 tcgagaatga agtaattaaa atcatcaggt agctcatcct cgcctacgga gtcttgaaag
    13201 agtttgaggc caaaatgctc ttctaaggaa gttttgatag cagaaatttg cttatttaaa
    13261 cgttcttttt ctttagaatt atcaatcacc atattcaccc acactttcaa gataaaaata
    13321 gatataaaaa ttatcgtaat cggcatagat aacgttgtaa cgcatactat cgattacgat
    13381 aaaatattga tctttattaa atttcttggc gattggatga aatggagtct ttactttctt
    13441 agttaatttc gatcccatcg catccatagc tgttatatca ctatctctca tggaaagatt
    13501 tctaaatttt aaagaagtga tttctgtatc ttctacacca atcttttttc ctagttcatt
    13561 tcttttggta gtttgcgtca aaatctttaa ccaaccatcg ttgaatgttt cttcgagtct
    13621 acgattattc gccattcaca tcacctgcaa tatattcttg tagcgcataa tgttgaatga
    13681 aacctaataa ctcactagcg aaattttgtt caaactcatc taaagcacga ttccagtcgt
    13741 atctacatct ttcgattagc aatccgtatt ctaagctttc aggagaaaaa gaaagtgttg
    13801 tactcacttt actttgaaga taaacagcat ttttagctat catcttttta attgactcat
    13861 cttcttcgtt ccaggtaacg taaatattat ccttcacagc tattagcaat tcttcagtca
    13921 cttgttcagg cgtcatctaa ccaccgcctt aattgcttta acatatgcgt aagagcattt
    13981 tttcttgttt acaaatgata aatcttcatc aaaaggcgta gaagtcacgt atctcccttt
    14041 gaaaaataaa tcttcatcgt ttgttgttac tccagcattg tgtaagattt ttacttcttt
    14101 aactttttct attggatcag tagcaaaaca aaagtctaat tcctcgtgaa ctttaggacc
    14161 aatattgaaa tacatcatgt tccaaagctg tgcccacatc tcggctgtcc agatttgtat
    14221 atttgttttt tgccctctaa ggtagcgata tagccgatta gaatccagat aaaccttttt
    14281 ccaataattc gctttaggac ggttaataac ccactgtgcg cctcctgaat tagtgtttat
    14341 agtttccaaa gattctactg taacatttac aatgtttgcc atatctttta gaatattttc
    14401 tccgttttca cagcttctaa tataatcaag acttagataa ctacagcagt cgctacaata
    14461 ccaaacatca tctttagaag gcaatttgcg caaattaatt cttttattga aaatgacatc
    14521 cgaatcgata tagaaatatc ggtcgtcctc acgcgaatga tcttcttcta aatatttcca
    14581 ccataaatat ggtttaatcg aaggaatata ctctttgtcg tcccgcagat catcgtacac
    14641 atgaacttca acaccatatt ccttctcaaa aaaaatagga atctgatcat cgtgtctgct
    14701 gaaaagcaat acgatatctt tgattcctag tttcttcaga ttagttaaac aaacttcaag
    14761 ctcccattta aaccgattga ttgccggctg acaaagaata tacttcattc tgatcaccta
    14821 cgcttgtgtt gtagttgttg tggttgttgg ttttgtagtt gtagtagtag ttcccaaagc
    14881 gctaatatct aatacaatga aactatcgtt acgtttaggt tgaccgtttg catattgttt
    14941 agctagataa atgcgttcgt cttcaacaaa atggtattca tctgaagctt caatttttag
    15001 tgtagatcct acacccatga agtaatctga ggctacccca ataactgctt ttccttctgg
    15061 cacagccgtt gactgcaaat ctgaaactgg tactggcaat acttgtacgt attctccatt
    15121 agcagttagt acagtcttag ctgggaatac tttagaccag taatcagttg gattcacaat
    15181 taggaccaca tcagaaggat tcacattacg ataaatcgga tcattcacac cttcgatatt
    15241 gaattttgat agtcgcgcca tcaaaccgcc catagttaca gcatctaaag ctgtaatagg
    15301 ttctgctttt ttttcagcat attctccgct agtttgtttg ctcatgtcac gcatcattcc
    15361 gactggcata tctttaccag taccatcaac aattgcttgt tctaatgcaa ttctcaatga
    15421 ttctactaaa acagtacgga cataacgatc taaccatact ggacctaaat caagcattgc
    15481 cttacataca ggaatataac ctgatagctt gaactgcttc atgttaatta catcaaagcc
    15541 attatctaaa acttttttaa cagcttcgca aagtttaccc caccatgctg gattgactcc
    15601 acgtgacaca atccattctg ttacaccagt tgtgttaaca aaagtaattt tttgcaatag
    15661 tggatgagat tgttctaaat cttcaaatac acgttcaaat acagtagctg gcactaattc
    15721 ttcgacccct gcaaaacctt cgtttttcac tacttcgtta tagaattttg tttcttgtgt
    15781 agttaataca cgctgaccac ggttcattaa tactaattga tcttgatttt ttgctgttgc
    15841 ttcttctaaa attttatcct gaatttcctt agataagctt accatagctg cgctaaaaga
    15901 ttcttcgtta ccatctttaa aagctttcat caattggtcg cttgcagctg ttacaccttt
    15961 taaattttta actgtcatta ttttacatct ccttgtccaa atgttttatt taatgctgct
    16021 gtaaatgcag caattttttc tgctcttttt tctttaacgt cattcaaaat ttcttcaacg
    16081 ctttgttctt ttttagcttc agtacctgag ctattttctg catcgataat ttcatcgacc
    16141 aatccataac tcaaagctgt ttctgcatcc ataaacgatt ctttttcaag aagttcttgc
    16201 aatgcttcat ctgtgccatt gaatcgtgtt ttatatgaag cctttaccga tttatcaatt
    16261 gattccagtt ggtcagcaat cgtacggaag tcatcgacat ttccttctcc gtatgtggaa
    16321 gcgcggtgaa tcatcaattg tgcattgttg tagattttta tagtatcgcc agccattgcg
    16381 ataattgaag cagcactagc ggctaagccg ttaatcacaa cgttaacttt tgctttattt
    16441 gacttaagta agttcccaat agcaatccct tgaaatacgt ctccaccgtt tgaattaatt
    16501 actacttcaa tttcttcttg atcacctaga ctatccaaaa tatttttgat tcccttgtca
    16561 gtattccctt caaagaacca actagaacca ataaatccct gaataaaaat ttgcggtact
    16621 gcgccttcat tctttactgc tagaaatgtt ttcattgtcg tcattcgcct cacctccttt
    16681 cgatacttgt tgattgtttt tagttataaa tatttcatct gccatcgcct tatcagagcg
    16741 atcatttcca acgcgctctc ttccttcgtt gattgtaaat actccatttc taatgcctac
    16801 atcaatagcg tcaaccaaat ctttgaagct agtaatcttg atcatagttg tatccacacg
    16861 tacaaaattc cctgacaagt attcttctac ttcatagaga ctagcgttaa acgcatcctg
    16921 aataagttca gcaatcggta tgatttcgaa cattaaaaaa gcgtccactt gatccgataa
    16981 cccactcatg tctcccttta gtaggttttt cggaacgtga aacgctgctg ctgtcatctc
    17041 aaagatgtcg tctattaagt tttttatatc tcttgaattg ctttggaagt ttccgctgaa
    17101 atcttctaat gtgtactcat tttgtaattg aaataccgca cctgcattat cagcttccat
    17161 aaaagcctta aattgtgatg tcatcatttt attgatttga tcttgtgttg tattgtcttg
    17221 cggtcggaat aaattccctt tcagtacgta tctacgagcg ttagagcgct tgtaaacatt
    17281 catggcacta gaaatgagtt tcccatacgc ttgataatac gcatcgacta gttgcctaat
    17341 ttgttgatct gcgtatttta tatagataac atcactttct agaaattctc tatcaaggac
    17401 tatgttgtta atttgcactt gagaaaacac atcatctttc aatgcatatt ctgtgacatc
    17461 ccaactatcc gcaataaata tttcgctaga attattagac ggagaaacga tcaatacttc
    17521 attgtagaat attaatctcc tgatcagttt ttttctaaat tctgttgcat tatttttctt
    17581 attaggagct acattcagcc tatagtaaag atcattcttt ttattttttc catcttcata
    17641 tgacttgaat tccgctttac tcatcgcatt tgcaatcaaa tcaatacaag tttcaatcgc
    17701 aaattttcga tacacaaaat caacttgcaa tttacaaaag tattcttcta aaggaaccgt
    17761 tgcttttttt gtgaagtatc ctaccgcctt ttgaaaaatc cccactttct cacctccttt
    17821 caagttagaa tactagagga gtaaatccag ttcctgtatt ttctactgag ctatttgtga
    17881 ctgttacagg agcagaatca taaatatcat ctaaaaaatt caaaccatga aggaatgaaa
    17941 aaaagccatc cgtttttcta gtttcaggtt ctattttttc atagcgtata tttccattag
    18001 aaatatgctc ttcatataca ttcatgcaat accaacgcat aatcgcatcg tcaccaaaaa
    18061 ataaacgttg attaataaaa aggtcatcaa ccagatcttt taacatacca tgtgtaacag
    18121 atccgcttcg aacaatttcc acagtaaaac ctgcttcttc taaagcgggc ttcaatattt
    18181 ttgcacggta catatccata gcgatttttt taatataata tttattactc atttcaagaa
    18241 accaacctac aatataatca gcttctatat tttttccatg aacgatctgt gattttcctt
    18301 gatctataga aatatctata acctctcgtt tgatgttttg taatcgaagg gctgattcgt
    18361 ggataaaagt atgttgtgta aaataaacat ctttatcgta ttttcctagc aacccaacgc
    18421 tggcaaaatc tcgtctatca gcaaaatcga ctgttcctat cacttcatcc attttttcag
    18481 gaaattcttt ttctttcgta tgcagaacat catcatatga agcaacagca aatcgtgtat
    18541 cttccatagg tctgttcatt cgtttggtca tgaacgtaag tcttaaacca gcattacgtt
    18601 gcatttgaga gtattcttga aacattttcc gttttaaatc tgcattgtaa ttaatagttg
    18661 gacaagcttt ttcccacatg tcgggatcat caacttcatt atcgttatcc aagcgacaaa
    18721 taaatggaaa caaactagaa aattctgctc catccttgtc aattccaagt tctccagaaa
    18781 gaatcatttt tgattcttct ataatgtcat caagcggacc accacgaaca tgaccattag
    18841 ttgtatcata aaattctcta taatctcgaa ttttaccacc accagaagta gccacattta
    18901 tcattgaata atcttcattt tcgtgaattt catcaaagcg gtttgcacct ggtcgcttcc
    18961 catcttttgt tctagcattt gccgtgttat aacgaagttt gctgtttgta gcgatatttt
    19021 gaataacttc cttcgtagct ttaaatactt ttttatctaa atcaggatga tctttaatta
    19081 ctttaaatac atcatcaaaa ctagtctttg cttggctttc attattggca tagatatcaa
    19141 tatcataatt tttaataccg tgtttagcgg ttagtagaaa gaagttgttc caagaagcaa
    19201 aaccagtttt accattacca cgtcccatta atgaaagata tctattgaac actagtgttt
    19261 tatctttttt ccatcgaaca ccataaataa aacattgtag aaatttttcc cacggaatta
    19321 attcgaatgg aaagtattgt gctggtatat tgattgaatc ctctaccatc tgcttatcga
    19381 agtaaatatc ttctctagta aagactcttt cttctagata attttttagc aataattgct
    19441 ctttgcatac cttgatagtg ccttcttcta tagctttgaa ccaattttca atatgcttat
    19501 aactcaggaa ttgattcatt tgcttcacct accaattcag gagtaatggc aagtttatcc
    19561 aacatcaatc ccatttgttt gttgacagaa acaagcaacg ctactgattc attcttttta
    19621 ccattctcca gtctaatgcc gttctcggat atatcttctt ccagtgatat cgccgtttcc
    19681 cataaactga tataacgatc aacattatct aagaatggct caatatttgt tttctgactt
    19741 tccaattggc ttattaaaga gcggcgtaat ttttctctgt agcgattttg agacaattcg
    19801 tttttaaaca ttttagccct cctttcatga taaagttcga aaaaatctct tttcctgaca
    19861 gccccctccg tttcatcacc cccaaaaaat ttgcgattta ttttaagggg gggttatctc
    19921 accatcggaa tgaaagcttc agcgaagtca atgtaataat taatctcttc aatactatat
    19981 ccaaaaacat tttttattct ttcgacgtta ttatctttat tcaacgcttc tcttacttga
    20041 ttcactttgt atttactaca acagttatct gataacagat ccctaatacc tacatagcga
    20101 acgtatatca aacgtttaat taatccctga gtataagatg aatactcttc aatcttttct
    20161 gtatcatact ctctgccatt atcattgatg atcatgcact taccaccttt cacttgcatc
    20221 gaagttagca aagctttcta tcttcttctc ttgtttatct aatgctgtaa gatatctgcc
    20281 atgaacttca ttatgatgtt caacacataa acaaataaga ttatctaaat ctaaagctaa
    20341 gtcaggtcta tccttgactt cctttatatg atgaacgttc tctactctat gatacttacc
    20401 tagtcttcta cactcttggc attcatagtg atctcgtttc atcgctttct ctctaagcct
    20461 gcgccattta ggagactgat agaacttaac caaacgatct tctcttgtca actgtaatag
    20521 ccatctatag aattcctcgg tcatgttccg tctcctttcg caatcttatt taatgcttag
    20581 ctattctttt gccatacaat ggaataactt cattgttttc ctttcgttta tatgtatcgc
    20641 tctttattgg tcttctatac tcatatactt tttcgccatt acctttatga acagtgatta
    20701 cttcatactt ctgttctaag tattgtggtc tatacattgt tgtttcctcc tttgtgcaaa
    20761 aataaaaaga ccactcaaag agtgatctaa tatgtaatag taacttgcac gatgcacaaa
    20821 acgcgtacga aattgcgcac ccctatattt ttaaaccgcc gatgcctcgg ttgcttaaag
    20881 tcgctggagt gggattgcac cactcacgag aacttaccag gctctcacga ggctactcgc
    20941 catttaccgc tgcgtcttct acttccgcca cagtgaccga agcttggtgg tgtacagata
    21001 gcacacttac tacattgccg tacgtagcac cgtatagctt cttaacgatc ttttttcggt
    21061 agtcgtaacc gtcataggca tttaatgtcg ctggcaagga atcgaacctt gcatggttgc
    21121 cgaagcattg acctagcaca catgcttagc gtctaccctt tccgccacag tgacactata
    21181 aaattattct tggctgctac tattttttat tttgcccatt tttaaatcca atcatataga
    21241 cattaagaca gagcgcaaaa attgaaatta ttaatggaat catttctctt cacccacctt
    21301 tagttatcgt gtgaataatt aaaaaacaat agacagcaac aaaataacat tgctttgata
    21361 atttggtata aaccactata aatttctttt cttgcaatta tttttaatat atgctagatt
    21421 atcaaccgat atagtcactg cctgtactag cggaaactag tgcaggtttt ttgttctatt
    21481 tactcaaaag ttattacgat aaattaatat tgtgaaaata aatactaagc gtataatttt
    21541 atttatcagc gagtggtccg ctgaaatata aaacaaggat gtgcaagaca acatgtataa
    21601 accctacatg attagttatg atcttaataa cccagggcaa aagtatgata aagtatttga
    21661 aataataaaa gaatttgggg catatataaa gctacaaaaa tctttttggt tagttaaaac
    21721 taatttgaat ccaaatcaaa tgtgtgaaaa attaaataca gtacttgata ataacgattc
    21781 cttattcatc tgtgaactgc aaaaaaatta tcaaggtaga gctacagagg aaaattggaa
    21841 attcattaac gaacatattt tctcttagta aggattagat attttttctc tgtttaaaca
    21901 attgcaaata ccgtcaatat ctgaattaga aacacttacc ttttgctcct tgctactacc
    21961 aatagcttgg agcaattctt gtatttcttc tggagtgcct tctactgata atttcatttt
    22021 tcttccctcc aatacataaa ttaatagaca gcagcagttg aaagacgaca agaacatgtt
    22081 tagttttgtg agtgctgctg tctaatcaaa ataaacagca accgatgaag aatttaggag
    22141 gagttgaatt cacatccttt tcttcataat tagttgctgt ctatctaaga agaagtttaa
    22201 aacgatgagg gagattgcct cccttcgttt attttgtcga tcctgtttcc taatctttcg
    22261 acactaccat aataacactg gtaaatgtct aaaaaccgcc atcattccgc caaaaaaccg
    22321 ccaaattatt tataagcaat tatttttccg tgtttatatg cttctgcaaa ctctattaga
    22381 gcttccgact tcatccgttg tatacttctt tctgaataac ccacttcacg gctaattctg
    22441 tagtttgaga agctatctgg cacacagaag ctgtagtaga gtatctgacg actaatcaga
    22501 ctaagagcca tcaaagccgc tagaatcgca tctctctccg tttctatatc catcatctga
    22561 ataatcgcgt cttctgcctt attgccatgc ttcggtgcct tcggcatatc cgttataatc
    22621 ggcgacttaa tatctatcaa agagcgacct gccatccgct ccaaacgccg aaagttcttc
    22681 agcacatctc tcgcattaca tcttgtctgt ttgaaatcta cctctcgtaa caattgcatc
    22741 aagtcaaacc gctcctttat gtgatataat aaatgtgttg gatttattga atcagtcgga
    22801 gcgatccggc tttttttatt tgctcttgat tacttccatg tcaactaatc tcgctacagc
    22861 taaataatgc ttgtgttttg cagtcgaacg atcacacttc attgtatttt caatacgaat
    22921 gattgctgag tttgacagta tctttttaac gtatcctctg aacggataaa cgaactcttc
    22981 tgcttcacag cggaccatgt caccgacttt gaattttgat ttcttgcgtg ttttagggtt
    23041 ctttgtcggc atatctagca ttaaaccgcc gataccgtga ctgctagcgt aaaatccgtc
    23101 ttttagtttc atctttctac cacctcttcc actgttccat tcaccaacaa tgctgcagca
    23161 tccgctgttg ctttatcagt gaaaagcaat cgatgagatg gattgcattc aaaactataa
    23221 tttactccat cttttcccca taaagcacat ttagtaagat agtttccatc ggcattcttt
    23281 attgcccata acggctcttt ctcaacctcg tagccgttgt ataggctcaa tagtgtttca
    23341 tatgattgtc ttgttgccca agcgagtaat tcttctcctt gctcttgcgt cactatgcca
    23401 tctcgtacaa accaatcaca gaaataatat gaatcaccat ctttagattt gattaaatac
    23461 gctatttttt cagcttttgt aaaagggtcc gtagatttct caagccaacc ggccacgagt
    23521 tgtggaataa ctggtttctg cggttcgtct atttttttca ttaacgtaat acctgaattt
    23581 atactctcgt tatagcaagc agctattgca tcatttccta tcgctttaat actttctaac
    23641 tttttgattg cttcctgttt attcatcgct gttcctccaa taactcgcta ttctcgtata
    23701 tatttccggt gacttcgtac gtgtattctt caaacagctc tgtgttaaaa atttgatact
    23761 ctaagtcttc atcaacagtg gcacaaacta atccggaata atctattgaa tttttgacaa
    23821 tacagacttt attatctaag taatcgaagc catttcgcac gctgactaat actatatccc
    23881 cttcaaatat atccacacca ttcttatctt tcattcctgt ggattgcatg aggtcgatgt
    23941 ttcgcacgtg atatgatttc aattcggtag gatttatcca atattcaata aagtttgttt
    24001 tgccgttttt agtaaaatgc aagacggcta catctctcat tacgttcttt cgtttatccc
    24061 acgctctaaa cttcggtatc attcgctgtc ctcctcgtat ttttcaatca attccattac
    24121 ttttttcact atttcaaact caaccgcttt tgattcttcg aaatcatgaa caatttctgg
    24181 aaacagtaca tcatcaacta cccacaaaat aatctggcgc tttccaccaa aatttatgat
    24241 tagatggtcc gattccacag atagtgttgc tcctgattcg atatcatata aatccatgct
    24301 gaattgaacg agttttttta tcatttgctg tcctcctcaa atactcttct aatatttctt
    24361 tatacttctc tacaaatttg aaacgatctt gatgaagttt cttgctccaa tttgtttgcc
    24421 gatccagctc acgcatctga tcgaaccctt tttgaatttc gttgtaataa aattcaatgt
    24481 ttgctgctgc tttccaatgc ctgctacttc gcactcctgc tcctgtttca gccatttcta
    24541 acttaactaa ttcagctcgt tcttttgatt ttttatcttt ctgaatcttc atcatgattt
    24601 tcttgaggat gatgtcactg tattgtgtaa tgagatccat tatctctcct ccacatacct
    24661 aaactgtcgt ccttttgaat caatccataa gctcctagct ctatcccaga taatgttttt
    24721 gcttaatcca gtaatttcag ataactgttc agcagtacct gttactaaaa ttcggtcatc
    24781 atgccagatt gcaatttttc tcggcgttct ccgtttgggc ttttcagtcc acattgattt
    24841 accgagcttt tggacttctg caactatttc tttgtcttct tgccaagatt ctgacttggt
    24901 taattcagca attcttttca ttgctgcttt cttatccacg ctcattcctc caatcgatgg
    24961 atttcccttc ttaaattctc tatgtgcaaa tcgattgcct ttctcgccgt ttcattgacc
    25021 atcactgcct ttgttcgttc cagatcgtca atctcacgct gaaggcttcg aatacgcatt
    25081 tgaatcactt cttctgttgt catgatgatt cctccacgta tctaaacgtt ctcttcttag
    25141 cgtctgtgta gccacacctt gctcttttcc tcacgatttt ctcgtgcaag cctgtgagac
    25201 ttgctaactg ttcagctgtc cctatcacta gaagtttgtc accgtgccaa attgcgattt
    25261 ttcgtggtct tggcttgttg ctcttgtctg cccacatcgc tcttccaagc ctcatcactt
    25321 ctgaagcagc ttctttgtca ttttgccaat cttctgaata agtcaattcg ataattcgct
    25381 gcattgccgc tttcttatcc atcccgacat tctcctttca gtaatttgag tacttgatca
    25441 agtgcgctct cacgtccgcc atggaacgtg ttgagccact tgtcttcgta cgatacactt
    25501 tgtcttaaag cttcttgatg cattagttca atctgtgctg taaatgtctt taggtccatc
    25561 tgattacacc tgctcaagtt cattaagatg tttttgcaag cctttaacac aatcaacaaa
    25621 tagtaatttg gtatatgcta aatttcttaa ttgtgttgta tcgatataaa gtgcgaaata
    25681 gtatctgagc ttactccaac tcgaacgatc attcttaatt ccttcaattc cagcttcttc
    25741 gagttgatca tatacgtctc tcaggacgtc taattcctcg ccctttttat aattcgctat
    25801 ttcattaatt agttctagat aatcgatctt catttttcca cctcttagaa tggtgctttt
    25861 gattgtctat tagctcgttc tagcgctttt ttcttgagat aggcttcttg gtcgattgcc
    25921 cattcaggaa gcttctctcg tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt
    25981 tcactttttt ctttccttgc ccaacttcga atagttgcca aatagttttt ataagtctta
    26041 ccagatgatt cacaatactc tgacagtcgt tcgattcgct cttggtaatc attagggaat
    26101 tctgttttga gtttctccat ctgctcatct gacaaaagaa catttttata ctctccgtat
    26161 ttatgacgga cgggcttagc cttcgatttt ttcgaaggcg gtaactctct tatatattct
    26221 tttgtattat taaatgtatt attaatagat gtattattat ctttgacttt ttcgtcaata
    26281 ggggtattgc gtttttcgtc aatagggtat tgattaattc gtaggtacct attgattatt
    26341 tgattggtac cctctttgta aatgatttcc cgattcaagt atccaaactt aatcaaatca
    26401 cttacccatc gcgatatggt ctctttattc acaccatata aatctgcaaa gtactcattg
    26461 cctgcccaac aaaagcctct ttcattacac aaggccgtta tctctccgta taacaactta
    26521 gtatttggtt taagtctttt gtcgtacctt acgttggctg gtataatcgc ataataactt
    26581 cgatgttctg tcatttttac cctccaatat ttaacttttt gattgttttc tggtttaatt
    26641 tgatcccttt gatttgatat ttatttttga aattaatcac acctattttg tgcttctctg
    26701 tgtgatggat tctgcagagt gctgcaaatg tgtactctgc atgatcaact tctttgcgct
    26761 ttcgtcttcc tagcgctttg tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc
    26821 agcagactct ttttgtgatg catttgtaga agtaatattc ttgattcgca ggtaaaatct
    26881 catagccttc tttgaaagga atatgatgtt caaagatgaa atctaagatg atatttgcta
    26941 agacattggc atcactcaca gttgtattcg attcgtcttt caggcttatt ttgcgccctg
    27001 tgacaccttc aaaacgaaag tagaagaatt ccttccagaa gtccgttggc atgcctgtat
    27061 cgataaaaat atcgcctatc agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc
    27121 taggatcaat aaaacgaatt tcaatgactc gatcaccatc gtagccgtca tacatcgtct
    27181 ttagtcgatc aatgttcact tcctcattga tggttgcgct tatgtctttt cctttgaact
    27241 ttttcagaac cgctgaatat gaatcgatta atggtttaaa cactcatatc acttcttatc
    27301 taattctttt ctcttagctg ctattgctcg ctccatcaag gcacattgct catagcttaa
    27361 ctgttcaata gtttcaacgt tatcagctaa gagccctaat ttatctgtct gctcattaac
    27421 atattcgatt aaggttttgg tcatatcttt acccatctgc tcattgaaag cttctagaat
    27481 cgtctctagc atgtttaatt tctttgtatc gattctaggt ggtgttggaa tatcttcccc
    27541 ttgaaataca tataatccca gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa
    27601 tgagttattg atttgcattg catttggttt aacaactggt tggtttcgat aatctaaaac
    27661 aggaaataat tcggtttccg tgtgtccttt aaccgttact gagacagata cataagtccc
    27721 agtttcatcc ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga
    27781 aacaaccctg tagtgtttgt actcattaat agttaccgtt gcctgtggat cattcttttt
    27841 cataatctcc cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat
    27901 tttatttaac ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat
    27961 caaattctgc ctccatttca gcaatgtatt tcttacctgg tccgtaataa gagatatcaa
    28021 tcaagttatc tctgtcgtac tcttctagcg catcaatcaa gccatcttcg atgacgtaaa
    28081 tatattcagg tttattcgaa tgcttcgata gatggataag ataaacatga tcccaaatag
    28141 ttacatggtt gcctaaatca tcttgatccc aagctagttc ttcattcgtc aaaagatttc
    28201 gtctgatttt tcgaccactt gtttcctcaa ttttcggctt gccccaatca ggatcaatca
    28261 aatattgatc tagagtggaa agttcttttt ccatatgcta aaatctcctt atgatgtgtt
    28321 ttctttgtga ctctttgctt gccggcggag tcactttttt atttgttgcc atgctttttg
    28381 cttgtcaata tgttgttggc ttaggatgat tggtttatag tatttccacc agcaattagc
    28441 aattgccgtc cctattctta gcgcttcagc tctattcatt gtcatcacca aaaagtctct
    28501 gttgtctgtt cagttgatca atttccatac ggatcgcagt ttctggcaac cacatttcaa
    28561 taaatgaaac agcatcatcg aatctcttac gaggtaactc gccatatctt gggattgaaa
    28621 aggtacgttt aaattcagac caaaattttg agaatacttt tttgctgatt tcttcataag
    28681 ctcggctttc ttttccccct agaactccca taactttcat atttcctttt tgcttaattt
    28741 caaactcttg ttgtccgcta attcgcatag tatctttaag catggaaaca tcttttttaa
    28801 catctttcat ttcttctaat tgatagatca tcatatcttc aattgtttga ggaacagtat
    28861 tcttgcgaat aacatcttcc atttcgttga atgcttcaat gtatttttgt ttgaagtaga
    28921 tagctttctt tcctgtaaac cccatagcca gcaagaaaaa tccatctcta ctaatgaaga
    28981 aaactcgtcg atttctgcca tatgaatctg gttcattacc ttctacaaac atctgtccaa
    29041 aattggacac atcttttttt agtgcatcaa tatctcttaa aacatgttga tgttttttct
    29101 cgaagctttc tgccacttgt aagctcgtag tcacagcttc tttatttttc aaaattacta
    29161 attcttgcat tatttcttct ctcctttttg gtataattta gttaaaaact ggtggtgttt
    29221 attttgaatt tttttcactt aacaattttt caatggatgt caataataag ttttatattg
    29281 agtacgtttt taacagggat aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt
    29341 aatgcttctt acttcgctgc agatatgatt tatacaaaag taatcatttc taactattca
    29401 acagaaccag ctatgttagt taatttagaa ctttcatccg ctaacttaaa ccgcagatgg
    29461 tcagctactc catttaagaa attaattgct aaaggaggat cagtcgatga taatagaata
    29521 tactctgaag ctgtaccgct aaatattcct cctaaaagtg ctatatcttg ttaccttgca
    29581 tttgaagtag ggaaaattaa ttttaataaa ctccttaatc accaaacaaa aatgatattt
    29641 actctaaata gaacccaaat ccacaaaatt gttgatatca aaaatacaaa cttcccaata
    29701 gagaaattag tgaaagaatt aaactgagta atagtaatat acattctagt ttctcttttc
    29761 tattcatttt tagtcagccc cctcggttgg ctttttcgct ctgtactcag cttcagcttc
    29821 atcaagcccc ataaaaatcc aaaccatgta aacaatcgtt cctatcaacg cttgcctgct
    29881 tccccaaagt cctaaagcgt agatgattag cggtgcgctg aacactagcg ctctattaaa
    29941 ttttcccatc cgcttgcctc cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg
    30001 ttttacttct taaaaataga tatgtgtctc ttccttcaac tggataatag acaaatccat
    30061 ttttgttttt ctcgatatcg ataatatttc tatatcttgg gttctttaaa actctagaag
    30121 taaaccaatc atattttctg ttaatccgtt ctagcacttc tggcaacgtc atccatctac
    30181 cagtatcatc agcttttttt aactcctcat aatccacttg ggagataatt acatagcctt
    30241 ctggaattgg gatttttgct tctagatatt gcattagctg ttcctccctt tacaattcgt
    30301 acatagtgat aatcgaatct ataattctgt ttgcttctgc agaagtcttt ttaccgttta
    30361 aaattaaaga taagtagctt ttgctaattc caaatctttc agcaagcatg gtgtaagtta
    30421 agaactttga actttcgaca tattctttga ttttttctct atctcgttga gtgatttctg
    30481 caatgtcagt catactaaaa ctcctttcta accaatttcc tctaaatcca tttgagggta
    30541 atatccttct ttttttagta attcgtagat aaatagacgc cctttctgtg tccatttggt
    30601 attcattaca attttagtgc caccatcggc tttcgggatc tcagttgtat gagattttgt
    30661 gtatccttgg ttcatgtgtt ttttgcacaa taaccattgg ttaccgactt ttttctgaat
    30721 acctagttta tgaagtaatt tattcatctg ttgtggagac atcccataat ctgctgcaat
    30781 ctgactaatt gttactgaat ctgtagaaga taatatgcta tctaaatagg agattttagg
    30841 ttcgtactct gcaatctttt gttctgcgat taatcttcca gttctttctt cttttagttg
    30901 agttgctaat tgaatgattg tgtctggatt aagcaaagct tcttctactt tttctggagt
    30961 tagataacct ccatgttttc taattgctgg caacacttca cttgttaccc aacgtttaaa
    31021 ttttttggca gagggaagtt ttgattttaa gattaaactg tacaaaccag attcgttgat
    31081 gattgtcatc tctcttgatt gacctgaggt cgtgattcgc gaccccatct tatcttccaa
    31141 atctacatgc cgcgacaaag catctttagt gtttgaataa cccaaaacac tcgcaacgtc
    31201 tttgcctaca aaatatggtt catcatttac taaaattgtc cgaacttcgt tttgttcgaa
    31261 attaaaaatt tgcggtgtgt tcatatttct cattcctttc ttttatattc gtaaacaaat
    31321 ttaacaactt ttttctaaat tcaattgaca gtatctgagt aatactctat actaagagca
    31381 taattaaata agacataaaa acattgattt taaagctttc ttggcggtct gcatttacat
    31441 atcaatagtg ttttttgttg tctttttagt tgttaaatct gtttacaaga tatagtatag
    31501 ctcattactc agaattagtc aaattatttc tgcgtatttt tctaaacttt ttttgtaaac
    31561 atttagaaag gttgttttat cgatgaatac ctatgaaata ataaaagact tggcgaagag
    31621 gaaaaagata tctatacggc agttagaaat taattttgga tattctaacg gttatctagc
    31681 aaagtggaaa aataatactc ctaattcaga tgaattaagt cggattgctg actatttcga
    31741 tgtctctgta gactaccttc taggtagaga agaaagagaa acccctaaac atgtggattt
    31801 atcagaagac gatactgttt tttcttttga tggaaaagaa atatctaagg agacaatgcg
    31861 taaagcgatt gcaattgcta aagctttaga ggaaaatgaa tagttggagt gatggtttgt
    31921 atgtatttga aattgaaaga aatgttgagc gaatacaatt tgaaagtagt ttatatggag
    31981 atgaaagagc ctggtttcta ctatccaaaa cccagaatca tttttttgaa tgaaaatcta
    32041 tacggtgaaa ctgcagaagc ctttcattta tctcatgaac ttgcacattt cagcgcctct
    32101 cattttgaat tttcagtttt gtacgatact tctacaactt ttcactcaaa atttgaaaca
    32161 gaagcagata aaatagctat tctaatttta ttaaatatct atattgagaa cgaactgact
    32221 gataaatctc agtttaatct cgaaaaattt atggagtatt attctattca gaataagctc
    32281 agatacactt gttatgctgt ctgccaatgc tattttaaga aaaaatattc ttacgcaagg
    32341 caatatgtat gaatacatca agaattatta aaaaaaccga tttcagcaaa acattaaaaa
    32401 agcccgtgct gcaacacggg ctctttcctc atatatgagc ttctacaaaa aaaatcatat
    32461 cacagaaatg aggaagaaaa aatggaaaaa gagattttaa taaaatctcc aggaaaaacc
    32521 ttgattaaag taactgatga ttctatttca attattagaa aaggatttat aaatttagtt
    32581 aatcaaggta ttaaaggaga aaaaactatc ccctttaaaa atatatcagc cgtacaatta
    32641 aaaaaaccag gtatgagtaa tggttatata caatttacct tgctaggcgg aaacgaaagt
    32701 cgcggaggta tattagcggc cacgaaagat gaaaacaccg ttatgtttac aaaaaaatat
    32761 tggaatgaaa tggaaaattt aaaaaaatat atagagaagc aacaatctat attagataat
    32821 aattccaaag aaacacaaat ttcaagtgca gatgaaatta aaaagtataa agagttgtta
    32881 gatgaaggga ttattaatca agaagaattc gattttaaga aaaaagaatt gcttggttta
    32941 taatattcaa tatcaaaaga gaaagaggat ctattaatgg acggtatatt tggttttact
    33001 ggtattgttt tatttattat aggtttaatc atgttaattg taagatttat caaaaaaaca
    33061 aataagaaaa cacctatgat tctccttatt atcgggatta tttttactac cgtaggtttc
    33121 tcgctttcct catcaaacga ggcaaatgac aataacaaac aggaaagctc atcatctgat
    33181 actcaaacta ctacatctaa cagcagcaat aaaagcctaa ctcaatttga agaattttta
    33241 gagacaaata atcatgactg gggtgctttc ttagatagtt attattctat tactccagct
    33301 actgagcaaa atacagcttt tactaaatat attagtggta aaacatatac ttttgaagga
    33361 actgttatcg aatcaatgac tactagaata gccattatag cagacaaaga gtacgatgat
    33421 aaaagttgga atgatatttc tgcaacacct aaagtttcat atgtaatttt tgcgaaggac
    33481 ttaaacaatg ctgatacctt tacaaagggc gataaagtaa cttttacagg agaaattagt
    33541 tcaagaggat cgaacataga aaaatcctat gctcaatggg atatgattaa tagtaaagta
    33601 agtaaaaaat aaatgaacca cgccccacta agagttagat tgtgcacaat aataaaacct
    33661 atacaaaaga gtgccatcag gagtcacaat gcttgtaaag gaaatgacaa aataggaggt
    33721 aactatggat aatagcaatg aaaaactaac caaaaaagaa tatcttgaat taatagttga
    33781 aggcggtatt ggagcaattc cttatattgg tggcgttgtg cagtcacttt attttgggtc
    33841 taaaaacgag aagcgtttca aacgcatcga gaaattctac aaaaatttaa acgaatcatt
    33901 agaaaacatt cgcgatcaaa ttccagaaga tgttttcaac ttagagaaca aggaccaact
    33961 cattggaata ttcgaaggga taaatgacga ggtagaaaaa tcaaaggcac aaaataaaat
    34021 agatatgtat aaaaatcttt ataagaattg tttacttcga attaacaacg cttcatggga
    34081 taacgaggag tattatcttc ttgtactgaa tcaattaaca tcgattgaga ttcaattgtt
    34141 agcatgctta atgagtcgtg gaaacgaaaa tttcactgga aatatcagca tgtctggcta
    34201 ttcacaagag ctaatagatg gtagcttaaa tagattatct gattttggtc tgttagaaaa
    34261 aacgattagc tccatagtat taggtgatgt cggcaaacaa aatatgggct acaaaatatc
    34321 tcatttagga attcaattta ttaactcaac tcttacttaa gttttctttc taatatgaca
    34381 tctagaccta tcagcaatgc aacaataaaa aaccagacat accaataatc aacaaataat
    34441 ctaaactctg gaagttcagt tattctttta aaaatcactt taatcataat aaacacctct
    34501 gattggatga tctaatacac atccaattat accattaaaa cgacaaatag cacatccttg
    34561 cgccggcaag caaaaaggat gtgcaaaaaa taataaaacc caaacaatgg gcttctttat
    34621 agttcctatt gtatcagaga aagagagctg attcaattat ggcaagatta gtcaaacgtg
    34681 gaaatagttg gcaatacgaa atttcataca aaaaagatga cggaaaatac acgaagataa
    34741 gaaaatcagg atttaagaca aaaggagaag caaaagatgc cgccaacgaa ttagaatata
    34801 acttgaacaa aggccttaaa ggggatcgca aaaatctatt attatcagat tactttgagg
    34861 attggatgca actttataaa gaaggaacag tatctcctat cacttataga aaatacgaag
    34921 atacgttaat gaacataaag aaatatatgc cagcggtatt gatttctgat ttagatagag
    34981 tgggatatca acgcttttta aataagtatg ctaaagacca tgtaaaatct accgttatta
    35041 agtttaataa ccatattaga gcgtcgttga aagatgccgt agaagaagga ttaattccgt
    35101 ttgatccaac tagaaaagca gtaatcaaag gaaaagattc attgaagcca aaagaagata
    35161 aatatttaga ttatgatcaa tttaaatctt taatgaaact cgtagaagaa aacctttctg
    35221 cgcagtactc ttctcctatg ctcgtgttag ttgctggtgc cactggaatg cgatttgctg
    35281 aacttctagg attaacatgg gaagatatcg atttcgaaga tcaaatcatc actattaata
    35341 aaacatggaa ttataaatta aatgaatggg gaaaaacaaa aaacgaaact tcaaatagga
    35401 aaatttccat tgataaacat acgattgatc tcttaaaaaa gtttaaaatc aatcaaaaag
    35461 aattattcga gaattttgaa atcaaaaacc ctcataattt tgtttttttc aacttaaaaa
    35521 atggattagt ttcatcaaac gccgtcagca aatatttgcg caaaaaatta aaagaattag
    35581 ggattgaaaa gcaatttact ttgcatggac taaggcatac acatgcatct attttacttt
    35641 atcaaggagt aaatatactt agcgtatcga aacgtttagg acatagcagt ttagaaacta
    35701 caatgtctac ttatcttcat attgttcgag agcttgaaga tcaggataaa gaaaaaatca
    35761 atactgtgtt cgatagttta tataaaaatg ataactagtt tggcacattt ttggcacaaa
    35821 tacaaaacaa acccctgtac cgcaaaggat acaggggtta tttatacgtt cctgactgga
    35881 attgaaccag cgacctatcg cttaggaggc gattgctcta tcctactgag ctacaggaac
    35941 aatttcaata aatcaattgt agcttgtttg ttttttactt gcaagcttat ctgctaaaaa
    36001 aatcagctta taagttaggc aaaacgtttc cactagtcct taacagatga taaactagag
    36061 gtagaatgaa ataatgtagg gaggtccttt ccaatgaatg ataagccaaa agaaaaagta
    36121 tacgcagaac gcttagattc atctaaaaaa aatcgggaaa agattacgaa agaagcaatc
    36181 caagaccaca aaaaagaaaa ccctaaaaca aaagaaaaca aaaaattcaa atttacgaaa
    36241 aaagaggatt tactttaaca ttttccttca aatgccaaat gaaaaacaaa agggtggtga
    36301 caaaaatatt gtcatcaccc gttttttatc aaaaatagtc attaagaatt ggctgttgct
    36361 gtggcaagat tgagcttaac gtttcttcat attcttttgt cacctgtatt ctttcttgga
    36421 ataccaactc ttccacttta cgatacccta ataatagctg cgtgatatgt tggatacttc
    36481 ctcgaacgat tgggagtttt gtttgttcta cttttgagag atgatttttt ccattttcga
    36541 ttgttacttc cacgattcct tcattccatt tcgcataatg atcttcttct atttgtaggg
    36601 caaatgcagc ttgtctcttt ttaaatggat aattttccaa aaacaaagaa atatctacga
    36661 tcctcgccat catataaggt gtgatcatta cagaagcaaa tggattttct aatagataat
    36721 tttgatcatt accgccgtag ccgcatgaat attcaaatgt ttcaaaagca ccattgtgag
    36781 aacttacgaa gcgaacaagg ctgcaaaaag catcatgatc caaaaatccc cactccgcaa
    36841 taataaagga agacgttgca agctgataaa cgatatatcc cgttgcttcc tcattatcat
    36901 caaaatgcaa agcaaactgg taattgcctt tttgcttgaa tttatacgta taccaccagt
    36961 cttcgcgctt cagaccagct cgttggtttt ccggcatctg ttcataaatt cttctgattg
    37021 cagcttttgc atcttcaaaa tccatacgct ttatttttcc agattttttt tgataaacgg
    37081 cacatcacga ctgtttagtt gataactgat tttatcaaat gattgttcat agccatagcg
    37141 ccgatagaag ggataggaga atggcgccag ataagataag cttactttac gatcacggca
    37201 atcttcaagg atcttttcca tgatctgatt gatcccgcct tgtccccttg cttctggata
    37261 agaagcaaca tagccaatgc cggccattag gtactcttgt ccgtaaaaat tcactggaaa
    37321 aggggtcgcc atgacttgac ttgttagccg atcctcctca tcaaaaaatc cgtaattcca
    37381 agaattctcc gcaagtgtac gaaaacgttc tcgcctttca ggtgtatctg ccccattaaa
    37441 tgcataagtg gctaaatcaa acatttcctc aatcgaagat acacctagct ctcttacttt
    37501 gtttgtcatt ttgctgcccc ttcctacaaa aaagctagtg aggattcgca ggaaagatct
    37561 atcttcctat gaaaaattcc ttctagctta cttcattttt caataactaa atagtaccat
    37621 gtttcattga tttaatacgt tgcttcgatc atatctagca tttttttgaa acgtctccgt
    37681 ttaaaataca tcaagattgt tcctaaaagc atatcattca acttttgaag catgccataa
    37741 gactccattt tttctgtgta atgaacctca caggtcgaat cgtccaatgg tttgatttga
    37801 taatgaacga cgaattcatt cttcgttgtc gatgtacgaa acttataaga ctggttttca
    37861 atgacttctt cgatttttat gcgagcccgg ttattttgcg agaattgttt gacgtactcc
    37921 aaatttttca gctgttttct agttactgat ttgccggtcg ctttacgtac atcgaaaagg
    37981 acagagtcca taactttatc gtaaaaaaac gatgctggaa tatttaattt tttcgtgatt
    38041 tccattagtt atttttctcc tttgcttctt ctcttttttt ccccttcatg aagaaataga
    38101 ttcctaatcc tgttagaaaa agacctgtga ccatactata caaatcatac ctttgcgaat
    38161 gtggattcaa actatacaac acgatcaaag aaccaatact acataaaaat aaaccgtttt
    38221 ttatcatctc aaccatccta tctttttaaa ccattttcta aacagctgtt gattgaaaat
    38281 aaaggactgt tccccaaggc tgtgctttga ttagttcctt tgctttttta gccgcaactt
    38341 ccggatcttc tgcacttgtt tcaaagaaca gcttgattcc ttgtttttct ttaaatgtaa
    38401 aagtcattcc ttcttcattg tatccttcta acaattcttt gatttcatct tgttgcattg
    38461 cactggcaat tgtaatcatt tcagttcctc ctcgaaaatt cattatgagg aacgaaactt
    38521 aaagcgtccc gctcctcacg ttcatcttat tgttgttcgc ctgcggcagg gtccattttg
    38581 tttgcagaga tgacaaatgg tgcccaaatc aagaaagtca cgaccataca tactaatgtt
    38641 acgatcggtg cccgccaatc cgctcctgtt gctaagaatg acaataagaa cggcggtact
    38701 acccatgtga cttgctgcga taccggagcg actaggccaa gataagtagc tgtccatcca
    38761 attgctgttg ctactactgg agcaatcaag aatgggataa acatgattgc attcaatacg
    38821 attggcagac caaacatcac tggttcgttg atattgaaga taccaggccc aatggataat
    38881 ttagctactg ttagataatc tgcacgttta gagaaaatca agatagctat cagtaagacg
    38941 atcgttccgc ctgatccgcc aaaccatgca aaagcatcga acgatccgcg gacccacata
    39001 taagcttttc catcttcgat tgcatctaac acggctggtg taccagtttt tccgttataa
    39061 cctttttgga aaatatcaat attgattagt tgtgcttgtc cccagattcc ctctagcact
    39121 ggtgccaata catttgatcc atgaatacca aagaaccaga atacttgaac aaatactgtg
    39181 acgatcaata ctgctcccaa cccttgagat aatcctagga atggttctgc aatatatttt
    39241 tgcaccaaat caatgatcag ttgcccatca gctacacgac ctacgatata attgataata
    39301 gcaatgacgt ataaagcgat cgtcgctggt aaaatggctg caaatgcttt agatactgct
    39361 ggtggtactg aatcaggcat tttgatcgta atgttggcaa gcatcaattt acaaaaaata
    39421 atgactgaaa tagctcccat gatcataact gtaaaataag cgtttccatt caaatgatct
    39481 agttttagcc aaccccataa tccaactgac agtgtgccat ctgcaatcgt tgcaccaatc
    39541 tcagaactat tgattgcatc tgcgacattt gaaggaacat tcacatctaa tgctgctgat
    39601 aatgctggac caaaggaaat accttgaatc aaggtagcta aaccaacgat ccccccagct
    39661 agatcattta ctccataagc tcgggcaaga ttataccccc aagaaaaggc aaagatcaat
    39721 ccggcgattg ctagtgtacc gttccagaca aaaccattga ttccgatgat gacattcaat
    39781 acggcaaaga ttcctttgtc tgctgctaaa ctgtcagcat aggtactgtt aaactgttgc
    39841 ggtaaatcac gaataattgc attgatcatt acagcgactg atcccgccat tgttgcgggc
    39901 agtatgccga taaaagcatc acgcaaagca actaaatgtt tttgcgctcc gatttttgcg
    39961 gcaaccggca ggatgtattt ctccatccaa cttgatagtc cgttcatttt ttacttcctc
    40021 ctataagttt tttatataaa gagagttctc cttataacaa aataaaaaat ccgttcaaaa
    40081 atttttcttc ttccgttcaa aaaagaagtt gtatttattc gctgtatacg cgacgataaa
    40141 gttcgatgat ttcttttgct aaatcagtaa acgcaataga tgtcattaag tgatcttgac
    40201 tgtgaacagt caataatgtc acttgcatat gatttccttg tgcttcttgt gtcaacatgt
    40261 ctgtttgtgc atgatgcgct tgtataagtg attcttctgc atcagcgatt ttcctatccg
    40321 ctaactcaaa atcaccgatt ttagcagccg caatcgcttc cattgcatcg ctctttgcat
    40381 taccgccaaa catgattaac cccatgatgg cttctagatt ttgttgatct tccacgttgt
    40441 tataccccca actgcatact tgatttattt cattagattt tctgcttgtg caagaacttt
    40501 ttctccgttc atcatgccat agtcttgcat attgatgaca tctaatggaa tacctttagg
    40561 cgcaagtttt tgttcaaatt gagctttcat gaaacgtact tgaggaccta ataataatac
    40621 atcgacattc ttagattcta aattattatc tgcatctgag gcagagaccg caaaaatatc
    40681 ggcttccatc cctttttctt ctgctgcttt ttgcattttt gtgaccaata aactcgtact
    40741 catccctgct gaacaaacta acatgattgt tttttttgcc atttttaaca ctccttttat
    40801 ttttttacac ttttatataa cgcaagaagc atgccaataa aaaaaagagc tgaaatagcg
    40861 ttttcatgct ttttagctct tttttatagt gcgcacacag atgtttagtg tgtaaacagt
    40921 atgattagtg tgttcatttt tttattttat tttcaagaaa catttgtgta agaaatgcga
    40981 tatcatcttc tggaattttt acctgatatt ttcgttctag tgccatcaag tttgcccgaa
    41041 cgatatccat ttccaaacga taaatttttg agaaaataga cagtttctca aattgccgat
    41101 ggctttcttg tttgatcaat ccgtctaaca tgaaggctaa atggataacg attccagtat
    41161 ctactccagg ttccacgata atcgacaaat cggtctggat ctgctgaact gtcttttgca
    41221 atgaacggat cagttcatca atagaaaaga catcgcttaa tgatccagca agtgaatcga
    41281 caattttagc tgtcgggatc tcatcatcag ctatacgctt taaaatattt agtttttcat
    41341 catcaaaaac gtcataggct gaaaaaaatg ggatattttg ataatcaaca tcaactgttc
    41401 ccgcaattgc tttgatctca tattcttcca ataaggcatc gatgtgtttt ttgaatgatt
    41461 cacgttctaa aaactgcatc tggatcagtt ctaccttatt ttgattaatc actggcataa
    41521 tacgctgata taatttggca gctactcctt caccagtaaa gcatgtcacg attacggctt
    41581 ttttacgctg taaagtttca gacttttccg ctttgaactg atcacgtacg atcgattcaa
    41641 aggaaagctg gatattttga taaatatctt ctaagcttct tccaacactt gccatccgta
    41701 tcgcttctat cacaatcatg gtacttgtca tcgtgatagc tttcgtccga atgccagtct
    41761 cttcataaat caaatcactg aaggtgttta atgatcccat atctgttaac aagagtaggc
    41821 cattcgtcaa atgctcttga tagtttttga catattgtaa cacttcttca tacattgttt
    41881 gaacttccat aaccagtggc atattaaaag ctttccctac ttttgttcct aatagttctt
    41941 gtgccatctc caacatgctt gaagcagtct gtctgccgtg catcaatatg agaacatcta
    42001 ctttgttttc tttaacaggc tcactttctc ctagattgat agaaagaaac atactaataa
    42061 agccgatttc atcaaaagga atctcaactt gttcttcttc ttcaatgatc gttgaaagat
    42121 cgattgctac ctggaattcc ctcttaaaat ttttccgtac gttattcaaa tctgggtgga
    42181 cgatcatatg accttctctc acacgatcca atgtactttg cagatgcaaa gcaaaagcaa
    42241 aacgagcttt ttcattataa atacggtcta accgtttttc agccacatca tacaattgat
    42301 tggttaaccg ccagatttca tctggcaaaa gatctttggg aatcgaactt ttagtcaatt
    42361 ctttgacata tgtctggaaa taagcatcca catcttttga aatgagggtc tctagatcaa
    42421 tgttctccac accggtttcg gaaagagaag tgactttttc ctcaatatcg ttatagacct
    42481 gcatgtttcg ttctggatct tgtgaccaga caacatcagc aaatcctggt tcaaatgtca
    42541 aatactgact tttgctgtcc atgaatcggt cgagacgttc cggtatttcc ttgattttca
    42601 gtaatccttt ttgtacttga agggaaagtt cttcttttcg aatgattagc tttttctcat
    42661 gatgagtacg gtaatgtaaa aaagattttg cacagactag tttcaagtct cgtttcactt
    42721 gaccgatatt tccttccgca tcataaagca tgaaggcaag aatcgcttct ttttcgatat
    42781 cgatacgttg attcaaacga ttcgcttctt gtttgataaa aagagagatg atttcataac
    42841 gctcatccag tgaacgtgaa gcaagattcg gcaaggtgat tgccatagga atccgtcgat
    42901 tgaacgtaga taagaaactt tctgaagatt cagtcgtcgc cccaatgatc tgaacagatg
    42961 cttcatgtac ttgtgcactt tctccaagcg gacggtaaat acctttatca atgaaagtaa
    43021 acagcatttc ctgcccttcc ggcggtaaac ggtggatctc atctaaaaac aatataccgc
    43081 cgtctgcttt ggctattaga ccgggactat cttgagctgc tcctgtgtaa gccccttttt
    43141 tgatcccaaa gatgtgtcca aagagcagtt gcggattttg agcataatcc gcacaattga
    43201 aagaaacaaa tggcgcatct gcagaaagca tctctgaatc gattgcaaaa tgatacatac
    43261 attcagcaaa catcgacttc cctgtaccag tttctccaaa aataatcgta tgcaaccccc
    43321 gcggtggata aagaatcgca gcttttgcct gctgtatgct gacttttaaa gaatcatttt
    43381 ctccaacgag tgtttcaaaa gtcacttgcg tagaagagtc gacatgcact tcatcagatt
    43441 ttgacttttc cgttggcaaa gagtaaacga ctggtcggcc agcggttttt acaatatgat
    43501 gggccttgta tagttcactt aaatatcggc ttgcattgct tcgatcgata gttaagcgct
    43561 cagctacatc acttgctgtc aatccttgtt ggttttctgt caatagctgc aagatctctt
    43621 cttgacgttc tttcacataa cccctcctct ggcaaccaat tttgttcccg ttttcataat
    43681 catagcataa aaaggcgctt atttgcgcct tgctccttta tttctctctt tttttgattt
    43741 atttttaact ttttttctta ttttttcttt gttatattct gggttctgct cctttccaga
    43801 aaaagttttc gcatactttt tatttactgc aggagctgca tcttctttgg ttggctgctc
    43861 agtttgcagc ttacctccgt ataaaaaaat ctcttcaagt gttatatttg atggacgtaa
    43921 aagccgtttt aaatctctta gattattatc ttgtacgagt gtaattacta ttccttcatt
    43981 acccattcga ccagttcttc ccgaacgatg caaataagct tcttcactta acggaacttc
    44041 tgcattcact acataaggaa gacctgtaat atctaaacct ctagccgcca catctgtggt
    44101 caaaagtgct ctgaccattt tctttttgaa ttgttctagc gctgcttttc ggagtagctt
    44161 gttctgatca gaggccagac ttcctaccgg tacacctcta tacagcaatt tttcttccat
    44221 tacgcctaat tcagtcagtt gattaaagaa aatcagacca tgaaaatctt ctatatgcgt
    44281 gagacgtctc aagtagtcat ctttctttcg tatcggtaca cgtaaaaagt aatgtctcag
    44341 tccttttcga gaatcgtcct ctgctgtcac gtcaattgtc tcaaacgttt tcccagtgat
    44401 tttttcaatg ctttcaaggg aacgatctgc tgtcgctgaa aaaaaagcta gctgatactc
    44461 tgttggagct tgatgaagaa tttgatcaat gatttggctg ttcccagcgt caaatagctg
    44521 gtctgcttca tcaaatacca ttgttttcac ttgatgtgct tttatttttt ttgctttcat
    44581 caattccaat attcttcctg gtgtaccaat caagatttct ggtttttcct tcaaaccctc
    44641 aatctgacgt ttgacgttgg ctccgcccac caaagattgg actttcaact ctttgtcttt
    44701 tccccattca cgtgcaacat ctgctacttg aatagctagt tcttgagaag aagcaaaaat
    44761 gaccaacgca cttgcttcac ctggttccac gtttagtagt agaggccaaa gataagccag
    44821 tgttttacct gaacctgtag gagaaaccgc tactatactt tgtttattac ttagtggctg
    44881 aaaaactgct cgttgaataa gagaaggtga gataaaccct ttatcctcat attttttctg
    44941 ccaattttcg ggaaatactt gtacaaattc gttcattaaa aatcttcctc attatctgca
    45001 tcgaatttga tgccgccatt ttttcttaaa tcataaatca cttggttcac atttctagct
    45061 agctcgatcc actcttcata caacaatccc caagctggat ctttcggatg agccatgaca
    45121 ttggcaaaat cctctgcttc atctgccatt ggattttctg cagctgttac cgccaaatca
    45181 atgatttctt tatgcgcgcg atcatgaaat tgtgcttgtt caattgcatt gactccattc
    45241 atcactagcg ttcctgagtc aaaataaatc tctgacggta aataagaatc accgatttta
    45301 cctggctgga tcgatacatc aaaattctca taacgtaaga tcccccatcc aagaccatct
    45361 acaccagtag aaatctttct tgggaaataa tggacttctt ttggcattcc aaaccaagca
    45421 acagctgcat atacaaggta aacacctaaa tccatcaatg caccacctga gaaatgagtc
    45481 gagaagatat tcggttcttc tccatctaat acttggtcat agcgcgaaga atatttcata
    45541 tagctgaaat ttgcaccgat tatctcgtct tttaatggta agtaatcggc aatcttttta
    45601 aaacctgatt catggatatt tcttgctgct tcaaaaaaat acacattgtg ttcatttgcc
    45661 aaatggatga tttcatccat ttcttttggc gtcgaaaaag ctggcttttc aactatcaca
    45721 ttttttcctg ccaaaattgc ttgcttagct tgactaaagt gaagactatt cggagaagca
    45781 atataaatgg tgtccatatg agtaatattg aaaaaagtct tcaaatctgt tgcgaattcg
    45841 atatcccctt cataatgaga accaaattcc tgtgcttttt ccaaatgtct agaatatacc
    45901 gctgtcaatt catatttttt tgtgcttaaa gctgcttgga caaattgatg agtaatccaa
    45961 tttgttccga taatacctaa gttgatcatt attttacctt ctttctattg tataggatct
    46021 tcaaaaggga tatctacatc aaatggaatg gcctgcttga cagttacctc atctgccgat
    46081 acttgacaat tataggcgag gatttgaact ccgctttgct gcgcatgtcg aaccatatca
    46141 gctagtgctg gttgcatttc ttcatggatc gttgccagat gaagatgttc gaactgagca
    46201 ataaacaaga cataagcggc ataaccatct tgtgcggcat acgttaattc tcctatatgt
    46261 ttcaatcctc tgagtgtggg cgcatcagga aaagcaccaa tcgctttatt ttcaagtgtc
    46321 atacctttga cttcaacaaa tgctttttgt ccgaccaccg tctctaaata aatatcaaat
    46381 ttggagtgaa gataagttac ttcacgcttg tataccgtaa atggaccagt taatcctggt
    46441 aaaaggatcg tgccgtctaa aataccatca attgccagcc gattaggcaa ttgactatcg
    46501 atattaaacc agtcaccctt cttttttaca gcaatcagat cataggcggt tttccgttta
    46561 ggtccaggag caaatacaat agccacttca gcgcctggta tcaggacctc ttttcctcta
    46621 cctgtatttt tgacatgcgc aataattatt tctccgtctt ttttcataca atgtgctata
    46681 aaccgattcg ggcggtcaat aaaaaaagct atttccactt gctgatactt caaattatgt
    46741 cacctttctt cctactatgt atcctatcat aatttgtaga aaaacagtaa aaaaaccagt
    46801 cgaaagtctt agagaaattt tagcttttat tcggtacaat gaaataaaga agaaagaata
    46861 tgtctatgcg attattttct cataaaggag tgatttggtg ataaaaaaaa tgatctattt
    46921 tcttggttca ctcattaccg ttctctcaag ttttatttac ttgaaaaaga aaaaacaagc
    46981 ggaaaagtca gaaggactct caaagtatca aggtacatgg ttctttagtg atagccagca
    47041 aaaacaacaa ttacagatta catctgatta cacgtttttg atcaacggtc agtctcttgg
    47101 cacatctgtg gtagaaatag atgaatatcg tttagtcgta cgtgatcagt atggttttta
    47161 tcttatcttt tcttatcccg aacaaactct ttatgacgaa tctgcagaca cttcatataa
    47221 gttgttatct gaaaaataga tccctcccaa tgtatatata tatcctttat aaacgaaaga
    47281 atgagtcttc tctatcatct ttcgtttttt gttgtaaaaa cgtctaaaaa tcctttgttt
    47341 tttatttaga aaaatagtca attaacgttt tttttgttac acttactaat gatggttttt
    47401 taccatgaaa ggatgatcga atatgacacc aaatcgagaa gattatttga aaatcatttt
    47461 agaactaggt ggagatacaa cgaaagtcaa caataaacaa atcgtatcta gtctagatgt
    47521 ctcagctgca tctgtcagtg aaatgatttc taaacttgtg aaagaagagc ttgtagaaca
    47581 ttccccttat caaggtgtac aattgactgc aagcggactg caaaaagcta gcagtctgat
    47641 tcgcaaacat cgcttatggg aagttttttt agttgaacat ttagactata gctggaatga
    47701 agtccatgat gatgctgaag tattggaaca tgtgacttcg gaacatcttg cagatcattt
    47761 agaaaactat ttgaatcatc ctgaacattg tcctcatgga ggatcgattc ctaaaaaaga
    47821 ggaagttgtc catgaagaac gccgacaaac attggagtcc tatcctgtcg gtacaaaagt
    47881 gcgtatcgca cgagtgcttg atgaaaaaga attgttggat tacttagtca ctattgacct
    47941 gaatatcaac gaagaatatg agatcgttga catcgcagct tatgaaggac cgatcactat
    48001 ccaaaatgat agaaagaaaa tccccgtcag ctttaaggcg gccagcacga tttttgtcga
    48061 taaaatataa aggagtgtat ccacatgtct aatgaaaaaa atgtattgtt tacgatattc
    48121 ggcggtacag gtgacctagc ccagcgtaaa ttgtacccgt ctttgttccg cctttacaga
    48181 aaaggaaatt taggcgagca ttttgctgta atcggaaccg ctcgccgtcc ctggagcgat
    48241 gagcactacc gtgaagttgt caaagaaaca atccgtgcat tgaatccttc agatgaagaa
    48301 gcaactacct ttgcaagcca tttttactat caatcccatg atgtaaatga ttcaacgcat
    48361 tatcagacat taaaagaatt gtctgaccga ttagatggac actatcaatt aaacggtaac
    48421 cgtgtctatt atttggctat ggcacctcaa ttttttggaa cgatcgtttc tcatttgaaa
    48481 tcacaaaata ttatgacaaa aaatggcttc aatcgtttga tcatagaaaa accgttcggt
    48541 tctgattacc agtctgcttt tgaattgaac cagcaaatca gagaagtatt cccagaacaa
    48601 gatattttca ggattgatca ttatttagga aaagaaatga tccaaaacat ctcagctatc
    48661 cgttttgcca ataatatttt tgaggctcaa tggaacaatc gctatatcga caatatccaa
    48721 attacttttg gcgaaaacct cggtgtagaa gatcgcgggg gctactacga tcatagtggc
    48781 gcacttaaag acatggtgca aaatcatatt ttacaggttg tagcattact tgcaatggaa
    48841 ccgccagtag cattttccga aaaagaaatc cgtacagaaa agatcaaagc attgaaagct
    48901 atccgcattt ataacgaaga agaagtgtta gaaaacttcg ttcgcgggca atatgctcaa
    48961 ggtgaattgg atggtgtaca attcaagggc tatcgcgaag aagacaaagt ggatgctcaa
    49021 tcagagactg aaacattcgt cgcaggcaaa ttcactatcg ataacttccg ttggtcaggt
    49081 gttcctttct atgtacgtac aggcaaacgt ttgactgaaa aagggactcg gatcaacatc
    49141 gtattcaaac aagttcctgt taatgtattc aaaacatctg tcgatgaacc atgtgacgat
    49201 acgacacttc cacctaatgt tctaacgatc tatattcagc caactgaagg attttcttta
    49261 tccttgaatg ggaaagaagt agggcaagga tttgaaacgg aaccaatcaa attggaattt
    49321 agaaatagtg cagaaatggt tgaaaatagc ccagaagctt acgaaaaatt actattagat
    49381 gcgttgaatg gtgatggaac gaacttctct cattgggaag aagtcgctca atcttggcat
    49441 atcgtggatg tcattcgaaa agcttgggac caaacacaac cggattttcc caactacaaa
    49501 gctggatcaa tgggacctaa agcagctttc gaactacttg aaaaagacgg attcgaatgg
    49561 atctggcaac cagataactg gtatcgtgaa cgtggaaaat taaacgattg ataacaaaag
    49621 ggggtgtgac aaagaacatg tcacaccccc ttttcccttt tacttttctt cgaatccttc
    49681 agctacagct tcaggataga agtcttcaac gatttttgca caacgaccgc atagatgagg
    49741 aagtttttca tctactccta catctttacg aacttggcga cagcgatcgc aagtttcccc
    49801 gtctgctttt tccactgtaa tagcaacatc atcaaatacc atagcgttat ctggcacttg
    49861 ttcatctgct gccataactt caaagtagtc tggtgaaacg atcaataatt gtgcaatatc
    49921 tgcatccaaa gcttttaaca tttcacgaac ttgttgtttt ggataaacag ttagttttgc
    49981 ttccaaagat ttaccaatca atttgctgtt tcttgcttct tccaatgctt ttaagacatt
    50041 atcacggaag tccatgaaag cagtccaggt atccatcaat tcttcttggt ttgcaaactc
    50101 ttcataacca ggaaactcgc ttaactgaac gtactcctct tcttctttca ggaatgacca
    50161 gatttcttct gctgtatgcg gaatgatagg agtcaataat tttgtcaaag ctacagctgt
    50221 ttgatagaaa acagtttgca tgcaacgacg ttgataatca tcttctgctt caatataaac
    50281 tacatcttta gcaaaatcca ggtagaatga cgatagatca accgtcaaga agttcataac
    50341 tgtacggtaa atatgcatga agttatattt gtcgtaccct tcttctcgga tttctttgat
    50401 tacttggttc aatcgaacgg tcatatattt gtctacagaa cgtaaatcat tgtacgaaac
    50461 agtatctttt ttaggatcga aatcttctgt attcgctaat aagaaacgca tcgtatttct
    50521 gattttacga tatacttctg ccacttgact taagatatcc atagaaacac gaacatctgc
    50581 ttcataatct acactagata cccataagcg aaggatatct gcaccaaatt gtttgatcac
    50641 tttttctggt gcaatgatgt ttcctaaaga tttactcatc ttacggcctt caccatctaa
    50701 agcaaatcct tgagaaataa ctgatttgta tggtgctaca ccattgatag ccacactagt
    50761 cgtgatactt gagttgaacc agccacgata ttggtctgat ccttctaaat acatatctgc
    50821 tgggaatgat aattctggtc gctgacgtaa aacagcttca tgagaagaac cagaatcaaa
    50881 ccagacatcc atgatatctt tttcttttgt aaattcgcca ttaggagaac ctgggtgggt
    50941 aaatccttca ggaagaagtt ctttcgcttc tttttcaaac cagataattg atccatgttc
    51001 agcaaataat tttgccacat gttcgattgt ttctggtgtg atgattgctt ctccattttc
    51061 agcatagaaa atcggaagtg gtactcccca tgcacgctga cgggaaatta cccaatcgcc
    51121 gcggtcacgg atcatattat acaaacgtgt tttaccccat gggataaccc actctacttt
    51181 ttctacttca tccaaaatat tctgacggaa tttatcgata gatgcaaacc attgtggagt
    51241 tgcccgatag atcacaggct ttttcgtccg ccaatcgtga ggataactat gagtaaagaa
    51301 atctagcttc agcaaagctc ctttttcttt taatagatca gtgatcatag gatttgcttt
    51361 gtcataaaag ataccttcaa atccaggtgc ttcttctgta aacaatcctt ttgagtctac
    51421 aggtgacact acagatagtt tatatttgct accagcgata tagtcatctt ccccatgtcc
    51481 tggagcagta tggactaatc cagtaccagc gtctaaagta acatgatcgc ctaaaatcac
    51541 aagtgattca cgttcataga atggatgact tgcagtcata tattccattt cttgtccagc
    51601 aaattctttt agaactgtta cattttccca accgatttct gattggactg tttctagcaa
    51661 atctttcgct acgacaaact tacggccatc ggcttccacc aatacatacg taaattttgg
    51721 atttacagca ataccttggt ttgctggcaa tgtccatggt gttgtcgtcc aaatgacaaa
    51781 agctgtatct tcatcaagaa tatctttgcc gtctttgaca ttgaaagcta caaaaataga
    51841 tggagatttt acatctttat attcaatttc agcttccgct aaagaagact cacttgacgg
    51901 agaccagtaa attggtttca atcctttgta aatatagcct ttttcagcca ttttaccaaa
    51961 tacgcgtact tgtgcagctt cataagaagg atctaatgta acataaggat gttcccaatc
    52021 tcctgcaact cctaggcgtt tgaaatctgc tcgttgctta tctacttggg aaagtgcata
    52081 ttcacgacat ttttccaagt attctgccat ggacatttct ttacgtttca cgcctttatt
    52141 agccaatact tgttcaatcg gcaatccatg tgtatcccat cctggaacat atggtgcacg
    52201 atagccagac attgattttg aacgaatgat aatgtcttta ctgattttat tcaatgaatg
    52261 tcctaaatgg atattaccat tggcatatgg aggtccatca tgaagaacaa agctaggttt
    52321 tccttcgttc aactcttgtc ttttttggta gattcgattt tcttcccatt ctttttgcca
    52381 ctcgccttct cggtttggca aattaccacg catagggaat tttgtttttc ctaattgcaa
    52441 ggtttctttc attttcattc aaccatacac tcctttattt gttttccgga cacaaaaaag
    52501 catgttcagc cgcaagggac gaacatgccg tggtaccacc caaatttagt tgcatctatc
    52561 tagtgcaaac tctctgtgac caataacgag gtcagccgct gcttcttact ctaatttcgg
    52621 aaaagcaggt ctcaagtaga ggatctttca ccatgcagtg tcttaccgaa cttccactct
    52681 catcggctcg ctttaagaat aacaaagtgc ttttctgttc tactcgtttt cattttcacc
    52741 aggcacgatc gagatatcaa tagcctgtgt atcaaatgcc gtcataggtg cttctatgac
    52801 tcctgtgtct gagtttacat cagtatcatt ttcattgtca agctgttttg ctaaaacttc
    52861 tttgatcact tcatggctgt cagtcacgta gctggagaat ggttgtaaaa tttcatccca
    52921 ttccggactt ttcacttgtt ctaattgaga ctcaagcatc aagctgatac gttgatggaa
    52981 tactcgtgtt tttttcttaa gatcttcagt ttctgtcgca agctgtcttg ctttttctgt
    53041 tgcatctgtc aagatttttc ttgctttttc ttctgcatca gttgttaatt gatgtgcacg
    53101 tttttcagcg ttcgcaacta actcatctgc tttgttttgt gcagaagtaa cgatcacttc
    53161 ggattccttg cttgcacttg tttttacttt atcagccgta tcttgagcta cgatgattga
    53221 ttggttcaaa gcatctttta attcattgaa gtactcaagt ttttcttcag aatgtttgac
    53281 tgctttttct aattctcgat ttctttgagt caattcctca tagtcacgaa caactaaatc
    53341 taagaaatca tctacttcgt cttgattata tccccgcatt tttgtcggga atgtcttatt
    53401 ttggatatct aatggagtta aagccatgag taaaccacct ttttatttta tttacgtaat
    53461 acaccgaaca gtaccctgta cttttctttt ttcgtcttcc cctcaagttc ttggatctgg
    53521 actctgccat aacctctgac tgaaataata tctaatagtt caagtacaaa atcaggtctt
    53581 gtattttctg tccagttcac ttttacttta ccagcttcga tcagctgctt ggaccgctgt
    53641 ctggaaatat tataaatctc agagatcaca ctgtctagtc gcatggaact aactgttcct
    53701 ctttcttgtg tccagctatc tttcggcata atcatttctg tgtatttgat tggttccaag
    53761 cgtacagcaa cctttccaat ctttgttact tgtgcagcaa caaaatgatc cacctcattt
    53821 gcaataaata cttgccagcg atcaccgtcc gagatgatat cgccaaagta agagcgtttg
    53881 atccccgttc ccactaaagt tccaaggatt ttcccatgac ttaggctggc aaatttgcta
    53941 ggataatgga tttcatacaa tgctgtttca aaatcttcct ctacaggttc gtaataatcg
    54001 gggaaaatca aacatctttt tctttctgcc tgttcatatc cgccataaaa catgaaacgc
    54061 aaatcactgc tttgtcgaat cagtgtttct aaaatatagg attgtcttgg gtcaagaaat
    54121 tcggtcagat agggtgcata ttgtccttga acctgttcaa tccaatcgcc taccgaatcg
    54181 ataaatgtac gttcatctgc ccgaaagtgc tggtatacat ttgcatccaa aattttcccc
    54241 tcctatctat tctgagaata aagattttgt tgcttcaatc aataaactaa atggacagtt
    54301 atccatataa cgatctgtgc taaaccttgt acggctaagt tcaagatcag gattgcccct
    54361 aaaattgtga aatccaaggg tccaatcctc aaaggcagac gatcgaacaa actgatatac
    54421 ggttcgcaga tcttagctaa taatcgtcct agccctgatt gatacccgcc tggaaaccaa
    54481 gagagcaatg catataccat caagataatg ctatagaaat aggctgcccg ttgaagtaaa
    54541 tgaagtaata gtgcaatagt ataaatcgta gttcctcctc ttacagatca aacaaattcg
    54601 catctgcaaa agattgagcg gttgaactgt caatctccat cccagtcggt gtacaaagaa
    54661 aaatctcatc tccaacacgt ttgatatctc catctagtgc ataaactgtt cctgttagaa
    54721 aatcaacgat tcttctcgct tgtttttctt ctactaaatg aaaattcact aaggttgctt
    54781 ctccagcaat gattcgttta gcaatcgcca tagcttcaga ataagctctt ggttccatta
    54841 cagtgatttt tccagcaaga tttgctttag gttgtgtaga ggttcttgaa gctgttcttt
    54901 ctttcgctac agtttttgta ggtgattgat gtagttcaat tacttttttc ttttcagccg
    54961 gctgaatact ttcatgcgtt acaggacgtg aagtcgccgt tttttgtttt tcatgagtat
    55021 tcgcagcaga tacagaatga gttgattgat tctgtgttgt atttttttta gttggctgta
    55081 cagccttttt tgttgtttca ggataatctt ggtattcgta ctcttcctca tcagctaatc
    55141 cgaaaaaatt agcaattgct cctctgttta aaagcggcat ttttgttcct cctttattcg
    55201 tctttgaata gcgctgttcc tacacggata aatgttgcac cttcttcaat cgcaatttga
    55261 aagtcttgac tcatccccat acttaattca ctacatggtg catgggcata accttttgct
    55321 tgaacaagat cacgcaattc tttcagcgta ccaaacactt tatggatttg ctcatcagaa
    55381 gcttctttag gcgccatagt catcaaccca acaaccttga tttttgtgca ggcagctaaa
    55441 gattccacaa acggtaaaag ctcatctggt gaaattccat gttttgattc ctcaccagaa
    55501 acatttactt caataaaaca ggataattct ttctctaacc gcttctggat ttcttctgct
    55561 aatcttaaac tgtccaacgc atgaaagtaa tcaatttcat ttacaatcaa ttttaccttt
    55621 ctgcgctgta aattaccaat taaatgccac tttatttcag gaaaatcaaa taaatcttgt
    55681 ttcttttcta ataatttatc tactcggttt tctgcacaat tcataacgcc agctctaacc
    55741 aattcttcag cttgagcgga ttcaacagat tttgttacag ctactaatgt aacttctttc
    55801 ggatcacgat gaacaagagc acacgaatcc tgaatttctt gcttgatttt ctgcaagttg
    55861 tcagcaatca tgtgctcttc ctctccttta ttttttacga cggaaaaacg gtggcgtatt
    55921 taattcatca tcgccatgag attttgtttc ttcgcggttg aatgtatcaa attctttttt
    55981 ttcgatattg tcaaaattgg aatcatcaac tctaggacga acattttgtt cacgtctgat
    56041 atcccaatcg ccaaaactgt tttcttcttc tgaagaagtt ggtttagctt gatccatatc
    56101 taatacagtc ttttgagcag gtgattgcat ttgtgcttgt ctagctggtc ggctagactt
    56161 gcgttccttt tttgattcat cgatacctgt tgcgatgacc gtcacacgga tttcatcgcc
    56221 catttcttca ttgatagatg tacctagaat gatatttaca tcgccagttg ctgcatttgc
    56281 tacgatatct gaagcatctt gtgcttcgaa caatgtcata tccaaaccgc ctgtgatgtt
    56341 aagaagtact tgttcagctc catcaataga tgtttcaagc aatggagagg agattgcttt
    56401 tttcgttgct tctactacgc gatcttctcc actagccaca ccgatcccca tcaatgctgt
    56461 accttggttt tccatcactg ttttcacgtc agcaaagtca aggttaacgt aacctggcgc
    56521 tgtgatcaaa tcagagatac cttgtacacc ttgtcgtaaa acattatctg cttcacggaa
    56581 agcttcaagc atcggtgtct ttttatctac aacttctaat aaacggttat ttgagataat
    56641 taataatgta tccacatttt ctttcaaacg agcgatacct tctgcagcaa aacggccacg
    56701 tttcggtcct tcaaaagtga atggacgtgt cacgacacca acagtcaatg cgcccaattc
    56761 tttcgcgata cctgcaacga ttggtgcagc acctgtaccg gttcctccac ccattcctgc
    56821 agtgatgaaa atcatatctg caccgtctaa agcttcgcgt aaggattgtt cactttcttc
    56881 tgccgctttt tgtccaactt ctggctgaga accagcacct aaaccacgag tatatttggg
    56941 gcctaattgg ataactgttt cagcttttga attttttaat gcttgcacgt ctgtgttggc
    57001 tgtaatgaat tcaacacctt taacgttttc ttcaatcatt cggtttacag catttccacc
    57061 gccaccaccg actccgatga ctttgattac tgcgccgtca ttgatattat tatcaattga
    57121 gaattccata tcttattcct cccaggatga attaatcaaa aatatttgta aagaaaccct
    57181 tgattttatt ttttacattt tctccggact cttttggttc cggctcatcg tagattgttt
    57241 cttcaggtgc ttcatagctc tcatatgtgt ttgttgtttc ttgttcaact accatttgac
    57301 gagctgctga ggtttcaccc gtaacagcaa tttttgccaa ttgatacact tcactcaagt
    57361 ttgctgaata atccacgata cttattacat ttgtaaatac cgggtttctc agccccatgt
    57421 gatttggaac atacaatttt acatttacgc cgaagatttc ttgtgccaag tccacaacac
    57481 caggaagact agcagctccg cctgtcaata cgacgccacc tggcaactct aatgcttcga
    57541 tttggtccaa tgcatctttt gctttgttga aaatctgttc cattcgagct gaaataattt
    57601 ctgacaagta gcgttcatcg actttcactg gttctgactg accaatcaca tctactggaa
    57661 attcttcatc tggtgatgtc cgttctgggt atgcatcccc gtagtttatt ttcaacgctt
    57721 ctgcattgtt aaatgacgta tttaatacaa tagagatatc tttagtgaca aattcgccac
    57781 cttcttggtc aagattggta aacttcaatt gcttatcatg cataacagcc gttgttgttt
    57841 ggccgccacc catatcaatg acgatcgtac caaaatcttt ttctccgtca gaaagaattg
    57901 attcagtcag cgctaaagga gtaatcacca tttcgttgac gattaatccg gcattttcca
    57961 cgcatttacg tatgttatgg atgatcgttt taggtcctgt aaacaacaga ccgtacattt
    58021 ctaatcttac gccgatcatt ccacgaggat ctttgattcc ctcgaaccca tcgacagtaa
    58081 aatcttgtgg caatattgac acgatttgac gttcaggagg tattgaacga acaagggcag
    58141 ctgaagccac atttctgaca tcttcatcgg tgatttcctt tgaatctccg ttaactgcaa
    58201 tcattccctg gcaattttct acttcaagca tgtttgctgg caaaccgaca cttacgcctt
    58261 tgatctggat acctgctttt tcttctgctt gtcgtactgc tcgttgtatc gcttggaccg
    58321 ttttatcgat atcaacgata atgcctcggt taatcccttc tgattttgcg tttcctactc
    58381 caataatatt catttggctg tcgatatatt cagccacgac aactttaaca gatgtcgttc
    58441 caatatcaag gcctacatac attcctgttt ttgccatgaa tgggattccc tcctacttac
    58501 tattccatat acaagcgaac aactagcgtc cgttttaaaa tatacttatt aatagatata
    58561 ttcactgctt ccaattctac cataaattac tggtttttag aaagtacatt cttacttttt
    58621 aaagttgttt tatcaatttt catttaaact tgacggagtt gtacttggat tatcttctat
    58681 ttgacttgtt tcacttgaat catttgaatc agatgatccg tttgtgctag acgatccagg
    58741 tgtttgtgct ttttgcgttt ctgcctcttc acttgattcg tttgtttgat ccgcattttc
    58801 ttcactttca gttgtttctt ccgattggcc cgattcttcc gtttcttgag tgctaccgta
    58861 aggatgcgag aagataccaa cttccatgtc gatcacacct ttttcatcca tatctttggc
    58921 tacttgcgaa taatatttca tttggttcgc aagattattg atatttacaa taacttgatt
    58981 gccgtcattc atattcaatt ggatcagatt tttattgctt gctcttggcg tgtacttgat
    59041 ttcagaaatt gctttttgta attctgatga taactgattg tattgctttg ttaattcttt
    59101 gatttttgca gagtctttga actcttccaa gatagggaga ttttttgtag gatttgctac
    59161 tttttcatct aatactgttc cattttcaat cactgggtag tattgattgt tttttgcgat
    59221 caaagcaatc tctttatatt ctgttacatc caaatcgaat tcattcatcc ctttgaaatg
    59281 tatatttgct gtttcgattc ttggctgtgc ttttttaagg cgatcgatat aagtagatcg
    59341 atgaaaatac tgtccccaaa cattttctcc taatgacaat tttgtatcag agatgatttc
    59401 ttggctgttg actgtcttat tgcctgatac tgcaactgct tgtaatttgc tataaggaga
    59461 aacataatag atcaaaaaaa tcagcggtat acccaataca gtgatgatca aagctaaccg
    59521 acgaaatagt actttgtgcc gttgactttt taaattgggc agccggttca aaaaagaacc
    59581 gttatatggt ccaccgtttt tcttttgctt gatctcttca aaaaattcga tttttttctc
    59641 ggactggctt tcttcttgct gttcctcttg aaccgttttt tcttcttcag aatctgaaag
    59701 gggtgtatct aatgttatgt cttctttttt tggctcttgg tttttcatct cttgttctgc
    59761 cagatatttt cggttagcct tttgccaagg agtcaaattg ttttcatcag aagacggatg
    59821 agacaatttt tttggttctt ttttcttact cactaagacc accgccttta ttttttgatt
    59881 atttgtttta ctaagtcata aagacgtttt gaagcatcag ggattccttg ttctttagaa
    59941 gctcggcaca tttgcttttg caattcttca tcacccatga tttcgttgac tgtctgactt
    60001 agtgattcac ctgttagttc attatctgca atcatttttg ctgctcctgc gtgaacaaga
    60061 ctcattgcat ttttagtctg atgatcattc gtaacatatg ggctgggaac caacactgcc
    60121 ggtaacccca gagctgtgaa ttctgcgatc gaagttgcac cagcacgccc caccagcaga
    60181 tcactggaag ccatgacctc tgccatattt ttgatataag gctggacact aatattttgt
    60241 atttcaggat ttattttatt taggatctct tcataatatc tatctcctga agcatacaaa
    60301 acttggtaag aagaaggaat atattcactt aaaaaaactg atactgcttg attgatttta
    60361 agtgctcctt gactaccgcc aaaaatcaag actgttttct tttctggatc caaatcaaat
    60421 gttttcaaaa tatctgattt tttagtatct gctacttctt gtgctcgagg attacctaca
    60481 agaactgctt tgttttcagg aaaaaaggaa gccgcatctt taaatgaaag agcgatccga
    60541 tccacatatc ggcttaaaaa tttattggta acacctggta cactgttttg ttcatggata
    60601 actgttggaa tacccatttt ggctgcagcg tacactaccg ctccggaaac atatcctcca
    60661 gtaccgataa cgatatctgg ttgaaaatca ctgaggattt tttttgcttg acggatactc
    60721 ttaagaaaca atcggaccgt cttgatgtta tgcatggaaa tttttcgttt aaatccttga
    60781 atctccaagg tatggaaagg cattcctgta tcagggacga ttttgttttc tagacctcgt
    60841 tttgctccca catacatgaa ttcagcattc ggttcttgag tctttacata attgacaaag
    60901 gcaagagctg gataaatatg tccccctgtt cctccgcctg taaccaaaat tttcattttt
    60961 tatcgctcgc ttctactttt tctattttaa tcggttcaca gcttccatga atctgtttcc
    61021 tctagtttca aaatttgggt attgatccca gctggcattc gctggtgata ataaaactac
    61081 atcaccatct gtactgtttt ctaatgctaa aggaactgct tgttctacag aatcggctgt
    61141 tactattgtc tcgatgcctg ctttttttcc agcatccatc aacttttctt ttgtttggcc
    61201 aaatacgacc attgctttga ttccttttaa tgaaggaatt aattcatcaa atgtatttcc
    61261 tctgtctaat ccgccagcca ataacacgac cttagacgtt tcaaatccac ttagagccat
    61321 tttagtcgct aaaatattgg ttgcttttga atcattataa aattttctgc cttggatttc
    61381 tcctacatat tgcgtacgat gtggtacgcc atggaaaaaa tgcaacgttt ctcgtatagc
    61441 ttcatttgat ataccataca attttgctac agaaatagca gctagtgcat tttctacatt
    61501 atgcttacct ggtacgccaa gttcagatat ttccattatt ttttcttttt tatagtatat
    61561 tgagtaatcg tcagcataca caccgtcttc caacacttct tttgtcgaga aaggcagtac
    61621 gcgtgctttt gttctttgcg ctaattcttg tagttctgac tgattccagt taaggatcag
    61681 ataatctttt tctgtcatgt ttttttgaag gttccattta gctttgacat attcttttct
    61741 tgtaccatga taatcaatat gtgcttcata gatattggta atcaccgcta tatgagggcg
    61801 gaaatctgtg attcccatga gttggaagct agacaattcc atcacaattt tatcttctga
    61861 ttttgcttct tgcgctacac cacttgctgg atacccaata ttacccgcta aacgtgcaat
    61921 tccttgatct gctcctgcat tcagtagtag tcctgtcatc gttgttgtcg ttgtttttcc
    61981 gttcgttcct gtaatcccaa taattgggca ttccgctact tcgtaagcta gctcaacttc
    62041 cgtaattacc ggaataccca tttcttgggc ttttgctact aaaggatgac tgtagggaat
    62101 ccctggattt ttgaccatca atgaaaattc ttcatccaac aattcgattg gatgactacc
    62161 tgttattact tttatcccta aagaaagcag ctcttgtgct tccggatttt cgtcaaaagg
    62221 ttttccatca ttaacggtga caagtgcgcc caattcatgt aataatttgg ccgcacttac
    62281 tccgcttttt gcgagtccta agacaagcac tttcttattt tcatatgttg atattttttt
    62341 catttcccgt catctcctac caaacgatcc ataatgtgat caacgaagtc acaatgctga
    62401 tcaaccaaaa aactgtatca atcttccact ccgaccatcc gcacatttca aagtgatgat
    62461 gaattggaga cattttaaaa attcgttttc ctgtaagctt aaaggaagta acttgaagca
    62521 tcacacttgc tgtttccata acatagatca aaccaatcaa aagcaatgtc cattcttggt
    62581 ttagcattat agaaattgcc gctaaaagtc cgcccaacgc taatgaaccg acatctccca
    62641 taaaaatctt ggcaggtttt ctgttgtaga cgaagaaacc cagtaaacct cctaaaacac
    62701 ttaagcaaat gattaataca tcatactgat gctgatgcca agcaattaga ccgtaagctg
    62761 caaaagagat acttcctaag ccagcaacta atccatcgat gccatctgtc agatttactg
    62821 catttgaaaa tccaacaagc cagaagagag cgaaaatacc ataaatgatt cctaaaggta
    62881 gatctagtcc gaagaaattc aatacacctg gataaccttc agaacggtaa acaagataaa
    62941 agatgattcc ccctagaatc tgtccaatca atttctgctt cgagttcagt cccatgtttc
    63001 ttttcttgaa tatttttata aaatcatcaa gaaagccgat tactccgtat aacgctaata
    63061 caaaaagcaa gatgaaaaga gtaggagtta gctgtttttg ccaagctcca acccaaattc
    63121 ctgtcaaaat agagccgatc agaaacacta agccgcccat tgtaggagtt ccggctttgc
    63181 tattgtgcca ctttggtccc tcctcacgaa tcgcctgacc ttgctttttc atttgaaaat
    63241 aaccaataaa tagcggcatt gcggcaattg tcattgcaca actgctgaca atgggaatca
    63301 atgcttgtgt ccattccatg ctcttttctc tcctatttct cttcttattc ttctaaagta
    63361 aaactaagtt tgtcttcagt gattttttca tatggagcaa gttcttgttt gacacaataa
    63421 ccatctccat caaaactaac ttcaataccg aggattttcc ccagcttcat gatatctgct
    63481 tttgaccatc ctgttacgtc aggcatcagc ttatcaccac cagtatatag gatcagtttt
    63541 tctccagaga tcaactgatc accgttggca gtagattgtt tttgtacttt tttaccatta
    63601 ccgatcacta caggttgcag tccacttttt tgagcatcag ctgctgcaac gtccgcttca
    63661 agattacgat aatcggctac ggatactttt tctgtttttg tttctgtatc tgaatcttct
    63721 tcagtttctt taaaatccat tgcccgtttc atcaatggat ttgcgatttt agccaatgcc
    63781 attcgatcat atgtcttagg gtgtttcatt gttagataca agacatagtc tggatcttca
    63841 gaaggaacca tctccacaat cgaatacagg taggctgtat ctcctgtttg atatccccct
    63901 gtatcagaag cgatttgggc tgttccgttt tttgccgata cattgtatcc aggtacacta
    63961 tatacaccat aagcactacc gtagttttcg ctttcgacaa catctctcat atattcacgg
    64021 actttttctg ttgtttcttt tgaaaaaggt tgccctagaa cttctgtttg agttgttcgc
    64081 tcttctccag tttggggatc caccactttg ctgatatatc gaggttgaat cattgtaccg
    64141 ttattcgcaa tagaagtgaa agctttcatc atttggaaat tcgtcacacc taccgcttgt
    64201 ccataagcac tcattgctcg gtcgacaata tttaatgtag gtaaagcacc gtttacttca
    64261 tcatctaatc cagaatgagt actttgtcca aatcccagtt tttgtaaata gttataccat
    64321 cgtccgccta atcgctgttc cagtatgacc atcccgacgt tacttgacca tgaaagagct
    64381 tgacgcatgg tcagcacacc tttttcacca aagtcatggt cattgatcgt agcatcttct
    64441 acttggattt ttccagattg gaaggtttca ttctcgttga attctccttc ttcgatagca
    64501 gctgcagtag tgaaaacttt catggttgat cctggttcat aactatcttg taccaagaaa
    64561 ttacgccaga tagcatcttt tccagtcaat ccttccattg tttcaggatt gaaagtaggt
    64621 cgttgtccca ttgccaagat ttctccggtt tttgctttca tcaggactgc tgtcaattct
    64681 tctggctgat attcttcatt tacttgatcc atcagtgttt ctaaataact ttgtagtctg
    64741 ctgtctaaag tcgtgtagat atcttgtccg tcgacagctt tttcttcttc tgccactgta
    64801 cctggcagag gattttgaaa attatctttt tgataaataa tcttaccatc tttaccgctc
    64861 aagatatcat tgtaagcaga ctccaaacct agtttaccaa ctaagccatt ctcatctttg
    64921 tctggaactg cgtatccaat aaaatgagaa gaaaagacgc catttggata catccgagcc
    64981 tgatggtcga caaaataaag accagcgaca ccttcttttt tcatgtccgc ttcgatgttt
    65041 tgcttggttt cttgggagat atttttccca tagctaccaa attcaacttg atacagctct
    65101 ttttttgcgc cgtcctctaa aatattcagt gcatcttttt tgtctataga aagattctta
    65161 tgcaggattt ctgctatttt atcaaaattc ttttcttcaa catacagttt tttatcccca
    65221 gaggtatatg tttttgagag tatcgcttta attgaataag aggaggcatc ttctgcgagc
    65281 gccacaccgt tacgatcata aatcgttcct ctttttgctt ttactacttc acttccttgg
    65341 tacaactgtt ttgttttttc agctaatgag gtaccagcaa catgacctcc aataacgata
    65401 tacgagaaac ggacggcaaa taaaaagaac aatccaatac tcgtagcaaa taaaatgata
    65461 cctacttttt tgcgattgtt catcggattt agatttttct tttccataaa ccgccgaatt
    65521 ttatttttaa gactcattta cttcactttc cttaaattgt cgtcattaat agacaatcct
    65581 ttttcttctg cgatttgttt aatccgttca gtacgagaaa gttcgttttt ttcttgttct
    65641 aaactcgttt tttcttgctt tttatcattg atttctgttt gtattgacgt gatttctttt
    65701 tcagctccgc tgatatttgt gcgcagcata actgttaaaa cacataaagc aacaatagca
    65761 atcgccacgc ttgccacagc aattttttcc aaagcagaaa tctgctttaa tcgttttgcc
    65821 ggtgattgtg gaagaatgat ttcttgattc gaagaagaat caggcatttg ttgttccggc
    65881 tgatccacca attcaggtaa atcatattcg taatcttgca ttttctttaa ttctgccatc
    65941 attctactcc tctttcattc ttattttttc tgcaatacgt aactttgcac tgcgtgatcg
    66001 attgttttct gttagctctt cctctgaagg aaggattggt ttacgagtaa tgactttcag
    66061 ttctggttgg aattcgtcag gtatgacagg aagtcccggt ggtagatctt gtaccgtact
    66121 atattcttta aacatacttt tgacaattct gtcttccaat gaatggaatg tgattacgct
    66181 gattcttcca ttgactttca ataaccggat tgcctgttct aaagaggcct cttcggcacc
    66241 gagttcgtca tttactgcaa tccgcacagc ttgaaaaatt ctttttgcag gatgcccgcc
    66301 ttttcttctt gcgggagctg ggatgacttg tttaatgatt tcgacaagct caccagttgt
    66361 ttggattggt tttgtttttc gtactcgttc gatttcacga gcaatttgtt tggaaaattt
    66421 ctcctcgcca tagcggaaga aaatctttac taattcatgg taggaatatt cattaattac
    66481 gtgataagcg gataaaggag ctgactgatc catgcgcata tccaacggtg catcttgatg
    66541 ataactaaat ccacgttctg cttcatctag ctgaggagag gaaaccccta gatcatacaa
    66601 aatgccgtct accttatcta catgctcact caagcgttct tctaattctc tgaagttcgc
    66661 tttgataaat gtgaccatac cttgttcgac ataagaagct aaacgcagct tggcgtgatc
    66721 gattgctttt tgatcttggt caaacgcata cagatgccct ttagaactta gttggcttaa
    66781 taaatattca ctatgtccgg caccgccgag tgtacagtct acataaacgc catccggttt
    66841 gatgttcaat ccatctaccg tttctttcaa catgactgta tagtgttgaa atgtttcagt
    66901 cattagtcag cctcttccta ttcttatagt ccgaaatcaa tcatactttc agcaatttcg
    66961 tcaaagttct cttctgctgc tgcagaaaaa tcttgccatc gtgtctcatc ccaaatttca
    67021 attcggttag atacaccgat gactacgcaa ccttttgtca aagctgcata atttcttaac
    67081 gtgctgggta tattgattcg tccttgttta tcaagttcgc attcagtggc agccgaatag
    67141 aagaatcgca caaaagtacg tgcgtctttt ttagccaacg gcatttcgtt cagtttagct
    67201 tccagatttt cccattcgct catcggatag ccgaaaagac agccatccaa tccgcgtgtc
    67261 agaacaaact tttcgccaag ttcttcacga agttttgagg gcacgattaa acgtcctttt
    67321 gcatcaatat tatgttggaa ttcacccatc aacattgaaa gccccccatt cctccactaa
    67381 ctaaaattag tctaccacaa tcccccactt tctaccacct tttctgggaa atccgggaac
    67441 aacttttttt attcataaac atttttttaa aaatcaagct gcaaaaagcc ttttaaaaga
    67501 ctttttacag cttttctatt tttttcatag gtaagaaagt atgttcacaa tgatcaagac
    67561 agcgtacaag acgattgtca acagaaaaac caaacgccaa gtcatcttga aataacgcgg
    67621 ataaataatt tcttcataat aatatgcttg gaaaacagca atacaaattc ccattagcaa
    67681 aacagaaata aagaaaaacg gtaagataga aattgtccct gtatttctag acaatgcatg
    67741 cataccgccc cacaaaaaag gaatcgccaa atcaggggct tttattttaa aacgacgatt
    67801 taacgaaaat aattttacaa gaaattggca accaaacaaa acgattaccg ggtaaatata
    67861 ccaaaacaag ataagtggtg tgaatgtgcc catgaacgta aactcctttt ttatcgttgc
    67921 ttcaagtata ctgtatatac tttacacaac tgtttcattt tatttaaatt tatgcagtta
    67981 tattctatat cttacaaaca cattccatgt attccaattc cctatccgtt ataataaaag
    68041 aaacaggcaa caggaggaaa atcatgatca aatattttac attagataat gagaaactgc
    68101 gtaataccgg aaaggaaact gaccaaacga tctggtattg tgtagaacgt cctaatcaag
    68161 aagagattca tcagctgaca catcaatttg aaattccgtc agattacatc acttccatat
    68221 tggacgatgc agaaaattcc cgtacagaag aattcaatca atcaaagttg acaaagccag
    68281 cgttattatt gttacaatat ccttttgcta aaacaagtcc tagcggttat tttcaagtgg
    68341 atacataccc gctctctatt attttgacgc ccaaaaagaa gcttatcaca attacgaatc
    68401 atgaaccact gtttttaaag aaagtcttta gccagacctt tccaaaaagt gatatttcgc
    68461 ctaatctcaa tatcttcttt caattcattt ggcaggtcac actctcttac aacagttatt
    68521 tagctacgct tcaatctcaa tgcgatactt tggaagccaa acttcaagta tccactgaaa
    68581 acaaacaact ttatcaaatc atggatatcc aaaaaagcct cgtttatttc agagaagcga
    68641 cttctgctaa tttagatgca ttgaaagaac tagcgcaaaa taaacttttc caagaaaatc
    68701 atgctttgaa aaatcattta cgtgatgtat tggtggaaac agagcaggca atgactactt
    68761 ctcgcattca attaaaacta gtagaacaaa tgaatcagac tttttcagca atcgtatcga
    68821 ataatctgaa taatattatg aaaattttaa cttctttaac gattatttta actataccga
    68881 caatcgtcgg cagtatctat ggaatgaaca ttaagttacc tattgctgaa agggatgatg
    68941 cctttatatg gctagttcta ttaacattat ttatttgtat cgtaactacc tattatttaa
    69001 aaaaagggaa gtttttataa aaaagccctc ttgtggaaat ggaaaaaaga ttgtacactt
    69061 gaatcaacta attaatgacg aggttgaaaa caatgcgtga aaaaaaacaa acaagtactt
    69121 ttgctaaagt aacaaaattt gtcatttgga caatgctgat tttaacgatt ggatctgttg
    69181 tattgactgc tgtaatgagt gtcatgtaaa aataaaatat agctatcagc tgctgaaaag
    69241 gacctgaacc actgcgaggt ggctcaggtc ctttttgcga gtttcaatag ttcttttgca
    69301 tgttcgagtg tgagttttgt aatctcactt ccagccagca tacgtgcaat ttcttcttct
    69361 ctttcttttg ttttcaactc tgaaacagaa gtttgcgtac gtccatcaat cactgctttc
    69421 acaatataat attgatagtc ggcgactgca gcaacttgag gcagatgagt gatgcacaaa
    69481 acttgagagt atttcgatat ttgagaaatt ttatctgcga tggcctgtgc aactcttccg
    69541 ctcacacctg tatccacttc atcaaaaata atacttgtca caccttgctc agaagaaaaa
    69601 atagttttca aagcaagcaa catccgagac agttcgccac cagaagcgac tttcaccaaa
    69661 ggcttcagtg gctctcctgg attggtcgta atgtaaaatt cgatttggtc aaatccatca
    69721 tgattcaatt gtctattttc ttcttttaag aaacgtacct caaactcagt gttttccata
    69781 tacagacttt tcaattcatg cagaatggat ttttccaatt cttttgcgat ttttttacgc
    69841 gcttcatgaa gatctgcggc ttgttgctga agtagaagct ctttttgctc tagttcctct
    69901 ttcattttgt ccaattgacc ttctgaaaaa tcagaagaag ctagctcttc ggtgatttcg
    69961 tcatagtaag ccaatattga ctcaatggat tcgccgtatt tacgtttcaa ctgtcggatt
    70021 aattctaaac gttgcgtaac ttcttctaaa cgactctcat ctaactccaa taaatcaatc
    70081 tgacgactca tatcgccgat tgcatcttgc agcaaatagt aagcactttg gatattttca
    70141 taaattgctt catactccac atctagatgg gcgatccctt gcagttctga aacggccaag
    70201 ccaacaccat ctacactgtt ttgttcatta ttcgtcagtg ctccgtaacc tgcagctaat
    70261 ccatcagcaa ttttttgata attgctaagt ttttctcttt cttcttttaa tttctcttct
    70321 tctccgactt gcaaatcagc agcagctatt tcttcttgct ggaaatggag catatctatt
    70381 ctctgaacat aggatttttc attttgctgc acttttcgta cccttttttc cagctcacga
    70441 taagagatat attcttgctg ataactcttt ttcttctgtt ggaaagctac atctccaaaa
    70501 cgatccaata aagcaagatg tgattcaggc tggagcagtt cctgatgttc gttttgtcct
    70561 tggatatcta ctaaataact gccgattctt ctcagattag ctaatgtgat aatatgacca
    70621 ttcactcgac aaacattttt tccagttaag gacatatccc gtcgaacaat cagattgtcc
    70681 tcatctgttt cgatccctaa ttcaaccatc agttcagaga atccttcttg ttttggtagt
    70741 tcaaacaacc cttcaagcac acatttttct gctccttgac gtatataatc gcttgaacct
    70801 cttcctcctg ctaacagacc taaagcatcg ataataatgg atttacccgc accagtctct
    70861 ccggtcagcg cggtcattcc ttcgtgaaaa gaaagccgca attccggaat aatcgcaaag
    70921 ttagtaatac tgatttcttg tagcatcaca ttcgctcctc aacttaaagg taatgcagga
    70981 agtctttctt caataaaact gcctggtctt cagttcgtgt aatcatcaaa acattatcgt
    71041 catcattgat aatcgaaaat aattctttct tgtaacgttt atcaatcaaa tttgctgctg
    71101 ccgaagcatt tcccggcaat gttttcaaaa taacaaattt ttccatttgg tcaactgcca
    71161 cgaaagcgtc ttttaatagt ttttccaatt tagtatttac atcttcgttt gtttcagctg
    71221 gtatgctata acggtagccg ccatctgaag ccggtacctt gatcagtttc agttccttta
    71281 tatctctgga aatcgtagct tgagtaacag atattccttt gcttttcagg atttcaacaa
    71341 attcttcctg ctttcggata tcttgctcac tcaacaatcg agtaatcaaa cgatgccgat
    71401 ctttttttct cattacgtct tcgctcctta gcatattctt cttaatcaat gatagcttat
    71461 ttccttggta taataaagaa gaatccgcat acttctcact tgattttcac agttacaatt
    71521 tatttatggt caaactgttc gtgtgcattt gaaaccactt ctgccatatt aatagctgaa
    71581 ttgattgttc cgctttcggg tactttcctc aagtgagcca agaactcgat atttccttct
    71641 ccccccgtga tcggagaata acttaattcc agcacatcat atccaccttg tgccgcaaat
    71701 gacaaaatgt cttctaaaac tttttgatgg gtctgagggt cacgaacaat tccgtttttc
    71761 cctacagctt ctctgcctgc ttcgaattgg ggtttgatta aagcaatcac ttctccctct
    71821 tcttttaaaa tagcatgtaa tggaggcagc atcagtttta gtgaaataaa agaaacatcg
    71881 ataacagcaa cttctggaat tcctaaggta aagtcttcgg gttttgcata acgaaaattc
    71941 actctttcca ttacgacgac tctaggatct tgtcttattt tccaagctag ctgattgtag
    72001 ccaacgtcta aagcataact catttttgcg ccattttgca aagctgcatc agtaaatccg
    72061 ccagtggatg caccaatatc taacaatgtt tttccttcaa cagatagatc aaatgctttg
    72121 agtgcttttt ctaattttaa tccccctcga gaaacataag gaagtttttt acctttgact
    72181 cttaattcac tactcactgg aattttttct cccggtttat caaaccgctc gtttttttcg
    72241 ttgtaaatca aaccagccat cactgaacgt ttggcttgtt ctcttgtttc aaataatcct
    72301 tgtttgaccg ctaatacatc tacgcgttct ttttccattc tttccctcat ttctccacat
    72361 caaattgctt gatcatctgg cgcatcagcc cgccatcaaa agaaaaaacg tcctcttcta
    72421 atttgtctat gatcttgttg gcggataaca gctcaatctc caacgcctca cgtgtctttt
    72481 ctaggcctaa taaacgcggg taagtatttt tttccatacg ttcatcttta ccagctgttt
    72541 tccctaatgt tttcgttgta ccaatcacat cgagtagatc atctcttatc tgaaaagcta
    72601 aacctaaatg ttcggctaat cgttgaagta gcaataagat ttcttcaggt tgcttcgcta
    72661 atatgccacc agccagcaag gcataacgaa tcaaatgccc tgtctttctt tcatgaataa
    72721 atgctaattc ttctaaagaa agctttttcg attcgccctc gatatctgca gcttgcccag
    72781 caaccatacc ttgtgatccc gcacaaacag ctaattgctg aagcagcaga agcttaggag
    72841 aattacctaa gtgtgccata ctgattagct ggaatgctcc tgtaagcaaa ccatctccgg
    72901 ccaatatagc taatgcttcg ccgaatactt tatgattggt aggttttcct ctacgaagat
    72961 catcattgtc catcgcaggc aaatcatcgt ggatcaagga atacgtatgc accatttcta
    73021 aagcagctgc tacttgatag acgggaacat caataggctc atcaaaggaa gcaacggccg
    73081 ttaaaagtaa taagggacgt attcttttcc cgcctgcttt cactgaataa ctcatcgcat
    73141 catagagtcc tctatccgtt gtatgctcat ctaaaaaaga caagatttct ttttctacag
    73201 caggtagatg aatagctgaa aagtcagtta atctcatctc agctttcctc gtttccgtca
    73261 aaatcaactt cttcatttga ttctgtcatc attttcgtca aggtctcttc tgctttagct
    73321 aatgtatctt ggcaatgttt gcttaattcc attcctcgtt taaacgaatc tagagctgat
    73381 tctaatggaa cgtctccttg ttctaactgc atgacaattt tttctaattc ttgcagtgac
    73441 tcttcaaaag ttggattttc catattttta ttctcctttg tctatctctt gtacttgtcc
    73501 tttaacgatt ccatcttgat aatggatcgt cagcaaatcc ccttgatgaa tctcggtgac
    73561 actttttact acttgatcat ttaacgtcgt atagctgtag cctcttccca tgattttcaa
    73621 tggactcaac aaatcaagag attgaacagc ttgagcaaaa cgctgctgct gttgtttcat
    73681 atattgttgg attttctctt ctaagcgttt ttctagataa gcagtttctt ggcgagctgc
    73741 tttaactcga taaagcggag ccacttgttc caattggtgg cttaatgcga tcgtttgttt
    73801 ttctttcgca tgataaattt gctgtgtagc ttgatataat cgctgtttca actggtctaa
    73861 tttgatggtc tgaccttcat acaatctttc aggctgccga aatacatagg aacgtcgagc
    73921 acgctcgaac cgttcttgtt tgcgctggat ctggcgtaca aacgcctgct gcaatctagc
    73981 ttgccgttct tgaatgcgca tcaactcttc tgacaaaaca ggaacagcta attctgcagc
    74041 tgctgttgga gtggcagccc gaacatctgc tactaaatca gcgatcgtgg tatctgtttc
    74101 atgtcctaca gatgaaatca ccggcgtatt agcttgataa atcgcccgag caacccgctc
    74161 ttcattaaac ggccataaat cctcaatcga accaccccca cgaccgatga tcattgtatc
    74221 gaaattgccc atctcttcta ctctctggat attcctgaca acattgtccg ctgcttggtt
    74281 tccttgtaca actgttggga acaaaaccaa ttggacaatt ggatatcttc ttttcaccgt
    74341 cgtgatgata tcgcggataa ccgctccgct tggactggta aggaccgcga ttcgctttgg
    74401 gaaacgtggc agcatttttt taggcccttg aaaaaggcct tccttcatca gcttttcttt
    74461 tcgttcttcc aattcttgat ataaagcacc tattccatca ggctccatgt gctctacata
    74521 gatttgataa gaacctcccg cttcatataa tgaaatacgc ccaacgacaa ggactttcat
    74581 tccttctttt ggctggaatc gtaatttttg gaatgctcct ttgaacataa tagcagatat
    74641 ttttgcatgg tcatctttta agctgaaata ttgatgggca ttggcacgta aacggaaatt
    74701 cgatatttcg cctgttaaat atacgcgttc taaataagga tcggcatcaa attttctttt
    74761 caaatacttc gttaatgtag tgacagttaa atattcttgt gccatctatt cacctgctcc
    74821 aaaagcttcg gcagatttta cggtttgata catcagcatc gtgatagtca ttggccctac
    74881 accctttggc acaggagtga tccagcctgc aataggctcc acttcatcaa atttcacatc
    74941 accgatcagc ttaccgttag catcacgatt catccctaca tctatcacta cagcacctgg
    75001 ttttacgaat tcttttgtga caaaatgtcc acgtcctatt gctacgacta aaatatctgc
    75061 ttcttgtgcc agttcagcca gattttcggt tttagaatga gcaacagtaa cagttgcatc
    75121 tgccatcaag agtagctgag ccatcggttt accgacgata ttgcttcgtc cgataactac
    75181 tgctcgttta cccgccggat cgatagaata agcttcaaac attttcatga tgccgtatgg
    75241 agtacaagga atcttgtccg gtttacccgc aaggagtctg cctaaattca tcggatgaaa
    75301 accgtctacg tctttttcag ggcggatagc taacaggatc ttttcttcat ctatatgctt
    75361 tggcaatggt aattggacca atataccatg aaaactagga tcttgattgt atttttcaat
    75421 ttctgcaagt agttcgtctt cagaaatcgt ctcaggataa cgctctacca atgaatggat
    75481 tccgatttct tttgctacac gttctttatt tcttacatag atttggctgg ctggattttc
    75541 ccctaccaat aaaacgacta atccgggatg gataccatta tctttcaatt tttggatttt
    75601 ttcagcaatc tctgcctgca ttttttcagc taattctttt ccgttaatca attctgccat
    75661 ctaaccactc ccagatattt tttgtgtttt cattctacca tagaattttc ccgatttata
    75721 acaggtttat gatgattcat agaaaaaagc taggacttac gccccagctt ctaattcttt
    75781 caatacattg gataaaacac cgttgacgaa tttacgtgaa cggtcatcac taaatgtttt
    75841 tgccaactcg attgcttcat ttaaagcaac agccgctggt acatctgtca cataagtcat
    75901 ttcaaaaatc gcgatacgca agatcaccaa atccattttt gagatccgat tgatgtgcca
    75961 attgtttttt aaatgttttt caatcacttt atccaattca tctttttttg cacagacacc
    76021 gccaactagc gtatcaagat aaacaggaac aaactctgat tcatcttcgt tgatcatgtc
    76081 gcggtggtct aattcgattg cgttgaaaat cgcatcttct tttgttaggt ctgcattgaa
    76141 gtctaatgga aataaagcct gcaatgccat ttcccgaatc tcgtgtcttg ataattcttt
    76201 attcattctc ttcctcttcg tcttcaaaaa gttcttcgat tgttggttgt tctaattttt
    76261 caggaacaac agctactaca tggatattga cttcagataa atcgatatcc gtcatgaaaa
    76321 ggacttgctg gctgacccgt tcctgcattt ccattgctac tttaggaact gaaacaccat
    76381 attctagata acagtaaaga tctactttca agccatcttc ctcgtttgct aagtaaacac
    76441 cttttccgtg agctgcacgt cctaataatt cagtgacatt gctggcaaaa gttccgcgca
    76501 ttccgtatac gccttctact ttagaagctg caatcccgat aattacttcg atgacttcag
    76561 gagcgatcac gatctctcct aaatcttggt ttgcgtttaa aatcaaattt ttttcatctg
    76621 ccatttattt attcctccct ttgcctaagc acgagataca tccgaattct ctatgatgcg
    76681 gcatagtgat cacgtggtaa gaaaactgcc gccgatcagc tgcaaactgc tatgaattgg
    76741 agaaaacacc caactttatt atctcttaca gcttaaaagt tcttctatag tgtatcatta
    76801 aatcgagtga ttttctataa caaagtctgt gaacactgat ttttacgctt acttcttcta
    76861 ttgatgaaac tataagatga tcaattcttt tggtgaacga gtcaacacac gatttccatc
    76921 ttctgtgatc aacaagtcat cctcaatacg tactccaccg attcctggta agtagatccc
    76981 tggttcatca gtgatcacat ttcctggtac aaattgcttt tctgcacgag aagaaacgtt
    77041 tggtccttcg tggatctcta aaccgatccc atgtcctgta gaatgtccaa atgcttcccc
    77101 gtaaccatgt tttgaaatat aatctctagc aactgcatct aattggacac cagtcattcc
    77161 tggttttgca gcatcaataa ctgctaattg agcatctaag actatttgat aaatttcttt
    77221 taatttttcc ccaggatcac caatagcgaa tgtacgtgtc atatcggaaa cgtacccttc
    77281 gtaatagcaa ccaaagtcca atgtgatcaa gtcaccttgt tcgatgattt ttttgcttgc
    77341 tacaccatgc ggcatagccg aacgcaatcc gctagcaacg atcgtatcaa aagaaacacc
    77401 tgaagcgcca agagaacgca tataaaagtc tagttgattt gcgacttcaa tctcagtcat
    77461 gcccggttgg atcattttca ggatgtgatc atatgccata tccgcaatat gacatgcttt
    77521 ttcgataata gcgatttctt cttcatcttt cacttctctg agttcttcga tcatctgtga
    77581 aacaggtact aattccgcat cgatgatttc ttctaaaacc gaatattcta aaaatgagac
    77641 tgttgtttcc tcaaaagcta atgagtgcag ttcttctttg cgtactaagt ttgctacctc
    77701 atcaaaaata ggtccgacat ttttaataat ttcaaaccct tgagcctgcg ctgccgcctg
    77761 ctcggtataa cgaaaatcag taataaaaaa tgctttttcc aaagtaataa cagctaatcc
    77821 tgtcgtacct gtaaagttcg ttaagtaacg aagattgtac ggactggtaa ccaaaaatga
    77881 atccaaattt tcttcttgca tttttttgcg caatttttct acacgtagca tcataatgaa
    77941 actccctctt tctttgcacg cttttttcca ttatagcaaa cttttcttcg tacacgacag
    78001 ttaaataaaa atttccggaa aacttctttt tattttaaat aaataaagcc aaaaaggagt
    78061 cacagtctcg ctgcaactcc ttttagttta ctattttgta aaaacttatg atcgttttcc
    78121 aaagaaaaat gagacaactg ctaccaaaat cactgcgcct aaaatagatg gaataagtgc
    78181 catacctgca gcttttggtc cccattcgcc gaaaatcgct tgtccaatac ttgaaccgac
    78241 tagtccagca atgatattcg ttacacagcc catcgaattt cctttactgg tgatcgcacc
    78301 tgcaattgct ccgatcacgg ctccaacaat caatgtccat aaccaactca taatataacc
    78361 tccttctttg ctaatcaatt ttagcatatg ggatttaaag gggacaaata attggttttg
    78421 ctatttcata ttttcttctg caaagtaaaa aaataaagaa aacctcctct tagaagtttt
    78481 ctttattttt tacttgcgtt taaacatact acgaatacca cgtttcacta catcaaagaa
    78541 actcaatgat tgtaccattc tatcttcatc tacatattct ctgatcgcac gaagcacttt
    78601 tttaggttct ttggaagaaa atgtgtatgt accatttttt ttcgtttgaa tggcatatcg
    78661 aggaatccat tttcccttta gcatgactga tgctagtaca taatccactt cttcccatgg
    78721 aatttggata tatttgcgtc tatctcgcgc atggtaaaac tcaaaaccat tatcacctat
    78781 catgatttgc ccgtaatctg tcagacccgt aaacgaagta gcatcaatta ccaagtctac
    78841 ttttgtattg attgattgaa ccattttctc cctccagata tttgtttatg aacagtatac
    78901 tatataaaaa aagctcggga caacgcccga gctatatttt tatggattat aaaagtccga
    78961 tgacgtgtcc aacaacaccg actacgaata aaccaaggat gataacgatt ggagaaacct
    79021 ttttcttaag caaccacata cataacaatg ttaatcctaa tgcagctaaa ccaggaatca
    79081 attggtctaa gttgtcttgt aatgttgtta ccttttcagg agaaagggct aagccattat
    79141 taacttgttc aaatgcactc ttgattcctt caccacctga aggcaattta tcccattcga
    79201 tgtaagcacc tttatctaat tttacagttg atacaactgg taagaatttg attgataccc
    79261 atctttgtac aagggcagct aatacgaaca tccctaaaat tgaagcacct tttgttacgt
    79321 cttgtaacaa tccgccagaa aggtcatcag taattttaga accagctttg tagccaaatt
    79381 cttgtgtata ccacatgaat gcccaacgaa taatgttcca acctaagaag aagatgattg
    79441 gtccaaggat atttcctccc atagctaatg aagcacctag tgcacctagc attggacgta
    79501 ctgtaaacca gaacactggg tcaccaacac cagctaaagg acccatcata cctactttaa
    79561 ccccttgaat cgctacatca tcaacaggcg cgccattcgc acgttcttct tccagagcca
    79621 atgtaacccc taaaattgga gaagcaatgt atgggtgtgt attaaagaat tccaagtgac
    79681 gttttaatgc tgcagaacgt tcttcttttg ttttgtataa ttttttgatt gctggaatca
    79741 tagaaaatgc ccagccaccg ttttgcatac gttcgtagtt ccatgaacct tgaatgaagg
    79801 tagagcgcca cgcaacagct aaacgatctt tttttgataa tttgattttt tcttctgcca
    79861 ttttattttc ctccttcaag attaatagtc attcaaaatg tcgcctaaag ggtcaccaga
    79921 gttgcttccg cctccattat ttgatgaacc acccatttta gaaaggttca agtaaataag
    79981 agctaatgca acacctaaag cacctagagc aatcaatgtt aattgagaaa ttgcagctac
    80041 aacgaaacca ataacgaaga atggccatac ttcttttgta gccatcatgt tgattaccaa
    80101 agcgtaacct acagctacga ccattccccc accgatagcc ataccatctg ttaaccatgc
    80161 tggcattgat tcaaggaatg attgcacagt ttgtgctggg ataaatagca acgctgctgc
    80221 tgggaccgcg atacgaaccc cttgtaaaca tacagcaatg atgtgccaca tttcaacctt
    80281 acggatgttt ccttcttcag cagccgcatc catcaaatga acgattggaa cagcgattgt
    80341 acgtacgatc attgttaaga acaaccctgc aacagccaaa ggaacggcaa tcgcgatcgc
    80401 agaaggaaca cctttaacac cttgtccacc taaaactaaa ataattgctg atgctactga
    80461 tgctaatgca gcatctggtg ctacagcggc accgatattt gcccaaccta aagcgatcat
    80521 ctgaagcgta ccgccaagta cgataccagc ttcgaggtta ccggtaacta aaccgatcaa
    80581 tgtacatgcc actagtggtt gatgaaattg aaattcatca agaatacctt ccataccagc
    80641 tagaaaggca actaatacga ctaaaattat tgaaataata gacatgataa gcctcctata
    80701 aattttaata ttatttatta cgcttttgct aattcagctt ttgctttttt caaaatatca
    80761 tccatgttat cgcgtgaatc attaggtact ttacgtacat cgaatttcac acctttttgt
    80821 tcaagcttct caaacgcttc aacgtcttct tgtcccatag acaatacttt acttacaacg
    80881 actttaccga ccgaatgcgc cattgaccca acatttaatt ctttgatgtc tacgccacct
    80941 tcaacagctg tcaatacgtc ttcaggattt tcaaataaaa gcaatgcttt ggtatttcca
    81001 aaacgagggt cttttgctac ttcgatcatt ttgctaatag gaataacatt tgcttttaca
    81061 cctggaggag cagcttgttc gattagtctc ttacgcagat catcctttga tactgcatct
    81121 gaaacaacga taatacggtt tggttgcact gcttttgtcc aagctgtcgc aacttgtcca
    81181 tgtaacagac gtgagtcaac acgtgctaat acatatttga tttttccgtc tccgaccaca
    81241 gttccttctg gaagtgctcc tttaggttgt gcgtcttcaa ctgctgcttt cggtgcttct
    81301 gccggttcta attcttccgg ttttactttt acaccgtctt ttgctgtttc aatgatgtgt
    81361 gctgcaattt catgagcaga ttccattgaa aagcgtgatg cgtaggcttc aatcaacatt
    81421 ggtaaattca aaccgctgac gattgcccat ttatctttgt gctcttcgaa taatgtgttt
    81481 gattgattga acggtgttcc gccccataaa tcaactaaga ataacacttc gtcttgatta
    81541 tcaaaagaag caaccgcttc ttccaatttg gctctcaagt catctggacc ttcacttggt
    81601 ttcaaaatca cggctttgac gttttcttgt tcgccaaaga tcattgaacc ggattgtaag
    81661 attccttcag caaattgtcc atgacttgct aggataatcc ctaccatatt tatacctcct
    81721 atttactttt gttgtaacaa attcaaaaat attaataatt gctaaccatt acaacagaat
    81781 tatttgacct tttccaacaa ccattcttgc tacacctttt tgattttcgc tagtagttcc
    81841 atcaaaagga ttttcctgtc agcaggtact tttcgaatct ctaactccac ttcttgactg
    81901 ttcaagtaac gaaaggaatc aatatcttgt tgatttactg aaacaaaatt ggtaatcatt
    81961 gatttgcctt cagtaaaact cataccgccg atattcaacg acttcaactg tacaccgcag
    82021 cggacaaccg cttcaacatc ttggggattg gtgaataaaa gcatcacttt tgctcgtgat
    82081 aataatccac tatgatagat ttgaatcatt ttctgcacgg ttattacatt tgctgtgatc
    82141 cccggcggtg cagcttcttt tagtagaaac tttctcaatt tatcttgagc gactacatcg
    82201 ctgatcacaa tgatacgttc aacacctgtt tgcttcgacc aaactgttgc gacttgcccg
    82261 tggattaaac gatcatctat acgtgccaat cgaatatcca tctgcaaaac tccttcttcc
    82321 tcactacttc acagaaaccc ctatcatttc tgtgtgaaca accacctcct agcacacact
    82381 actataagca aaaagcgtgc caactttttt acgatacact aattgaattt aaaggattct
    82441 taaccaatac acaaaaacga tacactataa aagtgtatcg tttttgtgta ttcatggcac
    82501 agtgtattct tttttatcta actgattatg gactagacgt agcagataat acatttcttc
    82561 tatcggcact tttactttta tctgatcttc caacagaaca actgaggctg ccaattggct
    82621 ataaagagga tcattcgttg tatttgtcaa ttcgttttct tccacagtca atggttcatt
    82681 ccgaataata cgttcgatca ttcctgctgt gtgcatcact aggttgattt tgaatccata
    82741 ttcttcatcg ccgattcccg tcgtctgaga gatttgtgta gaaaattgcc acattggatc
    82801 gattaactta ctaccattaa taaagatgaa gttatcagag ataaactcaa cacacgtatt
    82861 tttagctgac tcttcatcta gggagatgaa ttcagtttcc tccaattctg attccatcag
    82921 caactgatcc aaaatgactt cgatattttg atcaataaaa cgttctaaag gaataaatgg
    82981 ggcttggata ttcaaatcag taatcccagt agtagctatg atctgatatt ctttttggat
    83041 aactggcagt tcttctttca cttccacaat cgacaaagct agaacttcta attctgtttc
    83101 ttgacgcttg tttaccgctc gttcgatcaa ttctttgatt ctttgtgctg taccttcgcc
    83161 agatgcgcag atagccaaaa cagctttttt cttatgaata ggttttgctt cctgaacaga
    83221 ttctacagaa gatatttctg cgtaacctcg aaaatttttc aatgattcat gcaacatatc
    83281 tagatctgtc cctaatactg agacctttcg tacagtttcc aaaactagag aagttgttac
    83341 catatcaaca gtacgtactg gaattccagt gtctctttga atttgcgaat taaacgacgc
    83401 aagggacccc atatctacta agagtatgac cccgcttcct tcatctactt cttgtacatt
    83461 gcgttcaatt cggtccaatg ctgttttcgg atccatatcc aaaggcatgt ctactgctcg
    83521 tacgttctct gcatctaaca actgttggac aacttggacc atactgcttg ctgtgctgtt
    83581 tccgtgagct gcaacgacta caccgattct gccgcttgct tgatttgccc gtaacgagac
    83641 gagtagaacg gtcaagtaat agtcttcact ttctggaatc tccacttgaa aatacgtttc
    83701 gatataacta cgcacttctt tcgcaacagc atattcatct ggaaaatcaa aagccatctc
    83761 acggatgtta tcattcgtat gccgttcttc tcctaagttg atttttttca aaaaagaact
    83821 gatatgcaag ctcatagcat aaataaagtt ttgctgaaac tcatatggta aagatctttt
    83881 gaccattaca taaatctgat tggtcacttc tatgattttc ggatctacaa attctgctaa
    83941 tttgctgtct gcattaaaag aaaaaccatg gtctttatag aaagacttca gatggatgtt
    84001 gatatctgta gaaatgaaat gattgattgc ttcttgatcc aaaccatccg acttcagtaa
    84061 agctgcttta tctccgatga tatcatatag attataaggt aactcatagg agtccgattg
    84121 gattttcatg atcgtatcat ttggtgctac agttatcttt ggctccaata ttttagaaag
    84181 ctctgccatt gcactgcggt tgcttgctaa ttgaatcaat ccgctacgaa tcccttctgt
    84241 caaatcatcg attgtgatcg agatttcggg tgattgcata tgatttagaa atcctcgtgc
    84301 acatactaac tggatatttg acttcaattg tcctatattt ccataggtca cactgccgat
    84361 caatgctttt gctacatctt ctgtcaaaga aatctttcgc tgcgtgcggt ttgcttcgtg
    84421 ggcaatcatg atttttacta aatcgacttt ctcatttgca ggacgttttt caaaagccgg
    84481 aagtttgata ttgattggga tacgccgaac aaaagtttct aatagagaag aacctgggtc
    84541 ttctgtggtc gcacctacga tccgtacatt tgcttcatgg gatttcgttg tttccccaag
    84601 acgactgtaa gttccatgat ccataaagta aaagatcatt tcttgacctt caggcggcaa
    84661 ccggtgaatc tcatccaaaa acagcatacc gccatccgct tgatcgataa tccccatttt
    84721 ttcttcatct gcgcctgtaa aagcaccttt tgcgtaccca aataaatgac tcatcaaaag
    84781 ctcagggtta ttggcataat cagcacaatt gaaaacaatc agttccgttt cctcatctat
    84841 tacatgatgc gcttttgcaa aatgatacat cgcgtgtgca aaataagtct ttcctgatcc
    84901 ggtaggtcct gtaatcaaac aatttaatcc tcttggcgga tacaaaatcg cagcttttgc
    84961 ctgttccact gaatttttca tgctgccgtt agctccaatg atttttgcaa agatatcctt
    85021 agtatctatt tggagtgtat ttttttcttc agagaccggc gtttctttgt aactgactac
    85081 atgttttgta actggcttta cgtgcgtaac gagtgtttga tatatgtaac gtactggtcg
    85141 tccgtctgtc tttatcagtt ttccttcgcg cactagctga ttcagatctt tactgctatt
    85201 tgttcgctga atccctaaca cttcagcaat ttcctgtgtt gttactcctt gatcatattc
    85261 tgcctttgtt aaatgagcgg ttttttcttt gacatacata taaattcggt caatgcgttt
    85321 tgccaatggt atcgctcctt ttctatagac tattgtactt aggcttcaaa acataatact
    85381 atgtatttat ttctgtttcg tcaactattt acctgatttt gttttgttga taacacgcgg
    85441 atagatagaa atgttcaatt taaaagaacg aaaaatcccg atacgatttt tataatggaa
    85501 acaagaaaaa gaacactgtt taccatataa ttatttcact gatagcaaac aatatagctt
    85561 ccatcaattg aaaacgatag ttatcaaaac atcctgattc caaattggca aaaaaatatt
    85621 aagtgtaaaa agacaaagta aacttcgtag cctccactac aacttttatt tttataaaca
    85681 aaaaaggctg tgacaaaagt cttgtcacaa cctctttaat cataagattc aattaagctt
    85741 cttgagctac tggataaaca gacacttgtt ttttgttacg gcctttacgt tcgaaacgta
    85801 ctacgccatc aacttttgca aataaagtgt cgtctccacc gatacccacg tttacacctg
    85861 gatagatttt tgtaccgcgt tgacggtaaa gaattgatcc acctgtaact gtttgtccat
    85921 cagcacgttt agcacctaga cgttttgatt ctgagtcacg gccgttagat gtagaaccgc
    85981 cacctttttt gtgggcaaat aattgtagat tcatagataa caacatggtc tgcacctcct
    86041 atttatcatt aattgtttta atttggatat attctgagtt ttcagcttct atcgattgta
    86101 atcctaataa aagattttct aatagaatct cagcaatatt gttttgttct tgtgtcattc
    86161 ctgaagtgac ttcggtataa agataaccgc cttcttcttc attcatttca acaatcggct
    86221 caaaaccggc taatgaggca atcccgttga cagtgctgat cgctaacgca gaaacacccg
    86281 cacaaacgat atcacttcca taaggaccag catcagcatg cccggtcaaa gtgaatgaaa
    86341 cgatctgacc tgcgtcattt cgcttaaatg ttcctttgat catatatagg ccttctttct
    86401 caaacgctat taagcgttga tcgcgtcgat aaccactttt gtatatggtt gacgatgacc
    86461 ttgtttacgg tgtgaatgtt ttttaggttt gtatttgaaa gtaacgactt tcttttgttt
    86521 gccgtgtttt tcaacagtac cttcaacagt tgcacctgca acagttggag cgcctacttt
    86581 tgtagattct ccgcctacta agataacttc gtcaaaaaca actttttctc ctgcttcaac
    86641 gtcaagtttt tcaacataga ttgcttgtcc cacttcaact tttacttgtt taccaccagt
    86701 tttaataatt gcgtacatgc tataaagcac ctccttctga tatacttaga ctcgccatcc
    86761 gaagtgacag catatgctgt gcttattact tgttctgagc ggttgtagct gtggagtcca
    86821 caattacaac agtactagct taccagaaca gcaaccgtta gtcaactaaa tttaaaatca
    86881 aaaagtaaaa caaaaattca agcaatactt ctgcttttct atcctcacag ttctaccaaa
    86941 taaaatagtc gcattgtgtt tcttttattc ttataataaa agaaaaagga ggagcataaa
    87001 tggaataccc aaaagaactg atacaagaag cttctttaag aatgaacggc cgtcctgtag
    87061 aaggatttat cgctgggcaa ggacctcttc atcctaaact gatgctcgtg ggagaagccc
    87121 cagggaaaac agagattgat acgcatgttc cttttagcgg tcaagctgga aaagaattga
    87181 tgcaggcact ttcttcaact ggcttaacac gagaagaggt gtatatcacc agtgctgtaa
    87241 ggagccgccc gtatcgcgtg actcatcgga tcaataaacg gacacaacag tcagaaactg
    87301 tttatccaaa tcggacacct actcgttccg aagtatttgc gcatgcacca atattggatt
    87361 acgaactgat gaaggtccag ccaaaattaa ttgcgacact tggaaacatt ggtcttcaac
    87421 gtttacttgg caaagaatat acggttacca aaaatcatgg tcaactctat acagggcctg
    87481 tcttacaatt aacagaacaa aaggatgcct acgaatggtc agaaaaaaac tatcgaattc
    87541 ttccactttt tcatccagca gctattttct acaatcgctc ccttgtacct gttatccatc
    87601 aagactggca agtactagct tcacttttac agcaatatta aaaagaacag acaagaccat
    87661 aaaatcttca gtttacagtc ttgtctgttc tttttcattg ttttaatggc taattgcagt
    87721 atatttcgca tgtaaagctg tcaacgcatg agcaactgtt tgatgttgat gcgtgaatgt
    87781 atgttcccac aagtgagaaa gttgtttttc tacttctggc tgcaaggaag tatagataat
    87841 cgtttgtcct ctttgctctg ctttttcttg aaattcatca aaaactgcac gtgccgtcaa
    87901 atcaatactt ggtacgcctc gcatggagaa aatgacacca tcgtgatgat ttagttcttc
    87961 caatttattt ttgagttttt ctgaagacat aaaaaacagt ggaccactaa tatacactac
    88021 tgtccagtta ttcagctttt ccgtttctgg cagccccatt ctttgccaat cgatcgtttc
    88081 taatgaaatc gtgatagcag cgcttttgac aacaaaaaat acacagccgc cgatcacccc
    88141 gatcacgata gcaatactta aatcaaagat aactgtacaa atcatcgtaa ggataaacaa
    88201 tagaactgct gaccaatatt tattagtaaa tagctccttg atcgtttccc attcattcat
    88261 acgccaagcc gtcacaatca aaacacctgc aagtgctggc attggaatct gtgacatcac
    88321 tggcgcaaaa acaagcatgg agagcagtaa gacaactgca tgtatcatac cagctaatct
    88381 agtttgtgca ccagatttga tggccacgct tgtacgggcg atggccgcag tagcaggaat
    88441 cccgccaaag aaaggaagca acagatttcc aattccttgc gccattaatt cttgattgct
    88501 atctaagggc ttatttgcca ttcggccagc cgaagcacca catagcaagc tttcaatcat
    88561 tcctaataaa gcaatgctaa tagctggaac tgccacttgt tgcaaggcag aaaagctaaa
    88621 atctcccata ttcaaacgat tactactgat caatgtttgt ggaatcttac ccacagttgc
    88681 tactggtaaa tggaacaata gcataactgc tgttgccaaa atgatagcca gcagcgaact
    88741 aggcattcgt ttcgcccatt tttttggata gataaacatc cctaaaatga ctagccctcc
    88801 catgatcaaa gaggctgtgt ttatcggaaa tcctatcgtt tgataactac ttagtttttc
    88861 aataacgttt gtaccttccg agtgaacacc aaataaatta tcgatctgac ctaatgcaat
    88921 aatgatagca atacctgaag taaaccccgt aattacgggt gctgggataa aagaagtcag
    88981 tgttcctaat cggaaaagtc ctgctgcaag taaaaataga cccgctaaaa aagtagcaag
    89041 caatacacct tgcattccct ttgttgccgc aatagacatc aagatagctg ccatcgcacc
    89101 tgtcggacct gatatttgat aaaaaccgcc tgataaaccg ccaataacga taccggcaat
    89161 gatagccgta atcaaacctg cggccgcatc agctccgctt gataccccaa aagccaaggc
    89221 taaaggcaat gctacagcag ctactgtgat cccagccaaa agatctttct gaaatcttga
    89281 aagatcataa cctgaaaatt ctttttttag tgaggaaaca taatttttga acacctatct
    89341 aactcctttt tcatgaaatt ttctaaaata ccattcatta gaatatcata tttcatgaga
    89401 taaagtaata tttttcacaa agtttcctgt tagataaatt cttatttttt ctatatttat
    89461 aagaaaatag aaagcaatgc caacagagct agcccacctt gtttgagtaa aatccaacga
    89521 tcgctagtca aactgccata taatgcaaca aaaaaaatag atattaatag catacttgtg
    89581 atttctttag atgcggaaga aagataagct ccataaagta gtcccactcc taatagtcca
    89641 ttgtacactc cttggttctt aaacaatgtt tgaacagatt ttctgcttaa ctcttcttta
    89701 ctcattttaa aaaccctact tgtcgtgtca gaatcagttc tcaccgtttc gatatacatg
    89761 atataaaaaa attcaattgc aactagagtc actaaaattc tagatagtat cgtcatatct
    89821 tcttcctctt ttctgatgag ttttattttt ttgctcctta taattgtact cgataaggat
    89881 gtttttttat tttatttgcc tttgaaaaca aacaatattg gcacaaaaaa caaagaaaaa
    89941 ccgttagaaa cctcagtttc taacggtttt ttagcgtcac aggagggatt cgaacccccg
    90001 accgtccgct tagaaggcgg atgctctatc cagctgagct actatgacaa gaatcagcgg
    90061 aacatcaata attatagata attaaattgc ttctgtcaat acgaaatagt tatttaatta
    90121 gcaaataatg aattattttg gcccaactac taaatcaatc ttttaaaacg agacattctc
    90181 cttcagctgt tctaaggtta tcaattgaaa acaggagcta cattgacttc atgctactcc
    90241 tgtttattta gatttcctgt atcctttaat tgacttacaa atatagaaac ttttacatca
    90301 agttcgaatt gagccctcga ttcaatcgat tatctacagg ctgccataat catgtatcac
    90361 aactcgattg tttctgattc ctttgtcatt aagatcacac catcacctaa tggcaagatt
    90421 gtcgatgtca aatcgggatg atccatcaca acacgaagaa attcatttaa tttacgatgg
    90481 atagctcgtt tgccacgggg aatctcttca tctgggagca agatcgttcc tccttggaag
    90541 atgtcatcta ccatcaaaac gccacctttt cttaacaaac gtaagcattc aggtaaaaat
    90601 tcaatatatt ttgattttgc actatccata aaaataaagt catacggacc agacaactcc
    90661 ggcaagatat ctgctgcttg accttcaagc aaagtcactt tatctgtcag tcctagacgt
    90721 tcatagtttt ttttagcttt ctcgatcatg acatcgaaac gatcaattgt cgtaacatga
    90781 gcgttttctc cgattgtcgt ggccatgaca cttgatgaaa aaccgatagc tgctccgatc
    90841 tccaagattc gttcaggctg aagctgtcca agtaaaaatt tcaaaaatac aactgtttca
    90901 tggggaataa ctggtacttc tgctacatga gcttcttctt ccagtttgcc tagctcaccc
    90961 ggtaattttt tttgctgggt acgcataaaa tccaatagtt cttctttgac tacaggacga
    91021 tgcatcattt cattgcgcat actactcctc ctcttcacac attatacgaa caactgacta
    91081 aatctacaag ctgcatgagt gttttctcgt gtttcatgtt cagaagagtc gttggatctc
    91141 tggaatacat ttatgttttt aacattctta caaaaatttc tgtgttcgtc tccgcttctt
    91201 tttcaactct acaaaggtat tccatgctgt gattcctgtt aggcaaaaag caatcgtaaa
    91261 gaataacccc tgaatccatg caaatgccgt ttttgaaagg actagtccaa tatatggaat
    91321 ctgaaagatg ggactaccga taatatttct cttttttact actccaggct caatataagt
    91381 ctttgtatca gcttttgtat aaaaatcttc gtggttgttt tcaacggcta ttacgcgacg
    91441 cgtcgagatt ttttttcctg aattttcata atatgtaata atatctccga ctaaaatctc
    91501 atttgtattt ttcggtcgta catatatcag atttccttgt gcataatatg gactcatttc
    91561 tttgtcctca ataatataag gagtcaaacc aaaaattcgg ggaaccaaga cgacgagaag
    91621 aataagaatt aaaa
//
LOCUS       DACCWK010000002        63089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCWK010000002 DACCWK010000000
VERSION     DACCWK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.002"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            119..751
                     /locus_tag="IVW65_000115"
     CDS             119..751
                     /locus_tag="IVW65_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899049.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(885..1010)
                     /locus_tag="IVW65_000116"
     CDS             complement(885..1010)
                     /locus_tag="IVW65_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7899050.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1144..2037)
                     /locus_tag="IVW65_000117"
     CDS             complement(1144..2037)
                     /locus_tag="IVW65_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7899051.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFYSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2219..3145)
                     /locus_tag="IVW65_000118"
     CDS             complement(2219..3145)
                     /locus_tag="IVW65_000118"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7899052.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3230..3991)
                     /gene="pflA"
                     /locus_tag="IVW65_000119"
     CDS             complement(3230..3991)
                     /gene="pflA"
                     /locus_tag="IVW65_000119"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7899053.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4235..6466)
                     /gene="pflB"
                     /locus_tag="IVW65_000120"
     CDS             complement(4235..6466)
                     /gene="pflB"
                     /locus_tag="IVW65_000120"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7899054.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6743..9193)
                     /gene="parC"
                     /locus_tag="IVW65_000121"
     CDS             complement(6743..9193)
                     /gene="parC"
                     /locus_tag="IVW65_000121"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7899055.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9205..11250)
                     /gene="parE"
                     /locus_tag="IVW65_000122"
     CDS             complement(9205..11250)
                     /gene="parE"
                     /locus_tag="IVW65_000122"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7899056.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11416..11850)
                     /locus_tag="IVW65_000123"
     CDS             complement(11416..11850)
                     /locus_tag="IVW65_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7899057.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11977..12615
                     /gene="plsY"
                     /locus_tag="IVW65_000124"
     CDS             11977..12615
                     /gene="plsY"
                     /locus_tag="IVW65_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7899058.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12747..13622)
                     /locus_tag="IVW65_000125"
     CDS             complement(12747..13622)
                     /locus_tag="IVW65_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7899059.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13704..14495)
                     /gene="codY"
                     /locus_tag="IVW65_000126"
     CDS             complement(13704..14495)
                     /gene="codY"
                     /locus_tag="IVW65_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7899060.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14513..15913)
                     /gene="hslU"
                     /locus_tag="IVW65_000127"
     CDS             complement(14513..15913)
                     /gene="hslU"
                     /locus_tag="IVW65_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7899061.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15927..16475)
                     /gene="hslV"
                     /locus_tag="IVW65_000128"
     CDS             complement(15927..16475)
                     /gene="hslV"
                     /locus_tag="IVW65_000128"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7899062.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16512..17417)
                     /gene="xerC"
                     /locus_tag="IVW65_000129"
     CDS             complement(16512..17417)
                     /gene="xerC"
                     /locus_tag="IVW65_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7899063.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17615..18991)
                     /gene="trmFO"
                     /locus_tag="IVW65_000130"
     CDS             complement(17615..18991)
                     /gene="trmFO"
                     /locus_tag="IVW65_000130"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7899064.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18960..21038)
                     /gene="topA"
                     /locus_tag="IVW65_000131"
     CDS             complement(18960..21038)
                     /gene="topA"
                     /locus_tag="IVW65_000131"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7899065.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKKEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21160..22017)
                     /gene="dprA"
                     /locus_tag="IVW65_000132"
     CDS             complement(21160..22017)
                     /gene="dprA"
                     /locus_tag="IVW65_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7899066.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(22073..22840)
                     /locus_tag="IVW65_000133"
     CDS             complement(22073..22840)
                     /locus_tag="IVW65_000133"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7899067.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22833..23693)
                     /gene="ylqF"
                     /locus_tag="IVW65_000134"
     CDS             complement(22833..23693)
                     /gene="ylqF"
                     /locus_tag="IVW65_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7899068.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23953..24177
                     /locus_tag="IVW65_000135"
     CDS             23953..24177
                     /locus_tag="IVW65_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899069.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKQVVL"
     gene            24174..24350
                     /locus_tag="IVW65_000136"
     CDS             24174..24350
                     /locus_tag="IVW65_000136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899070.1"
                     /translation="MISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEMFSLEVQ
                     DLDGNTLEPVWMKM"
     gene            24587..25129
                     /locus_tag="IVW65_000137"
     CDS             24587..25129
                     /locus_tag="IVW65_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7899071.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25212..25763)
                     /gene="lepB"
                     /locus_tag="IVW65_000138"
     CDS             complement(25212..25763)
                     /gene="lepB"
                     /locus_tag="IVW65_000138"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7899072.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25915..26142)
                     /locus_tag="IVW65_000139"
     CDS             complement(25915..26142)
                     /locus_tag="IVW65_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7899073.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26139..26657)
                     /gene="msrA"
                     /locus_tag="IVW65_000140"
     CDS             complement(26139..26657)
                     /gene="msrA"
                     /locus_tag="IVW65_000140"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7899074.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26677..27390)
                     /locus_tag="IVW65_000141"
     CDS             complement(26677..27390)
                     /locus_tag="IVW65_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7899075.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27393..28289)
                     /locus_tag="IVW65_000142"
     CDS             complement(27393..28289)
                     /locus_tag="IVW65_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7899076.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28447..29289)
                     /locus_tag="IVW65_000143"
     CDS             complement(28447..29289)
                     /locus_tag="IVW65_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7899077.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29421..30074
                     /locus_tag="IVW65_000144"
     CDS             29421..30074
                     /locus_tag="IVW65_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7899078.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30137..30664)
                     /locus_tag="IVW65_000145"
     CDS             complement(30137..30664)
                     /locus_tag="IVW65_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7899079.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30684..31631)
                     /locus_tag="IVW65_000146"
     CDS             complement(30684..31631)
                     /locus_tag="IVW65_000146"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7899080.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31643..33529)
                     /locus_tag="IVW65_000147"
     CDS             complement(31643..33529)
                     /locus_tag="IVW65_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7899081.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(33689..35767)
                     /locus_tag="IVW65_000148"
     CDS             complement(33689..35767)
                     /locus_tag="IVW65_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7899082.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35780..36055)
                     /locus_tag="IVW65_000149"
     CDS             complement(35780..36055)
                     /locus_tag="IVW65_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899083.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36417..36872)
                     /locus_tag="IVW65_000150"
     CDS             complement(36417..36872)
                     /locus_tag="IVW65_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899084.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(37026..38234)
                     /locus_tag="IVW65_000151"
     CDS             complement(37026..38234)
                     /locus_tag="IVW65_000151"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7899085.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38346..38678)
                     /locus_tag="IVW65_000152"
     CDS             complement(38346..38678)
                     /locus_tag="IVW65_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7899086.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38826..39701
                     /locus_tag="IVW65_000153"
     CDS             38826..39701
                     /locus_tag="IVW65_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7899087.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39783..40364)
                     /locus_tag="IVW65_000154"
     CDS             complement(39783..40364)
                     /locus_tag="IVW65_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7899088.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40370..41629)
                     /locus_tag="IVW65_000155"
     CDS             complement(40370..41629)
                     /locus_tag="IVW65_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7899089.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41631..42677)
                     /locus_tag="IVW65_000156"
     CDS             complement(41631..42677)
                     /locus_tag="IVW65_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899090.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42912..43187)
                     /locus_tag="IVW65_000157"
     CDS             complement(42912..43187)
                     /locus_tag="IVW65_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7899091.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43430..44740)
                     /locus_tag="IVW65_000158"
     CDS             complement(43430..44740)
                     /locus_tag="IVW65_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7899092.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44922..46151)
                     /gene="rpsA"
                     /locus_tag="IVW65_000159"
     CDS             complement(44922..46151)
                     /gene="rpsA"
                     /locus_tag="IVW65_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7899093.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46286..46966)
                     /locus_tag="IVW65_000160"
     CDS             complement(46286..46966)
                     /locus_tag="IVW65_000160"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7899094.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(47015..47650)
                     /locus_tag="IVW65_000161"
     CDS             complement(47015..47650)
                     /locus_tag="IVW65_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7899095.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47716..49119)
                     /locus_tag="IVW65_000162"
     CDS             complement(47716..49119)
                     /locus_tag="IVW65_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7899096.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(49106..50137)
                     /locus_tag="IVW65_000163"
     CDS             complement(49106..50137)
                     /locus_tag="IVW65_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899097.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50168..50389
                     /locus_tag="IVW65_000164"
     CDS             50168..50389
                     /locus_tag="IVW65_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7899098.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50554..51291)
                     /locus_tag="IVW65_000165"
     CDS             complement(50554..51291)
                     /locus_tag="IVW65_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7899099.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51444..52784)
                     /locus_tag="IVW65_000166"
     CDS             complement(51444..52784)
                     /locus_tag="IVW65_000166"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7899100.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(53021..54544)
                     /locus_tag="IVW65_000167"
     CDS             complement(53021..54544)
                     /locus_tag="IVW65_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7899101.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54922..55533)
                     /locus_tag="IVW65_000168"
     CDS             complement(54922..55533)
                     /locus_tag="IVW65_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7899102.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55656..55781)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55863..56579)
                     /locus_tag="IVW65_000169"
     CDS             complement(55863..56579)
                     /locus_tag="IVW65_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7899103.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56585..57154)
                     /gene="scpB"
                     /locus_tag="IVW65_000170"
     CDS             complement(56585..57154)
                     /gene="scpB"
                     /locus_tag="IVW65_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7899104.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57138..57935)
                     /locus_tag="IVW65_000171"
     CDS             complement(57138..57935)
                     /locus_tag="IVW65_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7899105.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57905..58276)
                     /locus_tag="IVW65_000172"
     CDS             complement(57905..58276)
                     /locus_tag="IVW65_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7899106.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58295..59182)
                     /gene="xerD"
                     /locus_tag="IVW65_000173"
     CDS             complement(58295..59182)
                     /gene="xerD"
                     /locus_tag="IVW65_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7899107.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59278..59751)
                     /locus_tag="IVW65_000174"
     CDS             complement(59278..59751)
                     /locus_tag="IVW65_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7899108.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59856..60719)
                     /locus_tag="IVW65_000175"
     CDS             complement(59856..60719)
                     /locus_tag="IVW65_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7899109.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60915..63014)
                     /locus_tag="IVW65_000176"
     CDS             complement(60915..63014)
                     /locus_tag="IVW65_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7899110.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        17043 a        13308 c        10744 g        21994 t
ORIGIN      
        1 aaattaaatt ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca
       61 tatactcatc aatttgttat tatactttat atacttatca atttaatttg gagatattat
      121 ggaaattcaa cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac
      181 gatcttattt tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt
      241 aggctggttg cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt
      301 gattttgtct tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca
      361 tcgacatcct ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc
      421 ttttttagca attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa
      481 tggtttagtc gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca
      541 attgaaatgt ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt
      601 ttcctttttt gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg
      661 aaatctgttg gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa
      721 caaacaaaat aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag
      781 ttttaaacac ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc
      841 attcgtttct tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct
      901 tcccataaaa tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt
      961 cttatcacta cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt
     1021 ccagaccatg tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt
     1081 aaccataaag gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct
     1141 ctcttaggtt ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg
     1201 tataaatttc ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat
     1261 tgtcaatgaa ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg
     1321 taattctaat gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg
     1381 gaaaatacta ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc
     1441 tatttgatca aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa
     1501 tgccttcgtt acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg
     1561 gaagtgttct atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct
     1621 cagcaattct gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc
     1681 ttcgaaacaa tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag
     1741 tctcttcaat tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt
     1801 aggatcaaat attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc
     1861 atcgatcaat aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc
     1921 tcttagaatc gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aataaaaaag
     1981 cggatcatcg gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata
     2041 tgttctcttt tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga
     2101 acaagagtca taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg
     2161 tttcttttta tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt
     2221 agctaaatac agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa
     2281 gcaaagcacg gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt
     2341 taccagcaac aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca
     2401 gatcgtagcc atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat
     2461 ccatcacttc attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca
     2521 ttgggaaact ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg
     2581 ttcctgcttt caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa
     2641 gttcttttgc agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat
     2701 ctgaaatgat agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt
     2761 ctttatatag ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca
     2821 atggattagc tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta
     2881 cttcgctgat atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg
     2941 cattttctgc tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg
     3001 cgtattgtgt ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt
     3061 cattttgcaa gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt
     3121 gaccaaaaac taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata
     3181 atagcaaaaa aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta
     3241 aatattttgt atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg
     3301 taggtggttc gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc
     3361 ccatcgtatg ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt
     3421 tgtctagtcg gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa
     3481 tccaaaccgg tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt
     3541 cccaatgcgt tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta
     3601 aatctgtata cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag
     3661 gttttccgca tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt
     3721 cgatcaaaaa atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat
     3781 ctccccagta aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt
     3841 tccctccgcc aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc
     3901 cttgcataaa cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag
     3961 aatgaacata gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat
     4021 tttaagtaca agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg
     4081 cttcgttatg tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt
     4141 tctttcaaat aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc
     4201 gacgctaagt cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca
     4261 tcaagacatc taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata
     4321 cccggatcgt caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat
     4381 tactgaatac attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat
     4441 tacgcaagtt ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt
     4501 gggtatttga aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc
     4561 tagctaataa cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt
     4621 ttccagcttc acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag
     4681 taatcgtcaa taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt
     4741 gtcccatgaa gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc
     4801 cgtatttagg gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga
     4861 ttgcttttac ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag
     4921 caatacctgt agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt
     4981 cataagaata tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg
     5041 ctaaccaatc catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg
     5101 atgtgattgg acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat
     5161 tgatggcata taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt
     5221 ttccgacaac tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt
     5281 cacgaagctg attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag
     5341 catatgttct gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg
     5401 gtgaaggtcc catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg
     5461 aacgaccatc taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga
     5521 ataattggtt atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt
     5581 ggtcgatcaa ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga
     5641 tatagatatc aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt
     5701 tgattgctgc taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg
     5761 gtttagaaat atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat
     5821 attgttcagt gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag
     5881 tcatatcatg atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac
     5941 gacgatagtc accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt
     6001 tgtttttacg agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg
     6061 ttttgcgcca gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt
     6121 ttagcgcatt gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt
     6181 tgtctgtctg tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg
     6241 aagtaatagt tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga
     6301 tatcatttaa cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca
     6361 aaaagctgtc atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta
     6421 cgccttcttt ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct
     6481 aaatttctgt tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg
     6541 caagggtttt cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc
     6601 aacgttgaca taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat
     6661 aaggatttat taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct
     6721 cttcattaaa atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt
     6781 cgatgacttc tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac
     6841 ctatagggaa atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta
     6901 tttcttttga tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac
     6961 gcatgaccgt cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc
     7021 gtttaacagc tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa
     7081 gcagcccgtt gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct
     7141 ttgctccaac aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta
     7201 ctaagaaaac atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt
     7261 cttccgcctt tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga
     7321 actctgtcat tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct
     7381 ctgcttgaat tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg
     7441 agattgtttg agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga
     7501 cagggcggta gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca
     7561 attcacctga gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag
     7621 cactataaga acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta
     7681 cgtcttcttg tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga
     7741 ttgtcgttag acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa
     7801 cagagaaaag ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca
     7861 agctttcgga ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca
     7921 atgtgacaat tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt
     7981 tggcgtcttt tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata
     8041 aagctttcat caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt
     8101 gactacgttt tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca
     8161 gtccaacttg atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct
     8221 gtagctcagt gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca
     8281 attcgacgac aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat
     8341 cgattttctt attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt
     8401 catatgggat ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg
     8461 ttttcgaacg aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt
     8521 ttccttgcaa gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt
     8581 caaggctcgc ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt
     8641 tatgagtcgg gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa
     8701 ggtttgggta tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca
     8761 cgagatcgac tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc
     8821 tggcttcagt ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt
     8881 gcatttcgat cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat
     8941 aaatactgcg gtcaccatga gggtgataat tccccatgat atttccgaca gattttgctg
     9001 atttacggaa tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc
     9061 gttgcactgg cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt
     9121 tggaataacg tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt
     9181 gtcgattttc catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt
     9241 atctgctcgt tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc
     9301 ttttttatct aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca
     9361 ttttcttcga ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc
     9421 agcatcatcg atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca
     9481 caattgttcc gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc
     9541 gactttttta atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt
     9601 ctttccttgt cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc
     9661 ttcaatcagc ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc
     9721 gccgtctgta tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga
     9781 aaactcagga ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt
     9841 caagatatct tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat
     9901 tgcttggaac tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac
     9961 taaatacaat tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag
    10021 aagagattcg ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc
    10081 tgcttctctc gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct
    10141 attttcctgc aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc
    10201 aactggcgta ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac
    10261 acggatagaa agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc
    10321 ccgttctttc agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc
    10381 tttcatacca gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa
    10441 aacattttct gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt
    10501 ttctcctgaa aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac
    10561 aaattctttg attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag
    10621 atcagaaaga gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc
    10681 taaaatttca tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac
    10741 agtcgtcccg tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc
    10801 gccgttttta aatttctgct gatactctac gccatctcta acgatcgtta caattagcca
    10861 tttcgataag gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta
    10921 ccctccttga ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg
    10981 tattccggaa gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact
    11041 attgtcttca tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac
    11101 tgcattatcg acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat
    11161 gtacatccct ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc
    11221 attgtattcg ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg
    11281 caaaaccgca ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag
    11341 aaatcaattt taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct
    11401 tctttgtggt catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca
    11461 acgatccatt acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt
    11521 ttcaatccct aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat
    11581 ttcaggtaaa aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc
    11641 tgtcaaattc gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac
    11701 tggtatgatt ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc
    11761 tggtttattg ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat
    11821 cgtttcttgt gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc
    11881 catctattat acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa
    11941 ataaaggata gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt
    12001 ttagcatatc ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa
    12061 aaagatatcc gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta
    12121 ggcaaaacag ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact
    12181 tgtttgccga tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc
    12241 attcttggcc atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca
    12301 agtgcaggaa tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt
    12361 atcgttagtt tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg
    12421 atcactttat ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat
    12481 tggttactga cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac
    12541 aatatcaagc gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc
    12601 aagaaaaaat cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga
    12661 tgtcacactc ccttattact tacaggatag cttttttaca caccttggta aacggtgttc
    12721 cctgctaact tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag
    12781 atttcatggg cacctagttg attgatccct actttttcca tcaagtttcc actactgttc
    12841 accgtgtcag cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa
    12901 ggagaccaga tccctacata aggcatatct ttatagctta aagttacgct atgggaagat
    12961 ttttcacttc gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc
    13021 tcaaaaagtt cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc
    13081 atatcgatga atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc
    13141 agatagaaat cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca
    13201 ccgatagaaa agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg
    13261 ccgtctcctc ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat
    13321 gtctccggcg tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca
    13381 aaatccatat cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca
    13441 tactgatcat cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa
    13501 tacgctggat caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc
    13561 aattccgcac cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc
    13621 attgccattc ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc
    13681 tatctgcccc agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg
    13741 gaattcaata cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca
    13801 gctgattcca attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg
    13861 gcaatattag aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt
    13921 acagccttca attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc
    13981 cgtacatctg cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta
    14041 gctccatatt ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag
    14101 ataatcgtac ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat
    14161 ggatatttct ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta
    14221 gcttcagttt tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca
    14281 acaaacattg ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat
    14341 actccgtcac ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc
    14401 attttatcgt aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg
    14461 tttatcttgc gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa
    14521 tatagcgact taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt
    14581 ctgtgatcgt tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta
    14641 gtttttccaa aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat
    14701 cgaaagcgat ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt
    14761 ctgtaccgat caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct
    14821 tcacaaaatc ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa
    14881 gttctggaat tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga
    14941 cgtgatctgt ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca
    15001 atatgtctct ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga
    15061 tcttatcgat ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac
    15121 tatggatatc agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag
    15181 cttctttgac tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt
    15241 catttaggtc gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt
    15301 ggtcttcaac ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca
    15361 agactgtacg gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat
    15421 tttgctgact agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct
    15481 gttctttttt gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag
    15541 cacttgtgta tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta
    15601 aatcccgaac cattgattct acatctcttc cgacataacc gacttcagta aattttgttg
    15661 cttcgacttt gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt
    15721 taccgacccc tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt
    15781 tttcatctaa ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg
    15841 ctgcgtgctg tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt
    15901 ttaattcata catatctact cacctcttac agttcttcta cgataatatt atgattagta
    15961 aagatacaga tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg
    16021 tcgttttgag catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat
    16081 ccgatagcta agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc
    16141 atttctttat cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc
    16201 cgccattctt gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt
    16261 ttgccttcaa atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca
    16321 acaacttctc cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat
    16381 tcacccattg ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca
    16441 cagatagtgg ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct
    16501 tttccttatt tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt
    16561 gttacatggg catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact
    16621 gtccgcaaat ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga
    16681 ggatggatat cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt
    16741 ccagtaggcg tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga
    16801 tattttgtca tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca
    16861 aaagaaccta gaggagcata tctctctttg ttccctttac catggatcaa catgacctct
    16921 gaatcaaaat caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac
    16981 ccgtaaagga cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg
    17041 ctgtcaaaaa gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc
    17101 tttttgagat gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac
    17161 tgataaaacg aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga
    17221 cgatgatcag ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga
    17281 tcagcgtctc cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct
    17341 tctttcgttt tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc
    17401 cacgtggctt ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg
    17461 tttataaaaa gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat
    17521 tggcgcaatt cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg
    17581 agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt
    17641 ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc
    17701 ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca
    17761 tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg
    17821 tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag
    17881 caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa
    17941 agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca
    18001 taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa
    18061 tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt
    18121 tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc
    18181 cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc
    18241 ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag
    18301 atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt
    18361 ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata
    18421 ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa
    18481 aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc
    18541 ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta
    18601 ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag
    18661 ctaaggcacc accagctggc actgctgttt catctgccga agtgattact accgaattca
    18721 atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg
    18781 aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg
    18841 gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac
    18901 ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat
    18961 tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg
    19021 actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc
    19081 caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta
    19141 gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt
    19201 acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat
    19261 ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca
    19321 tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt
    19381 ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa
    19441 tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt
    19501 aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca
    19561 tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg
    19621 ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa
    19681 tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga
    19741 atattttctt tctctttttt gccgtcatct ctaccttcca catacacttg catgaatcct
    19801 ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact
    19861 ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat
    19921 gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc
    19981 acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt
    20041 cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct
    20101 gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact
    20161 gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat catggttttt
    20221 cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga
    20281 ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca
    20341 tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg
    20401 tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg
    20461 ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt
    20521 aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata
    20581 ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact
    20641 aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt
    20701 tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc
    20761 caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt
    20821 tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg
    20881 ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg
    20941 ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt
    21001 gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat
    21061 tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca
    21121 gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa
    21181 gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt
    21241 agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc
    21301 ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt
    21361 agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg
    21421 aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt
    21481 ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg
    21541 agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac
    21601 atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga
    21661 agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt
    21721 ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc
    21781 aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg
    21841 ccaacttttg cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc
    21901 aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc attttccttg
    21961 actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat
    22021 aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta
    22081 aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa
    22141 gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat
    22201 aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag
    22261 cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat
    22321 ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt
    22381 ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa
    22441 cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct
    22501 ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga
    22561 taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg
    22621 caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt
    22681 tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca
    22741 cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc
    22801 ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct
    22861 tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat
    22921 tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat
    22981 tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct
    23041 gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc
    23101 tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg
    23161 ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc
    23221 tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga
    23281 ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga
    23341 ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca
    23401 ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct
    23461 tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta
    23521 ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa
    23581 gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca
    23641 gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc
    23701 tccattcctt tcataccgga atattccccg gctttacttg tttttttctt tttcaatata
    23761 aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc
    23821 tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat
    23881 gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag
    23941 gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt
    24001 tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa
    24061 gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat
    24121 aaaaaattta taaaagataa agaaatagct gataataaaa aacaagtggt gctttgattt
    24181 cttttagtgt tgaaaatgcg gatgaagtaa aaaaatttgc tcaaattgca aaagaaaatg
    24241 gtggtacttt ccacgaagta gatatgggaa tgcctgaaga agaaatgttt tcattagaag
    24301 tccaagatct agacggaaat acattagaac ccgtatggat gaagatgtaa gcagataact
    24361 aatctgacgt tttttataac aaaaaaacaa aggcggatga tacgataagt attctccgcc
    24421 tctatttttt ttattcgtac aatctctttt ttacagttta taaaagcagt cttctcaccc
    24481 tttttttaat tgttagatgc cagtgtatgt aaatcaccaa tgtgtcatga cacattggtg
    24541 attttatggt atccttttat tattaactga caaaggagag aatattttga acactttgat
    24601 tgcaaaagac gtttttagat tgcccgaaat gaatgcaaga ttgttgtcga cctctgctct
    24661 tttattagcc cttgatgtca tcttatacaa gatagcgatt ggtcctgctt attttcaaat
    24721 cacattcgga tttttaagta tggcgcttat gggttatcta tatggaccga tctgggctgg
    24781 tttgcttgaa atgttgtcga acactttaaa tttcacgata tttggttcag gaacgtttca
    24841 agctgctttt cttatcacgg ctttccttgg tggatttatt aatggcttat ttttatatca
    24901 taaaaagata aacctgcctt ccgtcatcgc tgcccaatta ttgattatga tcccagtcag
    24961 cctttttatc aattcttggc tgatgtctgt tttgtttggt gcagatttca aagtaatttt
    25021 tctaggtaga ttcatcagga atatgatcca aatccctatt caaataatgg ttgtttattc
    25081 atttatcaaa actttagagg ctagaaaagt gtttaaacga gctgtttgat tttacgtact
    25141 aaggtagtaa actgattttg gattaaacaa taaagcgtgc agtctcaaca gactgcacgc
    25201 tttattgttt ctcactggat ccattttatt tcatcaagtg gataatagac aaatcttgct
    25261 ttacctagaa tatcagtttt attgatcgtg ccaaacgaac gactatcttt tgaaactctt
    25321 cgattatctc caagtacaaa ataattatct tccggaagct tggattgtcc cgttagctct
    25381 tgtagtgtaa agtccgtcgt ataggatgca tttgcatgat ctgatttcag gtttttagtt
    25441 agataaggtt ccttaactac tttcccattg atcttcaatt gatcattttg gtagctgaca
    25501 ttttctcctg gaagaccgat tactcgtttg atataaatcg tgccatctgc taattgaaaa
    25561 acaacaatat caaatcgtct gatctccgaa aatttttcca tcacaaccat atcaccttgc
    25621 ttcaacacgt tttccattga attgccttcc actggtaccg ggatgaataa aaatccccgc
    25681 aacatgaacg caaggaatac agaaacaagt aagtatttga atacaagcca gaagcgatta
    25741 atatagcgtt gtttttttgt caatctctat caatccatcc ctcttttttt cattctttct
    25801 ttattataag aaaaatatca aaaaaatgcg actagatagc tgaaataaaa ataattcttt
    25861 caattaggct attggacaaa aaactttttt gatcataaag tacaaaacta tctcttatat
    25921 gtcatgatta ttttctaaat atttttccca tgcttcgtca aagatatcca tggacggcaa
    25981 atagtcgaca ttcagttcca gataattaga aatttcttcg tagctagagc tgtgcttagg
    26041 aaattggatg tctttaccgg cttctgtagc gaaaacagat aaagcatcat gtgtgccacc
    26101 gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt cctccaattt
    26161 tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa aattttcagg atgcttttta
    26221 taatactctt gatggtcttc ttctgctaga taaaatggtt gggctggttc gatttttgta
    26281 acgattggct gttcgaaacg tccactttca gctaactgtt tcttactttt ttcagcaatg
    26341 atccgctgtt cttctgtcaa ataaaagatc accgggcgat aattgtcacc gcgatcctcg
    26401 aattgtccga aagcgtcagt tggatcagtt tgctgccagt aaagttctac taattgttca
    26461 tatgaaatca atgttggatc atagataatc tccactgctt ctgtatgtcc agtcgtttgg
    26521 ctttttactt gttcataagt cggatctggt acatgccctc cagtatatcc tgacatcact
    26581 gtatggatac caggaagcgt gtcgaaaggc tggatcatac accagaagca gcctcctgca
    26641 aatattgctc gttccattat gattccccct ctaatactac tcatccgatg attttttatt
    26701 ttcttttgtc ttgctttcag tactgctggt gctagttttg ttggtatttg agttgtttga
    26761 agcatcagag gtatcttttg gcaaatatag actaaaccga atgtcatcat ttactaagtc
    26821 gattttatct gctttaatat acatcccgtt ttgcattcgg aattgatcca acctaagcat
    26881 gatagtcaga tcatctgtat tgacttcgat ccaatctggt aatttatagt ttctcttgat
    26941 catattcatc acatctttga ttggcaaact caatgttccg atagaaagac ttctagcttt
    27001 tagttggaca tttccgtttt ccatcacata cggatcaaaa tataaataga aatcgatagg
    27061 aaaacctagt acttcgaaag taccattgag tagtgcttca ttttccaaat aaaatttata
    27121 ttcaatatca ctatcttttt gatactcact aaggaaaaag ctgatgattt gattgacttt
    27181 ttctttattt gaattgatcg ttaagacagg atctccttgt ctttccgtaa gagctggtat
    27241 ttctgggagt tctggttccc gattagcaaa aatacggctg cctaaaaata cggtcgaacc
    27301 aagtactacc ccaagaagga taaggaaggc gaatttccag taatttgttt tctttgtttt
    27361 gtttgaagat acgtcagatc ttgttttcat gtttattgat catcctcctt ctctaaccat
    27421 tggttttgtg tctcgatcag ctgatctctc actgcatttg ccatcaattg ataacctaaa
    27481 ttatttggat ggaaatgatc ttcttcatag agcacattat ttttgatgcc atttagattt
    27541 tctgtcgtgc tagttgtttg atctgttcct tcttctgtga cacctacttc ctgattcaat
    27601 ccttgataaa gcaaatcatt gattgggata aagtagcagt tcggcatttt ttttgtcacc
    27661 gtttctgttc ctttgttcca attgttaaca attgtctgca tgtcagtgat ctcaggaaag
    27721 ttcaaataaa atggattgta gatgcctaaa atgtaaatag gtgcggtttt attgagggat
    27781 cgaaattctt ccaatagttc tgtgacattt tcctgatagt ttttcagtgg ctttttaaag
    27841 gaattgaccg ttaacccgaa taaattattt tggataactt tcatcaaatc attaccgcca
    27901 acagtcagtg tgataaaatc agctgttttg atattgtttc gaatttcttc ttttgttttt
    27961 actcgcttta agatctggtc acttcgttca ccatttacac catagttttc tgtttcaacc
    28021 gcggtcaaat tataacggtc ttttagatca tttgcgacaa ggggaacgaa tcctccttgc
    28081 ttggtttgat ctcctactcc ttcagtcagc gaatctccga tagctatgta gtgaagtttt
    28141 tcttttttat ttggatcttc tctcacagta gttactggtt tcattagtgg gccagctttc
    28201 ggtatagaag cagctaaacc tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg
    28261 aggatcaaat gtcgtttcgt taatttcatt ttttcacctc ttgtattcga ttggttctaa
    28321 tgttatcaaa aattttatca aaaaggtagt ctttatgaaa aaagaaaaca attattttcc
    28381 taattttata aagaaaaaga gaataagctg caagcctttc aggctgaggg cttatcctct
    28441 tttcgactaa tctacataat acatgattgc aaacgcgtct ttaccagtat gcgtagcgat
    28501 aaccggattc gtatgcagta cagggatatg catgtccggg aataattctt gcaaaccctc
    28561 tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt
    28621 tggcaattct ttcagttcat ttttcaaatt attaaaccat ttatcgaacg ttttctttcc
    28681 tcgcccctta gttacaggga ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt
    28741 caaaacacta gacagcaaac cagttgttcg gctgattctt ccacctttta ccaagttatc
    28801 tagtgtggat attccgatgt ataattttgt attgtcttgt acttttttga cttcttcaag
    28861 gattttctct actgttttac cttcttttgc taattcagct gctcgaatta cctgaaaaga
    28921 taatccttgg tcagtggtat cactgtcgat aaccgttact ttcgtttttg ttagattact
    28981 ggcttgtctt gctgcttcta ctgttccact caagcctttt gtcatatgga tcgaaatcac
    29041 ttcactgccg tcttctccta gctcatcata catcgagatg aattctccaa ttggtggttg
    29101 gcttgttttg ggcagatttt ttgctttcgc catcatttcc ataaatcttt cgccgtctag
    29161 ttggtcatca tcgggatata ctgttccgtc gatcatgatg gatagcggca tgacacgaat
    29221 gcctaaagca tcgcgtacat ttttttccat cgtacatgaa gaatctgtta cgatttttac
    29281 atttgtcatt attttctctc tctctttctt taacaagatg ccttattcat gttaaactac
    29341 atgtaagaca atttctattt ttagtataac agacaaataa tgatttttca ttaattacac
    29401 ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa
    29461 atgaagtgtt taatgcagtc acccatggaa ttgggactgg attgagtatc gcgggactag
    29521 tggttctttt agtaaaaggt gcccacttgc attcctccct ccatattgtc tcttatgcta
    29581 tctatggatc gatgatgatt cttttatttt taacgtctac tttgtttcat tcattgattt
    29641 ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt cttttgattg
    29701 caggaagtta tactcctttt tgtttgctga gtatccgagg atggctaggt tggactttat
    29761 ttgtattaat ctggttgttt gcgattcttg gcattatata taaagcgttg acgttgcata
    29821 aacgggagag agtatcaaaa gtatctacga tcatctatat ttttatgggg tggctctgtg
    29881 tcgtggctgc tgttcctctt tatcactctc ttggaatgac tggtgttatt ttgatggtag
    29941 caggcgggct ttcttacacg ataggcgcat tcttttatag cttgaattct atccgctata
    30001 tgcatgttgt ttggcatttg tttgttatgt taggtgcagg ttttatgtac ttctctgttt
    30061 tattctttac ttaataaaaa aaataaagct atggatggga cagctctaaa taacagctag
    30121 tctatccata gcttttttat atcgtcaatt cgcctgtccc gctttttaca ggattctcat
    30181 tacgctgata cgtttcaaat tgatgcggat aacgattttt ttcatccatt tctccttgac
    30241 tagtattgat cagtgaccaa tcagaccagt caacaggtgg aaaataagca tctccatcga
    30301 atacatgatg gatgactgta cggtataata tcgtacaaaa tggcagaaat tcttgataaa
    30361 cagctgaccc tccagcgatg aaaaagatac cgtctgtttc tttttccaga gcaagcacgt
    30421 cttccacact atgcatgaca tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa
    30481 caatcgtctg acgattcggc aacggacggc tacccatccc ttcgaatgtt tttcgtccca
    30541 tcactagtgt atttgcttct gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc
    30601 aaggaagacg gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta
    30661 acattttctc atcctttctg attctaaaca gcgataggag ctttgatcgc cggatgtggg
    30721 tcatatcctt ctaattggat atcttccatc tcaaaatcaa atactgactg cttttcctga
    30781 ttcaaaacta aagtcggaaa tgcgcgtatt tctcggctca attgttcttt catttgttcg
    30841 atatgattga tataaagatg agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa
    30901 cctgtttcat gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg
    30961 cctagaaaca catccccact gcgttgatac aattggcagc ttagccgtcc ctcttgaaca
    31021 taaaattgaa acatcgtatg acaaggaggc aaagccatgc taggaacatc ttccggattc
    31081 catgcagaaa cgatcaatct tctagaatca ggagtctttt tgattgcttc gatcacttgt
    31141 ttgatttgat cgataaagcc accgtctttt gtttcccagt gacgccattg atagccgtag
    31201 atgtgtccaa gatcgccgta tgtttcagca aaggaatcat cggttaggat cttctcgcaa
    31261 aaaagcttgc tctgcttttt atattcttca ttgaattcag gatctgtcaa acatctatgg
    31321 ccaaaatcag tcatatctgg tccattatag tctggacttt tgatgtagcg ttcaaatgcc
    31381 cattcatccc aaatatggtt tttattttgt aatagatagc ggatattcgt atctcctttt
    31441 aaaaaccaaa gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa
    31501 ggaaaccctt tttgcagatc aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg
    31561 cctgttctgt cttctttacg atgcccttct tccaacaatt ttcttcctaa gtcaagatac
    31621 gcttgttcca tcctttttcc tcctaatctt ctacccattc actcaagtat tcccaccgtg
    31681 ccattttttc ttcaagttgt gcttctgttt cagatacgtc gttttgcaat tcttgtaatc
    31741 gagtgaaatt atctccttga tgattcatct cttcttggag caatgagatt ttttcttcaa
    31801 gttctgcaat ctcatcttca atcgtttccc attctttttg ttccatgtaa gataatttct
    31861 tcttttcagc aggttccttg ctctgtattt ttggtttcgc tttttcagca ggctgctggg
    31921 tctctttttg cttcagtaaa taactggaca tgtttccgta ataaagttct tgttttccgt
    31981 ttccttggaa tactaataac ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa
    32041 cagtgatcac agcacctgga aaacttctaa aataatcttc caaaatcgtc aatgttgcta
    32101 tgtccaaatc attggtcggt tcatccaata ataaaacatt aggttgctga atcagcagtt
    32161 tcaacaaaaa taaccggcgt ttttctccgc ctgaaagttt gcctatcagt gtcccatgca
    32221 taaatcttgg aaataaaaat cgttcaagta attctgctac tccgatttga ctgccatctg
    32281 ctgtttttgc ttcttcagca gcttcttgta agtaagcaat catccgttta tctggtgcca
    32341 tttcttcatt ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata gacattgtac
    32401 cgctatccag gggaattctg ccagctagaa tattaagcaa tgtcgatttt cccgcaccat
    32461 ttttaccagt aatacctagt ctttcccgag attgaatcaa taaatctaaa tgttctagca
    32521 gtgtttggtc atttatacgg taactgccat ctttgatttc cagtactttt ttccctaaac
    32581 gttgtgtcgc taggttcagc tctaaatcat cttcttggtt tacttggtac aagttctcct
    32641 ttagatcttc gaaacggttg atccgtgctt gctgcttggt gccacgtgcc tgtacgccag
    32701 ccctcatcca agctaattcc tgtttataaa gccgcttgcg cttttcttct tgttctactt
    32761 ctacacgatc acgttctgct ttttctaaga cgtaagcttc ataatttcct ttatactcgt
    32821 ataattttcc aaatgaaagt tcgaagattc ggtttgctac tctatctaga aaataacgat
    32881 cgtgggtaac catcaatagt gcgccacggt attgtttcaa atagttttcc aaccactcga
    32941 tcgcttcata atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa
    33001 tcaacacttg cgccagacta actcgttttt tctgaccacc tgataaagta ctgatttttt
    33061 tatctaattc actgattcct aatttttgca aaataatctt tgcgttggta tctgtagtcc
    33121 aagcatcttc tttattcatt tgttcttctg ctttagcata acgtttttgc acatcagggt
    33181 cttctccgtt ttcccctagt tcgatcaatg cttcctcata tgctcgaaca gtttggatca
    33241 aggggctgtt tccttgaaag actgcttgga gaacagttaa ttcatcggaa aattcagctg
    33301 cttgagaaag ataaccaata cggtaatcag ttggataaaa cactgtttgc cgatcaccgt
    33361 cgctgctatc gataccggca ataatattta agagacttgt tttacccgtc ccgttcgtcc
    33421 caatcaaccc gatacggtct ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt
    33481 tttcaccata agtttttttg aggtcagtta cttttaattc tttcaacatg ttccctcctt
    33541 atcttggata ttgtatcata aaaatagtga acttcaggaa aatttttttg gtatttgttc
    33601 tttttgtgtt ctcaaaaaaa gaacaggacc gattcgatcc tgttctgata tgttttgttt
    33661 tttataaaac ttgaaaattt ttaccttttt attttattgg actcgttttt tttgactttg
    33721 ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt gttcatcgta taagattcta
    33781 acaaattgat attcctgcga tagctgttcg cagaaaacgt cgtgattttt tcagcaactt
    33841 cgtattcatc aatgattttt ctttcaagat aataacctct tagcattttt ttcgtaaaca
    33901 gactatcttg ctggatcatc cacgaagaaa tcaaattccc cgagttcatt tttattcctt
    33961 caagtttgcg ttgctggata aaaaattgaa ccatcgtttc atcataaata tcagtcaagt
    34021 tatctaaact ttcaataatc aactgagtat ttttttgata tgtctctctg atttcttgga
    34081 tatcttcatc actaataaat gtctgacgat cagcatatct tctttgccaa aacattttcg
    34141 gatggagaac gattcgcatt aacctacgga caacaatgaa ccaaaagccg ataaaagaca
    34201 aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa acgggagtaa aaacgataac
    34261 cattagaaga aagcttgccc gaacgataac tttcatccaa tccatcccgt tcgatcgaca
    34321 aaatcagtgc ttgtatttct tgtacttctt gcttttcatc atcggttaaa tcttccaagt
    34381 acagatcacg tatccgttct tgataatttt ctatcactgc ttcagtggct aaaaattctt
    34441 ttgtattacg atcaatttcc tttatttctt ttcgcaaaac ttcaacgacc tcttctaaaa
    34501 tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt ttccgtcagc ataggcaaga
    34561 caatgatccc tatgaccaaa gtcactaaga tgacacccgc cgtcagaaac aaaagcaaag
    34621 accgctggta aaacatcata ttattgattg aaaatggcaa aataaaaatc gtagccaaac
    34681 tgactgttcc ctttacaccg ccaaatgtta agatcaagat ctcgttcaac tgttttttga
    34741 actttttact gccatctttg aatacataaa aaagactgat agaaatgaat ctgatcacaa
    34801 aaagcatgac agagattaat acgataatcg ctaataaaag accgtttgga tataaaccgt
    34861 ctccccatac tggcgagaac acttgcgtca attcgattcc taaaaatata aatactaatg
    34921 cattcagtgt aaaagcaata gttgtccatg tactgtggga caagctcgac aattctgcat
    34981 caaacacaga gattttccta aaaccagaag cttggagaat acctgctgcc actgcagcaa
    35041 tgattccaga tacatcaaat gcctctgcca atacataggc tagaaaagga agcaaaagtt
    35101 cgatcagcag ataccctgtg acatcttgtg ctgatgcttt ttcgatcaaa tgaattattt
    35161 tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc cccaatacta gaaactacta
    35221 gactcatccc cgcgtttact gctgaaaaag atccggtgat cagcgcacca agagcaaact
    35281 ggaaggcagt aacaccagat gcatcattca aaagaccttc tccttccaaa atatgcatcg
    35341 cctttttagg gatattcact cgtccagaaa gagaactgac tgctaccgca tctgtcggac
    35401 ctaacgctgc tccaaaagca aagcaggcag caagtgggac acttggcaac aggaatgata
    35461 acgtcgcacc taccccgaca agggtcaaaa tgactcctcc aaatgccaag aacaaaattg
    35521 tcccgaaatt tttgagaatc gaagaaatat ctgctttttc tccttctcta aataacaatg
    35581 gtgcaataat cataactaag aacaattcag gttcaaaatc gattgattgt ccccactctg
    35641 tcagacctaa taccactcca aggaaaattt ggatcagtgg agctggaatc attggcaaaa
    35701 tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa tacaataact aactcagcaa
    35761 attccatcgt ccatcccctt tattttttat tttgtgtaag catttttaaa aattgttctt
    35821 ttttctttaa aattttggtt tcgactactt caacctgtgt ttggttcccc tctaattcac
    35881 actgtacacg ttttgactcc aaacgaagca gatcatgttt cagacgttcc aactgacgat
    35941 acatcaatat atttctttta cgcaacttac aaagtatttg acggcggtca tccatcgaaa
    36001 gggcgccata gcgatcatgc aacagttttt tttgttgttc aaaactctct ctcattttcc
    36061 agatctccta tacacaaact tccatcatta tagcaaagtt ctttttggat gaaaaatatt
    36121 tcattgtttc atcgtttatt gctagttaga aatttccata aatttttcaa cgcctcattc
    36181 tattccaaat agtatcagat atcaagtaaa acttaagaaa gataaaagtt tctgacaaaa
    36241 ggatgtttac tttcccattc ctgcctttct tgttctttta tcacaacaaa aaaatggggc
    36301 tgcgacaaaa atcttgtcac agtcccttat tctgaataaa cggtgtggaa gagacagcct
    36361 atcagtagta ataagacaca tctctttcac agcctcattt ttgcgtaaaa atcgatttat
    36421 tcgaaaaatt ccccgtatac atcttctaca cgttgatctg gattgatcac gatatacaag
    36481 ttacctgttt tttcgctgat ttttgttgat tgatattgat tgtctaatcc ttctgttcct
    36541 ttatctagat atgggaagta cacaagatca ggtgtgccat ttcggtaaag gatttccatt
    36601 cgctcgatat cttcgtattt caatgcgcga ttaaacatgc ccatttccag tccgcctagg
    36661 ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt aaatccagca
    36721 cacggatgga tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt
    36781 ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa
    36841 taaaatttta gcgctgtcat ttctttactc attaaaaaga ctcctttatg atgtaattta
    36901 ttcttgtact acctcagttt agcacaaatt tatgtaagtg gtcacatctt atgttttttc
    36961 ttctaatgac gtatctgccc actttaagta gccaacaaag ctgagcagat acagtctagt
    37021 gttttttaaa tcaatttttc tttcgataaa aaatccaaaa cctgtttttt atcatttttt
    37081 attctttgtt ctacgacggc tttttcagct aattgcaaag cttcactaac ccacttacca
    37141 ggtttttttc cagtttcttc tagcaagatt ttaccatcta ctgctaattc gcttaatgac
    37201 ttgataggta aacgatcata ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa
    37261 tagatcatca attcttctac ttgcagtgca tgttttttgc ctaagaagta caattcgaat
    37321 ggttccaaag ctccagtttc tcgttgtcgt agtccttgga caagtgcttg aacgtcttta
    37381 atcatttggt tagaacactt ccacgatttc aaaaaggaac gaatctcatt ttcttttaga
    37441 tccagttggc tgatcaataa tgcccatgct tggctctctt ctttcaatgg gacggatggt
    37501 aattcagcaa aacgtaaaag tgcttcgcct tcttctttca aaccgggaca gcaaatgtag
    37561 cactctgttt caataaatgc atggattccc gcggcccgtt catttcccaa gagcatcttg
    37621 atgaattcca catttatacg ttctacagat attttaccta atagctcgtg gttttctttg
    37681 atggcgtaga aggtttcttc ctctaatttg aaccctagtt gactgacaaa acgcaacccg
    37741 cgcatcattc gtaaggcatc ttcatggaac cgctcatgag gattgcctac agctcgcaag
    37801 atctgttctt ctagatcttg cagaccatcg aacaaatcga tgatttctcc atcttctttc
    37861 aaagcaaacg cattgatcgt aaaatctctt cttttcaaat cttctgccaa cgaacgaacg
    37921 aattcgacat gatctggtct acggtagtcc tgataagttg attccgtccg aaatgtggtg
    37981 atctcatatt gttcatcatt ttctaatacc aacacagttc catgttcgat cccaatatct
    38041 atcgtttttg gaaaaagctg tttgatttct tctggaaatg cgcttgttgc gatatccaca
    38101 tcatggattg gctggttcag caagatatca cgaacacttc cacctacaaa ataagcttca
    38161 tatcctgcat tctctaattt tttcagaact ggaattgcct tttggtattc cagaggtaat
    38221 tgttttaatt tcatttttcc aacctttcct gcgtgtttct tgtcctgaga taaagactca
    38281 gttgcatggt gactgtttga caagtccgtt tcagagtctt caaggccagg accacttcat
    38341 cttgtttact cgtccttacg ctcaaagcga ttatggtctc tttgattgaa tttttccatg
    38401 tttttttcaa aaacttcagt taagtcgata ttcaatgaat tagccatgat catcgtaacg
    38461 aataaaacat caccaagttc ttctgctacc gagttaactg gttcagaagc ctttttgctt
    38521 ttttctccat aagtgtggtt gacttctctt gccaattcac ctacttcttc agttaatcga
    38581 gccatctgag ctaatggaga gaaatacccc accttgaatt gctggatata ttcatccact
    38641 tcctgctgca tactgtataa agaacgtttt ttatccatct ttttccctcc aaaactatct
    38701 taccaaaact ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa tttccatgtt
    38761 acatttagga aggaatttct aaatggaaat tctgtgaaaa tacataattg aaagctggaa
    38821 ttcatatggt caaacgaaaa acagtaaaag aagttctttt tatcattata ggaacaagta
    38881 tttatgcatt tggtcttgtt tacttgaaca tcgctaatca tttagcagaa gggggagtct
    38941 cagggatcac cttgatctta agagctttat ttggtattga tccagcttat acgactttat
    39001 taatcaacat cccattgatt ttactaggag gaaagatcct cggtaaacgt tccttggcct
    39061 atactgttct aggtacactt tctttgtcat tctttttatg gatctggcaa agggttccac
    39121 tagaaatcaa tttacagcat gatctactga tcgtttctct acttgcgggt ttagttgcag
    39181 gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac aggcggcagt gatattatcg
    39241 ctcggatttt agaacaaaat tacggcatca ctatggggcg ttccctattg gcatttgata
    39301 tcatcgtctt acttgcttca ttaacctata tcgacttgaa acgtatgatg tatacgttga
    39361 ttgtttcgta tgttttcagt cgtgtgattg attcgatctt agatggcgga tattctgcaa
    39421 aaggtctact tgtcgtctcc aacaaaagcg aagagattgc accactactg atgaccggtc
    39481 tgcaaagagg tgtgactttc ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa
    39541 tcatctatat ggtcgttagc tctagtgaat taactgaagc taagaggatc gtccacgaga
    39601 ttgacgaaaa agctttcctt tcaatcatca atgtgcatga agtggaaggg caaggcttta
    39661 cttatttgaa gccacagaca agattactga aaaaactata agaaaaaaga caaagatagg
    39721 ctatgaggaa agattccaca tccagctgac ctttgtcttt tttagtacta tactattttt
    39781 aactaattgt tatgttcact tacccggtta cttttctttt gtgtctgcaa aaggatcttt
    39841 cggttaactt catcagataa tttcaaaaaa gcatccctat ctccatcttc tagtgcttga
    39901 tcgatttgag cataaagcat ttttagtttt gcctcttctt ctttttcttg aaaatatcgt
    39961 tcaattaatt cagtatcttc ttcactgact gaagaatccc aagaagaaaa tggattgtct
    40021 tctaagatag atagatattc tcttgtctgc caagcatttt cgaatataca ttccacatat
    40081 aaaggctttt tccaattcaa acggatttca tgaaaaatct ggtctgtgtc cgtaaattct
    40141 tttccctgca aaaacaattt catgggttcc ccttcaacag atagatcact gatctgcagt
    40201 cctcgatccg caaaattcgc cccttcaaca aagtggacat tgcttaaaat cgcttcatga
    40261 ttggcaagat aattcaagat ccaaaaaact tctcttcggc taaaagatac atgattgacc
    40321 atccacgtta aaaaattctt cttttcccgt acatcaatta gcatttttct cacctctctg
    40381 ttaggtcatc taataatgac agtgcctcca tatctcctgg ttcaagtgtt aaataataag
    40441 tcaataattc ttgcgctctt ttcagctgcc cttcttctct taagaaaaag gcatactctt
    40501 tcaagaaatc tggttcatgt gatagttcat gataagcctg ttcatagtgt acagctgcaa
    40561 cggtaaagtc ttctaattca ttataagcat gggctaggtt ccattctgca taaggattag
    40621 cagtttcttc catctgattg atcacttgta tcacatcttc atatctttca tcatccaagt
    40681 aaagattact taaagtaagt aatgtttcat cttgtttgtc tcctacttct aaagctttca
    40741 gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa agcattttcc gaggccaggt
    40801 gataaagttc gacttggtaa ggattttctt tgattccttc ttctaacact gtttgtgctt
    40861 cttcaatcat ttcttcttct tgaagagctt gtcctagata tagatataac gattgataat
    40921 gcggattaac ttctcgaagt tcttccagat agttgattgc ctgctgattt tctttcaatt
    40981 gaagattcgt aaaagctagc tggaacagtc gatcatctgt cctttcttct ttcagtgctt
    41041 cttctaaagg tacgatagct gcttcgaatt ctccttgcat acttaaggat tgtcctaaac
    41101 gttcctgcat cgagattcct gaaatttcat ttatcccgct tgccaacaat gtttcataaa
    41161 ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc gcaaactgga
    41221 tcaatggttc atcaggaagc aacccagctg cttctttcaa ttttgcttca ctcacttcag
    41281 ggattccgat aacttggtac aaatcagcta tcgccaataa gctttgcgga tagtaatcac
    41341 tttgcttatc cactttttcc aggtattcaa aagctaagtc gatctcatta tcttcgattg
    41401 caatttctgc taacgggatg ttcagtccgt cattttctgg gtattggttg atcagttgtt
    41461 caaaaatctg ttttgcttcg atcaaaaagc caatagccag caattcttcg cctagttcag
    41521 ctaatatatc tgctgggtct tcttttaatg ctttttctaa aaagatttgt gcttgtgaca
    41581 agtcttcatc tgataaagct tgcaacattt tttcgctatt tgtattcatt tcaattccct
    41641 cttatctcac tgacgatttg ttgaaatacc gtcattgttc tcaataattc tttttcgttt
    41701 ggatgttggt atccgtctat tatacctatt ttttctaaaa caagtatttt tttgcttttt
    41761 tctgccaaaa aaagaacttt ttccaaatac tccatctttg aaatctgttc cagccagtgg
    41821 attggatagc caagcaagtc cagccacaca aaaaagtttt ttagttggaa atcaaaagga
    41881 tatgcttgga gattccacac gaaatgaaac atcattccta aaaatttttt catactagca
    41941 gaaagtagat ggccattttc ggtcagataa aaagcttgtt caaacagttt accataattt
    42001 gacaggtttt ctgcctctaa ttgataaaaa tagatcattt cttcgatcat cccggcaaac
    42061 ggccgagtcg ttaattgctt tttatttgga tagtttttat acagattttg caaaaaacga
    42121 tgatcacaaa tcattccgca acagataaaa gtcaaaaagt caatctgctt tccgtccacc
    42181 tgccgctcgc tgattgtttg atcataaacg atgcgttctg gcagattgat ggtttgcagc
    42241 actacacgat ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa
    42301 cggatcgata cagggatcat ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa
    42361 cctgccagat ccatgatccc ttcatttcca aatccgatga taaacatgga ttgattttcg
    42421 ggatagcgtt tgacgaaatc aagcaggttc tttaattctg ttaaatgatt gcattgagtg
    42481 ttggcacaaa tataccagtc attgtttgct tgatttgcaa ataacgggtt gatcttatct
    42541 gcaaacaaat cataatagcg ctgattcgtt aaaaataaga ttggctgatc aatggaaagt
    42601 tctcttagct gactgactag cgtttctccg taaaacactt tcgtcaacgc atcttctact
    42661 tgataatcaa gttccatacg catccctcct cttcttattt ttattttacc attagtaagc
    42721 aaattctaga aaagataaga gcaaagctca cctcataact tgcaaataaa taaaaattaa
    42781 ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt ctcttattta
    42841 ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt
    42901 tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa tgcaggtact
    42961 ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg
    43021 cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc gttagctaaa
    43081 gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt tttagttaaa
    43141 tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga tagattcacc
    43201 tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag tggtccagaa
    43261 gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca tatgaaccgc
    43321 ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa acgtaacaaa
    43381 cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc
    43441 tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg atttgatttt
    43501 ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa acgacaaatg
    43561 taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt cctttatctg
    43621 ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca gccgaagaga
    43681 tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt tccaaccgtt
    43741 gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg aattcatcac
    43801 gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga tcccatttat
    43861 tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact cgcttgtctt
    43921 gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa cggtcaatgg
    43981 cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt ccgcgtttac
    44041 gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg aagtgggtgt
    44101 cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct tctcctaaaa
    44161 tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta aacttaatcg
    44221 tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca tctaaaatat
    44281 caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc cctaaggaat
    44341 aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact gctaaaatca
    44401 ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct gtcacgcctt
    44461 cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt tctgcttgat
    44521 gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc
    44581 tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt actccaggag
    44641 tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta gacttaccga
    44701 cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct cctcacaagt
    44761 ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga ttgtcaatta
    44821 aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa aaactgagac
    44881 aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc agaatcatcg
    44941 gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc catagtaaat
    45001 ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc ttctgaagct
    45061 ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg atggatctct
    45121 aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt cgcaatatgt
    45181 ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac ttcaacaaag
    45241 gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac tggagctttt
    45301 gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga aacacgttct
    45361 tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc caatacatca
    45421 cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc gtcaattccg
    45481 cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact gagaacatct
    45541 ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt ttccactacc
    45601 gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat tttaaagctc
    45661 aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac cattgatgca
    45721 gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac atttgtaaca
    45781 ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca aactttttta
    45841 gcatctaaac ggcgttttga aagtagatag ctgccgtttt ctttatcttt gccgatcgta
    45901 ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat atcttctacc
    45961 ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc ttcgattccg
    46021 acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc gccaactttt
    46081 acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg attttgatca
    46141 aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg gtactgtttt
    46201 tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca
    46261 attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc aaaacaacag
    46321 cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata cgaacagcat
    46381 cctctgcttg cttcagcgga gagacttccc ttgttgaatc ataatggtct ctttcctgta
    46441 tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc tctttgaaac
    46501 gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct gcattcggta
    46561 atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc cctatttttc
    46621 gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca gaaacagctt
    46681 ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg aatacatgct
    46741 gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa actagccctt
    46801 cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg cgatacatgg
    46861 caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca acggtacttt
    46921 tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata taactccttt
    46981 ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat tcttgaccag
    47041 ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga tcagtggtga
    47101 tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc gttgttgaac
    47161 cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt
    47221 gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg
    47281 ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat tctactgttg
    47341 aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact gatgtcgaag
    47401 tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc cagaaataag
    47461 cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa agaaataatg
    47521 tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca tctgtatcgt
    47581 agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga tgtctatctt
    47641 ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata ggaaaaagtg
    47701 tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta aaatcgtttc
    47761 ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc
    47821 tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa aataagctaa
    47881 gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct gcaggcggaa
    47941 ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt tttcagactg
    48001 ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct ctgtggcata
    48061 ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa agaaataatg
    48121 gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct gatctctgcc
    48181 tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat aatggaccac
    48241 ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa tgagaaactg
    48301 cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat caagaccacc
    48361 gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt cgacttcttt
    48421 ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc
    48481 ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat tgactgattg
    48541 tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg ctactaaatc
    48601 tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc ggatttttcc
    48661 taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat gagcctcatc
    48721 tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag gattagtcaa
    48781 catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga cgtatcttct
    48841 ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc gtttttgtaa
    48901 ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga ccagaccttc
    48961 cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag gcaaaatagc
    49021 gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg gacgaaatga
    49081 atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct tcctttctca
    49141 atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct acattccatt
    49201 cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg attgtttctg
    49261 cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca ataagatgat
    49321 ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt gtctgaccat
    49381 tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt aataatgaat
    49441 cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga
    49501 tacatctgct ttgttgcaga taaaccgctg actcatcatt gttttttgcc agctgataag
    49561 gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct gctgcttctt
    49621 ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc aaaatttcac
    49681 gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa ggacttgtct
    49741 ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga acagcaaatt
    49801 taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat
    49861 gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt gcttcatttt
    49921 catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa aacttatcct
    49981 gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat ccataaataa
    50041 gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc cgcagcttgt
    50101 gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta agttaggatg
    50161 gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat gcggtctttg
    50221 tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt tatttgccca
    50281 agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg taaaaaaagc
    50341 gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa tataaacgaa
    50401 actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc tgtttctctt
    50461 gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt taattgacta
    50521 gttttttcac ggaccctata ggacccattt atattatttc aactttttca gccagttttc
    50581 caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct ctccatctcc
    50641 tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg atacatactc
    50701 tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca atactttatc
    50761 ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc cttttttgtc
    50821 gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat tcttattgac
    50881 ataacgactt gccattacgc cacctaatga atggccaccg atcacataat cggaatttgg
    50941 aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat caagtggcat
    51001 tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa tactataact
    51061 ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc catgattctt
    51121 tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta ccaacacttg
    51181 cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg ctccaccgag
    51241 taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca ctttctccca
    51301 ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct taaaaaaata
    51361 agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc atttgaatat
    51421 ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc ttctgcataa
    51481 atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg gtgcataaag
    51541 tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt acgagcaaaa
    51601 gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg atatgtcccc
    51661 aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag gaagtcaaat
    51721 tcttcataag tcaacccata tttttctgct gttttagtta atttagctac taaatcatct
    51781 ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc tggtgtgatt
    51841 tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt tttgcccacg
    51901 acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat tgggccaaaa
    51961 tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc ttgtgctgca
    52021 tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc ttcgaaatcg
    52081 aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga gtaaggagct
    52141 gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt cgttcctggt
    52201 cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa ttcagcatct
    52261 ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa acaacgccat
    52321 aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc cacgccagca
    52381 tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc atcttgggat
    52441 ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt ttcccaatta
    52501 ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa ttcatctcca
    52561 gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct aaatcctagc
    52621 ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc gaagggatgt
    52681 ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc gctcaatgat
    52741 tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt atcctcctaa
    52801 tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc aaataatgac
    52861 gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca
    52921 ctcactgaat ggagctattg acttatccac gtgcttatcg atagccgttt atcggtttct
    52981 tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt gctgctacac
    53041 ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta aatttccacc
    53101 acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca gatgttggtg
    53161 taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa tccttaccga
    53221 cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct aagccggaag
    53281 ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg atgaatacac
    53341 ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc
    53401 cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct acaccgatca
    53461 aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg ccataagtga
    53521 ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag taccagtcac
    53581 ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt gttaaccagt
    53641 ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga atcaaactga
    53701 tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt tttttcgtga
    53761 ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct ttacgttggt
    53821 tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga tacacatata
    53881 caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata ccttcaccca
    53941 tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc gttgaagcta
    54001 gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta tcaaagccta
    54061 cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct gccattccgt
    54121 aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt ttttcttttc
    54181 ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt ttgttgataa
    54241 tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca gcaggtgttt
    54301 ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc atgaaaatat
    54361 cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg atgttgcctg
    54421 catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg attactgcaa
    54481 taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt
    54541 ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta atgttatttt
    54601 atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt ttgttcaaaa
    54661 agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc ttctttttca
    54721 ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg agaagaattt
    54781 cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact caaaacaaac
    54841 aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa agaccaagaa
    54901 atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga actgatgttg
    54961 ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag cactcacgat
    55021 gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg ctaaagacat
    55081 gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg taataacgaa
    55141 ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga cacctaaaaa
    55201 tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg ggaatgtaaa
    55261 gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag aaaaacctgt
    55321 agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg ctggaccgaa
    55381 taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa aactaaaagc
    55441 cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa acatcgctac
    55501 tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta ggactcctct
    55561 tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca gggaaaatag
    55621 agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat ccagactata
    55681 ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt cgtggactat
    55741 aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa tttttgttta
    55801 catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata aaaagtaagt
    55861 gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc acggtaatcc
    55921 ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag tttttgcaca
    55981 ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc gtggatcgtc
    56041 aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa tttagctgga
    56101 gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat cgattttgta
    56161 gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg atgtgtcagg
    56221 cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc gtaatccagt
    56281 cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac aacttttctt
    56341 cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa atagtaaccg
    56401 tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt cccaactttt
    56461 atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag gatgaattct
    56521 tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa tctctccatt
    56581 acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg tcttcttcaa
    56641 gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa ccaaaataat
    56701 ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct actctacctt
    56761 tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc tgaactcctc
    56821 gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt gctaaggttt
    56881 cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta agtaagggag
    56941 ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata tgcaaagctg
    57001 agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg acttctgctt
    57061 cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg ccaacaataa
    57121 aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac tgctggataa
    57181 agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt tttcatcaat
    57241 tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc aaaaaaagaa
    57301 tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac cattcgttct
    57361 tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa tttcttacgt
    57421 tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc tcgcttcaac
    57481 ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta ataaagactt
    57541 cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg gtattctaaa
    57601 agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc gtctgtgacg
    57661 aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc agccatcact
    57721 aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata attcatatat
    57781 tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg ctgaatcaga
    57841 tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat ctccgtcaac
    57901 tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc tctgtgattt
    57961 cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac atttgaaagc
    58021 ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc agctgatcgt
    58081 tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct ggcgtaaaga
    58141 aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac aaagaagttt
    58201 tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg ttttctttcc
    58261 ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa tgctgcttat
    58321 aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta gagatatccg
    58381 cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc agatgtgtgg
    58441 caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc tctcgtacta
    58501 actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga ttattgacaa
    58561 atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct aagtagcgtt
    58621 ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct ccttttccaa
    58681 ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca atcaattcac
    58741 tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt atccctaacg
    58801 tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc aatgtacttg
    58861 gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga tcagtgatac
    58921 gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga gtaacagttg
    58981 ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa aagcgatcga
    59041 catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt tccaaatcac
    59101 gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag tagtggagat
    59161 aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat atcagatacc
    59221 gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc tagacactca
    59281 gacggactgg tttctttttt ctttttcata gcattcctga cagattccat ggaacgttag
    59341 ccgatgatct tttactaaaa aatgataacg ttcttctact acttgctcca cttcaagtaa
    59401 aagatcctct tctacttcct cgatcgtgcc gcagttcaag catagtaaat gatgatggaa
    59461 atgtttggcg ccttcttttc tcaaatcata acgggctaat ccatcattaa aacttacttt
    59521 atcgacaatt ttcaattctg tcaagatttc aagggttcga taaacagtag caagtccaat
    59581 ttccggactc ttttttttca ctaaaaagaa gatttcttct gcagataaat gctctttctc
    59641 attttccaaa agcacaagca gagtagcttc tctttgcggt gtcagcttaa accccgcttc
    59701 atgtagctgc tgttttgttt ttttcatcgc cgaaatagca ttcatgacca tttactcaac
    59761 cccaacaatt tataataatt acaaataaaa aaagatataa cactatgtta attaaaatta
    59821 ttataagcta gttaagaaaa acatgcaagg gattctcaat tagatttttt gactagtttt
    59881 gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta
    59941 aatgctcctt tgctgatacc aaaagctttc atgatttctt ctggatccga tttatctgta
    60001 aagttgattt gctgatctgc agaacgttct aaaatggtta aaatcatttg tgcatcatct
    60061 gatatagctt catatgctct tggcttcaat gaaagattca gtacaccgtc tggtcggaca
    60121 ccaatcactc gaccttttat ctgctcgcct agacgaggtt cattatatcg ttcagaagga
    60181 tggataaacc caagatagaa atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg
    60241 tatgctgttc ctatcacatc tttgtttttc atttcttctg ttccatattt gcttaaagat
    60301 ttgaatattt tttcatctgc taaactgccc caaatacggt cttttgcatc aactctaagg
    60361 gaaatcatca agcgatcccc ttttttaggc catagctctg tcattgtcgg caattcatca
    60421 agagatacga caaagtcttt atctggaaga ccgatatcga caaacgcacc taaatctttt
    60481 cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta
    60541 gtgatccgat tttcatgttt ttgattttgg taaccgaatc cttcaactgc ttcaccgatt
    60601 ttgtgttcac cttctgattt gcttaagcca aaggtttgtc cgtttttttg gacaaagtag
    60661 tttttttcat tttcatcaat aataatacct gtaaatatag ttgctaataa atcgttcatt
    60721 tttttctccc tctatagtga aaggttttct taatacattt ctttgctatc ataacataat
    60781 gttatctatt tgaatagctg agccttggtt tggaaaggtg aaaaaaggag tatgacaaca
    60841 gttgtgtcat actccccttt ttcgaataaa cggtgttaaa cggcaggctc acagcctctt
    60901 tcatttgcct aaaattaatt gctcccattt cctccgaacc atccagtttt acagttgatg
    60961 gttacgatgt aaaatggcaa agcactttct ccaatgcctt tcatcaatgc agattttgtt
    61021 gaagtggcat atagattata atagccttcc ccattttcga ttctggcttt ttctaatctg
    61081 taacctcctt caacgatata tccacgttgg atacatgtag caatcacttt gctttggaca
    61141 cctggtgccc acgtccatgc tggattagat gcatcggcaa tatcacttgc tctggcagat
    61201 ttgattggct gattcgctga gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt
    61261 gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc ttgagcggct
    61321 tgttttgctt gttcttcagc tgcttttttc gcttcttctg cttttttctt ttcctcttct
    61381 tcttttttct ctttatcagt gagcatctta tctacttgtt tcaattgttc attcaactga
    61441 gcagatagat ctgtattttt cactttttct actgcttctt ttgccgtctt gtattggtcg
    61501 cgtgttgctg aatccactac ttttccgtct ttaaagacta tagctgtttt ttctttagct
    61561 gtttgcaatt cagcatattg atttttagct tctgtttgtg cttgttgaat caattttcca
    61621 aaatctgtgt tatccgtttc gattgcttgg atctctttat ctgctttcaa cttgatgtca
    61681 tctgctagat gatcatcgac gatggcaggt gctatgaacc actcattcac tttttggata
    61741 tctcggatct tagattggat atcttggtaa gttgtttcta actctgaata tcctttttca
    61801 ttcttaagac tggataaatc attttttagt tttgataaat cttgatgaat catatctgca
    61861 cgaataaatt gatggtgatc atctgtataa aaggcagcaa gtttctcatg aagttcttcc
    61921 atttgttgct gtctttttgc ttctgctgct acttcggctt ctactttttg atgatggtca
    61981 taataagccc agcttcctcc accgagtaga agtactgctg ctaaaacgag ataaggtact
    62041 ttttttgatt ttttcttttg cgctttatct ggatctgttt tttctaagtt tgattcttga
    62101 tgtgtttttt cagtatctgg cacgattgtt attgatttcg cttctgcttc aaacagctta
    62161 ggctggttct cttctttttc tatttttgct tcaagatcag taccctcttt gcttacagat
    62221 tgattttcag catcggattc actatttatt tcatcgggct ctgttgactt tttgtctgtg
    62281 ttttcgagta tttctttttt ctctgcttcc ggcagtttat tcgttttttc cttttctaat
    62341 tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc ttttattttt
    62401 actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc ttgttccttt
    62461 tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag cagctctttc
    62521 gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag tatcgaattt
    62581 tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg ttttgattca
    62641 agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt tgctttcttc
    62701 tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc gtcttctggt
    62761 tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa ttccgaccat
    62821 tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga tttatcctgc
    62881 atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc ttctgcagtt
    62941 atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga tccgcaattt
    63001 ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta ccatttaatc
    63061 tatcatattc tatacgtcac gccaatagc
//
LOCUS       DACCWK010000003        61652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCWK010000003 DACCWK010000000
VERSION     DACCWK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.003"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(87..464)
                     /locus_tag="IVW65_000177"
     CDS             complement(87..464)
                     /locus_tag="IVW65_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899111.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            720..1448
                     /locus_tag="IVW65_000178"
     CDS             720..1448
                     /locus_tag="IVW65_000178"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7899112.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1448..1702
                     /gene="purS"
                     /locus_tag="IVW65_000179"
     CDS             1448..1702
                     /gene="purS"
                     /locus_tag="IVW65_000179"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7899113.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1704..2375
                     /gene="purQ"
                     /locus_tag="IVW65_000180"
     CDS             1704..2375
                     /gene="purQ"
                     /locus_tag="IVW65_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7899114.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2376..4598
                     /gene="purL"
                     /locus_tag="IVW65_000181"
     CDS             2376..4598
                     /gene="purL"
                     /locus_tag="IVW65_000181"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7899115.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4583..6022
                     /locus_tag="IVW65_000182"
     CDS             4583..6022
                     /locus_tag="IVW65_000182"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7899116.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6054..7097
                     /locus_tag="IVW65_000183"
     CDS             6054..7097
                     /locus_tag="IVW65_000183"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7899117.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7094..7672
                     /gene="purN"
                     /locus_tag="IVW65_000184"
     CDS             7094..7672
                     /gene="purN"
                     /locus_tag="IVW65_000184"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7899118.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7669..9204
                     /gene="purH"
                     /locus_tag="IVW65_000185"
     CDS             7669..9204
                     /gene="purH"
                     /locus_tag="IVW65_000185"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7899119.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9220..10494
                     /gene="purD"
                     /locus_tag="IVW65_000186"
     CDS             9220..10494
                     /gene="purD"
                     /locus_tag="IVW65_000186"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7899120.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10616..11530
                     /locus_tag="IVW65_000187"
     CDS             10616..11530
                     /locus_tag="IVW65_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7899121.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11663..13228)
                     /locus_tag="IVW65_000188"
     CDS             complement(11663..13228)
                     /locus_tag="IVW65_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7899122.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13221..14672)
                     /locus_tag="IVW65_000189"
     CDS             complement(13221..14672)
                     /locus_tag="IVW65_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7899123.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14686..15702)
                     /locus_tag="IVW65_000190"
     CDS             complement(14686..15702)
                     /locus_tag="IVW65_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7899124.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15922..17010
                     /locus_tag="IVW65_000191"
     CDS             15922..17010
                     /locus_tag="IVW65_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7899125.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17064..18590
                     /locus_tag="IVW65_000192"
     CDS             17064..18590
                     /locus_tag="IVW65_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7899126.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18592..19797
                     /locus_tag="IVW65_000193"
     CDS             18592..19797
                     /locus_tag="IVW65_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7899127.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20054..21031
                     /gene="guaC"
                     /locus_tag="IVW65_000194"
     CDS             20054..21031
                     /gene="guaC"
                     /locus_tag="IVW65_000194"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7899128.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21295..22719
                     /locus_tag="IVW65_000195"
     CDS             21295..22719
                     /locus_tag="IVW65_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7899129.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23180..24289
                     /gene="pdhA"
                     /locus_tag="IVW65_000196"
     CDS             23180..24289
                     /gene="pdhA"
                     /locus_tag="IVW65_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7899130.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24292..25269
                     /locus_tag="IVW65_000197"
     CDS             24292..25269
                     /locus_tag="IVW65_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7899131.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25294..26937
                     /locus_tag="IVW65_000198"
     CDS             25294..26937
                     /locus_tag="IVW65_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP7899132.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26945..28351
                     /gene="lpdA"
                     /locus_tag="IVW65_000199"
     CDS             26945..28351
                     /gene="lpdA"
                     /locus_tag="IVW65_000199"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7899133.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28513..28788
                     /locus_tag="IVW65_000200"
     CDS             28513..28788
                     /locus_tag="IVW65_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7899134.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28794..29561
                     /locus_tag="IVW65_000201"
     CDS             28794..29561
                     /locus_tag="IVW65_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7899135.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29729..31558
                     /gene="typA"
                     /locus_tag="IVW65_000202"
     CDS             29729..31558
                     /gene="typA"
                     /locus_tag="IVW65_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7899136.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31819..33591
                     /gene="recQ"
                     /locus_tag="IVW65_000203"
     CDS             31819..33591
                     /gene="recQ"
                     /locus_tag="IVW65_000203"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7899137.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33705..34007
                     /locus_tag="IVW65_000204"
     CDS             33705..34007
                     /locus_tag="IVW65_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7899138.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34010..35173
                     /locus_tag="IVW65_000205"
     CDS             34010..35173
                     /locus_tag="IVW65_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7899139.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35199..38627
                     /locus_tag="IVW65_000206"
     CDS             35199..38627
                     /locus_tag="IVW65_000206"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7899140.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38648..39772
                     /locus_tag="IVW65_000207"
     CDS             38648..39772
                     /locus_tag="IVW65_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899141.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39762..40199
                     /locus_tag="IVW65_000208"
     CDS             39762..40199
                     /locus_tag="IVW65_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7899142.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40183..40521
                     /locus_tag="IVW65_000209"
     CDS             40183..40521
                     /locus_tag="IVW65_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7899143.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40640..41200
                     /gene="rsmD"
                     /locus_tag="IVW65_000210"
     CDS             40640..41200
                     /gene="rsmD"
                     /locus_tag="IVW65_000210"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7899144.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41197..41688
                     /gene="coaD"
                     /locus_tag="IVW65_000211"
     CDS             41197..41688
                     /gene="coaD"
                     /locus_tag="IVW65_000211"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7899145.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41678..42721
                     /locus_tag="IVW65_000212"
     CDS             41678..42721
                     /locus_tag="IVW65_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7899146.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42880..43608
                     /locus_tag="IVW65_000213"
     CDS             42880..43608
                     /locus_tag="IVW65_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7899147.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43724..43969
                     /locus_tag="IVW65_000214"
     CDS             43724..43969
                     /locus_tag="IVW65_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7899148.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43966..44184
                     /locus_tag="IVW65_000215"
     CDS             43966..44184
                     /locus_tag="IVW65_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7899149.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44320..44859
                     /locus_tag="IVW65_000216"
     CDS             44320..44859
                     /locus_tag="IVW65_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7899150.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44852..47125
                     /locus_tag="IVW65_000217"
     CDS             44852..47125
                     /locus_tag="IVW65_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7899151.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47163..48209
                     /gene="holA"
                     /locus_tag="IVW65_000218"
     CDS             47163..48209
                     /gene="holA"
                     /locus_tag="IVW65_000218"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7899152.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48298..48552)
                     /locus_tag="IVW65_000219"
     CDS             complement(48298..48552)
                     /locus_tag="IVW65_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7899153.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48952..49221)
                     /locus_tag="IVW65_000220"
     CDS             complement(48952..49221)
                     /locus_tag="IVW65_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899154.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49691..50146)
                     /locus_tag="IVW65_000221"
     CDS             complement(49691..50146)
                     /locus_tag="IVW65_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7899155.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50284..52212)
                     /locus_tag="IVW65_000222"
     CDS             complement(50284..52212)
                     /locus_tag="IVW65_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7899156.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52409..53242)
                     /locus_tag="IVW65_000223"
     CDS             complement(52409..53242)
                     /locus_tag="IVW65_000223"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7899157.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53434..54138)
                     /locus_tag="IVW65_000224"
     CDS             complement(53434..54138)
                     /locus_tag="IVW65_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7899158.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54161..54787)
                     /locus_tag="IVW65_000225"
     CDS             complement(54161..54787)
                     /locus_tag="IVW65_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7899159.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55113..57488
                     /locus_tag="IVW65_000226"
     CDS             55113..57488
                     /locus_tag="IVW65_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7899160.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57512..58003)
                     /locus_tag="IVW65_000227"
     CDS             complement(57512..58003)
                     /locus_tag="IVW65_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7899161.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58190..59110
                     /locus_tag="IVW65_000228"
     CDS             58190..59110
                     /locus_tag="IVW65_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7899162.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59264..59608
                     /locus_tag="IVW65_000229"
     CDS             59264..59608
                     /locus_tag="IVW65_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7899163.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59798..60298)
                     /locus_tag="IVW65_000230"
     CDS             complement(59798..60298)
                     /locus_tag="IVW65_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7899164.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60826..60909
                     /locus_tag="IVW65_000231"
     tRNA            60826..60909
                     /locus_tag="IVW65_000231"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60860..60862,aa:Leu,seq:cag)
     gene            complement(61233..>61652)
                     /locus_tag="IVW65_000232"
     CDS             complement(61233..>61652)
                     /locus_tag="IVW65_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7899165.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTET
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20224 a        10651 c        13204 g        17573 t
ORIGIN      
        1 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
       61 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
      121 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
      181 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
      241 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
      301 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
      361 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
      421 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
      481 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
      541 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
      601 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
      661 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
      721 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
      781 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
      841 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
      901 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
      961 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
     1021 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
     1081 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
     1141 aacttgcaaa gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
     1201 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
     1261 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
     1321 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
     1381 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
     1441 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
     1501 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
     1561 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
     1621 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
     1681 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
     1741 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
     1801 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
     1861 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
     1921 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
     1981 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
     2041 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
     2101 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
     2161 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
     2221 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
     2281 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
     2341 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
     2401 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
     2461 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
     2521 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
     2581 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
     2641 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
     2701 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
     2761 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
     2821 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
     2881 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
     2941 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
     3001 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
     3061 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
     3121 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
     3181 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
     3241 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
     3301 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
     3361 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
     3421 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
     3481 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
     3541 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
     3601 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
     3661 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
     3721 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
     3781 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
     3841 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
     3901 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
     3961 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
     4021 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
     4081 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
     4141 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
     4201 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
     4261 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
     4321 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
     4381 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
     4441 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
     4501 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
     4561 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
     4621 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
     4681 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
     4741 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
     4801 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
     4861 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
     4921 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
     4981 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
     5041 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
     5101 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
     5161 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
     5221 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
     5281 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
     5341 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
     5401 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
     5461 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
     5521 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
     5581 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
     5641 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
     5701 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
     5761 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
     5821 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
     5881 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
     5941 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
     6001 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
     6061 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
     6121 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
     6181 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
     6241 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
     6301 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
     6361 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
     6421 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
     6481 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
     6541 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
     6601 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
     6661 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
     6721 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
     6781 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
     6841 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
     6901 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
     6961 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
     7021 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
     7081 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
     7141 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
     7201 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
     7261 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
     7321 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
     7381 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
     7441 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
     7501 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
     7561 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
     7621 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
     7681 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
     7741 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
     7801 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
     7861 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
     7921 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
     7981 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
     8041 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
     8101 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
     8161 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
     8221 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
     8281 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
     8341 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
     8401 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
     8461 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
     8521 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
     8581 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
     8641 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
     8701 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
     8761 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
     8821 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagtgttt
     8881 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
     8941 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
     9001 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
     9061 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
     9121 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
     9181 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
     9241 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
     9301 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
     9361 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
     9421 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
     9481 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
     9541 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
     9601 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
     9661 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
     9721 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
     9781 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
     9841 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
     9901 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
     9961 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    10021 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    10081 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    10141 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    10201 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    10261 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    10321 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    10381 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    10441 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    10501 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    10561 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    10621 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    10681 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    10741 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    10801 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    10861 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    10921 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    10981 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    11041 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    11101 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    11161 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    11221 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    11281 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    11341 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    11401 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    11461 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    11521 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    11581 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    11641 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    11701 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    11761 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    11821 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    11881 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    11941 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    12001 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    12061 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    12121 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    12181 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    12241 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    12301 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    12361 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    12421 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    12481 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    12541 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    12601 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    12661 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    12721 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    12781 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    12841 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    12901 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    12961 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    13021 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    13081 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    13141 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    13201 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    13261 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    13321 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    13381 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    13441 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg
    13501 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    13561 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    13621 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    13681 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    13741 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    13801 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    13861 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    13921 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    13981 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    14041 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    14101 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    14161 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    14221 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    14281 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    14341 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gctctctcga
    14401 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    14461 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    14521 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    14581 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    14641 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    14701 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    14761 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    14821 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    14881 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    14941 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    15001 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    15061 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    15121 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    15181 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    15241 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    15301 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    15361 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    15421 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    15481 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    15541 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    15601 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    15661 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    15721 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    15781 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    15841 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    15901 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    15961 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    16021 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    16081 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    16141 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    16201 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    16261 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    16321 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    16381 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    16441 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    16501 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    16561 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    16621 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    16681 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    16741 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    16801 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    16861 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    16921 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    16981 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    17041 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    17101 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    17161 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    17221 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    17281 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    17341 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    17401 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    17461 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    17521 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    17581 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    17641 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    17701 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    17761 agcatattat tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    17821 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    17881 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    17941 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    18001 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    18061 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    18121 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    18181 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    18241 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    18301 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    18361 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    18421 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    18481 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    18541 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    18601 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    18661 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    18721 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    18781 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    18841 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    18901 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    18961 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    19021 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    19081 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    19141 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    19201 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    19261 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    19321 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    19381 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    19441 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    19501 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    19561 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    19621 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    19681 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    19741 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    19801 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    19861 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    19921 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    19981 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    20041 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    20101 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    20161 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    20221 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    20281 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    20341 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    20401 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    20461 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    20521 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    20581 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    20641 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    20701 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    20761 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    20821 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    20881 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    20941 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    21001 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    21061 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    21121 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    21181 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    21241 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    21301 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    21361 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    21421 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    21481 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    21541 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    21601 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    21661 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    21721 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    21781 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    21841 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    21901 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    21961 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    22021 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    22081 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    22141 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    22201 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    22261 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    22321 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    22381 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    22441 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    22501 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    22561 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    22621 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    22681 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    22741 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    22801 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    22861 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    22921 taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg
    22981 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    23041 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    23101 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    23161 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    23221 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    23281 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    23341 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    23401 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    23461 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    23521 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    23581 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    23641 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    23701 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    23761 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    23821 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    23881 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    23941 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    24001 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    24061 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    24121 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    24181 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgagg tatctccaca
    24241 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    24301 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    24361 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    24421 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    24481 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    24541 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    24601 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    24661 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    24721 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    24781 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    24841 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    24901 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    24961 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    25021 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    25081 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    25141 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    25201 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    25261 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat
    25321 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    25381 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaatcccg
    25441 tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt agcaaatgtt
    25501 ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg ggacagtggt
    25561 gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt tgatacagaa
    25621 tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat cggtgaagga
    25681 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    25741 gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca
    25801 gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta
    25861 gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa
    25921 gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca
    25981 aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat
    26041 atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac
    26101 ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca
    26161 ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag
    26221 tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct
    26281 aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa
    26341 gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg
    26401 aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca
    26461 gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat
    26521 atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac
    26581 cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac
    26641 ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct
    26701 gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta
    26761 ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg
    26821 atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca
    26881 atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa
    26941 acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc
    27001 tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga
    27061 aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat
    27121 agctgcagga caccattatc aagagtcact agattctgaa atgtttggag taactagtga
    27181 aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac
    27241 gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga
    27301 agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta
    27361 ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa
    27421 attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa
    27481 atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt
    27541 aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga
    27601 tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc
    27661 aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg
    27721 gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac
    27781 agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt
    27841 ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg
    27901 ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg
    27961 caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt
    28021 ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata
    28081 taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat
    28141 ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg
    28201 cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga
    28261 tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga
    28321 attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga
    28381 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
    28441 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
    28501 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
    28561 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
    28621 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
    28681 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
    28741 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
    28801 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
    28861 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
    28921 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
    28981 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
    29041 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
    29101 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
    29161 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
    29221 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
    29281 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
    29341 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
    29401 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
    29461 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
    29521 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
    29581 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
    29641 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
    29701 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
    29761 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
    29821 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
    29881 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
    29941 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
    30001 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
    30061 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
    30121 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
    30181 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
    30241 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
    30301 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
    30361 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
    30421 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
    30481 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
    30541 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
    30601 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
    30661 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
    30721 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
    30781 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
    30841 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
    30901 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
    30961 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
    31021 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
    31081 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
    31141 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
    31201 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
    31261 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
    31321 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
    31381 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
    31441 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
    31501 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
    31561 tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt
    31621 ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc
    31681 gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa
    31741 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
    31801 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggcta tgatgagttt
    31861 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
    31921 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
    31981 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
    32041 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
    32101 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
    32161 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
    32221 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
    32281 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
    32341 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
    32401 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
    32461 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
    32521 agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc cgtgggtaga
    32581 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
    32641 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
    32701 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
    32761 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
    32821 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
    32881 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
    32941 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
    33001 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
    33061 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
    33121 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
    33181 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
    33241 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
    33301 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
    33361 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
    33421 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
    33481 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
    33541 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
    33601 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
    33661 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
    33721 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
    33781 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
    33841 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
    33901 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
    33961 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
    34021 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
    34081 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
    34141 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
    34201 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
    34261 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
    34321 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
    34381 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
    34441 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
    34501 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
    34561 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
    34621 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
    34681 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
    34741 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
    34801 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
    34861 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
    34921 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
    34981 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg
    35041 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
    35101 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
    35161 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
    35221 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
    35281 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
    35341 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
    35401 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
    35461 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
    35521 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
    35581 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
    35641 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
    35701 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
    35761 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
    35821 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
    35881 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
    35941 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
    36001 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
    36061 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
    36121 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
    36181 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
    36241 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
    36301 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
    36361 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
    36421 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
    36481 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
    36541 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
    36601 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
    36661 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
    36721 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
    36781 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
    36841 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
    36901 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
    36961 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
    37021 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
    37081 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
    37141 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
    37201 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
    37261 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
    37321 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
    37381 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
    37441 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
    37501 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
    37561 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
    37621 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
    37681 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
    37741 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
    37801 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
    37861 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
    37921 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
    37981 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
    38041 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
    38101 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
    38161 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
    38221 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
    38281 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
    38341 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    38401 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    38461 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    38521 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    38581 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    38641 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    38701 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    38761 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    38821 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    38881 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    38941 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    39001 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    39061 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    39121 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    39181 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    39241 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    39301 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    39361 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    39421 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    39481 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    39541 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    39601 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    39661 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    39721 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    39781 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    39841 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    39901 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    39961 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    40021 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    40081 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    40141 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    40201 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    40261 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    40321 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    40381 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    40441 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    40501 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    40561 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    40621 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    40681 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    40741 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    40801 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    40861 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    40921 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    40981 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    41041 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    41101 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    41161 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    41221 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    41281 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    41341 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    41401 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    41461 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    41521 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    41581 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    41641 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    41701 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    41761 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    41821 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    41881 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    41941 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    42001 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    42061 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    42121 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    42181 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    42241 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    42301 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    42361 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    42421 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    42481 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    42541 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    42601 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    42661 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    42721 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    42781 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    42841 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    42901 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    42961 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    43021 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    43081 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    43141 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    43201 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    43261 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    43321 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    43381 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    43441 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    43501 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    43561 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    43621 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    43681 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    43741 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    43801 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    43861 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    43921 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    43981 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    44041 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    44101 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    44161 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    44221 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    44281 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    44341 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    44401 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    44461 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    44521 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    44581 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    44641 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    44701 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    44761 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    44821 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    44881 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    44941 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    45001 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    45061 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    45121 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    45181 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    45241 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    45301 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    45361 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    45421 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    45481 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    45541 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    45601 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    45661 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    45721 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    45781 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    45841 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    45901 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    45961 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    46021 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    46081 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    46141 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    46201 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    46261 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    46321 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    46381 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    46441 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    46501 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    46561 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    46621 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    46681 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    46741 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    46801 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    46861 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    46921 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    46981 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    47041 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    47101 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    47161 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    47221 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    47281 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    47341 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    47401 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    47461 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    47521 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    47581 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    47641 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    47701 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    47761 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    47821 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    47881 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    47941 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    48001 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    48061 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    48121 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    48181 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    48241 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    48301 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    48361 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    48421 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    48481 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    48541 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    48601 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    48661 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    48721 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
    48781 tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga
    48841 gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc
    48901 tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg
    48961 tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa
    49021 ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg
    49081 taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag
    49141 ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa
    49201 aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg
    49261 cactaattct tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca
    49321 ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt
    49381 ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc
    49441 ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa
    49501 cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc
    49561 tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc
    49621 tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac
    49681 aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag
    49741 caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa
    49801 caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga
    49861 cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta
    49921 atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga
    49981 tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg
    50041 gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta
    50101 aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc
    50161 actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct
    50221 tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt
    50281 tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga
    50341 agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat
    50401 tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga
    50461 agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg
    50521 catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac
    50581 gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc
    50641 ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag
    50701 ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg
    50761 ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat
    50821 acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg
    50881 aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt
    50941 tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt
    51001 ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa
    51061 tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc
    51121 gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag
    51181 aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc
    51241 tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa
    51301 atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga
    51361 agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc
    51421 tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag
    51481 aggaacagca agcaaaatca attttacata attattttcg attcgttcga taaatgaagc
    51541 tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt
    51601 ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc
    51661 atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc
    51721 atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc
    51781 gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt
    51841 ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg
    51901 acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat
    51961 tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa
    52021 atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata
    52081 tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc
    52141 gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt
    52201 ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta
    52261 attgataata ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg
    52321 atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc
    52381 tcaaataaga gaagtgttct ggttttttct acattgcaat aaaccaataa ccaatcaaaa
    52441 tgataccaag gataatacga taccagccaa aagcggtaaa gtcgtttttc ttcaaatagc
    52501 ttagtaagaa tttgatagct aagacggaaa ccacaaaaga tacgaatgtt cctacaagaa
    52561 gaacaacagt ttcttccatt ttgaatgaat tcccttcagc taaatatttg acgatcttca
    52621 agccgcttgc gccaaacatg acaggaatcc ctaagaaaaa tgaaaactct gtagcgacaa
    52681 aacgcgaagc accaatcaag atagatccta ggatcgttgc gccagaacgg gatgttccgg
    52741 gaacaagtgc caagacttgg aataacccaa caagaagagc tgcttggtac gtaaagtcat
    52801 tcagagaagt ccaacgaggt gttttgtttt tgttgcgttt ttcgacaata ataaaggcaa
    52861 taccgtaaac gatcaaaaca atggcaactg gtaagaactt gtggaaattt gcttctaacc
    52921 aatcatccaa aggcaaacca atgatgatgg ctggtatgca agcaacgatc actttaaacc
    52981 aaagtgtcca cgtatctttt ttttctaccg ttgatttact tggagaaaat gggtttaact
    53041 tgtgaaaata aagaacgacg acagccaaga tcgcccctaa ttggataacg acattgaaca
    53101 tcgacataaa ttgtgaactg gcatccagtt tgatgaattc atctgctagg atcaaatgac
    53161 cagtactact gatgggcagc cattccgtta ttccttcaat aataccgaga atcactgctt
    53221 tgataatatt tgcaaaaaac ataaattcct tccttcccaa ttaagataca tcttatagta
    53281 taccgttatt ttttgagaaa cccaatctgt tttttaatat taagaaaaac ttgagaaata
    53341 aagcagataa gtacacgaag agaaaaaaga agacaaagcc ctcgttcaga ctttgccttt
    53401 tatgattctc tggtaaaaat taaaatatcc ccattataga aacgagtcgc gactatgtgt
    53461 tttcccgtta tcgatataag acgtatcatt gatgccgtgg atagaaaatc gaccgtttgc
    53521 ataagtgacc tttgttacgc tgccatttaa aagcccagca cgaacaggct gtttaggatc
    53581 gatcagatta agcaaaaagg cgatcgtcaa accatgagat acgactaaaa catttccacc
    53641 accatttttt tcgcagtgat gggcgatatc ttcaaatcct gtccatacac ggtctcgcaa
    53701 tgtttcataa gtttccgccc acccagcggt atctaagttg tatacagcat tagcaatttg
    53761 ttcaaaagaa acttctgtgg taaacatttc atcatatgtt ttaaaattca atacacgagg
    53821 gatcacaccc cacatttcca tgtcatatcc accttcaaga gacccgaaac accattctct
    53881 aatccttttt tcaataaaat aaggaatttc ttttccctta gaatgttctc ctagaataat
    53941 acgggcagtt tcgattgcac gaccactgtc actagaataa gcaaatgaaa agtcgacatt
    54001 ttttaacccg cgacctaaat aatgaatgcc ttcttgtcct tgctttgtga ggggtgtatc
    54061 acaccagcct tgtactcgtt caattgtatt gaacatcgtt tttccgtgtc gtacgatata
    54121 aagtgtagtt tctgtcattt tattctcctc ctaagtcgtt ttaacgattg aaaaaaggat
    54181 ttgtcctttt ttcctgttca atcgtcgttg catcgccatg tcctggatag actgcaaatt
    54241 catcaggtaa tacaaacagc tgggtccgaa tactatgaag taattgttct aaattccctg
    54301 tatacagatc ggttctaccg atacttcctc taaacagggc gtcgccagaa acgacaaaat
    54361 catcaaaaat aaagctgacg cttcctatag aatgccctgg ggtagggacg acgcgaaaag
    54421 acatgtttcc taaagtataa gttttcattt caaattcata ttctgcaggc gagacaataa
    54481 catcagcaat atcatcatgt ctaccgagtc cagaaagatt caaaatcgga tcacctagcc
    54541 atttttgctc tagtgggctg acataaactg ggatttgata atacgcgcgt aaaggttcca
    54601 ctgcaccgat atgatcgtag tgagtatggg taagcaaaat cgctaccggc tgttggtttg
    54661 tttttttgat ttcttgtttg attcgttccg catcttctcc agggtcaatg atcaaaagtg
    54721 cttcttcgtt gtagaccaaa taacagtttt cttcaatcat tcctgttttt aatctttcga
    54781 tatgcatgtg cattcctcct tgatagccaa aacttctttc atccattagt ataacgcatt
    54841 cattgctagg aaacaaaaac aggtacttat ttctctttac ttatctgaat tatcagacta
    54901 tatgaaaagt atttggatat tctaaagaga aggaaagtaa gttttcatag cattttaata
    54961 tgtccttgtt acattagatt catccaaaaa tccaaattct atatcctata tccctgccta
    55021 atcagcaggg tgttttttta tgtttggaaa gagagctctt tttgctaaac taggtttttt
    55081 caggtaatct tagagagaaa gaggtggtgg aaatgatcaa acagtcaaaa atatctgtgc
    55141 ggcacttagt agaatttatt ttgcgacgtg gaagtatcga caatcgtaaa aaaagtaacc
    55201 atactgcatt agaaggagcg agaatccacc gcaagctcca aaaagatgcg ggagaaaact
    55261 atgaaaaaga agtcttttta aaaacaacag ttcaattaga tgaatattac ctgatcgttg
    55321 aaggccgtgc agacggtatt ttcaaaagag acggtattta ttatatagat gaaatcaaaa
    55381 cctccgaacc acgatttgaa gatttagaac cagaacaagt ggaacttttc tttcatcaag
    55441 caagggtgta tgcctatatc tatagtcaag aacatgattt acaagaaata aatctgcaat
    55501 tgacttactt ccagacggca gaagaagtga tcacgagaaa ggttgaacat cagacaagag
    55561 agcagttaga aacttttttt aaaaagctga ctgaagatta taaagaatgg cttgtttttc
    55621 aagaaaactg gcgcactgta cgcaatgcct ctttgatggc tttgaaattc ccccatgatg
    55681 agtataggaa aggtcagcgt gaactagcgg tagctgctta taaaacgatc cgtacgaaac
    55741 agaaattgtt tgtagaagca cctaccggaa caggaaaaac aatctctacg ttattccccg
    55801 ctttgaaagc tgtaggggaa gaagagggag aaaagatctt ttatctaacg gctaaaacaa
    55861 taactagaca agtagctgaa gatgctatga cagcattaaa agatacagga gctgaagtaa
    55921 aaagtgtcac tttgacggcg aaagacaaga tctgcttttt agatgagacg acctgtaatc
    55981 cagatcagtg cccatatgcc aatggttatt acaatcggat taatgaaggt ttatgggatc
    56041 ttttgaatca tgagaatcag atcactcgtg aagtcatcga aacttatgca agaaagcata
    56101 cactttgtcc tttcgaacta tcattagatg tcagtatctg gtgtgacgta atcattgggg
    56161 attacaatta tttatttgat ccaactgttt atttaagacg tttttttgaa gatgaaaaaa
    56221 acgaggacta tctgtttttg attgacgaag cacataattt ggtgaatcgt tcccgagaga
    56281 tgtattcggc agaactatct tatgaaaaaa cgaaacgaag taaggaagct ataccaaagg
    56341 aattcaaaaa acttcatcgc cgtttcaaca aactattaaa agaatttgat tcgatccgag
    56401 aaatagcaaa agaagatcat tgggattatc atcatcaaaa agcaccggct gaatctttag
    56461 taaaggcagg ttatcaatta agcgaaaaaa ttaaagaatg gctggcagaa tttcctgagc
    56521 acccatcaca agaacaattg cttccttatt attttgattt gcttcatttt ttgaaggtca
    56581 gtgaatttta tgatgatcac tacgaaacaa cagtcgaaaa aacgtatcgc gatctgatag
    56641 taaaagagtt ttgtattgat ccaagcttgt ttcttgagca gtcgttggat aaaggaaaaa
    56701 gcagtattct tttttctgct agtttttcac cactctctta ttatcaggaa acactggggg
    56761 gacaaaaaag tctggcttac aaactgccga gtcctttccc agaaggaaac cagcaagttt
    56821 tcatcgcaaa ttacctagag acgacttatc gaaaaaggga acacagtctg gcaaaattag
    56881 tagagacgat tcatgctttt gctgatggaa aggtcgggaa ttatttcgtt ttctttcctt
    56941 cttatcaata tttagatctt gcggtggaag cattcagaca gcaatatccc gacagcaatg
    57001 tgctaatcca agaaacggac atgaatgagg aagaacgcga acgattttta acaaaatttc
    57061 aaacaaatcc cgctgaaaca ttgattggat tttgcgtact tggcgggatt ttttcagaag
    57121 gaatcgattt gagaggggac cgattgatcg gcagtatgat cgtaggtgta ggtttgccgc
    57181 agatgaatca tgagcaggaa ctgatcaaat catattatga tgagaaggaa caccttggat
    57241 ttgcttacgc ttatcagctt cctggaatga acaaagtctt gcaagctggt ggtcgagtga
    57301 ttcgtggcat ggcggatcaa ggaatcgttg tattggcaga tcaacgattt tctagttttt
    57361 cttatcgacg tttattccca caacattggc aaacagcaag agaagtacga agtgttgggg
    57421 aattggaaga aggaatacag cgtttttggt tatcaaaaga agcagtaaat caaaaaaacg
    57481 aggaatgaca gacgtcagcc tcgttttttg attaacgata gttagtgaat tgcaactcga
    57541 ttggtaagtc aagtttacgt aataaagcaa tcacagcttg tagatcatca cggttcttac
    57601 ctgtcactct aatctgatct tcttggattt gtgcttttac tttcagtttt tcatttttga
    57661 tagctgtagt gattttcttc gcgttttcac gatcgattcc gttgaccaaa tcggcagttt
    57721 gtcgagcttt gccacctaga gcgtgttctg attcggaaaa atggatattt ttgattggca
    57781 catttctttt gatcagctta ccaaataata catctttgat ctgttcgatt ttgaaatcat
    57841 catcgctcaa tacaactaga ttgctgttct ctaatttgat ttctactgta gaatccttga
    57901 agtcaaagcg attggacaat tcttttgttg tttgctgaat cgcatttttt acttcttctg
    57961 tattgacttc tgatacaatg tcgaagctag catcttttgc cataggaacc ctcctgatat
    58021 acatataaat atttaccata catctatctt atcataggat tagggcagat tcttacctgt
    58081 ttgctaaaat tcttctcatt tgagttcgaa gtattttatt ttaatagata aactgttatt
    58141 cttacatttg gaataccaaa gtaggggaga ggtgcttgtg agtaaaatta tgactgcgta
    58201 tcataaaaat gaaagattaa agaaaatagt ggttattttg ataaccggat tgaccgctgc
    58261 ggtcgcttta aattattttt tgattccagc caatgtcttt tctgcaggga tgaatgggat
    58321 cgctcaaatc atttcctcgc ttttatatga atggtttcat atcaagatag acacaggaac
    58381 atttattttt cttttgaata tccctgtgtt tgtattaggg tttctcaaag taggaaaacg
    58441 agcaacgatc cttagttttg tgaatgttgt atgtgtatcg gtgatgacga tgctgcttcc
    58501 agtcggacag atcactgata atattttgat gaatgcccta gtaggcggtg ttttattagg
    58561 tcttggcgta ggattatctt taaaaatggg ctttacaact ggtgggatgg atatcatctc
    58621 tcttgtactt tcacaaacaa ctggaaaaac agtcggaaac tacatgctga ttctaaatgg
    58681 tatcattgta gtcatcgcag gcttcttctt taactgggaa agtgccttgt atacgattat
    58741 ttccatttat gcaatgactc aagtaattga tgccatccac acgagtcatc aaaaagtaac
    58801 agcaatgatc gttaccgtca agccagaaga ggtaacagaa gaactagcaa aacggatggt
    58861 acgtggaatg acattactgc cgtctgtcgg tggctattca aaaaaacaag ggaacatgat
    58921 catgatggtc atcacccgct acgaaatgta tgatcttgat cagatcgttc atgaaatcga
    58981 cgacgatgca tttatcaata ttttaccaac tcattcagta tttggccggt ttgcaaatga
    59041 aaacgaacaa aaaatgtatc gaacaactgg cgtattgcca gaaataaaaa atcgttcaca
    59101 aaaaaaatga ggaatcaggt ttattttaga ataaatttaa tctattacat ctaataagaa
    59161 catttttata aatttaaacg agaatcttac ttctaaattc tcatttacaa gcagctaaaa
    59221 aaagagtatg ataaataaaa atcgtgttaa ggagtgtcgt acgatgagcg aagcaaacca
    59281 agaacggtct ggacaagaaa tagaactaat caaagaacgt attcttgctg ccttggaaat
    59341 ggtgattgat cctgaattag gaatcgacat cgtcaattta ggcttgattt atgatataga
    59401 attcaatcct gaaaatggag aaacagtcat taagatgact ctaacaacta tgggctgtcc
    59461 attagcagat attttaacag aatctatcca tgacgcatta aaagaagtgc cagaagtaac
    59521 gaaagcagaa gtcaaactag tctggtatcc agcatggacg acagataaga tgagccgtta
    59581 tgcacggatt gcgttgggga tacgatagaa atcttggctg actagctatg agaagcaaat
    59641 aggaaaaaca aaaaagcctt ttatcctttc tagaagaaaa gagataaaag gtttctttgt
    59701 ttataaaatt ttgtctgcta tattataagt taattaaata aatgtgttct attgataaag
    59761 gacattcaaa tccctttcct tttggaatcc ttctttttta catcagtttg attttcgtat
    59821 agtattcttt ctcatcttca cgataaatcg cagccccatt tttttgttgt gctttaagac
    59881 tgccaagatt ttttttattt acagacaaaa gtgcctcatc aatgccgatt ttttttgcct
    59941 cttgtagtac taatgctaat ccttgtgtgg catatccttt tttacgataa ttaggggaga
    60001 tgccgtaacc aatatggcca gcgccgtttc ttaatgtttc gttcagttta tggcgcaaat
    60061 taaaaattcc tacatactca tgttcatcac tttctaaaat aaagacggtt gcgggaacgt
    60121 aactctcagg aagatcgatt cctaaagacg ttttttcgca tttttttaca taatctaaaa
    60181 attcagcaaa tgtatagcca taagcattgt tgataaatcc actctcattt ggtccaaagc
    60241 tagtttgtag ctgataagct ttctttaaat ctgcttccca tagtttgatc agtttcatcg
    60301 tattcacctc tttaagtaac cttattcatt tgttttctac tatagcacta ttgttgtaaa
    60361 tagccatgcc tatttttatt tattgtgtca tatcgtatta agaatcatcg aacaatacaa
    60421 atatgaatta caataaaaaa attatttgaa gttcgttcat tcgttactta tgatctctag
    60481 aagaaagaaa acgtgaatta cttcagcgtc tagaattcca gaaggatagt aatcaacaga
    60541 agaaataatc aatatccaaa tgaaagaaga tagctgaatc gagaaaaaga ggaaaaaaga
    60601 aatgaagaaa aaaataaaat agttactaat cccagagtcg ttcgtttaac attgaaatcg
    60661 atcatatgga taaaacagat tttttcacca accctcaata gatgatggag ttgctcggat
    60721 gaattatttg tgtagatggc ataagggagg gcgtacagaa atgaaaaaga acaaaaatat
    60781 gttcttgaca aaaaacacat gtaatcctaa actaaaagat gtgatgcggc tatggcggaa
    60841 ttggcagacg cgctagcttc aggcgctagt gagggtgacc tcgtggaagt tcgagtcttc
    60901 ttagccgcat aatttgtttt tttataaaga gaaggtacac ctggagcgat gagaaagaaa
    60961 ataacatatt atctaaaaga atataggaaa aattttaaac aacggaacca gattcaaata
    61021 aatcaaaaaa aatttgtgtg aaggtgggac agtttaaaac ctaatagatt gattcaaata
    61081 gtagatttat agattctgat acaagaaata ctggcaaacg atagttaagg aataaagact
    61141 aaacttaaac tcaaaaagag gtcatgcgaa agaataaatc gcatgacctc ttactatatt
    61201 ttgaatgtgt ttaaacgttc aaataatgaa tcttagtacc aaccgtttgc ttcccagaat
    61261 gctttcgccg cttcccaaga accataacgt gaagtaacat attgttctgc tactctttct
    61321 tggtttgcag cagagtagtc accatttaag taagaagaat ctaattggta acggccgatg
    61381 taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat ccattctttt
    61441 gcagagcttg tacttgctgg agtagcagca gtttctgtta cagcaggagc aggttgtgtt
    61501 tcagcaacag gcgctgcttg tgtttccgtt tgaaccgttt cttgtacaac aggttgttca
    61561 gcttgaactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61621 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DACCWK010000004        56575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCWK010000004 DACCWK010000000
VERSION     DACCWK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.004"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(592..1983)
                     /locus_tag="IVW65_000233"
     CDS             complement(592..1983)
                     /locus_tag="IVW65_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7899166.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2196..2582)
                     /locus_tag="IVW65_000234"
     CDS             complement(2196..2582)
                     /locus_tag="IVW65_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7899167.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2638..3261)
                     /locus_tag="IVW65_000235"
     CDS             complement(2638..3261)
                     /locus_tag="IVW65_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7899168.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3325..3993)
                     /locus_tag="IVW65_000236"
     CDS             complement(3325..3993)
                     /locus_tag="IVW65_000236"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7899169.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4005..4445)
                     /locus_tag="IVW65_000237"
     CDS             complement(4005..4445)
                     /locus_tag="IVW65_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899170.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4504..5174)
                     /locus_tag="IVW65_000238"
                     /pseudo
     CDS             complement(4504..5174)
                     /locus_tag="IVW65_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5359..5757)
                     /locus_tag="IVW65_000239"
     CDS             complement(5359..5757)
                     /locus_tag="IVW65_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7899171.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5754..6525)
                     /locus_tag="IVW65_000240"
                     /pseudo
     CDS             complement(5754..6525)
                     /locus_tag="IVW65_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            complement(6522..7205)
                     /locus_tag="IVW65_000241"
     CDS             complement(6522..7205)
                     /locus_tag="IVW65_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7899172.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7372..8259)
                     /locus_tag="IVW65_000242"
     CDS             complement(7372..8259)
                     /locus_tag="IVW65_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7899173.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8256..9125)
                     /locus_tag="IVW65_000243"
     CDS             complement(8256..9125)
                     /locus_tag="IVW65_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7899174.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9262..10548)
                     /locus_tag="IVW65_000244"
     CDS             complement(9262..10548)
                     /locus_tag="IVW65_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7899175.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10821..11399
                     /locus_tag="IVW65_000245"
     CDS             10821..11399
                     /locus_tag="IVW65_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7899176.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11399..12328
                     /locus_tag="IVW65_000246"
     CDS             11399..12328
                     /locus_tag="IVW65_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7899177.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12325..14061
                     /locus_tag="IVW65_000247"
     CDS             12325..14061
                     /locus_tag="IVW65_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7899178.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14124..14960)
                     /gene="pheA"
                     /locus_tag="IVW65_000248"
     CDS             complement(14124..14960)
                     /gene="pheA"
                     /locus_tag="IVW65_000248"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7899179.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14957..15466)
                     /locus_tag="IVW65_000249"
     CDS             complement(14957..15466)
                     /locus_tag="IVW65_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7899180.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15459..16754)
                     /gene="aroA"
                     /locus_tag="IVW65_000250"
     CDS             complement(15459..16754)
                     /gene="aroA"
                     /locus_tag="IVW65_000250"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7899181.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16767..17858)
                     /locus_tag="IVW65_000251"
     CDS             complement(16767..17858)
                     /locus_tag="IVW65_000251"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7899182.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17875..19041)
                     /gene="aroC"
                     /locus_tag="IVW65_000252"
     CDS             complement(17875..19041)
                     /gene="aroC"
                     /locus_tag="IVW65_000252"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7899183.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19043..20107)
                     /gene="aroB"
                     /locus_tag="IVW65_000253"
     CDS             complement(19043..20107)
                     /gene="aroB"
                     /locus_tag="IVW65_000253"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7899184.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20140..21162)
                     /gene="aroF"
                     /locus_tag="IVW65_000254"
     CDS             complement(20140..21162)
                     /gene="aroF"
                     /locus_tag="IVW65_000254"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7899185.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21821..22795)
                     /locus_tag="IVW65_000255"
     CDS             complement(21821..22795)
                     /locus_tag="IVW65_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7899186.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22776..24659)
                     /locus_tag="IVW65_000256"
     CDS             complement(22776..24659)
                     /locus_tag="IVW65_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7899187.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24677..26902)
                     /locus_tag="IVW65_000257"
     CDS             complement(24677..26902)
                     /locus_tag="IVW65_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7899188.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            27017..28051
                     /locus_tag="IVW65_000258"
     CDS             27017..28051
                     /locus_tag="IVW65_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7899189.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(28118..29131)
                     /locus_tag="IVW65_000259"
     CDS             complement(28118..29131)
                     /locus_tag="IVW65_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7899190.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(29209..29397)
                     /locus_tag="IVW65_000260"
     CDS             complement(29209..29397)
                     /locus_tag="IVW65_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7899191.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29837..29965)
                     /locus_tag="IVW65_000261"
     CDS             complement(29837..29965)
                     /locus_tag="IVW65_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7899192.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29968..31341)
                     /gene="rlmD"
                     /locus_tag="IVW65_000262"
     CDS             complement(29968..31341)
                     /gene="rlmD"
                     /locus_tag="IVW65_000262"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7899193.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31452..32507)
                     /locus_tag="IVW65_000263"
     CDS             complement(31452..32507)
                     /locus_tag="IVW65_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7899194.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32517..33947)
                     /gene="gatB"
                     /locus_tag="IVW65_000264"
     CDS             complement(32517..33947)
                     /gene="gatB"
                     /locus_tag="IVW65_000264"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7899195.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33944..35416)
                     /gene="gatA"
                     /locus_tag="IVW65_000265"
     CDS             complement(33944..35416)
                     /gene="gatA"
                     /locus_tag="IVW65_000265"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7899196.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35416..35721)
                     /gene="gatC"
                     /locus_tag="IVW65_000266"
     CDS             complement(35416..35721)
                     /gene="gatC"
                     /locus_tag="IVW65_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7899197.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35827..37863)
                     /gene="ligA"
                     /locus_tag="IVW65_000267"
     CDS             complement(35827..37863)
                     /gene="ligA"
                     /locus_tag="IVW65_000267"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7899198.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37880..40114)
                     /gene="pcrA"
                     /locus_tag="IVW65_000268"
     CDS             complement(37880..40114)
                     /gene="pcrA"
                     /locus_tag="IVW65_000268"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7899199.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            40269..41828
                     /locus_tag="IVW65_000269"
     CDS             40269..41828
                     /locus_tag="IVW65_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7899200.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41939..43870)
                     /locus_tag="IVW65_000270"
     CDS             complement(41939..43870)
                     /locus_tag="IVW65_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7899201.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43888..44802)
                     /gene="pfkB"
                     /locus_tag="IVW65_000271"
     CDS             complement(43888..44802)
                     /gene="pfkB"
                     /locus_tag="IVW65_000271"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7899202.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44799..45551)
                     /locus_tag="IVW65_000272"
     CDS             complement(44799..45551)
                     /locus_tag="IVW65_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7899203.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45710..45919)
                     /locus_tag="IVW65_000273"
     CDS             complement(45710..45919)
                     /locus_tag="IVW65_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899204.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45930..47258)
                     /locus_tag="IVW65_000274"
     CDS             complement(45930..47258)
                     /locus_tag="IVW65_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899205.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47623..48210)
                     /locus_tag="IVW65_000275"
     CDS             complement(47623..48210)
                     /locus_tag="IVW65_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7899206.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(48293..49543)
                     /gene="clpX"
                     /locus_tag="IVW65_000276"
     CDS             complement(48293..49543)
                     /gene="clpX"
                     /locus_tag="IVW65_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7899207.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49790..51079)
                     /locus_tag="IVW65_000277"
     CDS             complement(49790..51079)
                     /locus_tag="IVW65_000277"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7899208.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(51233..52165)
                     /locus_tag="IVW65_000278"
     CDS             complement(51233..52165)
                     /locus_tag="IVW65_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899209.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(52322..52981)
                     /locus_tag="IVW65_000279"
     CDS             complement(52322..52981)
                     /locus_tag="IVW65_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7899210.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52984..54009)
                     /gene="sppA"
                     /locus_tag="IVW65_000280"
     CDS             complement(52984..54009)
                     /gene="sppA"
                     /locus_tag="IVW65_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7899211.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(54022..54312)
                     /locus_tag="IVW65_000281"
     CDS             complement(54022..54312)
                     /locus_tag="IVW65_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899212.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54721..56526
                     /gene="glmS"
                     /locus_tag="IVW65_000282"
     CDS             54721..56526
                     /gene="glmS"
                     /locus_tag="IVW65_000282"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7899213.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT        15818 a        12178 c        10051 g        18528 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
      361 cttgcatctg cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg
      421 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag
      481 aagtagcttc tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa
      541 acttcttctt atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt
      601 cacatcgatc actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc
      661 acttgtcacg ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc
      721 gattaatggt tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc
      781 taaaacgatc agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact
      841 gattgttaca attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc
      901 tgctggtgcg acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc
      961 aatagacaaa ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt
     1021 tgtctgagtc tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa
     1081 gctcaatacc ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa
     1141 aatcagtgag tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt
     1201 acctaaattg acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa
     1261 tactccataa ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag
     1321 actgtcgact aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag
     1381 ataagcgatt ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact
     1441 acctgctaaa tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga
     1501 taagttttca actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc
     1561 tccttgtgtg aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa
     1621 tgctgcaata gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag
     1681 cttttcagaa actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat
     1741 aactagcatt tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga
     1801 ttgagtaacg actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat
     1861 accaactcct aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg
     1921 atagaacaca tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt
     1981 catttttctc ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga
     2041 gaatgattct tcaaccatcc tcctgaaaaa atttttttca gtgactttta tattttagac
     2101 gatttttatc gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg
     2161 atagctttaa tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa
     2221 ttcaatttta ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt
     2281 acgatatcga ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga
     2341 ccttcaattt tccgattgag gatatgattg atctgactca cacttccaga taaaacatat
     2401 gtatcaaaca ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag
     2461 ttttcgatcg tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg
     2521 ttcggcaatc tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc
     2581 atataatttt atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta
     2641 ctttttaatg atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc
     2701 cgggatctca cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt
     2761 ccctgcttcg atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg
     2821 aaaagtatcg atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc
     2881 gatccctact acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt
     2941 tgattctgtc aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc
     3001 cagtacttgt ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg
     3061 gtgaaataat tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt
     3121 agaatacttt ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt
     3181 tagtgcttgc catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa
     3241 gtcccatata taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct
     3301 ctcactttta gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc
     3361 tgctaatgta tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc
     3421 aggaacatct tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag
     3481 taaatcttgc cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg
     3541 attcactgtc atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata
     3601 tctgcggtca aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca
     3661 tgtttttact tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag
     3721 atccccataa tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc
     3781 catttcttct agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac
     3841 ggcatcgaat tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt
     3901 tcctttttct gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc
     3961 gctttcgcca tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg
     4021 aattttatac cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc
     4081 tgcttttgat gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact
     4141 tgctttttgt tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa
     4201 cgttgattct catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat
     4261 ccgaaaaaga cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa
     4321 ctaaagacaa gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt
     4381 ccctgcataa gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc
     4441 atcatattat ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa
     4501 gccttaattg gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc
     4561 aatcacaatc actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg
     4621 aagaaacagc ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat
     4681 aattgaggaa attgaataga cgtgtgaagc agacctggat attttttcaa cagctgtgtc
     4741 gttttgatca gattccgttt tttactgatc acttttaaaa agctttttag aatcttttgc
     4801 tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat ttgtttttgc
     4861 agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa accttctgga
     4921 aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata atcttttact
     4981 ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc tgcttgatag
     5041 atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag caatgtttta
     5101 ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc ttttaaaaca
     5161 actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca tattttgaaa
     5221 agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata agtattcacg
     5281 agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt tgtgtttatt
     5341 tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt catctaaaag
     5401 ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa tgactttgtc
     5461 tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc cttgaccaat
     5521 cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa tatctttttc
     5581 tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac ctcttccatg
     5641 taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat cttcgcccat
     5701 tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat ctttcatttg
     5761 ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc ttttcgttta
     5821 taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat tgcaaccaca
     5881 ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc cacgctcatt
     5941 gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc aaaagccatt
     6001 gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta tcctgggatc
     6061 aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc tactgctgcg
     6121 ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc ctcgctcaat
     6181 tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac taagaacccc
     6241 attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat agcactgcct
     6301 aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag gatcaaccca
     6361 atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg catacgaact
     6421 aatgatattc attaaaacaa ataggattac gataggtgcc actgccaaaa tgacttccat
     6481 ggtaacgatt tcgattccat ctatcacttg ctgccagaaa ctcattggaa gatcaccccc
     6541 aaaatcatca cggcaagaat cggccctaac gaagcaatac cgaccattcc aaaactgtca
     6601 ttttcatttt tcttttgttt gatcatacta gtcatccctc cggcaagggc caagataaat
     6661 ggaacagtta ctggaccagt agtcactccc cccgaatcaa aagcaatcgg cataaattcg
     6721 gaactggtga aaaatgaggc gatcaaaacg cctatgtagc ctatagccat caattgataa
     6781 taggatagct tgaatacgac ccgaagcagc gcaaaggcta gaaatactcc agtgccgacg
     6841 ctgacgatcc caatcagcaa cactctgcca atctctccgt cagatatatc atatacttgt
     6901 tgtcccaaaa cttgcacgga aggttcagca atcgtgatca cgatcccgat agcaaatccc
     6961 aaactgacca aaacggtcaa acttttcttt ttgatcatat atttccctac tagatctccg
     7021 acttccatca tagaataatc tgctccaaac aaaaagagcg tcattccgat catcatgatt
     7081 gccgcaccaa ccagaaatga agcgaggtct tctccttcta acggtgcaaa aacaaacgtt
     7141 aaaatgacaa tcaggacagt catcggcaat atagcagcaa tcacttcttt aaaatttttt
     7201 agcatgtatc ctcctccttt actctttaag tttaccttaa aatgaaagag ttttcgagca
     7261 caaacttaaa acgttatcat ttaatttttc tcaacataaa ataacacacg tcccaaaagt
     7321 atctttttac ttggaaaaat gactttgaag gaacgcgctt attgtgtttt tttattttac
     7381 agagccgatc attccttgaa cgaattgctt ttgcaaaaca aaaaatacga tggcagttgg
     7441 taaagtagcg atgactaatc cagccatgat cattccatag tctggtgtat aggaagcacc
     7501 catagcagat aaaacaagcg gaaccgtccg tttatcagga gattgcaaag ctactaaggg
     7561 ccataaataa ttgttccagc tactcatgaa tgtgatgatt gcagccgcgg cataggtatt
     7621 tttagccgtc ggcatataca ctctaaaaaa aatacttagt tcttttagac cgtctaatcg
     7681 agcggcttct actagatctt ttggaaaaga tttaacattt tgtcgaaaga agaagatcaa
     7741 aaaagcagtg ctaacagatg gcaggatgac aaccaagaaa ctgttgattc ccaaaaccgt
     7801 tccgttcaat tgtgaaaaca ttcgatataa agggatcatc aatgccgcaa aaggaaccat
     7861 catcgatagc agtagaatat tgaaaattag gtcttttcgt ttactttgat aaatctcaaa
     7921 tccatatcct gccattgacg aaatcagcaa tgcaaacaca gtggtcacca aagcgatcat
     7981 cgctgaattc caaagggaac gagtaaaatt cagctcattg gaaaacaaat tttgaaaatt
     8041 gatgatcaag ttactgccaa tctttatttt tcccgatgtg atatccacgg gtgtattcgt
     8101 cattcccaaa accatccaga caaaagggaa aatggaaata aatgatagga tgcttaatgc
     8161 tgtatacttt aatactgacc caattgtcga acgaagttct aatgcttgtt ttttgacgtt
     8221 ggggcgttcg attgtttctt tttctaccat atttttcatc gactttctcc tcctactttc
     8281 gtttggatta tcgacaatat cacaataaag gaaacaataa caaaggaaac tgctgccgca
     8341 tatccaaaat taggcgtgaa tttataactc aaattataga tgtattgaga tagggtggtc
     8401 gttgcatttg ctggtccccc gccagtaata ttttgtactt catcaaaaag ctgcaatgtc
     8461 ccgatcgtcg aagtaatgga agtaaacaaa atgattggtt ttaaatttgg gatcgtcact
     8521 ttgataaact gctgccattt agaagcaccg tcaatagatg ctgcttcata aatctccgaa
     8581 tcaatatttt gcatgcctga aaggaaaaaa atcatattgt atccagtcca acgccaagtg
     8641 atcgaaagaa tgatcagaac tctcgcccaa attggatgag tcaaccagcc aatcggtgtg
     8701 tcaatcatac caatgcccaa caacaagttg tttacaaggc cagattgaga aaatagactt
     8761 ttcatgatca gagaataact gaccatcgaa gtgatacatg gaagaaagat agcggttcga
     8821 aacaaccctt tatatttcaa ctttttatca tttaaaaggt tggaaataaa aagtgctaga
     8881 aacaacatga tcggtacttg tacaagtaaa tacaggaatg tattaaaaaa agctttttta
     8941 aaagttgcat ctgtcaacat ccgctgataa ttacctaacc cgttaaatgt catattgaca
     9001 gggggaccac tttgaaaaga cgtgatcaaa gccaaaacca tcggaataaa aaccatgatt
     9061 gatatcaaaa tgactggcaa gattaaaaaa gcatgcccgt ttttgaataa atctttctgc
     9121 ttcattcttt tccctccaat tctatcattg attatctaca tgaaaagaca agaaagacaa
     9181 tatctgtaag gcagacgtga tcttttttgt cttttcgttt gtctgatgaa aaagcgaaga
     9241 aattcacttt tttaccaccg atcaattagc caattgtgct tctacttgtt tttgtgtatc
     9301 tgctagtacc ttgtctgtct cttctccatt gatgattcga tgaagagctt cagctacgac
     9361 tgcttcaatt gcataggtct catgtccgta attcacttct ggtatttcag atgtccatgt
     9421 tgaaaaatcg tcaaagatct tttgaccact gtaaaattca gaagcttctt gataagcttc
     9481 ttgttcattc gcgctaagca tcgtagatac tagtccgatt tctttcgcaa gagtgcccat
     9541 cagttcttca ttcgaagcaa acgttgcttc taggaaatct ttcgctgaat cttctccagc
     9601 tactcctttg atcacatacc agccagcgcc gccaaggtta gaagcttgcg ctttttgcag
     9661 attttctgga agtgcaggta ttggtgcaat cttccatttt ccagattggt tttctgctcc
     9721 ttggatcgtt gaagaatacc aagcgccagt tgggctagaa gctactgctc ctgattgtat
     9781 cgcagttact ccagcattcc agtcagaggt ttgttcgact agatcttctt tcaaaagagt
     9841 ggcaaacaat tcaagaccat actttaaaga tgcattgtct tggatcgtga ctgtctcccc
     9901 atcttcagat gtataccatt ctcctgcctg ttgcataatc atccttactc gaccaaggtc
     9961 agaaggattg acttcagcga ttttctttcc cgtcttttct ttgacatctc gcgctacttg
    10021 aatatactcg tcccacttca gattctccat atcctcttct gtataacctg cttctgccat
    10081 cagatccgta cgatagaagt ttgctgttac gcccgaatcg aaaggaacac cgtatatttt
    10141 atctcctact ttgtttaccg caaatttata cggtgcaaaa tgatcttcct ttacaatagc
    10201 agacaaatta gcgaatgcat ctggataaga agtcaaatag ccttgtatcc tataatcttc
    10261 aatcaagacg atattgggca atccttctgt attcccgctc gctaaagcgg tgttcagttt
    10321 ctgaacgata tcatcctgag acatcgtaac aacttctatc tctgtttctt cgttctcata
    10381 aacttttttt gcttcattca ctgctttgat attgaatgtc tcatcccacg cccagacagt
    10441 gactttttct tctttgtctg ctgtactact agcacttccg catcctgtta agaaacctga
    10501 tgctgccgct aaacaaacga ctccagcaat taactttctg atgttcattt ttatccgctc
    10561 cttttccatc cattagttgt ttacgtttgc tacattcatc ataccaaaat ttgaaaacgg
    10621 tttctttatt attcagatac ttcaaaaatt tctcctaaaa taataaaaaa atacaaatca
    10681 actatgacaa aaggaataat cttagcatct agaccaaaga tcgaaatggt gaggtttctt
    10741 taaaataaaa atatccttgc attttctcct agaaataagt atttttaaat tacaagacta
    10801 ttcttaaggc gggtaaaaaa atgccaatac gcgtaattct tatcgatgat gaacagacga
    10861 ttttagatgg aatgaaacag ttgatcaatt ggtctgagca cgggtttcaa ttaataggga
    10921 ctttttccag tccgctgaaa gcgcttgatt tttctttggt acatgcaatc gatatcgtgg
    10981 tcacagatgt aatgatgcct gaattaaatg gaatcgaatt atcgcggcta ttaaaacagc
    11041 aacaacctga aacacagatc cttatcctct ctagctatga tgaatttgat atggttaaag
    11101 attcttttaa agaaggcgtc gctgattatc ttttaaagcc tcgattgagt cctgattttt
    11161 ttctaaattc cttaactgcc gtttcacaaa aaatcaaaaa aaagcccaac aaaagcccaa
    11221 tcaccaatcg ttatgagcag atctgcgaga atctttcaca gcagattgct ggacaaactc
    11281 gtccattgct tgaccctctt ctttttgaac acaccttgtt tttctgttat ataccgaaaa
    11341 acggccattc agttcaaaac aaggctctaa acaaaaacag tcgatcaccc attcattgat
    11401 ggatagcgac gattcgttta ctatttatcc tgtttctacc ttttcggaag aatccggcta
    11461 tgtaatcaat actgattcca aagatacact taggaagttt gttcaaaagt ttcgtcaaga
    11521 agtttttcca ggtgattttt ttgttgtttc tgattctttt gcaatccaag atatcaatga
    11581 aatattccaa gagatcagaa attgcaccaa aggacaacgt ttctatcata agggaaagaa
    11641 ccttgcttat cagcatgaac ttcttccgtt attaaccaac cacgagcagc aagcaagtag
    11701 ttatttaaca aaaatcgttc ataaagacta cgctttagct gtcaaagaac ttgagaattt
    11761 cttcacgacg atattggaac agcctattga tccagcttat ttgaaacatg aggcaatcaa
    11821 tcgtttctat gcattactca acgctttggc tgacgagcat gttgggaatg aagaactttc
    11881 tttgctcaag atcgcccttc cttctttatt agccgatgct gagtatttcg aagatttttc
    11941 tattcttgtc aaagaaacac ttgagaaact cacccacttt tctgcaaatg caaaaaaaga
    12001 caatttcgat atcttgactt tgatctacga gttcgtccag gaaaattatg agaaagaaat
    12061 caatttacaa cttttatccg aaaaatatca tttttcatat agctatctct cagccatctt
    12121 tactgaaaaa tttggtatca acttttcaaa atatctaaaa aaagtccgta tcagtaaagc
    12181 caagctcttt ttgacggaaa cacggcttac tttgacggaa atctgtgaga aagtcggcta
    12241 taccgaactg ggttatttct ccagagtttt taaagaagag acaggaatga caccttctca
    12301 ataccgtaaa ggaacttttg taaaatgaga aaaaaaatca aagagcatga actctttgcc
    12361 aaacttctgt tcattttgtt catcggtctt tttctgcaag ccatcgtcat ttctttgttt
    12421 atttatcatc gatcaaggga tgcctatatc cagctgttca atcaatcgaa tgatgtggta
    12481 ctaaaaaaga ttcaatctga atttgaaagt ttgaatgata caatcgagaa tacattagcc
    12541 gcttttgatt ctaatccagc tgtcaaaagc tatttttcca atgatccagc ccaacatatg
    12601 gaacagcttc agcaactaag aacaatccaa aaaatgaatg actctctttc aaaaatccat
    12661 ccaatgatcg attacgatgt attgatcttt ggagaaaatg gaagaacttt tgtcggaaac
    12721 gacatgctca ctgccgtatc tgccgattct ttttttcaat ctgcgattgc tcagagagtg
    12781 aatgaacgtg ctgctgatac acaaatgctt tttgcacacc acggattgac tcttcgcgat
    12841 aagaaagcac ccagcgtctt ctttgtcaaa aaactgaaaa atacattgaa tcacgtttac
    12901 ggatatgctg ttttatcgat ctcatccaag caactagcga atctttttca atctgtagta
    12961 aatcctgaga tatcgaagat ctccttcatg aacgaagaac agacgatcat cgcatcaaat
    13021 gaagaaaata caattgggag ccgatcaatg attttcaaaa aactaacagt aggtgaaaca
    13081 acgatttcaa atggacagca gttgacccgt cttcctctgt accgccagga ttgtacatta
    13141 ctcagtcaag tcgatcttca ttctttatct aaccaaatgg gcgtgatttt tccgattatt
    13201 ttgtataata tcgtgacact tgcttttgct ggctcattgg tatttatgta tttggatcga
    13261 cacacaaaat cgatctaccg cttgattgat tcgctaaaac ggctagagga agatggctta
    13321 aaagctactg ttcctgtaca aggaagccat gaagtccaat tgctagggaa tacgatcaat
    13381 caattgctaa gtaatgctca gcatcattat gaacagacga tccaagacga aaagaagaaa
    13441 cgttcgcttg aaatccaaac tatgcaagca cagatacagc cccattttat ttataatacg
    13501 ctgactagct tgaaattttt gatttggcag aaaaaaacag atgaagcagt tcaaggaatc
    13561 gacagtttca ttactttact aagaagtacg attgggaaaa aagaagaagt gattccagtc
    13621 aaggaagaaa tcaaaagcgt acaaagttac atcgatcttt tgagtatgcg atatggcgaa
    13681 agaatctcgg ctaaaataat gattcctgat gaactgcagt cattatctat gccgaatatg
    13741 atccttcaac caattatcga aaacgcctat cttcatgctt ttcacaataa aacgcaagga
    13801 ttcatcacgg tgtacggcaa aacaaaaaat cagcttcttt tattcgaagt gattgataac
    13861 ggcgatggct tcgatactga aaataccaaa caaaaaaatg atcatttttc aggaatcggc
    13921 atcagccatg tagatgagcg gatacgccta atgtatggcg atgcatatgg cttaacgatc
    13981 caatcagtga ttgggacagg aacgactgta acgatttgtc taccgcttct tcctaaaaat
    14041 caatcagaat atcaaaaata aagaatagcc aagcgctgat ttttttattt taagttaaat
    14101 aaatcaatca gtattcttga ctttcatcta cgcgtctctt ctactgtcag cactggataa
    14161 cagcccaata tttgactttc tcctccgagc atcttgattt cttggagcgc attctctact
    14221 aatgtcattg gacggtccaa caacaaatcg atcacgaaaa aatattctcc caagctagtt
    14281 ttcaatggtc gggattcaat cttacttaag ttgatttccc gccagccaaa agctgccagc
    14341 attttatgaa gcatccctgg ccgattagca ggtagtgtca agatgacact catttttact
    14401 ggagctgact gctggctggt catctttcgg tcaccgacga tccaaaaacg tgtctggttc
    14461 aactcattat cttggatatt ctcagctaag atctccaatc caacatgctg tgctgtttct
    14521 aatgaagcaa tcgctgctgc gtcttctttc ggatgttctg ctacatacat tgctgcagca
    14581 gttgttgatt ctgttgctac taaaggaaca ttaggataat gcgtttctaa aaactgttgt
    14641 gattgtgcta gtgcttgtgg atgtgaaagg atttttgtaa tctttgttgc tggattgcct
    14701 aaaagctgct gcttgatagg aagaacgatt tcacttttaa cttcgacttc tggatgtttg
    14761 gaaagaagat cgattgtgtg atgaacagct ccttccaatg aattctctac aggaacgaca
    14821 gccagatcga cttgtttgcg aaacaaagcg cgcaaacaaa cagggatgga agcatacgca
    14881 gtcaattgtt ctgtaggaaa tagctgacaa gccgcctgat aagtgaatga actttctggc
    14941 cctagatagc tgacgttcat atgcttttga tcctttctat aatggtatcg atgatctttt
    15001 ctggtgtttg attggtcgtg ttgattcgat gtgtcgcact ttcttcatac caagaaagtc
    15061 gttgtgccaa taatgctttt atttcttctc ttgtttttcc aagagctaaa ggtcgaatag
    15121 acgtttcatc ttgttggata cgatcaacta gacagtccgc ttctgcctct aagtaaagaa
    15181 caatcggctg tgtttttaaa aaacgccgat tttctgcact ttggacaatc cctccaccag
    15241 tcgcgataat tttgtcttca tttgacagct ttcttaaaat atctgactcc acttttcgaa
    15301 agcttttttc accgtaatag tgaaaatatt cggcaatcga caattggata tgttcctcaa
    15361 tctttgtatc taaatctaca tactcttttt gtaaacattg agctaaccct ttaccgattg
    15421 ttgtttttcc agcgcccatg aaaccaatca gcaaaatgct agccatgagc ttctcctcct
    15481 gacacaagtt ctgctaaatc gtcaaaaaat gccggataag agattgaaac agcttcagct
    15541 ccttctagtt cgcaaggtcc atcagctatc aaagcagcta cttgcagcat cataccgatt
    15601 cgatgatcgc ctctgctgga cacttttgcg ccatgcagag gtgtcggccc atggatgatc
    15661 agaccgtctg ctgtcgcttt tatctttgct cccattttct gtaattcttc tgccaccgca
    15721 tcaattctgt tggtttcttt tacttttaat tcttctgcgt ctttgatgat cgtgattcct
    15781 tctgcttgag tagccactaa agctagaatg ggcaattcat caatcagacg cggaatgatt
    15841 tctccaccaa tcgttgcttt cttcaaatga ctcgtcttga cacttaaatc agccgattgg
    15901 ttctgttcat tgtgattcgt ctgggtaatc gctgcgccca tctcctccaa cacatctaag
    15961 ataccagttc ttgttggatt gaccccgaca ttttttaaca acagttggct ttccggaaca
    16021 agaagtcctg ctgctagaaa gaaagcagct gatgaaatat cacctggaac agttatctgt
    16081 tgaccagtta atttttgcgg tccggtcact atgatcgttt tatcttccac tgtgatcctt
    16141 ccgccaaatt gacgaatcat ttcttccgta tggttacggg aacgttcttt ttcaacgatt
    16201 acggatgtgc cttctgcttg taatgccgca aacagcaaag cagatttgac ttgcgcactg
    16261 gctacaggca tattgtatcg gataggtgat aaagagcggg ttcctgtcac agtgattggt
    16321 agatactggt cattttcttc tccttgcaag tcagcgccca tctcgcgtaa aggttccatc
    16381 actcgcccca ttggacgttt agacaacgaa gcatccccga acagagtagt cgtaaaaggc
    16441 tgtccagcca aaatacccat caataaacgt atggtcgtac cggagtttcc catgtcaatt
    16501 gattcggttg gttggacaaa tgagtggcta ccgcgaccat gaatcttgat cacctcttcc
    16561 tcttcttcga tctcgacacc taattgttgg aaggcatgta aagtactcag acaatcttct
    16621 gcgcgcaaaa aattttgtat agtcgtcgtt ccttcagcaa ttgctccaaa catgatgctg
    16681 cgatgggaaa ttgatttatc tgctggtatc ctaacagtcc ctcttaatcc atgtatttgt
    16741 tgtaataatt gcatttatcc agcctcctat ttgatccagc aatgataact ggtattagac
    16801 ataatgattc tttttgcttg taacaaatcc tgttggtttt taaaactgat ttgcagtatt
    16861 cctaaaatat cttccctggt ctcttggatc ttcaaattaa tcaaagaaac tcccgctttt
    16921 ccgatcagtc cagttacttc tgcaataacg ccaggttcat ctggcacatc gataaataaa
    16981 tcaaaaaagg caggaatcgc gccttgctta tggacaggca actggtctcg actttctttt
    17041 gcccgattga aaaaagaaaa gatttccgat tggttctcag tcatgatcca ttctgctatt
    17101 tgtgtcattt cttgttgcca atctgccaat cgttccaata atatctgctt attgctcaac
    17161 aaaatatctg tccacatgac aggatctgag gaagcgatcc tagtgatatc tcgaaagcct
    17221 cctgctgcca gttgtttggc tcgcggatac tcttgattga attgttctgc ctgattgact
    17281 aagccagctg ccagaatatg tggaaaatga cttagcattc ctgttatacg gtcatgttct
    17341 cttgctgtaa gagttacata ttttgctttg gttccgcaaa atagttcttg cagtttttca
    17401 acatcttttt tcgtcttttc caaaggtgta aagatgtaat aggcattttc aaatagttta
    17461 gcatctgctg cctgtatacc tgatttatga gaaccagcca ttggatgtcc accgacaaac
    17521 cgaagatttt tctgctcagc ggatgtcacg acttgctgct tcgtactgct gacatccgta
    17581 atgatgacgt gatcagacaa gttgattctt tcaaggacat caagataagc taaggttgta
    17641 cggacgggaa cggctaataa gataacgtct gcttgttctg cacctgtttc aaaatcactt
    17701 gcaacgtaat caacgatctg ctgttctact gcttgctcac gggtttttga ctgactatcc
    17761 caaccaacta ctatcagttc tggattggtc tgacgaatac aaagtgccat agaagcacca
    17821 atcagtccaa gccccacgat aaaaacatgt tgtgccatct tatttgcccc ctttttagta
    17881 atgtttcaag tagtcacggt actccttcac tgcagctttc aactcaacaa gcgaatcact
    17941 tgggaatttt gacaatagct ctgtcgctac cacagtggct acaacatttt ccgcaacgac
    18001 gctagctgca ggaactgctg tactatccga acgttctaca cttgctttgt aaggttcttt
    18061 tgtatcgata tcaacacttt gaagtggctt gtacaatgta ggaatgggtt tcatgacccc
    18121 gcgaatgaca agtggttcgc cattggtcat tccgccttcg aagccgccta gattattggt
    18181 acgtcggaaa tagcctcttt cttcttccca taggatctca tccatcactt ctgagcctgg
    18241 taatgaacca gaagtgaatc cgaccccaaa ttctacgcct ttaaaggcat tgatactaac
    18301 cacagcttgt gcgattttgg catctagctt cgtgtcccat tgtacgtagc ttcctaatcc
    18361 agctggaacg cctccaacga gcacttctac aattcctccg atcgtatctc catttttctt
    18421 tgtctgatcg atcaaatctt ttattctttg ctcaactgct ggatcaacaa ctcgtacttc
    18481 tgacgcttca gaaatttctt tgatttcttg aatcgagata tttccaggta tttctgcttg
    18541 gattccacca agacaaatca cgtgtcctgc tacgatgatg tcaagttctt ccagaatttt
    18601 cttggcaatt gcgccgattg ctacacgcat ggtcgtctct cttgcagagg accgttcaag
    18661 cacattccga agatctcgat gatggtattt cataccgcct accaaatccg cgtgtcctgg
    18721 acgcggacgt gcaactcgcc gcagtgattg gtctttttca ggcacttctg ctacagacat
    18781 gacagttgtc caatttttcc aatctttatt ttctaccact aaagtgacag gtgaaccaat
    18841 tgttttgccg tgacggatgc cagacgtgat ccgaacacga tctttttcga tcttcatcct
    18901 tcctcctctg ccgtaaccag cttgtctgcg agcgagttcg tgattgattt cttcttctga
    18961 cagaggtaat cctgctggta gtccttcaat aatggctgtt aattctggtc catgggattc
    19021 tcccgcagtc atataacgca tcttacagtc ctcctaagta atctttgaat gtctctgtgg
    19081 gaacaggaac gatttttgct tgcccaatct tttctagcaa gatgatattc aattggcttc
    19141 ctcttgcttt cttatcatgg gttatcgcgt ggaataattc atctggatga cttagatcgt
    19201 acgtaatcgg cagatggaat ttttgaagta tcctttctaa agcagctgtc gatccagttg
    19261 gagacaagcc gattttttct gcatgagaag tgatggctac catcccaata gctactcctt
    19321 ctccatgact gactacccca tatcctgctg tattttcaat tgcatggcca attgtatgac
    19381 caaaatttaa aataagtcga tttccttggt caaagacatc ttcttccact acttttcgtt
    19441 tgatttccaa cgtagcagta atgattggaa cagcatgttc cagaagatct ttttcgtctt
    19501 tcagctcgtt cagctgtttc caaagcgttg catctgctat agctgctgat ttgatgattt
    19561 cagcgattcc ttctcgtaaa cgacgtaccg gcaaagtttt tagaacctca ggatcaatca
    19621 atacaccagc tggctggcta aatgttccga caaggttttt cgctttgttt gtattgactg
    19681 ctgtcttccc gccaatacta gaatctactt gtgcaagtag cgtagtcgga atttgaacga
    19741 aatggatccc acgcatgaac gtcgaggcca cgaatgctgc tagatcaccc accattcctc
    19801 caccaagtgc aagtatccca tcgctgcggg taaaatcatt atctgctaaa aaatcgtata
    19861 actcagctgc ttgttctaaa gatttactgg tctcacccgc cggtacgatt gaaaggactg
    19921 gttcaaaatt agcttgtctc aattggaaca tgattttttc tgcatatagt ggagcaacgt
    19981 ttgtatcagt gatcacagct actttttgag gtctccaaat cgtttgaagc catgatccag
    20041 cctgttctag tgcgcctttt tcaatatgaa tatcgtattt ttggttatca acatgtacag
    20101 taagcattcg tttcacttct ttcttttaaa aagctggaat taatcgagtg tctgaatctt
    20161 ttccattgct tctttcatga tcgcaacttc tttcatcatg cgtgagtaag tcttttcatt
    20221 caacgattgg ggaccgtctg accaagcatt tgcaggatca ggatggatct ctacgatcat
    20281 gccatctgct ccgcttgcca cacccgcacg agccattggt ggtacaagtt cccaaattcc
    20341 tgtaccatga cttggatcaa caatcacagg gaaatgagtt aattttttca tcaatgggac
    20401 agcacttaaa tcaaacgtat tacgtgtcgc tgtttcgtaa gtccgaatcc cacgttcgat
    20461 aaaaatgaca ggagatttgt cttcgactgc aatgtattct gcagcattaa gccattcatt
    20521 gatcgtaccg gaaattccac gtttcaatcc aacaggttta cctgttttac cgacagcaga
    20581 tagcagttta aaattttgca tattacgtgc gccaatctgt aggatatcgc tgtactcagc
    20641 caccatatcg atatgccctt catccatcac ttcagtaatc actttcattc caaattcatc
    20701 agccgcttga cggatgtatt tcaatccttc ttcttctaaa ccttggaaag catatggaga
    20761 agtacgtggt ttgaaggcac cacctcgcag aatttttgcg cccccggctt tggccattcg
    20821 cgcacattct ctgatttggt ctaatccttc gatagaacaa ggaccagcca tcgtcacgaa
    20881 actgccgtca ccaattttta ctccgtctac atccacgact gtattgccag gatggaattc
    20941 tcgagaagtc aatttgtatg tgtttaaaat acgcacagca ttttctactc cttcatagcg
    21001 tgtaaatgct ccttcttgta aacttcgcgt atcacctttt agtccaatca ctactcgttc
    21061 tgttccttga tcgattgaga ctttgaatcc ttctttttcg atatgatcga cgactttttt
    21121 tatttgttct tccgttgctt cttttttcat aattactatc attgtttttc ccgctttctt
    21181 ttgtttactt atctatcttg gctgattttc tctacttttt caaggtggtt ttacattaat
    21241 tcctactaaa tgaccgtgtc ctgttgttct cattttcgct ccttctttcc tacttatgac
    21301 ccagataatc aacaaaaaaa gatgtccctg taggaaattg cctctacagt gaacatcttc
    21361 aacgagcctt aaaaagcgct gcaaaaatat aaaatagcca ctatagatga atttctctac
    21421 gtggctgtaa gtcgtgttta aaaatcttta gccatcatag atacaaacct ggtttgtttg
    21481 tacccacgaa gtcatgaata caaacctatc taaaatagct aaagtaatac gtattcagtt
    21541 ttgtaagttt tctagatttc ataaaaaatc gctccaaact gatgtaagaa ttgtacctag
    21601 tatagacata aaaaaatcga ctgtcaatca tttttatgaa acttttttta attttctgat
    21661 agttaacttt gtttttcaga aaaataacaa ttggttttat tgaagaaaat agtcatgaac
    21721 aaaagtatct gagaactttt ttactctctt gttcatgaca tatagaataa agcagctttg
    21781 tcttgtaaac tccgtttttc aaataataat ctaaagattg ctactgaata ataaagccga
    21841 tttcatggtc ctcttcgctg ctgattcggt aaggtgactc aacatccgct ccccagctat
    21901 tgataccccc gactcctctt acttttgcaa aaatcgtaag aaccgttcgt ctttctggcg
    21961 gtagttcttc tatatgtgtt gcctgttcca gttcttctgc tttgaatggt aaagcggaaa
    22021 aagcaaatgg cgtatcctgc tgttcaaatc gcaacgaaaa atcttcttga tctttatccg
    22081 tattatccaa cgtttgattt cttgttagtt ctacccattt cgtttccata tgcatgccgc
    22141 attcttgggg aacaaggtat ggggaaacgg ggagtcctgc cactttataa ataccttcga
    22201 caccaccagc catccgatcc ggatatgttt cccctgatag gccttcatac tcaaaaaatt
    22261 ttgctttcgt tggcataatg aaccttaaac ccaatgcggg taattccggt aatccttttt
    22321 gtccataata atgcatattg acaagcattt taccctctcc agcgatttgg taggaaattg
    22381 tcacttgggt ttttggtact gtgttgattt ccagtgtaaa aatgatttca accgtttcag
    22441 cgtattcatt acttgaatat tgattattta ctggagcaat cggcaaatcg atctttttat
    22501 tgtccaccag tacttctatt tctttgactt ttggatacaa tccggctcct aaccacatac
    22561 cggatttaaa tccaaatccg ctacctctgt cattatcggt cgttgctcgc caaaaaactg
    22621 gaagtgtttc ccggtatagc cattcttttc cgtttttatt gagcgattcc agtccgcctc
    22681 ttgtataaga aaaaatataa tgcgtattcc ccgatttgac acctaatgta cagtcgccga
    22741 agacgattgc aactttttcc gtatccatat gacttttatt tactgtattt ttcataataa
    22801 tatcttacct cctgcatggc gggtttttct tgtccatctg caaaaacgat cccattccct
    22861 gagaattcat agtcacttgg tttatcgtca aagtcaccgc catagcgcat cacttctctg
    22921 cctgtgattg gatcagtcac ttggatcgcc tgatctttaa aatcccatat atagcctcct
    22981 tggtacattt cataacgatc aagcaacgtc atgtaagaat ccattccacc taacgaattc
    23041 cccatgtcat gcatatattc acataagatg aacggttttt ccgctttatt ttcaaggtag
    23101 tcaatgatct cttctggtgg cgcatacatg cagcttttta catctgaaat cgaatcttcg
    23161 aatgctggat ttggaaaatt cccttcataa tggacaagtc gatcggggtc tacttttttg
    23221 aagtaatcgt tcatttgtcg aatgttttct tccgcataag attcatttcc aagagaccaa
    23281 aaaagaatac ttggatggtt ttttaatgtt tcgaaattcg tttttgctcg atcaagaaca
    23341 gccgcctgcc actgaggtac tgcacctggt acgttgtaag aaggctcaaa tgcgcccatt
    23401 ttttgccatg agccatggga ttcgaggttc gtttccgcca tcacatggat tcctgcttgg
    23461 tcgcataaat aataccaagt gatctggttg gggtaatgag atgtccgtac gccgttgatc
    23521 tttgcatttt ttatgatagc caaatctttt tccatatctt ctatagtgat ggcgcgtcct
    23581 tttttcggat gccactcatg tcgatttacc ccgttgataa tcatccgttt tccattgaag
    23641 tagatgattt tatctttgat ttcgatttta cgaaaaccga aggaatagtc aatatactcc
    23701 gttactcttt tttcttcgtc tatcgtctcc aattcaagct tatataaatt gggctgaaaa
    23761 tgcccccaca gtgccacatt ttcaaatcca tcgatgcgaa tcgtcatttg ctcttttaac
    23821 ggatattgct gttgatagat ttccttccca tcaggatcta gtatcgctac attcagctga
    23881 ctgtttttgg ctacttgctc aaaagacaaa tctacttcaa gtttgccatt ttgacatttg
    23941 tcatctaagc gaggtttgat ccaaagatct tctaaatggt tttgtcctcg agcaactaag
    24001 aagacatctc tgaagatccc aaaaaatcgg aaaaagtcct gatcttccaa gaatgcagct
    24061 gtacttcttt tgtaaacctc cacagctaga atattttctt cttccaaata aggtgttaaa
    24121 tcaaattcag acggagtgaa cgaatcttcg gcatacccga taaaatgacc attcaaccaa
    24181 ataaacattg cttgttctac accagcaaaa tgaatcgaga tttgttttcc ctttagacct
    24241 tcttctaatc gaaaagtcgt caaatacgaa ccaaccggat tgtctttagc agctgaaaag
    24301 atcccttccg cctctttctc agggcttgtc gtgaaagggg gacggcgaaa tacatgtccc
    24361 tcccacggat acatcgtatt gatgtaatgg atcttgtcat aatttgctaa ttcaatatga
    24421 ctaggaacag tgatttcatc aaaagaacag gcatcaaaat cttttttata aaatgttttt
    24481 ggtcgtaccg aaggatttgg cgaataacaa aaacgccatg atccatttaa agactgttta
    24541 agggaggatg tggtttctac ttcttccctt gttgcataac aaacatgatc gctatgtgca
    24601 tttgattggt tcactctaaa aacttgtgga tcatctaacc attttatatt tgctttcata
    24661 cttttttcct tctctctcat aactttttga aatgaagcag ttctgtttgg aaatcacctt
    24721 gaaaatcggg gaagtaaagt ccaactgtca tcaattcggc ccccgaaaat atccgcttcg
    24781 aggtctgttc ctgataaagt gcctcatcat ctaaccccat cagatatact tcatggaacg
    24841 ctggttgagc tgaagctagt atcctcccta aaaacacaat agcttcatct tgtttaggag
    24901 acacaaacat ccatgcggca atattttctt caaaaggact cgccaaacga taataatggc
    24961 catactggat caccggacga atcgtttgat aggatataat ttggtctttt acttgctttt
    25021 cttcttcagg agacagcttt gtcaaatcca gttcataacc aaaaacagcg ctcatagctg
    25081 tttctcctct tgttttcaac gaagtagatc tgtcggtctg atgatttggt acagctgata
    25141 catgagctgt gatggacgaa atcggataag caaggcttgt tccatattgg attttcatac
    25201 gctccactgc atccgtgtta tcacttgtcc atgactgcgg catataataa ataaacccgg
    25261 catcaaaccg tccacctcca ccagaacagc cttcccataa gatttccgga taatcagttg
    25321 tcaaatgttc aagtagttca taaagcccga gcatataacg atgcgctact tctccttgtc
    25381 tctgtgggtc aagggtaatc gaatagacat ctgaaagcga acggttcata tcccatttga
    25441 tgtagtcaag aggaatcgta tctaaaatct ttctcatttg tttctcaatc gtctgccgaa
    25501 catctaacct tgtaaaatcc agtacatgtt gtgatctgga agcagaaggc attctacctg
    25561 gctcctgcat aagaaaatca ggatgtgttc gatacagttc agaatcaacc gagatcattt
    25621 caggttcaaa ccataaacca aatttcaatc ctttgctatg agcataatct gctatttcac
    25681 gtaatccatt tgtcaacttg ccttcatact cgaaccagtc tcctaatgaa gaattgtccg
    25741 catctctttt tccaaaccag ccatcatcta atacgaacat ttcgatgcct agctcctttg
    25801 cttcatcaat gatcgccttg attttttcac tattgaagtc aaagtaagtg gcttcccagt
    25861 tattgaccag aatcggccgt tcagcaaatt gatgacgacc tcttgcgatt ctttcacgta
    25921 gcaactcatg gtggatctga ctcattttgt tcaatccctg acttgagtaa gaaaggatca
    25981 tttcaggtgt ttgaaaagac tggcctgcat tcaattccca cgaaaaacga tggctgttga
    26041 tcccaccgac gactctcaat tgattgatct gatctttttc aagttcgaaa gaatggttgc
    26101 cggaataaac aaattgaagt ccgatcgctt ctccagtatt ttctgtcgta tgatgatgaa
    26161 ctaaagcaat aaagttattc atatggtgac tgctcgttcc tcgacggctt tcaaatactt
    26221 ttgttccaga taaaacagat tgccgtgaaa tctgtctttc attgacatgt gctcctggca
    26281 aagcaatgac ttcatcaaat cgcctagtat gggcaaagtc tagctggaaa ctagccgctt
    26341 tttctagttt gattgtttct ccggtttcat ttcttatctt tacagatcgt gtgatcactg
    26401 ctctatcagt aaatatggta tagaacaaat cgaaataaat cgctgtttct ctatccttaa
    26461 gtgaaatcac caacgtttgt gcctctgact tgtccatcac ataagtttga ggcaagcctt
    26521 cgatctctgg cttcccatct gtaatataat ggctgtcata tctcagatcc agtaagtctg
    26581 atccattctt gcgctgaatg atcgatgcgg gcgttcgaaa gtccatgctg ccatgagaag
    26641 agtattcttg cggtagtgta tctttcgaca gagaccggtc aggatttaag ggaacatttc
    26701 ctgaaaatcc acgatctctt cggggataat ttttattgtc tttgtactgc ttgactggtt
    26761 tgccaaaata aatatgcgcc aatacattgc tttcttctat ctttatcaga taagaaagaa
    26821 atgtatttcg taaatggaag actcggctct tttcatcaaa taagatgaaa tttttatctg
    26881 tttttctatg cgtaatatcc atcatttcac tcccaacaaa acggaaaccg tttacttttt
    26941 tttataattc ctgtatactg tttactaaac aattttgtca ataaaaattt tttatttttt
    27001 tgaggagtgt gtaaaaatgg ttggaattag agatgtcgca cgaaaggcaa atgtctctcc
    27061 tgctacagtt tcccgtgtgt tgaataacga tcagacgatc catgtggctc agactactcg
    27121 gcaacggata gtagaggcag ctgctgcgtt ggattatgac atcagtactc gtaaatatat
    27181 caaaaagaga atgccttcga ttggtgtgat ctctacgatc ggcaaagcga gcgaagaaca
    27241 ggatatctat tacaaacatt tgcgagtagg gatggaagaa gaagctcgac gtctgcattt
    27301 agggatgaat cgaatctata atctatctga taatcctaaa caatgggaag atctagatca
    27361 gttaggtgcg gtgatcgttg ttgggactgt tacgaaagaa tcgattaccg ccttgttaaa
    27421 acaaaacatg caaatagtcg tcgtggataa ccctgacatc cagcaagagg tcgatatggt
    27481 gtatgcagat cttgaacgga tgactaaaac cgtactggaa ttgttcttag aaaatggtca
    27541 taaaagaata gcctatatcg ggggatataa agtcgatatg gatgaattcg gtgggaagca
    27601 gatttctata aatgaaaaga ggatgcgtgc ctatcagcat tttatgatcg atcatcactt
    27661 agaaaaatat atgtcttatc atctaggtgg ctggacagaa aaacaaggtg aggtacttgc
    27721 gactaatcta ttagaaaatg aatcccctct tcctactgcc attcttgtcg ggagcgatcc
    27781 aatggcaatt ggtgtctatc gggcattgaa acaaaaaaat atccaaattg gagaagagat
    27841 tgttgtagca agctttgata atatcgagac aatcgcaaac ctctcccctg gcttgaccag
    27901 tgttcaaatc aatgcgaaat caattgggca agcagctgtt cgaatggcat tggagcgaat
    27961 tgacggtata cgggacgaac caatcatttt gacttatcct gcacgattga ttgttaggga
    28021 aagttttatc ccaagaacaa aaaggtattg aaagagaggt tgtgaaaagc cgcttcgttt
    28081 cttcatactc aaaacgaaac ggctttttca caacctctta cttatactta ttcacaaaat
    28141 ccaatgtctg ctggtaacaa ttttgcgatt cttttagcgt gtaatcaaat tggtattcat
    28201 gggtaatctc tttttttgta tctgtataaa acaaggattg cacgggtacc tttaattctg
    28261 tcaaccgatt ttctaatgct tgaccttgct cctggaatga gtatgcattt ccgtcagtta
    28321 tatacgttgg cggaaattcc ttagaaacat ggtcagccac actagcttct ttcaattgct
    28381 cactcgtttt ccaatctttt gttccaatca acgaccacgc aacggttttg acgaaaaact
    28441 tcataaatcg atcatccgaa tgctgctgtg ctgttagctt caaatcaaca ggtccgcaat
    28501 aagaaatggt tcctttcaga ctatcttttg gaagtagctg tttgattttc atttgtttag
    28561 catacgcatc attcgtttgt atagctgcat attgcaatgc gatttgtgaa cctgcactat
    28621 ctccaccaaa aaataattgt tccatgtcta gcaatttgtc gtctttttct tcagctaata
    28681 aatctttgat cagttcattc acttgaagaa cttgattagg gtattctgaa tcaggcgcta
    28741 attcataatt catcgcaacc actgcgatgt tggcgtctga aactaatttt gtcgcaaatt
    28801 ctttgacacc cgatttatct ccgccgacga atcctccacc atgtacccaa aatagaacag
    28861 gaacaggccc cttgctgttt tttggatagt agatgtcata agtattctct ttatacttgg
    28921 actcatacgt caaatctgtt tttttcgcca cgtttttttc tgcttgatcg aacgtttttt
    28981 tatcagtgat ttgaacagta tttgaaaaca tatggttgat caagaatgct ccaggtctgg
    29041 gggaaagctg gaaggcaaga aagattgcaa acgcgatccc aagaatgatt ccgaagaacc
    29101 atccgataaa tttaagaatt ttctttttca taaggctccc tcagtagtta gtattaagca
    29161 ttctgattat accatagtgt tatttcctat caagctctcc tagatgattt aatgattttc
    29221 cccaaacttc ttctgcaatg cttttgacaa aagaaatttt ttccattgtt cttcaaacaa
    29281 aacattttct tcttctgttg aagcaaatcc acattgagga cttaagcaca aacgatccaa
    29341 aggcagatat cgtgtcgctt cgtagatccg gtcaatcact tgctggtgct cttccatttc
    29401 gccatttttc gttacagtca aaatctaaga caactaattt atcttcagag actttctcta
    29461 aaagaacaaa actcccagca cgttcactat cgtattctaa ataataggca cggacatttt
    29521 cttgagtaaa ataatgggtc tgctacgtcg tcatcccccc tgatgccgcc aaaatcgaat
    29581 aataatttcc gcgtcaaaca tgtatattta gtgttgaatc atttgagaat atgaaaataa
    29641 aaatcttttt gatcaatcag ttaatcaaat tgaaataacg aaaaatctga gacagaatga
    29701 cgttcattct gacccagatt tgattttgca caactcaaca gtccttattg gcaaatcgcc
    29761 aaaataatgt agactaacta aaaagactaa gaaacaataa caaaagttaa cacttcttag
    29821 tctttcttta gcacgtctat ttattaaaga ttttagggcc ttttctcatg tttttacgat
    29881 cattcttagg tgtttccatt tgtttggatc ggcctccacc ttgtttcttt ttgatcaatt
    29941 ctttcatttt ttctttttgg tccattttta ttctccttta acgaatgaag cgactaattc
    30001 gatatgatgg gtttgcggga aaagatctac aggctgaact ttcttcacgg cataacctgc
    30061 ttctgcaaat aacttagcat ctcttgcaaa agttgccgga ttacatgaga tatagacgat
    30121 tcgttctggt gacatttcga ttgctgattc aataaaatga tgatctaatc cttttcgtgg
    30181 agggtcaact acgatcacag atggtttgat ccctttttct tgccattttt tcataacttt
    30241 ttctgctttt ccgacttcat aatgaacatt ttcgatttta ttatttaaag cattgaactg
    30301 tgcatcttca attgcttctg gaataacttc catcccatac accttatcca ctctgtctgc
    30361 taaagacaag ccaatcgtac ctattcctga ataagcgtcg atcacaatgt cttttttatc
    30421 taactcggca aactcgatgg ctgtttggta cagatactct gcttgttgag tatttacttg
    30481 ataaaatgac tttgctgaga ttcgataggt ttttcctaat agctgatctt cgatatagtc
    30541 tctgccatat aaaacttttt ctttgtctcc taaaatcaca tttgtttttt cttcattgat
    30601 attttggatg accgaaacca cttctggcaa ttcttcatga atagcttcag caatttcctt
    30661 ggatttaaag aatttttctt tacgagtaac taaaacgacc atcatttcat gggaataatg
    30721 accacgacgg ataatgatgt gacgaagctg tccttcattt ttttcttcat tatatcctcg
    30781 tacttggaac cgttgcaaga tatctctgac tactttgatt gcttgatcga tgacaggatc
    30841 ttggatataa aaatcttcga tagggactag atcatggcta ttctttttgt aaaaaccggt
    30901 ggctaactgt ccatcgatac ggcgaacagg gatctgtgct ttattacggt agccgaatgg
    30961 ttctgccatt ccgaccgttg gaagtacagg aacttctggc atcttagcaa tcttcgccat
    31021 tacatgctct acttgttttt gtttgaagac aagctgttgt tcatacttca aatgggctaa
    31081 aggtgcgata cctatgcgaa gtaatgcatt gttttctaca ggctgacgat caggtgattg
    31141 tgtaataaat gtttcgactt ttccatatcc aaacttattg cctgctttta ctactagtat
    31201 ttctacttct tctccaggga gtgcattttc gataaataat ggataaccat cgatttttgc
    31261 tacccccatt ccttcataac tcaagtcgac gatttcgact gtatatcgtt cgtttttctt
    31321 tactatttgc tgttctgtca tattttgctc ctttaagcct atttttcaat cctactgaac
    31381 tctactataa atcagctaat tacacaacta aaaccggcac tgaccttact acgttcagca
    31441 ccggcttttg attaattctt ttgattaggt gtttcgtctg ctatttttcc gtcaccgtcg
    31501 atatcttcat ccgttaaacg ttcgacttct tttacgaatt ctttgctgac aacctctaat
    31561 tcttcttcat ctgtacctgt gattgcttca tcaggaatct gatcggtatt ggcaaagaac
    31621 tcaatgtgtt gatgaagatt ttggaaatgc atcggtgctt ccccgccgta ttcaccatct
    31681 aagttgatca ttactggtcg attgatatca tcttctgttt caacgaacaa actgcttgtt
    31741 ttggtataga ttattcttgg atcatcgaca tgtcgaccgc cattcagcat gagtgctgca
    31801 atatgcaaga tctcaaagat atttgctgtc ttcacaatga ttaaagaaaa cttcccatca
    31861 tccaatttgg catctggcgc gatctgttcg aatcctccaa cagagttcgt cagccctagg
    31921 aaaaacatcg aagcatttcc gatatattcc ccttcatcat acttcaagcg cattttgatt
    31981 ggctttactc gagggagcat ctctgctcct ttcgcaagat aagcaagata accaaaaata
    32041 ctctttagtt ctgatggaac ttcataagtc aattctgtca aatagccacc tgcagcgata
    32101 ttgatgaagt aactgttatc agaaaggccg atgtccattt ttacggtttg gtgttttttt
    32161 atcacttctg ctgctgcttt aatattatct ctaggaattt ttaatgctcg agcatagtcg
    32221 ttcgttgtac cagctgggat gatggccatt ttaggacggt gttcaagcgg agcgattcca
    32281 ttaaccactt cgttgattgt gccatcgcca ccagctgcca ctactagatc aaaacctagt
    32341 tctgcgactc tacatgcttc attcttggct gaatctggtt ctggtgtagt tgcataggcg
    32401 ctggcttcaa atcctgcttc ttctaatgcc tgtaggatat ccgccaaatt ttttttcaat
    32461 aattcttttc ccgaggtggg gttataaatt acgcgtgctt ttttcatttt cagcctctat
    32521 cgtttcgcta attcatcttt aagtagctta ttcacgacac caggatttgc ttgtccttta
    32581 gttgctttca tgatttgacc gactaggaag ccaaccgcac gatcttttcc atttttgaaa
    32641 tcatcgacag attgttggtt gttatccaaa atttcattaa tgattggcag tagtttgttc
    32701 ggatcagata attgcaccca accatttttt tcaactactt cttgggcttc tccgccattt
    32761 gtgatcaatt cacggaagac ttttttggcg atttttgagc tgatcgttcc atcggcgatc
    32821 aaacgaatca ttcctgctaa gttctcaggt gtcaattttg tctcagctaa ttccagtttc
    32881 tcactgttca agtaagcaga aacttcaccc atcaaccagt tagatgcttg cttggcatct
    32941 gcaccatttt ccaaaacagc ttcaaaaaaa tcagacattt cttttgtaag tgtcaatacc
    33001 attgcgtcat actctggaag tccaaactca cggatatagc gttcgcgacg agaagctggc
    33061 atctcaggca aactagcttt tactttttga atccattcgt catcgatcac gaatttcggg
    33121 atatcaggct ctgggaagta acgataatca ctagaccctt ctttgacacg catcaaaact
    33181 gttttattcg ttgtttcgtc aaaacggcgt gtttcttgtt ggatttctcc tcctgaaaga
    33241 agaacttttg cttgacgttt ttcttcaaaa gctaaacctt ttttcacgaa agataaagag
    33301 ttcaagtttt tcaattctgc tttcgtaccg aactcttctt gtccataagg gcgaagagaa
    33361 atattggcat cacagcgcat agagccttct tccattttca catcgctgac ttctgtaaat
    33421 tggataattg atcgcaatgc ttctagataa gcgtatgctt cttctggtga acgcatatca
    33481 gcttcagaaa cgatttcgat caaaggcgtg ccttgtcggt tcaaatcaac gtaagaataa
    33541 ccgccaatac catgcatgtt ttttccagca tcttcttcta aatggacacg ttcgatccgg
    33601 atttttttct ttttaccttc tacttcgatt tcgatccatc cgtcatgacc gattggttga
    33661 tcaaattggg aaatttgata tgcttttgga ttatctgggt aaaagtaatt tttccggtca
    33721 aaatgagtat ctttagagat ttcacagttt aatgccaaag ctgctttcat cccgaattcc
    33781 agcgcagctt tattcattac tggcagcacg cctggataac cccagtcgat cacgtttgta
    33841 ttgctgtttg gttctgcacc aaaatgagct ggcgctggag agaaaatttt tgagttggtt
    33901 tttaactcga cgtggacttc tagtccaatg actgtttcaa aattcattcg tctttccctc
    33961 ctaaaatagc tggttgttgt ttatggaatt ctgttgcttg ttcaaacgca tatgccgctt
    34021 gatacatcgt tttttcatca aaataattac cgatgatctg caatccaact ggtaatcctt
    34081 ctgataaacc tgccggtaca gacatacctg gaagtccagc caagttgaca gggatcgtta
    34141 agatgtcact catgtacatt gtgattggat cgttaatgtt ctcaccgatt ccgaatgcaa
    34201 ctgttggtga agatggcccg atgatcaagt cataatctgc aaagactttt tcaaaatctc
    34261 gtttgatcaa tgtacggact tgtcctgctt ttttgaagta agcgtcataa taacccgcgc
    34321 ttaaggagaa tgtacctaac atgatacggc gttttacttc ttcaccgaat ccttctgaac
    34381 gtgagttgac atatacatca tctaatgatt tcacattttc agagcgataa ccgtagcgga
    34441 taccatcgaa tcgttgcaaa ttggaacttg cttctgatga ggcaatgata taataaacag
    34501 ccacaccgta ttttgaatga ggcaagctga cttcttccac aacagcacct aatgaacgga
    34561 atgtttctgc tgctttcaat acagcttctt ttacgccagg ggccacacct tcgcctaagt
    34621 attctttagg taaagcaatc ttcattcctt tgatgtcgcc tgtcagtccc tctgcaaaat
    34681 caggaactga aacgcctgaa gaagtaccgt ctttttcgtc ataaccgctg atagctgtca
    34741 atgctaatgc attatctttc accgtacggg tcattggtcc gatttggtca agagatgaag
    34801 caaaggcaat caaaccaaaa cgtgagacac ggccataagt aggtttcatc cctacgatgc
    34861 cgttaaaagc tgctggttgg cggatacttc ccccagtatc actacctaaa gaaactggaa
    34921 tttgtccagc tgcaactgct gctgcagaac caccagaaga gccaccaggt acttttgttg
    34981 ggtcccaagc attttttgtt ttcttgaaat aagaagtttc tgtacttcca cccatagcaa
    35041 attcatccat gtttagctta ccaacaggga tcatatctgc ttgatagatt ttatccatga
    35101 ccgttgcatc ataaattggt tcaaaattgt aaaggatctt agatgcagcg gttgtcaaga
    35161 tatcttttgt tacgatatta tccttgatac caattggaat accagcaagg acgttgcttt
    35221 catcaatccc tttttcatct aaagcttttg cctgttccaa tgctttttct tcgttcaatg
    35281 taatgaatgc ctcgacctca ggctctgttt ctttgattcg ttcaaatgtt gtttttgtca
    35341 aatcttgtac agtgatttct ttcgatacaa gcatatgatg cagttcttct agagaacgat
    35401 tgtgtaattc tgtcattatg cgccagcctc cccattatcg atgattgcgg gtactttgat
    35461 aaagccgtct tctgtttccg gcacattttt caataattca tcgcggttcc atccagcttg
    35521 cgcccgatct tgtcgcatca cgtttactgt ttcaaccaca ttcgacgtaa atggaacacc
    35581 ttccgtatct acttcttcca gcatttcaac catatcgatg attttaccta attggtcggt
    35641 aaaacttgca agttcttcat ctgcgaacgc aagttttgct aatttggcaa cgtgtttcac
    35701 ttgttcttca ctaatcgcca tactacccct tctttctaaa cgagttaaaa actttgctct
    35761 ctattttaac acaagagtgc cgtgtctgaa attgtttatt gaaatttgtc tgaaaaaact
    35821 catttttcat tcttctactg agcgactttt agccaatgca tctgccattt gatcttctgt
    35881 ccaaacttcg attcctaatt cttgtgcttt ggtcagcttg ctgccagcat cttctcctgc
    35941 taccacgata tccgtttttt tcgatacgct accagtcact ttcccgccaa gattttcgat
    36001 cgtctctttt gcttcttcac gagtaaatcg tgttagttta ccagtaagga ctactgtttt
    36061 ttctttaaaa ggagactcga cttcttgcaa ttgggcattt cgaagccctt tgtattcgaa
    36121 attgacgcct gcttgtttta attcattcat caattcatgg acttcttcgt tactgaaata
    36181 agtgactaca ctgtcggcaa tgatatcacc gatcgtatca agcgcaataa tcgactcata
    36241 gtcactttta ctcaatgttt ctagatcacc gaaatgttct gctaaaattt ttgctgcttt
    36301 cgctccgaca tgtcggattc ctaacccgaa aatcaaccgc tcgaccgaat tatctttgct
    36361 gttgtcgatt gctgtcaaaa tattatttgc tgatttttct tttattttgt ccaacgtcaa
    36421 taactcttct tctgtcaatt tgtaaagatc agcaacatct gctactagtt ttttatcata
    36481 catctgttct agtacgcgcg gccccaaccc atcgatattc atcgcatttc ttgaaacaaa
    36541 gtggttcaaa ccttctttca tttgagcagg gcatttagga ttgatgcacc gtaaggctac
    36601 ttcttcatcc aaatggacaa gttcacttcc acaaactgga caatgtgtag gcaattggta
    36661 ttcttcgctg tctttaggtc gtttatctaa cacgacttgc gaaacttctg ggatgatgtc
    36721 tcccgcttta taaatcagaa ctgtatcttt caagcggata tccttcaact taatataatc
    36781 accgttatgc aggctggcac ggcttactgt cgttcctgct actcggactg gtgtcatgat
    36841 agctgttggc gttactaccc ctgttcgacc gactgtccat tcgatgtttt cgattagcgt
    36901 ttcaacttct tctggcggaa acttgtaagc tgctgcccat ctcggtgctt tgatagtaaa
    36961 accaagttga tcttgtagcg agaattcatt cactttgatg acgattccat cgatttcata
    37021 gggcaaatcc acacgtttgt catgatattc ttcaatgtat gaccacactt catcaatcga
    37081 atgacataga cgtttttccc gattcgtgtg gaatccaatt ttttctagct cttctagcgc
    37141 atcatactgg gtttttgctt gcataggacc gaaatcagct actgtgtata aaaaagtgtc
    37201 cagatttctt ttcgctgtga ttttagaatc taactgacgc aaagaaccag ctgcggcgtt
    37261 ccgaggattc gcaaagatat cttttccctc tgcttctctg tcttggttca attggacaaa
    37321 tgaccgtttt ggcatgaaac attcgcctct tacttcgatg ttcattggtt cttttaattt
    37381 gattgggacc gaccggacag tttttaggtt ttcagtgatg ttttctccta cagttccatc
    37441 cccgcgagtc gcacctctca cgaaatttcc atcttcataa cgcaaagaaa cggaaagtcc
    37501 atctattttt agttcacagc aatagtcaac tacacgtccc actgcttttt gtacacgttg
    37561 atcaaaagct atcagctctt ctttgctgaa aacgtcgttc aaactgtaaa gcggaatatc
    37621 atgagtgact ttttcaaaac cttctaatac ttttccgcct actcgttgag tgggagaatc
    37681 agacgtgatc aaatcaggat attgttcctc gattgctact aattctgcgt attctttatc
    37741 ataaacatag tcttccactg ttggtttatc ttctacgtag tattcgcgag accattggtt
    37801 cagtcttgta cgaagttctt ctgctcgttt agcagcttcg tcaatcgtca tttcatgttc
    37861 catcatttac cctccactac taagcttttt caattggcgc aaaggcagcc aaaagacgtt
    37921 ttactccttg acttgggaaa gccacatcta gttctagatc cttcgacgtc ccgcttactc
    37981 ggacgactgt tcctagaccc cattttttgt gtttgacttt atctccaggt ttccagctgt
    38041 tattttcccc tccagtagca cttttagagg gaacaaccgc tgtctctggc tgtttatagc
    38101 tatatgctgt ttttttagcg aatacaggcg tttttttagg cgtaggacgc atgccttcga
    38161 tttccagtaa ctcctgatcg atttcttcta caaaacgaga aggtcggtta tattgcgtcc
    38221 gtccatataa tgttctggag aaagcatttg ttaaatacaa cgcttcttct gctcgcgtga
    38281 tccccacata tgcgagacgg cgttcttcct ccagttcgct ttcttccatc aatgctcgag
    38341 ataaaggaaa aaccccctct tctagaccaa tcaggaatac aactggaaat tctaatcctt
    38401 tggctgcatg caaagtcatc aatgtcactt gggaagcatc ttcttctaaa ttgtcaatgt
    38461 cggaaacaag tgccagatca tttaagaaga ccgttagttt ttcttctggt gcatcagcgt
    38521 cttcttcatt ttgctgttcg aattgtttgt cgaattcttg tgtgaccgtt aaaaattcct
    38581 ccaagttttc gatgcgtgct tgtgcttcta atgtattttg gatttttaag tcttctagat
    38641 atccgctacg atccaaaacc tctttcgtca attctgtcac tgtcaagtat gggatcatct
    38701 gagtgacttc ttggatcatc tctccaaatg cacctaattg ctgtcctgct tttccaccga
    38761 tatttgccag atccacattt tgcgctgctt caagcagtgg ccattcatga agagaagcaa
    38821 aactgcgtag tttttcgata gatcctggtc cgatgcctcg tttgggcgaa ttaacgatac
    38881 gctcaaaact gattgaatct tgaggattta ccagcacatt caaatacgct aaaatatctt
    38941 tgatttcttt acgatcgtag aacttgtgcc ctccaaccat tttataagga atatttgctt
    39001 tcaacagagt ttcttccatt acacgagatt gtgcgttcgt ccggtacaag atggcaaagt
    39061 cgccgtaatt ccgatgattg cttcgaatct cttcttgcat ccggtctacg ataaaacggg
    39121 tttcgtctcg ttcattatct gcacggtagt aagtgatctt gtttccttct ttgttttctg
    39181 tccacaagtt ttttggtttt cgattgctat tgttttcaat gacttggttt gctgcgctaa
    39241 ggatgttttt cgtggagcga tagttttgtt ctagcaaaat tactgcagca tctggataat
    39301 ctttttcaaa atccaagatg ttctgcatat ctgcaccgcg ccagccgtaa atactttgat
    39361 ctgcatctcc cacgacgcac aggttcctaa atctacctgc caataaattg actagcgtat
    39421 actgtgcgtg atttgtatct tggtactcat ctacatgaat ataatggaat ttgttttgat
    39481 aatacgtgag tgaatcagga tgttcttcaa ataatcgaat cgtattcatg atcaggtcgt
    39541 caaagtccat acattgatta tattgaagct ctttttggta agcagcataa cattttgccg
    39601 caatctcttc gtaataactg ccttgcattt ttgcaaaatc ctgaggggtc tgcaggctgt
    39661 tttttgcttg agagattgtt cctaatatac ttcgaggatc atattttttc ggatcaatgt
    39721 tcagttcttt tagtatccgt ttcatcaagg tcagctgttc agaagaatcg atgattgtaa
    39781 agttccggtt atacccgata aaatcaacat ctcttcttaa aatacgcaca cacatcgagt
    39841 gaaaagtcga tacccagaca tcttcgcctc cagatgcgag aatcgcattg acacgttctt
    39901 tcatttcttt tgcagcctta ttggtaaaag taatcgctag gatgttccaa ggattgactt
    39961 ctttttcctc aatcaaatag gcaatgcggt gtgtcagcac tctcgtcttt ccacttcctg
    40021 cacctgccat tagcagtaat ggtccgtcag tgtgaagaac agcttctttc tgacgggggt
    40081 tcatcccgtt taataattca gctttggatg acattaacaa catcctttct tttatcaatc
    40141 ttgttaatta tagcattttc ttggctttca ttaaactagc tagttcaaaa tcatttgttt
    40201 ctttttatgg atttttcctt gtcaattcgt tgatttcgcg ctaaattaaa agaaagcgag
    40261 gcgtttgaat ggaaaaaaaa ggaaaaatca agaaattaga taagactcag atatatttta
    40321 ttctaaaagg aatatttgtt ggactgctga caggaatcgt cgttagcctg tttcgattat
    40381 ctatcgaaaa gctaagcgat ttagttcgaa gtatctatga aatgagtcgg gaacagccga
    40441 tttacttaat tggcctagct atttgctgca tccttgcagc atttttcgtg ggttatctgg
    40501 tcaaaaatga accggatatc aagggaagcg ggattccaca agtagtggga cagcttcgcg
    40561 gggaattatc aatgaactgg ttctctgttt tatggaaaaa gttcattggc ggtgtacttt
    40621 cagttggtgc gggccttttt ctaggtagag aaggaccttc tatacagctt ggcgcatcca
    40681 ttggacaagg agctggacaa ttgtttcgga gtccatcttc agaagaaaag attttaattt
    40741 ctagcggtgc aagtagcgga cttgcagcag cattcaatgc tcctatcgca ggattactct
    40801 ttgttttaga agaaatccat cactcctttt ctccacttgt ttggctgaca tcatttgctt
    40861 cagcaatcac tgctaacttc gtctctcttt actttttcgg cttgagacct gttttatatc
    40921 taggtgacct tccttctttg ccgttgaaat attatggttc cttagtgatc ttaggagtac
    40981 ttctaggcat cttaggtttt atttatcaga atgtcttgct ggctttgcca aaattttaca
    41041 accgattgcc tttaccagct tattttcacg ggttcgttcc tttcttgctg attttgccaa
    41101 ttggttattt atggcctagt actctaggcg gaggcaatca aatcgtttta gcttttggac
    41161 atgcttcctt gccaatcatt atgattatcg gtttgtttgt tcttcgattc gttttttcca
    41221 tgatttctta cggtgggggt cttccagggg ggattttcct tccgatattg acattggggg
    41281 ctttaatcgg tagcttttat ggaaacatta tggttgattt cgggatggat ccaatctaca
    41341 tcaaggattt tgtcgttatt gccatggctg gttatttcac agcgattgga aaagcaccgc
    41401 tgacagcaat tattttagtg acagaaatgg ttggctctct taatcatctg atgcctttag
    41461 gattggctgc tcttgttgct tatatcgtca atgaccttct cggcggtaat ccaatttacg
    41521 aatcattatt ggaacgtcta cttggaaata ctcagaccgc ctcactaaaa ggtgcaagga
    41581 caacttttga ttttccagta acggcagaga gttctttaga cggattaatg gtacgagact
    41641 tcaattggcc taaaaatatg ctgctaatct ctatacgcag aggaaatcag gaaatcttga
    41701 ctcacggtga tacgatcatg aatgtaggag atattttggt tatcttgact gatgaagcaa
    41761 acttgccaag aatcaagcag gaaatcgagg ctaaatcttc tatcacaaat ttgaagcaat
    41821 cattttaacg aacatggaaa aactatttta caacaactat tcatctacac tacttgattc
    41881 tatatgattc tataaaaaaa gaagaggctg ggaaaaccca gcctcttctt ttttatttct
    41941 atgcttctgc tactgcagga gcttttctca ataaaccata aatcacacca gatacgactg
    42001 ctccgatagc aataaacacc aaatacatga ccgggtgact cagtaagaat acaacgaaaa
    42061 tcccgccgtg aggagccagt aatttgattc cgaaagctcc gactaatccg ccagttaaag
    42121 cagaaccaat aataaaactt ggaatggcac gaatcgggtc agctgctgca aatggaatcg
    42181 atccttccgt gacaaacgat agtcccatca cgatgttcgt caaaccagca tcttgatctg
    42241 ttttgctgaa tttattctta aacaaacgtg tagcaacgaa aattgccaat ggaggtacca
    42301 ttcctccagc cataacagaa gccatcacga cacttccgcc agttgcaaca gtcgtagcta
    42361 aagttgctgt accaaacaca taagcggctt tgttgattgg accgcctaaa tctgccgcca
    42421 tcattccagc taataatgca ccaagtaaag cagcattcga accactcata cttgtcaaga
    42481 aaccgttcaa tgcatcattg attgctttca tagggacatt gaccagcaac atcaagaaac
    42541 cagtaatcag taagccaaac actggataaa agaggatcgt tttaattcca tccaatgatt
    42601 ttggtaagtt cttgaataat ttacgtaaaa atacgatgac ataaccagct aagaatccac
    42661 caatcagtgc acctaagaat cctgcaccgc ctgtattagc taatgcacca gcagcaaatc
    42721 caacgatcaa accagggcgg tcaccgatac ttgaagcaat aaatcctgct aataccggca
    42781 gcatgaaacc aaatgcagct tgtccgattt ggttgaacca gctagcagct tgattgtaat
    42841 tccctagatt tgataattga tcatgtggca cgccaataaa ttggtcgatc atgaaagaaa
    42901 gcgcaatagc gattcctcca ccaataacga aaggtaacat gtgtgatacg ccgttcatca
    42961 aatctttgta aattctttgt ccgatagtac catccgcatt tccttcttct gaactgcttt
    43021 cttttccatc accatggaat actggtgcag taccactaag ggctagattg atcaactctt
    43081 ctggtttacg gataccatca ctgacaggac gattgatcag ttcttttccg tcaaagcgat
    43141 tcatctctac ttttttgtct gctgcaacga ttacaccttc tgcacgggcg atgtcttcag
    43201 cagtcaatcg gtttttgatt ccttctgaac cgttcgtttc gactttgatc tccacaccca
    43261 tttctttcgc ttttttcttc aaagcatctt ctgccatgta agtgtgagcg atccctgttg
    43321 gacaagctgt aacggcaaca acgaattttc tttctggatg agcagaagct tctgcagctt
    43381 gtgtttcttg ttctgctttt tcttctgctt ctttttgctc ttcagctgtt tggaacaagt
    43441 cttggacttc ttctggtgta tttgcttctt ttaattttcc tacgaaatct gcatcaatca
    43501 gcaatcgaga tagtgctgct aatgcttgca aatgtgtgtc gtttgcgcct tctggagctg
    43561 cgatcatgaa aaacagataa gttggctgtc cgtctaaggc ttcataatct actccttttt
    43621 ggctttttgc gaataaaact gttgcttctt taactgcact gtttttcgcg tgaggcatgg
    43681 cgattccatc accaagacct gtagaagttt gtgcttcgcg ggctagaatt ccttctttat
    43741 atgtttcgat atcggaaatc cggccggcat caaacatttt ttggaccatt tcatcgattg
    43801 cgcctttttt atcagtcgct tgcaaatcca tgatcatcgc ttctttgacc attaaatcct
    43861 tgatattcat gattatcctc cttgaaacta atcaattttt gtgatttcaa cttctggcag
    43921 caactcatca atcaattcgc gagctgccaa gtcatcagaa aaagctgttg cgcttccgca
    43981 agcaactccc catttaaagg cttcgattgg atctttcgta cggctgtaag ccccaataaa
    44041 gccagcaatc attgaatccc ctgcacctac tgaatttttc aatgggcgaa ctaatacatt
    44101 cgttttataa gttccttgtt ccgtgaagag caaagcaccg tctccagcca ttgagatcag
    44161 cacattctgt gccccttcag ctagtaattt tttcccatac ggaatgattt cttctacaga
    44221 ttggaaggtt gtgttgtaaa gctcagctaa ttcgtgattg tttggtttga tcaacaacgg
    44281 tttcttaaca agtgcatttt ttaggtcatc acctgttgta tcaatcacga aatctgcacc
    44341 tttttctcga atgatatcaa tcagttcttg ataaaatcct tttcgcaggc tagctggcgc
    44401 gcttcctgac aatacgacga tatctccttg tcctactgtt cctaatgctt gcttcaactc
    44461 atcgatttct tcatctgaaa tagatgggcc taagccattg atctcagttt cagtgtctga
    44521 ttttagtttg atattgattc ttgtatcttc ttttacggtt gtgaacaaag actggatttg
    44581 ttctttttca agccacttcg aaataaattc gccagtgaac cctcctaaaa accctagtgc
    44641 tgtagattga gaatcgatcc gtttcaaaat acgggaaaca ttgatccctt ttcctccagg
    44701 taacttgaag tcttccttca ttcggttcaa atcgcctagt tttaacccat ctacgcgtac
    44761 gatataatca attgatggat taagtgttac tgtgtaaatc attgtattgc ctccttaatt
    44821 gttgtctttt cacggaattc attaacgaat tctaatggac aacgctcggt tatgatcgtg
    44881 gctgcttcca aaggtgcaac agaggtgaag gacaactggt tgaatttggt atgatcaatc
    44941 aatacaaatg tctcttcgga atttttcatg gctaaacgtt taacagctgc ctcttctgga
    45001 tccggagtcg tgaatccttg ttcaagatgg gcaccgttca ttcccataaa tgctttatta
    45061 aaatgaaaat tttttagttg ttctatcgca ttagatccta aaacagcatt agttgccagc
    45121 tttatcgttc cacctaaaat aatggtagca agctccaaat caactaaatt tgccgcatgt
    45181 tgaacggaat ttgtcactac tgtgatctgt tttccttgca gaaaaggaat catttctaac
    45241 gtagtagagc cagcgtctaa ataaatgaca tcttcgtcat taatggtttc agctgccgat
    45301 ttggcgattt gctgtttttc gtgaacgttt ttgagtgatt tttcactcat attctgctca
    45361 aaattcaata attgctcttt cttagctccg ccatgaactc tttctagaaa cccactattc
    45421 tccagctctt gtaaatcacg tcttatcgtt gattcagaag cattcaaaag gtttacaaga
    45481 tcttgtgatt tcacgatttt ctgctgttca agcaagtcta ggattttttg tctgcgttcc
    45541 tctgtaagca ttttcacgat cacctcaata caaataatag catatttttt ttctactagc
    45601 aatataaatc agtcaaaaac gttcataaaa attataaatc agtcaaaaaa aaataaaaaa
    45661 ccagtcagaa agactggttt tttatttttt tgtctatcat caagccgttt tatcgttttt
    45721 tattttcgct tcttgttttt ttcatataaa cagaagcagg ttttctttcc ttatgaagaa
    45781 gataataata aagtcccatc ccgccaaaca tgaccaaaaa accgatgact gatcgaatag
    45841 gaaaattagg aacaaaaaag agacagacgg ataataatga tgccggaacc attattcgat
    45901 aatagagttg tttatccatt ttcttcacct catatttttt gtaaagtaat caattgttct
    45961 aattctcggt aatctaaaaa ttgcacacgc agatgctgct cacctttctt gacatcgaat
    46021 tggatacttc gccgcttttt ctttattcgt ttgaaggtaa catcagagag tctatgtttg
    46081 atttccttta acggtaattc tgacagggat tctgaaggaa catagattct taaggataaa
    46141 agatactctt gatatttttc atctgttacc tctacttcta gatattccct aatataataa
    46201 caataagctt cgattccatt atccaaagca acagtcggat cgataacttt atagcacatc
    46261 atgcgtgatc gattctcgtc aacccaagga aaatacgcct gagcatttag tgttccgcga
    46321 acacgccttt ctttataatc tccttgttgg atatcataac tttttgttac aaaaaatgaa
    46381 gcattctttt taggaacagg ttgcatttgt gcattctgat actggacaga ttggatcgta
    46441 tggtcagtgt aaatgtattc aacgaatggc ggtttcaata aaccgacacc attgtcccaa
    46501 gcaagatgat caaacccaag ctggtcccta aaataggatt gaaacatttg attgtttggc
    46561 ttttgccagt taaaggggtc tcgataaagc tggatacata tgacttcgtt ttctaattcc
    46621 ttcacttttt ctacttgttg gatgtcgaga aaaatcggtt tagggatgat ttgatgataa
    46681 tagcgactat aatacatttc ccaacacgaa gtgaaacata aaccttggta aaaaagatcg
    46741 tatcctggaa attggttgca atcgactact acttccccgt tttgttgttt cattttaggt
    46801 aaatgttcga tttcatcctt tttctcaaac aatagccgtt gagagatctg ttttgtattg
    46861 tgatagataa actcgctata agctcgcata taggcgaaca ccccatgagc aatcattttt
    46921 ttctgaatga agtctcgaat caaaaattgc ttttctttga aaacagagat agacaataca
    46981 ttttttatca ataattgatt atcttccaag cgaaaactac attcgttctc gtcatttcct
    47041 acaactaacc aactttcgtc tcttaccgat gctagcatta ttttttcttg aagctttcga
    47101 agatcatacg gatccgcttg ttgtgtgaaa gagcggataa atgttggctg gatcaacaga
    47161 cgtgtcaaaa tagcccaatc gcttatttcc tgtttttttt cttcttcatt taagtctgtg
    47221 agctggatag tcatcaataa tttatcttcg ccaatcattt tctcacctct ttcaaatctt
    47281 tttataattt aattataacc aatgataaag aaaaactaaa actttatgtc ttagaaacca
    47341 atttggacga ttacatgata gactaagtaa aaagaaacaa agaggtgttt acaatggggt
    47401 tgaaatttga acgtacagat aatttagata aacttttcga atcatttgct gtcgatccgg
    47461 aaaaaacagt tgcaccaaaa gaagagatcg atcgatttct aaaaccacag ccacaaaatg
    47521 aaaagaaaaa agacaagaaa caaaagtaag ttaagctcaa aaaaagactt accagccaaa
    47581 cttttatgaa agagtttgac tggtaagtct tttttattct ttttatgatt caattgcttg
    47641 ttcgattgct gcccacgctt cttcttttcc ctgttttgta acggaagaaa agagaataaa
    47701 cgtgtcattg cgatcaaaat ccaacgcttt tttgatttcg ctctcatgct tgttccattt
    47761 accacgaggg atcttatctg ccttagtggc aacaacaatt acagggatat cataatactt
    47821 caaaaattga tacatctgga tatcttcttg agaaggctta tgacgcaaat ctactaaaga
    47881 aacgaccgca cgtagctggt ctctagtcgt taaatatgtc tcgatcatct ttccccattt
    47941 tgctcgctca gttttggata cttttgcata cccatatccc ggaacatcga caaaatgtaa
    48001 tgcgttctcg atcaaataaa aattcaatgt ctgggtcttt cctggcttac cggaagtacg
    48061 ggctaaattc tttcgatcaa tcaatgtgtt gataaaagaa gatttcccta catttgatcg
    48121 tcctgccaga gctatttctg gcaattgtgt atctggatat tgttttggtg ccactgcact
    48181 tatgataatt tcagcttgat ggactttcat ggtctctctc cttaacaagg tgatgaataa
    48241 agcaagcggc tgtcgcaaaa gccaaaatga cttttacggc agcctctttt catcaacccg
    48301 cttttttctc tgaatcttta tagattactg ttggctcacc agacaattca gctgcttctt
    48361 ttgtgataat cactttttcg atgttttcat ttgaagggat atcaaacatc acatccatca
    48421 tgatttcttc aatgatggaa cgaagtcctc gagcaccggt attgcgttcg attgcttttt
    48481 tcgcaatggc tctcaacgct tcaggttcga attcaagttc ggtattatct agagaaagaa
    48541 gtttttgata ttgtttgact agcgcatttt tcggctcagt caagatccgt accagatcat
    48601 ctgttgtcaa tttctctaaa gctgccataa caggtaatcg tccgataaac tcagggatca
    48661 atccgaattt tagtaaatct tcaggaatga tatgctgcat gacactttcg tcttcagaaa
    48721 gtttttggtt ttgtgtacca aatccaatcg tcttttcgcc catacgattc ttcacaatcg
    48781 tttcgatgcc atcaaatgca ccacctacga tgaacaatat gttcgtcgta tcaatctgaa
    48841 taaattcttg atgtggatgt ttacgtccgc cctgaggtgg cacacttgca acagttcctt
    48901 ctagaatttt cagcaaggct tgttggacac cttcgccgga aacgtcacga gtaatagata
    48961 cattttcgct tttacgcgca attttatcga tctcatcaat atagatgatc cctttttctg
    49021 caagttccac attgtaatca gcagattgaa gcaatttaag caaaatgttt tctacatctt
    49081 cccccacata accagcttct gtcaaacttg tcgcatctgc gattgcaaaa ggtacattca
    49141 atgtttttgc taatgtttgt gctaaaaatg ttttaccaga ccctgtaggt ccgatcaagc
    49201 agatattgct tttttgcagt tcaacatcat ctgtgttttc ttgtgcttgc tgattgacac
    49261 gtttatagtg attgtatacg gcaacagcta acgagcgttt tgcgcgatct tgtccaatca
    49321 cgtagttatt caatacttca agaatttcta gtggtttagg gacttcagtg aattcgcgta
    49381 cagcttcttc ataaaattct tcatcaatga tttctttaca taggtcgata cattcattac
    49441 agatgtagac ccctggacca gctacgatct ttttcacttc ttcttgggtt ttcccgcaaa
    49501 aagaacagcg tacggtctca ttgctacttg gattgtcata catggtcttc accccttaac
    49561 ttgcaaaaag tatgcaagaa actatcaaag actttacagt cttttactat cataacatag
    49621 tttagtaaaa agaaaaagtg tttaacaaag gatcaactga cctatacctt tgcatttttt
    49681 actcttaaca aaaaataagg aactgtgaca aaatcgtgtc acagtccctt atttcgaata
    49741 aacaattttc tagaagtagc ttcttcagaa atgatttatt acttgatttc tattctttcg
    49801 cttctgtcgc tgtacctgtg atcaattcga ttgctttttt cattgtcaca tcatgttcca
    49861 gcatatcttc tgtcaacacg cgtttgattt gttcgattgg catgttgtat gtttctgata
    49921 attcttggat ttctgcattg atttcttctt ctgttgcttg aagattttca gcagctgcaa
    49981 ttgcttcgat cacaagattt gttcttgtgc gcatttctgc ttcaccttcg aattgtttat
    50041 gcaagtcttc ttctgtagaa ccagttaatt gatagtacat ttcaggagag ataccttgac
    50101 gttgcatgtt gttcaagaat tcgtccattg aacgatgtac ttcatcatga accatcacgt
    50161 gtggcaattc aacgatttct gcgttttcaa cagccatacg gatcgctgct tcatcttttg
    50221 catcgtccgc tgctttttct tttgtttcta ctaattcttt gcgatatttt tctttcaatt
    50281 cgtctaatgt tgatacttcg tcatcaacat cttttgcgaa ttcgtcatct aattcaggca
    50341 cttcttttgc ttttacttca tggattgtca ctttgaagac tgcttctttt ccagcaagat
    50401 cttctgcttg ataatcttct gggaaagtta catttacatc tagactttca cctgatttat
    50461 gaccaactaa ttgatcttca aaacctggaa tgaatgagcc tgaacctaat tcaagtgagt
    50521 aattttcgcc ttttccgcca tcaaacgctt cgccatctac aaagccttcg aagtcgatta
    50581 ctactgtatc gccattttca gctgcagcat cttctttgat aactaattca gcttgtgctt
    50641 cttgttcacg tttgatacgt gcatccacgt cagcatctgt tacttcacgg tcttgttttt
    50701 caacaactag atctttatat tgtcctaatt ttacttctgg tttcactgtt acttcagcag
    50761 tgattaccca gtcttgacct ttttccatgc tttcaacatc gatttttggt tgagaaacag
    50821 gatcgatgcc agcttctttg acagcagctt cataagcttc tggtaataca gcattcaatg
    50881 catcttcata taaagcttct tcaccgtaca tacggttgaa tacttgacga gaaactttcc
    50941 ctttacggaa tccaggtacg ttcaagcttt ttttcacttt attaaacgca gttgttaatc
    51001 ctttttggat ttcgtcttgc gcaatcgtaa attttaaaac accatcattc gtgccttttt
    51061 tttcaaatgt tgcagtcatt taattccctc cgagtttaga aattcatttt atacatcaaa
    51121 aacgtttcat gcataccttt aaatagtaca ctaacccttt caaattgtaa acatattgaa
    51181 tggatagaat ccttgttttt cataacattt ttatcttttt cactgataaa atctaatgga
    51241 agaagatcat ttgctgcaaa attttttctc ttttttttaa aaaatcttgt ttttcatctt
    51301 ctgtaccttc atacatcgtt tttcctgtac acgcctctac agtcagttga aaccagcgct
    51361 tcgggtcttc gatgtatgta tcatagacag gataaacaat tgcagattgc aacgtgaatt
    51421 cttcttttaa cacggaacta agcagcgcat cttcattccc gtaacgatct gaaaatacac
    51481 ataattgctg gtaacttgcc tgctgctgcg gctttggcag atcttttggt atgactttga
    51541 ctaacttttc ttcaatcgtc aaatagaaaa caggatggca ttcgttcaat tgcctaagtg
    51601 tctccagtaa ttttgccctt gctaaaatat ggacctctgg cgatacaata agtttactcg
    51661 ctgtttggat aaactcatga taaggaagct gttcgatctc atcaatagac gcaagctgac
    51721 gtgcaggttc caacgaagga agttcctccg atttattgtg gagtgccttg atcttctgcc
    51781 tctccatttg tccggataat tcttctgcaa attggatttt ttcaatgacc cgctgctcga
    51841 aagcttcata aaaatctgta cttgctatca attttctagc atataaaaaa tcttgtgtac
    51901 tgattaacaa gtcaaaataa aattcagcaa actcctcatt ttgaagatac tgtttttcat
    51961 gcaagagtgc ttgcttcaat gcttcttttt tctcgtccaa ctcgtaaaga caaaaaacaa
    52021 gcaggctatt agcctcaaag ctttctttca actcgtaagc tcgttgaaat aattcttttg
    52081 cctgctgata ttgctggttt gctagtgctt gttgtgccac tcgcattaaa ttctcatatt
    52141 tttctggaaa atcaatcggc ttcacgcagt gtcctcctct tgctcatatt cagctcatgt
    52201 tcaatagtag caaaacaaaa aaagcatgac aattaaaaat aactgaagca ccctctaaaa
    52261 gctagatttt agatctgact tttagaaggt gctttcaact gttactgctt tttattgctc
    52321 atcagctgtt gacttcctgg ttgaacgctt tgatcagatt gatcgtaaca acacttgtat
    52381 tagtgaaaaa atcgcccaga tgttgctttt tctgattaaa gactgctgga agatacccta
    52441 acatcagcaa aggaaatttt agaatgaacc ggcaagcacc ttctctaact aaaacggttg
    52501 accagctaag ttcttcttca tcaaagctga tgactcgaat accaaagatc attttcccaa
    52561 tcgtttggcc atgattgaat ttcgtcaata aaataaaata cgctagataa ataagtaagg
    52621 ctgtgaatcc gtagagactc agataagaat ctgaaaaatt aagccaacct agatttttgg
    52681 caataccaag tgttaaacgc gtaatagctc cgatacacaa aagatcgacc aagaaggcaa
    52741 acattcggat aaaaaaacct gcatagaaat acttaggaaa atcatttgct tttggcggtt
    52801 catttcttgt gtccgtatac tttttccatt tacgttgttt ctttttgatt tcttcgggcg
    52861 tcaaaggctc tctttcaaaa gagctcagta ccttttggga aaagttggag tcaggtccta
    52921 cagcaggctg ctgttgctta tctttttctt gttccttatt ttcatttgaa agctctgaca
    52981 tgcttactca cctccgtaat aatacattgc ttttggtgat tccgctgtcc ctagattctc
    53041 aagaacggat aagatctgac tagtctcaga aggcttcaat ccctggaatt ctgctagttt
    53101 acttcctaac catgtactag caaacccaga tggcgtggaa gaatattcta ctagttccgc
    53161 atcttcaagc tgttgttcct ttcgtatagc ttttaaagca tcttcaggaa atcccaattc
    53221 atcgactaaa cctacttctt ttgcttggac accatcataa atccttccat cagcaatttt
    53281 tttgacttct tcttcagatt tattccgtcc ctcgctgacg attgctacaa aacgactata
    53341 tgcgctgtcg atatatgctt gaagcaccgc ttcttcttct tttgtctctg gtcgcgttcc
    53401 tgaacccata tctttcaacg ctccgctttt aactgttgta tcttccacac ccagtttttc
    53461 tagaagtcct gaataattca atcctgacat gatcacacca attgagccgg tcactgtttc
    53521 ttctgttgca aagattttat ctgcttgagc agaaatatag tagccaccgc tagctgccat
    53581 gtttttcatg ctgacataca ttgggatatc atgttcttta cgaatcgtat ccaatttctt
    53641 agcgatttcc gcactttcgt agacaccgcc accaggagaa tttacttcta aaaagatacc
    53701 tttgactgtt ggatcttctt caatcgcctt cagttgttcc ataaaatttt tatgattgta
    53761 accttcagaa gcaaataaac ctgaagaccc tcccgcagca atcgttccat ctactgataa
    53821 ttttacgatt ttttgcgtac tgtctccctc ttccaacaca tttggagcta gttcttcatc
    53881 cccatataac caactgttca atccctcaat ttgatcgttt tcctgaggct tagtcaaact
    53941 agctgaaact aacgaaacca caaataatcc tgcagcaatc agaactgctg cccaacgtct
    54001 tttattcatt ctttctcctt tctaatacaa gttttttatg acgatcacag tttctgactg
    54061 ctcagaagtg tcgaaccgct ctgctttttc ttcccgcgca tacccggaaa acgactcttt
    54121 tgttaattca tcttctaccg taaactcacg agcttgagca acttccaagg catcaggtgc
    54181 gatttcttgg atgctatccc agccagtatc taaaaatatt ttcgtatcat ctgatacacc
    54241 agctgatgct tcgaatttgg acaattgttt ttttaacatt cccagtgtca tgatctcaat
    54301 ttttgaatcc atttttactc ctcctctatc cgattacttt ctattgtacc gaactcctga
    54361 aaaagatgat agataaaaac ccattatttt ttgacataaa aaggacattt gacaaagaat
    54421 ttgatatata ccaatttatt ttttattctt tttttgttga caagaacatt cgcaaaaggt
    54481 tatgatttta aatgtttcca gataaaaaac aaaaaggagg atagttatac cttttaggat
    54541 agcgccaggt ctgtgtaaac agatgacgag gagagggttt atcgaaagat tcggcggatg
    54601 gccctaggga ctgcactcta cagaaaatcc acaaaaaatg tctgcaaaga taaaacaaaa
    54661 ggattttcct gcagctggaa gcattcatac gaattcatta tagaaagtag gatgtttttt
    54721 atgtgcggaa ttgttggaat gattggatta aaaaatgtaa caccaggatt gattgacggt
    54781 ctagaaaaat tagaataccg tggttatgat tcagctggta tctttgtctc agatggcaca
    54841 actgattatc tggtcaaagc acaaggcaga attcaaaatt tgaaaaacaa aataaatatt
    54901 gatactacag gaaatatagg tattggtcat acacgatggg caactcacgg gcaaccttct
    54961 gaagaaaacg cccatccaca tacttcccaa agtggacgct tcgtgctcgt tcacaacggc
    55021 gtaatcgaaa actttgaaga acttaaaatt gcttatttag caaatgacca ctttattggt
    55081 gagaccgata ctgagattat cgctcatcta attgaaactt tcgcgaagga tactacaacg
    55141 caagaggcgt ttttaaaagc tttgcgggtc atcaaaggtt cttacgcttt cgctttgatt
    55201 gatcggacag cacctgatgt tatttacgtc gctaaaaata aaagtccact attaatcgga
    55261 ctcggcgatg gattcaatgt gattgccagc gatgcaatgg ctatgcttgc tcatacgaag
    55321 gaattcgttg agattgaaga tgaagagatg gttaccgtca catcagaaaa agttattatt
    55381 caaaattttg caggtgacat cgtagaacgt tcttcattcg aagcacaagt agatgcttca
    55441 gatatcgaga aaggtaccta tcctttttat atgctgaaag aaatcgacga acagcctatt
    55501 atcatgcgtc gtatcgctca aaaatacgta acagaagaca atcgtgtctc tcttgatcaa
    55561 aagctggtcg atacattgtc tgatagcgat cgaatctata tcgttgcttg cggaactagt
    55621 taccatgcgg ggttagcagg taaacagact ttggaaaaat tgacacagat tcctgttgag
    55681 gttcatttag ccagtgaatt tggctataac acacctcttc tttcaaaaaa accattcttt
    55741 attttcttga gtcaaagtgg cgaaacagca gacagccgcc aagtgctggt aaaaatcaac
    55801 cgtctcggct acccttcact tacgattaca aatgttgctg gttctactct ttcgcgggaa
    55861 gcatccttta cactgctatt gcatgctgga cccgaaatcg cagtagcatc aacgaaagct
    55921 tatactgcac aaatagctgt actaacttta ttagccaaag ctatcggtga taaaaaagaa
    55981 tttgaaactt ctcttgtatt cgatatcact catgaattga gtttagttgc taatgcgatg
    56041 gaatcggtca tcgcccaaaa agattattta gaagagttgg cagaagtata tctcagcgat
    56101 acaagaaatg ccttttacat tggacgtggt gctgactaca acgtttctct tgaagctgct
    56161 ttgaaattga aagaaatcag ttatatccag gcagaaggtt ttgccgctgg cgaattgaaa
    56221 catggaacga ttgctttgat cgaagaagga acaccagtga tcggaatcat cagtgaagaa
    56281 gtgactggtg cgcatactcg aggaaacctg aaagaagtcg aaagccgtgg cgctaaaaca
    56341 ctagttattg tctcagaggg attagaaaag gaaagtgacc agctggtgct accaactgtc
    56401 cacccttatc taactactct aaccacagtc gtaccgactc aattacttgc atattacgca
    56461 acgctattac ggggattcga cgttgacaaa ccacgtaatt tagcgaaatc agtgacagta
    56521 gaataataaa agaacaagga ccagtgtcag acatctgaca ctggtccttg ttctt
//
LOCUS       DACCWK010000005        54032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCWK010000005 DACCWK010000000
VERSION     DACCWK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.005"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(64..729)
                     /locus_tag="IVW65_000283"
     CDS             complement(64..729)
                     /locus_tag="IVW65_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899214.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(876..1481)
                     /gene="dhaL"
                     /locus_tag="IVW65_000284"
     CDS             complement(876..1481)
                     /gene="dhaL"
                     /locus_tag="IVW65_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7899215.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1509..2492)
                     /gene="dhaK"
                     /locus_tag="IVW65_000285"
     CDS             complement(1509..2492)
                     /gene="dhaK"
                     /locus_tag="IVW65_000285"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7899216.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(2494..2889)
                     /gene="dhaM"
                     /locus_tag="IVW65_000286"
     CDS             complement(2494..2889)
                     /gene="dhaM"
                     /locus_tag="IVW65_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7899217.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3043..3363
                     /locus_tag="IVW65_000287"
     CDS             3043..3363
                     /locus_tag="IVW65_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7899218.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3475..4344)
                     /locus_tag="IVW65_000288"
     CDS             complement(3475..4344)
                     /locus_tag="IVW65_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7899219.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(4440..5744)
                     /locus_tag="IVW65_000289"
     CDS             complement(4440..5744)
                     /locus_tag="IVW65_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7899220.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5741..6250)
                     /locus_tag="IVW65_000290"
     CDS             complement(5741..6250)
                     /locus_tag="IVW65_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899221.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6425..8560)
                     /locus_tag="IVW65_000291"
     CDS             complement(6425..8560)
                     /locus_tag="IVW65_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7899222.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8643..10298)
                     /locus_tag="IVW65_000292"
     CDS             complement(8643..10298)
                     /locus_tag="IVW65_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7899223.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10300..10614)
                     /locus_tag="IVW65_000293"
     CDS             complement(10300..10614)
                     /locus_tag="IVW65_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7899224.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10784..11044
                     /locus_tag="IVW65_000294"
     CDS             10784..11044
                     /locus_tag="IVW65_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7899225.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11058..11372
                     /locus_tag="IVW65_000295"
     CDS             11058..11372
                     /locus_tag="IVW65_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7899226.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11857..12411)
                     /locus_tag="IVW65_000296"
     CDS             complement(11857..12411)
                     /locus_tag="IVW65_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7899227.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12537..14465)
                     /locus_tag="IVW65_000297"
     CDS             complement(12537..14465)
                     /locus_tag="IVW65_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7899228.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(14771..15487)
                     /locus_tag="IVW65_000298"
     CDS             complement(14771..15487)
                     /locus_tag="IVW65_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7899229.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15488..17311)
                     /gene="glpO"
                     /locus_tag="IVW65_000299"
     CDS             complement(15488..17311)
                     /gene="glpO"
                     /locus_tag="IVW65_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7899230.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17325..18821)
                     /gene="glpK"
                     /locus_tag="IVW65_000300"
     CDS             complement(17325..18821)
                     /gene="glpK"
                     /locus_tag="IVW65_000300"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7899231.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18957..20417)
                     /locus_tag="IVW65_000301"
     CDS             complement(18957..20417)
                     /locus_tag="IVW65_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7899232.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20407..21702)
                     /locus_tag="IVW65_000302"
     CDS             complement(20407..21702)
                     /locus_tag="IVW65_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7899233.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22034..22711)
                     /locus_tag="IVW65_000303"
     CDS             complement(22034..22711)
                     /locus_tag="IVW65_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7899234.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22741..23832)
                     /locus_tag="IVW65_000304"
     CDS             complement(22741..23832)
                     /locus_tag="IVW65_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7899235.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(23933..24367)
                     /locus_tag="IVW65_000305"
     CDS             complement(23933..24367)
                     /locus_tag="IVW65_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7899236.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24951..26408)
                     /locus_tag="IVW65_000306"
     CDS             complement(24951..26408)
                     /locus_tag="IVW65_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7899237.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26405..28300)
                     /locus_tag="IVW65_000307"
     CDS             complement(26405..28300)
                     /locus_tag="IVW65_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7899238.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28358..30133)
                     /locus_tag="IVW65_000308"
     CDS             complement(28358..30133)
                     /locus_tag="IVW65_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7899239.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30183..31679)
                     /locus_tag="IVW65_000309"
     CDS             complement(30183..31679)
                     /locus_tag="IVW65_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7899240.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31873..33495)
                     /locus_tag="IVW65_000310"
     CDS             complement(31873..33495)
                     /locus_tag="IVW65_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7899241.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVSPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33945..35258)
                     /locus_tag="IVW65_000311"
     CDS             complement(33945..35258)
                     /locus_tag="IVW65_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7899242.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(35503..35727)
                     /locus_tag="IVW65_000312"
     CDS             complement(35503..35727)
                     /locus_tag="IVW65_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899243.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35747..36115)
                     /locus_tag="IVW65_000313"
     CDS             complement(35747..36115)
                     /locus_tag="IVW65_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7899244.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(36311..37022)
                     /locus_tag="IVW65_000314"
                     /pseudo
     CDS             complement(36311..37022)
                     /locus_tag="IVW65_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37040..37536)
                     /locus_tag="IVW65_000315"
                     /pseudo
     CDS             complement(37040..37536)
                     /locus_tag="IVW65_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(37573..38025)
                     /locus_tag="IVW65_000316"
     CDS             complement(37573..38025)
                     /locus_tag="IVW65_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7899245.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(38160..38765)
                     /locus_tag="IVW65_000317"
     CDS             complement(38160..38765)
                     /locus_tag="IVW65_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7899246.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEMKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(39215..39835)
                     /locus_tag="IVW65_000318"
     CDS             complement(39215..39835)
                     /locus_tag="IVW65_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7899247.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39837..40706)
                     /locus_tag="IVW65_000319"
     CDS             complement(39837..40706)
                     /locus_tag="IVW65_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7899248.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40729..41136)
                     /locus_tag="IVW65_000320"
     CDS             complement(40729..41136)
                     /locus_tag="IVW65_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7899249.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(41155..42444)
                     /locus_tag="IVW65_000321"
     CDS             complement(41155..42444)
                     /locus_tag="IVW65_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7899250.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(42471..42773)
                     /locus_tag="IVW65_000322"
     CDS             complement(42471..42773)
                     /locus_tag="IVW65_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7899251.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42798..44891)
                     /locus_tag="IVW65_000323"
     CDS             complement(42798..44891)
                     /locus_tag="IVW65_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7899252.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(45380..46108)
                     /locus_tag="IVW65_000324"
     CDS             complement(45380..46108)
                     /locus_tag="IVW65_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7899253.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(46254..48875)
                     /locus_tag="IVW65_000325"
                     /pseudo
     CDS             complement(46254..48875)
                     /locus_tag="IVW65_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(48989..50320)
                     /locus_tag="IVW65_000326"
     CDS             complement(48989..50320)
                     /locus_tag="IVW65_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7899254.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(50286..51764)
                     /locus_tag="IVW65_000327"
     CDS             complement(50286..51764)
                     /locus_tag="IVW65_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7899255.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(52069..53904)
                     /gene="tdc"
                     /locus_tag="IVW65_000328"
     CDS             complement(52069..53904)
                     /gene="tdc"
                     /locus_tag="IVW65_000328"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7899256.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        15292 a        11654 c         9113 g        17973 t
ORIGIN      
        1 aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt
       61 tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt
      121 ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat
      181 ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa
      241 atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata
      301 atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc
      361 tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta
      421 ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg
      481 gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg
      541 tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca
      601 tttttgccag tgttctttat attctttctt gatttgttca atcttatctg ttggatggct
      661 ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc
      721 aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat
      781 ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa
      841 aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat cacatctaaa
      901 agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc gattgcacgc
      961 tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg ttcttttgcc
     1021 tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac tggcagccaa
     1081 acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat acccgctgtt
     1141 ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac ctcttttgtc
     1201 gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc accgactttc
     1261 gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg atgatcggct
     1321 tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg gtctccatcg
     1381 ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc ctctgcaaat
     1441 tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc cttttatctt
     1501 gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc
     1561 tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt cataaaatta
     1621 ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa aacatcattc
     1681 atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag tacacctgct
     1741 tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt tgccagtact
     1801 ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc aacaccatac
     1861 tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac agttgctgct
     1921 cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag ttctttcaac
     1981 tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac aaggacagta
     2041 cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat atcatcatct
     2101 acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc catatcgaag
     2161 ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc actatcggct
     2221 tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc accaagcacc
     2281 gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg tccgcttcct
     2341 ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac acgggaagat
     2401 tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat ttcttcgaca
     2461 atattgcttg gatcattcat gatttttttc aatttattcc catcctttct ttccggcatt
     2521 ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga tggctgctgc
     2581 aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct gctcttcttc
     2641 caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa
     2701 ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg ggtctgatcc
     2761 gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt tttgcatctg
     2821 ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa gcaaaatact
     2881 ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt tcgctttcat
     2941 taatgaacaa ttcaagtata taataaattt ctaattttgg gaaaatatgt gtagtgcttc
     3001 ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac aacaacaacg
     3061 aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg tgaggtcatt
     3121 actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt tggcggccgc
     3181 tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc cgaaatggcc
     3241 gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga ctatgaagta
     3301 ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt ctccacgaga
     3361 taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt ggcactcaag
     3421 aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc ttttttattt
     3481 ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg ctaaggaata
     3541 actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg atttggatgg
     3601 ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt tttctcctgc
     3661 cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt tttttgcttc
     3721 aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt gactggccgc
     3781 tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa cctgatttaa
     3841 ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt ggacagcttc
     3901 cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat ttcccacttt
     3961 atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca atttgttttt
     4021 tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt gcaaatgatc
     4081 gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg aaatcgtttc
     4141 tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac cgttttcagc
     4201 accagaagag cttatggttg ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa
     4261 agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta acggcttcat
     4321 tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt attataagca
     4381 aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt
     4441 tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa aatcgttttg
     4501 actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa caaaagcata
     4561 tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac aatcagcgaa
     4621 gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta
     4681 aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat taatccagca
     4741 aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt ttgatcaatg
     4801 aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt gaaattcgtc
     4861 agccagactt ctgttccacc ttttgcccaa cgttttctgt gtcgatatag catcttcaac
     4921 gtcacaggaa cttccatgaa aaagataatc tcaggagcaa aaacagataa ccagtcattt
     4981 aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct gaatccgccc
     5041 acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc ataaagacca
     5101 ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt
     5161 tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt gaccgcagca
     5221 atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc atctggctcc
     5281 ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc aagtccgata
     5341 ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag attagcgtat
     5401 tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc gtctgtcact
     5461 aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt atattcgatc
     5521 acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac actcaaggga
     5581 acatctaccc attctttttg cttatgtttg tatataaaca cataacacca gacaccaata
     5641 aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaaatacat aatagacaaa
     5701 caagtatcta agaataaatt acctgtatgg gtgattgaga tcatttcagt tccaccccat
     5761 tttctttata caaatctgca atgaatgtcg tatctagatt tttctcttcg gagacagaat
     5821 aaaaacggac cgtttctctc tcttcttttg ttccaaaacg ttccgtatac acttcttcaa
     5881 gtaattcttg tctttttttc aatttttcat cttcatattc aaaagattta tttacttttt
     5941 tgtatagatt tcgattgttc cacctagtca atacaaatga ggtaatcagg aaactgaaaa
     6001 aaatcaatac aaaaaagccg attaagaaat acagcatttt cttttctgat aaaaaagcgt
     6061 acaaggaaaa atccttggct ctttctggtc tcgttaatgg gaaaaagacc catataaaag
     6121 gaagaacaac gatcatccat cctaaaagag ccactaacgt ttgccaaatc tttaaccagt
     6181 aattaccttt tgaaaaatac ttatctttca ccaaaattga tgcaacttct tctcttgtta
     6241 cttttttcat atcctcacct cacgtttttt tgttacgacg taggagttta ccatacattt
     6301 catttctttt cacataaagt gttcctaaat ttttcctaag aaaaagaaca aaacatttct
     6361 catcagcaaa tgaaaaatat tttgttcttt ttatcagttg ttctttttat tttttatctc
     6421 ttaattatgt tgctctttcc aatacgacaa tgcttttcat tcctctagat atcgccgtgt
     6481 acatttgtct gcgtcttctt tgctcatctc catcgttttc aaatggatat aagattacat
     6541 gatcaaattc cagtcctttt gctagactca aaggaatgat ttggcagttt tctaatccat
     6601 atgaattttt tttaatcgat tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa
     6661 ctgccgcttt ttctcctttt agagaaacca aaatctcagc tagtattttc aacaattctt
     6721 cagaggatcg aaaagagcgg tattcgggtt cttttccttg cggtcggatc gatacgatgt
     6781 cgatcttctc ttgatccaca gcataggttt tgaaaagctc tgtgattgct ccgctagaac
     6841 ggtaactatt tctaagatca taccgatgga tcggcagatg gtagtcgtcg aaacaacgca
     6901 taatatcagc aaaagtagtg gaagaattaa agattgcctg attctcgtca cctactaaag
     6961 taaagtgagt ttttggaaat aattcgatca atagacctaa ttgtgccagg gtataatctt
     7021 gtacttcatc aatcaataga tagcgaaatg ctggtacatt taccttttca acaagtaaat
     7081 gacgaatagc taatataatg actgcttcat caacagtacg tgtgggttgc caagcataag
     7141 ctgagccata tcgtttttca taaattcttt caaacaactg atgctcatcc acccatgcca
     7201 tttcttcgat ttgtctggtt atttttcgat attttttttg taaaagtttc cttgcataag
     7261 ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc aactgcctat
     7321 cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt ttcaaaagat
     7381 gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg atcaatttag
     7441 gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg actgtttttc
     7501 catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga tctgattgtt
     7561 ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat acattttctc
     7621 ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa agctgtgaaa
     7681 ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt aggacattcg
     7741 aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg gatgtcatcg
     7801 tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag gttttaccac
     7861 ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg attgtgtttt
     7921 gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct tggcctaaga
     7981 cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg accaaacgat
     8041 ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta ttttgacgaa
     8101 ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc
     8161 aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg
     8221 attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag ggacttttca
     8281 ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata ttcatctgat
     8341 cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct aagtaactgt
     8401 caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct tctatttttg
     8461 ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga tacacgttct
     8521 tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc ctcttttttt
     8581 aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct ttttaacgag
     8641 tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa agaaaatgca
     8701 ccatcaaggt tttttacgtg gtagccagac tgcttcaaga ttctttccgc tagatagcta
     8761 cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc atttaatcgg
     8821 tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt ttccaattct
     8881 tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg caattcatgc
     8941 cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc tagcatgttt
     9001 actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc cggtaaatcg
     9061 aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt atccacacct
     9121 tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa gatcaacttc
     9181 agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga tacatgaatg
     9241 acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt ggatgcggca
     9301 gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct tcgagaaatt
     9361 ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc aagagaaata
     9421 agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac cgcataaata
     9481 tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat gcctaattcg
     9541 attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa aataactaaa
     9601 tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca
     9661 gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt ttctataaaa
     9721 gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat caccatgact
     9781 tctaaacctc ttctttttag gttttctgcc atttccaatc cgatgaatcc tgcaccaacg
     9841 atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat atctggtaca
     9901 tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg aacaactggt
     9961 tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg accctcatgt
    10021 acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt tggacgcaca
    10081 tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt ttcccgttca
    10141 ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac atacggtcct
    10201 ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt agcagcagac
    10261 attccacctg ctacacctcc gacaataacg attttcatct tatttccctc ctactgttcg
    10321 atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag atttctttaa
    10381 ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga cggtcttttc
    10441 tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc cttttatatg
    10501 cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt ccaaaagctt
    10561 ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat tcattttctg
    10621 cacctcattt acatttttat aaatacctca tggggtatat taaacaggag ctttattaat
    10681 atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca taaaataccg
    10741 ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga ttgataataa
    10801 aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat
    10861 cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc gttctagtat
    10921 tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg agaaacctga
    10981 tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga ttattaaaaa
    11041 gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa aaatggtcag
    11101 aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg attgtcgtat
    11161 ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa ttcttagatt
    11221 tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat atttcagaaa
    11281 gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta gttgaactgc
    11341 aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca gaagtgatca
    11401 gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa
    11461 ggttgtgaaa acgctgtcct gcatcaagta ataagaacag ccaaacaaaa atagcttctc
    11521 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg
    11581 aagaggttgt gaaatcagcg gtttgaccta agtattagag aaaaaaacgg gaaaatagct
    11641 cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttac
    11701 caagaggttg tgagcctacc atttaactcc gaatcacaag aaataatttc aaaaacaact
    11761 tctcctgttt ttggtttggt tggcttatta tcgagaagta gctactggga gtctatttat
    11821 caaaaaaagg aatgcgacat aggtcacacc ccttttttat tttctttgaa acatctttcg
    11881 gtactcgaca gcatacaaga caaaagattg taaaagaccg taagtgatga cggcaaaaat
    11941 gactctcacc cacagcgttg gtaattctgg gaaaagcagc aaaaggatca tgccaacata
    12001 gatgacagca gtcgctactt tgccatacca cttcgctcca cttaatttga tatttttaga
    12061 tagcccgatt aatccagcta caaacataaa gccttctttt atcaagaata gaccaaacaa
    12121 cagccaaagc cacggtcgat accatgccat agacaaaatc agaaaaagtt gtgtcaattt
    12181 atctgcaaac ggatcaagca ccttccctaa gtctgttacc tgattatatc tccgagcaat
    12241 gaccccgtct agataatcag acaaacctga gacaacaatg accgctcctg ctatataaaa
    12301 tgactgtctc agcacaaaat aaagataaat cggcagcaaa aaaatacgga atatcgataa
    12361 aagattcgga atcgtttgta tttctttacg ccagtctgtg cttaatttca ttttatcgcc
    12421 ctacctcctc agcactcttt tctttttatt ttagcttaca cggggaataa aaccaatgat
    12481 ttcactgttt ttatttttct ttacagccac tgatattatg agctaatttg agataatcac
    12541 ttgctgtttt catcgtttac tcttctttct tcctgtcgtt tgcgcatttc ttttactagt
    12601 actttatgag ctttttttga tcctcgctct aggtcacgct gccgtttacg tgttttataa
    12661 tcgataaaca gactgtctaa atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc
    12721 agacgaacac gctgacgagg cacgtggatt atttttttgt ctaaatatac atcaatcgta
    12781 tcttcaccta tatcttcata aactaacgct tctttttggt attcattgac aaatatccga
    12841 tcaccttttg caaagagctg gcgttctttt ttattctcct tcctatttgt aactgctgtc
    12901 ttaggaagaa attctttttt ctgtgtccga taggcttttt ccgccaagta acgctctgct
    12961 tgatggatca atttcatcga catatccatt ttatgagcaa tccacaaagc ttggctttct
    13021 cctgtttctc caacccgcaa ctgatattta ggacgaagtg cttcccgatc gaaagccata
    13081 gcagccggca caaaatcttc atgatctccg gcgaatttct tgatttctcc ataatgggtc
    13141 gtggcaatga tcaatgcccc ttgctcatac atagattcca tgattgcgat cgctagtgca
    13201 gccccttcat ttggctctgt tccactgcca atctcatcta aaagtaccaa cgtattcctt
    13261 tttatctttt tcaaaatagc agcgatgttt tgcatatgac cagaaaatgt actcaacgca
    13321 ttttctaaat tttggtggtc accgacatcg acgaaaatct catcaaagac agcaagctct
    13381 gtcccttctt ttgccggaac ttgcataccg aacatcgcca tcaaagtcaa taaaccgact
    13441 gtcttcaaca cgactgtttt gcctccggca tttgctccgg tgataatcaa gcctcgatag
    13501 tctttaccga tttcaacatc caatggtacc gcatgctcag gtaaaaatgg atgtctgcct
    13561 tgcttgataa tgatatgttc cgatttattg attctaggtg tgcttccatt gatttctcgg
    13621 ctaaattttg cccgagcaaa gatgatatct aacaccgtga tcgtttcgat caataaatcg
    13681 atcacttctt cgttttctgc gatagcacca gtcaatgccg ccaggacttg atattcttca
    13741 ctgatttctt cagcttttaa caactgatag tgttcattca atttagaaat cacagttggt
    13801 tcaataaaaa ccgtcgttct tttatttgat tcatcgataa tcgttccatc tattttattt
    13861 ttatagctgg ctttgatagg aatcgtatag tattcgcctt tttgaacaat cattgcttct
    13921 tggatcattt ctttgttttt cggatgacgc aagaatttca gtaattttga ctggatttct
    13981 ttttcgatta gttgtaattg tttgcgtact ttacgcaaat tacgtgacgc gtcgtcactg
    14041 actttattat tttttatttt ctggtcaatc aactcttcta catttatcaa atcaggaaga
    14101 tgtttgctat aagcaaacaa caagggtgtc tggtattgat tcttatcaaa aaactttgtg
    14161 atcatccgac tgcttctaag aaaatccgca tattcgatca aatcggttgg ctgcagaatc
    14221 aacccttttt tgacttgttc tgtcagtgca tcgatcctcg gcaaccccat aaatggaaca
    14281 tgctgactgc tttctaagat caagcgtgct tctcttgttt cttcttgacg atccaagaca
    14341 gtttgtaggt ttgtcgagac cgtcatatcg ctgattcgtt ttttgctata gtttccaatt
    14401 gctcttgctt ggatttcctt tttgatccga tcaaattgta attgactaat catttcgttg
    14461 tccatattct ctccttttct tgaagagata cctgtcccaa aataaataag ggcctagaga
    14521 taaataaaag gatagaacac agcttaaaaa agtcatgtcc tatccttatt agaatacaaa
    14581 gatattgttt tcctgacagg taccgtttca taagcacacc aaataaactt tgtttattgg
    14641 ctattcgact tactgattag ataccagcta cactcacaaa acatacccca ttcttctagg
    14701 tcttatttat tttccactta actatagcaa gacacatttt ggttgtcaaa ctttagtatg
    14761 ttttatagat ttacaccatt aatgaataga cacctaccgc gataattgct ccgatcattg
    14821 gacctgcgat tggtacccat gaatatgccc aatctgaagt tcctttatta gctattggca
    14881 ataccgcatg tgctaatcga ggaccaaagt cccgagcagg gttgattgca tatcctgttg
    14941 gccctcctaa tgaaagaccg atggctaaga tcaatccacc tacagcgaat acgttcgttc
    15001 caggtgcaaa ttcgttttgt ccaaaagcta gcaacccaag aactaataca aatgtaccaa
    15061 ttaattctgt gatcacattt gctggataat tccgtatagc aggacctgtt gcaaacgttc
    15121 ctaaaatcgc gctttcatct ttcgttgcat tccaatgtgg caaataagaa agccaaacaa
    15181 gaatggcccc tgcaaaagct cctaaaactt gcgcaataat gaaaggaacg acaagattcc
    15241 aactaattgc ccctgtcatc gccatagcta gagagactgc aggattcaaa tgagctgggc
    15301 ccatgaatcc agaaatatag acagccattg ttacagcgag tccccaacca aatgcgatga
    15361 caatccagcc agaagcctgt gctttacttt tgtttaaatt cacagccgca cacacaccat
    15421 ctccaagcaa aactaggatc agtgtcccaa taaactcccc catgatttga gtcatatcac
    15481 tgttcattta tttctccccc tttagttccc gcagatctga ttctgcaatc aatgcttcta
    15541 aacgtttagt ctgttgcgcc ttctcctctt ctgtccattc aaaatatcca gcaattgcat
    15601 cgatcacagg ttgcttgatc tcatcaagct gatctcgttc aaacaacaga tgatttgtac
    15661 gacgaatgag ataatcgcct ggggccaaaa ccatttcttc ttctaaacca taacgtaacc
    15721 gagctgactc agcgagactc aaaccaggat aaggtgccat ttcttttgct aattcaaaga
    15781 tacgtcgggc attagtcccg taaaaatcag cgatataagt agcatcttct tcctctaaac
    15841 cggctgctac tccttctttt gctaattctg tcactgtttc ttctaattta gttggatcga
    15901 agtttccacc tgaaatctga tattttttag agtcgatttc tttcgtctct attccgtatt
    15961 cttcttttaa taattggcgt atcaatcgca aagctccttc tgccatctta cggtaatcgg
    16021 tgattttccc accagataaa gtcagcaaac catctggttc tctttctaaa gaactacctc
    16081 tggaaatcgt agaaggtgct ttttcatcac ggctgttttc caaatggttc aacacgtctt
    16141 ctacttcagc acgagaaact ttgttttcct tatattcact tactgtatcg acaactttat
    16201 tgaagctctt gtctgaaatc gatccattat ctccaccatt gtaatcagaa ccagaattac
    16261 caatcaacag tggacgaagt cctgcccagc ttgcttcgat atcattcaat gtgatatttg
    16321 cttctggata gcgatgattg atcacatcca atagataatc tacatcttct tgtgtgactt
    16381 tcggatccgt aaaatctcct tggtaatccg tatctgtcgt accaaagtaa gtcttgtttt
    16441 ctcttggaat agcaaaaacc atccgcccat cttgctttcc tgtatcgaag tatgtgggtt
    16501 gcggtacagg cagttttttc gcatctacaa ctaaatggat ccctttagtt ggacgcattt
    16561 taggagagac tggacgcgta aaattcaagt ttcgtacttt atctacccaa ggaccactcg
    16621 tattgatcac taattttgct ttgatttcga tcacttcatc tgtcagcagg tcacgagctt
    16681 tgacgccaac gatttgatcg ccatcatata aaaagccaac cgctttcatt ttacttacta
    16741 gataagcccc atcttcggca gcctttttga tattatcaat cactaaacgg gcatcattgt
    16801 tgcggaaatc tagatagaca cccgcgcctt ttagcccttc ttttttcaaa aacggttctc
    16861 tttccaatac ttcttctggt gtcagcgtat aattctcata tttggttcct gtcacatttg
    16921 ccagtttgtc ataaaggtcc attgctactt tgaccgagaa catattgaag gttgttgctc
    16981 cttcgccctc atagattggc aaaagcattg gatctggttt tgggatgtgt ggggcaatac
    17041 tttgtacgac tgcacgttca ccaactgtgt cagctactac ttccacatca aatgtcttca
    17101 gataacgaat accgccatga acaagtttgg tcgagcgaga ggaagtccct tctgcaaaat
    17161 cctgcatttc gatcaatcct gttttgattc ctgatgctgc tgactggact gccacaccgg
    17221 caccagtgat tccgccaccg atgatcaaca gatccaattc ttcttgctgc attttttgaa
    17281 tgctatcttg tcttgtcttg tttgaaaaca tatggatttc ctccttattc aaatgcttgg
    17341 gtagctttta ctgctttttt ccaaccgttg taaagttttt ctcggcgctc ttcctccatt
    17401 tgaacttcaa atagcttgcc agcttcataa aattcgcgga tctcgtctgt atctttccaa
    17461 taaccgacag ctaatcctgc taaaaatgct gcccctagcg ccgttgtttc caaattttct
    17521 gctcgttgga tcggtacatt caagatatct gtttggaatt gcatcagata ttcattgttt
    17581 gctgcaccgc catctacttt caatacagga atcttgattc cagtgtcatc ttgcatcgta
    17641 tcgatgatat ctctcacttg ataagcaatc gactgtaaag tagctttaat gatatcttct
    17701 tttgtcgtac cacgtgtcaa gccaaacata gcccctcgtg ctgcctgatc ccaataagga
    17761 gcacctagcc caacgaatgc aggaacaaca taaacctcat cttcgcttgt agagcgtttc
    17821 gctgcgtctt ctgaatcgct agctttttgc aacatttgaa gtccatctct cagccattga
    17881 atcgaagaac cagccacaaa aatacttcct tctaaagcat agtaaacctt tccatttatg
    17941 ccgtaaccaa ttgtcgtcaa taaattattt ttggataatt gcggtttttc accggtattc
    18001 atgacaataa atgaacctgt accataagta ttcttgacca tgcctggttc gaatgccatt
    18061 tgtccgaata atgcggcttg ctggtctcct gccatccctg aaattggtgc ttcgcttcca
    18121 taaaaatgat aaccttgcgt ataaccataa acttctgagt tagaagtgac ttttggtaac
    18181 atcactcgag gaatatttag taaatctaaa atctcctgat cccaatctag ctgatggata
    18241 ttgaacaaca tcgtccgact ggcattcgag taatcagtaa catgtgcttg tccatcggtc
    18301 agtttccata ctaaccaaga atcgatcgtt ccaaacatca attcgccttt ttcagctcgt
    18361 tcttgcgcgc cttctacatg atcaagaatc cagcgtatct tcgttgctga gaaataggca
    18421 tcgatcacta accctgtttt ttcgtggaaa aattcttgat agccttcatt ttttaagtta
    18481 tctgcaattc cagctgactg tctggattgc caaacaactg catgatagat tggacgtcct
    18541 gtatcttttt cccatattac tgttgtttca cgctggttcg ttatgccaat cccagcaatc
    18601 tgatttggtt tgatccctga ttcgatgaac gctccggcaa taactgactg tacagaattc
    18661 cagatttcat ttgcatcatg ttccacccaa ctttcttgcg ggaaatattg cgtgaactct
    18721 ttttgagaac tgccgatgtg tcttcctttc ttgtcaaaga taattgctcg tgagctagtt
    18781 gtaccttggt caatcgccat gatatacgtt gattctgtca ttgtgattcc ctccattata
    18841 tgcaaacgct ttctttaact actcttatca tactactgtt ttaaaaaaag aaactgtcaa
    18901 aattttattc tttttttctt tttttgacag ttttttatta tgaagctatt ttttaatcaa
    18961 ttcttttctt ttttcagcgt ctctattctt cttttcaatc gaataatgtc gtaaggtgac
    19021 gcaaattctg acagataata ttcacactgt tttttgtttc tctggcaaaa tgtgatcacc
    19081 aaatcatatt ctttaccttc ttcataactt ccaatctcaa taccgccgat tcccctcagt
    19141 tctctgatca gatattgttg aaaagcaata cgataaatcg gcagactttc taaatcgatt
    19201 ccaatcacta ctggttttgc aatataaaaa tcgatcatca aaagaacatt ggcatactgg
    19261 atcatcaggt aactaagctc actatcttca gctcttttcc tcggcaattg ctgcaccgct
    19321 gtttcctgta atttcgccag taactgtgtt aaggaatgcc cgagcatttt ctgctgctgt
    19381 tccaaaagcc gatcacgctc atagacttcg atctttccaa caaagaaaaa ccattcattg
    19441 ttgatctggg aaatttggta gcctactgcc ttttcttctt tgattttcaa acgacgagga
    19501 cgataatgcc ataacatcgt ttcccgaatg taagtatcta aaacagcggt tggtaacttt
    19561 ttgctccgtg tcaaatcata ctgataaaat atttcttcgt ctactatcga aaaagaaaca
    19621 aaagagctgt aaaacatcat tgtttcgtat acattcagtt ctgctgctgt tcgactaaga
    19681 tgcatcacga tgattggatc gattgcttga taaagccggt ccgactgata gagtaatttc
    19741 ttttctttca aagttgcgta cgtacttttt tcattcaata gtcttttttt cgtaatcccg
    19801 agccagcaag caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa tacacggtcc
    19861 aatcctagaa tgaacggtct tttttctggt gtattttgca aaaaaagcgg tcgctgatct
    19921 tccgggatat attggaatag ttcataataa aaatatcgta tctgtagctc ttctccgtat
    19981 aattgaccgt ttttgatttg taatccaaat tcctctaaga gctggttcaa ttctttgatc
    20041 tttcgaaaaa cagaagactc actgatatta aacgctgtcg ctaatcgaac gatggatact
    20101 tgctgatgtt ctaataaata gacgagcatc ttaaacttca agctttcctg caataaaaac
    20161 aatagaattt tttccaaact tacatcttcg tccaacacaa gcagcgcctg atactcattt
    20221 cgctgtactt ctactttttt ttccatcatt cggccaatca attcaatatc ttccaaatat
    20281 tgatcaaacg aggcttttga cagttggaca tgctctctca aaccattgat agaggctgtt
    20341 cccccttgca gaacaaggtt tcgcaaaatc aggatctgta actgctcttt tctttctaat
    20401 aatgcttcaa tcttcatagg cagtattcgc cacctttgac cacaccatat ctggcacacg
    20461 gtattctggc tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc atccttcttt
    20521 tactgcaatt gtcagttggt caagaaggtg gaacatctct tttttgcttt ggagctgtgc
    20581 tccaagaatc tttttttcag aatcaaatag aaatttgagt ttaactttgc tttgatttat
    20641 tgctgtaagc ttgtagtcta cgtatcctgt ttcaattttt tggggataaa aaatgctctc
    20701 ttcttctgtc aaacctacgc ttcctaagaa atagccaaac cactgattgc cgactggacg
    20761 ataggttccc tgatcctttg tctgtgagcc actaattgtc ttgctgacat ttcgtgctgt
    20821 acggacagcg ttatttacta gagaaacata cattttttca tttgttggac gaaaagttac
    20881 ttggatcgca tcgccaatcg cataaacgtc tttttgagac gtttgcaagt agtcatctac
    20941 ccagatcgtt ccatcatcat tcaatgttaa ttgttcttcc cacatctgat tgtcggggcg
    21001 agaatgatta gccagcaaaa caagatcata gttctccttt cttttttccg tctctacaat
    21061 agcggtttca ttttcggtca aagacacaac ttgttcttct aaaaaaagtg aaagcccttg
    21121 atttttcatt tcctttacta acggttctgt catttcagga tctagataac gaaaaagaat
    21181 tgactttcgg ctttcgtaaa gatggatttg tttttttctt gatgccaacc cttcagctat
    21241 ttcgattcct gcttgtcctg caccaataat tgcaattttc tttgcattca tgatcttttg
    21301 ctgaagagcc gttacgtttt gtatcttttt aactgggtga ataagcgttg attctgcaga
    21361 aatatttcga aacttttgat ttgaaccagt cgctagaatc aaacgatcaa aatccaattt
    21421 ttttcctcct gctaaaacga cttttttttc tttcaatcct attactgtct tttctgtgta
    21481 aacatggatt cgatagcggt cagtcagttc ttctttcgtg atccaagaaa gttgtttttc
    21541 actggtaacc tttcctttta atatcaaatt gaacccgctg ggaatccatc ctaacttcga
    21601 ttgcttttca atcaaatata tttcacttgt tggcatcaat tggcgaagat aaatagccgc
    21661 atgaatccct gcaaacgacc cgccgacaat tacaatgttc atctgcattc ctctttctta
    21721 taaggtgttc tactgctaac ttctcaatca gaaaacacag tattcataac tcgctttcaa
    21781 ccttatgata catagcgatg cagcagaatc aaccctctcc atcataccgt gttctaggtc
    21841 tgcttctcaa taatgtacta gtggacctaa ttgaagagta tcatttctga agagatgttt
    21901 caaaaaatgt actcttataa aagaaaaaaa ctcgcctttt acgtacctct gtccaaatag
    21961 gaaacagaaa agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt tagaatagac
    22021 acatcattta tttttaattc tttaatgctt caacaactgc ttcgcctaat tgatgagcag
    22081 attgaggatt ttgccctgta agaagccgcc catcaatttg aaccacttct gaaaaagctg
    22141 ctccagactg atagtttgct cctttttctt tcaaagcatc ttctgctaag aatggtactt
    22201 cattcgttgt accatttgct tcctcttctt cattactgaa cccagtcact gtacgtccag
    22261 ctaaaataga ttttccagac tcatctgtaa ctggcaacaa tcctacaaca ccgtgacaaa
    22321 cagctgagac cagcccttca tttcgataga tttgtgaagc gactttccca agatctattg
    22381 cttctggaaa atcccacatt gttccgtggc caccagcata ataaaccgct gcataatcat
    22441 ttgcatccac atcatcagga cgtaaggtat ttcccaaggc ccgatttctg aaatcttcat
    22501 cttcataata ggcccaatcg atttcatcca tattcaaaac actttgcggt tctaaaggta
    22561 catatccgcc tttaggactg acaaaatctg catcgatccc attttttact aatacatcat
    22621 aaaaatgagt cagttcactt aaccacaaac ctgtaggacg ctcgatcttt tcgtactttt
    22681 ctacatttgt taatacgatc aatactttca ttttcccatc ctctttctat aaaaattgcg
    22741 ttattgataa cgtgctttca agaaattata taatgggctt ggcaaataaa gctctttata
    22801 tgcttcttca aaaggtttcg ttacaaattc atcctcacgg ttttccaata atttctctgc
    22861 ccagtcagga tcgacgatca tttgctgtcc aattgctgca agttctgcat atttcaatac
    22921 ctgctctaca tcttcgcgat tgcggatccc accgacacca actaaaggga tctttccttg
    22981 aatcgtgtta tggaccactt caagaatcga tttatcgttg tatttatcag aacgagctat
    23041 tcgatcatac atatttaatg aaacatgcag ataatctagt tttgactcac gtaatcgttc
    23101 aagaagccag atcgtgtctt caaaacggat acctggtgtt tcatattctt ctggtgagaa
    23161 acgataccct acgatgaatg gttttttagc atattgttca accgctgaga aaaccgttgt
    23221 taataattct tcaataaaac gataacgatt ttccaagcta ccgccccatt gatcctctcg
    23281 tctattcgaa tgcggagaga aaaattgttg gatcaaatac gtatttgctc catgcaactc
    23341 gattccatca aatcctgatt caatcgctct acgtgtggct ttgccaaatg cttggattgt
    23401 ttcaacgatt tcttcttcac tcattgctcg tggtgtttcc gcatcaggac gttttgctgc
    23461 aactgcactt gcagatacag gctgttctcc agataaagtt gctcgacttg tcattcgtcc
    23521 accgtggaaa atctgaataa tcgcttttgc gccttctcct tggattgctt tcgctagctc
    23581 acttaatcga ggaatcattt catcatgagc gatgctgagt tcacctggcc agccttttcc
    23641 gatatcttgg acatttgcag caccagtaat aacagcacca agacctttag aacgttgttt
    23701 ataataagtg atctcatctt cggtgatcac tccattgaaa aagctgagtt gtgtcgtcat
    23761 aggcgacagc atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa ttgattgatc
    23821 aaattgtgac attgtcattc ctcccataat tcgctctgat taacctatcg agaatcatct
    23881 atcattaaac cactcgatta gttgtacatg caactaattt gcttaaaaaa ttttattttt
    23941 tttgtaactt atctgttgca tcattcattg tctgggctaa ttctttactt gcttcatttc
    24001 ccaaaactgc tttgtatgac tgatgaagtt cttcccagct accatcgact tccttagcaa
    24061 attggcgtcc ttgttctgta agataaacta atgacaagcg accttcgcta actgttttga
    24121 cgtatccttt atattctaat ttttcaacaa aacgagcaat cgttgatggg gcggaataga
    24181 gcatttgtgc aatatctttt tgtggtattc catctttttc ttttaatatc aataaaataa
    24241 aaccataagt tggtgccaga cctgtccttc taaacgcttc atcagctaat ttattcaaca
    24301 tgcgatctag ctttttcacg ctaaagaaaa gacattcatc taacgctttt tcaattggct
    24361 tttccatttc atcccctcca ttggcgtaca tatcatagta tgatttttat tttttgtcaa
    24421 aaaacacgga ataatgtccg cctcctctgt ctagatttga aaaaaagaag tagccatctt
    24481 caaagaattt cggcttgttt tcgacaaaac tccttctgga acaccgtgaa tcagaggttg
    24541 tgaaaaagtc gtttagtttt gagtatgaag aaaaattttg tgaaaatggc tttccacatt
    24601 tttgcataaa tttggcttaa taccgaaaaa ctggcttttg aacaccgttt atatcagagg
    24661 ttgtgaaaaa gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
    24721 attttgtcgt tttttgagct aatgccgatg gtcagctgat tgtacaccgt ttattgagag
    24781 gtcgtgagcc tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca
    24841 tgttttatga ctatggtgac ttgtgatcga ggagttggca ggagaatact gtttattcgt
    24901 aaagaagggg ctgtgacaag acttttgtca cagtccctta tttcctttta ttataggatt
    24961 tcgccatttg attggatgac ttgtctgtac caatcaaatg attttttctt taaccgtttc
    25021 tttgtgccat tcccttcgtc atcttgatca acatagatat atccataacg ttttgacatt
    25081 tgtgaagtag aagcgctgac gatatcaatg cagccccacg acgtataacc aaataaatct
    25141 actccatctt cgatagcttc cttcatctgc tcaatatgct gcttcaaata gtcaatgcgg
    25201 taatcgtctt gaacggttcc atcttcttct actttgtcta tagctccgag tccgttttca
    25261 gctatgaaca gtggtttttg atacctatca tataattgat tcaaagcaat tctcaaaccc
    25321 ttaggatcaa tctgccagcc ccattcactt gatggaagat acggattttt aattcctcca
    25381 gttattaagt ttccttggac cccttccaaa ttttctggat gagcactgac tgcactagac
    25441 atgtagtagc taaaagaaac aaaatcaaca gggtaggctg ctaagatttc ttcatcacct
    25501 acttctattt ttaagttgac acctttttct tcaaacattc gttgcgtata cgctggataa
    25561 tatcctcttg cttgaacatc actaaagaac aaagaagttc tttgctgctc aattgttttt
    25621 tgaatatcat ccggatcaca agaatacgga acaagaagct gatagctgat catacaaccg
    25681 acttgtgaac ctttgataat ttcatgacaa gcttttgtag ctaaagcact tgctataaac
    25741 tgatggtgtg caccttggta tttcgcttgt tccaaatggg gatggttttc ttcaattact
    25801 cctacgctaa caaaagggtg atgttttaca cagttgatct cattgaaagt catccaatat
    25861 tttactttat ttttatagcg tttgaatact gtttcagcga aacgaacgta aaactgaata
    25921 agctttcgat taggccatcc accatattct gtaaccaaat ataacggcat ttcataatgg
    25981 gaaagagaaa cgactggctc aatcccatat tttagacatt cgtcaaacac ttgatcataa
    26041 aaaaataatc ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat tcttgtccat
    26101 gcgatagaca tccgatagca tttaaaaccc atttcagcaa ataaacggat gtcttcttta
    26161 aagtgatgat agaaatcatt tccgtgtctt ttaggataat aatacttgct gttgggatcc
    26221 tgcgccgctt ttacttgctg atgcgtcatc atatgccatt gatctgtcca tttttcttta
    26281 ggtaaatcgc tgtcgaatgt atacacatca gacacagata atcctttttc atctacatcc
    26341 catgcacctt cacattgatt ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat
    26401 tttttcatat cgatccccct aaacttgcta gttttcttgt ttgattattt ttcttatgac
    26461 tagtaatgat atttttgaat ctactactat ttgtaacaat aaccattgtg gtcggatcat
    26521 atccctcttt gattaattct tgcaaatcta cttccatcaa taaatcgcct ttacgtactt
    26581 tttttccatc agatactgca ctttgaaagt atttgccatt taaattcact gtatcaatac
    26641 cgatatggat cagtatctct gctccttctt cactgactac cccaattgca tgtttcgttg
    26701 gaaaaactgt tgaaacttcc ccgtttacag gactgtagat ttttccgtcc gttggaatga
    26761 tcgcaaaccc ttctcctaaa gatccactag caaatgttgg atcgtttact tcagataaat
    26821 caattatttc acctgctgca ggagtactta acactatatc ctttgaggat tcttcttcga
    26881 tttcatcctt gtagccgaaa agaaaagtta gcatacagga actgatcatc gctaagaaat
    26941 aactagcaac tagccaaaca aatccttcac caatataaat cgggaaagac aaaatacttc
    27001 ttgtagcaac tgccaaagca ccggctcctg cggcaccagc tactccacca aatatagctc
    27061 cacatataca agcaatgtaa aatggtcgtt tatatttaag agtcacacca taaacagcag
    27121 gttcagtgat tccaaataat gctgtgataa atgcagataa agataaactt tttactttct
    27181 tatttttcgt tttcaaaaat accccaagta ctgctcctgc ttgcgcagct acggcagtca
    27241 tacatgctgg acctagcgta tcacgaccgt agaaagaaag attgttggtc attactggta
    27301 caagtcccca atgtaaccca aaaatcacta acacttgcca aaatccgccg ataacagcac
    27361 cagataaaac agggctaaag ttataaacag ctaaatatcc agctgcaatc agctgacttg
    27421 cataatctgc aattggacct acaactaata aagtaattgg gaccatcacg atgatacaaa
    27481 tgaatggtgt caataaattt cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa
    27541 tataagaaag aacccataca gctaaaattg ctggaataac cgtacttgta tatcttgcta
    27601 gaatcacggg catccctaga aatcgcaagg ttatgctatc actatatgca ctgacaatcg
    27661 taggataaac gagagctgct gcaataacca tagcgatgaa acgatctgta ttgaactttt
    27721 ttgcagcagt atatgccaaa aatataggca agaaatagaa aaatgcatca gctgctgcat
    27781 tcaaaatgat ataagttcct gattcagtag ttaataaatt caaactcgta cataaggcga
    27841 gtactccttt gatcaaacca cttcctgcca agacgcctaa agtcggggca aaaacagcgg
    27901 cgatcatgtc tataaaagag tttaaaatgc cttttttctc agttcctctc tttccactag
    27961 atgcatcatc ttctagtttc atttgtttgg ataatacgtc atacacttca tttactgcat
    28021 ttcctactac gacttgaaac attcctccag cttcgactac agtgatcaca ccttctagtt
    28081 gctctacctt ctccttatct gccttcttat tatcttttag ggtaaagcgt aaccttgttg
    28141 cacaatggta aacattgctg acattttctt cgcctccgac aaatcggaca atatctttcg
    28201 ctaattgtga atagtccatt tttacttccc ccttgttaat taatgccgta gcaatccttc
    28261 ttacaagata agtatagttc ttagaaaacg gtttctccat tcaattagta ccccttctaa
    28321 tagcgaacga aactgtacaa tagtgtgtat ggtttactta gccgagcaaa tcactcactg
    28381 cttgaaagaa aacaagtata tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct
    28441 tgatcttcct ttgctgatgt ataagtggta tctcaagctg atactctgtg atttttgttt
    28501 ctacttggct tttgataaga cagcacaaca aagtctcggc atttaacgga agcataacta
    28561 ggtcagaact aggagagatt agttgctgaa tggaatctat ctttttatgg atttttattt
    28621 gattttcttc tttgtagtaa tgatcaagca gttttgtttt ctttttttct tcaactatgg
    28681 tcagcttttc acgcaaaatc tgataaatct ccgaagattc aaactcatct agaacactag
    28741 gtagataaat caaatcatcc cactcaaaaa taagctcttg ctcagtagtc aaaattgcat
    28801 catattccgt tattttgttt gattgatttt tgaaaagata cactggttct atccggcatt
    28861 ctttgatcca catgttcata ctttgttgaa tctgtttttt tagtgtattc aaaatactaa
    28921 ttggctggtt actaacaatc agtatctccc cttgttttcg aaaaggattc aacgcttcgg
    28981 caagatacaa aaccaaataa gcgctttcag ctaaattggg acgtaattga aaatgtattg
    29041 ggaagaaacg ccgaattaaa taaatttcta aaggataacg ggagatgatc ttttgtacaa
    29101 aatgattcat ggtattatgt ccggcatcca tcctcgtttt catttgtttg atatgggtta
    29161 gcaaaaattc tgtttcatgg aacaataacg aaacaggaag atctaaaaaa cgaataatcg
    29221 tttgctcaaa tgctcttaga ttcttctcta tttctttgtt tttttcatca ttaatcatca
    29281 aataatttaa ctctaatagt cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat
    29341 agggtagttc tgatgataac ttttttgtga gggtctctac catcggaata aataatgtat
    29401 acctgtttgt tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg agataccgag
    29461 aaaatcgtat gaagtcctct cctgaaacaa tataattata tgatataaga acctctttta
    29521 atgtcttttg gacagtcggc atgatattgg aaaaaattgt actgatttgt tgaggcaacc
    29581 ttgcgtgttg gatcaccgtt tctgttccaa caagagataa gaaaattctt tttgaatttt
    29641 cttcagcaat gatttttagt ccttttgtcg acgaaacttc cagttctaat ttaggattgc
    29701 gctcgatcca acgcaaaatc gtttttattt gtaatgatac ggctgttttt gaaagaaaga
    29761 aaatgtctgc tatcttttgc atactgacat accctttttg aaacaataag tacgtcacaa
    29821 tagttgaagc tcggaaatcc atgctggaat agattttatg tttcgtcacc tcttgtatga
    29881 tttgctgttt agtttcagat tcaattgatt ctaaaaaata acctgaacgt ttatttgact
    29941 ggataatgat tttcttctcg tagatagcaa gcatattttt gatttcagtt tggatcgttt
    30001 tcttgttggt acctaaagct tcagctaatt cttcgcttgt tatccaatga ttcttatttc
    30061 ttaatactac aagaatttgg agttgtcgtt tatttaagtc catttgctta cacctcctct
    30121 ttgttataaa catacaggaa aaacctctca aatgacaata gtcacttgca gaggtttaaa
    30181 aatcatttga agaaagatct atattcaagg tttttaccat tagactggat aatttgttta
    30241 taatattcaa aactatcttt tttcaatctc ctaaagcttc cgctcccatc attttgtcta
    30301 tccacataaa taaagccata tcgcttcctc atttcccctg ttcctgccga tacgatatcg
    30361 attggtcccc accataagta accaatgatt tggcaaccat cttttattgc ctcagcaatt
    30421 tcctgaagat gtatttttag atactcacaa cgttttggat cgtggattcg attcaattca
    30481 tctggtgctt catctagtcc aatgccattt tcaactatga ataatggtaa ttcataacgg
    30541 tctgtcaatt cattcatgac ataacgaaac cctagaggat caattggcca actccaaggt
    30601 tcgggagatt tttccttcaa aaagggatta tcgaaaccat ttgctccgcc agtatctacg
    30661 cgcatttctg cttctttttt atagacagcg ctacgataat aactaaatgc aatataatct
    30721 accgtgttag cttgcaagta ctgttcgtct ccatctaaca ttactggtgc acagtcttct
    30781 tccttccata tcctgtaaat ataagctgga taatgccctc ggcacatgac atcggagaaa
    30841 aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc acagtcatat
    30901 gcataagtcg ctaatgcaga aaagctacac ataataccca tcttagcatt tggatcgatt
    30961 ttctttaata attgagtagc acgggcgttc gctagaaaca tatgatgaca ggcttgataa
    31021 atctcttttt ttgttagtgt agcaatgggt tcaagtttat ctctagcagg gaatgtaacc
    31081 aatccagcag ctgcataagg catacgaaaa gcattattga tctcattaaa ggtcatccaa
    31141 tatttcactt tcccttggta gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg
    31201 attaattttc gattttgcca ccccccatac tctcctacca atgctaaggg ggtttcataa
    31261 tgactaagag taattactgg ctcgatatca tatcgattta atgtattgat tagtctgtca
    31321 taaaatacga gaccagcctc atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc
    31381 catgagatgc ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg aatatcctct
    31441 tcaaatcgat gataaaaatc aatcgcttca tgagaaagat aatggagtga ttcgtctaaa
    31501 gcaatctccc aaatcttttt ttcttcattc cactgaatac taggatgctg gttcatgatc
    31561 ccgataagcg tatctgtcac attaggtaat ttgccgtctt ccaaccatgc accttcacat
    31621 tgattagcag ctactgctcc gccccataaa aaattttctg gaaatatttc ctgatccata
    31681 actatctctc ctcgctatca acaatttctt acttgaagta tagctcgatt catcaaaaca
    31741 agatattcta actatttcgg cagaagagaa gaaaaaaatt gaatgctact cttcattttt
    31801 aatatttata tataacaaat acataagacg atccccgaaa gaaaacactc gttaaccatc
    31861 taagcaaacg atttatttta cttccaacac aaatgcttca taaggtcgta aatcaacttt
    31921 gccgtctggt aactgtttca gaggataatt tgacacaata acccttaaag cttcacgtgg
    31981 aacagtgaac gtctgctttt cactggacat attacaaata acgagccatt gctggtcttc
    32041 atatatgcga gaataggcaa agacatgctc aggcgtcttt tccactaatt catagtctcc
    32101 ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac
    32161 agaatcttga tctgctaacg cttcttctgc attgatttct ttataatttg gactgactcg
    32221 aagccatgga attccagtag taaacccagc atgttcacgg gcgttccatt gcataggcgt
    32281 cctagcattg tctcttcctt tcgcattgat tgattccaag atttcctctt ttgtaaatcc
    32341 agaagaaatc ctttcgttat acatattgat tgtctcaata tcttttgctt ccgcgatatc
    32401 atcgattggg caattcgtca tgcctaactc ttctccttga tagatataag gggtgccttt
    32461 catcatatgg agcaagatag caaacatctt ggcactttct tttcgataca catcgtcatt
    32521 tccccaacga gaaacaattc gcggaagatc gtgattattc caaaataaac tattccatcc
    32581 ttcatcacct aaagatgttt gccatttgga taatactttt ttcagttcag agatactcaa
    32641 gggtttcaaa tcccatttgt ctttgccttc ttgctgatct aatcctatgt gttcgaattg
    32701 gaagaccatg gataattcat ttcttttagg attggaataa agtttagcga tttcggtagt
    32761 tgctccccat gtttcgccaa cagtcatcag atttttgtca ccaaaagttg cttgattcat
    32821 ttcctgaaga tactcatgca gtttaggtcc attacttgtg atttctttat ccggttcttt
    32881 gccaatcata tcgatcacgt ccatacgaaa gccaccaata cctttatcga tccagaaatt
    32941 catcatttga taaatctctt ggcgaacctg tttatttttc caattcaaat caggttgacg
    33001 cttgctatac aagtgcagaa aatattgccc cgttttttca tccaactgcc aagcagaacc
    33061 actgaatatt gagcgcaatc tattaggctc ttttccattg accggatcac gccaaatata
    33121 atagtcccga taaggatttt ctttattttt acgggcctca atgaaccacg gatgttcatc
    33181 agaagtatga ttcaccacca gatccatgat gatacggata tttcttttct ttgcttcttc
    33241 aatcagttct tccatttctt ccatcgtacc gaattctgcc atgattgctt gataattact
    33301 gatatcatac ccattgtcat cattcggaga ctcgtatact ggagaaagcc aaattgcatc
    33361 aatacctaaa tacgccagat aatccaacct ggaaataatc ccccgcaaat ctccaatacc
    33421 atcttgattt gaatcttgaa aacttcttgg atagatttga taaacaactg aatggtgcca
    33481 ccatttttgt tccatgattt tcctcctata aatggatgtt tctatttttt gcttaatcat
    33541 ttattttagt ctctctcact aaaaattgta acgctttcaa atagctattg caccttgtta
    33601 tcggtaacag aataaaacaa aaaaacagct gaggaaattc cctcaactgt tttttgaaag
    33661 gattgattta ttcagggaag aatgctgatt tcacaacttc tttataaacg gtgttcactc
    33721 agctgtccct cggtattagc tcaaaaaacg gcaaaatata agaaactatt tttttcgttt
    33781 ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa cggtgttcac
    33841 tcagctgtcc cttggtatta gctcaaaaaa aggcaaaata tgaggaacta ttttttcgtt
    33901 tttttctcta atactcaggc cagaacactg atttcacaac ctctttattt cacattcaat
    33961 atccacatga atcccatgaa tacaaagaac agaccataca tgatgggaga tacttctttt
    34021 cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc aatcccatct
    34081 gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat cgcaatctct
    34141 aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac cataatcaat
    34201 gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa caagcctaac
    34261 aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac ggcgatacca
    34321 gctgatgatt ctacaaatgc accaacagga gaagtaccta ataaggaacc cgcaagcata
    34381 gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc tttcatgaaa
    34441 cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt cacaagcaag
    34501 aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg ttttaatgca
    34561 actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg cattggaatc
    34621 aaacctgtga ccagacctaa aatcgttgta gctgccatcc cgatgaagat gccacctggt
    34681 acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa ccaagtagaa
    34741 ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat gatcccacct
    34801 tcgctcagac caaggaaagc aataaataga ccgataccgc cagaaatcgc atatttcaaa
    34861 tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat caaaataaaa
    34921 atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc cattcctaaa
    34981 caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc aattggataa
    35041 cgggctaata cccccattaa aatacagccg atcgcactgg ctaatgcagt cgcagtaaac
    35101 actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa taagatataa
    35161 gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt ggtgttcaat
    35221 ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc taaatccgaa
    35281 cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg ttttatcata
    35341 tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc
    35401 atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt acaagaattt
    35461 ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt gtgatttgat
    35521 tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag gattcagatt
    35581 atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct tcttttttcc
    35641 tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat acttcaaata
    35701 accttctaat cttcccatta gatccactgt ttctcctcct ttttattcaa tcagacgact
    35761 cagcttttcc tataacaaaa taattgcctt cagggtccga aaaattgaaa gtcaagacgc
    35821 cattgttgtc tgtgatttct cctggttctt ccatgttctc gtagatagtt tcaaattcat
    35881 ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa
    35941 atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca atactcttaa
    36001 attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct acaaagaatc
    36061 ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt atcatcgttt
    36121 gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt atatcacgaa
    36181 agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt ttctaggatt
    36241 gctttcctcc acaaactttt tttacaaaaa aaaaacggca atgaatgccg ctcttcttat
    36301 ttcgattctt ttataattct gcttctgatt catctttggt tagtgtgtgt ctaacactag
    36361 ttacgccaaa tgtttgatcg attttttcct tcaacgcttc gattgcctgt gcgccacgtg
    36421 ccgcttgttc agcattgatg gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca
    36481 gcatcgttct ttgggcttca cgccagttga gacagcgaca gatcgctcct tgttcatcat
    36541 agtagatgat ttcttccgga agtgccggag catcggaatc tgcacctaaa ggcaggaatg
    36601 attcgtcacc ttgggctttt cctaaatgca gatttcctgc gatcttttcg atatcttctc
    36661 cgcccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    36721 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    36781 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    36841 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    36901 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    36961 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    37021 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    37081 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    37141 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    37201 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    37261 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    37321 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    37381 ctgtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    37441 aatcagctaa atgctccggt aattatcttt tccaaatgta tctgtaaatg tttcgatact
    37501 aatttgttgt agtaagtcta catctgaagg cgtcattttt cgaatttcgt atttattcac
    37561 ggcttcttcc tttcaataga tccttttatt gccttttttg acaaaattcc aatcttcgct
    37621 aacattcttg catactttat ttaaaagata ttccaattgt tttgcttctt cgtcagaaag
    37681 accatttaat gcgactgcat tggagtagtt gttttctcga atgatgaaag gatagatttc
    37741 tgctcctttt ttagtcggat agagtttctt gattttttta ttttctttat cttccaaacg
    37801 ttcgatcatc ccattggatt ctaatttctt gattgctcgg gcagtcgttg tacgatctac
    37861 tttgatcatt tctgctagtt tttcttggat gattccagga ttttcgcaaa tacgtactaa
    37921 ataaagatac tgtcctcttg ttaaatcgac ttctttgaat tcgatattgc taatagagtc
    37981 tagcgctcgg gcaatcattc cgatgtctcg aaggatttca gccatttcat cccaccttta
    38041 ttttgtttgt aagactagtt tacctctttt tgttgcaaat gcaactattt ttaataaaaa
    38101 aatcaagttc ttttttaata actagtaaag tatattcttt tcttctttta cgaaaaagct
    38161 caatcacaat agatctggtt tttgttttct aaaataagta actgtttctt cgttgttcgc
    38221 ttttctgata gctttggcaa ctcttctata ctaaaaaatc ccatgttgga agtttcatta
    38281 ttctcaataa acttggcctc ttcaccgtgg atcgcacaag caaaaacaag tttataatac
    38341 tgaaaaagct gtggaatatc tttttgttta ttcgtatcaa aaactgctcg caactgagta
    38401 gtttctactg ttaatcctgt ttcttcatag acttctttac gcacattttc ttcaggagac
    38461 aatcctattt cagcaaatcc accaggaagt gaccattctt tcgtccgttt atcttcgatt
    38521 agtaggacct tgccatcttt tttgatatag gcacgaacat ctacttttgg tgttggatgt
    38581 ccttctccaa tcgttgtgag cgctttcatt tcttcgaaag tttcttttcc tatttcactg
    38641 atcagttcca atgaaatcgt ccggatctcc tcaaaacgct ctttatcaaa tacatctttt
    38701 ccataatata gaccagcttg agaaagtgcg agtagtcttt tgtattgagc caacttcttg
    38761 ttcattctct tcccttcttt tttagcaaat gtatgactat gtaatcaagt atagaagact
    38821 ggtttaatta cggcaaataa atataatgaa atctgcaata aataaaagaa tgttaaaaaa
    38881 ctcgagaaca aaagacattc gacttctctg gggctgtgaa taaatggcgt tcaaaagctt
    38941 gcatctgcgg taataagccc catcaaacga aaaatatgac gagctattct tgtctggctg
    39001 ttcttattaa ttgaggcagg acagcttttt cacaacctcc ccactaaacg gtgttctcct
    39061 accaactcct cggtcacaag tcacaatatt catcaaacat gagaagctgt ttctttgaaa
    39121 ttgctccttg tgactcggag ctgaacgaca ggctcacgac ctctcggtaa acggtgttca
    39181 aaagccaatt tttcgatatt aagccatttc tttgttactt attgtcattg atttcttttt
    39241 ctaatttatc caatagtgga gtgtaatcca cttgagtata gtctgttaca tcaaattcat
    39301 atagtgttcc taactggaat ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt
    39361 ctttcgtaat atgattggtc cctttcgttc gatcttccaa tacgtcacca tagtgataat
    39421 gatccatgat atagctcaaa tgacgatctg attcacgatc acgtttgtac cgacgttgcc
    39481 ataacacttc aaagtctgct gttaggcgaa tcgtgatcac ctgatatccg tatctatctg
    39541 ctaattcttg gagtcgtgct ttttgcttat cactaaaagg atactcggac acaatcactt
    39601 tctttcctgc ttccatatac aaatcaagtg ctttgtaata gaaaggataa actttcgtcc
    39661 tttcttgttc cgcccgttct tccaaattgt taaacccgat ggattctgca tagtattcct
    39721 tgatttcatc gagcgtgatg gcatacatat ctggaaatcg ttcccgtagt ttattcataa
    39781 gatatgtttt tcctgtacca ggactaccag ccagaagaat gaaatacttt ttcatctcat
    39841 ttatttccta agttaattgg actttccaag ttcataaaca gtccaatctt atgcttgaca
    39901 atttctttta ctgctttatt tgctggaatc acattatggc gaagagactt gttgatttct
    39961 gtttcactaa aggtttcttt agctactttc gtccagttga ctaaaagctc cgttccaaca
    40021 ttgaatttgc ggatcccgtt attgatcaat tcaggataat cttcttcttt cacacctgtt
    40081 cctccatgga taactagggg gacatcaact actgcatcga tttctttcag caagggaatg
    40141 tttacttctg tttttgattt gaattgtcca tgattcgtac cgatagcaat agctaatgca
    40201 tccacgcctg tagcttctac aaatttcact gcgtcttccg gtcttgtata taccttgtca
    40261 ttttcagcga catggattcc ttcttctgta ccgccgattg ttccaagttc tccttctacg
    40321 gaaacaccgt gcgcatgggc atattctact actgcttttg tcttcaagat attttctttg
    40381 aaaggtagat gggacccgtc atacatgaca gaagtatatc cgctatcgat tgcttctttg
    40441 atatcatcaa agtctcgggc gtggtctaag tgtaagactg catcaaccat ttcttcttct
    40501 gcaatagctg tcattacatt gactaacaca tgcatgccga tatatttagc ggtatcgaca
    40561 cttgtttgaa taatgattgg cgaacccatt tcttttgctg cgcctagcat ggcaggaagc
    40621 atttctaggt tatgcgtatt aaaagctcca actgtaaaat tcaactcttc tgctgttttt
    40681 gttacttctt tcaatgttgt atacatcttt tattgccacc ttatcttttt atttgtttcc
    40741 tttgatcgtt acttgttttg caatttgtcg catctcatcc attttgccca tgtagtaatc
    40801 gatcccttga tcatcaccag cacgagcagc atctgcacgc ttctggttat agaggctcat
    40861 cagtttttta tacccatctc cgatcgtctg tttattttcc aagctctttt ctaaatattg
    40921 gatggcttga tcgttctcat gcaattcaga aaatttaagt ccaacttgtt cataaagttc
    40981 cgctaattta tgcctatctt gggtagtgga aatctcttgt ttcaattgtg taatctctga
    41041 ttgaatcttt tcggtcgctt ctggcgatag ggctttttct tcttttgcca gaactttttt
    41101 cggttctttt tcttttttct taaaaaatcc aaacatcatt ttccctactt tcaattaaaa
    41161 cgctttcaaa actgggctta agatccaggc atataagagt gctgcggcta ctgtatctac
    41221 atcggttgct gtagcattga tgaatcccat cgaattaaag atcgttacta gcaaagcggg
    41281 caataatgta ataaagaaac catgagcaat accgccaatg atcgctcctc ggcgtccgcc
    41341 aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag aaattggtta gcataccagg
    41401 taaaatcatc gctaagccga acatgggcaa agtaaacatg gcaataatcg taccaatcgt
    41461 tgtcgtaatg aaacctaaaa tgacggcatt tgggctataa ggaaagaaaa ctggacaatc
    41521 taaggcaggg atcgcatcag gaactaattt catagctatc cctcgaaatg ccggcacgat
    41581 ttcacctaac agtagacgta caccagctag caatatatac acacccacta cgaattggat
    41641 ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct gataattcag aagcgaatgg
    41701 ttcgcctgca aataaaacag tgatgatata tagcgggacc atcacgacca tgacagacaa
    41761 ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga tcgacttttt
    41821 attttcgcct ttctcaccaa aaagcttagc aacaccggct tcaaataagt acccgatcgt
    41881 acaaaaatga cctaaagcaa tatcatttga tccagtgatt ttacgaatga tcggttgcgc
    41941 tactgccggc atagcaatgg cgaaacaagc tccgataaaa ccaccgacca agatcaatac
    42001 gatccctcgc agaccggcaa aatagccaaa gactgtcgtc atcgttgcca tccataaaat
    42061 tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa
    42121 tacaaaaatg gctaagaacg tcagtgcaat atctctacct aaacccaaat cattcattgc
    42181 ttgatcattg atagattcaa tagaagggat gatcccttgc atatgaaatc cttcagtaaa
    42241 gattttcccg aaataagtta aacttccaac gatgatgctc gaaccggcac tcaatacttg
    42301 aaatcctagg agtgttttta acgtgccaga gatgacttgt cctgctgatt tcttctgcag
    42361 tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca aaatattttc
    42421 gatgataaaa ttgataacgc tcatgttttt accctctttt ctttttgtat ttagataact
    42481 cccttatctt gcagaactgg ggtgattttt tcttcaattt cattttttga aacaatattt
    42541 ttcaaataga tcatcgccgt tttaggatca actgcgaact tttcgaactg cgaacgaaaa
    42601 ttttctgctg tgatgatcac atctggtttc atggaaactg cggaagaaat atcacaatgg
    42661 tccagtttgg cttctactcc taatttgttc aaaacatcct cagcagacat ctgtgctgca
    42721 aaactacttc ctaaacctgc gccgcatacg aataaaatgt tcaatttgtt catctttgtt
    42781 ctcccccgta tcatttatta tttctcaact actgaaaaaa gcagttgttt gaaatcttcg
    42841 atatttgtgc attcaatcaa tcgatccaac ttctcttcgt tgttgatcaa ttcaattagt
    42901 tctttcatga tgttcagatg ggagtaggag tctactgctg ccaaacaaaa aataatcttt
    42961 actggatcca tttcaggatt accgaaatcg attggttgct caatcgtagc tacgctgact
    43021 cctaatttat tgaccccatc ttctggtcgg gcatgggcta gtgccatgtg tttgccaatc
    43081 acgatatacg gtccgtaatc attcactgca cgaatcatcg cttcgacata gcgctcttcg
    43141 acaatgtttt ctttaaccaa tggtctagca gctagttcaa tcgattcctc ccaagtagtg
    43201 gctttttctt tgatcatgat attgctgtcc gtcaaaatat cttttaacat aggctggatc
    43261 tcccttttgt tgatttctaa attgtttgtt tcgaataatt tttctaactt gccatagatg
    43321 attcgatcta ctcggccacc actttctttc atgacatcta ttatttgagt gaataactcc
    43381 gtcgattcat gactgttagg ctctaaccgc tgtagttctt catttctttt caaaaagtct
    43441 tctataacca agatattcgt ctcagttaaa atcggatcga caactaaaag cggcttattt
    43501 cgataattca attgaaaggt gctgaaaacc agattgacat ccaatttatc tactaattcg
    43561 atttcttttg aacttaaaac tgcagtaatc tcgatctgag gaaacttttc tttcagattc
    43621 tctgctaaca gatttccagt agccatccca tggttacaga caacgactga tttataaaca
    43681 taattcaagt ctcgcttgat tacactggcg atcgtcgaaa agtggatgac aagaaaagct
    43741 atctcgtcct ccagtatctt gccaccaatt acttcttcga tcgtaggaac aaaatgagtg
    43801 actgcgcggt agattgggaa ataatttgtt cggatgtttt ctttcaatgg attcatgact
    43861 tggatatgat tcttgattct tacaatcagt gcagccatgt gcttatagag attttcgaat
    43921 aaggcttctt cctttagatg gaaaggtacg tgtgtctcgt tctctacaaa ttggatcaat
    43981 tggatcgtta gtaattgagc ttgtacccac tcgattgaat tggagatatc tctagcattg
    44041 aacgtctcaa tagtgaaacg aagataattt ctttctactt gcgggacatt gtccaattga
    44101 aattcctcga tcatgtggtc aataaatgca gaaaagctac tgtcttttat ttctgtattt
    44161 ttccaattaa tggcagagat aaattcatgt ttcctgattc gctggatcca gatcaatgta
    44221 aaaagcagga tttgtttttt ataaatatca tcttcttgtt tcaacaactt attggaatag
    44281 atctcctgta tttttttgat ttcctccaaa ggaaaataat ggagaaagat tttttgagcc
    44341 ggtgttaatt gtgcatcttg attcataaag aagtcggtca ttcccagatt ctgattgata
    44401 aaatcataga tcatcgtacg gatagatcgt tctgaacctt tataaaccag tccttgcttt
    44461 ccataactga cgatctctat cccataactg accagtgttg ctcgtaaccg tctcatatct
    44521 tcatctaatg tacttttaga gatttgaaat tcttcttctt ttttgtttag atggatcggg
    44581 attcggctgt aagcaacggc taaaactaga tcaaaaatac gttcgcgccg attcaaaaag
    44641 ccgtctttca catcttttat ttcagtttct aatcgagctt gcttttcctt ttccagcttc
    44701 aacaaaaaac ctttgttgcg aattgtacgg atctccggaa gctttttact gctcaaccag
    44761 atattcgctt caagaacttc ctgacgaatc gtccgctgac tgacattgaa ctcacttgct
    44821 agatgttttg cttttactgg atattgggct gtcatgattc tgtgaagtat ttcaattgtt
    44881 cttgttttca tagaaatgat ctccttctga tttcaagtat atggttccgc ttacataata
    44941 aaaagtagat atttctgcca cttgaagtgg aaagaaagta agcaaagcaa acaaaaaata
    45001 agccatatgc tttttcatct cctcaacgaa aaagacatac gcctattatt acaaaaattt
    45061 tgcccatcta tacaaaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    45121 actaaactaa aatagtgctt cctatttttt gtttggttgt tcttattatc gcagatgcaa
    45181 gcttttaaac accatttagt ggagaagctg tgacagagat gttcagcctt gagcgataag
    45241 aagcgatttt gaaaaatcgc ttcccatatt ttacaaaatc gtgccttatt gccgataggc
    45301 tatctctgga acaccgttta ttcagaaaaa ggagctgcga caaggctttt gtcacaactc
    45361 cgttcataaa tatttcctgt caatccagca taatcggttg aataaaatcg gcttgccatc
    45421 ttttttcttt ctcagaaaaa tcaaaatacc atatctgaga aaaatactgt cgttgtgtct
    45481 tacttcctga gatcaatcgc ccatcttttc gatcgactga ataatcacag catctgaaat
    45541 caattcggat ggcaaaacta ttttcgtgga tctgtcgata atttcctact tcctgaatag
    45601 ttattttttt agtatgattt cttacaccgt ctgctttgtt tttttgcaga actttctgat
    45661 gttcggcaaa cagcttgtct gtataacagc ttctggcttt ttcaggagtt tcttcttccc
    45721 aagcggattg gatcttcatg aatatctttt ggatttccaa aaactgtgct ttcttttctt
    45781 tagccgttcc tgacatgcgg gacaatatct ccgatttcag ccttttcttt tgtcttttat
    45841 tcttgacgat agaaaaagca gaaacccctc ctactgacaa aacagctaat gcaagaaacc
    45901 ccgtttgctc ataactagaa ggacctgtac ttggaccata atacccataa gaatctctgc
    45961 cgtaagaacg actgccaaaa taactgcccc catatgaacg accgccaaat gaacctctgc
    46021 ccgaacttcg accgctagat gtacctccac catgaccacc cgcgacagcg aatccttggg
    46081 tcggcagagc aaacaaacat aggcccacta acaaaatgat tgctaaataa ttgatttttt
    46141 cattgtcttc ctcgttatct tttattacaa aaaactattt ttatcatagc cttgctaaaa
    46201 ggaagagtca ataaccagat gcataaactg tttttttagc aattttgtct tatttagtca
    46261 ttgagtaaca tcggacagat gtatgtgtcg gcagtccata attcctctat cttcagcatg
    46321 aacaagcttg cagacaagag atctgcgctt cctccaggag agatttggtt ttcctgacaa
    46381 aagtgcaaca gtgctgtaaa tggacgattt ggtaaagcac tttgactagc agcatgagca
    46441 agtttttgca tttccactaa cgttttctga tcactgcgat gcaatacgca tgtgtcatct
    46501 agcgaactca tcaaaactaa caacatatgc atttgtttct gtacatatga atcattatct
    46561 agctccttag ctgcttggat tgcaagcctg atatgcggat accctaatat cgcttcttca
    46621 tatgcaccat ttacgccata tttttgcttc accctatgac catgagtggt ttttttatga
    46681 cgatattttg tatcaggaaa agcagcaagc ttgctgacat catcaagtat tttcgataaa
    46741 gaaaaaggct gttccatact gattttttgt gcaatgacac tgatcaatag ccccattgcc
    46801 caaatcgctc ctttatgggt attgatgcca tttgtagcaa tcagcattgc cttttccgct
    46861 tttcgcccaa tctcagcaat tctttctctt aactcctgat caatagcata atgccatgct
    46921 atttgagcca tttcttcaaa aaaaggagtt agcgtcttag cagacttatc caaaatgcgc
    46981 cgaccttctt ttacggcttt tgcgatcgta tggaaattat catctcctaa aaccatatca
    47041 gctgcctgtt tgcttacttc actacctttg attcccatag cgattcctat atcggctctt
    47101 ttcaaagcag gtgcatcatt tacgccgtct ccggtcattc cgacgatttc tccatttttc
    47161 tgcaaagctt tcacgattcg cagtttgtgt tctggtgtcg ttcgagcaaa gacgtcgact
    47221 tttggtactt gtcgaatcaa ctcttcttca gacataagat ccaaatcgat cccttctaaa
    47281 actttagcag tatgttttaa gccaatttgt tccgcgatcg cttttgctgt atctttgtga
    47341 tcccccgtaa tcattttcac tgagattcct gcttgttggc attcctttac agctttgact
    47401 gcactttctt ttggcggatc aatcaaccca gcaatccctg caaaagttaa ttgggttaaa
    47461 gactcatgcg tgagttcctg tttcgaatct acttttttgt aagcaaatcc taaaacacgc
    47521 tgaccttttt tcgcaaacgc tgtggctgtt tgaccccatg cttcttattc tgaagaagaa
    47581 agagttgatt tagccaataa tacttctggc gcacctttga cgtaaatcac ccctcctttt
    47641 tcctccgcat gcatcgttgc catatatttg tattcagagc tgaacggaat ttttgcttct
    47701 atttttttca aaggcagatc ggctgtttcg gtgtggcgaa tcagtgcaag ttctgtcgga
    47761 ttgccactta aattttctgt tttctgttct tctttcgtct ttaattcctg acaatttttc
    47821 ataataaaca gtacgcgagt ttcttccttt gctgctacat ccacaactgt catttcattt
    47881 tttgtcaatg tccctgtctt atctgaacag atcactgtca tagaacctaa tgtttctaca
    47941 gaaggcagtc ctttgatgat cgccttttct tttgccattt catgtactcc gacagatagg
    48001 atcatggtca acacagctgg caatccttcg ggtaccattg acaccaccaa agcgatcata
    48061 gctgaaagaa ggatatgcca atccatgcca tagcggaatg ttgtaaaaac aactaagaac
    48121 aagatcaaac aaagaatgcc ttgaaaaatt tgtttgttca atcggttgat cttcttgatc
    48181 agaggcgtcg tttgttgttc gaccgattgc agtgcttgat tgattttacc gatttctgtc
    48241 gtgtttcctg tttctacaac gacaccgact gcagaaccag attgtacaag cgttcctgaa
    48301 aaagccatat ttttttggtc accgattgat tcttctgctg ccaaaacgga atgatttttt
    48361 tcagatgctt ctgattctcc cgtcaaaatc gattcttcta ctagtagatc atgtacttcg
    48421 atcaaccgaa catctgctgg aacgacgtct ccggcacgta agacaatgac atctcctttt
    48481 actagttctt tcgcagggat tttttcttgc atcccgttcg atagtactat cgcttcttgt
    48541 cccatcagct gcttcaaacc attcaacgat tcttctgctt ttctttcttg ccaataacta
    48601 acgagtccgt tgacgatcac tacgaataaa ataatacttc cttcgatgta ttcgctcgaa
    48661 gcaaatttca aaaaagaggc aatgatcaac acgatcatca gcagatcagt aaagtgtttt
    48721 gctatttttt tccatcgttt tgtctgctgt cttgcttcta tctcattata tccttgatca
    48781 gtcaggcgtt tctgccgttc ttcttgcgac aagccttctg ttgatgtttg atagtttttc
    48841 aatacatctt ctaccgtcaa tctgtaccag ttcatcctat cccctcctgt ttaattgata
    48901 ctgttattat acgcagggaa gttttttcag gcgtatcgat tatttcaggg gggtctgaaa
    48961 taattcgttt tgcagaatgt atctgggcct aactgatatt tctattgctt attcttttat
    49021 atacaaaatt taagaataca aaccagataa ccaataaaaa aggaataaag cgtgtcgtgc
    49081 tttcgaataa caagctgaaa gcaattgaag taaaaaacac caacaaagca agatctttat
    49141 atctttgtgt agtgttccgt ttcttttctt taataaattg aacatgtgca cacaagatcg
    49201 aagaccacac aaatagaaaa caactcgttg tcatgcctgc caaaagctga aaaaacaatc
    49261 gactatcagc tacaaaaaaa tgtagacaaa gaccgacaaa aaataacatc cctgtaatta
    49321 aaataccgtg taaaggtacg cctttcttag aaagcgcaca aaaccaatgg ggcaaacttt
    49381 tctcttttgc tgtttgatat aaaatcctgc tagtactgta gactgcacta tttgctgagg
    49441 acaaagaagc aaccaaaatc attagattga caattgctgc tccgctttgg caatggatca
    49501 cttgcagcat agtcactaaa ggactttctt ttaaatctaa acaatgccat ggaacaacaa
    49561 ataaaatcga caaaatcgtt cctatataga aaaagaggat acgccaaggc agacagttga
    49621 ttgcattttt caaattcttt tgtgggtcac tcgtctcacc aactgttaat ccgatcattt
    49681 caattccagc aaaactgaat aaaaccatag gaaatgaagc aatcattcca gcaaagccaa
    49741 acgggactaa ttcattgagc tgtccaaaat ttgctttgat tccaatagac tgggtatgaa
    49801 aatctgagac actcaatcct atacccaata agataaaaag gaaaatcccc aagatcttga
    49861 tgattgagaa aaaagattcc aattcaccaa accacttgac tgcaacaagg ttgactgcca
    49921 ttaagatcat tagtgcgcta cctccgctta tcaaagctgg tacatgtaca aaccagaaat
    49981 ggatatacaa tcctattgct gttaattccg caagtccagc tccaagccag caaagccaat
    50041 aagtccaatt gacaaaaaat gcccactttt tgcctaaata atagtcaacg aaatcactga
    50101 tagagttaaa tgtactattt gctaaaaata aatcacccat gacccgcatc aaaccataaa
    50161 tagccaaacc gcatatcaaa taaacgagaa taacagctgg ccctgctgat gcaattgcct
    50221 ttgctgaacc taaaaataat cctgttccaa ttgcgccacc taatgcgatt aactggatat
    50281 ttcgattagt tagagtccgt ttcattgccg gtttttccat caagttcacc tttttctttt
    50341 tcaagcagca tggcgacttc ctgttcccaa gaaggtttct ttattttttc aatacagagt
    50401 ggtactacaa acaaaatgac actgatggct gtgactgcaa ttagattgat ccatgtccca
    50461 gctttcatcg tcagtccgac agaaaatccg attgccgcaa ataaaacaat aacgacagtc
    50521 caaactccaa gattgctctt tttctctttt tttccaattc taaatggtcg attcaacttt
    50581 gcatgtgtat aacgcaatct caaaactgcg atccccatga tgacatagac gatacagtag
    50641 ataacagtag ttgaatttac gagcatcaaa aatgcttgat tgacgccagg aatcagcaaa
    50701 taaaaaagtg caaagattga gataatgaca gactgagtca aaataacgga tttggatacg
    50761 tcaaattgat tcgtcttcca aaaattgaat tttggtggat acgcaccttt tctagcactt
    50821 tctgtcactg tctttgctgg tcctgaggcc caagcagata attggactgc tacaccaatg
    50881 aagacaagta aactaaacag attcacaata agatgtggca aaccaagaat ttggcaataa
    50941 atcgaaatag attgcgcgat attattcaat tccatttgtc cttttggtac gacatttgct
    51001 acaactaaag cattcaaaat attgacgaaa aatataaaaa ttaacgcggc aaatactccg
    51061 cgaatatacg ttttaactgg atttttcaac cgagtgatat acacagagga catttcgata
    51121 ccagtaaaaa taaacatgat cggcgcaaaa taaacaaatg aggatgcagt cgtagtatct
    51181 ggtattaatt tgtcccatga aaaactgcct aatgttccgg ttggtacgat cccaactttg
    51241 atccacgcag caaaccctaa taagagcatc atcgtcaaag gcacgtacaa tcctaaccaa
    51301 acaccgactt ttccgccgat ttttgccata tcaaatttca aattcaaaaa agtgatgatc
    51361 caataaacga ctaaaatgac aactaatgta aatttgctgt tcaaaccaag cggcacatta
    51421 tcgataatat taccaaacaa cggagcaagt gctgaagcga ccatcaccat cccaggaaac
    51481 atttggaccc ataacagcca tgagacaacg aacccccatt tttttcctag agcattcgat
    51541 acccaaagtt ctggaccacc tttttgggga aacattccgg caaattcacc agagatcaat
    51601 gagattggaa aagcgtacaa taatactgca accagcatgt aaaaaatcat cgtccagctc
    51661 gttgctgcta catctggaat atttcgtaca cttgctacaa gggcgcaggt cattccgata
    51721 aaggtcatgg tcgtgatttg ctttgatgaa ccagtatctt tcatctttat tccttctttc
    51781 aatagcgaca tatgtttttg ttttctttta tttttgttca agaacagaaa tgacaaggtt
    51841 tcatcgattc agtacaaaac ccagtatcac cgaaacatcc attgaacgat cttagtgaac
    51901 tgggtttgat acatgaaacg aacgatccaa cttatagaac gttttataaa aaagaatatc
    51961 ttggcgagga atatatgaaa aacaggggga aatacaattg ttttgatgaa gcaaataatt
    52021 gtctgttctc catatgtctc ttgttattct ttcacacaaa aatcagttct attggatgat
    52081 ttcgtttagt ttcttttcca ttgctttggc gatttctttt gtataaaaat tgaaaattcg
    52141 atcatcattc aaataaggag tcatgatagc cgcacgtagc agagtgattt tctgttcttt
    52201 tttccattct tctttcgtta gacccattct ttcaacaaaa cgaatcggag aatcaccgta
    52261 atcttcttgt gtaaatgtag tatgagaagt aataaaccgt tcaccataaa catcgccatc
    52321 catataagaa gaccgatcga acattttttc atttaattca ttgatagcgt tcaaatctgt
    52381 acagccttgt tctttgaata cccaattgac catattaaaa tcaggatgat tcaatggata
    52441 gacttcgatt gtttttcctt taacagtaaa agtcagatga tcgagaaatt ctctgaatct
    52501 ttgagctgcc tcgattgaag ccccaatcaa ttgaccatat ccggtcacat tcaatggcaa
    52561 tacacgatgt gctgtccaga ctgcggcggc ggttgctcca gcttttgatc cttccaaaat
    52621 ataagctcct aacatatctg gagcctttac tgatttttca aacacatatg gcgcgaaata
    52681 agaaatgata tttctcatat ttttatgttt gattacgatt cctccagcag cataagggac
    52741 atagcccatc ttatgaggat ctatcgtgac agaatccgct tctgaaatag ctctgaaccc
    52801 ttcatatacc tctttgtcaa tcgttacgca gtggtggaaa acatgatgtt cttcaaagaa
    52861 ttcagctaag gaagcatacg gcacaaattc tcctgcttcg tttagaaaca gtgaacgcgc
    52921 atagccgcca tatgcggcat caacatgtaa atagaaatag atcccttcat cttttaacct
    52981 ttctcttaat tggacgattt tatctacact gtctacttga ccttcttctg tcgttcctac
    53041 gacagcaacc acacctagaa ttgggatttt ttgttcagct aattcacgaa ttgttttttc
    53101 caaagcatta atatccattc gataatcctc ttgaactggg atagcaacca tctgatctaa
    53161 gcctactccg caaatatcta acgccttcat ccaagaatag tgttttgttt gaggcaccag
    53221 ccatttccct aatctttgga tattttttcc gctacgggaa gaagctgctt tgacctcatc
    53281 catttcttcg tccgtaaagc tttcggtcat ttccaaaatt tcttcaacag ataaattcaa
    53341 aagttcccac tcagacattt ttttcacttg ttcaggcagc acttccttga cagctaaagg
    53401 aatcgatttg atgcagcggg cgtaccataa tccttcaaga ttagcaatag aaccatctgc
    53461 tgcaatgtgt ccccatccgt ctgtcatatt gaataatgaa gcaaaatcct gtcctacttc
    53521 tgcttccatt tgcgaagttg ccatagaaga ttccaatgca acattatttg ggttccacag
    53581 catcgcatag ttgtaagcta ataaagcagg catcaatgtt tcagcattca tttgtcccca
    53641 gtaacggcct gctgaatgcc atgggatcga tcctgcgcgt aatcgctgac tgacttcatc
    53701 caagacctct atcatatggt cgcgggtggc tagataatct ggagtgagct tatctccttc
    53761 gctaattgca ggcatatctg aaggaatata attttctctc catccaagat gttcatctac
    53821 catcttgttc aacaacattt tataaactgg tccattttca gctttgtcgc ctataaagac
    53881 ggccttgata tccatatctt tcatttttct tccctccata ttttgttggt caatgtctcg
    53941 cagatacatg tgtttatctg taacacctct tcactatagc gccaaaaatg agaaattttt
    54001 ttttataaca tcaaatccac gtagattttt ac
//
LOCUS       DACCWK010000006        51045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCWK010000006 DACCWK010000000
VERSION     DACCWK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.006"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            127..972
                     /locus_tag="IVW65_000329"
     CDS             127..972
                     /locus_tag="IVW65_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7899257.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1253..2473
                     /locus_tag="IVW65_000330"
     CDS             1253..2473
                     /locus_tag="IVW65_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7899258.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2470..5190
                     /locus_tag="IVW65_000331"
     CDS             2470..5190
                     /locus_tag="IVW65_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7899259.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5174..6118
                     /locus_tag="IVW65_000332"
     CDS             5174..6118
                     /locus_tag="IVW65_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7899260.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6187..7197)
                     /locus_tag="IVW65_000333"
     CDS             complement(6187..7197)
                     /locus_tag="IVW65_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7899261.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7377..7721)
                     /locus_tag="IVW65_000334"
     CDS             complement(7377..7721)
                     /locus_tag="IVW65_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7899262.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7714..8148)
                     /locus_tag="IVW65_000335"
     CDS             complement(7714..8148)
                     /locus_tag="IVW65_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7899263.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8286..9023
                     /locus_tag="IVW65_000336"
     CDS             8286..9023
                     /locus_tag="IVW65_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899264.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9016..10227
                     /locus_tag="IVW65_000337"
     CDS             9016..10227
                     /locus_tag="IVW65_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899265.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10434..11213
                     /locus_tag="IVW65_000338"
     CDS             10434..11213
                     /locus_tag="IVW65_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7899266.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11283..11945
                     /gene="trmB"
                     /locus_tag="IVW65_000339"
     CDS             11283..11945
                     /gene="trmB"
                     /locus_tag="IVW65_000339"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7899267.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12043..13797)
                     /gene="ade"
                     /locus_tag="IVW65_000340"
     CDS             complement(12043..13797)
                     /gene="ade"
                     /locus_tag="IVW65_000340"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7899268.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13929..14858
                     /gene="cpaF"
                     /locus_tag="IVW65_000341"
     CDS             13929..14858
                     /gene="cpaF"
                     /locus_tag="IVW65_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7899269.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14815..15864
                     /locus_tag="IVW65_000342"
     CDS             14815..15864
                     /locus_tag="IVW65_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7899270.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15861..16139
                     /locus_tag="IVW65_000343"
     CDS             15861..16139
                     /locus_tag="IVW65_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7899271.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16114..16584
                     /locus_tag="IVW65_000344"
     CDS             16114..16584
                     /locus_tag="IVW65_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7899272.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16601..16903
                     /locus_tag="IVW65_000345"
     CDS             16601..16903
                     /locus_tag="IVW65_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899273.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16920..17291
                     /locus_tag="IVW65_000346"
     CDS             16920..17291
                     /locus_tag="IVW65_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7899274.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17263..17622
                     /locus_tag="IVW65_000347"
     CDS             17263..17622
                     /locus_tag="IVW65_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899275.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17794..18801
                     /locus_tag="IVW65_000348"
     CDS             17794..18801
                     /locus_tag="IVW65_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7899276.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18826..20010
                     /locus_tag="IVW65_000349"
     CDS             18826..20010
                     /locus_tag="IVW65_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7899277.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20447..22030)
                     /locus_tag="IVW65_000350"
     CDS             complement(20447..22030)
                     /locus_tag="IVW65_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7899278.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22608..22751)
                     /locus_tag="IVW65_000351"
     CDS             complement(22608..22751)
                     /locus_tag="IVW65_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7899279.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22974..23453)
                     /locus_tag="IVW65_000352"
     CDS             complement(22974..23453)
                     /locus_tag="IVW65_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7899280.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23526..23810)
                     /locus_tag="IVW65_000353"
     CDS             complement(23526..23810)
                     /locus_tag="IVW65_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899281.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23888..24082)
                     /gene="ssrS"
                     /locus_tag="IVW65_000354"
     ncRNA           complement(23888..24082)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVW65_000354"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24154..25440)
                     /locus_tag="IVW65_000355"
     CDS             complement(24154..25440)
                     /locus_tag="IVW65_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7899282.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25578..26249
                     /locus_tag="IVW65_000356"
     CDS             25578..26249
                     /locus_tag="IVW65_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7899283.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26215..26703
                     /locus_tag="IVW65_000357"
     CDS             26215..26703
                     /locus_tag="IVW65_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7899284.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26716..27663
                     /gene="prmA"
                     /locus_tag="IVW65_000358"
     CDS             26716..27663
                     /gene="prmA"
                     /locus_tag="IVW65_000358"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7899285.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27664..28428
                     /locus_tag="IVW65_000359"
     CDS             27664..28428
                     /locus_tag="IVW65_000359"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7899286.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28661..30874
                     /locus_tag="IVW65_000360"
     CDS             28661..30874
                     /locus_tag="IVW65_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7899287.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30885..31331
                     /locus_tag="IVW65_000361"
     CDS             30885..31331
                     /locus_tag="IVW65_000361"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7899288.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31502..32761
                     /locus_tag="IVW65_000362"
     CDS             31502..32761
                     /locus_tag="IVW65_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7899289.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32850..33797)
                     /locus_tag="IVW65_000363"
     CDS             complement(32850..33797)
                     /locus_tag="IVW65_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7899290.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34191..35483
                     /locus_tag="IVW65_000364"
     CDS             34191..35483
                     /locus_tag="IVW65_000364"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7899291.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35498..37267
                     /gene="aspS"
                     /locus_tag="IVW65_000365"
     CDS             35498..37267
                     /gene="aspS"
                     /locus_tag="IVW65_000365"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7899292.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37376..38251
                     /locus_tag="IVW65_000366"
     CDS             37376..38251
                     /locus_tag="IVW65_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7899293.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38517..40400
                     /locus_tag="IVW65_000367"
     CDS             38517..40400
                     /locus_tag="IVW65_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7899294.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40475..40759)
                     /locus_tag="IVW65_000368"
     CDS             complement(40475..40759)
                     /locus_tag="IVW65_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7899295.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40749..41483)
                     /locus_tag="IVW65_000369"
     CDS             complement(40749..41483)
                     /locus_tag="IVW65_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7899296.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41612..42235
                     /locus_tag="IVW65_000370"
     CDS             41612..42235
                     /locus_tag="IVW65_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7899297.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42427..43548
                     /locus_tag="IVW65_000371"
     CDS             42427..43548
                     /locus_tag="IVW65_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7899298.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43555..46683
                     /locus_tag="IVW65_000372"
     CDS             43555..46683
                     /locus_tag="IVW65_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7899299.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46702..49896
                     /locus_tag="IVW65_000373"
     CDS             46702..49896
                     /locus_tag="IVW65_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7899300.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50042..50749)
                     /locus_tag="IVW65_000374"
     CDS             complement(50042..50749)
                     /locus_tag="IVW65_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7899301.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16698 a         8932 c        10612 g        14803 t
ORIGIN      
        1 ataaatgtcc tttcctattc ttaaaatgat gtttgtactt acttaaatta ggtataatta
       61 gtatatgttt tgtgagaaat ttagacttac aataagcagt gaatttataa tcaacgtata
      121 ggagacatgg atgaaatgaa actaattggt atagatttag acggtactct tttgaattct
      181 gaccagagca ttagtcaaaa aaatgcaaga acaatgaaac atttaccgga agactgtttt
      241 ccattcattt gttctggaag agaagtagaa gacattaata atattctcaa aaaatctggt
      301 ttaaggatac cagcagtagg gttaaatggt tctgttggat ttgatggaga aaaaaagata
      361 tttgagtctt ccttcgataa aaatgatgta aaaaaaattg attctgtcct atcgaaattt
      421 ccaaccaaaa tatatacaaa tctcggaagt tatgaatctc agaattataa aaataaaatg
      481 aaaaaaattt ttcacgaggt agggagtgaa ttttctatca aagagctaaa ctatgagctt
      541 gagtatgaaa aatctatttg ttcaatccct tataataata ttgaggaaat tatagaaaag
      601 gatgctatca gcatttacaa attttttatt tttattccta atcgtcaatt gaaagctaat
      661 atatttaacc atttgaaaaa gatgctaaat gtgtctgtta ctgaatcggc acctgtaaat
      721 attgaaatcg tacctcaaaa tgtatctaaa ggtgtggtgt ttgatcgttt ggaatcaatc
      781 tacaatttaa agaaaccttt gagaatcgcg atcggagata gtttaaatga cttatctatg
      841 tttgaaagtg cagatattag ttttgcaatg ggaaatagtc atcaagaaat catgaaaaaa
      901 gctgattatg tcactgtttc aaatgataaa gacggagtat ctcaagcatt tgaaagaatt
      961 cttaccatat aaactaatta aggtagtata gaacatagaa ttctaaatac ggaaaaatgg
     1021 agcataaatt ccagtacaaa tagtacttct tcagtgtcaa acgaagcgaa tataacatag
     1081 atatttagga ttgtgacaaa aatcatgtca caatcttttt ttcgaataaa cttatttttt
     1141 gaagtcggcc atttatcgta atttctttgc ataatacgaa caaattttcg gttattgatg
     1201 aaaaaaaaca ttgaaaatgg tatgataaaa gaaaaaaagg aggaaatttt taatggtgaa
     1261 atttatccat gcggcagatt tacatatgga tcggtcattt gaaggactga caactcttga
     1321 taaaactgta caggaaaaac tattgaagac gaatctgacc gttttatcaa atatcattga
     1381 acaggcaata ataaataacg ttgattttgt tttacttgca ggagataatt ttcatcaaaa
     1441 tcgtccttca ttaaaaatac agaaacattt ttcagaacag atgaaacggt tagaaaagaa
     1501 ccatatacct gtatttataa tattcggaaa tcatgatttc tatcaaaaag agcggtattg
     1561 gttctccttt cctaaaaatg tccatttatt tacatccgaa acggtagaga caaaaaaaat
     1621 aacgataaaa agtggagaaa ctgtctctct cagtggattt agttatcgtc aaccgtggat
     1681 tcaaaaagac aaagtgatgg aatttccttc tagagaactc acagattacc atattgggtt
     1741 gtaccacggg gaacctggcg tctctcaaaa aggaaactat gctcctttcc aaccctcaaa
     1801 gatgcaagaa aagggatacg attattgggc tttagggcat atccatgtgc caacggtctt
     1861 aaatgaaaag caaacaattg tttatccagg tgcgcctcaa ggtcatacta aaaaggaaca
     1921 agcttctaca tctattctac tagttgaatt aagtaaagga agttgccaaa tacatccgat
     1981 taaagtagca gaggtctatt ggaagacaaa ggaaatatca ttgaggaaag caagaacgac
     2041 aaaagaggtc attgttcaca ttcgtcagca gctttcaaaa gtagaggatg gatttagttt
     2101 aattgagata aaattaaaag attatgacca tctcaataca gatgttttgg aacggataca
     2161 atcaggagag ctattagact atttgttaga ggaattctca gataggataa atgatttctt
     2221 tatttggcgt atttctctca tagagaatac tttttatacg aagaccccac ttgctgcttc
     2281 tgctaaattg atggaacagt tatttcaata ctatcagaca cctcaagatt tccaacaaat
     2341 tttaaatgaa gtatatagtc atcaagaagc ggctagaata ttgagcgaac tgccggaata
     2401 tcaagaagaa acacttgaaa aagccaagca gctgcttaat caagattttt tatttgagga
     2461 ggatcaggaa tgaagatttt aaggatcgag attgctgctt ttggaaaatg gcgtcaaaaa
     2521 agttttgact tttattcagg caatcagctg atctatgggg gaaacgaagc aggaaaatca
     2581 acgatttatc aatttattca agcaatatta tttggtttcc cttcaaaagg ccgtaaaaaa
     2641 aaggattata caccaaaaga cggctcagcc tatggcggca aaatatggct taaacatcct
     2701 gtatatggtg aatttgctgt cgagcgctac aagcagcaaa atcgcggaaa atcgaaggtg
     2761 tggctggggg atcaagtggg aagtgatgag ttactagaaa aactgatcgc acctttgacg
     2821 aaagagttat tccagcaggt atttactttc cagcaagagc agttgatgga actagatcat
     2881 ttgagagaaa aagagctaca tgatgcgctg atttctttag gcatcacggg cagttctcaa
     2941 gtattccaaa agcggcaaga atattatcag aaagcacaac aattattcaa gaaaaaaggc
     3001 caaaaattac taatcaatca aaaattaaat gaatggaaag agctgcaaga aaaaattcat
     3061 caaaaacaag aacaggaagc ttattttggc aagttgatcc aacaagaaca ggaatacgag
     3121 tcgcaacagc agcagttatt ggcacaatta aaagaggcga acaaacaatt ttcattagcc
     3181 agacagcaag aacttaattt ttctttatat gaagaatggc aatctctcaa aaaaaacagc
     3241 actacaggtc ttcctgatga gcaacttctt ttagatctgg agtctttctc caaacggtat
     3301 cagcaaacaa atgagcgttt ggaagaatta gaagcaacac tggaaaagca tagcgggatg
     3361 gatcagcagt cagccagata ttatttttat ttagagcagg agcaggaact aaaagatctc
     3421 cagcaacttc aatttacagc ggaacaatta aacgaagaat ggcaaagatt acaaattgag
     3481 actagagaaa caaattcaga attgaagatt ttggaacaaa ggtggcattg gcagaaaaat
     3541 ccgttaccgg aagaattttt atatgaagaa gaatggaagc atcttctggc tgaagaaaga
     3601 aggattgctg atctagaaag ccagatttct gtgcgtctcc aaatagctaa agaacagcaa
     3661 gagatgattg aagcagaagt gaatgacttg gaagagacct atccagcatt attacaaaat
     3721 cagtcagaga tagcaccagt ctcttcatct aaaccgtata tgctgatggc tggcggagca
     3781 ataatcattg caggtatatt tgtaccaatg cccttgaaag tgatcctttt actaattggt
     3841 gcaggcatga ttggctattt cttctacaaa aggcaaccaa aaattaaaac taaaaatgat
     3901 ttctctgaga taaaaggaat gtggcaagaa aaactaggac aacttgatct agccttagct
     3961 caattagctg aagcagaaga aaaacaaaag agaactggag aagaaaaaca gattcttcag
     4021 caagaagtat atcgttttgc ggatcatcgg cacttaggaa agatggatac gctcaaactc
     4081 atgaaagaat atggacagga agtcatagat tatcagaaat tatatggcgt atttgagcgg
     4141 aaacataaaa gagaacagga aatacaagaa aaactacgac aaatcgacca aaaatttgag
     4201 tttgttaaag aatggttacc gatacaagat aagacaattt cagaaaaaat ggtattgctt
     4261 tcccgtttta tacaagagat ggaacagatg aaatttgcca gaagctatca gcaaaatact
     4321 ttattgaagc aagaaatcaa tcaattgaaa aagaaacaaa aagtatggat acaagaatac
     4381 agcgaacaat tgactcaagc aggcttatct tatccttcgg aagtacctgt ctttttacaa
     4441 aaagccaaac aagccgaaca gcagatgaaa agatacaatg aattatcgga aatgcttact
     4501 ccactatttc ctgaagagct gacaaaagag aaattatctg taagactgcg ccaactgaaa
     4561 gaaaagcaat cccaactcca gcaagaagaa aaagaagcaa gtgaaaatcg gcaacgactc
     4621 caacttcaag tagaagagtt gcaaagagac ggcacattgg acgaacttta tcaacaagct
     4681 agccggcaaa aagcaaaatt acaggaatta ctggaggaat ggggagcact tgaaatcgcc
     4741 gggacattct taggtgatct ttcaacagag atgagtgaaa gacaattacc ccagttgtta
     4801 agagcagcag gtgattattt aggtatttta acaaaaggaa attatcagaa gatattactg
     4861 aaaaatgata cactgacttt aacagacgga tatacagaat ttcccatcta tgagttatct
     4921 acaggtacga aagatcagct gattatggcg atgagatttg cttacctctc attagaagct
     4981 aaccgttcga tatgcccgat catcattgat gatggctggc ttcattatga tcatcagcga
     5041 aagtatcaat tagctgaact tttagctaaa tttggagaaa agtatcaaat aatttgtttt
     5101 tcttctgata aagaaatggt aagctattac catgagatga accaaatcgt aaaacaatta
     5161 gaaggagcaa ataatgaaaa aaattcgtga tttagtaatc gatgagcagt ttgaacaatt
     5221 tgtcctgatc aaaagtgcag atgtaagaat cgctaaaaac ggaaaaaaat ttattgcttt
     5281 tactttccaa gatacttcag gtactatcga tggtaaatac tgggatgctt cggaggagga
     5341 aatcaataaa tttacagccg gcacagtcgt ttttctaaac ggtaaaagag aggtttatca
     5401 aggaaaccct caagtaaaga ttcttcatat gcgcttaaca agggtagatg agccaggaga
     5461 gcctagttta tacatggaaa gagccccttt gaagaaagaa gaaatggaag aagaaatcaa
     5521 tcaattgttg tttgagatca ccaatgcgca ttggaatcgt attgttcgct atctacttaa
     5581 taaatatcaa aaacaatttt ttgagtttcc ggcagcaaaa agaaaccatc atgcatttgc
     5641 cagtggatta gcttatcata ctttgacaat gcttcactta gggaaatcaa tcgccgatga
     5701 atatcctgag ctgaatcgag cattgttgta tgctggtatc attttgcatg atttaggaaa
     5761 ggtgattgaa ctttccggtc ctatgtcgac agaatatacg cttgcaggaa atttgatcgg
     5821 tcatctggta ttgattgacg aagaaataac taaagcctgt cttgcgttga aaatcagcga
     5881 agctgacgaa gatgttctcg tattacgtca tatggtttta gcacaccatg gcttattaga
     5941 atatggctca cctgtacgtc caagaataat ggaagcagaa atattacatc aaatcgacaa
     6001 tatcgatgct tcgatgcaga tgatgttgac ttcgattcgt caaacagaac caggaaaata
     6061 caccgatcgg attttcggta tggacaaccg aagtttctat gtaccaaaag atttatagca
     6121 aaaatttatt tttttcattc aaaacaaaaa aagcgagaca taagtcttcg ctttttttgt
     6181 tttcttttat tttgatgaat cagttgtttc ttcagttgaa gattcttttg ttgaatcagt
     6241 tgattttgtg tcagaactct ttgttgaagc tgtagtttca cttgagtctt tcgtacttga
     6301 tgaagttgtt gtgaatgcac tcaacacatt ttcaaaggca tcatctttga tttttacatt
     6361 tgcatctttt aattcttcac cgattacttt agttgtgaat gcattgtcac ttagtttagt
     6421 ttcagtagcg atatctttta gttgatcttt gtatttatcc atgtcattgc ctttattttg
     6481 gtttttcacc attttgacaa cgtagtattc tgtcgcataa gaagtaggat ttgttgtagt
     6541 aataacatcg gagatctcac cgtctttcaa tttgaaagct gcttctttta cttctgcagg
     6601 aatagttgtt gtcgttgagt cgaatttaac tttaccgcca tcttcttttg tctctgtatc
     6661 agttgatttg tctttggcta atttagagaa atcatctcca tcatccgctg attttttcac
     6721 atcttttgct tcatcttcac ttgataattt gatgatttgt gcttctactt ctggatggaa
     6781 agatttccaa gcagttttca aatcgtcatc tgtaatatct acgtgagctt tcaagccagc
     6841 ttcaaaagct aaattgttac gaatatattc tttataagta tctttcgtat atccagcagc
     6901 ttctaattgt gattcaaaag tatcacctaa tgatttcgct tgtttgtcgt attctgcatc
     6961 cacttgctta tcggttactt tatcgccata tttgttattg aatactttat aaatgatcat
     7021 ccgttgtact aaggattggt tcgatgattc aagttttgct tcatcataga aatcagatac
     7081 agtgatcgtt ccgcctttca tcgttgcgat atctttatta gtatcgcctg aacaagctgc
     7141 tagagttaaa acagccaacg cactagtagc tgccaaaata attgattttt ttttcattaa
     7201 aatttgcact cctgtaatta gttattattt ttatatatta actataccat atcccatttc
     7261 ctttaaaaac gttgtcgtgg tagtttcaaa caaaattcaa aatttcatca cattcctagg
     7321 aaatacgcaa acttttctca cttatatagt taaatctgtt gagaaaataa tcattattat
     7381 atttgttcag gatactctgg cagatcagct tttagttttt ctacttgttc attgatttgt
     7441 gtgatacgtg gttcggcttg gaatttaaaa tcttcgatat cttttttgat tgcttcttgg
     7501 aaagcaggaa tcacagattc agctgtttct tttgtttcga tcaatgcgct tttaaaatga
     7561 ttcaggctgt tatttaaatc atttgtcaga gttgttgctt cttccaattc ttcgatcagt
     7621 ttctttcttg tttcattccc gctcctgggc gccaataaca gacctccgac agtgccagca
     7681 gcagctccaa ataacagtcc tttgaaaaag tttttaatca tcgcaggaca cctgctttac
     7741 gattgtttct tgaatagctt ttagttcttc cgatccataa ctatcttgat gattgacaaa
     7801 gtgaatagaa aaatcatcct ctttgctgta gcgagggact aaatggatat gtgaatgaaa
     7861 aacagattga taggctagtt cacggttgtt attcaagata ttaagacctt tcatttctgg
     7921 aaaagctttt tccaatgctt gagcaatctt aggcacacgc gaaaaaacct ctcctgcaag
     7981 ttcgggatca tattcaaaaa tatctgctac atgacgttta ggcacaacta atgtgtgtcc
     8041 tttcgttgct tgagagatgt ctaaaaaagc atagaccttg tcgtcttcat atattttata
     8101 gcttgggatt tcttgattga tgattttgca aaaaatacag ttttccatat aaaacatcct
     8161 ttctataaag aataaatact tcagcttaac tttaccataa gttaaagcta ttgtaaggaa
     8221 aaaaacaaaa taccgtcatt ttcttcgtgg tataataaat aaaaataaat ggcggaggaa
     8281 aaagaatgag tttaattatc gatcatgtca caggggggta cggacatctc cctgtgttaa
     8341 aagatatcag tttcgatgta cagcctggag agatagtagg gttgattgga ctgaacggag
     8401 cagggaaaag tacgaccatc aagcacatta tcggtctgct ggaagcttca aaaggaacga
     8461 tagaaatcaa tcatctatcc ttaaagaaaa atacggaaga atatcgcaaa caaattgggt
     8521 atattccaga gactccttct ttatatgaag aattgacatt gagagaacat atcgaagtca
     8581 ctgcgatggc ctacgatata cctatggata tagcaatgaa aagagcagaa aagttgttgg
     8641 agacttttcg gttatctaat cgtcttgact ggttcccagc taatttttca aagggaatga
     8701 aacagaaagt gatgatctta tgcgcctttt tgatcgagcc ttctttatat atcatcgacg
     8761 aaccattttt aggcttggat ccactagcta tcaatgcatt gcttgaattg atggtagaaa
     8821 tgaaaaaaga aggagccggt attttgatgt ctacgcatat tttagctact gcagaaaaat
     8881 attgtgatcg ttttgtggtc ttacataacg gagagatacg agcagacggt tctcttgaga
     8941 tgttgagaaa agaatttcag ctgccggatt cttctttgga cgaaatctat attgctttaa
     9001 caaaggagca ggcacatgag tagttttttt caaaaacgtt tagcaagaca tcaaaagaaa
     9061 atgatgaaat atatgcgtta tgtattgaac gatcattttg ttttagtttg tcttttcttg
     9121 attggcggtg tgggattata ttattctaat tggttgaaaa ccctgcagcc accgtttgca
     9181 attggagggc tcataatctc tgtattttgg atgctctgtt tattcgtggg taagatggct
     9241 acttttgcag aggcagcaga tattgttttt ttacttccta aagaaaaaga aatgcaaacc
     9301 tatttggaac gagcttttcg ctattcttgc atttttccgt tgatcttttt gatattggca
     9361 tgcggattgg ccatgccatt agtggtggtt agtacggagc aatccttttc gatgtttttc
     9421 gtctatctga tcatattatg gagtttgaaa ttcagtcatc tgcaaatcaa gagagcagaa
     9481 ttgtttcgac tagatcaaca agttattcgt tattggaaat taggctggtt tttttcttct
     9541 ctgatctttt tgtttctatc attttattgg ctaatatggg caggaatagc aggtgcatta
     9601 gtacaggcaa gtctatatta ctggtttttg tggaagcaac aagaaaaagg attggattgg
     9661 gagaaaatgg tgcatgtaga agaacatagg ttgcatcaga tctatcaatt tatcaattta
     9721 ttcacagatg ttcctgaaat tacggcaaag atcaaaagaa gacgctattt ggatcctata
     9781 ttgactcata ttaaaagaga atcaaaaaac acttatcttt acttgtttgc aagaagattg
     9841 gcaagaggtt ctgattttag tggtctttat ttccgattag taattatagg tgggttattg
     9901 attgccggag tagtcgactt ttattttagt ctgattatcg gtttcttatt tttatatttg
     9961 atcggtttcc aactgttacc tttagcgaac cagttccgct atatggttct cgttcagctt
    10021 tatccaatcc ataagaatca gaagaagcag gcgattcagt atctactcac atgggtatta
    10081 attatcgcgt cggttatttt tggtttgatt gctgcagtcg tattagatgg gcagcagcga
    10141 tgggttccgc tagcggctta tttgattgtt actatgctct ttgttaaact atatgtacca
    10201 actcgtttga agaagatgtc agactgaaaa caaagctgtg tagggtaaaa atgagaatga
    10261 aaaatagagc gttgacagta agaatgctgt tcgttctatt ttttttgaga aaagctggtt
    10321 ttttgaatgg caattaaatg aaaaagaacg tatatgtgct tgacgtgtag tgttcaggcg
    10381 actagaataa taagtactgc aaaacaaaga gtaagaggac gggagaatgc atgatggagt
    10441 ttcagttaga ccaagagtgg cggttacgtc ccatcaaagg cgacacagga aaaacataca
    10501 taggcatgcg tgatgaggac aaagtcttta ttaaacgaaa cacgactccg atgttggctg
    10561 ctctttcaag ggaaggaatt acaccaaaac ttgtttggac aaaacgaacg gggaatggcg
    10621 atactttgac agcacaagag tggctggaag gtcgcttgct gactcctaat gagattggtc
    10681 aaagaaacga cgtgattgat gttttgtacc atttacatca ttcaaattct cttaaaacca
    10741 tgctgacccg tatcggtggt cgtgtcatga cgccggagaa gatgctgcaa aaatatgaaa
    10801 aagaattacc ggaagaactc cagaaaaatc aatttctgca aattgtccat cgttttcttc
    10861 gtgagcagat cccgtcaata gataaggaaa acttatcagt agtacacgga gatgtaaacc
    10921 atcgtaattg gttagtatgt aaaaattatt tatatctagt cgattgggat tcaatcatgt
    10981 ttgcagaccc tgcggtggat attgcaacga ttttaggaaa ttatgtgcca ctgtctagct
    11041 ggaatcagtg gctgatcagt tatggcatac gcccgactga cgaaatgctt gaaaaatttc
    11101 attggtatgc ggccatgaat ttgctccaag aaatcaccag atattgcaaa cgtgaagatc
    11161 atcgacggat gaacgaagaa attttacaat taaaacgaat tttcagtggc tgaggtttgg
    11221 cccaaactct gcggagattt ggccagacct ttttaattag aggaaaggaa tggagataaa
    11281 taatgcgtgt ccgaaatcgt ccgggagcag cggaaatgtt agcggctcac ccgaattttg
    11341 taattagtga tccaacattg tggaagggaa aatggaatga attgtttgaa aacgatcatc
    11401 caatccacat tgagattggt atggggaaag gccagttcat cactggaatg gcaaaagccc
    11461 atcctgaaat caattatatt ggagtagaaa tgcaggtcag tgttgtttcg attgcactag
    11521 acaagttgat cgaacaaccg cttcctaacc tgaaacttct acatgtggat ggatcagcgt
    11581 tgacagagta tttcgctgac agcgaagtag atcaaatcta tctgaatttt tccgatccat
    11641 ggccaaaaaa acgtcatgaa aaacggcgat tgacatataa gacttttctt gctgtagatg
    11701 aacagattct tcgtccaaat ggagaaatcc atttcaaaac agataatcaa ggattgtttg
    11761 agtattctct ggcaagtttt tctcaatatg gcatgatcct caaacaggta tggttagatt
    11821 tacatcaaag tcaatttgaa ggcaatatta tgacggagta tgaagaaaaa ttttcttcaa
    11881 aaggtcaacg tatttatcga gtagaagctc aatttcaaga taagaccaaa aagaaaagtc
    11941 tgtgaaacta actttctgct ttcataaaaa cattaatatc cataaataaa tcccagaaaa
    12001 tgaacattac agatgtaatg tattttctgg gatttatttg atttaaaatg tatttatttc
    12061 atcggttttt ccacaaggtg gaatcaattg gaagcgagaa aatgaaaata atcctttgtc
    12121 ggtgatcttc aattcgggta tgacagataa tgttaagaaa gaaagtgtca aaaaaggatc
    12181 aaaggtttta gctgctccta gttgatgtgc tttagttatt aattcattca actgttcata
    12241 gacgatcgaa aaaggttcct gtgtcatgat tccgcctata ggaagagaaa cacaagctat
    12301 ttcctgctgt ttgttgacaa cggtcatccc gccacctttt tgaatcaaaa gattagcagc
    12361 atacagcata tcctcgtcgt ttgtcccaac gacaactaga ttatgcgagt catgggcaat
    12421 cgttgtcgct atcgctcctt gtttcagctg aaatcctttc actatgccta atccaatgtt
    12481 tcccgtttga tggtgacgct cgatcaccgc aatctttaat tggtcttttt cgattgaagg
    12541 aatgaatcgt tcgccttgtc ggtccacttt ttctaccaaa tctttagtca ctagactatt
    12601 cgggataatt tcgatcacat gggcttgtga agaagaaaga agcagctcta acgaatgtgg
    12661 atttaatggt tgagcgttta gttttggcag acaacacgta tatgaatttg aatgatcagc
    12721 aaataaatgg tcttggagtt gcccagattg aacaactaat gttcccttat agaaaacttc
    12781 atgaattggc agcttggtaa gatcgtctgt taagaagaaa tcagcttgat acccaggggc
    12841 aatcgcacct agtgttttca aatcaaaaca ttctgctgca ttcaatgttg ccatctgaat
    12901 ggcttgtaat ggatcgattc caagtgcaat agcttgacgg acgatatgat cgattgatcc
    12961 ttcttttact aaatcattga tcagtttatc atcggtgaca aagaagcatc ttcttgaatt
    13021 ttcaggtgtg atcgccggta gtagcgatgc cagatctttt gcaacggtcc cttctcgaac
    13081 cattaaatac ataccaagat ccaaacgttc tttagcttct tttgcagttg tagcttcatg
    13141 atccgtccga atacctgctg caggataaac atttaactct tccatgccga taccagctgc
    13201 atgcccgtca atttttttct tatgctggtg catcaattgg atcttttcta ggatgtctgt
    13261 ttcattattt gctactgctt gatagttcat gacttcagct aagcctaaaa cttcgggttt
    13321 ttgtaaaaaa ggggctaatt tttttgcatt taaatttgca ccattggtct caaaaggggt
    13381 tacaggtaca caagaaggta gcataaaaaa gatattcatg agtgtttgtc ttgcatcttc
    13441 tatcataaac tcaagacctg tactcccagc aatgtttcct atttcatggg gatctgtcac
    13501 tacagtagta actccatgaa gcaaggatac ttttgaaaat tcgcttggtg ataaaagtga
    13561 gctttcgata tgcacatggc catcgatgaa tcctggcact agatattgcc cattagcatt
    13621 atgtacgctt tttccttcgt attcaccgat tcctacgatc attccttcgc agattgctac
    13681 atctttttcc atgatttctt tcgtaaagac gttgacgatc ttagcatgct tgatcactag
    13741 gtcagcaggc ttttttttac ttgctacttc gatatatttc cttaatgttg tttccaatgg
    13801 agtgcctccc aagataaatt tttataagta taacataaat tcggtttttg ccaatatttt
    13861 tttgtaaatt cattttattt gaaacaaaaa gatcaaaaag acgtatgttt tttgtggagg
    13921 ggagaataat ggaaataaaa cgattatcgg atcaaatgat aagtgaaagt tttgccaaac
    13981 atgcccaaga cgtatatata ttgccagctt cagaagtatt ttacatctat tttcgctatg
    14041 gaaaaaaacg aacattctgg aagaaagtct cttcggcaga tggacagcaa ctgatctcaa
    14101 gatttaagta tttgggacag atggatatcg gagaaaaaag aaaagctcag ttaggtgcat
    14161 ttacttattc tttagaaaaa caggaagttc gtctgcggtt gtcttctgta ggagattacc
    14221 ttcaacgaga gagtttagtg attcggctat tatacggtac ttttgaaaca ccccatcgtt
    14281 gctttagttc tcgagatttt gcaactattt tacagaaaac cgtcagacgt ggattatacc
    14341 ttttcagcgg accagtcgga tcaggcaaga cttctctaat gtatcggttg gctttggaag
    14401 aaaaattgca agtgattact atcgaagatc ctgtagagat agaagaaccg ttttttctgc
    14461 agctacagat caatccgaaa atagcacaga cttatgatac acttttgaga ctcgcattac
    14521 ggcatcgtcc agacttgcta atcattggcg agataagaga tgaatcaact gcacatgctg
    14581 cggtaagagc agcactaaca gggcaccggg tttttgcaac cgtacatgca cgcaatcttg
    14641 aaggtacatt ggtcagaatc catgaactga caaagaaaaa agaagagcta tctgaatgtt
    14701 tagctggtgt catttatcaa gaacttttgg aagatgccaa tcaccatcct tctgttttat
    14761 ggagttatca atttatggag gaagaatcag taaaaaaatc atgggaggaa agttatgaag
    14821 aagctcagga aaatatttgg cagtcagaac tattctaaag gattaacaag aagccagcaa
    14881 aaccagtttg cacaaaccat gggagaactg cttttatctg gttttagtct gcaagaatcc
    14941 ctttatttat tactcacgat ccgttcgttt ccacctgtta ttttggcaaa tatccaacat
    15001 cagctaaagg aagggcaagc gtttagtgag gtactttcgc aaataggata tggagcagag
    15061 caactggtac agatcgagtt agctgaacgt catgggaatc tgccgacaac tttgttggaa
    15121 attgccaaac agtttcggct tgttgaagga tttcgcaatg atttgaaaaa gctgcttgca
    15181 tatccgatgc tattgctcac ctttttgcta ggtatcttgt ttagtatgcg gttgttcgtt
    15241 ttgcctcagc ttattggttc aggaatggtc atgcaagagc attggggtat tcagctgata
    15301 caagtctttc attggtatgt tctactattc atcctagcgg ttactctatt ggctttacta
    15361 gtaaggtatc gtctgatgaa gttgtctcct gttgatcaga gtgaatggat aagcagacaa
    15421 ttattgattg gaccgatata ttccttgtat caatcggctt atttgtcact agagattgga
    15481 aagctttttc atgaaggttt cgaattaagg caaatcatag tctgcttaaa ggaaacgaaa
    15541 caagaaagtc tgattcagca tcttgcctct aagttgattc aaggattgga ggcaggtatg
    15601 tcattggctg agcagttcca gaattatcgt tttctatcag aagaattcag taaaattgtt
    15661 ttacagggag aggcaaaggg aagtttaggt aaagaactga ttttttacag cgaatggaca
    15721 agaaaacagc ttttcaaacg tcttcatcac tggatgcatt ttattcagcc aatcattttt
    15781 ttgggcgtag cggctttgat tttattagtt tatgccgctg ttttattacc tgtttacgga
    15841 agcatagagg aggttttgcc atgaaaaaga gaaaaatatc ttattatagc ggttttacat
    15901 tgatcgaaat gctaatcgtc ttactgatca tcagtgtatt agtgttgttg ttcgtcccta
    15961 atttgtcgcg ttataggaat catgtagacc aagaaagtcg tgaagcgatc atccagttgg
    16021 tagacacaca aaaagagctc tatgcgcttc aaaataatgg aagagttcct actgtggagg
    16081 aattgttgaa tgaaggctat atcaaacgag aacatgcaga aatctatcag cggccttgaa
    16141 ggtaaggttt tagcatcagg atatacgttg tttgaatcac tcttactgat tttattactt
    16201 acaacgatcc tttcttttcc agttctcgca ttttctgcat ggaagaatga attggcagtc
    16261 tatcaatttt tcggccaatt tgagaaacga atctattcta cgcaaaaaat cgctattgtg
    16321 aatcagatcc aaacaggctt ttattggaat agtgaagaga atcaaatcat ttttcatgtt
    16381 ccgaatccag ataaaataaa ttgggaagta ttagatattc ctgatgagat taccttgaaa
    16441 cgccatgctt ctattacttt tgctgctaga accgggaatg aaagttcact aaaagcttat
    16501 caattttatt gggaagaaaa agagcggacg attacgtatc aattccagat agggagtggt
    16561 cgctatacaa aaagaattga ataaaacagg atttgttttg ttggagtccc ttacagcgct
    16621 tatgatcgct tctgtctgtg tctacttagt gagctttggc cagctgtttt tgcttcaggc
    16681 agaaaagaaa agtggggagg aactgctatt tattcgaatg atttatgaag atatcaagac
    16741 gcagcggatg tatcacaaaa gaccagagcg aagggaagaa atgatcccat acgatatcca
    16801 aattgtcgat aataatggag taatcgaaaa agtgtttgtc aatatggaga aagaagcgat
    16861 cagtattgaa aaaacaagta attccagcat ttacgctgat tgaatgtcta attggactga
    16921 tgatattgag cagtctattg ctcacattca atcttttgat tcagcaaacc atccaagtaa
    16981 gtgaattttt gcagcgtaaa gatcaaaaag agtggctaat atttttagca caattagaag
    17041 aagaactcaa gagcagtcac aatgtctctg tgaaaaatca gcaactgcac tacacgatcg
    17101 aggataaaca gtatatcatc gaaagatatc aggcaatgat acgtaaaaga agaacttccg
    17161 gcggacatca acctatgtta acaagtatca ctgagcttca attattggaa aaagaacaca
    17221 caatttcttt ttatgtacac tttgaaaatg gagaagatgg atatgccata tggacaaaaa
    17281 acgatcagta gaaaatgtta taagggctct attttatttt ctgtcttgct tttatttctt
    17341 ttatgtacgt ttttattgat ggcagcatta gaaagctacc gtttgtcaat ggatcttaat
    17401 atacgaatca aaaattatta tatagcaaaa ataatgaagc agatggcttt atcggaacag
    17461 tctacgcttg ccgaaagttc ggaaggagtt ttttactaca caactggatc tgtaacatat
    17521 cagtgggtac aacagtccct attcttagag gtggaagtct caccgtttat ctttcggttc
    17581 acagaagaag taaaaaatga tacagatact agtacagaat gatggaatgt tcgtgaaaac
    17641 gccctctttt ctgttataac actgttttta ataaaaaaat gtttgccgtt tcacaaataa
    17701 aaaacagtga aaacgattga aattttgtca attgttcgat agaattaaac aggtgaaaaa
    17761 attgttataa tatatctaga aatgaggtgc accatgttac cagaaaaaat ggaacaaggg
    17821 tttaatcaaa acctggaagc tattcagctt ttacagaatg cattgggaac ttccttttta
    17881 gaagcctatg tcgaaaatgc agaaaattta atagatgatt atcaagtacg tgtggtggat
    17941 ggagtgccga caaaagagac aacgcaacgg atcacagctc tttatgaaga gttgaagaaa
    18001 ctttctttcg agccagaaga atggcgcaga ctgtctcaat tacttttatt gaagggcagc
    18061 cagacagaac atttgcaggc gaaccatcaa ctcactcctg atagtatcgg ttttttattt
    18121 gtttttctta ttgaacaact atacacaaat aaaaaagcac cggttaagat tctggatatt
    18181 gcggcaggaa tgggcaactt gttattaaca gttcttttaa acctatcgaa tgcggggtac
    18241 caaacagaag gaattggtgt ggatattgat gatacattgt tggctgtcgc agcttctaca
    18301 agtgatttga cacaagcaaa tgttcaatac tttcatcaag acgggttgca ggaattatta
    18361 atagatcctg tcgattttgc aatcagcgat ttgccaatcg gttattatcc gaatgacgaa
    18421 aaagcaaaag agtttttaac aagtaccgaa gaaggccata gttatgcaca tcatttattg
    18481 ctagagcaat caatgaaata tgtgaagccg gatggttttg gtttgttttt gatgccgagt
    18541 ggatttttag aaacggatca aagtgaagaa atcaaaaaat ggtttaaaga agaaggttat
    18601 cttcaaggaa tgattcaatt gccagatgaa ttgttccgta ataaacaatc gcaaaaaagt
    18661 attttgatct tacagaaaaa aggaccacaa gcacaacaag taaaagaagt cttgcttgtg
    18721 aaacttgctt cattaaaaga accagagaag gtgacggaat ttttcaacga gtttaaaaac
    18781 tggaaatcat ctgctctgta atcattcaac gagaaggaga gtcatatgtc taaaacaatt
    18841 gcaatcaatg ctgggagttc aagtttgaaa tggcaattat atcaaatgcc agaagaaacc
    18901 gtagttgcga aagggatcgt cgaacgtatc ggtttacaag attctatttt taccatcaag
    18961 tacggcgata atcaaaaatt cgaacaaatc atggatatcg aaaaccacga agtagcagta
    19021 aaaatgctgt tagatcagtt gattgaactg caaattttaa gctcttatga tgaaatcacc
    19081 ggagtagggc accgtgttgt tcacggagga gaaacttatg gggattcagt agtgatcgat
    19141 gagaaagtaa tggaccgcat tgctgaatta gcagaatttg ctcctttgca taatcctgca
    19201 aacttaatgg ggatcaaagc gtttaaaaag attttacctg acattttgag tgtggctgta
    19261 ttcgatacat ctttccactc aacaatgcca aagcaaaatt atttatatag cttaccggtg
    19321 tcttattatg aagatttcaa agtaagaaaa tatggcttcc atggcacaag tcatcgttat
    19381 gtgtcggaac gtgcggcaaa attattgaac aaaccgatcg aagaactaaa aatcatcact
    19441 tgccacttag gaaatggtgt atcgatcaca gcagtcgatg gaggtaaatc catggatact
    19501 tctatgggat tcacaccatt agctggggtg acaatgggta cacgttcagg tgatatcgat
    19561 ccagctgttc tcccatattt aatggaaaaa ttagagattg atattgatga aatgatcaat
    19621 gttttgaaca agaaatctgg tctgctaggc ctaacaggat tgtctagtga tatgcgtgat
    19681 cttgaaaaga actatgaaaa agaacatatt cgtttagctt atgacatttt cgtggaccgc
    19741 atccgaaaat acatcggcgg ttatgtgaca acgatgaatg gtgttgatgc aattgtcttt
    19801 actgctggta tcggcgagaa tgacggacat gtacgtagtg aaatcatcaa aggtatgtct
    19861 tggtttggtt gtgaaatcga tccagcgttg aatgaattgc gtggtgaaga agtagatatc
    19921 tctacaaaag aatcaaaagt aagagtatta gtgatcccaa cagatgagga actaatgatt
    19981 gcccgtgatg tggagcgtct gcgcggctaa gccaaagcat tcgttaggat aaaagatgaa
    20041 acgcatgcaa gagggaattc ttgcatgtgt tttttttgcg gagaagaccg caaaatggta
    20101 atttaacttc aatttccctt cttttttaca ggatcttctt agggaatttt gcttggaaaa
    20161 gtggtcaaaa gggaaaagac tggcatttta tttattgatt ttcaggaaaa tcccaatatt
    20221 ttattttcat tcatctttat aagggacatt acatatgtaa aaagaaaaaa catttttaga
    20281 tcagcctcgt gggctgtcga ctttattctt cgctttatgt ttagctagaa aaccgatccg
    20341 aaacattcat tgatcgaata ataaaaactt gattttccac ctgtactttg cctgaatcgt
    20401 gaaagcacag gtatgaaaat caagtttttt gttgcgaaaa aacgtcctag tcaatcagtt
    20461 ggccgagcgg ctggcggctg atctgctcct gatcaataac gagtgcttct tggaagtgtt
    20521 gctgcaaaaa gtagcggtca tggctgactg ctaggagcgt tccttgatag tcttgcagca
    20581 tttcttcaat ttcttctcga gcatagctgt cgaggtgatt ggttggttca tccaaaatca
    20641 ggacgttgat tttttctaaa aagagtttca gcaacgccaa acggattttt tcaccaccgg
    20701 ataaatcttt gatccgttta cccacatcgc tttgataaaa gccacttttg gctaattgcc
    20761 gtcgagcgac ttcttcgttt ccagtcaatg tgatggcgta agccagcaaa cggctatcgg
    20821 gatcggcaaa ctgcagctgc tgcggcaaat aaccgatttg caagctacta cctaatttga
    20881 tggtgccacc gtaaggcttt tcctcaccga gtaataattt gagtaaggtt gattttcctg
    20941 caccattgtc gccgatgatg gctaaatgat ctccacgata gactgcaaaa ctgctgtctt
    21001 tgaatagtac ttgatcagcg tactgctggt gcaagttttg aatgatgaaa acttctttgc
    21061 cagattttcc accattagac aagctttgca gccgattttt cgggggaggt ggcggtttga
    21121 cgagtgtcag tttcgccagg cggcgttcca gttctttggc ttttttgaag aaggactcat
    21181 tgtctccttc atgtgcccac tgacgaaaac gacgaatcat gactttcaac cgctggactt
    21241 ctttttgctg cagttcgtaa ttttttgtca gctcagccaa gcgagcttct ttcaagactc
    21301 gaaaacggct gtagtttccg ggatactcaa tcaatgcacc ttcttccagt tcccatgttt
    21361 ttgtggtgac ttgatcgaga aattgccgat catgagaaac gaccagaacc gatttttttg
    21421 tattctgtaa atagttttcc aaccagcgaa tactgttaag atctaaatga ttggtcggtt
    21481 catcgagtaa aagtaaatcg gcatcggctg ctaaaatttt ggctaaagcg actttaactc
    21541 gttcgcctcc gctaagttct gttaaccgtg cttcctgacg gtgtggaatc cctaagcctt
    21601 gcaagatcgc tgcaatccgg tcttctaata cgtaaccgcc tagttcttca aagcgatgct
    21661 gcagctcacc gtaacgggcc agacacttct ctaagtttgt atctggttct gtcatcaatg
    21721 cttcgtagcg actcatttgc tgtttgaggt cctgtatctc agcggcagaa tggagcaagt
    21781 agtggttgac ggtcgcttcg gaaactgtaa gttcttgttc gaccgtcccg atctttaagc
    21841 cttttttgcg actgataaca ccgctgtcga caccttctct gccggtcaag atttgtaaaa
    21901 aggttgattt gccagttccg ttgataccga ctaaggcaat tttttcacca gcttcaattt
    21961 gacaggataa atcctcaaat aatggattcc cgccgattgt ttttttaata gaaattgctt
    22021 gtatgatcat gatctctctc cttgagacca ctcaaaaagg ccatggttat ccatggcctg
    22081 tgttatttaa actcagatta tcatgagaaa aataccttga gatggccatt gattttacgt
    22141 gaattcaact gttttggaca aacttgtccc gtcaacagca actgtaaaac caattgcatg
    22201 gctaaatgcg atatggtatc gttggtaatt ttcattactt gttattttag tcatgcgcat
    22261 catagtattt tccctccttc gtgtagttgt cttttccagt gtacctgaaa gtgtcagaaa
    22321 gttcaactag cgctgacatt tacttaatat ttatcgatgt gtaattctgt cagagagctt
    22381 tttttatctg actgagggta atgtctcgtg gaccacatca ttgtattctg agctaaacca
    22441 gcgttcaggc gaaatcatat ggtgtttgat aaaaaacttt gtctacatga cttccttttg
    22501 ccatttgctt aataccgttg cattaaacag ctcttctctt tggaaatctc accaacatcg
    22561 cgttcaggag tatcgttgtg gttgcaatgg caagcggtag cctttcatca attcttgaat
    22621 gatttaccga atgtagcact tgccttctcg cggtacccga acccgaaata aaattttagt
    22681 ttggcgataa aatcggtatc cgccatcgat acttcatatt ttttcgtatc agattcatcg
    22741 gaaacttcca cattgacgaa ccaataagct ttggcatgct taggtcgttt taaaccacac
    22801 cagggcgact aatcgccctg gtgttcactt ttttttattt tacttgaact cattgactgt
    22861 tttcccttta ttttaaacat tataaagacc gatgagtgct tgccgcgtct tttgttttta
    22921 tcacggtttt ttgtgcaaaa aaacctgccg atagttggca ggtcgtctac gtgttaagct
    22981 ttggtgtggt ctttagctgg caattggttt tctaaatcag ttcggacaac aagcacatcg
    23041 caagctgcgt ttctaataac atattcagaa acagaaccaa taaataaacg ttctacagca
    23101 ttcaatcctg tggctcctaa cataatcaaa tctacttgat tatcttctgg gatttctctg
    23161 gcaataattt gttttggtga gccatattct acgacagagg ttacattatt caagccagct
    23221 ttcttcgcat tgctttcata atctgccaat gtttgctttg ccatttcagt agcttgttct
    23281 gcaagcattc catcaaagga tgaaacggtt tggaaagaac gtgtgtcaat gacatgtgct
    23341 aatagaagtt ctccgttatt tcggatagct acattgaccg cctttttaaa agctaattca
    23401 gcttcatcag aaccatctac ggcaaccatg atttttttgt attgttgtaa cataagcatt
    23461 cactcctcaa gtcaatcgta ttgtactctt ttattgtaga acactttagg gaaaaagaga
    23521 attatttact gctttggttc agcaattttt tttgcaaatc ggatactgaa ggtggcagaa
    23581 acaataatta cgatcagtaa aaagaccaag gcaataaagc gttgattgaa aaaagctgtt
    23641 aaaataccca ttacgcctaa aacagcatat gcagctccgt aaaattgtag gaatccttga
    23701 tttttatcat ttttgttgat caatacaaaa aagatatcct gtttattaaa taaataatag
    23761 ctaacgaata ataatactaa agtggcgata atcattaaaa tcctaatcat gaacaaatcc
    23821 tccaaaataa gcaacaaata gaaaaagcaa tttgccaaga gcaaattgct ttattcttta
    23881 aaagatggta atctccgtta atgccgttgt ttgtcctgta gtattgatcc cgcgaattca
    23941 agcaggtggg tcccatggtc gtagcttcga actaccaaat gataaggcat gttaccagca
    24001 gtgacacaag acgggtccca agatatcaat aaaaatgttc ggtcaacaca caaagaggac
    24061 aattcgaaca tcacagattt cccacctcta tagtagcata tgaatttaaa aagagcaatg
    24121 agagagctcg cagaaacaga accgttttct tgtctagttt tttggaggat gctttttcca
    24181 ctcttttata cggtaatact gttgacccag agcctgttca tatttccctg tatcttttgg
    24241 ctgataatac tgagcattct tcagtttatc tggtaaatat tgctgattga cccaagcctg
    24301 atcaaaatga tgaggatact gataacccac gccacggttg agtgatttcg cacctttgta
    24361 atggctatct ctcaaatgat caggaacatc tccagctttg ccctctcgaa tatcagcaag
    24421 tgctgcatct aatgccatgt aggcagaatt ggattttggc gacaagcaaa gatcgacgac
    24481 gacgtctgct agtgggatac gtgcctcagg caatcccaat ttttcagcag ctaatacagc
    24541 gttcactgta cgagccgcag cagcaggatt acctaaacct atatcttcat aaccgataac
    24601 catcagacgc cggcaaatac ttgccagatc acctgcctca actaaacggg cgagataatg
    24661 aagtgcagca tccacatcac ttccacgaat cgatttttga aaagccgaaa taacgtcata
    24721 atgagcatct ccatttttgt catgggtaag agcttttctt tggacacatt cttcaatgat
    24781 cgacagtgta agatgaatcc ttccttcttt atctgaagga gtcgatagtg tagccagttc
    24841 cagcccattc aatgcactcc gcaaatcgcc gttcgttgca cgcgaaaggt gaagaagagc
    24901 atcttcgtct aactggattg cttgttgtcc aaggccgcgt tctttatccc ttaaagcatg
    24961 ttcgactgcc aactggatgt cttgttctgt taatggtttg acttcaaaga tctgtgtacg
    25021 gctgcggatc gctggattga tggtaatata aggattctca gtagtcgctc cgatcaagat
    25081 gattcggcca ctttctaaat gaggaagcag aaaatcctgt tttgttttat ctaatcggtg
    25141 tacttcatcc aataagagga tcactgtacc actcattttt gcttcttccg ctacaacttg
    25201 tagatccttt tttgaatctg tagcggcgtt tagcatgcga aatgcataat tagtagagcc
    25261 ggcgatcgca cttgcgatac tcgtttttcc agtaccagga ggtccatata gaatcatgga
    25321 agagagcatg cgagcatcga ccatgcggcg tatgattttt ccttccccga ctagatgctg
    25381 ctgaccaacg acttcatcga tcgttcgagg tctcatgcga taagccaatg gctgttgcat
    25441 aggattttcc tccttcttgt ttttacgtcc cttaattata tcatatttag caagatgcct
    25501 agaaagaaca gccttctgtt gttttgtttt catagcttat ccaactaata ttgtgtatga
    25561 taaagtaggt gagaaaaatg acggataaac aaaagataga tgaatttttt tggaataaaa
    25621 cgacgccaga agtggcacgg gatttattgg ggatgtattt ggagcatgac acaccagaag
    25681 gaagattagc tggatatatt gtagacgcgg aagcatacct aggtccagaa gacgaggccg
    25741 cccatagcta tggtttaaga agaacaccgc gtgtgcgggc aatgtatgaa aaaccaggta
    25801 cgatctacct ttacacgatg catactcatc ggattttgaa catcattact cagccagaag
    25861 ggattcctca aggagtaatg atacgtgcaa tcgaacccgc cgcaatgatt gatcaaatga
    25921 gtaaaaatcg tggcggaaaa actggtccag atatcagcaa cggtccggga aaattagtgg
    25981 aagcattagc aattcctcaa gaactttatg gtcagtctat agcagatagt tcgttacgct
    26041 tagtttttga gaaaaaaaag acaccaaaaa agatcaatgc tttgcctaga atcggtatcc
    26101 caaacaaagg tgtatggaca gaaaagccgt tgcgttttgt tgtgtcggga aatccttata
    26161 tctctttaca aagaaaaaac caaatcgaga aagattgggg ttggaggaaa gaaaatgaaa
    26221 aagaaggatc tattaacata tttagatgaa ataattgaaa aacaagtgac agactatgat
    26281 gtagctttag attgggatac aaaaaatcat acgatcgaga tcgttgttcg cttgtttgca
    26341 gaaaataaag cacaatttga gattgatgat gcagaaggga tcgtttcgca agaaccaatt
    26401 atcgaatttg aagacggcgt acttcttttt aatcctcaga aatctgtttt tgatgaacgc
    26461 aattatctag cagtaatccc ttatgaagga aaaaaagggt tggcaaaatc agtagctgat
    26521 tctttagtgg aatatctaaa tgaagtatta gctcaagggc aaagcgattt gcttgatttt
    26581 ttggatgaag acgatgacga agctgtattc gagcttcact gggataatca gcaactagca
    26641 gaattagtag aagtaaaaca gcaaaatgaa gcagatactt atttaccgta cccaagttat
    26701 taaggagtga cagatatgaa atggaatgaa gtaaaagtag aaactgaaag tgaagcagtc
    26761 gaagcagttg ctaatatttt gatggaggca ggggcgagtg gtgtcgcgat tgaagattct
    26821 ttagatgttg aacattttca gtcggatcaa tatggtgaga ttttagataa aaatgatttt
    26881 acatcgctga aagaaggcgc actagtcatg gcttattttc cggaaacgac tttcttaccg
    26941 gaaatcttgc cttttatcaa agatcgtatc acacaattac ctgagtttgg tctagctatt
    27001 ggaaaaaata aagtaacagt tagtgaagta gaggaaagca actgggcaac agcctggaag
    27061 aaatactatc atccggtgcg tattacgcga atgctgacgg ttgcgccaag ctgggaaaac
    27121 taccagacag aagatcctct agaaaaaatt atcacactag atcctggtat ggcatttggc
    27181 acaggcactc atcccacgac ttccttgaca ttgacagcac tagaaacagt cttgcgtggt
    27241 ggtgaaacat tgctagatgt gggaactggg tcgggcgtgc taagtattgc agcaaaatat
    27301 cttggtgcaa aagatgtcta tgcctttgat ttagacgaag tagccgttcg ttcggcgaaa
    27361 gaaaatatgg atatgaatga aatagccaaa gatgtccatg tatcagctaa cgatcttttg
    27421 acaggaatcg agattgaaag tgatgtaatc gttgcgaaca ttttagcaga tatcatctta
    27481 ttgatgatcc cagatgcttg gcgtttactg aagcaaacag gcacgctgat cgtttcagga
    27541 atcattgaag agaagaagca aatggttctg gatgccatgt tagaacaagg gtttatcgtg
    27601 gatcaaattt tccagcaaaa agactggtat gcaattattt tgaaaaaacc agaggtggaa
    27661 tagatgcagc ggtatttttt aaaagaatca tatgaatcaa aagactattt tgttttagaa
    27721 ggggaaaact atcaccacat tgtacgggtt atgaggatgg aacccgagga tgaagttttt
    27781 ttggcattca atgatcaaat agccattgtt gcagaaatca cggagattac tgaagaggct
    27841 gtttatctga aagaaattaa aaaagaagaa aatcaaaagg aactgccaag ttcgattacc
    27901 atagcgagtg gctatcctaa aggggaaaag ctggattgga tcgttcagaa atccactgaa
    27961 ttaggagcgc aagcttttct tggtttcccg gcatcagcct ctgtcgttaa atgggatgca
    28021 aaaaaacgta tgaaacgaca acaacggctt gaaaagattg ctcaagaggc agcggaacaa
    28081 tcccatcggc agatcgtgcc atcagtagag ttattggaaa cgaaacaaga tttatttgag
    28141 cggctagatg attatgacgt ggtcttggtt gcttatgagg aatcagcaaa gcaaggcgaa
    28201 aaatcaaatg cagccaaaat tcttagtgaa acaaaaccag atagtcgaat cttagctgtc
    28261 tttggaccag aaggtggatt atcccgcgat gaagtcgatt attttgtaga acacaaaggg
    28321 atactttgtg gattaggacc aagaatcctt cgtacagaaa cagcacctct ttatttatta
    28381 agtgctgcta gctatcaatg ggaattaatg aatccttctt tcagctaacg ttcttcatta
    28441 ataaaagagt atcgacagaa agaattgaaa aaatagtcaa caatttgtat aatagaaaca
    28501 attatttata caatggatac gaaaaatcgt ttgaaaaagc ttttaaaaag cgaacatttc
    28561 cgtaaaaaaa cgtataactg cacggatttt gggacaatta tcaaacagat atttgtcccg
    28621 attcccgttt taccgaaaaa agaaaggggg atcgattcct gtgacaaaag aagaaatttt
    28681 aacgggaccc ggtgtcataa agctcgttag tcaatacatg ggtcccgaac atgtagcatt
    28741 tgttgagaaa gcatgcgaat acgcgactgc tgcacatgac ggacagttta gaaaatcagg
    28801 cgaaccctac attattcatc ctatccaagt cgcaggtata ttagcagatt taaaaatgga
    28861 tccccataca gtggctacag gcttcttaca tgatgttgtt gaagatacag aaatcacttt
    28921 agaagatctg agagaagaat ttggcgatga cgttgctatg ttagtggacg gcgtaaccaa
    28981 attagggaaa ataaaatata aatcccacga agagcagcta gcagaaaacc accgaaagat
    29041 gctgctagca atggctcaag atttacgagt tatcatggtc aaattagctg atagactaca
    29101 taacatgcgt acgttgaagc acctgcgaga agataagcaa agaaggatcg ctcaggaaac
    29161 tttagaaatc tatgcgcctc ttgcacatcg tctagggatc agccggatca aatgggaatt
    29221 agaagataca gcacttcgtt atctaaatcc aaaacagtat taccgtatcg tccacttgat
    29281 gcagacgaag agagaagaac gtgaaaaata cgtaagtggt actgttgaag atattcgaat
    29341 agcgacagaa gagctgggga tttttgcaga aatctatgga cggccaaaac acatttattc
    29401 aatctatcgt aaaatgaaag atcagaaaaa gcagttcaac gaaatttatg acttgctagc
    29461 tattcgagtg atcgtagatt caatcaagga ttgttatgct gtattaggag caatccacac
    29521 aaaatggaaa ccaatgcccg gcagatttaa ggattatatt gccatgccaa aagctaatat
    29581 gtaccaatct ttgcatacta ccgtgatcgg tccggcagga aatccggtag aaattcaaat
    29641 ccgaacacag gaaatgcatg aaatcgctga attcggggtt gctgcacact gggcctataa
    29701 agaaggaaaa aatgaaaaag tagaaccaga tggtatgacg aaacaattaa gctggttcca
    29761 tgagatactc gaacttcaag acgaaagcta tgatgcttct gaatttatgg aaggcgtaaa
    29821 aggagatatc tttagtgata aagtctacgt cttcacacca aaaggagacg ttactgagtt
    29881 accaaaagga tccggaccat tggactttgc atacagtatc catacagata tcggtaacaa
    29941 aaccactggt gcaaaagtaa atggcaaaat ggtgcagctt gattacaaat tgaaaaacgg
    30001 agatatcatt gagatcatga cttctccaaa ttcatttggc ccaagtcgcg actggttgaa
    30061 attagttgct actagcaaag caagaaataa gatcaaacgt ttcttcaaag cccaagatcg
    30121 agaagaaaat gtgatcaaag gccacgaatc cgtggtcaaa tgtattacag atctaggatt
    30181 tacgcctaaa gatattttga cgaagaacaa actgcaagaa gcactcgatc gttttaatta
    30241 tcaaacagaa gatgatctct atgcagctgt agggtatgga gaagttagcc ccttgacgat
    30301 ggccaatcgt ctgactgaaa aagaacgtaa agaacaaaaa atcgagcagc aaaagcaaga
    30361 agcagaagaa atcatgaatc agccgaaaaa agaacctgac aaaatgaaag tacgtcatga
    30421 aggtggcgtt gtcattcaag gggtagaaaa cttactaatt cgtatcagtc gctgctgtaa
    30481 tccgattcct ggtgatgata tcgttggtta tatcactaaa ggcagaggga tatccattca
    30541 tcgtcgagat tgtccgaatg ttcagcctga caaaccaaat gtagcagaac gtttgattga
    30601 agtcgaatgg gaagatacat cgaatacacg aaaagagtat gatgcagatt tggaaattta
    30661 cggctataat cgttcaggct tattgaatga tgtacttcaa acagtcaatg cgctaacgaa
    30721 aaatctcaac agcgttgaag cacggacgaa taaagataaa atggcgacga tccatttgac
    30781 ggttggtatc cagaatttat cccacctaaa gagtatcgtg gataaaatca aagcagtacc
    30841 tgatgtctac agtgtacgcc ggacgaatgg ataggaggac agatatgaga gcagttgttc
    30901 aacgggtaag taaagctgct gtgtcagtcg atcagcaaga aattggaaaa attggctacg
    30961 gcctacttgt tttattaggc attcatgaaa ccgatacgca aaaagatgtg gactatctgg
    31021 taaaaaaaat tgcacaaatg cgtatttttg aagatgaaca aggaaaaatg aatattagtg
    31081 tagaagatgt gggaggacaa attttaagta tctctcaatt cacactgttt gcggatacga
    31141 aaaaaggaaa tcgaccaagc tttgtggaag ccgcacgacc agaaacagct attcctttat
    31201 acgaatcatt caatgaagga atcagaagtc gcggcatcac agtcgctacc ggacaattcg
    31261 gagcagatat gtcggtttct ctcgtcaatg atggacctgt aacgatcatt attgattcta
    31321 aaaacaaata aagtaatctg gttttcacta gtaaaaacta tgaaacacgt cgcttaagtt
    31381 agactgataa actctaactt aagtggcttt tttgtctgca aaaagcgctt cttttgtcag
    31441 cagtcggaaa aaaagttaag cttaaattat acaagttatt ttttatagga ggttgctgaa
    31501 gatgaaagaa gtaccagtag tagatatcaa aatcacagat ttaaaaaaat tgtatcaaaa
    31561 aggatacaac atgttggagg agttacgcca tgaagcggac gctcccgtcg tcaaagcaaa
    31621 gattttcaat aaagaagcta tcacgatcta cggatcatca gcagcaaaag tattttatga
    31681 tccaaggaat ttcaaacgaa aaggtgctat gccaaaatta gtattgaaga cactttttgg
    31741 tcaaggtgga gtacagactt tggatggagc tgctcatcac catcggaaaa atatttttat
    31801 ggatctaatg acaccggaaa gaatggaaga ttatcatcgt atcttagata agaatctgac
    31861 acaagcactt gaagcacagc atggtcaatt tgaactattt gatttaagta agatggtttt
    31921 ctttacatct atttgcgagt gggcaggtat caatttatca gcgatttcta aagatgaggt
    31981 cgaaaaatta gcagaatatc aaatctctat gatcagcgga acgtttactt ctccgattga
    32041 tcacataaaa ggtgtcgaaa atcgcaaaaa atcagaaaaa tgggcgcaag gcttgattga
    32101 agaagctcga caaaatcctg tggctggaaa agaaaatgtg gcattatatg cttttgccaa
    32161 tgccacagat ttagatggtc agcttttacc tctggaagtg gcagctgttg aattattgaa
    32221 catcatccgt ccaacggttg ctctaactgt gtgggcggca ttgatgggac atgcactttt
    32281 cagtagacca gatttgtatc agcaattaaa aaatgacttt tcgacgttac aagatccgtt
    32341 tattcaagaa atgagacgct attatccttt cttcccaatg ctacctgcga tttcgctgaa
    32401 agaagtagaa gtagatggtt atcgtatacc agaaggcagt tgggttattt tagatttata
    32461 tggaacagac catgatgaac gaacagttga agcgccagat tcctttatga tcaaacggta
    32521 tgtagggaaa gcaaaagata tttcctataa agaagaatat gagatgattg cacaaggcgg
    32581 gggaaatttt cgacaaatgc atcgctgtgc aggagaatgg atcactcttc atagtctacg
    32641 tgtgttttcc gatcagctag taaataagtt tgagttctct gtacctgaac aagattggac
    32701 gattccattc aatcagtttc cgacttatcc gaatagccga gcgctacttt acaagaatta
    32761 gattagcata aacttatcga aaaaaaggaa gatcaaacag tgctgatctt ccttttttca
    32821 tattactata aattgataag gtcaattgat caaaatccac gaaatgcttc atcaatggtt
    32881 tgataatctt cattggataa atgcaaatct aaagcttgga cattgtcaga tacttgctct
    32941 ggacgcttag ctccaggtat aactacagac actcgtggat tcttcatgta ccaagctaaa
    33001 acaagttgcg caggagtggc ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg
    33061 gttaaaattt ctctgaaacg ttctccttgg aaatctggat ttctttttcg aggatctcct
    33121 tcaccaaatt gtttttcctc accaagttca tattttcctg tcagcaaacc ggaagcaaga
    33181 ggaaaatagg gaacaaaact gattttgttt ttttcaagat aagggaataa ttctttttca
    33241 gcctgccgat ggatcaaact gtatttatct tctactacat cgacatatcc atctgcattc
    33301 gcttctttca attgttctaa cgtgaagttt gacacaccga ctgctcggat ttttcctgct
    33361 tcttttaatt cgtgcaatgt agcaactgat tcattttttg gtgtgctttc atccggaaag
    33421 tggatataaa aaatgtcgat gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat
    33481 tgtttgagaa actcaggaga attatcaaat gttcgccctt tatttggtac atgagcagct
    33541 tttgtagcga tgacgacacg tgagcgatca tattcttttt ccttcagtac ctcgccaatc
    33601 agttcttcgg aacggccgtt tccgtaagca taagcggtat cgatcaaatt gatcccgcta
    33661 ttcaaagctg tacgaactac ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta
    33721 tgtcctccaa cggcatttgc tcctaatcca agctgttctg cgtagacttg tgaatgtccg
    33781 attcttattt ctgccatagc tgattcctcc tttttgtcta gtctaaaaca aaataccaaa
    33841 aagctgaact aaaacgtttt tgtgagggga aatagtcaga tatctcttga ctttgagact
    33901 ggtttttagt aggttataag taatatcgaa tgtcaatgaa agagcgagta agtttttgaa
    33961 actttacaag agaggatcat cgaggctgaa aatgatctaa agtttcatta gcctaaagac
    34021 actctggaga tggacgagtg aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa
    34081 gagaaatctt tcaaaattag gtggtaccgt gtatgaattt cattcaccct tgttcatgat
    34141 gaacaagggt gattttttta tttacatagc taagaaaggg agagaaaact atgagtttcc
    34201 aaagaccaaa aggaacaaat gatattttgc caggcgaatc agaaaaatgg cagtttgtcg
    34261 aagaaacagc acgtttatta ttcagagatt atcagtataa tgaaatgcgt acaccgattt
    34321 ttgaacacta tgaagtgata tcccgaagtg taggggatac aacggatatc gtttcaaaag
    34381 agatgtatga tttttatgac aaaggggaac gccatgtgac gcttcgacca gaaggtacag
    34441 caccaatcgt tcgttctttt gttgagcata aattgttcgg cccagagtat gcgaaaccgt
    34501 tcaaaacttt ttatatggga ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac
    34561 gtcaattcca tcaaatcgga gtagaagcat ttggatcaga aaaccctgca actgatgtgg
    34621 agagcatggt gatggcactt gatttcttca aacagttggg gatcacgcaa atccggctag
    34681 tcatcaattc attaggtgat aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact
    34741 atcttttacc ttttgaatca gagctgagtg aagactctaa acgccgcctt catgaaaatc
    34801 cattacgcgt attggacagt aaagacaaac gagatcaaaa atttgtggca gaagctccat
    34861 ctatcttaga ttatctaagc gagcctgctc aaaaacattt tgataccgtt gtttccatgc
    34921 tggatgcatt ggctgttcct tatgaaatcg acagtaatat ggttcgcgga ctagattatt
    34981 acacacatac gatttttgaa atcatgagtg acgcaccaaa aatgggggca caagcaacga
    35041 tttgcgcagg cggacgttat gacaatctag tggaagaact aggtggtcct tcaacacctg
    35101 gcttcggatt tgcaatgggt atcgagcgat tactcttgac tatggaggca gaggaagtag
    35161 ttatccctgc atttaatgag ttggatgctt acgtggtcgc actcggagac gaaacaaata
    35221 tcgaagcatt gaaagtggtc caagcgattc gaaactttgg tttttcagct gaccgcgact
    35281 tcatgaaccg taaagcaaaa gcgcaattta agacagcaga taaagcgaat gccaaattag
    35341 tattggtgat cggtgaagat gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa
    35401 gccgaaaaga aaaaagtttt ccattagaag agatatatga acattttgat gaagtctatg
    35461 atgaaatgac tttattcgag taggaggaaa aaaataaatg gcacaaagaa cgatctattg
    35521 cggattagtt tccgaacaac aattagaaca aacagttaca ttaaaaggat gggtacaaaa
    35581 aagacgagac cttgggggag taatctttat cgacctacgt gatcgtgaag gaatcgttca
    35641 aatcgtcttt aatcctgaaa tcaacaaaga agcatgggaa atcgcggata aatgtcgcag
    35701 tgaatacgtg atcgaagtga ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc
    35761 taaaatggcc actggtgaat tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc
    35821 aaaaaccccg ccatttcaaa ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa
    35881 atatcgttat ttggatctgc gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc
    35941 gacaacaaaa gcgattcgtc attatttaga tgataatgac tttctcgata tcgaaacacc
    36001 ttacctaggg aaatctacac cagaaggcgc aagagactac ctagttcctt cacgtgtcca
    36061 tgctggacat ttctatgcgt tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa
    36121 tgctggattc gaccgttatt atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg
    36181 agaccgtcag cctgaattta cgcaagtaga tattgaaaca acgttcttaa cagcagaaga
    36241 aatccaaact tacacagagg gattgattgc gaaggtaatg aaagaagttc gcggtatcga
    36301 agtcacattg ccatttcctc gtatgaccta tgatgaagca atggctcgct acggcagcga
    36361 caaaccggat acacgttttg atatggaatt gatcgatcta agcgatacag tcaaagaagt
    36421 agaattcaaa gtcttccaaa tggctcttga aaacggcggt gtcgtgaaag cattgaatgc
    36481 taaaggcgct gcagatcgct actcacgaaa agacatggat cagttaggtc aatatgtagg
    36541 acaattcgga gctaaggggc ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc
    36601 aattgctaaa ttcatgggag aagcaacaga agcaatcatt aaagcaacag atgcaaaacc
    36661 aggtgacttg ctgatgtttg gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc
    36721 tatccggaca cgtttaggaa aagaattagg actaattgat gaatcgaaat tcaatttctt
    36781 atgggtaact gattggccac aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc
    36841 tcaccatcca ttcacgatgc caaaagaaga agatatcccg ttattggctg aagaccctgc
    36901 aaaagtctat gcagaagctt atgatatcgt cttgaacggt tatgaattag gcggaggttc
    36961 tatccgtatt catactcgtg agcttcaaga aaaagtacta gaaactctag gctttacaaa
    37021 agaacaaatg gaagaacaat ttggtttctt gttaacagct ttagattacg gattccctcc
    37081 acatggcggt attgcattag gattggaccg ttttgcaatg ttattagccg gagaagataa
    37141 tattcgtgaa gtcattgcct ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc
    37201 accaagtatg gtttcgcctt tacaattgtt cgaattgaat attgatgtaa cgaatataga
    37261 agaatagccg ataaaaaggt aaaaaggatt tgtcgaatac gcttcttttg acaaccttga
    37321 tttaattgaa aaacacttta taatgaagcc ataacatata aagcggggct tttccatgaa
    37381 aaaaacattc gaaaattggg cttatcatga ttacacgacc aagctttcgg tatctatcgt
    37441 ttatgcgttg atcgcatcag tcgcattgaa tttcttttat cagccaggac atatttattc
    37501 aagtggtatt actggtctag ctcagatttt gacgactttg tcaaaatcac tgataggatt
    37561 cgaagtaccg gtatcaacaa ctttgtattt actgaatata cctttgtttg tattggcgtg
    37621 gttaaaaatc gggaagaagt ttacaatttt cacttttatc acagtcactt tgacctccct
    37681 ttgtatccat attttaccgg aaacagtcct atctaccgat ccaatcatct gtgcgttgtt
    37741 tgggggggct gtgatgggac taggtattgg gctagcgttg agaaacggcc tatcatcggg
    37801 cggtctagat atcgtgagta tcacgattcg taaaaaaaca ggaaagtcga ttggttctat
    37861 ttcgatctac ttcaatgcat tgatcgtttt tgttgccgga attctatttg gctggcaata
    37921 tatgttttac agtacgttgt ctattttcgt cagtggaaaa gtaactgatg tcgtttatac
    37981 taaacaaaag aagatgcagg taatgatcgt aacgaaacat ccagaaaaag tagtaaatga
    38041 aatccaaaac tgtatgcgta gagggatcac gatcatcaat gaagcagaag gtgcttataa
    38101 gcatgataag cagacagtat tgatgacgat cgttacacga tacgaactgc ctctacttag
    38161 agaagcaatg aaagcttctg atccacaagc ttttgtaagt attgcagaaa acgtccagat
    38221 acttggtcgt ttctatgagg atggcattta gatcgagttt gacaaaaatt catgaaatac
    38281 tgctgtagac agacgttcgt ttgtctgcag tttttttcta ccatagtaga atcagcaaag
    38341 aaaaactgct tttttatttg aaattttctt aaagttgaaa aaaagcatgt ttattttttt
    38401 aaatttatgg gatgatagaa aaaaatagta gagaaaggac acgattctac ttgctatttt
    38461 cacttaatac ccgtatatta cgaatgaaaa aagtttatga aaagttggaa tattgaatgg
    38521 aaaaacaaaa aaggttaaaa aacagccgct acatcactgg gtttgatggg ctccgttcat
    38581 tagctgtggt gggtgttatc ttctatcacc ttctgccaac tagtatgaaa ggcggctatt
    38641 tgggcgttcc gatttttttc gtcgtatctg gttatttgat cacggatctg ctgcggcagg
    38701 aatgggaaca aaatggaaaa atcaatattt ggcaatttta tgtcagaaga atgaagagac
    38761 tctatccggc acttgttttt atgcttgtga cagcttcggc ttatatcact ttgtttcagc
    38821 gagggctgtt aaacaatctt cgaggagttg tggtcagcag cttactgtat gtcaacaact
    38881 ggtggcaaat caataacggt ttatcttatt tcgatcgatt tgctaatgag tcaccgttta
    38941 cgcatatctg gtctttagca gtagaaggtc aaaactattt ggtttggccg atcttgtttg
    39001 tattgttgat ggtatttgtt cgtaaaagaa aatggatctt ttatactgtg ttaggtacat
    39061 ccttcctctc cgccatcttg atgatgatct tgtatacacc aggaggcgat ccaacccgtg
    39121 tttattacgg taccgacact cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg
    39181 tttggccaag tacgcgttta aagaaaaaca tcccgaaaca agcgaaaaat attctgaata
    39241 tcgctggagg aatctcgctt ttggcactga ttctttgctt cttcttttta gatgatcatt
    39301 acaatttcat ctattacgga ggaatgctcc tagtgagtct gttatgcgta gtgctagttg
    39361 cagtgacagc acaccccgga gcaagtttga atcgatggct gacaaatcca gtatttactt
    39421 ggattggcaa acgaagctat ggcatttatc tgtatcagtt tccagttatg attttttatg
    39481 aagcaaaggt gactgatatt gccgatcatg ttttgttgca tactttagtg gaaatcacat
    39541 tgattctaat catcagtgaa ctttcttatc gatatgtgga gcgtccgcta gcacgttttg
    39601 attatggtcg tacattccaa gtgatgaaag gatggttcag caaaccgatc atcagtaaga
    39661 gcaaaccatg ggttgttcca ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta
    39721 cggcaccgaa aaacgctgta acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa
    39781 gtaaaaaatt agcagaagaa accagaaaca attcagaaaa caccactagt tcggttgacc
    39841 aagcgatcct tgataagtac gagttgtcca aggcacaagg gaataaggca caaaaattag
    39901 aactaacggc atttggtgat tcagttatgt tggatgctgc aacggatcta caagaacttt
    39961 atccaaaagt agtggtagat ggagatgtcg gacgacaatt atatgccagt gtaccgtaca
    40021 ttgaaaaatt aaaggaacaa gatttgttga aagacactgt tctgattgga ctaggaacga
    40081 atggctcatt ctcggaagct cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag
    40141 tatactgggt caatgtccga gtaccgacga agcgctggca aaatgacgtg aatgcgatgc
    40201 ttgaacagat gacgaaaaaa tacgacaata tgacgctgat tgattggtac agctacagta
    40261 atgaccatag tgactggttt tatgatgatc aagtccatcc aaatcctgaa gggatgacac
    40321 attacattca cttagtcagc gaagccttgc taggaggaag ttcaagtacg aacagcagtg
    40381 agacaaacgt gacagaatga tagctacaaa aaacttcagg caaaaacatt tgtttttgcc
    40441 tgaagttttt ttatatttac cgtgttttta acattcatat agattcagga agcaaaacgg
    40501 ttgagtgtgt ctgtaaataa cgttcttttt gttttcgggt gagttttttg aatgcagctt
    40561 ctgcttttgt tgcttcgctt cttgaagaaa atacttctgc atggatcatc ttaacaggtc
    40621 ttcttttttg aagacgagta tatttagcac cagtaccatt gttatgttcc tctaatcgtc
    40681 tagaaagatc ggttgtatat ccaccataaa atgtctgatc tttacagaat aatacgtaaa
    40741 aataatgttc atttgccata gagcatctca cgaatctctg cagtgtattc tccatccata
    40801 ttgtaagtga tcaatggtgg agcgactttg aatccgtcta atttgccttc tttgattcct
    40861 tcaactaaca aaatatttgc ctcttttcca ggttttgggt aaacaaattg gatcctttta
    40921 ggtgcaattc gatagtcttc catgacctga atgatttcta agaaccgatc aggacgatgg
    40981 accatagtaa agcgtccatt tgtttttagt agcttacttg aaatatcgat cacttcttct
    41041 aacgtcgtat gaatctcatg acgggcgatt gccaggtgtt tattagggtt cgttttattt
    41101 gttggcagcc ctttgaaata aggtggatta cagacgacta agtcacaaga attatgtcga
    41161 acgacagata agctgtcttt cagatcgatg gtgtggacag tcatttgttc atttaaatgg
    41221 ttaagcgcga tactacgtct tgccatgtct gctagacgag gttgaagttc gatagcgtcg
    41281 atagccgcct gggttcgttt acttaaaaaa agtccaactg cgccatttcc tgcacaaagg
    41341 tcaacaatct tccctttttt aggtacacgg gaaaagtgag caagcagcac agcatctaaa
    41401 gaaaaagaaa acactttgga actttggata atcttgatat cttctgcgta taattggtcg
    41461 attcgttcgt cttcgtgaag catggattcc ctccaaaatg aaaaaaatag tcgtctgtat
    41521 tatactattt ttattaaaaa gaaccaaggt ggcttgtcaa acctctattt tttatgcata
    41581 ctatgaccat aagaagggaa aggaagagac tatgttttat cgatttatgc gcggggtagt
    41641 tcgagtacta ttagccttag ttaatggaaa tgcccgctat gagaataaag aagttttacc
    41701 gcaagatgag aattacatac tagttgctcc ccatcgtact tggtgggacc cattatatct
    41761 ggcggtagct gcagcaccca aggaattcag ctttatggca aaagaagaac tgttcaaaaa
    41821 tccagttctg cgatttatat taactcgatc caatgctttt ccagttaaaa gagaaaatcc
    41881 aggaccaagc gtcattaaaa cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat
    41941 catgtttccc agtgggacaa gacatgctac agaacttaaa ggcggaatgg cattgatttc
    42001 cagaatgtct aaagcgaaaa tcgtgccagc tgtatatcaa ggtccgttga cacttgggga
    42061 tctgttcaag cgtcagagag tgacagtgcg tttcggcgaa ccaatcgact tatcagatat
    42121 caagaaaacg gataaagaag gtatggcgga gatcgaacga cgaacacaag cagcttttga
    42181 caaattggac aaagaagtga accctgattt caaatatgaa gtcaaaaaaa aataaaatga
    42241 taagtttatg cgtgggcggt cttttgaatc tactgtcctg ttcagttcag actttttcaa
    42301 tgaaaacgag ggagagacgt caagtctgcc ctctttttgc gttccaatat gtaaaataga
    42361 agacaggtgt catgtaatgg atcaaagtgt tataataatt agaaggacat tttttgaggt
    42421 gattgaatgc gttttttaca tacagccgat tggcatatcg gaaaaaaact acaaggttat
    42481 gacttgattg aagaccagcg tcatgtacta tcttctatac tagatatcgc gaaagaagaa
    42541 aaagtagagg caatcgtgat tgccggagac ttatatgata ggagtgtccc agctgttgat
    42601 gccgtagaat tatacaatga attgatggtc gattggaatt taaaggaaaa gatcccgatt
    42661 tttgctatat caggaaatca cgatagcagt acgagacttt cagcgggtac gccatggttc
    42721 agttattcga actatttttt gcgaacacag ctagcagaag catttgaacc agtcgagttt
    42781 aaaaacgtac aatttttctt tcttccttac tttgaaccaa ttgcagcgag attatatttt
    42841 gaagatgaca atatccgaac gatcaagcaa gcggtagcca aggtgataga taaaatgcag
    42901 gagctatttg attcagaaaa aaaacaagta ttgattagcc atttttttgt aatgggaagt
    42961 gtaaaatctg attcggaaac gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa
    43021 ctacttacac cgtttgatta tgtagcacta ggacatttgc ataatcaagc tgctttgaga
    43081 caggataatg ctcgctatag cggttcccca ttgaaattct ctttatctga gatcaaccaa
    43141 aaaaaaggcg tatggatcgt tgacctgaca acaggcctgg agttgaattt tagggagatc
    43201 gaacccctgc atgatattga agaaataaca gcgagtttca aagaattgct ggatcctgcc
    43261 ttttatcagt ccgtcaatcg ggagaattac atctatatct atctcactga tagaacgatc
    43321 atcccgaata tgatgaacca gcttcggcaa atatatcctc gtattttggg gattgaacgg
    43381 gcaaacggtc gagcagaaca aacaaagcgc aaacgaaaag tactagaaat gaaagatcct
    43441 caaaaattag cagaagcatt ttttagtgaa atgacggaag agcagctaac tgcaaaccaa
    43501 gaaaaatggc tcggagaaac gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa
    43561 ccgaaaaaat tacaacttaa aaattttggt ccttttatca atgagaccgt agatttttca
    43621 cgtttgacag aagcaccgct ttttttgatc agcgggaaga caggagctgg caaaacgacg
    43681 atttttgatg ggatcacctt cgcacttttt ggtgaaactt ccggaagact tcgctctgga
    43741 aaagagatgc gttctttgtt tgctacacca gacgaagaaa caagcgtgac tttttctttt
    43801 gaacatcagc agatgagcta tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga
    43861 aaaggaaacg gaacaaaaaa acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa
    43921 ggaaaagaaa taaaacaaat cacaaaacgt acagaagcag atcagttgat cagagaactg
    43981 atgaatctcg atgcgaaaca gttttctcaa atcgttttac taccccaagg agaattccgt
    44041 aattttttgg tttcatcaag cagtgataaa gagactgttt tacgtcattt attcggtact
    44101 caatttttcc aacagttcaa tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa
    44161 ttggatcatt tagagcaaga gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa
    44221 gaggaaccag cagcagggtt tgaagccgtt ttagcccaat ggacaaacca tcaggcaata
    44281 ctcgaaaaac aaatcacgga agaaaaagaa gctctaacgg aacttcagga aaaacaaaag
    44341 caactagaaa attcctacta tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg
    44401 atattaataa acaagcaaaa ggaattagct gctcaaaaag acgaaataga ggaaaagaaa
    44461 cgatggattg ggtattatga atttacgcaa cgattagtcg aaccaatcca gcgcctacaa
    44521 gaaataaaag ttgaaaaaat agaactgtca caaaaaagta gtgagcaaac aaagaaacta
    44581 gcgatgacta cgcagaatta tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga
    44641 caaacaaaaa tcaaagaagc tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt
    44701 gtcgaatcct tacgtagtaa aaaacaagac gcagaacaat taacaatttc attggataaa
    44761 gaagaattga atgccgagaa gctgtatcaa aatctcgcta tctatcagga aagaactcga
    44821 attcttcaag aaaatttact ggatcaggaa aaactgcaag aagatcgtct tgcttttgga
    44881 cagttaaaat atctagatga aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact
    44941 gggtcgctcg cacagataag agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat
    45001 agtcagctat tgaaagaaca gacagaacaa gcagaacagc tgaaaagcca atgggcaaag
    45061 cagcaaattg ctcgtcttca gctttttttg cgtccagggg agccatgtct tgtttgtgga
    45121 tcaacggaac accccataca acaattaaaa cacgaacagc caaataagga agatatctta
    45181 aaagtagaac aacaactggc agaagctgag tctgctgtag aaagaactca gcaaatcatt
    45241 gccaaaagtg aagcacatga aaataatctt caacaacaat tatcagattt agaacaaaaa
    45301 atcatggcaa cgaatataca attggctgaa caagaagaac acttgcaaca ggctttgaaa
    45361 aaggcatttc ctgagataac agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa
    45421 tctttagata gacgaaaaag caagatggcc aatgcaagaa tagagctgga agaaataaga
    45481 caacaagcag aaaagctcga acaacaatta gctgtctaca aacagcagca gacagaaaaa
    45541 aataatcagc tccagcaact gcaaggagaa atcaaaagtt tagaacaaca attgcaagat
    45601 acggatagta actgtttaga agaggagata cgacgacttg aaaaacagtt agaagaagac
    45661 tcgaatcaac tagctgttga gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg
    45721 cttcggacac agcttgatct tcaacatgaa caaatagaaa aaatagatga aaaaatagaa
    45781 aaggaacaag aaagaatagc taaaaagtta gaaagccagt cgtatttttc aacaattgaa
    45841 gaaatctgtg catttgcaca acagcaagca caatataaag aaaaccaacg tacagttact
    45901 tcttatcatg aagagcaatt agtgacagct gaccggctgc gtcaattaga aaatatccag
    45961 ctaactgaga cattcccaga tacagaagag atcagccaac agttagaaga aataagacgt
    46021 atgatcgaaa taaagcaaga aaagatatac ggtttacaag aacaaaaaat tagtaatcaa
    46081 aaaatctacg atgaatgtct ttcgatttat cacacaagcc agacgaaatt agatgagctt
    46141 acgcaattac agcagctttc gcaaacattc aacggagaaa atcctaagaa gactagtttg
    46201 gaacggtatg tgcttcaggt ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc
    46261 caacgtttaa cgaaaaatcg ttatcaattt gagctagcgg ataccattgg cagttatcga
    46321 gggaaaaccg gattagaaat caatatctat gatgatcagg caggtatgtc tcgaagtgcg
    46381 cataccctgt ctggcggtga gagttttatt gcagcacttt ctttagcatt agctcttgcc
    46441 gatgtgatcc agacacaagc tggtggtgta gcaatcgatg cattgtttat tgatgaagga
    46501 tttgggtcat tagatgaaga agctttggaa atggcaatgg aagctttaga aacgatcgaa
    46561 agtgaaggaa gaatgatcgg catcattagt catgtaagag aattgaaaga acgagtatta
    46621 caacaaataa gaattgatac agaaggtagc ggacaaagca aaatacgcta tcaacttgga
    46681 taaaaaggga gattggtaac tatgaaatgg agtattccag aaaaagtcat tgcacgtgga
    46741 cgtcaatact tgcaagaagg ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg
    46801 tggcatgcag aagtcttggg cagtgaattg tatatggttg atttggatgg tacagcaaaa
    46861 gagatagatt actgccaatg tccctattgg gaggaacata aatattgtaa gcatacagtt
    46921 gctgtggaac tttatttacg gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca
    46981 atgctagaac cggtaaaagc aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt
    47041 ctaaaaacac aaaacaatcc tcaagcggtg caacctttga ttattgaata ccaagtagaa
    47101 acgattgaga ctaatcagta tcacccagag ctttctattt tggcacttta cttgcgtatc
    47161 ggttcattgg catcaccgaa aaaaacatat atcgtaaaaa acatctatga ttttttacaa
    47221 gcttacacag aaaaagaaac ttacactgtt aataaacagt accagtttcg tctagataaa
    47281 cgggcgtttc aaaaagaaga tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg
    47341 catcaattat taggtacgaa cggcttacaa gtcaaaggaa aactagataa aagatatctg
    47401 cttctcccag tagaacaagc ccgcttcctg ttagagcaga tgaatcagac tacacgtttg
    47461 catctgcaga taaatgaaca aaacgatcaa ggaatacaat tttccgatca aaacaaacct
    47521 ttatcattca aagtggaaaa atttggagaa gattatttgc tgcaagcatt gaatgatttt
    47581 gattttttct ttatgcatta tcagtgggga gttatccaag ggacatttta ttctctgacg
    47641 aaatatcaac aaacggttta tcaaacttgg aaacaattga ttcgccgatt ggagaaacca
    47701 gaagtagtgt tcaagaaaaa agaattaccg tctttgttta aagagattat ccctctgctg
    47761 tcagagatcg gcgacgtagc tattgcccct gcagtagaag aggaagtagc agatatcccc
    47821 gtcgaattta ttttcttttt ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc
    47881 gtctatggtg aagtcatcta ttcgactgac ccgaagcatg aggtctcgac agaacaggct
    47941 tttcaggtgt tacgtgacac agtagaagag aaacgagtac ttgatctatt cagaatgtat
    48001 cgctaccaga aaaatgaaac tggttatgaa cgtccattgc caagcgggga agaactatat
    48061 gcctttttcc gcacagaact atctgtcttc cgtcagtttg gtgaagtcag acttggaaga
    48121 aaattgcgag agttgtattt agatgcgcat aaacatcaac cgaaaatcga agtaaccgag
    48181 accggttcat ggctcgatat tcgattcgat gtgacaggga tagaggagca agaaatcgat
    48241 catgtcctgc aaagcttgct gagaaatgat gctttttata cattagaaaa tggtcaggtt
    48301 ctttcttttg attcagaaga atttcaacaa acaagccaag tgttgcaaca attacgggaa
    48361 tcgatacgta cagaagaagg aacgatccat gtacctaaaa atcaaggatt gatcattcaa
    48421 aatcaattgg aaaaaagcaa tgctactttt agtgaatcgt tccaaacgat ggtacaagat
    48481 ctcattcatc cagaaagata ccaggcacag cttcccaaag gattgaatgc aacgatgaga
    48541 gattatcaaa agcaaggttt tcgatggttg aagatgttag gtcattatca gtttggcggt
    48601 attttagccg atgaaatggg acttgggaaa acactacaga caattgcctt tttactttcg
    48661 gaaaaagaag aacggaaaag cttctctgct ttgatcgtag cgcctgcgag cttaatttac
    48721 aactggcagg cagaggtgag aaaattcgcc ccttctttaa gtatccaagt aatcaatggg
    48781 aataaaaaag aacgtgaaga attactggct aaagacacag atatccgagt gacttcttat
    48841 gccagcttaa gacaggatct agctaattat cagtcgcaaa aaattgatta tttgattctt
    48901 gatgaggcac aaatggtcaa aaatagcagt acgaaaactg cacaagcatt gagagaatta
    48961 gctgtacctc agcggtttgc actgagtggg acaccaatcg agaataattt agaagaattg
    49021 tggtcattgt ttgcaacgat tatgccagga tttttcccga ctaaaacaaa attcagggaa
    49081 cttgccgcag aagaaattgc gcaaatgatt cgaccgttta ttttacggcg agataaacaa
    49141 actgtgttga aagacttgcc ggaaaaaaca gagatgaatc tatattctgc acttacagaa
    49201 gaacaaaaaa ctgtttattt agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg
    49261 gattctgaag cattcaagaa aaaccggatc gggatacttg ctggtctgac aagactgcga
    49321 caaatttgct gcgatcctcg tctgtttatt gaagattacc aaggcggttc cggcaagctc
    49381 gaacaagtaa aagatctact ggtagctgcc aaagagaaca aacgaagagt tttgctgttt
    49441 tctcaattta cagggatgct gactatcttg caagaagagt tagccgagct agggatctcg
    49501 acattttatt taagaggcag tacgaaacca caagatcggt taagtatggt agacgcattt
    49561 aatgctggcg aaaaagatgt ttttctgatt tccttaaagg ctgggggaac tggattgaat
    49621 ctcacgggag cagatacggt catcttgtat gacctttggt ggaatcctgc cattgaagaa
    49681 caagctgctg gacgtgcaca tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg
    49741 atctctgaag gaacgattga ggaacgcatg gattcactcc agcaagaaaa acgcgaactg
    49801 ttccaaaaag tgatccaagg taatgaagaa cagttgacga aactgactga agaagacatc
    49861 cgcatgattt taagtgtagg agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa
    49921 tgggtaagaa agcctttcta aagaagcgat tgtttatttg atttaaaaaa tagcaaggag
    49981 gctgggattc cattttatcc cagcttcctt gctatcattt ttttatttct atttttaaag
    50041 gttatacaaa gattgtcttc attgtctcta acagctgatc aaaagaagaa atctcgatat
    50101 cgcaaattgg agtctgttca attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt
    50161 tagcactttt tgcacctaga acatcattgt cgtaattatc gccaacgtat aaggtctctt
    50221 ctggaagcat atgaaattct ttctcagcca gctgaaaaat ctctttttct ggtttttgga
    50281 aacctgttgc ttgagaaatg agcatattgc gggaaggaat ccaattgttc aattgaagtt
    50341 gcttcagttt ttttgtctga tggtcagtcg gcccatttgt gataattcct aatgggacat
    50401 tcttttcctt caagaaatcg agggtttttt tgacctcctc atgcaagcag atattatcaa
    50461 gttcttcctc gtaaattttt tgaaataaga ggccatcttc ttccgtaata tgagggtagt
    50521 ctaaatcttt tagtgattgg ctgatccgat gtgctcgcat atattctagt gtccaatctc
    50581 ctgccattac ttttgggaag ttttcatcac tatgatggcg gaaccggata tatagtggat
    50641 gcatatctgc atctgaaaca agcggaacga ctcttttgac cgcgtttcga aatggctgct
    50701 gctgatcata catcgtatca tcaacatcaa acacaactga tttaatcaaa attttacacg
    50761 cctttcggtt ttcattgatt ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc
    50821 taaaaaagct gataaataaa ggtttttctt actatatttc tatttccttg acagaataag
    50881 gtttttatat ttcagttttt ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa
    50941 caaattaatc attatggtag aagtcgattg atttatttta atgcaatctc tgtggaattt
    51001 tcgaaagatt tattttttta atacagtctc aatataacta ttcaa
//
LOCUS       DACCWK010000007        50650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCWK010000007 DACCWK010000000
VERSION     DACCWK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.007"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            233..2377
                     /locus_tag="IVW65_000375"
     CDS             233..2377
                     /locus_tag="IVW65_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7899302.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2429..3470)
                     /locus_tag="IVW65_000376"
                     /pseudo
     CDS             complement(2429..3470)
                     /locus_tag="IVW65_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(3747..5549)
                     /locus_tag="IVW65_000377"
     CDS             complement(3747..5549)
                     /locus_tag="IVW65_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899303.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(5563..6315)
                     /locus_tag="IVW65_000378"
     CDS             complement(5563..6315)
                     /locus_tag="IVW65_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899304.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(6324..6686)
                     /locus_tag="IVW65_000379"
     CDS             complement(6324..6686)
                     /locus_tag="IVW65_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7899305.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(6862..7734)
                     /locus_tag="IVW65_000380"
     CDS             complement(6862..7734)
                     /locus_tag="IVW65_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7899306.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(7785..8273)
                     /locus_tag="IVW65_000381"
     CDS             complement(7785..8273)
                     /locus_tag="IVW65_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7899307.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(8411..9202)
                     /locus_tag="IVW65_000382"
     CDS             complement(8411..9202)
                     /locus_tag="IVW65_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7899308.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(9257..9865)
                     /locus_tag="IVW65_000383"
     CDS             complement(9257..9865)
                     /locus_tag="IVW65_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7899309.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(9858..10175)
                     /locus_tag="IVW65_000384"
     CDS             complement(9858..10175)
                     /locus_tag="IVW65_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7899310.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(10331..11059)
                     /locus_tag="IVW65_000385"
     CDS             complement(10331..11059)
                     /locus_tag="IVW65_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7899311.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            11218..12099
                     /locus_tag="IVW65_000386"
     CDS             11218..12099
                     /locus_tag="IVW65_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7899312.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(12336..13379)
                     /locus_tag="IVW65_000387"
     CDS             complement(12336..13379)
                     /locus_tag="IVW65_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7899313.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13382..14050)
                     /locus_tag="IVW65_000388"
     CDS             complement(13382..14050)
                     /locus_tag="IVW65_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7899314.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            14192..16528
                     /locus_tag="IVW65_000389"
     CDS             14192..16528
                     /locus_tag="IVW65_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7899315.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(16643..16954)
                     /locus_tag="IVW65_000390"
     CDS             complement(16643..16954)
                     /locus_tag="IVW65_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899316.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            17135..18610
                     /locus_tag="IVW65_000391"
     CDS             17135..18610
                     /locus_tag="IVW65_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7899317.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            18657..19241
                     /locus_tag="IVW65_000392"
     CDS             18657..19241
                     /locus_tag="IVW65_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7899318.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            19243..19662
                     /locus_tag="IVW65_000393"
     CDS             19243..19662
                     /locus_tag="IVW65_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7899319.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(19943..20227)
                     /locus_tag="IVW65_000394"
     CDS             complement(19943..20227)
                     /locus_tag="IVW65_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7899320.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(20459..21169)
                     /locus_tag="IVW65_000395"
     CDS             complement(20459..21169)
                     /locus_tag="IVW65_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7899321.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(21166..22638)
                     /locus_tag="IVW65_000396"
     CDS             complement(21166..22638)
                     /locus_tag="IVW65_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7899322.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            22776..23249
                     /locus_tag="IVW65_000397"
     CDS             22776..23249
                     /locus_tag="IVW65_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7899323.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(23402..23953)
                     /locus_tag="IVW65_000398"
     CDS             complement(23402..23953)
                     /locus_tag="IVW65_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7899324.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            24058..24588
                     /locus_tag="IVW65_000399"
     CDS             24058..24588
                     /locus_tag="IVW65_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7899325.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(25071..25514)
                     /locus_tag="IVW65_000400"
     CDS             complement(25071..25514)
                     /locus_tag="IVW65_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7899326.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(25530..26690)
                     /locus_tag="IVW65_000401"
     CDS             complement(25530..26690)
                     /locus_tag="IVW65_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7899327.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            27197..28897
                     /locus_tag="IVW65_000402"
     CDS             27197..28897
                     /locus_tag="IVW65_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899328.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(28999..29832)
                     /locus_tag="IVW65_000403"
     CDS             complement(28999..29832)
                     /locus_tag="IVW65_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7899329.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(30136..32301)
                     /locus_tag="IVW65_000404"
     CDS             complement(30136..32301)
                     /locus_tag="IVW65_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7899330.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            32561..34855
                     /locus_tag="IVW65_000405"
     CDS             32561..34855
                     /locus_tag="IVW65_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7899331.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            34848..35513
                     /gene="pgmB"
                     /locus_tag="IVW65_000406"
     CDS             34848..35513
                     /gene="pgmB"
                     /locus_tag="IVW65_000406"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7899332.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            35506..36513
                     /locus_tag="IVW65_000407"
     CDS             35506..36513
                     /locus_tag="IVW65_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7899333.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            36535..37554
                     /locus_tag="IVW65_000408"
     CDS             36535..37554
                     /locus_tag="IVW65_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7899334.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(37835..39202)
                     /gene="nhaC"
                     /locus_tag="IVW65_000409"
     CDS             complement(37835..39202)
                     /gene="nhaC"
                     /locus_tag="IVW65_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7899335.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(39311..40732)
                     /locus_tag="IVW65_000410"
     CDS             complement(39311..40732)
                     /locus_tag="IVW65_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7899336.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(40975..42852)
                     /gene="tdc"
                     /locus_tag="IVW65_000411"
     CDS             complement(40975..42852)
                     /gene="tdc"
                     /locus_tag="IVW65_000411"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7899337.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(43143..44399)
                     /locus_tag="IVW65_000412"
     CDS             complement(43143..44399)
                     /locus_tag="IVW65_000412"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7899338.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            44979..46280
                     /locus_tag="IVW65_000413"
     CDS             44979..46280
                     /locus_tag="IVW65_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7899339.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(46349..46948)
                     /locus_tag="IVW65_000414"
     CDS             complement(46349..46948)
                     /locus_tag="IVW65_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7899340.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            47202..48083
                     /locus_tag="IVW65_000415"
     CDS             47202..48083
                     /locus_tag="IVW65_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899341.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            48096..49706
                     /locus_tag="IVW65_000416"
     CDS             48096..49706
                     /locus_tag="IVW65_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7899342.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWKPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(49783..50442)
                     /locus_tag="IVW65_000417"
     CDS             complement(49783..50442)
                     /locus_tag="IVW65_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7899343.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT        15444 a        10395 c         9059 g        15752 t
ORIGIN      
        1 ccatttttat ccccacttgt ttatccccaa acaaaggtgg tctatgcaat aaaatgaacg
       61 tcaaagaaaa acgaatgaat gctcgttgct tatttacaaa ttgttttatt tagaatacac
      121 agacagattt atgaaaagaa aaagcttttc gacacgatta tctgttccaa aaaatgtcta
      181 atgaatgctg aataagacag aaaatcaatg aaaggaaaag gactagttta ttatgaaaaa
      241 taaaaaaacg atacgtttgc tagctgctac ttggctgatt gctccgtctg ttttgtcgac
      301 caataccgta tttgccgtag ataatcaaat agctgatcaa acagtcacaa cagatacgac
      361 tacatccagc tcagctacta gtgaaacaac ggattctttt acagaagacc caactggaac
      421 aactaactca acaacaggat cgaccgagcc aactgaaatt gatccggctg atccatcaga
      481 aaatgaaaag ccagccgaga catattccct tacaattgct ccttctttga ttggtaagat
      541 ccaactgact gcttcgacag cggattcccc tgaacagcaa cctttggata ttacggaaag
      601 tggaacggta gaaggtttat tagcggaaac gaaggtaact tacactatta cgccagctga
      661 aggtgaacaa attgcttcag tcgctgttaa tggtgccaaa gaagtaaatt atgatgcaaa
      721 gacctttaca gtaggtacga acaatttatc tattactgca gaatttaaag ctggaccgga
      781 tacttcaaca gagaaaccaa ctgatcctag tgaagaagtc cctagtacac cagatccgga
      841 aacgccaaac aaaccagatg tgggtaataa caacaataat aatggttcaa ataattcaaa
      901 caataataac aataattcga acagcgggaa ttcttcgaat caaacaaacc catctggatc
      961 gaataacaat ggaaatactc agagagaacc aaaccatcca aacaatggtt catcgaatgg
     1021 cggaacgaac aatgcttcat ctcgtccaaa tacagcaaat caaagcggta tagagaatcc
     1081 atcttctgct tcttctgatt ttgttgtaaa atcaccaatt gatgctgtat tgcctacaaa
     1141 tacaacgaat gtccaacaag caatcgttcg agaagcattc aagcatcttg gaaaaccata
     1201 tgtatgggga gcaaaaggtc caaatacttt tgactgttct ggtttgactt attacgtata
     1261 tatgaaagca accggtcatt atatcggtgg ctggacaggc gaacagcaat atgcaggtac
     1321 gcagattcca gtaagtcagg cccaacctgg cgacctcgtc ttctggggac cttcttccgg
     1381 tgttactcac catgtaggta tctatattgg gaacggacaa tttatccatg cgcctcaacc
     1441 aggagataaa gttcgagtaa cttctatttc agatttcacg cctgattttg ctgtccgagt
     1501 aaacttagca ggtttacctg ctgcttcagg ttcgctgaca ggcggctcat ctattttaga
     1561 tggattagat ggctcgttcc atttcagtca aaaccaaaca actgatcagt tcttgaaaaa
     1621 gattgcagat gatgcacagg aaatcgggca aaaagaagga atctacgcat cagtcatgat
     1681 ggctcaagct atccttgaaa gtggatctgg aaacagctta ttatcaagcg aaccaaatca
     1741 taaccttttt ggtatcaaag gatcatataa aggaagcagc gtgaccttca atacacttga
     1801 acaagatagt tccggacaaa gctaccaaat ccgcgcacaa ttccgtaaat atccttctta
     1861 caaggaaagt ttggaagact atgccgactt gatcaaaaat ggactgactg gcaatcctga
     1921 tttctacaaa ccaacttgga agtcggaaac aaaagactac aaagaagcaa ccaaatattt
     1981 ggaaggacgt tatgctacag atcgccaata ttcacaaaaa ttgaacgcga tcatcgaagc
     2041 gtacgacctg acaaaatatg atgaaccgaa aaaagaagca gcaacaactg aagaagcaaa
     2101 cgctgaaatc ctcaatatgt ctaagcgatt cgatgtgcca atcaaatgga caacagatgg
     2161 cgtgccaatc acaggattag aattttcaac gataaaacgc gtcaacaacg cttcttccat
     2221 cttccgtgtc gcttcaatct gggatttgtg gaacaatttt acggcaagac agataccaga
     2281 aacaactact cgtacagtta aagctgcaac tgttcctgta ctaaccttgt taagtttaga
     2341 caaatcattc tggcttgatc tgcctttaaa gaaatagaat aaattaaaaa ggggatgtga
     2401 cactgtcaca ctcccttttt aatttattct atttctttat atttattttg tattccgtta
     2461 caaactcgcg tacttggtta ggttccagag tgacatctcc aaaactttca agtcgctccg
     2521 ctcctggcag ttcttgagtc tcaaatgtga taccgccatg atttactaat ttattcccgc
     2581 gcatttccgg tccatcttct ccaaaatttg ctgtaaaaat cacgattcct gaagcatctg
     2641 tttttacttg gatcgatact ttttcatctg ggcttgtcaa ttccgcagca tttagtaaac
     2701 cagattgttt tttcaacaaa aatggatgat cgatgccgcc cactatcatt gattggcgaa
     2761 aatcactggc aaagacctca cctaattttt taggacgttg gaagtcaaat gctgtgccac
     2821 ttactgaaac tacttctcct gtaggcgttg tatctttatt gagcgtagca aataatcggc
     2881 tattcaacca aagtatatgt tcgtcgattg actgtgctgg atcaccggtt aaattgaaat
     2941 agacatgatt tgtaggattg aatactgttt tgcggtcact gatcccttgg accgtcactc
     3001 gccatgtatc atcgttcgtc agcgtatacc gtacttctac atctaaagtt ccaggaaatc
     3061 cgttttccat atctgggcta gtctgataga aaatgaccga tgcttcgttt tcaccattga
     3121 tgactgtata atgccactgc ttggcttcaa agctaggtgc accaccatgt aaaccaatga
     3181 ccagtgtccg ggtctgtttt tacttcgaac gtttcatcat ctaatttgaa tgtgccattc
     3241 gtaatccgtc cagccatacg tccgattgaa gcaccgatat aagggtcttt ttctagatat
     3301 tcttttgctt catcaaaacc taagatcaac tctcgttttt cattatcgat tattgtttga
     3361 aaatgaacaa cccgtgcccc aagatcactg acagacaaag atgtcccatt ttgattcgtg
     3421 atcgtaatca agtgatagtt ttcgccaaaa ttttgttcat tgatttccat gttccttgca
     3481 ccctcttttc tttcatttgc actgattcca gcatacatgg aaaccgtttt cttaacagct
     3541 tattccgtga gtaacgagaa aaaatttact atattctaat aaaagaccgt gaaaaaaagg
     3601 ttgtgacaaa aatattgtca caacctcttt ttccgaacaa acggtgttca aaagcttgca
     3661 tctgcggtaa taagcccaac caaacgaaaa atatgaggag ctatttttgc ttggctgttc
     3721 ttattacttg atgcaggaca gctttttcac aatctctttt tcaatacgat tccagtatat
     3781 aaccatgtcg tcagtacata gtagatgaaa tacaatacga agaatatcgt aaacggaacc
     3841 caaatatctt tgtatggatt ttgcatgaat agaccaaaca tttgtaatcc gaataagaca
     3901 tggactacac ctaagattcc tggtgctaag aataacacgc ctacttctct acggatcgat
     3961 tgtttaagca acccgcgtcg tgttcctatc ttttcaagca tttcataacg agcaatgtca
     4021 ctcttgcttc ctgacaagat cttgaacatc aaacagctag caagcatggt caagaaagct
     4081 aaacctagga agaatcccat aaattcaaaa ccagagaata gaccattata cacttcatac
     4141 atttcatatt tctggttgct gtactctact ttttgcaaag atggattgtt cattttattt
     4201 tgagcaacaa gtgtttcgat tggtttcagg tttgcttgga agtcttttac ctgaactagc
     4261 tgtaaagtgg attgtggcaa gttcagttga tcaaattctg attgactgac taccttgatt
     4321 tctttttctt gctgttccgg caattcatag cttcgaagtt cctcttgtac agtcagttca
     4381 ttcatttttt tagcaggaac tttttctttc gtgatcttcg gcggtgttcc atcttcatgt
     4441 tttatcatga tcaatggttg agccgcaaat tcacttgcat tatagtaaat cgttcccgca
     4501 tcttcttttt gagtataaga agcagaaagt gttggtgaaa gttctttgat tttttgctga
     4561 tcttctgccc gaggattatt catcactaga tcatatccag aattgttttc tgtcaagtct
     4621 gcaatttcat ttttaaaacc taatcctacc gtcaaagcgc ccagtgccaa ggcaaacagc
     4681 atagcgacca ttgagagcat ttgtgtatat tcacgaatac gaaagtttaa ctgtgacaat
     4741 gtgaaattgt tcaatttttt caatgaaatc tgttctgatt tctttagtaa tgataaaaag
     4801 aaaatgatca cagaatggaa aatgaaataa gttcctaata cgatcgtaac taaggctacc
     4861 cctataccaa agacttgata ggtccccacg tgtttcatca tatagtaacc gcagcctaga
     4921 aaagcaattc ccagtactac ttcaaggaaa aacaagaatt tatgctgctt gattcttgtt
     4981 ggtgtctgat ccgcacgaat caatgctaaa atcggttttt tgacaatcga aaaagcattg
     5041 acaacagcag ctaataaaaa caaaacagca aaaaacaaaa cagtgatcaa caacccgtga
     5101 atactaaaag gtgtaaaatg cgaaatattg agatctaact gatccactaa taatcggtgg
     5161 acgatacttg tcaatcctac accgacagct gaacccacta aagtagctgc gatccctact
     5221 aagaatgttt cagaaaaaat catctgtgcg atttttctgc cttttgctcc cagcatcata
     5281 aacattgcat agtctttttg ccgcatcgtc atcaaaaagg aatttgcata taaaatataa
     5341 acgaatgtga tgatccctag taaaacagaa ccgaatcgga aaatgataac tgtagccgca
     5401 acgatcgtat tacttttcaa aaaagcttcg ttgcttgcca ttgattcaaa catgtaaaaa
     5461 atagcagatg ccataactaa tcctgagaat aacaccaagt agtctcttaa acggctcttg
     5521 atcccagtaa gtgataattt ccaaagcata gtcaatctcc ccctactgtt ccaagtttcc
     5581 aagaatagat agtatttctc gatagaagga atcacggctt tgatctccac gtttcacttc
     5641 ttgatggata ccgccatcct tgatgaataa aatacgttgg caataacttg cagaaaacgg
     5701 atcatgcgtc acaagaagaa tcgatacatg atcttttgta ttcaattcgt ccatcgtatc
     5761 taaaagatcc cgggcacttt tggaatccag cgcacctgta ggctcatccc ctaatagaat
     5821 cgttggttgt gtgatcaatg cacgtgcggc ggcgacccgt tgtttttgtc caccggaaat
     5881 ctcagaagga tagctttcca aaatatgaga gatggataaa cgttctgcaa ttttttggac
     5941 acgttgcttg atttcttttg gtctgacgcc ttgtaaagaa agaggaacag cgatattttc
     6001 ttgtgctgtc aagctttcta ataaattgaa atcttggaaa ataaagccaa tctcattcgc
     6061 acgaaaatct gctagttgat ttccttttaa tgtggtcaca tctttttgat tgattcggat
     6121 atgcccatct gttggtttat ctaatgtgga taaaatattc aaaagtgtcg acttaccaga
     6181 accagaagcg cccatgatcc caataaattc gcctttttct acatcgaagc tgatgccgct
     6241 taatgcttgc gtctttttct cattgttttt tccgtatact tttgttacat tctgtacttc
     6301 gactattttg ttcatttctt tccctaccct ttaattagtt tttccagtgc tgttttcggc
     6361 acgtcttttt tttcgacttc ttcatacgaa gtgacacctt tatttttgtt ccaggtgatt
     6421 tttaaatatg ctcctttacg aagcggtcga gtcaattgtc ccttgaagtc cagttctttt
     6481 tccttaccct ctttcgtata gccaggcagg atatatctat agttgactgt ttcttgtcca
     6541 gagtctgatt ttgaaactgt ttttgtacca tctgtcgtaa tttgtacata gtattcttct
     6601 ccacccataa ctacgtaatc tgcgattttc acccctgcaa gtaccacaac gatgaaaaca
     6661 agtagaccga tcagaagttt tttcactttt tccccttctc tcttacttta tatactgatt
     6721 atagaaaaaa gcgaacaatt ctgccttcga atcaactatc attcttcttt caattttgtc
     6781 attttcttta aaacaactaa aaaaagagtt caagacaata atccgtccta aactccttct
     6841 tcctatctta caaaaagtat gtcacttacg gtctttcatg tcgcctgtat tgataacagg
     6901 ttgcgttcct ttgtctgtaa tgattgagac aagtaaatta ttgatcaatt ggactttccg
     6961 atcatcggtg aaattgattt cttgtccatc ttcgatttgc tctaaggcca tttgagtgat
     7021 tgtgacagct ccttcaacaa tggtttgtcg agcagacaaa atggcttttg cttgttgacg
     7081 ttggagcatg gcactagcga tttctgttgc ataggctaaa tggttcaagc gtgtttcgat
     7141 tacttcgaca cctgctactg ctaaacgctc ttgcagctct ttttgcaact cgtcagacac
     7201 tgccgtcgtg tttcctcgaa gcgtcaaatc attctcattg aatgtatcat acgggtattg
     7261 gctagcgata tgccgaatgg ctgtctcact ttggatttct acaaaatctt gataatacgc
     7321 tacatcgaat aatgcctttg ctgtatcaac gactttaaag actactactg ctgagatttc
     7381 gatggggttt ccatccaaat cattgacttt taaaacagaa ctattgaaat tccgaacttt
     7441 caacgaaaca gtcatttttt gtgccaaagg tatcgtaagg aaaaagccat tttctcgaat
     7501 cgtacctaaa tattgtccaa aaaacaaaat tacttttgct tgattaggac tgactacagt
     7561 tgccgaactt aacaacaata acgtgatcaa ccaaagaaaa atacttaaaa aaagtgccca
     7621 aactttcgct tgccacatac ctaaatagaa gaaaaacaat ccaactaaag ctaatacgac
     7681 caatccgaca atccctgcat aaccattgat ataaaatgct cttttttctc tcatgctact
     7741 tcctgcgctc ctttatcctt caataaatat ctaatgttac ttttttaatt tttttttcga
     7801 atacgcgcca ttacaccttc tagttctaac tctttgtctt cagtgatgta ccaaccgctt
     7861 gcctgtgtcc ctccggctgc ctgcggtaaa taatccaatt ttgttcgaac ccagtgcttt
     7921 tcttctgtaa aatagtcttc taccaagacc tctagatcca tccctgctga aagaggcaaa
     7981 tcttcccgaa cagatttaaa gataaatcgt tccttcttcc catcatactt caatattcct
     8041 gtatcgataa tcggtaaaga ataataatcc agctctttct tcaccgtcaa taaatgtttg
     8101 aaaacttcat ggtacacttg ctctaattgg cgaccttctg aattgttcgg gtaattaacc
     8161 atcacttttt ctttgtctgg atcatagaag gtttggctta gttgagtgat ataagtatgt
     8221 aatctttcca tttctgtttt taacttttct ctttgacctt cttctacctt catatactct
     8281 ttcaccctgt tttcttttta gatattttct ttcttatttt actatagtta tgtgcgttaa
     8341 gcgatgaaaa acagcaaaga ttggaaggat ctttgctgtt ttttcttaaa atatccagtt
     8401 gagtacagga tcaatctgtt tcattgtcct cttgatccgt cgtaatattg atcgatgtgt
     8461 ctttagcagt gatctttagc tttttctgtc cttgttctaa caaagtattt gttattggct
     8521 ggtagtcttt ttcaatataa gatttaccag acgtttggat agtatagcca ctgttttcca
     8581 tctcatgcaa agataaattg ccaccgttta ggatgacttt tccttctttg ctgatcaaac
     8641 tgtcttctag atggaagttg ctgttttcac ctgtgacgat caagttatcc acattggcct
     8701 catctaatcg aagtgagcct aattcatctt tcgcagttat tgttcctcct gtaaattcat
     8761 tgaacgatac ccagccatca tgtgtcttag tattcacttt tttgattgat acttcgttga
     8821 tatcaattgc actattattc ccttcgattg tcaattcaga taattcatga tctgcaggga
     8881 caacaatagt gatttgtgca cttgtgtctt ctaaactaat gattgcacct gttgattttt
     8941 tgactgcatt cacagtcaac acgccatttt tctcctttat ttctggcttt tctgtcttat
     9001 cataagtcgt ttttatctca aaatccaatc cgcgtatgac cgaaactgtt tgattttctg
     9061 catggatgac gattttgttt gcatccttga ttttttttgt ctcagtaacc atatcgatca
     9121 accgaggacc attatcccaa gtcagactct tttgagcgcc taatgtataa gttgcacctg
     9181 ctaggaggcc accagtaagc atcagaccaa taccggtaat caaaaaatgt ttcaatctca
     9241 atttatgtgt cctccttcaa acgcccgctg aatgcagatc tctttttgta ataaaacgtc
     9301 gtcccatcca tttaacaaag ttcatcatga cataaaaaag cccttttgtt cctttcagca
     9361 acagtggcga aataaatact gcgcttcctg tggcgaacag accgacaccg ataaagaata
     9421 atccactcag aatcgattgt ccaaacacac tgattcctga gatgatcatg aataggccaa
     9481 cacctaagat actgatacat aaaacataga tactaaaaat gagcgctagt aaagcaacca
     9541 atagtccccc aataaccaat ataaacccca gtgctagtgg aatgaggaca ggagaagcaa
     9601 ataaaccgat aatgattaac caaatcaggc gaatttgtct ttttggattt ttcactaact
     9661 cgttttctgc ttgttcttct tctgaaaagt aactgatttt taattttaag gcaaactgct
     9721 ttggtgtacc atattcatca atgatctgat ctaatgtttt tccagaatct aaaaaatatt
     9781 cctcataata ctggatcaaa tcaaaacgat cgtcttcagg aatttcttga agatgatcca
     9841 caacttcatt caaatattca ttcatttgtg ctctcctcca ataaccggtc taaagaatct
     9901 cgaaaaacaa tccattcatc aataatttct tctaaatgac gacttcccat tggtgtcaac
     9961 gagtaatatc gacggtttct gccttcaaaa ggttcatcat aggtctcaag atatccttct
    10021 tttttcaatc ttctcaaaac cggatatagt gtagattcac tgacagagag actttcttgt
    10081 acgatttttg ttaaggcata cccatacata tcttccttgc ttaatgctgc tagcacagta
    10141 ccatcaagaa gaacagttgg aatctggatg tgcattacgt caccttctat tctcaagtgt
    10201 aatattttat ttcaatctat attatacaac atataatata gtttcaaaat cagtttgtca
    10261 aatacaaaaa aactgactaa aagatttgat cctttttagt cagcaaaaaa tggcttctct
    10321 ttgattttta ttataacagt ggcataatcc aatttgtcca catccagctc agtggcatga
    10381 taacgatcat tgcaggtatc atgtcagcta ctgggaacat tttcacgttg atcattctaa
    10441 aaccggtagc gaccatcaag aatccgccgc aagctttaaa atctaagatc atgtctggtg
    10501 tagtcaatgg ataaataaat tttgccaata agaacaaaat ataaaaaata atgaattggg
    10561 gaattgctat cacagaaacg acatacccta aattacacgc aaaaatagct gctgtgaaaa
    10621 aatcaaggac agatttagaa atcaaaatcg tactgtctcc tgtcatccca gaatctaaac
    10681 ttccatagat ccccgtacca cttgcacaaa atagaacaat gaccgtcact aatgtgctga
    10741 taaattcatc gtgggtcata ccaagctttt cactagggaa gattttagaa ataggtactt
    10801 gcatcagcgc agccccctta tttatcccat tacctaaatg aacaatcaaa ccaatcgctg
    10861 ttcctatcac taaagcaaaa ataacagccg gcatattttt catcggcgca atggaataga
    10921 ttcccatccc catcgaacac acaccaaaaa ccatcgtcag ctgtgcttta aaatgttcac
    10981 ttaatttatt tcctactaat ccgccaatta gtccgccaaa caaaacggaa cctacattga
    11041 tcaaaactcc agttggcatc aaaaaactcc tctctttttc tacgaagcga aaaaaatcag
    11101 aaaaatttct gtaattattg ctttcgctgc aagaaagatt gtaacagaca gagaagatta
    11161 caccaattca ttttttgtgt taaagtattc tttattggaa taagtaggag gaatatgatg
    11221 gatatccata aattcgaagt ttttcttgat ttagccgaaa caatgaattt caccaaaact
    11281 gctgatcgac aattcaccac ccaaggaaat atttctaaac agattctttc gttagaaaca
    11341 gaattagacg tcaaattatt cgaacgcgcc aatagaaaaa tcgaattgac cgaagctgga
    11401 agcttattgc tcccatatgt aaaaaatgtc gtagaacaat accatgctat gcaagaagtg
    11461 cttgaaacct atacgaaaga caacaatctt tcattaaata tcttaacgat accgacaatg
    11521 atcaattata aaggtttttc caaaatcacc gagtttttga aacgccatcc tgaatttaca
    11581 gtccagttga aagaagtgga aagtgtcgaa ttaacttaca atgacgccaa tgaacatagt
    11641 gacacgatcc actttgctag aagtttccag gccccaccaa gttcgattga gtggttgcct
    11701 actgaaacag acgattttgt agcagttcta cctaaaaatc atcctttagc tagtaaaaag
    11761 aaattggatc taagcgaact gaaaaaggag aactttttgc tccttggccc cactacgaac
    11821 ttgtatcaac ctgtactaga tctgtgtcgc gaagctggat ttgaaccaaa aatctcttat
    11881 aaaggagcaa gaatcgactt gatcatcaat atgattgcaa atgacatggg gattgctatt
    11941 gttatggaaa aaacagtcaa gaatctatta aaagaaaata ctgtgattct ccctatttct
    12001 ccaacaacag agagttatct tgcatttact cgtaaagtcg gtgagcattc tttggcttca
    12061 gatacatttt gggagtattt aaatcaagaa gaaaactaag ccctacattt tctgtccatc
    12121 tatccaccca aaaaaagaga gcgacgcatg actttcgctc tctttcttgc atcatgtcct
    12181 tgtttcaatg acacctctaa catttctcgt agttcaataa gaaagcagcg atttttatgc
    12241 caaaagaacc gtaagaacat ttccctaatc acaaaaaaac gactaaaaac ccctaaaaac
    12301 cttcaataga atttttaggg gttttattct ttttcctatt gtttacttaa ccgtatcatt
    12361 gctgtcgttc ctactccttc gatactgcta taatcaatac gtccatcatg cagttctaga
    12421 atttttttaa ccagtgaaag tcctagccca ttcccagaaa tttgacgact tgtatctcct
    12481 tgataaaatt tttcaaacat tcgatctctg gtctcttcat tcattcctac acctgaatcg
    12541 gtcacttcca gtacaatctc tgtggctgtc tcaaatagtt tgatatgaat ctgtccattg
    12601 atttgattgt actttatggc gttatccatg atattcagcc acacttggta caaaaactct
    12661 tcattgcctg tgtaatttac tgcagcaagt tcgatatcca attctagctg ttctttctcc
    12721 catttaggct gaaaaaaaac gattacttct ctgatttgtt catctaatcg atactctttc
    12781 ttttccagtt ggatacgctg gttttccagt ttgttcaatt ttaagatgtt ctctgttaat
    12841 tgtgaaagct gcgtgatact ttcgataatc cgatataaaa atatttggcg ttcttcgtca
    12901 gagagatttg gggcttgtaa caattgaaca tatccttgga ttgttgctag tggtgttttg
    12961 aattcatggg atacactgga cacaaagtca ttcctcagtg tctcgatact attgagttct
    13021 tggaccataa cgttgaaatc tttatacagt tgctgtactt ctgctacttt ctgctgttca
    13081 tccattcgaa tcgaaaaatc tcccgttgct accagactca tatttgtccc tagcgcactg
    13141 attggatgca ggatacgccg cccaacaaaa atcgatattc ctgtacccac gaccatactg
    13201 aaccccgcaa agacgaataa cgggtataac gttggttttc cagccgattc agcaggcgag
    13261 atagaatgtc gagacaataa gaaaataaaa ccgagaaaac acagcagtgt cactaagatc
    13321 gaaatgaaga caatactagc aaaatagatc cataattgtg aaaatagctt ttttttcatc
    13381 tttaaataac cgccttatag cctagtcccc gtactgtgat gatttgaaaa tcttccgtct
    13441 tttcaaaacg ggtccttaaa cgtttgatat gtacatcgat cgttcgttct tctgtattcg
    13501 tatctaatcc ccaaatatca tccatcaact gttggcgagt gaagatcttg ttgggataag
    13561 acagtaattt atacagaagc agaaattctt tttgaggcag aatcaacgaa tgctgatctt
    13621 gtaacactgt ataagcatct agctctaatt ttgtttgtcc gattatcaag ctactgctgt
    13681 aattgatctt tgaacgtcga agcaatgctt ccacacgtag gatcatctca ttgacatcga
    13741 tgggttttgt catataatca tctgctccta atttgaaccc gcgtttctta tcctcaaaat
    13801 cggcctttgc cgtaatcatt agaacaggtg tgtcaaatct ggtgctacgt aatgagtcta
    13861 gcaagtcata gccatccatt ttcggcatca tgatatctgt aatgattaat tctatacgtg
    13921 tcgcttctag ttttttcagc gcatcctctc cattcaatgc ggtgatgact gaaaagcccg
    13981 ctcgttccaa cacagaacgg taaagcaact gtaattgcgg atcatcttct actactaaaa
    14041 tacgattcat tgatctccct ctgttttttg aaaaatttct agattttttc ttgtagttca
    14101 tcttcagtta acatttattt cgtatactac gatcattgtt ctgatatact tattagtata
    14161 ccgaatattt caatacggga gaagggtgat tatgcttcaa ttaaaagata taaaaaaata
    14221 ttataaagtc ggcgaaacca ccaccaaagc attggatggt gtgtctgtcg ctttcagaca
    14281 aaaagaattc gtagcgattt taggtcctag tggttctggt aaaacgacta tgctgaatgt
    14341 gattggtgga ctagataatt atgattccgg tgatatggta atcaacggaa agtctactaa
    14401 agactttaag gacagtgact gggatgccta ccgaaacaac tctatcggtt tcatttttca
    14461 aagttacaac ttgattggtc atctaggcat tattgaaaac gttgaacttg ggatgacatt
    14521 aagtggtgtt tctaaagacg aaaaacgaaa acgggcagaa gatgcattgc accgagtcgg
    14581 cttgacaaat catatgcata aaaagccaaa tcagttatct ggtggacaaa tgcagcgagt
    14641 ggccatcgct cgagcactag ccaatgaccc agatatcctt ttgtgcgatg aaccaaccgg
    14701 agcgttagat acagaaacaa gtatccagat catggaattg atccaagagt tatcgaaaga
    14761 aaaattagtg atcatggtca cacataatcc tgaactggcc catcaatacg cagatcgaat
    14821 cattgaattt tctgatggta agatcttaac agattcccat cctcacatcg aacgaccaaa
    14881 agatgatcaa tttaacttac gcagaacaaa gatgagtttt tggacagcgt taaagctttc
    14941 cttcaacaat atccgaacaa aaaaaggacg tactttcttg acctcctttg cttccagtat
    15001 tgggatcatc ggaatcgcga tcgttctgtc cctttcttct ggattccaaa aacaaatcga
    15061 taacacgcaa gctgaaacga tggcgaaatt tccgattact atttctaagg taacgactaa
    15121 ccagactcgt gacgatgccg gattgggtgc aagcaaagcg gattatccag acagcaaaac
    15181 gattactgca aaagtcagcg aagaagataa agcacagcac acgaataaga ttgatcaaac
    15241 atacgtcgat tacgtaacag acatcgatcc taacttaagt aataatatcg gatttacccg
    15301 tactacagga atcaatcttt tacgtgatgt aaatggcaaa gtacagcctg tcagcttctc
    15361 gaatcaaaat cctgatacgg aatctctttc tttatctagt acgatgtcag caatgacagg
    15421 tgtgggagtg tcaagtttcc ctacacagct tgacactagc aaagaaaact ttttaaaaga
    15481 caattattct ttacttgccg gaagttatcc agcctcagca acagatgtcg tgctgatcgt
    15541 agacggcaat aataatacta atatcaacgc attgaagaat cttggatttg atgtgaaaga
    15601 agacgaaaaa cttgattttg atgatatcgt tggtacaaca ttcaaattag taaacaacaa
    15661 tacttactat acaaaacttc caactgggaa ttttatccct aatacagatt atgatgcgat
    15721 gtatcagaat gcttctgatg aattgaagat ctcggggatc ctacgcgtga aatcttcttc
    15781 tacaatgaat ctcttatctc caggaatcgc ttatagcgat caattaacga ctcaaatcgt
    15841 caacgaaaac aaagaatcag aaatcgtcaa agctcaaaga gacagtggtg taaatgtctt
    15901 aactactgaa aaagttgacg aaaatgccaa acaaacattg cttagttatt taggtggaga
    15961 cagcttgcct tcaagtatca tgatttatcc gaacaatttt gaagacaaag aaaagatctt
    16021 ggattatcta gatgattaca ataaaggcaa atcagatgaa gataaaatca tttatactga
    16081 tttagctgga acgatgactg aactgactgg cggattgatg gatgctatca cttatgtcct
    16141 tattgctttt gcagggatct ctttagtaac aagtatgatc atgatcagta tcattactta
    16201 tacatctgtg attgaacgga caaaagaaat cggtgtacta aaagctttag gtgcacgtaa
    16261 aaaagacatc actcgcgtat ttgacgcaga aacgtgtatt ttaggtattt cttctgggat
    16321 attaggtgtg tttattgcat ggctagctac gttccctatc aatagcatct tatacaacat
    16381 gacggatcta aaaaatgttg ctcaactaaa tcctgtccat gcgattattc tagtgatcgt
    16441 ctctactata ttgacgatgc ttggcggtca tcttcctgca cgaatggcag cgaaaaaaga
    16501 cgccgctatc gctttgcgtg cagaataagg ttgtgacaac cacatttaga tttgagtcat
    16561 aagagcgctg aaatagtaga aaacagcaag aaacgaggaa gtaatatgtt ccttgtttcg
    16621 cgctgttttt tttaattatt ttttataatt ctggtatact tttctttcct ttgtaccaca
    16681 actcttctgg cgtatacctg tcttttccat ttatgaatac tgttccatct gcttcaatcg
    16741 ttacttcagt cccaatccac atattacgag aaagtgcgag aagatttttt ctttttcctc
    16801 ttcctttttt tccaaattgc aaggcatcgg ttatctcttc tgtccaatga gatttcacgt
    16861 attttacttc tccgttttca aatgtaactt tcaaaatatt cgctatattt tccactttta
    16921 ctgctttagg aaatgactcc tccatttttt tcactttcct tcctccaaca ctaagtatgc
    16981 gctttctttt agtgataagt atatcatagt ttattctatt atcaaaaaaa cgcatccatt
    17041 ccattgtaga atactttacc gaacaagttg aattgttttc ctctttttca ccttttacta
    17101 tagataggta aagacaacta aggaggaatc tctcatgaca gatcaaccgt atgatttaag
    17161 aaaagtcatc gaagaactaa aaacacttcc aggtcaatat cacgaaacag atattgaaat
    17221 cgatcctgaa gcagaattat ctggtgttta ccgttatatc ggtgctggcg gtacagtaaa
    17281 acgtccgact caagaaggac caactatgac tttcaacaat atcaaaggtt tccccggcac
    17341 acgtgtgaac atcggaacaa tggctagtcg taaacgtgta ggtgaaattt tgcaccatga
    17401 ttatcgaaca cttggtcgtt tcttgaaaga cgctgtagaa aatccaatca aacctgtgaa
    17461 agtagacaaa gcagacgctc cagcacaaga agtcgttcat ctagctaccg atgcagactt
    17521 cgatattcgc aacattttgc ctgcacctac aaatactcct gaagatgctg gtccatacat
    17581 cacaatgggt gtagtcttag gatcagatcc agaaaaaaca atgagcgatg taacgatcca
    17641 tcgtatggtt ctagaagata aagatacgat cggtatgtac atcatgcctg gcggacgcca
    17701 cattggtcac ttccaaaaac aattcgaagc cttgaataaa ccaatgccta tcactatcaa
    17761 tattggttta gatccagcaa ttgccattgg cacgactttc gaaccaccta ccactccact
    17821 tggctacaat gaactatggt gtgcaggagc tttgcgtaac gaacctattc aaatcgttga
    17881 tggtatagca gtcgatgaag tgggtattgc tcgtgctgaa ttcatcattg aagcagaaat
    17941 catgccaaat gaaacaatgc aagaagatat caacacgaac tcaggaaaag caatgccaga
    18001 atttcctggc tatactgggg atgctaatcc agctgtacaa gtagtgaaag tcaaagcagt
    18061 tactcaccga aaagacaatc caatcatgca aacaacgatt ggtcccagtg aagaacacgt
    18121 atctatggcc ggtattccta cagaagcaag tattcttgcc ttggtcgata aagctattcc
    18181 aggaaaagta gtaaatgtct acaatcctcc tgcaggtggt ggtaaattga tgaccatcat
    18241 gcaaatccgt aaagaaaacg aagcggatga aggtattcag cgtcaagcag ctattcttgc
    18301 tttatcagcc ttcaaagaat tgaagacagt cattttagtc gatgaagatg ttgatatttt
    18361 cgacatgaac gatgtagttt ggactttcaa cactcgtttc caaggagata aagatatcat
    18421 cgtgatccca ggaatgagga accatccatt ggacccatca gaattaccag aatataatcc
    18481 tggctctatt cgtttccgtg ggataagtgc aaaaactatc tttgacggta cagttccttt
    18541 cgatatgaaa gaacattttg tacgtgcaaa attccaagaa gtagaagatt gggaaaaata
    18601 tttgaaataa tgaatgatct gcaactgttt tgacatcata aaaaggagtg cggaaaatgg
    18661 aacaattggc acaacggaag aaaaaaatcg tgattggtgt aagtggcgct tcaggcacca
    18721 tttatgccat caacctagtg aagaaactaa aagaatatcc gatgatagaa acacacggaa
    18781 tcatcagtcc atgggcaaaa cagaacttaa agctggaatc cgacttatca ctaactgaat
    18841 tcgaaagcct gtttgattac cattattcaa acaaagattt aggtgctgct gtcgccagcg
    18901 gatcattttt gaccgatgga atgatcatcg taccggcaag tatgaaaaca gtcgcaggta
    18961 tctctgttgg tttaggcgac aacctgattg cacgtgctgc tgatgtaaca atgaaggagc
    19021 aacgaaagtt agtcattgtt cctcgtgaaa cgccattaaa tgccattcac ttggaaaact
    19081 tgactaaatt agcacgacta ggtgtgcaaa tcataccacc aattcctgct ttttataatc
    19141 agcctcaaac gatccaagat cttgttaatc accatacaat gaaacttttg gatcaatttg
    19201 aaattgtaaa tgagcattca aaacgttggc agggtgatta atatggaaaa agaaacgata
    19261 gttgaagtaa ccaaactagg tttcacgatc caagctgctg ttaaagtaat cggtaaagac
    19321 ttattgatca ctttaacagg cggagataca ccacatatcg gaacagtcac tacattttct
    19381 cgtgatacag agatgcaaac tgtacgtttc cctagccacg atggtcggtt ccataaagac
    19441 gatgtcttag ccgaaaaaat agctgtgatc atcaaaactg ccgttccggg aagttgtacg
    19501 atcacttccg gtatccatgt tgaccatatc tccaatgcac aaatcaacgc ctcttttccg
    19561 atgtcagaag agttaggtta caagatttta gattggttgg aaaaaataga tttccatatc
    19621 aaagaaccag tttattataa agatggtgaa aaaccattat gaaagaagag gttgtgaaat
    19681 cagcgttatg acctgagtat gagagaaaaa aatggaaaaa tagcttctca tattttgccg
    19741 tttttgagct aataccgagg gacagctgat tgaacgccgt ttatcaagag gttgtgataa
    19801 agccgcttcg tttcgagtag taagaaaaat tttgtaaaca tgggtttcca catttttgca
    19861 taaatttgac ttactaccga gaaactggct tttgaacacc gtttatcaag aggttgtgac
    19921 acgtcttttg tcacaacctc ttttattttt gcttcactgt aactgctaca atatcaccga
    19981 tttcttttcc gatgttttta cgaatatctt ttcgtagtcc aaggatgtag cagactgaac
    20041 catctggatt tttcacgccc atattcacga tactgccttc atatggcaca ttgtcaaaag
    20101 ttgcttggac tttaactctt ccagcaccga attcttttct tatatcatac ggaaaaataa
    20161 tatatgcccc gcccttgccg acttcggaag catagatttt tgattcaaaa gaataaattt
    20221 ccctcatttt atttctccta ttttttacat ctagtatagc atagcaaaaa aaagaaggaa
    20281 ctgtgacaaa agtcttgtcg cagttccttt tttcgaataa acggtgttct cctgccaact
    20341 cctcggtcac aagtcacaat agtcaacaaa catgagaagc tgttttttga aattgctcct
    20401 tgtgactcag agttaaacgg caggctcaca acctcttcct taaacggtgt tcaaaatatt
    20461 attcgccttt tgcttctttc actaattttg cgaatgcttg ttgttggaat tcagcagcta
    20521 aggctttcgt acgttgttct tgctctgctt tgctcatttt tggattttca gtcattgctt
    20581 ttaccatacc gtcttgaaca taagcttttg cttcactttc catcgtactt tgctgtttct
    20641 tcactaattc agctaacttg gctgcttggt ccgtacttaa ttctacccat gtctctggga
    20701 ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt atcagcaata ttgaaccaga
    20761 atttatacat gtcgtttttg tattcccaat aagtagtttt cgtcactttt tcagcatcgc
    20821 cgtctgtctt ttctacttta tttgtttcgt tatccgtacc agttgctttt ggtttttctt
    20881 gtttttcatt tgttcggtaa agatacactt tttcttttcc attccctaaa gctttataca
    20941 aaagcatatc tgctccttgg gaatcagcac tggaaacaat tttttctgtt tttgtttccg
    21001 ttactttttc cattccaaag tgattggaca aattagcgac gatcaaactt agtgaagcga
    21061 cgaagattag tccgaaaatc aaagataaaa aggtttgcca tgttttttta gcgaacacgt
    21121 tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc aataatcatg cattatcccc
    21181 cgctttcatt agaacaggtt cttttttctt caataaaaag gcaataataa agccaagaat
    21241 cccaaagatg atcgctacga taaacgcggc atggtaacct gacagtgtgg cattgattgc
    21301 ctgatcttta taagctaatg gcgtattatc caatacagat ttgcttggga tatttgcttt
    21361 tgtcacattc gtcaacacac tgatcaaaat agctgtacca atcgagcttg cgatttgacg
    21421 gaatgtatta tttaccgctg tcccatgact gatcaagtta tttggcaatg cattcatacc
    21481 agaagtcgtt accggcatca tgaccatgga gatcccaaac atacggatcg cataaaggat
    21541 cacgatatat aaaactggag tcgtttttgt caagaaagca aatggtgcag ttgccgctgt
    21601 caaaatgaac atccctgtga tggctaaacg tttcgcgccg tatttatcga aaatcgcacc
    21661 cgtcacaggc atcatagccc ccatgatcaa tgcacccggc aacaacatca agccagaatg
    21721 gaatgctgat tctccacgaa tattttgaat atacaatggt acgaccattt ctgcccctac
    21781 catcgccata tttgttacac cagctaaaac agctgcaatc gtgaatgtag atgatttgaa
    21841 tacacgaagt tccaagaatg gatgttccaa atgtaattga cgccatgaga acaaggcgat
    21901 gaaaatcaca ccaacaacta agaaaccaat tactttcaag cttccccagc cgtcatctcc
    21961 tacactagaa aatccgtaaa gtaaactacc gaatccaatc gttgataaaa tagcggataa
    22021 aatatcaatg ctaggatttg acaatggaat aacacttttc attaggaaga atgctaaaac
    22081 caagacaaga acaacgattg gcagtaccat accaaacaag tcacgccaat ggtagttatc
    22141 aatgatataa ccagataatg tagggcctaa agcaggagca agtccaataa cgatccctga
    22201 catccccatt gctgatcctc ttttttctgg cgggaaaata gatagcatga tattttgcaa
    22261 taatggcatc gatactccca caccgcaagc ttggatcaaa cgaccgatta gtaatgtact
    22321 aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa aagatcgtca tggcagaaag
    22381 atataaattt cttgaactga atttattgat cagccatgct gttattggga tcatgatccc
    22441 gttgactagc aagaaaccag tcgttaacca ttgcacatca gacgctgaaa tatcgaaagc
    22501 tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga ttcaaaacgg tacaaaatgt
    22561 accaattaat aagacaacaa ctaaaagtgt ccgattatat ggttttccgt aaatatctac
    22621 gggttggttg ttactcatat ttctccttct ttctgaattt tttctgaaac tactagaata
    22681 ctttagtcct aaattcgcta tttgtcaacc tacttgacac ataagttaat acggatataa
    22741 ttaagggaag caagaggaca aaaggagtga caaaaatggt tggggcttcc cttaaacgtt
    22801 tgttggaaaa tgatcaattg gttgttggca tcagtgaatt aagtgatatc gtaggtgtgt
    22861 ctccacgtca attaaggtat tgggaacaaa agggattcat tcgttcgatc gaagctgatg
    22921 cgaattgtcc tagaaaatat cgtttaccta cagtcataaa agtagaactg atcaaaaaat
    22981 atttagatga cggatttact ttgtcaaaag ctgcagaaaa ggcagaaatc cgccaaaaga
    23041 aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa agatatcgaa ttgatcgacg
    23101 aacgctatac gattttacaa atcggtaact tcaaagaaga gaatgctaag atgtacatca
    23161 ttcatgacaa cgagacagac gaattaactt acaagctcct tcctgtcgat gaaaaagctg
    23221 actacgaaca gttgttaaaa gaactctaaa cgccactaat ctcctaagta ccaagctttt
    23281 taataagtag atgctacata aatacttgaa aaaaggcctg tttgaacata agaatatccg
    23341 aacggtgatc tgcgtttctt tcatatagaa aggaattctc catcactgtt cggatatttt
    23401 tttatttttt tcggttactt tcttctaata catctgttag ccaatttgca tattctgttt
    23461 cccgattgat cgcatgcgct aaaatcaggt agtgtccgta attttcatgt ttttcttttt
    23521 cagtaggaaa gatcgttttt tttcgctcta atagatgaag tcgtttttct tcatgaaggt
    23581 cacgctgctg ctgaataatt tctactaatg ccggatcatt cacgtctttg acgaaataca
    23641 atttcagaac aaattcgtct cgattaaccg gtagttcatt ggtgggtgta tgaatccact
    23701 ctgccaattg ttccttccct tcttctgtta attcataaac tttcttttcc aatttttcac
    23761 ctgtgatctc tacttgatgc tgaatgattc cttgctcttc catttttgca agttcaggat
    23821 aaatctgact atgttttgct tgccaaaatt ctcctatttc atttttgaac gattgattca
    23881 attcatatcc tgtcatcttc ttcttactta ataaccctaa caaaatatat cgaagtgtat
    23941 tttttcttgc catcagtttt ccctccttga caaatcttta aatccattct atcataaatg
    24001 agtaatcgaa aacatgtagt ttattacatg ttttaattta taaggagatg cttaaacatg
    24061 aaagaattta aagaattgga agattttatt ggaactcact ttatctatac gtatgacaat
    24121 ggttgggaat atgaatggta tgccaaaaat gaccatactg ttgattaccg tatccatggc
    24181 ggaatggtca aaggccgttg ggtaaaagac caagaagctg atatcgttaa attgacggac
    24241 ggcgtattca aaatcacttg gacagagcca acagggacag acgtggcatt ggactttatg
    24301 ccaaatgaaa agaaacttca cggaatgatc tttttcccta aatgggttga agaacatcca
    24361 gaaatcactg ttactttcca aaacgaacat atcgcagaaa tggaagcagc tcgtgaaaaa
    24421 tatgaaactt atccaaaatt acttgttcct gaatttgcta cgatcactta tatgggtgat
    24481 gcaggtatag acaatgaaga tgttattagc gaagaaccat acgatggaat gccagatgat
    24541 atccgtagcg gaaaatactt cgatgaaaat tataaacgag taaaataagg ttgtgaaaga
    24601 gaggttgtga aatcagcgtt ctgacctgag tattagagaa agaaacggaa aaatagttcc
    24661 tcatgttttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca
    24721 agaggttgtg aaatcagcgt tatgctttga gtaataagaa cgctagttag aaaatagctt
    24781 ttcatatttt ctaggctagt gggcttatta ccgagaagca gccgtttgaa caccgtttat
    24841 caagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaatg gaaaaatagt
    24901 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
    24961 tcaaagattg taacaactgc atattcacac agttaaatta gtgacttcaa aatgaaataa
    25021 tgctcccagc aaaaaattag gctgggagca ttatttcgtt attctgttca tcaaaaatca
    25081 gcgttttgat tccatttcat cacaagttcc tcctcattta ctgcttcatc tttttgctgt
    25141 gtttgaattg taaaaccttg tgaaagatag aaatgaatag ctgattgatt tttttgatag
    25201 actgtcaaag tcagtgtttt attggtttct tttatttttt tcattagttg cgtaccgatt
    25261 ctttgttgcc ggtattccct tttcacaaag attcctgcga taaactgatc aatcacccca
    25321 gcaaaaccaa taatttcttg atctatttct gctaagtaaa tcactgcatc tggtaaagct
    25381 ttcctaacag attctacttg attctcccaa taagttgatg gaatgaattc atgagaatcc
    25441 aagttagcat gcagccaaat cgatagaaca gattccagtt ctttggcaga caattttgtt
    25501 gttgtgcgga tcattttttc tctccttact tattcattga ttttaaaata aaaaccattt
    25561 ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt atatcttctc ttgttttact
    25621 tttctaatac caaaagaaac agcaacttta ggatcatcag ctgaaacgat tcgataagtt
    25681 ccaccaggca attgatccag cttgactttc ccatgatggt cgctttgtac tgaaaacggc
    25741 acaccatttc tagtaagtgt ttttttctta tgtttcaaaa tgaatgaact accatttacc
    25801 ggtttccctg acgaatcact aataataaat gaaaaatcaa gcactttttt acttaatcta
    25861 tactgataga ttagtcgtcc gataaacact aacagtatga ctacagctat tatcgttcct
    25921 gcaagaataa accattgccg catggttctt tcctgatttc cttttccttc ttcttttttt
    25981 accgattcgt tataaggaat ccgatgtcct gtcactagca aacgatgcgt gttgatcata
    26041 tacggcgtac atgtaagcaa ggtaactaag tcttctcctt ctgaaatcgt tagaacagac
    26101 gtgtcatctg gcagtaccac ttgtatattt ttaacttgat aagccaattt ctcccccaaa
    26161 acagtcaaaa taaacgtgtc tccgttttct aaacgatcta attcggtaaa taaccgtcga
    26221 ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg aatttccacc aagcgggaaa
    26281 gaggttcctt gcaagacgcc tgctccattt tcaaggatcc taccattgag ccgatcataa
    26341 agtgaaatat ttaattggat cttaggtata ttgatacttc cgatcagcca ttcctctaat
    26401 tggctggatg ttgtactttc atttctgttc ttttcattga aaggatcagt accaggattg
    26461 aatccttggt cagttaatcg ttggtttttc tcctccattt tctttcgctg cacttcaatt
    26521 tcagctgcgg tccgtttttg tacttgttcg atacgttttt gatcaataaa actatttaaa
    26581 gaatccacat aaaatggata taaagcgatc agtaacccta cgacgataca tattaataaa
    26641 aacaaacgaa acaaaagtcg acgagactgt ttttgcttac tctgcttcac atgtgtcctc
    26701 ccttgcaagt ttttttgttc tttcttttat tatatgggtt caggaaaaaa atagaaaatg
    26761 aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt aataagtaca gcccaaaaaa
    26821 atagctactc atattttttg ttgggttggg cttattaccg cagatgcaag cttttgaaca
    26881 ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg agtaataaga gcgctagtca
    26941 gaaaatagct tctcatattt tctaggctag tgagcttatt accgagaagc agctgattga
    27001 acaccgttta ccgagaggtc atgaaaaagc tactttgttt cgagtattaa gaaaaattct
    27061 gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc ctctctcctt agaactttca
    27121 cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa ttttctaatt tcttgcctac
    27181 ctctcgcata ggatatatga tgaatttatc taaagtttgc aaaggagttc gacaattgaa
    27241 aaaatatggc ccatttttag caattttttt cattgctttg gcatttctca tgcctcattt
    27301 attcacacat aaactaattt tgggtgctga tgccccattc cattacaatc ggttctatga
    27361 tgccgcgcaa caaattaaat atggggattt ccattatttt ccttcccttt atggattcca
    27421 gcaaagtggc aggatcgtga atgccgttta cggcccattt ttcgcttatt tacaaggatt
    27481 gatcgtgttg cttgcaggta gctggtttca ttatcagatt ctttcaaatt tcattcttta
    27541 tcttatttca ggattttcta tgctgatttt actgagatat gtcaatgttc gaaattgggt
    27601 aagtgtacca gtagccgttt tattcatgag tacgtattct attcaatact ggtctatcaa
    27661 ccaaggattc actagctggg ggacttgttt ttatccttta tgtatgatcc ctattgctga
    27721 tttcgtcctt cataaaaagt tccctgttat caaagtcgct gtctcagtgg gacttatgac
    27781 acagatccat ttactgtctt cgatcatgtt agctttgatg tatatccctt tttatcttta
    27841 ttactttttt aaccagactg aaaaaggaaa agcactgctt caactgatta agtcggtctt
    27901 gctttatttt gttctgacag caaatatttg ggcaggatta gctattgttt acagtgggaa
    27961 taagattctt cctccctttg ttaacagaga aatgagtcaa aaggcgatag atctggatgg
    28021 cagttattgg ctccattacc ctaaggcctt tcctatattg atattatcag gttttttgct
    28081 ttttattttc tttcaacaaa agaacacctt attccaaaaa atcgtgttgt acacaagtct
    28141 tgttttcttt tttctttcta ctagcttgat tccatggacg acattgatcg aaaaaaatgt
    28201 gccttttatt agtttgatcc agtttccatt tcgatttttc gtgccattta cgctattgct
    28261 tctactttac cttgcccttt cactgaacca gtggtctgaa caaaagtggt tccgtctttt
    28321 aagtatattt ggcattctta ttttttcagc acagacgatc cagaacttat accagcatct
    28381 ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact tatctattcg atacgccgga
    28441 agaagcaagg aaaacattct tcagcaaaga tatgtctacc agcttgttcg ttttccaaaa
    28501 aaccacacct gactatctgc caatctataa ggagacaaaa gacaataaat acgaccggta
    28561 taacgaagaa atactagaaa acgaaacaac ttttataaaa acacatccag gcggtctttt
    28621 gacaatcaag tggaaaaaca ctggggacaa aactgttcat atccctgtta tcgtctacga
    28681 tcggactgtg ctacagcaaa atggaaaaac attgactgat tatgaagtaa cagatatcgg
    28741 gaccccaatc gtgaagcagc aaaaaggaat caacgagctc acgcttcatt atcaaacgcc
    28801 aatctatttc tatttcattc tttctcttac tttgattggc tggtttacct tgctatgttt
    28861 atttatttat catagataca agcttttacg tgcttaggca aaagggataa atacatccga
    28921 tcaaaaaact ctccaaacgg tataatcgtt tggagagttt tattttaatc agttttttta
    28981 tgttatatgt tatatttttt attcgttcac atctaattcg atttccaccc cgtagtgatc
    29041 actgatgatt ggactatcga tcccatcaaa aatccgttga tattttttaa cctgcattcc
    29101 ttcagggacg aaaataaaat ctatacgcaa tttttctgta ttttgttccc aaccatctat
    29161 tttcttttcc acagtagctg cttttccttt ttcattcgcg acaacatacg aatcttgaag
    29221 aagaaggcaa ctttctctta cagtataata accttgagag tctactggat tattgaaatc
    29281 acctaggaaa aaaagtggta cgcgagtttc caaaaagtag ttttccaagt ttttccactc
    29341 aaaataaaag ccactttttt tatctgtcca ccaagaaaag tggcagctcg agacaaaaat
    29401 gtcctgatca tctatctttg ttttcccaat caagatcttg cgtgtctgat aatcttcttt
    29461 cttttgaaat tgggagacgg taaggacctg actttccaat gtttttttcg taagcagtgc
    29521 atttccttct tcatatttat cataaccgat gtggctcatt tcccaactcc aaaaatactc
    29581 ttttccgtgt tcctttaaaa gctgaactaa acggtaggca aaattatctt tacggatagg
    29641 tacttgattc tttaccggac agaacttcat tagcttcgca ggttctactt cttcagattc
    29701 cagtaattgg ttgatttctt gtaaagcaat cacttcataa tcttctaaca ggatcttatc
    29761 tgctaattct tgcaattttt tctctggttc tggctcaagc cagctatgcg tgttgagtgt
    29821 catgaatttc attggttttc tccttgtctt catttcgaaa caaaagggtt gtgacaaaat
    29881 ttttgtcaca acctctccag caaatagtgt tctcctgcta actcctcagt aataagctca
    29941 atggaccagg agctattttt tgtttggctg tttttatcac ttggtgcagg gcagcttttt
    30001 cacgacctct ccactaaaca gtattcaaac tgctgcttct cggtaataag cccaaccaaa
    30061 cgaaaaatag gaggagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt
    30121 tttcacaacc tctccttaca ttttaatgga gccgatcact ttaccagctg tcactgtacc
    30181 taattgcttg atcactactt cttctacttt atcgtttgta aatacgatca tgatcgttgg
    30241 atctttttct tcgttttgga tcacatcaag atccatctca gctaatttcg ttcctgttcc
    30301 tacttcttgg ccttctttga cagcaatctc aaaagctggc tgattcattt ggactgtatc
    30361 gattcccata tggatcaaaa cttcgatacc atctttcgtt tccagcccaa ttgcatgttt
    30421 gctagggaaa atacttgtca cttttccagc gatcggcgca acaacttctc ttgatgttgg
    30481 aataacagca aagccgtcac ccatcatttt ttgtgagaat accggatctt ttacttcaga
    30541 taaaggaatc acttctccag aagctgcgga aactagttct tgttctatac ctaagtattg
    30601 cttactacct gcttgtccat ttcctgtaga aaccatttct ggctctggaa tctctgctcc
    30661 tgattgcagc agatcttcga tatctgattt caagacatct gcttttggtc cataaactgc
    30721 ttggactcca ttgtctttga cgatcaatcc catcgcccct gctcgtttcc aggcttcttc
    30781 agtccctact ttttctcgat catttacact tacgcgtaac cgagtcatac aagcatcgac
    30841 atctttgata ttttcttttc ctcccagcaa atgaatgatc tggaccactt gctgatctgc
    30901 tgttcctgtt ccgctgttag atacaacggt ttcttcactg tcattgtcgt aatttccgtt
    30961 acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa tttgctaaga aatacgtaac
    31021 aacaccaaaa gcgatcacac acaacacgaa gttgataaga tctccaccta gacccgcttt
    31081 gatagctaac ggtgtccgag tcagcaattc gatattccca aatgaatgga cccgcaatgg
    31141 caagatatct gccatcgcaa atgcagcccc ttggatcact gaataaatga catataatgg
    31201 cacagcagca aacataaaca tgaactccaa tggttctgtt acacctgtta gaaagacagc
    31261 caacgctgct gaaaaataca ttgatttata tcttgctttc ttatcaggat cgacattacg
    31321 atacatcgca aaagccattc ccatcaagat acctgaagaa ccaatcattt gtcctacttt
    31381 gaaacgagcc ggcgtccagt tatgcaacac aaactcatat tgcgacatat cccctgcacc
    31441 tttaagattc actaaatcag tggcccaagc caaccataac ggatcttgcc caaatacttg
    31501 tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg ccaagctgtg tatagttgat
    31561 aggaatcgtc agcatatgat gcaatccaaa tggcaatagt aaacgttcta atgttccaaa
    31621 taaaaacggt gctagaatcg gtgcgctttc ttgtgattct gcaatccata aaccaaaatt
    31681 attgattcca gcctgtacgt ttggccacac cacagcaaga atcaatgcca cgattgttga
    31741 ccataagata acaacaaacg gaacaaagcg ctttccattg aaaaatgaca aagcatccgg
    31801 caattttcta taattatagt acttattata agccattgcc ccgacaaatc cagcgatgat
    31861 ccctacgaat actcccatat tcaaagctgg tgcttccaat acacttgtaa aaaatccttt
    31921 aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca tcttcattcg caagcatgtc
    31981 acttgtgaca ccaaagatag ccccagtgat ccgattgatt aaaataaatg agatcccagc
    32041 tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct cctattgcta aagcaaataa
    32101 aatatgaaga ttaccaataa ttgcccaacc aatattttca attactccgc ccgttgtgac
    32161 aagcggcgtc caatcaggat tgatcaaggg aattgatttc cctatactga tcatcaaccc
    32221 agctgccggc ataactgcta ccacaaccat taaagccttt ccaaattttt gccaaaactc
    32281 aaaactaaat atctttttca ttccgtttag ctcctttacg caatcggttt cgttaaacgt
    32341 attatagcgt gcgttttctt ttttgtaaat gctttatttt ttatttttct gtctatacca
    32401 ttctataaaa cctataatac gtgtgttccc ttctataatt ttctcctaaa aatttatttt
    32461 tttaagcttt tttctatgaa atattttctt ttgtctattg acttgtgcaa tcggttgcgt
    32521 ttattatgaa cgtataaaac agctcattag gaggagaaaa atgaaacaga tcaaacgact
    32581 atttgctatt gatccatgga aaatcagcac gacacaatta gataaaaaaa atcttcgact
    32641 gcaagaatct ttaacaagta ttggaaacgg ttatatgggg atgcgcggga actttgaaga
    32701 aacttactca ggggaccacc atcaaggtac ttatttggcg ggtgtctggt atcccgataa
    32761 gacacgtgtc ggctggtgga aaaatggtta tccagaatac tttggaaaag tgatcaacgc
    32821 cttgaacttt atcgcgatag atatttcgat caacgatcag ctgatcgact tggcttcttt
    32881 ggaaccagaa gacttttact gggaattaga tatgaaaaat ggtgtattat ctcgtacatt
    32941 cacaatcact acttctacta ataaagtacg catttccttc gaacgttttc tgagcattgt
    33001 caaaaaagaa tcagcttata tctatttaac tatagaaatg cttgaaggta gcggaacagt
    33061 caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa gacagcaact atgatgaaca
    33121 tttttggaat gagattgatc gtggaactat agaagaaatc ggctatttga cgactaaaac
    33181 tattccaaac aactttggga ttgaacaatt tactgttact gcagcaatgc gccatttcgt
    33241 taatggaaaa tcagtcatcc ctgactatca cgaagatact ttagcattac atggttgctt
    33301 gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa aaagaaatta tcgttcttac
    33361 tagtagagat attccagaag cgcagcaagc agaaaaagct gtctcagaat taacgaacca
    33421 gacaaacaat tatatccaag caaaagaaga gcaaacagcg gcttgggcag aacgatggaa
    33481 gcttgccgat gtggtcatcg aaggagacga agaagcacaa caaggtattc gattcaattt
    33541 gttccaacta ttctctactt actacggcga agatgaacga ttgaatatcg gccccaaagg
    33601 attcacagga gaaaaatacg gcggagccac ttactgggat acagaagcgt atgctgtccc
    33661 tctttatctt gctttagcca aacctgaagt aactaagaat ctgctgaaat atcggcataa
    33721 tcaactgccg caagcaatcc acaacgctca gcaacaagga ttaaaaggcg cgctttatcc
    33781 tatggtcacc ttcactggtg tcgaatgcca taacgagtgg gaaatcacat tcgaggagat
    33841 tcaccgaaat ggcgcgattg cctatgcaat ctacaattat acgaattaca ccggcgatac
    33901 ggaatatttg aaaaatgaag gactggaagt tttagcagaa atcgcacgct tctgggcaga
    33961 ccgtgtccat ttttcaaaac gagcaaataa atacatgatc cacggtgtga ccggcccaaa
    34021 cgaatatgag aacaatatca ataacaattg gtatacaaat acgatcgcag cttgggtctt
    34081 gcgttacacg cgagaaaatt atttaactta tcaaaaagaa gctgctatca ctatcagcga
    34141 agaagaacta gccaaatgga aagatatcgc agagaacatg tacttcccag ttgacgaaga
    34201 attaggaatc tttgtccagc atgacacatt cttagataaa gatctcatgc ctgtatccga
    34261 tattcccaca agcgatcttc ctctgaatca gcattggtct tgggacaaaa tccttagatc
    34321 ttgcttcatc aagcaggcag atgtactaca ggggatttat ttcttcaatg atacttttac
    34381 aaaagaagaa aagcaaaaaa actttgattt ctatgaacca atgactgtcc atgagtcttc
    34441 cctttcgcca agtatccatt cgattttagc agcagaatta ggaatggaaa gcaaagctgt
    34501 agaaatgtac cagcgaacag ctcgcttgga tttggataat tacaataacg acacagaaga
    34561 cggcttgcac atcacttcca tgactggtag ctggttgtcg attgtccaag gatttgctca
    34621 aatgaaaact gcccatgatc agttgagatt cgcaccattt ctgccaaaaa cttggaaagc
    34681 ttattctttc catatcaatt atcgcggacg tttattgttc atctcagtca cagatgaaca
    34741 agttactttc gagttattag aaggttctcc tctagaaatt tacgtctatg aacagtctta
    34801 ttctttagcg gatcgtctag aaataacact aagaaaggag gaagcaaatg tttaaagggg
    34861 tattgtttga tttagacggt gtcattaccg atactgctga attccattat cgtgcttggc
    34921 gaaagcttgg tcaggaaatc ggcattacga tcgaccacac attcaacgaa cagctaaaag
    34981 gcgtcagccg agaagactca ctctctttgc ttttagctta tggcggaaaa gaacactctt
    35041 tttcaaagga agaatttgca gaattagcga aaagaaaaaa cgattattat ttagagatga
    35101 tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt gcctttactg gattctttaa
    35161 aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa aaacgggcca ttcctacttg
    35221 aaaaaatggg tcttacacct tactttgatg ctattgccga cccggcagaa gcaacaaacg
    35281 gaaaacctgc gccagatatc ttcctccttg cagcaaaagc tgtcggacta aaagcagaag
    35341 aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc catcctctca agtggcgctc
    35401 aaccaattgg cgtgggacgt gctgaagacc ttggagagga gatcccgctt gtccctgata
    35461 cgacttttct tacgatcgat tacttgaaag aaaagtggca tgaccatgga taatccagag
    35521 atctccgtcg taccagttcc agagactgaa ataaagaaaa tcacattgaa gaacgactac
    35581 ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc aaatcttcgc accagacaaa
    35641 gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt tcgaagatgt tctaacagac
    35701 aaaagctttt ttggagcaac tgtcggacct gtagccggaa ggatccggga tgcttcttgg
    35761 ggtgagcata gattggagaa aaactgcgga tcacaccata tccacggtgg cacgaaaggc
    35821 tggtctttcc aattttggga agtagaagtt ttcaaaactc catcatcaat aggtgtggtc
    35881 ttttacttaa aagatgaatt ttctacctat ccaggaccaa tcactgctac gatcacttac
    35941 cgtttaacaa aaaacgaatt agagatgaca acctctgcta gtagccttgt tgaaaccatc
    36001 tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa atggaacaag agacatctat
    36061 gaacatcagt taacggtctt tttagatggt gtgcttgaat tagatcaaga aaaactgcct
    36121 actggaaatt ggacaaaaaa agaaaaactg ccaatcgact ttagaaaatc cccaacactg
    36181 caagagatcc ttgcatgtta tccagatgga ctagatgatg tatttctttt gcatcatcct
    36241 aggctttcca aaacttcctt acaattattc gagaagcatt ctggtcgcca aatgactata
    36301 gcaacttcta ataaaagtat ggtattattt tctacgactg gattcgaagc tgatttctct
    36361 gtcaatggaa aacagatgca ttcaaactac ggattagcaa tcgaaccgca agaaatccct
    36421 gatatcgttc atttccccaa atttggatcg atcaacctcc atcctggtca agaacggatc
    36481 agtcagacga tttatcggtt ctctgcacaa taacaagaga aaggtgtttt tatcatggct
    36541 attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca cctcgaccgt ctctcgtgtc
    36601 atcaacgatc atcctagtat ttccgagaca acaaagaaga aagtccgaaa aattatggat
    36661 gaactaggtt atgttcctaa catgactgcc cgcaatctag gaaaacgcat ttccagtgcc
    36721 atcggcgtaa tcttaccccc tcttgattca aaagagcgga tcggtaaccc attttatctt
    36781 gagatcatgg aagcaatcaa cgaagaagcg cggaattatg atatgactac agcaatcgct
    36841 actgctaaaa attttgaatt gctgttagag aacgtccagc gtatgcattt acaaaaacaa
    36901 gtagatggtt ttattctagt gtattcagat agtcaagata ctgtcattga ttatttatac
    36961 caaaaccaca tccctttcac tttgatcgga cagccttatc atcatgagaa cgaaatcgtt
    37021 tatgtagata atgataacca gcttctaggt aaacaagcaa cagaattttt gatccagaat
    37081 ggacataaga atatcctgtt tgtgacaaac acgactcatg aaagtttgta tttcgaacga
    37141 tatttcggtt atcaaaaagc aatgatgatg gctggtcttc ctgcgttatc cagtgtagta
    37201 ctagagcatc cggaggatta tctgtccttt gagaaaacgt tgaaagaaaa cgaagccaca
    37261 gcattggtgg tcattgacga tatcttcgcc cttcgcacga tgcagttggc gcaaatgtat
    37321 ggctatgtca ttccagagac actctccgtc attagcttca acaactcgat cttctcaaca
    37381 ttaacacacc cttaccttac aagtatcgat atcgatatct ccgaattagg acgtgtcgct
    37441 atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca gtggcgtacg tttggtcatc
    37501 cctcatcgct tgatcaaaag agagacagtc gttcctttag aaaacccata ctaaatagct
    37561 gactcactaa atacgctgac catttatcct atggtcagct ttttatgtaa aagaagatac
    37621 tacttctaac acattgttta tctgtaaaaa agagcgtggg acaaaagtaa agaacacttt
    37681 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag
    37741 ctgaaattca caaaaattta aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt
    37801 aaacgcttct gtcccagcct cttttttcct tccttcaatt ttttacacgt cgacgattca
    37861 ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca
    37921 agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa tgtgccgctg actccccacg
    37981 gtacgagaga attgacggtc gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg
    38041 ataagccaac gtcatcaaaa gaacgcttga atgtttctcc tggaagaatg atggacagat
    38101 actgttctcc aacgatcaga ttgattccga tacagctcaa ggcagtaaac ccgactaaac
    38161 gagaaggtcg gtccatcttt tcttttaatt cttgaacgat ggtatcaacg atcagatatt
    38221 tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa actagcagaa cctaacatac
    38281 tcatgatccc gcctcgagaa agcagttcat ctagctttgg atcaggtgta tttgcgacat
    38341 aaccattcat caagatgtct gctgcttttc cgaccgtcaa acttttctca tggataaaag
    38401 aaagaacgag agcagctgtc gatcccgcta gtaatgtagg gatagccgga atttttttga
    38461 ttgccccccc aaataaaata actagtggaa tcaatgtcca aggagagatc caaaagctcg
    38521 ctttcatgtt ttggatcatt tcattgatac tagctagatc ggctgaatga ctgttatgtc
    38581 ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg gaaatccgta taacgcatac
    38641 cagcgatatg ttcgaataga ttaactcctc caatacccgc tgccaagttg gtcgtatctg
    38701 ataaaggcga gcagttattc cctaaaaagg caccggaaac gactgcgcct gtagtcattg
    38761 catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg
    38821 ttgtaaatga actgccgaca gtcactccga caattgcaca aacaagaaat gctgtcggta
    38881 aaaagaaacg cacagaaaca agtttaaatc cataaaccat aatcgtaggg atggttccag
    38941 ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc
    39001 ctggagtgat tcctttgatg atcccgtcat ggatctcatc ccatgtgaac tttttaaatc
    39061 tgccataaaa caacaaacac atgaaagctg caaggatcgg gatatgtggc gagagctgga
    39121 atccgatgat cagtactcct aggagtgcca acaacacgat caatacagcg atactttctt
    39181 ttaaagaaat atttttattc atcgtcgatc tcctatttca aatgaaaagg ccctgccatg
    39241 aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag
    39301 acttattttt ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct
    39361 tgaagtgatt gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata
    39421 atgcatcact caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga
    39481 tcagcatgca gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac
    39541 gttcgaattc tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca
    39601 cgatacaata aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat
    39661 ccattacacc ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat
    39721 ttctagatac gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt
    39781 cacgagctac ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa
    39841 gcacaccaat aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga
    39901 tttgacagtc aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta
    39961 cgacatttgc tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga
    40021 taaatacgcc ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac
    40081 tcatttcgat cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat
    40141 gttccaagtc agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta
    40201 gtccgacttt gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata
    40261 cccctagcca tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga
    40321 tcgtgatgat ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa
    40381 gttctacatt accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca
    40441 tacctgggaa catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca
    40501 gtgctgtttt tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac
    40561 cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca
    40621 atgtccaacc gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg
    40681 tcagaccgat aaaggtccca agacccattt tacttacttc agatttactc accgttgaca
    40741 ctctccttga ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga
    40801 aacaattcag cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa
    40861 agacaacctc aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa
    40921 acatgcgtca gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt
    40981 tgcttcgctt gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc
    41041 tttgattttt tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac
    41101 gctggcacgc aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag
    41161 ttggttcaca aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga
    41221 agttaagaac tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc
    41281 atggttcaat ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc
    41341 taccatattg aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt
    41401 aaatgacaag tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat
    41461 taatttacca tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc
    41521 tgctgttgcc cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc
    41581 tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc
    41641 tttgatgacg ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt
    41701 tacagattct gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc
    41761 tttgttttct gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat
    41821 gaagttgttg tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac
    41881 gtgtaagtag aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc
    41941 gataccgtcg attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg
    42001 tgttttttca gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata
    42061 gttgtggtca actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc
    42121 ttttagccat gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg
    42181 ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa
    42241 cagattcatg atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac
    42301 tagttcaggt gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca
    42361 aagtccttct aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata
    42421 gctcattaat tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga
    42481 agattcatag gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga
    42541 tggcatcaat gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac
    42601 ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt
    42661 gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat
    42721 gtagttttga cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat
    42781 ttggccgttc tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa
    42841 tgattcactc atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt
    42901 acacctgttc ttctattctg taaactaagt tttggcctga tttttaatca tattttaata
    42961 tgtatcaatt atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta
    43021 caaatgtaaa ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg
    43081 ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa
    43141 ttctaatcaa ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac
    43201 ttaccatcga attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg
    43261 ttaatactta tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt
    43321 tttgtggaaa caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc
    43381 attttaccga atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac
    43441 aatgcttgcg ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta
    43501 acttcttctg ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc
    43561 gctaagtctt cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca
    43621 tcgcgatcat cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga
    43681 tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc
    43741 aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct
    43801 aatccagctg tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat
    43861 gttttatgca gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat
    43921 gagattccta cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg
    43981 aagtttttac cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag
    44041 ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt
    44101 gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg
    44161 cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc
    44221 aattggaatc ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca
    44281 gttggatcaa cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt
    44341 tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt
    44401 ttactgacct ccgtgtagtt tttttatgaa agcaggaacc tgctagcatg tgtaattaat
    44461 tatttcttca ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg
    44521 aaatctcgcg gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt
    44581 aacgctgtcg cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat
    44641 gtactggttt gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt
    44701 cgatcttcgt cttgttatta ttaatatgtg tctatcatag agcgcaactt tttctttgtc
    44761 aacagaaaaa ggatattttt tttaaattcc tcattttttc ttttttgaat ccgctctaat
    44821 tccgaacatt ttattttcaa ttattgttaa aagaaataac tttttctgta ttttttctgc
    44881 aatttatcga gaagtcactt tactttgcct cttattcttt cttcgttaca ataaggaatg
    44941 ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta
    45001 ttttaggagc cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca
    45061 tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa
    45121 aagaaccgac agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct
    45181 acgattatga ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa
    45241 cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc
    45301 ctactcagat ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc
    45361 actctcaaca ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt
    45421 cgatcgaaca actttatcaa aatgattttg ttttagttaa tttgaatgat ttgatccaaa
    45481 aagacgaaca aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat
    45541 tggttctttc acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta
    45601 gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag
    45661 aagaaatcgg tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg
    45721 atttttctta tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag
    45781 gttatcgaac atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag
    45841 aagctaaaaa agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat
    45901 ggggtcactt gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt
    45961 ggcagcaaga agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg
    46021 cagacatcag cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga
    46081 gtcaaggttt cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag
    46141 gtacggattt ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt
    46201 tagacggaaa aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag
    46261 aagcacgcga atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt
    46321 ttatcgtcag tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca
    46381 ggacttctgt ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa
    46441 ttccttctac cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca
    46501 cttttccttc tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg
    46561 tatacgcatc catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca
    46621 aatcgatata aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg
    46681 caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta
    46741 aatcattcat aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt
    46801 taaaaatcgt cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag
    46861 cggttcctat gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct
    46921 cttgtgctct cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat
    46981 aatatatata gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca
    47041 agctagcaat tccttgttta tgaattcatt gacagaaatt tataaaaaga atattatgta
    47101 ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat
    47161 cttgtttagt atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag
    47221 tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg
    47281 tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga
    47341 ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag
    47401 atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct
    47461 ctttgtgggg aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa
    47521 aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa
    47581 aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg
    47641 ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa
    47701 cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt
    47761 ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa
    47821 cactcgatga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta
    47881 tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc
    47941 aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg
    48001 aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat
    48061 tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt
    48121 ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg
    48181 gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag
    48241 gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa
    48301 ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc
    48361 ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa
    48421 aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa
    48481 actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca
    48541 gttgtacgat gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg
    48601 ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac
    48661 tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc
    48721 ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaaa cctttaggct
    48781 ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt
    48841 tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca
    48901 ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc
    48961 gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt
    49021 tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg
    49081 acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca
    49141 cgatcatggt cgtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt
    49201 tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga
    49261 accaacttta ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga
    49321 ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg
    49381 atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg
    49441 gaattagtgg ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact
    49501 tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt
    49561 ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg
    49621 tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata
    49681 ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca
    49741 aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg
    49801 ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa
    49861 taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt
    49921 cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata
    49981 gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt
    50041 tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa
    50101 gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc
    50161 aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc
    50221 tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc
    50281 ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc
    50341 cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt
    50401 gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa
    50461 acagctattt ctaataaaga aacataactt cccttattcc aactgataat tttcttttat
    50521 catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc
    50581 ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata
    50641 tttacttttt
//
LOCUS       DACCWK010000008        50374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCWK010000008 DACCWK010000000
VERSION     DACCWK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.008"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1312)
                     /locus_tag="IVW65_000418"
     CDS             complement(<1..1312)
                     /locus_tag="IVW65_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7899344.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLY"
     gene            complement(1314..1982)
                     /locus_tag="IVW65_000419"
     CDS             complement(1314..1982)
                     /locus_tag="IVW65_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7899345.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2112..2696)
                     /locus_tag="IVW65_000420"
     CDS             complement(2112..2696)
                     /locus_tag="IVW65_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7899346.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3027..3260)
                     /locus_tag="IVW65_000421"
     CDS             complement(3027..3260)
                     /locus_tag="IVW65_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7899347.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3275..4225)
                     /locus_tag="IVW65_000422"
     CDS             complement(3275..4225)
                     /locus_tag="IVW65_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7899348.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4222..5064)
                     /locus_tag="IVW65_000423"
     CDS             complement(4222..5064)
                     /locus_tag="IVW65_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7899349.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5066..5785)
                     /locus_tag="IVW65_000424"
     CDS             complement(5066..5785)
                     /locus_tag="IVW65_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7899350.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6477..6635
                     /locus_tag="IVW65_000425"
     CDS             6477..6635
                     /locus_tag="IVW65_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7899351.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6707..7933
                     /locus_tag="IVW65_000426"
     CDS             6707..7933
                     /locus_tag="IVW65_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7899352.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8021..8413)
                     /gene="rpsI"
                     /locus_tag="IVW65_000427"
     CDS             complement(8021..8413)
                     /gene="rpsI"
                     /locus_tag="IVW65_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7899353.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8429..8872)
                     /gene="rplM"
                     /locus_tag="IVW65_000428"
     CDS             complement(8429..8872)
                     /gene="rplM"
                     /locus_tag="IVW65_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7899354.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9391..9858)
                     /locus_tag="IVW65_000429"
     CDS             complement(9391..9858)
                     /locus_tag="IVW65_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7899355.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9978..11516)
                     /locus_tag="IVW65_000430"
     CDS             complement(9978..11516)
                     /locus_tag="IVW65_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7899356.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11596..12282
                     /locus_tag="IVW65_000431"
     CDS             11596..12282
                     /locus_tag="IVW65_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7899357.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12483..13010)
                     /gene="msrA"
                     /locus_tag="IVW65_000432"
     CDS             complement(12483..13010)
                     /gene="msrA"
                     /locus_tag="IVW65_000432"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7899358.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13094..13771)
                     /gene="pgmB"
                     /locus_tag="IVW65_000433"
     CDS             complement(13094..13771)
                     /gene="pgmB"
                     /locus_tag="IVW65_000433"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7899359.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13768..14898)
                     /locus_tag="IVW65_000434"
     CDS             complement(13768..14898)
                     /locus_tag="IVW65_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7899360.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14898..17081)
                     /locus_tag="IVW65_000435"
     CDS             complement(14898..17081)
                     /locus_tag="IVW65_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7899361.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17078..18103)
                     /locus_tag="IVW65_000436"
     CDS             complement(17078..18103)
                     /locus_tag="IVW65_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7899362.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18148..18975)
                     /locus_tag="IVW65_000437"
     CDS             complement(18148..18975)
                     /locus_tag="IVW65_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7899363.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18972..20039)
                     /locus_tag="IVW65_000438"
     CDS             complement(18972..20039)
                     /locus_tag="IVW65_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7899364.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20065..20901)
                     /locus_tag="IVW65_000439"
     CDS             complement(20065..20901)
                     /locus_tag="IVW65_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7899365.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20898..21767)
                     /locus_tag="IVW65_000440"
     CDS             complement(20898..21767)
                     /locus_tag="IVW65_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7899366.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21837..23120)
                     /locus_tag="IVW65_000441"
     CDS             complement(21837..23120)
                     /locus_tag="IVW65_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7899367.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23134..24819)
                     /locus_tag="IVW65_000442"
     CDS             complement(23134..24819)
                     /locus_tag="IVW65_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7899368.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25094..26119
                     /locus_tag="IVW65_000443"
     CDS             25094..26119
                     /locus_tag="IVW65_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7899369.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26714..30367)
                     /gene="rpoC"
                     /locus_tag="IVW65_000444"
     CDS             complement(26714..30367)
                     /gene="rpoC"
                     /locus_tag="IVW65_000444"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7899370.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVARSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(30446..34063)
                     /gene="rpoB"
                     /locus_tag="IVW65_000445"
     CDS             complement(30446..34063)
                     /gene="rpoB"
                     /locus_tag="IVW65_000445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7899371.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34791..35759
                     /locus_tag="IVW65_000446"
     CDS             34791..35759
                     /locus_tag="IVW65_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7899372.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35777..36682
                     /locus_tag="IVW65_000447"
     CDS             35777..36682
                     /locus_tag="IVW65_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899373.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36679..37485
                     /locus_tag="IVW65_000448"
     CDS             36679..37485
                     /locus_tag="IVW65_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7899374.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37642..38610
                     /locus_tag="IVW65_000449"
     CDS             37642..38610
                     /locus_tag="IVW65_000449"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7899375.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38927..39451)
                     /locus_tag="IVW65_000450"
     CDS             complement(38927..39451)
                     /locus_tag="IVW65_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7899376.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39738..41549
                     /gene="scm"
                     /locus_tag="IVW65_000451"
     CDS             39738..41549
                     /gene="scm"
                     /locus_tag="IVW65_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7899377.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(41704..42690)
                     /locus_tag="IVW65_000452"
     CDS             complement(41704..42690)
                     /locus_tag="IVW65_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7899378.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(42713..43279)
                     /locus_tag="IVW65_000453"
     CDS             complement(42713..43279)
                     /locus_tag="IVW65_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7899379.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(43293..43709)
                     /locus_tag="IVW65_000454"
     CDS             complement(43293..43709)
                     /locus_tag="IVW65_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7899380.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(44166..46100)
                     /locus_tag="IVW65_000455"
     CDS             complement(44166..46100)
                     /locus_tag="IVW65_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7899381.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            46402..47337
                     /locus_tag="IVW65_000456"
     CDS             46402..47337
                     /locus_tag="IVW65_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7899382.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            47427..48530
                     /locus_tag="IVW65_000457"
     CDS             47427..48530
                     /locus_tag="IVW65_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7899383.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            48543..49496
                     /gene="menA"
                     /locus_tag="IVW65_000458"
     CDS             48543..49496
                     /gene="menA"
                     /locus_tag="IVW65_000458"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7899384.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT        14986 a        10371 c         9061 g        15956 t
ORIGIN      
        1 tataaagaaa ttccggatca tgagtagcta taactactgc acccttttct tttaagtctt
       61 taataaggtc agcaatcctt tccatattaa tataatccaa cccacttgta ggttcatcaa
      121 atagaaataa attagttttt gacaatgaag ctatagaaac caataatctt tgtttttctc
      181 caccggataa atcaaacggt aattgatctt tcattttata aagttccatt ttttttaaaa
      241 ttttttcttg ctgatcttta ctaactattt cattccccag ggctaactcg ctcctcactg
      301 attctgtgaa aaattggtaa tccatttctt gcattactaa aaacggcgat gacaaatttt
      361 ttttattttc gattatcttt cctttggtag ggcgaataat cttcatgagt attcttagta
      421 aggttgtttt ccccacaccg tttttcccta ccaaaccaat aacatctcca actttgacat
      481 caaaatttat gtcttttaga atattttcat aagtaatttc ttcaactttt aatacatctg
      541 cacttacctt ttcgatatag ggaacttcaa tcgaactatt aaaaatgtca aatttcctta
      601 atgcgttatt gttttcagtt tttaacaact gaatagttgt agtttttaca gttctatttt
      661 taacaaatag aacttgatca attaattctt tcaaatagta aaaacgatgt tcagctataa
      721 caatagtaaa tcctttattt tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt
      781 tgaagtccaa atttgaagac ggttcatcca atataattaa tttagagttt agcgataaac
      841 tacatccaat agaaattttc tgccgttcac cacttgatag cgaaaaaatg tttttatcta
      901 atatatttga aagatctaat aattcagcta attcattgat tcttttatcc atatgctctt
      961 ttgtaaatcc aaagttttcc atcgaaaata ctaactcact cgttgtattt gttgtaaaga
     1021 attgtcctct aggatcttga cttacgtagc caatattttt tgatatactt tcttgtgtta
     1081 aggattgtag cgagttattt tcaaataaaa tttccccaac caattttccg tcataaagtc
     1141 ttggaataag tccattcatt aactttaata gtgtggattt cccactccca gaagaaccag
     1201 taatcaatgt aagactgcct ttttttattg aaaaattaag gtcaacaagc gcttgttcct
     1261 taacatcctt atatgaaaaa gaaacattgt taaattcaaa caattctttc acctcaaatc
     1321 aaaatactta agaaaaaaat agtaacggaa aacaggatga tatagtctaa taagctcatg
     1381 tttgttttat agtaagaact tcttttcaca ggagattcta tcccctttgt tattgcatta
     1441 gcggtcattt cttcagcaat catagttgca cgtataagaa cgggaactaa cagaaattcc
     1501 aaatattgca gaggctgaaa aaaagacaag cgaatgtttc tcatttttaa agattcttta
     1561 atcattctta tttcagaacg ataagtcgga aagaatctga tcactactgt aatacttaac
     1621 attatttttt tcggtaaatg tattttttct aaagaggata ataattcatt gctagtatag
     1681 acactaacca acaaccatga gatcattacg accggcaaca ttcgaaatac aatatataag
     1741 gatgtcgcaa aaaatatagt aaaaaactct atcttgatag catcaataat tttcattact
     1801 aaaaacagaa ctgaataaat caagatgaaa taaatacatg tctttatttt acgaaacatc
     1861 aatactaaaa ctgtagcaat aatgaaacac cccacttgaa atatatttcc ctttcctaaa
     1921 aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt gatttttgac
     1981 actctttcac ctccaaaaaa taagatcgca ctgttatctt aagctgataa aaatgcaccg
     2041 ttattcagat aacagtgcga tcttatcaaa aagcataact gagaacaatt ctcaatgcaa
     2101 gcttttttta attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt
     2161 ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta
     2221 gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg
     2281 atcattccga tagtctttaa cttatctaga caacagactg tctttttcgt cacaaatgat
     2341 gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa aatcaaatcg
     2401 aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt gaagttctct
     2461 ctccaacttt tctctatatc tatattttct aagtttaaga ggtatttggt aaactcttca
     2521 ttatacatat taataagcgc attaatatgc ggctgaataa tttcttcaaa taacgcttcc
     2581 ttagaagtaa aatgtttata aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc
     2641 cttatcttag tttgctgaaa tccttctttt tcaaaatggc ttagtccact ttctattaac
     2701 ttttggtgag ttataatata atctctggtt tctatgggta cttcctccta ataagatgaa
     2761 ttaactttat tatcttacac aggacttgaa gtttcaatcg ttaacgttta ttactgaact
     2821 cattccttag agctaaacta atgagaaaga gcagaataac tgatacaaaa agcaatccta
     2881 caagaattaa tagactcatc taaatcattc accttttcta aaataatccg agaaagattt
     2941 aaaaaaatta tatccagcta ttaggataag tcctatcata atcactccaa tagccatttt
     3001 cttatcctcc ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc gtcaataaat
     3061 tttttccctt tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta
     3121 gccagcatct cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt
     3181 tttaatttct tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta
     3241 gtcgttttaa tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt
     3301 tatccaggtt ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa
     3361 ttgaatcagt aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca
     3421 ttggtctatc atccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt
     3481 tttcaactaa gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa
     3541 tgtaagcaga aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa
     3601 caatcatttt tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag
     3661 aatccaattt ttcaatatac ttatctagat tttctttata gaatttttta ttatcaggat
     3721 ctttttccgc taactgtttt tcaatatttt tagcgtaaat aataccgttt tctaaattta
     3781 accaagcatg aggatcttcc ttccctttct cactctgacc ctctaagtaa ataacatcta
     3841 tgccatcact tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt ttaactaatt
     3901 ttgtaaacca agcatttcct ccagtttcca agttaacacc gttataaaaa atcaaatctg
     3961 cttttgaagt tttttgaaca tcttcaggca aaggttcata ttcgtgggga tctttcccaa
     4021 taggtacgat actgtgtaaa tcaattttat cttttgctat attttcggta atatctgcta
     4081 aaatcgaatt agtaactact acttttaatt tttcgttcga agccacttta tctttgtctt
     4141 tactatttat tgaacatgcc ccgaatataa aaagagcaat caaaatcccc agacttccca
     4201 gtactgcttt ccattttctc atcataacga tctctccttc tttcttaaaa atttttgttt
     4261 tggtgagatt agaaaactaa ttacaaataa gaatgccgct gttaaaacaa tacttgatcc
     4321 tgctgctatg ttaaaactat aaccaataaa caatccaagc aatgatgaac ttgctccgac
     4381 tgttgcagaa ataaatatca tagttttcaa gctatttgca tataaatatg ctgtagctgc
     4441 tggtgtaatt aacattgcaa caattaatat agttcccaca ctttgcatag ctgttactgc
     4501 tactaaagtt aatagaacca tcaatagata atgataaaaa ctgacattca tccctattgc
     4561 ttttgccatt aatggatcaa aagaagtgat taataattct ttaaaaaata gaactactat
     4621 aaccaacact aaaaccgaaa cgccaattgt aatccactta tcaatatctt gtacagctaa
     4681 aatatttcca aataaaatat gaaataaatc cgttgagcta tttgcaactc ctataaggat
     4741 aactcctagt gctaaaaaag aactgaaagt aataccaatt gctgtatctc cttttattac
     4801 actattgctt tttataaaag taataatgat agaagctaat agaccaaata caatcgcacc
     4861 aataaaaaaa ttaataccta aaatataaga aattgctact cctggcaaaa cagcatgaga
     4921 aatagcatca cccataagag acattcctct taaaataata aaacaaccta ctgttccaga
     4981 aacaatccct ataactacag atgtaattag agcattctgt aaaaagtgaa agtcatacag
     5041 accattaata aaattttgaa gcatattatt ccccctctcc aataaatata gtatctccat
     5101 aggctttttt taaattttct tcattaaata cagattctac acttccgtga gcaattagat
     5161 ttcgattcaa aagaataata ttatcaaaat acttctttac cttgcttaaa tcatgatgca
     5221 caattagcac tgtttttccc tgatgtttca atttttttaa cgtatccata attattcgtt
     5281 cactaactaa gtctatccct acaaatggtt catctaaaaa tatgaaatcc gcattttgag
     5341 caagacatct tgctaaaagt acacgttgaa attgtcctcc agataattct cctatttgat
     5401 gattagaata ttttcccata tcgacctttt ccaaagcctt tgaaactctt tgccattctg
     5461 catttttaat tctttgaaaa actttcattc cggcatatgt tcccatagat acacattctt
     5521 ttacttttat tggaaaagta aaatcaatat cagctttttg ctctacatat gcaatcttca
     5581 ccagtttttt attgatatct tcattattaa tggagacctc tccctgatga gggataatat
     5641 ttaacattgc tttaagcaat gtggattttc cagcaccatt tggaccaata atacctgtta
     5701 aagcaccttc ttgtattttt aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa
     5761 cattaagctt tttcacttct aacataactt tctccttcta aatgataatg attatcacga
     5821 tcatatggta tacaaaaatt atattttagt caaccctaaa ttttatagtt tagatttttt
     5881 tctgttgctg aagattagaa tgacaaaact atttgaaact acgcgtatag taagtagctt
     5941 tcaaaatata taaaaaagga gcgttctcaa atgagaacat gctcctaaaa ctactttatt
     6001 ggcgtatgga aaaattatac ttgaagtgga tagcgggttg aataagttga taagcatcaa
     6061 aacatttata aaaattattc aagactatgt ctgttccata tacaaatatg aaaatcagta
     6121 aactccattt aacttttttc gtagtgtgtc tttttataca tgtttatcgt tattaatagt
     6181 gaataggaaa aaaactattt ttgatcattg acaattaaat acaatttttg aataacgatc
     6241 cctgctattc gagctagaaa ctcaagtacg ccatgacctg attagcgaca aatacttaga
     6301 gatttctttt tgttctggga aaacaaaatg ccatgacaat agcagcaaat aatgatactt
     6361 ccgtaaggct cgccgtaaac tggggagagg tgaaattcct atgtgcttac ccaaagcaat
     6421 tcaaaaattt cataactact attttaggag atgattttat agaatccaga gtctacctga
     6481 ataaaaaaca aacctgtgca tttctaggaa tcgataataa tactctagat aagtggattc
     6541 gtatatataa tttcccagta ataagagttg gtggtgtagt tcgctttgac gtagaagata
     6601 ttaaagactt tatgaataca tttaaaataa cttgaaattt tcttctcgcc gcatatacta
     6661 aaattagtga aatatactgc cttacttttt ttaaggagtg aggattttgc ctacaaagct
     6721 ttcaaatggg aaatataaaa ctaatctgag atatccaaaa aggtttaaag aaataacagg
     6781 aattgcatca gaaaagtatc aaaaaacatt tccaaatagg caattggcga ttaaagccga
     6841 aaatgatatg aagaagaaaa tagaaaaagt tttgagagag gagaatgcaa actcattaga
     6901 attaaaagga aaaataacgt ttaaaaaatt ttacgaatct aaatggcttc ctagatatga
     6961 attaggtcaa acaattagat caaacaggcc tcctagtgat ataaccatta gtaatacaaa
     7021 agatatattt cggttgcata ttttacctat gtttggtgaa tacgcaatga attatctaaa
     7081 tatcaatact gagataatat cagatgaatt aaccaaaaaa tcgaaagaat atgcaaatat
     7141 aaaaatcata aaaggatatg ttcgttccat gttcgatatt gcagaaatac taaattatat
     7201 cgaatttaat cgaactacta aaattattca aagtatcact gcacctaaaa aaaatgcttt
     7261 agaagaaaaa agaatacaag aaggtaagca agcactatca tcaaaagaac ttactaattg
     7321 gatagaagca gtaaatgatg attttaataa tcatttactt acttttcatg attacacact
     7381 ttttatgatt accctgtatc ttggtgatag aaaaagtgag acttacgctt tgcaatggaa
     7441 atatattgat tttgaaaaac aaacggtccg tcttaaacat actttagata aatatcaaag
     7501 aaaaaagttt acaaaaggtc gaaaagatac cgtcattcaa gtaccagaag ttgtaatgac
     7561 attgctaagt gaatggaaat cggtacaagc tgatcaattg ttgaaattaa aaattaagca
     7621 aacgttagat cagtatctct ttacttatac taaaccatct ggagaagtaa attgtcctgt
     7681 tcatgccgat tacctaaact atcgaatcaa ttcaattaaa agaaggcatc ctgacttagt
     7741 tcatttatct ccacacaaac tccgtcatac ttatgcaact atagctagac aaggtggagc
     7801 agatatgaat caaatttcaa atgctttaac acactcagat atttctacaa caaagatata
     7861 tgtgaacaca ccagatatag ttgataaggc tgtttttgaa gcttttcaga gaggattgaa
     7921 taagtgtgat tgatatgtgt tcttaaatgt gttcttaaaa acaaaagaag cactttccaa
     7981 ataattgaaa agtgccagaa acgttgatat ataaaggata ttaacgtttt gagaattgtg
     8041 aagctttacg tgctttctta agacctggtt ttttacgttc gaccatacgt gcgtcacgag
     8101 taagtaagcc agcacgttta agagctaaac ggaagtcagg atcaacttct agcaatgcac
     8161 gagcgatacc atgacggata gctcctgatt gtccagcgta tcctccgccg tttacgttaa
     8221 caaatacgtc atatgcgcct tttgtttcag taacagcgaa aggttgattg attacttcac
     8281 gcaagtcagc atgtggaatg tattcttcaa catctttttt atttacagta attttgccag
     8341 ttcctggtac tagacgtacg cgagcaactg cgtttttacg acggcctgtg ccgatatatt
     8401 gaacttgtgc caatgaaatt ccctcctatt agattaagtt tgtgatgtct aaaacttctg
     8461 gttgttgtgc tgcatgtgga tgttctgctc caccgtatac gttcaatttt gtaaattgtt
     8521 tgcggcctaa agtgttttta ggaagcatac cttttacaga agtttcgatc aaacgacgag
     8581 aatttttagc acgcaattcg ccagcagtga ttgatttcaa tcctcctggg tagttgctgt
     8641 gacggtaata aattttgtca gtcgcttttt tacctgttaa tttcactttg tctgcattga
     8701 tgacgatgac gaaatcgcca gtatccaaat gaggtgtgaa agttggttta tttttaccac
     8761 gtagtacaga tgcaacaact gttgaaagac gtcccaatgg aacatcagtt gcgtctacta
     8821 cataccattt acgttctact tcgcctggtt tggccatata tgttgtacgc acgttatttt
     8881 cctccaattt aagttctgat gtttgacata cacttcgagt ttccggggct ctttgtgggg
     8941 caaacaatac catttaacat attacccgct aatctagtca atgtcaacgt ttttctgctt
     9001 gattgaaaaa tttatatgaa actttacttc ttttctttct tttatataaa agggtcgttt
     9061 cctgtcaaaa tgaaaaacaa aaagaggctg tgacaaaagt cttgtcacaa ccccttattt
     9121 tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc cataaaacat
     9181 aagaagctgt tttttaaaat tgtttcttgt gactcggagc tgaacggcag gcttacaacc
     9241 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
     9301 tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg atttcacaac
     9361 ctctttttcg gtcaagttca acaacagttt ttacgcgtcg atctctggcg ctttgcccaa
     9421 atgagcttgg atcatccaga tacgtttttc tgtggctgtt ttgaattcga taaagatatc
     9481 ttgtgttgga tcgtcgcctt cttctccaga aacttcgatt cctttttggt aaagatctgc
     9541 tagataacga tagcctgcaa caagtgtttc caaacgttct ggaatagtac gatcccagcg
     9601 gccaatttca tcagggattt ttgtgttatc tgcaaattca cgcaatgtag aataaggtga
     9661 accgtctaat gtgatcaagc gttcggatac ttcatctaac tgagcgttca aatcatccat
     9721 gaactcatcc atcattgggt gcaatgtaag gaaacctggt cctctcatgt accaatgtgt
     9781 ttggtgaaca acaacagaca tttggcttag atctgcaact aattgattta atacttcttt
     9841 ggtttgttgg aatttcatct ttcttgcctc ctaacatttt cttacactta aaataatagt
     9901 agactttcaa tgaaaagtac aacaaaatca tttacctgag aacgattttc agttcgcttt
     9961 tttatccgta aagccaatca tttttcagaa tcagctaatt cttctcgcag ttgagcaaat
    10021 tgctgataag tctgtgctaa atgcttttgt gtcgaaacaa ataatggata gattttctga
    10081 tattttttat gatcagtttc ttgtactcca acttgctctt tcaattctat caattcctgt
    10141 gcttcactga tactttggat ctttccgatg cttctccagc ctaaaatcac agctcctaat
    10201 gcggatgctt ctgtgacagt agggaaaaca agcgggtaac ccagtacatc agccatcatc
    10261 tgacgaaaaa tcggagattg agcaaacccg cctgtagcac ggatctctgt gagctgattt
    10321 tcctggcaaa ctccttttaa aatccgataa aggttgagac agatcccttc tagaatcgcc
    10381 ttcatcattt ctttttctgt atgttgccgc tgtaaaccga taaaactgcc aaacgcatct
    10441 gcgtcccaaa gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag accattggca
    10501 cccgctggca caaaagcgat agccgaaagc agtgcatcat atggattttc tcctttttct
    10561 ttagcagatt gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa gatgccagca
    10621 ccattgctag tcgctcctcc cactacccaa tgtttctgat ccagaacata acaaaacgtc
    10681 tccatttctg gatggaactg aggctgagta gtaataaaac gaagtgctcc gctcgttcct
    10741 acggtcaaag cggcgatcgt atcaccaaaa gcacctaacc ctagattaga aagcggcccg
    10801 tctgcccctc ccaaaacaaa tgatatatca tttggcaaac ctagttctgc tgctgttttt
    10861 gatggtagac ctgttacttg ataggtggaa ggaaagactt gcggcaattg ctctattcga
    10921 atgttcaagt agtcaagcac ttcttctgcc cattggaatt catggatatc aaaatatcca
    10981 gttcctgaag ctgttccgta gtcgctgatc agttcgccaa acaatctata aaagatatag
    11041 tctttgatac cgacaaaata agcagttttt ttgaataatt caggttggct gttttgcagc
    11101 cacttgattt tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg ccgataaaat
    11161 tctttgccaa gcacggtata tttcagtttt tctgcttcgt ccgctgctcg gttatctgcc
    11221 caagtgatca ttcgagataa cggtgttttc tcttcatcca acaatatcag gccatgcatc
    11281 gcagaggaga aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt aattacttct
    11341 tttataacgg tgcagacacc ttgaaagatc aattcctgat cttgttctgc catcccttgt
    11401 gtttcttgga tcaaagggta gcgaaaataa gcggatgctg cttcttgggc ttcatctgtg
    11461 aaagcaactg ctttggtttg tgtggttccg atatctaatc cgattgaaac gttcattttt
    11521 tcacctcaaa ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc ttggttttga
    11581 aaagggggag atggcgtgtt actttatttt atcattggtt gctgtatcgg atcattttta
    11641 tgtttaaccg cccagcggat tccgttaggt cactctatta tttatcctag atctcattgt
    11701 gtcaaatgct gtcgttcgct ttcttggtat gaattgattc cgattctctc gatcatcgtc
    11761 caacgatttc gctgccgcta ttgcaggtgt cggttgccct tctactattt gttggcagaa
    11821 gccttatgtg gtggtctttt tgcctggttc tttacttttt cttccgcaag aaatttttcg
    11881 acgttcatat ggatgctttc ggctttgcta ttttcgttga tggatttatt ttacttcatg
    11941 gtccattctc acacgctatt ctgttcttgg gcaattttat ggattttttg gttgcaggcc
    12001 ggcgtcttcc aatggcaaag tgtgctgtta acgtttatcg taggcggtgt gtgccttcgc
    12061 tatggtcaat cctatctcgg agctggcgat actctgttga tgctttcttg gagtggtggc
    12121 ttgtctctag aagagttgct tcagatcttg tttcttgcta gtctgtttgg aatatccgtt
    12181 ttttctgttt actttatctg tcataaaaaa aagttggaaa agctgccttt cattcctttt
    12241 ttgagcagtg cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt tgtgacaaac
    12301 gacttgtcac aacctctgat atatagtgtt ctcttaccaa atccttgtga cttggagcta
    12361 ggcgacaggt tcacaacctc tccactaaat ggtgttcaat cagctgaccc tcagtattag
    12421 ctcaaaatgc tggttttaca acctctgata tacggtgttc cataagtagc tttttcggct
    12481 tcttatttct tgacgcccca atgtcgacga aggaaacctg cacgtccaga accttctcga
    12541 taaagattgt aatggagctt gtttttcttg taataatcct gatggtaatc ttctgcagga
    12601 taaaattcgc ttcttggttc gatttttgtc acgattggct tatcgaatcg cccgctgtct
    12661 tgcaatgctt gttttgaacg ctctgcaata tttcgttctt cttcatcatt atagtagatc
    12721 actggacgat aagaatcacc gcgatcagcg aactgtccta atgcgtcagt cggatcggtt
    12781 tgctgccaat agatttccac caactgatca taagaaataa cttctggatc atattcgatc
    12841 tctactgctt ctgtatgccc tgttgtgcct gttgttactt gttcataagt tggatttggt
    12901 acatgcccgc cagtataacc ggatgtcaca gaaatgatcc ctggctgctc ttcaaatggt
    12961 ttgaccatac accaaaagca tccgcctgcg aaaactgctt tttttgtcat ataaaatccc
    13021 ccttttttct catcatacct tgatttccaa aaggaggata acgttttgct tgtgacagaa
    13081 aaaacggaaa ctattatata cgtgatacgg cttctgataa atgccaaaac gaacgtactt
    13141 gatcaaaaac ggttaaatca ggaatcacat attcagcaga aggaagattt atgttttcac
    13201 ctattcccaa tacatgcatg ttaccagcca aagctgcttg acaaccagct tctgaatctt
    13261 caatgaccaa gcatgcattt ggcggtacat tcaattgctg tgctcctttg agaaaaactt
    13321 ctggatcagg ttttgctttt gatacttgcg tgccgtcaat aaggacatca aaataacttg
    13381 tcaaattcgt tttttctaaa atgaggcgtg catttttgct tgctgatccc aatccgatct
    13441 tgatgtttgt ttttttcaag tactcaagaa cagctattgc accaggcaaa acggatgttt
    13501 catccatttt ttgaatcgct tgaagatata gattgttttt ttgctcagca aaggctgatt
    13561 tctctttttc tgagaatttc tgttccattt ttccaaatga cagcaatcgc tctagtgact
    13621 cggttctgga tattcctttc agttgctcgt tttctttctc agtgaatgga atggacagct
    13681 ggttagctaa aaccagccac gcttcgtaat gataacgagc tgtgtctaca agaacgccat
    13741 ctaaatcgaa tagtgctgct ttgattttca tcgattacct ccttgaaaat gaacgacttg
    13801 aagtaaagat gcgatgtttt tagctgtgcg atgaagatta tcagtggctt gctgtaaagc
    13861 ttcttgtaaa ggcacagctg caggaaggat cggaaaaacg gccgaaatgc caacatctgc
    13921 tgctttttcg cttcctttac caacacttcc gcaaatagct atcacaggaa cgccctctgg
    13981 tactcttcta gcaatcccaa ctggcgcttt tccttttaga ctttgtccat ctagacgtcc
    14041 ttcgcctacg attacgaggt ctgcttgctg tacccgttta tcgaactcca gtaaatccaa
    14101 tacgtagtcg atgccagaga ctattttccc tgaagcaaat gctaacagtc ctgcacccat
    14161 tccaccacca gcactactgc ccgatgcctc gattacttga ggggcaaatc tcatataaaa
    14221 atcatgcatg gcttggtcag ccactgctaa ttcattttct ttcaagcctt tttgtggtcc
    14281 gaaaacaaaa gaagcgcctt cttttccgca taaaagatta gtaacatcag taatgacagt
    14341 gattttggct ttaatcagct caataggcac gttcgtttta tcaactgtag caattttacc
    14401 tagattttct cccagtgctt ctactttttg attatcttga tcgtaaaaag cataacctag
    14461 accttctgcc atgccgatgc cgccatcgtt ggtactactt ccgccaacgc cgatgatgat
    14521 gtgtcgaaca ccttgttgca acaattgtcg tatcagttct cctactcctc gcgtggataa
    14581 cctaagtggc tgttgttggt ttagtgggac atctttcaat ccgacgattt ctgccatttc
    14641 aaaaatcgca gtttgaccgt ctgaagcgta ctggatttct ttgtttttgc caaatgcatt
    14701 agtaacgact gctgtttgcc gcttcaacct taaactgtct gctaatgatg aaaccgttcc
    14761 ttctcctcca tcgcctatag gtagcagatc atattcagcc tgaacaaaaa cttcttgaaa
    14821 acctgattga atggcttgtg ctgcctctaa ggctgtcaga ctttctttga aagaatctgg
    14881 cgcaatcaga atacgcatta ttttcccctc cgttccaaga tcagttcttt ttccagctgg
    14941 tattggtctc cattgacaaa aataggtaat gtttctttcg tctctttttt tattactatt
    15001 tgtttttttg tcaaaacaaa tagcaacctg tgatttttcc aaataaaagg aaaagaaatt
    15061 ttttcccatg ctgaaggaag tcttggagaa atagaaagag ttccattcgt tactgtaata
    15121 cctgaaaaac caaatataac tgctagccat agtgctccta atgaagcagc atgaattccg
    15181 tcatctgatg aatgcggatc tggtcctaaa tcaatcaggc acgcttcttg gaataaatga
    15241 tacgcccatt ccatttcttg gatacggcaa gcctctatgg cgtgaatcgc tttactcaac
    15301 gatgagtcgt ggattgtttt gttttcatag taatgcaaat tcttagaaac gattggttga
    15361 gaaaaatact caggtaataa gtataacagc atgacaagat ctgcttgctt gctgacttgg
    15421 agttcattta cttcgctacg ggcataatca agtaaaatag actggctgcc aggacgttct
    15481 ttgtaagtgg tcaaatcaat aactggctta ctgagatacg tgtcatcttg cggaatcagc
    15541 tgttctttgt taggcactgg caaatataac ttatctaaaa aattttgaca ttctttcaaa
    15601 aaaagcggat cgccagcttg atatttaaca gctaaggcca cattataatg cgccagatag
    15661 ttcgtatatg cattattgtc gatatattct gtgtattcat ctggaccaat gactctgtgt
    15721 atctctaaat gatcatcgac ttttacagct ctgctcagcc aaaaagctgc tgtttcatga
    15781 atgagcgttt ttccctttgt ttctaaaaac gattgatcct ctgttgcttg gacatactct
    15841 tgaacggcat aagcaaggtc tgcactgata tgatgttctg cggcagctga agcaacttct
    15901 tgtcttgttc cggttttgat attcaatgca gcaaatttgg gtgtctcttc ctgaccagtg
    15961 tgtgcgcttt cccatgggaa aagtgcacct tgatagtgat aatctcttgc tttttgccgt
    16021 gcgccttcta atcgatcata acggtacagc agcaaattct ttgctacttc aggaaactga
    16081 tataaaaaga aaggcaggat aaaaatttcc gtatcccaga aaacatgccc tttataacct
    16141 tcgccagtta gtcccttcgc ccctacacta acacttggat cattgcgggg agtcataatc
    16201 tgtaaatggt aagcagcgaa gtccatcgct aactgatcga aagaagaatc gccttggatc
    16261 gttatccgat gctgatgcca aaaatctgtc caataagaca tcgactgctc taaaatttct
    16321 gtataactaa cgcttttctg ggcttgggca atacttttct taaacgggtc tgtttgctga
    16381 agagaggtag agagcacgat gatcttctcc agcgtaaagg acgtattttt tctgatcttt
    16441 tctgaaaagg aagcggtgat ccggcgattt tttgcttgga atacacctgc tttattgaat
    16501 ttagctgcaa tcccaatatc aattttactt tctgttgtct ggtaggatgc actgatcaaa
    16561 tcgtcaccaa atactcgcag ttctttttca accaactgct gtgtaccaaa attggtttgc
    16621 tgtccatcga tacccgtttt cacatctact tggcagtctt cggtcaacgg cttgatgacc
    16681 actttcaggg ctgcctgatg gagattattt tgatcagcaa cacgacaagt aatgacttcc
    16741 actttttttc cttgagcagt ttcaatgagc gcatagcgtt taagctctcc catttctaaa
    16801 tctagtgccc gctcgtatgc aagaactttc atcgtctcaa gagaaaaatg ttcaccgtcg
    16861 atttttattt cccatctgat gacatctggg agattcacta actcggctga ttctgctcct
    16921 aaaggtcggt tatatacacc agctgcaaac attcccctta cttgattccg atagtcttcc
    16981 tcctggcttc ctctgatacc aaaaaatcca tttccaatgg tcatctgact cgcatactta
    17041 ttgatatact ctaaatcaaa ctgtttttct tctactttca taaggtaata acttctcctg
    17101 tttctgccga acgatataaa ttttccacca gattctggat gacatatcct tgccagccat
    17161 ctgcaatcat ctgagtctgg tcaccgagcg cccgattcac aaaatgagcc atacttagtt
    17221 catgtgggtc attaactggc gtggtctttt gtaaggaaac cagctttcca tctacatctt
    17281 cgaatagttc gcctggatac agattagcac ctgcctgatc gccacaaaaa gccacattca
    17341 gtttgtcttc tctgatattc aaagcaaacg aagtattcag ttggatgatc ttgccgtttt
    17401 ctaattccag tgtgccaaaa attgcatctt ctactgtgaa tttgcttgga tcccatttcc
    17461 cgaatttccc tgaatttttt ttcgtaccaa ttttttggaa gctgtacgcc cgaacgcttt
    17521 ttactttagg aaatccagta atatacatcg ctgtatcaag catgtgaatg ccataatcga
    17581 tcaaaggccc gcctccttga agttctttgt cgataaacgt tccccagcct ggaacaccag
    17641 accgacgaag cgcattcgct tctacgtaat aaatatcccc gagaacatca agatgattct
    17701 tgatcattgt tgcttcgtca gaaaaacgat gctggaatcc ataagccaaa atctgcccgt
    17761 ttttatcggc tgtctctgcc atctttcttg cttgatcagc tgtcatagct ggtggtttct
    17821 cgcacatcac tgaagcatta gcttcgaggg cagccaatac atgttcgaag tgaaagcgat
    17881 tgggtgtaca gacactcacg atatcgggag aaatctcatt cagcatttgt gtaactgatg
    17941 tatacgcatg agaaaatcct gtacgtcggc aaaagtcttc tgctcgctgg ctgactggat
    18001 caaccacagc ggccaattct aagacatctg atcttttttg ataataagca gcatgtactt
    18061 tttctgctac ttgtccagcg cctatgattg ctactttctt catttatttc tccctccttt
    18121 gcttatttac ttattgttat tctttctcta ttctttctct aaatcattta aatgacgcgc
    18181 tgtttccaga cagtttttca agtattgcaa tgattcctga taaattttca cttcattttc
    18241 tcctcgcaaa cgacattcat ataccatata accttcatac tcattttgaa gtagcgtttg
    18301 aaaatggcga gtaaaatcaa ttgcgccaga tcctggttgg tagcgatgat tatctgctaa
    18361 atggatatga cccacatact ctttttgcgt agtcaatgct tttgaaatat catcttcttc
    18421 aatattcata tgataaaaat cagcgatgat ctgcacatgt ttcaattgat tagagtcaat
    18481 gtaatgacgc gcatccgcta acaaattgat catatggtct tggtatcggt tcaaaggctc
    18541 caaataaata accgtacctg tttcttcggc tactttatct aaataacgaa gtgattcgct
    18601 gactgcttta tagtctcctt ctttgcttcg gggtgaaacc atcggcggta atcgataagt
    18661 gaacattccc caagctgctg ggataacgat tcctttgcct ccaacctcgc ttagtgcacg
    18721 caaaatctca gcaatttgac gtacaccatt aagacgccgt tcttcgataa agtcaccgat
    18781 ccacccatcg tagccgttac aagctgtcac tactgggatc cctgtttctt tgattgcctg
    18841 tttgactgtg tcgatatttt ctattaacaa cttaccgtcg atttcgtatt cttcaaatcc
    18901 acagtctttg ataaatctaa atttttctaa aatgtctctt gggaaaaatg cttgatcttg
    18961 cgtaccgaat ttcattgttc tcctcctcgg acttgaacgc ccattttgat actttcttct
    19021 gggtgctggt ctacataacg catgtaagct tctgcgcttt ctgcaaatgg cacaactgga
    19081 tcaatgattt cttcacaatc caagtaaccg ttcatcaata attcccaaca tgtgtcctcg
    19141 attcgtttac ggttccatct tgggtaatct gggtttggtt cactagatgc tcgagcaaag
    19201 acgatttttg cattattgaa atgtgcttcg cgaccaagat taaacccagc tggaaaaggt
    19261 ttagcaaagg caacataaga aatgatcccg ccatagccga tccctcttaa agaagcttgt
    19321 aatgcggcag cactgccact cgtttcaata actgaatcta ctccacgttt atctgtcaat
    19381 tcctttagac gaaaaccgac atctgctttc ataggatcta gcgtgtaatc ggcaccgtat
    19441 ttttctgcta attctctacg ctgcgctatg ggatcgatcc caacgatggt agtagcacct
    19501 gcttttttag ctaattgaat cgcaatttgc ccgatagcgc ctaaaccgaa gattgctaca
    19561 gcatcacctg ctcgaacatg tgcttcccgg ataccaccta aagcgaattg agcaggatcg
    19621 taacatactg catttttcca ttgatccaac cgatcaagtt tacgtagttt ataattatca
    19681 acaccgtttg taatcagcgt ttcacgaatg ggtccataag tgcagactaa ctctcctttt
    19741 tgatactctg tcaccaaact gcccgtttct tcgatcgtac cgacaatcat atttcctaat
    19801 tgaaattcgc caaataccac accgcgttgg cttccttctt ctcttggcaa gaataggcgc
    19861 cattcttctg aaaattcttc atcgataaat ggacttaatc ctctaaagtc gacaatttct
    19921 gttcctggtt ttggagtagc gaaaattact cggatacgta cttcgttctt ttcgatctca
    19981 cggtcttcgt aatctttgaa tccggctatt cttggttcta atgcaactaa ttttttcatc
    20041 ttttctttcc tctttctttt ctttttatcc ttttacgccg ccttcgacta aatcaccttt
    20101 aataaatcgt tcagataacg catacataat gatgactggt aaagcggtca ccaatgaagc
    20161 ggccatcatt cgtccccaaa tgtagtctgg tgtactgaat aatgtgttca aaccgattgg
    20221 taaagtaaat ttttcggagg tcgacaggaa aatcgaagca aataaataat cattccacga
    20281 aaccataaag caataaacga agacagaaat gattccagaa atagccaatg gtataatgat
    20341 ccgaataatg atctggatcc ggctaagacc atcgatcatt gctgcttctt caatatcagt
    20401 cggaatcgtg tcaaaataac tttttagcat gaatatcgca gtaggaagtg tttgaacgac
    20461 catcgtgata atcagtgctg tattggtatc atacaaacct agaccggaaa tgattttaaa
    20521 taatggaaca acgatcaaaa taccggaaaa catataaacg gtgtaaaagc tagcattgat
    20581 tttcgagcga gctttgaagc gtagttttga caaagcgtat gccccgctga tcccaatcac
    20641 tactgcagtt acggaggcta tcaaggagat cactaaacta tttttgaaat acatgatgaa
    20701 tggaaaaatc tcagcattga aaatatcctg ataatgttca aaagtccatt ctttcggaaa
    20761 aatagtttgt gtcgttgcaa ttgcttcttg tgaatccttg aatgatgtca tcagcatgat
    20821 caaaaatgga aacaaattga cagctaatag caacaaaatc gtcgcgtaaa ataagatttt
    20881 cctgctcttt ttatttttca tttcaagtcc accttctttc gtgagagcaa gacgaccgct
    20941 gcaataatga tgaacaatag aatcgatata gctgctgctt ttccaagatc attgaacgca
    21001 aaggctgtct gatacaaata aacgcctaag atattgactt tattcgtcag tagatagaca
    21061 tctgtaaaca tatagaacat ccagatagat cgtaaaacaa tcacagttgc tagtactggt
    21121 ttgattgccg gcagtgtcac gatcttgaac ttctgccaag catttgcacc gtccatttca
    21181 gctgcttcat aaagcgtttt gtcgactgtt tgcaaaatag ccaaaaatga aataaacgca
    21241 tacggatagt atctccagat agcaaacaaa acgaccataa agaagctagc aactggctgg
    21301 tcgaaccaca gcggtgcttc gttgaaaagc tgcaacctat ctactaacag atcattgacg
    21361 attccataac tattattgaa catatatttc caagcaaaaa tcaaagaaat agatggcgta
    21421 acataactta ataaaatcag tgaacgcacc gttttcctaa atctgaactt gcggttaagc
    21481 aaaatagcta cggctaatcc taaagctgta ctgcctgcta caactaagaa tgtgtaagca
    21541 actgttaaac ctaaagaacg ataaaattca ctatcactta aaatttcaag ataattgctt
    21601 aatccgataa aaacggcgtc tgagttagga ttcagcggca tatcgaaaaa actgatctga
    21661 atattggaaa gcattggaat cagcactaaa acagctaata aaagcatgct tggtgctaaa
    21721 agtcccatgg ctaattttaa gtcagaatgt ttccttggac gattcattga aacctccccc
    21781 tgataatctg ccaatagcta tctacttttt tcgaacacca ttggcagaac ttcttgttat
    21841 ttcgtatttt tttgtgcgtc agctaaagct tgatcgacat cttcgtttcc gacagttact
    21901 tggttcactg cttttccgat cacacctgag cttgtgatat ctcccatttt cgtgaaattc
    21961 ttttccccga ctagtccaaa gacttgtact tcatcaaaag cactagcaat ctcagatggc
    22021 agctcgccaa aagacttgat gacttcattt tctttgtaac catccagttc gactacttgt
    22081 ttattcactg gttgagcacc accaggagac atcaagaccc acttttccat gtttttcggt
    22141 tgagataaga aagctgtgaa tgcttcagca gcctcttttt gtttctcgtc tagaccagaa
    22201 gaaatcgtca atccagatac tgtgccatag acagcttgat ctttttgtgt tgggatagca
    22261 aaaccgatat tttctgagtt ccccgcttca taaacagaag gcaaaatgta agttgaatag
    22321 attgccattg gtgctgttcc attcatgaaa gcatctttga tttctgtcac atcattagag
    22381 cctggcatcg tgtattggga taagttttga taaaacgaca atgcttctct catcttttcc
    22441 gtatcgatcg ttacctcgcc ttcatcatcc aaaacatttg ctccatttga caaagcaaat
    22501 tgagagaacg cttgctcact cattgttcct tcagcagtag gcatagcaat tccatattgt
    22561 ttattctctt tatctgtgaa atgttgagca atttttaaaa tatcatccca attttctggt
    22621 tctgaaaaac cagcttctga taatttttct ttgtcatacc aaataccttg aacccagccg
    22681 ctaattggtg cagcaatata gctttttcca tcttctgaac gaacaaggtt tttggctccg
    22741 tcataataat tatcttctcc agcttcctcg ataacgtttt ggactgccgt ctgatcaatc
    22801 agttcatctt tatccatcac tttagcaaaa tcctgactga cttccattac ttctggtaat
    22861 ttaccggaac gagctaacgt aacgattttt gtattgaacg ctgattcttc taccggcact
    22921 tgcttgattt tgatgtctgg attttctttt tcaaaatcag caaccagttc attgatcaca
    22981 cttaatcgtt cctgttcgac agaagagtgc atgaattgaa tcgttgtctg ttgtgattcg
    23041 ttagagcttt gactttcacc acaagcagta agaagaaatc cagtagctag cagacaggta
    23101 gtaacaataa tttttttcat ttttttccac ctcttatttt attattttca gccacatata
    23161 ttcatacggc tggaatatca actttcgttc ttgactgcaa tggataattt gtccagaaaa
    23221 caggtctagg taatctcctt ttggtaaaag ataacctgct gattgattgg aaaaattatg
    23281 gataactaag actttctctt ccttcgcttc gcgaataaaa ctgataaaag aatctgctga
    23341 gtccagtaag taaatcaaac tattcggatg gaaggctttt tctttttttc gaatagatag
    23401 catctgggtc aattcaccat atactgctgt tcttaatgga tctgtctgca actctttctc
    23461 aatatctgct aacaaaaatt tttgccgatt gatttttcga ttctctcctg ttttttctac
    23521 gcctttgtaa tcatttcgac ttcccagcac actttgaata taaattgccg gcacgcctgg
    23581 taacgttacc aaaaatgaat gagcagctaa aaagcgtttg cttctccatt ccgtcgtatc
    23641 tgttggagaa cttaacgcat ctagatacgt agcgtttatc tcatatggac tggtcgttcc
    23701 atctgggttt ttcttgtagt ttactcgggt accctctttc ttcagctgtt ccactaattc
    23761 aaggatcgtt ttttccggca gaatacctcg taccggattc aatccgatac cgtcatggga
    23821 ggctaaaaaa ttaaaaaaag ttgttttttt cgatgcgaaa tcgatggttt ttaaccattc
    23881 tttcaactta cttgtattac ccgaacgaat cgcttgaagg gtcaaaggtg gtagcgaaaa
    23941 ctgatagacc atctgtgctt cattctcgcc attcccaaaa taactgatat tatctttgtg
    24001 tggcacattt gtctcagtga tcaataccgt tcctggagct gcttcatcag cgattgcacg
    24061 gaataattgg atgattttat gcgtttctgg caggtgtata caagaagttc ctagttcttt
    24121 ccataaaaat cccacagcgt ccaagcgaat aaatgaagcg cctttttcta agtaaaacaa
    24181 aagaatatcg atcattttca ataaaacttc tggattttta aaattcaagt ctacttgatc
    24241 ttcgctaaaa gtcgtccata cgtgtttttc tttcccgttt ttgtcaatga acgttgtaag
    24301 taacgggctg gtccttggtc tagtcacgct gcttgtatct gtatccagtg gcaaatcaat
    24361 aaaaaaatct ttgtacgtat cattcaagtt taaatattcc tgaaaccaag tactttcggc
    24421 agacatatga ttgcagacaa agtcgaacat taaccgtgtt tcgcttctca tttcttctat
    24481 atcttcccat gtgcccaaac ggtcgttgat tgcctgataa tttacaacgg agaaaccatc
    24541 atcagatgta tatgggaaaa atggcaagaa atgtacaact ctaaatgtat ctgccaaata
    24601 cttttgataa aatttcttaa atgtacgcaa agttggttgc tgctcttcaa aaaattgatc
    24661 cccataagta atcaagaaaa tatcttcttc gtcccaatgc agtttgcctg cctggccttt
    24721 cttctgctga taaacactga ctttttgttc aatcatagag gcggttttct cagacaaatc
    24781 tgagtcgcga taaatcgctt gtaaaagttt tgtatacatc ggattcccct ttcttttttg
    24841 ggagcgttcc caacgatttg atcatattat ggaaaacgct tactgtcaat agcatttttt
    24901 taaataattt tgatatacac aaaaatacaa ataagaaagc ttatgtatca ataattaata
    24961 gcttcttaaa aaaatatggt ctctcatgat catgcaagct attcctaaat tttcaaaatc
    25021 atagacaaaa aagaatgtct attgtactat ggaattattc taaaacgctc ccaaaaagga
    25081 ggtgcatata aaattgaaac ctactattta tgacattgcg aaaaaagcta atgtctcaaa
    25141 atcaactgtc tccagagtat taaataacca gccaaatatc tctgataaag caaaagcaag
    25201 tgtacttcaa gcaatcaaag aattgaatta tgaaccgagc aaaattgcaa gaggactgtc
    25261 aggaaatggt tttgacgcga ttctagcatt atctcatcgt acttctcata cgacctatgg
    25321 caatcctttt ttttcagatg ttattcaatc catttctgcc gtttctgaac aaaataactt
    25381 cgacttgatt ctacaaacgg caaaaacacc agaagaagag ctagaaaaat gcgcgaaaaa
    25441 gatccaagaa aaaatgatcc gaggaattat tattttaagc tctacgatgg atgaaacact
    25501 tctttttgaa ttggatcaat acaatattcc tattgtggta atcggaaaag tcggaaacga
    25561 ctacacgaac atctactcag tggacacgga taatttccag gatagttacg atttggtgga
    25621 aacactgatc aagaatggac accgtcatat cggctgtctc catgctccga cagaagttta
    25681 tgtatccaat gatcgtctca atggttaccg tcaatgctta tttgatcacc acctagatat
    25741 ccgtaatgaa tggattatag acggcggctt tcgcttagaa gatagcatgg cagcaatgaa
    25801 tgatttaatg aagctacgag aaagaccaac tgcgattttt tctaccgatg cattaaaatc
    25861 attgagtatt tacaaagtgg cagaacaatc tggaaagcag atacctgatg atctttcgat
    25921 cgcaggattc agtgatccct tattgtcctc cctattttcg ccacagttga ctcggataga
    25981 cattccgaca aatcagttag gaatcaaagc cacagaactt ttattccaac taatccacca
    26041 ccaataccct gaagaaaaaa gaatcattct ttcaaccgag attactctca cagattcgat
    26101 ccaacaacaa gaaatgtaga acagaattat aaaagaacag aaacactgag gataagtcag
    26161 ttactctgtt cttttttcaa tcactcatcc ctcgttaccc cctcccggta ttttagcgaa
    26221 tttttcttat tttccatctg ttttagatag ctgtggataa ctataatgtt tgtgttaaac
    26281 tattttttcc acagggaaga aaaaactatt tttaaccacg ttgtgtataa gtttttatag
    26341 ttcgtttttt taaacacatt tacccttatt tcaatccatg ttatttgggg atacttatct
    26401 ctaaaacata aatagaataa cagacatgtc tactggtttt gttttattcg aggaattatt
    26461 caataatagc tagcaaaata ttaaaaataa ccgctctatc ttcccatcaa tttattttgc
    26521 attcaaaaaa tgatctatct ttacttttgt tgattgactt ctcttggaag atgatcgttc
    26581 cgatacctta attggatacg aaccgatgtt gttaagatcc tttcccactt ttcttctagt
    26641 tagacaaatt attttagaca aaccaaaaaa gaggttgtga caataatttg tcacaacctc
    26701 ttttttaaaa tatctatttt gtttgattgt tcattgcgtc ttctgcagcc atttgcgctt
    26761 caatatctga aatactgtag acattttcgc ttgctacacc gacttctttt ggttccatgt
    26821 cacgataacg ttgcatacca gtaccagctg ggatgatctt acctataata acattttctt
    26881 tcaaaccaag aagaggatct ttcttaccgc ggatcgcagc atccgtcaag acacgagttg
    26941 tttcttggaa tgatgcagca gataagaaac tatttgtttc taatgatgct tttgtgatac
    27001 ctagtaagac aggtcgagaa gttgcaggaa cgcctcctga gactagcgta ttgtagtttt
    27061 gatctttaaa gtctgcgatg tctaataatg tacctggtaa gatatctgta tcacctggat
    27121 ccattacacg aactttacgc agcatttgac gaaccattac ttcgatatgc ttgtcgccga
    27181 tttctacccc ttgcatacgg taaacacgtt gtacttcacg aagcaagtag ttttcaacag
    27241 ataatacatc gcgaacttgc aacaattgtt ttggatcgat tgatccttct gtcaatggtg
    27301 caccacggtg gatcatatcg ccttctgcta ctttcatacg tgctgtataa ggaacagtat
    27361 acgtacgtgt atctgttttt cctttgattg ttacttcttt ggtacgtgtt gcaggatctt
    27421 ctgagatatc gatgacatcc cctgttactt ctgtgatcac tgcttgccct ttcggattac
    27481 gtgcttcaaa gatttcttgg acacgaggaa gaccttgagt gatatcgtct ccggcaaccc
    27541 cacctgtatg gaatgtacgc atcgtcaact gagtaccagg ttcaccgatt gattgagcgg
    27601 cgatcgtacc aactgcttct ccgacttcaa cgtcagagcc agttgcaagg ttgcgtccgt
    27661 aacaatgttt gcagacacca tgttttgtat tacatgtgaa gacagaacgg attgtcactg
    27721 tttcgacacc tgcatcaacg atttgttttg cgatgtcttc ggaaatcaat tggtttcctg
    27781 caatgatcaa ttcatttgtt tcaggatgac gtacttcttt acgggtgtaa cgtcccacta
    27841 aacgatcttc taatgattcg atgatttcat ttccctcgcg gatggcttgg atttcaagac
    27901 ctcggtcagt accgcaatct tcttcacgga tgattacatc ttgggccacg tcaaccagac
    27961 gacgagtcaa gtaacctgaa tcggcagtct tcaaggctgt atcggtcatc cctttacgag
    28021 ccccgtgagt tgagataaac atttccaaga cggatagtcc ttcacggaag ttcgaaatga
    28081 ttggcaattc catgattcga ccattaggag cggccatcaa accacgcatt ccggcaagct
    28141 gtgtaaagtt ggaaatgtta ccacgagctc cagaatcaga catcatgaag attggatttt
    28201 tcgcttccaa accttccatc agtttttgtt ggatgctgtc ttttgcagca ttccaaacag
    28261 caatgacacg ttcataacgt tcgtcatctg tgatcagacc acgacggaat tgttttgtaa
    28321 tagtttcaac ttgtttatgg gcattttcaa tgatttcttg tttttcattc agtacactga
    28381 tgtcagcgat ccctactgtg ataccggcat gtgtcgaatg tttgtaccct aagtctttca
    28441 tacggtcaag catcttagaa gtttctgtta ctttgaatcg tttgaagact tctgcgatga
    28501 tgtttcctaa gtttttcttc ttgaacggta atactaattc ttgctctttg atatgtgcag
    28561 gaatatcagt accagcttca acaaagtatt tgtccggtgt ttggacggtc aagttgaagt
    28621 ctgttggttc attcagataa gggaattcag gaggcatgat ctcgttgaag atgatcttac
    28681 caactgttgt gatcatcgta cgttcttttt gccattcagt aaatggtttt tcacctaatg
    28741 agttagggtt cacaccgata cgtgaatgta aatgaacata tccgttgcgc catgcgataa
    28801 cggcttcgtt ttgatctctg aagaccattc cttcgccttc gcgtccttct tcttccattg
    28861 tcaagtagta gttacctaga accatatctt gggaaggagt aacaactggt ttaccatctt
    28921 tagggttcaa gatgttttga gcagctaaca tcaacatacg tgcttctgct tgtgcttctt
    28981 cattcaatgg aacgtggaca gccatttggt ctccatcgaa atcggcatta taagcttcac
    29041 atacaagtgg atgcaaacgg attgcgcgtc cttgtactaa aactggttcg aatgcttgga
    29101 taccaagtcg gtgaagcgta ggtgcccggt tcagcaatac tgggtgttcc ttgattacgt
    29161 cttctaaaac atcccatact tcgtcttctt gacgttcgat tttacgtttc gcatttttga
    29221 tgtttgaagc tagttcgcgt tgaacaagct cacgcatcac gaatggtttg aataattcaa
    29281 tggccatttc ttttggaagt ccacattggt acatcttcaa gaaaggtcca acaacgataa
    29341 cggaacgacc tgagtagtcg acacgtttac ctagcaagtt ttgacggaaa cgaccttgtt
    29401 tccctttcaa catatgagaa agagatttca atgggcggtt acctggtcca gtaactggac
    29461 gtccgcgacg accgttatcg atcaatgcat ctactgcttc ttgcagcata cgtttttcat
    29521 tttgaacgat gattccaggt gcattcaagt ctaacaaacg tttcaaacgg ttattacggt
    29581 tgattacacg acggtaaaga tcattcaagt cacttgttgc aaaacgtcca ccttctaatt
    29641 gaaccattgg gcgtaaatct ggtggaataa cagggataac gtccataacc atccagcttg
    29701 gttgatttcc tgaagcgcgg aatgcttcta aaatatccaa acggcggatt gcacgtgtac
    29761 gtttttgtcc ttgtgcagat ttcaattctt ctttcaattc ggctgcttct gcatctagat
    29821 caacattatc aagtaattgt ttaatcgctt ctgcacccat agcagcttgg aattcttgtc
    29881 catattgttc acgtttttcg cgatattcgc gttctgtcaa caattgtttt ttctctaaat
    29941 tcgtatttcc tggttcgatg actacatatg aagcaaaata aatgatttct tccaaagcgc
    30001 gtgggctcat gtctaataca agacccatac gtgaaggaat ccctttaaag taccagatat
    30061 gtgaaacagg agctgctaat tcaatatgtc ccatgcgttc acggcgaact ttcgaacgag
    30121 caacttccac tccgcatcga tcacaaacga ttcctttata acggatacgt ttgtattttc
    30181 cgcaggcaca ttcccagtct tttgtaggac caaaaatccg ctcacagaac aacccttcgc
    30241 gttcaggttt taaggtacga tagttaatcg tctcaggttt ttttacttca ccgtaagacc
    30301 agcttctgat tttttcggga gaagccaaac ctatttgcat actttcgaat ttatttacat
    30361 cgatcaaaag gggttccctc catttcattt aatggcaaac cgcaagacgg ctgatcttcg
    30421 accgccttgc agctcataga ccggattagt ctaaattatc ttctgcggtt tcaaagtttt
    30481 ggatgacatc gtctttttca tcttccactt gttcagcagc tttcttttct aattctttgg
    30541 cagtttgctg ttcggcaaat tttgtcaaag catcaacagt gatcaaatcg tcgtcttcat
    30601 cgtccatgtc gcgaagttcg atctctgttt cttcgatatc caagacacgc atgtccagac
    30661 ctagtgattg caattctttc accaatacgc ggaatgattc cggtacacct ggttttggaa
    30721 ttggttcacc cttgacgatt gcttcgtatg ttttcacacg tccgactacg tcatctgatt
    30781 tgtatgtcaa gatttcttgt aaagtataag ccgcaccgta agcttccagt gcccatactt
    30841 ccatttcacc aaaacgttgt ccaccaaatt gagctttacc tcccaatggt tgttgtgtaa
    30901 ccaatgagta aggtccgatt gaacgtgcat gcaatttgtc atcgaccatg tgagcaagtt
    30961 tgatcatgta catcacaccg acagacacac gtccatcaaa tggttcacct gtacgtccgt
    31021 cgtacagaac agttttcgca tcgctggcca taccggcttc acgaactgtt tcccatacgt
    31081 cttcatcact tgcaccatca aatactggcg ttgctacgtg gatacctaac tgacgtgcag
    31141 ccatacccaa gtgtaattcc aatacttgcc cgatgttcat acgtgaaggc acccctaaag
    31201 gattcagcat gatatcgatt ggtgttccat caggtaggaa tggcatatct tcttccggca
    31261 taatacggga tacaacccct ttatttccgt gacgaccggc cattttatcc ccttcatgga
    31321 ttttacgttt ttgaacgata taaacacgaa ctaacatgtt tacgcctggt gacaattcat
    31381 ctccagcttc acgagtaaag attttcacat cgtgaacgat accgccgccg ccatgtggta
    31441 cacgtaatga tgtatcgcgg acttcacgag ctttttcgcc aaagatcgca tgcaatagac
    31501 gttcttctgc agataattct gttacccctt ttggcgtgac tttccctaca agcaaatcgc
    31561 catcttggac ttctgcacca atacggatga tacccatttc gtctaaatct tttaatgcgt
    31621 cttccccgac attagggatt tcgcgtgtga tttcttcagg tcctaatttt gtatcgcggg
    31681 cttctgattc atattcttca atatggatag aagtgtatac gtcatctttt accaaacgac
    31741 ggctcatgat gatcgcatct tcatagttgt acccttccca agtcatgaaa ccaactaaaa
    31801 cgttttggcc tagagccatt tcgccttgtt ccatagatgg tccgtctgcc agagtatccc
    31861 ctttttcgac tttctcaccc aaatgaacga ttggacgttg gttgtagctt gttccggagt
    31921 ttgaacgacg gaattttgtt acatcgtatt tatctaatgc accgttgtcg cggcgtacgc
    31981 ggatttcaga agcatcaacg tattcaacga caccgtcatg tttacataac aaagcagctc
    32041 ctgagtcatg cgctgatttg tattccatac cagtaccaac ccatggtgct tgaggattga
    32101 tcaacggcac tgcttgacgc tgcatgttgg cacccatcaa ggcacggttg gagtcatcgt
    32161 tttccaagaa aggaatacat gctgtcgcaa cggcaactac ttgtttaggt gatacgtcca
    32221 tgtagtctac tttatcaata gatacttcta agttttcact ttgagcacga gccataacaa
    32281 cgtcttgagc aaatgttcca tcttcattca atgggctgtt tgcttgggct acgatgtaat
    32341 ggtcttcgat atcagctgtc aagtagtcga tttggtcagt aacacgacct gtttcacggt
    32401 ctacacgacg ataaggtgtt tcgataaatc cgaatttatt tactttcgca taactggaca
    32461 aactattgat caacccgatg tttgggcctt caggagtttc gatcggacac atacggccat
    32521 agtgggagta gtgaacgtca cgaacttcat aaccggcgcg gtcacgagtc aatccgccag
    32581 gtcctaaggc tgaaagacga cgtttgtgtg tcaattcgcc aagagggttt gtttgatcca
    32641 tgaactgtga caactgagaa gaaccaaaga attctttgat acttgccaca actggacgga
    32701 tattgatcaa ttgttgaggt gtcaatgttt cagtatcttg gatcgacatt ctttcacgaa
    32761 ccacacgttc catacgtgct agcccgatac ggaattggtt ttgtaaaagt tcaccgactg
    32821 aacggatacg acgatttcct aagtggtcga tatcatcgac attgccgata ccttccatca
    32881 agttcaagaa atagctcatt gaagcaatga tgtcggctgg acgaaccgtt tttacagctg
    32941 cttctggata gccgtttccg atcacgttta cttcacgttc agggtctctt ggtgaaaata
    33001 ctttgatgac ttggacagtc attggatctg tgaccacacc gtcttctgat ggatagtaag
    33061 tcactgaatt taggccatta tcaatgaaag gagctagtgt ttccatgact tggtgagtca
    33121 gaactgttcc tttttcaacg atgatctcgc ctgtttctgg atcaactaat gtttcagcta
    33181 atgtcagatt caatagacgt gttttcaaat ctaatttttt gtttactttg taacgaccaa
    33241 cgtttgctaa gtcgtaacgt tttggatcga agaaacgagc attcagcaag ttacgtgagc
    33301 tgtctgctgt ttttggttcg cctggacgca aacgttcgta aacgtctttc aaaccttctt
    33361 ctgttcttga gtcgcttgcg tttttatgca agtctttttc gactgtattg cgtaatgttt
    33421 cgctgtcgcc gaagatttca aagatcgtat catcggaacc aaatcctaac gcacgaacta
    33481 atacggttaa aggaatctta cgtgtacggt cgatacgtac ataagagatg tcttttgcat
    33541 cagtttccat ttctagccaa gctccacggt tagggataac agtagaaccg aatccttctt
    33601 ttccgttttt gtcgactttt ccatggaaat agacacctgg tgaacggact aactgagaaa
    33661 cgattactcg ctctgccccg ttgatgatga atgttccttg ttctgtcatt aatgggaaat
    33721 caccgaagaa tacttcttgt gctttgattt cgccagtctc gcggtttgtc aaacgcaatg
    33781 ttacatgtaa tggtgcagag tagtttgcat catgcgcacg agcttctgct actgtatact
    33841 ttggttcttt caattcataa tctacaaatt ccaaagatag attgccattg aagtcatcga
    33901 ttggcaaaat gtcttcaaac atttctctaa gtccctcatc taagaaccat tggtaagagt
    33961 ctgtttgaat ttcgatcaaa tttggtaatt ccaacacttc actgattctt gcgaaacttc
    34021 tacgttcgcg atgttttccg tattttacta cgtgtccagc caagctcttc acccctctaa
    34081 aatgtcattc aaaaagacta cccgatcaat tgttacaata atatttcaaa tattattttt
    34141 aggtaacaac agacattttt tgcggctttt aggcaaaaaa aaaccaaaaa tagaacattc
    34201 tcttgaaacc ttacttcgtg agcttctact tttgtttcct tatattaaag ccgaaacgga
    34261 caatatttcg tttctgcctt ttcttcatca gatagttttt gcaacttcat atactacact
    34321 tattttctat aaaagtcaac acttcctggc gtttcttctg ctttttcgca acattttctt
    34381 aatgttttca attttatgtt ttcaattatc tgacaattct taattgacag acctttttat
    34441 ctcggttata ctttagaaaa ttcattttgt ggaggaactt ctatgcgaac caacatcaac
    34501 caaatcgcac caacacaact gaataacttt tactttagct actttagata ggtttgtatt
    34561 catcatgatg gatacaaacg ttaaacgaca gtttgtatct atgatggcta aagaactttg
    34621 tattgcagca aaaagccacg tagacaaaat gctaagtggc tgagcagtga aaatacaaag
    34681 taaaagtatc aagcattcta ctttttcaat ttattttccg acttttcaat ccatttggac
    34741 attttgtctg aatggatttt ttgtttccca aaattaagga ggaaaaagaa atgaaaaaaa
    34801 atataatcat cggattaggc gctgccctgc tactagcttt aggaggttgc gcacaaaaag
    34861 acaattccgc agaggcatca gatggacaaa aaacaattgg cgtattgcaa cttgtacaac
    34921 ataactcgtt agatgctgcc tataaaggat tcaaagaagg attagcagaa gaagggtata
    34981 aagaaggcga taatctaaca atcgattatc aaaatgccca aaacaatcaa gacaacctga
    35041 aaagtatgag tgagaaactc gtcaaagaag atcctgattt gctacttgga atcgccactc
    35101 cagcagcaca atctttatta aatgaaacaa ccactatccc gatgactgtc acagcggtca
    35161 cagacttgga acaagcccat ttagtcacat ccaatgaaaa acctggcggc aatgtgactg
    35221 gcacgacaga tatggtgcca atcgaaaaac aaatcggctt actgttaaat atcgtgccag
    35281 acgccaaaac aatcggcatc atgtataaca acggtgaagc aaactctaag atccaagctg
    35341 atttggcaga aaaagaattg aaaaaagcgg gcgtcaaagt caaagtactg acagcaaatt
    35401 ccacaaatga tgtccaacaa gtgacgaaaa gtctagctaa agatgtagat ggaatctata
    35461 ttcctactga taacacgttc gcaagtgcgg caagtgttgt cggtgaagtc gcaaaagaaa
    35521 caaaaacacc tgtcgttgct ggcgcagttg atcaagtcaa agacggcggt ctagcaacag
    35581 taggaatcga ctacgaaagc ttgggtaaac aaacagggat catggcagca aaaatcttag
    35641 acggcaagaa tccaagtgaa cttcctgtcg aaacagcgaa tgatttggaa ctgtatgtca
    35701 ataaagacat ggccaaagca ttaggcatca atccagacag catcaaagaa gctgaataaa
    35761 acacaggagg attctcatga caaatttgct ctctcttttc ttatcatctg catcgcaagg
    35821 ggtcttatgg gccctacttg cgattggtgt atacgtcact ttccgtatct tggacattgc
    35881 cgacttgact gctgaaggaa gctttccttt aggtgcaggg atcgcggcag taagtattac
    35941 aaatggctat catccattga cagcttgtct tctaggcttt ttagggggtg cggcagcagg
    36001 gcttgtttcc ggactgcttc acacaaaatt gaaaattccc gcccttcttg cagggatcat
    36061 caccatgact ggtttatatt ctgtcacatc ccgtgtgatg ggtgcgccga acatctcttt
    36121 attgggtcag ccaacgatct ttcattgggt cgagtcattc ggcataacaa aagacaacgc
    36181 tgtactgatc gtcggtctgc tttttgcttt gatcgttgta tgtttacttg ttttgttctt
    36241 acgtacagaa acaggacttg ctattcgtgc aactggcgat aatctggcaa tgagcgaagc
    36301 aaacgggatc aacacagata atatgaagat catcggttat atgatttcta atggatgcat
    36361 tgctctttct ggctccttgc ttgcgcaaaa caacggcttt gctgatttaa attctggtat
    36421 tggaacgatc gtaattggac ttgcttctat tatcatcgca gaagttttgt tacgtaacca
    36481 gccactattt ctccgtctct tgactgtcgt tttaggtgcg gtcatttatc gttttatttt
    36541 agcgttagtc ttcgaactga atgttgaacc aagtgattcg aaacttgctt ctgctttcgt
    36601 gctagtgatc tgtctttctt tcccgcagat ccagcaaaaa ttccatttac ctcaaaaaaa
    36661 gaaaggcggt agtgtcaaat gaatgacgtc ttaacattgg aaaatctcca tcataccttt
    36721 gaaaaaggaa cgatcaacga aaatcatgtc ctgaaaggca ttaatctttc cttagatccc
    36781 ggcgacttcg tgacgatcat cggtggaaat ggggctggta aatctacttt gctgaacagc
    36841 atcgccggta cattttctgt cgatcagggt caaattttat tgaatggaaa aaatatcacg
    36901 aaaaaatctg ttgtcgaacg ttcgaaatcc atcagtcgcg tattccaaga tcctaaacta
    36961 gggacagccg ttcgtttaac ggtagaagaa aatcttgctc ttgctatgaa acgaggaaaa
    37021 aaacgcggct tcttccgtgg tgtcaaacca caagaccgaa catttttcaa agaccagctg
    37081 gttcggctaa atttggggtt ggaaaatcgt ttatcctcag aaatcggttt gctgtctgga
    37141 ggacaacgtc aagctattac gttattgatg gccacattac agcgtccaga actgatctta
    37201 ttagatgaac acactgcagc gcttgaccct gctacttcca aaacggtgat ggaattgaca
    37261 gaaaagctga tcactgagca gaatctcact gcttttatgg tcactcatga catggaagac
    37321 gcgatccgct atgggagtcg actgatcatg ctgcatcaag gaaggatcgt cgtagatatc
    37381 cagcaagagg aaaaacagca gctcacagtg ccccaattat tagcgttgtt caaacaaaac
    37441 agcggcacgg cattaaaaga cgatcaagta cttttatctt cataatcaga aagaggatgg
    37501 gacaaaaaat gaacgcccac cctcttcttt tttctctttc cttctttcgt ttttgcttta
    37561 tgtatcaacc gttgtttctt cgaaaactat tttgttctct ccttcttcct gatagaatgg
    37621 atgggaaagg agcttgatgc catgacaacg aaagacaagg tacttcaatt attgaaaaag
    37681 acaccagatt ttctttccgg agaaaagttg gcacagcaac tggaagtttc ccggacaagt
    37741 atttggaaag ccatcaaaga attggaaaag gcaggctatc gttttgagca cgggcctaca
    37801 gggtatcgtt acctgccctc tgacgtacta gatgcaaaag aaatccagca agagctacgg
    37861 tcgtttattc ctgatttgaa agtacaaacg atgttgactt ctgaatctac gatgaaagat
    37921 gtcaaactgg cagcagctca aggagattct gtttcgaagt tatttgttgc tgatacacaa
    37981 gaacaagctc gtggacgttt tggacggcct tttttcgctg aacctggacg aggaatctac
    38041 atgagtctct tgctccatcc agacaaacgg tttgatgaat tgcctcaata tacgatcatc
    38101 gcagccagtg cctgtgtgaa agccattgaa tctgtaaccg gtcgttctgc ttcgatcaaa
    38161 tgggtcaatg atatctacat ggatggaaaa aaagtctgtg ggattttatc tgaagcagtc
    38221 agtgatatgg aaagcggcag gatcagtcat atcatcattg ggatcgggct taatttttct
    38281 gttcctcaaa atcattttcc agctgatctc caagaaaaag ccatctctgt ctttcccgac
    38341 ggacaagcta gtgtcacaag gaatcagcta atcagcaaaa tctggcaaga attgttcact
    38401 tatctttctc acccagaaaa agcgttggac ttctataaag aaaaatcatt cgtattggga
    38461 aaaaccgtgt cgttcaatca aaaaggaacg acttatgtcg gtcttgcaaa agaaatcggt
    38521 gatcatggcg aactgatcgt ccaaacaaat gaacagaaga tgagtttgtc ttctggcgaa
    38581 atcagtctca catctatccg tacgcaataa ttatcagatt tcacaaaagg acacctgatc
    38641 tcatacagtg tccttttttc tgaattctct tcaaaagact ctccttattc tatcagctga
    38701 cagtttctct atcctctaga aaaaggtatt ctaaaaatat ctataaagca tggtacactt
    38761 tctttaaatg aatcaatgca aaaaacaaga gagtcatcag tttacacgat caaaagagat
    38821 agcaggccgc ttcctttttc aatcttatag atagaccatg ggtaacgata aaaacaaaaa
    38881 agaacttgcg acttattttg ccgtaagttc ttttttagct agtgccttaa tctcttaatc
    38941 ccaacaaacg tctcgcatag atcacctgac cagagtgcat agcagcatca tcaataatag
    39001 aaacgagacg cattcctcgt gtgactggtg gtgtccagtt ctcgtcaatt acttcatcta
    39061 gctcggaagg ttccagcctt cttaagtaag aaatagcaaa atcagcagct cctcgcaagt
    39121 atcctagcag atcttctatt tgattagctt ggatctgctt tgcttgttcc agactatgct
    39181 tccaatcttg ttcgttatct gctatcgtca tcggaaaccg gctttcccag tcttgactat
    39241 gccaaattgg agaagttcct gctaaatcag caatctggaa atctaattct cgagcggtat
    39301 gccaagccag ccaagtcatt gatttgatct gcggggctct atcttccact ggaaaacgat
    39361 tggcctcctc aatagagacg ccttcaaaca aacgttcaaa ccgatcgtat gcccgatcca
    39421 cactctcgca tagcatatct aaataatcca tttattttcc tcctttttat ttatcctact
    39481 tactttttcc taattctagg atacctactt atatgttact acacaccctt attttttaca
    39541 ctttaacagt caccatcatc aatagtttgg agattgaaag aaaaagatag tgtcgaacta
    39601 tcaattgaac aagaagcaac aatatgaacg tttcattaat attctttaaa taaacaatga
    39661 tttttcaaac gaacagttta agactaggag aaaaagagta taataggaaa atgtttgaaa
    39721 ggaaaggaag gggaaaaatg aaaaaaaggg caagtttact agtcctagtt gctttgttgt
    39781 tcaatatctt ttcgtatggt gtggccgttt cagcggaaac atacattcct aaagaccctg
    39841 ggaatattta taatgatcta actggtaact atggcttgtt aggaattgct agtcaattcc
    39901 acgtatttgc taaaaataag acaactgtga atgcacatac agacggaaat atagcaacac
    39961 atgaacttga tgcaaaaaat aattttggta cagagcttca tgaaggttta ttgaaccaag
    40021 aaataaatta tatacaaaaa actgattctt tcgctaattc ttcaggcata cctacaggta
    40081 gcacaagaat gaataaattt gtcgttggca gtaacgtgga tgtagcactt caagatggtc
    40141 atcctacagt caatggatcc aggcttgatc acttaactcc agatgatttt tataaagatc
    40201 gtgctgggca tgaatatatt gatttcgagc aggaatttgc taaattgaat gtagcagcaa
    40261 atgatcttgc aacgatcact ccagctaaaa catatactgc tgcagatttt ccagacatga
    40321 acaatcggac aatcgattta actgatttat cagataccgg gtttttattg gtaaatatcg
    40381 atgcagaagt gttaacgatg aatacgccac ttcaaatcat taatccaaat gatcaagtag
    40441 ttgtatttaa tgtcatcaac agttcttctg cattgaacgt acagtcacca atcaaataca
    40501 atgatcgttc aaatcatgaa acagaagatt tttcggatgc gaatatctct tggaattttg
    40561 gaaatgagat gactgatttg acaatcagtg ctccttttca aggaacaatc cttgcgccta
    40621 atgctactat ccgcattact caaaaccaag acggaacgat tataggaaca aatgtgattt
    40681 tagatgcagc taccaatcgt tgggatccta acgaaatatt tatcagcgac aatggtacag
    40741 atacgacaga cacaagcgac agtacagata catctaccac aagtgatagc tcagattcat
    40801 caacttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg gatacatcag
    40861 cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggac acatcagctt
    40921 caagtgacag tacagatact acctctacca caagtgacag cacggacacg tcagcttcaa
    40981 gcgacagtac agatactacc tctaccacaa gtgatagcac ggacacatca gcttcaagtg
    41041 acagtacaga tactacctct accacaagtg atagcacgga cacgtcagct tcaagcgaca
    41101 gtacggatac atctaccaca acaagtgata gcacggacac gtcagcttca agtgacagta
    41161 cagatactac ctctaccaca agtgatagct cagattcatc taccactaca agtgatagca
    41221 cggacacgtc agcttcaagt gacagtacag atacatcctc taccacaagt gatagctcag
    41281 attcatcaac ttcaagcgat agtacagaca cgtcgtctac cacaagcgat acacgtcatg
    41341 caagtgtctc cttctcaaaa acaagttcgg ataacaggac gaactttaac ttaactaata
    41401 aaacaagcaa tgacaatgga aacaaatcaa aaagagcact tcctaaaact ggaagtcaat
    41461 cgaataattg gataacactc gcaggcgtta tattacttgt aatcggttta agaatcactt
    41521 ttatgagtta taagaaatcg aaaaggtaaa aaaatattca agataaaaaa gagcaacgtc
    41581 gctaacggac gttgctcttt atttttgctt tttatcataa atacaagaac tacaattcaa
    41641 tttgtcaaaa aacacgtaga tttccttctg agaaatttat ttacgtatcg aacctttccc
    41701 tattcagtct ttgcgatcca aaatcagttg ggtaagggaa aagagattct cttttgttcg
    41761 aagagaggtg gcaggtaatt tctttatttc cttcaaagcc ttttctgtgt aagaggccgc
    41821 taacttccta gcttcttcca cgcctcctgt atgttcaacg attttgcgta cggcgtcgac
    41881 atcttcttct gtcattgccg ctttcttctc taaataaggt agtagctttg gtttgttttt
    41941 ttgcaaagag tagatcaacg gtaaagaata gaccccttga cgcatgtctt ctagtacagg
    42001 tttgccgatt tcctggctct cttttgtata atctaaaata tcatcgataa tctgaaaggc
    42061 catcccgata gaaagtccga tatctcctgc tttttttgcg aagcgctcgc ttgttccact
    42121 ttcaaaagcg ccaacagaac aacttaattg gaacagttca gctgtcttac ctgaaatatt
    42181 ttccaaatat tcttttacag aaagttcata attatagcga tcatccatct gccctaattc
    42241 gccattcaat actttttcca tactgcgaga attcaattgg agacttttca aggaagtgga
    42301 gtaatcagac aatagtttaa aacagcagac aaataaatag tctcctgcat agacagaaac
    42361 gacattacca aactgactac ggatattagg ctgaccacgt cgcagatccg cttcatcaac
    42421 gatatcatca tggatcagtg tggccgtatg gagcatttcg atagaagctg ccaaagcgat
    42481 ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac aatagctgat aggcaggacg
    42541 caacagcttt ccgccggcat ggatcgtttc tttgattgct tgttcgacag gtttgttttt
    42601 caggcggaca gtttcttcca tccgttttag tgtttctgtc aattctttac ttaaagcagg
    42661 atagttttcc cacattggat gtagcttcat aaaaaaactc cttgtgtgac gattattttt
    42721 tggtaaacat tgggctccat ttttgtttgg ttccctgctc ataatctttt tggaatcgtt
    42781 gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat cccgattgca atccctgata
    42841 aaatccctaa aactgaaagg actggcaagt acaacatgac tgtccatgat tgtgcaatga
    42901 aagaagccat caaaagctgt ccgacattat gcatgaaacc gccggccgca ctaattccga
    42961 tgatactgac acgttttggt cctaattgct tgaccaataa cattccaaaa taactaagca
    43021 aagcaccact catactataa aggaatgtgg aaatcgttcc gcctagcaaa gtagtcagga
    43081 atagtcgtag ccaaagtagt aagaagctgt ctcgtttttt catggtaaaa atggcaacga
    43141 tcgtgattag attggctaag cctagttttg caccaggcgc aaaagaaaaa ggataaggga
    43201 tcatattttc caataaacca atgatcactc cttgtgcaac aagtagcgaa atataaatca
    43261 ttttttgtaa tttactcatg gtgtgtgaag gtctaataaa tcagactgtc ttcgtcacct
    43321 ccatctgttg aacggacttc gatcactaat ttatgtggca aacagacgat ggtttcgcca
    43381 tttttcgttg cccagcctcg acgtacgcag atctggtcat cgcaatctgc ttcttttatc
    43441 cgtactcgat cgccatctat ctcgatcaag ttataatctc cgtgttcatc tttgtaagta
    43501 tatgtgttgc tctcttctcc agccatcaaa gggaatgttt tgatttctgt gccatccaca
    43561 cgcagtaccg cttctttttc tgctgttgcg gtgttttgct gaagaccaaa aatcatgact
    43621 ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa catcccaagg tttgagcaga
    43681 cttttttcag caaattcttt aaggctcatt tttgttctcc tttttccaga ttaactcacc
    43741 caattttagc ataaaaacaa gaggttgtga cacctgtgtc ttgctttgag taataagaac
    43801 gaagaataga aaacagcttt ccatgttttc taattctttg ggcttattac cgagaagcag
    43861 caggtggaac accgtttata acaagaggtt gtgacagaag tgggcaactt caagaaataa
    43921 gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa tttcggctta tttttcgaag
    43981 aagctacttc tggaacaccg tttataacaa gaggttgtaa cagaggttgt gacacctgtg
    44041 tcttgctcat ggaggcttat ctcgggcatc ttattccttt aattttacat aaaaaggagg
    44101 ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg cattcaaata acgttcttta
    44161 tctatttatt tcttgccgta ctgggaactt ggtctaccgt accaccaatt acctagtgta
    44221 cgttgtagcc atggaattac ccagcgatcc aacccaatcg cacgtccgga accgttcatc
    44281 aatgcgaaag caacaaaaat gaaccaaatg tttacccagt aaaacatgcc tgataagcag
    44341 aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc atgtgaataa tccagcaatc
    44401 aatgctaatg ccaaggcaat ttcaaagaat accatgaatt tttggaagaa cattgctaca
    44461 tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc agtctggtga cttctcaatc
    44521 acagccattg gttcttcacc gtatgcataa ctcaaaccaa aatgtgcagc ttgagtcgct
    44581 gcttctccgg cacttgcccc tgctccgctg gcagctgtgg ttgcatcacc agcagcagaa
    44641 gcaccactgg caacatctgt tacattgtat tcccatgtcc aagggaacac actgtttcca
    44701 gtgaaccatg atccttcacc aaaccaagta cttggtttga acacttcacc attcccaatg
    44761 atttttttgg aagcttcaac gaaccaaact aaaccataga aaatacgaag tggcacactc
    44821 cataagacat tgccgtaacg agaagaatgt cctcgagtca cgttgcgatc atctttgata
    44881 tggaaaaact catgcattaa atattggaac atataataac cagaacgaat atcaaagaag
    44941 tatttcaaat tcacgatatg tttcattacg atagctaaaa atccgcttaa atggatctta
    45001 tcaaataggt tagctacccc ccatttggca ccaatagaaa ccataaatcc ttgatagttt
    45061 cctttaaatt tatgcttttc tccgcctttg atcgaagcca cgatattcgc tgcggctgtg
    45121 tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg gtgttcctgg gaattcttca
    45181 taaaagacta aatcaccaat gatatagatg tttttatctt cgtagccttt tgcctgcata
    45241 tattcattgg cgatcaaccg ctgtccgcgt gctgattcta atccaaagtc agcagcgtca
    45301 gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag tcggaatgtt gcgtccgtct
    45361 ttcaatttga tatgatcagc agccacttct acgattggtg cattcaataa taattccacg
    45421 ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat ttcgatccaa catattcaag
    45481 atcgtcggca ttgcctctac gaccatcagt gtgatttcgt ttggatctaa tttataatct
    45541 ttggctaggc gatttttcca gtcgatcagt tccccaatca tttcaatacc ggtaaatcca
    45601 gaaccacaga caacgaaagt cagcatggct ttacgcagtt catcatctgg ttccaatgca
    45661 gcttttgcta ctgtcttctc aatatgttcc cgaatcctta cagaatcttc aaatgaccaa
    45721 agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt cattcggctc tccgcccatt
    45781 ccaaggatca gctgatcaaa aggataggaa cctaatttgg tcttgacgat ttttcgttct
    45841 ttatcgatac cgattacagt gtctgtgaca agttgtacat tcttttgacg agcaaacaaa
    45901 tgctgcagat cgtattggat tgcttctggt tctacacgtc cgccagctac ttcatgcaac
    45961 tcggtcatca tagtatggta cgaatgacga tcgatcagag taattgctac atcttctttt
    46021 ttcagtttct ttgccaagta ttttgtagct gaaacaccgg catatcctgc tcctacaacg
    46081 acaatatttt gctttgccat tgatcatccg ctccttatag actatctata taatgaatat
    46141 atgataattc ttttagtttg atacttatta cacaatctcc atatattata tagatgttct
    46201 gttcttttgt ctaactcctt ttggattttt ttcaaatttt aaaataaagc gttttttcac
    46261 gaaatttgct gaaagaaaac gtttgaaata ttacatgggt tatttatcaa aaatagttga
    46321 cttataggct aaaaagatta gaatgtataa tgaatcacaa agaaaaattg tggactagaa
    46381 atggaatggt ggggtattaa aatgaagaaa aaacgttttg taactggatt tgccgtatta
    46441 gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg acaacggaag ctcaaactct
    46501 tcttctgaat catctacagc acaatcttcg actgcaaaaa gcacaacaga atcatctgca
    46561 aaagttgtag ctggcggaga tttgcaagac ggcacatata aattagaaga aaaaaattat
    46621 tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag acggcaaaat cactgaatca
    46681 aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa aagatgcaga atacaacaaa
    46741 aacatggaag caaaatctgg cacaaaccca gaaaaataca ttcctgagtt gaatgaccaa
    46801 ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa ctggagctac tcactcatct
    46861 gaaagcttcc aaaactacgc acaacaattg atccaagctg ctcaagctgg taacacagat
    46921 acaatcgaaa tcgacaatgg tgctgatttg aaagacggca cttacaagct tgaagaaaag
    46981 aactactcta acggctaccg tgtgcaattc gaaatgacag ttgctggcgg caaagtaact
    47041 gaatcaaact ttgattatat cgacaaagac ggtaaatcaa aacaagatga tacagaatat
    47101 aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa cttacatccc tactctgaac
    47161 gacgaattcg tcaaagcaat gggcgaagaa gatggctcac ctgctaatgt agaagttgtt
    47221 acaggtgcta cacacagctc acattcattc atcatgtacg cacaacaatt agtgaatgca
    47281 gctgaaaaag gcgacactca aacaatcgaa gtagataata tcgtaacaga aaaataatca
    47341 cataatcata ctaataaaag ttggagggtg ggacgatagc cagattaggc agtcgtctca
    47401 cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact actattttcc ttaatcacac
    47461 tgggcatctc ttttatggtg ctaactggct gcagcaactc agcaaataaa acaaccactg
    47521 aaagttcagc agctaccaag atattgaaag atccatattc tgatgaacag ttcttgttag
    47581 ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga atcagcattg aaacctgcct
    47641 tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa tcaaaaaggt tctgagatcg
    47701 atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt ttctgatgac gtctacacgt
    47761 tagtcaagcg ggcctacgaa tatagccaag attctcaagg cggattcgat atggcgattg
    47821 gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc cagaaaacct tcccaagaag
    47881 aaatcgatca ggcattgaaa ttagtggatt atcataagat cgaactaaat gacaaagaaa
    47941 aaactgttta tctaaaagaa aaaggaatga tcatcgattt aggtgcaatc gccaaaggtt
    48001 acattacgga cgaagtagtg aaagtcttga aaaaacaagg agtgaccact gccatcgttg
    48061 acttgggcgg taatgtctat gtcttaggcc acagcccaag aggcgaaaac caagactgga
    48121 cagtcggcat ccaagatccg aacatggctc gcggatctgt tttaggttct attaaagaac
    48181 ggaacaaaac attagttacc tctggaattt acgagcgtta tttagaagta gatggcaaaa
    48241 aataccatca tttgtttgat ccaaaaacgg gttatccgtt cgacaatgat atcgcaagtg
    48301 tcacgatcat aacagataaa tcaattgatg gtgacgggtt gtctacagca gtcttttcaa
    48361 tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt gaataacggg acggaagcga
    48421 tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc aatcaaagac accttcaaat
    48481 tatcggagga ctcccattac acgatgggtg accgttctga actaaaataa ggagtacgaa
    48541 ccatgacact aaaaatcttt ttagaagtag ttgaaatacg aacaaaggtg gctagtgtat
    48601 ttccttttgc ggtaggggta ttattctcta tcgctttttt tcatgagata cattggctaa
    48661 acactttttt attttttatt ggaatgctag tttttgattt agcgactacc gctatcaata
    48721 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
    48781 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
    48841 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
    48901 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
    48961 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
    49021 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
    49081 ttgctttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
    49141 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
    49201 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
    49261 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
    49321 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
    49381 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
    49441 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
    49501 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
    49561 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    49621 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
    49681 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    49741 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
    49801 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
    49861 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
    49921 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
    49981 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
    50041 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
    50101 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
    50161 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
    50221 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
    50281 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
    50341 aaaaaacaaa aaaccagaaa atcagtatac tgat
//
LOCUS       DACCWK010000009        50268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCWK010000009 DACCWK010000000
VERSION     DACCWK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.009"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            51..2648
                     /gene="adhE"
                     /locus_tag="IVW65_000459"
                     /gene_synonym="adhC"
     CDS             51..2648
                     /gene="adhE"
                     /locus_tag="IVW65_000459"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7899385.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2877..3983
                     /locus_tag="IVW65_000460"
     CDS             2877..3983
                     /locus_tag="IVW65_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7899386.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4000..5028
                     /locus_tag="IVW65_000461"
     CDS             4000..5028
                     /locus_tag="IVW65_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP7899387.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5034..5513
                     /locus_tag="IVW65_000462"
     CDS             5034..5513
                     /locus_tag="IVW65_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7899388.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5529..6824
                     /locus_tag="IVW65_000463"
     CDS             5529..6824
                     /locus_tag="IVW65_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP7899389.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6835..7659
                     /locus_tag="IVW65_000464"
     CDS             6835..7659
                     /locus_tag="IVW65_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7899390.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7824..8723
                     /locus_tag="IVW65_000465"
     CDS             7824..8723
                     /locus_tag="IVW65_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7899391.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8849..9718)
                     /locus_tag="IVW65_000466"
     CDS             complement(8849..9718)
                     /locus_tag="IVW65_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7899392.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9871..11331
                     /gene="rhaB"
                     /locus_tag="IVW65_000467"
     CDS             9871..11331
                     /gene="rhaB"
                     /locus_tag="IVW65_000467"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP7899393.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11328..12584
                     /gene="rhaA"
                     /locus_tag="IVW65_000468"
     CDS             11328..12584
                     /gene="rhaA"
                     /locus_tag="IVW65_000468"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP7899394.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12598..13443
                     /gene="rhaD"
                     /locus_tag="IVW65_000469"
     CDS             12598..13443
                     /gene="rhaD"
                     /locus_tag="IVW65_000469"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP7899395.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13445..13756
                     /gene="rhaM"
                     /locus_tag="IVW65_000470"
     CDS             13445..13756
                     /gene="rhaM"
                     /locus_tag="IVW65_000470"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP7899396.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13838..14989
                     /gene="fucO"
                     /locus_tag="IVW65_000471"
     CDS             13838..14989
                     /gene="fucO"
                     /locus_tag="IVW65_000471"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP7899397.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15116..15874
                     /locus_tag="IVW65_000472"
     CDS             15116..15874
                     /locus_tag="IVW65_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP7899398.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15974..16618
                     /locus_tag="IVW65_000473"
     CDS             15974..16618
                     /locus_tag="IVW65_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7899399.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16625..17632
                     /locus_tag="IVW65_000474"
     CDS             16625..17632
                     /locus_tag="IVW65_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP7899400.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17648..18490
                     /gene="kduI"
                     /locus_tag="IVW65_000475"
     CDS             17648..18490
                     /gene="kduI"
                     /locus_tag="IVW65_000475"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP7899401.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18510..19313
                     /locus_tag="IVW65_000476"
     CDS             18510..19313
                     /locus_tag="IVW65_000476"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7899402.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19562..20626)
                     /locus_tag="IVW65_000477"
     CDS             complement(19562..20626)
                     /locus_tag="IVW65_000477"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7899403.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20623..21708)
                     /locus_tag="IVW65_000478"
     CDS             complement(20623..21708)
                     /locus_tag="IVW65_000478"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7899404.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21721..22698)
                     /gene="mvaD"
                     /locus_tag="IVW65_000479"
     CDS             complement(21721..22698)
                     /gene="mvaD"
                     /locus_tag="IVW65_000479"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7899405.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNFKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22691..23635)
                     /gene="mvk"
                     /locus_tag="IVW65_000480"
     CDS             complement(22691..23635)
                     /gene="mvk"
                     /locus_tag="IVW65_000480"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7899406.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23957..24610
                     /locus_tag="IVW65_000481"
     CDS             23957..24610
                     /locus_tag="IVW65_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7899407.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24695..25600
                     /locus_tag="IVW65_000482"
     CDS             24695..25600
                     /locus_tag="IVW65_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7899408.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25602..26234)
                     /locus_tag="IVW65_000483"
     CDS             complement(25602..26234)
                     /locus_tag="IVW65_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7899409.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26554..27078
                     /gene="infC"
                     /locus_tag="IVW65_000484"
     CDS             26554..27078
                     /gene="infC"
                     /locus_tag="IVW65_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7899410.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27150..27350
                     /gene="rpmI"
                     /locus_tag="IVW65_000485"
     CDS             27150..27350
                     /gene="rpmI"
                     /locus_tag="IVW65_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7899411.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27403..27762
                     /gene="rplT"
                     /locus_tag="IVW65_000486"
     CDS             27403..27762
                     /gene="rplT"
                     /locus_tag="IVW65_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7899412.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28014..29351
                     /locus_tag="IVW65_000487"
     CDS             28014..29351
                     /locus_tag="IVW65_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7899413.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29399..31528
                     /locus_tag="IVW65_000488"
     CDS             29399..31528
                     /locus_tag="IVW65_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7899414.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31503..32192
                     /locus_tag="IVW65_000489"
     CDS             31503..32192
                     /locus_tag="IVW65_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899415.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32600..33286)
                     /locus_tag="IVW65_000490"
     CDS             complement(32600..33286)
                     /locus_tag="IVW65_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7899416.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQSYDQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(33422..33616)
                     /locus_tag="IVW65_000491"
     CDS             complement(33422..33616)
                     /locus_tag="IVW65_000491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899417.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33666..34106)
                     /locus_tag="IVW65_000492"
     CDS             complement(33666..34106)
                     /locus_tag="IVW65_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7899418.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34110..34955)
                     /locus_tag="IVW65_000493"
     CDS             complement(34110..34955)
                     /locus_tag="IVW65_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7899419.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35104..36522
                     /gene="pepV"
                     /locus_tag="IVW65_000494"
     CDS             35104..36522
                     /gene="pepV"
                     /locus_tag="IVW65_000494"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7899420.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36598..37800)
                     /locus_tag="IVW65_000495"
     CDS             complement(36598..37800)
                     /locus_tag="IVW65_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899421.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(37787..39574)
                     /locus_tag="IVW65_000496"
     CDS             complement(37787..39574)
                     /locus_tag="IVW65_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899422.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(39587..40540)
                     /locus_tag="IVW65_000497"
     CDS             complement(39587..40540)
                     /locus_tag="IVW65_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7899423.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(40725..41075)
                     /locus_tag="IVW65_000498"
     CDS             complement(40725..41075)
                     /locus_tag="IVW65_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7899424.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            41275..42354
                     /gene="pepA"
                     /locus_tag="IVW65_000499"
     CDS             41275..42354
                     /gene="pepA"
                     /locus_tag="IVW65_000499"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7899425.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            42497..42817
                     /locus_tag="IVW65_000500"
     CDS             42497..42817
                     /locus_tag="IVW65_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7899426.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            42839..43303
                     /locus_tag="IVW65_000501"
     CDS             42839..43303
                     /locus_tag="IVW65_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7899427.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            43499..44104
                     /locus_tag="IVW65_000502"
     CDS             43499..44104
                     /locus_tag="IVW65_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7899428.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(44143..45432)
                     /locus_tag="IVW65_000503"
     CDS             complement(44143..45432)
                     /locus_tag="IVW65_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7899429.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            45860..46339
                     /gene="rlmH"
                     /locus_tag="IVW65_000504"
     CDS             45860..46339
                     /gene="rlmH"
                     /locus_tag="IVW65_000504"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7899430.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            46692..47006
                     /locus_tag="IVW65_000505"
     CDS             46692..47006
                     /locus_tag="IVW65_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7899431.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            47022..47408
                     /locus_tag="IVW65_000506"
     CDS             47022..47408
                     /locus_tag="IVW65_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7899432.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            47437..48822
                     /locus_tag="IVW65_000507"
     CDS             47437..48822
                     /locus_tag="IVW65_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7899433.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            48825..48978
                     /locus_tag="IVW65_000508"
                     /pseudo
     CDS             48825..48978
                     /locus_tag="IVW65_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            49001..50206
                     /locus_tag="IVW65_000509"
     CDS             49001..50206
                     /locus_tag="IVW65_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7899434.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT        16094 a         9169 c        10658 g        14347 t
ORIGIN      
        1 atacatgtga attatttaac aaataaccat gacccattag gaggataatg atggctaaag
       61 taatagaaaa agaaaaaacg aagacaattg acgtacaagc aatgattgat gatttggcag
      121 aaaaagcaaa tgcagcattg aaagaaatgg aaagctttga tcaagaaaaa gtagaccata
      181 tcgtgcatga aatggcaatg gcagctttgg atcaacatat gccattagcg aagatggctg
      241 tagaggaaac aggccgcggt gtttacgaag ataaagcaat caaaaatatg tatgcgtccg
      301 aatatatttg gcataatatc aaacatgata aaacagttgg tgtgatcaat gaagatgtcc
      361 aaaaagggtt gattgaaatt gctgaaccag tcggtgtggt ttgcggagta actccaacaa
      421 cgaatccgac ttccacaacg attttcaaat caatgatcgc gttgaaaaca agaaatccaa
      481 tcgtttttgc tttccatcca agtgcccaaa aatcttctgc agaagcagca cgagtggtac
      541 gtgatgctgc tatcgctgcc ggagctcctg aaaattgtat ccaatggatc gaacatcctt
      601 cgatcgaagc gacttctatg ctaatgaacc atccaggtat cgcgatcgtc ttagcgacag
      661 gtggagctgg aatggtcaag tctgcttact ctacaggaaa accagcttta ggtgtaggtc
      721 caggtaacgt accagcttat attgaaaaaa cagcaaaaat taaacgtgca gtgaacgatt
      781 tgatcgtttc gaaaacattt gataatggaa tgatttgtgc ttctgaacaa gctgtgatcg
      841 tggataaaga aatctatgca gcagtaaaag cagaattcca agcacaccaa gtttatatcg
      901 taaaaccaga tgaattgcaa aaattagaag atgcagtgat gaacgaagga aaatatgcgg
      961 tcaatccatc aatcgttgga cattctgcga tggaaatcgc aaaattggca ggaatcagtg
     1021 taccaaaagg aacgaaaatg ctgattgctg aattggaagg cgtaggccca gattatccgc
     1081 tatcaagaga aaaactttct ccagttcttg ccatgatcaa agctaacaac acggaccatg
     1141 cgtttgattt gtgtgaaggt atgttgaatc ttggaggatt aggacataca gcagttatcc
     1201 attcagaaaa cgaagagttg catgtcaaat tcggtcttcg aatgaaagct tgtcgtattt
     1261 tagtgaatac accatctgca gaaggcggca tcggtgatat ctataacgaa atgattccgt
     1321 cattgacttt gggatgcggt tcttatggta agaactctgt ttctcgtaac gtctctgctg
     1381 taaacttaat caatgtcaaa actgtagcga aacggagaaa taacatgcaa tggttcaaat
     1441 taccgccaaa aatcttcttt gaaaagaact ctctattgta cttagagaaa atggaaaacg
     1501 ttgaacgtgt catgatcgtt tgtgaccctg gtatggtcca attcggttac tgcgatacag
     1561 ttcgtgaagt attggcacga cgtaaaaatg atgtgaagat cgaagtcttc tcagatgttg
     1621 aaccgaaccc atcgacaaat accgtatacg caggaacgaa actgatggca gacttcaagc
     1681 cagatacagt aatcgctctt ggaggcggtt ctgcaatgga cgcagctaag ggaatgtgga
     1741 tgttctatga acacccagac acatctttct ttggtgcaaa acaaaaattc ttggatatcc
     1801 gtaaacggac atataagatc gacaaaccag aaaaaacaca attcgtatgt atcccgacaa
     1861 catcagggac tggttctgaa gtaacgccat ttgcggtcat cactgacagt gaaacacatg
     1921 tgaaatatcc gttagcagat tatgcattga cgccagatgt cgcaatcgtc gatcctcaat
     1981 tcgtcatgtc agttccagca tctgtcacag cagataccgg gatggatgtg ttaacacatg
     2041 caatcgaatc ctatgtttca gtcatggctt cagattatac acgtggattg agcctacaag
     2101 caatcaagtt agtctttgac tatttagaaa aatcagtaaa aacacctgat atggaatcac
     2161 gtgaaaaaat gcacaacgct tctacaatgg ctggtatggc gtttgctaac gcattcctag
     2221 gtatttgtca ctcagtcgca cataaaatcg gcggggaata cgggatccct catggacgga
     2281 caaatgcgat tcttttaccg catattatcc gctataatgc aaaagatcca caaaaacatg
     2341 cgatgttccc taaatatgac tatttccgtg cagatacaga ttacgcagat atcgcaaaat
     2401 tcttaggtct gaaaggcgaa acaacagagg aattagtcga agctttagca acggctgttt
     2461 atgaattagg aaaatctgtc ggcatcaaca tgagtctaaa agctcaagga gtcacacaag
     2521 aaacactaga tacaaccgta gaccgtatgg ctgaattagc ttacgaagac caatgtacga
     2581 cagctaatcc gaaagaacca ttgatctctg aattgaaaca gatcatcatt gatgcttaca
     2641 acggataagc aaaagaaagc gtagaaaaaa gataagaaaa tagatgaaaa agaacttgga
     2701 aaacgttgtt tctccaagtt ctttttttaa tttccaagta actgaaagtt acttttgtta
     2761 tttaacattt aggtcaattt tagacagttt ttgttcaaaa agcgatattt atagtggacg
     2821 gtattactgc tatattaagg atataaaatg aaagcgttta aaaaggtggc gtaaagatga
     2881 ttattaaaga agagattttt gtacctaaag aattagtgaa agaaaaaatt gacctgctcg
     2941 ttgaaaacct gacaacgatc aaagacgata atggtgaatt tttgttggac tttgatggac
     3001 tgaaagtaga tgacaaaagc tggacagtgt ggaattggcc acaaggtgtc ggtctatatg
     3061 gtatttataa aaattaccag atgacaaaaa atcctcgcgc ttatcaagtg gtcaatgaat
     3121 ggtttgagga tcgtatggaa gaaggcgctc cgcccaaaaa cgtcaataca atggcaccgc
     3181 tattgactat ggcttacttg tatgaagaca cgaaagattc acgctatttg ccttatttag
     3241 aacaatgggc agaatgggtc atgaatgaca tgcctcggac aaatgaagac gggttacagc
     3301 atgccacata tggaccggaa aataagaatc agttgtggga cgatacattg atgatgacag
     3361 tgcttccttt ggcaaaaatc gggaaactgt tgaatcgccc agaatattta gaagaagctc
     3421 gttaccagtt tatgatccat atcaaatact tgatggataa acgaacagga ttgtggtatc
     3481 acggctggac gtttgaagga aatcataact atgccgaagc attttgggct cgtggaaact
     3541 gctggttgac gattgctatt ccagagatca ttgaaatctt agaattatcg aaagaagatg
     3601 cattgcgcaa attattgatc gaaacacttg aagcacaagt gcgtgcatta aaaacttatc
     3661 agtctgaaag cggtttatgg catacgttgt tggatgatcc ttcttcttac gtagaatctt
     3721 ctgcaactgc aggatttgct tacggtattt tgaaagcggt ccataaacgt tatttaccac
     3781 aagaatataa agaagtcgct tatcgtgcta tccaaggatt gcttgaacaa atcgatgaaa
     3841 aaggagaagt tcaaaacgtt tcaatcggga caggaatggg ggacagttta gatttttatc
     3901 ggaatatcgg aatcacagca atgccttatg gacagtcgtt aacggtgctt tgtttgacag
     3961 aattgctcgt ctcttattgt taaaagaaag gaaacccata tgaaaaaaac atcactgctg
     4021 tttgcagcaa gttcgcttag cctaggtctg ctaggtctaa gcggctgcgg aaataattct
     4081 agtgtggcaa ctgtcgcaga tgcagatgta aatgagatag ttaccttgcg ctttgcttat
     4141 gcaagcaaca gccagccagt aattgatgca atgaatgaat ttgggcgttt agtaaaagaa
     4201 aaaacagatg gttccgtaca gatccagtat ttcccagatg gacaactggg tggagaacgt
     4261 gaactgatcg aattgacaca gtcaggagca gtagatttca caaaagtaag tgcctcagcg
     4321 ttggaaagtt tctctaaaga ttattcgatt ttttcagttc cttatctatt caatgatgaa
     4381 gaacatttct ttaaggtgat ggataatgaa gaaataatgg agccaatcta ccaatcgacc
     4441 aaaaatttag gatttaccgg attgacctat tatgattctg gtcagcgcag cttttatatg
     4501 gtagacggtc cgatccatac gccggaagat ttgaagggaa agaagatccg tgtgatgcaa
     4561 agcgaaaccg ctatcaaaat ggtagagctt ttaggcggtt cgcccgtacc tatgggaagt
     4621 gatgaagtgt acacgtctct tcaatccaat ttgatcaacg gatcagaaaa caatgaattt
     4681 gtcttgtata cagctggaca tggtggtgtg gctaaatact attcctatga tgagcataca
     4741 cgtgtgccag atatcatcat catgaatgat gccatcaaag aacagctaac tgatgagcag
     4801 gaaaaagcca ttgaagaagc agcgaaagaa tcaacggttt ttgaaattga agcttttgca
     4861 aaagcaattg aagaagaaaa agctttagca caagaagaac atggagtcca attcaatgat
     4921 gtagataata caccgtttcg agaggcagtt gccccacttc atgatatatt caaaaacgat
     4981 cctacctaca aagaccttta tcaaaagatc caagagcaag gagcgtagct gtgatgaaaa
     5041 tgattttaaa caaggtatta gagatcctgg gagctgtttt gcttgttggg atggtcgctg
     5101 ttgttttatg gcaagtattt tccagaacgg ttttaggaaa tccaaatacg gtcacggaag
     5161 agcttgttcg attcggtctt gtctggtttg ccatgttgtc ttccgcgtat gttgtgggac
     5221 aaaaagcaca tttagccgta acggttttaa gtgaaaaatt aactggcaaa aaaagtaaaa
     5281 ttttagaagt tgttgttcaa agtttatttc tggtatttgc tgcgatgatc atggtgtatg
     5341 gcggatggaa tgctgtcaca ttgacaatgg ggcagatctc gccttcttta agtattccta
     5401 tgggatatgt ttatctgtca gtacctgtat cgggggtact catcattatc tatagcctga
     5461 tcaatctaat cgatacatta agcaacaagc cagcaattca agaaaaaata taaaaatgga
     5521 ggaataaaat ggcaatagaa gccggattag tgttattttt tgtatttgtt gtgttgcttg
     5581 tattagggat gccgattgct ataagtattg cattctcatc gatgatgact ttattgttag
     5641 ttataccgtt tgatgtttct gcttttagtt cagctcaaaa aatggtggga agtatcaata
     5701 gtttttcgct gatcgctatt ccgtttttcg ttctctcggg cattatcatg aataatggag
     5761 ggatagctaa gaaactagtg gattttgcga tgttgttcgt cgggcgcgtg ccgggggctt
     5821 tggctcatac gaatgtgtta gggaatgctt tatttggttc catctctagc tcggccattg
     5881 ccgcttctac cgcgattggc ggagtgttga tccctcagca agtggaagaa ggatatgacc
     5941 ggaagtttgc gacagcggtc aatatcgcgt cagctccgac aggtatggtc attcctccaa
     6001 gtactgcttt tatcatgtat tcacttgttg ctggtggtgc ttcgatctct gctttgttta
     6061 tgggaggtta tctcgtaggc gctttgtggg cgctaggaat catgctagta gcattcatcg
     6121 ttgctaaaaa gaataaatac cctgttgtcg aaaaaggaca gatggctcat gctggaaaaa
     6181 taataaaaga ggcgattcct agccttctat tgattgtcat cattatcggt ggtatcttga
     6241 cggggatatt tacagcagta gaagcttcag cgatagctgt catttactcc ctgttgattg
     6301 ctatgtttta ctataaaacg gtaaaactaa aagatatacc taatatgttg tttcaatcag
     6361 tagcaatgtc agggacgatc atgttcttat tggctacttc ttccatgatg tctttcgcca
     6421 tggcgtttac cggtattcct caggcaatta gttcgttgat tatgggcgta acggataata
     6481 aatttattat tctgttatta gtcaatcttg tgttattact ggtgggtatg tttatggatg
     6541 tggcgcctgc tatcttgatt tttacaccaa tatttttacc aatcgtcaca agtgtagggg
     6601 ttgatcctgt acattacggg ttgttttcaa tcatgaatct gtgtgtaggc tccatcacgc
     6661 cgccagtagg tacaggacta tatgtcggtg caagtgtagg aggcgtaaaa gcagaggcaa
     6721 tgctgaaacc gcttcttcct ttttatgggg ttatcttagt cgtcttgttt ttgattactt
     6781 acttcccgca aatcgtcatg tggctaccca atatgctgag ttaggagaat agttatgttg
     6841 aacattacaa ttagaggaca tgatctttcc gaagtccaaa cgatagaaga tcttgcagaa
     6901 aaaacaaaag aacaagggat tcatacactg cagctcgcct taggtctttc gtttcctgaa
     6961 ataccttctg gcgcaaatga gatcaattct gggatgggaa attatgtcaa acgagtttta
     7021 gaaaagcaag aagtgaccgt aggaatttta agttgctata tcaatatgat tcatccagat
     7081 ttaacgatac gacaagagtt gctgacaaag ttcgagcagt atcttcgcta tgcttcttcc
     7141 tttggtgcgt cgatggttgc ttcggaaact ggatgtgttt tgccggaaat ccaatatacg
     7201 gaagaaaatt tcacagatga agcatttgct gaagctgtgt ccgttatccg tcgcttggta
     7261 aaagctggag aaaaatacca aatgatggtg gggatagaac caggtttgaa tcatccggtg
     7321 tattctttag caagggtaga gcagctgatc caagcagtag attcggacta tctaggaatc
     7381 attttagatc cgaccaattt gatcacaagt agtgattatc aagaacaagt ccaattggta
     7441 gaagaagctt ttgaacgttt tggtgaaaag atatgtgcgg ttcatttgaa agattttcgt
     7501 gtcgaacagg aaaaaatcgt gccggtaaat ttgggagacg gtgtgatcga gtacacaaaa
     7561 atcaaagaaa tcatcatgaa gaatcgtccg tacctgtatg tggttcttga agaaacaaaa
     7621 gatgatggga ttcgttatgg aagaagtctt cttgaataat gtaaagaaga cgttggttaa
     7681 ctaatattta gaatggaggg ggcggggcta gaagatgcca cgtctcttct tttttataag
     7741 agtatataaa agcagctctt tttgattaca aaaaacgtgg tataataaaa ttgtgcataa
     7801 gtgaacaaaa ggaggtgcgg cacatgctcc aaaagaaaat cagcggtgaa caagtcaata
     7861 cgatggtttc tgatcagtat gaaatctatc atgtcaaaga tgtcgtcagc caaaataaaa
     7921 cgatctatca tcatcatgat ttctatgaga tccatgctac attaaaggga gaagcgactt
     7981 tttatttgaa cggacgacaa tttacgatca aagcaggaag tgtgctgctc atccattcca
     8041 atgatttgca tcgtatcgtc cgccaatctt cagatgtatt cgagcgtgtc tatatctttg
     8101 tcaccccttc ttttctagaa agtcgttcca cgccatggtc taatctatcg gcttgttttc
     8161 aaccaatggg agagaagagg agtcgtgtat tgcaaataga acaggaagta ttgaagcaaa
     8221 aattatcatt tatcgaccat cctcctgata aaaaaattta cggtgaagac ataaaatatg
     8281 aacaagctct ggtagaatac ttgatttttc tcaatcaagt tgtacaaaaa gaagagagtg
     8341 cgcaagaaca gaatgctgta gttcctaatg agcgtgtgga acaaatgtta caatatatct
     8401 caaaaaattt atctcatccg ctttctttag aggaaatgga aaagcagttt tttgtcagca
     8461 aataccatat cacacgagaa tttaaaaagt acactggatt tacgtttcat cagtatgtaa
     8521 tgaagaaaaa gctgatctat gccaagcact tgttgcgaga ataccgcagc tctagtgctg
     8581 tatatagtaa aagtggtttt tcttcttatc cgcattttct gaaagcattc aagagagagt
     8641 tcggcataac gcctaaagaa tttttgaaac gtgatacaca gaaacagttg gttcattatg
     8701 aacattttga agatgaacaa tgagaatgat cactaaaaaa taaccgcagt agctgctgtt
     8761 gtttacatag aagcagaaac tgcggttatt ttatttttat ctatatattt caaaatattt
     8821 tgaaaaaatg acgtacatat tatatgaatc agctgaattt acgaaagtct ctcggcgata
     8881 gtcctgtttt tgctttgaac aggcggctaa agtaagcaaa attctcaatc cctatttctt
     8941 cggaaatcat tcgaaccgta aattctgtat accgaagaag gtctttagcg taagaaagtt
     9001 ttttgctgat caaataatcg attggtggag aaccgataaa tttggaaaaa tctttgctca
     9061 gctgatattt attcaataag aattgttgtt ctaattgatc aagtgtaatc gtttctttga
     9121 aatgttcgtc taagtattgc ttcagtttgt aaacgtaggc gggaatatcc tcttcttcga
     9181 aatctagctg atacttttgc aaaatcagtt cattcagcaa ctcgtggata gctacagagg
     9241 actggaaatc ggtacgggca ttcgtgcctt gttgcatgtg gatcaattct gtgatcaaat
     9301 ggtggatctt gttttctgat ggatctcctg ttgaatggaa ggtgttatgg ccgtcttttg
     9361 tgaactcctg ataaaatgat tggacgttct gaccattcaa atggatccaa tccatttccc
     9421 atggttcttc gctgatcgtt tgataacgct gatattcttg gcagtcaata aaaaataagt
     9481 ctccagcttt tagctgttgc tgattatcat tatatgtaag cgaacctttg ccagacaaag
     9541 taaactttat caagtaagaa ggtaaatgtg ctcgttcagt atagtaaggg attggtgctt
     9601 tgaagtaacc gatttcttgg atataaaaaa ataaattcct gcttctttgt gtaggcgtca
     9661 agatcactct ttttgagtct tccgaccatg agtggttggc tttttcccag taatccatgt
     9721 aaacactccc aacttgtcaa gataatgcaa ttatatacca aaatagtgtg aagcaaaata
     9781 taagattctg tattatgata gcattgtcaa taaatgataa cgctttatta ataaagtgct
     9841 aatcaacaat aaagtaaaag aggtggagaa atgaatccaa catttgttgc catcgatatc
     9901 ggtgcatcaa gcggacgact aatgaaaagt acgagggatg caaaaggttc tttgatcttg
     9961 gaagaagtac accggttcaa aaacggcttt cggaacaaag acggtcacga tcgctggaag
    10021 atcgattatc ttatcaaaga gattttgatt ggatttgaaa aattaaagca gacagggatt
    10081 gatcgccttt ttgcggggat cgatacatgg gcagtcgatt actgtctgat tgatgaacaa
    10141 ggggatcgga taaatgatcc gattgcttat cgggacaagc gtaccaatgg agcagtagaa
    10201 cagttttctg aaatctattc tctagataag ttgtatgagc agacagggat tcagatccag
    10261 cctttcaata ccttgttcca attgtttgct gaagaaaaag aaaatttggc aaaagcacat
    10321 aaattgttac tgatacctga ttatttaggg tatgtcttta ctggtaacat ggtgacggag
    10381 aaaactaatg catctactat gcagctgctg aatgcaggga caaaagaatg ggaaatagaa
    10441 cttcttgaaa aaatcggtgt ctcgaaggaa ttatttccac cattgacaga gccgggaacg
    10501 atactaggag atctgcaaaa agaacaattt tcaaaatatg atttgccaga tgtgaccttt
    10561 atcacagtag caagtcatga tacggcgtca gctgtattag gaacgccagg tcaaggagaa
    10621 cgttggggtt atatcagcag tgggacatgg tcgcttttag ggattgaaac aaagatcgcc
    10681 aatgtttctc gcaatgcttt ttctgaaaat tatacgaatg aatggggtgc ccataacacg
    10741 gtgcgttttt tgaaaaacat catggggatg tggttgatcc aggaagttgc ccgaatgcaa
    10801 gagtacgagt acagttatgc ggaattggca gagcttgctg caaaagaaaa accgctgcag
    10861 caagtgatgg aaatcaatga ccctcgattt ttgaatcccc caaatatgat agaagagatt
    10921 cagcaatact gttttgaaac agagcagcca gtccctgtct catcgggagc tatcgctcgt
    10981 tgtgtgtatg ataatcttgc tctttgttac gcacaagagc tgatcaaagt agaacactta
    11041 actggaacga aattggaaaa gctgcacatc gtagggggcg gatcgaataa tcgtctactg
    11101 aatcaattga cagcagatta tgccggaatc attgtagaag caggacctag tgaagccaca
    11161 gcaatcggga atgttgccat gcaaatgctc agtacaggtc agttttcgaa tatagaacaa
    11221 gcaagaaatg taatcaaaca gtcgtttcca tgtgaaactt tttacccaga tgaaaataag
    11281 caaaacacga tggaatggta cgtgaataaa atcaaaggag taatcgtatg aacacgattg
    11341 aaagaaatta cgaacaagca aaagaaaaat atgcaactat cggtgtagat acagatgctg
    11401 tactagaaaa gatacaggac atcaaaattt ctatgcattg ctggcaagga gatgatgtca
    11461 aaggtttctt gacaccggat ggcgaattga ccggtggaat catggcgaca gggaattttc
    11521 caggagccgc acgaacacct gaagagctta gacaagatct ggagaaggct tattcgttga
    11581 taccgggcaa acacaaattg aaccttcatg ctatctattt ggatacagaa gaatctgtcg
    11641 atctaaatga aattgagccg aaacattttg aaaaatgggt agagtgggca aaaaaagaag
    11701 aaatcggtct tgacttcaat ccgactttct tttcgcatcc aatgatgaaa gacggcttta
    11761 cattggctca tccagataaa gaagttcgtg aatactggat cgaacacggg aaaagaagcc
    11821 gcaaaatcgc ggagtacttt gggaaagaaa ctgggcaaac aagtatcaat aacttttggg
    11881 tacctgatgg gatgaaagat aatccgattg atcgttttac cccacgggaa cgtttaatgg
    11941 aatcactcga tgaaattttt acagaagagt tagatgaacg gtatgtacaa gatgcagtag
    12001 aaagcaaact atttggttta ggcgctgaag cttatacagt cggttcccat gaattttata
    12061 tgggttacgg gttgactcgt gacaaacttg tttgtctaga tgctggacat tttcatccaa
    12121 cagaagtgat ttccaataaa ttatcttcat tagctttatt cagcaaagga atcatgttgc
    12181 atgtttcccg tccagtgcgt tgggattcag accatgtggt tttgatggat gatgaattgc
    12241 aggaaatcgc taaagaatta gtccgtaacg aattgttgga aaaaacaaat atcggtctgg
    12301 atttctttga tgcaacgatc aaccgtatcg ctgcttgggt catcggtacg agaaatacac
    12361 aaaaagcact gctgaaagct atgctggaac cagttgagcg attgaaagaa atggagctgg
    12421 cttttgactt tacttcaaga atggcttata cggaagaatt gaaagatttc ccgtatgccg
    12481 atgtatggaa ttatttctgt tacaaaaatc aagttccagt tggtttggat tggctggaag
    12541 aagtccagga atatcaaaaa gacgtgttgg aattaagaaa ataggagtgt ggctatattg
    12601 aaaaaaatag atattttaca agcgccttat gtgaaagaaa tgatcgaaac gacgtcgaat
    12661 ctttatcgct tgggatggga tgaaagaaat ggcgggaata tttcttattt actaaaagaa
    12721 gaagagatca caccgttctt ggatcctgaa aaagtgatcc gccgcatctc gatgattttt
    12781 gatgccagcc gtttagcagg atgctatttt attgtaactg gttcaggaag atattttaaa
    12841 aatgtagcga gcgaacctgc cgagaatctt ggaatcgttc gagttgctga agatggcaaa
    12901 acactggaac ttttgtgggg gctggaaaac gaatgccttc ctacaagcga gttgccaagt
    12961 catttcatga gtcatatcgc ccgtttagaa gtagacccag ataatcggat cgttatgcat
    13021 tgccatgcta gtcatctatt agcgatgagt tttacgcatg aattagacga acgttccttt
    13081 agtcgtacgc tatggcaaat gtgtacggaa tgcttagttg tttttccaga aggaatttcc
    13141 atcattccgt ggatggttcc gggaacaaat gagattgggg aagcaacagc tgaaaaaatg
    13201 aaagaaactc gattggtttt atggccacaa catggaatct acgggtcagg aaaagacatg
    13261 gatgaagtct ttggtttgat cgagacagca gaaaaggcag cagaagtcta tacgtacgtc
    13321 aaagcgcaag gaccaatctt gcaaacaatc acggacgaaa acctgtggca tttagcagat
    13381 gcgtttggtg tcactccaaa agcaggttat ttggaggaag ttcatacgaa agcaggtgtc
    13441 tgacatggtg aaaaaagctt tttgcatgaa agtttaccca gaaaaacaag aagaatacaa
    13501 aaaaagacat gatgaacttt ggccagaaat gcgtacagca cttcgtgaac acggggtcat
    13561 cgactattcg atctatcttg atccgagtac aagtttgtta tttggttact tagtgatcga
    13621 agatgaaaag ttatgggaaa aaatggcaaa tacatctatc aatcaaaaat ggtggaagta
    13681 catggaagat atcatggaga caaatccaga tgcctctcca gtcagttggg aattgatgcc
    13741 agtctttgaa ctgtagcaac aagcaactgg gcaaccggtt gcttggggtt ctttcgtttt
    13801 tattgtgaaa aataatacaa attaggagga atagaaaatg agtaatcgaa tgattttaaa
    13861 tgaaacatcg tattttggaa aaggagcgat tgagtcgatc gtccctgaat ttcagcagcg
    13921 aggattcggc aaagcagctg tcattactga caaaggattg atcgagcacg gtatagcaac
    13981 aaaagtaaca gaacttctag ataaagcagc aatcgcttat gctttatacg acgggatcgt
    14041 acctaatcca acgatccaaa atgtaaaaga tggagtggct tttgttaaag aggcagaggc
    14101 agactgtttg atcgcaatcg gtggaggttc gccaatcgat acggcaaaag ccattgggat
    14161 tattttgacg aatccagagt tttcagacgt cattagttta gaaggtgttg ctgatacgaa
    14221 aaatccatgt ttacctatat tagctatacc aacgacttct ggaacagcag cagaagtaac
    14281 gatcaactac gtcatcacag atgaagtaaa tcaccgaaaa tttgtttgtg ttgatcctca
    14341 tgatatccca atcgtcgcgt tcatcgacag cgatatgatg atgggaatgc cgaaaaaatt
    14401 ggctgcatca acaggtatgg atgctatgac gcatgctatt gaagggttta tcacaaaagg
    14461 cgcttgggaa atgactgata tgctgcattt aaaagcaatc gaaatcatcg gccattcttt
    14521 ggaagcttct gtagatggcg atcaaaatgg gcgagaaaaa atggcattag gacagtatat
    14581 tgcgggaatg ggcttctcaa atgtcggttt aggactggta cacgggatgg ctcacccgtt
    14641 aagtgcatgg tacaacattc ctcatggcgt agcgtgtgca gcacttttgc caactgtcat
    14701 gaaatataac aaagaatata ctggagaaaa gtaccgtgag atcgctcttg ttctagggat
    14761 taaaggagca gctgaaatgt ctttagaaga tgtaagagaa gcggcttgtg gtgagatcga
    14821 tcggttgagc aaagcggttg gaatcccaga aaccatttct gaattaggtg taaaagaagc
    14881 agatattcca gcaattgcag aagatgcttt gcgagatgta tgcacaccag gaaatccaag
    14941 agaaacgaca gtagaagaaa tcattgcttt atatcaaagt ttgatgtaaa aacgaagcgg
    15001 atcttcaaca ttaaattgcc tgtaaagaga aatggtagtt tatatctgga tatctctttt
    15061 aaattgatgg tatttccgct ataatttttc ttataaagaa ataggagaat attatatgga
    15121 aaataaaaag ccttatggaa ctgtattgat caaagcctct aaaatactag actatttagc
    15181 ggaaaaccaa gatgtctctt tgcagacgat cgcaaagggt gtgggaatga cctcttctac
    15241 agtcttgaaa atactagata ctttgttgat gatcggctac gtaaataaaa attcagagaa
    15301 aaactatcgt ttaggtgcaa agcttattcg ctacgcgaat aaaaacattg aacaaatcga
    15361 tttggtagag ttgactttac cgtttttaga acagctgcag caaaagatcg atgagactat
    15421 ccacttaggg gtattggaca acaatgagat cttgtacgtg aacaaactag atccaaaaca
    15481 tcaaacgatc cgcatgtctt caaaaatcgg aatcacccga ccattataca attctgcgat
    15541 gggcaaagcc gtattagcag aattcagtga agagcagtac actgattatc ttgaaaagca
    15601 tccgttgatc ccttacacag aatatacgat caccaatgca ttacggttga aaaaggaaat
    15661 caaaacggta aaagaaaccc acatcgcatt tgacgatgaa gaaatcgaac gtgatatttt
    15721 ttgtatagga gcctcaatcg tgaaaaacga acaaatcatt ggtgcattta gtgtaagtat
    15781 gccgaaatat cgtttaacag atgagacaaa agaacaaata atcagtgccc tcaagcaaac
    15841 aaaaagcgaa atcgagcaaa gaattcaaaa ataatgaaaa aagcgtttgc aatttttctt
    15901 ttctgccgta taatatgggt atagatttcc aagttataaa ataaaatttc taaataggaa
    15961 ataggtggag aagatgaaaa aaattgatat tttatctcga ttgaaaaacg ccggagtgat
    16021 tgcggtggta cgcggaaaaa gcaaagaaga agctttgaat gcttgccacg caatcatcaa
    16081 aggtggtcta actggtattg agttgacttt tacagtccct caagcagatc aagtaatcaa
    16141 agaacttttg tcattctaca aagaccaacc agaaatagtg attggcgcag gcacagtatt
    16201 agacgctgtc acagctcgct tggctatctt agctggcgca gagtatattg ttagcccttc
    16261 ctttgatcaa gaaacggcag agatgtgcaa tctgtatcaa attccttact taccaggatg
    16321 tatgactatt accgagatca aaacagcgct gaaaagcggg gtagatatcg tcaaactatt
    16381 tcctggaagc gcttatggac caagtatcat ttctgccttc aaggcgccga tgcctcaagt
    16441 caatatcatg ccaactggcg gtgtgagtct ggacaatatg aaagagtggt tcgatgctgg
    16501 tgtcgtaaca gtcggagtag gtgggaacct tttagcaccc gcagctacag gagattttga
    16561 caaagtaacg gaagtcgcgc aacagtatgc agcaaaaatg aaagaaatca agaggtagct
    16621 gcttatggga aaagtagtga cactcggaga aatcatgttg cgattgtcca cagagcaggg
    16681 aacccgtatc gattcaagtg aacgattcgc tgcccattat ggcggagggg aagcaaacgt
    16741 agcaatttct ttagctaatt acggacatga agcgattttt gctacaaaag tacctgataa
    16801 cgcattggga aaagcagtca aaaagcactt gcaatgttat ggagtagata cgaaacaggt
    16861 attatctggt ggatcaagac taggtactta ttatatggaa actggtagtg gcgaacgcgc
    16921 cgcctcagtg atctatgatc gtgccggatc aagttttgct gaaatggatc atttggaatg
    16981 gtcgctagag gagttattcc aaggaataga tatatttcat gtttcaggaa tcaccccagc
    17041 tttgtctaaa aaatggcaaa aaatgacgaa acaggtgata gaagctgcaa aaaaagctgg
    17101 ctgcctgatc agcttcgata tcaactatcg tggaaaatta tggacacagc aagaagccag
    17161 cgaagtaatc catcagcttt tacctttagt agatatctgc tcagctggaa aaatggatgc
    17221 tctttatttg ttaggtatcg agaaagcgcc agaagcagaa gaacaacctc taatttatta
    17281 ttatcaaaaa atgcaggaaa agttcccgaa tattcaggtg ttttattcga ccaagcgtac
    17341 cgttcattca gcaagtgaaa atgaattgaa ggggacattg tggatgaacc atgaatatgt
    17401 ggaatcccaa gtccatagaa tcaccccaat cgtggatcga gtaggtggcg gagatgcttt
    17461 tgcaggtggc gtgctccatg ggattttatc caatatgtct ccgcaagaaa tcattgattt
    17521 tgctacggca gcttctgcct tgaagcatac gatccatggg gactgcaacc aatttagtca
    17581 agaagaagtc gcacaatttt tagcatgcgg ctctggaaaa atcattcgat agaaggagta
    17641 gaagaaaatg caagaaatgg aaacacgtta tacacacagt ccagaagata tccgtcatta
    17701 ttcaacggaa caattgagaa aagaattttt agtagaaaag gtatttgaac cagggaaaat
    17761 cagtttgact tatacgcaca acgatcgaat gatctttggt ggagtgacac ctactgaaaa
    17821 agaattagaa atcacattag acaaagaact aggtgttgat tacttcttag agcgtcgtga
    17881 actaggtgtg atcaatatcg gtggaccagg attcatcgag atcgatgggc aaaaagaaga
    17941 aatgaaaaaa caagacggat actatatcgg aaaagaaaca cgacatgttg tattttcttc
    18001 tcaagatgct gaaaacccag ctaaattcta tattagttct gttccagctc atcataaata
    18061 tccaaatgta aaaatcagta tcgaccaagt aaaaccaatg gagactggtg aaggcctgac
    18121 actgaatgaa cgtaaaatct accaatacat ccatccaaat gtatgcgaaa gctgtcaatt
    18181 acaaatgggt tatacgattt tagaaccagg aagcgcttgg aatacgatgc cttgtcatac
    18241 acatgaacgt cgtatggaag cttatgtcta ctttgattat gccaatgaag atacacgtgt
    18301 gttccacatg atgggcaaac cagatgaaac aaaacattta gttgtagaca atgaacaagc
    18361 cattatttcg ccaagctggt cgatccattc aggtgtaggt acaagcaatt actcattcat
    18421 ctgggcaatg tgcggagaaa atatcaccta tacagacatg gatatggtgc aaatggatca
    18481 attaaaataa gcataaggga gagaaaatga tggaatttag tatggatatg tttcgtttag
    18541 atggaaaagt agcattagtc accggtgctg tctatggtat tggatttgag attgcacgct
    18601 ctttagctac agcaggagca aaaatcgtat ttaataactt gaatcaagaa tcagttgatg
    18661 aaggtatagc gcattataaa gaagcaggca tcgatgcaaa aggttatgtc tgcgatgtga
    18721 ccgatgaaga agcggttcaa gcgatggttc aacagatcaa agaagatgta ggttcagttg
    18781 atattctagt caacaatgca gggatcatca aacggacacc gatgatcgaa atggatgcgg
    18841 cagacttccg ccaagtaatc gacgtggatt taaatgcccc attcatcgtt tcaaaagcag
    18901 ttattccgga tatgatcgaa aaaggcggcg gtaaaatcat caatatctgc tccatgatga
    18961 gtgaattagg acgcgaaacg gtcagtgctt atgctgcggc aaaaggcgga ttaaaaatgc
    19021 tgacgaaaaa catcgcttct gaatacggtc aatacaatat ccaatgtaat ggaattggac
    19081 caggttatat tgctacaccg caaactgctc cattacgcga aacacaagaa aacggtgaac
    19141 gtcatccatt cgatcaattt atcgttggac gtacgccagc agcacgctgg ggcgagccaa
    19201 tcgatttggc tggaccatct gtcttcttgg cttctagcgc atctgatttc gttaacggcc
    19261 atgtcttgta tgtggacgga ggcatacttg cctatatcgg aaaacaacct taaaagagct
    19321 ttagtacact gtttagtgga gaggttgtga aatcagcgga ttgaacgaat accgaaggac
    19381 agctgattaa acatcattta ttcgaaaaaa gaggctggga cagaagcatt taactccaag
    19441 aaataagaag gaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc
    19501 gaaggagttg cttttacttc cgccgtttgt acgtgtttag agcgtgagac aaaagtgttc
    19561 tttacttttg tcccacgctc ttttctgtgc gtcttccata cttcactaag tcgatccctc
    19621 ttgcttcgca ccaatccttc actggtccgg aaaagatcag agattgttgg tgtagggccg
    19681 cggtattcgt tgcgccgacc atagtataaa gcagacgaag ctcttcttgc cattgcttca
    19741 tcagtataat agtttcttct acgccgtgat tcatcagatg cgtcagtacg gttcctgaag
    19801 tacccacggc atttgcgccg agacaaaggg ctttaaatat gtcataggca ttgcggatac
    19861 ctccggaggc taggatttcc atagaagtgt ctgcttcatt tgcttcaagc aatgaagaga
    19921 ctgtcgattg cccccagtca gctaaataac tcagttcccg cttggagcga cgggcgtttt
    19981 cgatctgggt gaagcttgtt ccgcttcgac cgctgatatc aactgtatga actcctaaag
    20041 cagctagatc attgagcgtt tctcttgtca tgccaaaacc gacttctttt acgatgaggg
    20101 ggacgtcaat ggctgtttga gtttcttgaa taagtacttt ccaattggta aaatcacggt
    20161 ctccctcagg cataaccaac tcttgaggtg cattcaaatg aatctgtaag gcatctgcgt
    20221 gaaacaatcg gatagcttct ttggctcttt cgacagaagt ccctgcaccg atgttggcaa
    20281 tgattttccc gtgaggatat tcttgtcgca taatcgtata agtatcggct aaagaaggga
    20341 ctttcaaggc agcgctgact gaaccagtgg caatcatcaa atctgcttct cttgcgacga
    20401 ttgcaagatc ttggttgatt ttttttgttt tttcgcttcc gccagtcatc gcattgatat
    20461 aaaatgggct gcttagtgta aacccaactg cctgtgtact tagatcgaca tcagaaactg
    20521 ctatttgcgg caaagggtta tggatgatcc gtacgaagtc aaattcgttt ttttgtttat
    20581 catgaaaggc tttagctagt gaaacatgtt cgtcttttcg attcatcggt tttcctttct
    20641 ttgatcgcta tagacatgta acggcagtgg agtgatttct gctttttccc atgcactcat
    20701 taaaggaaga atgccagatt tctggtcaac aattacgatt ccgcaatcgc ccccacctgc
    20761 accagaagat tttgcggctc cttcatactg ttcagctaaa ttacacaatt tgttcaaagc
    20821 aggcgtttcg atgacgaccc cagtgattgc agataaatca tgcagtaatt gtcggttttt
    20881 tcgaatcatc tgttggatca acgttacatt attttcttta aaccctttga tcatttcgtt
    20941 gacacattct gtactatttt ttaagaaaag ctgataagcc accattttat cttctctcga
    21001 acggtgaact tgatcgacca aatcagaagt agaggcaggg ctacccgtcc aaccaatcaa
    21061 taaacgtaaa tcttcaggag caatcaatgg ctcaatggat agacctggcc aatccagggc
    21121 aagtaactca ctgatagaat gctgagtttc ttgttcttgg agccaaggat ggtcgaaggt
    21181 tgagaaagcg atccagccac cataacagct agcagcgatg tcgccgcagg aaccattatc
    21241 ttgaactgct aaattggcta gtgctgcaat cttgaaaatc tccaactgag ataaattcaa
    21301 cgcgtaaaaa acattcaagg ctttgaccgt tgcaacggtc acggcaccgc tagaacccaa
    21361 tccatatttc cggccatttg aactgtctaa ctcacttgtt actttcagat cataaaagga
    21421 taaaagaatg tttttttctt gtgcatactt ttcagtcaag cgaatagcag caaggatata
    21481 atgaaaagga ttttcccgaa tatctaaaac taattctccg ttgcgtcttg tccaacgtac
    21541 aggcatccca ctatattgtg cagattggat acttccgact tttcgtgcag attctacagt
    21601 gactgtcacg aattgatcga ctgcagcgat aactgctgga tggcctgttt caacaactgc
    21661 atattctccg gcaatataga gtttgcctgg tgcagatact tcaatcataa aaatctccct
    21721 ttattcaata atcgcaattc ctggaccagc atgtgcagta attacttgtt ctttgctaaa
    21781 taatttggta aatgtttctt gtacctcaga taaatggctg ttttccacta aaactttgaa
    21841 attagggcca gcatccatcg taaagtagca tggaatgcct tgctttctca attgtcgcac
    21901 cgcatccatc gcttttaagc tgtcaggaga ccagtaagtg aatggaggct gggcagctaa
    21961 agtcgttcca tgcattttta gtccgtttct ttccatcgtt tctccgagaa gttggaaatc
    22021 ttttgttttg attgcttgtt tcaattgata tagatcccct tcaacggaat ctaaccagcc
    22081 ttgataaaaa ctggatgttt cgactgtccg acgcatccca tttctgctgg acacttcttt
    22141 tttctggtcg ttgatcaaaa cgaaaaccat tgctaaatcg tcttcgaaag aatcggaagg
    22201 gactggctta gcgtaagaac ttaagtcgtc atggcctttt tcccattcga cgaaaccacc
    22261 gaaaatactt cggcaagctg aaccagaccc gcgtcgagca aatcttgaaa gagagagatc
    22321 atctaatcca agcttaagag cagtattgca agcgcctgct aaagcagcta gcccgctggc
    22381 agaggaggct aatccagctg cggtaggaac gaaattctga ctgattactg aagcttttag
    22441 tgagaggccg gcttctttac gaaaaaggtc aagaaactga ctgacttttt ttgttgcttt
    22501 ttcgtcttgc aaggtgccat ctagataaaa ttcatctacc atatagctat cagaaaagat
    22561 gacttctgtc tctgtataaa atgcatccaa tgttaacgaa aggctgttgt tcattggaag
    22621 gatgagttct tcatttttct taccccaata tttgattaga gcaatgttgg tatatgcgcg
    22681 tgctttgcct ttaaacatag gtatgtactc ctaatccttg tatccaagtt tcagcagcac
    22741 ccgcatcttc aagtgcttga ctgatttctt gggcttttgt ttttgtttgt gctaatgcaa
    22801 tcatacagcc accgcgtcca ccgccagtta atttggcacc caatgccccg gtatcttttg
    22861 ctgtttggat caatagatta agaaaatcat tgctgattgt cagcttttcc agaagtgatt
    22921 gtgattcatc cattgtctgt gccaacgttt ctggtgaatt ttcaatgatt gcttgttttg
    22981 cttgcttcgt caagtatcct aatttttgga tcatttgtcc agtttcatga ggctgggttt
    23041 caaagaggtg agcgacatct ttgacggctt ctcttgtttg tcctttgatt cctgtatcag
    23101 cgacaatcaa aaaagcatcg atgttcaaag aaaagtagtc gaaaggatgc ccttttgtaa
    23161 aataaatcgg ctgcaagctg ctagtagcgg ctgcatcgat tccactagga ttaccatggg
    23221 cgattttttc cgaaagctgg acattttcta atagtatttc acgagacaaa ggaaatgcta
    23281 agtaatcata aaaagcacga gtgactgctg tggcgactgc agcgcttgat cccattccac
    23341 gttcagcagg aatcgtgctt tcaatggtca gttgcaagtt tttttggatg tcatgctgtt
    23401 tttttaagtg aatgaataat tttttgatat tttttaatgc atgaggagct tcggctaaat
    23461 ttcctgaata gtaggaagaa accagttgat cgtccattgc atccagctct tcaaggaatg
    23521 cggtgacttt tgttgcatag aaaggaaagg caatcgctgg ttctccataa acaaccgcgt
    23581 gctcgcccat caatatgatc tttccgcttg attctccttg gccatagttt gccataatct
    23641 aactccttct cacgtaaaag tgatttttaa gcaaaaaata aagcgcccaa aacgagttgc
    23701 gctgcttcac gaattttttt gtccactaag ggtctcaaaa aacattcctc ctttatttta
    23761 ctaaaaccat ctcagaagta aaactaagaa tacgttttct aaagaaaaaa cacttttttc
    23821 ttaagaattt tttgactttt tttcttcttt gactagaaaa aaatacaatt gacactacta
    23881 gatattgggg gtatatttaa aaaggttgga gttaaacaca atatttagtg atgaagtgag
    23941 ctggaggtaa atgagcgtga tcgttttagc aggtacaatt ggtgccggaa aatcaagttt
    24001 aacagaaatg atggctgaac actttgatag tcaggcattt tatgaatcga ttgatgataa
    24061 tgaagtcttg cccttatttt atgcaaaccc tgaacagtat gcttttttgc tgcaaattta
    24121 ttttttgaat aaacgttttg caagcatcaa acaagcaatg aaagatgaca ataatgtatt
    24181 ggaccgttcg atttatgaag attcactgct tttccatcta aatgcagatc taggaagagc
    24241 aacagaaaca gaagttcgtg tgtatgatga actgctggaa aacatgatgg aagaacttcc
    24301 ctatgctgcc cataaaaaac atccggattt attagttcat attcgtgtat cttttgatac
    24361 gatgttggag cgtatcgaaa aacgtggtcg ttcgtatgaa caattgtcct ttgatccaag
    24421 tctatacgat tattacaaag aattgaacag acgttacgat caatggtatg aagaatacaa
    24481 agaaagtcca aaaatccaaa tcgatggaga ccgctataat tttgtggaag atcctcaagc
    24541 aaaagaagaa gtcttgaaaa tgatcgaaga aaaattagca gagattcgcc aaaccaaagt
    24601 agccatttga tttacagttg ctcaaataaa gggtaaacct ttatttgagc gacttttttc
    24661 tttatactgg aaacaaatgt caggaggata gagtatgcgg gaaaaacact attttaatca
    24721 agaccgtaat ttttggatca atgtacagca aaacgatgtg ctagctgtta aaaacttaaa
    24781 agaagaatat ggcattagtg acgagatgct gacttattct ctagataaaa atgaacgggc
    24841 gcgcgtagag tacgattctt ttgatgaagc gttactgctt gtatcaaatg ttccgcacca
    24901 gcaaaaagtc gataatcatt atgaaactag tcctatcgct tttattttaa aagaagacgg
    24961 tttgtttaca tttaccacgc cgaataccga gtacgtgatt cgactaattc gatcgctttt
    25021 ggaacgaatg ccagacatga gtgtatacag tttattgttt cgtacattgt ttttgatttc
    25081 tgattccttt tttcctttaa tcgaggaagt gaatagtgaa cgtcagcggc ttaatctgaa
    25141 attaagagaa aaaacaacga acaagaatct gctgcaactt tcagatttag agatcggttt
    25201 ggtttactta gttacaggaa caaaacaaaa tgtagtactt ttagaacaaa tcaaggcatt
    25261 ggctatttat cgcaagttgt cagaaaagga aaaagaagag ttggacgatg cgctgatcga
    25321 agcacgacaa gcagttgaaa tgacgaactt agcttcgcag attttagacc agctctctgg
    25381 cacttataat aatttgctga ataacaactt gaatgacacg atgaaatttt tgacggtctg
    25441 gtcgctgatc ttgaccgttc caactattgt gacaggattc ttcggaatga atctccagtt
    25501 accctttacc cattccgttt tcggatgggg aatcgccttg atcatcagtt tggtcttatc
    25561 tatttggatg cttattgcac tgtggcggcg gattcgttga tctagtcttt gatctgcaaa
    25621 ccaagggctt gggcgatcac aagtgcttgc tcttggttga cgattagtcc cgccaaagaa
    25681 tcttgagata aagcgattgc tgtgaagaaa ttagtcgtaa agtctaatcc cttcagagaa
    25741 gttcgaaacc agttcgaacc ggtcaactga ttgtcagaca aaaaaaggtt tttccaagtg
    25801 acctcataaa attcgctgtc ttctaattga caatgatcga aggagacagc tttcaagttt
    25861 gtactactaa aggaagccat cgaagcaata caatcagtga aagtacagtc tcgaagataa
    25921 ctttcggcaa aattcgtacc tgttaattta cattgatgaa acacgacttg gtggaaactg
    25981 ccaccaatcc actccagatt ggaaaaatca catttttcaa aaatgacatt actgcattca
    26041 aaatgacgta aaactgtttt ttgcatcgta agtttttgga tatgactctc tcttaatacc
    26101 atatttttgg catcgagata gctttggtca cccccgttga tcagaagacc agagaagatc
    26161 tcctcatctt ctaaataaaa ctctcccttt gtaagaagag gaagagaagg tggaataggt
    26221 tcgatttttt tcatgtgggt tcacctcgtt tttctttagt atagcaaaga agcgggaatc
    26281 actgctagaa agtctgctaa tctaatcatt ttcagaaaag gaagacaaac tgtatgaaaa
    26341 acattgacaa aaagattagt ttttgctata ctatgatggt tgagaaataa aagcagaagc
    26401 acccgcttct cgccttagtt gataatgtca ctgggctaga tagttttcac tggttctatt
    26461 aggtgaagta cgcgcgggtt ctgtaagaga actcgttttt ttattgccgt ttattcggta
    26521 agcttttctt tctctcataa tatttggagg tgaatgacca tagcaaagga tatgatggtt
    26581 aacgacggca ttcgtgcacg agagttacga ttgatcggtt cagatggtga gcaattagga
    26641 gttaagacaa aagcagaagc attgaatatt gcagaacaag caaacttgga tcttgtgtta
    26701 gttgcccctg gtgcgaaacc accagttgcg cgaatcatgg actacggtaa ataccgtttc
    26761 gaacaacaaa agaaagaccg cgaagcgcgt aaaaaacaaa aagtgatcaa tgtaaaagaa
    26821 gttcgcttaa gtcctacgat cgacttgaat gacttcaata caaaacttcg taatgcacgt
    26881 aagttcttgg aaaagggcga taaagtgaaa gcttctatcc gtttcaaagg ccgtgccatt
    26941 acccataaag aaattggtca aaaagtcttg aatcgcttag ctgaagaaac tgcggatatt
    27001 gcaacagtgg aacaaaaagc gaaaatggac ggacgcagca tgttcttgac gctggcaccg
    27061 aaaaacgaca gtaagtaatt tgaatgacta actggctgtt ggacagccga tagtcggata
    27121 acgaaagatt ttgaggagga aatatagtca tgccaaaaca aaaaacacac cgcggatcag
    27181 caaaacgttt caaacgtact ggtaaaggcg gattgaaacg cttccgcgcg tttacaagtc
    27241 accgtttcca cggcaaaaca aaaaaacaac gccgtcaatt gcgtaaagca agcatggttt
    27301 ctagtggcga cttcaaacgt attcgccaac aactagcaaa aatgaaataa gaagtacaaa
    27361 taacagaatc tgaagattta tttttattag gaggaattat acatggcacg tgttaaaggt
    27421 ggaacagtaa ctcgtaaacg tcgtaaaaga gtgctaaaat tagcaaaagg ttactacggt
    27481 tcaaaacaca ctttatttaa aacagcaaaa gaacaagtaa tgaattcata caactacgca
    27541 tatcgcgatc gtcgtcaaaa gaaacgcgac ttccgtaaat tgtggattgc tcgtatcaac
    27601 gcagcagctc gtatgaatgg cttgagctac tcaaaattaa tgcacggttt gaaagttgct
    27661 gaaatcgaca tcaaccgtaa aatgttagct gacttggctg ttaacgatgc agcagcattt
    27721 actgcattgg ctgaccaagc aaaagaagct ttggctaaat aagctaaaca attataaaga
    27781 ggaaagaaga taacgaaaag ttatgttctt tcctcttttt ttatttacaa tcctccatca
    27841 aatgaaacag gacaataacg tataagtcta aaatagcata attaaggttt tttaattgaa
    27901 aactgaacat ttatggtcac cctttaatga aagcgctaac tacataagtt agaatgagtt
    27961 tgactgatat attagtttgc ttatcagaaa aacaaaatgg acggaggaat aacatgagtg
    28021 atattattat tggtattttg ttagttatct cattcttttt catggtctgg tactgtgtaa
    28081 aaggctacaa cttgatggtc ggctttgcca tcatggctac tgtatggatg ggattagcgc
    28141 tagtcggaaa cacgttttca ccaaacccgg cgatggaagg gcaaggagta attgatgtgt
    28201 tgacgcatat ctatacgacg gggccagctg agtacgccaa atcgatcttg gtaaatgtct
    28261 tctttggtgc ctttttcggg cgtgttctag tagatagcgg aatcgcagct accctgattc
    28321 gaaaggtagt tgaacttgga ggggacaagc caagaatcac gatgagctta ctatgtattg
    28381 taaccgctgt tatattcatg tcgatgacag gaatcggacc agtgatttct attgcagtta
    28441 ttgtgttgcc aatcttgatg tcattaggta tttcagtacc ggttgcactg ttctcattta
    28501 tgggctcgat tatggctgga atctttgcta atatcgtcaa tttcaaacaa tatcaaacga
    28561 tttatgctgg tttcaatcca gcggctgaaa gctatactta taatgactat ttccaaattg
    28621 gtatgattgg gatgattgta agcttagttg tggtactaac ggtcgctaat atatcaatga
    28681 acaaaaagaa acgctatgca atggcagcaa acgtaccagc tgaaggtggc gacgcaccaa
    28741 tgatctcatg gttagccgta ttattaccag ttttaggggt cgttctttta gatcttccaa
    28801 tcatcttagg ttttattcta gcaggtatct gggcattgct gtttactgga aaattgcgcg
    28861 gaggttataa agaaatctgt cgtcagtttg cgaaattatt tacagatggc gcagtagatg
    28921 ttgcgccaat ggttggtttc ttgatgacct tagcgatgtt caataattca gctgcgtatg
    28981 cttctcctta tttttcagct atttttggag attggatacc gcaatcacca ttagtcttgg
    29041 caatcgtatt tgctatattg acaccactag gatttttccg cggtccaatg aatctagtcg
    29101 gttcaggctc agctatttta gctgtcgtat tagctgttaa tccaacgatg tcgccagcct
    29161 ttttgttccc gttatttgca atcacaacga ttgctcctca gcatttagat attacacaat
    29221 cttgggttgc ttggggatta ggttatacaa aagtaacttc tcgagaatat atgaagaaat
    29281 ccatcccaac tggttggatc atcggagcga tcttatgcct gatcacgttc ttattgtacg
    29341 ggaacgccta gtaaacaaaa cgtcgataaa aacgattaga tgaatggagg aaaagaaaat
    29401 gaaacgtttt gtgcgaatgg gaattgatgt tggcggtaca catacgaaag cagtcgcaat
    29461 cgataatgcg acgcacgaaa tcatcggaaa atcttcagtg aaaacaaccc acgatgatgt
    29521 ccgtggagta gccgcaggtg tcgttcagtc ttttcaaaat tgtttgctgg aaaataatat
    29581 cagtccagaa gatgttgtgt ttgttgcaca ttctaccaca caagcgacta atgcactgat
    29641 tgaaggagac gtagcaaaag tcggcgtaat cgggatggca aaaggtggat tagaaggttt
    29701 tttagcaaaa cgacaaacta gattgaacga tatcgattta gggaataaga aaaaaataga
    29761 gattgtcaat gcattcttac ctgtcaaaca cttaaatgta gaccgtgttt cagaaacgat
    29821 ttcttcttta gaaagagaaa gagcggaggt cttggtttct tctatggcat ttggagtaga
    29881 taatggggaa ccagaacgag ttgtttatga agcagcatcg gtaaaatcga tccctacaac
    29941 gatggcttct gacatcacaa aattatatgg attgacaaga agaactcgga cagcagcaat
    30001 caatgcatct attttaccaa aaatgctaga tacggcaacc tctacagaag attccgtacg
    30061 agaagcaggg gtcaatgttt ctttgatgat tatgcgcgga gacggcggag taatggaaat
    30121 caatgaaatg aaaaaacgac ctgttttaac catgctttcc ggtccagcag catcagtcat
    30181 gggctctttg atgtacttga gagcgtctaa tggtgtttat tttgaagtag gcggaacaac
    30241 aaccaatatc ggggtcatta aaaatggacg tccagcaatc gattattcaa ttgtaggtgg
    30301 ccacccaaca tatatctctt cattagatgt tcgggttcta ggtgtggcag gcggttcgat
    30361 ggtacgggct aatcagtcag gaatcatcga tgtcggtcca cgttctgcac atattgccgg
    30421 tctagattat gcagtattta cggagacaga aaaaatcaaa ggtcctaagg ttgaattttt
    30481 ctctcctaaa gaaggagacc ctgctgatta cgtcaaggta gtgatggaag acggagaaga
    30541 agtgactatt acaaatacat gtgcagccaa tgtccttgga ttagtgcaag aagaacattt
    30601 ttcttatgga aatgtcccat ctgcacgaaa agccatccaa gcattagctg attattgtca
    30661 tacgacggtt gaagacatcg cagaacagat catggaaaaa tcctatgcta agattgaacc
    30721 ggtgatttta gaattggcag acaagtatca cttggagaaa gatcagattt ctcttgtagg
    30781 tgtagggggc ggcgctgcct cattgattac ttactttagt aataaaatgg gtgtgaaata
    30841 ttcgattcct gaaaacgccg aagtgatttc ttctattggt gtagcacttg caatggtccg
    30901 agatgttgta gaacgtatca tcccttcgcc aagtaaagaa gatatacgat cgttgaaaaa
    30961 tgaagcgatg aataaagcga ttgaatcagg cgctacacca gaatcgatcg aagtccacgt
    31021 agaaatcgat ccacagactt caaaagtaac ggctatcgct actggttcta cggaagtgaa
    31081 agcaaccgat ctaaccaagg agatcacaac agaagaagca ctagagcttg ctgcagagga
    31141 tatgcgatta aataaaaatg aagtttgctt attggaaaat acacctttct tttatgtatg
    31201 cggcgaacag aatcgttcaa aaaatgctgg aagtcttcgg atcatcgatc aaaaaggatt
    31261 tatcaaggtc caacgtggac atgcttcatg tatgaaaaca actgcagcca attatatgac
    31321 agctgtcgaa caactttggg aagatatggc agtttatcaa actgaattga ttgcccgacc
    31381 agaattttat ctctgcttag gagcaagagt ctctgatttt acagcgacgg atctggaaca
    31441 actacagctt ttgatggatc tggaagtatc tactatggaa ccggaggaag aagtgatcgt
    31501 cgttgccgga aatatcaaac aaacataagt tagaagagtt actaaacgaa ttacaacaga
    31561 tccccgaaga aatttgggga ttctaccagt ttcggcgaga tttattttgg aaaaagattc
    31621 ctttgtccaa gcagaaaatt cttatacaac aatcaatcga ttgtggtatc gaaacggcat
    31681 gtagcatcaa aaaaaagtac ccttttgccg atgtaggaga aatttgtgaa caaatggctg
    31741 ttccaatcgt ttcctgtgaa tcagagcaaa taaacgaacg aatcactttt gcaacttatg
    31801 cagaagatga agggattcga ttgatgacgg agccgttaga aaaattaaaa tgttcaggac
    31861 tagcttcgat ctctaaagaa acagcgcaag ctttgattat tggacatgaa ctatttcacc
    31921 acatcgaagc ttctgttaag ggaatttata cgcaaagcga aaaaattgtg ctgtggcggt
    31981 tgccttttta tacacatcaa tcaaacattc gtgcattgag cgagatagca gcgatgagtt
    32041 tttcaaaaga gatgaatcaa agcgattttt cgccttatgt attagaggca gtcttacttt
    32101 ggccatataa tgaaattcgt agccaaggga tcttagaaga aataaaaaaa atagaaaaaa
    32161 gatgtacgga gtatgattta gattacaaat aaagagactg tgacaaaagt cgtgtcacag
    32221 tccttttttt ccgaataaac gatgttctcc tgtcaactcc tcggtcacaa gtcaaaatat
    32281 tcaacaaaca tgagaagctg ttttttgaaa ttgttccttg tgactcggag atgaacggca
    32341 ggctcacgac ctcttggaaa acggtgttca aaagcttgca tctgcggtaa taagtccagc
    32401 caagttaaaa acataagaag ctatttttac ttggctgttc ttattacttg atgcagggca
    32461 gctttttcac aacctctcaa taaacggtgt gcaatcagct gaccctcggt attagctcaa
    32521 aaaaaacggc aaaatatgag gaactatttt gccgtttctt tctctaatac tcaggtcaaa
    32581 acgctgattc caaaacctct tatttgatgg gtctgtcctg aatatcttca aaataatcgg
    32641 ggtaattatg aatcacgtat tcttgctcct cgataatcat atgtaaatgc atcgtgcaaa
    32701 gaaatccgac ttcatcaaag ttatgatcaa tcattgattg atcataactt tgatgttcat
    32761 ctaatactcg accccagtct aattctgaaa tcgtcaaacg taaccatctg aaacggttca
    32821 agtgcgtact ataactttct agccaatccc aaatttctcg ttttccagca atttcataac
    32881 aagttcggtg aaaatcagta tccaaatcaa aaaaagcttt tttattttga gcgattactg
    32941 cctgatcggt ttgttctagt attttttcta gtttagcttg atcttgcttg gtcattttcg
    33001 cactgcattc ttgcaggatc ggtttttcca acttttcccg cgtataacgg gctaatgaag
    33061 cagatcggat atcgattttt gaaacataag tgccggactg tggaatggtt tttacaagct
    33121 cttgattgcg cagttggatc agtgcttctc gaatcggtgt acgcccgata gctagttgtt
    33181 cttctaacac tttatcggag attttacttc caggtgctaa ctcggaataa atgatttgtt
    33241 tacgtataga ttcataagct tgctgttgca aattattgat aaccatttaa ccttcctttc
    33301 tatgcagata taatctatta taacataaat cgcttacaaa tcgtgtgggc ataaaaacac
    33361 agtctgcaaa aaaagccctg ataggcatgc ctattagagc ctcgaaaaga aaaatattga
    33421 atcaatctgg tcgtacctct tggatcggtt tgacacgacc gccgattctt ttagcaaaag
    33481 cttcggattc ttttttgtct ttgaaaatga gcagattgtc taagtttttt tcccgagtgc
    33541 gacagccagt tttatcgatg taaccgtgca ttactttaac gacatacatg gggatcacct
    33601 gcttttttct tttcattata cgaactactc gacacaatac aagaaaaatg cttagacggt
    33661 ctttattaag aatttacatc atccaaagaa gcgtacgctt ggaataactg atctaactcc
    33721 ggattgactt tgctcagctt cactaatcgt tgcgaatcag aaagtaaaaa acagtgttta
    33781 ctactgccag tagtttttaa ttttttgtct tcagttccca caatttcgta gctaaaatcc
    33841 aaccgtgtcc ccgtatactt ttggatagtt ggatggatgc tcactttttc tccgtaatgg
    33901 atcatttctt tatagctgca atttacttcc agaacgggga tgatgatccc agattcttcg
    33961 atttttttat agggaaaccc tagatgtacc aataaggcta cccgagcctc ttcaaaccag
    34021 cggatataat tgctgtgatg aataatcccc atttgatctg tttcataata aaaaggttct
    34081 cgcacataac ctgtaaattt ctccatgact catgcctcgt tttccattga ttttttacaa
    34141 aaataattca tccatttcgg aagtgcttcg ctgatttgtg tcggcaaaac aatatattgt
    34201 tcttttgcca gctggtctcc aatgaaacta tgaagataaa tagctgctgc aatcgtttgg
    34261 tcattttgag gaaactgggc taaaaatcct gtgatcatcc cagctaaagt gtcacccatg
    34321 ccgcccgttg ccatagcagc agaacctaaa ggattggtgt agaagtgctg gtgaccataa
    34381 atttttgtct catgactttt caaaacgagc acagcaccga tttcctgttg cttctttttg
    34441 ttttctttat cccgttgttc ttgtatcggt agagaactca accgttgcca ctccatttga
    34501 tgaggtgtaa gtacagtttg ttcgggatag ggtagcaaaa gatgattagc agcaagtaaa
    34561 cttaaagcag agccatcaat caccagccat tgttcttttg attggttttc tagtacttgt
    34621 ttgaaacgtt ccaaactctt agaagaagta cccaatccgg gaccaatcaa gagtacatct
    34681 gcttgcttta ttacggaaag aaactggtca gtatccgtcc aaactacaat cattgcttct
    34741 ggcagatgac tgtgaagagc actccgattt ttttcatcag ttactacagt cgtgagaccc
    34801 gctccgctgt gtacggcagc taaagcactc ataatgattg ctccgccata ttgttcattg
    34861 ccgccaatca aaacgatacg tccaaatgtt cctttatgtg aaatttttgg acgaggtgtg
    34921 atgactcttt tcactagttg ttcgtttatc agcatctaaa aacctccttt tactcattat
    34981 agcgtatatt ttttttagag aaaaacagat aagaaaagga attgtcagat tttttgaact
    35041 atttttttct gtaatttgtg gtatcatgaa cacgaagact taaataagtg gaggattaac
    35101 tttatgacaa ttgattggca aaaagaagta gaagcacgta aagaagactt gcttgaagac
    35161 ttgaaaaatc ttttacgtgt caacagcgag cgcgatgact caaaagtaac accagatgca
    35221 ccatttggac ctggacctcg cgacgcattg aaacatatgt tagcttatgg cgaacgtgat
    35281 ggatttaaag taaaaaatgt cgataactat gcagggcata ttgatcttgg cgaaggagac
    35341 gaaacgctag ggatatttgg acacatggat gtcgttcctg caggagatgg ctgggacaca
    35401 gatccttatg aaccagtaat caaggatgga aagatctttg cacgcggatc aagcgatgac
    35461 aaaggtccaa gtatggcagc ttactatgcg atgaagatca ttaaagaact tgacttgaaa
    35521 ctatcaaaaa aagttcgctt cgtagtcggc agcgatgaag aaagcggctg gggcgatatg
    35581 gcttattatt tcgaacatga agaagaacca gattttggtt tctcaccaga tgctgaattc
    35641 ccaatcatca atggtgaaaa aggaaatgtt tctttagctt tacgcttcaa aggcgacaat
    35701 gcaggagatt atgtcttgaa atcttttgtt tccggacttc gcgaaaatat ggtgccagga
    35761 acagcaactg cagcacttca agtaccaagt gcagatgcag ctatcgcgat ggaagaagca
    35821 ttttaccaat tcatcgaagc aaatccagta agcggaacga ttgaagcgga caatacgtat
    35881 gtaaaaatcg aattagttgg taaaggcgca catggtgcaa gcccacaatc tggtatcaat
    35941 gcaggttctt tcttggcatt gttcttagat aactatgaat tcctaggatc tgctaaacaa
    36001 tttatccatg tagcagctgc ttatgtccat gaagattttt atggagaaaa actaggagtc
    36061 gcttatgaag atgaaaaaat gggcaaattg acaatgaatg ctggactatt tgcttttgaa
    36121 gaaaatggta cagaggaagc gaacttcatc aatatgaatt tccgttttcc aaaaggtgta
    36181 acagtagacg gattgcaaag tgacatcgaa caaacagtgg ggcaagaagg cgcaacagtt
    36241 actcgaggtg ctcgcgtaat ggaaccacat tacgtaccaa tggatgatcc attggtggct
    36301 acattattgc aagtctatga agaccacact ggagaaaaag gctacgaaca aatcatcggt
    36361 ggcggaactt acggacgttt gttgaaacgt ggcgttgctt atggagcgat gttcccagga
    36421 tatactgata caatgcatca agcaaacgaa tttatgagtc ttgatgactt gttcagagca
    36481 acagcaatct atgcagatgc catctatcgt ttggcgaaat aaaaatacta aaaaactccg
    36541 gctatctctg acagctggag tttttatgtt taaaaatgaa ttagtacatc ggtattctta
    36601 aaagtaattt agttcgtctt ttgatttctc aggagggatc aaaaaggcga aagcactaag
    36661 tatagcaaaa cttgtgatga tttgcgtacg atacgtaaac caataatgaa tctgtgagtg
    36721 attagctaaa atattgtacc aaatatacgg cgttacagca gctaaaacaa acaaaactag
    36781 aagttggaac ggacgccagc tatttcgttt acgataagca aaatagagaa aaacaagaca
    36841 agtaatcaat agcaataata ttgctacttt tggatacatc aatctaatat tgcttttcag
    36901 catctctaat cgatgcaacg ggtatttgtc gtttccttcc gttcgaaaga ggatttggtt
    36961 gattgcatct tttacaatgt ttttgttcaa tatgatcgat gataggatcc atttagccac
    37021 ccatgtcaaa ccatagccta atccccaaaa gataccggta aaaacaaaac tgcctacttg
    37081 tttgctcaat gaactgcgtt tatccattaa atacttattt gttacataat aaataatgat
    37141 cacaggtatc ccccaagaga tgaccggtgt tgtcaacaag tcaaaaaagt tagctaaaga
    37201 accaatgaca aagaaataca agaataattg ttggttggtg ttggagagtt gtggttttgt
    37261 taaaaatagt aatacagaca ataaagtcaa aatccaaata tttgaaaatt gcatactgaa
    37321 gaaaaaggtt gcagcatttc ctatagcaag cgaaatcaac aaggctgtcg ttaaatagat
    37381 gtcgctacac ttggttaaaa agtagctcgt caatccaatc agcagaaaaa gaatggctgc
    37441 gtaaatcatt cgaatactcc ccaaactcat ccctagcagt aaaggtcgta aaaaaagctg
    37501 ataaccatgc caataacggg gataatccgc catagacatc gcgttctcca aggagttcaa
    37561 ttcaggatct gcttttgttt ttcgtatcat caaatgatca gtaaaattat ctaaccgagt
    37621 gcctagcata tttccttgat tgatagaagg atacattcct tctttttcaa ccatctgtag
    37681 agatttttct gcattcgatt caatagagtg ctgcggaatg aaaaaagtaa ttgtcagtag
    37741 tacgcaatag ctagcgatca agagaacaaa agcaaaaaat atctttttaa atagtttcat
    37801 gagtatctcc tatatctgtt ttcatttttt ctcttttatc tagtaaaaaa ggagagagga
    37861 tataaacagg tagacctaat gctaaaacaa acacataccg taaactaaaa gccacaggag
    37921 tagcaatcat aatagtagca aaaacaagca taccgggaag aaaaaggagt aaataccgat
    37981 attttttctt aaagattgct aaaagtgcac tgccgatagc taaccacagt aatgtgccgg
    38041 aaccaaagaa atcacgagca tcgatgattt ttttcattcc tgacaatcca gtaagatgtt
    38101 caatcaaatc tacttgcttc ataccataat tattcgtgtt tacacgagta tctaaaaagc
    38161 cgtaactatt ttttgtacca ggtacccaga acccgaacgt ttctaaaata taagcatctg
    38221 tatagatttt tatattactt ggtaaatttc ttgcccatag tgatagaaat tcttttttat
    38281 gtgtattcaa aaactcattg ttaaaatttt tatgccactt gattgagtct gcaagactag
    38341 gagaatacgc ttcataatct tttatcggga gtagctgatt catgaatgct ttatctttct
    38401 tagacaagtc accatccata acaatagttc tagaaatttg ctgcaaagga acacctaatt
    38461 gttcagcagt ttctgtagga atagcataac gagccatcaa cgggtggatg agtagtccag
    38521 caatcactgt ataacccagc accacttgaa agaatgcttg ataccttttt tgtttcaaaa
    38581 taatcaagcc gcaaatcaaa cctgtgccgg ttactacata aatcccatta tttcttagat
    38641 aggtggtcag taaagcaagg atgattacgt aaatctgatt gagaatcgtt ggcttttctc
    38701 gttgaaaaat aaagtcaaaa accgctaagg aaaataaaaa gcaagcgata gagaacaaag
    38761 tatctttttg catgttcaag gcgtagattg gaaaaaccgg tgcaaatgca aaatagcata
    38821 aactaatgag taccggaaaa aaatggatgt ttctttttaa cagccagcaa agagaataac
    38881 ttatcgctga agctgttatc aatgtttgaa aaatcgaaaa taaaaataca ccgtgattga
    38941 tcgtatcagt tggtaaatca tgaacaaata gcttgaccag taaggtgaat aagactggat
    39001 gatgattgct tataggaaca tcacccatag attgggcgac cgaacttaac gaatcaggca
    39061 aaaccactcc aggatacaat gccaacagat aaggaagcca acacagaact aaaaagacag
    39121 taaagaataa aaaatatttt cttgaagaga aagcctgtgt gttttgagca gtaaaaaatg
    39181 tttccagttt tggcattgat aaataaatcg attgtaaaaa taaaaaagaa tacacaaaaa
    39241 tggagaaata aacaagaaag taaaaaatac ttccatcagc aatcgttgtc tttgtatagt
    39301 ttgctgagac atactgaaat tgtatctgtc tgccaataga aagtgtaaga gcaaaagctg
    39361 cagaaaaaaa caatcgggca atagctgcaa ctttgttttt tattgaaaac aactctattc
    39421 catattgctt aaaagcaagt agcaaaacca tggctaaacc aatgattact gttggattca
    39481 aagttcctag accataagaa gtaaaaaaag caaaacctga cgcaaataaa gttgcaaaaa
    39541 aagtaggaaa tagattctcc tttttcttat gcataaaaaa ggcctcctac ttcatttctt
    39601 tattttgata ttcgtggtag acatggtata aattcaattc atattctttc ttggcatttg
    39661 tgacgaccgt atccaaaatc aaccctgtta ctaatgaaag aagggctaaa gtcatcaatc
    39721 cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata tattcacgaa
    39781 tcacaggaat ccccatgacc aatccaaata aaaagaagaa gatggtccag atcccaaaga
    39841 aaagcaaagg tttataatct ttgcacattt tgatgatcat catgatcact ttgaatccgt
    39901 cagagaatgt attcaatttt gattcgctac cttcaggacg atcgcgataa tcgattggaa
    39961 tctcaacgaa cttgaatttt ttatctaaag catgaatcgt caattctgtt tcgatttgaa
    40021 aaccagaact cataataggg aagcttttga caaaaattcg attgaatcct cgatagccgg
    40081 tcatgacatc gcgaattttt gttttatata agtaagtaat tgtattttta accagattat
    40141 tcccaaaatc atggaaagga cgcttatttt catcaaaata ggttccattt gatagacgat
    40201 cgccaatgac catatctgct tcgccagaac gtaatttctc aagcaaacca tgtgcagctt
    40261 ctgcaggata agtatcatca ccatctacca tcaaataata atcggcatca atatcaaaaa
    40321 acatttgacg gattacattc ccttttcctt gacgtggttc tttctttacg attgctccgc
    40381 cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca taaatatctg
    40441 cttctggtaa ttccctttta aaatcttcaa taacagttga aattgtcgcc tcttcgttat
    40501 aacaaggaat gagcacggca attttttcac gtgattccat ttgtcactct ccttatataa
    40561 taaaactaat cttatttcaa taataccgta taataactac aaatactagc tattccaaga
    40621 ggaaatagcc ctatttttat aaaaatcacc cactataaaa gaaggatagg atacctctct
    40681 tttagatagt gggtgcgtat tcgttatttc atttttttga cgtattaatc tattgcgccc
    40741 ttttttcttg aaatgtctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa
    40801 acagtttcgc catcttctat ccgggaaagc ccaccccgat aagcactcga ccgaacggcg
    40861 aatttccgaa taggttcttt ttcaaaagag atccaagcac cttcgattgc gccttctttt
    40921 aaaaaagatg ttttgatatt ttcaagcact tggtctgctg ataacacttt tttgcgatga
    40981 taccaaatcg ttgaagctac cccactcaat aatcctagac cgatcccaac ggctaacccg
    41041 cctttaaaat agcttaattc gctttcttca tacatgtgga tatacctcct tcttctcact
    41101 tgctactctt tgtgtttatt ttaccacact ctcatatttt ctttgaaata aataaccgct
    41161 accagaaaaa ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc
    41221 gcagatcaat ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa
    41281 gaaaaaacat ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa
    41341 caagacgttc gcgcctatat ggaaagtcat atggaaccgc tggtagatga gttacagtta
    41401 gatggtttgg gcggcatttt tgggctgcgc caccatgaag aagcggatgc acctcgtgta
    41461 atggtcgcag cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg
    41521 ctttttaaag tcgtacctct aggcggatgg aacccttacg ttgtttctgc tcaacgtttt
    41581 acacttaaaa cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat
    41641 ttattacgag gaacaagcgg acaaaaatca gtagaagtaa cagatatttt gttcgatgct
    41701 gggtttgaat caagagaaga ggcaatgagt tacggtgttc ttccaggaga cacaatcgtt
    41761 ccttacgctg aaacgatcaa aacagccaac gggaagaata tcatcagcaa atcgtgggac
    41821 aaccgctatg gctgtacaat ggtattagaa gctttagaag cgttacaaaa tgaaacatta
    41881 ggtcatacat tgattgcagg cgcgaatgtc caagaagaag taggcttgcg tggttctaaa
    41941 gcttctgtca ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat
    42001 gacacagtga cgaaaaatgg cacattcgga catttaggag aaggtacatt gatgcgtatc
    42061 caagatccag gtttgatcat gcttcctcgt ttgcgtgagt acttgctaga tatcgctgaa
    42121 acaaacaaca tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct
    42181 catacacaaa atgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat
    42241 acgcatcaaa cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa
    42301 acattgaaag gtttagataa atcaacagtg aacacgatcg tatacgggaa ataacgcgat
    42361 agtgaaacta taaaatctgg caaaagcaac cttttgtcag atttttttta taaaaagagg
    42421 atcaatgttt gttagttcga aagaaaggtg tataatttaa ttgaaaaata cataaacaga
    42481 aagtagagga aaaagaatga ttatcccaaa tagtttagaa gaattagcag gctacgtaga
    42541 aactggaaaa agtgtatttt tctttactgc cggctggtgc ggagactgcc gttttatcaa
    42601 accgcaaatg cctgaaatcg aaaatgactt ttctgattgg caatttattg aagtagaccg
    42661 tgacaagtac atagatgtag ccgctgaatg gaacattttt ggtatcccaa gttttgtcgt
    42721 gatccaagac ggaaaagaat taggtcgtct agtgaataaa gatcgtaaaa caaaacagga
    42781 aatcgaatcg ttcctagaaa gtgtaataaa aggctgacat ctgttaggag gggtcaagat
    42841 gaatcaagaa tataagaaca ttttagtagg tattgacggt agtgaacaag cacttgctgc
    42901 tttcaaaaaa gcgttagaag tggctcgccg taataatggg aaagtatatg tagcaaacgt
    42961 aatcgatcag caattttata attttatggg ctatgctcct attgatcaaa atctagtcga
    43021 tcagcaaact gaaacagcca agcaaatgat cgaagaatgt aaagaatatg gaaaatctgt
    43081 tgattacaca aatatagaag gaattattgc atacggctct gttcgtgaat ccatggcagt
    43141 caaattgcca aaaaaatatg caatcgatct aatcatggtt gggcagtctg gactaaatgc
    43201 agtcgaacgc tttatgacag gaagtgtagc cagctacgtc atacgtcatt caccttgtga
    43261 tgtcttgatc gtttctgacg aagaagaaca gccaagtaat taagcaagtg gaacttgtat
    43321 aaccttttta atcaaacagt aaagggactg tgacaaaagt cttgtcacag tcccttgtta
    43381 cgaataaatg gtgttccaga agaagcttct tcggaaataa agcatgaaaa aaatctcatg
    43441 cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag gaaagaacat
    43501 gatttttgct tataacaaag cacacgtcgg agacactttg atggttatca tagccgacga
    43561 taaaggaaca gaaaacactg tcgaacgtaa atggaatgtt gctcggatca aagatgaaca
    43621 aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa ttgaagggaa
    43681 cggccaagta agcttaacag atgaagaatt agcgaaattg aatcgattga ttaaagaagc
    43741 aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg ggtatgtaaa
    43801 aacatgcgtc ccgcatcctg actctgacca tttatcgatt actgagacag aagtggacaa
    43861 tgggacagta ttgcaaattg tttgcggtgc accaaatatc gaagctggac aaaaagttgt
    43921 cgttgcaaaa ccaggagcca tgatgccaga tggaatgatg atctggccag gaactttgcg
    43981 aggggtagcc agctatggaa tgatttgttc tgctcgtgaa ttgcatttgc caaatgctcc
    44041 agaaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag tatttccaaa
    44101 ataaagaggt tgtgacaagt ctttgtcaca acctctttat tattaatttt cactattttg
    44161 attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt tttctttcga
    44221 gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca aagcagattg
    44281 aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt cgtattgttt
    44341 caacccagct ttttctgccg gtgtagctgt ttggacattt gtcacgatga caccgttagt
    44401 tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg cagataagtc
    44461 gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca gttggttgat
    44521 gatattcact acatcgttac ttgggatggc aaatcccatt ccttctacgc ttacattaga
    44581 agtcgattca gaagtacttg cgatcttact tgagttgata ccgataactt gtccttcgat
    44641 attcactaaa ggaccaccag aatttcccgg gttgatcgct gcatctgttt ggatcgcatt
    44701 gatattgact gtttcgttcg attcattcgt acttgttact tgtcggttca aggaagaaat
    44761 gattcctgaa gtgacagaat tcgcatattc agatcctaat ggggaaccaa tcgcaatcgc
    44821 aggttcacca accttcaaag cacttgagtc cccaaaagag gcaaccgttt cgactttgtc
    44881 tgaattgatc ttcaacaccg ctaaatctga ataggcatct gttccaacta attcagcttt
    44941 taccttcgta ccgtctttca tcatgacttc caaaccttgc tgtccatcta ctacgtgatt
    45001 gtttgtgact acataagcag tattcccgtc ttttttgtag attacgccgc ttccttcact
    45061 ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc cgaataatcc
    45121 gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga cagcgtcttg
    45181 aactttatct accgctttgg tgatatcaga atctacattg actttgacat tttccacaac
    45241 tgtttcaccg gcagaatttt gattgccgct tgtagcagta gaactattgt tgccagtgcc
    45301 catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc caacaagccc
    45361 cagacccaac ttacgccaga ggctattatt tttatttttt ttcatctttg gtgtcacatt
    45421 ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta gtatagtctt
    45481 atttgttgac tttagtctac tttgagactt tgaacttttt atgaaaaact ttaggaaaaa
    45541 tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatattgt gtaaaactac
    45601 gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta taaagtggat
    45661 aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta aaacaacgct
    45721 gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc tttttaacaa
    45781 aaatagtcaa aataaacata tagacgaaaa aattgattat aataaacaaa gtgagcgtga
    45841 agatcaaagg agagaaaaca tgaatatcaa aattatttca gtaggaaaat taaaagaaaa
    45901 atacttgatc caaggtatca atgagtatgt caaacggctg aatgcctatg cgaaaatcga
    45961 attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc aaatgagaca
    46021 agtaaaagaa aaagaaggag aacggatctt agcgaaaatc aaagaacaag aatacgtcta
    46081 tggcttagca attgaaggga aaaatcccac aagcgaatct tttgctaaac aaatcgacca
    46141 attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggttctt taggactcag
    46201 cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggcaaaatga cgtatccaca
    46261 tcaattgatg cgcctgatcc tcgtcgagca aatctaccgt gccttccgga tcaacgcggg
    46321 ggctccatac cacaaataat gatatttttt aagagaaaaa tagcataaaa atctagttat
    46381 ccgcataaaa actggactta tcacacttta tcaaggtcaa aaccactcaa tttactacta
    46441 atttactact tatgaatgag ctttgatacg acgatttatc cttgaaaagt gaagatataa
    46501 agatacttcc aataaaattt gaatatttaa taggtagaca cttcaaaaaa tgaggtgtct
    46561 atttttttac ccgattttga aaggaagtga acttatgaaa acaaaaaatc aagaatcaaa
    46621 aggtcgttcc ccactcttta agaccatcaa acattcattc agccaataaa aaagaaagga
    46681 taggtaaaaa tatggaactt aaatttgtga ttcccaacat ggaaaaaaca ttcggcaatt
    46741 tagaatttgc tggcgaggat aaagtcgttc agcgaagaat caacggacgg ctaactgtct
    46801 tatcaagaag ctataatctc tattctgatg ttcaaagagc agatgatatt gtggtggtgc
    46861 ttcctgctga agctggcgaa aaacatttcg gctttgagga acgtgtgaag ttagtcaatc
    46921 cacgtattac cgcagagggc tacaaaatcg gcactcgtgg ttttacaaat taccttttac
    46981 atgctgacga catgataaaa gaataaagaa agagaggaaa aatgatgaga ttagcaaatg
    47041 gcattgtatt agataaagac acgacttttg gagaattgaa attctctgct ctacgtcgtg
    47101 aagtgagaat ccaaaatgaa gacgggtcgg tttcagatga aatcaaggaa cgtacctatg
    47161 acttaaaatc caaaggacaa ggacgcatga ttcaagtaag tattcctgcc agcgtgcctt
    47221 tgaaagagtt tgattataac gcacgggtgg aacttatcaa tcccattgcg gacaccgttg
    47281 ctactgccac ctatcaagga gcagatgttg actggtatat caaggcagac gatattgtgc
    47341 tgacaaagga ttctagttca ttcaaagctc aaccacaagc aaagaaagaa ccgacacaag
    47401 acaaatagtc gctaggtaga aaggagactt tttcgcatga aacagcgtgg taaaaggatt
    47461 cgcccatctg gtaaagattt agtctttcat tttacgatag cgtcactcct gcctgttttc
    47521 ctgctggttg tcggactgtt tcatgtgaag acaatccagc agatcaactg gcaggatttt
    47581 aacctatcac aagcagataa gattgacatt ccctatttaa ttatcagttt cagtgtcgca
    47641 attcttatct gcttgctggt agcgtttgta ttcaaacggg ttcgctatga tacggttaaa
    47701 caactttacc accgtcaaaa actggcaaag atgatacttg aaaacaagtg gtatgaatct
    47761 gaacaggtca aaacagaggg tttctttaaa gatagtgctg gtcgtacaaa ggaaaagata
    47821 acctacttcc ctaaaatgta ttatcgactt aaaaatggct tgatacagat acgggtggaa
    47881 atcacgctgg gaaaatatca agaccaactc ttacacttgg aaaagaaatt agagagtggc
    47941 ttgtactgtg agctgacgga taaagagtta aaggattcct atgtggaata tactttgctc
    48001 tatgacacca tagccagtcg tatttctatt gatgaagtag aagctaaaga tggtaaactt
    48061 cgcttaatga aaaacgtatg gtgggaatat gataagctcc ctcatatgtt gattgctggt
    48121 ggtacaggtg gcggtaaaac ttactttata ctgacactga ttgaagcctt gcttcataca
    48181 gattcaaaac tgtatattct tgacccgaaa aatgctgacc ttgcggactt aggttctgtg
    48241 atggcaaatg tctactatag aaaagaagac ttgctttctt gcattgaaac attctatgaa
    48301 gaaatgatga aacgtagtga ggaaatgaag cagatgaaga actataagac tggcaaaaat
    48361 tatgcttact taggtctccc ggcacacttc ttaatctttg atgaatacgt cgctttcatg
    48421 gaaatgctgg gaacaaaaga aaacaccgca gttatgaata agctgaaaca gattgtcatg
    48481 ttaggtcgtc aagctggctt ctttctaata ctggcttgtc aacgtccaga cgcaaaatat
    48541 ttaggcgacg gaatccgtga tcagtttaat ttcagagtgg ctttaggtcg tatgtctgaa
    48601 atgggctatg gcatgatgtt tggcagtgac gtacaaaagg atttcttctt aaagcgaatc
    48661 aaaggtcgtg gctatgttga tgtaggaaca agtgtcatat cagagtttta tactcccctt
    48721 gtaccaaaag gatatgattt cttggaggaa attaaaaagt tatccaacag cagacagtcc
    48781 acgcaggcga cgtgcgaagc ggaagtcgca ggtgtggact gatcttgctg gctggtgtgg
    48841 caatagccac gccagcactt aacccctcgg tatctaacag gggggtacaa atcgacagga
    48901 aacagtcaaa aaaacattag aaaatccttt ggttacaagg gatttacaaa atttcagcgt
    48961 atgtcaaatg ggctttaaaa gttgacatac gcctttttga ttggagggat ttttactgaa
    49021 tgaacaaact tggttacagc atttaaaaga aaaacgcttg gcttatggac tatctcaaaa
    49081 ccgtttagct gttgcgactg gtattacaag gcagtatcta agcgatattg aaacaggaaa
    49141 agtcaagcca tcagaggatt tacagcagtc cctttgggaa gctctggaac gcttcaatcc
    49201 cgacgctccc cttgaaatgc tgtttgatta tgtaagaatt cgctttccga caacagacgt
    49261 acagcaggtg gtcgaaaaca tcttacaact gaaactgtcc tattttcttc atgaggacta
    49321 tggtttctat tcttattcag agcattatgc tttaggcgac atattcgtcc tttgctccca
    49381 tgaactggac aaaggagttc tggtggaatt gaaaggtcgt gggtgcagac aatttgaaag
    49441 ctatcttctg gcacaacaaa gaagctggta tgagttcttt atggacgttt tggtggctgg
    49501 cggtgtgatg aaacgccttg accttgccat taacgataag acagggattt taaatatccc
    49561 tgtactcact gaaaagtgcc aacaggaaga atgtatctcc gtcttccgca gttttaaaag
    49621 ctatcgcagt ggcgaactgg tacgcaaaga ggaaaaggaa tgtatgggaa acaccctcta
    49681 tatcggttca ttacaaagtg aagtttattt ctgtatctat gaaaaggact acgagcagta
    49741 caagaaaaat gatattccca ttgaagacgc agaagtaaaa aaccgttttg agattcgatt
    49801 gaaaaatgag cgtgcctatt atgcagtccg tgatttactc gtctatgaca atccagagca
    49861 taccgccttt aaaattatca atcggtatat ccgttttgta gataaagacg attccaaacc
    49921 tcgttctgat tggaaactga atgaagaatg ggcttggttt attgggaaca atcgtgaacg
    49981 attaaaacta accacaaaac cagagcctta ctccttccaa aggacgctga actggctatc
    50041 tcatcaagtt gccccgacct taaaggttgc gattaaactt gatgaaatca accagacgca
    50101 ggttgtaaaa gacattctcg accatgcgaa actgacagac cgacacaagc agattttgaa
    50161 gcaacagtca gtaaaagaac aggacgtgat aacaacaaaa aaataactca aatacaaatt
    50221 cattgaatat agagaggaga acatttttat gaattttgga caaaacct
//
LOCUS       DACCWK010000010        49458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCWK010000010 DACCWK010000000
VERSION     DACCWK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.010"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(243..1970)
                     /locus_tag="IVW65_000510"
     CDS             complement(243..1970)
                     /locus_tag="IVW65_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7899435.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1963..3156)
                     /locus_tag="IVW65_000511"
     CDS             complement(1963..3156)
                     /locus_tag="IVW65_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7899436.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3343..3987)
                     /locus_tag="IVW65_000512"
     CDS             complement(3343..3987)
                     /locus_tag="IVW65_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7899437.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4081..4215)
                     /locus_tag="IVW65_000513"
     CDS             complement(4081..4215)
                     /locus_tag="IVW65_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7899438.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4217..6349)
                     /gene="feoB"
                     /locus_tag="IVW65_000514"
     CDS             complement(4217..6349)
                     /gene="feoB"
                     /locus_tag="IVW65_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7899439.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6346..6819)
                     /locus_tag="IVW65_000515"
     CDS             complement(6346..6819)
                     /locus_tag="IVW65_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7899440.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7220..7444
                     /gene="nrdH"
                     /locus_tag="IVW65_000516"
     CDS             7220..7444
                     /gene="nrdH"
                     /locus_tag="IVW65_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7899441.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7603..9762
                     /gene="nrdE"
                     /locus_tag="IVW65_000517"
     CDS             7603..9762
                     /gene="nrdE"
                     /locus_tag="IVW65_000517"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7899442.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9812..10777
                     /gene="nrdF"
                     /locus_tag="IVW65_000518"
     CDS             9812..10777
                     /gene="nrdF"
                     /locus_tag="IVW65_000518"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7899443.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10866..12005)
                     /locus_tag="IVW65_000519"
     CDS             complement(10866..12005)
                     /locus_tag="IVW65_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7899444.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12314..13027)
                     /locus_tag="IVW65_000520"
     CDS             complement(12314..13027)
                     /locus_tag="IVW65_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7899445.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13281..13982)
                     /gene="nagB"
                     /locus_tag="IVW65_000521"
     CDS             complement(13281..13982)
                     /gene="nagB"
                     /locus_tag="IVW65_000521"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7899446.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14216..15748
                     /locus_tag="IVW65_000522"
     CDS             14216..15748
                     /locus_tag="IVW65_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7899447.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15939..17141)
                     /locus_tag="IVW65_000523"
     CDS             complement(15939..17141)
                     /locus_tag="IVW65_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7899448.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17223..17912)
                     /locus_tag="IVW65_000524"
     CDS             complement(17223..17912)
                     /locus_tag="IVW65_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7899449.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18018..18386)
                     /gene="rplL"
                     /locus_tag="IVW65_000525"
     CDS             complement(18018..18386)
                     /gene="rplL"
                     /locus_tag="IVW65_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7899450.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18439..18942)
                     /locus_tag="IVW65_000526"
     CDS             complement(18439..18942)
                     /locus_tag="IVW65_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7899451.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19352..20041)
                     /gene="rplA"
                     /locus_tag="IVW65_000527"
     CDS             complement(19352..20041)
                     /gene="rplA"
                     /locus_tag="IVW65_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7899452.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20145..20567)
                     /gene="rplK"
                     /locus_tag="IVW65_000528"
     CDS             complement(20145..20567)
                     /gene="rplK"
                     /locus_tag="IVW65_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7899453.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20910..21578
                     /gene="sdaAB"
                     /locus_tag="IVW65_000529"
     CDS             20910..21578
                     /gene="sdaAB"
                     /locus_tag="IVW65_000529"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7899454.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21591..22463
                     /gene="sdaAA"
                     /locus_tag="IVW65_000530"
     CDS             21591..22463
                     /gene="sdaAA"
                     /locus_tag="IVW65_000530"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7899455.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22554..23573)
                     /locus_tag="IVW65_000531"
     CDS             complement(22554..23573)
                     /locus_tag="IVW65_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7899456.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23746..24633)
                     /locus_tag="IVW65_000532"
     CDS             complement(23746..24633)
                     /locus_tag="IVW65_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7899457.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24710..25255)
                     /gene="nusG"
                     /locus_tag="IVW65_000533"
     CDS             complement(24710..25255)
                     /gene="nusG"
                     /locus_tag="IVW65_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7899458.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25356..25526)
                     /gene="secE"
                     /locus_tag="IVW65_000534"
     CDS             complement(25356..25526)
                     /gene="secE"
                     /locus_tag="IVW65_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7899459.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25550..25702)
                     /gene="rpmG"
                     /locus_tag="IVW65_000535"
     CDS             complement(25550..25702)
                     /gene="rpmG"
                     /locus_tag="IVW65_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7899460.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25864..26508
                     /locus_tag="IVW65_000536"
     CDS             25864..26508
                     /locus_tag="IVW65_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7899461.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26564..27067)
                     /locus_tag="IVW65_000537"
     CDS             complement(26564..27067)
                     /locus_tag="IVW65_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7899462.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27080..27126)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27664..28629
                     /locus_tag="IVW65_000538"
     CDS             27664..28629
                     /locus_tag="IVW65_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7899463.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28728..29900)
                     /locus_tag="IVW65_000539"
     CDS             complement(28728..29900)
                     /locus_tag="IVW65_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899464.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29887..31032)
                     /locus_tag="IVW65_000540"
     CDS             complement(29887..31032)
                     /locus_tag="IVW65_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899465.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31046..31970)
                     /locus_tag="IVW65_000541"
                     /pseudo
     CDS             complement(31046..31970)
                     /locus_tag="IVW65_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32087..33166
                     /locus_tag="IVW65_000542"
     CDS             32087..33166
                     /locus_tag="IVW65_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7899466.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33198..33791
                     /locus_tag="IVW65_000543"
     CDS             33198..33791
                     /locus_tag="IVW65_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7899467.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(33901..34128)
                     /locus_tag="IVW65_000544"
     CDS             complement(33901..34128)
                     /locus_tag="IVW65_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7899468.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34231..36819)
                     /locus_tag="IVW65_000545"
     CDS             complement(34231..36819)
                     /locus_tag="IVW65_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7899469.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36839..37498)
                     /locus_tag="IVW65_000546"
     CDS             complement(36839..37498)
                     /locus_tag="IVW65_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7899470.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37547..38050)
                     /gene="trmL"
                     /locus_tag="IVW65_000547"
     CDS             complement(37547..38050)
                     /gene="trmL"
                     /locus_tag="IVW65_000547"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7899471.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38271..39149)
                     /locus_tag="IVW65_000548"
     CDS             complement(38271..39149)
                     /locus_tag="IVW65_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7899472.1"
                     /translation="MRGNYVKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNN
                     HRFDLSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLD
                     VGCGEGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTF
                     NAVLNIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVV
                     AKFAEAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITID
                     IELLKGTKRKRFSSSF"
     gene            39245..39886
                     /locus_tag="IVW65_000549"
     CDS             39245..39886
                     /locus_tag="IVW65_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7899473.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39948..41309)
                     /gene="mgtE"
                     /locus_tag="IVW65_000550"
     CDS             complement(39948..41309)
                     /gene="mgtE"
                     /locus_tag="IVW65_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7899474.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41327..42226)
                     /locus_tag="IVW65_000551"
     CDS             complement(41327..42226)
                     /locus_tag="IVW65_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7899475.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42228..43025)
                     /locus_tag="IVW65_000552"
     CDS             complement(42228..43025)
                     /locus_tag="IVW65_000552"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7899476.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43003..43707)
                     /locus_tag="IVW65_000553"
     CDS             complement(43003..43707)
                     /locus_tag="IVW65_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7899477.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43838..44414
                     /locus_tag="IVW65_000554"
                     /pseudo
     CDS             43838..44414
                     /locus_tag="IVW65_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44532..45209
                     /locus_tag="IVW65_000555"
     CDS             44532..45209
                     /locus_tag="IVW65_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7899478.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45288..47096)
                     /gene="pepF"
                     /locus_tag="IVW65_000556"
     CDS             complement(45288..47096)
                     /gene="pepF"
                     /locus_tag="IVW65_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7899479.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47272..48270)
                     /locus_tag="IVW65_000557"
     CDS             complement(47272..48270)
                     /locus_tag="IVW65_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7899480.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48496..49149)
                     /locus_tag="IVW65_000558"
     CDS             complement(48496..49149)
                     /locus_tag="IVW65_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7899481.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14784 a        10395 c         8721 g        15558 t
ORIGIN      
        1 ttttttgaga atgcacttcg tacataataa actttaatca cattagtctt tcatacctac
       61 gatcgatctc tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttggacgt
      121 ttcgaagatg agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg
      181 agcttccaaa tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct
      241 ttttattttt tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct
      301 tgctgtggat cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac
      361 caatggaaat tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga
      421 accatcgtat ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc
      481 agatcatatt tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt
      541 tttttgatcg cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc
      601 cagtctttca agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct
      661 cctggctcga ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt
      721 acatccatgt aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg
      781 tcttcgactt gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct
      841 gaaaccatgg catctgactc gccatttgaa acagatttcc acatgattga attatctaat
      901 ggcgtcatcg ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa
      961 gcaacttctg tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat
     1021 gaaaaactcc ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt
     1081 ccttgacgtt ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg
     1141 ttcaaatgct gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg
     1201 acaaatccat tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg
     1261 ggtgcaccga tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg
     1321 atccctgcca tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga
     1381 cttgtgctcc cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct
     1441 aaattcgtaa aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga
     1501 accatcccga taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc
     1561 tgcataaagt caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag
     1621 atccctaatg gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca
     1681 gtattcatca aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg
     1741 aacaacgtaa gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag
     1801 ataaacaaag gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt
     1861 ccaatcgttt gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata
     1921 ttttctaccc agtttgccac tggtaattga tagttcaata gattattcat gttcactcac
     1981 ctcgcttgtt tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt
     2041 tccatcctct acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact
     2101 taccaccatg tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg
     2161 tttttgcttc ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt
     2221 tctgtcaaca gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt
     2281 tggtccatca gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag
     2341 cactttggaa cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt
     2401 caatatttct tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct
     2461 gtccccaatc cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac
     2521 attggcttgc aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc
     2581 cgagaacgct tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc
     2641 aacccgttgt tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa
     2701 actagaattt tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg
     2761 gacttctaac ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt
     2821 ttggaaaacc atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt
     2881 cgatacatct tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa
     2941 tagacggatc aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc
     3001 ttcttttacg tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat
     3061 ctccgttttc ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa
     3121 tatctttgtt aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa
     3181 ttcatttttc tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc
     3241 aaaaatgaaa gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata
     3301 aaaggtttag ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg
     3361 ctccgggtaa aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac
     3421 gatgtcccct gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg
     3481 agaaattgcg ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg
     3541 gtccagatgc attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt
     3601 atgtattcgc ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc
     3661 atcttgttgc ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac
     3721 atcttgatat tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact
     3781 tccatgccgt acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt
     3841 ctcaggagaa gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac
     3901 tttatatcgg ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg
     3961 gcggacgatt tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca
     4021 tcttactatg ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga
     4081 tcatcgcttg atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt
     4141 accggatttt aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa
     4201 taagaatgtc gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct
     4261 tggattttgg ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat
     4321 acgtccctaa cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac
     4381 taactaaata agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat
     4441 tcatttcccg atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg
     4501 ctaacaatga ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc
     4561 tgccattttc ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt
     4621 ctttagcgac caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg
     4681 gctgaaaaac aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa
     4741 caggttgagc ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga
     4801 taatcgttcc tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg
     4861 ctgaccattg aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag
     4921 caaacaaacg tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa
     4981 aataggcaga aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa
     5041 taatcggcaa ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat
     5101 cttgctcgtt ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa
     5161 tcagcatagg aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga
     5221 caaaagcgat cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata
     5281 taatcaattg cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga
     5341 tcagctcctg catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg
     5401 gtaaccatgt accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt
     5461 gaatagccag ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac
     5521 gattcgttgc aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca
     5581 caacacagag tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct
     5641 cactatcgtc gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct
     5701 gagaaactgc tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg
     5761 cataccagcg tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg
     5821 cttctaaacg tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact
     5881 cctgaatctt ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa
     5941 ctggtacacc aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga
     6001 tgacatccat catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag
     6061 ttaaataaag atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct
     6121 gttcctgaac caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat
     6181 agatgccggg taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt
     6241 tttctactgt tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga
     6301 acaagctagt tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc
     6361 cctttgtatc aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac
     6421 tgtcagttcg attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg
     6481 cgtcaatccc atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac
     6541 gatcccagtc tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt
     6601 ctttttattg acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt
     6661 taccagtaaa atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac
     6721 gatcccgata ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg
     6781 ataaacgaaa ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa
     6841 taaatcatgt attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata
     6901 aatgagaatc attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg
     6961 atattatgag cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata
     7021 gttttccaca attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag
     7081 ctcttttttc aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg
     7141 tttgttgttt gactttttta ctaagagttt tatactatct attatagata ataagaatgt
     7201 gttagaggag gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg
     7261 taagatggtc aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga
     7321 tgaacagcct gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat
     7381 tacttctgat gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag
     7441 ctaacatgac taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc
     7501 agtttttgtc gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc
     7561 aatcaagcat catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta
     7621 caagatgtca gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc
     7681 cctttgaata aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat
     7741 acgatgactt ttctatctat tatggaaaaa atcaattact tgatcgatca agaatatata
     7801 gaagcagaat tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg
     7861 gacgagcaac atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat
     7921 gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc
     7981 aatgcgcttt actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg
     8041 atccatcaac gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg
     8101 ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc
     8161 tcaatcaact ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca
     8221 aatcttcgtg aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt
     8281 ccagtcatga aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa
     8341 ggagctggcg tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca
     8401 aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta
     8461 cctgataaat tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat
     8521 agtgtcgaaa aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat
     8581 catctagttg caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat
     8641 gaaatctcaa aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg
     8701 aaccgcgaga atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta
     8761 caagttcaaa cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacggat
     8821 atctcatgta acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt
     8881 caatctgtgc gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat
     8941 gtcgtaccac cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg
     9001 ggcttacaca cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac
     9061 tttacaaata tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc
     9121 gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact
     9181 tatttcgaaa aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg
     9241 tttgaaggca tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg
     9301 aaagatggac tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat
     9361 atcaacgata cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag
     9421 aagaaaatcg gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat
     9481 tacacttctg cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aatggcacaa
     9541 caacacgtcg atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc
     9601 atttatgaat ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat
     9661 tacgctttcc acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca
     9721 gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc
     9781 ttattcatat tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa
     9841 ctggaatgaa attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt
     9901 ttggctagat acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca
     9961 attggaaaaa gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca
    10021 atctgaaagt ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt
    10081 tttgaacaat attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag
    10141 tacactgaat acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta
    10201 tctgcaaaaa aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa
    10261 gaaaattgcc agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct
    10321 ttattattta ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg
    10381 agatgaatct gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt
    10441 acctgaagca gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta
    10501 cgaaaacgaa gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt
    10561 caagacattc ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact
    10621 ctttccagat actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac
    10681 aagcaaccat gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc
    10741 gatgaaagac gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt
    10801 tttcgaaaaa taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt
    10861 tatgcttatt cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt
    10921 tccattcttc tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca
    10981 tagacaggta tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat
    11041 accaagatga tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc
    11101 aaatttccat ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat
    11161 ggagagataa acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac
    11221 gggataatat tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat
    11281 attctcattg ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg
    11341 atataggcag ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc
    11401 gctttcggaa agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat
    11461 agcaaaataa atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt
    11521 tttgagacac ttgcagataa actgactaat atgatattga acaaattact aagagataaa
    11581 tttgctgatg ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat
    11641 tcatttaacc atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt
    11701 gtctgttcca ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg
    11761 accaaaaacg tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca
    11821 acgattggat cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaactgcc
    11881 gaaagcattt tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg
    11941 atgatcgtga gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat
    12001 tgcatacatc tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac
    12061 gcgctgagat tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag
    12121 acgagtactt aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt
    12181 tttccaaata aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa
    12241 atataaggaa ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac
    12301 aacctcagat catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta
    12361 cggacaactc ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga
    12421 tatttttgta cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta
    12481 cgatattttt ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa
    12541 attggatttc taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg
    12601 caatagcaat cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga
    12661 tccctaatcc agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt
    12721 tcgtgacgat gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca
    12781 gccttgcttc atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta
    12841 atgcaccact agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta
    12901 aaatatgcgt acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc
    12961 cgccaaatag catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat
    13021 cattcatcta aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag
    13081 tgcaattttt acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt
    13141 atgaataaac ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg
    13201 gggagctatt tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct
    13261 ctttagccta ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca
    13321 acattgtcgt gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc
    13381 atccctttga ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt
    13441 ttcataattg aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg
    13501 aagaatcgtt tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg
    13561 tcaaatgaag caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt
    13621 tggatatcga ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga
    13681 tcttctgctt ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg
    13741 acatccatga aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct
    13801 aagtttacag aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa
    13861 gtaactggtg tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct
    13921 ttgatgattt caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc
    13981 atagtgattg ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt
    14041 acaataatta gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc
    14101 cgtgttaggt ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa
    14161 gtgattcact gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag
    14221 tatactgaca ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt
    14281 cgatctgcaa ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa
    14341 aagtacattg attaaaatca tcactggtga gatcttacca gatgacggac agatccattg
    14401 gcaaaaaaat attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac
    14461 aatcgaagaa tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc
    14521 ttttctttac caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa
    14581 actccaaacg gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat
    14641 gagcagcggg ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg
    14701 tggacaacgt tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat
    14761 cttagatgaa ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct
    14821 tcaggatttt gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat
    14881 caccacccat atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa
    14941 acaagcattg aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca
    15001 acaaaaacaa atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc
    15061 aacgatggca aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc
    15121 ttcagcaagt aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt
    15181 ggaaacaact gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac
    15241 catccgcttt ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt
    15301 attaaaaaca ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa
    15361 cacaaaaatc aattactttt cacaagatct gtactgggaa aatccattag aaacaccact
    15421 acaatattta agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc
    15481 taaagcaggg ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga
    15541 acaaactaag gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga
    15601 tgaaccgacg aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa
    15661 atttccagga acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca
    15721 acgagtcatt gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct
    15781 gtgacaaaag ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct
    15841 tcggtattag ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat
    15901 tacttgatgc agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt
    15961 ctctttcctt taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg
    16021 ctaaccgtta cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca
    16081 gccgcacctc caataaagta cataaacata aatacagtat tatttcggtt gcttgctgct
    16141 tcacctagcc cctgtactcg tgtttgattg gcgacttgac caaattgatt cccgacgtct
    16201 aataaaataa tagctaaaag caaaatggcc aaatggcttc cgccaaccaa taaagcaata
    16261 aagcttattg tttgcataac aagtcccata tacacgattt ttctttcaga ataagaatct
    16321 gccagtcgtc ccacgatagg tgcagcaact gctccagaca aaccaaaaat cgctaatatc
    16381 cctgcttcga atgttcccca attgtaggcg ggactgctga tataaaaaat caacgtagac
    16441 caaaagatcg aaaatgttcc aaacatgaaa aatccactga ctgccgcttc tcttaataga
    16501 cgttgagaag caactaaatg cggcaaactt ttaagtgaat caaggtatga aacagctgta
    16561 ctactttgct ggacagaacg cggcattttc cagtaaagca gaagtgtaag aattccgact
    16621 gcacataaag caaataaata gatcatccgc caagaaaaaa gactggcaac aataccagat
    16681 accgtccgag aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc catgaccttc
    16741 cctcttgctt ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc
    16801 gaaagtaatc ctattaaaaa agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa
    16861 gcggccaaca gagagacggc agataacgct gccattcgaa tgatcaattt tggacgatcc
    16921 accacgtccc ctaaaggaac taaaaacagt aaccctaacg catagcctaa ttgggtcaac
    16981 atcgtcaata tcccgactgc acttgtgcta acagataaac tagctgctac ttttgttcca
    17041 atcggctgga tgtaatacat attggcaaca acaacgccac aagtgattga taatagtaaa
    17101 ataagcgaag ttgttaaagg ttttttttca ttactcttca ttgatttccc ccatttccta
    17161 tcttagttta caacttatgg aaaagattgg aaatagaaaa atacaactat tcgccacact
    17221 tattatttgt gcttttgttt tccttgtttt ttcttgcgat ctttataaat gaagacaact
    17281 aaccctacta gcacagtaac acttgcaacc aacgtgactg cagttttgtt atatccgtga
    17341 aacagagctt ctccaccaca cgcatacaac acagacgaag ggatgactcc aatcacgatc
    17401 gacaatatga tttgctgtag aggcaatttc atcgcatctg cagcaaaatt gaccatagaa
    17461 gatggaataa ccggtatcat atagcctaac attacaccta tttttggatg tttcatttgt
    17521 cggattgctt gcacccaatg attcgtttcg gttttcttat ctaaaaattt taagcgatgc
    17581 ataaggaaaa ttgcagtgat gttaccaaga gcattaccca aaatgttgat gatcgcacct
    17641 gtcagtggtc caaagctcaa accagcaatg actccaacta ctgaagtagg aatacctgga
    17701 acagcacaca tcaccgctgt caatacgatc aagagtatcg atgcaaaagt cccatgcgaa
    17761 cgaatatgtg cgagtagctc tgccttactc gctctcggat ttaaaaataa ttggatgtct
    17821 gtctgatatt ctaaatacaa ccgatagccc actaccagta tcagaatgat tcccaacgag
    17881 atcaatatca atcggattat tttctttttc atatttcctc ctgaaaaaaa tctatattca
    17941 ttaaataata gtttaacata aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg
    18001 cttttaaata gtaaaaatta tttaacagtt acagatgcgc caacttcttc aagtttagct
    18061 ttcaattctt cagcttcttc tttagatacg ccttctttaa ctggtgcagg agcgccatca
    18121 actacagctt tagcttcttt caagcctaaa ccagttgctt cacgaactgc tttgataact
    18181 ttaacttttt ggtctccagc tgcagttaat tctacagtga attctgtttg ttcttcagca
    18241 gcagcagcgc caccagcagc agctacagct acaggagcag cagcagatac gccaaattct
    18301 tcttcaatag ctttaactag gtcgttaagt tctaggatag ttgcttcttt taactcagca
    18361 acgatgtttt caatgttcaa tgccattata atttcctccg tttaattgtt cttttttatt
    18421 taatgcttta tgcggcaatt aagctgcatc tccatcttct ttgtctgcca ctgctttgac
    18481 agcgtaagcc acgttgcgga ctggcgcttg tagtacagat agcaacatag atagaagtcc
    18541 ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc aatgaagaga ctttaccttc
    18601 gatgataccg cctttgattt ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt
    18661 cgctggtgcg actacatctt cgttactgaa tgcaacagct gtaggtccag tgaatacttc
    18721 gtccatgcct tcaaggccag cttttttagc tgcacgtgaa agaatagaat ttttgataac
    18781 tttcatttct acgttagcat cacgaagttg tttacgtaaa cgtgttactt catcaactgt
    18841 taatccgcgg tagtcaacga ctactactga tgcagctgct gtaaattttt cgcttacttc
    18901 atcaacgatt gctgcttttt ttgcgataat tgcttcactc atttttgagt ttcacctcct
    18961 gaattttgat ggaagagttt tttgcactga aaaggcaagt cacttttata gttactttgc
    19021 tcaaccagta ttattcatca cctattcact tcgttcatag tgattctact cgctttgctc
    19081 gaaaggaaac tcctttcgct ttgctcaacc agtattgttc atcatctctt cacttcgttc
    19141 atagtgattc acaataaaaa actctatgcc accgcagaca tagagggact attgaatata
    19201 ttcaataaac gtcctcggta ggaaattaag gcttgatgcc acctactgtc ttcggtacaa
    19261 gttaattatt aacctcaact agcttagcat gctaaactag ttgagtcaag aattattttt
    19321 cagttggatc aatcaacatt ctaatattta attagaaaga agcttggtca acatggatac
    19381 caggtccaaa tgttgttgtg atcgttacat ttttcatgta ttgaccttta gctgctgatg
    19441 gtttagcttt tagcaataca tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt
    19501 caaatgaaac ttttccgatt ggtacatgga tatttccagc tttgtccaca cggtaagtta
    19561 ctttaccagc tttcacttcg tttactgctt ttgttacgtc cattgtaact gtaccagttt
    19621 tagggtttgg cattaagcct tttggtccta atactcgtcc tagacgacct acagtagcca
    19681 tcatatcagg tgttgcaaca actacatcaa agtcaaacca tccgttttgg attttttgaa
    19741 ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc agcttctttt gctttgtctc
    19801 ctttagcaaa aactaaaaca gtttgtgttt taccagttcc gtttggtaat acaacagcac
    19861 cacggatttg ttggtctgct tttttagggt ctacgttcaa acggtatgca acttcaactg
    19921 ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg gcaacaggat
    19981 aaactttact tgaatcaact ttttttaatg catcttgcat ttttttgctc tttttagcca
    20041 ttttggttcc tccttgattg tggttataac ggttgtacct cccacgtgtt ctatatcttt
    20101 caatatagag ccaactgaga agctgctgtc ttagggtgta tagattattc tacagtgatc
    20161 cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt
    20221 aggtcttcca tttttaattc agcgatttct cttacttgat cgctagaaac tttcgcaact
    20281 ttatttttgt ttggttcgcc tgaacctttt tcgattttag ctgctttttt caatagaaca
    20341 gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac agaaataaca
    20401 accggaatga tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc
    20461 atgatattga tacccgcttg acctagtgca ggacctactg gtggagctgg agttgcttta
    20521 cctgcaggaa tttgcaattt aacgattttt tctactttct ttgccacgag acatacctcc
    20581 ttaagtccgt gatgtggtta attggagatg catatttctc ctcccacgtt ttatctgatc
    20641 tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac tggtacagta tagcaaattt
    20701 atttgttttt tcaagatttt tttctgctat ttttacattt tgcttgaata aacgctaaat
    20761 ttattctaaa aaaacaacaa tttttcgttc tcatttcttc ttttatgtta cacttgcgct
    20821 agcatggttc aacagatgaa agcaaacaat aacacggttt tttagaccct tgtacaaaga
    20881 ttcctacata acaaaatgag gtggagaaaa tgaaagattt aaggtataga agtgtctttg
    20941 atattattgg acctgtgatg attggtccaa gtagctctca cactgccgga gctgctagaa
    21001 tcgggaaaat catacgtagt atatttggcg aacagcctga ttctgtagat atttatctat
    21061 atgaatcatt tgcaaaaacc tatcgggggc acggaacaga tattgcatta gttggcggcc
    21121 tgttagggat ggaacccgat gatgaacgtt tagctgattc tttgaaattg gcatatgaag
    21181 ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa
    21241 agatgcttct aaaaaaagga acacgaaagc tttcagtcac tggcatttcg atcggcggtg
    21301 gcaatatcca gatttctgaa ttgaatggat tcaaaatttc tttaagcatg ggtactccta
    21361 ccttgatcgt tgtccaccaa gatgttccag gtatgattgc taaagtgact agtatacttt
    21421 ctgagaccaa tacgaatatc ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg
    21481 ccattatgat aattgaagta gatgatcctc aagtagaaga aacggtaaaa agactcaaac
    21541 agctgccaaa tatcgacagc gtaaattatt tcgattaagg agctaaaaaa atgtttttat
    21601 cgattgaaga attagtagat caagcaaaag cttatccaag tgtagcagaa ttgatgattg
    21661 cagttgaaat ggaaatgagt ggccgttccc gtagtcacat catcgagatc atggaaaaaa
    21721 acctctcagt aatggaacaa tcaatcaacg agggaaaaga aggtgttacc tccgtcacag
    21781 gaattaccgg tggtgatgca aaaaaattag atgattattt aaatgaggca gactttttaa
    21841 gtggagaaac aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa
    21901 aaatggggtt gatttgtgct acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc
    21961 tgatggctgt ccgagatcgg cttgatttga cacatgaaca gcaattggaa ttcttattta
    22021 ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg
    22081 gatgtcaagc agaagttggt tctgcaagtg caatggcagc agcagctttg gtttgtgcaa
    22141 aaaatgggac tcctcatcaa gcagcacaag cagtagccat tacattgaaa aatatgatgg
    22201 gactgatttg cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt
    22261 taggctcttc tcaagctttt atctctgctg atatggcatt agcgggtatc gaaagtgtca
    22321 ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt
    22381 tcaaagaaac agcagaaggt ggcttagctg ttacgccaac tgctaaacga cttgcggaag
    22441 agattttaaa taagagggcg tgataaagct ccttaatttc gagtattaag agattagtta
    22501 tcttcataac aaaaaacgaa gagaggacta attgcccttc tcttcgtttt ttattaatcc
    22561 atcaatttcg tattttttac ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc
    22621 gacaattctt tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc acctagattc
    22681 aacccataaa tcactggtcg gccaatcgca actaagtcag caccacttgc cagagctttg
    22741 aagacatgct ctcctcgacg gacaccagag tcgaaaatca ctggtacacg tttgttgact
    22801 acttcggcaa tttttggcaa tacttcaaag gacgcaggac ctccatctag ttgacgtcca
    22861 ccatgattgg atacccagat accatctgcc cctgcagaga tagcgattac tgcatcttct
    22921 ggcgattgta ttccttttac gataactgga agatgggtga tttctttgat tgttttgatg
    22981 tcatctggtg tcaagccttg ttttgcagct gcataaattt ctgcgattcc ttttccttcg
    23041 ccattaccac ttgcactttg ttcgctatac gcagctaagt taggcattgg taatggaaat
    23101 tggaattgat tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa
    23161 atgatagctt tagcccctgc ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg
    23221 tctttactca tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct
    23281 ttgattgtcg tatttgcata agtgctgatg gaaaagattg atccagcagc agctactcct
    23341 ttagctgtat cagcttcccc tttttcatgt gccaatcctt gggccgctga tggcgcttgg
    23401 ataatcggtg tatctaattc aattccgaaa acagatgtat gcaaatcggc tgagtcgatt
    23461 ccgcgtaata tccgtggcat gattttcttt gtattaaaag aagtcgtatt ttctttcatc
    23521 gtccactcat cttcagaacc accgcgaata tagccaaaag ctcctgcttc catgcgctct
    23581 tttacacgac cttctagtta ggcaatgttt acaatatcga ttggatgttc ttcattactt
    23641 gtatgataag tcatagtaga tccttctttc atcttcttga ttacatataa atcatactaa
    23701 acttacaaaa agtaagcaaa catttactct cagatacttt tatcgctatt ctttccaaat
    23761 aaatcgactt acaagctgat ctacttcttg gttttcatga agcatactat gagaggcatt
    23821 ttcacctgtc actacttttt cctctatttc atttccgcgt tgcttcaaca acgcatagac
    23881 cgctaatgca ctgtttaacg gtactgttcc atcactcaag tctgtttcat ctagttgacc
    23941 agcaatcaag aaaaatcgag tggttatagg aatattaccg atcatttgct ggtaatcttg
    24001 atagcggtta gaaacgacag caggcccctt tgctaactca tctgtcagag actgcgcact
    24061 atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa
    24121 tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca ctaactcctc ccatggaatg
    24181 ccccactatg ttgactttat ctatgccgta gttttctttc agatacaaaa gacaagtttt
    24241 gatccattct gcttgattcc actcgttatt tttattatct tcaaacaata cttggaccat
    24301 cggattgtct gcttttccag acaaatctcc tgttgcttgt atttgtccat ctgttcctac
    24361 tgttaaaacc aattctttta ctgcttgctg atttttttcc aaacgccgca acatcccttt
    24421 aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag tggttttttc
    24481 tatcacttta ctggattctg ccgttttttc tttatcagaa acttcagtag tatttatctt
    24541 cgtcgtctgg caaccactta acgcaaacaa actgataagg actaaagctg ctgcagttgc
    24601 acgtttgcat aaatttttga ttggttctgt catacacctc ttcctttcca tatagaaaaa
    24661 agaaagactt gaaaggccaa tcatatgatc gacatttcaa gtctctttat taatcgattt
    24721 tatctacttg atcgaaatca agttctgtac tggtttcacg tccaaacata tcgatgttga
    24781 ctttcaattt ttctttttct ccgtcaactt ctgttacttg tccttctaat ccagcaaatg
    24841 cgccttcgat gattttcacg acttcgccaa cttctacttc tagatcattt tgtcgcaggc
    24901 tcataccgat agaacgcaag atatgattga cttcttctgg aagtaatggt gctggtttgc
    24961 ttccggctcc gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg acaacatacc
    25021 atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga
    25081 tttctttttc tttcccattt ttgacttcag tttctttttc ttcaggaaca acgacacgga
    25141 aaataaagtc ggccattttc atactttgcg cacgagattc gatatttgct tttactttgt
    25201 tttcatatcc tgaatatgta tgcaagacat accagttttt ttcaaaagat tccattgaag
    25261 gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt
    25321 cattatagca ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca gccaaataag
    25381 ctttggatgg ctgtatccat tacgtaaaac atcaatccga aaataataga agtttcgatg
    25441 actactaacg tatctttacg gagttgtttt ctgcttggcc aagttacttg tttgatttca
    25501 tcttttacgc tttttagaaa tttcatgaga caacctccct tgctactgtt tatttcgttt
    25561 ctttatgtaa tgtatattga ttacaatatt tacaaaactt attgatttcc aaacgttcgc
    25621 cgctttttcc ttcactgact gattttgtgt agttgcgaga cccacaaacg gaacaagcga
    25681 gtgctgcttt tttctttgcc atttacttct gcccccttgc tgagaatcag acttatactt
    25741 cataacagta tcacgtaact ttttacttgt caatttgtga aataaactat ctattgtttt
    25801 ggataaaatt atgatatgat taatttgaaa ttaagctaaa atgagacaat tggaggtata
    25861 gcgatgctat taaaaacact tgtatataag aaaaaagatc tgacaaccgt tacagagact
    25921 agtacattag aagaagcatt aaaaatttta gaaacttccg gataccgttg tgtgccgatc
    25981 ttagattctt caggtaatat tttccgtgga aacatttaca aaatgcatat ctatcgtcat
    26041 aaagcaaatg gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag
    26101 ttcatttacc tggatacttc tttcttcaaa gtattcttta cgatcaaaga gcttccttat
    26161 attgctgtcc ttgacagtga aaatcatttt tatggtattt tgacacatag tacgttgctt
    26221 aatatgctct ctcaatcttg gagcgtagaa caaggaagct atgtactgac aattgcttcc
    26281 acaggaaagc aaggagattt ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt
    26341 gccagctgta ttactcttga tatcgaaaaa gacgaataca ttcgacgtac attgatcacg
    26401 ctaccggcta acactgaagc gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag
    26461 aattataaag tggtagaaat tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa
    26521 aaaagaaaac agctaatctg ctttcttttt tcgcttttta taattagaca cgcttttcaa
    26581 aatcaagcgc gcgatctaaa taatacatca atggtgctga aacagccatg acgataaact
    26641 cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt ggaactaaca
    26701 tataagcaat cagtcccatg ctgatggtaa agaagatcgt gttcaatact aaacgcatct
    26761 ttacattttt cactttgttt tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc
    26821 cgccaccaaa aaccacatcc atgataccaa aaccaaagaa ggcattatag acgatgaccc
    26881 cgcctaaaac tccccaaagc aatttacggt taaagacaac taaatgattc aaactttctg
    26941 aaatacgaaa ctggatcggc cctgaggata caggcgcaac taaaatagtt aacgctagat
    27001 aaagagccat gatgatgcca tttacaacga ttacggataa ccgttgtctg cttttcgttt
    27061 tttgcatttt ctctcctctt ttctcctagt ttttttaacg cgggatggtt gatgaaccgc
    27121 ggttatgtga aacaaacaat aattataaca gacttttcat ttatttcaat ccgtcttatc
    27181 tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg
    27241 gtataaagta agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa gtaggatctg
    27301 caagaacaga attgttgtga aacttaaata aataaaaaag gatgtgggaa tttgagcaac
    27361 ctcttactag agaacggtct agcaagcagc tgaagatcta agtggacatg ccgaaccttg
    27421 gaacttctcc ttacgtaagt aatggatcat gcgcttgcta ggcttttttg tgtttttcgg
    27481 tcttcggacc tttttcattt tcctagactt ctgctataat aaccaagaat taattttgta
    27541 acagattttt ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag cttgctaatt
    27601 attggttaac cagcaaacag gagtattaaa atatcttgtt ttattgaata gcaggaggaa
    27661 atcatgaaat tccattatca tatattgatc aatccatcgg caggcagcgg aaatggctat
    27721 aaagtagcag aaagaatttt accggtatta aaaaataagc atattgacta tacacttcat
    27781 tatagtgaat acaaaggaca cgatgctgag attgctgaaa ctttggcaaa agaaacattg
    27841 attccttgga tagaagaaga acagaaaaca atcgatactt tccccctttt gatcatcgta
    27901 ggaggtgacg gcaccttaca tcaggtactt gatactttct atcagatgga agtagaattc
    27961 ccagttgctt atattccagc aggttctgga gatgattttg ctcgaggggc aggccttcca
    28021 aaaaatccca aaaaaggact tcagctgatt ttagcagctc aatcaccaga aaaagtccac
    28081 ataatggctt atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa taatttcggt
    28141 ataggattag atgcagctat cgtccatgcg acaaatcatt cttccacaaa aaagcgattg
    28201 aacaaataca acttaggatc attttcgtat ttattctcta ttctccgtgc tttattcact
    28261 caaaaaggat ttccaattct ggtagatgca ggaggaaaac aattaagttt ttcaaacgca
    28321 tttttatgta cagcaacgaa gcatcctttt tttggcggag gaatcgcgat tgttccaact
    28381 gctgatgcgt ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa
    28441 atcttgtggc tcattctttt acttatacag aaaaaacaat tgaaatcgaa atatttccat
    28501 cactacacaa caaacagatt acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat
    28561 ggagaagaaa tggaaaaacg accttttgat atcacaataa ccacaactaa acagttgttt
    28621 tggtgttaat aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt attcggaaaa
    28681 aaggactgtg acacgacttt tgtcacagtc ctttttttac tatgcagtta attctttgcc
    28741 caattgggtt gccatcgata gtttctttct ttacccaaaa taaattggat cattgaaaac
    28801 accgcagcaa aagcaactaa gacacaacca ttgaaccaaa aagattctgc aaatgtttga
    28861 ttgtaactga gtgtttcact gatcaagtta ttattttgaa caaaccaata tgttggtgtg
    28921 aatgccgcta ttttgctaac catttctggc aagtattctg ctggtacaaa caccccaccg
    28981 ataaagcaac tgcctgtgat aaaaatatct ttgatcccac cgctggtttc tgagtttttt
    29041 accaatgaag tgatacaagc cgaaaagcta atgatcggca ataaaaacaa gaaagtattt
    29101 aaaatatgat accataccgt tatatcccac gaggtgtgga tcactaacag aaccattaga
    29161 agtaagaagc cactgaacaa cagcagcgag taactaatca aactaaaggt tactttgcga
    29221 ttcaagtttc gtttggtaat gggcgcactc tggtttcgtt tactgatctc tttgcgatta
    29281 aatgctagat tgatgacgct gaaaccgcta aaaatggata gaaacaaccc ataagacaag
    29341 aaattaaagg ttgtgcccgt caacagcttt ttcatccgct gactatatcc cgcacttagc
    29401 tcaacttctc cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt
    29461 ttatcttctt cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca
    29521 actgttttag aaaaggcatc tgggcgactc tgtatcttga tcgtcggcat ctttcctact
    29581 tcgacttgtt cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg attaaaataa
    29641 agagcatcat cgatttcttt ttgtgaggtt ccatccaatg taacgactgt ttccttgtca
    29701 ttcaaatatt tacgaaggcc atcgactaca ggcgactctt cttcactaat caaggcgatt
    29761 ttcgcatctt gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca
    29821 acaacagtga taattacacc tatcagtaaa ttcgatttat ttgctttaag caatgtccaa
    29881 atcgttttat aaagcgtcat aacgcacccc tctttccatc caagcatttc ctaacgcaaa
    29941 aaggaaaata aatccgatca accatagaag attggtataa aaactagcca aatcagtgta
    30001 gaaataaagt ttatacaaac tttcgctgat caagttgacg agatttaatt gaccaacaat
    30061 cggaatatga agatcaatcc aatacttcac ttgttccgac cccatcatac cagcaaaaaa
    30121 gctcatcgca atcgtcactg aaatcaagat gctatccttt tgcaccagat tcatttttgg
    30181 tatcaaatta ccaaccaatg tacctaacaa aatcgcacac aacgacccca gagcggtaac
    30241 aatcatcaac cattgccaac gttcaccaaa gtctacttga tagataaaac gaaagacagc
    30301 taacataata taaatctctg tcaaaacaat cgtaaagctt gctgccaaat tagcgattga
    30361 aactaacgat tgctttctag gaatcaagga caagcgaata ccatttgacg attgatttgc
    30421 ttgctgatcc tgtacattgc ggacccccca catcgtcccg tagagaatcg ccattccaat
    30481 cagagtgaag aaataaaaac ttttgaaact catattacga cctgcatgaa cttcttgaaa
    30541 agatacttct tgttcaaaaa cagtcatgat gggcgcgaga tcaacgttct ccacttctgc
    30601 cactttactc gaaattatac caatacgctg taaaaattga tccatgacgt ttttcatcaa
    30661 cgtctgactc attcctgctt gacccacatg gagcgaaaca gatgaaccat caatgagata
    30721 aaaaccagcc aaatcatctg catttagctt ttcattcgct tctacttccg atagttcctg
    30781 tacttcaaat aatggtttat cattgacttc gacttgttct aagactgtgg taaaattttc
    30841 cgaaagcgct gtttgatccg ctttgattac aattccagta ttaatcgttt cgagattgcc
    30901 accttgatca atctcgccaa aggccgccat aaacatcaat cctaaaagaa ttggaaaggc
    30961 caatgtccaa aacaacatgg atttatttcg cagcagtatt tttgtgcgat atttataaaa
    31021 atgccaaaac attatttccc ctcctctagt ctctcaaatc cttgccagta atctctaaaa
    31081 agacatcatt caaggacgct tctttggttg ctatttttcc gtaactgatc ttttcttctt
    31141 gtaagattgt aagaatcggc aacaatactg tatttagttc agtagttgtt acttggagct
    31201 gacctttttt ataagcgatt ccatcagaag ctattgttcg ctgacaacac gttttctggg
    31261 agtaatggga tgtcgatcgt caaacgatcc gccgtccgta ccatatcttt tagagattct
    31321 ttggttcctt tagcaatcac ctgtccttga tccatgatca cgatctggtc gcaaagaatt
    31381 tctacttctt ccatgtaatg tgttgtatag acaatggttg ctccttgtcg attcagctcc
    31441 ttgatacttt ctaaaatttt attccgactt tgggggtcta cagctactgt cggctcatct
    31501 aaaaaaatta accgcggctt gtgggcaatc ccacatgcga tgttcaaccg acgtttcaag
    31561 ccaccactaa gttctcctgg acgaaacgaa acatattttt ctaatcccac taattgaatc
    31621 acctcttcaa tcaaggcttt ccttttctct ttttcggaaa catataaacc gcaaaaataa
    31681 tcgatatttt cttgcacact tagttcgtca aagaccgcaa cgtcttgagg aacgacccca
    31741 atcattgcct tgacagcata attttcagga gtcattgttg tcccgaacaa tttaatggtt
    31801 cctttgtcat aatttaacaa agaaagcata cagttgatgg cagtcgattt cccactgccg
    31861 tttggtccta acagacctaa gacttctcct tcttgtactt gcagatcaaa atgattcaat
    31921 gctacaaaat ctccatagcg tttgactaat ttatcaattt cgataatcat catgaaaaac
    31981 tccttccaaa tttcttgttg atctgactat aataaagtgc aagaaaagaa actagtgacg
    32041 attggctaaa ccacacatga catttgtcac aatgaaggag tctgctatgc ataaatctcg
    32101 gctactcaac gaaactagct ttctactggt tatctcattg cttttgctgg taagtcaagg
    32161 attcgttgct atgcaagtta tctatttatt gatcgccgtt ttattcactg gtgtcagtta
    32221 tttactgcct acaaagccaa attggatttt ccaagttatt ctatttcttt tatgtgcctt
    32281 tttacctgat cttgtcctgt ttctgccagc gacgatgcga gtgatcgccc ctgaagaaag
    32341 ccaccttgat tacttgtttt tgttaggttt tttgttgatt tcgacggttg gtaatcatcc
    32401 ccccatcaag gtcgcctttg tcgtaatttt gctgtttttt tccttttact tacgaaaaaa
    32461 agatcaacga acgaccgatc ttgagaaaaa gtacattgtc tccaaagata ctcattggga
    32521 aaaacaacaa gagttatcgg aacaaaataa agcattggcc ctgtcacaag aaacacaatt
    32581 agccttgcaa attgtggaag aacggaaccg aattgcccga gacatccatg acaatgtcgg
    32641 tcatctgctg tcaagtgcga ttttacaaat tggtgcgctt gaaatgatca atcaagaacc
    32701 aaaactaaaa gaaccattag aaaatttaag tgccaccgtt cacactggga tggatcggat
    32761 tcgtcaaagt gtccatgacc tccatgagac atcgatctct tttcaacact ccttagagtt
    32821 tctgatcgct gattttcctt cttctgttac gattgaagga aacttgtttg aaggattgaa
    32881 tgaaacgcag caaaactgct tcttaatggt catcaaagaa gccttaacca acagtatcaa
    32941 acatagtcat gccaaaaccg ttgttttaca ttttcgaacg ttgccggcct tttatcgctt
    33001 gcaaatcacg aatgatggca ccagcccaca aaaaaattct gctcccggta ttggtttgaa
    33061 aacgatgcgt caacgtgttc aagaacaaaa gggacaagtc catattttcc aagaaaacca
    33121 gcaatttcag ttaacgatca ttttaccaag ggagggacac caatgataac agtagtcatt
    33181 attgagatga tccatttgtg acgaaatcgt tacaaaccat tttagaaagc acgaaagaga
    33241 tccgtgtgtt aggcattggt cattgtgcca aggatgcgct agtcttatac gaaacccatc
    33301 gtcccgatgt tttgctgacg gacatccgca tgcctaaaca aacgggcatc gatgctgcca
    33361 aagaaatttt ggctaaattt cccaaagcca tccttttgct tttgacgaca ttcaaagatg
    33421 aagaatacat ccgcgaagca ttttccatcg gtgtcaaagg gtatttaatc aaacagaact
    33481 tgcaagcgat tattccttct gtcaaatccg cttataatgg acaggccgta tttggtaatg
    33541 aaatcatcga gactttcact caaatgatga agaataaacc gacgattgat agtcatgctt
    33601 atgcttcttt tagtgaacgt gagcttgcaa tcatcaaaga agtagctgcc gggaaaaaca
    33661 ataaagaaat tgcagatgcc ttgtacttga gtgacggcac tgttcgcaat tacatcagcc
    33721 aattactaga aaagctagat ttacgggatc gcacgcaatt ggctatctat ttttatcagc
    33781 atttgcaata agtcttatct aacacaaaaa aacctgaatg atgctccttc gcatcattca
    33841 ggtttttttc acatcttcta ttcaattgtc ccgcaaacca tttcctcacg attgaaagaa
    33901 tcaaggattc gcagggctgc caggattcgt tggattgttc ggcgtgttag gcggaaaaat
    33961 aggcgccttg tctaacgttt tttctagttt tgattcttca cgatcgtctc gttctacatc
    34021 aggactttca ttcaatttga attctttttc catctcatct aactttttaa agtcattgcc
    34081 agattctttt gctaatttcg ctaatttatc ttcggtttct ttagccataa agtaacgccc
    34141 cccttttgat ttcagcttag catatcacac tgaaaaaaca aaagaaaacg ctaaccaacg
    34201 atcattttta gctatttctc atctttaaaa tcaagcattt tttgccggta taaaatcgta
    34261 gggagaactg ttttccatac caatcatcgg atctactaac tggttatcga tcacttctgc
    34321 tgtaagaacg aaatctttag aaacagcagg ttctacctct tctttctttt cttctccaac
    34381 aaatgaagaa aacagatctg gttccactgt ttccattact tggtttttcg tttcattcgt
    34441 cccactgtca tcttctacag gtagcttttc ttttacaacc cgtggctctt ttgtatagtt
    34501 cccatcttca aatggatcat ttccgaccaa agaatcttca taagctgcta gtttggtccg
    34561 gagagtgata ttcattttat cattatccat gatccttgat ttcaacgtag tcatccggct
    34621 tgctgattca ttcgctacac aagtgacata ggcttgctct tcccctaaca aaatcaacag
    34681 ttccttactg cgagtcactg ccgtgtagag caaattacgc tgaagcatcc gctgatattg
    34741 atggaccatg ggcaaaataa ccatcttgaa ttcactgcct tgtgatttat ggattgagca
    34801 acaataagaa agagtgattt tattccattc gtttctttta taagtgacct catttgcatc
    34861 aaactggata actaattcat ccactttatc ttcagaatct tttgccaaga tgatacccgt
    34921 gatttctccc atatctccat tgaatacatt caattcggga gtattgacta attggaggac
    34981 tttgtctcct atccgataaa cggtgtcgtt ccaatgtact tccttttttc caggtttccc
    35041 tggattgaat atctcctgca tcattttatt taacgcatcg atccctgcag gtccccgata
    35101 cattggtgct aatacttgga tatcttgtgc acggaatccc ttatttttcg ctttttccac
    35161 aattttagag atcaagggtt caatttgata agcatcactt cggaaatagg aacgatcttt
    35221 ttgattcttt gtgaaatctt ttggcagacg tccttctttg atctcatggg caagaggaat
    35281 aatactagaa ccatcacctt gccgataaat ctgattgagt tccattttgg gaatttgttc
    35341 gatttccaaa agatcatgga gtacttgacc aggaccgaca gagggaagct gatctttgtc
    35401 tcctactaag ataacttgca tattcgtcgg aatcgctttg aataatgtgt tagccagcca
    35461 tgtatcgacc atagacatct catcaacaat caaaagtccg ccttctaatt ctttcgtact
    35521 gactgaaggt gttttttccc gtccattcag ccccagaaga cgatggatcg tactggcagg
    35581 cagacctgtc gtttcgttca ttcgtttagc tgctcggcca gttggtgctg caagtagaat
    35641 aggaaaaatt tcttgggtat attttgaagg atccaattcc agtccgttca actctgcaaa
    35701 aagattgacg attccgttga tcacagtagt tttccccgta cctggtcctc ctgttaaaat
    35761 aaatagaggg gaacggattg cttcagcgat agcttgttct tgcgaaggtc catactgtat
    35821 gccgaatatt ttttcgatac gccgaatatt cttctgcact tctttttcag gataatggat
    35881 ttctttttta cgactgagaa ggcgctggat cgaattaccg atcccccatt cagaaaagta
    35941 caaactgttt tcgtaaagat tagtctcttc ttgttgtatc tttccttctt caacaagttg
    36001 gataattaca gtagcaacct gatcaggctg gatttcaatt gggcgacttg tttctaaaat
    36061 acggatcgtt tgctgaagca atgtctccgc cggtacgtat gtatctcctg tttgaactgc
    36121 atgctgaaaa atctgatgca agatagctgc tcgaattctt ctcggtgaat ctgctgcaat
    36181 accgatttgt tcggctagat tatctgcacg tttgaacccg attccttcta tgtcttcgac
    36241 tagctggtac ggattttctt cgatgatatc caaagactcg ttcttatagg cctggtagat
    36301 actgaaagct aactgactac cgaacccgta acgattcagt cccatgatca cttgatccat
    36361 cccgtggttt agccgaatcg tctcagcaag catttctctt tttggcttcg tcaaaccgga
    36421 gatttgtttt aataccgaag gatcagcaat gattttttcg atagcatctt ctcccagcaa
    36481 ttctacaatt ttttctgctg ttttcttccc gattcctggg aatttttcgc tggaaagata
    36541 cgctacgatc ccatttgcag ttgttggctt ttcttgctca tatctttcga gtttaagctg
    36601 ccttccgtat ttaggatgat ccactaattc tccataaaaa cgataagtct cattttcttg
    36661 aatatcgccg aaacttccgg tgactacaat ctctttttca cggtaatcag ctgtggtttc
    36721 actgatgtgt accaaaagca ctttgtaaaa attactagga ttttgaaaaa aaatcgcggc
    36781 aactgtacca acgaaataag gcatttcttc tggctgcatc ggaagccgcc tccttcttct
    36841 atagtaaagg ttctggtgct gtttcagttc ctaaaaaatc tgtttggttc catactttca
    36901 gttcatatgg caataatctg caaggctctt cagtctctaa aatcgtcaaa ctactattga
    36961 acaatccacc catttcacgc aactcactta gctcttttcc tgtcatccat tgaatggctg
    37021 cagttaaaga agctccatgt cctacgaata aaagtgggcc atctttatgt tgggcaaccg
    37081 catctgctac aacagtttcg atacgtgtga ttgccgattc aatcgtttct ccgtgaaaag
    37141 gagaaggatc atacagatct aaatgattac gcaaattcgg cagattatcc ggatatttct
    37201 ggtacatttc atcgatcgac tgaccttcta aagtacccag ccctagttct cttagcttat
    37261 ctgtataaaa aatctctacc ggacgagcaa gctctttgtt gataccttct gctgtatcac
    37321 gtgctcgctt tgctgtactg gaataaattg ctttaaaagg aacatatttt acctttttcc
    37381 caaatgcttt gatttcttcg taactttgtg gcaaaagcgg agaatcgcca ttgctccctt
    37441 gaaaacgtct ttcctgattc cattgtgtct tcccatgtcg ggtaaaatat agttccatac
    37501 gcagcaaact cctgtctttc ttttatttta ctctataata aactgtctaa tccagcttgt
    37561 cattttcata gtgatgcgtc aattctaaat tagggaatcc ttgctggcga agtgcttcat
    37621 acactatcat tgcagctgta ttcgataaat tcaatgagcg gacatgctca tcattcatag
    37681 ggatgcgtaa acacttttct tcgttttcac gcataaatgt ttctggcaaa ccggttgttt
    37741 cttttccaaa taaaaagaag tgctcttctc catcagcaaa attttcgtca ctatatactt
    37801 tatgggcaaa ttttgagatc aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt
    37861 ctaaattact gtggtaagta atatttacat cattccaata atccaaccct gcccgtttta
    37921 gatgtttgtc atcagttgaa aaacctagtg gctcgattaa atgaagcgga gtattcgtgg
    37981 ctgcacacgt acgggcaata tttccagtgt ttgcagggat taatggttca aataaaacga
    38041 tatgattcat caggatcatt cctctcatat ttatgttcgt ttctattttc tgacaatttg
    38101 atttttgcat aaaaaaaaac gtatcaactc ggtgtcagtc actgcgagtc gttacgttag
    38161 tgaagctctt ctcactaact tttatcagaa caggaacctg ataaaaacca atgaaaaaac
    38221 aacttgttgc taatataaca ccctcaactt ttttttgcaa cctctactca ttaaaaactg
    38281 ctagaaaaac gttttctctt agttcctttt aataattcta tgtcaattgt aatcttcttc
    38341 aaaggattct tatataagtc tgctttgatt cgctcttcta cctgccattc taaaggtgac
    38401 atggccaaaa gatctttttg attttcctct ggtatctcaa attcataggt tacacgctga
    38461 cgttcagtta atttcattgc ttcagcaaac ttggctacta cacgttcatt ggaataactc
    38521 tgtttttcag gttgattcgg ataaaaagcc tgacgtagtt cctttaaata attttcttgt
    38581 ggcaccacct tcaacacttc tccatcttct gagagtacac gttggaattc ttggtaatga
    38641 gaaggcgaaa agatatttaa aaccgcgttg aatgtttgat cagcaaacgg caaattggtc
    38701 aaatcagcta cacaccaaaa tgcttcggca gattgttctg tagctgcgta gactccttct
    38761 tttgaaatat caaatccaac agcattttga atctttttca attttgaaag ttcattcaag
    38821 aagctacctt ctccgcagcc aacatctaat agcgagactt cttcaggtaa atgctgcgca
    38881 atagtctcaa taacgggctg atacatgcca gattggatca ttctacgcct tggaacgaac
    38941 attccttgat cgtattctgt tttaaccgca tgggataaaa aataaagtgt tccttttttg
    39001 gaaaggtcaa aacggtgatt atttttgcat atcaagccat tatcagactt ttttacttct
    39061 tcttggcaaa ccggacagcg gaacagctga tgattttctg ccaaaaactg gcgtcctcgg
    39121 tcaatttttt taacatagtt ccccctcatt tctgttttat cttaccgtta gatgctcact
    39181 ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata aaggagaaga
    39241 ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag caattgctag
    39301 aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac
    39361 tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg taatgacgat
    39421 tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa tcatgcatac
    39481 tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt gtcttactcc
    39541 ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag aagggttgca
    39601 gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg aagcaatcga
    39661 ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac
    39721 tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg gccgcttaat
    39781 cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta aagaattgaa
    39841 cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa
    39901 aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta cataaaatag
    39961 cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc gctcaaagtc
    40021 gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat tagcatcggg
    40081 attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat ccctatgaca
    40141 aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt gatccccgta
    40201 acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac aaatccatga
    40261 tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg ggttccagca
    40321 ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc ttcacttacc
    40381 aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc
    40441 agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc ttccacattg
    40501 accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc gatgatatca
    40561 tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag agctaagaag
    40621 tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg aactgaaagc
    40681 actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc
    40741 agagaaataa cccctactag ctgaccttct tgattaacga catagatgta atagattgtt
    40801 tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt ttgattggct
    40861 acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc gtaatggaga
    40921 agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt agcaatttgc
    40981 tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc agaaagcata
    41041 tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc gttgtcttct
    41101 tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata gatcagctgc
    41161 ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg catctctaaa
    41221 aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa ctgggcaaat
    41281 ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc
    41341 gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc aactcttctg
    41401 taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt attcccagat
    41461 ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa
    41521 ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg atttctgtta
    41581 gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag accattcgct
    41641 tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt gccgataaat
    41701 ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt tcacgaagtt
    41761 gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa ccagatgtat
    41821 cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt tttttataat
    41881 aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga atagacgaaa
    41941 cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata tcgatcggtg
    42001 tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg gttaccttat
    42061 catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg atttgaccgc
    42121 cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt aaaaaatatt
    42181 ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca atcctcacta
    42241 ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc aaaatggatc
    42301 cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac attcaattga
    42361 tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca ttcatcatgg
    42421 gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga agcaatctct
    42481 gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact tttattatat
    42541 gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact ttcaaacagc
    42601 tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat cgttgattca
    42661 ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg aacatccagc
    42721 aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag ttcttccagc
    42781 tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc
    42841 acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg
    42901 actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt
    42961 gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac gatcgctact
    43021 ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat agtagacatc
    43081 atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt cttcacggat
    43141 actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa gtcgttcact
    43201 catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa tcgttgccca
    43261 gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct caccgctgat
    43321 cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg cttttttatt
    43381 tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga tgatctcaac
    43441 aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa tatcagacat
    43501 ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta caatactatc
    43561 gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt gttcacggaa
    43621 ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt catatggtgc
    43681 taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa cattcattgt
    43741 ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg accaaaaaaa
    43801 gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga
    43861 atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact atcaattaac
    43921 agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg aacagctgaa
    43981 gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat atacattaaa
    44041 aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag aagaagcaga
    44101 agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa aactttctga
    44161 tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa aacgggctga
    44221 attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca atctacacga
    44281 ttttgaactg gaactagagg taaggggatg catctgtggg aaaaattgct tttataaatt
    44341 ttttaaaacg tttttcttta ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg
    44401 aagctaaaaa ttagaaacct ttcattttca caaggcactt gagttcattt ttttactttt
    44461 ttctgttaag ttataggatg tgaaagaatc tagtcactag ttgtatagaa tctattttgg
    44521 gggagattca tatgatcgaa atctatttat ttgttaatcc tttaggggaa tactgtttcg
    44581 aaatggaaca acagttatta caatttatcg aagaagagta cgggacacca tcaaaagaaa
    44641 agatgcaatt tcgctttctt cctttggtca atttgcagac aattggggat gtcatgcaac
    44701 aaaaaggaat ttctcagaac aatcttgagg aacgtaatca cttattctca atgacttatt
    44761 ctgcagcatt ggactgcaaa gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt
    44821 tgatcaaatt gcaagaagca gtcggctgta atgacatacc atattctcaa gaacttgctg
    44881 aatccatatt tgaagaaatc ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg
    44941 acttcgttaa agaagtattc ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa
    45001 aacacccttc tgcagtcgtt tacaattatg cctgcgaccg tgattttggt gttttagtag
    45061 aagtaaaagg tacaagtagc ctttggagaa tcacagagct gtgtaaaaca acagataact
    45121 catatcaagt ttttaatggt gaacatttac atacaaatga ttctcagttc cattcaagaa
    45181 ctagccattt acacttgctt tctaattaaa aaaatacgtc gactccctta caagagagcc
    45241 gacgtatttt tttcgtttct attcgagaaa atcaaaaata gaaagcctta taaacgatcg
    45301 atcaattctt ctaattcatt tagacgttgt tcaaacatcg acattgcatc ttcaatatat
    45361 gcagcctgtg tcatatcaac tcctgctttt ttcattactt ccacaggata atcgctgtta
    45421 cctgctttta ggtaagccaa gtagttctct agtgcctctg gttcctggtt tagtattttc
    45481 ttcgccagtg ctgaagcagc agaaaagcca gtcgagtatt ggaaaacata gtaattataa
    45541 taaaaatgcg gtatccgtga ccattcgaat ttaatttctg ggtcttcttc gactgctgga
    45601 ccataatatt ttgcattcaa tttaccataa ctatcactta ggtattcact agtcaatggc
    45661 acaccttttt catcttccgt atgcatgaaa tgctcgaatt cagcgaattg tgtctggcgg
    45721 aaaactgttc ctttaaaccc atccaagtaa tggttgagta cataagcccg tacacgaggg
    45781 tctttttctg tttccaataa atactccgtt aggatatttt cattcgttgt cgaagcaatt
    45841 tctgctaaaa agatggagta gtcgccgtac acataaggct ggttcgaacg agtgaaataa
    45901 ctatgaacac tatgtcccat ttcgtggaca agcgtaaata gctgatccag cgtatcatgc
    45961 caattcaata aaatatatgg atttgtgtca tagcttcccg aagaataagc accgcttcgt
    46021 ttccctttat tttcgacaac atcgatccaa cgttcagaga atgctttttc tacgatggcc
    46081 atgtattctt cacccattgg tttcagtgct tctaaagctt tttcttttgc ttcttcgtac
    46141 gtaaaggtaa ttggagcttc ccctaagacc ggtgtataaa gatcatacat gtgcagtttt
    46201 tccacttcta ataaacgttt ccgtaattcc atgtatcgat gtaacaaagg caaatgcttg
    46261 tttaccacgt ctaccaaagt atcgtataca ctttcaggaa tatgattatt gctcaaagac
    46321 gcttctctgg cagagctgta attacggact ttcgctttaa aattatgtcc ttttatatgt
    46381 gtgcctaaag tagaagcaaa tgtatttcta aattgttcgt aaacactgta caatccctta
    46441 aatgctgctt cgcgcaccct tcgatccgtg ctttctagca actgaccata cacgccatga
    46501 gataattgga ctatttcacc attttctcct tcaatcgttg gaaaaactag atctgcatta
    46561 ttcaaaacag caaatgtatc acttgaagca tcaaagattt cacctgctcc agcaagaaga
    46621 gattcctgct cagcagataa gacatgggct cgattatcta ctatttgctg gatataatgg
    46681 cgatagactt ccaattttgg ttcttctttg aaatattgcc aaatctggtc atctgacaat
    46741 tgcaatattt ctggttcaaa ccaggaaacg gcttcactaa ctttcgaaaa cagactgctt
    46801 gctcttgcat aaagcgcttg gtaatctgta tttccagtgt cttgatcgtt tttaaggtgc
    46861 gcatatacat aaatgacttc agtttggcga taaaccctca atacgaattc aatcgcattt
    46921 aaaaattgag aagcgccttg atcaagtgtt cctttgtgtt tttcagattg ttttaactct
    46981 tctgataatt ccaaatattt ctcatcaaac tcttggtcgc ttgaaaagat cttggtcaag
    47041 tcccaagtta aattttcagg caattcttct cgttttggca actgctttac ttccatgaaa
    47101 tccactcctt ttcttccctt agctctatct atcaaaatag atcatagtct aacatccaca
    47161 tgtcacagca tctaactttt ctactcattc atgtgtctgc ttccatcaat tttagcataa
    47221 ctaaatcttg ttttacatag aaaaccgatt cctagcctat ttttatactt actatttttt
    47281 caaagaagac aagtgtatgc attcgttaaa aaactgctga tagaccatct cttcatcaat
    47341 cataggaaaa agccattccc ttttatgttc ttttatttgg caaagccatg agttgaaatc
    47401 aggaactttc tggttttcca aggcttcttc gtacaacatt cgataaagaa aaacctgttc
    47461 ttgtaaataa aagaaaaacc gacttttttg atagatccac gatgatagat gcaaaagatg
    47521 tttatgacgc aagtagcatt gttcttgtat ggatactgta ttcttttgct tgtgaataag
    47581 ttggcgtgtc agccatttct tcccatcttc actcactttt tctatttttt tcatttggtt
    47641 cattttaatt tcgttactag tgaatatttc tttaaggtcc ctactagaaa aagtccagca
    47701 gctactgcaa aaggaaatat gccctgatac agtctctgta atatgatgat acaaataaag
    47761 cttttgtttc ttgagatcag ctttccagca atgcactcct ctttttctat tatagtagca
    47821 gcaacttttt tctatcaatg agaattgcga ttgtccaaga aaagaattac caagtatcca
    47881 ccatacttgg taactgttat tcaaataatt ttgggttctt tcaataaatc tctggtgaga
    47941 cagtctagaa cattggattt ctattgctag attcgccaac aaaatatccg gcctttgccg
    48001 caacctcggc aaataggctt ccaaaaatac tggttctact gactgtccca gccaatccat
    48061 gaagcattcc tttaactgta agtgctcttt tgattctctt tcactaaaga ccgaacagtt
    48121 atctgatttt tgatgggcaa aatgagcaca ttttttcttt ccctttttca aaatcactct
    48181 ttttcgacag ccacgacaat aatatttctt tcctttttct gcatgtttag caaaacagca
    48241 cttattatat tcattcattg caagcagcat ttttctccct ctgaacttat ttacgtctaa
    48301 gaatttattt acacgaaaaa agagggcgtg aaaaagctat tctgcatcaa gtaataagaa
    48361 cagctaaaca aaaatagttc ccaatatttt tcgtttggtt gggcttgtta ccgcagatgc
    48421 aagcttttga acacatttat acgaaaaaaa gactgcgaca caatttttgt cacagtctct
    48481 tactataatg ctgatttata caaaataaaa tttcgttcgc tcgatagcat tacgcgccat
    48541 cagacattgt ccatattcat tcaatgtttc tggtgtacga tcgctctttt cagcaaactc
    48601 caaaattcga gcaatctgat tctctacatc cgcttggttg agattttcca tccagaagtg
    48661 aacgactagg taatagcgct cttcgaataa atacaaatca gtccaagcag actctagttc
    48721 gacatctgtt gcaagttgga tcacttgttc aaaatcttca aaagcaaaag ttgcttgtct
    48781 ttcctgttct tctagggcgc ttccagataa ttcttcctcc ggatcgctgc caataatctg
    48841 ctgacgaaga aagtcactca catcttctga atcaacagta gaatcttctg attgagaaat
    48901 ttcttcatcc ggaagatttt tactgataaa cagttctaat ccgtctcctt ttggcaaaac
    48961 ttggaaagtg acagcttcgc ttcctcggaa ctcctcttcg atatcaactt cttccaaaat
    49021 actataaaag aaattttcga tttctttgtg attgccaagt aaatccaaaa atgaaacgcc
    49081 ccgttcctct aaatcttcat gaccaattag tacacggatc gtattttcat taatatgttc
    49141 catttccatt ttgctacacc tcactttgcc acaatgttta atatagattc attgtagcga
    49201 aatcagctta aatgtaaaga aaaaaaatct cgctgttaag cgaatttaat aaaaccagcg
    49261 gatttttcaa cctttcaagc tatcttattt aactataaca tttttggcag acgaatacta
    49321 tatagccgac ttttcagcta attttccctc tctatttttc gtgcaatcat catcttctac
    49381 taccttttta tacatctaat caagaatact ttttgtaaaa acaaaaaaac ttggaaaaac
    49441 tatctttgta atagtctt
//
LOCUS       DACCWK010000011        48746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCWK010000011 DACCWK010000000
VERSION     DACCWK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.011"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(86..997)
                     /locus_tag="IVW65_000559"
     CDS             complement(86..997)
                     /locus_tag="IVW65_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7899482.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1454..1882)
                     /locus_tag="IVW65_000560"
     CDS             complement(1454..1882)
                     /locus_tag="IVW65_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899483.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1910..2548)
                     /locus_tag="IVW65_000561"
     CDS             complement(1910..2548)
                     /locus_tag="IVW65_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899484.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2552..4588)
                     /locus_tag="IVW65_000562"
     CDS             complement(2552..4588)
                     /locus_tag="IVW65_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7899485.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4714..5379
                     /locus_tag="IVW65_000563"
     CDS             4714..5379
                     /locus_tag="IVW65_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7899486.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(7314..10463)
                     /locus_tag="IVW65_000564"
     CDS             complement(7314..10463)
                     /locus_tag="IVW65_000564"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP7899487.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(10476..11675)
                     /locus_tag="IVW65_000565"
     CDS             complement(10476..11675)
                     /locus_tag="IVW65_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7899488.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(11665..13260)
                     /locus_tag="IVW65_000566"
     CDS             complement(11665..13260)
                     /locus_tag="IVW65_000566"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7899489.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(13844..14551)
                     /locus_tag="IVW65_000567"
     CDS             complement(13844..14551)
                     /locus_tag="IVW65_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899490.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(14616..15452)
                     /locus_tag="IVW65_000568"
     CDS             complement(14616..15452)
                     /locus_tag="IVW65_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7899491.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(15470..16879)
                     /locus_tag="IVW65_000569"
     CDS             complement(15470..16879)
                     /locus_tag="IVW65_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7899492.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(16893..18716)
                     /locus_tag="IVW65_000570"
     CDS             complement(16893..18716)
                     /locus_tag="IVW65_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7899493.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(19559..19885)
                     /locus_tag="IVW65_000571"
     CDS             complement(19559..19885)
                     /locus_tag="IVW65_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899494.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(19931..20293)
                     /locus_tag="IVW65_000572"
     CDS             complement(19931..20293)
                     /locus_tag="IVW65_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7899495.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(20330..20545)
                     /locus_tag="IVW65_000573"
     CDS             complement(20330..20545)
                     /locus_tag="IVW65_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7899496.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            20672..21007
                     /locus_tag="IVW65_000574"
     CDS             20672..21007
                     /locus_tag="IVW65_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7899497.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(21083..23080)
                     /locus_tag="IVW65_000575"
     CDS             complement(21083..23080)
                     /locus_tag="IVW65_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899498.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(23178..23453)
                     /locus_tag="IVW65_000576"
     CDS             complement(23178..23453)
                     /locus_tag="IVW65_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7899499.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            23685..24419
                     /locus_tag="IVW65_000577"
     CDS             23685..24419
                     /locus_tag="IVW65_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7899500.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            24458..25600
                     /locus_tag="IVW65_000578"
     CDS             24458..25600
                     /locus_tag="IVW65_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7899501.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(25695..27086)
                     /gene="sufB"
                     /locus_tag="IVW65_000579"
     CDS             complement(25695..27086)
                     /gene="sufB"
                     /locus_tag="IVW65_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7899502.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(27091..27561)
                     /locus_tag="IVW65_000580"
     CDS             complement(27091..27561)
                     /locus_tag="IVW65_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7899503.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(27548..28783)
                     /locus_tag="IVW65_000581"
     CDS             complement(27548..28783)
                     /locus_tag="IVW65_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7899504.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(28780..30066)
                     /gene="sufD"
                     /locus_tag="IVW65_000582"
     CDS             complement(28780..30066)
                     /gene="sufD"
                     /locus_tag="IVW65_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7899505.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(30079..30852)
                     /gene="sufC"
                     /locus_tag="IVW65_000583"
     CDS             complement(30079..30852)
                     /gene="sufC"
                     /locus_tag="IVW65_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7899506.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(31049..31897)
                     /locus_tag="IVW65_000584"
     CDS             complement(31049..31897)
                     /locus_tag="IVW65_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7899507.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(31921..32607)
                     /locus_tag="IVW65_000585"
     CDS             complement(31921..32607)
                     /locus_tag="IVW65_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899508.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(32600..33634)
                     /locus_tag="IVW65_000586"
     CDS             complement(32600..33634)
                     /locus_tag="IVW65_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7899509.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(34029..34367)
                     /locus_tag="IVW65_000587"
     CDS             complement(34029..34367)
                     /locus_tag="IVW65_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7899510.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(34369..34725)
                     /locus_tag="IVW65_000588"
     CDS             complement(34369..34725)
                     /locus_tag="IVW65_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7899511.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(34731..35918)
                     /locus_tag="IVW65_000589"
     CDS             complement(34731..35918)
                     /locus_tag="IVW65_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7899512.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(36109..36954)
                     /locus_tag="IVW65_000590"
     CDS             complement(36109..36954)
                     /locus_tag="IVW65_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7899513.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            37188..38153
                     /locus_tag="IVW65_000591"
     CDS             37188..38153
                     /locus_tag="IVW65_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7899514.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            38530..39573
                     /locus_tag="IVW65_000592"
     CDS             38530..39573
                     /locus_tag="IVW65_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7899515.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(39635..40099)
                     /locus_tag="IVW65_000593"
     CDS             complement(39635..40099)
                     /locus_tag="IVW65_000593"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7899516.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            40345..41292
                     /locus_tag="IVW65_000594"
     CDS             40345..41292
                     /locus_tag="IVW65_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7899517.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(41503..43575)
                     /locus_tag="IVW65_000595"
     CDS             complement(41503..43575)
                     /locus_tag="IVW65_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7899518.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(43508..43885)
                     /locus_tag="IVW65_000596"
     CDS             complement(43508..43885)
                     /locus_tag="IVW65_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7899519.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(44014..44574)
                     /locus_tag="IVW65_000597"
     CDS             complement(44014..44574)
                     /locus_tag="IVW65_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7899520.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            44724..46016
                     /locus_tag="IVW65_000598"
     CDS             44724..46016
                     /locus_tag="IVW65_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7899521.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(46076..47194)
                     /locus_tag="IVW65_000599"
     CDS             complement(46076..47194)
                     /locus_tag="IVW65_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7899522.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(47208..48629)
                     /gene="arcD"
                     /locus_tag="IVW65_000600"
     CDS             complement(47208..48629)
                     /gene="arcD"
                     /locus_tag="IVW65_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7899523.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
BASE COUNT        14153 a        10007 c         8278 g        16308 t
ORIGIN      
        1 aactactgtt gatttagttc taataaaaag atttagatga acagttttat tgatttaaat
       61 agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa tctgaagtga
      121 atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg tctttcaata
      181 cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct gttgcaattt
      241 ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt ttcaccattt
      301 taaccacata atattccgta gtataagttg aagcattggt agaagtgatc acatcagaaa
      361 cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc gttgtagtag
      421 tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt gacttgtctt
      481 tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct tttgcatcat
      541 cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat tcccaagccg
      601 tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga aaggctagac
      661 tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc aattgtgaat
      721 caaatgaatc acccaattgt ttctttgttt gatcatattg agaatcgatt tctttggttg
      781 atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt tgcacagcgg
      841 aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg attgtaccgc
      901 ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat aagccagtgc
      961 ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc tacttttcgt
     1021 tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga acacctttac
     1081 taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa gttcgctcag
     1141 cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg ttaccgtttt
     1201 tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa tgttatcgat
     1261 tttgtttttc ttcttccgct gctgggatat tcaaatcaga cacttttatg ttcgattttc
     1321 cagacatttt tttgttgttt ttattatgat atatttttat acaactaaaa atgaatatat
     1381 taaaactacc aaaaataact tttaacgctg tttcattata tgttttttct attttatata
     1441 aacttttgga tacttaatac caaccggttg gattgtacca acatgagaat gtgtcagttc
     1501 tatttcctgt cgcacttgca taggcccata ctccttttgt tgcatttgct gatcgaatag
     1561 agtttccaac tttcgctttt gcaccgtgac gattatagac acgactatag tggttagaat
     1621 gacctgtttt tctcgtacaa gttgtatcac tataataaac ccatcttcca gagctatgat
     1681 taattgctgt tctagaaatt tcccggtcta ctggtaaatt tgacttaata atattacctt
     1741 tagcatcaaa atgaatctct cctggcttta aagatttgta atagatttca ccgtgaactg
     1801 aatttgcttc cacttcagat gacatagcgt ttcctccaac aaaacctatc cctagtgcac
     1861 aaataataac tgcttttttc ataaaacgac atcctttcct ttttgatttt taaagcatga
     1921 tcttcttgtt tcctattttg gtcagatatg gatcatgcgt gactacgata actaacgtac
     1981 cttgggaagc ttgttgcgat aagatttcaa ctactttatc cttatttctt ttatccaatg
     2041 acccagtcgg ttcatcggct aaaatgattt tgctattctt caataagact ctcgcacagg
     2101 cgattctttg cttctcacca ccagataatg tgtagatagg cgtacttagc ttcttgttta
     2161 tatttacttt tttcaatgcc tcatactttg ctgttcgctt atctgaaaca tttaaattcg
     2221 aaaacttcaa tccaatatct aaattaaaat cgattgtttc atattcaatc aacccaaaat
     2281 cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg cttctctctc ccagttaatt
     2341 gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact gaactgatca ttcaatccta
     2401 atatattcaa taacgtactt ttacctgctc cactttctcc aacaacagtc acaagatccc
     2461 cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg atttttttcg
     2521 caccttttat cactatcaac ggctccaatt gttaacctcc attcatcagc aataactgac
     2581 ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg aagctaaaga
     2641 aaaaaatatt aaaaagatat ttaggctctc tttctataag aaaaaggaag attacacttg
     2701 tcgttcccca tttgatgagg ttcatcatga agaaatcttt atacgtttca aagagcgaat
     2761 aaccacttga acgaaagata gcgattttcc gacaattgta gacaaaatat atgcataaca
     2821 tgacatagct caacaagaag aaagaaaaga aagtgagtaa aaatatctgt atcaagtaat
     2881 gaaatgatct gcttaacact ttgacagcta tctcttcagt ctgagtatac ggaaatgata
     2941 actgcaagtt atcacgtaac tgactttctt caatcagctc aaataacctt tctttcgaca
     3001 aagagcctat cttgatcttt aacggtggat agatttctcc gctgagaata tttctttcca
     3061 aatacatact gtttttcaat gtcagtattt caacaaccgg ataatgttcg atccagggga
     3121 tagaaggaat aaatgtataa atgggttgat tgttttttat aaatagaaat ttcggttctt
     3181 tttcactgat ttcttgataa tattctgtta tttgcgccag gtcatttttt aaatctgtat
     3241 gtctatatcg ttcagggatc aatataacag ggtgcgtttc tttttcggtt atttggatca
     3301 accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt atttttatcc
     3361 ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact aatatacttc
     3421 cgtttttatt caaagtttca tataattcct gatctgctgc tgatgcaaaa cgattggaat
     3481 aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga tagtcctcac
     3541 cagcgatttc ttttggtggt tcatgggaaa cagctaactg tgaagaagaa aaaacgatag
     3601 ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca aatgtaaaaa
     3661 gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca actaataaaa
     3721 gagaaatcaa agagatacca tatattccgc tgaacaagaa aacgtacatc tggaacaaat
     3781 aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct gcaattatga
     3841 atgtaataat gattttcttt ttccccaata aacaccacaa tacttgatgt aatgaaaagt
     3901 cattattcaa caataaactg atttctttat tcatcaataa gaaaaaggat gataacaaaa
     3961 ggaataaaag aatcaatcca atagttaaat acactcgata attatatacg tctgtatttg
     4021 aaataaccga atacgaatag ctattttcaa agtcttcaaa aatattagtg gcataatcag
     4081 tgtgaaatgc ttgattgaat cgatcagtga atagtgaaat gacccgttca taggcttctt
     4141 tttctctcgt catcaagaaa aattcacctt caaaatcttt tattttgatc gattcttcaa
     4201 tggacgttac gcgaaaggag gtattagaat agggtacctt ctcgtgagaa gatttgacat
     4261 ccccttgata atacaatgtc atttcaagct ttggctgtat cttgaagttc caagatgtat
     4321 ctacttcatt ggattcattt actgtttttt tcatataaga aagctcagtt tctgatgaga
     4381 cattttcaaa taccatgatt agctgctcat attcttcttg cccaataaaa tgttgtggca
     4441 atcgaatcgg agtataagta tctgtttgat ggaaaaattc atttgtccga tagagtacct
     4501 gtttatttat actaaccaag gcaaaaccac tcatgatacc ggtcactatg attgtgagta
     4561 aaactgtgaa cttttttttg attatcacca ctctccctta cttaaatgat tctttgataa
     4621 aatcataccg tttttcatca aaaaaacgta tctatcaagt gtataggtca tgtagaataa
     4681 atctatatac tgtagaatcg aaaggagaag aagatgaaca ccggagaaaa aatacgaact
     4741 taccgcagaa attgtaagct tacgcaaaaa gaattaggcg aaaaaattgg tgtttccgat
     4801 aaaacgattt ctagttggga aaattctcgt acaatgccag actttgaaat gctgtcactt
     4861 ttacatcata ctttaggtct gccgattgat tttccttcta ttgaatcagc taccgaatca
     4921 atagaaccaa cattgccaga tgaacctctt gttaacaaga aatcttattc atcaaaacaa
     4981 cccttctcac tcaaaaagac actatctttc tttctgctcg tcttttgttt ggtctatctt
     5041 tttcttatca attatcaata caaaaatgaa acgatcgatc gattcaatcc ttttttgaaa
     5101 ccacaaatcg gctataccac actccctgta ggatacgtgg acaattccat cgaacgttgg
     5161 gtatgtaatg atatctttgg ccatggtaca tggctgtctt ttcacgctgg agaggtagaa
     5221 aaagagaaaa accatgctgt aatcaaacac aaaggaaaac acgtcatgaa cgttcgcctg
     5281 ctcacttggg aagccattcc ctcagattac caagaaatcc tcagccaaga ctatgacgac
     5341 gccttaaaaa gaagccctca ggagccagat aggcattaag taggagatga acgtagagat
     5401 agaaaaaatc aatgcacatg atcttatcca aaaaacaggg gtacaaatta attgaaaaag
     5461 ccttgagatg aaagttccta aataaaaaag ttcagaatga taggtatttc tggcttatag
     5521 tataatattt acgccacagc ctcccaatga ttctttttgg aatcataatc aaggatggac
     5581 attcacaaaa atattttgct cattcgatcc gaatagatta acgaccaatc tagtaagtaa
     5641 tatagattgg tacgtagtca agctgcacat aatttagatc aattttagtt cttttttata
     5701 tttctatgat ctgctcattt acttttatat gatggcttac agtaacataa ataaatatga
     5761 taatcgctgg tagtttatcc aagagtactc aataaaatac aaaatggatt gttcaagcgc
     5821 ctatcataat gataaaatga aacaaataaa cgatacaagg aggaagagac tttgagtaat
     5881 ggcagaacaa ttttttcatt gtaaaaattg ttcagttgtt aatctatggg atttcggtag
     5941 catgggcatt tttttataga ggaatcacac caacctggcc gttttatctc ttgttagtgg
     6001 gaatattagt aggaaatttt gcccctcttc actatcgtac gaactttttt ccaactgatt
     6061 ctggcctttt tcttaaaaca aagacgcgaa aattagaaaa cattattgaa atgacaatta
     6121 cctgtataat cattttctta gttatattcc taaacgtctt ctaataagaa tctcttaaaa
     6181 ttagcttgtt acagttagtt acctagttta tataacacag ttatcttatc ttccttatca
     6241 acatcttttg ccaactcttt gtcttaaatt cgaaacatat aagcagagct acgaacacca
     6301 agactgtttc ggctctgttt tttgcatcaa attcactctt atatagctga taatctcggc
     6361 tctttaaaag aaattatttt cgcttcattt tattataaaa aggctaggaa ccttgatgtt
     6421 atcaggttcc tagcctttat ttaagtatta ccccacagac ccttccatat agatttttaa
     6481 attacacact agtttaaact aaaacaaaca aggaaaaact taccatttaa tcaaaaaatt
     6541 agctcatatg aatatattag aatgtaaatt agactggtca aaatctggtc aaaaaagagg
     6601 acgaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
     6661 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
     6721 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
     6781 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatacagtcc
     6841 cataggtaaa gtgccacagg actgtatatt tttttatttg agattacctg aaatgattcc
     6901 atttaatttt tatctcattt tttcctacta gttagtatca aatactctcc tattttctca
     6961 ataaactatt ttgggttaca tggtatctga aacagctaaa accttgtcat tatagcaaaa
     7021 aatcacttta tctccgtttc atttcagata catttcactt aagcggattc caaaaaaaca
     7081 aaagaaaaag gaacccctag ttagcctatg ctttttattg gttcctctca aaacacggtc
     7141 atataacaac ggggtggggt aaatgaatga aaaaggtgga ggaaacatta ttcaatagct
     7201 gtcttaaata cttcgttata aaatgtgaac aaataaaaga catctatccc tattttctac
     7261 acttccactt gactaccatc tttgaatcgt acaattttct taactcttct cgtttacctc
     7321 aattcattct caagtggaat caactcatca tctaacattt tacgccatgc tcgcttcatt
     7381 tcttgtgggt atttaaatgg cttcgcttct ggatgtttgg ctttatatcc ttcgtaatcc
     7441 ttactgttga taattgcttt catattagga attggatctt cacctggtag atattgcaga
     7501 ttagaagcat gtaattcttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa
     7561 tcatcactcg cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attactatct
     7621 aaagtcccca catcaaatgc atttaaaatt tgtcggtaaa cttctttgac tccttctgtt
     7681 ttattcaatt tagccaaagc tttttcaatt tcttctcgtg catctgaact attcgcagca
     7741 taagttccta gtaattgatc aatataagtt gaatcaatat catacaactt agtggtattt
     7801 tcactataaa attcgatcgt tgaaaaatct tctccttctt tttctggttc ttcctctatt
     7861 aatgaaccct tcacggtctc gtacacgcca atttgttctt ccaaaagttg aatttgattg
     7921 ttcaaagttg gagatttcac catgtcatca ttgtaatcgt cataagtcac taacgattca
     7981 taagcacgat tcaattcttg aaatgctttg acgtactcga tacggatttc aagtggcgta
     8041 tgttcatcaa tggattccat ggatgaaacc tcttttaact taccatatgc ttgtttgaat
     8101 ctttttttgg attcctgata acttggataa atcaaacctg attcttcttt tgcttttgaa
     8161 tacagttttg tcgcatcctc gacattcttc tccattgtcg ctggcttacg aaacgtcaca
     8221 atcaatcctt ttgtcttatc aggatacgta cgattcgtac gtgaaaaagc ttggatcaat
     8281 cctgcatact ctagattacg atccacaaat agggtttgaa tcgtaggggc gtcaaaacct
     8341 gttaatagac gatccactac aatcgctaaa tcgatttgtt tcccaaattg tttgaattcc
     8401 gctctttttc gtgccaaacg attattgatg tctccgttat aacgctcaat gtctgctaat
     8461 gaccaggaag tatcataata ctgattatat tcctcaataa ttttttgcat ttcttcttgt
     8521 tgggcacttg aatctcggct attttcttct aatgaatagg taatagcgat tcgtggaaaa
     8581 tcaggatcct ccatcgttcg tccttcacga atgagctgat tgggaaactc ttttgttaac
     8641 caatcaggct ctttcgtcat ctctttgatg gcccgataat actttttggc catgtcaatg
     8701 gaactcgtgg ttaagattgc agatttcgtt ggcctgccat ttcgaaaatc aaattttgta
     8761 taggcattat ctggacggaa cattttatga atgacttttt ggatatgttc atctgcttca
     8821 aaaacagctg ggtccaaata actttctttt ttgacaggtt ccatttgatc aatcatacga
     8881 ttgatctgtt ctgatgaata ctctgcatat gtttctactt cttttaattt ccgatagatt
     8941 ttattctcta gtgaagtcgg ctccacggtg ttttcatgct ctacttgaaa tcctaagaca
     9001 gagccatctt ctagcgcatt cttaatggta taggtatgca agacctctcc atactgatca
     9061 tgggttgtac gtgccaattg ccctttggct tgtttccgat tctcaggaaa aatcggtgta
     9121 cctgtaaagc caaaccaagt ggatttcgga aacatttttt tgatttcttc catattttca
     9181 gcacttaacg ctcgatgaca ctcatctact acaaacacga tatgttggcc cattaatttt
     9241 tggaagcgat tggttccttt tttctcttct tgtttctttg catatttcaa cgccgcatct
     9301 agtttttgac gggttgtaat gattacaaca tttgcattgg catctgccaa cagggtttca
     9361 cttaattctt tggcacttcc cgttccaacg atcaagctat tcgctttggc gttcccggaa
     9421 gaaatcccag tattaaattc cgaagcaaat tttgtaaact ctgtggtggt ttgattatct
     9481 aaatctttgc ggtccactaa catgatcgtt cgatcgatcc ccgattttcg tgctaaaagc
     9541 ttagtagata caaaactagt caacgtcttt cctgaaccag tcgcatgcca cacataccct
     9601 gattgatgtt tatttgcggc aataaacaag gcttgaatcg catgtacttg ataaggatgt
     9661 aacaccatga gggttttatt atcttgatct tcactgacga ttgtataatc cgcaatcaaa
     9721 cgatgagcat ccgggatatt taacacttgt ttacaaaact catataagtt ttctactttt
     9781 tcattgtctt tcgttcgcca actaaataaa aacttagggt gcaagtcttt cggtaaggca
     9841 tttgcaaaat accgagtggt ttgttcattg gaaaccacaa acaactgtaa ggtcgaaaag
     9901 atattgttcc gaaacatacc ttcttctgca tattttttga tttggttaaa tgcctgataa
     9961 acaccatctt ttgcagttac ttgtttcagc tcgatttgga caatcggtaa tccattgatt
    10021 aataaggtga cgtcaaaacg tcgatcccgt gcttctatgt tacttccacg ttttgcaatt
    10081 tgatggacca cttcataagt agagattcct ccaccaatgt cttgattgga ataaaggata
    10141 agtgagacag aacccaattg cgaatcttcc cgttcaatcg tgatccgagc catcccattt
    10201 tcacctttta accatttcgc agcatcaaat ggggttttcg ttcgcagcag taattccgtt
    10261 ttgatcgttt caaattcttt atcggtcaaa ggaaaatcat tcagttctgc ttgattattt
    10321 gagataattt tttggcgtaa attgacccaa aggtcttctt ctgattttag atccggacga
    10381 tacgtccact gattatgccc ttctccaagt acacgaatca actgttcttc gacttctgct
    10441 tcctcacggt gactaatttt cattcttctt ccctcctaga caaacatctt ttgtaagaag
    10501 ctttttttcg tttcttttag taaatctaac ttacgttgat gaagagcgat cgtatcatct
    10561 agctgtttga agaaagtacc gattttttgt tgttcatcct ttacaggaac cttaacactg
    10621 atattctcaa tatcagaact gttataagaa ggcaatgccc ctacttggga gtatcttggc
    10681 aaatttatag tttcaaacaa tattttacca aaatagatat cccaagtatt attaaataaa
    10741 taagccatgg tattgttatc tattaagaaa gggcttgtga ccaaacgttt tctattaagc
    10801 attattgctg cgccaacttt agcaaatata atcgctggca catcttttat tggcttccat
    10861 tttttacgct caatttgctc attagataca taatgatttg catttctcat ttcatactca
    10921 ttaccaatgt tattcatgtc ggaaacttta aaaaatggag taccttcaga accaccttgc
    10981 tcggcatcag gaaatccaat tcccgattga gttttaccta attctcccaa cttacgctct
    11041 tcccaatctt ccgtaaaacc aggaaaccgg atttctggca cttttgctcc gttttcaggg
    11101 aacatttttt gtaagaatcc ttttttcgtt tccttcagca aatctaactt acgttgatga
    11161 agagcgatag tgtcgtcgag ttttttgaag aatatgccga ttttttgttg ttcttttaaa
    11221 tcattgggaa tatagaatac tgtatctgaa acttttttcc agtctgaacg aggcattttt
    11281 gttccagatg tatcatttgc agccttttga tatctatcag cttgaataag agaataaata
    11341 aagtccgagt cgctcttctt agctctaaaa acccagaaat ccccaatcgc tacgcctgta
    11401 aaagttgcct tgagccaatt ttttaaatat ggacgtaact ttccatatag aatatcattt
    11461 ggtaaaaatt caatcccttt tctattatca aatttctggc tcacatcttt attcagtcta
    11521 ccttctcctg ccacgatatc ttcaaattca acttttggaa gtctactgga gtttgtactt
    11581 ttattaagtc taacaactaa atcaattaac ttacgctctt cccaatcttc cgtaaatcct
    11641 gggaaacgga tttctggtcg tgtatcattg ctcatgtttg aacacctcca gcgcaccttg
    11701 taaccacatt tcgttttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
    11761 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
    11821 aattggttct tcttcttcga aagtatctac atagcgtggg atatttaagt tatagtcatt
    11881 ttctttgatt tcatcaaagg ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
    11941 gtaggtttcg aggatttttt gaatgttttc ttccgatagt ttattttgat ttttcccttt
    12001 aacaaattca cgactagcat caatgaataa tacatctcga gtttgacgat tcttcttcaa
    12061 cacaatcacc gtcgttggaa tcgatgtccc aaagaacaag ttcgcaggca taccgatcac
    12121 agcataaata ctgccatctt ctagtaattt ttgacggatg accccttctg cagcgccacg
    12181 gaataaaact ccgtgtggta agacgatggc catggtacct gtttccttca aatgatagaa
    12241 cccatgaaga agaaacgcaa aatctgcttt ggattttggc gctaatttcc cataacgatt
    12301 aaaccgagaa tcatctaaga aagtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
    12361 cattactact gcatcaaatg tgtaaggttc atctgttggc cagtccttat tcaacgtatc
    12421 cccattgcgg agattcattt cttcggcgtc gacaccatgt aaaatcaagt tcatttttgc
    12481 tagattgtat gtcgttgtat ttagttcttg cccatgatat ttaacgttat ctgggtgtgt
    12541 taagtagtta cgtacgttta acatcaatga acctgaaccc atcgttggat caaaaacact
    12601 aaagaatcgt cgttcctttt gatcgagcgt tacgatttgc gccatcatat cagaaaccat
    12661 atgaggtgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
    12721 aaactcatac gcatccccga tcacgtcccc atcgtgttct aaaacatcta catcattcaa
    12781 tttcttgatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
    12841 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
    12901 cacaaaggct ttgtttagat cgttcaattg gaacgttgct tgttttgctt gatccgctaa
    12961 gacgttgaaa agatactctg gtgcaattgc atatcccaaa atatcaacga ttgtggcaat
    13021 cagatcttct tttgattctt catctgaaag taattcacga tacaacattg tttgtttgct
    13081 tactgtgtca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaatcg
    13141 atcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
    13201 cattttacta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa
    13261 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcataccc
    13321 ccttttataa aaagcaaaaa aaaaagaaag aaacttattc ttgctaataa actacgcgaa
    13381 aggagtctaa cgttgtttga tataacttag actgatcgtt aacttatttt ttatatttct
    13441 acatagttat ctttagaacc atttcagata catatgaaac aattacatat gattttttca
    13501 taaaaaaaca gtactgtcta gtagtataaa atatacaaat ttcttaaaaa ttttttcact
    13561 aaaatcacta cctgaaacag tctaaaacac ttatatatca acgtttaatg aaactatctc
    13621 cgtttcattt cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc
    13681 agtagcttat cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg
    13741 taaataaatg aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt
    13801 aatcagttac ctgtccgttc ttaaatacca cagaataatc tttttaccca ttttcaagta
    13861 accgaagtgg aatgtcttta ccaacttgct ctgtcactgc attactgata cctgttagat
    13921 tagctaataa atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg
    13981 tgattccttt atcttcattt acaagcttcc aagtaggtgg tatagctgtg tatgtttcga
    14041 tgaatgaatc aattctttca tctatcgtct tcccagtatc tacttctgtc tcattactct
    14101 tagatgaatc gattctttca tccatcgtct gctccgtaac tgcttctgtc tcattattct
    14161 caccggaaac tctcttcaca cccaaactta tatattgtcc atctttagtg tgcatagtta
    14221 aaattcccac gcttttacta ataaattcac tataaccagt tctttcaata gagacttcaa
    14281 tgtatttcac actatcatac tcaaaagaaa aatagacatg taaaagcatt ttgttgttta
    14341 catctacata ataatccttt gactctactt gagaccttcc tatacggaat acagctttac
    14401 tgtctgatat ctctatacca gcatccttat tattaatctg ccaatctcca agtagctctt
    14461 ctggcaagct agtagtttct tgtgaacact ttaccacttc actggtagag gtcgaaacat
    14521 tcacctccgc actgttagaa gaattttcca aggaatcagg agccttagca caaccaacaa
    14581 gtctttatat attgtgcagc cgatattttc ttcattcagt acacccttga aatatgcagt
    14641 gttagataaa gcagttcgtc ctgatttgct gaggcaccca tctcttcttg aacgtactcc
    14701 ttgattttca aggccgcccg atactctttc ggaaaacgct gcttcactga agccagcaaa
    14761 aaatcatcct tctccggcaa ctcttccttc ttaatcattc gggcaataaa aaactgtaaa
    14821 tgcgtcacaa atcttaagta aagcatcgaa tctttatcaa cctttttttc gggaaacaga
    14881 tatcctagca tagccaagat atcctttagt gctcttaccg agagcatggt ttcttccata
    14941 tttgcttgtt cactttgggc atttacaatg tgaaacgcaa tgtttccagc ctcctcttct
    15001 ggcaactgaa cattcatttt tttgttgatc aattggatag catagcagcc gatctcaaat
    15061 tcttttgggt aatacttttg aatttcaatc agcaggcgat tttgaaaaac aatgcctttt
    15121 tgactgcgtt caatggcatg cttcaagtga tcaaccaagg taaaaaatag acttttattc
    15181 agcttaaagt caaacttggc gttggcataa tcgataattc gcttgacgca aataattaga
    15241 tcatccggag cagcctcaaa cagtttaatg taatcctttt ttagagcatc agaaagtgaa
    15301 tctaatttaa atactttttg gattttgtca gccggaacca aatcgccttt ctttagttga
    15361 aatccaatcc ctttccccat caaaattact tctttgcctt gcttatctaa gcaaagaatt
    15421 aaactgttgt ttaacgtttt aattactctc attagattca ccgttctctc taagctaagc
    15481 catttcttgc gattactttt tgataccaaa agaagctttc tttttttatt cgttttaatt
    15541 ctttaagatt ctcatcggtt cggtcaacat atataaaacc ataacgctta ttgaagcctt
    15601 gatgggtact aacaacatca atcgctgacc aaggtaaata accaaaaaca tctacaccat
    15661 ctgttaaagc tgcttgaata gccagaatat gtttggctag ataatcaatt cgatcctggt
    15721 cattaattcg tctatcttcc tcaactatgt cgggtgcccc atagccattt tcagtaatta
    15781 aaataggtaa atcatagcga tcgtacaatt tcctcaatgt taatcgtagt ccagtagggt
    15841 caactaccca gccatatggc gttttatcta cataaggatt ttcagctgcc cggtagacac
    15901 cttcttctcc caacatgatc tgttgatccc ccgctctagc ggaaacatct gatccgtcat
    15961 ttttgctggc agcgatggtg gcagttgaat aataattaat cgcaataaaa tcgggtttac
    16021 cggaggataa aattgcctga tcctctgcct caattttagg atataatcca cgatcattca
    16081 aatagttttc aaacaaataa ttgtattttc cccgaactgc tacatctaag aaagaccagc
    16141 ctctcaatgt ttcccaatta tgggctgcaa ttgcatcttc tggactggag gtagcttgat
    16201 acatagccgt taaattgatt gctggaccga tttttccttt aagattcaac tgatgataca
    16261 agtgaattac tctagcttga gctaataaaa catggtgatt aatttgaaag agttcttttt
    16321 ttgaaggcag ctctccactt ggtggcaaac caatcgctcc aggatgaagg atcatcgtgt
    16381 tttgctcatt aattgtcagc caatagttca ccttgtcacc atactcagta agcaatacct
    16441 gtacatagtc cacaaaataa tcgatagttg ctcgatttaa ccaaccgccc tgctcctcta
    16501 gaaaataagg taaatcgaaa tggtacatgg tgacaatcgg ttcgattccc tgattcaggc
    16561 attcatcaat tacttgatga taaaaagaaa ttcccttttg attggtttta ttattttcaa
    16621 tatccataat tcgcgtccaa gcgatggaaa agcgaaaagc ttttaagcct aattctttaa
    16681 acagctgtat atcttcttta tagtgatggt aaaaatccga ggcaacttca aaattagcta
    16741 ccccttctgt atgctgatac ttgtcaataa tagacggttg tttcccatcc tcctgatagg
    16801 caccctctgc ctggtaggca ctaatggagg caccccataa aaacttctct ggaaatgctg
    16861 aaagctgttt gtattgcaca aaaatcctct ccttaactaa tttgcatcaa tggttcgtgt
    16921 tcctttaagc ttccgcttgc ttcactaatt cctgcgtaat tttcactgtt ggtcactacc
    16981 acaatcactg tatcatcgta gccagcgttt ctaatggcag cgaaatcggc gttgattaat
    17041 aattgtcctg ctgacacctg atctcctgct tttacaaata cttcaaaggg ttcccctttt
    17101 aaattcacgg tgtcgatccc cacatggatc aataattcaa taccttcttt agaacgtaga
    17161 ccaatcgcat gtttagattc taaaatagta atgacttctc cctcaacagg tgaataaatg
    17221 ctgttttctt ctggatgaat ggctacgccc tttcccaata gtcccttaga aaagacagca
    17281 tcgttcactt cagaaatagg acacacggtt ccttttaccg gattttcaac aatgactgct
    17341 tcggctattg ccgtttgctc tgattcatca gaagtatcgt caaatccaat tagataagca
    17401 atgataaagg ttgcaacaaa ggcgattaca cacgtaataa ttgcgtgaac aatattcatt
    17461 ggattttctc caataaaggc tggtaaggct gctaagccag gggaaacaaa ggcatatctg
    17521 actaaaccgc tcaagccagc atacaaacca gcaattgcac caccaatcat ggaagcaact
    17581 aatggttttt tcagcttcaa ggttactcca tagagtgaag gctcagtaat tcccattaaa
    17641 gcagtgatcc cagtggacac agctagctgc ttcaactctt tgtttttagt tttcgcggct
    17701 acagcaaagg tagcacctga ttgagcaata ttagaagcca gcattcctgg cccattgatc
    17761 atttcagaac ccgtactgct caattgcata gtagcgattg gcgtcattgc ccaagctgta
    17821 cccgtaacca ccaaaaatgg ctgcaaacca cccatcaaaa atggaattaa ccaagctgct
    17881 ttgccgttaa tccattcagc ccccattgct ataagatcgt tcagcaacgt tccaaacggt
    17941 ccaatgacaa ccaacgctaa aggccccata attaaaaata ccagcatcgg cttagtaaag
    18001 aatttaataa acgaaggcga aattcgatcc gcaaagggct ctacatagga catgatccaa
    18061 acagtaaaga taatcggtaa aactgatccc gcataatctg cagctaatac cggaattcct
    18121 agaaaagaaa cggcatcgcc agtagaaaaa agctgtgaaa tatttggatg aagcaaaatg
    18181 ccagcaacag taatcgccaa aattgggttg acatcaaact ttatggctgc cccataggct
    18241 aatagtactg gcataaaata aaaggctgca tcagcaatag tattcaaaat gatgtaagtt
    18301 tgactactgt ctgatagtac accagtcaaa gtcaagacgg ccaaaatggc ttttaccatc
    18361 ccggcacctg ttatagctgg aatcatcggt gtaaaggtgg tagaaatgac actaatcaat
    18421 cttgaaataa tactttcttg attgtctgaa gaggggccct ttttaattga agttcccact
    18481 tccttttgaa tttccttgaa ggctgtttgg acttcattgc caatcaccac ttggtattgt
    18541 ccaccgctat ccaccacact aattacacca agtgtttttt ctaaggcttt cgtatcggct
    18601 ttccttttat ctttgagttc aaaacgcagt ctggtggcac aatgggttaa gctgaccaca
    18661 ttttcttttc ccccaacctg ttgcaataca tttcttccta cttctctgta atccattatt
    18721 ctctctcctt aaagcagtaa aaaagaccaa gtcaaaccac gccccaatca acattgaagt
    18781 gttagtttgt cttggtcttg cctgcataat cagtaacatg ccaataacgt tattttgctg
    18841 taatccaata atagcacatt atgatagcgc aatcaacagg gaatttaatt ttttatcttt
    18901 tcgtttgacc gtatagggta atataacagt tagatcacat caactagggt atatctgcgt
    18961 atacttactg ctaaaataag tatatctaat attaactata ccagtaaaac cattcctaca
    19021 taaccagcgg gaaattccca aaatcgtgaa agcatcaaaa atgcatataa cggatcacac
    19081 aacttcaaca attctgctct tgggaaaaat tgatgaattt ggtgagtaaa ggatggaata
    19141 aacagcattc caacaatata ggcaacaata gtgatacttg tttttttggt aaaaatcagt
    19201 aagcaatagc caatttcaat cattccgaga tataaaggta tcaatagaat gatttgggct
    19261 agtattctta aaaattaact agcaaatgtt taaactgatt tatttctaat ctacatgtag
    19321 taagtatttc aattccacca attatcaata aaaataccaa acatatccat aataattttt
    19381 tctttttcat ttcttttata ttgttataac agtgaataac ataaaaattc ccgacaatca
    19441 ttgtaatatt tttttgtcac ctgtattagt atttaattta taggaaacta taaaaactcc
    19501 ctagtgaatt tacacctttg ggagttcctg tttggacggt atgcataata catttaaatc
    19561 aatttaacat taacacctca gcatatggta tctccacata tatacggtct ttctttggat
    19621 agtactcaat atttatgttg atagcacttc cattgtctaa tatagcttta gcccggtatg
    19681 tgttatctcc attttcatga gtaatcgtca catcatcaca tgatcgattt aagttgtact
    19741 gttcttgtaa aatctgtgtt accaatggtt ttgatgactt ctctaatcca gatgtacctg
    19801 tcatttttga aaaaataata cctataatag ccaagacaac aatacctata atgacaaact
    19861 tcttgttcat atttacttta ttcaactttt tatctcctat ctccaaaata atttgttctt
    19921 atccctctta tcagtacatt gacatcccta aaagtacacc aaagattgct ccagctaccc
    19981 ctgctatcat caaaatcgta cccattttct catctttttc tcgatataag tagcccataa
    20041 taacagcaac agcaccaaat acaacaggaa taaatgctaa cgatactacc atagaaatcc
    20101 aaccaataat caaatacagc ttccctgtct cttgtccatg tacgatttgt tccttcattt
    20161 cattaattgt ctgctcagta cttttcttta ctgtgtctgt tccggttgtc tcatctgttt
    20221 cttgtagtgc tccacaaaac tggcaatact tactttcatc tgttatttct ttgccacatt
    20281 ttctacaata catgtcttct cctcctaaaa tcattcaatt gcttattagt taactgttta
    20341 ctttattaaa tacatcagaa aacagctcat tcattggtac acttaatatc tcagaaagca
    20401 tcttcatttg atagggttta ggaatcgttc tccctgtttg ccacgaactt acacattgct
    20461 gtgtaacatt catttttttt gctaactccg tttgagaaag tcctttttct tttctgtatc
    20521 ggctgagatt acttactttt ttcatgattc tcccctcctg ttttattata tacaacaata
    20581 tgatgtattt caactcataa aatataaaaa acttcaaata ccattacaaa tacaacaact
    20641 tgttgtacta tactgagatg ggggtgtatt aatgaaacag aaacaatatg caaaggtcat
    20701 tagagaaaag cgaaaagctc tcgggtggac acaaaaagaa ttagctgaaa aaatcttctc
    20761 tacacaacag gcagtagcaa gatgggaaaa ttctgtaaca gaacctaacc tcgatagctt
    20821 aacagctctt tctagagccc taggaacccc tgtaagccat tttctagaca atgttgacat
    20881 agattatgag gaagagtttt tggctctcta tcgctctctt agtactgaag atgcaattcg
    20941 aacaattgac tatatgaagc tattaaaaag gcaagaaaat gaacgcaatc agctattgaa
    21001 agattgaaaa ataaataacc caccgaactt taacggtggg tgtattgaga tgaaataaga
    21061 ttaagagcta tggataacct gtctagttta atataattgg gtctcttcta ttccttactc
    21121 tccgtccatc atctcttgtt acacttgtaa attgataaat aggaactaat tcaactcctg
    21181 ataatatcaa ttcttttatt acttcatcta tccctgcccc tttcgctaga tatactaaaa
    21241 aactactgct tgctttcaca ttgtatagtt tcttaatatc cttagaagct cgttcaagag
    21301 tgaatccttg tttaacaaag tagcctctgt tttctattaa agcatttttc acgctgttat
    21361 ccctgacatt tcgtaggcgt tcacaaacag tgttaataag ttctttttct gtcattagat
    21421 atgtcccttg ccacgataag ccctgtaaaa cttcatgtgc ttcatctaaa atcaattgtt
    21481 tttccttgct aagttctgat cgggaatttt gaggaaatac tcttttagcc tctttttcgt
    21541 taaaacatat cttaacccat tcataagaaa aatattgata tgtaaaatga aatttttcca
    21601 gaagtggaag gatatcttca ttcaattttt gaatcacaga agttgcatta aaaacgtcta
    21661 atcgatagac attgcttctc ttatgtttaa agtttcctaa cgctttctct tcctttaacc
    21721 tatttaggta ttcgagcatc ttcaaaggaa catcttcatc atctaatttt tttatcaatc
    21781 ctataaaagt cataagtgat agtaacttat tacatttatc tatgtcataa gttttaccat
    21841 agtaagaact cattttttta gctaattgtt cccccgacaa aatacaagtc acactttcct
    21901 tactatcatc aaaaaaatta acatcagaaa gcaagacatc atagacacgt cgcatttcct
    21961 ctagataatt ccttattgtc ctgaaaagtt tgtctttcat tgattcaaga gtgtcaaaaa
    22021 gtacttttga ttcctctttc aattttcgaa aatgtgtagg atacaagtct aggttctcta
    22081 atagccaatt aaatgtttct acccgactgt ctttctttaa aattagcatc acaactccaa
    22141 taaaattttc actgatatca caattactgg aatgacaata aaaaatggaa tgcccagttt
    22201 tactggtaaa aatactagca gagggattat gatcgttgtg aaacagacat cttaagtttt
    22261 tacttgactt aagtaattta ttcatatcaa tggttaacaa tctttcataa atctcattaa
    22321 aagacatgtc ggaggaatca aacaggcagt ctttaaacca ctgtttcatt tcttctttgt
    22381 cgtcattttt tatcaactta acaaaatcgg tttcattgac tagttcatac tctctccctg
    22441 aatcactttg acaattttta gaacatgtca tggtaaaatc aatcggaagc tcagtaatat
    22501 ctaacatgtt gtcttcatta attattatca ctgcatctga cttatatcct ccaaaaaaca
    22561 ttctattcga agcatttaca ttgtcatcac acccggggat gttctttttc aagtattcgt
    22621 aaacagcaac cattttacca tagtcagtaa ttgcttcttt taatagaaag acaatcttgt
    22681 atttttctag attttcagag taccgaatac ttttttgtat cgcaaacgca gaattatgaa
    22741 ctaaattgat agataaaacc ttttctatgc ttaagtatct actatcatct ttactcaaat
    22801 ctactacctt ccccttctca tctttaacag tgttatcgac atccagaata attactctgg
    22861 tttccacaaa actctccttg tcattgcctt tattctttgt cttgcttgga ataaatgatt
    22921 gtgatgaatt tagtatctct acaaactcat ctcttgtaac ttctctatag ttatctaaac
    22981 acctatctct tacatcagtt ttttcttttt ttgtaactgg cttctctaat aatttttctt
    23041 tatctacata aattggaata ttaatcattg atgtttccat ttgtgtttcc tccttataaa
    23101 attctgttaa agactaaaac atacagataa taaaaacaaa ctctttatta ctatatgttt
    23161 tagtcttatt tgtttactca acctttatac cgttccatga aactatcaat atcagatttt
    23221 ttaaatctaa tgaccgtatc aatttttata acaggtaacc cgtattgaga aacatatttt
    23281 ctaatcgttc ctactgatac acctagatat tcacttgcct gctgatagtc catatactct
    23341 agagtgactc cagaaataat gtcttctagc ataggcttta gcactgtaat tatctctttc
    23401 acttgttgtt caacttgtat agaaacaatg ctatcaatga tttgtagaga catatttccc
    23461 cctcctttcg tgattgcttg aacaaatttt ctaagaatta tcttcgtatt ttttgctcaa
    23521 aataaaagta ttttgttatt aaccgattaa tcaaacaaaa aatgattacc agttcatcga
    23581 catacacata atacctcgat aaaaaagggg tgtcaacagc tttgtaaaat ttatttacaa
    23641 cacccctttt ttgaggtata ctaattttag gaggtgtggt caatatgata aaacgtccac
    23701 caataaatta tttagaacga aaaaagatac tcggaacaaa aataaaagct ataagaaaga
    23761 gtaaaaaatt aacacaacca gcatttggtc ttatgattaa caacggtcaa ttaattgaca
    23821 aaaaaactat ctacgaatgg gagaagggaa cctatttacc aataccagag cgattatccc
    23881 gaattgctga tttgggaaat atgagtatag aggagttagt ttgtggcaat gtagaagaat
    23941 atattttggg aattattctt tatagagata gcattgtact agatggtata acatttccag
    24001 ataaaaacct gttccagcat cttcgtcaac agttcccacc agtacactct aatttagata
    24061 catggctgga tagatattcc aaattagaac cagagatgca agaatttatt gctaacaaga
    24121 cttgtaataa agttaaaaat gaaaaaatta gtcttttcaa tatcttaaaa atagaagaat
    24181 tattcatcaa tgctattgta gaagaatttg acaataacat tcttttcctc actagcagta
    24241 tagaagaact actagaaaga atggtagatg aatggctacc tattcaattg aaagacatgt
    24301 cttatcccga agaagctgtg agagaaatta ctgataatat aaacaagcta gaacaaacga
    24361 tttcatcaat tgggaaaaaa tacactaaga aaaaaatgaa gggaggtgat accatctagt
    24421 ggtaagtact ccttgtaaac taagaggaga tgatttaatg gcaaaaatat caaagtacaa
    24481 aaagaaaaac ggtcaagtgg cgtggatgta taaaggacac attgccaccg atccacgaac
    24541 tggtgaaaaa ataaatacta cgagaagagg ttttagtagc aaacaagaag ctcaaaaaga
    24601 ttatgacgag tacagacatc aaatattata cggtgttaaa aagaaagcac ctgatatgac
    24661 atttgaagac ctgtataatg aatggataac tcatcagcgt accagtgtta aagcttcaac
    24721 aatcgcaatc tcagtaagat acgcaaataa tcaaatactt ccagcatttg gtaagcttaa
    24781 actatcgaat atctctgtcc catattgtca aaaagtggta gatgaatggc acagtaaata
    24841 tgaaagttac gattatatga gaaaacagac tgcacaaatt ttacgctacg gggttgcaat
    24901 gcaatatatt gacaataatc ctatggaaaa aacacttctt ccacgaaaaa aagagtatga
    24961 gaaaaatcgt aaattttact caaaagagga gcttaataat ttactggatg cttttaaaga
    25021 ttttggtaac atgaaacaat acgccttctt cagattattg gcatacacag gtatgagaaa
    25081 gagtgaagta ctcgctttac agtggaaaga catagacacg tttaataaag aactacatgt
    25141 caataaaact cttgctgtag atgaatttgg taaagttata attcaatctc cgaaaacaag
    25201 agcttctaga cgagttatct ctttagatac tgagactctt tctatcctca acaactggaa
    25261 attacagcaa aaagaagagt atttaaaact tggatacaat actagctcta aagagcaaca
    25321 tgtttttaca acagttaaaa acactctata cattccaaac actgttaatg attggttaag
    25381 atatattttg aaaaaatata acttgcctcg tattacacct catgggttta gacatacaca
    25441 tgctagttta ttattagaag caggagaatc tgtcaaggta gttcaacaac gattagggca
    25501 tgaaaatagt aaagtgacat tagacatcta tgcacatatt acaaataatg cacctaagaa
    25561 aacaggtcaa gactttgctg atatgatggc tcatcagtaa aagagctggt caaaatctgg
    25621 tcatttctag tatacaggca aaacaaaaaa gctagaaccc ttatatatca agggttctag
    25681 ctcctataac aggtttaccc aacactgcct tccatctcat aactgatcaa tcgattgagt
    25741 tcgactgcat attccattgg aagttctttg gtgaatggtt caacaaagcc catgacgatc
    25801 atttcagttg cttcagattc ggtcaggcct cggctcatca gatagtagag ttgttcttct
    25861 gagatttttg agactttggc ttcgtgttct aaagagactt ggctgttgtg gatctcattg
    25921 aatgggatcg tatcggattt cgaccattcg tccatgatga tcgtgtcaca ttcgatatga
    25981 gagatcgaac ccgcactgtt tttggcaaag gttacttgtc ctcgatagtt tacttctccg
    26041 ccgtcttttg aaattgattt agagacgata gagctagatg tatttggtgc attgtggatc
    26101 atttttgcac ccgtatcttg gatttggttt gctcctgcaa aggcgatgga gagcatggtg
    26161 cctcgtgcac cttttccatc taagtaaaca cttgggtatt tcatcgttgt ttttgcacct
    26221 aggtttccgt cgatccattc aacggttgcg ccttcgtatg cttttgctcg tttggttact
    26281 aagttgtaca cattgtctga ccagttttgg atcgttgtgt aacggcaata cgcatctttt
    26341 cttgtaaaga tttctacgat ggcggcatgc aggctgttac ttgaataagt aggtgccgta
    26401 caaccttcga catagtggat gcttgctcct tcgtcaacaa tgatcagcgt acgttcgaat
    26461 tgccccgtgt tttccgcatt gatccggaag tacgtttgta atggaacatc gactttgacg
    26521 ccttttggca cgtaaataaa ggtcccacct gaccatacgg ctgaattcag cgctgctaat
    26581 ttgttgtcgg ttggcggtac aagttttgaa aagtactctt tgaataattc agggtattct
    26641 ttcaaagcag agtcagtgtc agtaaagacg attcctaact tttcgaattc atctttcata
    26701 ttatggtaca cgacttctga ttcatattgt gcggaagcac cggctagata agctcgttca
    26761 gcttctggta taccaatctt ttcaaatgta tctttgattt tttctggtac gtcttcccag
    26821 tcacgagcgg gtttgtcact tggtttttgg taatatttga tggcatcgaa atcgatgtct
    26881 gaaaggtcgg ggccccatgt ttgcattggc attttgttaa atgtttctag agatttcaga
    26941 cggaattcaa gcatccattc tggttcgcct ttgattcttg acatctcacg gatcacttct
    27001 tctgtcaagc cttctcctgt actgaaaacc ggttcgacat catcgtgaaa cccgaattta
    27061 tattcttcta attctggtac gcccatgttc tcactcctct tccttgtggt aatgatggaa
    27121 agctgcggta ccatcgtttt ccattgcttt tcctagtgct ttccaggcaa gtgtcgcaca
    27181 tttgatccga gcgggaaatt tagctacacc accaagcata gcagcatcgc ctaattggtc
    27241 gggatctttg acttcttttc cttggacaag ctgtagaaag tctacagtta aatgttctgc
    27301 ttcttctatt gattttccga tgacggcgtc tgtcatcatc gaggcacttg ccgtactgat
    27361 ggagcagcca ctgccgtcaa aggcgatatt tttgatgatt ccgtctttta catcgacttg
    27421 caattcgatc acatctccac atgtggggtt gttcaattcg atcttgcttg tcgagtcagt
    27481 caagcttcca cgatgatgcg gatgggaaga atgatccaag atcacttgac ggtataaatt
    27541 gtctaattta gatagtccca tgttggaaaa actcctttgt tgccatgatt gcttcaacta
    27601 aacggtcggc gtcttgcgtt gtgttgtaga aataaaagct cgcgcgagca gtcgctgata
    27661 cttcaaggta cttcaatagc ggttgcgcac aatgatgacc tgctctgacg gctacccctt
    27721 ccatgtctaa agctgtcgcg acatcatggg gatgtaaccc atccaaattg aatgcaacga
    27781 ctcctgtccg aaaattcggg tcttgtggac cataaacagt caatccatca attgcttgta
    27841 attttggcaa cacataagcg gctagttctt cttcatatcg atggatattg tccataccga
    27901 gtttttgcaa ataatcgatt gcatggccta atgcgatagc gccggcgata tttggtgtcc
    27961 ctgcttcgaa tttccaaggt agttctttcc atgtactgtc gtataattcg acaaaatcga
    28021 tcatttcgcc accaaattca acaggttcca tttcttctaa ccattttcgt ttaccatata
    28081 aaacaccaat acctgttggt ccgcacattt tgtgtccgct gaaagcaaag aaatcacagt
    28141 ctagtgcttg gacatcaatg gccatgtgcg gggcagattg agcaccatct actacgatga
    28201 ccccgccgtt ttcatggaca agttcagcta attcttgaac aggattgaag acacctaaga
    28261 cattggatac atgagcaatg gagacgattt tggttttgtc cgtaatcttt ttcgctgcat
    28321 cctccatatc caggaagcct tcttctgtta gttcgatata tttcaagaca gcacctgttc
    28381 gttttgccaa ctgctgccac gggataacat tagaatgatg ttccatgtaa gagagcacta
    28441 tctcgtctcc tgctttgata tagcggtcgc cgaaggttct ggctacccaa ttcaagcttg
    28501 tcgtcgtacc acgagtaaac agcacttctg ctgtttcttt tgcatggatg aacgcccgta
    28561 ctttttctct tgcttgttca taatcatggg tcgcgcgttc tgctaacgta tggacaccgc
    28621 gatgaacatt ggcgttatcg tgttcgtaat agtacgctaa tgcctggagt acttgttttg
    28681 gcttttgggt cgtcgctgca ttatccagat aaactaaagg ttcatcattg acgtcttgaa
    28741 ataagatagg aaaatcattg cgtagctttt tagcatcgat catccattca acttcccttc
    28801 gattacttcg actaattctt tccgaacttg ttctacaggg attgctgtta agacagaacc
    28861 gaggaatcct cgaatcacta agcgctctgc ttcttcttta tgcaatcctc tactcatcag
    28921 atagtacatt tcttctggat caactcgtcc tacactggca gcatgtccag cagttacttc
    28981 gttttcatcg atcaaaagaa taggattcgc atcaccacgt gctttatcag aaagcatcaa
    29041 gacacgactt tcttgttggg catctgcacc ttttgcccct ttcaagatgt ggccgatacc
    29101 attaaaggtc aatgttcctt tttctcggat cactccgtgt tgcaaaatat gcccgatcgt
    29161 atgaggggct ttattggtta ctcgtgtatc gattccttgg atttgtctgc ccgagctgat
    29221 tgccacgatt ttgacttctg cgtgtgcacc ttctcctgct aaatctgagt caaagtcagc
    29281 gatcacatga ccgtcattca tgacaccaat tgcccagtcg acagaagcat ctcgtaaaat
    29341 gtgcccacgg cgattcatat aagaagtaat attttctcct aattgatcta cggctgaata
    29401 tttgattttt gaaccagctt tagcgatgac ttccacgatg atattacctg atgattttgc
    29461 tacttgttct ttggttgtct ggaaacgttc cagataagaa aattcactat gttcatctgc
    29521 cacgatcaac acatgcttga agaagtgctc atcggatgcg ccatcttgaa tgaataagga
    29581 ctcgagtggt tcatcgatca cgacattttt aggcacatac aggaaaatac cgctgttcat
    29641 aaaagctaca tggagtgcag tcaattggtc ttcgttcatg tttactgctt ttttcatata
    29701 atattcttgg actagttctg gatattcgat cattgcagtg aacaagtcgg taaaaatgac
    29761 tcctttttct gctaattcca caggcagttg ttcaaaaatc gtccatgaac cttgttggac
    29821 aagcacagga ttgtctttca ttgcatcaaa tgccggaatc gtgcctgttt cactgatggt
    29881 ttgtgtttct ttcacatcaa ataacgacca gcgatggaat ttcacccgat cgatatgcgg
    29941 caatggcagt tcatctgctt tcgctagtgc gtcttgacgg aaggttctca tccaatcagg
    30001 ttcgccttta cttaaagaaa aatcatttac cgcgtcaaga taatcgagcc agtttttttc
    30061 tttcattctt gctccccctt acgcttcttc ttctttatag tcgattccta attctttact
    30121 gataccagcg tagccttctg cttccagacg tttagctagg tcagcattcc ctgtcatgac
    30181 tactcggcct tccatcatga tgtgaacgac atcaggtgtg atatagttca ataatcgttg
    30241 atagtgggtg atgatcaatg caccgaaatt gtctccgcgc atttcattta ctcctttagc
    30301 aacgactttc aaagcatcga tatccagacc tgaatcgatt tcgtctaaaa ttgcaaatgt
    30361 aggttctaac atcaacaatt gcaggatctc attccgtttc ttttctccgc cagaaaaacc
    30421 ttcgttcaga taacgttcag ccatttcttc tggcatattt agtaaagcca ttttttcatc
    30481 taactttttc aaaaatgcca taacagaaat tttgtcgttt tcatcacgtt tcgcattgat
    30541 cgctgcacgc atgaattccg cattcgtgat acctggtatc tcacttggat attgcatagc
    30601 taaaaataag ccaagtcgcg cacgttcatc tacttccaag tctaaaacat ttttcccgtc
    30661 aaacaaaatt tcgccttctg ttacttcata gtttggattg cccatgatag cggcagataa
    30721 ggtagatttt cctgtgccgt taggtcccat gatggcatgg atttctccgg ttttcattgt
    30781 taggtttacg cctttaagga tttctttttc ctcgatcgac acatgtagat tttttatttc
    30841 taagacagac atgcattata cccccaaatt ctctttcgtt ctcactcatt ttagactaat
    30901 tgagaatgaa gcgcaatgac cttccttact aaaatcaaat atcttttatt tattattaaa
    30961 caattttgat ttctaaatta gaataggtat aaaaaagatg agaaataagc cattacagac
    31021 ttatttctca tcttttttgt tccatacctt attttgcatc agcctttact ggtttaacag
    31081 agccgcccca tttgtctgtg atccattctt ggacttcttt gcggtgaagc acttcaatca
    31141 gttttttgat tttgtcactg tcttcatcac ctttacgagt cgcaataata ttcacgtaag
    31201 gagagttgtc cttttcaagc aatacggcat cttctaatgg attcaaccct gcgccgtaag
    31261 cgaagtttgc gttgattgca actaggtcac cttcatcatt ttggtacgta gaagctaaaa
    31321 gtgatgggtc gatcgtatgg ttgaacttca agttcttagg gttttcttca atatcatcga
    31381 atgttgctgt ttccagatct actccatctt tgactttgac taagccagca tcttgtagaa
    31441 ttgtgatgat gcgtccccag tctgattcag aatttgaagt aatgattgtt gcaccatctt
    31501 ttaattcgga aacatctttg acttttttag agtacaagcc cattggttcg atgtggacag
    31561 caccggcatt gacaaagtca tatccttttt ctttgatttc tttgttcaaa taaggaatat
    31621 gctggaagta attggcatca atgtcgcctt cttccaaaga ttggttcggt aatacatagt
    31681 catcgaattt gacgatttca agatcgattc cttcatcttt caataatggt ttcacatgtt
    31741 ccaaaatctc tgcgtgcggt gtcggtgaag cgccgacttt caatgtcgtg tcgtctgctt
    31801 tatttgctga atctttgctg tcgctgttgt taccgcatgc tgctaatcct acagtcagca
    31861 aaagcgctgc tgcaaatcca aaaattttct ttttcatatc ctacatcccc ctatatccta
    31921 ttatcttttg tctgttttct tagttaagaa atcaccgatt ccttgaacga taaatacgat
    31981 tagtaaaata attaaagtcg caacgaatgt cactgttaga ttaccacgct ggtagccttc
    32041 ctgccaagca agacctccaa gtccacctgc gccgatcgcg ccggccatcg cagtaaatcc
    32101 gatcatcgag atcgttgtaa ctgttaatcc agaaatcaat gcgggaatac tttcaggtat
    32161 caagaccttc cagatgattt cccagtaact tgctcccatt gcatctgctg cttccagtac
    32221 accagggctg acttcacgaa aagcaatctc tactagacgg gcataaaatg gtgctgcgga
    32281 taaaacaaga gctggaatgg ctgcttgcgc acctagcata gtgcctacga ttgctttagt
    32341 aaatggaatg atcaaaacca tcaggatcat aaatggcgtt gaccggaaaa tattactgat
    32401 gatcgatacg attccataaa tgattcgagc atacggtttg tttttgcgtc caatagagta
    32461 aaggatcaat cccaaaacta atccgatgat cgtcactaag accattgaga ttaatgtcat
    32521 atatagtgta tcaaggatag cctgattcat cgtttccggg ttgatttgtt taaaatctag
    32581 atatgtttct gaaaagcttt tatccacgat ggatcacctc aattcctact ccttgttctt
    32641 tgatgaacac ttctgcttga tctaattctt tggtttcacc agtcatcaag acagtcaatg
    32701 aaccaaagga accttctttc acttgtttga tctttccata cacaacgttg acatcgatgt
    32761 tatagcgtcg aatcgcttgg gaaatgactg gctcgttggc atttctttcg ctaaaggtca
    32821 aagttgccaa acggccgctt ggattttctt tgatgagttc agctaatagt tctgtcgaat
    32881 cagcttctgg ttcgatgtct tgttgtacaa agcgtttggt tacttctttt ttcggttgtc
    32941 tgaagacagt caatacgtct ccttcttcta cgactttccc caactccatc acagcgactt
    33001 tgttgcagat tttgcggatc acattcattt catgtgtgat caagacgatg gtcaaattca
    33061 atttcttatt gatcgctaac aataagtcta atacttcttc tgttgtttgc gggtccaatg
    33121 cgctagtagc ctcatcacaa agcaggatct caggatcatt tgccaatgct cgtgcgatac
    33181 cgacgcgttg tttctgtccg ccggaaagtt gtgaaggata tgcttgtccg cgtccttcta
    33241 acccgactaa acgcaacagt tcttctgccc gttctttacg tttgctcttt ggtacgccag
    33301 ctaattccag cggcagctgg atattttcaa gtacagttct tgaccataac aggttgaaat
    33361 gctggaagat cataccgatt ttctttcgga atgtacgtaa ttctttgttt tttaattttg
    33421 taatatcttg atcgtttacg atcacttcgc cgctcgtggg aagctcaagt ccattcagga
    33481 gacggactaa tgtacttttt cctgcgccag aatagccgac gattccataa atatcccctt
    33541 tttcaacgtt tagtgaaacg tcattgacgg cttggatgtt tccgtttttc ccattgaatg
    33601 tctttttgac attcgttagt tggattaatg acatgccatt cacatctttc tcttatgatt
    33661 tttcaaacaa aaaagccttc gccctcttgc cttaagcaaa aggacgaaag cttgaacttc
    33721 cgtgttacca ccttgattta ccgttacttc gcaataacag cctcttccag tacttggact
    33781 tagcttcttt ccgtatactg tcgcgctgta acgggcactc ccgtagtaag tttgcttacg
    33841 ctcgcttact ctgctctaag accatcttcc atctcatttt cgttacccat tttcagcaaa
    33901 cagggctctc ttcaaacgtc cgtgacatgt actcttcttt tcaaagcttt ctcgtttcat
    33961 ataggattgc ttatgattat agcaactgga agaatcagtg tcaacttttt tcaagtgatt
    34021 ttctgatttt ataattcgtc aaattctttt ggatcaacat ctttgaaact taagatccac
    34081 gcattcattt catctgaatc attcaagata ttgatatctt caccgatttt atcattaaca
    34141 gaagaaactg tacccgttag cgggctggca aattcattga ccgctttttc cgcttcaact
    34201 tcgatcagag gttcatcttt ttttagcgat tgtcctgctt ttggcagatt ggcaaacgtg
    34261 atgtctccaa gttcttcttg cgcttcattt gtcaaaccaa cacagtattc ttttccatta
    34321 aataagatcc aaaggttgtc ctttttcttc aaatgcttgc ctgccatttt atatcagttc
    34381 tccttcataa attttttcat tgaatccaat tgcaacaagc tggtcctctc gtaagatcag
    34441 tggtcgcttg atcagcatac catctgtcga taagacttgg ctcgcttttg aagccgtaaa
    34501 ctcgcctact tgatctttca gtccaagttc ccgatatttc atcccgcttg tgttgaacag
    34561 gcgacggatt ggtacttgac tcgtttctag ccaagtctca atcgttgagg ccttcggtac
    34621 ttctttgatc atatcaacgg tttgataact aacgccatgc tggtctaacc acgctttggc
    34681 ttttttgcat gttgaacatt tcgggtacca gtaaaaggtg tacaattttc ttacctcctt
    34741 gaagatcttg caggtgcttc attttggtat ctcatcgata atatcaaccc gataccgatc
    34801 atgttcccta aaatcgagga tcctccctga ctaatgaaag gcaatgggat ccctgttaaa
    34861 ggaagtaatc cgatatttgc tccaatattc tcaaagacat ggaacagcag catcatgatc
    34921 aaaccgcttg cgatataggc gtaaaattca ttattcgtat cgaaacacac ccgaatcata
    34981 cgatagatta aaataaaata caagaaaatg acgaatgcac caccgataaa gccgaagttt
    35041 tcaccgataa cggtaaaaat catatcggac tcacgaacag gcacatagat attgcttacg
    35101 ttgaatcctt taccaaacaa gccaccggta ccaattgcca aaagtccttg ggccggctga
    35161 taactcattc ctgacgtatc atggaaaggg tccagccatg aatcaatacg tgcaaactga
    35221 taagatttga atccaacttt ataaagcaag tctcttccgc cttcagtcgt aaccaagaag
    35281 atcgttcctg ctccaacgac tactgccaag attactacag gtgcgatgat gcgccaagag
    35341 attccagaca tcaggaaaat cccgccaaag atcgcaagaa agacaagcat cgttccaaag
    35401 tcatcttggg ccatgatcaa tgctaaaacc ggtattgtca cgatcaagag cttcccaatc
    35461 agccaaaagt ccgatttcat cgtccgttct tttacttgcg tattatgttt agttacgatc
    35521 agtgccatca tcaagatgta cgcaatcttc atcaactctg ctggctggaa agtaaacgca
    35581 ccgaatcgaa accagttttt agaccctgtt gaagcagcta aattctgatc atagaatttg
    35641 agcaagagtc ccattaccac taaccccaaa ccataaaggt aaggagtcag tttccagatc
    35701 catttggaat tcaaatgcat gatgacaacg attgctgccg ctccaactaa ataccagaca
    35761 acttgcatcc ctacgccttt caagactgct ggattgttcg gatcatggct aagtgctaca
    35821 tataatgaga gcagaccaat caaacataac agaaagactg gtaaaatcac gccataatca
    35881 atacgattat cgttcgaagc ttttaatttt ttttccatgt ttcatccctt ttcttaaaaa
    35941 ccatactgct gattattata gcggtccagt tagataatgg aaagtctttc catagacatt
    36001 tgggaaaaat taactttccg tttgtgcagt tgcttgagca aatgcagtac aaaagcctac
    36061 tgcttatctt tagtgtatca tagaactttt tttgatacat gcaattatct accagctgag
    36121 attctcctcg ctgtgagaag tttctgtgct ttcattgtat cgaaggatct cttcggcaat
    36181 ctctagcagc tgatcagaaa ccagattggt tcgttggatg aaataaaaat atcgatggaa
    36241 attcaacgga gtcattggca cattttctgg aacagagcgc ttagacaaaa tcgagcgacc
    36301 gattccttcc ttcagcaacg caacaattac atcgttactt ttcacgatca tcttcttatc
    36361 ttggatattc atctcttcaa aataacgctg cgtgtaatga aaaacaccag agtttgcttc
    36421 tcttacaagc cacaatttgc tttttgggtc cccagcgatg actagctggt cttccatcaa
    36481 agagtatctt tgtacgccgg aagtctctaa tggcttttcg ataaaaccaa aatcggattc
    36541 atgtttcgct acttgttcta atgattcatg agaattcgtg atttcgatct caaaatgcac
    36601 tttaggaaac cttttgatca gatgtttcat caactgaggc aaaagataga cagcaaaggt
    36661 gtgggaagca acgatccgac atacttcttc ttcattttgt tcattgagca atttttgctg
    36721 agtttcttcc cattcttcta acaaaaccag tgtccgttca tataataagt cggcttgctt
    36781 agttggaagc atctcttttc ttccgttacg ttgaaaaaga tcgatattca attcccgctc
    36841 caactgtttg atttgattcg agactgctgg ttgagataaa aacagtactt ctgctgctct
    36901 ggaaaaattt cttgtttcat atactgctcg aaaagtcgtt aataacttaa acatggcacc
    36961 acttccataa ctttttatga taattactat aaaaactatt tgatttaaat atacaaaaaa
    37021 ccatctataa tagaaatgta caacaaaaca gttgctgatc caactagtaa cttagatgaa
    37081 gacaatcagc tgtctgcttg ctcgtaatca tacattccta ttcattttac aatgtattcg
    37141 ttcatcttgg tactaaaatc tattaaataa aaaggagaat attatatatg aagaactttt
    37201 tgaaaattat tccgatcccg atctgcgggt tgatccttgg gttagcttcc cttggaaatc
    37261 tgtttaaaga ctatcaccat gttgctttag gcaatctaac tggcggtatc tctatgattc
    37321 ttatgatatt catttttgtt aaattgttta tcttattcga acacacgaaa caaacgttgc
    37381 atgatccaat catcgcctcc gtttcaccaa cttttacgat gtcactcatg gttatttgta
    37441 cttattttgt ttcatatgaa agtattgcac caattgtcaa attcatttgg ctgtgcgctg
    37501 ttatcttcca agtgatcctt gtcttttatt tcaattatca ccatgttatc aaagctgatc
    37561 tttcaattga agcaatttat ccaagctggt ttatcattta cgttggattt ggggtcatca
    37621 ctgtaactgc cgggaacttc ttcccactta ttggaaaaat tttcttttgg atttcattga
    37681 tctgctatgc gttcttattg ccgatcatca tcaaacgtgt ctttttcgta agaaatatgg
    37741 ctcatccgac acgtccgctg attacgatcg ttgcagcacc tggttctctt tgcctaacag
    37801 gttatcttaa aaactttgct gaacctaatt tttatcttgt cgtagcattg ttcgctttat
    37861 ctcaagtatt gtatttcgtt gtgctgtcta tgcttccacg tttgttcaag cttcgttttt
    37921 atccaagcta tgcagccttt acattcccgc ttgttatctc tgcaacagct atatttacta
    37981 ctgttcatta cttcaacagt ttaggtatct atagtagcgt attggatttc ttaaaagttg
    38041 cggaatgtct gtttgcaagt gtagttgtct tctacgttct tgctcattac gtacgtttct
    38101 taattaaaga acatagcaaa catggaaaat ttgtttctaa aaaaatcgct taataatcga
    38161 ttcaataggt tgtgaaaaag ctgttctgca tcaagtaata agaacggcca aacaaaaaca
    38221 gctgttcatg tttttgtact ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    38281 tttaccaaga ggtcgtaaaa gaagtggtca gcttcaagaa ataagaagga atttacgaaa
    38341 attgcttctc aaatttttgt gaaatttcgg cttatttccg tagaagctac ttctggaaca
    38401 ccgtttattc gaaacaagag gtcgtacaag acttttgtca cgacctcttg ttcttttgtc
    38461 aaaatactgg tttagtgcta ctatgttcga gtatgctttt agtatttatt gacgaaagga
    38521 accgatcaaa tgaaatcatt gtcacatcca ataaaatctg taaaggattc agcggtccgc
    38581 gaaatcttac ccggtttact gctttctttt gccatcgctg ccttcagcag gcttcttgct
    38641 atttatctgc cacagttagg tggagcgact attgctattt tattgggtat tttattagga
    38701 aatacttttt ttaaacagcc tgttctagca atcggcacaa aatttgctga aagcaaacta
    38761 ttagaattct ctgttttttt actaggtgca actgtcacag ctcaaacgat tgctcaaatt
    38821 ggtgtaaaag gaattgtttt tgtgttactg caaatgtcag tcactatcat cggtgcttac
    38881 ttgattggta aaaaactgca attcagcgat acttcttctt tgatgatggc tggaggaaac
    38941 gctgtttgtg gttcttctgc tattgcttca atcgcccctt ccatccatgc gaaagaggaa
    39001 gaaaaaggac agatcatcac actagtcaat ctactgggta ctgtgatgat gttgactctc
    39061 ccttttcttg gattacagtt gtttggtgga aatctcattg aaaaaagtgc acttttgggt
    39121 ggtacactcc aatctgttgg tcaagtagtc gctggggcaa gcttacttga caattctgtt
    39181 gtcgaattcg ccatgctatt taagattcta cgtattatga tgctcgttgt tgttgtctat
    39241 ctgtttgaaa aattcatgca caagaaacaa ccagctctgt ttgaaacgcc aactgatacg
    39301 cagaaaaaac gaaaattacc gcttccttgg tatgtattag ggtttgtgat cttttgtttg
    39361 gtcaacagct tgttttcttt acctgtagtt attgaccaat cggcacattt tatcagcagc
    39421 tggtttgaga tcactgcttt agcggcaatt ggtctgcgtt tagactttgc aaaattcatg
    39481 aaagaaggta cacgcttctt atcttatgga ttagctgtcg ggcttctcca aacagtgatg
    39541 gctttactat ttatttggct gatcaaagtt tagaatgaac atgaaaaaac agttgtgatt
    39601 ctcgcatgga acaatgtccg aatcacaact gtttttaatt ttgcagatat ccttctaaca
    39661 acaacaattg gcgtttgatt tcaaccccat ctattgcgtt tcccatatat cctgtcagtt
    39721 ttccattttg cccgatcact cgatgacaag gaataacgat tggcaatgga ttggttcgat
    39781 ttgcctgccc aatcgctcgc acagctttcg gactatcaac agcaacggct acttctttat
    39841 acgttcggat ttctccataa ggaatggtcg acaaagcttt ccagacattt tgctgaaaag
    39901 gcgtacctct ctttatagat aaaggaacat caaattcttt tctttttccc gcaaaatatt
    39961 cttcaaactg ttcttgtgtg cgcgttaaaa ttcgctgatt cgctgattcg ctttctgaaa
    40021 ttggctgaaa agatacagca gtcaatccgg attcatctgc atctaaccat aaaaagccga
    40081 taggtgtgtg tattttcatt ttgtttgctc ctagtcaata gactggaact tcctcctttt
    40141 tctctatcca aaactacttg ccaactaata aaaattgttc ttaaaagttt gataaaccga
    40201 ggaaacatgg taaactaaat acgttagttt acaaaaagaa tacctgttct tttacgtaaa
    40261 atcaaatttt aaaggtgtta tttaaggttt attaaatttt gaatgtaaac catatataaa
    40321 catacgattg aaaggataga aacgatgaaa gtatcaattg ttgtcccatg ttttaatgaa
    40381 gaaaaaactg ttcctctttt ttttgatgag gttgaaaaat tcaaaggtgc ccatgagttc
    40441 gaatatttgt ttattgacga cggatcaaaa gacggtacgt tagcagcaat caagaagcta
    40501 gcctcagctt atccttatgt ccgctttgtc tcattttcca gaaattttgg gaaagaagcg
    40561 gctctgtacg ctggattgca agcttctaca ggagatttag tgaccgtgat ggatgttgat
    40621 ctgcaagatc cgcccgaact gcttcctgag atgatcaacc ggatcactac ttctgatatc
    40681 gattgtgtcg gcacccggcg ttcgactcgt gaaggcgagc ctgcgatacg cagtttcttt
    40741 gcgaaaaaat tctaccagtt gatcaataaa atcagcgata cagaaatggt cgatggcgct
    40801 cgtgatttcc gcttgatgac tcgtcaaatg gtggatgccg tcctagaatt aacagaatac
    40861 aaccgatttt caaaaggatt atttagctgg gtcggattta aaacagaata tatttcttat
    40921 gaaaaccgcg aacgtgtggc tggcgaaaca tcttggtcct tctggaaatt attcaactac
    40981 tcgattgatg gtatcgtgaa cttctcggat gcaccgttaa cgattgcttc atttatcggc
    41041 gctttgtctt gtgtcggttc tggtattgca attttgttca ttattttgcg ggcattgttg
    41101 tttggtgatc ctacatcagg atggccttcc atggtctcga tcttcctgtt cattggtgga
    41161 atccaattgt tatgtcttgg aatcatcggg aaatatattg ggaaaatctt cttagaaacg
    41221 aaacagcgtc ctgtttatat cataagagaa acagaaaaag tgaagccaaa tgaattgaag
    41281 cctcaccaat aatatacgtc aaatgaagta aacatttttt taaatgaaaa aggactgtga
    41341 caaaagtctt gtcacagtcc ttttttccga ataaatggtg ttccagaagt agcttcttcg
    41401 gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    41461 cttgaagctg accacttcta tcacaacctc tttcatatct tattacttct tcattgctgc
    41521 ttttagtgct gctgctaatg ggctttcttc tggttcttct gcttgtgcat attttttcag
    41581 caagcgtcgt tcttcatgtt tggtcatttt cttcttctgc tcttttttat caagcaattt
    41641 ttctgtcgtt ccatcgtatt tacaacggaa ataagcaccg tttttacctt cgatgatttc
    41701 cattttccgg tgacattgcg ggcatcgatg attagagact tttggatctt ttcttctgcg
    41761 gtagttgcaa tctgaattgg tgcagacata gattttgccg tcacgtgtgt ttttctcgcg
    41821 cagattggcc ccacattctg ggcatttctt ttgggtaatc gagaaatctt gatattcttg
    41881 tttgcttgat ttgatctcat tcaccaagcg ttttgtttct ttttcgattc cattcaagaa
    41941 ggttcctgct tgtttctttc ctgcagcaat agcttcaagg tctttttccc agctttctgt
    42001 caaatctggt gttactaaag agggatttac caatactaat aattgcttac ctttaggaga
    42061 tacttgcagt ccttgagggg tacgttccat cagttcagat ttgattagtt tctcgataat
    42121 ctctgcacgc gtagctgggg ttcccagact gtatttttcc atttgaccaa gcaagcttgc
    42181 ttctgacaat ggtttaggcg gtgcagatag ttcttttttg atggcgaaat cgggattgat
    42241 tctcattcct ttttcccatt tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc
    42301 tgcttggacg acacgatttt gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac
    42361 ttttgtttgg ctgactttat ggggttgagc aaacagtcct aaaaaacgag agacaatcat
    42421 ttgatagatt tttcgctctt cattacttaa tttttcataa cgtgctctgt tttcagttgg
    42481 caaaagtgca tggtgatcgg tcactttact gtcttggaat acttttgtct gttggacgat
    42541 ggctccgttt ttcaaatact gcttgacctc aggagcaaag tcgctgactg cttgcaatcg
    42601 ttctttcatc gtacttttca tgtcatttgt cagatatttg ctgtctgtac gcggataagt
    42661 aacgatctta tgggtctcat acaaactttg tacaagactt aatgttttct ttgcagaata
    42721 gccatagcgt tgattggctt ctctttggat ctctgttaaa tcataaggca atggcgcaga
    42781 ttccgttttt aatttttctt ggacttctgt gacagttcct ttgcttgtac tgatcaattg
    42841 aacaagtttt tgtgcttctt cttgtgtttt taaagcaaat tgattttttt gaagcatttt
    42901 ggcttgttct tttccagctt gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc
    42961 gattttcttt tcttggtctc tcactaatgc tagtgtcggt gtctgcacac ggcctgctga
    43021 taaattatcc gcgtacttca cagttaaggc tctggtcaca ttcaatccta ccagccagtc
    43081 cgcttttgcc cgggcaagag cagattcata caatgcatca tattctttcg caggtcttag
    43141 ttgcttgaat ccagttttga ttgctttatc tgtttgagaa gagatccata accgcttcac
    43201 tggtttattg aaatgcacat actctaagat ccagcgagca acaagttccc cttctcttcc
    43261 ggcatctgtc gcaatgactg cttctgagac atcttttcgc tgagccagct gcttgattgc
    43321 ttttaattga tgtcctgttt taggaagtgg tttgattcct agttttttag ggatcattgg
    43381 caacgtgtcc atttgccagc tctgccattc cttattcaga tcttccggca ttttcaatcc
    43441 taataaatga ccaagggccc aagtaacgat caccttgggt ccttcataat aatttttgtg
    43501 cttttgatta gcaccaagca ccttgcttag gtctttcgca acgcttggtt tttccgctag
    43561 aattagttgt ttcataaaat tcctttctgc tttctactgt gatgggctga tgatttttca
    43621 acaacgcaag tcccttgtat tgctgtaaat catcgtcaca ctcttcacac cagcgctcat
    43681 ctccgtcatt ccaaaaggcg ctgagaaagt tactttttgc attgatatat tcataatttt
    43741 gatggatttc gttccattgt ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg
    43801 tttcttcttc ttcaatatct tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc
    43861 cgaacatggt aattacttga tacatactcg tgctccttct ttcagctttt ctagactcat
    43921 cttagtcact tttcaagaaa gttgcaagaa ttttctctta taaaagggtc atttaatgaa
    43981 catcttaaaa ttgttacaaa tgaatctgat tgtttaaggc aaagtagcga taaagttatt
    44041 gatcatccaa aaatagctgt cttctggatc gattgttggt tcttttgtaa agtatcctaa
    44101 aaaacgtaag gaaagataac cgtggatcat actacgaagt tccctgcttc gatgaacttt
    44161 ttcttctttc gttaattgaa aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg
    44221 gattccatct aataaaacag cttcattggt atgcggtaca ctgatcagta actcatacac
    44281 tgcttgattt gaaaaagcaa aggatcgata agtttctgca tagatctgca gtgcttcctg
    44341 accacttttt cccacaagag cccgtcgcag ctggtcatta agttcctgca gaagtttcgc
    44401 tgttagcgct gttttgacat cacgaatatt tttgaaatgg ttatacagcg agggagcctt
    44461 gatatccaag cgtttggcta aatgctgaaa ggttatttgc tgaacatcca tctcttctgc
    44521 tagttctcga aagcagtcga tgattttctc ttgagacaga tttctttttt tcatttccat
    44581 gtttcctctc ataaaataaa tccttcactc tttcagttta ccacgaaaaa gaaagagaaa
    44641 aaagagactt tcattgataa actacatatt gtagtttata ataaagagct gtagatgaaa
    44701 attttcagaa agaaggagtc taattggctg caagaataaa ggaaaaatta tttaggaaaa
    44761 gagaaacttg ggaacaaaac ttgtttgtct tatggtttgg aacatttatg gctggtatcg
    44821 gatttagttt agtcatgcct tttatgtcac tttacataga tacgttaggt aattatagta
    44881 cgtcacaatt gaacttttgg agcggattga ctttttcatc tactttcttg gtgactacgc
    44941 ttatctctcc ttggtgggga aaattagccg accaaaaagg aagaaaattg atgctgttgc
    45001 gtgcttcatt aggaatggca atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc
    45061 aattagtcgc attgcgtctg cttcaaggga ttttttctgg ttatatcagt aatgccactg
    45121 cccttgtcgc aactgggacg ccaaaagaaa aaagtggtca agttcttgga accttagcaa
    45181 ctggttctgt tacaggaaca ttacttggac cgcttcttgg cgggatctct gcttcagcgt
    45241 ttggctatcg ccctaccttt ttcattacag ggatcatctt atttttcgtc ttcttgttga
    45301 gtctgttttt cgtccatgaa aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca
    45361 aacagatttt caaagacttg aaatatccac acgtcgtcat cggtatgttc gtcacaacga
    45421 tgatcatcca agcatccaac aattccatta gtccaatcat cagtctgtac attcgccaat
    45481 tgctgcatgg acatgggaat gttacgttga tcagcggaat cattgcttcg atacctggga
    45541 ttgccacttt gatagccgct ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa
    45601 gaatacttgc gattggtttg ggttttgcga ttctggttta tatcccaatg gcattcgtca
    45661 cgaatgtttg gcaattagct gccttacgtt ttttgattgg tatctctgat gcttgtctgc
    45721 tccctgctgt ccaagcattg atcacaaaat attcgcctca tcaagctgct ggacggatct
    45781 tcagttataa ccaatctttc caagcaactg gtaacgtagt agggcctatg atcggctcca
    45841 gtgtttccag tgcatttggt tatcgtggcg tctttctttc gacttctctt cttgttttgg
    45901 tcaacttctt gcttgtacga cacaatacac aagagatcca tcagcaagaa actgcagaag
    45961 aaaaagcttc aggagtctcc atagcttcag ggcactctcc ttctcataga caataagcgt
    46021 aaatgaaaaa cactgcttga acagttgtca cagactgtcc ttgcagtgtt tttgtttagg
    46081 cctgtttttt caagaactca gataagagga ataatccctt ttccaaggtt tcttttttac
    46141 aacagtaacc tagtcgggca tgcttcggta aatcaaaggc acttcctggt actaatagta
    46201 ctcctgtttc ttctaataaa ttgatacaaa atgtctggtc atcttcttcg atggctaatt
    46261 taatgaaaga agtagagacg taattgggag cgacaagttc aactttcggt tcattggcta
    46321 cccattgttt taaaatcaca aggttctctg taatgatctt tttgtttcgc gccaatattt
    46381 tttctttatt cttcaatgca tgtacagcaa gatcatctga caggactccg ccgcaaatca
    46441 tcgtgtagtc tcgatacttt cgaaaaactt ctgccaactc aggtccagta gctgtccagc
    46501 caatacgaat acctggaatg gagtaggttt tggataggga attggttgct ataccttttt
    46561 catagacatc tacgatcgac aagaattcac ctttatccgt taaaggcgca tatacttcat
    46621 ctatcaatac atatgcatct actgtacgag caatttctgc gattttttct aaagttttcc
    46681 gatctagcaa tgtgcctgta ggattgtttg cactattcaa acagatgatt tttgtttcag
    46741 gtttgactaa ggcttctagc tgttctaaat caaactgcca atcttcttct tccttcaaat
    46801 gaacaaagtc aacagcggca cctaaagatt tggggatgtc atataattgc tgataagtag
    46861 gaagcatcga aacaacatgg tctcctgctt ccactaacgc gtatagagct aaaagattag
    46921 caccagtcgc tccattcgtt tgtaagatgt tttccggatc gacggtttga taaagagaag
    46981 cgacttcttg tttgaaaaca tctgatcctt cgatccagcc atagtccaat ggttcgtttt
    47041 tgcgtttttc gaaaaattta cttacgctag tcccatctaa gccaacgagt tcgtctaatg
    47101 tcaatgcttc gatcgagctt tgtgcaatat catataccgc tttcttttcc catttattta
    47161 gccattcttc tacaccgaaa tttgcgattt tcatgtttct ttccccctta tatcttgatc
    47221 cagcctacag caagcaaccc gattgaaagc agtgcggcca cacaaatcag cccaatcatt
    47281 actttttctt ttcccgtaat ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa
    47341 aaaccaggaa gataggcaat actacataaa aggacataag aaaatcctgc taaaatgatg
    47401 cccatcaatt caaaaactac agcaatccca ccgatcacta cttgtttttt gattccttct
    47461 tttaaagaaa actggacttg atagatcccg acaagtagat aacaaatcaa aattgccgct
    47521 gtacataaag aataggcaaa ttgataggca taatcagtaa ctaagaaagt caataagaat
    47581 agattagtca agattgcagt aacaactaac gaataagttg gggcattttt tttattcgtt
    47641 tttccccaac ctttcggcaa cagaccgtct cgagccatca acatcgttgt ttctgctggg
    47701 agcatcgtcc aagacaacca agaaccaatg atcgaaataa tcaagccgat attgatcagc
    47761 gctgctcccc aatttcccac gattcctttt aaaatatttg ccatagcagg ttgtgaaata
    47821 gttgctaact cttcttgaga taagacgccg tatggcaaga tcgaagcaag gatatagata
    47881 aacaataaac aaagcagacc aatgatcgat gcttgctggg cttctgaacg tttcttcgcg
    47941 cggttggcta aaacacttgc gccttcgatc cctacaaaga cccacatcat aaccatcaaa
    48001 caacctttaa tctgcaacca gatcgttcct tgctgccctg ttccattcac tagattatca
    48061 gatacgttac cccaaaaatc agcagtgaat atattcactt taaatgcgat aaatacaaat
    48121 attaaaaata ggaataatgg cactaattta cagatcgtca cgattgtatt gatgacgctc
    48181 gctccttcaa ctccattgtt tactaataaa gtcagacccc aaataaaaat acttgcaaat
    48241 aaaatagaag gaacattttg tccacctttg aatatcggga aaaattctcc catggcgctc
    48301 atcagcatcg ttgcaaaagc aacatttccc agccacgcag acaaccagta accccaacca
    48361 ctgatgaagc cacctaattt accaaatgaa gcttccgcgt aaccgaaaat accaccatct
    48421 agatctggac gcttttctcc taaatgattc agcgataata ccaaagctaa aataccaatc
    48481 ccaacgatta tccatgaaag gatcgctggc cctggagctg cagaactagc cagatcactt
    48541 gtgatcccaa acacaccgct tccgattgac gaactgacaa ccagagcaac cagtccccat
    48601 ttgctaatac ccgtcgattt cttttccatt tccatcaccc tcacttataa aattgaacaa
    48661 atcacttgca aaagattccc ttctattttg caactgctct tgcttaacaa aaagcaactt
    48721 tattcatatt attggatatt atgaac
//
LOCUS       DACCWK010000012        47524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCWK010000012 DACCWK010000000
VERSION     DACCWK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.012"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(110..844)
                     /locus_tag="IVW65_000601"
     CDS             complement(110..844)
                     /locus_tag="IVW65_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7899524.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1171..1581
                     /locus_tag="IVW65_000602"
     CDS             1171..1581
                     /locus_tag="IVW65_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7899525.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1574..2275
                     /locus_tag="IVW65_000603"
     CDS             1574..2275
                     /locus_tag="IVW65_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7899526.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2275..3861
                     /locus_tag="IVW65_000604"
     CDS             2275..3861
                     /locus_tag="IVW65_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7899527.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3880..4101
                     /locus_tag="IVW65_000605"
     CDS             3880..4101
                     /locus_tag="IVW65_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7899528.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4098..4859
                     /locus_tag="IVW65_000606"
     CDS             4098..4859
                     /locus_tag="IVW65_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7899529.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5239..5748
                     /locus_tag="IVW65_000607"
     CDS             5239..5748
                     /locus_tag="IVW65_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7899530.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5823..6362)
                     /locus_tag="IVW65_000608"
     CDS             complement(5823..6362)
                     /locus_tag="IVW65_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7899531.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6502..7113)
                     /gene="rpsD"
                     /locus_tag="IVW65_000609"
     CDS             complement(6502..7113)
                     /gene="rpsD"
                     /locus_tag="IVW65_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7899532.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7429..8226
                     /locus_tag="IVW65_000610"
     CDS             7429..8226
                     /locus_tag="IVW65_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7899533.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8254..8889
                     /locus_tag="IVW65_000611"
     CDS             8254..8889
                     /locus_tag="IVW65_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7899534.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8909..9682
                     /locus_tag="IVW65_000612"
     CDS             8909..9682
                     /locus_tag="IVW65_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7899535.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10090..10887
                     /locus_tag="IVW65_000613"
     CDS             10090..10887
                     /locus_tag="IVW65_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7899536.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10865..11278
                     /locus_tag="IVW65_000614"
     CDS             10865..11278
                     /locus_tag="IVW65_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899537.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11262..13907
                     /gene="mutS"
                     /locus_tag="IVW65_000615"
     CDS             11262..13907
                     /gene="mutS"
                     /locus_tag="IVW65_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7899538.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13925..16033
                     /gene="mutL"
                     /locus_tag="IVW65_000616"
     CDS             13925..16033
                     /gene="mutL"
                     /locus_tag="IVW65_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7899539.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16055..16615
                     /locus_tag="IVW65_000617"
     CDS             16055..16615
                     /locus_tag="IVW65_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7899540.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16700..18688)
                     /gene="treP"
                     /locus_tag="IVW65_000618"
     CDS             complement(16700..18688)
                     /gene="treP"
                     /locus_tag="IVW65_000618"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7899541.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18911..19624
                     /gene="treR"
                     /locus_tag="IVW65_000619"
     CDS             18911..19624
                     /gene="treR"
                     /locus_tag="IVW65_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7899542.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19701..20147
                     /gene="msrB"
                     /locus_tag="IVW65_000620"
     CDS             19701..20147
                     /gene="msrB"
                     /locus_tag="IVW65_000620"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7899543.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20317..20919
                     /gene="ruvA"
                     /locus_tag="IVW65_000621"
     CDS             20317..20919
                     /gene="ruvA"
                     /locus_tag="IVW65_000621"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7899544.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20932..21933
                     /gene="ruvB"
                     /locus_tag="IVW65_000622"
     CDS             20932..21933
                     /gene="ruvB"
                     /locus_tag="IVW65_000622"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7899545.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21962..22540
                     /locus_tag="IVW65_000623"
     CDS             21962..22540
                     /locus_tag="IVW65_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7899546.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22592..23074
                     /locus_tag="IVW65_000624"
     CDS             22592..23074
                     /locus_tag="IVW65_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7899547.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23191..23565
                     /locus_tag="IVW65_000625"
     CDS             23191..23565
                     /locus_tag="IVW65_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899548.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24243..25595)
                     /gene="brnQ"
                     /locus_tag="IVW65_000626"
     CDS             complement(24243..25595)
                     /gene="brnQ"
                     /locus_tag="IVW65_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7899549.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25742..26332
                     /locus_tag="IVW65_000627"
     CDS             25742..26332
                     /locus_tag="IVW65_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7899550.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26460..27809)
                     /locus_tag="IVW65_000628"
     CDS             complement(26460..27809)
                     /locus_tag="IVW65_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7899551.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27977..28717)
                     /locus_tag="IVW65_000629"
     CDS             complement(27977..28717)
                     /locus_tag="IVW65_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7899552.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28730..30322)
                     /locus_tag="IVW65_000630"
     CDS             complement(28730..30322)
                     /locus_tag="IVW65_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7899553.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30890..31186)
                     /locus_tag="IVW65_000631"
     CDS             complement(30890..31186)
                     /locus_tag="IVW65_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899554.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31362..32087
                     /locus_tag="IVW65_000632"
     CDS             31362..32087
                     /locus_tag="IVW65_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7899555.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32080..32922
                     /locus_tag="IVW65_000633"
     CDS             32080..32922
                     /locus_tag="IVW65_000633"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7899556.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32925..33446
                     /locus_tag="IVW65_000634"
     CDS             32925..33446
                     /locus_tag="IVW65_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7899557.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33699..34262)
                     /locus_tag="IVW65_000635"
     CDS             complement(33699..34262)
                     /locus_tag="IVW65_000635"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7899558.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34513..34782
                     /gene="rpsO"
                     /locus_tag="IVW65_000636"
     CDS             34513..34782
                     /gene="rpsO"
                     /locus_tag="IVW65_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7899559.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34970..37096
                     /gene="pnp"
                     /locus_tag="IVW65_000637"
     CDS             34970..37096
                     /gene="pnp"
                     /locus_tag="IVW65_000637"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7899560.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37606..37872
                     /locus_tag="IVW65_000638"
     CDS             37606..37872
                     /locus_tag="IVW65_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7899561.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            38016..40007
                     /locus_tag="IVW65_000639"
     CDS             38016..40007
                     /locus_tag="IVW65_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7899562.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40361..41662
                     /locus_tag="IVW65_000640"
     CDS             40361..41662
                     /locus_tag="IVW65_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7899563.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41792..43162)
                     /locus_tag="IVW65_000641"
     CDS             complement(41792..43162)
                     /locus_tag="IVW65_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7899564.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43344..43667)
                     /locus_tag="IVW65_000642"
     CDS             complement(43344..43667)
                     /locus_tag="IVW65_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7899565.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43992..44600)
                     /locus_tag="IVW65_000643"
     CDS             complement(43992..44600)
                     /locus_tag="IVW65_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7899566.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44930..45646
                     /locus_tag="IVW65_000644"
     CDS             44930..45646
                     /locus_tag="IVW65_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7899567.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45659..46354
                     /locus_tag="IVW65_000645"
     CDS             45659..46354
                     /locus_tag="IVW65_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7899568.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46438..47067
                     /gene="udk"
                     /locus_tag="IVW65_000646"
     CDS             46438..47067
                     /gene="udk"
                     /locus_tag="IVW65_000646"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7899569.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15408 a         8795 c         9888 g        13433 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaaataaa
       61 aaaacttggt acgcaatcgc tgtaccaagt ttttttattt agcttttgat taagccataa
      121 ataataattt atataagaat actgctacga caccagcaca gataggtgct acaactggta
      181 cccatgcata tccccattga gaagaacctt tatgtttcaa tggaagaagt gcgtgtacaa
      241 gacgtggagc aaagtcgcgg gctgggttta gtccaggacc tgtaggacca ccaagtgaag
      301 caactaatgt ccatacaagg aaaccaagtg ctaagtgagc tgtaccaaga ttatcttgga
      361 agaatggtgc ttttgtaata cctagggcac caaagaataa aacgaatgaa ccaaagaatt
      421 cattgacgaa accattgaat ttagaaccaa cgctgttgat tgttgagaat gtacctaaca
      481 catggtttac gtcttctgtt tggtcgtagt aaggtttgta acaagctact acgatcaatt
      541 gtccaatcgc tgcaccaatc aattgtgcaa tgatataagg aacaacttca gcccaaggga
      601 atagaccatt agatgctaaa gcaattgtaa atgctgggtt gatgtggtta ccgctgattc
      661 caccaaaaat cattgctggc atcataaccc cgaatccata cccgacagca atcagcatcc
      721 agtcactgcc atatcctttt gtacctttta aatctacgtt tgctacagca ccgttaccta
      781 gaacgattag taatgctgta ccaatcattt ccgccgcaag acgggtcatt agtgagtaat
      841 ccataaatac ctccaaaagt tataaaaatg caaaaaaaaa agaaacatca acacgaaaag
      901 catcggtgca gtgtttcgtg tcggaaggat ttctgcagtc ttaacaaatg tgaataaaaa
      961 aatgaattcc attacttata agaaaccaga ccaacagtaa aactccaact ctttttaaaa
     1021 gttctccaca aatcaccaag cttaactata aatataccaa aaaaaaaacg ttttttttct
     1081 caaaaattgt atcttagaaa cagcatgttt tgaaactttc agaaaaggtt tgttagtaaa
     1141 atgaaagcta tacgtaagga gtgttgtttg atgaaatttt ttcaagagtt tttcattatt
     1201 attttctttt ctgtattagg agaaggcata cggatttttg ctcatttacc tattcctgga
     1261 agtatcttag gtattctttt tttattttta gcatttgaaa tgcatcttgt tgatcctgaa
     1321 aaaatcggag caactggtga ttttttattg aataatctag ccattttatt tgttcctgct
     1381 ggtgtaggtt tattggaata ttttgatgat attgcttcga tatggcctgt tttactggga
     1441 gcagtcgtcg tgtgttccat cgtcacgatg gttgcagcgg gaaaaacggc agaaggtgta
     1501 gaagctttgc tgggctatgt ccgtaagaaa aaagcagaaa gggtatctgt aaacgaaatg
     1561 gaggagaatc aaattgactg agttttttgt gaatccatta tttggcttaa gtttgacttt
     1621 aggagtgtac gtattatctg acaaattttt gcggaaatat aaactgcctg ttttgaatcc
     1681 tctcgtatta tcgattgttg ttattatctt atttttgcat ttttctggga tcagctataa
     1741 agactataat atcgggggca attttttgac tatgctgatt actcctgcga cagtggcact
     1801 tgccatccct ctttataaaa acttccgttt actgaaagag aatttttatc ctgtaatggc
     1861 tgcgatttta gcaggaatag tcgcaaattg cttggttagt gtagggttcg gttacttctt
     1921 ctctcttcat aaagagatgg tgatctcatt actacctaaa tctgtaacga cggctatttc
     1981 tgtcgattta tctcatacga tgggcggggt gaatgccgtg acacttgcta ttgttgtctc
     2041 tacaggtatt tttggttcgc tgatcgccac tcatattttc cgcctattca agatcgaaag
     2101 tccagttgct cgcggtgtgg cgttaggaag tacttctcat gctattggta cagcaaaagc
     2161 aatcgagatt ggtgaaatag aaggaatcat ttccggactt gcgatatgcg tcaacggaat
     2221 attgaccgtt ttattgctac cgttattctt tcagcctttt gctggcttgt tttgatgcga
     2281 aaaccactat tttatgctac aataaccaaa acgttcgagc ataaaaaagg agggaaagac
     2341 gtgtatttgt cttccgagta tcttaaacaa tttgataaag atctatatta taagattctc
     2401 ctcctagaaa gttttgagga tcaagcttgg cataccgctg cacaattagc gcaagttgtt
     2461 cagctggatg cccgcagcgt gtcgaaatac ctgaacgagc tgtctaaaaa ctatcagcag
     2521 tttagcggta agacacaccc tttgtttaca aaaaatcacc gttctggtta taacttttat
     2581 gatacgctcg attcaattga acatgagcgt tttttgatct atctcgtgca aagtacgctg
     2641 aaattccagt tgctgcacga catttttttt gaagaattcc atacaatgta ccaatttgcg
     2701 caaaagcatt acattagtga atctactgct catcgaaaaa tcaatgaatg gaaacagcaa
     2761 ctgcaaactt acggaattcg gctgcaacga ggtacatata tcgctcaagg cgaagaagaa
     2821 atcattcgac tttacttgca tatgacattc tggcagttgt ttcgagggaa aatctggcca
     2881 tttgaaacga tttctcaaat ggacgtaaag aatatggcag aacatatcat ggcttttttt
     2941 aatgttcgtc tgaacgagat caaaaaaaga cgattggaat atatgctagg tgcctttttt
     3001 ttaagaaaat cacagaagca ttatgttgta ttgaatgaga aaaaaagacg gctgatttct
     3061 gacaatctgt tgttccagcg attttgccag gtcatggagc cggtatttcc taattatttt
     3121 caagtagaag acgagttagg cgcattattt cttgtactga tgactcgtga agaatattat
     3181 agtgatccta agatacggaa gaaaattttt gattttcatc aacaagcaaa aacaccgccc
     3241 tttacagccc tttcagaagc aaaagccgct ctttctcttt atcaggaaga gcaggggctg
     3301 ccagcagaaa atctaacctt cgaggcagaa aactatttat tcagcagtca ttttttcgcc
     3361 tatctttttc ctaatgcgaa agaaacgatt gatggcaata gtagtgattt tattaatcat
     3421 ttagttattg aaaacaagga attgaaacaa tggctcgtac atttttttga aagtagacac
     3481 aaacatccca atcatctggc atttaaaaat catgcgtttt tgatggggcg gtatctgact
     3541 gtgtttaaaa cattaggagc ctttactcca cagcttccaa agatcacgat ccttttaatg
     3601 acagatttcc cattatttga agagcaactg ttagaggagg gattacggac atttttccgt
     3661 aatgagtatc agctgatttt tctgccaaca gattatcggg ggagagaagt tgatcttctg
     3721 atttcgacaa gtaaagtcca ccgcaaacca tgggcggatc ttgactattt tattgtgact
     3781 gaagaactaa aattaattga ttacatccaa ctatctcaaa aatttgaaat gattcaaaaa
     3841 caaaaactat taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta
     3901 ccagaattga aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat
     3961 gttgttctcg tatccgatga tgaatttgat ggctgtccgg tgacgccgta cttattgatc
     4021 aaaacaagtg acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat
     4081 gattgggcga tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta
     4141 tcacaggagc tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg
     4201 cttttgtatt tggattagat caaaagttcg gtaaaatgga agaaatttct ttgcgatttc
     4261 ctgaaacttt tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg
     4321 tatcgaaatg ccaaaatcat tttggtgaag ttacgatttt actgaataca gcggggatat
     4381 tggatgccta caaacccttg atggaaactg acgaatcgct gtgggatctg atttatgaga
     4441 cgaatgtgaa aagcatgtat cagctgacaa aaatcatcct tcctgatatg atccgccaaa
     4501 aaagcggaac gatcatcaat atggcttcga ttgctggctt gattgctggt ggaggtggga
     4561 tcgcttatac aagcgcgaaa catgcgattg tcggatttac caaacaactc gctctggatt
     4621 acgcaggaca gggtatccga gtaaaaggaa ttgctccagg agccatccaa acaccaatga
     4681 atgcggctga ttttgaaggg aaaggtgaga tggcagagtg ggtggcaaat gaaacaccag
     4741 tcaagcgatg ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc
     4801 aagcaagcta catacaagga acgatcatcc caatcgacgg cggatggttg ttaaaataac
     4861 cttgtgtttt tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat
     4921 aaaaaaagtc gatgactgtt cccgacattc gatgccgaac agccagttca aaaaattctt
     4981 tttaataatc gttaatattc gaaactaagc ggcttttcac gacctcttga tataaacgat
     5041 attcaaaagc ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata aggagctatt
     5101 tttatttgac cattcttatt acttgttgca gggcagcttt ttcactacct tctgcgacac
     5161 ttcagttgga attttcttcc catcctttgg tcgcggtcat taccaagata aaattatctt
     5221 gcaaaggaga aatagaacat ggaaaaaaat aatacccgtt cagccaaacg ctggacaacg
     5281 acagatgtga ctcggatggc gctagtcact ggtttatatg tggcagtgac gctggttttg
     5341 tctgtcttta gctttggagt gattcagatt cgtctctcgg agatgttcaa ctatctgtcc
     5401 ttgtataaca aaagatacat cgtcgcggta acagcgggag tagctttggc taatattgct
     5461 tctcctcttg gactgataga tgttgcagta ggaagcgtca gcacgctgct tgtattgctt
     5521 atcaactata aaatcactag tcgcatcaaa aacatgaaaa tcaaaatggc cgtgacagca
     5581 tgcgtatttg ctttttccat gtttacagta gctggtcagc taacgatttt atatcaacta
     5641 ccttttttct tgaattggtg ggtgattgct ttaggagaat tggcatcaat gattgtcggt
     5701 gggataatta tttattggat cggtaaaaaa gtggatttgt caaaataaag atctgtttaa
     5761 tttaaaatag ggcagcatgg aagagactga atgttgagtc ttcttccatg ctgctttttt
     5821 gattaatttg tatgaagaaa tcgatttagc cgttttttca tcaagatacc gatataagct
     5881 gctccagttg ccttgattgc ttccggcagt aaaaacggca gtagtccact ggcaaatgca
     5941 gttgtaaaag taagatcacc acttattttc agccagatcg ttccgcagat caatgcagca
     6001 agagagccca ttagattggc aaagattgca tataagtaat gagcatccgt tctttcgatc
     6061 atccaaccag ttatccacgt ttgtaagaga aatccaatca aatatccgcc agttggccca
     6121 aataaaacag ctatacctcc agtcatccct gaaaatacag gaagaccaat caagccaagt
     6181 aaaagataaa ctccaacaga tatcaatcct agttttcttc cgagaaaagt tgctgttaat
     6241 ccaatagcaa atgtttgccc tgtcaaagga accaaaccaa aaggaatggt catctgagaa
     6301 aggacagaaa taatcgcggc aaattccgca gcaagtatca attctttcaa tgttcggttc
     6361 ataacgccct cttttcgtta acttaatatt tttatttggt taacgaaatt gtaaaacaaa
     6421 gtgattactt tgtaaataga aaatataaaa aaagaccagc tatccaatag atagcggtct
     6481 ttttcaagaa agcttttgaa attataagct ttggttgtag tattcaacga caagagcttc
     6541 gtcgatttct ggatatagtt cgtcacgttc tggtaaacga gtcaatgaac cttccaattt
     6601 ttcagcgtca aagcttacga atgctgggcg tccaacagtt gcttctacag cttcttttac
     6661 tgttacgatg ttttgtgatt tttcacgaac tgagatcact tgacccactt ctacgtggta
     6721 tgaagggata tcaacgcgtt tgccatcaac taaaatgtga ccatggttaa ctaattgacg
     6781 tgcttgacga cgagtcgttg caagtcctaa acggtaaaca acattgtcta aacgttgttc
     6841 tagtaagatc atgaagttaa caccatgttt accttctttg attttgctag cttttacgaa
     6901 caagttacgg aattgacgtt cgttcattcc gtacatgtga cgaagttttt gtttttcagt
     6961 taattgcatg ccgtattctg attgtttgcc acggctatta ggtccatgtt gacctggtgc
     7021 gtaagggcga cgtgctaatt cttttcctgt gcctgacaat gagatgccta agcgacgaga
     7081 aattttccat gatggtcctg tataacgaga cattaaaatt tcctccaatg aatataagtt
     7141 tttggagtaa aataatccgt tgaaaaattc ataatcatat agtttgttct tcaatcttca
     7201 cctttgcagc cgcggttaca caatcgaacc cgagacgggg caacaaacta gggatgagtt
     7261 attatttttc tgctgcatta ttttacacaa attacagtat atgatagatc atccacgaat
     7321 gtcaagataa ttagtacata aaaacgcttt acaaacctag gctgtcttta taaaatgaga
     7381 gggtatgtgc aaatggaaaa aagcgaagga agtttaagga gaaaatcaat gaatccggta
     7441 tcaccattat ttgagaaaat cgatgcttca attgaaaaga aggcgaatct ttttgaaaac
     7501 agcttttcac gttacgctgt tcgagcggtt ctcgcctgtt tgtttttgac tttaggaacg
     7561 gctgtggcat ttgcgatcgc gatgaagggg gaagatatcg cgcatggtct aggaaagatt
     7621 ttatacgcat ttatgttcag ctggggtctg gtcatgattc tatacatgaa cgccgaatta
     7681 ggaacatcga atatgcttta tatgaccgtg ggtgtttttc ggcagagact tgctttaagt
     7741 aaggcagcca agattctatt tgcctgtatt tttttcaata tgattggcgg tatattatgt
     7801 ggatttctaa tttctatgac tacgccattt caagatttaa cgttggacaa ctttatgtta
     7861 gaatcaatag cagtaaaact agcaaaacct tcagcaacga ttttggtgga agggatgttt
     7921 gcgaatatcg ttgtcaatac cgctgtactg atcagcatgc ggatgaaaga ggatgtcgga
     7981 aaagtgatca cagtcgtatt catcattttc atttttgctt ttttaggata tgaacacgtg
     8041 atagcgaatt ttcctgcctt tacgcttact tactttgctt ctcacggaca gatggatgga
     8101 atgacagtca gcaatgtgtt acataatttg ttttttgctt tggcaggaaa ttatattggt
     8161 ggcggtttag taatgggact gggatatgct tggctgaatc agtcaaaatc atcctatgtg
     8221 gactaaaaag tcgttcatta aaggggaagt gccatggaag ttttagtttt tccaaaaaat
     8281 gaggaaaaag cgttacaaat ggaaaagtac atgaaaaatc ttttttcctt tgctggagta
     8341 cctaagccgg aacgcgctct tttacaacaa ccgttactgg atgagagcaa aaatttatct
     8401 atttctgaag ttttagcttt tgttcattat tattatgaac aaatcgaaag agagtatcag
     8461 tatgtagcga tcgatttagt ccagaaaaac atcaaacggc ttacatttga agacttagtc
     8521 gtattgttgc cactagttca gaaaaaagaa tggtgggaca gcatcgactc ttggagaaaa
     8581 gtatacagca catggtgtaa agcctaccca gagcagcgtg aagaagtctt tcaattgttt
     8641 gaaaaaaagg aagatttttg gctacgtcga atagcaatca cgttgcaatt aggttataaa
     8701 cagcaaacag acacaggact attgatccga gcaatagacg aagatcgtca aacaaaggaa
     8761 ttctttatcc aaaaagcaat tggttgggca ttacgcgatt atagtaagac cgatgccgat
     8821 tgggtaaaaa gacagctgac aacagatttg agtaagttag ctgttcgaga ggcaagtaag
     8881 tatttataag aaagcgagac gacaagagat gagacgcaaa ttatttgctt ttgatattga
     8941 tggaacgttg ttagattcaa acaaacaagc tttagatagc acaagagaag cattggaaaa
     9001 attacgcaaa gccggtcatc tggtcacgat tgctacaggg cggagccgat ttcatgcgca
     9061 agagatcatt cgcgatcttg attttacgaa ctacatttta tgtaatggag cggccggttt
     9121 tttagaccat gaacaagtat ataagaattt actagatcgc gaccagttgg atgcgttcgt
     9181 tcaagaagcg catcatgagc agatcgatac agcttttgtc agcttagaca atatcaagcg
     9241 ttcttcttcc aatgatgtaa cggtgatgga agaagcgatg cattctttcg gtgctgtctt
     9301 gcctgaatta gatcagtttt ttgcagagga acaagaaata taccaagcgt tagcattttt
     9361 cgatcaaaca tatgatggga agttctcttc atatgataaa attcgatttg tccgctggca
     9421 tgagaacagt gtagatgtcg tacctaaaaa tggttctaaa gcagcgacaa ttctgcactt
     9481 agcagaacaa gtaggcatcc ctcatgaaga catcatcagc tttggcgatg gacaaaacga
     9541 ccgtgaaatg ttgcgcatgt caggaattgg gatagctatg gggaatgctg tgccagaagt
     9601 acaagcagaa gctaagatgg tcactgatac aaacgatcag gatggaattt ggaaggcatt
     9661 gaaagagtta agcgtgattt aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa
     9721 gcccagccaa acaaaaatag ctcctcctat ttttcgtttt tctgggctta ttaccgcaga
     9781 tgcaagcttt cgaacaccgt ttaaggaaga ggtcgtgaca gaagtggtca gtttcaagaa
     9841 ataagaaggg atttacgaaa attgcttctc aaatttttgt aaaattacgg cttatttctg
     9901 aagaagtaga tgaaacaacg tttattcggg aaatgggact gtgacaagat gtttgtcata
     9961 gtcccatttt tttattttat ttttcaaaac taagaaatac caggaaagag ttccttttgt
    10021 ttgacctttt ctttcttatt gaaaacaact ataatataga gtgatgttaa aagagaagag
    10081 gaatttagtg tgcgggtatt gtttattggt gatgtagtag gttcaatggg acgagagatg
    10141 attactgaat atcttcctag attgaagaag aagtaccgtc cgcaagtaac gattgtaaat
    10201 ggtgagaatg ccgcttctgg acgtggaatc acagaaaaaa tctataaaaa atttttgcaa
    10261 gacggcgtag atgtcgtgac gatggggaat catacgtggg acaaccgcgg aatctttgag
    10321 tttgtaaatg aagctaaaaa gatggttcgt ccggcaaatt ttccggaagg gacgcctgga
    10381 caagggatgg tctttgtcaa agtgaaccaa gtcgaactgg cagtgatcaa catgcaggct
    10441 cgttctttta tggtggactt ggatgatcct tttcgtaaaa tgaatgagct ggtagaagaa
    10501 gccagaaaac gtacgccgat catttttgta gattttcatg gagaaacaac aagtgaaaaa
    10561 caagcaatgg gctggttttt agatgggaaa gtttcagcag tggtcggtac acatacacac
    10621 gttcagacaa atgatgcgcg aattttgcca agaggaacag cgtatctgac agacgttggg
    10681 atgacaggtc cctatgatgg tattttgggt atgcgcagag aacctgtaat cgaaaagttt
    10741 ctgactgcat taccaaaacg ttttgaagtt gtggaacaag gccgatcgat tttaagcggc
    10801 tgtatcttgg aattggatga tacgacaggt catgcaaaag aaatccagct gattcagatc
    10861 aacgatgatc gaccgttcat ggagtagaaa gagaggcagt tttttgcagc aggagccgga
    10921 aattcaaaaa gaagtaagga aactgacaaa acttttaaga gagaatgaaa cgatcattcg
    10981 ctacaaagaa cttgaagaaa agatccagca aaaccagtat ttagctgagt tgagagaaaa
    11041 aatcaaacaa gcgcaaaaag atgctgttca ttttgcccac tatgacaaac cagcagcaga
    11101 aaaagaagca atcaaacaag cggatcaatt catgcaagaa tttgatcagc atccgcttgt
    11161 tgtagcttat agaaaacagc tgttggaagc agatgatctg cttcatcatt taacaactat
    11221 gatacaagaa gaaatcaatg gacaaataga ggaggaaaaa catgcctcaa aaaactaaaa
    11281 atactcctat gatggaacaa tatctaagca tcaaggccca ataccaagat gcttttttgt
    11341 tctatcgttt aggagacttt tatgaactat tttacgaaga tgcgatcaaa gcgtcacagc
    11401 ttttggaatt aacgttgacg agcagaaatc gaaatgcaga agaacctatt cctatgtgcg
    11461 gtgtacctca ccatgcagca caaggatata tcgatgcctt gattgaaaaa ggctataaag
    11521 tagcgatttg cgagcaggta gaagatccaa agacgactaa agggatggtc aagcgcgaag
    11581 tgatccagct agtcactcct ggtacagtca tggacagcaa aggattgtcg gcaaaagaca
    11641 acaatttttt aacagctgta gttagtcaag gaaatgaatt tggctttgct tatgccgatt
    11701 taagtacggg agaattgaag acggctcggt tgcatgatga agaagctgtt ttaaatgaag
    11761 cctctgcatt gcagacaaaa gaactcgtat taggcagtga gatcactgat acccttcgtg
    11821 aacagctaag cgggcgttta gggctagtct tttctcaaca aaatgagatg gaagaaaacg
    11881 cggaatttag ctttttgaca agcgaactag tcgatccgct ggaaaaagaa gcaaccggca
    11941 aactattgac ttatttatcg gtgacacaaa agcggagttt agctcatatc caaaaagcag
    12001 tagaatacca accagatcat tttttgaaaa tggattatta ttcaaaattc aacctagaat
    12061 taagccaatc tattcggact gggaaaaagc acggcactct tctatggttg ttagatgaaa
    12121 caaaaacagc aatgggtggc cgtttactaa aacaatggct agaccgtccg cttatccagg
    12181 caaaacaaat taaagcaaga caagaaatgg tcgcttcttt gctagacagc tattttgaac
    12241 gtatcgattt gcagtcagca ttaacgaaag tctatgatct ggaacgccta gcaggtcgag
    12301 tagctttcgg taatgtcaat gggcgagact tgatccagct gaaaacttct ttagaacaag
    12361 tgccaatgat cagagggttg attgaaggaa tcgatcaagg tcaatggcaa agtctgcttt
    12421 cagaaatgca gccgatggac catctcgtcc aattgatcga ccaagctatc caagatgatc
    12481 cgccgttaca aataacggaa ggaaacatca tcaaagatgg attcaatgag cagctcgata
    12541 tgtaccgttc tgctatgaga aacggaaaga aatggttggc agaacttgaa gcaaaagagc
    12601 gtcaagaaac aggaatcaag aacttaaaaa tcggatttaa ccgcgttttc ggttattata
    12661 tagaaattac aaaagcaaat cttggaaatg cagaactgga gaagtatgaa cgaaaacaaa
    12721 ctttagcaaa tgcagagcgt ttcatcacac cagaactaaa agaattggag acacaaatcc
    12781 tcgaagcaga agaaaaatct gttgatttag aatatcagtt gtttttagct gtgagagaag
    12841 aagtcaaaaa ggctatccag ccgctacaag tacttgcaaa agcaatcagt gcagtagatg
    12901 tgctacaaag ttttgcaacg atcagcgaac gctatcagta tgttcggcca gaacttgtca
    12961 gtgacaagca tcagcttctg atcaaagacg gacgacatcc tgtagtagaa aaagtattag
    13021 gacatcagga atatatccct aacagcgtag aaatggcaga agacgaaatg atcttgctga
    13081 ttacaggacc gaatatgtct ggtaaaagta cgtatatgcg acagttggca ttaactgttc
    13141 tgatggcgca aatgggctgt ttcgttccgg caaaacaagc agttttacct atttttgatc
    13201 ggatttttac ccgaattggt gcaagtgacg acttaattgc tggacaaagt acttttatgg
    13261 tagagatgat ggaagccaat caagccctgc gttatgctac gccaaacagt ttgattttgt
    13321 ttgacgaact agggcgagga acagcaacct atgacggaat ggcgttggcc caagcaatca
    13381 tcgaatatat ccatcgtcat gtacaagcaa aaacactctt ttctactcat tatcatgaac
    13441 tgacggtact tgagaaagaa cttccgcagc ttaaaaatgt ccatgttgga gccgttgaaa
    13501 aagacggaga agttgtcttc ttgcataaaa tgatggatgg acctgcggat aaaagctacg
    13561 gcatacatgt ggcaaagatc gcaggattgc cttccaatct actagaacga gcagcagata
    13621 tcctgcatac gctagaaaat ggcgataacg gacaccatac tgttccagct gaagtagaag
    13681 agaaaactaa aacagccaaa caggagcaaa taaaaccacc tgcagaaaaa gtaaatcaag
    13741 accatataca agaccgtgta aaagaagaaa accaacagct ttctttattc agtgaattat
    13801 ccgaaaatga aacagaagtc attgaatcac tgaagcaaat aaatttacta gaaatgacgc
    13861 caatggatgc actaaacaca ttatatgaat tacaaaaaca actgtaggaa gaaggcggat
    13921 atcgatggcc aaaatccaag aattatctga acgtttagct aatcagatcg ctgcgggaga
    13981 agtagtggag cgaccagctt ccgttgtgaa agaattagtg gaaaatgcaa ttgacgccgg
    14041 cagtactcag attgatatct tagttgaaga agcgggactg cggacgatcc aagtgattga
    14101 taacggagaa ggcattttgg cagacgacgt agagaatgcc ttcaagcggc atgcgacaag
    14161 taagatccat aatcgcgacg atctttttcg catacgaacg ctgggctttc gaggggaagc
    14221 tttgccaagt attgcctctg tttctgaaat gactctggaa acggcgaatc aggaagaaaa
    14281 acaaggcacc tatctgtcgc tgaaaggcgg agaagttatt gagcatcgac cagcaccact
    14341 gcgtcaagga acgaagatta ccgtcagcaa tctgtttttc aatacgccag cccgcttgaa
    14401 atacgtgaaa acactgcaca cagaattagc gaacatcggg gacatcgtca atcgactggc
    14461 attgagtcat ccatcgattg cttttcgttt agtacatgat ggaaacaaaa tgctggcaac
    14521 tgctggaaat ggcgatctga aacaaacaat tgcgggaatc tacggattat ctaccgcaaa
    14581 aaaaatgctg aaaatcgaag gaaaagatct agattttgag ttatttggct atgtttcatt
    14641 gcctgaggtg actcgtgcca gtaggaatta cttatcgacg atcatcaatg gacgttttat
    14701 caagaacttt tccttgaata aagcaatcgt tgcaggttat gggtcaaaat taatggtcgg
    14761 tcgtttccct attgctgtat tggaaatcaa aatggacccg cttttagtag atgtaaatgt
    14821 tcatccgacg aagcaggaag tccggctgtc gaaggaaaaa gaactaacag agctaattag
    14881 taacgccatt caaaaagcat tgtctcaaga aaatctgatt ccaagtgcag cggataatct
    14941 acggtttaag aaaaaagtac ctgatcagcc taaaacagaa caaatcgaaa tcgatttgtc
    15001 ctacgctttt gaagaagaaa aacctaaaaa gccaagtagt ttgaattttg atcgagagac
    15061 aggtaccttt ttcgtagaag aactaaagaa cacatttgtt gacagaattg ttgataagtc
    15121 tgtggaaaat gttgaaaact attctggttt ttctgaagaa aacaccactt atccagaaaa
    15181 aacagcgaaa acggtggata agtttggtga aaatgttgat aactttgtag aaaaaacagt
    15241 ggaaaaatct gaaaagaaag aatttcccat tgtggataac atggagaaaa caaatgaaga
    15301 acaagcagat aaatccgtag aagaggatga aaagatttac gaggaagaat tgacactcca
    15361 tcctgaattt gatttcactc aaggaaacga caaaacatta gaacaagcca tcaagaaatt
    15421 agaaacggaa aaaccagtac aacgttttcc tcagttggaa tattttggac agatgcacgg
    15481 aacgtatctt tttgcccaaa gtagcgatgg gttgtacatc attgatcagc atgctgccca
    15541 agagcgcatt aagtatgaat atttccggga aaaaattggg gaagtatctg atgatctgca
    15601 agaattgctt gttccatttg tgttagacta tccaaacagt gatgctttga aattaaaaga
    15661 gcaaaaacac ttgctagaag atgcagggat ttatttagaa gaatttggtc aaaacagctt
    15721 tattgtacgg gcccatccta cttggtatcc caatggagaa gaagaatcga ttattcggga
    15781 gatgatcgat atgctgctga caactggaag tgtgagtgta aaaaaattcc gtgaagcaac
    15841 agcaatcatg atgagttgca aacgatcgat taaagccaac cattatttga atgaagcaca
    15901 agcacgtgtg ctgctgaagg atctggcacg atgcgaaaat cctttcaatt gtccccatgg
    15961 ccgtccagta ttgattcact ttaccaattc ggatatggaa aggatgttca aacgaatcca
    16021 agacccgcat taagtcaaag agaagaggga aaacatgaag attgtattag catcccaatc
    16081 accaagaaga aaagagttat tggctcatat cgtttcagaa ttcgagattg aacctgctga
    16141 tatcgatgaa acgccatttc cagacgaaca gcccgtcgat tatgttcgta gaatggctga
    16201 agaaaaagca cgaactgtct gggaacaaga tgaaaaagaa gaccatttag tgattgccag
    16261 tgacacgact gttgttctca atcaagagat catgggtaaa ccagaagatc tggctgaggc
    16321 tgaattcatg ctgaaaaaac taagcgggga aacacatact gtatatacag ctgttgtttt
    16381 aaaaacaaag gaacgagaag aacggatact agcagaagca catgtcactt tttatccttt
    16441 gacagacgaa gagatcaaac gctacttgga gacgggggat tatgcggata aggcaggggc
    16501 atacggcatc caaaatgctg cgagtgtatt cgtcaaagaa atttctggag attattacag
    16561 tatcgtagga tttccgattg gtgctgtcaa tcaggcactg aaaacattca tttaaacaaa
    16621 aaagcggact atctgatttt cgattgaaaa tccatgatag cccgtttttt ttattgaaaa
    16681 gattacttat ctactctgac taagatttta cttcagcttt accgactgct tcgcccgggc
    16741 gaacaagtcc tgattggtca agagaaagtc ctgcaacttt ttcattattg gtcaaagcaa
    16801 caataatgtc agttttcttg ccagcttgtt ctatttctgg caaatccatt tcagcaatca
    16861 gggtctctgg tgtgacaagg tatccttctt tcacgaatac attgaaagga acacccttta
    16921 gttcaacagt atctaatccc atgtgaacaa gtacttccag cccctcatta gaaagaatac
    16981 caatggcatg ttttgtttca aagacactcg tcactttacc gtttactggt gcatagactt
    17041 tgccgtttga cggttcgaca gcataaccgt cacccatcat tttttgtgag aaaactgggt
    17101 catttacttc agttatttcc ttgatggtcc ctgcagcgac tgcgaataat gtttcttttg
    17161 ttgcaactgc cgtaccagtt gctgaaacag gttcgaaaga ctgtggcgtt gctgttgcgc
    17221 catttgctgt ttgaagttgt acatcagcag cattttcagg aacagcagga tcaatcttat
    17281 tcaaaattcc ctgacgtcta aagatgatcg tcaagccaaa tggaatgatg acagcaatga
    17341 tcattgcgat gatgaaaggt acccatgttt ctgcttggat agctagtatt cctggcaaac
    17401 cgcctactcc gatcgcgtta gctcgaacac ccatcaagtt agcaaacatg ccagctaatc
    17461 cagagccgac cattgcagcg acgaaaggat agacgtattt caaattgatt ccgaacatag
    17521 caggttcagt tacgcctaag taacaagaaa tcattgctgg aatcgagatt tgttcttctt
    17581 ttttattccc acgatgtaag aagatgatgg ctaagactgc agagccttgg gcgatatttg
    17641 ataaagcaat catcggccat aaattcgttg atccgaaatc tgcaatcagc tgcatatcga
    17701 tggcattggt catatggtgg agaccagtaa tcactaaagg agcatacagg aatccgaata
    17761 cggcactaaa caaccagctg atagaagacg tcaatccagt atttacgata tttgagatcc
    17821 agcttccgac tgtccagccg ataggaccta aaataacgtg tgcagctaaa acagttggta
    17881 acaatgaaaa caatggaaca aaaatcatcg agatcgactg agggatatat ttgcggaaga
    17941 agatttctag atacgctaac ataaaccctg ccagcatggc tggaataact tgcgcttgat
    18001 aaccaatcat ctggacttga gcaaaaccaa aatcccaaaa tggaatatct gctgcggctg
    18061 tggaagctac actataagca tttaataatt gcggagaaac taacgtaatt cctaaaacga
    18121 ttcctaatat ttgtgtagta cccatctttt ttgcaatact ccaagtgata cctactggaa
    18181 gaaaatggaa aattgcttca ccgggtaacc acaaaaaggc attgacacca ttccaaaact
    18241 tcgagtgttc aacgattgtt ccaccaagac tttcaaactg gataccttca agtacatttc
    18301 tgaatccaag gatcaatcca ccgacaatga ttgcaggaat cagtggtgta aagatttctg
    18361 ctaaaacggc aatagcacgt tgaacgggat gaaggttttg ttttgcagct gcttttcctt
    18421 gttctttgga aaccccttca actccagata ctgcaacgaa gtcgttataa aaagtagaaa
    18481 catcgtttcc tatgatgact tggaactgtc ctgcattcgt gaacatccct tttacgctag
    18541 gtatatcttc gatcgctttt tcgtccgctt ttcctggatc attcaggaca aaacgcatac
    18601 gagtcgcaca atgactaact gcagcaatat tttcttttcc gccgatatca tgtaacagct
    18661 tctcggcatc tgcttgatat tttgccatat caataaaccc ctttctctta acttgtctat
    18721 gcaagttgtt tcttcattta ctatactttt tttcggagag aattgcaagc gttttattaa
    18781 aaagtacgag agaaaatgat atcgttttaa aaagcttaaa ataagctttt cttaaaaata
    18841 ggaacattga aatacaaaaa gagctgtagt atagtgaaga aatgatggaa gaacgagtga
    18901 gggaaaaaac atgaataagt tcaatgaaat ttttttagat ttggaacaga aaattttagc
    18961 gaaagaatat cctccgcatt cgcttttacc cagtgagaac caattgatca aaatttatgg
    19021 tgtttcacga gaaacaatta gaaaagcgct gaatcttttg actacggaag gttatattca
    19081 aaaaaaacaa ggaaaaggtt cgatcgtact ggatcggaat cgcttcgact ttcctatttc
    19141 tggattgact agctataaag agctacagga gacccaacgt attccaagtg aaacgatcat
    19201 ccatacgtta gaagaaacag aagtgacaaa agcattgaac gagatcactg gctgggagat
    19261 cggtgcgcct gtttggcatt tgatacgcga aagaaaaatc aatggcgaag tagttatatt
    19321 agacaccgat tatctattaa aggaaatcgt tcctatttta actccagcac aagcacaagg
    19381 atcgatctat gaatattttg aaaatgagct ttctttgacg attgcttacg cgcaaaagga
    19441 gatcacagta gaagaagtaa cggaaatcat gaaaacagcg atggatcttc acgatgacac
    19501 ttatgcagta gtagtcagaa gcctcgttca tctggaagat acccgctgtt tcgagtattc
    19561 tgagtcggtt caccgattag ataaattccg tttcgttgag tttgcccgac gccgaaaagt
    19621 gtaaatagtt caaaagaaag caaattgttg gcatctgtcc actttggcgc tatgatagga
    19681 tcacaaaggg ggcattttta atggataaac caataagacc agaaaaagaa gaactgaaag
    19741 aaaagttatc acccatggct tatgcagtca cacaagaaaa tgcaacagaa cgaccattta
    19801 gcgggaaata tgatgatttt tacgaaaagg ggatctatgt agatatcgtc agcggtgaac
    19861 cgttattcag ctctgcagaa aaatatgatg caggttgtgg ctggccatca ttcagcaaac
    19921 caataacgaa aagacaagtg cgcgaaaaag ccgatttttc tcatggcatg catcgaatag
    19981 aagttcgaag caaagaagca gattctcatc tcggacatgt gtttaccgat ggaccagtag
    20041 atcaaggtgg tctacgttac tgcatcaatt ctgcctcact gaaattcatt ccatatgaaa
    20101 agatggatga attaggttat ggggaattca aatcacttgt agaataatgg aaatacacaa
    20161 agagcggtac aatacgggga tttactcgat cgtccgctct ttttttattc aagcgattgc
    20221 tatttgaaag aaacgctccc tagactcctt ttttcacaag gtgaaatgtg ctacaataaa
    20281 gggaacgtat gtgtgcaaaa gaaagaggac tagaatatgt atgaatatct gacaggtgtg
    20341 gttaccttta tcaaccccta ttacatagta gtagaaacca atggtatcgg ctaccagatc
    20401 gcactaggaa atccttaccg ctacagtagc aaactaaatc aaaaaataaa aatttatgtc
    20461 catcaggtaa tacgtgaaga tgcgcatctt ctgtatggat ttgattcgct agaagaaaag
    20521 caactgtttt tgcgattagt aagtgtttca ggaatcggtc cgaaaagtgc ccttgcaatc
    20581 atggcaagcg aagaccactc cggtttgatt caagcagtag aatccagtga tgtcacttat
    20641 ttgacgaaat ttccaggagt agggaaaaag acagcacaac aaatgatctt ggatctaaaa
    20701 ggaaaattcg gtgagctgag tatcgataca ccttttagtc tatttgatga agcaaacaca
    20761 aaagacgcta cagctctatc tgaagcaatg gaagctctct cggctcttgg ttacagtgat
    20821 cgagaaatca aacgtgtaga aaaacaatta aaagaagtag ataatcagac aacggatgag
    20881 tatctacgcc aagcactcaa gctaatgatg aaaaaataag gaggagacca aatgacagat
    20941 gaatacttgt tatcaccaga atctggcgaa aatgaactga gtttggaaaa atctcttcga
    21001 ccacagctgc tttctcaata tatcggacag agaaaagtca aacaagaact tcagatttac
    21061 atcgaagctg ccagaaatcg ggaagaacct ttagatcatg ttctgttgta cgggcctcct
    21121 ggattaggaa aaacgacgat ggccatggtc attgctaatg agatgaccgt aaatattcgt
    21181 acgacgagtg gccctgctat cgaacgtccg ggtgacttag tagcaatctt gaatgaactg
    21241 gaagcagggg atgtcctgtt tatcgatgag atccatcgat tacctagagt ggtagaagag
    21301 atgctttatt cagctatgga agatttctat gtagatatca tggtaggaca agggccaacc
    21361 gcacatccgg ttcatttccc tcttcctccc tttactttga ttggcgcgac gacacgggca
    21421 ggtatgcttt ctgcaccttt acgagatcga tttggcatca tttctcatat ggaatattat
    21481 gacgaacagg atctgcagga gatcgtttta cgttcttcgg atatttttca aacagagatc
    21541 attgaagaag gggcattgga aattgctcgc cgatcacgag gaacgcctag aatcgctaat
    21601 cgcttgctca aacgggttcg tgattttgcg caagtccaag gagacgggaa aatcgatcgt
    21661 cctatagcgg atcaggcact tactttgcta caggtcgatc aggctggatt ggattatgtt
    21721 gaccagaaat tgttgcgaac aatgatcgag ttatatggcg gcggaccagt agggttaagt
    21781 acgctttcag taaacatagg agaagagcgt gagacggtag aagatatgta tgaaccgtat
    21841 ctgatccaaa aaggattttt aaagcgtaca cctagagggc gaattgctac tgcttatgcc
    21901 tatgaacatt ttggttacga ctatcatata tgatttttgc ttttagattg gaggaaaatg
    21961 aatgagtgat acaacacgta cgaaacaatt gatcaaaaac gtattgattg atttgtgcca
    22021 aacgcaatct tttcgaaaaa tcagcgtcca aaaaatcgca aacgaagcaa aaatcaatcg
    22081 acaaacattt tattatcatt ttacagataa atacgatttg cttcgttggg tctattgtga
    22141 ggatgcattg cattatttag atccacaatg tctttcactt gataattggg aagagcaagc
    22201 attgaaaatg ctgaaagcaa ttgctgagaa gaaagaattt tatgcaaata ctgtgctttc
    22261 tgacccagaa attttacaaa aagagttttc tcaactgatc caaaaatcat ttatcaagat
    22321 ttttgaacaa atggatgaag aaagacaact ttctgcaaga gacaaactat tttatgctcg
    22381 ttttttttct tacggttgca gtggtgtatt agtcaattgg atcacgaatg aatatgaaga
    22441 aacaccgatg gaaatcgcta tgcagctttt ccgtttagcc aaggatacgg aattatattc
    22501 ttatcgtctt tatgcaaaag aagagttgga agaacagtaa tagaaaaatt ttttgttgga
    22561 aaagcaataa tcgctaaaag gagatgggga aatgacaaaa attttatttg tttgtttggg
    22621 aaatatttgc cgttcaccga tggcagaagg cttgctgaga aaacgaatag cattagaagg
    22681 aagggaagca gaattttttg tggattctgc tgctacaagc acgtatgaag ttggaaagac
    22741 accgcaccct ggaacgaaaa aaatattaaa ccaagaaaaa gtagatatga cgaatatggt
    22801 tgctcggcaa atcacacccc acgattttga aacttttgat tggatcattg gcatggatca
    22861 agaaaatgta gaagaactga aacgaagagc accacgctca gcgcagggga aaatccattt
    22921 gtttttaagt agcattcctg gaaaagaaaa agaaaatgtt cccgacccat actataccaa
    22981 taactttgat gagacatatc acttgatcac agaaggcctg aatcattggt ggaatatttg
    23041 gattgatgaa aataaaataa caaacaattt gtaaagttaa cgctttcttt ttaaggcgat
    23101 gttagagcat ttccttgttt ttttgtaaaa tgttaggtaa aataaaatta attaagtaac
    23161 actttcttag ctaaaggagg agtgcattcg atgaacgaag agacagtatc gccaatgaag
    23221 aagcttacga agttagttac tcatacttcg tatcaacttt ttgattcttt tgtaaatgac
    23281 actgaagaac atgctaagaa tgtacgattg caaggaaagt ttattcttga gctgcaaaaa
    23341 gctgctactg aacatagtct agttatctta caagtaagag caaatcgttt gagtagcaag
    23401 tatgaaacga tctcaggctg ggtggcgaca aaaacggtcg aaaaagaccg agtgatgatc
    23461 cgcctacaaa acgaagagca gcagatccgg atggttcctg tagaacaaat tgaaaaaata
    23521 acaatccttt caccagcagg ggaacaggaa cggttatcaa aataatttat accagataga
    23581 aaagcacgta tgagcttgta ccatcaatca ttactaatca aaaataaccg aatgctttaa
    23641 gctgatatca gtaaaacgga acgttttagg atgagtatca cagcatgaca gcattgggtt
    23701 gattagttga atcgattgga tggtacaagt ttttttgata aaaaaaggtc ctgctagccg
    23761 gggagctagc aggataagga gttaaaaatg aaaaagtgtt gttagggttg tttgttggta
    23821 tgcttatata atagtcacta gttgtgaata ttttgtgacg aaagttttcg aaagttgtta
    23881 agtttttttg cgaaaactat ttttatatca tatatatgtc taaaatctat attataaaag
    23941 aaatgttgtt tgatcagctt ctcttcggta ttagttcaaa acaaaacggc aaagtatatg
    24001 gagctacttt gccgttttgt ttctctaata ctcaggtcag tttatagacg ctcaataaac
    24061 ggtgttcaaa agccagtttc tcggtattaa gtcaaattta tgcaaaaatg tgagaagcca
    24121 ttttcaaaaa atatttctta atactcgaaa ctgagcggct ttttcacgac ctctcaataa
    24181 acagtgttca agagacagcc tatcggcaat aaggctgaac gtctctttca caacctcttt
    24241 tatcaatctg ctacttcttc tttcttagcg aaactccaaa tcagtccgat aacaaaaccg
    24301 ataactgctg gtacgatcca ccccatcccc aatttgaaaa acggcaagta gctttccgca
    24361 acatataaaa gattttgaac aataggtgtt tgtttgatag tttctgggca agcgttcaac
    24421 ccatcaaaaa tcgaagcaat caatgtaaaa taagtagtca ttcgataaac agccgtacgc
    24481 tgcttaaaca aaggaccaac taacactagt aaaatcagtg tcattgctaa tggatagatg
    24541 aacatcaaaa caggcaacga aaattgaatg atattcgtca agcctacgtt tgccgctaaa
    24601 caaggaagga tactagcaag tgcaacaaag aatgcgtaat ttctttgcgg aaacaaatct
    24661 gtgaatgttt ctgcaaaagc tgtaatcaaa ccaataccgg tcttcaagca agctaatata
    24721 acgataaatg ctaataagat actgccataa gtacctaaat aatgatcagc aatctgagcc
    24781 agcgtgatcc cgccattttc acttagagca aagctaccta agctcatagc acctaaataa
    24841 gcgagcaaag tataaatgat tcccatcaat ataatgctga tcgcaccaga tttgatcgtg
    24901 tctttagcga tttcagcagg ttttgttaca cccatgtttt ggattgttgt gacaatgatg
    24961 atcccaaatg ctaaagcagc aagcgcatct aatgtgttat atccttgagt aaaccctgtg
    25021 aaaaaagcat gctcttgata atttggctga ataggcgctt gatttacaga acctagtgga
    25081 ttcaaaaaag caaggagaag caaagcacct aataaaacca aaaatgcagg attcaggaac
    25141 tttccaacat aatctaatat ctttgtcggt tttcttgaaa accaccaagc cagcacaaaa
    25201 aatacaatac tgaaaattgc tagatatagc gtctgtctgc taggatcgat aaatggtgct
    25261 aaaccaatct cgaaagatgt ggttgcaagg cgaggtaaag caaaaaacgg tccgataact
    25321 aagtaaagca agatggtaaa aaccatcgca tatgtttgat ttatccgttt tgctaattca
    25381 tatacacctg agcttttcga tacaccaatg gcaatcaccc ctaagaaggg aagaccgata
    25441 ccggtaacca agaaacctag attcgcccaa aaggtagcag cgcctgcttc ttgacccaaa
    25501 tgaacgggaa agataagatt tccagcacca aaaaatagac cgaaaagcat ggagcctata
    25561 aaaagatagt ctttaaatga taattttttt gtcatacata actcctttac ggttttttta
    25621 aaagtaacaa aatttcgaaa aatatgcaac aattttcaga aaatttccta tataaaacga
    25681 cggttattta ataacgtaag tgataaaata aaagcgctga atcatctgag gaggaatcat
    25741 cgtgacaaag tcgagattag aagcatttac tgatggagtg gtagccattg ttttgagagt
    25801 tcttgtatta gagatcaaga tccctaatac atctagcttt cattctttat gggtcatccg
    25861 aaatacatta ttagcttata ccatcagctt tatatttgtt gcggtgatct gggtgaacca
    25921 tcatcgtata tttcagatgg cagaacgaat taattaccga gtggtctggt cgaatatctt
    25981 tttgctcttt tggctgactt tatgtccttc tgttactagt tgggtcgggc gtaaccctga
    26041 agctttttgg cctgagatgg cttatgtctt agtatacacg atgtggagtt tttcttttgg
    26101 tatcttgatg aggcaaatca tgaaagcaaa taaaccagat tcccatgtcg tgagagtttt
    26161 gtcaaaggac cgtcgaagtc ttatctccat gatgattaac ttatgcttga ttgccggtgt
    26221 cttttttgta ccgttgatcg ggttattcgg acgttttttt gtatctggta tttggatttt
    26281 ttcctataaa aaagcagatg cctattatca gcggttgttt ccggggaaat agaaaagagg
    26341 ctgggacaga agcttttaac tccaagaaat aagccgaaat ttcacaaaaa tttaagaagc
    26401 aattttcgta aattccttct tatttctcga agctgaccac ttctgtcaca actactttct
    26461 tacttcttac gttcatattt catatgatag attagtaata gaagcaagaa ccacacaggt
    26521 gttagtaata aagcgattct cgtatctttt cctaggaaca agacgacaca cacaaaggcc
    26581 atgtaaagaa ataccagata attactaaat gggtacaacg gcattttaaa tgaatgagct
    26641 tttttgccga ggatactttt acgatatttc atgtgcgtgt agacaagcat actccagata
    26701 aacaagaaac aagtagtggc aatactgctt actagtgtga atacttcact aggcatcacg
    26761 taattcaaga taacagaaat gaaaatgacg atagtagaaa atgtcaaagc attccctggc
    26821 acatgatgtg ttgttaactt tttgaatttt ttaggggcac tgccttcttg cgctaaagaa
    26881 cgaagcatac gtcctgtact atatattgca ctgttgcagg cagaagctgc tgcacttaat
    26941 acgactaaat tgatcaagga agcagcaatc gtgatcccaa tttccgaaaa tacttctaca
    27001 aatggactgt tatctggatt caagctgttc catggataga ttgccataat gacaaaaaga
    27061 gatcctaagt aaaataagat gatcctgata ggaatattat tgatagcttc cggcaacact
    27121 ttttcaggat tttctgtttc tccagcgact aatccaacta attctatccc agcaaaagca
    27181 aaggttacca tttggaaagc taagataaat ccttttgcac cagttggaaa gaatccgcca
    27241 tgagaccata gattcgtgat actggctgtg ccaatatctg tcgtatagtg ggtgaggatc
    27301 atataagctc ctacgaggat aaaggcaatg atcgctacaa ctttgatcaa tgcaaaccaa
    27361 aactctaact caccaaacaa tgagacagcc accatattca agcaaagaag aagacctaat
    27421 gcaatcagtt caggaagcca ctgaggaaca ccaggcgccc agtagcgtac atacattccg
    27481 atggctgtta aatcggccat tgcaatcgat acccagcaga accaatacgt ccagccagtg
    27541 atgaaagccg cttttttgcc aagatattct gcaacaaaat ccagaaaaga atggttattg
    27601 gtgtttgata acagaagttc gcctaacgca cgcataatga aaaaacagat gcatccagta
    27661 ataagatagg cgagcaatat cgaagggcca gctagctgga tagactttcc ggaacctaaa
    27721 aacaaccccg ttccaatcgc tccgccaata gaaatcagtt ggacatgtct atttttcagt
    27781 ccacgctgca gttcttgctc ttcattcatt tttaaatcat cctaactaaa aaagtttaat
    27841 tacttccata ttaaaagagt tttctgaaaa acacaactaa gattccaaaa ttttcagaaa
    27901 tttacctttt atgagtaatt cataaaaaag aactgcggta aacaccgcag ctctttgtcc
    27961 caatcagcta ttgatcctaa gcatttaaaa tacgtgaaag gaattctttt gtacgttctt
    28021 ctttcggatg gacaaagata tcttctggtg taccttcttc tgcaatcaca cctttatcca
    28081 taaaaatgac acggtcagat acatcttttg caaattccat ctcatgcgtc acgataagca
    28141 ttgtcaagcc agtatgtgct aagtctttga tcgtattaag cacttcgcca accatttcag
    28201 ggtctaacgc cgaagtaggt tcgtcgaata gcaatacatc tggattcatt gataatgccc
    28261 gagcaattgc gacacgctgt ttctgtccac cagataattg tgctggtttg gcttcgatat
    28321 aacgatccat accaactttt tccagatttt cgattgctac ttttttggca gtttctttat
    28381 ctcgtttcag gacagttgtt tgaccagaca tacagttttc caagacattc atattgttga
    28441 acaggttgaa ggattggaaa accataccta gattcgttcg atatttaggt aaattatatt
    28501 taggagctaa tacattttct ccattataaa tgatctcgcc gcctgttggt ttttccagta
    28561 aattgataca gcgcaagaaa gtagatttac cagaaccgga agaaccgatg atcgtcacaa
    28621 cttcaccttt attgactgtt acgctgatat ctttcaatac ttcattttca ccgaagcttt
    28681 ttcttagatg tttgatttca ataatttttt cattcatgat tcttcctcct tattcctgca
    28741 aatttgaatt gtcgatttct tcaagcttgg tataagctga aggaccatcg attttctttt
    28801 ctaccgcccg caggattctg gtgatcgtaa aggtcatgac taagtagatg atcccgacga
    28861 tcgtaaatgt ttggaagaat tggaaattcg caccggaagc agagtttcct tggaagaaca
    28921 aatcagctac gccgattaca cttaaaactg ctgtatcttt gatattgata acaaactcat
    28981 ttcctgttgc tggcaagata ttccgtaaaa cttgtggaat aacaactttt ctcatcgttt
    29041 ggccatgggt cataccaatg gcttgcgcag cttcgaactg cccttcatct actgcaaaaa
    29101 tccccccacg aacgatctct gtcatgtaag ctccagtatt gagagagaca ataaacaaag
    29161 cggcaatcgt tcggttcaat gaaatgccaa aagcgagtgc cagaccgtag aagatgacca
    29221 ttgcttgtac catcatcggt gttccgcgga atacttcgac atagatagaa agaagtgcat
    29281 ttccgatttt ttggaagaag cgagtcagtg catttcctga ttcaggtacc gaacggaaga
    29341 caccaatcag taatccaagt gtagtaccga caactgtccc gatcaatgcg atgaacaagg
    29401 taagtccagc acctcgtaag aacatattgc cgtattgttt ccaaatcgta gtgaaatcag
    29461 aaatgatgcc aggtttttct tcactatcag ttgcagcagg ctgatctttg actgcttggt
    29521 ccataatttt ggttcgttca tcgttagaga tgccagaaag aatctggttt actttttgga
    29581 tatccgggtc gcctttacgc ataccaacag aaatctgaac atcttccggg tttgtctgga
    29641 agccatctgc accgctgaat tcaagcattt ccaagtcttt gttcacacta gttgccgtta
    29701 caccttccgg acgttcgctg acataaccat cgatcattcc agaagctaaa gctgttctca
    29761 ttgctgagaa attatccatt gcttgttgtt tctgcacatc tggtatctga tcgatcacag
    29821 tatagtggaa tgtatttaac tgagcagtaa tctttgctcc agaaagatct gctaaacttt
    29881 ttgcttttgc atatttgcta tctttttgaa ctactactac taattgtgat tcgtaataag
    29941 gatcggtaaa atcaatttct tttcgtcgtt cagcagtagg gctcattccg gcgatgatcg
    30001 cgtcgatctt gcctgattga agggcaggag ccagaccatc ccatttcgtt tggacgatga
    30061 ctaatttttt ccctaaacca tctgctactt ttttagcgat ttgaacatca taaccgccgg
    30121 cgtaactatt ttggccttga attggcaagg cattattctc atctgtttgt tgagaccagt
    30181 tgaagggtgc atacccagcc tccataccta cgcgaaactc atcgctcggt gttttttcat
    30241 ctgcatttgc cagagatccc acactgaata gtgagaagac aaaagcaaat agtacggcaa
    30301 ctgaaaccat attttttttc atttttttct ccctttctga ttatatgata cacgagcggt
    30361 ctagatgaaa tgcggtaaag ctttatctga aaaaataaaa agcgaccgat ttatagatat
    30421 aaaaaggccg ctaaaattcc ataaggtgat aaacctctca caaaacatag cgcaacttag
    30481 catcactgct aagacagtcc gcaagctctt aacttgcgtc ccaacataca ctccaagcaa
    30541 aaaagtgcac atttcggcga tcctccctat tcctgcttct ccatgtttgc ttcactccac
    30601 agggttaatg attctcgcga cctctacctc attgattgag gcatctcgta ttcagttatt
    30661 tatacaaaaa aagtttacga tactcgagag gctttgtcaa tctataactg ctgttttttg
    30721 aaaaaatact gataaatata gaaatcttta cttttaccga aaaaacaatc aaaatgtgaa
    30781 tcatctatcc attgcaaacg atagtataat tatacacaaa aatgtatgta atacaataaa
    30841 aaaagaacta gaacagaaaa tttcctgtgt ccagttccat ctcgatcatt taagctgccg
    30901 ctttctttgg ctcacgtcct aggatcgctt gcaaaagaac aacgacgcca taaatggcta
    30961 atattagata aatcaaactt gagtattggg tgattcccca gccccatgtt cgatatacgg
    31021 ctaatgtaat ggctaaaaca attgcggcaa tcacgttgaa taaagcaaaa gcccaaagat
    31081 ttccattttc tttgcgtaca agataaaaaa cagagaaata aacgcttaac gctaaccagg
    31141 cgatacaagc cagaataacg ccccaataaa taaagaatgg taacatatcg attcacctca
    31201 cttttataca gcagtattgt aacacgtttt cattcttttg tcagtgaaca ttgtgataaa
    31261 ttgttcaaat gcccataaca cttttttatt cccatttttc tgacttttca acaataaatt
    31321 tgttcgagta tgggctataa tggaaagtga ggtgacaaaa catgaaacaa ttaacagtag
    31381 acgaagcagc aaaagagata ttagcttatc agcaaattac atttttgacc ggtgcagggg
    31441 tatccacacc ttctggagtg ccagactatc gatctttgtc aggaatctac cacgggatgg
    31501 agcagccgga atacttgctg agtcatcaag caatggtgaa tgaaccggat aaattctatc
    31561 aatttgtcaa aaagatttat catcctgaag caaaacccaa tatcattcat ttggaaatgg
    31621 cacatctagc gaaagaaaaa aatgtttggg ttgtttccca aaacatcgac ggtttgcacg
    31681 caaaggctaa aagcccgcaa ctcgtcaatt ttcatggaag tttgtatcaa tgtcattgtc
    31741 gaaaatgtca acaaacggta gactggaaag aatatcttca cagcgacaaa catcaagcat
    31801 gcggaggaca aatacgccca gacatcgtgt tatatggtga agggtttcaa gatggagtca
    31861 tggagcaggc ggcttatgcc gtcagccaag cagagctgat cgtcattgtt ggaacgagtt
    31921 ttcaagttca tccgttttgt gatctggtac agtttcgcca gccgtcagcc aaaatcttag
    31981 taatcaatca aacgcctgtt tatttagcgg agccttatac gtttattcaa gaagacggag
    32041 cggttgtttt caaaaaaata caagaattag gagcagatta tgactgaatt caaaaccgaa
    32101 aaagaaaaaa tgattgcagg cgagctttat tttgccaatg atcctgagct ggttgccgac
    32161 cgtatgtttg caagaagtca aagccaaatc atcaaccaag cagaatcagc ggaactgcga
    32221 tcccaattgt tgaaagagac ttttggccgt acagggaaaa aaatctatat ggagccagtt
    32281 atcaattttg attacggata taatatattt gttggggaga atttttatgc aaatttcaat
    32341 tgtacatttt tagatgtcag cacgattgaa atcggtgata actgcatgtt cgctccgaat
    32401 gtccagcttt acacggcgac gcatccacta catccagtga aacgtaacag cggtctggag
    32461 tacgcaaaac ccattaaaat cggtaataat gtttggttag gcggaggagt tatcgtcacg
    32521 ccgggtgtca ctctagggga caacgtagta gtcggcgcag gttcggttgt gaccaaatct
    32581 tttcccgaca atgtagtgat tgcaggaaat cctgctcgta tcatcaagac agtagaggaa
    32641 gaactaacag aagagtctct tgaaacatta cgtcataaga tcgatatgat cgatcgagag
    32701 ttgattgctt tattagaaaa aagaatggat acggtaacta aaataggaca agtgaaaaaa
    32761 gaagaaaacc aagcagttta tgatgcaaaa agagagcagc aagtattgga taaagttgtt
    32821 agtctgctga aaaataaggg atataaggaa acaataacag atacgtatgt tgatttgatg
    32881 aaacactcgc gcgaatacca aaacaaaatg aaggaggaat aagtgtgatc gaaggagaga
    32941 cacggcgaaa acaaatcatg caaaccttgg caaatacaga aaaaccagtg agtgctagca
    33001 agtttgcgaa aacttttgga gtcagccgtc agatcattgt aggcgatatt gctttactac
    33061 gagctgcagg taaaagtatc gtggcaacag caagaggtta tatcctcgaa acagaagaaa
    33121 ccaatactgg gcacatcagt aaaatcgctg tccagcatgg aaaagatcaa acagaagaag
    33181 aactgagact aatcgttgaa aatggtggtg aaatcattga tgtaattgtg gaacatcccc
    33241 tttacggtga gttagcagga actttgcaca tcaaaacgcc acaagatatc gattccttta
    33301 tgagacgtta tcaaaaaagt aatgctactt tgttatccga attaacaggc ggcatccatc
    33361 tgcatacgat ccgctaccca gaaaagcata tgctgaaaaa catcaaacaa aaactagctg
    33421 aagcaggaat cctctacgaa ggataacatc gtagctgcta ctttttatcc tagaaactat
    33481 tctatcatct ctgagataaa cacccatatg gaagaaataa gaaaaaataa gaaaagatgc
    33541 gtatccaata cacagaattt tttggaattg cgatttctag atgtctatct aaatttcgat
    33601 tagtggagaa acataaaaaa gggaagtgca gcttttgagc actccccttt tttataaagc
    33661 tttcttgtag catagtatcg aaggaacctg ccaattctct actcgatcac caatacgcct
    33721 tcttttagtg caaatggatt ttgatcgttg atgtggtcat agaacatcac accgttgata
    33781 tggtcgattt catgttgaac gacgatcgat tcataattct tcaaacgaat cttgtgtttt
    33841 tcaccattca tatcataata agacacagtg attttggcat ggcgaacaac atatcctggt
    33901 acttctcgat caacagataa acagccttca ccttccccta aacaagcatc ttgaacagaa
    33961 tggctcaaaa ttttaggatt atacataaca gtacttagag aaggtccatc tgcctctggg
    34021 tcaggacttg gtacgtgaac agcaatgatt cttttagaaa tatctaattg aggagcagct
    34081 aagccaacac cgcctcgtaa gtgtagttct tcagctttga ttggatcttg gctgtttttt
    34141 aagaactcaa gcatctcttt tcctaaagag atatcttctt cgcttaatgg aagagaaact
    34201 tcttttgcaa cttcgcgtaa ggtcggattt ccttcacgaa tgatatcatc cattgtaatc
    34261 atggaatatg tacccctttc ttaaaagact gtgtttcaag tctattttcg cattatagtt
    34321 taccacaaaa atggaaaagt agtagtaaga ttgtttgaaa actacctttt cttaatgatt
    34381 aactacttga ttttcaaccc ggtttcaggt aaagtatagc tgtgtgctct aggcacatga
    34441 cctgccactt ggttaagcga atcaccaacg ttttactcag tcgcagggga ataatcgaaa
    34501 gaggtgaaag aaatggcaat ttcaaaagaa cgcaagaatg aaatcatcaa cgaatacgct
    34561 cgccatgaag gggatactgg ttctccagaa gtacaaatcg ctgtattaac tgaagaaatc
    34621 aaccacttga atgaacatgc acgcgtgcac aaaaaagacc atcattcata ccgcggccta
    34681 atgaaaaaag ttggtcaccg ccgtaacttg ttagcttact tacgtaaaac tgacgttcaa
    34741 cgttaccgcg aattgatcaa acgtttaggc ttgcgtcgtt aattttagtc aaaaagagtg
    34801 ggattcacat gaatctcact ttttttactc gtatatcaat taagtaaaat tccgtgatca
    34861 agctagatgg actcctacta accaaatgaa agctgctcaa aagacggctt tcggcagttt
    34921 tcatttgttt agtagcgtct tgctttcacg agaaggaaaa ggagaagtta tgtcagaaaa
    34981 acaagtattc aaaacgacat ggggcggccg tcccttacaa atcgaagtcg gtcagctagc
    35041 aaaacaagca aacggagccg ttttagtccg ttatggagat acagtagtct taagtgcagc
    35101 tgtggcctct aaagaagcaa aagatacaga ctttttccca ttaacgatca attatgaaga
    35161 aaaaatgtat gcagcaggga aaatccctgg aggtttcatc aaacgagaag gccgtccaag
    35221 tacggaagca acattgactg cccgtttgat cgaccgtcca attcgcccga tgtttgcaga
    35281 aggcttccgt aatgaagtcc aagtaacgaa catcgtgatg agtgtagaaa cagactgctc
    35341 gccagctatg gcagcaatgc taggttcttc tttagcacta tcaatctcag atatcccatt
    35401 tgacggccca atcgcaggcg tagaagttgg tcgtgtaaat ggcgagtacg tcttgaatcc
    35461 aacagtagaa caagcagaac aaacagatat cgaattgact gttgccggaa cgaaacaagc
    35521 catcaatatg gttgaaagcg gcgcgaaaga agtttcagaa gaagacatgc ttggtgcatt
    35581 gctgtttggt tttgacgcta tcaaagagtt agttgctttc caagaagaaa tcgttcaagc
    35641 agtagggaaa gaaaaaatgg aagtgacttt actgcaagtt gatgaagtat tgaaaaaaga
    35701 aatctttgat gcttcttacg ctacaatgaa agcagcagta atgactgaag aaaaacttgc
    35761 tcgcgaagat aatatcgagc aggtgaagat cgatatccgc gaagcttatg ctgagaaatt
    35821 tgcaggtcat gaagatgaag atcacttgtt gaaagaagtt aaacaaatca cagaagatct
    35881 tgaaaaagat gtcgtacgtg aattgatcac catcgataag atccgtcctg acgggcgtaa
    35941 attggatgaa atccgtccat tgtcatcaga ggtcagctta ttgccacgtg tacatggatc
    36001 aggtttgttc acacgaggac aaacacaagc tttatcagct tgtacactcg cacctctagg
    36061 agagcaccaa atcattgatg gtttaggggt ggaagtcagc aaacgattca tccaccacta
    36121 caatttccct caattttctg ttggttctac aggacgtgca ggctctccag ggcgtcggga
    36181 aatcggacat ggcgcattag gtgaacgtgc gttagcacaa gtaatccctt cagaagaaga
    36241 atttccttat acgatccgtt tagtagcaga agtattggaa tcaaatggtt cttcttccca
    36301 agcaagtatc tgtgctggca cattagcatt gatggatgca ggtgtaccaa tcaaagcgcc
    36361 agtcgccggt attgcgatgg gacttgtttc tgatggtgaa aactatacga tcctaactga
    36421 tatccaaggg ctggaagatc atcttggcga tatggacttt aaagttgccg gtacaaaaga
    36481 tggtatcact gctttacaga tggatatcaa gattcaagga atcacagaac aaatcctaac
    36541 tgaagcatta actcaagcaa aacaagcacg tatggaaatc ttagaagaat tgacttcaac
    36601 gattgctgct ccacgtgaag aattaagcca atatgcacca aaaatcgaaa tgatccagat
    36661 cgaacctgct aaaatcaaag atgttatcgg taaaggcggc gatacgatca acggaatcat
    36721 cgatgaaact ggtgtgaaaa tcgatatcga tcaagatgga aaagtaagta ttgcttcagc
    36781 tgatgcagaa atgatcaaaa aagctatcaa gatcatcgaa gatttgacca aagaagtcaa
    36841 agttggtgaa gtttatctag ggaaagttgt ccgtatcgaa aaattcggtg cattcgtcaa
    36901 cttgatcaaa ggaaaagatg gacttgtcca tatttcccaa ttagcaaacg atcgtgtcaa
    36961 taaagtcgaa gatgttgtta aactaggtga tgagatcctt gtgaaagtaa ctgaaatcga
    37021 taaacaagga cgcgtaaatc tgtcaagaaa agcaatgttg aacgaagatg gttcaacgaa
    37081 aaatgaagaa aaataattta attcatagga ataatgtact tgtgatcgtg aaggtgacag
    37141 ggggacaata attattcgaa aaaaggggac tgtgacaaaa gccgtgtcac agtccttttt
    37201 tttcgaataa acggtgttca aaagcttgca tctgcggtaa taagtccagc caagttaaaa
    37261 acattaggag ctgtttttat ttggctgttc ttattacttg atgcagaaca gcttttttac
    37321 aacctctcca ataaacgatg ttcaatcagc tgtccctcga tattagctca aaaaacggca
    37381 aaatatgagg aactattttt ccgtttgttt ctctaatact caggtcaaac cgctgatttc
    37441 acaacctctg attcacggtg ttccagaagc agcttctacg gaaataagct gaacactttt
    37501 gttacaactt ctttttttct ggagtttctt ggcgcatatt tttggagtcc ttctgaataa
    37561 agcgtaaaat aaaagtgaga aatatggata aaggagggaa aggagatgct gaatttttta
    37621 tggtcgttat ttatcggcgg tatattaggt ttgatagctg gcgcaatttt aggaaaggaa
    37681 gtcccgggag gcgtatctgg aaatatcatt atcggttttt taggtagttg gctcggcgaa
    37741 tttttactag gaccactggg accggaactc ggcggttttt atttgatacc agcattattg
    37801 ggtgcaattt tttgccttgc tctttattca tttgtggcaa gacgtatcag agaacgtcca
    37861 aagaaagaat aattttattc aaataagttg acacttcagt gtgttacttg gataattaga
    37921 agcaatttag gaaagcgggt gtcatggata agaatatcat ctatcattca agcttaccta
    37981 aaggatatcc atataacaag aaatgaggga aaaacgtggc aacaacgcac aataccaaaa
    38041 agatatcaat gactggcctg cttgtagcga tgggagtcgt ctacggagac atcgggacaa
    38101 gtccgcttta cgttatgaaa tcgattgttg aagataatgg cggattgtca ggaatcaatc
    38161 ctgattttgt tatcggatca gtctcattga ttttttggac attgactcta ctgacaacaa
    38221 tcaaatatgt cgtgattgca ttgaatgcag ataaccatgg tgaaggtgga attttctcac
    38281 tctacacact tgtccgaaaa ggtgggaaat atttgatcat tcctgcaatg atcggaggag
    38341 ctgccttact agcagatggg gtattgaccc ctgctgtgac ggtaacgact gcgattgaag
    38401 gcttgcgagg aattccgcag ttttttgacc ggttcggtaa taaccagaac gtgatcgtca
    38461 ttatcacttt agttattatt tttgttttat tcatggtcca acgctttggt acagatattg
    38521 tcggaaaagc attcggacca atcatgttcg cttggtttac ttttttaggc gtaatgggat
    38581 taatgaactt cagtcaagac tggacagtta tccgtgcact gaatccttat tatgctatcc
    38641 agctgctttt tagtgcagat aacaaattag gcttgttcat tcttggaaat attttcctgg
    38701 ctacgacagg tgcagaggcg ctttattccg atcttggaca tgtcggcaaa cgaaatattc
    38761 gtctgagctg gccttacgtc aaaatttgtt tggttatcaa ttattttggg caggctgcgt
    38821 ggttgttgaa tgtttatcaa aaccctgaag cacagcaaat caagaacctg aatccattct
    38881 tccaaatgat gcctcaaagt tggatcgtga tcggcgttgc ttttgcaaca gtcgctgcag
    38941 tcattgcttc gcaagcattg attacaggtt cgtttacgct agtttcagaa gcaatcaagt
    39001 tgaaactgct gcctcggtta aagatcatgt atcctgggaa cagtatcggg caaatgtata
    39061 tcccagcagt caatctgatt ttatggatcg cctgttctct agtggttgtc acattccgaa
    39121 cgtctcatca tatggaagct gcttacggtc tatcgatcac tgtgacgatg ttgatgacaa
    39181 cagcactgct ttacttttat cttatccaaa atgggcattc tcgttggctt gcatatttag
    39241 ttaccttttt cttcggtgcg atagaagtcg ttttctttat ctcaagtgtt gtcaaattct
    39301 tccgaggcgg ttttgtagcc gttctgattg gtttgatcat cttagtcgtg atgtttattt
    39361 gggaacaagg gaacattatt cgcgaatcgt caaccgagga tgtgtctttg agagattata
    39421 tcccgcagct tagagaatta cgtgatgata cttctttacc aatctatcag acaaatgtgg
    39481 tcttcttggt tcctgatatg gctgaagata aagttggccg acagttcatc tattcgattt
    39541 tagataaacg gcctaaaaga gcacgagtct attggtttgt tcatgtggaa gtgacggatg
    39601 agccatatac gaaagaatat caagtagata tgatggacac tgattttatt gtccaagtga
    39661 atctgttctt aggtttcagg gtccaacagg aaatcaacgt atatgttcgc caagtcatcc
    39721 atgatttgat gaaacaaggc cgtttgccaa aacagccaca aagatactct ctgacaccag
    39781 ggcgagaagt tggcgatttc caatttgtcc tgattgatga agtagtttcc aatgtgacaa
    39841 ctctaggaaa atggcaacgt caaatcatgc aggcaaaact ggcaatcaaa aaaatcgcta
    39901 cctcgccaga aacatggttt gggctagagt acagcgaagt aaaacatgaa actgttccgc
    39961 ttatcattgg aaatactcgg aaaacatggt tgaaagagcg gttgtgaaag tagcgctttg
    40021 acctgaacta atgtcgaatg tcagctactt gaacaccgtt tatcagaggt catgaaaaag
    40081 ctgtactgca acaagcagta agaacgagca gataaaaaca gcttctcatg tttttcatct
    40141 gtttgggctt aatgccgtag atgcaagctt ttgaataccg tttttatcag aggtcatgaa
    40201 aaagctgttt agcaacaagt aacgcaatga ttttgaagtg ataaggagat gtggctgaaa
    40261 aatgccgcat ctcctttttt attcctaaat atgaatacgc attattttca atctttttgt
    40321 agaaaaaggg tacaatagaa aaagagaaag gcgggagaat atggttatca atttcattat
    40381 tttatttttg atgattgcaa ttacttcatt ttttgtagca agtgaatttg ctttagtgaa
    40441 aatcagacga tcaagattgg aacaactaga aaaggaaaat gtaaagaacg ccaagctagc
    40501 tcttcatgtt acacaccatt tagataacta tttatcagct agtcaattag gaattacgct
    40561 aaccggtttg atcatcgggt gggtcggtga aggatctgtc gcggctttac tagaaccagt
    40621 gatcggtaaa ctacctttta gttcggctat tagcagtaca atctctgtgg cgctgggatt
    40681 tattttagtt acgtatatcg atgtggttat tggcgaactg cttcctaaga gctatagtat
    40741 cgtcaatacg gagaaggtcg tcttgttcgt agttaaacca cttcattatt tttataaagt
    40801 aatgtttcct tttatttggg tattgaatca ttcagctgca gggctaggga agctattagg
    40861 tgtgcgtctc gtatctgaag gagaagagac attgtctcaa gaagagctga cattagtcgc
    40921 attgaattct tatgaaaaag gagagttgac caaagaagaa tattattatt tggaaaacgt
    40981 gtttgaattt gatgatacct tggcaaaaga tatacaagta gaccgtacgt ccatgcaagt
    41041 attcgaagct tcagaaaccg tcaaagaagc gataaaagct tcgattgaac aaggacatac
    41101 tcgctatccg gttatcttag aatcaaaaga caatgtttta ggttatgtca ccttgccaga
    41161 tttgatcaag caatcttata aagatgacca gctgacagta gaacagttga ttgaagagcc
    41221 gatcgtaaca actgaaacga tccctataaa aaaattattg acgatcatgc gaaaaaaggg
    41281 taagcatatt gctatcttaa aagatgaata cggagggaca agcggcttag tgacgattga
    41341 agatatttta gaagaaatcg tcggggaaat ccgagatgag acagatttgg acgaagcatt
    41401 gatagcggaa cagtcagatg gctcttatat catctccggc aaactaacat tagatgattt
    41461 ccagcgttat tttcatgtag agattccaga gtttgaagaa acgaatttta ctacacttgc
    41521 gggctttgct tctagccgat ataaagaaat aaaagcagga acaatcattg agattgcatc
    41581 attccgtttc acggtattag aataccagca tgcacatatc gattatttca aagtagagtc
    41641 cacggaaaga aaaacagaat aaaagaaaaa aggctgtgac attttttgtc acagcctttt
    41701 tgttatctaa aaaacctgtt ttcggtaaac gaagaagata ttttcaataa aagttttcat
    41761 ttttagccgc aacgatttct tttaatcaag gctatcttgc ttctgcatgt tctaatgtga
    41821 aatgttcctc ttcacccatg tgtttcaatt gtttcatcga acgcatataa gagtaaagca
    41881 acacggcaac agaaccgatc caagctaaag gagaagctgc tgctgctcct gggtatcccc
    41941 acattcctgt aagaacgata gctgcaaaag aacgcataag caattccatc ataccggcga
    42001 tcgtagggat cttgctttgt ccaagccctt gaagtgtgta gcgtaaaatg aacaggactg
    42061 ctaagatcca gtaaagagac ccattgatat tgaagtaggt ttgtgctaat tcaaatacac
    42121 gtgtttctga tgggttaaca aacaattgaa caaagaaatg accgaagaaa atgacgatag
    42181 cacctgctaa gagactaaat ccgacactca tcagtaaagt ttgacgtacc ccttgcaaga
    42241 ttcggccata ttctttcgca ccatagtttt gtgcagtgaa agttgccatt gcaataccaa
    42301 atgacatcat cggctgattt gcaaattggt cgatacgacc agcagcggct tgagcagcta
    42361 cgacatctgt ccctagacta tttagtgctg cttgtaacac gatagcacca acagcgatga
    42421 tcgaagattg aaacgccatt ggcaaagcag catttaggtg tacagcaaac tcttcttttt
    42481 caaatttgaa atcttttttg cgtaactgta acaaaggcat ttttttcttg atatacacta
    42541 gacacagtac gctggaagaa acttgtgcga ttactgtagc aatagctgca ccttccacac
    42601 ccatgccaaa atagataata aagatcaaat caagtacgac gttgatgatg accgcaatga
    42661 tcagaaagaa tagtggtgtg cggctgtcac caagtgcgcg aatgacatta gaaagcaaat
    42721 tgaaactgac tgatgcaaag atccctaata aaataattga aataaatgtt tgtgcttgat
    42781 caagtatttc aggaggagtt tgcatcaatt gaagcatcgg acgaataaac aggagactta
    42841 ggacagttaa aatcacagtg acgacaagac taatgacaac actagcagca aaacttttct
    42901 tcagaccccg ataatctttt gcaccgtaac gttgtgcggt aataatggct aaaccagctg
    42961 ttaaaccttg ggcaaagcca atgatcaaaa atgtcaggct tccagttgca ccaaccgctg
    43021 ccagcgcatt tttacctagc gtctgaccca cgatgatcat atcgatcata ttgtagaact
    43081 gctgaaaaac atttccgatc agtaaaggaa cagtgaacat caaaatcagt ttagcggggc
    43141 ttccttttgt taaatcgcgc atcttttaaa ctccttatat ctttaaaatt tagttagtag
    43201 tatatagcgt ttatcctgag aataccaatt agaaatcaat gataatttat gaaactccat
    43261 aaaaagaagc caagaattga gtcaaagaat caacaacttg gcctggtctg taaaataagg
    43321 gacatccacg tttatctatc accttaatat tgtctaatct cacttggtaa aaggatcgtt
    43381 tctctcgtac cgtcatcata ggcgtatact tgagcaggaa attggttcga ataggcaaac
    43441 ggcaaatcaa tatccatttc cacattgctt cctgcttgag aaaaatgcat cgtcactttt
    43501 ccacgatttt cgatcaaatc aaaggttaat atattttcta atggaatgac tcccttaaga
    43561 tctaggtcaa taataaacca tatactgtca atcaattcat ctggcaaact tgatacgaca
    43621 ccaactgaag cgtaacggtt gcggctgcta tcaaaacttt caaacatctg tttaacctcc
    43681 ttcgaatcaa cgaatgtttt atttttataa ttaatcctag gataaactag aaatacttac
    43741 ttcaacgagg gttcattcta ataaagaatc aggaaaaatg caacaataat tgaatacttt
    43801 ttttcaaagt ataaattttt ataaaggtac gataaaagta tgcgaggaaa agcttggtaa
    43861 attaggattt ttcgtttgta aagccattta caaaagtgga agccccatat gaaaataaac
    43921 gattagaaat aaaaaaggcg tgttaaaagc tgctgacccg ccgcttctaa caccacctga
    43981 ttgtttcagt attattcttc tgttgaaccg atcacttctg gttcgccacc ttcacctgtg
    44041 ctttcttctt cttcactttg agcgactaca gcagcgattt gctcttcact gtcggtcacg
    44101 aatttgtaat cgtcgttttt tggaagatcg gctactgtaa tagaatcacc taattccaag
    44161 tttgtcacgt caacttcaat tcgttcaggc aatttgtctg gtgtagcaga tacgactact
    44221 gtatacaagt tttgagctaa cactccacca gatttagcac ccactgattc gccaacaaga
    44281 acgacttctg tttcgacttc tgtttcttcc gacatgttta cagatagaaa ttctgcatgt
    44341 tcgaaatgat tggtaaaagt gttcgtttgg atcttgtgaa ccaatgtatt gactttttgt
    44401 ccatcgatgt ccatcgtgat gactgtgttc gctccatgtt cacgcaacaa tttgcttagt
    44461 gtcaaaccat cgaatgagat aggggtactt tctactttat acccataaac aacagctgga
    44521 actttccctt cgtgacgaag tttgtttctt aatgaacggg gacgtacttc tcttttactt
    44581 acttctaatg atactgacat aaccaaaatt cctccttaaa atgtgactgt ctgctttggt
    44641 aacggacaga ttttatttaa gtgaattcta aaggctaatc tcgtgcacat catcgaaggc
    44701 agggcgctta aaaaagcgca aaaggagaga tgcgcgcatc tccccttaga aagtcaggat
    44761 tcaacttttc tccaccaggt cattacgctg tgtatgagaa atccaattca ctttactatt
    44821 taaccgtaac gtgaactgtt aattaagtca aggaataact atgatgatgt atttgcaagc
    44881 aagaatattc aaggtataat taattagtag taataagaaa ggaaacagca tgcgtttaga
    44941 taaattaata gaagaaaaat tagaaacgac acgcaaggaa atgaagcggc tgtttgcttt
    45001 gaagcaagtg atgatagatg gcacagtcga atttcgacaa aatcgaaatg tagatagttt
    45061 actgcatcaa atcgaagtag caggtgaaca cttagaaaca aatgagtcct attatcttct
    45121 caataaacct gaaaaagctg ttacagctgt atcagatcca gaaaagacga cagtaatcga
    45181 tctgattgca agtgccgatc gtttccaacc attatatcca gtgggaagac tagatagaga
    45241 tacaacagga ctattgctga tcacttcgaa tggacaattg ggatatgaaa tgctacttcc
    45301 agaaaaaaag gtttcaaaga cttatcaagt agttgtgaac gaaagggtga caccggcaga
    45361 cgtaacagca tttgaaaatg gaatcgtatt cgctgatggg tatcaatgta agccggctga
    45421 tttaaaaata ctcaaagcag acaagcacca aagtacggtt ttattgacga tccaagaagg
    45481 aaaattccat caagtcaaaa agatgttctt agcccaagga aagaaagtga ccgcattgaa
    45541 acggctaagc atgggaccat tggaattaga tgaacagcta ggagtaggcg agtatcgatc
    45601 gttgacatta gcagagatga aaaagttaag tatttatttt aaataggagg tcaaaaagat
    45661 gaaatataaa actttattgt ttgatgtaga tgatacattg ttagattttc aattaactga
    45721 agccaatgct ttaagtgctt tgtttgcaga acaaggaatc acattgacac cagaaatcga
    45781 aaccagctat aaggctttga atcatcattt atggcaggag tttgaaaaag gcagactatc
    45841 acgagaagaa ataataggca gtcgttttgg gttacttttc cagcaatttg ggaaaacagt
    45901 agatagtgat caaatggaaa aacgctaccg ccactacttg aaccaaggtc atgatcttgt
    45961 tccaggaagt ctagaaatcc tgaaaaaggt ccatccgcat gctgatcttt atattgtgac
    46021 gaacggtgtg tcgcacacgc aacatcggcg attaacagat gcaggaatgc ttgattactt
    46081 caaggatatc tttgtctcag aagctgtcgg agcgcaaaag ccgatgaaag aattttttga
    46141 tcatgtcttt tcccacattc ctaattttga taaaaagcaa actgtgatca ttggtgattc
    46201 tttgacttct gatatcaaag gaggaaatat ggcagggatc gatacgattt ggttcaatag
    46261 aaataaatta ccagaaatcg aagaaatcca gcctacttat cgaatcgatt cattagagga
    46321 actttattca ttattggaaa ttgaaaagga ttaattttca gaaaaatata gcattttcgt
    46381 gttttttcgc tataatagat tagaatgaaa aaacggatac gaaagaggtt ttagtcaatg
    46441 acaaaaagca agccaattat cattggtgtg acgggtggat ccggaagcgg aaagacaagt
    46501 gtcagccgtg cgattttcaa caacttcccg gaccattcca tcatgatgct tgaacaagat
    46561 tcttattaca aggatcagag ccatttgagt tttgaagaac gattgaacac aaattacgat
    46621 cacccatttg cctttgataa tgatctgctg attcagcatg taggcgactt actgaactat
    46681 aaagccattg aaaaacccgt gtatgactat gtagcgcata caagaagtca ggcaacgatc
    46741 attcaggaac caaaagaagt gatcattcta gaaggaatcc tgattttaga agacgaacgt
    46801 ctgcgtgact tgatggatat caaagtatac gtagatacag atgatgacat tcgtatcatt
    46861 cgtcgtatca aacgggatat ggaagaacgg ggacgcacat tggactccgt gatcgaacaa
    46921 tatttgacag ttgttaaacc gatgtatcat caatttatcg aaccaacgaa gcgctatgcg
    46981 gatatcattg ttccagaagg cggagaaaac cacgttgcta ttgatttgat tacaacaaaa
    47041 gtagctagtt tcttaaacca taaatagtgc gcagcgaaac agttgaacgg actgcgaccg
    47101 tttgaaaagc aaggattctg ctttttgaac agtcaccagc caactgtttt tttatttata
    47161 acaagaggtt gtgaaaacag catcttgacc taagtattaa aaagaagaaa cggaaaatag
    47221 ctgtttatat tttaccgttt tttgagctaa taccgaaggt cagctgattg agcaccgtga
    47281 agtagaggtt gtaaaaaagc tgtctttgca tcaagcgata agagcatcca aataaaacag
    47341 ctcctcacgt ttttcgttgg gttgggctta ttatcgtaga tgcaagcttt tgaacaccgt
    47401 ttaacgagag gttgtaacag aagtggtcag atgcaataaa taagaagaaa cttacgaaaa
    47461 ttatttctcg aatttgtgtg agatttcgac ttattttttt gaagaagctc ttttgtcgca
    47521 cccc
//
LOCUS       DACCWK010000013        46630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCWK010000013 DACCWK010000000
VERSION     DACCWK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.013"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            34..1326
                     /locus_tag="IVW65_000647"
     CDS             34..1326
                     /locus_tag="IVW65_000647"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7899570.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            2113..2976
                     /locus_tag="IVW65_000648"
     CDS             2113..2976
                     /locus_tag="IVW65_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7899571.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(3105..4727)
                     /locus_tag="IVW65_000649"
     CDS             complement(3105..4727)
                     /locus_tag="IVW65_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7899572.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            4976..5497
                     /locus_tag="IVW65_000650"
     CDS             4976..5497
                     /locus_tag="IVW65_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899573.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(5683..7413)
                     /gene="spxB"
                     /locus_tag="IVW65_000651"
     CDS             complement(5683..7413)
                     /gene="spxB"
                     /locus_tag="IVW65_000651"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7899574.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            7578..8396
                     /locus_tag="IVW65_000652"
     CDS             7578..8396
                     /locus_tag="IVW65_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7899575.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(8499..8696)
                     /locus_tag="IVW65_000653"
     CDS             complement(8499..8696)
                     /locus_tag="IVW65_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899576.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            8865..9443
                     /locus_tag="IVW65_000654"
     CDS             8865..9443
                     /locus_tag="IVW65_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7899577.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(10488..10991)
                     /locus_tag="IVW65_000655"
     CDS             complement(10488..10991)
                     /locus_tag="IVW65_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7899578.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            11350..12375
                     /locus_tag="IVW65_000656"
     CDS             11350..12375
                     /locus_tag="IVW65_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7899579.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            12698..13576
                     /locus_tag="IVW65_000657"
     CDS             12698..13576
                     /locus_tag="IVW65_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899580.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            14052..14168
                     /locus_tag="IVW65_000658"
     CDS             14052..14168
                     /locus_tag="IVW65_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7899581.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            15096..15434
                     /locus_tag="IVW65_000659"
     CDS             15096..15434
                     /locus_tag="IVW65_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899582.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            15528..15758
                     /locus_tag="IVW65_000660"
     CDS             15528..15758
                     /locus_tag="IVW65_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899583.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            16158..16529
                     /locus_tag="IVW65_000661"
     CDS             16158..16529
                     /locus_tag="IVW65_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7899584.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(16803..17495)
                     /locus_tag="IVW65_000662"
     CDS             complement(16803..17495)
                     /locus_tag="IVW65_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7899585.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(17498..18283)
                     /locus_tag="IVW65_000663"
     CDS             complement(17498..18283)
                     /locus_tag="IVW65_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7899586.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            18520..19920
                     /gene="lacG"
                     /locus_tag="IVW65_000664"
     CDS             18520..19920
                     /gene="lacG"
                     /locus_tag="IVW65_000664"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7899587.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            19945..20262
                     /locus_tag="IVW65_000665"
     CDS             19945..20262
                     /locus_tag="IVW65_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7899588.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            20275..21948
                     /locus_tag="IVW65_000666"
     CDS             20275..21948
                     /locus_tag="IVW65_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7899589.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            21962..23593
                     /locus_tag="IVW65_000667"
     CDS             21962..23593
                     /locus_tag="IVW65_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7899590.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(24084..25649)
                     /gene="guaA"
                     /locus_tag="IVW65_000668"
     CDS             complement(24084..25649)
                     /gene="guaA"
                     /locus_tag="IVW65_000668"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7899591.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25708..26631)
                     /locus_tag="IVW65_000669"
     CDS             complement(25708..26631)
                     /locus_tag="IVW65_000669"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7899592.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26827..27432
                     /locus_tag="IVW65_000670"
     CDS             26827..27432
                     /locus_tag="IVW65_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7899593.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27740..28756
                     /locus_tag="IVW65_000671"
     CDS             27740..28756
                     /locus_tag="IVW65_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7899594.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28897..30198
                     /locus_tag="IVW65_000672"
     CDS             28897..30198
                     /locus_tag="IVW65_000672"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7899595.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30244..30906
                     /gene="deoC"
                     /locus_tag="IVW65_000673"
     CDS             30244..30906
                     /gene="deoC"
                     /locus_tag="IVW65_000673"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7899596.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30927..31325
                     /locus_tag="IVW65_000674"
     CDS             30927..31325
                     /locus_tag="IVW65_000674"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7899597.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31391..32473
                     /locus_tag="IVW65_000675"
     CDS             31391..32473
                     /locus_tag="IVW65_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7899598.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32685..34247
                     /locus_tag="IVW65_000676"
     CDS             32685..34247
                     /locus_tag="IVW65_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899599.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34240..35373
                     /locus_tag="IVW65_000677"
     CDS             34240..35373
                     /locus_tag="IVW65_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899600.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35373..36329
                     /locus_tag="IVW65_000678"
     CDS             35373..36329
                     /locus_tag="IVW65_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899601.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36428..37594
                     /gene="deoB"
                     /locus_tag="IVW65_000679"
     CDS             36428..37594
                     /gene="deoB"
                     /locus_tag="IVW65_000679"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7899602.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37618..38436
                     /locus_tag="IVW65_000680"
     CDS             37618..38436
                     /locus_tag="IVW65_000680"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7899603.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38453..39160
                     /gene="deoD"
                     /locus_tag="IVW65_000681"
     CDS             38453..39160
                     /gene="deoD"
                     /locus_tag="IVW65_000681"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7899604.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39458..40144)
                     /gene="gpmA"
                     /locus_tag="IVW65_000682"
     CDS             complement(39458..40144)
                     /gene="gpmA"
                     /locus_tag="IVW65_000682"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7899605.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40316..40897)
                     /locus_tag="IVW65_000683"
     CDS             complement(40316..40897)
                     /locus_tag="IVW65_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7899606.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(41030..41713)
                     /gene="rpiA"
                     /locus_tag="IVW65_000684"
     CDS             complement(41030..41713)
                     /gene="rpiA"
                     /locus_tag="IVW65_000684"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7899607.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            42032..42445
                     /gene="rpsL"
                     /locus_tag="IVW65_000685"
     CDS             42032..42445
                     /gene="rpsL"
                     /locus_tag="IVW65_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7899608.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42529..42999
                     /gene="rpsG"
                     /locus_tag="IVW65_000686"
     CDS             42529..42999
                     /gene="rpsG"
                     /locus_tag="IVW65_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7899609.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            43093..45180
                     /gene="fusA"
                     /locus_tag="IVW65_000687"
     CDS             43093..45180
                     /gene="fusA"
                     /locus_tag="IVW65_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7899610.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45352..46539
                     /gene="tuf"
                     /locus_tag="IVW65_000688"
     CDS             45352..46539
                     /gene="tuf"
                     /locus_tag="IVW65_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7899611.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT        14468 a         8330 c         9810 g        14022 t
ORIGIN      
        1 tcaggattaa ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg
       61 caatggggcg atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg
      121 attgcacggt accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact
      181 tataaactgc atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga
      241 aacggcgttg tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa
      301 ggtgtagaaa caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat
      361 atcaaattgg accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt
      421 aaaggaatcg gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac
      481 ttattagata aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt
      541 caatttgtaa aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat
      601 tatgaatacg gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat
      661 gctttggatg caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc
      721 gaccagggaa cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc
      781 ggaagcggtg tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg
      841 tcacgtgtcg gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc
      901 cgcgaagtag gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt
      961 gattctgtag ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac
     1021 tcgatcgatg tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat
     1081 ggcgaactaa tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc
     1141 tatgaagaat tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg
     1201 ccagaaaacg cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca
     1261 acattttctg tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa
     1321 atctaaggat aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt
     1381 tatctatacg ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg
     1441 ataggctgtc tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
     1501 cagtcccttt ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat
     1561 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
     1621 gctttttcac aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca
     1681 caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa
     1741 acggcaggct cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     1801 caaaaaacgg caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca
     1861 aaacgctgat ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa
     1921 gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
     1981 ctgaccactt ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa
     2041 agtgtgtcta gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag
     2101 gggttgaaaa atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta
     2161 ttagcaaaag aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta
     2221 gtagatgacc tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt
     2281 gcgatgccta cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat
     2341 gcggaaaaac agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac
     2401 agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat
     2461 gtttttgata caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga
     2521 ttgaaaaaag aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg
     2581 aaagtccatt catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt
     2641 tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat
     2701 aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca
     2761 atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct
     2821 tacgctgggg atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag
     2881 atgaaagatg tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc
     2941 tgtatcgcga ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa
     3001 agcccagaaa ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc
     3061 tcttagaggt cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg
     3121 ttcaattgtt tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga
     3181 tcgatcacac ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga
     3241 tggtcatgtg acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct
     3301 gtgattgatt ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc
     3361 gaaagcatca tttttgaaag catgcaacgg actttctctc caccagataa aacattcact
     3421 ggtttcaata cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg
     3481 ttatcatctt cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta
     3541 tcgaattctt tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt
     3601 actgttccag tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt
     3661 atatcatttt ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca
     3721 tctaaaattt tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt
     3781 ccaatctcac gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata
     3841 tcgtctaaag taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat
     3901 gcattcgcac taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg
     3961 gcatttgcgt ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa
     4021 tttcctacat aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg
     4081 ttcaaaaagt gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat
     4141 tcttccagcc aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga
     4201 acatcaggtt taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt
     4261 aattcactca tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca
     4321 gctgcttctg gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct
     4381 gctcgaatac catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc
     4441 agcacttcat acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca
     4501 tagtcgtaat ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa
     4561 cctgtggaag gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca
     4621 tttgcaccaa tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat
     4681 agtttgcggt ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac
     4741 ttttatcatt attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt
     4801 caaggcaatc gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa
     4861 agcggagcga aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt
     4921 tatttataag agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga
     4981 tgaaaaaaga accatcgact ggggctcatt gattttggga attttatttg tacttgtctc
     5041 attgttatca tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat
     5101 ttttgccttt attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac
     5161 aggatataaa ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt
     5221 tttcttattc aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt
     5281 tattgctgat tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg
     5341 atattattgg tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt
     5401 gtttaatcca atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt
     5461 atttggtatc acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag
     5521 ccggcttccg attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa
     5581 atatgcacga ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat
     5641 atacacaaat tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata
     5701 taattcattc aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc
     5761 ttcttcagaa tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt
     5821 attgatcttc acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa
     5881 tcgaagttgc gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt
     5941 accgaaatct gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc
     6001 tgcttcgatg aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata
     6061 tttgacttgt gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt
     6121 aaatacttgt ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc
     6181 catagttgcg aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg
     6241 aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat
     6301 acgattgatt tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa
     6361 agatttcaac caatttaccc atttttcttt attttcttga tttgctaggt accagctatc
     6421 agcagggcga gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag
     6481 gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat
     6541 aaattcagca gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa
     6601 gatcagatcg gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa
     6661 accaagaaag ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac
     6721 aggaactgaa aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg
     6781 accaacataa agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc
     6841 tttttcttct ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc
     6901 gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc
     6961 ataggcggct ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt
     7021 acgattgtat acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt
     7081 atagttcatg gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat
     7141 ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac
     7201 agcgccgact ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc
     7261 atgacgaact tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga
     7321 attgaaagat cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt
     7381 taccattgcg attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga
     7441 tacataaatt ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt
     7501 cttttcacaa aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt
     7561 caagagaggg gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac
     7621 ttgctgggac agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct
     7681 ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga
     7741 ttgctgcaag tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag
     7801 atattgcttg gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg
     7861 cagataaaac actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta
     7921 cccatttaaa acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag
     7981 gtttagccgt gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta
     8041 tattagccgc tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag
     8101 ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac
     8161 aagcttctgg aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag
     8221 tggagctgct gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg
     8281 tggaagaatt gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt
     8341 ttggcgtcat gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa
     8401 aaaaaatata tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt
     8461 agtaagttat atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt
     8521 gtatttcctt tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg
     8581 accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct
     8641 gcaattagca ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa
     8701 aaccctcctc ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca
     8761 cctccaactg aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc
     8821 tctggtttat catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc
     8881 ttgaagcatt tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc
     8941 atgaacctaa cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct
     9001 atattgtcag ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag
     9061 ttgtacataa gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt
     9121 cgctttttcc gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa
     9181 tgacttatgg ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac
     9241 ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga
     9301 aatcgtatct atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc
     9361 cagtagccgt gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta
     9421 ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga
     9481 aaaaataaat aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg
     9541 gatcaaaaaa gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg
     9601 gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca
     9661 gcaataaaaa aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg
     9721 tttacagcag tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag
     9781 aaaagtgtat cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata
     9841 aaaataaaaa attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca
     9901 gaaatgaata aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa
     9961 aaaaataaca tatctcattt tacacttttg ttattttata cttaaaaaat aacgacacag
    10021 aaaataccgt gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa
    10081 atgccttcaa aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct
    10141 aaataaggag aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata
    10201 gaaaaataat atacctgagt agggtataaa ttaagacgga attcctttct agattgagat
    10261 attgactagg aagaatgtta agattttaag cctttaagct aaaaaaaaac cctttttgtt
    10321 aaataagggg aatatagcct gtcagtttca aactgcaagt cgtcctgctg caataatata
    10381 gtagattaga gatgaaaata gattttttta aaccaatgac tgaataaaaa gaccgtcata
    10441 gatacacatt ggaatgtgtc tattacggtc tttttttacg aacgatttta ttgaagacgg
    10501 acagttcggg tcaaaaataa aaaagctacg atcagaaata agaataaaat gtatcccaaa
    10561 ataatggcta gacggtctaa cttatttgtc gatagatgtt tgtttagaag ttgataggcc
    10621 cataaaccta tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc cgctacgcca
    10681 ataccgaata ggaattgaat caattctgcc aagaagctta caaggaaaat aatccctaat
    10741 tttttagaaa aagaaagtct cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag
    10801 acaatcaagt tataagtggt ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa
    10861 aaagggacga gattgacatt gctgtaagaa taagtgagaa attgtggaag attaaatgat
    10921 agcttgaata aaatcaacca taatagaaag aataaataaa tccctaataa aaattttgat
    10981 acataccgca tagacatctc ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat
    11041 cataaaaaag tatgaagttt aaggaccaac gtagttatcg tacgaaaatt agaagctttt
    11101 ttttgttggg aatccggttt ctttaacaaa gctaagatag cgagttgctt ggaaaagttg
    11161 ctatcttttt tctgtcaaaa cgactcggaa tgttcgtttt ttgttctaat ttctccaagt
    11221 tgtttacttt ttgatactaa gtttatgaaa aatcaagaaa tagttgttat actaggtaat
    11281 gctgacaaac gggattggca tttcatcttg gttcagcttt ttttccaatt actaggaagg
    11341 agggatagat tgttcaagac ttattttcgt ttcaataaaa tacaagataa tccttttcgt
    11401 atcatgaatc tgatgctact tatctttatt gtattatggt taggttacat aaatcatgat
    11461 tttttgattt caagtttttc atcattaggg atctttactt ttttctatta tcaaaacata
    11521 ccgatgaaat atttgctgaa acgtatgttt ctgatcggtt gcggactgtt ggttgctttc
    11581 gttttgggga tcttatcgac ttatgtttta tggcttgaac catttgccgt agctttggtt
    11641 gctttttcca gcagattcgt gttgcgatta ttccatattt ctaaaccagg tggcttgttt
    11701 tttgcgatgc tttctgccat gggaacgagc atgcagctgc caatcgctca attaccgatc
    11761 gtctcacttt atttttttat gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa
    11821 ttactggatt cacggcctga gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac
    11881 gaagagcctt tagtgatcat tgattctgtt ttctacagcg ctgccttatt tctttccgtt
    11941 tatgtcagtc acggcttgaa tctgcataat ccttattggt tgaccttgtc atgtgcatcg
    12001 attttgctag cagaaaactt ggatgcgatg aagcatcgac aagtccaata tctgattgga
    12061 tcgatgggtg ggttatgtgt gtcggcattc ttgtcatttg tgccgtttac gcagttgcag
    12121 accatttttc tgatcacttt tctatatgga atcgcacaat ttctagttgc tagaaattat
    12181 gcagttgcta atatttttct taatccgatg gcgttgatgc tatctacact tattcgtgga
    12241 gcatacttga tttctttgat cgaatatcgt tttttaggga tcgtcatagg aagtttcatc
    12301 ggtttaggtg ttgcgtgggt aatgacggtt ggtttgcaac attatttaac ggttgttcgt
    12361 caaaaattag aatagaagga caaatatatg tgccaaaaga ggttatgaca agatttttgt
    12421 cacaacctct aattcaaggt gttcaaaagc cagattctca gcattaagtc aaattcacac
    12481 aaaaatgtgg aaaggaactt tcaaaaaaat tttatattta ttctatcaaa aaattttgtt
    12541 tatgatggta actgaataaa ctttcctgag atggtcaatg ctttccttga aagtcttaca
    12601 gatattcttt cttcatagaa agtttaagtt ttatagttag tgtacaataa aagtatgttt
    12661 ataccatatg gtgtccggat aatcagtcat ttaaacaatg aggttcaaag atatgaaaag
    12721 gggaaatttg atgaagaaaa taagcaatga aatccaaatt ctggcagaaa ctttaactct
    12781 tcccaaagtc gttatagagc aaatgattgt acaagcaaaa aacatccttg atgaggtgtt
    12841 tgagcaatat agtcatgaac taatggatcc taataaagca aaggcgactg caaagaaaat
    12901 agacaaagtc tatgaagagg aaccttttgc agcattagct atttatctat atgcagcttc
    12961 tcaaagttgg aaaaaaattt atcaaccatt gaagattcca agaactatct atttggcaac
    13021 aatgaatgct tttactagat ttattcatga acatttccaa gtaacaggaa gctatcgctt
    13081 tgaccgtggt ttttggattt ggcattacac tagtggcttg atctttcgga ttggtgaatt
    13141 ggagtacgaa atgacatatt ttccgaaagg gtacacggat ctaagattag aaggaaagca
    13201 gtgcctttct cttcatattc cttccgatgc agatttatct cagaataaga taagagatag
    13261 ttacgaaagt gccattcgtt tttttgaact ttattttcct gattaccatt accaagtgat
    13321 gtacacagat acttggttac tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa
    13381 aatctgtctt tttgcagccg attatcgatt attatcagtt gatgaacagg acgatagtgg
    13441 agtcccttgg atctttggac gagtagatgc ccaaatacat gattatccag aaagtacgag
    13501 tttacagcga caagcaaaag agcaattgct tgcaggcgag cacattggct ctggcttagg
    13561 tattcttact tcataagttt agtacttcga aatagtttaa aagagcgtga gacaaaagta
    13621 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct
    13681 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    13741 ttcttggagt taaacgcttc tgtcccagcc tcttttttag tgttctatcg aacacacagt
    13801 acgttcaata ggtaaactag ctataacaat agatattaaa gttaacggac aagagggcct
    13861 tttttaggta gctaaaacgt aataatgaaa atttatttcc agagatagga gtcaatcagc
    13921 tagtctgatc gactgtttta acaagttgac tttcatttta acttttgata ccatttaaaa
    13981 cacaaatcgt aattgtttcg atttgtgttt tttagcatga ctacaatcaa agaagaaagt
    14041 gggtcgtcca tatggaaaaa acagatatct caagtgcata ccgccgcttg aaaagcccaa
    14101 atatcaaaac acgaaaacgt gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga
    14161 aaaggtaaga agaaggaaac ttaattaaaa aaatagccga aaaacgtttt tcggctattt
    14221 tcaataatcg gtttttgtgt aaggtttccg aaagaactgc tgttgatttt ccatttttaa
    14281 catatgataa attggtagaa tctttctttt caaattgata ccattcccta attccagtta
    14341 tatagttaac tacagaacca caaagcacgt acgtttataa aagtattatc tgtagttatt
    14401 tttcaattcc ctttcaacct cttattttga ccagtagtat tgatcacttt accttttaac
    14461 acctttgttc gtttttgaaa catttttagt caagaatgga aacgtaacta cataatcaaa
    14521 ggttttgcga aatatttcat gaaatatttc atgtagaagc gagcaatacg gttgcaaata
    14581 accattatcg tttaataagc agttacgaaa gctcccaata tctctaacgt tcagctcact
    14641 ccatttcttt tcttcaaaaa aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt
    14701 aactttaaaa agtaacgccg aaaaaatatg caaaaaaata aaaaagcagt tactattgct
    14761 agaataacat caaggaataa taagttttgg ttttcagata atagtcgata agttgtgtat
    14821 tggaatttta gatacagtaa taaagtattt ttcatgaaat tttgtgagaa tcactattac
    14881 aagtatcttt aataaaatta atggttaagg aggaacttaa agtagaatgt aaatgaatag
    14941 ttgtagtcag tcgattgtga tactagactc aaagttgcag gaataagttt ttcaatttgt
    15001 gattggaaga taaaaaatga aaaagaaggg ctttttttta tactttgttg attgtagtgt
    15061 ttattttccc tctgtttagc caagtaacat tttctatgac tcaaccagtt tcaagtgagg
    15121 aacatgataa cctttttcgt gctacagaag agtttaagaa atatcaggaa aatattgttc
    15181 cggagactcc attagctagt gagccaatta ctgatattga tggaaaccaa ataggatggt
    15241 atatacagta cgaagttatg gtggtaaata atgactcaat agtaaaaaat acagtaggct
    15301 tagagctaac accgattgaa aaggtgagtt cattgttaac gtatatttat actgatgaaa
    15361 gtgacgagac tgagatcatt ttaacggatt actcaaaaat tgctactgat aaaaagtata
    15421 tattgtggat gtagacacta gtgaaatact ttatgatata gaaattgatg aaaatttact
    15481 agatgtagca caagaaatcg atcatcaaaa gcaggaagct attcgatatg ctggtagaaa
    15541 agggaaaaga aaatatttat tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt
    15601 ggaaagaatt caagagtcgc tactcaatta actcgactcc gacatttaca gtttacagag
    15661 aaggatcgat agaaaaaact gttttttgga ctaaagaaag tggaatgtct ttagctgaag
    15721 tagaagagtt tttggattat gttagtatgc aacagtaagg gagttcttat tatgaaaaat
    15781 tttgacacat attatgttat tgttttttcg atatctttgc tgttaatatt actaacttta
    15841 atgcagtata aagagtatcc agagtatttt attttaactt ttgtatattt agtaagtagc
    15901 tacacacttc tatataaatt cagaagttct tctagagttt ctaaagtttg tttcttaatt
    15961 agcttattga ttctataata tttttgattt atataggaaa ttaaaaatat tatagaatca
    16021 acactcagag aacgggttgg tttttaatag atttaataag tactgataat gaaatttctc
    16081 ctggttggga aaacgaagtt ggttaccctg tagaagaatt ttttcaagat tactttgcgg
    16141 agtatgccga taggataatg agagattacg taacagataa aactcaagaa gtcatcaatc
    16201 aatcccagtc attaaatgaa ttatctgata aggcgaaaga agaagcagtt gccattattt
    16261 cttcagggat tagtgactat attgatatga gtggtgaaat caattatggc gataaagaca
    16321 aaattgtacg catgataaaa gcttttatca aaacttttga tgtgaaggca gatcttaagt
    16381 ttaatgatac ttacctagta ggaaaattaa ttaatatcct tgggacagag aaagatacgg
    16441 aaacgtttat ttcctctttg tctatgttgt ttacagataa gcatttttca actcagcttg
    16501 gtggagagga actagtggaa atcttttaac cattacgccc agcattaata aaagtatatc
    16561 aaaaaagtac tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa aacactattt
    16621 ttaatgttaa ttatagatag agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa
    16681 agatacactg ctatatttca ctaaggatat agcagtgtat ctttcatgtc gtttcactgg
    16741 cagtttttta taaaaggagg tgtgcatttc agttgctttt ctttggtagt tagttgaatc
    16801 gtttaagaat taacggatgt ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg
    16861 atggttccat ctttatttag ctgtgcctgt tcaaatactg ttttgttttt accgaatgga
    16921 tcaattgcaa taattgttcc gatccccgca cgtttggcag caagtaatcc tgagtaagca
    16981 tcttcgataa caagacaatc agccggagct aagctcagtt tgtctgccgc tttaagaaaa
    17041 atatccggtt gcggtttccc agggaaagag ccgtcgtcat agacaatctt gtcccagtca
    17101 aaccagcggg ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt gacagtagcc
    17161 gtggcaatcg ttaatggaat ctgttggtca gtcagctggt caagagtcgc ttcaagccct
    17221 tttgtcaact gaagctcgtt ttcattttgc aagcaaagca tacgataata gctttctttt
    17281 tcttctgaca atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa
    17341 atctccgtat tcgtccgacc atgaatattt tttaaaatat cctcgtctga taaaccatct
    17401 tttgtatgat catgtatcat atgcagccaa gcagcttcat gcagatgaga gtccaaaaac
    17461 attgttccgt tgaaatcaaa tataatccct ttcatagtta cgctcctata gtggtatttt
    17521 cggttgaatc agcaatttgc agatcgacat attgtgcata gtgctcttga acctcccgcg
    17581 agatctggcg atccgtaatg agcagatcga aatcatgcaa attataaaac acataaaaat
    17641 catctcgatt gaatttttta ttatcaacga gtagatattt cttacgggaa ttatttagag
    17701 cgatctgctg tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc
    17761 catttgaact gataaatgct tttgtaaact ttagtcttct taaattgtcg ttggtgatcg
    17821 gtccgacaaa tgcgccggta tttttccgga aatcaccgcc aatcagtaga atttctggtc
    17881 catctgcttg agcaagaata tcaaaaactg gataattatt tgtaatgatc cgaattttct
    17941 tgttcagtaa atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag aaaatcgttt
    18001 ctccctcgtg aatcaaggag gctgctgtgg cagcaatttc ttttttttct tgaatctgca
    18061 gagtggcttt ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga ctttgagccc
    18121 ctccatggac acgcagcagc ttgcccgatt tttctaattc atctaaatct ctgcgaacgg
    18181 tcatatcaga aacatctaat tgattgatga tttcattaac ggtaaggatt ccttttttat
    18241 tgaccatttc tacgatagta agcaatcttt cttttttcaa cattgtcttc acctctaatt
    18301 taaggataat ctatcatata aacggacaac agtcaatcta attcgaacaa aagtaaacat
    18361 ttagggtggt ttttatgttt gttacaatag attttattgc tgttttctgg tttttatgat
    18421 tatttgtgtt tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca atcagttaga
    18481 aagagcgaaa actgattata gatttgagga gagatgtaca tgcttaaatt accagaagat
    18541 tttatttttg gcggagccac agcagcctat caggttgaag gagccactaa ggaaggtggc
    18601 aaaggcgctg ttgcttggga tgacttttta gaggaacaag ggcgattcag tcccgatccg
    18661 gcaagtgatt tttatcatca atatgcaaag gacattgaat tgtgtgaacg ttttggtgta
    18721 aatggtctaa gattatccat tgcttggagc cgcatttttc ctgatggagc tgggaaacca
    18781 aatccagaag gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta
    18841 acgccgtttg tgacgttgca tcattttgat acccctaaac gtttgtttga ccatggagat
    18901 tttttgaatc gagaaacaat agaagcattt gtttcctatg ccattttttg ttttcatgaa
    18961 ttcaaagaag tcaaggtgtg gagtaccttt aatgaaatct atccagttgc aaccaatcaa
    19021 tatttgttag gtgttttccc accaggaatc aaatatgatt ttacaaagat cgtcgcctgt
    19081 cttcataata tgatggttgc tcatgcccgt gtagtgaact atttcaaaga aaacgaactt
    19141 ccgggtgaaa ttggtgtagt tcactcatta gagacgaaat atgcggcaac agacgcgccg
    19201 gaagataagc atgctgcctt tttagacgat gcattgtcga tccgtttctt attagatgct
    19261 acctatttag gctattattc aactgaaacc ttgactgctc ttgatgagat atgtgaagca
    19321 aatcaggcca gctatcattt tcctgaagaa gatttcgttg aattgaaaaa agcatccacg
    19381 cgtaatgact acttagggat caatcactac caatgccatt ttgtcaaagc ttacgatgga
    19441 gaaaatgcaa tccatcataa cggtacgggt gaaaaaggga cctctgttta taaagtgaag
    19501 ggaatcggag aacgtattta taaagaagga attcctcgaa ctgattggga ctggctgatt
    19561 tatccagaag gtctatatga cttgttgctg cgaattaaaa gcgattaccc acattacaac
    19621 aaaatctata tcactgagaa cgggatgggg tataaagatc agtttgaaga tggcattatt
    19681 atggatcagc cgagaatcga ctatttgaga gtctatttgg aatccttaag caaggcgata
    19741 acagccgggg taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct tttctcatgg
    19801 acaaatgggt ataacaaaag gtatggcttg ttttatgttg attttgaaac ccagaagcgt
    19861 tacccaaaag aatcagctta ctggtacaag ttggtcagtg aaaccaaaac gattatttaa
    19921 taagtgataa tggaggaaga aaagatgaat aaagaggaat tacaaatgct tggttttgag
    19981 atcgttgcat attcaggcga cgctcgcagt acattattga aattattgaa agaagtaaga
    20041 caaggaaact ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt
    20101 gcacataatt cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga gatggagatg
    20161 ggattcattt ttatccatgg gcaggatcat ttaatgacca ctttattgct gagagatctg
    20221 attcaagact ttattgtact gtacagacag aatcaaggat aggagaagaa cgctatggat
    20281 ggaatcatta aacaaatcga aaaagggaaa ccattttttg aaaaaatctc acgtaatagt
    20341 tatttaggag ccgttcgtga cggattttta acagcgatgc cagctatttt attttcaagc
    20401 attttcattt tgattgctgc ggttcctgag atttttggtt accaatggcc tgaatctgtc
    20461 tccacatggc tatggaaagt ctataattat tcgatgggtg ttgtagggtt actcgtttca
    20521 gcaaccactg ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta
    20581 atcaacacca cctctgtgat gctggcctct gtggtaagtt tcctgttgtt aagcgtctct
    20641 ccgattgaag gtggattttc aacggaatat atggggacaa agggactact ggcttccttt
    20701 gtcgctgcat ttttaacagt taatttatat aagttttgtg tgctgaaaga tattaccatt
    20761 aaaatgccta aagaagttcc tggaacgatt tcgcaaacct tccgagatat ttttcctttc
    20821 tctttttcag tatttgcggc cgttttaatt gatttaggca ttcgacaaat cttccaaatc
    20881 tcatttgcag aagcaatcat tacggtgctg cagccgctat ttacagcagc tgacgggtac
    20941 ttaggaatcg ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga
    21001 ccgtcgatcg tggagccggc aattgcggca attatctatg ccaatgtgga aaccaatctg
    21061 caattgttca attccggtga acaagcttct aaagtactga cggtgggttt agggaatttt
    21121 gtaggaacta tgggaggaac tggggcaaca ttggttgttc cttttatctt cctactcttt
    21181 gctaagtcta agcagttgaa ggcagttggg aaagcttcct ttattccagt aagtttcgct
    21241 gtcaacgaac cattgctatt tgcggcaccc atgattttga acccttattt ttttattcca
    21301 tttttgttaa cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc
    21361 atgaacagct ttatgtacgt tttgccttgg gcaactccag caccgatcgg tttggttcta
    21421 ggaacgggca tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc
    21481 ctgatctatc taccattttg caaagcatac gatgctgtct tagtagagaa agaagcacaa
    21541 aaagcagagg tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga ggaagtacct
    21601 gtggcagcga gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt
    21661 gctggcgcag ggacaagtgg gatgttagct aatgctttaa aaaaaggtgc atctgaatcg
    21721 aaagcaccga tcagtgcaat ggcgggtgct tatggttctc attacgaaat gctgcaggat
    21781 tttgatatgg tggtcttagc accgcaagtc aattcttact atgatgatat caaagaagac
    21841 accgatcgct taggaatcaa gctggttgcg acaaaaggag cagaatatat cggattaacg
    21901 agagatcctg aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag gaggagccat
    21961 aatgaaaatg ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt taggggcttt
    22021 gggggctgtt tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg gagcagatat
    22081 ttcgatcctg tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa
    22141 agatgcctta gagattttaa aaaataatgg tgtcaactat gttcgactgc gtttgtggca
    22201 ggatccttat aatagtaatg gagaaagtta tggagcaggg acaaatgatc tggaaacgac
    22261 gattgacctt gcaaaacgag caaaaaatct gggacttaag gtgctgctgg attttcacta
    22321 cagtgatttt tgggttgatc ctgggaaaca gaatcttcct aaagcatggc aaggtctgac
    22381 ctttgaagaa atgaatacgg cattgtacga ctatacaaag aacgttctgt cggaaatgaa
    22441 gcagctggac gtttatcctg atatggttca gatcggcaat gaactgaata gcggaatgct
    22501 ctggccttat ggcaagtctt ggggagaagg cgggggagaa tttgatcgat tggctgcttt
    22561 tttgaagtct ggtatccaag ctgttcgaga cactcaacct aaaacaactc cagtaatgct
    22621 ccacttagct gatggtggcg atacaggagc atttacttgg tggtttgatg aaatcacgag
    22681 tcgaggtgtg tcatttgatt taattggagt gtcgtattat ccgtattggc acggttcaat
    22741 gaatgaactt caaaccaata tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt
    22801 agttgaaaca gcttatgggc atacaacagc gaatgccgat actatgccaa atgcttttgg
    22861 tgaagccgaa gcagctgcgg ggggatatga accaacaccg acaggacaag cagagtattt
    22921 gctggatctt gccgatcgga ttcaggcagt accgaataat cgcggggcgg gcttcttcta
    22981 ttgggaacct ctttggtaca acggaaatgt gagttgggca acacaagcag gtatgaatta
    23041 tctcagtgtt caatccacta tgggaaacga atgggacaac caagcgatgt ttgattttgc
    23101 tggaaacgcc ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt
    23161 taaaaatgca agtttcgaaa gcgatggacc aacgaccagt ccttccaatt ggcaaacctg
    23221 gtttcaacaa ggaaccgcta agggtacgat caaaacggaa gcgggcactt tttttgggga
    23281 ctacaaacta actttttggc aggatcaagc atacgaggca tcagcttatc aaaatgttag
    23341 tggcataagc aaaggaactt acacattgag tgcctatgta atgtctgggg gagagtttga
    23401 tactaatcag ttatatatca aaaattatgg tggagcggaa ctaaatactg cggttgttaa
    23461 aacaagtgaa tggacaaaaa ttacaatcaa agacattcca attacaacag gtgtttgtga
    23521 aattggggtt tatagcaagg cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt
    23581 ggtgaaacaa tagactgcgg atcgtttcga ttcaagatcg gaggttccct tcagccacat
    23641 cgttagcgta gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa
    23701 cgatttccag tcatttttac caaaacgagt aaagcaccag acttcatcta cactttgtat
    23761 tatgctaaag agagttcaac gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg
    23821 cagataacat aaagtttgtt taagaaaggg taaccttaga attccgtcgt gaatatgatt
    23881 tgagcactta gatggataga aaaaagttct cggaaaacag ttcgtttttc gagaacttct
    23941 ttttatgata agaatgaaac aaattaactt atattatagt ctcttttggt agggaaccat
    24001 cttattttta atgaaaatca agcattttag ctattgaaga atgttgattt atagggattc
    24061 cccctccata ttatgttggc tctttattcc cactccactg ttgctggtgg tttagaagtg
    24121 atgtcataca cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt
    24181 tgcaagactt cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca
    24241 gcacggatac cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg
    24301 atccctggaa gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt
    24361 tcttcacgca agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg
    24421 ccaagaacac gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca
    24481 ggcatgccta actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt
    24541 tcgatcaatt caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc
    24601 gtctgtgctg tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg
    24661 aaagagacgc cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat
    24721 tcattcccaa tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag
    24781 aagcgatctc ttgcattgac tttgatgatg ttcaagccga attttccgcc tagactttcc
    24841 attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat
    24901 tggtcaccga tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa
    24961 agaccaagca agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg
    25021 ataaagttat ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc
    25081 agcaattcga ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa
    25141 ttacgtgttg tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca
    25201 aagccttcag gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt
    25261 ggtgtttctt taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat
    25321 tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca
    25381 tagcaaatcc ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca
    25441 ttctcatcat atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct
    25501 tttacttctt ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga
    25561 atacgtcttg tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca
    25621 acgttcgtca tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc
    25681 tcaaaatagt caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat
    25741 atgattttca gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc
    25801 ctgtaaattt ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc
    25861 acgatctccg atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag
    25921 taacgcacca aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata
    25981 gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt
    26041 attgatcccc tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat
    26101 gatatcatac acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac
    26161 acggttcaag aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc
    26221 catgattcca cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag
    26281 ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt
    26341 actttttcca acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta
    26401 tttatgaaga aataaacctt tactcaagaa caaagattca aattctttca tataaagata
    26461 aatcagatga gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt
    26521 caaacttttg atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa
    26581 ttctcgccat tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc
    26641 ctgcgttctc ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta
    26701 agagagattg ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct
    26761 tgacttgcgc acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg
    26821 gaagacatga caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa
    26881 tgggcttttg agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca
    26941 cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta
    27001 cctgatgaag gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg
    27061 gcatttgcga cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta
    27121 gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc
    27181 tatgaagcag ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct
    27241 ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt
    27301 acactgacaa ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa
    27361 gaaacatttg gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt
    27421 gtgaaagaat aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat
    27481 gtacgggtac gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt
    27541 ttcttcaaaa agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat
    27601 acagaacttg tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca
    27661 tactttcctt ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag
    27721 atagaaggga gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt
    27781 atcagtaaca actgtctcac agattttgaa taacaaagga agcagattta gtgataagac
    27841 aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc
    27901 aaatgtgatc aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt
    27961 cttcttctcc aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat
    28021 catgttatgc aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc
    28081 ccaccgctca gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc
    28141 attagacagc ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg
    28201 tcgggataac ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct
    28261 gtttattgaa aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca
    28321 attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa
    28381 tgggaaattt gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa
    28441 attactcaaa cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg
    28501 atgctatcag gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg
    28561 attcgatgga ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc
    28621 aagttttgat attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca
    28681 aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt
    28741 attgacaaag ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta
    28801 gtgctttttt tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa
    28861 tgtataatac cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa
    28921 aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat
    28981 acgaatggaa agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa
    29041 gacatgacaa atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc
    29101 atcgatttga gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt
    29161 gacacgacca cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa
    29221 atgtctggac gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc
    29281 ggatttcaga tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta
    29341 gcggtaattg gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt
    29401 gacgtcacag cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa
    29461 atcgcggcag gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg
    29521 aagaacatcg aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct
    29581 aaccgagaaa cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga
    29641 aatagtctag aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta
    29701 gtagaaatgt gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca
    29761 accgatgaag caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa
    29821 ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga
    29881 attttgactg cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa
    29941 atcgtggcaa atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa
    30001 gaagaagata ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg
    30061 acaaaaggtg agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt
    30121 gaattattat ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat
    30181 gacatcatca cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa
    30241 gaaatggaac taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa
    30301 gctgtgatgc aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat
    30361 ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca
    30421 gtaatcgggt ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat
    30481 gccatcaata acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca
    30541 aaggatgatc aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt
    30601 gcgcttgtca aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct
    30661 tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca
    30721 ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt
    30781 gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt
    30841 gctacaagaa tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc
    30901 tattaatttt ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag
    30961 ctattgaagc gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc
    31021 tcgtaacgga atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat
    31081 taacgaattg tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat
    31141 ttacgcatct agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc
    31201 gacaagtaat ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg
    31261 gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc
    31321 tataactcat gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg
    31381 ctttacagaa atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg
    31441 gttattttta ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc
    31501 atcagacaca acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt
    31561 caaccagtca gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg
    31621 aaatgatggc ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga
    31681 ccaagcattg aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc
    31741 gatccaagaa caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat
    31801 cgatggatta gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc
    31861 aggtgttgca gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa
    31921 aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa
    31981 agaattagga aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga
    32041 taaaggacgt tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc
    32101 ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga
    32161 caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt
    32221 aaacggcgaa aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt
    32281 agaagatttg gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta
    32341 tggattgaaa gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa
    32401 agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc
    32461 tgaagaaaac taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt
    32521 ttgattagaa caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga
    32581 acaactccct gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag
    32641 tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata
    32701 ttgtcataga aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca
    32761 tcaatttaca agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca
    32821 aatctacatt gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga
    32881 tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga
    32941 tggtccatca gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg
    33001 gaagcgagcc tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa
    33061 aagtttctga acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag
    33121 ttggaatgga acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga
    33181 tttttgatga gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca
    33241 tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa
    33301 tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca
    33361 acgttaaaga cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat
    33421 tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag
    33481 tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg
    33541 gcgaagtact tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt
    33601 tgactggttt gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta
    33661 ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata
    33721 aatacgggtt agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc
    33781 aaaaacctta cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag
    33841 aattgatcga agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat
    33901 caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt
    33961 tgattgttgc aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac
    34021 gtctgatcga acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag
    34081 aaattttaaa tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg
    34141 ttgatccgaa agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag
    34201 aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa
    34261 ttccgtaatt tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata
    34321 ttgatggctg cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc
    34381 cttggttcgc aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca
    34441 gcattaggtt tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa
    34501 gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa
    34561 gttgttttgc ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct
    34621 gttccaggtt tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg
    34681 aactatatct tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat
    34741 cgctcaagtt tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg
    34801 ttaacacaag cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg
    34861 cttattttcg tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt
    34921 ggattgaatc cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg
    34981 tcgatggtca tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg
    35041 tatcaaaact tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt
    35101 tccttgcttg gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt
    35161 ttgaagatcg gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat
    35221 gtatcgattg ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg
    35281 gctaaagtct tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca
    35341 acgaatcaaa accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga
    35401 tccttacttc tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt
    35461 tttctgaacg aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt
    35521 ttagttccat cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga
    35581 tcgctatttt agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa
    35641 cagtcagttt gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg
    35701 ctttaggtgt tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc
    35761 agaatctggg gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt
    35821 tctttaaaaa tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg
    35881 tgatcatctt caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag
    35941 ctgccgacac attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg
    36001 gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag
    36061 ctggtacgat cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga
    36121 atcctttagg ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca
    36181 ttggcgcaca attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat
    36241 atttgctgac aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa
    36301 acgggaagaa ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg
    36361 gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg
    36421 aataatcatg tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc
    36481 accagatgcc gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga
    36541 agcaggattg aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga
    36601 aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt
    36661 agggaaagat acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt
    36721 tcgtgtgttt cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg
    36781 ccgtaaagtc gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga
    36841 acaccaaatg aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat
    36901 cgcggcacat gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg
    36961 tgatataaca aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg
    37021 tgaaccagga gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg
    37081 acacacagtc cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat
    37141 caacgatatt ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga
    37201 tggcgttgac cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa
    37261 tttagtagat tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc
    37321 gattgaagaa tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt
    37381 gctaatgatt acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg
    37441 agaatatgta ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca
    37501 aggatattat tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga
    37561 aaatggcgaa agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg
    37621 accaagttaa gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa
    37681 gaaattgatt ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa
    37741 gatgctgtaa tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg
    37801 catgcaggaa agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg
    37861 agattccact actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg
    37921 gccggactag gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca
    37981 tttgtaccag gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct
    38041 ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat
    38101 acgcctgact atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa
    38161 gaaggcgtat atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg
    38221 tctcggacaa taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca
    38281 gctcacagcg gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg
    38341 caagcgaatc tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt
    38401 aaagcattgg ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt
    38461 gcatattgaa gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt
    38521 gcgagctaaa tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg
    38581 cggtatgtta ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg
    38641 aatgggaatg ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa
    38701 gaaattgatt cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt
    38761 agtactagca caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt
    38821 tcattttcct ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga
    38881 aaagggctat acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga
    38941 tttgacagag acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc
    39001 ggctctttat tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag
    39061 cgaccatttg atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga
    39121 aatgatcgaa gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc
    39181 cctgcattaa gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct
    39241 ggacttatta ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc
    39301 cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata
    39361 ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt
    39421 gacaaatcgt gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa
    39481 ctgttaagtc atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca
    39541 tgatgtcttc gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat
    39601 gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga
    39661 atggcaaagc gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt
    39721 ctaacattgc gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg
    39781 gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag
    39841 ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg
    39901 atttgatttg tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga
    39961 ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt
    40021 tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac
    40081 cagtaaattg gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg
    40141 gcatgtttag ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta
    40201 ttgtacaatt ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac
    40261 aagtagagaa tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga
    40321 tgcataattg ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg
    40381 aaacaccgca ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc
    40441 ggattttttt gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac
    40501 gagcttcatc taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta
    40561 ggttttccat taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa
    40621 gactaatcaa gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg
    40681 tagatttgcc taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag
    40741 cgatcactga ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat
    40801 ggaaaatttc ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt
    40861 catttatggt tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat
    40921 tttctaactg gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt
    40981 cgcaaatttt ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac
    41041 ggattgcgtc aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt
    41101 ttaggaaaag cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt
    41161 ctggctgttc gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta
    41221 agacttctcc gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat
    41281 gaagtagctg ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt
    41341 tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta
    41401 ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt
    41461 catcagcacc gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta
    41521 ttcctaattt ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta
    41581 aaccttcttc acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg
    41641 tcccgagacc tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt
    41701 gttttaaatt catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta
    41761 ttttagcgtg atttacggta aaaagatacc attctttttt attccaataa agctttttca
    41821 aaaaaaattg catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct
    41881 ttatctacag gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg
    41941 atgcagacat tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta
    42001 gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa
    42061 acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag
    42121 ctttaagaaa actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt
    42181 gggtacaatg acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt
    42241 gtcaaactta attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca
    42301 cagtgttgta ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat
    42361 cgttcgcggt gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata
    42421 cggaactaaa aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact
    42481 atatagaagt aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa
    42541 ggtcctgttg caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact
    42601 cgcttgatca accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat
    42661 aatgcctttg acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa
    42721 gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac
    42781 taccaagtac cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta
    42841 gtaaactacg ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc
    42901 atggatgctg caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg
    42961 gctgatgcca accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc
    43021 ttttataagt cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga
    43081 gaaacaagga gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg
    43141 gcccacatcg atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa
    43201 atccataaaa ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa
    43261 caagaacgtg gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga
    43321 atcaacatca tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg
    43381 cgtgtacttg atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact
    43441 gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa
    43501 atggacaaga tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa
    43561 gcaaacgctc acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc
    43621 gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa
    43681 acagaaattc ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa
    43741 gctgtagctg aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact
    43801 gaagctgagt taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta
    43861 ctatgtggtt cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat
    43921 tacttgccat caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa
    43981 gaagtagaac gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg
    44041 actgacccat tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca
    44101 ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag
    44161 atgcatgcca actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct
    44221 gttggtttga aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt
    44281 cttgaatcca tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa
    44341 gctgaccaag ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc
    44401 cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac
    44461 ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg
    44521 cctcaagtat cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta
    44581 cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa
    44641 gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac
    44701 attccagctg ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat
    44761 ccattagtag atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat
    44821 gaaactgcct tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat
    44881 ccagtaatcc ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt
    44941 gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct
    45001 ggcggccaac aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca
    45061 actcttcgtt ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa
    45121 gatgtaccaa aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa
    45181 tttcttgaaa ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata
    45241 gtttcgtgta aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat
    45301 acctatcaga caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa
    45361 gaaaaatttg accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat
    45421 ggtaaaacta cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa
    45481 gctatggctt acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc
    45541 tcaacagctc acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca
    45601 ggacacgcgg attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc
    45661 ttggtagttt ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct
    45721 cgtcaagttg gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac
    45781 gaagaattac tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc
    45841 cctggtgacg atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct
    45901 tcatacgaag aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca
    45961 gaacgtgaca acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga
    46021 cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt
    46081 gaagttgttg gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc
    46141 cgtaaattgt tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt
    46201 gcacgtgaag acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat
    46261 acaaaattct ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca
    46321 ttcttcacta actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt
    46381 gaattaccag aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa
    46441 ttaatccacc caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt
    46501 acagttggtg ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa
    46561 agcaacattt attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc
    46621 ttttttattt
//
LOCUS       DACCWK010000014        45489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCWK010000014 DACCWK010000000
VERSION     DACCWK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.014"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(24..1043)
                     /locus_tag="IVW65_000689"
     CDS             complement(24..1043)
                     /locus_tag="IVW65_000689"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7899612.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="IVW65_000690"
     CDS             complement(1676..2680)
                     /locus_tag="IVW65_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7899613.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="IVW65_000691"
     CDS             complement(2693..3742)
                     /locus_tag="IVW65_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7899614.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFFFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="IVW65_000692"
     CDS             complement(3759..4580)
                     /locus_tag="IVW65_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7899615.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="IVW65_000693"
     CDS             complement(4567..5346)
                     /locus_tag="IVW65_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7899616.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="IVW65_000694"
     CDS             complement(5363..5833)
                     /locus_tag="IVW65_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7899617.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="IVW65_000695"
     CDS             complement(5845..6264)
                     /locus_tag="IVW65_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7899618.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9113)
                     /locus_tag="IVW65_000696"
     CDS             complement(6345..9113)
                     /locus_tag="IVW65_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7899619.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            9313..9537
                     /locus_tag="IVW65_000697"
     CDS             9313..9537
                     /locus_tag="IVW65_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899620.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10316..12031
                     /locus_tag="IVW65_000698"
     CDS             10316..12031
                     /locus_tag="IVW65_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899621.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12034..13797
                     /locus_tag="IVW65_000699"
     CDS             12034..13797
                     /locus_tag="IVW65_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899622.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(13837..14373)
                     /locus_tag="IVW65_000700"
     CDS             complement(13837..14373)
                     /locus_tag="IVW65_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7899623.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(14696..15118)
                     /gene="mgsA"
                     /locus_tag="IVW65_000701"
     CDS             complement(14696..15118)
                     /gene="mgsA"
                     /locus_tag="IVW65_000701"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7899624.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            15327..16436
                     /gene="ugpC"
                     /locus_tag="IVW65_000702"
     CDS             15327..16436
                     /gene="ugpC"
                     /locus_tag="IVW65_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7899625.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            complement(16560..17189)
                     /locus_tag="IVW65_000703"
     CDS             complement(16560..17189)
                     /locus_tag="IVW65_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899626.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17640..18524)
                     /gene="rsmA"
                     /locus_tag="IVW65_000704"
     CDS             complement(17640..18524)
                     /gene="rsmA"
                     /locus_tag="IVW65_000704"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7899627.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(18517..19086)
                     /gene="rnmV"
                     /locus_tag="IVW65_000705"
     CDS             complement(18517..19086)
                     /gene="rnmV"
                     /locus_tag="IVW65_000705"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7899628.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(19079..19855)
                     /locus_tag="IVW65_000706"
     CDS             complement(19079..19855)
                     /locus_tag="IVW65_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7899629.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(20010..21332)
                     /locus_tag="IVW65_000707"
     CDS             complement(20010..21332)
                     /locus_tag="IVW65_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7899630.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            21507..22094
                     /locus_tag="IVW65_000708"
     CDS             21507..22094
                     /locus_tag="IVW65_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899631.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(22573..24582)
                     /gene="metG"
                     /locus_tag="IVW65_000709"
     CDS             complement(22573..24582)
                     /gene="metG"
                     /locus_tag="IVW65_000709"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7899632.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            24850..25128
                     /locus_tag="IVW65_000710"
     CDS             24850..25128
                     /locus_tag="IVW65_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899633.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            25225..26046
                     /locus_tag="IVW65_000711"
     CDS             25225..26046
                     /locus_tag="IVW65_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7899634.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(26331..27341)
                     /locus_tag="IVW65_000712"
     CDS             complement(26331..27341)
                     /locus_tag="IVW65_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7899635.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSAMISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(27353..28306)
                     /locus_tag="IVW65_000713"
     CDS             complement(27353..28306)
                     /locus_tag="IVW65_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7899636.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(28296..29366)
                     /locus_tag="IVW65_000714"
     CDS             complement(28296..29366)
                     /locus_tag="IVW65_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899637.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(29372..30415)
                     /locus_tag="IVW65_000715"
     CDS             complement(29372..30415)
                     /locus_tag="IVW65_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899638.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(30416..31357)
                     /locus_tag="IVW65_000716"
     CDS             complement(30416..31357)
                     /locus_tag="IVW65_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899639.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            31441..31788
                     /locus_tag="IVW65_000717"
     CDS             31441..31788
                     /locus_tag="IVW65_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7899640.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(31858..33519)
                     /locus_tag="IVW65_000718"
     CDS             complement(31858..33519)
                     /locus_tag="IVW65_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7899641.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34065..34613)
                     /gene="aac(6')"
                     /locus_tag="IVW65_000719"
     CDS             complement(34065..34613)
                     /gene="aac(6')"
                     /locus_tag="IVW65_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7899642.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            35166..36269
                     /locus_tag="IVW65_000720"
     CDS             35166..36269
                     /locus_tag="IVW65_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7899643.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(36462..37529)
                     /gene="rlmN"
                     /locus_tag="IVW65_000721"
     CDS             complement(36462..37529)
                     /gene="rlmN"
                     /locus_tag="IVW65_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7899644.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(37759..40206)
                     /locus_tag="IVW65_000722"
     CDS             complement(37759..40206)
                     /locus_tag="IVW65_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7899645.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(40395..41831)
                     /locus_tag="IVW65_000723"
     CDS             complement(40395..41831)
                     /locus_tag="IVW65_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7899646.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            complement(42150..42965)
                     /locus_tag="IVW65_000724"
     CDS             complement(42150..42965)
                     /locus_tag="IVW65_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7899647.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            complement(42983..43618)
                     /locus_tag="IVW65_000725"
     CDS             complement(42983..43618)
                     /locus_tag="IVW65_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7899648.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            complement(43637..44278)
                     /locus_tag="IVW65_000726"
     CDS             complement(43637..44278)
                     /locus_tag="IVW65_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7899649.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            44758..45243
                     /locus_tag="IVW65_000727"
     CDS             44758..45243
                     /locus_tag="IVW65_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7899650.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
BASE COUNT        13194 a         9420 c         7956 g        14919 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaaaaaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgccagtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt ttttatgcag
    10081 aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga gactggcatg
    10141 ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag cttcttcata
    10201 ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc ctaagctaca
    10261 atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga atcatatgat
    10321 tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc cgttcttcaa
    10381 atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct tgatcgataa
    10441 tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga tggtcgctat
    10501 gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag tagcttcaca
    10561 aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt tctctcacaa
    10621 cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg acatcaatca
    10681 gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt ttttaagtat
    10741 cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt tcttagcatt
    10801 attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc ctctctacca
    10861 aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc ttagcggtgt
    10921 tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg ataaaagcac
    10981 aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat tatcacctgc
    11041 tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg ggatcaaagt
    11101 aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata tgaatcaaat
    11161 gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg ccgaagcttc
    11221 aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag ctgaggtcag
    11281 tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg acttccgtta
    11341 tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc gaaatacagt
    11401 ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat tgattccgcg
    11461 tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc gttcttggca
    11521 gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac tatttaccgg
    11581 gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag aatgttggga
    11641 agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg gtttagatac
    11701 aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac tgacgattgc
    11761 aagagctttg attgccaagc cagcattact gattttagat gactctttaa gtgcgttgga
    11821 ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca gtacagtgat
    11881 tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag tgatggatca
    11941 aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag cagaatatca
    12001 ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt atccttgcag
    12061 acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg gctagctgtg
    12121 atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat tggtcaaagt
    12181 atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca tttacttatt
    12241 ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca agtgttaagc
    12301 aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc tcatctgaat
    12361 cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag tcgatttaca
    12421 aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt attttctgga
    12481 gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact tctgacattg
    12541 gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc tcgtgcttca
    12601 caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt tgtgaccgaa
    12661 cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag tcaacagcaa
    12721 tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt ttcttcttcc
    12781 ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat tggtttagtt
    12841 ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat ttccagcttt
    12901 acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat cacgacacaa
    12961 ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca gccagaagaa
    13021 aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg aaaagtctct
    13081 tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga tttcaatttt
    13141 actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg aaaatccaca
    13201 cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac agtcgatgac
    13261 catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat ggtgttacaa
    13321 gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa tccggaagca
    13381 agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt tgtagaacgt
    13441 ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc agatggacaa
    13501 cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt gatcttagat
    13561 gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc cttcttagca
    13621 atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat caaaaatgcg
    13681 gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca tgatgagctg
    13741 cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc acaataaaaa
    13801 caagcagtct tctgggatgg gaagactgct tgttttttat ttagcgtgga tcgctaaact
    13861 tcttaaaatt tttttgtaag gaatactacg tcctacgtaa taaatggcaa ttgattgaat
    13921 cggtaaccat attactgttt ttaacaggcg gggcgcccaa acgacccagc tgaacaatgg
    13981 cacatggtac atcaacgcaa gccataatgg tgtcaaaaat aagttgatga tcagcgtgac
    14041 actaagcgta gccagaatgg catttttcca agtgatctct tttcgataga aaaagaatcc
    14101 gtagattgct ccttcaataa atgcattcaa tgtaaatcca ataaagaatg cagatttcga
    14161 gaagagggcc attcctacta aatcagctaa aactgcgcct atccctgacc aaaaaggacc
    14221 aaaaagaatg cccatgatca cttgtggaat aaatgtgaca ctgattttaa taaatggggt
    14281 ctcgattgct accagtcggg acaaagtgac catcaatgca atcaataagc ccataatagc
    14341 aatcatacgt gcgtctaacc ggtgtttctt catttttagc atctccttta agagctgcta
    14401 catcgtatca tgcagcgaat gcgaaaataa aaccgcgaac attacgtcct tcgtccaaga
    14461 ggcaacatcc catcctctta gcacttaacg ctttattttc tactctgtat tatttttggg
    14521 atcttttccc acagaaacta tagcatattt tcacatttac tgcaaatacg aaaaactaat
    14581 attattgttt atattatcca atacgttcgt attatatcgc atattatcag tttgtttagc
    14641 ttttatagct acatataggc gaacattaaa cgttattttt ccatacgctt atccatcaaa
    14701 atgaaagtgg atgcttgtct atttcatgga cgattcttcg aaaatctagg aagccggctt
    14761 ccagtcctct tagtagcacc tcagctgttc cgatattcgt agctaacgga atctcataga
    14821 catcagataa acggataagt gctgttacat ctggttcatg gggctgtgca gccagcgggt
    14881 ctcgaagaaa aatcaccaaa tctaactgat cctccgaaat catggctcca atttgctgat
    14941 ccccgcctaa cggtccggat ttaaaccgat ggatcggcaa gccggttgct tccgtgatcc
    15001 tctgcccagt cgtcccggta gcaaaaagtt catgtttttc caatattgtt ttatacgctg
    15061 ttgctaattt caccatcata ggtttttttc ggtcatgagc gatcaaagcg attttcataa
    15121 gagacactcc ttttgatatt gttaacatac ttatttactc cagtgtatca gtttttattt
    15181 cttatggata tttattaaag cttaaaacta gcacattgac aaaaaaaaga aaaaattctt
    15241 gttcaacatg acattgaagt tagttaacgc tttcatttat actttaaata gttaataaaa
    15301 ctaaaaaaga aagtgtggaa cgtattatgg tagaaatggc tctgaaaaac gtatataaaa
    15361 aatatgacaa tgcagaaaat tattctgtaa ctgattttaa tctatcaatc aaagaccgtg
    15421 aatttatcgt ttttgttggt ccttccggtt gtggaaagtc aactactttg cgaatgatcg
    15481 cgggactgga agatatctct gaaggagaac taaatatcgg cggaaaagtc atgaatgacg
    15541 tggcacctaa agaccgtgat attgccatgg ttttccaaaa ctacgcattg tatcctcata
    15601 tgactgtttt tgataatatg gcttttggct tgaaattacg taaatatgat aaagcagaca
    15661 tcaaaaaacg tgtggacaat gcagcagaga tcttaggtct gacagaatac ttgaaacgaa
    15721 aaccagctgc tttatctggt ggacaacgcc aacgtgtggc attgggacgg gcaatcgttc
    15781 gggatgcaaa agtcttctta atggatgagc cattatcaaa tttagatgct aaattacgtg
    15841 tagccatgcg tgctgagatt gccaaacttc accagcgtct tgaaacaacg accatctatg
    15901 ttactcacga tcagacagaa gcaatgacta tggctgaccg aatcgttatc atgaaagacg
    15961 gatttatcca acaaatcggt acaccgaaag aagtttatga tacacccgtc aatatgttcg
    16021 ttgccggctt tattggatct cctgccatga acttctttaa tgtgacattg aaaaatggtg
    16081 ttatcacaga tggacatggg cttaagcttc gtattcctga aggaaaaaac aaagtattag
    16141 ttgaaaaagg ttatgaagga aaagaagtta tcttcggtat tcgtccagaa gacatccaca
    16201 gtgaacaagt agcccttgaa gcttcacctg aagccgtagt gaaatcagaa gttgtcgttt
    16261 ccgaattact tggagctgaa acgatgcttt acacaaaaac tggttctact gagttcatct
    16321 caaaagtaga tgcaagagat ttccataaac caggcgaaat agtcgacctt gccttcaaca
    16381 tcagcaaagg ccacttcttt gataaagaaa cagaacaagt catcaaactt ccataaattg
    16441 ccccctgtta gagctgtgcc agaagatgtt ggtgcagctc tttttaggac aaaaaactct
    16501 catcttagag gatttattat ccattactag atgagagttt tttttttgag agatcatgtc
    16561 taatatgtcg ttttcttcgc gtacccttga atcgactctt tccgattttc tttttttgct
    16621 gccgtaaata cggggatatc aaaatatttg ctgagtgttc ctgctataaa ataagcaata
    16681 ttataatcaa ctaaactgtg gataagccca ccaatcccca tcaagatgaa tactgtataa
    16741 aaatatcctt gagaataata tgtttcaggc atattaccaa taaagtaaaa gatgctgact
    16801 actagcattt ctgctaaaga atgaatcaca ccgatcacaa aattgaaccc ttggaaacgc
    16861 caattagcaa aagtaaactg tccatttttt aaaacgatac tcggatgctt ttgcaagtac
    16921 caagccccaa tcaacgcaaa cacaagatgt gacaatgctc gcaatgcgat gataaccggc
    16981 gttgttaaaa agaaaccaaa tgtcgtacct agcgcaacag ccactgcaac tcctggtgta
    17041 aaaaacatcg caataaataa tggcacatga gacgccaaag taaaggaagc tggtggaatc
    17101 atgattttag gcatgaccat tgggataatg acccccatcc caatcaataa agcagaaatc
    17161 gttagttttc gaactgaatt ttttctcatt ttttctctct cctaatatgt cttgacacat
    17221 aaatgtcgac ttagtataac tcacttacta ataactgtca agacacatat tcttttctaa
    17281 atccatacaa aatgagtgtg agtatcaagt ataagaattt tttgatcaag aaacaaaaat
    17341 aggagatgtg acaaaatttg tgtcacatct ccttgttcgg cataatagtg gtcaaaagct
    17401 tgcatcaagt aataagacca actaactcaa aaacatgaga agctgttttt tgaaattgtt
    17461 ccttgtgact tagagttaaa cggcaggccc acaacctctt tcttaaacgg tgttcaaaag
    17521 cttgcatctg cggtaataag cccaaacaaa cgaaaaacat gaggaactat ttttgtttgg
    17581 ctgttcttat tacttgatgc aggacagctt tttcacaact tctgtcacaa cctctttttt
    17641 tactgcatat tttctgacat cgcattgctt aatgcggcga attcttgtaa tgacaaagtc
    17701 tccccacgac gttttggatc gattccagct gtttccaagc tttttgctaa ccatgctttt
    17761 gtctggtcat ctttgccata cgaatgctgc aagttattcc aaagcgtttt tcttctttgc
    17821 tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt ctgtcacttc gactgccggt
    17881 atatctcttc ttgtcagttt caaaatcgct gaatctacat ttggctgagg gacaaatact
    17941 gtttttggta cgatgaatgc taaactggct tccatataat attgaacagc aatcgataat
    18001 gaaccataag ctttcgttcc tggttcagca gagatacgat cagctacttc tttttgcatc
    18061 atgacgacta gttcatccac aaccaaatct gattcaagaa aatgcatcat gatcggtgtc
    18121 gtaatatagt aaggtaaatt cgctacgact tttaacggaa gctcctcatg aaatgtttct
    18181 ctaacagctg ttgacaaatc ggtctttaaa atatcttgat gtacaatagt cacattgtca
    18241 tagggttgca tcgtatcagc caatacaggg atcaaacgat catcgatctc aaatgctacg
    18301 acttgttttg catgcttggc taattgttcc gtcaatgcac cgattcctgg tccaacttcg
    18361 atcacgttcg tatgttcatc tatttgcgca gtttctacga ttttccttaa aatatttggt
    18421 tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa aaccatgttt caataaaatc
    18481 tcttttgttc tagagggtgt cgcaatttca cgatattcac tcatgttgtt cctccactat
    18541 tttcattgct tgggcgagtt ctttcggcgt aatacgaaac atcttcaaac gtttttccaa
    18601 ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct agtttttctc gtttttcttt
    18661 tgcttgtgcc ccagctatta aaccatagtc taaaaggtct tgtctagtga tttcagaaac
    18721 agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat gcttctaaaa tagcagcatc
    18781 acttgcatgc tcgacaccaa ggcttccctt tttctttggt ttagcttgtg tgcgcggcaa
    18841 aaaagcatgt tttgcctgag gaacagcttc cataatgatt tttcttattt tttctcctga
    18901 aaaatcagga tcggtaaaaa cgatcactcc acgagtctct tgggcatgtt caatttgcat
    18961 caggatctct tcattgattg cagaccctat tgtttctatc gtatctgcat caactacttc
    19021 ccgcagcctt cttgtatcgt cttttccttc aacgactata atctcttgga tcttcatctt
    19081 atccatgttc gcttagacca aataatcggt gcgcatttgt ccatgtttgg gcagccactt
    19141 cttctgtcgg aagattccga agttcagcaa tcttttctac tgtataacga gtatagcctg
    19201 gttcatttcg ctttcctcta aaaggaactg gtgccagata aggggcatct gtttccacaa
    19261 gtaatcgttc gattggtaca tttgctgcag cctcttggac gtctaatgct tttttgaaag
    19321 tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag aaatcgtttt gcccattcat
    19381 aatcaccact gaaactatgc atgattccac cgatatctcg aacatcttct tcttttagga
    19441 tttgatacgt gtcttcaagt gcttctctag tatgaatact gattggtagg ttcatttctt
    19501 ttgcgatagc aatctgtctg cgaaatactt tatcttggac ttctttcggg tcttccatcc
    19561 aatgataatc caagccaatt tcccctaatg ctacaacctt tggtaaagtc aattgttctt
    19621 gtaatttatt ttcgatttct ttcgtataac tgccagcctc tgtcggatgc cagccgataa
    19681 tactgtatat atttggatag tcatggctta attgcaaaga cttttcaatc gttggcgtat
    19741 cgaagcccac gaccgccatt ttcgttaccc ccatttcttg tgctcgctga atcgtttctg
    19801 gaatatcttc attgaactgt tcagcattta aatgggtatg agaatcaaat atcatgttta
    19861 tctctccttt tcattcttac tttagcacat aagcactact tgcagaagta gcaaaaatca
    19921 aaatagaaga acttgtttta ttgaaataga aaaagcacga agatctagag gacctagttc
    19981 gtactttttc tgtctgttgt tgaaatattt tactcttctg agcgtttttt ctttttttga
    20041 taatagactg ggatacctat caaggtaaca cctataccga tcaatgctaa tttcgtttgc
    20101 gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa tcgcaataat cggaacgatt
    20161 ggataaagtg gtactttata tggacgtggc aattccggcg ctttttttct taaaataaag
    20221 acaccgataa aaatcagtaa actgaatagc cacatcacaa aaatcaacat gtctgttaaa
    20281 aagtcgaaag aaccaaggct catcataata caagctactg ctaactggaa aaccgcagga
    20341 acataaggaa ccgtaaactt ctttgaaagc tttgtcagct gtttactgaa aggtagctcg
    20401 tcttctaaag ccatcgcata aggcacgcgg atcccagtta gtgtataacc gttcaatgca
    20461 ccgtataccg agatcaagat cccaatagtg accagctttc caccgattcc tccaaaaatg
    20521 acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa gatgatcaat cggcaaagtt
    20581 ttcaaaaaaa caaaattgat caatagataa acgactgtta caaaactcaa accaagaata
    20641 attgctttag gcaaatcttt ttccggccgt ttcatttccc ctgccattgc accgacacct
    20701 aaccagccgt cataagcaaa caacgttgct aacaacgcac tgctcaatcc ttcgacaaaa
    20761 gtaacatctt ttccagcttc gattggaaat aactggacag ttccttgtcc tggggtcaat
    20821 aatccccata tgacaatcac agcaattggt attaatttaa caatcaacgt cactgattgt
    20881 acagaagcac cgacttttgt ccctaacaaa ttcaaccctg tcacagaaac agcggtgatc
    20941 actgcaatca acaaaagata atcggtagat aattgtaata agttcgtcaa ttgtgtcgca
    21001 aagataatcg ataatgcagc aatattagca ggaaagtaaa tcaagctttg cgcccatcct
    21061 aataaaaagc tgggcaattt tccatacgca gcttcaatat attttacagc tccaccagtt
    21121 tccgggattg ctgtagctaa ttcagccact gtcaatccag cacagatcgt taagaaacca
    21181 cccaaaagcc aagctagcaa tgtcaaactt gtagactgag tactagcagt aacagcagca
    21241 gttttgaaga agacgcctgc accgatcacg gttcccataa cggtagctaa tgcgccaaac
    21301 agattgattt ctcgttttag tttgcctttc attcccatcc tctttcttct ttgaaatttg
    21361 gtataatttt cagtataact gagaatattt taatttacca ttcttttttc tccactttca
    21421 gtgaaaaact ccctcttaag acggatgtat ttctttttta tttttcgtat actaaaccta
    21481 ccttttccac gaaggaggaa cagtttatgg attttaagcg ttcttatccg aaattagcag
    21541 attatttatc caaccgggtc gatttttccc gtaaagaaca acagcagtcc cattggcatt
    21601 ggttcacatt aattttattt ggattaatga ctgttgcaac gttatcacaa aaactaatat
    21661 tgctgacttt gttcatcgca atcgttgcat tgatcaaagg tcctgtgatg atcttgtggg
    21721 ggatgttgta ttcctttttg gttgggcttt ttccgcctat cggtattttg ctttcagcga
    21781 ttttcttttt attgaatatc ggaacattta caaagaactg gcggatgacc ttagtcggga
    21841 tctattttta tttctatcct tttggagtga tggcacttag tgaagtcatg cattgggata
    21901 atcattggtt tatagctggc tccttgcttt tagggttgat cttgttacac gtgatgttga
    21961 caaagctcta tcaacattat ggtatcggac gtacgatttt ctggtatgtc ttttcgatcc
    22021 catttgcact gcttactgct ctcttaccaa gccgtttgaa gacaaaaata aaaggttacc
    22081 ataaaatcaa gtgataggat ttttacaaaa taaagaaaac aatcatcgaa gtcaaagccg
    22141 atgattgttt ttttatctat attttcaatc acacatataa gagcacaaac taattgaaca
    22201 taaagtgata gaacttataa ttaacacatc aacgctactg ttagatataa atatagatgt
    22261 aataaagtca ttgcattcaa actttatgaa tgtaaggaca ataaaaaaga gtaccagatg
    22321 aatcaattaa aatctgcccg accctttatc attatgcttt ttttctgttt ttacgattcc
    22381 tattagtatt attctaaatt aacaaaaatc agcgaagctc gtacttcttt ttctaggatt
    22441 tattttggat agttctctgt ttctgtatta tatgattggc ttcaagtaat aagaaggaat
    22501 ttacgaaaat tgcttctcaa atttcggaaa aagggggtat gacacaacta gtgtcatacc
    22561 ccctttgaat ttctatgcga tttctgagcc atttggcatg gattttggtg cttcgattac
    22621 ttgtaaagag ccatctggtg cttctgcaga aaggatcatt ccttgactga tttgtccacg
    22681 catctttcgt ggtttcagat ttgctacaat gacgactttt ttcccgatca attcgcttgg
    22741 atcaggataa aattcagcga ttcctgacaa gatttgacgg tcttggctgt ctccagcatc
    22801 tagtcggaat tgcagcaact tatctgcccc ttctactttt tgacagttga tgacttctgc
    22861 tacttttagt tctacttttt caaatacatc aaatttgatt tgtttttctt tcgttgaaac
    22921 aagttctgtt tcttctggat cccatttgac tgtatcttcg tttgtttgag taccttcaga
    22981 cattttttct tgaataaaag caacttcttc ttccatatct aaacgtggga agattggtgt
    23041 accttttgca actacttttg ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt
    23101 cattgtttca gagtccaatc ctagttgatt gaagatcttc tctggcgttt ctgtcatgat
    23161 tggctgaagt aatatcgctg cgatacgcaa agattcagct aaatggatca tgacactatt
    23221 caattcagct ttcttttctt cgtcttttgc taatacccat ggttctgttt catcaatgta
    23281 tttgtttgca cgagaaatca gcacccagat ttcagccaat gctgtattga attccatttt
    23341 ttccattgca tcatggtaac gtccgacaac gttagcagcc gtcgtagaca gttcactatc
    23401 aaatggagtg acaagtgagg catagtcagg aacgagtcca tcacaatatt tgttgatcat
    23461 agcaatcgtt cgattcaata agttccctag atcatttgcc agatcataat tgacacggga
    23521 aacaaaatct tctggcgtaa agacaccatc tgacccaaac ggaacagcac gaagcaaata
    23581 ataacggagt gcatctaaac cataacgttc cacaagcatt tcaggataaa cgacatttcc
    23641 ttttgattta gacatttttc cgtctttcat caatagccag ccatgcccaa atactttttt
    23701 aggtagaggc aaatcaaggg ccatcagcat gattggccaa tagatcgtat gaaagcgaac
    23761 gatttcttta ccgaccatct gaacatctgc aggccagtat ttttcgaata gactttcatc
    23821 ttcggagcca tatcctagtg cagtaatata attggataat gcatcgatcc atacatacac
    23881 cacatgtttc ggatcactct tgactggtac tccccaagta aaagttgttc gtgaaactgc
    23941 aagatcctct aatcctggtt tgataaagtt gttgatcatt tcgtttttac gagattctgg
    24001 ctggatgaat tcgggatgtt cttcatagta ttccaataga cggtctgcgt atttgctcat
    24061 gcggaagaag tatgattctt cttttaccaa ttctacttcg tgtccacttg gcgctttacc
    24121 accgatcacg ttaccttcgt catctttgta cacttctgca agttgtgttt ctgtaaagaa
    24181 ttcttcgtca gaaacagagt accagccttc atattcacct aagtaaatat ctccctgttc
    24241 taataaacga tcaaatattt tttgaacagc tgctttatgg taatcatctg tcgtgcgaat
    24301 gaatttatca taactgatat caagcgtttt ccataatttc ttgacatcag ccgccatttt
    24361 atctacatat tcttgcggtt ttacacctag ttcttctgct tttgtttcga ttttttgacc
    24421 atgttcatcc actccagtga gataaaacac atcaaagccc atcaaccgtt tataacgagc
    24481 aattgcgtca catgcaatcg tagtatacga gttgccgata tgaagtttgc cgcttggata
    24541 atagattggc gtagtgatat aaaaagtttc cttttctgcc acggggctct tcctccttta
    24601 tcgcatgatg cgaattcgtt tccttgctag tataccataa aaaaagcatg ctgttagctt
    24661 tttttcaaaa tgaaaaagac tggataaacg acttctatct acgttcaatc tatcctttaa
    24721 aaggatgaat aggttgaata aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg
    24781 gctttttatt tctcccctca cacgtggtat aataaagact atctatctta gtaagggact
    24841 ggaacaatca tgcagaagaa acatcttttt tttacactta gtattgcttt tctttcatta
    24901 gcacatttaa tattcagcta tttttatatt cgtatgtatg gttattttaa cctccatggg
    24961 catttgaaca gttttatgac agcagcttgg attttgcgtt ttatcattga cgtatatatc
    25021 gttatttgtg gcttctttgc cattcgtgaa gagcggtata aagtattgcc gttttatctg
    25081 cttttctttt tattcaatct catacttcca tttatttttc atatttaaat gataacctag
    25141 atttttcaag taaatccttg gttttttttc agagtctgct acaataaggt aaaaagtact
    25201 tttactgtca atggaggtgg tcagatgaac cgttttgctt ctaaagaaga agaaaaatta
    25261 tattatgaac ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt
    25321 ccagaatatg acaagccctc tttgactgtc gatatcgtac tgatgtgcta taacaaagaa
    25381 gaagatcagc tgaaagtttt gttgatccaa agaaaagggc atccttatcg aaattcatgg
    25441 gcattgcctg gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa
    25501 acaaaggaag aaactggtgt ggttatttca aaagaaaata ttgagcaact gcatacattc
    25561 agtacgccta atcgagatcc gcgaggctgg gtcgtcacag tcagctactt agcctttatt
    25621 ggagaagaac cgctaatcgc cggagatgat gccaaggaag tacgctggtt cacactagaa
    25681 cgtcatagta acaagatcca tttatctagc ggagaagtgg ctatctcgct tgatttagtc
    25741 actggagagt cttctggcaa agatactttg gcttttgacc atagtcaaat catcttgaaa
    25801 gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc aagttctaca agttttaggt
    25861 aaagatttca cgattacgga agctcgaaaa gtatttgcta aattcttagg gatcgattat
    25921 aaaacgatcg atcattccaa ttttaagaaa gctatgctcc aatatttcga tgagatcggt
    25981 gaacgtcctg taggaatcgg ccgtccttca aaaatctatc agctcaagcc ggggcaccat
    26041 gaataaggtt gtgagcaaaa gattcagacc taatcattca agaaaaaacg caaaaacaac
    26101 tctttgtgag ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa
    26161 aaaattaaca atagcagaaa aagctcgtac gaactttttc tgctattgtt ttattggttt
    26221 gtcataatta gtgagctttt cttcaaaaga aaaattccta gaaacaaaag tcattaagac
    26281 tttttattaa caaggctaac cctaacgctc ctatcagttt cattcatttt ttatcccttg
    26341 ataaccgaat gtctgcccat tttttctact acttggctct ttcttacgga attgctttct
    26401 tctgctccgt cggtattgat caaaagaaat tttttattat accattggga cttataacgc
    26461 aattttgtta ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt
    26521 ggaaaagatt ctacaaataa gtagtcatat tgtttgacca atttcccaga taattgttca
    26581 agataatctt ctcgctggtt cttttgtttc agataaagat ccattacttt tctcttttgt
    26641 ttctgataat tgcttgcctg atctaacctg atctttctat ggtgagcgga gcggactttc
    26701 acattcaatt tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa
    26761 gttacttcta gaggtcgaga tgtttggatc aatgtcttcg gatgataggc gatcccgatc
    26821 catttagcta aagattcttt ttttactggc gaaacatcac aaagtagtgc gacataaaat
    26881 tcttcattat atcgagcaga aatcatagcg gagcgaatcg atccttcaac tggccgatgc
    26941 tgatttatct tgattagttc tttttgtttt ggtaacttta agtagccatt ttccagatag
    27001 atcgtacggg tttggttgtt ggtcgtataa gattgccaca tacttttttt cgttttaagc
    27061 tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa attaagctgt
    27121 gcatttgcta aagcaagact gtctacttct tttaagaatg ggtattcttt ttttaatgta
    27181 gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc aacttttctg ccgctttttc
    27241 agcaaatggt tataggtaaa tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt
    27301 tcttctgtcg gatacatacg gaatttaaaa gcttttagca tctatgtccc tcctagttag
    27361 caaccagaag agattccttt tgtcgtttta gtttttttgc gtaatatggg acttcctgct
    27421 cagttgcata gacaaaatgt tctggtgcga tttcacgaag ttgacgtact tgtccgtcat
    27481 ctggttcagg ttgatatttg attcttctgc gttttctctc atggtctgga tctggtagag
    27541 gaatagcaga taacaaactt tctgtataag gatgaacacc gtgattatag atttcatcac
    27601 ttgttcccat ctctaataac aagccgttat gcattacacc gattcggtca ctaatatgtt
    27661 taaccatcga caaatcatgt gcaataaata gataggtcaa ttgatgttct ttttggagat
    27721 cttgcaacaa attgaccact tgggcttgga tcgacacatc tagtgcagaa attggttcat
    27781 cacaaatgat aaaacgcggt tttactgcca atgctcgtgc aataccaata cgctggcgtt
    27841 gcccacctga aaattcatga ggataacgag taccgtggct tggattcaat ccaacagttc
    27901 tcaatagatc atcgactttt tttgcacgct cttcagatga tttagctagt ccattaacat
    27961 caattccttc ggcaatgata tctctgactt tcatacgagg attcaacgaa gcgtatggat
    28021 cttgaaagat catctgaaca tcatgccgga atttcgtcaa acctttttta tctttgatct
    28081 tcgttatatc ttgaccgtcg aataatatct caccatcagt aggttcattc aagcgaatga
    28141 tcgtacggcc agtagtggat tttccgcttc cagattctcc aactaaaccg aatgtttctc
    28201 cttcataaat gtgaaagctg atgtcattta cagcatgtac ttcattcttt tttcctacat
    28261 tgaaatactg tttcaatcct ttaacttcaa gtagtttatt tggcatcagc aattcctcct
    28321 tgaccgtaag tatgtttttg tttctcttta aagatagccc agcgacgttg gatttcttct
    28381 ggcggtgtca ctttgggtgc ctgcggagca agtagccaag ttgctgcctt gtgtgttttt
    28441 gatagttcaa aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt
    28501 ggatagaatg cgtctccttt tggcgggttc aataaatccg gtggagaacc aggaatagca
    28561 tacaatcgat cgttcgcact atctaatgtc ggcattgaac caagcagtcc ccaagtatag
    28621 ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa cttcaacgat tcttcctcca
    28681 tacatgacag cgacacgatc agcgacattc gctactacac caaggtcatg agtgataaag
    28741 ataattgatg tatcgatttt tgtctgaatt tctttcagca gctccaaaat ctgtgcttga
    28801 atagtcacat caagcgctgt tgtaggttca tcagcaatca ggatttccgg ataacatacc
    28861 agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg agaactgatg cggataactg
    28921 ttgatacgtt tctcagcttc gggtattcca actaatttaa gcaattctaa tgcagctttt
    28981 tgtccctctt tttttgaaac tttattatgc tttctaaggg actcagcgac ttgcttccca
    29041 atgggcatcg tcggatctaa agaggtcatt ggatcttgga aaatcatcgc tatttcttta
    29101 ccacggattt tttgcatctc tctttctgat tttccaacga tatcgttacc tttaaagagg
    29161 atttgaccag catctatatt ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc
    29221 actgattttc cacttccaga ttccccaacg atcgccaatg tttctccttt attcaaatga
    29281 aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga gatttctaag
    29341 tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt cattttagga tcaaaggcat
    29401 cacgcaatcc atcagcaagt aagttaaagc caatcatgat aaccgataaa gttactgccg
    29461 gaatccataa tagataaggc agatacagga atgtcttgta cccttcgctc aacatcgttc
    29521 ctaatgaagc tgacggaggc gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga
    29581 aaatagcaga tggaatacta aacatcattt gaataataat gacactagag atattcggca
    29641 aaatatgttt gaatgcaatt ttcattcttg attcacctaa tgtcaatcca gctaaaacat
    29701 attcttgatc ttttaatttc attgtttgtg cacgaacgat acgtgccatt ggtatccagt
    29761 ttgtgatggc catagcagcc acgattgaaa aaattcctgg ttcaaaaacg accaacatca
    29821 aaatcatgac aacgaggttt ggaattcctg ataagatttc caaaaaacgt tgcatcacgt
    29881 tatcgacacg tccgcctaac aaaccagaaa ccaacccgta agctacacca atcgttacat
    29941 ctagtaatgc agcaataaat gcgatcagca acgagattct cattcccata aataagcggc
    30001 ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt
    30061 ttgcttgagc atatttatca acaagttcac caccaaccat tgcttttcca ttcaatccat
    30121 cgatattgat tcccggaata cgaggaggca aattgatata agccacgttt tgggcagttg
    30181 gatcatgagg agaaacaaag attgtgatca cagagatcaa aatgatgacc aatagtaaaa
    30241 ccattgaaat aacggcagct ttgtttttct ttaaacggcg ccatgaatct tggataaaat
    30301 tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt atttttttgt aatggaagaa
    30361 attcatccgc agggatatct gcaactgttt cataacgctt tggtatcatt tccatttatc
    30421 ctcggctccc ttctgaaaca cgaattcttg gatcaactag accgtacaat agatccacga
    30481 tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt tacagccatg atcgtaggat
    30541 aatcatttgt tgtgattgac ttaacgaatt gttccccgat accaggaata gcaaagatat
    30601 tttctaccac cagtgatccg gtcatcaatg ctacagctaa cggtcctaat aaagtcatca
    30661 atggaatcaa actatttcga agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt
    30721 ttgcttttgc taattccaca taatcactat gcagtacttc caccatttcc gtacggataa
    30781 accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca
    30841 caaagccatc ccattttgcg attggcaaaa cttgtaattt gattgcaaag atatattgta
    30901 ataaaacagc aaatacaaag tttggaatgg aacgacctaa tatcgctacg agtgtagaag
    30961 atgtatctgc ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc gtccctagga
    31021 ctgttccaaa gataattgcc tgtaacccta actgcaagga tggtccgatt cttccagcta
    31081 acaaatgtgc gactggctgg tttttaaatt ggaaggaaat tccaaaatct ccttgaagca
    31141 aattagaaag ataaatccca tattgtacga taactggttt atccaaaccg acttgtttat
    31201 ttagcatctc gattgtttca ggactcaaac gttcctgatt cgtatatggc gtgcccggca
    31261 gcaattgcat caagaaaaag gtgattgttg caattaacca aagcgtgatg atcatgaaaa
    31321 agacacgttt taatacatat ttgatataac tattcactaa tgtttccccc tagcttttga
    31381 cttctgacag atgtttcgct acattattag ataatgaacc aaaaataaag gagcaatcaa
    31441 atgaaaaaaa aatctatccc ctacgcagta gcttttctac tgattctggt tattttaata
    31501 aaaaatctca tcaaccattc ctttacactc attcaattat ctaatgatct attcttatgg
    31561 tcattgccgt ttctgattat cggcggtttt ttatgggtct tttcttcggg tttcttcgat
    31621 cattttcagc gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa accagaattt
    31681 tcctcgctct cgtctgccag ctacggtatg tacagctttt ggctgatcat tgccggaata
    31741 ctaattgctc tttctgctat tttcatgctt ttttctttgt taggataagt gaatatgaga
    31801 acgaggttgc gacatttgcc gcagcctcgt tttttctcat gctcacggta ttaacgatta
    31861 ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct gccgcatggt gaacgatacc
    31921 tttgattttt tcattcacta ggtgaccttc tgctttttgg tacaccggaa cgacacccat
    31981 atcatcagag atgatcttga ttgctttttg gaaatcatcc caacgggctt gttcatcgcc
    32041 agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat tctgcattgc tccagcgacc
    32101 acggttgtat gaattacctg tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc
    32161 agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac
    32221 agagaatgga acaggtgttg gtttgactgt tacgccatcc aagttttctt ggattgagtt
    32281 ttgaacatat tcgattactt ttttcgatga atcatcatca gaagccatca aatcaaattc
    32341 taaagaatca acgcccaatt cttttttcgc tttttcccaa tattcttttg ctttatcttt
    32401 gtcataagaa actaattttc cagcttcttt tgcaaagtct tcgttcgttg ttggactttt
    32461 taccatatct gatgggatca atcctgtaga gacaactgat ccgtcaccta gtacttgttt
    32521 tactaaagct tcacgatcga tagaataaga aatagctttt cttaggttca cattgttgaa
    32581 tggtgaattc ttttcacgtt gattgaattc gatatagctt gtccgcgctt ctttaacaga
    32641 agtataagct ggatcgtttg cgttttgctg tgctaactca ccagacaaga tcacgtcatc
    32701 tgcttggcca tctttgaata agttcaatgc agtagaagat tcttttacta cactgacttt
    32761 gatttcagaa agttttactg tatctttgtc ccagtaatga tcatttttct tataagtcca
    32821 ttctgtatct gttcctggtc catcaaaatc ttctaatgtg aatggtccat tataaacagc
    32881 tttatcactt gcagatgcat aggcatcccc attttcttcc actacggatt gtttttgtgg
    32941 gaaaaatgat gggaaagcaa gcagatattg gaaatatggt gtttgtttcg tcaatttgat
    33001 ttctaattca tagtcaccaa ctgctttgat ccctagttct gatttatctt tctttccagc
    33061 tgtaatgtct tctgcatttt caactggcgc atacaggtaa gcatattcag aagctgtagc
    33121 tgggtctact gttcgttgcc agccataaac gtaatccgct gctgtaacag gatcaccatt
    33181 tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt cactgacttc
    33241 tgccaactcg ctagctcctg ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc
    33301 ttcataaacg ttattcaatg ctgtaaaact gattgtatct gttgcaactg ataagtcagc
    33361 tgtaggcatt tcttgaggca cgactaagtt aaatacgcct cctccatctg ctgaaccgct
    33421 ttttgctgaa ctactgcttg tggctgaact gccgccataa catccagcaa gtacaaggcc
    33481 gcataccgca accaaaccaa atgtaaattt ctttttcatt ttacgtaccc ctttattgga
    33541 attttcagaa taattaatat tttctgaagt tgtttaagag tttaatgaat aattattaaa
    33601 aaatcaacac ttttttttgt ctttttcata aaataatgat tttttcgtga ggtttcaagg
    33661 aatttttcag aaaaaatagc tttgtacaag gttttccatg gtcttccaaa agccattaaa
    33721 taattttcgt tatattgtct attattgcca tacaaagata aaaaagaata aacaaaaaga
    33781 aactgtgaca aaagtcttgt cacagtccct tatctgaata aacggtgttc aaaagccagt
    33841 ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ccattttcac aaaatttttc
    33901 ttactactcg aaactaagcg actttttcac aacctctggt acacggtgtt ccaaaagtag
    33961 cttcttcgga aataagttgg aatttcacaa aaattcaaga agtaattttc gtaaattcct
    34021 tcttatttct tgaagctgat tacttctgtc acaacctctt tttattattg agaatttggt
    34081 cgaggaataa tcgtttttgc catccaaata tccggtttgt cccagccatt tgcatttggt
    34141 aagacaccta cgattttata acctaatttt tcatagaatt catacggatg ttcacgaagg
    34201 ttctggatgg aagccacttt atcaaatgta tgctcataca ggtccgtttg acttaacgtt
    34261 gttccatggt ctaaatcatc cgtacctaaa taaatcgtga ttcctcctct ggaagctact
    34321 tctttttcta agtaattgac taatcgagta cctatttggt tctttcgtcg ggagctttct
    34381 acaactaatg gatgcaattc ccaacctgtg ataccgtatt gagggattgc accaataaat
    34441 cctactaact catcttggtc taccgctgct accgcgattc gttctggatt catcatttct
    34501 tctacttctt ctgccgagct gtctccatat tcttccggcc aagtcagtct cagtaaatca
    34561 gaaagctgat ctttcaatac tggattatta cggtcaaatt cactgattat catacgtctt
    34621 cccccttgtt cttgcttatt ctaacagtcc ttttgaaaaa acgaaatgtt ctcgctcaaa
    34681 aatctttagc taccaatcag tatctctatt ctttcattat ttatcttact tataaaataa
    34741 aaaaattata cagaaacact ttattcaaaa taggaaagat caggaaagcc atcaattgga
    34801 ggaaaaaact ctgtttgttc atcatgatat agattatctt ttgctttaaa aaaaaccaat
    34861 tatgaattga caaataaaac ctgttttcat ataataatct taatttttag aatttttaga
    34921 taattacaaa aaagcaaagg gaatcggtag aaatactaag aggaacaaaa attcattatt
    34981 gtcgttttca atgtttttgt acttgttcgt ttttaggata cttattgatg atttcaaggt
    35041 gttcatgcca ccttattcat cacttatcct gtcttaggtt cacctctact ccatctacag
    35101 ccgcagtcag atcgatcact ctttaaaatt ttaacccgtt tcaacgaaga aagaaggaaa
    35161 tgaatatgag tcaaagtgtt ttcttaccaa attataccat tggaacagat gcgtatgaaa
    35221 aagcaaatgg aatcgtcaaa gaatatggga caaaagtagt gctgattgga gggaagactg
    35281 cactttcaaa ggcagaaaac aaaataaaag tagctttgac acaggaaata gaggttgtcg
    35341 ccagtctctg gtacgggggt aattcaactt atggaaatat tgaacagttg atccagcatg
    35401 aaagtgtcca aaaagcagac gtcctgtttg cagtcggtgg tggccgtgct tgcgatactg
    35461 taaaagtcat tggtgaaaaa ctagctaaac cagtcttcac ctttcccact attggttcga
    35521 actgttcgcc cgtcactgcg gtatgtgtca tctataacga agaaggcgta atggacggac
    35581 tcttctttcc ttcaaaacca cctgtacaca ccttcattga tacagcaatt attgccgaag
    35641 cacctgataa atacttatgg gcaggtattg gcgatgcttt atccaaagaa gtcgaatcta
    35701 ctttttctgc tcgtggcgat gatctaggct acacggatag attaggaatc caaatcggaa
    35761 aaatgtgcat tgatcgtctc ttgacttatg gtacgcaagc attgaaagat agtgccgacg
    35821 atcgtccctc atatgcaatt gaacaagtcg tttcggatat cattgttact accggattgg
    35881 tttctgtttt agttaacaat aactataatt ctgctatggc acactcattc tattacggta
    35941 cgactgttct agaaaaaatg gagggatacc ttcacggagc atctgtcagt tatggtgtac
    36001 tgaccttatt gatgatggat cagcagttcg atttgtttga taaaatctat gtgtttatga
    36061 aaaagaacca acttccagta tcgttaaccg acatgaatct caatgtcacg accgatttag
    36121 aagcaatctt agataaagcc atggcaacaa atgatattaa acatgtgcct tatgaaatca
    36181 cccgagaaaa atttaaacag gccattcttg acgtagaaac ttatcatgct acacaagaaa
    36241 gccagcaagg atataagaaa atcagttaaa taattgctaa aacccccatc aagcaggctg
    36301 tcatcaaatt gaggacattt ctttgcttga tgagggtttt tcttgtcaaa aaaagcttta
    36361 tagctttcgc tatagaatct tttctcttat ttaagttgaa cagttcctta attaagaaaa
    36421 ctgctccttt tatggtaatt gaaaaaacga gtaggatacg attatttggc aactgttttt
    36481 ttcatttgct tgcttcgcaa ttgtccgcaa gctgcatcaa tatctgtacc atgttcttta
    36541 cgtatcacac agtttacgcc gtttttcttc aatacatcat aaaatctcaa tacatcagct
    36601 tttggactgc gagaatattg atcatgttca ctgactggat tatatggaat caagttgaca
    36661 tatgctaatt ttttcttatc ttttaataga tctgctaatt gttgcgcatg ctctggtcta
    36721 tcatttactt gattcaacat gatatattca aacgtgattc gacggtttgt tttctctaaa
    36781 tactcatcca cagcttccat caatttctca attgggaaac ttcggttgat ccgcatgatc
    36841 gatgttcgca cctcattatt cggcgcgtgc aaggaaatag ccagattgac ttgcaagccg
    36901 ttattagcga attccttgat tttgtgtgct aatccactag tggaaactgt aatgtgccga
    36961 gcaccaattg ccaatccttt ggcatcattg atgatatgca aaaagtccat tacattgtcg
    37021 taattatcaa atggctcacc gatccccatt acaacgacat gtgaaacgcg ttcgccaaga
    37081 ttgcgctcat caaaataatg ttggaccatc atgatctgtg caacaatttc tccagcagtc
    37141 agatcacggt tctttttgag cagcccgctt gcacaaaacg tacagccgat attacatcct
    37201 acttgtgtcg tcacacaaac tgacaagcca tattcttgtc tcatcagtac agtctcaatc
    37261 atgtttttat cagggagttc aaacaaatat tttactgtac catccgaagc ttcttggacg
    37321 accatctgtt ttagaggatt gatgataaaa tgctccgata atttttcgat caattgtttc
    37381 gatagattcg tcatttctgt gaaattactt acacgttttt gatacaacca ttcccaaact
    37441 tgggcagctc ggaatttttt ctcgccgttt tctataaacc aattgattaa ttcttcattc
    37501 gtcaagccat aaatggactg tttttccaaa gtattttccc tctttttctc aaagtttcgc
    37561 tacatactat acatgattca tttccttaat gcaatacttc acaaataaat cgaatagaac
    37621 tttaatctca caaaaactaa aaagacaaga catcataccg tattagaaaa gatggaaaaa
    37681 aaccattttt tcttttggta tcatcatgcc ttgtcttttt attggttatt cgtttttcaa
    37741 cccttcatca cacaagtttt acatgtcatt atcgtccggc aacgtttctg atgtttcttc
    37801 ctgctcaatg aatactttcc gaggcttgct tccttctgac gggccaacaa ctccatgagc
    37861 ttccagttca tctactaaac gtgctgctcg gttgtaaccg atccgaaatc ttctttggag
    37921 aagggaaata cttgccgttt gcatttcaac gacaagtgct tttgcttctt caaacagctc
    37981 atcttgtggc tgttctggag cacctgtcgt ttcaacttca tctgtcggca tcattttctc
    38041 ttcataatga gcttcttgct gatctgtaac aaattgaacg acccgttcta cttcgtggtc
    38101 agaaatgaat gctccttgca cacggatcgg tttattctca cccattggca agaatagcat
    38161 atctcctcgc cctaagagct tttctgcccc attagaatca atgatcgtcc gagaatctgt
    38221 accgcttgat accgcaaatg ccattctgga aggtacattt gctttgatga ttcctgtgat
    38281 aacatccaca cttggacgtt gagtagctag gatcatatga atccccgctg cccgagccat
    38341 ttgcgctaaa cgaataatcg catcttcaac ttcattgctg gctaccatca tcaaatcagc
    38401 taattcatct acgatcacta cgatgaatgg taaggttgga tgtttcgttc cgttttccaa
    38461 gtttttctgt tgaacaaatt cattgtaacc agaaatattt cttactccgg ttgccgcgaa
    38521 tttttcatat cgttcttcca tttcttgtac tactttttgt aaggcttgcg ctgcttttct
    38581 agggttcgtc acgaccggcg tcaacagatg aggtatgccg ttgtacatat tcaattcaac
    38641 cattttcgga tcgatcatca tcagttttac ttcatggggc ttagcacgca ttaatatact
    38701 tgtgataatc ccattaattg ctactgactt cccactacca gtcgaaccag ccacgagtaa
    38761 gtgaggcatc ttcgaaagat cagccgtttg tacccgtcca gaaacatcac gtcctaaagg
    38821 tacttctagt aatttatctg ggtggtttgg ttgtgcttcg atgatttctc taaaggaaac
    38881 catactgatc tttccatttg gtacttcaat accaatcagt gatttacctg gaatcggtgc
    38941 ttccatccgg acatctttcg ccgctaaagc aagcgcgata tcatctgtta agttcacaat
    39001 tttacttact tttaccccta cagccggctg gacttcgaat tttgtgacgg atggtcctaa
    39061 gctggctttg acgaccttgg catcgacacc gaaactttgg aaagtttgtt ctaatacgcc
    39121 gatatttttt tcaatttttt tatactcatc actttgatcc acagttggta tagaatcaag
    39181 caaatcaact gtcggtaatt catagtcttg attttcctgt tctgcttcaa tctcaaattc
    39241 taatgaggta ccgtcatcct ctggcaactc atctgaaaaa ccttctgctg tattggtctg
    39301 ttttggtgta tctggtactg gtttctctag attcgcagga tggatatttt cttgaaaact
    39361 gtcgattgga acaaaactta gctgctccgg ctctgtattt tctggttcct gccattcatc
    39421 agcagtattt tccctttgct ggctcaccgc ttttttcttc tcatattctg cagcttcttt
    39481 ttctgcagct tcttttgcag ctaagcgcct tgcttctttc gcttctgcgc gctgtttcat
    39541 tctctcttcc tttgccgctt gctttcgagc ttgcttggca ggatcacctt ccaataattc
    39601 ctgtactcgt tctccaattg attggatacc atttaacagc tgatagcctt ccagattact
    39661 gaacaggaat gctcctcctg ccagtaatag cactgctgct aaataacttc caggctgtgc
    39721 aatcaagaag taagtaaaat gataaaggat tgctccaatc atcccaccac caacgttatt
    39781 gtctacttga ctttgtttta tatcactggc taacaaatcc catgtcgtcc ccatgatatt
    39841 tggttcccca gtatggagtt tgccaaataa atgggcatgt aacaataata agataccgaa
    39901 ataaaagagt atgccgccaa accagcgtcg gtttttacca attgaaaact ctgtattctt
    39961 aatgacaatc caaaatccta atacggctaa taaaagacag aggatttggt aagtatttcc
    40021 tgcaatgatc cgaaagccgt ttgcaatcaa tattcctaaa aaacctaatt ttaaaaagcc
    40081 gaagacagca aataaaatca aggcaaaccc aacaccaatc gtcagcattt gttcctgctg
    40141 ctgtttttgc tttttcgttt ttttcgtctt tttccttttt ttagtaggcc gtttcttttg
    40201 tgccatttat tatccctcat ttcatcaagt ttcattatac aaaaaaaagt aagggaacta
    40261 aagttttgtt tgaaagaact gacgctttct ttcatttttc agcttatttt acagtttttc
    40321 ttaatttcta aatgatttta tcaagaaaag gcacggccat atccagccgt accttcttgt
    40381 tttaagtatg tgaactaaag gttctctcca ttttcttcaa tcagtttttg gtaccagtaa
    40441 taactgtctt tcttatatcg ttccaaagat ccttcttgtg tttcgtcttg atcaacatat
    40501 acaaatccat atcgcttttg ataaccgttc agccagctaa gcaaatctgt ataagaccag
    40561 gtgcaatagc ctaggacttc tgctccatcc gaaattgctt ctttgatggc tttgacgtgg
    40621 ctttgtaagt aattgattcg atagtcgtca tggatttgtt tatcttctgt caacttgtca
    40681 tactctccta agccattttc cgtaatcagt accggcatgc ggtaacggct tgtgatacga
    40741 cgcagtccga tccttaatcc ttctgggtca atctcccagt cccaatttgt tcgttcgaca
    40801 aatggattct caactttttt atacattcca ggaactccag tttcttctga tgaacctttt
    40861 tttcctgtcg tattcatttt tccagcacca actccgttta atggattgaa tgcattcgtc
    40921 gccgtttgat agtaattgat cccaagaaaa tctggtttag cagattctag caattctgca
    40981 tcgccagctt caatcgttgg tgcccatcct ttttcttcta agaacctcat ggcagcaatc
    41041 ggatattttc cccacatata gacatccatc caataattgg ccatcaggtc ttctgcattt
    41101 tcagcagcca gaacattttc aggattactg tcaattggat aatttggcgt atacgcaaag
    41161 cttggtccga tttttccagg catttctaac tcgtgaaatt tattgatgac agatgcattt
    41221 gctaaattag caatatggtt aacttcatac attcgtttcg ggtctgtcac acctggcgga
    41281 tgtgctgcta acaaataacc taatgaagtg aagatatttt gttcattcaa actaatccag
    41341 taatggactt tcccgcggaa tgcctcaaat aatgtttctg cataatgagt gaaatcttca
    41401 atgatctgac gtgattccca accattatat tcatcttgca atgcttgtgg aagatcccaa
    41461 tgatataaag tcaacatagg ttcaatatcg ttttcaatca attcgtcgat caatctctca
    41521 taaaaagcga gcccagcttg attgacttct cctcgtccat taggaaaaat ccgtgtccaa
    41581 gctacagaga agcggtaagc tttcagacct tgttctttca ttaaagcaat atcttcttta
    41641 taacgatgat agtggtctac tgccaaatca ccgttcgttc ctttaaatgt ctttccaggt
    41701 atccgtacaa atcgatccca gactgattct cctttcccat cttcttgcca agctccttct
    41761 acttgatacg ctgcagaagc agagccccac agaaaatctt ttggaaatga ggtaagttct
    41821 ttgtgttcca ttcatctctt cctttcctat aaccttcaat cgttcctctt ttagaataac
    41881 acgaaacccc ttccacaaca aaataaaatt atgtaaagga ggttgtgaaa aagtcgctta
    41941 gtttcgagta gtaagaaaaa ttttgtgaaa atggctttct acatttttgc ataaatttgg
    42001 cttaataccg aaaaactggc ttttgaacac cgtttaccaa gaggttgtga gcctgccatt
    42061 cagctccgag tcacaaggag ttggcaggag aacactgttt attcggaaaa agggactgtg
    42121 acacgtcttt tgtcacagtc ccctatttgt taatttctcg gcgtaacatc ttcgccaaac
    42181 catttatcgg atattttttg gaattcacct gttttctcta gttgatctag ggcttcattg
    42241 atttttttca ctaatgtact atctgacttg cgtgcaccaa ctgcaaaatc ctcactctca
    42301 taagaacctt ttgttatgtt gaagtcttct agttcgtttc tttgtttcaa ataatagtct
    42361 gcgtatactt tatccatcag cagaccatcg acccgtctgc ttttcagatc gatcaatgct
    42421 tcattaaaac tatcatacaa aacagcatct tggtttttga ctatctcttt taatacttct
    42481 ggttgtttcg taaatacatc ataaccagat gacccatttt gcgcacctaa tctcttatta
    42541 ttcatttgtc caaagtttgt gattcctgat tctttaggtg tgatcagcac ttgatcgttt
    42601 tccatataag gttttgtaaa ttgcactttc ttttctcttg ccggcgtttt ggaatagccg
    42661 ttccatatca aatcgatcgt tccattgttc agttcaaatt ccttcattga ccagtcaatg
    42721 ggctgaaagt ctaccgtaat cccatattgt tcaaagactg ctttggctaa atcaatatca
    42781 aatcctgtga gcttcccgtc tttatctcta aatcccatag gaacaaatgt atcgtctaaa
    42841 cctataacga tttttttctc ttcgttgata gtcgcccaat tatctgctga agtttttttg
    42901 ttcgaacagc ctgcaaaaag aagaagacct gcgatcaaag cgaatagaaa actggttttt
    42961 ttcatctcta tcttctcctt tcttaatgaa ttggggaaac ttttagtaat tgatcaccga
    43021 cattttctgc aaatactaaa tcatgggtga caataatttg ggttacccca tcttctttca
    43081 atgataggat caaacgctcg acttgctggc gtaattcagg atctaaagca cttgttggtt
    43141 catcataagc caatacccgt ggattcatag ccaatgctct ggcaatcgct acccgctgtt
    43201 tttgtccgcc agaaagctga taaggataga tattcaataa atcactaatc cctaatgaag
    43261 ccgctaattc ttctgctttt ttggtgtatt cttcttttgt ctgtttcaat accatttttg
    43321 gtgctaaggt aatgttttca aaaatattca gatgtggaaa tagctggaag tcttggaata
    43381 cgactcctac aacttgttct ttcattttaa gctcgaatgg gttgaacggt acaccgtcta
    43441 aataaaattc tccgctgtca gaactttcta agccagccaa gatacgtaaa agcgtggttt
    43501 taccgccacc ggaaggacct acgatagcta atgttttccc atctggaatg attagattca
    43561 aatctttgat tatttttttt tcaccaaatg ctttgttcac tttcttcaat tcaatcatat
    43621 ttcttcctcc tatcagttag cggtaataat ttaattttcc ttcaacttgt ttcattatga
    43681 ttgttagaat accagtcaat gtaagataga tgattgccac gcctagcaaa ggcagtaaag
    43741 tcgcgtcgcg ttccatcgcg attttcccag cacgcattac gtcacctaaa cctaatatat
    43801 agatcaagga agaatctttg accaagttga taacttcatt ccctacagaa ggtaaaacaa
    43861 tctttgtcac ttgtgggatg acgattttcg tcaccgtttg aaagggactt aattgcaaaa
    43921 cttgcgctgc ttcatattga ccttgaggaa tcgattgtat ccctccacga aagatctctg
    43981 caaaataagc tgcatagttt aagataaagg caatgagtgc tgcttcgaaa cgatcaaaaa
    44041 cgatcccaat cgtcggcaac ccataaaaaa taaaaatcag ttgcaacaac agcggtgttc
    44101 cccgcataac ccatacatat gtttgaagca agtagcgtag cggcttaaaa tgcaaagaca
    44161 atcctaatgc taaaacaatc cctaatggta atgagccaat caaggtaaat aaaaataatt
    44221 tcaatgttgt gagtgctcct actaataatt gcggcataac ttcaatcaaa tactccatct
    44281 tcttttcctc ctttaaaata aaaaaatcct cttggcaaat gccaagagga cgtattatac
    44341 gtggttccac ctctgtttat tttttccttg cagaaaaaac ctcacacagt cgttgccgtt
    44401 cagatgaaaa caaaaagtcc tttgatccga agatcaaagg acgttaatca cgtggttcca
    44461 ccttttttta ccgactcctc acggaaaagc cagccttatc tggtcatcta gcaacgaccg
    44521 cagctgtaac gggctccccg gattttccta ctatacgctt cagaaaatcg actccaagat
    44581 gtgtttcacg gttaactgct gcctctttcc accaaccaga gactctctaa aaacacctaa
    44641 cggctactct tcttttcgat gtcatttgtt ttaactttac gtgtaattta catgattatt
    44701 tttcatttgt caactctttt ttatgataca ctaataaaaa aaggagggtg atgcgcgatg
    44761 agtcgtgcaa ccgaacaaga agcttatgaa ctactaaaaa aactgggtat ctcttttcaa
    44821 aaagtcgatc atcctgcgat tacgtcagtc aaaaatgtcc ctttttccct acctggtccg
    44881 caagtaaaaa atctattatt gaaatcaaga aaaggaaaac aaatctatct tgtcatcttg
    44941 cctgatgaaa agcacgccga tttaaaacaa ctagctgaac aattaaaaga aaagcgctta
    45001 tcttttgttt ctgaagaaca gcttcctgac ctcctaggcg ttccagcagg tacagtgact
    45061 ccattagcat tgatgcacga caaagagaaa aaaatccaag tcgtgatcga tgctgctatt
    45121 gatcgtcaag ataccgtagg ctttcatcca aatatcaata ccactacgtt gattatggat
    45181 ttttccgact ttgaaaagat acttgtttac ttagaacatc tgccagttta tgaaaatttg
    45241 taatgataga gtagttttct tatttcccaa ttcaagcgtc tttttcttta tttagcacaa
    45301 aatatatcag aaattctttt ggccaatcaa acaatgtaac aactagtatg aacgctgaaa
    45361 actaatacga gtattcttcc atagttagcc aaatccatat ttattgattc tacgaaaaaa
    45421 gaagcatata taaaaatgct tcttttttcg attataaatc ggaattttat tcttttgatt
    45481 tcagtcata
//
LOCUS       DACCWK010000015        44209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCWK010000015 DACCWK010000000
VERSION     DACCWK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.015"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            54..731
                     /locus_tag="IVW65_000728"
     CDS             54..731
                     /locus_tag="IVW65_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899651.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            734..1861
                     /locus_tag="IVW65_000729"
     CDS             734..1861
                     /locus_tag="IVW65_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899652.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2061..2501)
                     /locus_tag="IVW65_000730"
     CDS             complement(2061..2501)
                     /locus_tag="IVW65_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7899653.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(2512..4545)
                     /locus_tag="IVW65_000731"
     CDS             complement(2512..4545)
                     /locus_tag="IVW65_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7899654.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4547..6250)
                     /locus_tag="IVW65_000732"
     CDS             complement(4547..6250)
                     /locus_tag="IVW65_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7899655.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6344..7294)
                     /gene="manA"
                     /locus_tag="IVW65_000733"
     CDS             complement(6344..7294)
                     /gene="manA"
                     /locus_tag="IVW65_000733"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7899656.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(7333..8358)
                     /locus_tag="IVW65_000734"
     CDS             complement(7333..8358)
                     /locus_tag="IVW65_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7899657.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(8567..9436)
                     /locus_tag="IVW65_000735"
     CDS             complement(8567..9436)
                     /locus_tag="IVW65_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7899658.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(9895..11787)
                     /locus_tag="IVW65_000736"
     CDS             complement(9895..11787)
                     /locus_tag="IVW65_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899659.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11784..13511)
                     /locus_tag="IVW65_000737"
     CDS             complement(11784..13511)
                     /locus_tag="IVW65_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899660.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13527..14177)
                     /locus_tag="IVW65_000738"
     CDS             complement(13527..14177)
                     /locus_tag="IVW65_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7899661.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14381..14677
                     /locus_tag="IVW65_000739"
     CDS             14381..14677
                     /locus_tag="IVW65_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7899662.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            14849..15469
                     /locus_tag="IVW65_000740"
     CDS             14849..15469
                     /locus_tag="IVW65_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7899663.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15561..16727)
                     /locus_tag="IVW65_000741"
     CDS             complement(15561..16727)
                     /locus_tag="IVW65_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7899664.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16739..17092)
                     /locus_tag="IVW65_000742"
     CDS             complement(16739..17092)
                     /locus_tag="IVW65_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7899665.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17220..18242
                     /locus_tag="IVW65_000743"
     CDS             17220..18242
                     /locus_tag="IVW65_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7899666.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(18681..19115)
                     /locus_tag="IVW65_000744"
     CDS             complement(18681..19115)
                     /locus_tag="IVW65_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899667.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19225..21084)
                     /locus_tag="IVW65_000745"
     CDS             complement(19225..21084)
                     /locus_tag="IVW65_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7899668.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21202..22362)
                     /locus_tag="IVW65_000746"
     CDS             complement(21202..22362)
                     /locus_tag="IVW65_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP7899669.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22669..24093)
                     /gene="araA"
                     /locus_tag="IVW65_000747"
     CDS             complement(22669..24093)
                     /gene="araA"
                     /locus_tag="IVW65_000747"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP7899670.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24109..24807)
                     /locus_tag="IVW65_000748"
     CDS             complement(24109..24807)
                     /locus_tag="IVW65_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7899671.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(24764..26425)
                     /locus_tag="IVW65_000749"
     CDS             complement(24764..26425)
                     /locus_tag="IVW65_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7899672.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26643..28043
                     /locus_tag="IVW65_000750"
     CDS             26643..28043
                     /locus_tag="IVW65_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7899673.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28295..29374)
                     /locus_tag="IVW65_000751"
     CDS             complement(28295..29374)
                     /locus_tag="IVW65_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7899674.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29460..30419)
                     /locus_tag="IVW65_000752"
     CDS             complement(29460..30419)
                     /locus_tag="IVW65_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7899675.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30431..31954)
                     /locus_tag="IVW65_000753"
     CDS             complement(30431..31954)
                     /locus_tag="IVW65_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7899676.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(31964..32527)
                     /locus_tag="IVW65_000754"
     CDS             complement(31964..32527)
                     /locus_tag="IVW65_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7899677.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32533..33396)
                     /locus_tag="IVW65_000755"
     CDS             complement(32533..33396)
                     /locus_tag="IVW65_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7899678.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33410..34306)
                     /locus_tag="IVW65_000756"
     CDS             complement(33410..34306)
                     /locus_tag="IVW65_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7899679.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34481..35752)
                     /locus_tag="IVW65_000757"
     CDS             complement(34481..35752)
                     /locus_tag="IVW65_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7899680.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35742..37259)
                     /locus_tag="IVW65_000758"
     CDS             complement(35742..37259)
                     /locus_tag="IVW65_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7899681.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            37457..38347
                     /locus_tag="IVW65_000759"
     CDS             37457..38347
                     /locus_tag="IVW65_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7899682.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(38420..39244)
                     /gene="nadE"
                     /locus_tag="IVW65_000760"
     CDS             complement(38420..39244)
                     /gene="nadE"
                     /locus_tag="IVW65_000760"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7899683.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(39256..40728)
                     /locus_tag="IVW65_000761"
     CDS             complement(39256..40728)
                     /locus_tag="IVW65_000761"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7899684.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(41365..41726)
                     /locus_tag="IVW65_000762"
                     /pseudo
     CDS             complement(41365..41726)
                     /locus_tag="IVW65_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
     gene            41949..42701
                     /locus_tag="IVW65_000763"
     CDS             41949..42701
                     /locus_tag="IVW65_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7899685.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(42841..43764)
                     /locus_tag="IVW65_000764"
     CDS             complement(42841..43764)
                     /locus_tag="IVW65_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7899686.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(43779..44051)
                     /locus_tag="IVW65_000765"
     CDS             complement(43779..44051)
                     /locus_tag="IVW65_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899687.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        12934 a         9167 c         7617 g        14491 t
ORIGIN      
        1 ttagatgttt tccattctat tatgttatta aattttatat tggaggccta tctatgacat
       61 tactgatcaa aaacatgaac atgaaacgtg gtaaaaaacg tgtgattcga caattgaatc
      121 ttttagtaga atcaggagaa gtagctgctt tgattgcccc gaacggattt ggcaaaacaa
      181 ctttgctgaa tggcattgcc caactgctgc caacaactta tgatcaattg tcccttcaca
      241 acatctctcc tactgcttat acagattatc gccggtcttt cttttttcta gaatcaacga
      301 atcagttatc cgactatcta tcggctgttg attatttgac tttgatcaaa tactattggc
      361 agagcaaagt agaatgccaa actgttttcc aacgattgaa gatgactgag taccttactt
      421 tgccgattgg aaaaatgtct ctaggaatga aacaacatgt cttgcttgct gcttacttga
      481 tcagtgattc atctgtcatg ttattcgatg aaccgttgaa cggattagac ccaggaagta
      541 ttgatatctt gaatagcctc atcagacaat ggcagaaaga ggggaagacg attcttcttt
      601 cttcccacgc tatcgcgaat atccaatcga tttgttctaa agcatgtttt ctaaaaaatg
      661 gagaaatcgt cacagaatcc tcaaatatgg aagagatttt agctatttat cgtaaattat
      721 atgaggtgta actatgtctt tacttacatt tgaacgcaat ctcttactaa aaaacaaagt
      781 aaacttttta tttcctctat tgttggtcgt gctctttgca ttcccacttt tttttgatca
      841 taaacttgct tatacagaat tcgatgaact gaatcataac tatgaagaga tgcaacgttt
      901 gattgaaacg ttgaaagaag atgagaatga gaaagaattt gtggaaagtt tagagaaaag
      961 caacaaactg atcgaagcaa tcttacatgc aaaaaacaca gggaatgtcc agcaaatggt
     1021 cgaagcaact tatcattatg aaaaagatat actggatcga ctgatttctg gtcaaagaca
     1081 aggaattcca atcattgaac aacaaaaacg tgtcgagttg ctccgttata tgaaagaaca
     1141 tcagatccaa agatattcta tttttgactt gccagcgcat ctccctttag caaattacta
     1201 cgaaaatatt ttttcaggaa tgatttctag ttttttgatt ttatgtataa ccgccttgtt
     1261 tctttcttcc atcatctctt atgaaaaaag aaagcaggta atctctctag tcaatctatt
     1321 accggatagt atggtaaaga aacatagcat ccgttttacc atttattatg gtgcagctat
     1381 gctaagtctc gtgatgccat ttttgatcgt ctctatacta gtaataatca aaaatggttt
     1441 gggagacttc cgttatcctg taggaacgat cattggtcag gagatacgaa tccttccgat
     1501 gtatgagtat cttttccaga gctttctgtt cctgttgttg tgggtgctct ttttaagtac
     1561 aatcagtttt cttctatctg ctttatttga acattctcta gtcaatcttt taggtacact
     1621 tctttgtctt ttcttagcag agtatcgctt gttttcttcg attggatgga tagaatcaat
     1681 cagtcattac ttgccaactt cttatgttga ttttcaaaat gtgatcatag gaggagatat
     1741 tttctcgcca ctagcttcag aacaggttac tttcatgaat ggtatcttga cacttggtat
     1801 atggagtatc gttttattat tcataggtat gggcactatc tatataaaaa agagctatta
     1861 aaacacaaaa ataatagggg cggtgacaaa aaccttgtca ccgtccccta tttcaaataa
     1921 acggttttct agaataaata gttttcaaaa gcttgcatcc gcagtaataa gaacctccca
     1981 acaaaaatag ctcctcttat ttttatcttg gttgaagtta ttacttgatg caaggcagct
     2041 ttttttactg tgaacattgg ttatatatgt ccatgttcaa aaatcatttg gacttcttca
     2101 atggttttcg catcactgag cgccattact tggtcgatgt ctgaacagaa aaaggctaaa
     2161 tcttggagca ccgctaactg atgttctttt tttaaagcca ctacaaataa gagtgtcgcg
     2221 atttttggtt cctcttttgt tccaaagtcc acaccatcag gcacttggac taaatagatt
     2281 ccttcttcta ctacttcttc atctttgtca gtcccatgtg gcaaggctac gaaattcccg
     2341 atatagacac tcgctttttc ttgtctttcg atcattgatt ggatatatgc cggtttgatt
     2401 cccccttgtt gggtcatcca ctggccgatc tcggtcaacg cttctttcca attttgatag
     2461 gattgatcga gcaatatttt ttctgctggt agctgtatca tcttttcacc ttcatttctt
     2521 tatttcttca atcaggattt tcgctaacaa ctgataaacg atttctttat ttcctgagcg
     2581 gaagatctcg gtgttcaagt cattcatgat gattgctcca ctgatctttc ctaaaagaat
     2641 cccgtctttt tcttggattg gcgaaggcgc cagcataact aacatacggg acaaatcgac
     2701 tggctgtcta tccatccctt caatggtcaa cggatgtttc aaattgaaaa tacaaaagat
     2761 cggtttagaa atcaatgagc tggatgtatg gaataacccc atatttgtat gaggaatacc
     2821 gataggtgct tgttcatatc gcttaagcag ccggcgatga accacctctt catccgttac
     2881 tagtccttca ggaaaataag gaaataagct gcgaatcgtt tcggaaatcg tctcaggatt
     2941 atccagttct tggataaaaa aacgaccgag taattgattg atttgatcca tgaaatgcat
     3001 tgtttcctca taagtatctt ctatcatcca ctcgttattg ctgttatacg taattttttg
     3061 cctttttttc ttctcaatat gcgaaaaagc ttcttttagc tgcttgactt catcttctag
     3121 cagtagcggg gagactaaaa tatattttcc accgtatcct ggtaaaatag atgtagaaat
     3181 aacaacatca tattcttctt gtaaatcgat tttttttaaa tcagcaatcc gatagaaatg
     3241 aatttgttgg atgaagggaa aatacctttt caatcgtagt tccaacatgc tagtagaagc
     3301 taatccactt ggcgaaaatc ctgcaatact gatattgctt tctttcggac ttcgttccaa
     3361 tgaattcgca aaatgaagga ccatataagc gatctcttct tcggataatt ttttttcttg
     3421 aaaaatccgc tctactgctt tacggattgc attcgcaact tcctcgtatt gaaccatgat
     3481 tttttctaaa atcggattgg tcagtgtctc ttcttgtaaa acggctcttg aaaacacacc
     3541 agataaatga gtcaacagca ttttgtacaa agtttcatct tcataaaaat ggacattcgt
     3601 ctcttcactt acttcttgaa tcagttgttt tactaaataa gcaagctgtg tatcgaacgt
     3661 ctcttcaaag aaattctgat caaatgagtt cgtgaagtca tttaataatc ctgcaaaaaa
     3721 gacgatctca tttactggat aaagttgttt tgtctcagaa gcgatccgtg aaaacaatcg
     3781 tttagctgtc tgtagtaatt ctttgtttgc cagactatta tatgtctcat ttgtcagtaa
     3841 atgattttta gtcacacgct ccatcgtcaa tgtcagcatc aaaaccaaat gttcaagctt
     3901 tctatcactt agattggtct ttttttggat caattcttcc ccgattactt tgcgtgcaag
     3961 agatagatgt tgcggatcaa cgaatcgaaa aaaataattc agtgatgcat cgaaagaaat
     4021 actatgaaaa agttcatatt cattgatttc taattctaat acattcgctg tcagaagccg
     4081 gcggtatttt tccggtcctt cgatctcgta tccctggttc cgaatgattt ccaaaggcag
     4141 ttggcgaata ctttcttcca actgtttgat atctgcataa aaagtggtat tactgatgcg
     4201 atatgattcc atgaaatgag caagactcaa cggctctgtc gtcagtaaca actcggctaa
     4261 aatcgcgtgt tgtcgtttag cagtggataa gtcattactt ttcccatctg tattttcttt
     4321 taattgctct aatacttctt ctgatgcctc aatgacaaat gagcctctag ctgctttttt
     4381 caaagaagca tttaccgatt tcaatgagac ttcaagatta tctaattcgc ggtaaatagt
     4441 ccgtttactg accttcaata aattcatcat tttttggata gatacaggtg acgggctact
     4501 gagaaattct gctaataatt gtttttctct gggcgataaa tacatcttat atttctccct
     4561 tcacgagttt ttcgtattca ttcgtagcta cccaatgttc aactagaact agccgaggga
     4621 tcttttcttc tagctgttca gttacagcta gttctctttg tgcgatataa atcgtttctt
     4681 cttggatttt gattttttct attgattctt ttgagataac aaactgtttt ttcgcttttt
     4741 ttgctaattg acttaaaatc gtcatgccca tagtttgtga gccgcgtctg ttgtttttat
     4801 acaaaaatac aatattagca ggcgactttt tttgttctgt gatatctgct atctcttttt
     4861 tttcttcccg ctttggaaat tggatcgctt cttttattcg tgacagcaag atatctgttt
     4921 ccaaaaagtg atccagtaca tagatttgac tttgaggtac tttttctctt gcaagacccg
     4981 ctagttcatg ttgcacgata accagctgat ttacttggtt attcaattca tagatagagc
     5041 tgtatccgac agaaagatcg attttttctg ctgctaattt ctttttcagc agggcggctc
     5101 ccatagcact tgatcccata cctgagtcgc aagcaaaaat gacggaatga atgatagatg
     5161 gttccaagat ttctcccctt tctttgaccg ttttgttctc tttttcttta tttggtaatt
     5221 tcaatagtat cgctgcacaa atgaaggtca caaatgtact gattgctatc cctatactta
     5281 ccccagctag ttgtggataa ggcgcattgg caaaaatcac gagtagtgac ccaggcgata
     5341 caggtgcagc taatcccgta tctaaccatt gaaatatcaa cgtgccgctt gccccaccag
     5401 ccattacagc taaaaataga tacggtcgca gcaagacaaa tggaaaatag atttcgtgaa
     5461 tcccgccaat cgcatggatc atcaatgctc cactcgcatt tactttttcc tgtttttctg
     5521 caaaacaaag gatagaaagt aagatcccta aaccaggtcc aggatttgcc tccatcaaaa
     5581 ataaggaaga gtacccgact gtttgtgaga attcgattcc taaaggtgtc aatacaccat
     5641 gattgattgc attattgaaa aatagtactt ttagaggttc taagatcaga ctgacaagtg
     5701 gtaacagatt cattgtgact agccaagaca caacggaggc gagcaacaag tgaacactat
     5761 ttaaaactgg ttcaataaaa acgattccaa ttatacaaat aacgctacca gtcagtccta
     5821 ctagtaggtt gctgacaagc atttcatatc cagccttgaa tttcacgtgg aatctagaaa
     5881 caaattgttc atatacgatg ccggcaagcg gcccgataat catcgctcct acgatttgtg
     5941 gagcattgga acgagcgatc aaccctaaag aagcaattgc accggcgact gccccttttt
     6001 gtccccctac cgatttcccg cctgaataac tgatcaagat gggtaaaagc agctggatca
     6061 tccaatggca tacctcttct aatgtggtgt gcaaatcgcc agacacacct tttgttgcta
     6121 atgacaagat tccccaagcg ataaacagac ttagatttgg catgatcatt tcatttaagt
     6181 gatgtccagc ttttctcagt tgtttcatag acacgcgact gctgttgttt gctttccatt
     6241 tgtattccat ctttttcacc atacttctat ttattgatgg acggatttta tgtagaaaaa
     6301 gcagcaaacg aaaaaccgtc tgctgctttc agctatctgg gaactatttg cctggttcag
     6361 tcgcaatgat tgctagttca ccttggaccg cccactcaga aacgccattt ggaataatac
     6421 agcttgttcc tttttctaat ggatatgttt ttccgtcaac gacaaggtct cctgctcctt
     6481 cgattaccgt catcaatgta taaggggctt gttttttgaa agatgtgatt cctttaatat
     6541 cccactcata tacattgaaa aactcagttt ctaaataagt gacgatcgat gaattccctt
     6601 ttcggacttc tttgatttgt aattctggtg ttttcgcagg gacagttgtt acatcgatcg
     6661 attgttggat atgcagctct cttgtttgtc cttgcgcatc ttttctgtcg tagtcataca
     6721 cgcggtaagt cgtgtcagaa ctttgctgtg tttctaaaat catgattcct ttaccaatag
     6781 catggatcgt tccgcttggt acataaaaga aatcaccttt tttaactggt actttcttca
     6841 aaaggtcatc ccagcgccca tctttgatca tctcagctag ttcttctctt gtctttgcat
     6901 gatgaccata gatgatttct gcaccaggtt cagcatcgat gatataccag cattcagttt
     6961 ttcctaattc accttcatgt ttcatcccat aggcatcatc tggatgtact tgtacagata
     7021 gatcatcttc tgcgtctaag atcttgatca ataatggaaa cactggttct gaaggatggc
     7081 caaacaattc cttgtgttct gaccatagtt catctaatct tttccctttg aattcgccat
     7141 tttctactac actgacacca tgaggatgcg cactgatcgc ccaatcttct ccgattttat
     7201 cgttagggat atcaaaacca aagactgaac gtaaacgact tcctccccaa attttttctt
     7261 gaaaaactgg ttttaaaaac ataggttcct gcataattca aactcctctc atttttcata
     7321 gctattatag cattatttcg gtaataccgt tttctctttc aaaaagtttt caacaatttc
     7381 atctcgacat atgagatact ccgcacgatg atcgacagga tgtacgcgat ttgacaaaaa
     7441 tatcaacgct tcttgtgttt ttatatcaat caagaaaaac gtacctgtat aaccagtatg
     7501 aaaaagaatc gcatgaccgt cttctacatc cttttttaag tcccatccta aagaacgata
     7561 gccatttttt ttcggtgttt gatcttgcag taaagaatca atcgtctctt cttctaaaaa
     7621 acgaccatta cggatttttc ccttgttcaa atacatttgg acaaaagtaa tgctgtcttt
     7681 caaatcaata aagagacccg cgttacctgc atgttcagcc aaaactcttg ctttcggatc
     7741 atgagtttgt ccacgcaaaa cctcacccga aggcagttgt tctgtgggaa cgattttttt
     7801 ggaaggaaaa ggaagaaata agctgttcat cattcctaat ggttccaata cttgttcttg
     7861 aaagatttcc actgcagttt cttggtaaat ttcctctagc atcaacccaa gaagaatcgt
     7921 accggcatct gtatatttta cttcttttcc tagttgctca cctggttcta gcttcaaata
     7981 agcggcgatc aattcttcct gagatagctt atctctgttt ggtatccatg ttttgatatc
     8041 agaagtatgt gtcagtaaat gacgcaaagt gattgtctga ttttcaaatt caggaagata
     8101 tttatgaagc ggttgatcga gttctatctt tccatcttct aatagacgta aaactaatga
     8161 agtggttgca acgactttcg tcagtgaggc tgcatcaaac aacagatctt ctgtcatcag
     8221 ctgcttttca ggaacgactt gggcaaagcc ggctgtaaat gtttctactt gattttcttt
     8281 tatgaaggcg actgctacac ctggataaaa tccttcttcc atctttttca aaataagctg
     8341 tttcgtgtgt ggatacatct ttttctcctg cttttctatc agattcttac agtccaagct
     8401 taaagcttaa tctatgagga atcaaccttt tttacacaaa aaaactgcgc gaaaaaccgc
     8461 gcagtttccc ccggtatact attttctctt tctatctcta gaataagcga tagtatgacc
     8521 gttcataaaa tgacgagaca tgcgacaatc gttaagaaag aaaaagttaa gcgtcgcatc
     8581 gaatgtcagt tttattcttt tcttttggct cactgacgaa ccaccattcg attcctatag
     8641 catcatagat tgtgagtact gtgtgctttt tgtctaagaa gaaatcaact gatagttgct
     8701 gcaagtgagc aatcagtcct tccatgcttt cagtatcgat catgaatttc agaaaatcaa
     8761 gtccaatcac ttcatctgta ggcaaatcaa ttgcgcctcc gctagcttct gtcaaaccaa
     8821 catggaaatc tccttcattc aatacatgga tgccctcttc ttcattatgt accttcaaac
     8881 caagtacatg attaagaaat tggcgttctt ttgttaagtt agtaacattt aaatggattt
     8941 tttcaaaata agaacctgtt gatagtttac tgaatttttc ttcagatttt cttaataatg
     9001 cttcttgatc cattgtttct ggaatttctc tttggtcgtt cttattctct tcatggtaaa
     9061 tttccaattc attctcttct gggtctacga gcaaaatccc catacgtcca tgatcttcta
     9121 aagcttcttt gattggataa ttcattttac ggatacgaca aaggatatct gccatttctt
     9181 ctgtgctagg gataatcaat gaaaaacggt tcagcttttt gatcaccccc atgtgatcat
     9241 tcgctctagg actttcttcc agcagcaaca tttcacttcc agcttcttct gttcctaaaa
     9301 tggctaattc gttttcttct cttttcaaat caaagccaac aacgttcttg taaaaatcaa
     9361 tcattttatc tcggtctttc acccgaatgg ctacactttt taaataggtg gcagccccca
     9421 attgaaaatc tttcatattt cttgctcccc tctacgtcaa gctttatttt aacgatggat
     9481 tcttcttctt tcaagcaata actttgattt tgttaaaaaa aatgtgagaa aattcgcatt
     9541 ttgcttgttt tttgtaaatt aatgagtaaa aactcttata aaaagaggtt gtgaaaaagc
     9601 tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg
     9661 gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtaaaa
     9721 tcagcggttt gacctgagta ttagagatac aaaacggaaa aatagcttct catattttgc
     9781 cgttttttga gctaataccg aaggacagct gattgaacac cgtttattcg gaaaaaggga
     9841 ctgtgacacg acttttgtca cagcccctaa aaaaaataat aaatttgtac tattctatga
     9901 agcttcttct tcagaaaact gactgttata aagatctgca tagaatccgt ctttttccat
     9961 caatgtatcg tggtttcctg tttctacgat cgaaccatga ttcatcacaa taatgttgtc
    10021 tgcatcgcgt attgtagaca agcggtgagc aacaacgaaa cttgtccggt tttccaatag
    10081 gcggttcatc gctttttgaa tcagtatttc tgttcgtgta tctacactag atgttgcttc
    10141 atcaaggatc aggacatctg gatttgctaa gaatgcccgc gcaattgtga ttagctgacg
    10201 ctgcccttgc gaaatattgc ttgcatcctc gtttaataca gtgttatatc cttctggcaa
    10261 tttacgaaca aaatcgtcta cgtgagcagc tttagcagcc tcaatgattt gttcgtctgt
    10321 tgcatcgtcg ttcccatatt tgatattgtc atagatactt cctgtaaaca gccaagtatc
    10381 ttgcagtacc attgaaaact gtgcgcgcaa ttcatcacgt gaaagatcac gggtatctac
    10441 accatcgtaa cgaatactac ctccagaaac atcgtagaac cgttcaagaa ggttgatcag
    10501 tgtcgtcttc cctgcaccag tcggacctac aatagcgacc atctcaccag gtttgacatc
    10561 caagttaaaa tcagtcatta gcaagttatc tggtgtgtag ccgaattgaa cattttcaaa
    10621 acgaactttg tatggtgtat ctttttcttc tggaaggcca gatttttcat tggacatctc
    10681 ttcttcatcc aacacttcaa atacccgttc ggctgaagca actgttgatt ggatagtgtt
    10741 catcaaactt gcaagttgag tgattggttg agagaactgt gttgtatatt gcaagaacgc
    10801 ttgaacatcc cctaatgtca tgttcccatt tgcaactttt actccgccta gtacagcgac
    10861 gaacacatag cctagatttt taacgaaatt catcaacggc atgatgattg ccgaaataaa
    10921 ttgagctttc caaccagcgg catacagatt ctgattttct ttttcaaata cttcctggtc
    10981 tttttcagta tgattgaatg ttttaacaat ggtatgacca ccatatgttt cttctacttg
    11041 gttattcaat aaacctaaac ttttttgctg atctgcgaaa aacacttgtg atttcggcgc
    11101 tacgatcatc acgatgatca agcttaaagg gatcatcaat aacgcgacta acgtcaattg
    11161 ccagctgatt gaaagcatca tcacgatgac cccaatcaat gtgactgcac tagttacaaa
    11221 ttgagtcaaa ttttgctgca gcgtgcttgc gatgttatcc atatcattga ttgctcgaga
    11281 catgatatcc ccatttgtat gtgtatcata ataggaaatc ggtacacgat tcattttggc
    11341 ttccaaatca tgacgtaatt cataaactgt tcgttgggaa acacgtgtca taataaattg
    11401 ctgtaaaaag ttgaatacag cagaaatcaa atacatcacg attactgtcg caatgatctg
    11461 cgcgatttta tcaaagtcta tcggaaattt tgttacttgt tggccttgtt gcatctgttg
    11521 agcgccagcc attacacctt tgaatatttc tgtggttgct ttccccaaga cctttggtgt
    11581 ttggatttgg aagatcacgg ctgctattgc tagtacaaat acagcgatga tcgcaaccat
    11641 acgtttagac atatagccaa acaatcggcg taccgttccc cagaaatttt taggcttttc
    11701 accttttaca ccgagatttc tgccaggtcc accatgtcct ggaccgcctg ctggtccatg
    11761 agagggacgt gtgtttgttt tactcatgct aagtcctcct ctcttagttg tgaatgaacg
    11821 atttcttgat aagtttcgtt tgttgctaat agttcttcat gtgttccttt gccaaccagt
    11881 tttccttcat ctagcacaag gatcatatct gcatccatta ctgtacttac tcgttgagca
    11941 actaaaacgg tgatactttc tttcatattc gttttcaatg cttggcgaag attggcatcc
    12001 gttttgaaat ccaacgctga gaaggaatca tcaaacacat agatatctgc tgttttgact
    12061 aatgcccgtg caattgccag acgctgtctt tgtccaccag agaagtttcc tcctccttgt
    12121 tccacatggc tatctaagcc atcttccatc tctgaaacaa aatctttagc ttgtgcgatt
    12181 tctaatgctt tccaaatcat ttcatctgtt gcatccggct taccatactg catattgtct
    12241 cggatcgttc cagagaacag aactgctttt tgaggaacaa agcccattaa ttctcgcaaa
    12301 ttatattgag tcatgtcact aatgtccgtt ccatttattt ggatcgaacc agattcgatg
    12361 tcatataatc gagggatcaa attgactaag gtcgttttac ctgaaccagt cccccctatg
    12421 attgcgacga tctcgcccga cttggcttgg aaatcaatat cttccaatgc aagtttttct
    12481 gctccatgat atcgataatt gacatgattg aaggccaatg ttgcgttttt tccagcaaaa
    12541 gaaactgttt taggattttc agggtcatgg ataccgattt tttcatccaa cacttcatta
    12601 atacggtcag ctgaagcttg tgcacgaggc accatgataa aaatagcgga aagcatcata
    12661 aagctgatca agatctgcat cgcataagtc ataaatgcaa tcagattacc gacttccaaa
    12721 gtcatatctg ctatgtagtg tccaccaaac caagtgattg ctacgtttgt accgctcatg
    12781 atcaatgtca tcattggcat catcaatgcg acgatcgtat taactttgat tgccgtattt
    12841 gtataatctt tattcgctaa atcaaaacga ttttcttcaa agcgtgtctt atcgaatgct
    12901 cgaatcactc tgacccctgt caatccttct cgaaatacca ggtttaaacg gtcagttttc
    12961 ttttgcatac ttttgaataa tggaacagca aagtacatga ttccgccgat caaaataatc
    13021 attactggga tgacaactaa gaagattttt gtcaactgat gatctttttg ataagcaagt
    13081 atgctcgcgc caatcaacgt gattggtgca ttgatcatca tacgtaaaaa catttgtgta
    13141 accatttgga tttgagtcac atcattggtc gttcttgtga tcaaagaggc cgttccatat
    13201 ttatcaaacg catcttttgt cagactctct gattttttat agacatccgt acgcaattgc
    13261 ttacccagtt tctgtgattc acgagtagca aaaaatgtat ttgcgatagc agctaagata
    13321 ctgatcaaag aaaagccgat catcacaaaa cctgtatgcc aaatataatc gacatctcct
    13381 tgcgctaccc ctttatcaat aatattggag gtaagtgtcg gcaaatataa atctgccagt
    13441 acttggatca ccatgaaaac tgcagctgca attgctgaag tcaaggagat ccgttttacg
    13501 atttttagca tctagttccc ttctttctat ttatatactc cgtttttcag ccaagaaagt
    13561 ttcaaattga attcactgat tgcttgctct actgagtaat cttcagatgt tgaaaataag
    13621 cgatacgaat gcgcaattgt tgtaaataca gtattcatca ctaattggat cagtaattcc
    13681 aattcatgat cattttttac tttcagcttc gtccgatcga tcaattcaag caactctttt
    13741 gcatcttgtt tgtgttcttt atgtggatcg tgaggcgttt tttggcgact cgtatgagga
    13801 gcgacttttc ttgaggcacg gtaatccatg ttcataaaca agttacgata aaaagaagca
    13861 ttctcgcctt ccaaaatctc ataaatcatt ttagaaaaat aggattcgaa tcctttgaat
    13921 agatcaccat cggattcttt cattagttgt aacatatctt tagagctgtc acgtctcatt
    13981 gtctcaaagt aataaaaata aagatcctct ttatcttcaa aatactgata gaaactgcct
    14041 ctagggatat ccgctaattt gacgatatta gcaatcgaag cttcttttaa gggtactcta
    14101 gaaaactcga ttctcgcagc ctcgattagt cttttttgtt tttctttagg caaatggaaa
    14161 aatgtctgtg tcggcatatg gatcctcctt tctttcaaga tgacacattg tcatatgacg
    14221 ctgtgtcatt ataacacctc ctacctgttt tgcaagacat ttgcatagtt tttttcatta
    14281 tcatccgata cattaaaaaa gtgataagag gaatccttcc atttgaatta atgagagttg
    14341 cccattaaaa tagccacata ctaaacaaag gatgagataa atggcccaat caattagtgt
    14401 ccaagacttc aataaattgc ctctcgatgc tgacagcatc gttttagata caagaaatac
    14461 cttagattac acaaaaaacc acttaagcca ctcgctttct atccccgctg ctatcttgcc
    14521 aagacaatta aaaaatctag atccaaagaa actttattat attctaagct atacaggcag
    14581 gcgatcagaa gcattagccg atcaacttac tgctaatggt tttcgtgctg tttatatcat
    14641 tggtgggatg gaagctcttc agtcgagtgt tgcttgaata ttgcttgtta gattcaaaag
    14701 aggctgtgac aaaaaatcgt gtcacagcct cttttatacc ctgtcttttt ttagttattt
    14761 attcttcaac cttatcaata tgacttgata gccttttctt tttctcatag ttataacaat
    14821 catagcaata aaaggagaag gtaagcatat ggcatctgtt ttagtagtca aaggtcatcc
    14881 cctaactgca gaagaatcgc gcacagttaa ggcattgacg tcttttttaa caagttataa
    14941 agaaaatcat ccagatgatg aagtgactgt tttggaactt tatcgcgatg atattccgga
    15001 aatcgatgaa gaattattat caggttggga agcattacgt gctggtgcag aattcacttc
    15061 cttatcagaa agtcaacaac aaaaaattgc tcgtttcaat gaattgactg atcagttcct
    15121 tgctgctgat aaaatcgtta tcgcaaacgc cttatggaat ctaaatatcc caacaaaatt
    15181 aaaagcttgg ttcgataccg tcaatgtagc tggaaaaacg ttccgctata cagaaaacgg
    15241 gccagaagga ttagtgacag gtaaaaaagc actgcacatc caatctaatg gcggtgtata
    15301 caacggacaa gactttgcct ctcaatatgt aaaaggtatt ttaaactttg taggcatcga
    15361 tcaagtagat cagctattca tcgaaggaat cgattatgac cctgaccgtg cagatgagtt
    15421 gatgcaaaat gcattagata aagctgctgc tttaggtaaa tcattttaaa aattgagata
    15481 aatgcataca aaagagacag aggaaaaaat cttcaaagca gcatttgtac cttggatatt
    15541 ttcccatctg tctctttttg ttattctttc tgctttttca acggattttc ttcataaaga
    15601 gaagagattg ttgtgtacca ttcaaaaaca tgagtaatca ctactttagc tgctgcataa
    15661 acaggaatcc ccaagatcac accgatgatt ccgaacaatt tcccagaagt cagcagaact
    15721 aataaaatcg ttactggatg gattgctaac tgattcccta gtactaatgg agagatcaag
    15781 cgtccttcta ttgtctgctc tactgcaaaa acgatcagta ctttgatgat catgaatgga
    15841 cctgctacaa tggctaaaaa gatagccgga atcatcgcta aaaatgatcc taagtaaggg
    15901 atcaggttca aaaaacccgc aataatcccc aacgtgacag catactctaa accaatgacc
    15961 gcaaaaccaa tcataaacat gattgccaca gcaaaagcca cagttagttg tcctcttata
    16021 taagaagaaa cttgactatt catttcactc agcacattca acgtcggttt ttgcatgcgt
    16081 gtcggtaaaa atttgacaaa ataaggtgct aattgtttac cgtctttcaa tagataaaat
    16141 aagataaacg gcatcgtcag cagagccact aaaatagtcg ctaccgcgcc gacaaaactt
    16201 ccaagactct gaactgtact cttagatata tcttggatca tatcgcctgc cgaattcatg
    16261 aacttatcac ctgctgtttc caactgttcg cgaaattggt tgaacagcgg gtcccgcaaa
    16321 atctcggtta atttattatc aatcgtatct acatattgag ggaagttact gatgaagctc
    16381 actgtttgtt cctgaatctt agggatgatc actacgctcc cccatgcgat tagtgcaaca
    16441 accaaaacaa acagcccaat gatactgtaa agccttggta cttttcgctt ttcaaagtaa
    16501 tcaactaccg gattcatcaa gtaatacaag attcctgcta aaatgatagg cagacctacg
    16561 attgctaaaa attgccagat tggtgcaaac aaatatgaaa cttttgtaaa taaaaagacg
    16621 atcagcaata ttaacaatac aacaagtaag gctgtgacta cttgattatt taaaaaccag
    16681 cgccaaaacc acgataccgg tctgttcttc ttattttttc cttccataat tcctcctgtt
    16741 agttataaaa aatttggctt cccaaaccaa tagcaggcag tctatgcgga tgaagataga
    16801 ttccatttgc aatttgtcct tcttctggac aagcatcaaa aatcaatgat acgtgagcta
    16861 ctgtctctaa attcccattg gccatcggac cgacataatc gatcgtatat acctgatcat
    16921 caaaagcaat tgttccgcct gctttcaaag aaaaaggttc gtcttttgat acttcttgaa
    16981 tcaccgaata ttttcgtaag gcagctgttg ctgttttatc gaacaaaatc agcatgggtt
    17041 cttccttgtt tattgcttct gtcccaattt ctgtaatctt tgcctgaatc attgcactct
    17101 ctcctttaat tgctttcaaa aacagtataa cacattctca ttctttagcc gtgtggaata
    17161 attatgtgat agaataaaaa cgaaaactat ttcattacaa ttaggaggaa tcagctcata
    17221 tgatcgaaaa gataatttta ggaacctata cgcgtcgcga aagtaaagga atctatacga
    17281 tcgaccttga tacagaaaaa gaagaattag ccaacctttc attagctgtt gaagaaaata
    17341 gccctaccta tgtagcaaaa agtaaagctg gcaatcttta cacagttact agtgtggatg
    17401 gattaggtgg tgccggtgcg tacgataagg attttcattt tttgaatgcg gtaacagagt
    17461 ctggcgctcc tttgtgttat gtagcggttg atgaagcacg tcaattacta tacggggcga
    17521 actatcataa aggtgaagta aatgtctacc atatattagg agatggcgaa ctgaaagcag
    17581 cagattcaat attccatcaa gaagcgacag gtcctcataa aaaccaagat catgcgcatg
    17641 tccactatac ggatctgact cctgaccaac gattagtcgt atgtgatctt ggtacagatc
    17701 gtgtgtatac ttatgatgtg tccgaaaacg gcaaattgaa tcttgtaact accttcactg
    17761 cagagccagg aacaggccct cgccatcttg tctttcatcc aaatggacaa ttcgcttatt
    17821 tatttggtga attagacagc acagtccgcg tgctttctta taataaagaa acaggtgctt
    17881 ttgaagaatt acagaaaata tcgaccttgc cagaagaatt cgacggtgaa aatggagggg
    17941 cagctatccg catttcaaat gatggcaagt tcctttatgc ttctaaccga ggacataact
    18001 cgattgctgt attcgcagtt tttgaagatg gttcccacat tcaaaacaag caaatcatct
    18061 caacagaagg ggattttccc cgcgatttcg cactagatcc aagcaataat tttgtcgtgt
    18121 gtgccaatca gaacaccgat aatctgacat tatatcgccg tcatcctgaa acaggattac
    18181 tggaattact gaaaaaagat gtctatgtac cagaatgtgt ctgtgtttat tttgatcgtt
    18241 agtaagaaaa ggagaatgaa atgaatcagc aagacgtatt agacagacta cgggaagaac
    18301 taaatattcc tttctttgat gggaaaatcg aagataaaga gtatagcgaa gaggaatatc
    18361 agaaactcaa agccgattta cagaactact ttgaacaata tgtacagaat gtagaaaact
    18421 aatcagcttt ccatactatc tgaaaagaac atcaggacaa gacttttccg tctatcctga
    18481 tgttcttttc tacaaataaa aaaccgccga gatggttaga ctcgacggaa aaggagtatt
    18541 tactcgattt tgagtaagta caaggttgtt gttggttata ggtatgtcat tatcataaag
    18601 gattttgatc actctatcat aaaggtaata ttacatttat attagataac tctagttttt
    18661 attgttatca aagatttctt ttattttttc aacaacaaat tagtcatttt ttggatggca
    18721 gctgtcaatt ggtctgtcgc ttggtcttgt tttgcttcag atgtattgaa tagatcttca
    18781 tctacccctg attcaagatc gatactttgg atcatttctt cacagcctgc tacatatgaa
    18841 agataagctt tttcaaaatt tttatgaatg cccattgctt ttgctggtgg acgcaaagta
    18901 ctgactttct taagcattgc ccgatatttt tttgttcctt cttcaaataa caggatgatc
    18961 tcttcaagag tcgatttgtc cagttcactt gtttttcctt catcaattgc gtttctgatc
    19021 acttcatatt tcggatgcat tccttctccg attgcttctg tttccttgac gatttgatcg
    19081 atggactgca cataatagcc aatattcggt aacatgttcg ttttcctact ttctccattg
    19141 ttgttattat ttattctact tgtttttatc ctcaaatgac aagtacttga aaaaatttaa
    19201 tggtttctat acagctcgcc attcttattc gtgctcaaag aaaccatctg ctaaactata
    19261 ttgtttccct gcgacaatgt catattggat caattgatat tcgtcgtaaa tctcttgttg
    19321 cgagcgattg aattgcgctt ctttatacca ctgtccgttt tgatagtaca gttctcgttc
    19381 aaacgccgga agttcctgtt ctaatgataa aagcagctga taaaagccat ttgttgtctg
    19441 attcgtctgt tccattagat tcgctgcaaa ataaaacgga ctggtgattg catcgtgagt
    19501 cgtttgcttt tctaattctc ctttactgtc gaacataaga aattgtgttt catgcaatgt
    19561 ttgtttgtca ttttttgctt ggatcgtatc tgaatagatc cctggaagat gatcccccca
    19621 aaaaacaact aaagtccttc tagaaagatt cttcaattct tccgtgaact gcttcagtgc
    19681 cgtgctgcta taggaaatat cttgcagata attctctaag tcaagtgtcc cacttccatc
    19741 tgaagttttt gcaggatagt ctagattatc atactttcca tcgtagggca tatgtgtctg
    19801 catcgtgact aaatgaatga actgaggtgt tttttcttcc ttgagaagtg tcattacttc
    19861 tttgtaagct gactcatcag aaatatatgg attattctct atcgtatccg tataggtcat
    19921 cgtacgctcg ctgatgaact ggtcaaatcc taatgtctga taaacatcct ctcttttata
    19981 catcgaagta ttgtatggat ggatcgctgt cgtctggtat gattgtgcgt ttaacgctga
    20041 aacgatagaa ggaagctgat ccatttttgg tacaagcatc gtgtaagggg tggtgagttg
    20101 cgcattaaat aacgccatag aaaaaccagt caatgcttca aattcgatat ttgctgttcc
    20161 accgccatag ttttgagaaa gcatattgcc actatatgtt tgatcagcta cttcataata
    20221 atctgccaat ggctcaccag atacttctac gccgtttaat cgactaggat ctgagaaact
    20281 ttcactcatc acgaacacga tgttcggcga ctcttcctct acagttttat ttttttcatc
    20341 ggctagcttc tggtattttt cagtgatttc tttgatcttt tctttcgaat atccttcagg
    20401 ttcatccatt ggttctacct tcagattgta caaaaaccca ccgataaaac cagtgttata
    20461 gtagttcatt ttctggctgt aaggtatcca tagtgccgtt ttattatatg cttttcgcaa
    20521 caaattatcg ggattgttga aattactgat ataccctagc agtccaattg ccacaagcag
    20581 tgtcgttaat cggatcaatt ggaatcgcct tccttttttc aggcttcgaa acatgtacca
    20641 gcaaaacaat cctatcacgg ttcctgcggc agctaagatc actgtaaaca gcatagtgcc
    20701 ggtcatatct ttcagcaatc cgatttcagt gatcatcttc aaatcgtctg gataaatcgg
    20761 ttcttgccga taactcattt tcatataatt tgcaaaacct aatacaccga tggctacgac
    20821 atacgtaagg gaacctaaag ggacagatcc tgctagtgca atcagaaaaa taagaataat
    20881 cagcaagacg agacaggcta agaagaattt ttccgtatgc caagaaaaag caaatttcaa
    20941 cgcaaggtct acggatagat cattttgaca ccactgcaaa aataaattac taaaaacaac
    21001 aagaaaaaag atactgatga ttgctgctgt tccttttaaa atctttttct gtttggtcga
    21061 aagtctgctt atatatcttt tcaaatgctt gctccttcat ttctatcaat aaaagaatgt
    21121 tgcttctctt attttagttg tacgaaattc gaaagtaaag aacggaaaga agaattaatg
    21181 aaatccaaat attctctttc tttatggcct ttctggtttc tttaataagc ggacctttcg
    21241 tactccagaa attttttcta gctgttgttt cacttgattg agctgttcaa aatcagtact
    21301 gtctatgtca atcaatatgt agacatgctc attcttatgg ttacgcgata tatctgcgat
    21361 cgctagctcg ttctcattca gcagagaaaa tatctctgct aatatattgg ttttcttttg
    21421 ataaaatata gtgaatcgaa acggcgcttg gaaaaacatt cgtgcggatg gataattgac
    21481 cgcttctcta accgtaccga acaaaagata atcttttaag gcttcaactg ccaaacgtcc
    21541 gctgtctgcc aaagcttcct gagtcgatcc ccctagatga ggcaacataa gtatacggtc
    21601 attttctaag aactcttctt ctggaaaatc agtcaagtat ttagccagat gttgtttttc
    21661 caatgcttgc ataacagcat atttatcaac aatttcactt cttcctacat tgataagtac
    21721 agcatctttt ttcatttttt ctatattcgc ctgatcgatc aatccttttg tatcttctgt
    21781 caacggcagc atgactacga tgtaatccga ggtagaaaga actgtttcta aatctgcttg
    21841 ccgaacataa tccaactggg catcgcggat agaataaccc aagacatcca tccctaagct
    21901 atagcaactt agcgctactt ttgtgcctat cgctccaaga ccaagaagac cgatggtttt
    21961 gccttgcaat tctcgtccca cataagcact tcttttgttt tctgcctgtt ctaaaatatt
    22021 aggacctgtt agtgtttgta ccatacgact tgcttcgatt atcggacggg aagataagag
    22081 caaacagcac aaaacaagct cttttactgc attagcattt acacctggtg tattcatgac
    22141 gatcgtcccg ttttcagaag ctttttcaac gttgatcgtg ttcaccccaa caccagctct
    22201 tgaaattgct agcaaatctg gtgtaagcca ttcatctttc actttcgtac tacgaataac
    22261 tattccttcg gctggcaaat catctattat tttgaaatat tccccttgta acttttctat
    22321 ctcttgttct ggaatagcat caatcaaatg aacgtgatac ataaaacccc ctccttacta
    22381 acaagtatct cgaaatttct gcaattagac aactgtttac catttgactt cctagaagca
    22441 aaaagagcag ttagagcaga gtaggctgag tgataaaaat aaggctatga caagttactg
    22501 ccataagcct tattttcgag agcaaaatat tgagggtgag atatcttgat aaatggtgtt
    22561 ccgttagctg acattgataa taagactaaa tttcaaaaaa gttgaaaagc aaatttcgtc
    22621 aatctcttct tatttcttaa aactaagtgg ctttttcaca acctcttttt atctaataac
    22681 cactgtttcc aactcaacta attttgccca cgtttcaatt tgatctgttg ttagatttag
    22741 cgagacaacg gtatgatgac cgccacctgc ctgaatccat gaatggacac cttcatggaa
    22801 attcggttta gttttccata acacacgtgc aacaggtaaa ttaggtgctt cagtcgttgg
    22861 ttcaaaggct tctacttcat tgatcagcag tttataatgc gtaccgaaat ctgccatcga
    22921 tacgactacc ccttctcctg ctttcccatc aaatacaaga cgcgccggat cttctctatc
    22981 tcccattgat aaaggagaaa caacgattct tggtttcgtc gcagcaagcg tcggatctac
    23041 ttccatcata tgggattcca agattgcttc ttggccacta gctagctcgt aagtataatc
    23101 ttccatgaat cctgtatctt tccctcgagc catgattttc agcaaccgat caatcgctgc
    23161 agttttccag tcgccttccc cagcaaaacc gtatccttct gccatcaaac gttgtacagc
    23221 tagtccaggt agttgtttca ttccatacag gtcttcaaaa ttagtcgtaa aagcgttata
    23281 gcctccttct tctaagaaac ggcgaatacc gatttcctgc tgagcttgta cttttacatg
    23341 ttcctcccat gtcttttctt cataatcacc atagtcgaag gtatacaatt ccttgtactc
    23401 ttcaaataat tgatcgactt cttcgtctga tacgcgatcg attacttgta caagatctcc
    23461 gataccaaaa taatcaacag tccaaccaaa ttgaatctgt gcttccactt tgtctccttc
    23521 tgtgacaccc acgtttctca tgttgtcacc aaaacgagcc acttttattc caaaactttc
    23581 attataggct actgcaacat ccatccaatc agcaatctct ttttggatct caggtcgttt
    23641 ccagtagcca acgacgattt tattttgttt atttaatcga gcattgatga acccatattc
    23701 gcggtcacca tgagcggatt gatttaaatt catgaaatcc atatcgatcg ttttccaagg
    23761 aatgctttcg ttatattgcg tagctaaatg aagcaatggt ttttgtagaa gctttgtccc
    23821 gcggatccac atttttgctg gtgagaaagt atgcatccaa gtgatcacac ctgctacttc
    23881 ttctcgataa ttgacctctt tcatgatttt tgtgatcgta tcagcagtaa ctgctaattc
    23941 ttgaaggcgg attggataag gcaattggcc attttcattc agttcatcga ccatttcttg
    24001 tgcatgtttc tttacttctc gcagtgcttc ttccccatat aaatgctgtg atcctacaac
    24061 aaaccaaaat tctttttctc caatattcaa cataattagc cactcctttt atttgttttg
    24121 tccgtaatac gcattttttc cgtgttttcg taaataatgt ttatctaaaa tccgttgagg
    24181 cagctcttct gaaaatgaat tgagctgtct ggtaaataaa ttcatcttgc atacttcatc
    24241 tagtacgact gcgttcatta ccgcactttg cgcatctttc ccccaagtaa atggaccgtg
    24301 tccgtgtaaa agtacccctg gaattgctaa gggatcaatt ttgcgttctt tgaatgtttc
    24361 tatgataact tttcctgttt catattcgta cccgctgtcg atttcctgtt gtgtcaaaaa
    24421 tcgagcacaa ggtaccgatc cataaaaagt atctgcatgc gtcgtcccca tcgcaggaac
    24481 atccaatcct gcttgtgccc agatcgtcgc ccatgtcgag tgagtatgga cgattcctcc
    24541 gatttgagga aatgaacgat ataaaacagc atgagtaggc gtatccgaag agggattgag
    24601 tttaccttca atgatatgat tgtccaagtc cacaacgacc atgtcgtctg ctgtcatctc
    24661 ttcataactg actccgctag gtttgataac gaaatatcgt gtttcaggat caaaagcgct
    24721 cacatttccc catgtatatt tgacgagtcc gtgtttaggc aagtcaagat ttgcttggta
    24781 tacttcttct ttcagttgtt ccagcatttt ctatcactcc tttttcttgc aattgatgaa
    24841 tcgctgtttt ttcaatgtcg atcccctcac ggtaacgtct cataaatatc tgatagcctg
    24901 ctatttcttc tgctgaaggt gtgatggtga ttccttggtc ttgtccaaac acgtcttctg
    24961 ccaagaagtt ttctaaagac ttcccatgtc gatccatcat ataggctgct aataacgcga
    25021 ttccccacgc tccaccttct ccggctgtgt ccatcactgt aactggtgcc tccatagcag
    25081 atgctagaat ccgctgtcca acttctggtg ttttaaaaat ccccccatgc cccactaaac
    25141 gatcaatttg gacattctca gatttcaata tatccattcc gattctcatt gcaccaaaag
    25201 cactggctaa atggatccgc atgaaattcg ctaaatcaaa acggctacct ggtgtacgta
    25261 caaataatgg ccttccttca tttactcctg taatgttttc accggaaaaa taaccataag
    25321 aaagtaatcc cccgcaatcc gtatcgcctt ctagcgcttt gttgaataat accgtgaata
    25381 atttatctct gcttatctcc atacccaaag aattggtaaa ttcttcaaaa atactgaccc
    25441 aagcattgat atccgaagag cagttattcg tatgaaccat gcctactagt tctcctgaag
    25501 gtgtagtgac catgtcgatt tccggatgga cattttctag ttctttttct aatacgatca
    25561 tagcgaaggc ggaagtacca gccgaaatgt tccctgtccg tttggctaca ctgttcgtag
    25621 cgaccatacc ggttcctgca tccccttctg gcggacaaac cgggattcct ggtctcaaat
    25681 ttcccgtagg gtcgatcaga taagcccctg attcgcttaa cttgccagct tcttctcctg
    25741 ctactagaac tttaggcaaa atgtgatcca agtcaatcgc tacgtgcgct ttttcgatta
    25801 actgaccaaa aatatttttc atttcttgat ggtaggtttt cgttgaagga tcgataggaa
    25861 acatgcctga tgcatctcct acgcctagta ctttctcacc agtaagctgc caatggacat
    25921 aacctgcaag cgttgtcaaa aaattaattt ctttcacatg ttcttcttta ttcaaaatag
    25981 attgatacaa atgagcgata ctccatctct gcggaatatt gtattgaaat ttttccgtta
    26041 atattcgttc tgcttcttca gtgattgtat ttcgccaagt acgaaatggt actagtaact
    26101 gttccttgct atcaaatgct agataaccgt gcatcattgc actgaaccca atcgacccga
    26161 ttgtcgtaag attctcgcca tagatttcga atacttctgc tgccattttt cggtaactga
    26221 cgcgcagtcc tttccaaata tcttctaatg aataggtcca gataccattt tctagtttgt
    26281 tttcccactc ataactacca gcggcaatag gagtaaaaga tgaatcaatc aatacagctt
    26341 tgatcctagt tgatccaaac tcaatcccta aagatgtacg accatctatg acatctaaca
    26401 tactttgcgc atttacttcc ttcataatga tttaactccc tccttctcgt caaacctgat
    26461 caaaccgtct ttttagtagc gcttacaatg attattgtat acttttgtac gtacatgtca
    26521 agcggttatt tacaagaata caataaaaaa cacttgtttt gtacgtacat aagttataaa
    26581 atgaaattgc ttacaaaatc tgatcaaccg ccttctcgaa attctagaag gagagaatac
    26641 ttatgaaaaa agaaaaaaaa gtttcctcat cgtttattta tttttttggc gcatttggcg
    26701 gcattctatt tggatatgat attggtgtta tgaccggcgc cttacctttt ttacagcatg
    26761 attggggttt ggcaggtaaa gcttctttga taggatggat cacgtcttcg gtcatgttag
    26821 gtgcgatcct tggcggctct ttatctggtc ttctttctga taagcttgga cgacgaaaaa
    26881 tgattcttct ttctgcactt atttttatgg ctggttctgt cctttctgca tctgcccctc
    26941 acaatggttc ttattttctg attgcagcaa ggattcttct aggtttagct gttggcgctg
    27001 catctgccct agttcctgct tatatgtctg aaatggctcc tgctagacta aggggaagat
    27061 tgtcagggat caatcaagtg atgattgctt ctggcatgct cctttcctat gttgcagatt
    27121 atttgctaaa agtgttgcct gaaacgatgg cttggcgcgt gatgttaggg ctcgctgctg
    27181 tccccgcgct gattttattt ttcggtgttt tagctctacc agagtcaccg cgttttctta
    27241 tgcaatctgg ccgcttggaa gaggcaaaaa gagtattgaa ctatattcgg acaccaaaag
    27301 aagctgaaca ggaattcgaa caaatccagc tgaatgtcaa acaagaaaag acaaccggaa
    27361 cttcttggca tactttgttt ttagaaaaat atcgttcctt agtcattgcc ggtattggtg
    27421 tagcagtatt ccagcaattt caaggagcta atgctatttt ttactatatt ccgctgatcg
    27481 tagaaaaagc gactgggcat gcagcgagcg atgcattgat gtggccgatc atccaaggaa
    27541 tcattttagt tgctggttct ttgctctttt tggttattgc agacaagttc aatcggcgaa
    27601 ctttattgaa aataggcggt agcgtgatgg gactttcatt catcctacca gctgttttag
    27661 gaactgtact ggatgctcat acaaattcgt tgttgattct cttgttttta tgtatttatg
    27721 tcgcatttta ttcttgcaca tgggctccac tgacatgggt aatcataggc gaaatattcc
    27781 cactagctgt ccgcgggcga gcatctggcc tagcctcttc ttttaattgg attggttctt
    27841 tcctagttgg cttgcttttt cctgtgatga ctgcttctat gtctcaagaa atcgttttcg
    27901 gcatttttgg gatcatctgc tttttagggg ttttatttat tcaagagatc gtaccagaaa
    27961 ctcgtgggaa atctttagag gaaatcgaac aaagtgcatc taaaaaaact tatcctaaac
    28021 gtatttctat ccatcattct taaagaggtt gtgacagaag tggtcagctt caagaaataa
    28081 gaaggaaccg cttagtttcc agtcacaagg aacaatttca aaaaacagct tctcatgttt
    28141 gatgaatatt gtgacttgtg accagttggt aggagaacac cgtttagtgg agaggtggtg
    28201 aaagaaacgt tcagccttga gcgatgacaa aagctctcac aaacattgcg acgagtaacc
    28261 gtaggagcaa taagaggcgg taaaaacccg tctcttattt ttcgttttct ttcactgttt
    28321 cccgttcaac tagtacaggt tcatagagaa tatttggctg tgcctgtcct ttttcaatcg
    28381 ttgtgacgat ccaccgtgcg gcatcttgtc ccaacttttc ttttggatgc gtcaatgttg
    28441 tcaatttttt attccctaaa tggcttaaag aagaatcgtc tgttcctacg atcgataggt
    28501 catctggaat atgatagcca ttttctgtca acttatctag cattaaacta gctatttgat
    28561 cgttatagca aatgatgcca tctaaagctt gttcttcttt caaccgttgt actgtttttt
    28621 cagcgattgt ttctcgtgtt tccgtggtga aagtaatgat atcttccgtc aaaaaacgca
    28681 cattatactg ttcacacgcg ctgataaaac ctttcatcct gtattttcct tgaagatcgt
    28741 cgatttttgt gatgaacaac agacgctgat gtcctttttt tatcaaatat tctgctgcca
    28801 taaaaccgac tttcacatca tctaaacaaa tataagaggc atttaactct tcataagcag
    28861 cattgatcat caccatcgga atgccttgtt ctctcaaagc aacataaagt gctaagtttg
    28921 ggttatactg attacttttc gtaggctcaa cgatcaaacc atctacccca taagcaatca
    28981 ttttcttcaa acactctttc tccaattcgt gatcgttatt cgtactggct aaaagcaatg
    29041 aatacccaga ttctcgtaat tcattttcga ttcctcggat aatcgacgga aaaatataat
    29101 cagagagata tgtggtgatg acaccgatcg tttttgttct gttcaatggt gttttttctt
    29161 tcttatagct gtcatctaca tatgtgcccg accctttttc tttacgcaga tagccctcat
    29221 ttaccaacac agcgattgct tgccgaatcg tatgtctgct gacgtcatat atttcttgca
    29281 gcttgaattc tgagggaatg gttgtattta cttcgtattc ctcacgaagg atcttttgtt
    29341 ttatctcttc tgcaatatct tgatattttg ccatttgcct tgcccctttt tatttgtacg
    29401 tacatatatc ataacgagtc tgcaaggttt attcaagcaa aaatcaaggt ttcttgtgct
    29461 cataaggcat acggaacagg ttcaccaaat tctggaaatc cttgctcatt ccacgtaaag
    29521 cattgcgcgt tcgcgtgccg attaggattg tctaacggat cccctttttg gttcttctct
    29581 ggtcgagcat gatagatcaa aatatcaaag cacccgtctt ctgaaaccgt aaacgagttg
    29641 tgaccaggac caaattgctt atttttttca gaagaaacga atactggctc cgataattta
    29701 tgccatgatt cttttaataa aagattcttg tgctcatcag cccacagcag gcccatggca
    29761 tagttttcat ctgttgcact accagaatac gtaatgaata ttttcccatt gcgagtaagg
    29821 actgctggac cttcatttac tgaaaaaccc cgtttttccc attcatactc aggtatagaa
    29881 aggaggatct gcttcccctt taacgtccaa ggattatcca tttcggatag ataaagatta
    29941 gaattcccag taatttcagg atcttgttgg gcccacacat aatatagctt tccttgagaa
    30001 gagaaagttg ttccgtctaa actgaaagtt tcccttttcg tttttatttg tcccttttct
    30061 atccaatctg tattcattgg atctatatgc tgattttcaa gtacgaacat gcggtgatga
    30121 tgagattcat cacgtatagt tttatgattg cttgcagcaa agtaaatata ccattttcca
    30181 ttgatataat ggacttcagg tgcccagatc aattcactca tcggtccttc ttcatgagcc
    30241 tgccatacaa tcaggcttgc agcatgttct aattcattca gtgactttgc ccttcttaat
    30301 tcaatcactt ggtagcctgg aaccgatcct gtaaaataat aataaccatc tgtatgttta
    30361 tatacccaag gatctgcccg ttgccgaacg ataggattat tataaagtgt cttcatcata
    30421 acaaatttcc tcacattttc tttattctaa ttacattcca agagtatttt tctatcccta
    30481 catttaattc gtcatgtttt tctattgaaa aatagtttat ttcaggtttt attttttctg
    30541 gttcttcacg tgtatttttg tccaacagat cgctactggt taatgtgtag tgctctcctt
    30601 gatctagcaa attgaaatca tgttcaatcg ttatcttttg ttgttcattt gtgcgattga
    30661 tcaagaaaat agtcagctga ttgccgttat ctacgatcac actgtccaca taaggaacat
    30721 tttttatctt ttcacaatca taaacagggc aaatgatttg ttgtttcaat acttgacctt
    30781 tagcatattt cgataagtgt tgcatcacat aataagttgg attcctccat gcttgtcctt
    30841 tagcatctgt cagtattaaa ggtattgtgt ttactaacaa tgattgacaa ctaatcttta
    30901 cgcgatctgc gtgtcgtaaa atagccagcc ctaatgaacc aaataataat gtatcttcca
    30961 tagtaaagta acaccaatcg atgggactac cgacttcaaa atccctatgt cttttttccg
    31021 gttgggcatg tacattccat tcatcaaacg ctagatacat tgttttctca cttctcaaat
    31081 atccttttac ataatcacaa gtagcaacga tttcttcaat ttgacgatcg aatcgataag
    31141 cttttgcaag gtatgtcgct gtatcatcac gctcattttt aggaggttta gtcaagtctt
    31201 cttcatcata cttatcaata tagttatgca aagctaaata atctacattc tcataagctt
    31261 cttctaatgc gatacgatcc cattcagggt acgatggtaa acgtggcgta gagcttccaa
    31321 ctaagattgt ttcaattgaa tcatccacta acttcatgac ttttgaggct tcatttgcta
    31381 acctaccata ttcataagca ctcttagccc ccacttgcca tggaccgtct agctcattcc
    31441 ctaaacacca taatttaacg ccaaaaggct ttttttgccc attttttatt cttaaatcac
    31501 tccagaaggt tccttttgga aaattacaat attctactaa attttttgct tcatcaattc
    31561 cttttgttcc taaattcacc gtgaatatcg gtttcgcttg tacatgactg atccatttaa
    31621 aaaattcgtg gagtccaaaa gaatttggtt ctatacctcg ccatgctaaa tcaatttttt
    31681 ttggtcgttg ttctttccta cctacagtat ccacccaatc ataaccagat gtaaagtttc
    31741 ctccaggata gcgaatgaca cctaattcta attcctttac ataagagagg acatcttgtc
    31801 taaacccgtc accatcagct gtttcatggc ccggttcata aatccctgta tagacgacac
    31861 ttcccatatg ctcaacaaat gagccaaaca gccgagaact gatctctcct accgtaaaat
    31921 cctctctgat ttttactttc acttcttcgt ccaacagctt tccctacctt tcatttccta
    31981 tagtcaagat aatagataca agtttccata cgccaccagc aaagataaaa ataaaaggag
    32041 ggaaaagaaa tatgagcagt acaaatccta ataacaatac gcaacaaaga atatttctca
    32101 aaaaatcaaa aaatactttc catgcacttt gttcaaaaat ttgtaaaaat gtgatatctt
    32161 tgttttcaga aatcacatat atccaggata aaagaaacat ggaataaata agtaaaacta
    32221 catatccact aattgaaagt acgattgtct gttcttctaa taattgatta acaagaaaat
    32281 aaataatgat cgttaacaga ctaaggaaca agttcttacc aaaatgttgc tttattcctt
    32341 gaaagaaaaa ataaatgata tttcctgatc cctcaccttt ccattgcgcc attacataaa
    32401 aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt acccagagaa
    32461 tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaaattta gtctcaacta
    32521 ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa aatatttctg
    32581 tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag ccattacagg
    32641 tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta aagttctcat
    32701 ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat tcatcagcgt
    32761 gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt tcaaaaaaat
    32821 attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg gtaaagtaat
    32881 aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa tgatcaaacc
    32941 tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa tcatgaagac
    33001 ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt ttcctttgaa
    33061 acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag tgatcactgt
    33121 ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg ttgtccaaac
    33181 cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa atgagataat
    33241 gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg caataaccaa
    33301 caatataact agcatactaa tcgttcgaat gattttttta ccactaactg cttttcgact
    33361 attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc tatttcattt
    33421 gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca acaatgataa
    33481 acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta aatcctcgac
    33541 tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg gttgcaacat
    33601 atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa aaacctaagg
    33661 agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt gcaccatcca
    33721 tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag agaatcgtca
    33781 tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata gcaagggttg
    33841 gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa ggaatcgccc
    33901 ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat gaacaaataa
    33961 ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta gataatgcta
    34021 ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg aaatataaag
    34081 aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa ttttcaaaac
    34141 ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata gcaaatgttc
    34201 ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt aaaaacaaat
    34261 acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag tccacctcat
    34321 ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag atatccgaac
    34381 aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc attcagatat
    34441 ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc agtaattgat
    34501 tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct aataattttt
    34561 caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa cctggtaaat
    34621 atggctctgc atagtccatt tgtttcgata attcagatac aataggatca ttttttactt
    34681 cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata ctccattttt
    34741 taccaatttc agttgtattc caatatttca aaaactcata gacagcttct tttttattat
    34801 tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt tttttgtctg
    34861 aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct ttcaatccgt
    34921 tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt aaattgtcca
    34981 tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt ttttggatcg
    35041 cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa tcatcgttca
    35101 ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca tccttattga
    35161 agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt tctgtcaatt
    35221 cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac aaatcattat
    35281 tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta ccttctactt
    35341 cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca tattcccaga
    35401 aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct ggataattta
    35461 atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt tcattcaaat
    35521 tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt ttattaaatt
    35581 cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc caaaaagtca
    35641 aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa gctcctaaaa
    35701 tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc attttttgtt
    35761 cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt caataaacaa
    35821 ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac ttgttcactt
    35881 ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc aatcactccc
    35941 tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc acggttaact
    36001 gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata cggcacttct
    36061 tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac ctctccttgt
    36121 ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc ttctccgttt
    36181 gtttctgtcc tgattggtgc aataacgttt accaattgag caagacaagc aatctttact
    36241 ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc atcttcaaaa
    36301 ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg ctcatcttgt
    36361 tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg cttcttacta
    36421 tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa ccgatccatg
    36481 tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata atattgatgc
    36541 agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc ccaatcacca
    36601 aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga atcatctacg
    36661 agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc tgttttatgt
    36721 ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat tccaaaaggg
    36781 ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg aaaattacaa
    36841 tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac agccatattg
    36901 atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac ttcattcgtt
    36961 tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg cccaatactg
    37021 tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat gggaatgttc
    37081 aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc tgatggatga
    37141 tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa tgaactgtac
    37201 aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt ggcttccata
    37261 actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt gtcaataata
    37321 taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt aataatgatt
    37381 aaaacaaata atttaatata acttttatta acaaatatgt taatatataa gaaaaacctt
    37441 ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg tatatgaagc
    37501 attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa agatgaatgt
    37561 caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc acgtgaaaaa
    37621 gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa tttcgagcct
    37681 tcgtgtcgat aaaatcgata ttagttttcc agaaaaaata ttcaatgctt ttgatacgaa
    37741 agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa catgcggttt
    37801 agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta tggatcctcg
    37861 aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc aggcacctaa
    37921 ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa tttcttctga
    37981 atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa atggtgtgga
    38041 gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat atacgcctga
    38101 ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa ttaccaaaca
    38161 cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca tccctaaaga
    38221 gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg tggggggctg
    38281 tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa aagtttacta
    38341 cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct atgaagggac
    38401 ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt ggcaaatgtc
    38461 gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga gcgggaactt
    38521 cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg acttcatctg
    38581 caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat aatttttccg
    38641 gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg aataatggca
    38701 aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt tctgcagcat
    38761 gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc gtaatcatac
    38821 gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct gcttcgctta
    38881 atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta acatcgggct
    38941 ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca taaggaagac
    39001 gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct tccatcgtta
    39061 gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct agtacaaaag
    39121 ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt ttacgtattt
    39181 cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct gtctgcaggc
    39241 tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt tcattcgttc
    39301 catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt atttttgcgg
    39361 attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg tatattcttt
    39421 gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga tatcttgtaa
    39481 aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca ctggatggaa
    39541 catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt agtctccttc
    39601 agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg gagtagttac
    39661 tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat catcttcgat
    39721 cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga tcaattttgt
    39781 cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa tcgttgcttc
    39841 atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat ctaattgttc
    39901 ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc cttggaagtt
    39961 gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg atttcaacgt
    40021 atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg ccataaatgc
    40081 ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc tagctggtat
    40141 cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga tatatgccgc
    40201 tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa attccaatag
    40261 tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca acgtttggaa
    40321 attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag gcccttccac
    40381 ttggatcaat ggttcatttg caaaaactag ttctccttca cgagcagaac gaactgttgc
    40441 tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt ctaatccacg
    40501 caagtaagca atatcggaat cattgaacgt cagattctct aaataattga ctaaccgttc
    40561 caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt aacattcaaa
    40621 aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat tgatttgata
    40681 tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct tgtattactc
    40741 ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag gaaaagaaat
    40801 cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa aatgaactcc
    40861 atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga atacttattt
    40921 tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta cttgataaaa
    40981 taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga acaaatataa
    41041 ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat acgtctttcc
    41101 ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga atttttttgg
    41161 aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca
    41221 cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa caaaaatggc
    41281 gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg agcaggttac
    41341 cataaagatg attattagtc gttattaaat agaatccttc gtattttttt gttctatcac
    41401 tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct cctgtttcac
    41461 tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc
    41521 gatttcttgc cttgtgattt tttcttcgta ttgatcatca cgacttgctt tttatcatat
    41581 gtgtctacca atagcagttg tgttcgatct aacgaaaata atttgtaata attaatagcc
    41641 gtattcccaa aaaagaattc cactacttta ggataaaatg tcttaatatt taaattacta
    41701 ttcacaggtg tttcgattag cttcatctca ttcctctttt ctatatagtc gatttagatc
    41761 caatcttaag ttatgttacc atctttccta gcattaagga atcggttaca attgaacctt
    41821 taaagacaaa aggatttacc ttttatcact taatagtata acataatttc atgagaaaaa
    41881 aatacaattt tttcttgacc atctcgttac gtaaaggatt atgcttacat ttgtcaggag
    41941 gaaaaataat ggagtacaca ataaagaaaa tgtcagagat atcaggagtc agtccgagaa
    42001 ccttgcgctt ttatgatgag attggactgc taaaaccagc taggatcaat tcttcaggtt
    42061 atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt tatcgaacaa
    42121 tggcattcaa gttggatgat atccaagagg tcttagacaa tccatctttt gaccaccaga
    42181 aagctttaat caagcatcag caaatgttgc tggaaaaacg ggcacaaatc gataccctct
    42241 tgacaactgt ccaacagaca cttgatatgt acgaaggagg aagaaaaatg agtgacaaag
    42301 aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag
    42361 agattcgaca aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata
    42421 tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca
    42481 ctctttactt aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc caagctcata
    42541 agaagtggtt atcttacagc tggcctgaat atcagccaaa agcgcataaa ggactaggaa
    42601 tgatgtacga agccgatgaa cggttcactg catattatga cgagcgaagt ggtcatggtg
    42661 cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta atcttttaca tgatccaaca
    42721 aaaaagtaca cgtaaaatga cctaataaca aaacagcaga cacccaagtt agatttttat
    42781 gctaactttt ggtgtctgct ccattctttc acaagttttt tcatcctact ttaaatatat
    42841 tcatcgttga ataaacgtat aaggtatttt cactttatcc tttttttctg taagaaacaa
    42901 ttgaaatgcc atttctccgc aagcttccag atggtggtcg atggttggga aaccaagaag
    42961 ctcgctgatc agcaaattat cgcgtccaat caccactggt gtcttatctt gcagatagct
    43021 ttgcaagatg cctgctgcaa ccatatccga atttgtaaag acaccatcaa ctccaagctc
    43081 tttaaaaaag aagcctgctt gttttccttt ctctgcatct atacaatccc aaaaaacaag
    43141 tgattcatca aaagatggaa aaagttgatg acatagctgc aaagtgattt ttgagttata
    43201 acttagcctg ccacttcgcc ccaacgtgat gcccaattgc tttatacctt gctgcttcaa
    43261 ataagtaaga ccttctgtaa ttgattgttc tcgatcaata aagacacagc ttgcctgttt
    43321 tcccttgatt tcttcacaaa aaactagagg tccatatttt tgatatgcca ataaatcatc
    43381 tagtgtattg gcacgagacg taatgatcaa tccatcgaaa gctttagctg caaattcttc
    43441 aagatacctt cgctcttttt ctttctgata gcccgtcggt aaaagtgtca ctttgtaatc
    43501 ataatcaaac gctgcttcaa tgatcccact gatcaactga tcaaaatacg gatgattggt
    43561 ataaggcaga atcactccaa tgcttttcgt ttttccataa cttaactgtc tggcatttgc
    43621 attagggata taatcgactt ctttcatgac cgcaagtatt tctttccttt tttcctcatc
    43681 tacatatgga tgtccattga ttacacgtga aatagtagaa actgaataac ctgtgagctt
    43741 tgcgatatct cgaatgtttg ccactgctgc ttcgccacct atacaaaaag attttcttgt
    43801 aaatagtcta ccacagctgg accgcattca aaaaactgat acatttttcc ttcatgcgaa
    43861 aaactgatcc gctgcccgcc ccaatatata tcttttgtca aattttcgat ttcattcatt
    43921 ggtatttctt ttactatatt cctattcaac ccttgaaaac tgtacaacgt taatacggat
    43981 cccttgaact cgacaaacgc ataaaaataa ttgttcgttg tgccatctgt tatctctaca
    44041 taagtattca ttgcaagccc tccttttgat actttgagta tagtgtatga aacgcgtctc
    44101 atagcaagga gtttgttttg ccaaagaaaa gttttattcc tctttcatta ttaccaaagt
    44161 ctcttatttt aaattgaata cattataatg atactgtaaa tatatcttt
//
LOCUS       DACCWK010000016        40288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCWK010000016 DACCWK010000000
VERSION     DACCWK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.016"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(34..2154)
                     /locus_tag="IVW65_000766"
     CDS             complement(34..2154)
                     /locus_tag="IVW65_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7899688.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="IVW65_000767"
     CDS             2384..3562
                     /locus_tag="IVW65_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7899689.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="IVW65_000768"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="IVW65_000768"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7899690.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="IVW65_000769"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="IVW65_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7899691.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="IVW65_000770"
     CDS             complement(4915..6168)
                     /locus_tag="IVW65_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7899692.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="IVW65_000771"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="IVW65_000771"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7899693.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="IVW65_000772"
     CDS             7746..9092
                     /locus_tag="IVW65_000772"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7899694.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="IVW65_000773"
     CDS             complement(9400..9786)
                     /locus_tag="IVW65_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7899695.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10665..10877
                     /locus_tag="IVW65_000774"
     CDS             10665..10877
                     /locus_tag="IVW65_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899696.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11155..12954)
                     /locus_tag="IVW65_000775"
     CDS             complement(11155..12954)
                     /locus_tag="IVW65_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7899697.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(13078..13674)
                     /locus_tag="IVW65_000776"
     CDS             complement(13078..13674)
                     /locus_tag="IVW65_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7899698.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            14024..14368
                     /locus_tag="IVW65_000777"
     CDS             14024..14368
                     /locus_tag="IVW65_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7899699.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            14369..14788
                     /locus_tag="IVW65_000778"
     CDS             14369..14788
                     /locus_tag="IVW65_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7899700.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(14880..15230)
                     /locus_tag="IVW65_000779"
     CDS             complement(14880..15230)
                     /locus_tag="IVW65_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899701.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(15196..16527)
                     /locus_tag="IVW65_000780"
     CDS             complement(15196..16527)
                     /locus_tag="IVW65_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7899702.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            16782..17348
                     /locus_tag="IVW65_000781"
     CDS             16782..17348
                     /locus_tag="IVW65_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7899703.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            17493..17580
                     /locus_tag="IVW65_000782"
     tRNA            17493..17580
                     /locus_tag="IVW65_000782"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17525..17527,aa:Ser,seq:cga)
     gene            17916..18890
                     /locus_tag="IVW65_000783"
     CDS             17916..18890
                     /locus_tag="IVW65_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7899704.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            19215..21110
                     /gene="asnB"
                     /locus_tag="IVW65_000784"
     CDS             19215..21110
                     /gene="asnB"
                     /locus_tag="IVW65_000784"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7899705.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(21305..21751)
                     /locus_tag="IVW65_000785"
     CDS             complement(21305..21751)
                     /locus_tag="IVW65_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7899706.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            21979..22128
                     /gene="rpmG"
                     /locus_tag="IVW65_000786"
     CDS             21979..22128
                     /gene="rpmG"
                     /locus_tag="IVW65_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7899707.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            22216..22734
                     /locus_tag="IVW65_000787"
     CDS             22216..22734
                     /locus_tag="IVW65_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7899708.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(22811..23767)
                     /locus_tag="IVW65_000788"
     CDS             complement(22811..23767)
                     /locus_tag="IVW65_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7899709.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            23933..24739
                     /locus_tag="IVW65_000789"
     CDS             23933..24739
                     /locus_tag="IVW65_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899710.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWTLDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            24736..25725
                     /locus_tag="IVW65_000790"
     CDS             24736..25725
                     /locus_tag="IVW65_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7899711.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            25722..26726
                     /locus_tag="IVW65_000791"
     CDS             25722..26726
                     /locus_tag="IVW65_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7899712.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            26858..27400
                     /locus_tag="IVW65_000792"
     CDS             26858..27400
                     /locus_tag="IVW65_000792"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7899713.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            27420..28118
                     /locus_tag="IVW65_000793"
     CDS             27420..28118
                     /locus_tag="IVW65_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7899714.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            28139..28351
                     /locus_tag="IVW65_000794"
     CDS             28139..28351
                     /locus_tag="IVW65_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7899715.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            28351..29313
                     /locus_tag="IVW65_000795"
     CDS             28351..29313
                     /locus_tag="IVW65_000795"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7899716.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            29331..29726
                     /locus_tag="IVW65_000796"
     CDS             29331..29726
                     /locus_tag="IVW65_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7899717.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            29889..30254
                     /locus_tag="IVW65_000797"
     CDS             29889..30254
                     /locus_tag="IVW65_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7899718.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            30251..32062
                     /locus_tag="IVW65_000798"
     CDS             30251..32062
                     /locus_tag="IVW65_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7899719.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(32124..32291)
                     /locus_tag="IVW65_000799"
     CDS             complement(32124..32291)
                     /locus_tag="IVW65_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7899720.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            32540..33529
                     /gene="galE"
                     /locus_tag="IVW65_000800"
     CDS             32540..33529
                     /gene="galE"
                     /locus_tag="IVW65_000800"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7899721.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            33541..35025
                     /gene="galT"
                     /locus_tag="IVW65_000801"
     CDS             33541..35025
                     /gene="galT"
                     /locus_tag="IVW65_000801"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7899722.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            35049..36029
                     /locus_tag="IVW65_000802"
     CDS             35049..36029
                     /locus_tag="IVW65_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7899723.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            36118..36942
                     /gene="tsaB"
                     /locus_tag="IVW65_000803"
     CDS             36118..36942
                     /gene="tsaB"
                     /locus_tag="IVW65_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7899724.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            36926..37468
                     /gene="rimI"
                     /locus_tag="IVW65_000804"
     CDS             36926..37468
                     /gene="rimI"
                     /locus_tag="IVW65_000804"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7899725.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            37475..37939
                     /gene="rimI"
                     /locus_tag="IVW65_000805"
     CDS             37475..37939
                     /gene="rimI"
                     /locus_tag="IVW65_000805"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7899726.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            37936..38952
                     /gene="tsaD"
                     /locus_tag="IVW65_000806"
     CDS             37936..38952
                     /gene="tsaD"
                     /locus_tag="IVW65_000806"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7899727.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(39524..40225)
                     /locus_tag="IVW65_000807"
     CDS             complement(39524..40225)
                     /locus_tag="IVW65_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7899728.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT        13118 a         7028 c         8219 g        11923 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactag tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cgtaggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gtttgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttgtctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gctggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcatatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgc ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaaccg ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggtgtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta ttatattaaa tattatacag ataataacgg
     9841 aatattatca tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga
     9901 acttgtgttt ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga
     9961 atacaaaaag acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga
    10021 tctcatcagt cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac
    10081 tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat
    10141 aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt
    10201 cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg
    10261 aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt
    10321 tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca
    10381 atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa
    10441 taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt
    10501 tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa
    10561 aataaaaaat aaaaaaatga ataaaaacgt tttcaaatac aaggtaattt gttattataa
    10621 aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag
    10681 taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg
    10741 aacgtacata tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc
    10801 aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcaccta
    10861 ctgaattggt ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa
    10921 taaagacaga agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat
    10981 tattcttgca gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga
    11041 atcaagagaa agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt
    11101 ggcaggttat gcgttttttg ttgtataaag acttttttca ttaacgattt ttgattagcc
    11161 gactcccaaa acaaaattta gcagtttatc tgaataagtg atttcttcat ctgtttgttt
    11221 cacactttca tttagtagtc ccatctcatc ggttatgata ccatccacac cataaaacat
    11281 catgcgactc atggcatctg aatcgttgat tgtccataca tatacttctt ttccgtcttg
    11341 gtgggcagaa ttaatgaagt tacggttgat cgtactatat tccattgtca gaaaatcagc
    11401 aggcgtcaca gggggcccaa caatattgaa aggtaagatg tagcccacat aaaagtttgg
    11461 ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt
    11521 ctctaagata actttgcggt atttatggac aaatcgttcg acaaggtcat ccgaatcttt
    11581 tttagaggtc ttgatctcaa taagtaattt ttggtttagc ttatgtgctt tttccaaata
    11641 agaatcaaaa gaacaaacag gggcttgcac tccgttttca gtaactgtaa gatttgtcaa
    11701 ttctttcaat gtaagatcct gtggtgttcc atttacccct gttaagtgac gaagatttgc
    11761 atcatgcatc actacgaaat gaccatcttt tgtttcctga acatccatct ctacataatc
    11821 aggatgatag tctctgcttg ttttttcaag tgcttctaaa gtattttgcg ccccattttg
    11881 ggtactcaca cctctgtgag agacagtcac tggttttgtc tgaacagctg aagtcagata
    11941 atccatattg taaagacaga cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt
    12001 gtttttcagc gcagagaaac gcaagttggg ggcttgtggg ataaaccact tagggatctc
    12061 aggcaagaag ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc ctacggtaga
    12121 catcacgata ttgaataata aaatcccttg taaaagcgtc atagcaaaca cagcgctgat
    12181 caatgcatag tctgggaaaa attgttcaac catactttgt gccaaaataa caacgatata
    12241 tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca ataaattggc ccgtgatggc
    12301 caaaagacgc gatttggtca atgaccagct ttcccgaatg gctgctcgaa aagaacgatc
    12361 tcgtagaatc atttccggta aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat
    12421 ataaacgagt aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa aatccataat
    12481 gaaagcgggg atttttattt ttgacagtag atcagaatta aaacttaatc cgctgatcgg
    12541 tagaatcaat aaaaaataag caaggaaaaa caaaaaagta atgggtctca ctttcttcag
    12601 ctgaagtatc gtcaagtgta agagttgctt caaagagatt ggttctttct ttttaataaa
    12661 aaaaacactc atcaaaagaa aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa
    12721 aactacgatt aacgagagta gaaccccagg atgttgagaa aaaattgaag gaatattgtc
    12781 ataagacaaa tagtcaattg cccctcggtg taaaataaaa cgggtcgtac tgctcaataa
    12841 gggtaaacaa ataaataaaa taaagccatg catcagcaag acatcacgga aataagcttg
    12901 tgttcctttt aaaaagtccc aagcattacc taagctacgt ttcaaataat tcatcctatc
    12961 acctgctctt cacattgtag cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg
    13021 cagacccttt cccagattta acattgtcaa tcaatgacta attttacaaa ataaatatta
    13081 tccaagaaga ccacttagat attgacctcc ataaatcaag gccatactat ataagaaaat
    13141 cgacagtggt ttagcaatca aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa
    13201 tccagccatc aaacataacg catcgtcagg agcaactgga agaaaaatag ctagagcaaa
    13261 cagcttctca aaacggtttt gattatccag ccagccaata tatttgttat atgtcttatc
    13321 gctaatcatg gaaagtatga atggctttcc ataacgacgc cctagcagaa aaatgataat
    13381 cgaaccaatg cagattccaa tatagttata gataaatcca gcataagggc caaatatcag
    13441 gacaccagct gcagtactga tccctccagg aatgattgga ataactactt gcaggatttg
    13501 gatgaagaca aaaataatcg gtcctaaaat gatggaatca cctactaatc ctcttaacgc
    13561 attcaaatct ttgaaaacac ctaaattaat aaaataaatc gtcagcgcga ttgataggcc
    13621 taatccgata atggatatga aatttatcag ttttcgagag gcagcaatat tcattattca
    13681 tcaactcctt agaggttctt tgttaactat actttactag aaaagcaact aactgcgcca
    13741 ttttttgata tgacagaaaa caaacaattt tgtaagttca tcataagtat aaaagattga
    13801 tctcaaaaaa acatccaact aaagacttat ttaataactt gtttttttta aaataaaaaa
    13861 agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat tggacaggct
    13921 gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt gccttagaac
    13981 catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac
    14041 cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga atgatgatgt
    14101 tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta gtatcatcac
    14161 catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc tgaagattgg
    14221 caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt tagcttttta
    14281 tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct atttagaaga
    14341 aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa tacacaatgg
    14401 cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta ccgggcaaag
    14461 ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta atgtaatttt
    14521 tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa tcttacttgt
    14581 gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat taatggctgg
    14641 agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa aagacagttg
    14701 gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg taacagatga
    14761 agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg aaagtagatt
    14821 ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag aaaatagggt
    14881 taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg ctgggcatac
    14941 atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg atcatgtcgg
    15001 ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg attgttcagc
    15061 aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg tatctctatg
    15121 aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc atgggaatat
    15181 aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa gtgtcttttt
    15241 ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg caacgaaacc
    15301 agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga cgttatggac
    15361 aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa aaccgcttat
    15421 gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg tgatctcatg
    15481 aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac tgttttttaa
    15541 atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga caggtccatt
    15601 taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta agcctatgtt
    15661 gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt ttccagatga
    15721 atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac caattccgac
    15781 tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt cattgaaaac
    15841 caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa aagtcatcgc
    15901 aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga aaactaaagt
    15961 ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat tccccaataa
    16021 aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga ccggtgataa
    16081 tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga caaatacagc
    16141 gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc caaaaaaaga
    16201 gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg
    16261 ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa
    16321 accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa
    16381 tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa aaaaaacacc
    16441 tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt gaggatgaat
    16501 ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc tttaactata
    16561 acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat gtaagggaac
    16621 ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa
    16681 aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa
    16741 taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga aaagtctata
    16801 ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg tttaaaggat
    16861 ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt tatctggaat
    16921 tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa caactaaaag
    16981 aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt ttgaactata
    17041 tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat gaacatgggg
    17101 acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt aaagaagatg
    17161 aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt acatgggtag
    17221 cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca gaagaacaaa
    17281 tcattgcact gatcaaacgg acaattgact cgattttagc attagataat cctgaaataa
    17341 tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc aaactgtcaa
    17401 tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat aggcttgaca
    17461 tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg taatgcaccg
    17521 gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct
    17581 tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa gaacggccaa
    17641 acaaaaatag cttttcctat tttttcgttt ggttgggctt attaccgcag atgcaagctt
    17701 ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca gccccttatt
    17761 gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt ttataaatgc
    17821 tagtaaatca atcttctttg gccttttttt tgataaaaaa aaatactaga tgtaagataa
    17881 aagtgtaaaa acgatttgtt ttaggaggga aaacgatgtg taccgcaatc acttatgcaa
    17941 cgaaagacca ttattttgga aggaattttg attatgagat gtcttataat gaagtagtca
    18001 ctattactcc aagaaattat cgtttcgatt ttagaaaggt aaaaaattta gataaacatt
    18061 atgcaatgat aggaatagca gctggggtat ccaactatcc actctattat gaggctacaa
    18121 atgaaaaagg gttgagtatg gcaggactga attttcctgg caatgctgat tataaagaat
    18181 tacaagaagg aaaagataac gttgcaccat ttgagtttat tccatggata ctagggcaat
    18241 gctcaaccat agacgaagct aaagaattat tggctactat caatctagtc aatatcgatt
    18301 ttagtgaaaa actacccttg tcacctttac actggttatt agctgataaa gaaaaatcga
    18361 tagtaattga aagtatgaaa gatggacttc atttatatga taatcctgtc ggtgtgttga
    18421 caaataatcc gccatttgat tatcaattat ttaatctgaa caattatcgc tcgctatcaa
    18481 atggaacacc agaaaatcat ttttcaaacc agatcagctt gaatgtttat agtcgtggga
    18541 tgggaggatt gggcttacct ggagatctct cttctgtttc tcgatttgta aaagccacat
    18601 ttactaaaat gaatgctgca tcaggtgatt cagaatctga aagtatcagt caattttttc
    18661 atatactagg ctcagtcgag caacaaaaag gagtatgcga tactggagaa ggaaaatacg
    18721 aatatacgat ttattcttct tgctgtaacg ttgataaagg gatttattat tatcgtacgt
    18781 atgaagacag tcaaattact gctgtaaata taaataaaga agacttggat cgtcatgaat
    18841 tggtcagtta tccagtcata aaagagcaac agataaacta tgtcaattaa ttaatgaata
    18901 gatccgtttg gtatcatatt ttctgataca gtcataatta gacacgatct ttaggtcgtg
    18961 tctttttttg ctgttttttt tggaaatagc atccactaat atttttttaa cacgaagaga
    19021 ataaggtagc ctcaaaaaag aaaacatggt aaattagata ttgggacttt attttctgta
    19081 aaaacagaaa ctttcatctt ttgttttcac gtgacgaaat tgtaagagag aaaatattcg
    19141 tccgctattt attctatgat aagatgcata tgaatgaaat aaacagtaat tttatattag
    19201 gatgtgaaag ttaaatgtgc ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa
    19261 caattattaa tacaatgatg gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat
    19321 atatcgataa acatgttgct ttaggattca gaagattaag tattattgac ttagagggcg
    19381 gtactcagcc tatttacaat gaagatcgga caaagatcat tatttttaat ggcgaaatct
    19441 ataattacca gcctttgaga gaagaattga ttgcagctgg ccacgttttt caaacacatg
    19501 cagatacaga agtccttttg catggttacg aagaatgggg aacagaatta ttgcaaaaga
    19561 tccgcggaat gtttgctttt gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa
    19621 gagaccactt tgggatcaaa ccatattatt atgcagaaat gaacggaaca tttatgtttg
    19681 gttcagaaat caaaagtttt ctgcctcatc ctgattttaa caaagaattg aacaaagaag
    19741 cgttgaaacc ttatatgact ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag
    19801 atgtctatcg tttgcctgaa ggccattatt atacgtataa agatggaaaa ctagacattc
    19861 agcaatattg ggatgcagat tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg
    19921 aaaaaatcga cgaaaccgtt caagcttcta ttgaagcaca tacggtcagt gatgtagaag
    19981 ttggttcttt cttatccagc ggggttgatt ccagttatgt aacctctgta ttgaaaccag
    20041 atcattcttt ctcaatcggg tttgatgata aaacctataa tgaagcgatt gaagccagaa
    20101 aactaaccga actattggac ttagataata cagcagcagt gatcgacgga gacatgtcgt
    20161 tcaaagcatt ccctttgatc cagtatcact tagatgaacc tgattcaaat ccttcttgcg
    20221 tacctttgta ctttttagca aatcttgctt cacaaagtgt gcgggtagtc caatctggag
    20281 aaggggcgga tgaactgttt gcgggatacc aaacgtatgg attccatacg aattcaaaat
    20341 tcatccgtgt gattgctcaa ggattgaaaa aattaccaaa aggaacacgc tataatttag
    20401 gacgtaaaat cggaaaaatg aaaaatttcc atggacggat ccatttatat gaatcacttg
    20461 ctcctgcaaa agaatatttt atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag
    20521 aagtgttaac accaaaatat caaacagcac catctgtgga tgaaatcatg actgttcatt
    20581 acgagaaaac agaaggaatc aaagatgaag tcaataagat gcaatatgtc gatcttcatc
    20641 aatggatgcc aaaagatatc ttgttaaagg cagataaact ttctatggca agttctttgg
    20701 aagtccgagt acctttattg gatatcgaag tcatgaaatt ggctcaacaa ataccaagta
    20761 agtttttatt gaatcaaaac aacacaaaag atattttcag acaagctgcg aacaaacatc
    20821 ttccggaaga atggtcgaat cgtgtgaaat taggattccc tgtgccaatc aaagcttggt
    20881 tgaaagaaga acacggctac gaacaagtca aagcattatt cgaagcagat tttgcaaaag
    20941 aattttttga tcaagaaaaa atcatgaagt tgctagatga ccatcacgaa ggacgtaaag
    21001 aagagcaaag aaaaatctgg acaatcttca gtttcttgac atggtataaa gtctactttg
    21061 tagatgaaac gattccgcaa gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa
    21121 atatcaataa aaaagaggtt ttctcctacc gtttaacact gagtgataag aaacaatttc
    21181 aaaaaacagc tcttcccgtt ttatagatat tatgacttat ggttcggagt tggcagagga
    21241 acaccattgt ttcgaacata agggggttgt gacacgattt tcgtcacaac ccctttacat
    21301 atgttcaata atcgatttct ttttcttttg tcccatcttt tttataaatg aacaaagttg
    21361 attctttgtt ttgtgctact tgctttcctt tttcgattgc ttcttttttt gtatcaaaat
    21421 ggttgcttgc cttttttgcc cctttagatt tcacgatcca ttgttcttcc tcatattcta
    21481 cgatcacatc tgaatcaagc agttttccag cacgaggatt cgtatcgtgt tcatccgtct
    21541 tgcttggatt tttttctttt tcgaaagttc gtttttctga ttcactagca tcttcatacc
    21601 atttttttgc ttgatcaatg gcaattggga tcgcacggtt atcagggtac ccttcatcta
    21661 ataatgcatt tgcgatgtcg attgcttttt ttcttgtcaa tttatctaag tttttcattg
    21721 atgcaggaaa gtccttcatg ttccatggca tattagtatc cccctttatt aaaaaacata
    21781 atctatataa acaagtgtaa gggtatttat acaaaaagaa aaagattttg tttgagaagt
    21841 aaaaccgttg tatttatcgt caagtagttt tacttaacaa aatcgtgaaa aacactagca
    21901 atctagaaaa aatcatggta agataatcaa gtctgagagt catcttagag gattcttgta
    21961 aaaatgggag ggaaaaacat gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa
    22021 cgtaactatc taacaagcaa aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa
    22081 tattgcccac gcgaacgcaa agttactttg caccgtgaaa caaaataatg tttttaaagt
    22141 tctcaattcc ttgctggtca aagaattgag acttttttta tctcaaaaca tctaaaatca
    22201 taaggagcgg ataatatgtt tatttccatg tgggcacaag ataaaaatgg tctgatcggc
    22261 aaaaatggtt tgttaccatg gcgattgcca aatgatatgc gtttctttcg cgaacataca
    22321 atggatcgaa ttttagtgat gggaagaaag acctatgaag gaatggggga tctttcactt
    22381 ccttatcgcc atatcatcgt gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg
    22441 gaagtaatgc attcgatcga tgaacttttg tcgtatagtc aaacgatctc agaagatatc
    22501 tatgtttcgg gtggtagtcg gatttttcaa gaacttcttc cttacactgg tcggatctgg
    22561 cgaacattga tcgatagcac gtttgaaggc gatacgtata ttgggaatat tgatttttca
    22621 gacttcgccc ttatagagga gtatgagggg ataacggatc aagaaaatct atatgcccat
    22681 cgttttcaaa aatgggaaag agtaaaaaat agggttgctt caaaagaaga ataaaatcta
    22741 tgcgtctagt ttgttaataa gaaaaaaagc atctcacgac taatcgtgaa gatgcttttt
    22801 ttagttaaaa tcaatttagt ttttctttga tatcattgac catttcctga ctggcaatag
    22861 gtccgttata tagccaacta gattcaggtc cgaattgcag tacatgattg tctgtcactg
    22921 ctttaagatt cttccagctt ggttcattga acatggcagc atctttgtca ctgttcacaa
    22981 ggatgatgta gtcagcatct aattcagcta gcttttcaga ggaaacagca gaccagtcag
    23041 aagtggcttt tttggagacc tcttccacta gatcaggaac accaaattcc agatcgtcgt
    23101 atacgattct tccacttgac cgatttttgc tgaccataaa ggctgagttg ttggtcaccc
    23161 acaacactgc agctgtcttg ttctcgattt tatctgctag ttcttctcga gttttcttga
    23221 tggatttttg ataatcggaa atgatttttt ctgcctcttt ttcttttgcc aaagctttgc
    23281 cgacatcttt tagttgttct tcccatgtga catctgttcc gttttttaca acataagttg
    23341 gcgcgatttt gctatattgt tcgtatttcc cgccttcgac agtagcggaa gaagaaatca
    23401 acagcaaatc aggctcaaag cttaacactt tctcataagg aaggtcataa gaaatcgttg
    23461 ggacatcctt taaatcatct tgcagataat cttgaatgga accactgcca acagtccact
    23521 gggcgatagg tttttcccct agtgcaatca gatagtcttc taagtaactg ccgatgatcc
    23581 gtttgggcgc gtttggaatc tctactttat gtccaagtgt atctgtgact gtatgagtac
    23641 tattggatga agtcgtcgaa ttggcagttg tagctgtttc tttatctgaa gagggggagc
    23701 aagcgctcag tactccagcc agcagcaata tgctagataa tgtgaatact tttgttttta
    23761 atttcatttc tttcctccta acataattga gaatctttct cattggttat tattcctgat
    23821 atttcgaacg ctgtcaaccg cttcatttgg ctatagatat gaaagtagcg cttgacgaaa
    23881 tgttgaaaca gcgtcataat tgagaatgat tttcagcgaa aggatgggtg aaatgacgag
    23941 attaagagca gctgacattg cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc
    24001 tgttacgatt cctgaaggga aaatcactag tttgattggg ccaaatggaa gtggaaaatc
    24061 tactttattg aaaagtttta cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa
    24121 taactctgat atctctacat acagttcaaa agaacttgca aaaaaaatta cattattagc
    24181 acaaactagt gaacacccta caggtatgac agtagaagaa gtagtatctt acggacgata
    24241 cccttatcaa aaactgttca gtggcatgga cacgcaagat catgaagcaa tcgagtggac
    24301 gttggatgct acaggtctag ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg
    24361 acaaacccag cgtgtctgga tcgctatggc gcttgctcag gaaacagata tcttgatctt
    24421 ggatgaaccg acaacatttt tagatccggc acaccaatta gagatcctac agttattgac
    24481 tgaaatcaat caaatgaaac aaacaacgat cttgatgtcg attcatgaca ttaatcatgc
    24541 ttcacgtttt tcagattatt tattagcaat gaaaaacggt aagttgatca cagaaggtac
    24601 acctgaagaa gtgatcacaa aagagtgtat gaaaaaaata tttgagattg atgcaacgat
    24661 tgtcacgctt ccaagaacaa ataaaccagt ttttctaacc tatgatttgt tgccaaaaga
    24721 gcaggaggat caattatgag gactatgaag aaaaatagac tcctgctttt ggtctgtttt
    24781 gttcttttga ttattttttt tgttgctctt tcaaatggcg cagcgactgt gggaaaagca
    24841 gatttttggc aggcgttgtt tgattttgat agatcaaaac aaagtcacca aatcatacga
    24901 aatattcgtt tgcctcgtgt ggcgggagca tttcttgtag ggagttgttt tgcattgagc
    24961 ggtgcattga tgcaaggagt gacaagaaat cctttagcag attcaggctt actaggaatc
    25021 aacgcgggtg cttcgttggc aatggccctt agttttgctt tttggcctaa agcttctgat
    25081 ttttctgtgt ttctaatgtc attggcaggt tcttttgctg taactttgct cgtatttggt
    25141 tttttgcagt tttcgaaaat gggcatgaat cctttacagc tgattttagc tggcgtagct
    25201 attagttctt tttttactgc tatcagccaa gcacttaccc aattctttaa tctgcagcag
    25261 gatcttgctt tttggtttgt aggcggagct gccaatatca catggaaaca attagcagta
    25321 ttgtttcctt tatatatact tgctgttttg gtttcttttt cattaggaaa agcaatatct
    25381 ttgattcatt tgagtgaaac acatgcatta gctcttggaa gtcatcctaa acgaacgcgg
    25441 ttgattgttt tctgtctagt tgcagtgtta gcagccattg ccgtttcgtt agtcggacct
    25501 gtttcattta ttgggctgtt ggttccgcca gttgttcgcg gaatggttgg aacggactat
    25561 cgttatgttc tacctgtctc ttttttagtt ggcggtggat tggttatgtt ggcggatttt
    25621 acagcgagga tgatcaatcc cccttttgaa acaccttttg gattagtcat tgcattgatt
    25681 ggcgttccgt ttttactgat acaagtaagg agggaacaac ggtgaaaaaa atatattgga
    25741 cgttctattt cttgcttttt gtacttgtca tcctgtctat aggtagttta atgattggta
    25801 cgccttttat ttacctggat cagctttttg ccacgttcat agggaaagaa tcatcttctt
    25861 tggcgctgat tgttagagaa tttcgagttc ctcgattact gatcagttta ttagcaggag
    25921 cttgtcttgg cgtttctggt tatatcttgc aaggagtaac aagaaatgaa ttggcagatt
    25981 ctagtatatt gggcattaat gccggagcag gatttttagt gatgctttat ttaggtttct
    26041 tttcgcacta ttcgctgccg tttctattgc tagttgtagc ctttctaggt ggtgttttag
    26101 ctgcttgctg tgtctatctt gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa
    26161 tcttattgtc cggtattgcc gttaatgccg gcctttctgc attaacattg ctatgcacga
    26221 tccaattatc taaggaaaat tatggatttg tgaatgcatg gttagcggga tcaatctggg
    26281 gtgcgagctg gccttatgtt tgggcattgc ttccatgggc ggttatctta attccgctag
    26341 gaggacttta tgcacagagg attggattgc tatcatttgg tcaagaacgc gcgcaaggat
    26401 taggccttaa cgtggctaaa agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa
    26461 gcggtagtgt agcggtgaca ggtagtttat cttttgtagg actactagct cctcatgctg
    26521 cgaagctagt cgtacgtaaa gaaaataact ggacctttgt tctgagcggt ttgttcggtt
    26581 ccatttttct gcttagtgca gatatggttg ctagagtgat tctaccaagt ggggaagtac
    26641 ctacaggaat tttgattgcc ctttttggtg cgccatattt tctgtatgca ctttttatca
    26701 gacaaaaaca cgcgctgaac agttgaaatt tattaaaaaa ggaaggccaa tcatttctag
    26761 gtcttctttt tattctattt atgcatttat ttgaaagttc gattgctatt tttctttttt
    26821 tcctatacca ttttatgaga gaaggaaggg tgagtttttg gaccaattga aagtaagatt
    26881 acgccggaaa ggaatcgaaa agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa
    26941 agaacagcga atctataaac agctattcgc cagcgagtac tggaagtcag caaaagtgat
    27001 agggacgact ttatcgaatg aatttgaact ggatacacag ccggttatcc aacgagcaga
    27061 aaaagaacaa aagaaaatag ttgtacctaa aactttgccg aaacgtcaga tggcgtttta
    27121 tgaaatggat gaacagtccg ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc
    27181 agagaagctc tatttgccgg agatgatcga tctcctcatt gtaccaggta ttatctacaa
    27241 tagagccggt tatcgaatcg gttttggcgg tggttattac gaccggtatt taaataattt
    27301 tcataaaaaa acatgtagcc ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga
    27361 gagctttgat cagaagatcc aacaattgtt tattgactaa aatgaaatgg gaggttttca
    27421 tgaattatca acagcaaata aaattgcgtg catggttaag acgaccgtta tggacctatg
    27481 cttttttagg gatacagaca gcggtcttcc tcatgatgga attatttccg cgacttgaga
    27541 tcccttatta tgccggaatg tatggtcctt atcttgttca cttcaatgaa tggtggcgtt
    27601 tagtgacacc aatcttcatc cattttggac tgatgcattt cgtaatgaac tcactgatac
    27661 tgtattttat ggggcagcaa atcgaggcaa tctatggcca ttggcgtttt ttcttgattt
    27721 atatgttcag cgggataatg ggaaatacag caagctttgc ttttaacgaa gcgaatgttc
    27781 tgtctggtgg tgcgagtact tcgatattcg gtttgttcgg tgcgttgttt atcttagggt
    27841 ttcattttaa acataaccca gcgatccagc agctagtcag acattttctt ttatttattg
    27901 tgttgacatt tgtttttggt cttctggata cctctgtaga tgtttgggga catgtcggtg
    27961 ggctaattgg cggattagtg ttagggaatg ttttgggtct accgaaacaa cagacaagct
    28021 attcgataca ccaaaggatt ctttctacac tagtttttgt atttcttttt gtaatatgta
    28081 ttttactggg tttaaaaaaa tatggtttac ttgtataata tgaaggaagt gcttaaagat
    28141 ggagagctta tatgatgtac agcagctgtt caaaaaattt ggcatcttta tctatgtggg
    28201 tgcacgcatc tatgacattg aattaatgac tatcgaattg cgtaatctgt atgatagccg
    28261 tttgattgat catgatacat acatgacagc gtggcgcata ttaaaaagag agcaccgaat
    28321 tgaagaaagc cgaaagaaag gaaatttgta atggacaaga agattattgg gattgattta
    28381 ggtggaacaa cagcaaaatt tgcaatttta acaccagaag gagagattca acaaaaatgg
    28441 agcatcgata ccaatatttt ggatgatggt aagcatatcg ttcctgaaat cattgaatca
    28501 atcaaccatc gtttgaattt atataatatg aaagccgaag actttatcgg aatcggcatg
    28561 ggtaccccag gaagcgtgga cagcgaagct ggaacggtta ttggtgcgta taacttaaac
    28621 tggacagaag ttcaatttgt gaaaaaactg atcgaggcag gaacaggaat caaattcgct
    28681 atcgataatg atgcgaatgt tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac
    28741 gatccagacg ttgtattcgt tactctagga acaggtgttg gcggtggtat cgttgctggc
    28801 ggaaatttga ttcacggcgt gagcggagca ggcggagaga tcggccatat cactgtggat
    28861 cctgaagggt tcgaatgtac ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct
    28921 actggtgtgg tacgtctagg tcgttacttg gcagaagaat acgcaggaga ttcaaaactg
    28981 aaagcaatgt tagacaacgg tgaagaagta acaagtaaag atatctttga aatggctcaa
    29041 gaagacgatc cgtttgcatt gatggttgtt gatcgcgtat gcttctactt aggtttagct
    29101 tgcgccaact tggggaatac attgaatccg tcaagtattg tgttaggtgg aggagtatcg
    29161 gcagcaggag aatttttacg tagccgtgta gagaaatatt ttaaagaatt tactttcccg
    29221 caagtaagag aatcaacaaa aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt
    29281 ggtgctgcct cattagcctt gaaatttgct taaaaaaacg gaggcattac atggttttat
    29341 gggtaattaa tatcattttg ctattgattg tgcttgcaat catcttttgg gaagtctatc
    29401 tacgagtaat ggccaagcgt tcagcaacga ctttgacaga agaagaattt cgagaaggaa
    29461 tgagaaaagc acaagtaatc gatgttcgcg aaaaagatgt gtttgatgct gggcatattt
    29521 taggtgcaag aaacatccct tacactgttt tgaaagattc attaggatct attcgaaaag
    29581 accagcctgt ctatatttac gatcaaaaga aaagcttgag catccgtaca gcaaataaat
    29641 tacgtaaagc aggttatcaa gagatttatt tacttaaggg tggatatgac ggatggtctg
    29701 gaaaaatcaa aagtaaaaaa gtataaaaca gatgattgta tgacagcttt aaaaaccgaa
    29761 tcaatcatta gattttgacc aagatcacat gaacggcgca atggatagtt gtgccgttca
    29821 ttttttttat tttgtgctat tatctacata tataagctaa gcgaaaaggc agaaagaagg
    29881 aaacaactat gatcaaatta aaacgtgttt accttgatgc tgaaaaagag gatggctata
    29941 gaatactcgt ggataggttc tggccacgag gaatttcaaa agaaaaagcg cagttagatc
    30001 tgtggttgaa agaaattgca cctagtaatg aagcacgcaa aacttttgac catgtaccag
    30061 aacgatttgc ttcatttgca aagaaatacc agcaagaatt gattttcgaa gaagaaaaga
    30121 agcaagctat ttttaaactc agtcaactta tgaaagaaca tccagtcatt acttttgttt
    30181 atggtgcaaa aaatgaacat cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg
    30241 aggcatgcaa atgaaaatag ggatcattgg tgccggacct agaggattaa gtatagcaga
    30301 aaggattctc cataataatc aggacagaca ttctcttgaa ctttttcttt tcgatcccaa
    30361 tggtcctgga ggtcgagttt ggcggttgaa tcagccaccg gagttattaa tgaattctgt
    30421 ttctcaacaa gtgacgcttt ttacagatga aacattgtcc agtggaggat tggtaacaca
    30481 gggacccaac ttgtttcaat ggagtaaagg acctgctaca gattatatcg aacagacggt
    30541 aaccaaaaat aagcaactaa ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc
    30601 tactcgatgt ttatacggac tctatcaacg ctggtttttc tcaaagttgc aagaagaatt
    30661 tccagcttct attcagtttg tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg
    30721 ttatctttta cagacagatc agcaaagcta ttctatcgat cagcttgttt tagcaagcgg
    30781 tcactgggaa aatgatgaga cagaagaaga aagagtgtgg gtagactacg caaagaaaaa
    30841 tgatttactg tatcagcttc cttctaatcc tgcagatgtc gatctttcga agataccgcc
    30901 taaagaaaat gtctttttaa gaggtctagg cttaagtttt tttgattatg tcggattgtt
    30961 tacagtatca agagaagggc gcttcgagaa cgaaaaagga aaacttgtct atcatccatc
    31021 aggcaaggaa ccgattgtat acagtggatc aagaaggggg ctcccttatt accctcgagg
    31081 aagaaaccaa aagcaaggcg gatcaatggc atggccacgg ttactaacaa aagaaaacct
    31141 ggagcaatgg catcagtcgg gatttttgac tggcgaaaca ttttttgact atctgaaaaa
    31201 agacgttgag ttgttttatt acaagaaaat catcgaagaa tatcatttgc cgattccaaa
    31261 gaaaaatttt gagcatgatt ttctctccct ctcttcagag gagtgtctcg gaaagcatcc
    31321 agcactaatc ccttatagat gggattggtc gtatttagaa attcctctta cctatcagga
    31381 tgagtccttt gcagaagcta gccgagcatt tattgaaggg cagataaaag aagcggaaaa
    31441 aggcaattgc acaggagcat ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc
    31501 aatccggcag gctattgaat ggcacgtttt ttctgcaaag gaatacaaag aaatactatg
    31561 gggatggttt actcatctaa atagcttttt aacgatcggt ccgccagtca ttcggacgaa
    31621 agaattagca gcactgatag atgcgggtat ttttcatttg ttcgaaccgc catttgatat
    31681 tcagcaaaag cacaaccgct atgttatttc tctacaagat aaagaaatca gcagtcgcta
    31741 tttgattgaa gctcgattgc cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa
    31801 aaatttgaag gaaaacaaac ttgttcgccc tttttcattt tcagataata caggaagtta
    31861 tcattcagaa gctatagatg ttgagcgagg tacgaatcgc gtgattgatg cttcagggga
    31921 aatccagtct gacttttatt gctatggaat acctgttgaa ggtgtggatt ggctaactgc
    31981 agcagtagct agaccttata cagatccatg gaatctgaga caggcagata aaatagcaca
    32041 acttatattg actgacaaat aaaaatacgc atgatgactt atctgttctt tcacaagtct
    32101 tcatgcgtgt ttttaaataa ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg
    32161 ttttcttcga tcattgcttc tttttcgtca attggatcga tcactgtttt tttcacagaa
    32221 gcgaccaaac cagcaacggc agcggctgtt gccagacttc ctactaatat tccagataca
    32281 aattttttca ttgaatccac ctcatttatt atttgttata tctattatgg aacaaaagag
    32341 aaaaaaaggc tagttttacg tttgtttgta aacctttgca aaaaatcctt gaaaatgttt
    32401 actaaatgtt ttattataaa tttaccatat gaatttacga aattttactt tgtttgtttt
    32461 caccataaaa gtaaacttcg tttttttaaa ttatatataa tcaacaaaca caattttact
    32521 aatcaaagga gtgtttttta tgacaatttt agtactaggc ggagccggtt atatcggttc
    32581 gcacgctgta gaccagctag ttcaaaaggg gtatcaagta gctgtggtag ataatttgct
    32641 cacaggtcac aaacaagcag tacatccaga tgctcatttt tatgaaggag acatccggga
    32701 taaagaattt ctacgttccg tatttgaaaa ggaaccgatt gaaggagtaa tccattttgc
    32761 tgcaagctct ttagtaggag aatctgtcga aaaaccattg atgtatttca acaacaacgt
    32821 atacggtatg caaatcttac ttgaagtcat gcacgagttc aatgtgaata aaatcgtgtt
    32881 ctcttctact gcagcaactt acggagaacc aaaagagtct ccaatcacag aagatacacc
    32941 agctaatcct aaaaatccgt atggtgaaag taaattgatg atggaaaaaa tgatgaagtg
    33001 gtgtgaccaa gcatatggca tgcgctatgt cgctttgcgc tacttcaatg tagccggagc
    33061 aaaagcagat gcatctatcg gagaagatca tacaccagaa acgcatcttg tcccaatcat
    33121 tttacaagta gctcttggtc aaagaaaagc attggctgtt tatggagacg attatgatac
    33181 accagatgga acatgtatca gagattatgt tcaagtagaa gatttgattg ctgcacatat
    33241 tttagctttg gaatacttga aagaaggaaa tgaaagcaac ttcttcaatt taggcagcag
    33301 caaaggatac tcagtcaagg aaatgctaga agcagctaga gaagtgacag gaaaagaaat
    33361 tccagcagaa atagcaccaa gaagagctgg agatcctagc cgattagtcg cttcaagtga
    33421 aaaagcgaga gaggttctgg gttggaaacc tgaatacaca gatatcaaag caattatcaa
    33481 aacagcatgg gattggcatg tcagccatcc aaatggttat gaagaatagg ggaatgagat
    33541 atgtcaatca gtcaaacgat ttgtgatttt gccacacttg cgatcgaagc tggcggatgg
    33601 atggaaatgg accgcttata tttacagaat cgtcttttag ggatggtagg agaagatgct
    33661 ctagaaagca ttgaagtaag accagttaag gaagaatctg tagacttatt ggatcagctt
    33721 gtcgctaagg caagagaaaa tggtgttctt tcagaattgc cagcagaaca agagattttt
    33781 gaagcgcaat taatggattt tctcacaccg ccaccttcag tcgtcaatgc attctttgca
    33841 cagcactatt cgaaaagccc agaggaagca actgagtatt tctttaattt aagtaagaaa
    33901 aatgactaca tcaaaacaag agccatcgca aaaaacatca aatttccggc agagtcggag
    33961 tatggtgaac tggaaatcac aatcaattta tctaagccag aaaaagatcc aaaacaaatc
    34021 gcagcagaac gcgaggcggt aaaagtagac tatcctacct gcatgctttg catggaaaac
    34081 gaaggatata gaggtcgctt gaactatcca gcccgcacca atcaccggat tattcgaatg
    34141 aatctagacg gtgaaagctg gggattccaa tattctcctt atgcttatta caatgaacat
    34201 tcgatcattt tatcagaaca acaccgcccg atgaaaatca caaaggaaac atttgctaga
    34261 ctgcttcgga ttactgaagt cttgcctcat tactttgttg gttcgaatgc tgacttgccg
    34321 attgttggag gatcgattct ttctcatgat cattatcagg caggacgtca tgaatttcct
    34381 atggcaaaag caccgataga aaaacttatc aaactagcag attatccgca agtcatcgca
    34441 ggagtcgtga aatggccgat gtctgtgatc cgtttgcaat caacagagaa aaaattgttg
    34501 attgaagcag ctaactcagt attggaaaag tggaaagcat attcggatga aacagtatct
    34561 atcagagctt tttcagcaga tggtacacca caccatacga ttacgccaat tgcacgaaga
    34621 agggacaatt actttgagtt ggaccttgtc cttcgagaca acaatgtttc agaagaacat
    34681 cctgatggga tttttcatcc acataaagat gtccaacata tcaaaaagga gaacattggg
    34741 ttgatcgaag taatggggct tgctgtttta ccacctcgtt tgaaaccaga actagtagaa
    34801 gtagagcatt tcttattagg agaagaaaac cagatggctg aatatcatcg tccgtgggca
    34861 gaacaattaa aaaaagaaca tcctgatttg acgaaagata cggtcaccga tgtcgtacaa
    34921 aaatctgtag gtcaggtttt tgcacgtgta ctggaagacg ctggagtatt caagcgagat
    34981 gaaaaaggac aggcagcgtt tgaacgattt actgcaattt tataaacgta aaaaaggagg
    35041 agagacaaat ggcaacaatt aaagacattg cgcagttggc aggagtttct cctgctactg
    35101 tttcaagagt tctcaactat gatcctgatc tttccgttgg cttagccaca aagcaaaaaa
    35161 tttttgaagc agcagaacgc ttaaattata cgaaacatcg taaaaatgca gcaaatacta
    35221 agcaaacgct tcgcctgatc cagtggtacg acgatacgga agagcttgaa gatctttatt
    35281 atttatctat tcgtctagga atcgaaaaaa aggcagaaga agcaaacgtt actttattga
    35341 aagaaacgtg ggattccatt agtgatcttc cgacagatgg aacgatcgca ctaggaaaat
    35401 tcgacgaagc tcagttatta gaaatgaaag gatcccaaaa tgccttgctt ttggtagatt
    35461 ctgacggcac ttctcaagga atcgactctc tagttgtcga ctttaagtca agtgttcaaa
    35521 aagtcattga acattttgtc tcccataaag cgcaacatat cgctatgctt gctggaacag
    35581 aatatacgaa gaaaaaccat cagcggctaa aagatcccag aattatggca tttcaacaag
    35641 ttttggcaga aaaaggctac gacgaaggtc caattatcga agcagatttt tctgtcgagt
    35701 caggatatca agcagtgaag cagtatttaa aaacgaatcc gcatgttcct gatgcgcttt
    35761 ttgcagctaa tgatgcatta gcgattggcg cactgaaagc gattcaagaa aaaggtttag
    35821 tggtaccaga agatatttct gtgatgggtt tcaatgatgt cagtgtagcg aaatatgtaa
    35881 cgccctctct ttctactgta aaagtttata ccgaatggat gggagagcta gcagtggaga
    35941 cgattcttca attaagtaat tccaaagcac cggtttcaag aaagattatt gtggaaacag
    36001 agttgattat tcgagaatca acgaaataaa agatggaaaa gctgtgggga aatgaagtga
    36061 taccgcagct tttttgtgtt tcaggaaggg gcaaaatttg cccgccagca gataatcatg
    36121 tatcattgta ctagttttgc ctttggtatt ttcatcctca gaataaaaaa gtatgctaca
    36181 atggaaggcg agaatgtcat agcagaaagg atcaaatata tgattacatt aggaatagat
    36241 actgcaaacc aaactttggc gatcggtgtg gtagaagacg aacaaattct tggacaaata
    36301 caaacaaata tcaaacgcaa ccacagtgta acactgatgc ctgcaatcga ccaattgttt
    36361 gcagatcttc aaataagtcc aaaagacatt gaccgaatcg cggtttctga tggtcccggt
    36421 tcttatacag ggcttcgtat aggtgtaacg acagcaaaaa cgattgctta tacattagat
    36481 aaagagctag taggagtatc tagtctgaaa accatcgcgg ctaattgcat cggtgtggat
    36541 aaaattattg tgcccatgtt tgatgctcga agaaaaaatg tatacgctgg ggcataccgc
    36601 tggaaagaaa atagattaga aattgtactt gaagatgtac atatttctgc ttctgatttg
    36661 ttcacacaac tacaaaagct gaatgaagct atttatttcg tcgggagtga ttgtcacaaa
    36721 ttcttctatg aaataaagga aatattgcct agtgcgatga ttaatacagt tccagcatgg
    36781 gatattccaa gtggtgtaac tgtggctcaa ttaggtgctt tagctgaacc agttgcagag
    36841 aaacaagctt ttcttcctcg ctatttaaaa cgggtagaag cagaagaaaa atggttggag
    36901 acgcacacac ctggagacga aagttatgtt gaaaaaattt agagcgtatg ccagaagctt
    36961 ttttcaagat aaaatgattt atacagaaaa aacaatcgaa caaatcgatc gtcgttacgt
    37021 actaagagaa ttgactacag aagatatcaa agagctgctg gatgtcgaac gagaagtcta
    37081 taatggcgaa ttgccatgga caaaaagtgc ctttatatca gaattgaagt caccgcttcc
    37141 tcatctatat ctaggtatct ttgataatgg aagaatgatt ggttttatcg gttcaagagc
    37201 gttcggttta gattgccata tcacgaatat tgccgttcga ccggcatatc agcgtaaacg
    37261 aatcggttcg ctgcttattg acgaaatcga gaactttgct ataatgaatc gctgtgagac
    37321 gatgtcatta gaagtgcgta tgagtaatca agatgcacaa agactttatc ggaagttagg
    37381 ttttcaagca acaacgatca aaaaaagata ttacactgaa acaaaagaag atgcgttgga
    37441 tatgataaaa cttttagaaa atgagtgaga ttagttgatt tgcagaaaaa aagattttga
    37501 tgaagcgtac ttggcagaat gcgtatggat gattagcgaa aatgcttatc cacatggttc
    37561 accttggacg aaaatgcagt ttttggcaga catccaacag cctcactctg attatctgct
    37621 tttagttgaa gataaccaga tctgtggctt tattggatac tcgaaagtaa tagatgaagt
    37681 cgagatcaca aatattgctg ttgcagtttc agaacaagga aaagggcatg cacggcattt
    37741 gttacaatta ctgattacgg aacaaataaa agattcgctg catgtttttt tagaagtacg
    37801 tctatctaat gaagcagcaa gaaaacttta tgaatcagaa aagtttcgta tattaggtaa
    37861 acggaaatca tattatcgca atccgacgga agatgcgatc attatgagca gaaagttaaa
    37921 aacggagacg atgaaatgaa tgaagaattg attctggcga ttgaatcaag ttgtgatgaa
    37981 acgagtgtag cggtcgttag aaatggaaca gagatcttat ctaacatcgt tgcttctcaa
    38041 ataaacagtc ataaacgttt tggtggcgta gttcctgaag tagctagccg ccatcacgta
    38101 gaacaaatca ccttatgctt ggaagatgca ctggtagaag ctggtgtgtc ggcagaagat
    38161 ttatcggcag tcgctgtcac ttatggacca ggacttgtcg gctcgttgct gattggtatt
    38221 tctgcagcta aggcttttgc ttgggcacac caattgccgc ttattcctgt aaaccatatg
    38281 gcagggcata tctatgcagc ccgtctggtt aaaccattcc agtttccatt gatggcactt
    38341 cttgttagcg gaggacatac agaattagtc tatatgcaag aagatggatc atatgaaatc
    38401 attggggaga caagagatga cgcagcagga gaagcttatg acaaagttgg aagagtatta
    38461 ggtctttctt acccaagcgg aaaagaaatc gatcagctag ctcatcaggg aaaagataat
    38521 taccattttc cacgagcgat gatccatgaa gataattatg atttcagttt tagcggatta
    38581 aaaagtgcat ttatcaattt ggtgcataat gcacaacagc gtggagaaga tctagacaaa
    38641 aatgatttag cagctagctt tcaagcaagt gtgatcgatg tattgattac taaaacactt
    38701 cgagcttgtc aaaattatcc agtcaagcaa ctgattgtag ctggaggtgt agcggccaat
    38761 caagggttgc gtgaaggatt gcaggatgct ttggcaacta aactccctga ggtagaacta
    38821 gtgattcccc ctcttcgact ttgtggagat aatgctgcaa tgattggagc agcagcacat
    38881 gtggaaatgc aaaaaagaaa ttttgctagc tatcagttga atgctgatcc aagcttggtt
    38941 ttatctgaat aaagaggttg tgaaaaatct gccctgcatc aagtaataag aacagcccag
    39001 gaaaaacggc ttctcatgtt tttgacctgg ctgttcttat taccgcgtat gcaagctttt
    39061 gaacgccgtt taatcaagag ttcgtaagcc tgtcgtttaa ctctgattca caaggaacaa
    39121 tttcaaaaaa cagctcctca tgtttatgaa cattatgact tgtgaccgaa gagttgacag
    39181 gtacacaccg tttaatcgaa ataagggctg tgacacgctt ttgtcaaagc cctatttttt
    39241 acagcttatt catcattatc cgttcctctg attgccctca ctgattaatc gtttattgtc
    39301 gaggaatatc gaataatcta ggtcggatga ggacaagacg cttcctctag ttaatctatc
    39361 agtatttttt gcaaacagtg aatattacat aactgaaaca cacatagaaa atctttgagt
    39421 cttgcccagt gatctaaaac tgccggatct ttcagaagtt ttagggagga aaataagcag
    39481 tgaccctgat aaatatcagg gtcactgctt atttttgttc gcgttattct ctcatagctg
    39541 gagtagtagc tgtcttacag aaagtaaaac gctttttgtc ataatctaac agtccggaat
    39601 cacgtagttg tttgattact tgtccagcag tttcacgtgt cgtgccgcta ttcactgcta
    39661 attcttttaa ggtgatcgga taaggaatgg tgatatgacc atcttttgtt ggtacgccca
    39721 attcttcttt caaaatgaat aaattttgtt ttacccggtc aaaggcgcta gaggtcaatc
    39781 cgatttggat acgcaattca tgtatttcaa ctactttaga aattttttgt atcatataga
    39841 tcaactgttc gggatttttt cgtgctaatt tttcatagta ggaaacagga aggtagaatg
    39901 tttcaatatc agtcatcgct tgagctgtat aagtgtaggt ctcttctcca aacattcctg
    39961 acataggaaa aaggttattt gtattcacat agtcatagta gtaataagtt gcactttcat
    40021 cataacgttc taaacgaacg agtcctttgg tcaagatata caaccgttgt ctgggatcac
    40081 catcgtcgaa aaggatctgt ccttttttat aagaacgaag aacagtatgt tccttgatct
    40141 gttggaactc ttcatccgag aaatgttcaa aatcaggttg atatctgagt tttgaaaagt
    40201 gaaaatcgat tttattaaca cgcattaaaa agtcctctcc ttattgttca ctatatttat
    40261 tgtcactttt tttaaagaat ttgtaaag
//
LOCUS       DACCWK010000017        39855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCWK010000017 DACCWK010000000
VERSION     DACCWK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.017"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..369
                     /locus_tag="IVW65_000808"
                     /pseudo
     CDS             1..369
                     /locus_tag="IVW65_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(495..1574)
                     /locus_tag="IVW65_000809"
     CDS             complement(495..1574)
                     /locus_tag="IVW65_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7899729.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1677..2117)
                     /locus_tag="IVW65_000810"
     CDS             complement(1677..2117)
                     /locus_tag="IVW65_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7899730.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2174..2584)
                     /locus_tag="IVW65_000811"
     CDS             complement(2174..2584)
                     /locus_tag="IVW65_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7899731.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2764..3198)
                     /locus_tag="IVW65_000812"
     CDS             complement(2764..3198)
                     /locus_tag="IVW65_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7899732.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3298..4146)
                     /locus_tag="IVW65_000813"
     CDS             complement(3298..4146)
                     /locus_tag="IVW65_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7899733.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4535..6301
                     /locus_tag="IVW65_000814"
     CDS             4535..6301
                     /locus_tag="IVW65_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7899734.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6403..7662
                     /locus_tag="IVW65_000815"
     CDS             6403..7662
                     /locus_tag="IVW65_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7899735.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7809..9110
                     /locus_tag="IVW65_000816"
     CDS             7809..9110
                     /locus_tag="IVW65_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7899736.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9113..9964
                     /locus_tag="IVW65_000817"
     CDS             9113..9964
                     /locus_tag="IVW65_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7899737.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9977..10792
                     /locus_tag="IVW65_000818"
     CDS             9977..10792
                     /locus_tag="IVW65_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7899738.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10943..12109
                     /locus_tag="IVW65_000819"
     CDS             10943..12109
                     /locus_tag="IVW65_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7899739.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12339..12986
                     /locus_tag="IVW65_000820"
     CDS             12339..12986
                     /locus_tag="IVW65_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7899740.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13070..14014
                     /locus_tag="IVW65_000821"
     CDS             13070..14014
                     /locus_tag="IVW65_000821"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7899741.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14149..15600
                     /locus_tag="IVW65_000822"
     CDS             14149..15600
                     /locus_tag="IVW65_000822"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7899742.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15621..16457
                     /locus_tag="IVW65_000823"
     CDS             15621..16457
                     /locus_tag="IVW65_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7899743.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16457..17116
                     /locus_tag="IVW65_000824"
     CDS             16457..17116
                     /locus_tag="IVW65_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7899744.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            17242..17931
                     /locus_tag="IVW65_000825"
     CDS             17242..17931
                     /locus_tag="IVW65_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899745.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17937..18893
                     /locus_tag="IVW65_000826"
     CDS             17937..18893
                     /locus_tag="IVW65_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7899746.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18937..19449
                     /locus_tag="IVW65_000827"
     CDS             18937..19449
                     /locus_tag="IVW65_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7899747.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19705..20253)
                     /locus_tag="IVW65_000828"
     CDS             complement(19705..20253)
                     /locus_tag="IVW65_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7899748.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(20342..21523)
                     /locus_tag="IVW65_000829"
     CDS             complement(20342..21523)
                     /locus_tag="IVW65_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7899749.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21759..23267)
                     /locus_tag="IVW65_000830"
     CDS             complement(21759..23267)
                     /locus_tag="IVW65_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7899750.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            23426..23617
                     /locus_tag="IVW65_000831"
     CDS             23426..23617
                     /locus_tag="IVW65_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899751.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23820..24737
                     /locus_tag="IVW65_000832"
     CDS             23820..24737
                     /locus_tag="IVW65_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7899752.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24730..25803
                     /locus_tag="IVW65_000833"
     CDS             24730..25803
                     /locus_tag="IVW65_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899753.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            25951..26469
                     /locus_tag="IVW65_000834"
     CDS             25951..26469
                     /locus_tag="IVW65_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899754.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            26450..26842
                     /locus_tag="IVW65_000835"
     CDS             26450..26842
                     /locus_tag="IVW65_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7899755.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26918..27853
                     /locus_tag="IVW65_000836"
     CDS             26918..27853
                     /locus_tag="IVW65_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7899756.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            27986..28765
                     /locus_tag="IVW65_000837"
     CDS             27986..28765
                     /locus_tag="IVW65_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7899757.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28758..29075
                     /locus_tag="IVW65_000838"
     CDS             28758..29075
                     /locus_tag="IVW65_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7899758.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            29319..30929
                     /locus_tag="IVW65_000839"
     CDS             29319..30929
                     /locus_tag="IVW65_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7899759.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31086..31934
                     /locus_tag="IVW65_000840"
     CDS             31086..31934
                     /locus_tag="IVW65_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7899760.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            32165..32644
                     /locus_tag="IVW65_000841"
     CDS             32165..32644
                     /locus_tag="IVW65_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899761.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            32677..34905
                     /locus_tag="IVW65_000842"
     CDS             32677..34905
                     /locus_tag="IVW65_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7899762.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            35034..36677
                     /locus_tag="IVW65_000843"
     CDS             35034..36677
                     /locus_tag="IVW65_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7899763.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(36750..37295)
                     /locus_tag="IVW65_000844"
     CDS             complement(36750..37295)
                     /locus_tag="IVW65_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7899764.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(37318..37803)
                     /locus_tag="IVW65_000845"
     CDS             complement(37318..37803)
                     /locus_tag="IVW65_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7899765.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(37793..38605)
                     /locus_tag="IVW65_000846"
     CDS             complement(37793..38605)
                     /locus_tag="IVW65_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7899766.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(38744..>39855)
                     /locus_tag="IVW65_000847"
     CDS             complement(38744..>39855)
                     /locus_tag="IVW65_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7899767.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13177 a         7200 c         7810 g        11668 t
ORIGIN      
        1 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
       61 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
      121 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
      181 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
      241 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
      301 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
      361 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
      421 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
      481 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
      541 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
      601 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
      661 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
      721 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
      781 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
      841 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
      901 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
      961 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     1021 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     1081 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     1141 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     1201 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     1261 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     1321 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     1381 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     1441 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     1501 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     1561 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     1621 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     1681 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     1741 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     1801 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     1861 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     1921 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     1981 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     2041 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     2101 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     2161 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     2221 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     2281 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     2341 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     2401 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     2461 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     2521 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     2581 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     2641 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     2701 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     2761 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     2821 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     2881 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     2941 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     3001 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     3061 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     3121 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     3181 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     3241 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     3301 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     3361 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     3421 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     3481 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     3541 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     3601 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     3661 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     3721 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     3781 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     3841 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     3901 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     3961 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     4021 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     4081 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     4141 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     4201 tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca
     4261 ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg
     4321 gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa
     4381 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
     4441 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt
     4501 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
     4561 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
     4621 taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac
     4681 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
     4741 ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt
     4801 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
     4861 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
     4921 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
     4981 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
     5041 ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac
     5101 ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
     5161 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
     5221 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
     5281 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
     5341 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     5401 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     5461 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     5521 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     5581 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     5641 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     5701 tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     5761 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     5821 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     5881 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     5941 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     6001 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     6061 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg
     6121 tgaagataag ctctatttca cacggaaact agagggacag atcatctatg tttactttaa
     6181 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     6241 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     6301 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     6361 taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg
     6421 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     6481 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     6541 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     6601 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     6661 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     6721 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     6781 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     6841 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     6901 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     6961 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     7021 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     7081 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     7141 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     7201 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     7261 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     7321 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     7381 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     7441 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     7501 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     7561 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     7621 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     7681 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     7741 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     7801 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     7861 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     7921 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     7981 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     8041 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     8101 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     8161 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     8221 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     8281 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     8341 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     8401 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     8461 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     8521 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     8581 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     8641 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     8701 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     8761 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     8821 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     8881 atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg
     8941 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     9001 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     9061 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     9121 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     9181 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     9241 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     9301 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     9361 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     9421 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     9481 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     9541 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     9601 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     9661 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     9721 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     9781 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     9841 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     9901 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     9961 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
    10021 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
    10081 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
    10141 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
    10201 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
    10261 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
    10321 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
    10381 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
    10441 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
    10501 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
    10561 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
    10621 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
    10681 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
    10741 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
    10801 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
    10861 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
    10921 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
    10981 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
    11041 tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
    11101 tacaagtaac gcatctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
    11161 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
    11221 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
    11281 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
    11341 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
    11401 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
    11461 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
    11521 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
    11581 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
    11641 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
    11701 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
    11761 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
    11821 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
    11881 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
    11941 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
    12001 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
    12061 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
    12121 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
    12181 ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata
    12241 ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg
    12301 taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata
    12361 ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat
    12421 ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa
    12481 aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag
    12541 atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg
    12601 caattactag tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg
    12661 tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag
    12721 agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc
    12781 tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag
    12841 gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag
    12901 tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag
    12961 tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt
    13021 ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga
    13081 aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg
    13141 catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa
    13201 gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt
    13261 agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc
    13321 tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg
    13381 gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg
    13441 gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga
    13501 ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg
    13561 aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa
    13621 tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga
    13681 tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga
    13741 gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga
    13801 aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct
    13861 atgtggcgca agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt
    13921 ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga
    13981 cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc
    14041 ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta
    14101 tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca
    14161 acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat
    14221 atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca
    14281 ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt
    14341 atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga
    14401 tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa
    14461 gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt
    14521 acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc
    14581 ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct
    14641 agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa
    14701 ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct
    14761 attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga
    14821 agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga
    14881 aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa
    14941 gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg
    15001 acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat
    15061 ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga
    15121 aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc
    15181 gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta
    15241 catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc
    15301 gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa
    15361 gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat
    15421 acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa
    15481 gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt
    15541 caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag
    15601 cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct
    15661 tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta
    15721 atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt
    15781 attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta
    15841 aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg
    15901 tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa
    15961 aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa
    16021 aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac
    16081 ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca
    16141 atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa
    16201 atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac
    16261 tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg
    16321 ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga
    16381 aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg
    16441 agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact
    16501 tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt
    16561 aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa
    16621 tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt
    16681 tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt
    16741 tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc
    16801 ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata
    16861 tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga
    16921 tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc
    16981 aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat
    17041 ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat
    17101 gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt
    17161 tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa
    17221 atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta
    17281 taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac
    17341 caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga
    17401 tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt
    17461 ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc
    17521 cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc
    17581 tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa
    17641 gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg
    17701 gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca
    17761 gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct
    17821 agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat
    17881 ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt
    17941 actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc
    18001 gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc
    18061 ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg aatcgctgtg atccaaaatc
    18121 cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa
    18181 atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt
    18241 cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga
    18301 aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct
    18361 ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa
    18421 gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg
    18481 gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc
    18541 ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga
    18601 aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag
    18661 caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag
    18721 gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga
    18781 aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat catccaaaag
    18841 attggcttaa atattattca aaagttcagg aactatatga agtgatccaa tgattagata
    18901 atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa
    18961 gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa
    19021 ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt
    19081 gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta
    19141 gtctcaagcg tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt
    19201 ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca
    19261 aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt
    19321 atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact
    19381 tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc
    19441 atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac
    19501 aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa
    19561 tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct
    19621 ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta
    19681 tcctacgagt attattcgat acatttatat caataatcct ttttttattt tatctgcagc
    19741 ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt
    19801 caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc
    19861 tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg
    19921 gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta
    19981 acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg
    20041 agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc
    20101 agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta
    20161 agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc
    20221 agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca
    20281 agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat
    20341 attataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca
    20401 aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg
    20461 caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa
    20521 ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa
    20581 attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg
    20641 ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga
    20701 tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa
    20761 gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt
    20821 ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata
    20881 catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag
    20941 tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg
    21001 tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct
    21061 tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag
    21121 attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata
    21181 atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca
    21241 tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt
    21301 caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa
    21361 ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca
    21421 tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc
    21481 ctaatgtgac aaataaacca ataataccta ataaataact caaataatca ctcctattcc
    21541 aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca
    21601 ttactttttt agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa
    21661 aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc
    21721 taatagtttc gttttcaaca tttataagta aactttttct attggtaata tagattttcg
    21781 gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca
    21841 tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca
    21901 taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca
    21961 ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt
    22021 tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact
    22081 gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg
    22141 acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct
    22201 tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa
    22261 ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt
    22321 ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg
    22381 acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat
    22441 tgataaacag attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc
    22501 tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg
    22561 atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt
    22621 ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct
    22681 ttttggcttt catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca
    22741 ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca
    22801 tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag
    22861 accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta
    22921 tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc atcgattaac
    22981 cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata
    23041 tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg
    23101 attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc
    23161 ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct
    23221 gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct
    23281 taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc
    23341 tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga
    23401 tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa
    23461 acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga
    23521 acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg
    23581 cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga
    23641 ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt
    23701 ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa
    23761 aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta
    23821 tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg
    23881 atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga
    23941 atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata
    24001 tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa
    24061 atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg
    24121 aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg
    24181 gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg
    24241 ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg
    24301 acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt
    24361 tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat
    24421 tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac
    24481 tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc
    24541 aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa
    24601 aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc
    24661 atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag
    24721 gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa
    24781 gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca
    24841 gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg
    24901 aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta
    24961 gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa
    25021 ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa
    25081 tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg
    25141 atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca
    25201 aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga
    25261 caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa
    25321 tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg
    25381 gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg
    25441 atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt
    25501 aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa
    25561 ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat
    25621 atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg
    25681 ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga
    25741 tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc
    25801 tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg
    25861 gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa
    25921 cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc
    25981 ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag
    26041 ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt
    26101 gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc
    26161 ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa
    26221 acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac
    26281 ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat
    26341 taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt
    26401 gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat
    26461 aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac
    26521 ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat
    26581 cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga
    26641 tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac
    26701 ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga
    26761 cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat
    26821 tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata
    26881 attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg
    26941 aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt
    27001 cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga
    27061 ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga
    27121 gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat
    27181 gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc
    27241 atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat
    27301 cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact
    27361 ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga
    27421 agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc
    27481 aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg
    27541 agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc
    27601 aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga
    27661 tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt
    27721 tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca
    27781 gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta
    27841 ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg
    27901 tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt
    27961 tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc
    28021 tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt
    28081 attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt
    28141 ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac
    28201 gacattattg atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc
    28261 cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga
    28321 tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa
    28381 ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa
    28441 ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat
    28501 gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt
    28561 ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc
    28621 agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa
    28681 agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga
    28741 acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca
    28801 tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat
    28861 cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta
    28921 aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa
    28981 tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag
    29041 gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga
    29101 gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct
    29161 ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt
    29221 ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa
    29281 gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag
    29341 gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga
    29401 ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata
    29461 atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg
    29521 cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga
    29581 tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt
    29641 atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac
    29701 tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag
    29761 gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg
    29821 ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa
    29881 ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga
    29941 ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga
    30001 tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa
    30061 cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa
    30121 cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct
    30181 tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag
    30241 gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac
    30301 aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat
    30361 tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg
    30421 aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga
    30481 tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag
    30541 aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg
    30601 ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta
    30661 tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt
    30721 tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc
    30781 gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg
    30841 gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg
    30901 ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa
    30961 aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat
    31021 ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta
    31081 aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga
    31141 accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg
    31201 aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc
    31261 ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga
    31321 aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca
    31381 cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga
    31441 tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc
    31501 agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc
    31561 tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc
    31621 tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt
    31681 tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta
    31741 gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag
    31801 aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa
    31861 ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca
    31921 tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt
    31981 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
    32041 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
    32101 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
    32161 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
    32221 taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac tgggccttgc
    32281 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
    32341 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
    32401 agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
    32461 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
    32521 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
    32581 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
    32641 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
    32701 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
    32761 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
    32821 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
    32881 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
    32941 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
    33001 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
    33061 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
    33121 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
    33181 cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat ccgagatgcc
    33241 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
    33301 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
    33361 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
    33421 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
    33481 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
    33541 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
    33601 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
    33661 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
    33721 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa aggaagaacc
    33781 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
    33841 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
    33901 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
    33961 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
    34021 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
    34081 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
    34141 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
    34201 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
    34261 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
    34321 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
    34381 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
    34441 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
    34501 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
    34561 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
    34621 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
    34681 ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
    34741 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
    34801 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
    34861 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
    34921 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
    34981 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
    35041 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
    35101 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
    35161 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
    35221 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
    35281 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
    35341 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
    35401 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
    35461 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
    35521 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
    35581 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
    35641 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
    35701 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
    35761 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
    35821 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
    35881 aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc gcttggcaaa
    35941 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
    36001 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
    36061 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
    36121 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
    36181 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
    36241 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
    36301 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
    36361 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
    36421 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
    36481 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
    36541 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
    36601 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
    36661 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
    36721 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
    36781 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
    36841 acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat atcgataccg
    36901 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
    36961 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
    37021 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
    37081 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
    37141 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
    37201 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
    37261 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
    37321 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
    37381 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
    37441 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
    37501 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
    37561 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
    37621 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
    37681 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
    37741 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
    37801 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
    37861 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
    37921 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
    37981 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
    38041 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
    38101 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
    38161 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
    38221 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
    38281 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
    38341 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
    38401 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
    38461 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
    38521 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
    38581 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
    38641 tttcaaaaaa gaaaaacagc caattggcat attttcatct catccaattt aaaaaaacaa
    38701 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
    38761 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
    38821 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
    38881 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
    38941 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
    39001 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
    39061 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
    39121 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
    39181 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
    39241 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
    39301 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
    39361 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
    39421 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
    39481 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
    39541 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
    39601 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
    39661 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
    39721 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
    39781 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
    39841 aataaagaat aggaa
//
LOCUS       DACCWK010000018        38526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCWK010000018 DACCWK010000000
VERSION     DACCWK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.018"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(112..630)
                     /locus_tag="IVW65_000848"
     CDS             complement(112..630)
                     /locus_tag="IVW65_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7899768.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(632..1249)
                     /locus_tag="IVW65_000849"
     CDS             complement(632..1249)
                     /locus_tag="IVW65_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7899769.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1246..2109)
                     /locus_tag="IVW65_000850"
     CDS             complement(1246..2109)
                     /locus_tag="IVW65_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7899770.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(2096..3007)
                     /locus_tag="IVW65_000851"
     CDS             complement(2096..3007)
                     /locus_tag="IVW65_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7899771.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3048..4382)
                     /locus_tag="IVW65_000852"
     CDS             complement(3048..4382)
                     /locus_tag="IVW65_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7899772.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4590..5420
                     /locus_tag="IVW65_000853"
     CDS             4590..5420
                     /locus_tag="IVW65_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7899773.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5664..5913)
                     /locus_tag="IVW65_000854"
                     /pseudo
     CDS             complement(5664..5913)
                     /locus_tag="IVW65_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6104..6298)
                     /locus_tag="IVW65_000855"
     CDS             complement(6104..6298)
                     /locus_tag="IVW65_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899774.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6803..6988)
                     /locus_tag="IVW65_000856"
     CDS             complement(6803..6988)
                     /locus_tag="IVW65_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899775.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7838..8179)
                     /locus_tag="IVW65_000857"
     CDS             complement(7838..8179)
                     /locus_tag="IVW65_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7899776.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8179..8409)
                     /locus_tag="IVW65_000858"
     CDS             complement(8179..8409)
                     /locus_tag="IVW65_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7899777.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8696..9034
                     /locus_tag="IVW65_000859"
     CDS             8696..9034
                     /locus_tag="IVW65_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899778.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9239..9478)
                     /locus_tag="IVW65_000860"
     CDS             complement(9239..9478)
                     /locus_tag="IVW65_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7899779.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9454..9801)
                     /locus_tag="IVW65_000861"
     CDS             complement(9454..9801)
                     /locus_tag="IVW65_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7899780.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10533..11114)
                     /locus_tag="IVW65_000862"
     CDS             complement(10533..11114)
                     /locus_tag="IVW65_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7899781.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11297..11923)
                     /locus_tag="IVW65_000863"
     CDS             complement(11297..11923)
                     /locus_tag="IVW65_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7899782.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11945..13276)
                     /locus_tag="IVW65_000864"
     CDS             complement(11945..13276)
                     /locus_tag="IVW65_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7899783.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13431..13832)
                     /locus_tag="IVW65_000865"
     CDS             complement(13431..13832)
                     /locus_tag="IVW65_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7899784.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13918..14124)
                     /locus_tag="IVW65_000866"
     CDS             complement(13918..14124)
                     /locus_tag="IVW65_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899785.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14219..15721)
                     /locus_tag="IVW65_000867"
     CDS             complement(14219..15721)
                     /locus_tag="IVW65_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7899786.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15739..16194)
                     /gene="argR"
                     /locus_tag="IVW65_000868"
     CDS             complement(15739..16194)
                     /gene="argR"
                     /locus_tag="IVW65_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7899787.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16292..16909)
                     /locus_tag="IVW65_000869"
     CDS             complement(16292..16909)
                     /locus_tag="IVW65_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899788.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17130..18503
                     /locus_tag="IVW65_000870"
     CDS             17130..18503
                     /locus_tag="IVW65_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7899789.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18578..19690
                     /locus_tag="IVW65_000871"
     CDS             18578..19690
                     /locus_tag="IVW65_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7899790.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19762..21399)
                     /locus_tag="IVW65_000872"
     CDS             complement(19762..21399)
                     /locus_tag="IVW65_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7899791.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21567..22175)
                     /locus_tag="IVW65_000873"
     CDS             complement(21567..22175)
                     /locus_tag="IVW65_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7899792.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(22559..23710)
                     /gene="queG"
                     /locus_tag="IVW65_000874"
     CDS             complement(22559..23710)
                     /gene="queG"
                     /locus_tag="IVW65_000874"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7899793.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(23729..24481)
                     /locus_tag="IVW65_000875"
     CDS             complement(23729..24481)
                     /locus_tag="IVW65_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899794.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(24622..25572)
                     /locus_tag="IVW65_000876"
     CDS             complement(24622..25572)
                     /locus_tag="IVW65_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7899795.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(25573..26412)
                     /locus_tag="IVW65_000877"
     CDS             complement(25573..26412)
                     /locus_tag="IVW65_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7899796.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(26409..27155)
                     /locus_tag="IVW65_000878"
     CDS             complement(26409..27155)
                     /locus_tag="IVW65_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7899797.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            27393..28763
                     /locus_tag="IVW65_000879"
     CDS             27393..28763
                     /locus_tag="IVW65_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7899798.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(28818..29714)
                     /locus_tag="IVW65_000880"
     CDS             complement(28818..29714)
                     /locus_tag="IVW65_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7899799.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(29995..30672)
                     /locus_tag="IVW65_000881"
     CDS             complement(29995..30672)
                     /locus_tag="IVW65_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7899800.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(30763..31668)
                     /locus_tag="IVW65_000882"
     CDS             complement(30763..31668)
                     /locus_tag="IVW65_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7899801.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            31843..33297
                     /locus_tag="IVW65_000883"
     CDS             31843..33297
                     /locus_tag="IVW65_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7899802.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            33313..34344
                     /locus_tag="IVW65_000884"
     CDS             33313..34344
                     /locus_tag="IVW65_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7899803.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            34351..35538
                     /locus_tag="IVW65_000885"
     CDS             34351..35538
                     /locus_tag="IVW65_000885"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7899804.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            35694..37076
                     /locus_tag="IVW65_000886"
     CDS             35694..37076
                     /locus_tag="IVW65_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7899805.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            37203..37433
                     /locus_tag="IVW65_000887"
     CDS             37203..37433
                     /locus_tag="IVW65_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899806.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(37633..38301)
                     /locus_tag="IVW65_000888"
     CDS             complement(37633..38301)
                     /locus_tag="IVW65_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7899807.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        11201 a         7791 c         6899 g        12635 t
ORIGIN      
        1 acaaaactta agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa
       61 ataaagcaca aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac
      121 ggcagcgcat aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg
      181 ataatgtaaa agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa
      241 tattcctgca actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt
      301 atgtcctgaa ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt
      361 tttttcataa ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa
      421 atagatcaaa ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac
      481 taaaacagca gcaaggagca accaaataaa accaccatta gagatattac tgataaattt
      541 caaaaaattg atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact
      601 atcatcgatt ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt
      661 gcgtacgatg gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag
      721 caaaatagat tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat
      781 agaaaagcgc gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt
      841 ctttttgttc cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca
      901 aaaacaacgt agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct
      961 gataatcgat caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa
     1021 gagagaagtt ctcacgaaac tttttccaga atagtgaacc gagctttttc tcttcatttt
     1081 ttttgtaatg ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag
     1141 gcaagacgca caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga
     1201 tttctaagaa ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc
     1261 tcctgtcaaa tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt
     1321 cgggatcaag acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt
     1381 ctgaaaaact tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag
     1441 aggacctaag aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc
     1501 aggtttcgtc aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat
     1561 ttcagcagct tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat
     1621 attgaacgca ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc
     1681 gatcttggag aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt
     1741 agaaagaacc aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata
     1801 agcaataaaa gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt
     1861 atttcttgca tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac
     1921 gattttttct gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga
     1981 agtaaacacc atccaaagca atggaatgat caaaatgatt cctattactg tcatgaggac
     2041 ataacttagt atttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt
     2101 tgccgtcacc tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca
     2161 gaaccaacac gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag
     2221 ctacgaacgc cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc
     2281 ctgttccatt tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag
     2341 aggtgatcac atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact
     2401 ttccccaaac gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt
     2461 gtaatgtagc taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca
     2521 acgcaggttt cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc
     2581 ccagaaatgt gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga
     2641 ctgctactcc tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt
     2701 tgattttttg attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat
     2761 tgaagatgac gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga
     2821 acattctttt gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg
     2881 tgaaactacc aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa
     2941 taaaaggcga gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac
     3001 gcttcaaatt ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc
     3061 ttgactaagt tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt
     3121 cctaaaagaa tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa
     3181 ggaactggtg tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt
     3241 cctttatccg ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg
     3301 atatcaaaac ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg
     3361 aattcataag aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca
     3421 ccccacgacc aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca
     3481 aaatcatccg gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg
     3541 gctactaatc cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta
     3601 ttgatcgtat ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact
     3661 ttatttttag tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct
     3721 gcgtttagcg cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta
     3781 aattcttttc cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt
     3841 gtcggtagat cttgttcact gattccagct tctttaaata tctttttatt ataaaaaatg
     3901 acttgtgtat ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc
     3961 caaagctgcg gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt
     4021 gcttgctgtg cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt
     4081 gcatttccag ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac
     4141 ttataattta catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata
     4201 gcttcttttc gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca
     4261 cttgtttttt cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg
     4321 catcctccta ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc
     4381 atattttacc tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt
     4441 gttctgaaaa taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac
     4501 tttatataat tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat
     4561 gtattaaaaa tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg
     4621 aagcaaaacc agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg
     4681 gccaatatgg cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag
     4741 gagaagaaaa atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg
     4801 aaatgttggc gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag
     4861 cagcgatgca aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg
     4921 cgatcgtagc tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg
     4981 cctatatcat tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat
     5041 cacaaggaag acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag
     5101 atatgctgtc tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag
     5161 tgaaagaatt agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca
     5221 aagaacgaaa agacattgct tatgacttct ataacttctc agagggagac caaagtagtg
     5281 aaggttcttg ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag
     5341 aacctaagat cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga
     5401 cacctccatt caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc
     5461 tttgtgagca aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc
     5521 tctagagcta taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta
     5581 tgttcaagcc atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact
     5641 ccttagccct taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca
     5701 tgtttttctt gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc
     5761 acatagtatc cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa
     5821 tctcttgatg atctcctgaa tattggctcg atattgatta taaattattt ttcttctata
     5881 ctttggtgtg aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc
     5941 ctttattgct ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat
     6001 taggtctaaa gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt
     6061 cagaaaaaaa tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt
     6121 tataaatgga aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta
     6181 tgaaatcttt atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat
     6241 ttatcagaag tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag
     6301 actctccttt cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa
     6361 aggggcgcag aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt
     6421 ttaataacta taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc
     6481 tccaattttt aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact
     6541 tatatatcga acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac
     6601 ttgcatctaa ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt
     6661 aatttttaaa ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc
     6721 tgtctcatcc agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg
     6781 aatagttgag ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata
     6841 caatgaaaaa caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact
     6901 cattactgta tgattttagc agcccaacca acatataccc tataaaagct aataagatgg
     6961 ttaggaatcc tttagaattt ttactcattc atagacactt ccttaaataa cttttattat
     7021 agaaggtatt gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaccttaca
     7081 ttatactgga atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg
     7141 taatcggtaa agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat
     7201 cataaaaggt acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc
     7261 tgacccaatt gcacattttc gatcattaca agtattggtg tcgttagaac aactaaataa
     7321 aaactagcgg tagatttttc agaatctatg ctaatttctg tatggatgtt ccagtgatgg
     7381 caaatttctc ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt
     7441 aaactataga aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc
     7501 gatatagttt aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac
     7561 tgtcagttgt tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta
     7621 agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca
     7681 cctcaatcat tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatgcc
     7741 atcgaccaca ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt
     7801 tagcatataa aaatattata gagtttactt acaatgctta aaacatatac tgtataatct
     7861 gatcaatttt agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct
     7921 taaaatcaag agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag
     7981 gtaatgagta acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa
     8041 acattgtttt acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa
     8101 tttcttttcc tacggaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat
     8161 aaattctttc ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc
     8221 gtctttctca taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac
     8281 taacgtaatt gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt
     8341 ctttccgtta tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt
     8401 aacaagcatt atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa
     8461 attccccttt gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata
     8521 aatagaaata aattgtagcc agttcatttt ttctttaact aaaaaaataa tccaagaaac
     8581 attgaaccta tggatccaaa agctacccaa atactatagc taaccccccg ctaccttcaa
     8641 ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca acaggccatc ctatcatgcc
     8701 cgattataaa caaaaagctg gctattcctt ttgctctgac aaatttcaat tgggaaagtt
     8761 cacgaaacta gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat
     8821 agaaacattg gttcaacgaa tttcaactag acaagacttt gctattgtag aacgtgagat
     8881 cgtagctatt tttgtagaag aagggatttt acatgatcca actcatatca atgtggataa
     8941 atggcaacct cttatctata aattcagaga atatacaact gccaaccagt cgttaacttt
     9001 gaattttaga ttccaagaat tcaaagagtc ataatgataa acatcgttag gtataaataa
     9061 aaaagactcg cagcacgagt aaaaaacaaa tagaagctat acattagaaa ggtcgactgt
     9121 gaccctatag atgcagtata tgcagcttat gagcatttct tctgttctct ttgtttttgg
     9181 gatctgtgct gaagtctttt tcgttttttg ccgctctttc tttgaaaaat cgattatgtt
     9241 ataccttctc cattactaat tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa
     9301 tatctgttac ccaaggttcg atttgttctt cagtcaagac aagcggcatt cggtcatgga
     9361 ttggtgcgac tgtgcggtta ggttcagtag tcattatgat tgattctgcc attttttcat
     9421 cctgatggct acgataaaaa ccgccaatat acactatttt ttccttattt gaaaacataa
     9481 atttgtcttt ttcttcattc cactcattgg gaaaacacaa cgattttgca aaaatggttg
     9541 atgaaaggtc tttttttctt ctactgtctc tgcacgggca ttaatcatca attgtccttt
     9601 tttaaatccg gtaaatcccc acttcatgat ttccggagtg aggatgtttt cttcctcttt
     9661 tcttaacgtc acaacaagat ttgacggaaa aacttctcca gctgctaatt ctgctgcctg
     9721 cttcggttgt ttcgtctggg cttctcgata ataattctga aggagctctg actctagatc
     9781 aaaaaaataa cgaccacaca tcctaatccc tcccaatctt tttttctatc atacactgca
     9841 acaagaagtt cgcaccaaca aaacgctttc aatttttgta gaactaaaaa agttgtgaca
     9901 agtgacaaac tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat
     9961 attcaacaaa catgagaagc tgctttttga aattgttcct tgtgactcgg agtgaaacgg
    10021 caggcttacg acctctgggt aaacggtgtt caaaagccag tttctcggta ttaagccaaa
    10081 tttatgcaaa aatgtagaaa gctattttca caaaattttt cttaatactc gaaactaagc
    10141 gactttttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc
    10201 ccaaccaaac gaaaaatatg agcagctatt tttatttggc tgttcttatt acttgatgca
    10261 gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctaacc ttcggtatta
    10321 gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta atactcaggt
    10381 caaagcgctg atttcacaac ctctaataca cggtgttcca gaagtagctt cttcgaaaaa
    10441 taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    10501 aagctgacca cttctgtcac aacctctaat tattaaagaa ccttttgcga agttaacaat
    10561 ctttctttga tcaattgaaa attctgccta agtagtgata gatcctgtgt gactttcaac
    10621 aataagatac ttccctggat ttcagcaatc aacgctttgg cttgacggtc tgcttcttca
    10681 tcggacagtt tttcttcaga aagtgccagg atattttgtt tcgtatactt gacccattga
    10741 ttgactaatt ctagtagcaa cagacggtat tcttcatcca ctgtactcaa ctcaataatc
    10801 aaattaccta caggacagcc atgataagtg atctgtgtct catgatcagc gaaaatccag
    10861 tccaacattt cttctaatgc ttcttctggc tgttttccag catttaagat gtcttcgaac
    10921 aattctgtct gccatacgtc catatgcgca tggatcaccg ctagacaaac ttcttttttt
    10981 gaagaaaagt aataataaaa ttgacctttt cctgtatcag aagctgctag aatatctcgg
    11041 attgacgttt catgatagcc ttttttataa ataagatctt tggtcgcttc caagatcttt
    11101 tcctttgttt tcaatgatat tctccttttt catttctcat agtcctatat tatacagtaa
    11161 attcatcatt ttttcttctt aatcgtttaa aaaagactgt gccaaattat ctgacacagc
    11221 cctcactata aaactgttct catatagcta gatgttcatt gattcaagaa ctgcgaggac
    11281 aaaagcaatt cctatcttat gatagttgct ctaatacttc ctctgttgtc atcacttgat
    11341 ctgcaataaa gccaaagttc gtcaacgctg catccattgc ttcttggcca ggagcaccaa
    11401 ttgcatctgt cgctaccaca acattgaatc cactctctac caattcacgc atatgggaat
    11461 cgctacacaa gtttccgttc attccaccaa gaattactgt gtcgattcca ttttttctca
    11521 attggagcgc caaatcattg ctttctggtc caaagatttt atgaggagag ctgatgatcg
    11581 tattttcatc caaataaggt ttcaattctt cgataaaatc ggctcctgaa ccttcttcca
    11641 ctgattcata ttgacttttt ctagcaaaca tctgctgctc aagcatcata tgttctcctg
    11701 caccgccaaa ttgccattta tggtcatgtg ggtaaaaata atgaggagaa ataaagagtt
    11761 tgtatccttt ttcttttgct tgttttacca tttcaatcat attttcgatc gtattgtatt
    11821 tttctaagat atcttttgtc aaaccaaagc caccgcctgc tggtttcaag aactcgactt
    11881 gcgggtcagt gagaactact gctgtatttt tattattgaa catggtggat tctccattcg
    11941 ttatttattt taatacatca tttagagctt cactcattgt cggatgagaa tagatttgat
    12001 ctctcaataa cgtataagga agttttccat tcatagctag cgaaatcaag ttgatgactt
    12061 catgagaatc ttccgcatag atacttgccc ctagaatcaa gtcagtttca gggtcgacta
    12121 aaactttgaa tacaccttta ggatcttcta atacttgtgc tttagggaca ccgcttgtca
    12181 tatacttgaa taatttgtaa ttgacgcctt gtgctttggc ttgtttttct gttaaaccta
    12241 cattggataa agtcggagtg ataaatacac tgtaaggcac agctttccgg ttattcgttg
    12301 ttcgtttatt ctcgcccttc aactgatcca taatgattcg ataatcatct agagagatat
    12361 aagtgaactg caatccgcct ttgacgtctc caatcgccca aatgttgtct gcatctgtac
    12421 gaaggtattc atccacagcg attgctccgc gatctgttag tttaacatct gtattttcta
    12481 atccaagtga tgctgtactt ggttttcttc cagttgcagc caaaactttt gacgcttgga
    12541 ttgttacttc ttggccatct tctgtaaagg tgacggcagc tgttgattct tggtctgcta
    12601 cttgatctac agatacacct agatggaatt cgattcccgc atttgtcatg tcttctaaaa
    12661 tcatttgcgc gatatcttca tcttcacgtg gaagaaattc agaatgggca tcaagaacga
    12721 caactttaga tccgtattca ttgaacattg atgcaaattc caagccaata tatccagccc
    12781 cgatgatcac taatttttcc ggtaattgtt cctgatccat cgcttgcgta gaatcaatca
    12841 agtagcgact tgtattcaat cctttgattg gtaaaatgat tggttcagaa ccagtattga
    12901 tgaaaatccg ttcgccttca acaatcactt tttcaccatt attcgtcact tcgatttgat
    12961 ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc agttgcctca tcgctgatca
    13021 tatgataatt tttggcgcga agcatgcccg tcaatttttc tttccgagca acagcctctg
    13081 taaagtcgat tcctctttct ccattgaaaa tcaatgattt agaaggaata cagccgatgt
    13141 tgatacatgt tccgccatac atacgtgttg acttttcaac gactaaaacc gattcacctt
    13201 ttgaagctaa cgccttcgct aaagtcttac cgccctttcc aaaaccgatc acaatatttt
    13261 gatatttttc catcattttt ccctcgcttc ttttttacct accgattggt acaaaaatat
    13321 gataaccgat aattgtctat tctccaaatc atctgcttta tttgtcaaaa aaaaccagac
    13381 aaaaagcggt cctttcacgt ttttagtctg gttcgatttc ctttattctt ctaaacaatc
    13441 gttacttcta catcgatatt tcctctagtt gctttggagt aagggcagaa ttcatgcgca
    13501 tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat cgatatgcac ttctaatact
    13561 acaccgatct gatagccgcc atcatctaaa ttataaagag agacacgtgc ttttactgtg
    13621 gactctgctg tcattcctgc ttctttgatc atatgttgta ccgccccatt gaaacaagaa
    13681 gcataacctg ctgcaaataa ttgttctgga tttgttcctt ctgcatggat tcctggagga
    13741 gtgattttca tatgcatcga gccatttggt gcctcgactt ctccattgcg cccgccatgg
    13801 ttgatcattg ctgtttcgta tgcttttttc attgtactgc tcctttcgat tgaccatttg
    13861 ctacttggct ataattatag tgtatgacaa gtaaggcgct ttctacaaaa attctgctta
    13921 ttcgaatacc agaatgccgt cttctactat ttttccaaaa aacaaaaaaa tactactcgt
    13981 gaacaacagc gtcatatttg ttccaaaata aatagcaatc ttttctacat aatcttttat
    14041 atgattgatt ttaagataca tatataccag aaagttgacc ccataaacca atactgttaa
    14101 aaaaataacc atattcgtta ccattttttc ctccttttgc tggtctccct accagcactc
    14161 gatttaacca taccactttt tttgccgtcc aagcaactag cctttcaaaa agactggttt
    14221 attctgctgt taatgattca agatcagaaa aatgtccgaa taattttgct aattttacta
    14281 acgccaactt gttctttccg aattcatcat tttctatctg taatactagt aacggctcat
    14341 gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg ctcttctaga tcatatcgaa
    14401 taccttcttc attttccggg tcgaagttca tccctttttc ttcttccaac agtcgcttca
    14461 tttcttctaa tatcttgttt ccttgttcgc gtggttcatc gctgaacaat tttaatcgga
    14521 tttcttgtac ttctacgggc tgtttcaact ctttgatttg ttcatctaat gtgatacctt
    14581 ttctttttaa ttctggcaaa agcatcagga tctttgcgat gacatatgca ccatcatcca
    14641 gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc gatggctagc tgtgtatctt
    14701 tcccaagacg gttaagttct aatgctttat tgattacatt tcgatagccg gaaatataac
    14761 gaacttggat gcctcctttt tcttcaatga atctttgtag atgagaagaa gtaggggaat
    14821 tcgtcacgat tcttgttcct ggatgctctt ttaatacgat tgtacttaat actgcgatca
    14881 aattatttcg attgatcact gttccagaag cactgacaac tgcagaacga tccacatctg
    14941 tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac agcctgcttg atactttcca
    15001 tcgcttcttt attatcagga tttgggatat gattaggaaa tcggccatct ggttccaaaa
    15061 attgggaacc tgttgaatcc gcacctaagc tattcaatac tttttcagca aagaatccac
    15121 cagcaccatt tccggcatca acaatgattt tccaaccatt tagtggtttg ctttctagtg
    15181 ttccaatccc ctttttaatt tttgtgacta aatcttgcgc ataaggtgtc aaaaggtctg
    15241 ctttttctac gcttccagtt tgttcgcttt ttattttttg ctgctgtgat aggataaaag
    15301 caatatcttc tttttctgct cctccttctg aggtaaagaa ttttattcca ttataataat
    15361 agggaagatg gctagcagtc agcataaccg cagcatcgca cgagaactgt tcaaattgag
    15421 tggacataaa catagcaggc gtagtagcta attcgaaatc gatgacatgt atcccttgtt
    15481 ctttcagact ttggatcaac gcctctttca gtgctggtcc gcttatccta ctgtcatgac
    15541 caatcccaat cgtccatttt ttatttggat cggttgattt cttctttttc tttatccagt
    15601 ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt caagtttact tcatatcctt
    15661 caactggtaa tgcgattcct cgaatatccg atccattttg cagcgctttc atatctttca
    15721 tataaaagtc ctcctcattt attccctcat tttttccagc ttttctgcaa atcgctgtgc
    15781 ttcttcttct gagcggcaaa taataatgaa tgtatctaca ccggccatcg ttccgacaac
    15841 ttcagggtaa gccacttcat ctagccaatt cgttaccaca tcggcaccat ttcgttcagt
    15901 caatacgata accatgaact gaacagactg gatacgcaaa acctcgcgtc taattgctgt
    15961 acgcaaacgt tcttcattta aagaaacaga tggctggtta aataatgtat agcgtactat
    16021 tttgttttca ttttgacttt ttactaactt catttctcga atgtctcgag aaatcgttgc
    16081 ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc agctcttcct gtgtcccaat
    16141 aggttgttct gtaatgatct ggcggatcag tgcttgtcgc tgtttctttt tcatattaga
    16201 cacctctcaa tgttgattat agcaaaagat aagtaatatt tcttcatatt ttggtatttt
    16261 ttacttttat tttttgtttt cattcaggtt gctaaccttt ttctatttta acacgcaaaa
    16321 tcgtcttcat tttttcaggt gctgattttc ttggatcgct gatgtaaatc tctttgtgtt
    16381 ctttagaagc tctttcatag ccttcttttg cgatgaatgc ttctaatttc tcaaacgttg
    16441 ctggttcttc ggcatagggc ccgacgtgta agatttgggc acaaagacct tcttccatct
    16501 ttttgaatac tacttgttct ttcaagtctt ccggaagttt gctagaacgc tgaatggctt
    16561 cttgtgctat cgcttctgta acaaagtcag gctgtcggat catcaattga taagaaaaat
    16621 gttctttttt catcactggt tcatgcaaat acttttcttg cagtccccat tgtccttcca
    16681 gtggatagac agtatacatc tgataatcag gaatcagcca atttttttta ggcgacatgc
    16741 gtattgcata gctgactgca tatagacagg ctactcttcg tttaaagtct tcctgattcg
    16801 ggtcgccgat ccctctgatg ctaaagaatg attttttagg aacctctagt atttgcgggc
    16861 tttttttcat actatagctc ttgtctttac gccattcaat tttttccatt tttcttcctc
    16921 cgcgaaattc catttagtta tttattatca tacctctttt tgagtgactt ggctaatcgt
    16981 gttgacacta tttttcagtt tgtgctagaa taagtgaagt aaataaatcg tacagataac
    17041 acgtttcggc gtgtcgagag agaggcggtt tctattttta taatagaaat cactctcttt
    17101 ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca aaaacagctg cttttagtac
    17161 ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt attgatcgag acggccatga
    17221 atgtaacttt cccaactctt atcagacagt ttgatttaac gactgcacaa gtccaatggg
    17281 taacaacgat ttatcttttg atgatttcga tcattgttcc gctgtctact tatttgaatc
    17341 gcaattttag tctcaaaaaa ctctttttag cttcaaatct gcttttcttg gcaggtataa
    17401 tgatcgattt cttctctcct accttcatgg tcctattgtt tggtcgattg ctccaaggtg
    17461 cagcgacagg tatcgctctt ccgttgatgt tccatattat cttgacattt gcccccctca
    17521 atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac ggccattgct ccagcaattg
    17581 gccccactta tggaggcatt atgacttcac attttacatg gaatcatatc ttcctgtttc
    17641 ttgtgcccgt tttgtttctt tctttgatta tcggctggtt tgctattccc gagatggcag
    17701 tacaaaaaag tggttatttg gattggataa gcgttttagg aatttgtctt ttgtttagtg
    17761 gaagtctaac atttttgagt acgttgggaa cagtttccag ctggattgct ttaatcatcg
    17821 gcggtattgg ctggttcctt ttttactatc gaagtaaaaa agtggagcat ccactgatcc
    17881 gactgtctat tctttcaaat aaaacattcc gcttgttttt gattggcttc ttagtttatc
    17941 agtttttatt acttggtgta tcttttgtct tgccgaattt ccttcagatc gttcaaggaa
    18001 gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc agcggttggt gctttgcttt
    18061 ctcctttttc cgggaaaatg ctggatcaat atggtccgaa gaagccaatt tttgctgggc
    18121 tgattctttc tttgattggt tggctagcat tattcctact cataggacac gcttcagtgg
    18181 gtgtattgat tgcttgtcat gtgttttata tgatcggtat cggattatct tacagcaata
    18241 tgatgaccac cggaatgaat gcactgaatg aagaactaca aggagacggg aacgcaatgt
    18301 tcaatacctt gcagcagttt tctggcgctg tggctacttc attagtagcg gtcattatca
    18361 attacgtcca gcatcatact tcccataact atgaagtgtc tactacatta ggatcaaaag
    18421 cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt tgctcgattc gcctattatc
    18481 tgttctttgc taaaaaagcg tagattccat atacggcagg tgttgcatct gccgtttttt
    18541 tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg gaaaaaattg ccattatcgg
    18601 tggcgggatc attggtatga ccctagccag ttacttagat acatcaaaat tcaatgttgc
    18661 tttatttgat gaagggacag gacaggcaac taaagctagc gctgggatta tctccccttg
    18721 gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct aaagacggcg ctgctttttt
    18781 ccctaaactt ataaaagatt tccaacttga tcaaacgatt tacgcacaat cagggacact
    18841 tttgcttcga cctgaaaaag atttagctga attggctgtt cttgccgaga aacgaaaaga
    18901 agaagcgcca gaaattggaa acatcgaatg gctttcacct gaacagacat ctcaagcact
    18961 tccgttattg aaaccagcag tttcactgcg tatcagtggt ggtggtcgtc tggatggaaa
    19021 agcatattta gaacatcttg gtcagcgttt agcacaaaaa caaatggagc agatccaggc
    19081 aaaagtcact ttgcataaga agagcgattc ttggctcatc aagggaccgt ttggagaata
    19141 cacagctgat cgtgtaattt tgacacctgg cccggcactt aagcctttat tacaagaatt
    19201 aggctatgct gcagatatac gtccgcaaaa agggcagctc ttagcatttc agacatctta
    19261 taagatgagt caagactggc ctgttgctat gcttgacgga gaagctgatt tcattccttt
    19321 catggatgga aagatccttt taggtgctac ccatgaaaac gatcaaggct gggatttggc
    19381 tcctactagc gaagcttata agggattgac taagagtaca gaacagttct tgactgatcc
    19441 agatcagata aaaaactggc catacgagtt ccgtgtaggt acccgtgctt atacgtcaga
    19501 tttttcccca tttttcggcg tactgccaga tgatccaagc ttactggctg ctagcggtct
    19561 aggctcttct ggcttgacca caggtcctta tatcggctat ctattagcca acttcttaaa
    19621 tactggtcat tggcctacgg aagattatca aaaggacatc tccacctata ttacgaaaaa
    19681 tccatcatag aaaaacagct ttttcaagta aggatcccga gcgatattgc gcatggatta
    19741 cttcttgaaa aagctgttat tttagttcaa tttctgaatc aaattctcca aactttcttt
    19801 tgcttcatct ggcaagagat cttttcctat ctgagcttca atgaatcgta aacgaatatc
    19861 aagtcgttgt ttcaccagat ttttatagga cgtatcctca aaagaaacag gaaaatctgg
    19921 tataggtaaa tagtggatgt cttcaaatgg accaaacgac ttgaattctg ccgtttgttg
    19981 cacgattctt tcaacgattg acaatgtcgt ttcaggtttg atttttttat catcgaaata
    20041 tccagtattc aggacatagc aggctgtttg ctgtttcaaa aacaactctc gaaaagcttg
    20101 atagtcttcc ctaagaggat agacacggaa aggattagca aatggttcaa tgactagctc
    20161 atctagctgt ctttttgtag tgatattttc cgcgtctgat cgttttgttg ctaatgttgc
    20221 accaaaagac gctgctaaaa ctgggtcatt gatcttgata attggcggaa gcgaatcatc
    20281 tttcatgatc caaaaaacag catcgatctt ttcgctaaga tgatcgactc ggtcgggtgt
    20341 cgcataacgt gatttgactg tccgcccatt tccgtttcgt atatctcctg ttactaggac
    20401 tttttctcct ttacttgtac atgtcacgcc cacattctga caagtaagga aagaagaaat
    20461 ggccggatca tcagacggat agtcttgtgt tttatcaaag taagaaggtt ccaaggcagt
    20521 agcaaaacca ttttttcgag aaataacgaa agcatcgtca tgcaagatcg aaacagagtg
    20581 accttccgta tgttttgcta aggtaatcgt tgattttcca gaaccggaca acccaaatac
    20641 agccatcgta taggtctggt cattactagc atattgtttc atcccaccgt ggcaggcaat
    20701 aaagccattg cgatttccca atgcccaagc aagcgtcaaa gtcgcttttt ttaattctcc
    20761 aaagtaacgt aatcctagca cagccgctgt attatggaga gcatcaaaca aaatgagtcc
    20821 atttggaaat gtttcatgtg tccattcggg atcacaaaag atataaagat ctccctcatc
    20881 aaaggaaaca gacttttgat aagtctctaa gatcttcgaa ttcgctggct gaaaattcag
    20941 caaataagaa tacaaattgt tctcaaatcc ttctggaatc agcaaatgac tttgcacaga
    21001 aaattcttca gataggccaa cgattacttt tgtatggtaa aactggcgct tgcgactttg
    21061 atacacagct tctcttaaaa ttttctcgta tttgctttgt tccttgtcta agtcctcaaa
    21121 gatccgccga gcacttgctg ttcttcccac aatgcttcca tcattgaata ctagtgtcct
    21181 aacatcagct ggtaaatcta gttcttcagc atgtgagatt gttaaatctg tgatgatcgt
    21241 tcctggactc ccttttgcta gttgataagc tgtcttccta tcaaaaactt catgaacatt
    21301 attaccgtaa aatgtcgttt ctacaatcga tcgaatactg gataacagtg gatttccact
    21361 tgtgatttcg tttaattcga gactctcttt tgtactcatg gctcctcctc attccctaaa
    21421 caaaaaaacg ttttctttat tatacattat tcgagttttt atttgtactg ctgcgctgtg
    21481 ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa cttgaataaa caagtttctc
    21541 ggtttcatca ttttcctttt atcggcttag taccagccgt ttgctaacca gaatgtttta
    21601 gcagcatccc atgaaccata acgagatgtt acatattgtt ctgctacacg ttcttgattg
    21661 gcagctgaat agtcgccgtt taagtatgaa gcatctaatt gataacggcc gatgtaacgt
    21721 ccgttagtag ctgtgtatga accgcctgat tctttttgtg cgatccattc ttttgcagag
    21781 cttgaagttg gcgctgttgc agcagctggg gcagcaggag ctacttcaac tgtttcaaca
    21841 ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg tagatgctgt attttctgtt
    21901 gttttgtttt ctgttgttgc tgaagaatca tttgaagttg ggattgttaa ttgttcacca
    21961 acataaatgc ggttgatgtt tgcgatcgaa tttttttcag caattgcatc gatcattgag
    22021 ttgtctcctg caaatttttg tgagatctta gataatgaat cacctgattt tactgtataa
    22081 acttcatcag cgtgagcatt tgtagttcct acaaacattg cagtagcacc agcagcaaaa
    22141 gttgtaccta aaacaatttt cttaaatgaa ttcatattaa actccttttt ttctccgttt
    22201 tttattcttt cgacgctatt aactttatca tttagaaata tttcataggt aaaagaaaca
    22261 tgacatttaa tgacaaaata acgaattatt taatatttgt aacatttaaa caaatatttt
    22321 tttgatacaa aaccataaaa gccttgttgt ataagcaaag taatcgtttt tctatgtttt
    22381 tttatttttg aatgaaaaac gcagattctg taacattcac tgcaataatc cataaaaaaa
    22441 agcaaaaaaa tttttgattt ttatcaaatt ctcttaaaaa aatgtaaaaa atacaaaaaa
    22501 accagtaatt gttacaacaa ttactggaca aaaactttga ataatgttaa ctattttttt
    22561 attttaactt tctccgcaat aatttctggg catgttccaa ttcgtttata acgacttctt
    22621 ctgtttcttt agctaatatt tcatctaata catcaagcca tttttccggt tcttttgttc
    22681 ctagacgtcc aatagcccaa gctgctgttc ctctgatgac agggcggaca tctgttaaac
    22741 attctgaaat ctcaggcaat gccgttcgat cacctagatt agctaaagca atcaatgcgt
    22801 ttctctgcaa aggtttcttc ccacgccatg aaccagctaa atgcccaaat tgctgtttga
    22861 agtctttatt agacatcgaa agcattggct ttaattttgg atatatttcg tctacttttg
    22921 gttccatctc ttcatgaaaa tgaaaatctt ttccttgatt gtaagggcag actaactggc
    22981 agatatcaca tccataaatg acatttcgca tttttttacg atattcttcc ggcatcatcc
    23041 cttttgtctg agtttgataa gacaaacatc gctgggcatt cattcttccg tcaccaagca
    23101 atgcttttgt cggacaagca gtcacgcagc gcatacagtc accgcaacca aattctcctg
    23161 gtttatcagg ctcaaataca atattagtaa cgatctcacc aagatagaca aatgaaccga
    23221 actcttcagt gatcaacaat ccgtttcgtc caataaatcc taaaccagct cgctgtgcta
    23281 cagcgacatc gatcagttct ccagtatcta cttgcggagc tagacgccat tcctcttctt
    23341 tttgccattt ttctgctctt gtttggatga attcgatcaa tcggttcaaa cggtctctga
    23401 gtatatcatg gtaatcgatc ccccaagaag cacgagcaaa ctgcccgcgt ttttcatccc
    23461 taggcacttc ttcatggatc ctagtaggat acgcaagtgc aatggaaatg atcgatttcg
    23521 gctgatcaaa tgtcatttct ggataaagac gctcatcgat atttggatgt tcaaagccag
    23581 aagtatggcc agctgctttt tgttcttcca acgattcttt taatgaatca aaaggttccg
    23641 ccgtggtgaa cccgattttg tcaattccta atcgtttgct ttcttgtatg atttcttctt
    23701 ttaatgtcat ggtcttcctc gcttgctgtt aacggctgta taacccataa tatagttttt
    23761 cttttttcag ctggacaaac tggatctgct gatcggcaat cttcttttct tccatccatt
    23821 tttttcttgc agcaaatact tcccaataat ttgttgaaat taattttctt ttgttatcta
    23881 atgcattaaa aaaattcaat gaaactggtt ttgtacttaa cagttcatgg agccttacct
    23941 cttcccatgg cgaaaactca gacagatcgc gggaatacaa tacttcttca tagagatttt
    24001 tttgctgtgt gtacgtgaat gtttgcggga ttttcccagg cctagcaatt tctactagac
    24061 gattataaaa catattcatt aatttgctgt tggcaataaa ttgactttta taataggccg
    24121 actggatctt tttgattact ccttctgctt tgatgactga aaataatgca ggccatgctt
    24181 tgacggttat ttcgtcgttt tgtttaacta atacgccagg tgtgaagccg actggagaaa
    24241 ggaacacaaa ataaccgata ccggtatttt catctaaagg ctgaaattca tcaaacaacc
    24301 atttgcctat gtacagctct tcatctggtt ctgttgtctc attttgattt gctatgaata
    24361 atttagctaa ttctgcttca agaaattgaa ggtcttgatc cttgtcggta ttttcctgct
    24421 ggctgatcaa tcgaaagact tcttctttaa tcgtatattt atctttacgt gtaaaattca
    24481 tagccacacc ttccttcctt actctaacta taacggaatt tcttcaaaaa aggtatctgt
    24541 acgacgaaaa acagcataat tttttttcat atctcttata aaaaatgcaa ggtctgctcc
    24601 tcaagctaga ccttgcactt ttcagctttc ttttgtttgt gacaaacctt catggatcgt
    24661 ttcaatattc catttcatca tctgatagta agaatctcct tgttctcctt cttttgctat
    24721 ggaatcagtg aacagtttat catagatttt caaacccgtt tcttttgcta cccgttccat
    24781 gcttcttgag tctacactgg tttccacgaa taaaaccggt acttcttttg ctcttatctg
    24841 atcaatgatc gctttcattt gatcaggcgt accttgactt tctgtattga tctcccatat
    24901 ataagctgct ggcaaatcat atgctcgtga aaaatacttg aatgcccctt cacttgttac
    24961 taataatttt ttgttctctt cgatagaagc aaaactttcc ttcgcttgtg tatctaattc
    25021 ttttagtttt tccacatatg cttctgcatt ttttttatag agtgtcgcat tttctgcatc
    25081 tttttcagag aatatacgtg cgatttcctc cacatatttt attccgttag ataggtctag
    25141 ccaagcatgg ggatctgctt ttgtatgttc ttcaccgcta gttaaataaa gaggttctac
    25201 atttttgcta actgcaaaat aatcttttcc ttcttctttt ttagccgttt ccatcaagtt
    25261 atcgaaccag ctgttacctg tttcaagatt caaaccgttg tataaaataa catctgcatc
    25321 gcttgccttt ttgatgtctt ctggtagtac ttcatattca tgcggatctg ttccgactgg
    25381 tacgatactg tggatatcta tttgatctgt tccgacctct tttgccatgt ccgccaagat
    25441 cgaattggta gccacgactg acaacttttc tttacttcct tcatccgctt ggtcagaagc
    25501 ctgctttccg caaccggcta ataccaagaa actaactgcc aagattaaaa agagtgattt
    25561 ttttgttttc atctatctgc ttcctttctg aaataaaatt ccttttgttg gagaaaataa
    25621 aaaagcaagg ataaaaaatg cggtacatgc taacacgatc gtagctcctg atggccaatt
    25681 gtaactataa ctgaaaaaca caccgacaac tgcacttagc ataccgattc ctgcagctgt
    25741 aatgatcatt ttcagtaaat ggttcgttag caagtaagcc gtggcggctg gtgtgatcaa
    25801 catggcaatc accaagattg ttcctactgt ctgtaaactg actacagcca ctaatgtcaa
    25861 aaagaacatc aaaagataat ggatcagcga tgtgttcagc ccataagctt ttgccattgt
    25921 cggatcgaat gatgtgatct tcaattcttt ataaaagaaa aagacaaata tcaagacgat
    25981 tccactaacg agtgctgtca gcaacaaatc gctttcccta acagctaaga cattcccaaa
    26041 taaaatatga tacaaatctg tcgcactctg ggcatatgaa atcaagataa tccctaatgc
    26101 aaaaaaggag ctaaagacaa ttccaatcgc tgtatcattt ttcaatcggc tccgctgagt
    26161 cacgaaacca atcactgctg ctgttagcat cccaaaagca gtggctccaa agatgtaact
    26221 aaaaccaaac atataagaaa tggctacacc tggaagaact gcgtgagaga tagcgtctcc
    26281 catcaaagac atccctcgta aaataataaa agaaccgatg actccagaag caagtcctac
    26341 aagcatggaa gtgaccaaag cgtattgcag gaattgataa ttcattaatc catcaataaa
    26401 ggattggatc attttttctc tcctcctttt actaaatgtc tgatgatctc accataggcc
    26461 aattgaaggt tcttcgatgt aaagacttct tgtactggac cggaagcgat cagctgttta
    26521 tttaaaagaa taacctcatc gaaatattct tctactttat gaagatcatg atggacgatc
    26581 aaaatcgttt ttcctgaatt cttcaattcc tgcaagatgt caaagatctt tcgttcactt
    26641 aacgcatcaa tccctacgaa tggttcatct aaaaagatcc attctgctcc ttgggctaga
    26701 gctcttgcaa taaaaactct ctgaagctgt ccacccgata gttcgctgat ctgtcttttg
    26761 gcatattctt ccatccctac ttttttcaaa gcttgtcttg cacgttcttt ttcttttttc
    26821 ccaggtcttt gtccaattcg taaagatgga tatgttccta aaagtacaac gcctaataca
    26881 tcaattggaa aggaaagatc caattcactt cgctgttcca cgtaggctat ttttttacgg
    26941 actgagttta ccgactgtcc agaaaaggtg actttaccag tagtagctgg tatcaactct
    27001 agcaaagatt tcataaatgt tgattttcct gcaccgtttg gtccgataat accggtaatt
    27061 ttttgtggat ttatcgtcaa gttgatagtt ttcaaaacag ttttcccttg ataagcggct
    27121 gaaaggtctt gaattacaat ggccgtacgt tccaaatctt acactccttt ttaggtttac
    27181 ctaacttttt atttaaatat agtttaacta aaatggaaag cattttcaag tagaatgatg
    27241 aaaaagtttt tttcgaattt tcctcgcatt ttatgaaaat tttctgtaga atcacatcaa
    27301 gagttcaata aggctaacct aataatgaaa aacatgtgtt gaaacgtttg attatttttt
    27361 cttgttgact caacaacgaa aggagttttt gtatggaaga agaaaagtta actcgaagtc
    27421 ttagtgcccg tcatatccaa atgatcgcac taggtggaac gatcggtgtc ggtctgttta
    27481 tgggtgcctc ctcaaccata cgttggacag ggccctcagt gatgggagcc tatgcgattg
    27541 caggattctt tttgtatttg attatgcgtg ctttaggcga aatgctttac gttgatcctt
    27601 cgactggttc ttttgcaaat tacgcgagcg agtacatcca tcctgtggct ggctacttga
    27661 ccgcttggag taatattttt caatatattg tcgtaggaat aagtgaagtg attgctgtag
    27721 gatcttatat gaattattgg tggcctgatt tgccggcaat tattcccgga atcattgtcg
    27781 tcttgtttct tatgctggca aatcttattt ctgtcaaggc gtttggcgaa ttagaatttt
    27841 ggttttcgat catcaaagtc ataacgatca ttttgatgat cattgcaggt cttggggtaa
    27901 tcttatttgg atttggtaat catggtcagg cagtagggat ttcaaatctt tggaaaaatg
    27961 gtggattctt cactggaggc gtcaaaggat ttttctttgc attatcgatc gttgttgctt
    28021 cctatcaagg aatcgaactg atcggaatga ctgctggtga agcagagaat cctaaaaaga
    28081 cgatcattga agctgtccag tccactatcg gtcgaatctt gattttttat attggtgcta
    28141 tttttatcat tgtgagtatc tatccgtgga atcaattgag tgagattggt tcaccttttg
    28201 tacaaacttt ttctaaagtc ggcattacat ttgctgcagg actgattaat tttgtcgtga
    28261 ttacagcggc attatcaggt tgcaattcag ggattttcag cgcaagtcgt atggtctata
    28321 cacttgccat aaatggaaag atgtctaaaa aatttctgaa actatctcgt cacggtgcgc
    28381 ctttttatcc tgtcgtagca atctcaggtg gaatcttaat tggtctgatc ttaaattatc
    28441 ttctaccaat gttcatcaac cagtcagaaa atattttcgt tttcgtatac agctcaagta
    28501 ttttgcctgg tatggttcct tgggtagtga ttttgatcag cgagattcgc ttcaaaaaga
    28561 agcacgcaga taaaatgcat gatcatcctt ttaaaatgcc actatctcct tatacgaatt
    28621 atgtgacact tgctttttta gctatcgtat tgattttcat gtttatcaat cctgaaacga
    28681 gaatctcatt gattattgga ctcgttttct tgctttacat gacgatccat tactttgtac
    28741 gcgaaaaaag aaccggaaaa taaaaaaaga acgaagcttc accttagtgg aagcttcgtt
    28801 cttttttatt gcttttatca attcaatttt agcgtctcac cgggagtcag gaaataattc
    28861 tccatgctca tcccattcag cgacaagatc gtttctactg gtacgccagt tcgttcggct
    28921 atttgatttg gaccttctcc ttccaaaact acggttgtcg cagcctgtga agtcgcgctg
    28981 ttggtattag tttcagagga atctgttgtg tcttgtgttg ttcgagcttg catttcagaa
    29041 gcttctaccg actctgctgt ctcagaatca gaagatgttg tgtcttgtgt gcttgttgac
    29101 tctgcttgaa gagaggcgct agtctgcaaa taaatatcgg aaatcttagc gacatgattc
    29161 gtataattag ccgataattg attgatcaaa gtatctaagt ctacaccgtc tttattttct
    29221 gctgcctgat acgctgcacg taattttctg atcccttcgt cggagattgc ttgaagctca
    29281 cgtgcttttt ggttagcaac tgctgataaa ccagctattc cctcttgatt tccctgaatt
    29341 tctgtctggt attcttcaat caatgcaggc gtcttttctt cgatcttttt cgaataatca
    29401 tttacgattg cttgaagttg ctctggcaaa ccatttgctg ttttttcttc agaagtttgt
    29461 gtctcttttg ttttagttga tgactcactt gtttgtttcg tctctgtttt ctttgtcgtt
    29521 gtttccgtta tggtcgtact tttcgcttct gacgaatttt ttccatgaaa atatagaccc
    29581 gcaactcctc ctgctatgat caaaaacacg gcaactgctg ccccaatcat gatcatccgc
    29641 ttacgagctg cctgtttttg ctgtcttctt cgggtcatcc gaacattttc tccccactca
    29701 ttttcatcat acattttttt caccaccaat ttttatgtat aatagcgtta tcaaacgctt
    29761 catattctta ttgtagcaaa taattgtcgc tgtgcacatt tttatctttg ttatttataa
    29821 gaaaatttca ccagttgaga tgattccaag attgtccgtc gatttctcct tgcagctgca
    29881 ttgttcgttc gtctatctca gccatcagtt tctttatttc atataagagc gcacggtctt
    29941 gatattctga tttttcgctt aattcatcta attgttggat cagccaatca tttgtcataa
    30001 tgaagttcct ttcatttctt gcagtgtaaa ggtcaagtct tcgatctgct cttttgcctc
    30061 agtcagtgct tctacgatat atgcttgttt ttcagattct tcttcctgca ttttctgatt
    30121 ttcctgtact ctttgaataa accagtcaat ccgctgagtc agcgatggtt tgccttccaa
    30181 tgagagatac tgctgataag caattagctt ctcccaacgt ttttcctggg ataaatacat
    30241 aaattgtgct aaaacgaatg gcggaagata ggtcatatgg aatttatttg cgattcgctg
    30301 atacggcctc aaaaattcgg aaatcgtata ttcttctttt cctccagctt ggtattcttt
    30361 ttccgccaca cctgttgtca atacgattcc tagttctttc ccttttaata catgtgctgc
    30421 tcgttccaag acattatctt gccaaatttt taaatgagca ggagaactat accagtaaaa
    30481 cggaaactgg aaaatgatcc gatcatgctg ggccaagagc tgtagttcag tctctatatc
    30541 tatttttccg tctggataac ttgattccaa atgatgccaa gtagctggta aaccagcaga
    30601 agcttctttg aaaaattgct gatttaaaga ttcttcgatc gttggatggc taatgatgat
    30661 aagtgtacgc atcccttttc ctccttttta gtcttcgctt tacagtgtag cataaccgcc
    30721 acaagtttct tttgccgatg cttgtcattt taaccagact aatcaggttg tccaagaaac
    30781 tgcggtttgt ttttccaact gttttttcaa ggtatccaaa gcaatattct taaaattttc
    30841 gtttttcata cgtttacgat agttcgtagc cgtttctccg gtactctttt tgaacattcg
    30901 gctaaatacg actagactat tgaaccctac ttgataggca atactttcca cggattcatc
    30961 cgtcagagaa agaagaaacg cccctcgatt gatccgtacc ccgatcaaat attccttgat
    31021 actggtaccc atatgatttt tgaatactcg cgacaaatgg ctccgattga ccgctaaatg
    31081 ctgggcgatt tcctcaacag acaatttttc agcataatgt gcctcgatgt atttgactgt
    31141 ttcaacagct aaagaggaat gaaccttttg tacaccaccg ctgacctttc cgccatcttt
    31201 taaaaggact gataaaaaac ggtaggttaa tgctgttaac tgtaattcat tttccaaggt
    31261 ttcttccgta tctttcatgc attctaaaat aatgtcgata tatttttgta tgtcagaaga
    31321 gaccatcgtg tattggtctt ctctgaaaag cgactgccga atgatttctt ctgcagcact
    31381 cccaccaaat gaaagccagc aatacatcca cggatcttct ccatctgata agtaaaacgt
    31441 cgagtctcct ggacgaatca agaacagatc accttttttc aattggaatt gttgattttt
    31501 cacatgataa gatcctttgc cttccatcac gacatgcagg atgtaatcat tgcgaatagc
    31561 aggaccaaaa gaatgaaacg gcatcgtttt agagaaacca caaaaatcaa aatacaattc
    31621 tttttctact tgttgatgtt ccttttttaa aaaaatcgat tgctccacgt ctatccctcc
    31681 ttactccttt agtataatgc aacaaaaagt taaataaaag tcacagaaat atattaaaaa
    31741 ggaagcgatt tcttgttaag tttaagtcat taaaagatct ttgttattcg ttgtaaccgg
    31801 tttacttagt attatatatt gaacaggaga ggaatacaga ttatgaaaag caaaatgatc
    31861 caacgtgtgt cctacgcttt aggcgctttt ggacacgaca cattctatgt tacattaagc
    31921 acttatttta tggtattcgt gacaagccag atgttcgcag gaacagataa ggcaacgaat
    31981 gcaaaaatga ttgctacagt tacaagctta gttgtaggta tccgcttagt tgaaattatt
    32041 tttgatcctt tgattggtgg catcatcgat aatactcgta caaaatacgg taaattcaaa
    32101 ccatggttag ttatcggcgg cgttgttagt tccgtactct tggtagcaat ctttaccaat
    32161 ttctttggct tagctactca taatacgact ttatttatca tattatttgc aatcgttttc
    32221 atcttattgg atagttttta ttcatttaag gatatcgctt tttggtcaat gattccagct
    32281 ttgagtacgg aaacaaagga acgtgaaaaa tttgctacgt ttgctcgttt cggctcttct
    32341 ctaggtgcaa atggtactac tctagtcgtt gtccctatcg ttactttctt tacctatctt
    32401 gttactcata aacatattga aggcgcttca ggctggttct ggttcggtgt gattgctgct
    32461 gttatttcag gcggtactgc gattatcact gccttgggca caaaagaagt cgatactgcg
    32521 atccgtcagc aaaatgaaaa agctgggatc aaagatgtgt tcaaagcagt tgctcaaaat
    32581 gatcagttga tgtggcttgc ccttgcttat atcgcttatg cgattgccaa tgcagcaaca
    32641 actgccgtat tattctactt cttcaaatat gtacttggcc aaccaactga attttggatc
    32701 gttggtctag ttgctgcctt acttggttta gtagctgttc cactattccc aatgcttaca
    32761 cgcgtcatct ctcgtcgcta cgtttacctt ggagggatcg caatgatgat ttcttcttac
    32821 tttttcttta cgatcgcagg ttcaagtttg ccaatcgtta tcatcgggct agtattgttc
    32881 tacttccctc aacaattgat tttcttatcc gctttgatga cgatcacaga ctctgttgaa
    32941 tatggtcaat ggaaaaatgg tatccgtaac gaagctgtta ctctatcaat tcgcccttta
    33001 ctagataaaa tcgctggtgc tttgtctaac gggatcgtag ggtttgtcgc tatcgcttct
    33061 gggatgactg gtaatgctac tgctgctgat atcactgctc atggcgtaac gactttcaaa
    33121 gcttatgcat tctatgctcc tgctgctttg atgattttct ccgctattat ttttgcttgg
    33181 aagattacat tgacagagaa aaaacatgca caaatcgttg agaaattgga aaacaaattg
    33241 gctccagctg atacaatgga agatgaactt cacgatgcag tagatccttc ccattaagaa
    33301 aggatcttga aaatgaagat tacagaaaaa gattttggcc aaggttatca cttgataacc
    33361 ttggagaaca aaaacaagct agcactttct atctctgatt taggtgctag aatcgtcagt
    33421 ttaaaatcaa atgatcgaga attagttttg ggatttgaca ccgctgaaga atatatcgaa
    33481 aaagatccgt atatcggtgc ttctatcggt cgcaccgcag ggagaatcga aaatggccgc
    33541 ttttctctca atggaaaaac ttaccaatta gccacagatc caaaaactgg acataatctg
    33601 catggtggtg ctcctggatt tgaattaaaa aaatggtctt atgtcatttt gaatggcgaa
    33661 aatgaagctt ctgtcatttt tactactaca agtcccgacg gagaacatgg ttttccaggt
    33721 acaatggatg tggaaatccg ctatactttg acaaaagata atatctggcg agtgacctct
    33781 cgcggcacca gtgaccaaga tactttattc aatccgacaa atcatgttta cttcaacttg
    33841 acgggagatg cttctcagtc aatcgatcaa catgagcttt ggctcaatag cgaagcttat
    33901 gctcctttgc gaacagatag tattcctata ggggtaaaag aaaatgctgc aggaagtgct
    33961 ttcgattttc aaatacctaa aaaattagcg tccgtttttg ctagtgattt ggatcaaaaa
    34021 aatcttgttg acggaatcga tcatccattt ttcttaaaag aaaccggctt gggtaaagaa
    34081 gccgctcgct tgactagccc cgataaaaga atccaagtag atattgctac agatgcttct
    34141 agtgtggtta ttttcactgc aaatttcggc acagaaacac cagaaatgcg caaccgaaaa
    34201 ctggcacatc acggtggcat cacatttgag actcaaacag ctccaggtgc agagcgcttc
    34261 tcagcatttg gttcgattca tttaaaagcc ggatcagtat ttgaaacagt aacagaattc
    34321 aaaatcaaaa caagaaagga ataactcatc atggaaatca aaagaagctt aaacacaaaa
    34381 tttgccgaaa cattcggacc taaaaaaact acacaatact tctcaccagg acgcatcaat
    34441 ttgattggtg aacacacaga ttacaatgga ggccatgtct tccccgcttc catcacttat
    34501 ggaacgcaag gtgtagcagc acctcgagac gataataaaa tattagttta ttccacaaac
    34561 tttgacgata tcggtgtcat tagttttact ttagatgagc tagaatatga taaaaaagca
    34621 aactgggcaa attacgtaaa aggaatggtc cttaaattaa aagaagctgg acactcaatc
    34681 gatcatgggt ttgagttatt agtggaagga acaatcccta atggtgcagg actttccagt
    34741 agcgcttcat tagaattatt agtaggtgtt gtattagaag atctgtttga tttaaaaaca
    34801 gatcgcttgg aactagtcca aacaggtaaa aaagtagaaa atgaattcat cggtgtcaat
    34861 tcaggtatca tggatcagtt tgccatcggc ttcggtgagc tagatcatgc aatcttatta
    34921 gatacaaata cgctgaaata cgaaatggtt ccagtgaaac tagatggtta tgcaatcgtc
    34981 atcatgaata cgaataagcg gcgggaattg gcagattcaa aatacaatga acgccgtagc
    35041 gaatgcgaag aagccttggc acgtttgcaa aaaactttgc ctatctcttc tttaggagac
    35101 ttagatgaag aagaatttga atcaactatt gaccaaatcg gcgatgatac attgatacgc
    35161 agagctcgcc atgccgtttc tgaaaatcag cggactttga aggcaaaagc tgaattagaa
    35221 gctgggaact tagaagcatt tggtcaactg ttaaatgatt ctcaccactc tcttcgctat
    35281 gattatgaag taactgggat cgaattagat acactagtag atgctgctca aaaacaagaa
    35341 ggtgtcttag gtgcacgaat gacaggagct ggatttggtg gctgtgcgat tgctcttgtc
    35401 aaagaagaaa atatacctgc cttcaaaaac aatgtctatg atgaatacat gaaagttgtc
    35461 ggctatgctc cggaatttta tgttgcacac attggaaatg ggacaacaaa gatcgatcca
    35521 gaagctgttc atgtttgata aaaaaatcaa cgatctcagc taaaaacaaa tagataaaaa
    35581 taacctctca gcaatttcca aatgtgagcc ttttgctcgt atttgaaaat tgctgttttt
    35641 tattgacacg gaaagagaac gtgtgttcta attgtgttga agaggtgatt gttatgtggg
    35701 cgaatcaaaa tccggtattt gtttatgaaa aagaaccttc aagagatatt ttatgtattg
    35761 actgcaaatc tttttacgcc tccgtagagt gcgttgaaaa ggatcttgat ccccttacaa
    35821 ctaaattagt agtcatgtcc tatccgtccg attcgacaga aacacggggt agcggattaa
    35881 tcttggcttc cagtcctgca gcaaaaaaag cttacggcat caccaatatc agccgtgcca
    35941 gagatcttcc cttcccctat ccaagtgatt tatatattgt tgccccaagg atggcctatt
    36001 atatggaaaa gaacagagaa atcaattcta tctataaaaa atatgcagac gaagcgaacc
    36061 atcatgtcta ttccgtcgat gaaagcttcc ttgatgtaac aaatagcatg gcactctttg
    36121 gtaaaacaaa tgcgtacgac atggctaaac tgatccataa agatgtgttt gagcagaccg
    36181 gaatctacac gactatagga attggcgata acccgctact tgctaaacta gccttagaca
    36241 atggctcaaa agacggaaag caaatgtttg ctgaatggcg ttatgaagat gtaccagaaa
    36301 agttatggaa aatctcagat atcactgact tttggggaat cggcaaacga acagcaaaac
    36361 gtctgaaaca aatgggtata cattctgttt atgatttagc tcacgcggat tattatcact
    36421 tgaaaagcaa gctaggggtc atcggtactc agctttatgc ccatgcttgg ggcatcgatc
    36481 gaagtttttt gggacaagaa tatacaccaa aatcaaaaag tatcggaaat agtcaaatcc
    36541 tagatagaga ttatacaaga cgagaagaaa tcgagatcgt tatcaaagaa atggccgacc
    36601 aagtcggcac tcgcttgaga cgatctgcag caaaaacaca agtagtcagc ttatgggtag
    36661 gatactcttt aggctatctt gatccaaatg gcaagaccgg gtttcaccag caaatgaaaa
    36721 tcgaagcgac aaatgctagc agagatctag cagatgcttt attgaccatt ttccaccagc
    36781 attaccacta ccaggatatt cggcgtgtag gcgtgaactg ttccgacctc gtatacgcga
    36841 caggtttaca gcttaacctt tttgaagatc ctgaaaagca gataaatgaa gcgaaagttg
    36901 attttgttgt cgatacgatt agaaaaaaat acggctttca atcaatcatc catgcgcata
    36961 gtctcttgca aggaggacgc gccatcgcac gtagttctct cgttggcggg catgcaggtg
    37021 gtatgagtgg attagaaggt ggaaaagatg agacggacag caaaaaaatt cattgactat
    37081 aacgagtatc gagatcggcc atttggatta aaatggggga ctgcttttgc gatggaagaa
    37141 ctagtcaacg gtatcaaaaa aatgaagaag aagcacgcaa acagcctctt cctaaacaac
    37201 aaatgacatt gcaagaaatc gatagtatac tgacagaagc ctttttatct cgtaaagcag
    37261 taatcattca gctcaattta aaagatgaat ttggaagact cttggatgag gtcgaagggc
    37321 acttcattgg tgaagcctat gaagattact ttatgattga ccatcagccg atattgtggg
    37381 aagacgtacg gtatattgag ttaaaggaaa caaaaaaatg gtttgtgaca tgagaggttg
    37441 tgacagaagt gatcaacttc aaaaaataag aaaggatttt tcgaaaattg cttcttaaat
    37501 ttttgtggaa ttttggctta tttttcgaag aagcaacttc tggaacaacg tatattagat
    37561 atcagaggtt gtgaaaaagt cgcttagttt tgagtaacaa gccaaggcgc tgatttcaca
    37621 acctctgtct atctatccat ctattcggta agattcatgc atgacgtctt ctttatttag
    37681 tatgtagagc cctttttctt ctgaccattc aacataaatc agctgtttta catctgcata
    37741 gccttctttc ttcaagttct ccataagcca tgcttcagtc aattgattgg actgaagcac
    37801 tttatgctga atttgccctt catctacaag caggatcgat aaagctttgt cttcatctga
    37861 ttttttcaat acactcattt gaccggcatt ctctaggata acatgtttgg cattttcaag
    37921 aaaaaagcat tcttgctgtc ttaagattgc tcgcaactgc tccatctcga tatggttatt
    37981 tgtcaactct gtcatgttga tgacgccgtc tttgattaga acagatggct cgcctttgac
    38041 ccaacgattg accttttcat ttttttggac gatacgttcg ataccataaa tcattactgc
    38101 ccaaagcgca atggcaaata ggacatgccc tacatggaca ttgtcatcat aggttgattc
    38161 ttctaagatc ccacctaaaa caagcgtata gactaaatca aacggtgtga tttctgacat
    38221 ggatttcttc ccaagcaaac gtaccactaa taataaaccg atcaacccga acaccagttt
    38281 cattgtaatt gaaaaaatca ttttttctcc ccctaactat ctgttaatta aattatatat
    38341 gctttctcac gaacacgcat ggaatataca aggaagaaat gtatcctttt actaaaaaaa
    38401 ggactgtgac actgtagtga cccccaaaag ttagactttt ttggagtgga gaaatccact
    38461 cctttttttt atgctctaaa atgatatttt tacacaaccg ttacggttgt tttacgaaat
    38521 tggacg
//
LOCUS       DACCWK010000019        36249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCWK010000019 DACCWK010000000
VERSION     DACCWK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.019"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            160..840
                     /locus_tag="IVW65_000889"
     CDS             160..840
                     /locus_tag="IVW65_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7899808.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IVW65_000890"
     CDS             930..1964
                     /locus_tag="IVW65_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7899809.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IVW65_000891"
     CDS             1978..2313
                     /locus_tag="IVW65_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899810.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IVW65_000892"
     CDS             2297..4777
                     /locus_tag="IVW65_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899811.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IVW65_000893"
     CDS             4833..6392
                     /locus_tag="IVW65_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7899812.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IVW65_000894"
     CDS             6389..7753
                     /locus_tag="IVW65_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7899813.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IVW65_000895"
     CDS             7924..8994
                     /locus_tag="IVW65_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899814.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IVW65_000896"
     CDS             9009..9677
                     /locus_tag="IVW65_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899815.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IVW65_000897"
     CDS             9697..10275
                     /locus_tag="IVW65_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7899816.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IVW65_000898"
     CDS             10676..11587
                     /locus_tag="IVW65_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7899817.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IVW65_000899"
     CDS             complement(11965..12882)
                     /locus_tag="IVW65_000899"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7899818.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IVW65_000900"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IVW65_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7899819.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IVW65_000901"
     CDS             13579..14127
                     /locus_tag="IVW65_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7899820.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IVW65_000902"
     CDS             14242..16602
                     /locus_tag="IVW65_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7899821.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IVW65_000903"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IVW65_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7899822.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IVW65_000904"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IVW65_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7899823.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IVW65_000905"
     CDS             19108..20007
                     /locus_tag="IVW65_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899824.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IVW65_000906"
     CDS             20000..21262
                     /locus_tag="IVW65_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899825.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IVW65_000907"
     CDS             21423..23321
                     /locus_tag="IVW65_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7899826.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24369)
                     /locus_tag="IVW65_000908"
                     /pseudo
     CDS             complement(23651..24369)
                     /locus_tag="IVW65_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
     gene            24706..25038
                     /locus_tag="IVW65_000909"
     CDS             24706..25038
                     /locus_tag="IVW65_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899827.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25025..27028
                     /locus_tag="IVW65_000910"
     CDS             25025..27028
                     /locus_tag="IVW65_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7899828.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27047..27517
                     /locus_tag="IVW65_000911"
     CDS             27047..27517
                     /locus_tag="IVW65_000911"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7899829.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27538..28122
                     /locus_tag="IVW65_000912"
     CDS             27538..28122
                     /locus_tag="IVW65_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7899830.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28137..29123
                     /locus_tag="IVW65_000913"
     CDS             28137..29123
                     /locus_tag="IVW65_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7899831.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29113..29424
                     /locus_tag="IVW65_000914"
     CDS             29113..29424
                     /locus_tag="IVW65_000914"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7899832.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29446..31227
                     /locus_tag="IVW65_000915"
     CDS             29446..31227
                     /locus_tag="IVW65_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7899833.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31220..32596
                     /locus_tag="IVW65_000916"
     CDS             31220..32596
                     /locus_tag="IVW65_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7899834.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32603..33238
                     /locus_tag="IVW65_000917"
     CDS             32603..33238
                     /locus_tag="IVW65_000917"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7899835.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33268..34623
                     /locus_tag="IVW65_000918"
     CDS             33268..34623
                     /locus_tag="IVW65_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7899836.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34815..36209)
                     /locus_tag="IVW65_000919"
     CDS             complement(34815..36209)
                     /locus_tag="IVW65_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7899837.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT        11968 a         6222 c         7722 g        10337 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gttccgttag
    24121 ctttggatga ggatcttcca tgtaaagttg tggactgaca tcttgaccac ctgcaagaat
    24181 aagtttgtcg atttgtccaa tcagttgagg agctgaatct ggagacatga ctggcagaat
    24241 aagaggcaat ccttgagcat ctaaaactgc atccacgaat ccttgcggtg tatagctcac
    24301 ttgatttcct tggaaagtat ctgttgctcg aatcagttga tttccggcaa tgccgatgat
    24361 tggtttcatt tgtattcccc ctaaagtaga cttattgtaa caaggattta aaaaaaatta
    24421 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    24481 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    24541 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    24601 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    24661 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    24721 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    24781 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    24841 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    24901 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    24961 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    25021 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    25081 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    25141 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    25201 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    25261 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    25321 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    25381 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    25441 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    25501 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    25561 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    25621 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    25681 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    25741 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    25801 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    25861 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    25921 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    25981 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    26041 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    26101 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    26161 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    26221 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    26281 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    26341 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    26401 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    26461 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    26521 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    26581 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    26641 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    26701 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    26761 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    26821 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    26881 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    26941 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    27001 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    27061 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    27121 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    27181 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca
    27241 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    27301 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    27361 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    27421 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    27481 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    27541 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    27601 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    27661 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    27721 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    27781 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    27841 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    27901 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    27961 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    28021 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    28081 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    28141 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    28201 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    28261 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    28321 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    28381 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    28441 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    28501 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    28561 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    28621 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    28681 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    28741 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    28801 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    28861 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    28921 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    28981 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    29041 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    29101 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    29161 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    29221 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    29281 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    29341 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    29401 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    29461 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    29521 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    29581 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    29641 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    29701 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    29761 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    29821 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    29881 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    29941 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    30001 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    30061 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    30121 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    30181 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    30241 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    30301 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    30361 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    30421 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    30481 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    30541 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    30601 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    30661 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    30721 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    30781 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    30841 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    30901 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    30961 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    31021 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    31081 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    31141 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    31201 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    31261 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    31321 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    31381 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    31441 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    31501 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    31561 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    31621 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    31681 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    31741 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    31801 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    31861 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    31921 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    31981 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    32041 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    32101 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    32161 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    32221 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    32281 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    32341 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    32401 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    32461 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    32521 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    32581 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    32641 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    32701 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    32761 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    32821 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    32881 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    32941 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    33001 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    33061 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    33121 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    33181 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    33241 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    33301 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    33361 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    33421 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    33481 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    33541 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    33601 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    33661 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    33721 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    33781 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    33841 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    33901 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    33961 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    34021 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    34081 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    34141 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    34201 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    34261 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    34321 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    34381 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    34441 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    34501 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    34561 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    34621 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    34681 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    34741 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    34801 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    34861 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    34921 gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    34981 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    35041 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    35101 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    35161 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    35221 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    35281 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    35341 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    35401 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    35461 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    35521 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    35581 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    35641 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    35701 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    35761 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    35821 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    35881 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    35941 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    36001 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    36061 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    36121 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    36181 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    36241 aattttacc
//
LOCUS       DACCWK010000020        34590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCWK010000020 DACCWK010000000
VERSION     DACCWK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.020"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            212..604
                     /locus_tag="IVW65_000920"
     CDS             212..604
                     /locus_tag="IVW65_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7899838.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IVW65_000921"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IVW65_000921"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7899839.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IVW65_000922"
     CDS             3165..4775
                     /locus_tag="IVW65_000922"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7899840.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IVW65_000923"
     CDS             5067..5936
                     /locus_tag="IVW65_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7899841.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IVW65_000924"
     CDS             6101..7372
                     /locus_tag="IVW65_000924"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7899842.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IVW65_000925"
     CDS             7369..8664
                     /locus_tag="IVW65_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7899843.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IVW65_000926"
     CDS             8763..9026
                     /locus_tag="IVW65_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7899844.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IVW65_000927"
     CDS             9471..9920
                     /locus_tag="IVW65_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7899845.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IVW65_000928"
     CDS             9977..11395
                     /locus_tag="IVW65_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7899846.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IVW65_000929"
     CDS             11395..12255
                     /locus_tag="IVW65_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7899847.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IVW65_000930"
     CDS             12429..13202
                     /locus_tag="IVW65_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7899848.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IVW65_000931"
     CDS             13349..13714
                     /locus_tag="IVW65_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7899849.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IVW65_000932"
     CDS             complement(13862..15070)
                     /locus_tag="IVW65_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7899850.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IVW65_000933"
     CDS             complement(15089..15367)
                     /locus_tag="IVW65_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899851.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IVW65_000934"
     CDS             15560..16216
                     /locus_tag="IVW65_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7899852.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IVW65_000935"
     CDS             complement(16658..17455)
                     /locus_tag="IVW65_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7899853.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IVW65_000936"
     CDS             complement(17452..18636)
                     /locus_tag="IVW65_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7899854.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IVW65_000937"
     CDS             complement(18661..19908)
                     /locus_tag="IVW65_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7899855.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IVW65_000938"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IVW65_000938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7899856.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IVW65_000939"
     CDS             21888..22202
                     /locus_tag="IVW65_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7899857.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IVW65_000940"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IVW65_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7899858.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IVW65_000941"
     CDS             22887..23531
                     /locus_tag="IVW65_000941"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7899859.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IVW65_000942"
     CDS             23546..23875
                     /locus_tag="IVW65_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899860.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IVW65_000943"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IVW65_000943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7899861.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IVW65_000944"
     CDS             24863..25687
                     /locus_tag="IVW65_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7899862.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IVW65_000945"
     CDS             25680..26027
                     /locus_tag="IVW65_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7899863.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IVW65_000946"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IVW65_000946"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7899864.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IVW65_000947"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IVW65_000947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7899865.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IVW65_000948"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IVW65_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7899866.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IVW65_000949"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IVW65_000949"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7899867.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IVW65_000950"
     CDS             31684..32451
                     /locus_tag="IVW65_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899868.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IVW65_000951"
     CDS             32441..34483
                     /locus_tag="IVW65_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899869.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11119 a         6130 c         7259 g        10082 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat
//
LOCUS       DACCWK010000021        32533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCWK010000021 DACCWK010000000
VERSION     DACCWK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.021"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            131..505
                     /locus_tag="IVW65_000952"
     CDS             131..505
                     /locus_tag="IVW65_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7899870.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVW65_000953"
     CDS             complement(619..1248)
                     /locus_tag="IVW65_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7899871.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVW65_000954"
     tRNA            complement(1382..1454)
                     /locus_tag="IVW65_000954"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVW65_000955"
     tRNA            1668..1741
                     /locus_tag="IVW65_000955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IVW65_000956"
     CDS             1696..1845
                     /locus_tag="IVW65_000956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899872.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IVW65_000957"
     CDS             2338..3666
                     /locus_tag="IVW65_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7899873.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IVW65_000958"
     CDS             3868..4701
                     /locus_tag="IVW65_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7899874.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IVW65_000959"
     CDS             4717..5454
                     /locus_tag="IVW65_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7899875.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IVW65_000960"
     CDS             complement(5609..6577)
                     /locus_tag="IVW65_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7899876.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IVW65_000961"
     CDS             6809..7642
                     /locus_tag="IVW65_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7899877.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IVW65_000962"
     CDS             7676..8515
                     /locus_tag="IVW65_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7899878.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IVW65_000963"
     CDS             8541..9101
                     /locus_tag="IVW65_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7899879.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IVW65_000964"
     CDS             9300..11021
                     /locus_tag="IVW65_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7899880.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IVW65_000965"
     CDS             11186..12328
                     /locus_tag="IVW65_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7899881.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IVW65_000966"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IVW65_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7899882.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IVW65_000967"
     CDS             14128..14775
                     /locus_tag="IVW65_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7899883.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IVW65_000968"
     CDS             15168..17813
                     /locus_tag="IVW65_000968"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7899884.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IVW65_000969"
     CDS             18123..19436
                     /locus_tag="IVW65_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7899885.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IVW65_000970"
     CDS             19426..20079
                     /locus_tag="IVW65_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7899886.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /locus_tag="IVW65_000971"
     CDS             20134..20826
                     /locus_tag="IVW65_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7899887.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="IVW65_000972"
     CDS             20976..21176
                     /locus_tag="IVW65_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7899888.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="IVW65_000973"
     CDS             complement(21430..23109)
                     /locus_tag="IVW65_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7899889.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="IVW65_000974"
     CDS             complement(23111..23323)
                     /locus_tag="IVW65_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7899890.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="IVW65_000975"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="IVW65_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7899891.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="IVW65_000976"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="IVW65_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7899892.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="IVW65_000977"
     CDS             27298..27495
                     /locus_tag="IVW65_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899893.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="IVW65_000978"
     CDS             27648..28133
                     /locus_tag="IVW65_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7899894.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IVW65_000979"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IVW65_000979"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7899895.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="IVW65_000980"
     CDS             29010..29537
                     /locus_tag="IVW65_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7899896.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="IVW65_000981"
     CDS             29628..30365
                     /locus_tag="IVW65_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7899897.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="IVW65_000982"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="IVW65_000982"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7899898.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="IVW65_000983"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="IVW65_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7899899.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5521 c         6874 g         9366 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgtt gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DACCWK010000022        32255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCWK010000022 DACCWK010000000
VERSION     DACCWK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.022"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(99..1229)
                     /locus_tag="IVW65_000984"
     CDS             complement(99..1229)
                     /locus_tag="IVW65_000984"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7899900.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVW65_000985"
     CDS             complement(1878..3080)
                     /locus_tag="IVW65_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7899901.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVW65_000986"
     CDS             complement(3140..3343)
                     /locus_tag="IVW65_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7899902.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVW65_000987"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVW65_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVW65_000988"
     CDS             4880..5692
                     /locus_tag="IVW65_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7899903.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IVW65_000989"
     CDS             complement(6716..6868)
                     /locus_tag="IVW65_000989"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899904.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVW65_000990"
     CDS             complement(7059..7937)
                     /locus_tag="IVW65_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7899905.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVW65_000991"
     CDS             complement(7976..9094)
                     /locus_tag="IVW65_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7899906.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVW65_000992"
     CDS             complement(9091..11190)
                     /locus_tag="IVW65_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7899907.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVW65_000993"
     CDS             complement(11241..12062)
                     /locus_tag="IVW65_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7899908.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVW65_000994"
     CDS             complement(12059..12868)
                     /locus_tag="IVW65_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7899909.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVW65_000995"
     CDS             complement(12889..13383)
                     /locus_tag="IVW65_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7899910.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVW65_000996"
     CDS             complement(13395..13883)
                     /locus_tag="IVW65_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7899911.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IVW65_000997"
     CDS             complement(13960..16656)
                     /locus_tag="IVW65_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7899912.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IVW65_000998"
     CDS             complement(17084..18157)
                     /locus_tag="IVW65_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7899913.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IVW65_000999"
     CDS             complement(18154..18981)
                     /locus_tag="IVW65_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899914.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IVW65_001000"
     CDS             complement(18982..19788)
                     /locus_tag="IVW65_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899915.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IVW65_001001"
     CDS             complement(19800..20885)
                     /locus_tag="IVW65_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899916.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IVW65_001002"
     CDS             complement(20902..21444)
                     /locus_tag="IVW65_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7899917.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IVW65_001003"
     CDS             21702..22691
                     /locus_tag="IVW65_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7899918.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IVW65_001004"
     CDS             22688..23425
                     /locus_tag="IVW65_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7899919.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IVW65_001005"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IVW65_001005"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7899920.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IVW65_001006"
     CDS             23989..24621
                     /locus_tag="IVW65_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7899921.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IVW65_001007"
     CDS             24706..25353
                     /locus_tag="IVW65_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7899922.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IVW65_001008"
     CDS             25358..26341
                     /locus_tag="IVW65_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7899923.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="IVW65_001009"
     CDS             complement(26744..28258)
                     /locus_tag="IVW65_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7899924.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKVGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="IVW65_001010"
     CDS             28396..29202
                     /locus_tag="IVW65_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899925.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="IVW65_001011"
     CDS             complement(29377..29946)
                     /locus_tag="IVW65_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7899926.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="IVW65_001012"
     CDS             complement(30029..32089)
                     /locus_tag="IVW65_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899927.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFNPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
BASE COUNT         9661 a         6753 c         5490 g        10351 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ctatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccaactt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatta aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaat
//
LOCUS       DACCWK010000023        32182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCWK010000023 DACCWK010000000
VERSION     DACCWK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.023"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(122..502)
                     /gene="yajC"
                     /locus_tag="IVW65_001013"
     CDS             complement(122..502)
                     /gene="yajC"
                     /locus_tag="IVW65_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7899928.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(599..1744)
                     /gene="tgt"
                     /locus_tag="IVW65_001014"
     CDS             complement(599..1744)
                     /gene="tgt"
                     /locus_tag="IVW65_001014"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7899929.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1962..2660
                     /locus_tag="IVW65_001015"
     CDS             1962..2660
                     /locus_tag="IVW65_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7899930.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2828..5467)
                     /locus_tag="IVW65_001016"
     CDS             complement(2828..5467)
                     /locus_tag="IVW65_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7899931.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTEAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6086..7117)
                     /gene="queA"
                     /locus_tag="IVW65_001017"
     CDS             complement(6086..7117)
                     /gene="queA"
                     /locus_tag="IVW65_001017"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7899932.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7310..8485
                     /locus_tag="IVW65_001018"
     CDS             7310..8485
                     /locus_tag="IVW65_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7899933.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8489..9130
                     /locus_tag="IVW65_001019"
     CDS             8489..9130
                     /locus_tag="IVW65_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899934.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9127..10044
                     /locus_tag="IVW65_001020"
     CDS             9127..10044
                     /locus_tag="IVW65_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7899935.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10041..10712
                     /locus_tag="IVW65_001021"
     CDS             10041..10712
                     /locus_tag="IVW65_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899936.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11253..11741)
                     /locus_tag="IVW65_001022"
     CDS             complement(11253..11741)
                     /locus_tag="IVW65_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7899937.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11942..12208)
                     /locus_tag="IVW65_001023"
     CDS             complement(11942..12208)
                     /locus_tag="IVW65_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7899938.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12208..12471)
                     /locus_tag="IVW65_001024"
     CDS             complement(12208..12471)
                     /locus_tag="IVW65_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7899939.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12464..12736)
                     /locus_tag="IVW65_001025"
     CDS             complement(12464..12736)
                     /locus_tag="IVW65_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899940.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12947..13132
                     /locus_tag="IVW65_001026"
     CDS             12947..13132
                     /locus_tag="IVW65_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7899941.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13274..15097)
                     /locus_tag="IVW65_001027"
     CDS             complement(13274..15097)
                     /locus_tag="IVW65_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7899942.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15695..18400)
                     /locus_tag="IVW65_001028"
     CDS             complement(15695..18400)
                     /locus_tag="IVW65_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7899943.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18706..19653)
                     /locus_tag="IVW65_001029"
     CDS             complement(18706..19653)
                     /locus_tag="IVW65_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7899944.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19843..20112
                     /gene="rpsN"
                     /locus_tag="IVW65_001030"
     CDS             19843..20112
                     /gene="rpsN"
                     /locus_tag="IVW65_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7899945.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20288..20650)
                     /locus_tag="IVW65_001031"
     CDS             complement(20288..20650)
                     /locus_tag="IVW65_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7899946.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20661..21782)
                     /locus_tag="IVW65_001032"
     CDS             complement(20661..21782)
                     /locus_tag="IVW65_001032"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7899947.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21798..22148)
                     /locus_tag="IVW65_001033"
     CDS             complement(21798..22148)
                     /locus_tag="IVW65_001033"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7899948.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22278..23789)
                     /locus_tag="IVW65_001034"
     CDS             complement(22278..23789)
                     /locus_tag="IVW65_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7899949.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24111..25463)
                     /locus_tag="IVW65_001035"
     CDS             complement(24111..25463)
                     /locus_tag="IVW65_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7899950.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25616..26692)
                     /locus_tag="IVW65_001036"
     CDS             complement(25616..26692)
                     /locus_tag="IVW65_001036"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7899951.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26888..28633
                     /locus_tag="IVW65_001037"
     CDS             26888..28633
                     /locus_tag="IVW65_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7899952.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28913..29350)
                     /locus_tag="IVW65_001038"
     CDS             complement(28913..29350)
                     /locus_tag="IVW65_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7899953.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29334..30707)
                     /locus_tag="IVW65_001039"
     CDS             complement(29334..30707)
                     /locus_tag="IVW65_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899954.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31029..31517)
                     /locus_tag="IVW65_001040"
     CDS             complement(31029..31517)
                     /locus_tag="IVW65_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7899955.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31539..32141)
                     /locus_tag="IVW65_001041"
     CDS             complement(31539..32141)
                     /locus_tag="IVW65_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7899956.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9199 a         6884 c         5943 g        10156 t
ORIGIN      
        1 gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc tttctttttt
       61 gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata agctttgata
      121 cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca gtttttactt
      181 caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca tttgttacga
      241 cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag atcccttcgc
      301 agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca tgtaatccgc
      361 cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga cgttcttgct
      421 gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg ataaacatca
      481 caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta caaaaaacac
      541 tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag attttccttt
      601 agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct tctctaaatt
      661 caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc aacaagaagt
      721 ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg atcaaatggc
      781 ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca tcaattggac
      841 ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa gtcatacatg
      901 tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact ccacggataa
      961 caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg
     1021 ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt tccccgacag
     1081 ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc gcgctttgac
     1141 gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga cggtctggat
     1201 tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact gattttttca
     1261 cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg atatcagagc
     1321 ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc gaaccgttca
     1381 agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt aatgaaaaaa
     1441 cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat ttatgcagtc
     1501 cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat gtattactta
     1561 aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt actgtagcta
     1621 atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc
     1681 ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg gcaggttgcg
     1741 tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat cataatcgtt
     1801 tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat tctttttttt
     1861 atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata ttttttgata
     1921 catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct tctgcagaat
     1981 taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt ttactaaatc
     2041 taatccctac attgattggt attgctacta ttcttcttat tatgattcct attatcttgt
     2101 ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact tcaggaggcg
     2161 gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg ttcatgagcg
     2221 gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc gaaccattca
     2281 aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc
     2341 tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga atcattaaaa
     2401 gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa acaggacaaa
     2461 cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt tataaaggac
     2521 aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta gcaactgctg
     2581 gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca ttttatgaac
     2641 aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg gcaggagaac
     2701 accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac
     2761 aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc aacttagaaa
     2821 cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc agttcattca
     2881 ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta
     2941 ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta atagctaatg
     3001 ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg aagatcgatt
     3061 ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc aagaatgcaa
     3121 cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg acataactac
     3181 ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg gcaatttttt
     3241 gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga tccatgatgc
     3301 cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca actacattga
     3361 caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa
     3421 caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta cgaatattct
     3481 gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca
     3541 aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg gcacacccaa
     3601 cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc gctacgactg
     3661 cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat
     3721 agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga tgcagttctg
     3781 atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct atggcactgg
     3841 cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt ttcgctcgct
     3901 tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac
     3961 gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat
     4021 aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg atcgccgctt
     4081 gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct ttggtgattg
     4141 agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag
     4201 gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctctgtatga taattctctt
     4261 caacagccct tacgatggct acttctgttg gattaccttt gataacaagg tcgtcatctt
     4321 cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc atcgcgctgt
     4381 tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc cggcggacgc
     4441 gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta gcggttccta
     4501 gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc ttctgcacac
     4561 cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct gcaacagcca
     4621 aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca ccaatcacaa
     4681 atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc tgattcaatc
     4741 ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg atcttaccca
     4801 tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca gcaaccgtac
     4861 atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca gcaataaatg
     4921 ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct acacgtaatt
     4981 gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct aaaacaagaa
     5041 tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct cgtatgacag
     5101 ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg tttccttctt
     5161 ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt ccttcaaata
     5221 aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct acaagaagga
     5281 tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat tctttcggcg
     5341 cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct tgtgaagaca
     5401 acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc tgcttgtatt
     5461 cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat tttactataa
     5521 catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa gaaagcgtaa
     5581 aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata gatgtaagga
     5641 aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa gcaaacaaag
     5701 ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta taaatagtga
     5761 atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca
     5821 attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca aatgttgaag
     5881 atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt
     5941 atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac aagaaagccg
     6001 agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct tttttgttat
     6061 tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca aaactaaaga
     6121 aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct cttcctgcaa
     6181 aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt
     6241 ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt
     6301 tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa gttgttccga
     6361 cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc gcttcttccg
     6421 tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg ctgacaggtc
     6481 ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc tttgcttcga
     6541 tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca gcagaaccat
     6601 tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct ttgatatatg
     6661 gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct tcatactcaa
     6721 agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc aatctgccat
     6781 caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc aatgtctccc
     6841 acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca gtatctggtt
     6901 tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc gcatcaccag
     6961 gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca gttttccgat
     7021 ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg attaattctt
     7081 ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact ttctatcatg
     7141 tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt tttcacttct
     7201 attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt gttttaggca
     7261 tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt
     7321 tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc
     7381 atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa aaacgacgac
     7441 gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga ttgatggaaa
     7501 atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg ttatccaaag
     7561 tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt
     7621 gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc ttgtggaatt
     7681 gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc agcagcgtat
     7741 tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg agccattcgg
     7801 tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt tacaagagcg
     7861 tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta aactagcaag
     7921 ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt gatacaccag aaaatatttt
     7981 acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc tactacaagc
     8041 caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca cgattacacc
     8101 ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag atacgttgtt
     8161 agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt tgaataaaaa
     8221 acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt acgtaaaaga
     8281 atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc
     8341 ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat tagtggatat
     8401 cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag agtcagctac
     8461 tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc acggttcaga
     8521 aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga tcattggtat
     8581 catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg ccacgattgt
     8641 gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg cattgatgat
     8701 cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt attcattatt
     8761 gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt atcgggatgt
     8821 ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat tgcccattgc
     8881 tatgccaacg atcatggctg gtattcgttt agctgccgtt tacgtgattg catgggctac
     8941 acttgcctct tatatcggcg ctggtggttt aggcgatttg atttttagtg gactgaataa
     9001 ttaccaacca gatctcattt tggcaggtac gatccctgta acaattttag cactattggc
     9061 tgattggctt ctaggtctct tggaacaccg gctgacaccg atcgcactta gagaggagaa
     9121 tgaagaatga aacgtatact aaaaatgacc atggttcttt tctctcttct tatacttgcg
     9181 agttgttcct tccctggtct tgccagcaat acagatgaag acaccatttc gatcacaggt
     9241 ggaatcactt cagaagcaca gatcttagca agtctagttg ccggaatggt tgaacattat
     9301 acagataaaa ataccgcaat catcaacaat ctggctacta caactatcaa ccatcaagcg
     9361 atgatgaacg gagatgcttc aatttcagcg gctcgctaca caggaacaga tttaacgact
     9421 acattgaacc ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa
     9481 tttgagaaaa gatacggtca aacgtggttc ccgtcctata gttttgaaaa tacgtatgtc
     9541 tttcttgttc gaaaagacac cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa
     9601 aatgtagcgg atgaattagt agctggtgta gatacttctt ggatcaatcg taaaggagac
     9661 ggttatgacg gcttccaaga aacttatggc ttctccttca attcgatatt acctatgcag
     9721 attggacttg tttatgacgc agtcgaagca ggaaaaatgg atattgttct tggctactct
     9781 acggatggac ggatcgccag ttatgatcta gtcatgttgg aagatgatcg tcaattcttc
     9841 ccaccatatg atgctgctcc agttgtagac aacaagatat tagctgatac gcctcattta
     9901 aaagaagcct taaataagtt aggtcatacc atcagtacgg aaaaaatgca ggaattgaac
     9961 tacgaagcag ataataatct agtggaaccg tcagtagtag cacaacgttt cttacaagaa
    10021 aaccactatt ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt caacaatttt
    10081 tctattattt ccaggaaaat ggctcctata tctttgcaca atttatccgt cacttcctga
    10141 tttctatcta tggtgtcctc tttgcagcag ttgtcgggat tccggttggg atcatgattt
    10201 ctaggagacg taaattagca aactgggtga ttcgtttggc gaatatcatc cagacgatcc
    10261 cttctctagc catgatctcc attttaatca ttggattagg cctgggagtc aatgtcgtga
    10321 ttgttactgt atttttgtat tccctcttac cgatcatcaa gaatacgtat accgggatga
    10381 tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat ggggatgact gctagacaac
    10441 gactattcat ggtagagtta ccactctctg tttctgttat catggcaggc ataagaaacg
    10501 cgttagttgt tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg
    10561 gagatatcat tatccgagga accaatgcaa cagagggcac atcgattatt ttagcaggtg
    10621 cattgccaac tgcattaatg gcgatcatca cggactggct tttaggtatt ttggaaagac
    10681 ggctagatcc agccagccgt acatcacgct gatgttattg taaaaaacaa aagcttctgc
    10741 gaccatttgt tggtcgcaaa ggctttttta gttctgtcat ttttaaggca ataatccact
    10801 ctgattcttc cctccttatg ggcaaagagt tgcttttctt agctatttat ccattccgta
    10861 tactccaact tcatactgcc aacgggttca tatctgcatc caccgactaa gtttttttct
    10921 tatccgctaa ggcattttgc caatccattt tcccatcact cgtcatggaa ttcataccac
    10981 atgatctcta aatactcgtc tgcttgttct ttgaattggg catcaattct tacatccttt
    11041 ggcgtggctt tcatttaagc cttttccatc agtcctcgat tgttcgatgc cttcagctgc
    11101 atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc atcttttata caatcgggaa
    11161 cttgccatcc tcatccttgg ctaaaaacat atctagccat tccagcttct cgccatggga
    11221 ggtttgcggt tcctaaaacc ggtacacgat tcttatggac tgcatccgtc gcaactagac
    11281 ttggcgatac gatgatcctt caccagaaga gcacccccgt aaaccaattg atcgatatat
    11341 tgccaataag aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg
    11401 atactgctat tcatggccga aagcgccacg actttaactt cttccgtttg agtgacattc
    11461 atgttcggca atgtcgcttt ggatacccgc tctgctaaag gaacgacact ggtattgtat
    11521 ttcgtatctg tatcttttcc aaaagaccat gccaacgaat cctctgggaa ccaataagac
    11581 gactccggtt gattgtccat tgcttttttc aacatacttt tggctgtttc tcgataaacg
    11641 acatttccaa ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc cactgccaac
    11701 actaacaacg atgctccaat tttactgatt ttccttgtca cacttcttcc tccttgtttt
    11761 acataaaccc gtcaacattc cattcctaaa ctcaaacaat gacccttcaa aggtggagat
    11821 ataggaacaa attaaagaaa tcatgcaata aatccaagaa ggttggtttt agaacaaaaa
    11881 aagagtctcc tttttgggga gactctttgt taactatcta aaaaggttgg ctcttctcac
    11941 ttcactcata atgtgtccag gcagaataaa tttctacaac tttattttcc tcatcaactt
    12001 tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc
    12061 tcagcttttc aaaagactgc gtgggcatat atggatcttc cttcaatgtc tgaatcactt
    12121 cttcaaattg tttctttaaa ttcgtttgct tgatttttct taaatccact ttcgcagagt
    12181 ttttgattgc gactgtatag ttactcatca aagagcttcc caatcaatat catcgatatt
    12241 cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc
    12301 aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc ccaatcaata cagcattgtt
    12361 tccactacga tcactaataa tctcgatttc agtatgattt tcatttacct ctttcaataa
    12421 ctgataaaaa tttttacgag cagtgctagg attcatcgtt gtattagcca ttgtctctcc
    12481 tcctttttct gatataatta tagcgtactt gattaagtac gtcaaaaaaa tacaataaag
    12541 cacttaaatt cagcaaactt ttcctctttt ttacatagag aaaaagaccc ctttttaggg
    12601 aggatctttg tccacgctca cttcttactc ctttacgact agcggtagcc aaacgtaaag
    12661 taattggtaa caacgtatca tctttagcaa aggtcgaatc caaagcctgt gagtctatgc
    12721 aacaacacca atacacgaag gttcctcgtt gcagtgtgac ttgattgata tcttgaataa
    12781 cctgaggata cgcacgaaag atttgtaacg gttattcaaa actgattttg gccgtcaagt
    12841 cttctactgc ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt tcactatcga
    12901 tttgatactt gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta
    12961 tggtaacaaa aaatggtcac ttgacgaggc tgagattcct acttatgaag acacatggca
    13021 cttaagagtc gaggtaaagg aagaagctga aaatatagga ggctgtacat tatttggcaa
    13081 aggattcgga aattatgatc aagacatcaa aatcaaaatg ttttacagct aaataggaag
    13141 aataataaca tttcatacgc tgttcccatc tttaatggct tatagagggc acctttagct
    13201 gagaataaga caaaacagac cctctagctt caattaagag ctagagggtc tgtttttgat
    13261 tttaaaactt ggtttatttc ttcaaatggt agctataggt gctgacaacg atattttcac
    13321 gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt atggagaata ttctgccagc
    13381 gtctgtttgg tacaaattga ggaatcaaca cagtagttgt atagttttgt ttcgctgcat
    13441 ttttacttac gatatctaca taacggataa ttggattttc aatcgagcga taagaagagt
    13501 ggacgattga aaaacggaca tctgggaaat gctttttaaa ttctgcttcg atttcttttt
    13561 ctttttcgac atcttcgtct agcgaaacat gcatcgcaac gacataatca ccgatcgaac
    13621 gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt cccgactaat acaagaacgg
    13681 tattcccgtc ataatcatgt agttttgcat cattttctaa acgaagctgt tctgctactt
    13741 ttttataatg atcatggatc ttataaaaaa tgaacataac gattggcata atgatgaaga
    13801 aaggccaaat gtcccctaac cgatagacaa acaaaatcgc aatgattgca tatgagatga
    13861 acgcacccgt aatattggca atcgatttgc ttaaccatct ttttccttct tttttccatt
    13921 tgacgaccat ccctgtttga gataaagcaa atggaatgaa cacgccgata gagtataacg
    13981 gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta
    14041 gtgtgataat cccattggaa tagcctagtc gatccccacg atcttgatac atgtgaggca
    14101 tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag
    14161 cagcaactgc taaaatcagc gctgtagcaa attgaaggac gtaatacaaa ataccatgtc
    14221 caaagacagc tttaccaatt tgagacaaaa cagtgacttc tttttcagga acgatcccat
    14281 accaatagtt gataaatgtg atccccacga agaagaaacc taagatcaat gccatcatcg
    14341 tcaatgtagc agcggcattt tttgctcttg gcttcttaaa gaatggtaca gcattactaa
    14401 tcgcctccac cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga
    14461 tagaaacacc cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc acagcacctg
    14521 tcacgatatt atataaacca acaacgatca gtaacgagat cacaaggata aatgtgtaca
    14581 ccggaaataa taggaacgat gcagattccc tcagcccacg aagattcagc atcatcagta
    14641 gcaaaacgat tgtcacagaa atagcgactt gatgaccgta gagtgctggc accgctgatg
    14701 tgatcgcttc agcacctgct gagacagata ctgctactgt caacatgtag tcgatcaata
    14761 aagagccacc ggaaatcaag ccagcattct ttccaagatt ctcactgctg acgacatatg
    14821 ctcccccgcc atgaggataa gcatggatga tttgacgata tgatagcgtt aatgaaatca
    14881 ataaaataat aacaaaagcg gcaatgggta gcgaatacca gatggctgca gcagacaaag
    14941 cgactaaaac gacaacgatt tgttccgtac catatgcaat agatgataac gcatcagacg
    15001 acaataatgc caaagcagca aatcgggtta atttgtgctc atcattttca gcagatttta
    15061 aaggcttacc tactaacaac ctttttaagt aatccaattt ctaaactcct ttacacacaa
    15121 ttttacttca agttttatag tcgaaaattt atagtaaaca agatgttata gtacgctcac
    15181 tgaccttaaa agtcaataaa ctttaagaaa ataattggaa aaaaacagaa aaatatcaag
    15241 cgctttttca aacgctttaa tacagcaaaa atctaacatt tttttatggg ctttttcttt
    15301 gaattctata ataaaaggat agaacttgac ttagaaaatc aagttctatc ctttttgata
    15361 tcttaacttt tggaagagag atgtaaggct gctttctacc catcattctt actactcgaa
    15421 gcatagcgca gttgtcacaa cctcttgata aacggtgttt aaaagcttgc atctgcggta
    15481 ataagcccaa ccaaattaaa aacataagaa gctgttttta tttggtcgtt cttattactt
    15541 gatgcagagc agctttttta caacctcttg gtacacggtg ttcaatcagc tgaccctcgg
    15601 tattagctca aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
    15661 tcaggtcaaa acactgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
    15721 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
    15781 ttccaaagac aacaaacaaa ccgatcatga tccagattgc tttcgtagca tttggccaat
    15841 aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
    15901 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    15961 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    16021 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    16081 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    16141 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    16201 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    16261 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    16321 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    16381 ttgttgcgtc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    16441 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    16501 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    16561 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    16621 ccaatcctaa tttttgctga atgatcggta attcattgtt gtataggtct gccccttcat
    16681 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    16741 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    16801 cagttgcttt cgttgagctg agaagcgaat caaagtcgat ctggtccgct ttgctcagtg
    16861 cgccttgtgc agtcccaatc gtatcgatga cttggtctag tagagaagtg acagtggaac
    16921 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    16981 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    17041 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    17101 ttggctgtaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    17161 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    17221 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    17281 ttaacgaacc tgtcgttgtt gccacttgtc caattgactt caatgtaaca tctacactgt
    17341 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    17401 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcttgt acttgttgga
    17461 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    17521 caaaatcgcc gtcgatctct gctagttgct taccggcatt ttggatctct gggatttttt
    17581 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    17641 caacgacttt ttcacccatc tcatctactt tagggatgta atccacgaat tcatttgctt
    17701 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    17761 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatcttta actttattga
    17821 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    17881 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    17941 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    18001 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    18061 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    18121 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    18181 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    18241 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    18301 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    18361 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    18421 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    18481 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    18541 ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat aattttatgc
    18601 caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt gtctttgccc
    18661 ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt tttcgaatga
    18721 caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg
    18781 cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt tttggtaggc
    18841 tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa caaacgattc
    18901 ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta atgcaacata
    18961 cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc
    19021 atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt tatcataagc
    19081 agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg atgcactggt
    19141 taagattcgg attccctttt catggagaag ctggatcaga cttgacccga ccgcacctga
    19201 tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt tgattagatt
    19261 ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca ttttatctgg
    19321 aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc cgccgactgc
    19381 atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa tctctgtcac
    19441 aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta tctctttcat
    19501 ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta tttctacacg
    19561 aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc
    19621 ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt ttctttaagt
    19681 ttacgtttat gcctttattt tgacaaaaaa aatgtctttt cgttttggcg catgattcaa
    19741 aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga
    19801 ttacgattta cgctaataaa aaataattgg aggaatttgt tgatggctaa aaaatcaaag
    19861 attgccaaag caaaaaaaca gcaagaaacg attgaacgct atgctgcttt acgccaggaa
    19921 ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa
    19981 cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg gatacatgcg taaattcggg
    20041 atgtcccgta taaaattccg tgaattagca cataaaggtt tgattccagg agtaaaaaaa
    20101 gccagctggt aatccaagtc tggctgtaat gtaaaggagg agggcgcatg gaccactcct
    20161 tggatcatca aaaaagatac gttcgcttct tctagcagaa atgctggtac tagaagcaga
    20221 cgtatctttt tttacgcaca gctggtcctt ttttatatgt aaaggctgca tggttacttc
    20281 gctatattta tttagtaaca ggagtcagtg cttcgatacc cacactaatt ttcaatgctc
    20341 ggtcaacttc tgccataatg ggtcgatcca aatggcagac cttttctttc aatcttgatt
    20401 tatcgatcgt tctgatctgc tccaacagga taacggagtc tctttctatt cctgttccag
    20461 tagctttgat cccaatgtga gtcggaagct taggtttagc catttttgcc gttatggccg
    20521 caacaatgat cgtcgggcta aaatggttac ctagattatt ttgaataacc aaaactgggc
    20581 gtgttccgcc ttgctccgat cccacgactg gagaaagatc agcaaaataa atatctcctc
    20641 tttttaccat aggatcctcc ttattcttga tattctcttg gtatccgctg tcctaatccg
    20701 caagccactt catagtggat cgtacctaat tgatctgcta tatcctgcat agtgatttct
    20761 ttgtctgcat ttttgccgat aagcgtcact tttgttccga caggaaattc ttgaggcaat
    20821 cggatcatca gttgatccat acatacacgc ccaatcgttt cacagtagtt cccttctacc
    20881 aatagagaaa aaccctgcat ctttcgtggc cagccatctg cataaccgat cggtatcgtt
    20941 ccgatccact ctgcttcagg agtgatatat gtttctccat agccgattcc ttcacctgct
    21001 gggagttttt tgacttgaat caattcagaa accagctcaa gtgctggctg caatggataa
    21061 acttccggta gtgcgtgccc agagggatta agtccgtaca ttgctactcc ataacgaatc
    21121 atattgccga ttgtttcatc atgccataaa gccgttgcac tattgctgac atgtacatat
    21181 cgaggaagtt cgggaagttt tttcagcact tctataaagc gttctttttg cagattccaa
    21241 tatgtatcat ctgcctgatc tgctgtagaa aaatgggtaa ataaaccttc ccattctaat
    21301 acacgacttt ctttgatgaa tgcagctgcc tcttttactt cttcaggtgt acaaaatccg
    21361 atacgaccca tcccagtgtc tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt
    21421 ttctctaaaa cagcagctgc tgcttgcaac cattcccgtg tacctgcagt tactgataaa
    21481 tcatgagcaa cagctacagt cagtgcgtcg taaggaacca tattcaagat aaggatgggc
    21541 tgagtaaatc cagcttctct caactcgact ccttcatcta aatttgaaac acaaaagccc
    21601 gaagctccac ctgcaactgc tgcctcagct gtttcaatag ctccatgacc gtatccgttc
    21661 gcttttacta ctgcaaacaa ttcttttcct tgcggaagtc ttgcaacttc atttgctaca
    21721 ttttcagtta tagctttttt atgtatcact gctttcgttg ggcggtgcca agcgacaacc
    21781 atcgtttttc cttctttcta gtcttccaaa ataatttgtg caaaagctgt ttcatttgta
    21841 tgagtaatac tgacccatac attgccttca tgaggtgaac gcgtcacaac aggctgacca
    21901 ttttcatttt ttaaaatctc gacatcttga aaggtaactt ttccgatccc agttccccaa
    21961 gctttagaga aagcctcttt gcaagcgtaa cgcccgccta aaaattctac ttggcgatga
    22021 aaaggcaagg actggaatag tttcatttca tcagaggtca agattcgtgc aatgaatttt
    22081 ggtttttctt cgatcagccg agtgatccta ggaagttcga ctgcatcgat cccaattcct
    22141 tttatcatat gcttttctct tttcaataaa tttagaacac tctcattcta ccaaattata
    22201 accattttgc cacaaataag tccacaaata acaaaaatga aagtttctct taaaaaagag
    22261 aaactttcat tttatgttta atcgttattt gaacggatta caaatccgcg ttttttatca
    22321 ttgtgacgtt taccgccatc tttttggctg ttattatttt tgctataacc gccttttgat
    22381 ttgtttccac gataattacc gccgtcacga cgattacggt tgtttccgcc accacgtttg
    22441 ccatttttat tgtatccttt tttctgcatt ggcaatgggc gttctggtgt gatcttcact
    22501 ggcactgcat cagctggatc ttttgctgtc gtttttagca aaagtgcaac taaatcttgt
    22561 gcagaatatt cttctaataa tttatctgct gtttgcaggt atttatctag accattttct
    22621 gcaagttttg tttcgatttg ttcaactgca gcacctaatt gacctttgaa tgcttctttt
    22681 tcggttggtg gacgtaaagt cgtcatccgt tttttcgtca agttttcaat cacatgcaag
    22741 tagtccattt catttggtgt aacgaaagtc acagacatac cgccttttcc ggcacggcct
    22801 gtacgtccga tacggtgaac gtagctttct ggatcttgag ggatatcata gttgtacaca
    22861 tgagtgacac ctgaaatatc taatccacgt gcagcaacgt ctgtcgctac aaggatatct
    22921 aaatgaccgc tcttaaatga acgcaatacg ctcattcgtt tttgttgaga caaatcgcca
    22981 tggattcctt ctgcacggta accgcgtgct tctaatccgc gtgccaattc gtctacacgg
    23041 cgttttgtac gtccgaagac gattgttaat tcaggtgttt gaacatcaaa caagcgagtc
    23101 atgatatcga atttttcgta ttcttttgcg cggacatagt attgatcgat caagtcagct
    23161 gtcatttctt tcgctttgat cttcacatga tgtggatttt tcatgaattt aacgccaata
    23221 ttcttgatag ctggcggcat cgttgctgag aaaagtaatg tttgtctttg atcaggaact
    23281 tgtgaaatga ttttttcgat atcttccaag aagcccatat tcaacatttc atctgcttca
    23341 tccaaaacta atgtttgaac ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc
    23401 atacgtcctg gtgtacccac aacgatatgt ggacgatctt tcaagccacg gatctgacgg
    23461 ccaatatctg ctccgccgta aacggcttgt acacgtactt ttttgtcgcg ccctaaacga
    23521 tataattctt cttgtgtttg gatagctaat tcacgtgttg gtgcgataac taaaccttgt
    23581 aattcgtgac gatctggatc gattttttct aacattggta atccaaatgc agctgttttc
    23641 cctgtacctg tctgtgcttg tccaataacg tcttttccag ctaatgctag tggaatcgtt
    23701 tctgcttgga taggcgttgc ttcttcaaaa ccagcgcgtt cgactgattt caacaattct
    23761 ggtgataatt ctagttcttt aaatttcaaa tgaatcctcc tatatggttg ttaactgagg
    23821 atgtagcttt tttgctaccc cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac
    23881 agtggccacg cacgttttta tccattctta gcccctgtgg caccaccgaa aaatcacttt
    23941 gcgaagttta ggcattagaa aagccaaacg ggtcgcgttc agctttatca ttattatgac
    24001 atctggcttc aacaagttgt ttctatggca aaaccagtcc aatttctctt catctttcgg
    24061 caataggtta ttctaacata gtttttcgtt ttctgcaagt ttacagattt ttatttcatt
    24121 tgctgaagtt gttctaccac ttcaaataag cccatgctgt tgctgccttt tagcataata
    24181 ctatctgttg cttcaagatt ttgtttgata gcatcaatca atttattctt ttctttttca
    24241 aaatagaaca caggtagttg ggggtatttt tggttcaatt cttcttttag tgcatgcatt
    24301 tgttccccat aaaggaaaat catggcgaat ttctcaggat cgatatgttc actcatttgc
    24361 gcatgcatat ttaaagactc tttccctaat tccagcatat ctgctaacac agcgattctg
    24421 cgtccttttg ttggcagatt tttaaaagta tctaatacca gtcccatggc tgttggattt
    24481 gcattgtaga catcgctcaa aatagctgca ccatttcctg ccgttagcca ttcggttctg
    24541 ttttgagtga tagaaacatg ggcgagccct tggcgtatct caggtatgga cagtccaaag
    24601 aagcgcccga tcgtatacgc aatcaaagca ttcgttacat tataacttcc aggcagcggg
    24661 atcgtaaatg tttctccttc tactacaaaa tcggtttgtg cttttccttt tgctgtcact
    24721 tccgcatgaa catcgccatt tccaataccg aatgttgtga ctgtttgtac taatgttgcc
    24781 gttaaaggtg tcagcaacgg ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt
    24841 ccgtcagtga tttccatttt ggctttagcg attccttctc gcgacccaag attttctatg
    24901 tgtgcttcac cgatcattgt gattgctgca gcttttggtt gagccataag tgaaagttcc
    24961 gtgatttcat tggcgtgatc catgcccatt tccaaaacga tcttttctgt atcttggggc
    25021 atgtgcaaaa tcgtgtaagg aagaccaata ttattgttgt aatttccttg cgtcttatat
    25081 gttttgtatt tttcagacaa gacagacgcg gttagatctt tcgtggtggt tttaccattg
    25141 cttcctgtaa ttgcaacgac ttctgcgcct actttttcca agtaataggt tgccaattgc
    25201 tgcatggctt tcaaaggatc tgctacagga ataactggaa aattttttgg agcttgttca
    25261 acaggcaagc tccaaaatga agcagatgct ccgtttgtga tcgcttgctg gataaattca
    25321 tgtccatctc gcgtaccagc aagaggaacg aataaacttc cttcggttat cttccggctg
    25381 tcaaattcga cattggtaat ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca
    25441 atttcttgta ttgttagatt cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt
    25501 tggcctaaac ggcgggttcg catgactctc acttccaagt aaatttctct attcgtattt
    25561 agaagctaga atgaacgcgg ttacgcaatc actccagcct cttctttatt tctctttatt
    25621 catttttttc aaaaaaagat tgacgctgat ggtatcgatt cattcctaac tggatcagtt
    25681 cttcaatcaa atcaccgtat ttcaagccca tattttccca taagagtggg tacatactga
    25741 actccgtaaa tcctggcata gagtttaatt cattcaggaa taattcattt ttatttgtca
    25801 aaaagaaatc gcaccggctc aatccgcttc cacctaacat cgtgtaagct aacttcgcgt
    25861 actcttgcgc tttttgataa acttcttctg gcacttcggc tggaatctgc atttcgattt
    25921 tattattgat atattttgct tcataatcat agaatgctac gtcttttacg acttcaccag
    25981 gcaaagtcgt ccgaacgtct tcatttccta atacagcaac ttcgatttcg cgcgcttcaa
    26041 ttccttgttc aacgattgct cgagaatcat actgataggc tgttgctaaa gcattttgca
    26101 gctcttctcg gttttctgct tttgtaatgc cgacactaga acccatattc gccggtttga
    26161 caaacatcgg ataaagcaaa gaaccttcac attgatcaaa tactttttta ggattttctt
    26221 tccattgatt cttaagtact ggtacataag gaacttgcgg cacaccagca gcttgtaaaa
    26281 tatacttggt catgatttta tccatggcac atgcactggt caatacgcct gcgccgacat
    26341 aaggcatatt caatgtctct aagaagcctt ggatcgttcc atcttccccg tttggcccat
    26401 gtaaaactgg aaaaacgatg gctccttctt ctttgatttc gcccggattg atcacttttc
    26461 ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga aagatgcaag acatctttgc
    26521 tggcaggttt ttctgttagt aatggacctt tgacccattg tccttctttt gtaataaata
    26581 cgagttgaac ttggtaataa ttataataaa tggcatttaa aactgaaaat gcggaaagaa
    26641 tggacacttc atgctctgcg ctgcgtccgc catatagtaa agtaatcttc aaaagattgt
    26701 cctcctgtat tctaaatcgt tcagtgattt actctcatat tttagcacaa aacaaaaata
    26761 aacgaataac ttatttataa aaaaacgagc cagcttctaa aagaacgagc gtcatttatt
    26821 ttttgtcagc tagattattt gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg
    26881 gatgatgatg gcgcaaacaa atccacgcag ccgcttggca gaaatcattc gagtgttcat
    26941 caattacaat gtagtgccaa actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc
    27001 ctttgaagaa ctaggcccca ccttcatcaa gataggacag atgctttctg ttcgagaaga
    27061 tttgctttct tctgccttca ctcaaacatt caaaacatta caagatagtg tacctagcga
    27121 cacattttca acggtcaaaa agacgatcga aacagaactt tctctttcat tatcagatat
    27181 attcgatgat ttttcaaaat cgccttttgc ctctgcttct atggggcagg ctcatcgtgc
    27241 caagctgaag aacggtgacg cggtagtcgt taagatccaa caccctaaca tcgccgaaga
    27301 gatccgactt gatcttcaac tatttgaacg agcgatccct ttgatcaaat acattcctga
    27361 aactagcgta gttgatctaa aaggggtact ccaagaagta aaacgttcct tgatcaatga
    27421 aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg
    27481 gaaagagatc cgcagcccca agatttatga cgctttttgt tcgaaaaaag tgatcgtgat
    27541 ggaagaaatg tctggtaaaa acttgaatca tttaatgaac gccgaaaaca aaactgaaac
    27601 ttttatcact ggaattcaaa acaagcagct gaaacaagaa gttgcaaagc ttttagttga
    27661 gaactttatg aaacaggtgt ttgatgatgg ttttttccat gctgatcctc atccagggaa
    27721 tctgctattc catgtactaa caaaagaaga acagacacaa gctagccgaa aaactgaaac
    27781 agtccatgaa aaagagttcg gttcctttgc ttttcgagcc tccacttctg cagaagaccc
    27841 tgttgcgcca tataccatca actacatcga ttttggtatg atggggcatc tttctgcagg
    27901 acttaggcaa aaactcacac aagccgtttt agcgctgtat acgaaagatg cttatcgaat
    27961 cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg tttgatgaaa gtcggttcca
    28021 ccaagaactg acaagttttc ttgaacagta ttatgattct cctattgatg aaatcaactt
    28081 gcaagaagta ttcacacatg tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg
    28141 tgatatcacg cttttgctga aagcatttgg gacattagaa ggggtgatcc gcgtcctcga
    28201 tcctgaagtt tccctcatgg aagtcgcttc gccttttgct caacattatt ttctcactca
    28261 tctggatgta gaagatacat tgaaacaaag cggattagac ttattggaag ggatgaaggc
    28321 tgcgcctaag atccctcaac agctccacca tctattagaa atgtggactt cgggacaagg
    28381 aaaagtcaat ttggaattga aaaagcaaga caaattattg agcaggatcg aaagtatgat
    28441 caatcggctt gttttcggta tgattttagc tgcattgatc gtagggtcct ccctgcttgt
    28501 ccaagcagca cctgtagaaa acgcagaggt cgtttcctta ttagggatat ttacgtatgc
    28561 tattgccgca ttcgtcatta tttttttggc aatcgatgca ttgatccaaa tgtataagaa
    28621 gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt
    28681 tttacataaa ttgtttctca aatttttgtg aaaattcggc ttatttttcg aagaggctac
    28741 ttctgaaaca ccgagaataa aaaatgtgaa aaagctgttc tgcacccagt aataagaaca
    28801 atcaaaaagg gaacatggaa cacatttatc ctgtttccct gttccctttt tgatgtattc
    28861 gagcataaat aaaatacctt cgttacctac tatcttctgt acttggtaaa ccttagtcaa
    28921 ttgccttttt taacgcttga cgaaaagaca tatgatcttt ctccgtaaag ggttttggac
    28981 cctttgtcct tatgccagct tttcttaact ttccactatg ataacgttgt tctagaagcg
    29041 tgccgatatt cgcttcgtca aattcttttc cagagtgatg gaacactcgc acacccttcg
    29101 gcaatacaag tgaagctaaa ccataatctt gcgtgatcaa aaagtcattc ttcttaacca
    29161 taccgacgat tttataatcc gcactatcag ctccgcggtc tacgtagacg atttggacat
    29221 gattagcgta ttctttggtc gtataatggt ccacactggt cacgataacc acgtcaagac
    29281 cataggattc tcctaatttt atgacatctt cttttactgg agaaccatct ccatcaatga
    29341 atagcctcat tctttttcgc cttcttccat aaatgataaa cagttactaa tccgctcagt
    29401 aaaagaccaa cattgatcca ttttgccaag ataagcagtt gtacactttc tatcggcact
    29461 gattcatggt tcagcgtgat caataataga tggattggct ctcccatttc ttttgctaaa
    29521 acaaacaaaa gcgtcatcaa taaaaactgg tggatcacac tccctttatt ttgagtagta
    29581 tacttgacaa cgatccggcg atccattgga atagctgtat cagtttgata atattcctta
    29641 tttagccagc tgcctgttac tacgtgccag aaactaagca gaaagacacc tatcgcaaaa
    29701 ccatttggaa tcaagaggac caataaagaa gaactcagtc caagcaattg catagccaat
    29761 ggagaccatg ttaaacgttt caaaatccat ggtccgacta tttttgctgc aatcaagcct
    29821 gctgcataag gcaaatacat aaaaatcgaa agtttttcta atccgtacag tatgccaaca
    29881 taaaaactgc taaataaaaa aaggaaattc cctagcattc catttagaaa agtaaacaga
    29941 tttagccagc ttggcaactt ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag
    30001 atgaacagca gcaagaaacc ataaatagca agatcaaaaa gcctttcatc gaataacaat
    30061 cgggcatttc tcaaaagaaa caataagcca aagaatatca aaaataaaac aagctctcgc
    30121 gtagctacca gatttctttt gatgtctttg aaatccagtt cgtaacgcgg ataatgacta
    30181 accgtatggt aggccatgac gtaaaaaagt gtataaatag taaagcttgc tgcaatctga
    30241 gtcggtgcag gcaactcaat cgcttgatac aggagaaata gccagatagc tgcaaataag
    30301 tactttttcc catccatatt gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta
    30361 ttagcaggta caagccatgc tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa
    30421 ggaaaataaa atacgcctaa tattccaagc aatgaaccag caccaccaat cagcagagcg
    30481 atcatcagca acgtgtaact atctagacca aatcgtagac tgttgactaa aaatagtcca
    30541 gtcatccgaa aggcataaaa cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct
    30601 gttagtccca aaaataaaag ataaggaata aaaataaccg tgttacttaa gataaagggc
    30661 gttgtttgtg taaaattacg cccaaatcgt ttgagttttt cgaacatgac aatggctcct
    30721 taaacagtct tataactaat ctgctcttcg attttagcat taaaggggaa ataaaccaat
    30781 tttacagaat atattcttgt aaaaaaaaat acagaaagaa gcataagctt ctttctgcat
    30841 ttttacggta cttgattact caagcagaca agcccttctc cacaaaagtc ttgcccgaca
    30901 cccctgcatc cggcaggtga taaagataga aatcaattat cctgtgcctc caaaatcaat
    30961 taggagctct gtaaaccaga gtccttctcg acccgtcgca taaatttact ttaacacagt
    31021 tcctatgttt atgcgagagc ttttttcatt ttataatcga ccatttgaaa tccttgaccg
    31081 atatctgaaa taagttcttc tgtaatctca aatccttcat gcttataaac ctcgattgct
    31141 tgatgattcc cctgattcac tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt
    31201 tcttcatacc agcgaaaaac tgaagaagtc aatccttgac cacgtacttt atttagcaaa
    31261 tataatttac ttaaataaat ttttccattt atctcttcat aagcagtgta accgacagtc
    31321 ttttcttcgc tttttaataa aaagtaggac acaccttctg tcagttcttt ttgaatctgt
    31381 ttttttgact gataagtttt cagcatgtaa tccacttgtt tttgaccaat gatcggcaca
    31441 aaaacttccg gccatacttc ttgtatgact gccatcagct catccagttc attttctgtt
    31501 acttttattt tttccataca cccacgtcct tttttacatt ataaatgacc ttgcgcttga
    31561 tggaataaaa agagcatgat catgctatac acacccaccg accacggttt caagaaaatc
    31621 acaatctgca taaattccct aaaagataat ttcgtcaaac cagctactag acagatcaag
    31681 tcgtctggcg caaagggaag cagcaaggta acaactagta cttttttaat atctttcgtt
    31741 tttcttgtca gcaattcatc aaatttctga tacttttttg gcgataaaat aagctggaca
    31801 aaggctacgc catagtaacg gacaagcaaa aatccgattg cttccccaat cactaggcca
    31861 atacaactat ataaaagccc aaatccattg ccaaacatca gcatgcccgc tactgaggag
    31921 atccctcccg gcaaaatagg aatgatcact tggatcactt gcaacagaac aaatatcatc
    31981 actgcatact tgccaaattg ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc
    32041 ccatgatgat aagcataaag aatcaacaca aagaaaaaaa acagaccaat aatgggcatg
    32101 atctgaagac atttttgtaa aactgatcct tttttagaca tctatattcc ccacatcttt
    32161 tttcatttat taaaactgcc tc
//
LOCUS       DACCWK010000024        29077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCWK010000024 DACCWK010000000
VERSION     DACCWK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.024"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            68..808
                     /locus_tag="IVW65_001042"
     CDS             68..808
                     /locus_tag="IVW65_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7899957.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IVW65_001043"
     CDS             complement(1232..1402)
                     /locus_tag="IVW65_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7899958.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IVW65_001044"
     CDS             complement(1596..1775)
                     /locus_tag="IVW65_001044"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899959.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="IVW65_001045"
     CDS             2162..3274
                     /locus_tag="IVW65_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7899960.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IVW65_001046"
     CDS             complement(3352..3645)
                     /locus_tag="IVW65_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7899961.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IVW65_001047"
     CDS             3813..4127
                     /locus_tag="IVW65_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7899962.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IVW65_001048"
     CDS             4148..6127
                     /locus_tag="IVW65_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7899963.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IVW65_001049"
     CDS             6140..6466
                     /locus_tag="IVW65_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7899964.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IVW65_001050"
     CDS             6482..7000
                     /locus_tag="IVW65_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7899965.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IVW65_001051"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IVW65_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7899966.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IVW65_001052"
     CDS             complement(8436..9941)
                     /locus_tag="IVW65_001052"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7899967.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IVW65_001053"
     CDS             complement(10033..10623)
                     /locus_tag="IVW65_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7899968.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="IVW65_001054"
     CDS             10802..12448
                     /locus_tag="IVW65_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7899969.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="IVW65_001055"
     CDS             12497..13216
                     /locus_tag="IVW65_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7899970.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="IVW65_001056"
     CDS             13695..14642
                     /locus_tag="IVW65_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7899971.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="IVW65_001057"
     CDS             14654..15574
                     /locus_tag="IVW65_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7899972.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="IVW65_001058"
     CDS             15615..17072
                     /locus_tag="IVW65_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7899973.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="IVW65_001059"
     CDS             17417..18031
                     /locus_tag="IVW65_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7899974.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="IVW65_001060"
     CDS             18024..19748
                     /locus_tag="IVW65_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7899975.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="IVW65_001061"
     CDS             19745..21196
                     /locus_tag="IVW65_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7899976.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="IVW65_001062"
     CDS             complement(21669..23822)
                     /locus_tag="IVW65_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7899977.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="IVW65_001063"
     CDS             complement(23824..24870)
                     /locus_tag="IVW65_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7899978.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="IVW65_001064"
     CDS             25020..26309
                     /locus_tag="IVW65_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7899979.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="IVW65_001065"
     CDS             26322..29024
                     /locus_tag="IVW65_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7899980.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9453 a         5023 c         5898 g         8703 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtct tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt ataattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tagaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggcttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atacttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgagg cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aatttta
//
LOCUS       DACCWK010000025        28144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCWK010000025 DACCWK010000000
VERSION     DACCWK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.025"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(197..1096)
                     /locus_tag="IVW65_001066"
     CDS             complement(197..1096)
                     /locus_tag="IVW65_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7899981.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1106..1462)
                     /locus_tag="IVW65_001067"
     CDS             complement(1106..1462)
                     /locus_tag="IVW65_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7899982.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1817..2113
                     /locus_tag="IVW65_001068"
     CDS             1817..2113
                     /locus_tag="IVW65_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7899983.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2757..3710)
                     /locus_tag="IVW65_001069"
     CDS             complement(2757..3710)
                     /locus_tag="IVW65_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7899984.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3733..4488)
                     /locus_tag="IVW65_001070"
     CDS             complement(3733..4488)
                     /locus_tag="IVW65_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7899985.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4485..5444)
                     /locus_tag="IVW65_001071"
     CDS             complement(4485..5444)
                     /locus_tag="IVW65_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7899986.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5437..6387)
                     /locus_tag="IVW65_001072"
     CDS             complement(5437..6387)
                     /locus_tag="IVW65_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7899987.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6537..6989
                     /locus_tag="IVW65_001073"
     CDS             6537..6989
                     /locus_tag="IVW65_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7899988.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7078..9345)
                     /gene="gshAB"
                     /locus_tag="IVW65_001074"
     CDS             complement(7078..9345)
                     /gene="gshAB"
                     /locus_tag="IVW65_001074"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7899989.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9532..10530)
                     /gene="ftsY"
                     /locus_tag="IVW65_001075"
     CDS             complement(9532..10530)
                     /gene="ftsY"
                     /locus_tag="IVW65_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7899990.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10541..11350)
                     /locus_tag="IVW65_001076"
     CDS             complement(10541..11350)
                     /locus_tag="IVW65_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7899991.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11359..14940)
                     /gene="smc"
                     /locus_tag="IVW65_001077"
     CDS             complement(11359..14940)
                     /gene="smc"
                     /locus_tag="IVW65_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7899992.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14960..15646)
                     /locus_tag="IVW65_001078"
     CDS             complement(14960..15646)
                     /locus_tag="IVW65_001078"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7899993.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15994..17775)
                     /locus_tag="IVW65_001079"
     CDS             complement(15994..17775)
                     /locus_tag="IVW65_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7899994.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17801..18715)
                     /locus_tag="IVW65_001080"
     CDS             complement(17801..18715)
                     /locus_tag="IVW65_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899995.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18742..19704)
                     /locus_tag="IVW65_001081"
     CDS             complement(18742..19704)
                     /locus_tag="IVW65_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7899996.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19704..20648)
                     /locus_tag="IVW65_001082"
     CDS             complement(19704..20648)
                     /locus_tag="IVW65_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899997.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20649..21653)
                     /locus_tag="IVW65_001083"
     CDS             complement(20649..21653)
                     /locus_tag="IVW65_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7899998.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21921..22163)
                     /gene="acpP"
                     /locus_tag="IVW65_001084"
     CDS             complement(21921..22163)
                     /gene="acpP"
                     /locus_tag="IVW65_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7899999.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22285..23286)
                     /gene="plsX"
                     /locus_tag="IVW65_001085"
     CDS             complement(22285..23286)
                     /gene="plsX"
                     /locus_tag="IVW65_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7900000.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23335..25371)
                     /gene="recG"
                     /locus_tag="IVW65_001086"
     CDS             complement(23335..25371)
                     /gene="recG"
                     /locus_tag="IVW65_001086"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7900001.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25488..27170)
                     /locus_tag="IVW65_001087"
     CDS             complement(25488..27170)
                     /locus_tag="IVW65_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7900002.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27222..27584)
                     /locus_tag="IVW65_001088"
     CDS             complement(27222..27584)
                     /locus_tag="IVW65_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7900003.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27854..28042
                     /locus_tag="IVW65_001089"
     CDS             27854..28042
                     /locus_tag="IVW65_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7900004.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7780 a         6088 c         4956 g         9320 t
ORIGIN      
        1 tttttgtgat tatcgttcaa aaaaagaata cacgcttgca tctagtactc tgcgagttac
       61 aaatcatccg atcaaataaa cattcgctga ttattttccg caaaaaaagc agcatatcca
      121 cgataaatgg atctactgct cttacttatc cttatgtttt acaaggtatt ttcttttaca
      181 cattactaat gctgtctcaa tacggaacga ttgtcatcgc ttgttcaaat tcctcttgca
      241 aatgttgtgt ttgataggcc tgaaaagctg ggtgatgggc acgcaagagg acacgtaatt
      301 tcataggatc atagctgtct agcaactgtt ctttcaagac aaaatctcgg gtcagctgcc
      361 attcataata ataaccaaaa ggtctgcagc tgtaaatcgt gtgaccgatg gatttatctt
      421 caaagcgacg atgcgtatgg ccaaaaacta cttgtttgat atttgaataa cggtcaagta
      481 attctccgaa tgattctgat cctaaaaatg catttaattt gttccaacgc tcataaggag
      541 cagattgata catgataaat gcccgtttag gaacgaaatg agtggataaa atcacctcaa
      601 gtttttttgc ttccagctgg tctagcaaat tttttgcagc tgcaagcatt tctgcattga
      661 cctcgagatc tgttttccca cgctggatga accgatcata ccaatacaaa cgcttcattc
      721 gtacgattgc ctcgtaatct ttcaattcgg aaaactgata gtcgtaccag ccattcatcc
      781 ctaaaagaac ggtctgctca tttaaagcaa tataccgttc atttaaaaaa tgtgggtctg
      841 gaaaatggtc catcatctgt tcattgggca catctgccaa ctcgtgattg ccgaaatgaa
      901 atgtcgtaga taatccttta ttgacgaaaa attcgacaac agctaggcaa cggtctactt
      961 tattagccgt atctccggct agatgaagcc gagtgatctt gtgcttttgc aaaacttgcc
     1021 aaagctgcat cagctctgat tcgccgaatt tattgatgtc cacatgtagg tcgctgatga
     1081 tcgccaattt tcccatttta tccctctagt cttttcgaac aacttgcaag ttgccttttc
     1141 tcaagccgct tggtcctttt aattgtaaac tattataagt tggttctaaa gcaagtgtgg
     1201 atggatcatc catgtaaagt attgcactgt ctttgatttt gattggtcca actggtgtat
     1261 cagccactac agaataaaga cttagatctg tttgtgtatc ttttttgaca atgatcaatc
     1321 gaaatgccgt atccaattgg caactgactt ggctttctgc aaatggaccg tcaccgtttt
     1381 cgatatctaa taaaagttga tcgtctgctt ctaatttatt cttcaatact tcttgtgctt
     1441 cttttgtcac ttctaatttc atctgtatca cctcatgtca tagaataatc actttattca
     1501 tcagatgcaa ctaatttgtc tggttctgac tgttctcaat ttattggaaa gtaagtcatt
     1561 cgtttattat gatttctctt atcacaaaag agacaacttt cccttttctt cgttcagttt
     1621 cttgaacgga aaaagaccca aaaagcaacc tgttatttgt ttaaccttat tcctttctta
     1681 aataacaaaa aatactataa aaaataaaaa acctttaaca actattggaa aaagaattac
     1741 tttttgttca cggattatta acaactcttc ggtagagtaa acttatcaaa taaaagactt
     1801 ctttggagga atcactatga aggaatatat aaaaaacatc tattttatcg aagaaacaca
     1861 aaatatcgag ggttcttata ttgaagtaaa aacactcttt gtcaatgaag ataaaaccaa
     1921 agcattggat atttataaaa aattagcttc aaaaaaaacg aatagttttg gattgatatt
     1981 aagcgagtat aaaatcaaag cagaagagag ttatttctat caattactaa aacggtggag
     2041 taagcttccc gccgattttt atcgaaaaat gcagatcatc aactatcaac cgcttgctga
     2101 gacacatgcc taaatagagg ttgtgaaatc agtgctttga gctaataccg aaggtcagct
     2161 gattgaacat tgtttagaag agattgtgac aactgcgatt tgctttgagt aataagaacg
     2221 ctggacagaa aatagcttct catattttct agtctagtgg gcttattgcc gagaagcagc
     2281 agatggaaca tcgtttagaa gagattgtga caactgcgat ttgttttgag taataagaac
     2341 gctggacaga aaatagcttc tcatattttc tagtctagtg gacttattgc cgagaagcag
     2401 cagatggaac atcgtttaaa gaagagatcg gtacaaaata aaaatatcta aatttaagtt
     2461 ttatctaagt ttcgctttgt acctttgatt caaatcaaag gaggcgatct tgatgctgaa
     2521 aaaatggatc acactatttt ttgccactcc ccattctaga atcaacccgt atgatgtaga
     2581 taatgaattc acgttgaaaa aagatttctc aaataaataa aaaccatccg taaaatcatc
     2641 aatacgattt tacggatggt ttttgataac ttctttgagg ataggctctt gctatttaag
     2701 aatgtaagaa acattattgt tacatagtat gagagtttaa tcctctgtta tttatgctat
     2761 ttaaatgctt gattcacatc ttcgatcatc agtttcattg attctaaacc gcccccgctt
     2821 aaataccaga catctggttc aagggagatg atctgctgat ttttaccagc attcgtttgt
     2881 gctaccagtt cattggcaga aatatcatct ttgctgtcgt ctccaccaat agcttttgtt
     2941 ctgtctacta caaataagat atccggattt ttttccagca catattcata agaaacactt
     3001 tgaccgtgag tggatgcttc gatttgatca tctgcttgtt caaagccaaa taaatcatgg
     3061 atgaaaccga atctagaacc agatccgtag gctgataatt gcccttcatt cacaagtgtg
     3121 actagtgctt ttttgtcagt agcttctgct ttctttttcg tctgatcgat tgcactagat
     3181 aattcctcta attgagtttt agcttcttct tgcttaccaa agatctcccc taaagcagtg
     3241 acattttgct ggaaagattc ccatggattt ttcgcatcta aagaaaggaa aatggtcgga
     3301 gcaatttgac ttagatcttt ttgaaaatct ctttgtcggc cagaaatgat gattagatct
     3361 ggctgaagct gattaatttt ttctaagtct ggttctttta ttccacctgc tgattctact
     3421 tttttatagg atgaaagata ctcaggaagg ctgcttgtag ctgctccaac taccttatct
     3481 ccaacaccaa gagcatccat cgtatctaaa gaaccattgt cgaatacgac gactttttct
     3541 ggattatttg gcacctcgat ttggtctcca ttactatctg ttatcgtcaa tgtagtttca
     3601 gtttgtgtgc tgcttttttt tgtgtctgcc tgactggtgt tgttgctatt tccacatgct
     3661 gctaaaccaa atgatgctag aatcaagata cctactacta attttttcat ctcttctctc
     3721 ctaatgttag ttttagaaat aaagacagaa tctgcggcct tcaatctcac aaatccgtat
     3781 gtccatttcg tataattcat ccaatacatc tttttttatc atttcttggg tcgttccttg
     3841 cgtaaataac tgtccatctt tcattgcaac aatttcgtct gcataacttg cagcaaagtt
     3901 gatatcatga agaacaataa caattgtttt cccgatttct tgtactaaat tttttaaaat
     3961 ctgcatgatt tgaaccgcaa aattcatatc tagattgttc aaaggttcat ccagtaaaat
     4021 ataatccgtg tcttgcgcta gcaccattgc aatatacgca cgttggagtt gaccgcctga
     4081 aagagtgtca atcgattgat ccgctatttc ttctaaccca atgtgttcca gtgattcctg
     4141 aacgatttcc tcgtcttttg tcgtcaattt tcctttcgta taaggaaatc gtccaaattc
     4201 tactaattct ttcacggtga tttggacatt tatttcattc gattgtcgta ggatcgataa
     4261 ttttttggct aattcattgg atttccaagc ctttacttcg ttatggtcta aatagatttc
     4321 acctgtatct tttggtatca aacgactgat catcgacaac accgttgact ttccagcacc
     4381 atttggaccg ataaaagcag tcaacttctt ttcaggaatc gttaactgta catctttaac
     4441 cactgatttt ccatggtaca gtttattgac cttttgtaaa tcaatcatag gatactcttc
     4501 ctttcgcgaa tcaattttgt gacaaaataa attcctccga caaattcaac gacgacactc
     4561 actgttgtat tccagccgaa aatctgctct actgcgagct gtcctcctgt cagaaacagc
     4621 atagctacca gaaagcttcc gataaatagc tgaccatgac gataagtaga aaagacacga
     4681 taactgatat ttgcgacaat aaaccctaaa aaagtaactg gacccacaag agccgttgat
     4741 gtccctgtca aaagactgac acaacataaa atcaaaaatt caaaacgatc tgtgtctatc
     4801 ccgaggttta ctgcttgttc atttccgaga tggaaaacgt ctagcacatg tcgatatcga
     4861 aataacaggt accagcagcc gataatgatc aaagaagcag gcaaaagata ctgactctgg
     4921 atattgccaa agcttgcaaa taatctgcct tgaagcaaat catattcatt cggatccatg
     4981 atgacttgta aaaaggtact gacactacca aaaagcgtac ctaaaatcat ccctgacatc
     5041 agtaataaaa aaagattcgc tctcattttt ttcaaaagaa agaaaaataa caatagactc
     5101 agcccgacca tcagcccaac gttcaataag aaaaaaactg tacctgtctg ctgaagcatc
     5161 gtcactcctc cgataaagaa gaaaaggacc gtttggacaa ggacatataa cgactcaagt
     5221 cccaatactg aaggagtcaa aaactggtta tgcgtaattg tctgaaagct gattgtggca
     5281 ctgctgacag ctagcgagac aaggataaat gcaaaaattt tcttactcct caatgcacat
     5341 gcaaaatgcc agtttccata tgtatggatc gtcaagtaca ttcctgtaac aaataaaaca
     5401 agaacaagca taccaataaa aatagatggc ttttttttaa gcatatcgtc gtccccttat
     5461 cagaagataa aggaagatag ccccaccgat gattccaacg atcacactga ctggtatttc
     5521 ataaggaaaa atcaacacgc gagcgacaag atcgcaaaat actaagaaca aactacctgc
     5581 taaagcagtt acgccaattg ttttgtgcac ttgatctcca tatattctag agacaagatt
     5641 cggaatgatc actccgataa aaggaatgct tcctaccatg atcaacatga ctgcactagc
     5701 caatgatact aagccaatgc ctaaaaactg catcttctgg taatctattc ccagatttac
     5761 tgcaaacgat tcgcccagac caatgatcgt aaatcggtaa gcaaatagcc aaaccaagag
     5821 caacaatgga acaacagcat acaataactc ataatttcct cgtatcacag tcgcaaaatt
     5881 cccttgaagc catgaagtca tattttgtac caactggaat tgataagcaa aaaaagtagc
     5941 aactgaacca atgatattcc ctaacatgac tccaatcaac ggaagcatca attgattttt
     6001 ctggaggaac aatcgcgtaa gggataaaaa aagcaatgtt cctgcaaaag caaagcaaaa
     6061 tgctgtcaag gatcggacaa tcaaggatgc accaggtagg aaaatcatag ccactaaaat
     6121 acctaaacgc gcactatcca tcgtgcccgc agtcgttggt gaaacaaatt tattttgggt
     6181 caaatgttgc atgatcaacc cacaaatact actggtcgct cccacaagga tcaaacaaat
     6241 cgttcgtggc aaacgagtca ctagcaagat ttgtaagctt tgcccatcta actggagcag
     6301 ttctgacaat tgtacattcg atactccgat gaacaatgaa atactactta aaataactaa
     6361 taatgctact agtcgtccgt agcgcatcca atccaccttt ctaattgaga atgattttca
     6421 ttcttatttt aagtgaatcg ttgaacaaaa gcaaccgctt tttcatatgt tttaggtatt
     6481 attgggaata tgctataatc gtaagaaaga caaagagaag aaggagggta ttttttatgg
     6541 cacaagatga tgtaaaaagc catttggata agggacttta tggtacacct ttagtgaacc
     6601 ctgaagaaca gcataaatat ttagggacat tcagggaacg ctgctatctt agtatgactg
     6661 ttgctgaaat gatccaacca aaaaacaaag aaaacttttt aaaagaactc aaaaaacatc
     6721 cagatgcttc catactcttg aatggggctc tacccctttc tgtccaaaac caatatatcc
     6781 agctagcaac gaaaaacaat agccgtttca ctgtagtcaa cgattacgtc actgcaaatc
     6841 ctgaggaatt cggattgctc ttaacagcaa aagaagcagt aaatgaacct gtgattgata
     6901 tcgaaaaaaa atacccagaa gaaaactctt cgccagctga aaaagaaaaa ccaaaaaaga
     6961 aaaaaagctt ctgggatcgt ttattttaaa acgaaaaaac acataaattt ttacatgcaa
     7021 aaaaagcttt tgatcagatt gacatcaaat aggacaatct gtcaaaagct ttttctttta
     7081 aaatacttca ggatataaca atttcaatac attcatcgtc aatcttctgc cttttcctgc
     7141 aaaaggataa acatgcatat gcatggctgg attgaaattt gcttcgatga tcccatatgc
     7201 ttttttatca gttgtaggat cgatttcttt atctggtata atcaaatcga taccactaat
     7261 cttagctcct agtgcttcta ccgcagaaac ggcgattttt ttgtaagctt cagaaaattc
     7321 atcagtcata tcgatcgaat caccacctgt actgatattt gagttctccc ttagataaac
     7381 aatttgattg gcttggggaa ctgattcgat cgtcaaccct tgttctttca acatcaattg
     7441 ttctaattca cctaattgga tcaattctaa aggtgcacga tgattcgttc cacgcaaagg
     7501 gtcactattt ttttcttcaa caagttcttt cactgttcgg atactgtcac caataacatt
     7561 agcagggact cgcaacatga tggcttggac ttctccgtcg atcacaaaga aacgatattc
     7621 cgttcccggc ataaattctt ctactaaaac cgaactgtct tcacgaaaag caatggctaa
     7681 acctgcttga taatcttcca gactagctcc ttccttgaaa atcgtgatac ctaacccgta
     7741 attggttgat ttaggtttga tcacaaatgc ctgctcggca aatctcggat aagcttttac
     7801 cgcttcttcc atggatgaaa attctgcgcc tcctggtacc cggaacccag cttcttttaa
     7861 gacttttttc gttactgttt tgttttccat gataagtggg acgatatagc tgtccttact
     7921 tgtcatgttt gcatttttca catattctac atggtcttgg aattgcagac gcaaaaattg
     7981 atccgactca tctaatactt tgacttggac acctttttgg atggcatcga acataaatat
     8041 ctgagtagaa agttccattt cgcgaaaacc ggccaactga tagggacgtt catggctttc
     8101 tttataatat tgatgtccca atgccacagc aacttcttgc tgactcgata gctgtgcttc
     8161 ttgatagagt ttcgctgcta acgtttgttc aggatgatcc atttgagtca atgcttcctc
     8221 tactaaataa agagaatcca accaatccaa ttcaaccagc atttgcttca ttccttcaag
     8281 aatttctcta ccttcttgat gaaattctgt ttgatctgat ggttgttcta gtgctacttt
     8341 gttatttcga gtcgtacctt ctgctaccca ttggtcacag tcttcttttt cttctgtcca
     8401 taacatgtac atcaggaata attgtaagaa ccgaacttgc tctggattga tacccaatct
     8461 tgcataagga ttcaaatcga tattccgcaa ttcaatataa cgaacacctg ttgttgctaa
     8521 atctgcgact ttctttccgc ctcggaaacg aagcggtgta taaaattctt tctcctccga
     8581 aagtttccct tcttcgatca ttcgctcgat atctgctaaa tactgtttca tagaagcata
     8641 ggaaactttc acatttggat gattcgtata gcccagcgca ctgttgcgaa tactgcgaac
     8701 aggttcttgc ggatgtgatt catctaaaaa gtagttcttc tcactcattg gtgaagcacc
     8761 gaataaataa gtgatcatcc agcgatagcg cataaagttt ctcgctgttt tcaaataaag
     8821 ttctgttttg aattcggaaa aatcttgaaa ttcttcctga tggctgaaca atgttcttag
     8881 caactcatcg ccaaattcaa aattgaaatg aataccgctt accatctgtt ttcgcttccc
     8941 gtattctttt gctaaataac gtcgatacaa gacatcttcg aaatttttta attttgcaat
     9001 aatgatctct tcgtcttttt ccggtaaggc aggtggcata cttaatggcc agatcatctc
     9061 ttctttcggt atagaacgag cagtcacatc ataaacagca gcgagatact gaaataattc
     9121 gggaatagaa tcggtaacag gggtgatcat ctctgtttgt gtttcgctaa aatctgtttg
     9181 gatatagggg tgataggatc gatcgccaaa gattgctgga tgatcggttt ttgctagatt
     9241 tcctgcaaga tcaacccgct gtccttcccg ttctatacca aatcgagctt ggtcgataaa
     9301 agggcgcgca ttgacatgca ataataattg cttaaaattc atcattaagt gacctccaat
     9361 tcatatgaat ctaattataa tcaaaaagtc taagaaaaag aacgaaattg tttgagaacc
     9421 gcttacaaat aagtataaaa atgaaggcat caaaaacaca gctatgttaa aaacaatcat
     9481 tcaaacaagg agctgcaact tttgtcacag ctcctgcttt ttagacatgg gttattcttc
     9541 ttttagtaat cctttgaaca atccaacgac aaaatcattt ggatcaaacg gctccaagtc
     9601 atcgattcct tcaccaagac cgaccaattt cactggtaaa tgcaattcat tacggatagc
     9661 aagtacgata ccgccctttg ctgtaccatc taattttgtc aaaacaagac cggtcacatc
     9721 tgttgtttct ttgaattgtt ttgcttgaac catcgcgttt tgaccagtgg ttgcatctaa
     9781 tacaagaagt gtttcttggg gtgcttctgg taattcccgc tggattactc gttttatttt
     9841 ttctaattcg ttcatcaagt tcactttatt ttgcagtcgg cctgcagtat caactagtaa
     9901 aacatctgcc tgctctgtct ttgctctttc caaagcatcg aatacgacag ctgctggatc
     9961 gcctcctgca ttgccacgaa caacttcaac accagcgcgt tcgccccaga caactaactg
    10021 gtcgattgct ccagcgcgga aggtatctgc tgctgccatc aatacttttt taccttcgtt
    10081 tttgaactga tgggcaagtt taccaatact tgttgttttc cctacaccat taacaccgac
    10141 aaacaaaata actgttagtc cgtcttcttg gatatttagt tcatttactt cattgattcc
    10201 ttccgcttca tagagatcca caagtttctc gatgatcgca ttttggactt ctgccgattt
    10261 tttcgcattt ctgagtttca cttcttgacg taatgcttcg gtgattcgta tcgctgtttc
    10321 aaaacctaca tctgcaccga taagtgtttc ttctaattct tcaaaaaagt cttcatcgac
    10381 tgttcgaaaa ttggcaaaca actcattcat ccgttcacca aatgttttac gcgtcttttc
    10441 taaacccttg tcgtacttct cctgtacttc aacttcttct gttttctctt cgccagtaaa
    10501 agcacgcttg attttatcaa aaaatcccat ttgactcccc ctactctaat acaaattttt
    10561 ggatcacttt ggctacgcca ttttcttcat tgctggctgt cacgaaatct gctgcttctt
    10621 tgacgaaagg cacagcattc tccatcgcta cgcctagtcc agcatattgg atcatcggta
    10681 aatcattttc ctcatcaccg atcgccatga tttcttcttg tttcaaacca agatcttttg
    10741 ccaataaaga aataccataa gcttttgtaa caccttttgg cataaattcc aaaagcatac
    10801 ttcttgtttt gatgatctca tacatttcat ggaattctgc tggtatttct ttgattttag
    10861 gatctaattc ttcttggtca taagcgatta ctgccttatt aagcacaaat tcatctgtaa
    10921 tatcgcttat atccgctggt tggaattgca gcagcttatt caacacattg tacaaggatt
    10981 gcttccttgg ggcactaggc agctgtaaaa ccacattgtc agacaaaaca tctagtggca
    11041 gatccaattg ctgagccagt ttgtataagc gttggatatc tgatacttcc atcactttct
    11101 tttccatgat ttcaccagta tcattttttt gtaccaaacc gccgttgaaa gtgatgctgt
    11161 agtcaccttc atctaccagt cctaattctt gcagataatg cgccatcgca gctaaggggc
    11221 gtcctgtaca tagaacgatc ttgacccctt gctgttttgc ttgtgctaat gcttgtttat
    11281 tctcatctga aatcagcttt tgttcattta ataatgtacc gtccaaatcg attgctacta
    11341 atttgatcat tttcgttcct acactttcgt ttcgttgatt ttcgcctctt cattgatatc
    11401 ttccatccgt acagaaacga tttttgatac accggattcc tgcatcgtca ctccgtacag
    11461 tacatctgct gcgaccatcg ttcctttgcg gtgtgtgact acgataaatt gtgtgtcatc
    11521 ttggaaatcg ctcaagtaac gaccaaaacg tttcacgttg gcttcatcta acgctgcttc
    11581 tacctcatct aagatacaaa acggaacagg acagacacgg ataatggaaa agagcaacgc
    11641 gatggctgta agtgctcgtt ctcctccaga aagcaaactt agactttgca gttttttgcc
    11701 aggaggttgt acttcaatct caatacctgt tttcagcaaa tcagttggtt ccgtcagtac
    11761 taattcagct ttaccgccgc caaacatatt tggaaagaca attttgaatt cttgacggat
    11821 agcttcaaat acttctttga atcttgtccg cacttcatca tccatctcat ccatcgtttc
    11881 aaacaattgg tttttcgctg ctaacagatc atctcgctgt gttgctaaga atgtatgtct
    11941 ttcactgact tgttcgtatt gttcgattga atttaagttt actggtccca gtttttcaat
    12001 ctgttctttt aaggatgaaa cctttgttct actgtcttcg atatcggttg tttcttgata
    12061 gtcttgggaa gctttctcaa atgtcagctg atattctgac tgcagatagg tcaaatgatt
    12121 atctaaaatc atttcagctc ggtctttttg gacttctaat ttggattgtt ccgacagtcg
    12181 tgctttttgc tcacgattac gctctgccaa gactgcttca agcttatcga tctccttttg
    12241 ccttctatct cgctgctctt tggcttttgc caactcggct tttaatgttt ctcgctgagc
    12301 agataattcg ttaatttgtt tttccaaact ttcttctgtg acttcatgat cagaaaagtc
    12361 tgctgtcaat gtcgccaact gcttttctat cgcttcttga cgttcggttg cctctgcttt
    12421 ttggactcgg gcaccgcgga gctggatctg cagatgatta aactgctctt tcaagacagc
    12481 aagatctgct tgttcttgtg ctttttgact ttgtacttgt gctcttctgg cttccatctg
    12541 gtcgctttct tgacttaacg atttgatttc ttcgtcgatt ttttgtcgtt gcgattcaag
    12601 atctgtttgt ttgtctttca attcgttcgt ttgcttttcg taatcttcga taaactgctg
    12661 cagttcacgt gtctcaaaag ttgagatttg cttttctttt tcaaaacgtt caagttcatt
    12721 tatgatgttt tgtagctgat ttgtcgcttc ttgttcttca aaacgtaatt gttcgcctcg
    12781 tgtacgcaaa acttcttgtt cttcggctaa acgagctgtt tcttgttgca gctcctgaac
    12841 ttttttttct tgtgcttgga gttgtttatc tgcttcttca aattcactag tcaactgttt
    12901 caattcttga ttttgtacaa acaaactgcc ttggtttcct cgtttattcg ctccacctgt
    12961 catggatcct ccggcattca tcacatcacc ttctaaggaa accactcgat attgatagcg
    13021 gatcgtttgc gcgattgcat tggcactagt taagtctttg gctaaaagga tcgttcctag
    13081 caggttttga accacagttt ggatttgatc gggataactg acttgttcac tggcaatccc
    13141 tataaaccct tctacagctg ctgcttgtgt caatatatga gccggtaatt gtcttggttt
    13201 gattgtcgtc agtggaagga atgttgctct tcctcctctt tgctgtttca aatacgtgat
    13261 ggcttgccga gcatcttttt cattttcaac gatgacatgc tgggctgccc cacctaaagc
    13321 tgcttcgata gctaacgtga aatctgcagg aacatcgatc agttcagcca ctgctccgac
    13381 aatacccgaa agctgctgtt tatgctgcaa gaccaatcga accccttggt aaaaaccaaa
    13441 atagttttct tggatttctt gaaggctttt cttacgagca cgtagctgct ggacttgatt
    13501 cattaattga tacattttag gctgttcttt tgctaatgct tcttggaact ttttcccgtt
    13561 ttgttggatc atttctaact tctcttttgt ttcagttaaa gatgatttta gctgcgcctg
    13621 cttttgtgtc aattcttctt tttgtaagat caatcggtct actgatgctt ctacttccga
    13681 ttgcttggct aacgtctgct tgctttttgc tgtttcttgt atatattgac gttctagata
    13741 cttcagttcg tttcctacag ctgcttgttc ttgcatcaaa tcaacatatt gatctcttaa
    13801 ttctgccaat aattctttcg ttgatttttg gtatttttct aattcttgct gtgtttttat
    13861 gagatttgct tcagcttttt ggatctctgt ttctttttct gctgctgctt tcatcaagct
    13921 ttcttgaagc ttttcaaaat gtttaacctt tttttgtgct tctgctaatg aagtttgata
    13981 ttcttgcgag cttttttgtg tatgctttgt ccgctcctgc aaaacatctt tttgtccttc
    14041 tgtctgcttc agtttttccg aaagatctaa taatacttgc tgattttttt cgatcagacg
    14101 atcggcttgc gcattttctt tacgctgctt cgccaaaatg gattcttgtt cttggatcga
    14161 ctcagatagt ttgcctaatt ctagattgaa tttcgcaagc tgtgcttgtt tgttgtccca
    14221 atcttttttt gctgtcttga tttcagcaac catcaaagag acatcggttt gagtcagtgt
    14281 ttcttttagc cgtaaaaact cctttgctgc ctcactctgg gcggccaaag gagttaactg
    14341 ttcttccaat tcgtggatga tgtcttggac gcggctgagg ttgtcttctg tttcaaaaag
    14401 cttctgttct gctttctttt tccgctgctt gtatttcaag acaccagctg cttcttcaaa
    14461 gatcccacga cggtcttccg gtttactact gaaaatcgct tctacttttc cttgagaaat
    14521 gattgagaac gattcttttc ctaatccaga atctaagaac aattcttgga tatcttttaa
    14581 tctgcagctt tgtttattga tgaaaaactc gctttcccca gtccgtcgat aacggcgggt
    14641 tacactgatc tcattgtact ccaatggcag ataatgatca ctattgtcta aaatcactgt
    14701 gacttctgca atattcagct gcttacgggt atcggaaccg gcaaagatga tatccggcat
    14761 ttttccgcct cgcagacttt tagcagattg ttctcctaat acccagcgga tagcctctgt
    14821 aatattgctt tttccgctgc catttggtcc aaccactgct gtgacgctat tttcaaaatc
    14881 aatgactgtc ttgtctgcaa atgatttaaa accggctatt tcaattcgtt tcaaatacaa
    14941 agctttccct cacttacttt tacagtgctg ccaatgcatt ttccgccgca gcttgttctg
    15001 ctaatttttt cgatttccct tgtccagtgc caatcaattg atcatctaca taaacttcga
    15061 tccaaaatac tcgttcatga gctggacctt cttcattgat caatcggtat tcgattgata
    15121 catcacctga tttttgcaag acttcttgta gttttgtttt atgatccatc tcatgtgaaa
    15181 aagcacctgc tttgacttta ggaaaaatca ctttttcgat gaatgcatgg gtcgtgtcga
    15241 atccctggtc aagatataac gcgcctaaaa aagcttcgaa taggtcgcaa agcaatgctg
    15301 gtcgcgtacg tcctcctgaa ttctcttctc ccttgcccaa caaaacatat tgatcaaagt
    15361 gacattcttt ggcaaatttc gacaagctgt cttcgcgaac gatcgcagct cgtgtttttg
    15421 tcaattttcc ttctggcaaa tgaggatata aacggaataa atactgagaa accatcagtt
    15481 ctaaaacggc atctcctaaa aattcaagtc gctcattatc cgataattgc agattgcgat
    15541 gctcattcac ataggatgaa tgggtaaaag cttgttcaag caaatttaga tcgtgaaaaa
    15601 cgatgttata gttttctttt agttcttttg tcagctggtt gtccattttc cacattcctt
    15661 ttatggatat tttttcgata cttccttatt atactggttt tttgtcaaat ttgaaatggt
    15721 tcggtcaaaa ggtaagtaaa gctttaataa tcctagtgat tgacgagaag ttgtgaaaaa
    15781 ttcgtttagt ttcgagtatg aagaaaaaat tttgtgaaaa tggctttcca tatttttgca
    15841 taaatttgac ttaataccga gaaactggac tttgaacacc gtttattgga gaagtcatga
    15901 aatcagcgcc ttgacctgaa tattagagaa ataaacagaa aaatatcaaa aaaccgaaaa
    15961 aggacatcgc cctttttcgg tcatttttag tgattatgaa cgtgaatctg ctgtaacaga
    16021 aacatttgcc catgggtttg gtacatcata agaccaatcc cagtctttga cacggtcgtt
    16081 gactggcaat acttcattac ggaacaacgt tggaatcgca aatgcttctt caaatgcata
    16141 ttgttgccat ttttgatatg cttctttttg gtactcttca tcaaatgctt tgcttgaact
    16201 gatatcgttg atcaattttg tattttcttc tgattcataa cgagtgtagt tgaacgcagc
    16261 atttggtccc catagtccac ttggatttgg atcagttcct gtattccaag cagcttggta
    16321 aacatcaatt tcaggatcat cgttttctaa tttatcatag aaagaattga attcgatcag
    16381 acgacctgtt gtgtattgga catccaaacc aatttctttc cattgttgga tatagtaatc
    16441 tgccaatggt tgcgctgttt ccccaccgga catcgaagca aagttgattg ttagtttatt
    16501 tccatctttg tcttctctta aaccatcgtc atccacatct ttgtagccag catcatctaa
    16561 tagttttttc gctttgtcta gatcttgtgt gaatcctttc atctcatcat catggtatga
    16621 ctggaatact ggaggaatca atgaagtcgc attgcttctc aaacctgcat agaaacgttc
    16681 acctactgca ttattatcta atgcatagcc cattgcttga cgtaatgatt tatcagccat
    16741 ttttgaatct ggattgtatt ccacactctt tgtatctgca ttccatttac ctaacttgaa
    16801 tcctagataa gtataagaag tttgttcacg gcctaagttt gtatagccag caatttcttt
    16861 ataggtatcg taagtatctg ttggcatttg gaaaatcata tcatataatt tagagttcaa
    16921 tgcttcagtt gcacttgcca tcggtacaga tttcatcgtg atctttttca atttaggcgt
    16981 accattccag tagtgctcgt ttggcaagta ttctactgat tctcctgcaa cgatattact
    17041 catgtaataa gggccaaaag tcactggatt tttacgtact tgatcgcttg attccatatc
    17101 tttgattgct acaccttcta acgtatgttt tggtgctgca tatgaccaga taccaccacc
    17161 cgattgaagc atgcttgggt ttacttcttt atattcgatt tcaactgttt tgtcgtctat
    17221 ttttttgatt ccggagattg tgtcggcact tccatcatga tattcttcca tacctacgat
    17281 gttacggaaa gtgtcgtcat aacggatacc agtgtaatct ttattcccaa tgatttcgta
    17341 agggaaaatc acatcgtccg ccgttacatc ttcaccatct gaccatttga cgttgtcttt
    17401 tactttgatt gtcgcttttt tcgtatcttc atctagatca agcgttgcag ctccgtcgtc
    17461 tgtgattctg aaatcgtcat cgtttgcaaa tagcggttca tgagaaggag ccataaaagc
    17521 tgcatcatat gaatcttggt aaaactccca ctggaacaat cctttaaact gactgtccgt
    17581 tgcgaccgca gcttctaatt cgccgtcttc gattgcatca tcattatttt ttgttgcaat
    17641 tggaaaagtt aattcctctt tgttattgcc gttgcctcca gagttgtttc cagaggtatc
    17701 tgtacctccg cccccgcatg ctgcaagaac gacagcagac atcagcgctg caaatccaaa
    17761 aatcgttttc ttcattccta tttccccctt cgcttctata ctagcctaac cgttggcgcg
    17821 catctgcaga acgcttgaat gcttgtccaa cataattgat actcaacatc aaaactaaaa
    17881 taaggattga tgcgggtaac cagatccatt gccgattcac taagacttct ccgttacttg
    17941 cataaccgat caatgtacct aaactaggta catttgccgg caagccgaaa cctaagaaag
    18001 tcaaagttgt ctcgataccg atgtttgccg caaagttcat cgtcaaatta gtaatgatta
    18061 atgaacttag attcggcatg atttcgcgga acatgatttt aaaatcattg gttcccattg
    18121 tttttgaggc actgacataa tctcttcttg cttcagataa tgtcttgctt cggaataatc
    18181 gggctttagc tacccaataa aatgcactca tgatgaatat aaaggaccag acattgtatc
    18241 tggaaataat cgatacgaag acgataatga tcagcatgat cggtagaatc atgataaaat
    18301 caacgatccg catcaagatg ttgtcaatga tccctccgta gtagccagag atgatcccta
    18361 atccaacccc gatgatcgaa gtaaggatcg tgatcgcaaa tccgatcgta acagagtttc
    18421 tagcacctat gatcaattgt cctaatacat cacgtccgcc ttcatctgct cccagaatga
    18481 acttttgtcc ttgtgtcgaa actgttcctg gtgcagcgta tttgtcgaag atactaacat
    18541 acatcacttt gtcttgatcg gtcagcatcg caccgataaa gatcactaat aataaaacca
    18601 ctagcaaacc taatgaaaaa atagcaattt tatctttttt aaattctcga gcaatcattc
    18661 gtatccccat aggaggaata ctttcttgaa ctgctgtatt gtttttttca ctcatcgatc
    18721 cattctcctt tctgattggt tttattgaat ccgaatacgc gggtcaacga tactcatgat
    18781 gatatctgaa agtaatgtcc ccactagcgt cgcgattcct aaaatcaata ctaaggatgt
    18841 gatcacggta tagtcccgtt ggccgatgga atcaataaat agttttccga ctcccggata
    18901 accaaaaatc ttttcgataa ctacagaacc agcaatcaac gaagtgattt cataccccat
    18961 ttgagaagcc atcggcaacg ctgcatttcg gaaaatatgt cgactgaata ctttattcgt
    19021 cggtacccct ttggaacggg ctgttctgac aaaatcgagc gatttcgaat cgattacttc
    19081 acttcgcaga tattggattg tgacggcagt cgccaaaaat gcttgggtaa ttgatggtaa
    19141 cagaatatat ttcaaacggt ctatccattg agtgataaat ccaccttgca aacctagtgt
    19201 ttgtgatcca cttgttggga accagcctaa acgataacca aaaacaaata gcatgatcaa
    19261 gccgaagata aataaaggaa cagcaaaact aaagaagttg tagattacga caatcttatc
    19321 caagattgaa ttttgatacc ggccagcaag catccccaga ggcacagcaa tcaaataagt
    19381 aaggacaact gtcaagatcg atagatataa agtgttgatg atccgttcgc cgatcaagtc
    19441 agcaacagag cgtttgtata agaaactttg accaaaatcg ccttgcaaag catttccgat
    19501 ccaacggaag tactggacat accatgggtc attcaagcca gctgcttgtc gcatctgttc
    19561 gatcactgcc ggatcctgat ttggattgat caaaccggta aatggatccc ccggcatcgc
    19621 ttgggctaat aagaagatca gaacacttaa aataataact tgcgggatca tcaataaaac
    19681 acgtcgcaga atcgttttcc acattagctt gcacctccat ctttcaacgc cactttgtgt
    19741 gtagttgtga tcgtacgcaa atcgtagact cgaccagtcg catcatagta ttctttttgg
    19801 ttttggatat actcttgttc tactcttcgt cgattttctt tgtggctttc cctattttct
    19861 acatcgattt gcggaatagc tgaaagcaga cgtttcgtat agatatgtcg cgggtcatta
    19921 taaatgtctt ctcttgtacc gatttctaca aatcgacctt tgtacataat tgcgatattg
    19981 tcgcacatgt gttttactac acctaaatca tgtgaaatga ataaatagct gaggccgtat
    20041 tcttgctgga ttcgtttcat gaaattcaat acttgcgctt gaacagatag atccagagca
    20101 gatacaggtt catcggctac gatcagtttt ggattggtag caacagcacg ggctactcct
    20161 agacgttgac gttgtccgcc tgaaaattca tgaggatatt tatatagcgc atcttccggc
    20221 atcccaacga tatctagcag tccttttacc tttttctttt cttcttgatc gcttaaccgt
    20281 tcaaaatttc ggatgggttc agcaatgata tccaacactc gctttttagg attcaaactg
    20341 gacattgaat cttggaagat catctgcaca tccttgttat aacccatttt tttgcggttg
    20401 cttcgcttcg ttacatcttt tccttcatac atgatttgtc cggctgttac cttttctaat
    20461 cccacaacgg cttttcccgt agtagatttt ccagagccag actctcccac taaaccatat
    20521 gtctttcctt tttcgataat aaaatccaca ccatctacag catatacatg atccgttact
    20581 cgattgaaga atccactgcg aatagggtaa tgaactttca ggtctttgat tgtgattaat
    20641 tctgtcatct acacctctcc tttttcgtct ctaaagtgga aatgcttata gcaggagcaa
    20701 cgtaccaagt ggttcggtgc gacttcatga aggataggct cttcttcatg atcgcttgct
    20761 ggtatccatg gaatacgtgg tgcaaaccga caacctttac gaggcatgtt tttcagagaa
    20821 ggaacgacac cttcgatcac atggagttct gcatcatgat ttccttcttg tggtatagag
    20881 ttcagtaaag agcgtgtata aggatgctta ggatgatcaa acagttcttt tacacctgct
    20941 acttcaacga actgtccgcc atacatcact gccactcgat cagccatttc tgctacgact
    21001 cctaagtcat gagtgatcaa gatgatccct gtttttgttt cttcctgaag gtctttcagt
    21061 aaatcaagaa tctgtgcttg gatggttaca tctagggctg tcgtcggttc atctgcaatg
    21121 acgattggag gtttgcaggc gattgcgatt gcgatgatga cacgttgtct cataccgcct
    21181 gatagttcat ggggatattg gcgtccaact ctcgcaggat taggaatccc tacttgttct
    21241 agcaattcta gtgcacgagc ttgacgctgt tctgcattca tctttgtgtg ataggaaagt
    21301 ccttctttga tctggtcttc gattctcatg agaggattta atgcagacaa cggatcttgg
    21361 aaaatcattc cgatatcatt tccgcggatt ttattataga gcgtttcgtt caggtcgatt
    21421 aaattcagat cgttatataa aatctcacct gtaatttttg tgttattagg atcaagtaat
    21481 cccataatcg tagtagccaa ggtacttttc ccgcacccag attccccaac gattgctaaa
    21541 atctcgtttt tgtctaaagt aaatgaaacg tcatctactg catcgtaata gtcatctttg
    21601 agtcggaatc ctgtatgtag gttctgaact tctaataatt ggttatcgga caatcgaata
    21661 cccctctctg cttcctaaat ggaagttttt cttaataaaa aaggacatta cacagaataa
    21721 atcgttttat aacaaaggtt attcttatct ttgtgtaatg aattattaat aattatatag
    21781 aaaaccgctt tactttgcaa cataatttct gtttatcgcc agaattatca gaaaaatgac
    21841 ttttacgtgt caaaaagatt aaaaaaagaa ggataaaaag ctgcatgcag ctttttatcc
    21901 ttctttgctt ttatttgaaa ttatttaaga tggctgctga tataatctac tgcgccaccg
    21961 actgtttcaa tttgttcagc gtcttcatct gaaatttccg taccgaattc atcttcaagc
    22021 tctaaaacga attccataat actaattgaa tcagctttta aatcatcttt gatgttcaat
    22081 tcatccgtca ctttatcggt atctatctca aaatgatttg aaataatttt cgctactttg
    22141 ttaaatactt cttcgcgagt caactttttc acctccaaga atcatacact tgtcaattac
    22201 ctacaccatg gctaatttta ctggcatttt tcaggaatgt ccaccttttt ttcaatggaa
    22261 cggatcgctt cagatatcca aatactattc ttcgcttttt tcaaattgtt cgactaattg
    22321 tccaactaca tctgtctcaa gcattgtgtg gatttggcgg atcgtatagc gaacagcatc
    22381 ggggcctgtt gctccatgag ttttaatgac tggtgctttc aagccaaaca aaacggcacc
    22441 gccatgtttg gaataatcca tttctgcttt caaagaacgt aagccgtctt ttaaaagcat
    22501 agcgcccatt tttcctttga tcccttcatt tagaatagca gattttaata gattcatcat
    22561 attcattgct gttccttcaa tagatttcaa aacagcgttg cctgtaaatc catctgtgac
    22621 cactacatca gccacaccat tgagtagctc acgagcttct acgtttccaa taaaattcaa
    22681 gcttgtttca ttctgaagga gttcataggc ttttttcgtc acttcactac cttttgttgc
    22741 ttctgttccg ttgtttaaga gtgcgacacg aggacgtccg atacctcgga ctttacgtgc
    22801 ataaaaagaa cctaaaatac cgtattgcaa caaatgttct ggtttgtttt ctgcgttagc
    22861 gccaagatcc agcatatcga aaccggcacc ttctttccct actactggca aggtagacat
    22921 caagccaggg cgttcgatat ttttgatacg tcctacgatg aacaaccctg ctgctagtag
    22981 cgcgcctgta ttccctgctg aaaatacagc atctgcttca ccgtttttca ctgcttgtgc
    23041 agcaaggacc attgatgcag tcttttttcg tcgaattgct tttactggct catcatcact
    23101 attgattttt tcgtctgtat ggataatcgt gatgtttttt tcatttgtta agtatttctt
    23161 gatttctgct tcttttccgt aaagtaagaa ttctacttct ggaaaatctt gctgcgctaa
    23221 agccacgcct tctacaattg cttgaggtgc gtgatctccg cccattgcat ctacggcaat
    23281 tttcattgtt tccactcctt tttccgtcat cagtataaag tatagcacag tctgctaatc
    23341 gaaataacga ttatcctgat tttctatttg gaggctttgc gctaaaggct gatactcagg
    23401 aagcatctgc caatccttta tttgccaaag ggctgcagcc tcttctctgg ctacctccaa
    23461 aacatttcca tccgtcacca agtcagcaat cgcaaattga ggcagaccag attgacggaa
    23521 gccaaatact tcaccaggac cacgtaattc cagatctttt tcgcttagta caaaaccatt
    23581 ggtggtctct gtcatgattt tcatccgttc cacacctagt tcatttttag gattagcaac
    23641 taagatacag taggaagcat cacttccgcg tccaacacgt ccacgaagtt ggtgcagctg
    23701 tgccaatcca aatcgatccg catccatgat cagcatgacg gtcgcgttcg gcacgtttac
    23761 ccctacttca attacagtag tcgaaactaa tatctgcatc tggttgtctt tgaaggcttc
    23821 catgattact tctttttcct gattcttcat tttaccatgt aacaagccaa cttcatattt
    23881 tggtgcaaat agttcacgaa gtttttcata gatttccact gcatttttta catctaatgc
    23941 ttctgattct tcaatcagcg gacagatgac atacatttga tgtcctcttg ccagttcttt
    24001 tttcgtccaa tccagtacac tgtccaattg aggtgttctt acccagcgtg tttcgatcgg
    24061 gatacgtcca gctggaagtt catcaatgat agatacatcc atctcaccat atgctgtaat
    24121 agctaatgtt ctaggaatcg gagtcgctgt cataaataag acatctggtc gccagccttt
    24181 ttcacgaagt actttccgct ggttcacgcc aaaacggtgc tgctcatccg taataactag
    24241 acctaaattc tgaaattcga ctccatcttg gatcaatgca tgtgtgccga tcacgacgtt
    24301 aatttcccct tgctctagtt tttcaaggag ctcacgtcgt tcttttgctt tcgttgaccc
    24361 cgtaatcaat gcgatggtta cttcattggg atcaaacagc tgttgcaagc tttccatatg
    24421 ttgttgtgcc aagatttctg taggcaccat caacgcacct tggaaacctg ctgtaacggt
    24481 agcgtataac gcaatcgcgg caaccactgt ttttccgcta cctacatctc cctgcaaaag
    24541 tcgctgcata tgctgcggac tgcgaagatc ccgacagatt tcattcgtta cacgtttttg
    24601 tgcatttgtc agttcaaaag gtagcttttg agtaaattgt ttcaattttt ggacatcata
    24661 aaggatctcc aaaccattcg tttctgcttt ttctgctttt ttcaatcctt gaagacgcat
    24721 ttgaaataaa aagaactctt caaaaacgac acgtcgtttg gcttgatggt tttcttcgtg
    24781 gtcttttgga aaatgcatac ttcttacagc agaagctcgg tcaagcagtc gatatttctc
    24841 cacaaggttg tttggcagat tttcttcaat caatgtgccg tattcttgaa accctttttt
    24901 gattagatca actaatgtcg tttgacgaat tgatttgttg acgtgatata tcggcgaaaa
    24961 gtcatccatt gtttgagaac ctaaaatctt catcccgttc aaggctttac gctttgcatc
    25021 ccatttaccg tatacggcga tttcctccga taaaatgact ttttctttta gataaggttg
    25081 attaaaaaaa gaaacgttga acacatcatg ttcttgcatc atacgaaatt gcaatcggct
    25141 ttttttataa ccaaatcgat tcatcacagg aggcgagact acgattcctt tcaaagtaac
    25201 cttttcttga tcctggatct cgtttagatt tctttcctga atatcttcgt agcgaaaagg
    25261 ataataagtc agtaagtctt caatcgtttg gatgcccaag ctggcaagac tagctgctct
    25321 tttttctccg actcctgcca gcatagtgat tggatcatct aaagaacgca taaatttttc
    25381 ctccctcctt taacaaaaaa aggacttggt cagcgatcat acctgatcac taaccgaagg
    25441 ccttttatga atgaatactg tgttagaaag atattctgac acagaaatta ttcagcagaa
    25501 aatagataag gatagacagg ctgatctcct tggtggatct cgatttccaa atcaggatgt
    25561 ttttctgtta gtttctcagc aaattcattt gcttgttcaa ctgttccttc atcacctgcc
    25621 aagatcgtta cgatctctgt gtcctcattg atcatttgtt ctagtgtact taacgctgtt
    25681 gctgtgatat ctgggttaga gatcaggatt tttccatcga tcatgcccaa aaagtctcct
    25741 tctttgatct ctacaccgtc aattgaggta tctctgatcg cgtgagtgac ttgaccgctc
    25801 acaacgctag acaacatttc tgtcatcgtt tctttgtttt cttctaaaga agcttgatca
    25861 ttgaatgaaa gcatagctgt caatccttgg gaaatcgttc ttgaaggaac aactgctact
    25921 gggatatctg ctacttctgc tgcttgatca gctgccatga agatgttctt attgtttggc
    25981 aagacaatca ctttatctgc gtttacttct tgaatcgctt taacaatgtc ttccgtactt
    26041 ggattcattg tttgtccacc actgataaca taattagcgc ctaaactttc aaacagctct
    26101 tggacgcctt taccagcagc gatcgctacg ataccatacg ggatacgtgg tatttctttt
    26161 ttcgcaaact cgttcacttc ttcatcgtgt tccaaaatcg tctcatgctg caagcgcatg
    26221 ttgtcgactt tgattttgac taatgaaccg aatttttgtc cataattcaa gacttctcct
    26281 gggttttctg tatgcacgtg gactttgatg atctcgtcat cattgaccac aagtaaagaa
    26341 tctcctatac catctaaata gttacggaat tcttcgtaat cgaatgtact atcgactgta
    26401 ggtccttctc cgatttttac catgatttct gtacagtaac caaaatggat atcttctgtc
    26461 gccatttgac cttggacact gcgatgatgt tctgcattga ccatatcatc catttctgct
    26521 ggagaaggtt catatgtttc atccgcttgg aattctccat tcaatgcact taaaaagccc
    26581 tcataaacaa acagcagtcc ttgccctcca ctgtctacga caccgacttc ttttaggact
    26641 ggaagcaagt ctggagtttt agctaaagca cgctttgctc cttttacaac agctgtcatt
    26701 acttcaacga catcatcagt actttttgct ttcttttctc cagctttcgc tgcttctcta
    26761 gctactgtca aaatcgttcc ttctactggt ttcattactg ctttatacgc agtttcaact
    26821 ccatgagtga aggctgcagc aagatcagaa gcatttaatg agacaacatc tggaatttgc
    26881 ttagaaaatc cacgaaacaa ttgagacaaa ataacgccgg aatttccgcg cgcacccatc
    26941 agcaatcctt ttgaaagcat tgctgctaat tctcctactt tttctgaggt tgaatctact
    27001 actgccttag ctccactagt catagataga ttcatatttg ttcccgtatc gccatctggc
    27061 acagggaata catttaatga attgacgtat tctgcattta cttgcagacg attcgcacct
    27121 gcctggacca tttcctggaa ctggcttgca cttatttctg ttacactcac ctagataatc
    27181 ctccttcaga tcgactgctg agttaccagc cagtctcctg cctagtctgg cagtacacga
    27241 acaccttgta caaagacatt aactgaattg gcagttacgc ctaataatgc ctctagattg
    27301 tatttcactt tttcttggac gttgcgggaa acttcagaga ttttcgtacc atagctgata
    27361 atcgtataaa catctacagc aataccatta tcttcttggc ggacaactac tcctcttgag
    27421 taattttctt tacgcaaaat ctcagtaata ttatctttaa tttgattttt gctggccatt
    27481 cctacaacac cgaacacatc tgtcgcggcg ccaccaacaa ctgttgcaat tacatcattg
    27541 gtaatttcaa tggttccagc tgacgtttga atttttacag ccatgaatga gcctccttaa
    27601 cgggtaaaaa actcccattt ttcttctatc atattttatc atagcgcaac ttgaaataaa
    27661 aggcagttac ccaaagtact caaaaacctt gcaaaatttc aggtataacc ttgtttaatt
    27721 tacaattatt cttctcaatt gtcaattgta tctatgaaaa tgtacttgca aattcccatt
    27781 ctatatgata tgttagtctg gtatgagctg aattattcta ttgctccaaa tcaaggcaaa
    27841 ggaggaaatt accatggcaa aagtttgtta ctttacaggt cgcaagacaa aaagtggaaa
    27901 caaccgttca cacgctatga actcaacaaa acgtactgtt aaacctaact tacaaaaagt
    27961 tcgcgttatg gtcgacggaa aacctaaaaa agtttgggtg tcaactcgtg ctttgaaatc
    28021 aggaaaagtt gaacgcgttt aattgaatga tacttggacc gttcagatag tgaacggttt
    28081 tttatttaat aaaaattttt aagggtggga cttttgattc ctaccctttt tttgcgttta
    28141 tttt
//
LOCUS       DACCWK010000026        28115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCWK010000026 DACCWK010000000
VERSION     DACCWK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.026"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(44..1612)
                     /locus_tag="IVW65_001090"
     CDS             complement(44..1612)
                     /locus_tag="IVW65_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7900005.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1616..2026)
                     /locus_tag="IVW65_001091"
     CDS             complement(1616..2026)
                     /locus_tag="IVW65_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7900006.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2023..2946)
                     /locus_tag="IVW65_001092"
     CDS             complement(2023..2946)
                     /locus_tag="IVW65_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7900007.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2943..3821)
                     /locus_tag="IVW65_001093"
     CDS             complement(2943..3821)
                     /locus_tag="IVW65_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7900008.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3808..5145)
                     /locus_tag="IVW65_001094"
     CDS             complement(3808..5145)
                     /locus_tag="IVW65_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7900009.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5148..6251)
                     /locus_tag="IVW65_001095"
     CDS             complement(5148..6251)
                     /locus_tag="IVW65_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7900010.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6325..7833)
                     /locus_tag="IVW65_001096"
     CDS             complement(6325..7833)
                     /locus_tag="IVW65_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7900011.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7799..8491)
                     /locus_tag="IVW65_001097"
     CDS             complement(7799..8491)
                     /locus_tag="IVW65_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7900012.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8513..9487)
                     /locus_tag="IVW65_001098"
     CDS             complement(8513..9487)
                     /locus_tag="IVW65_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7900013.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9787..10659
                     /locus_tag="IVW65_001099"
     CDS             9787..10659
                     /locus_tag="IVW65_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7900014.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11117..13315)
                     /locus_tag="IVW65_001100"
     CDS             complement(11117..13315)
                     /locus_tag="IVW65_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7900015.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13547..14539
                     /locus_tag="IVW65_001101"
     CDS             13547..14539
                     /locus_tag="IVW65_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7900016.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14599..16020
                     /locus_tag="IVW65_001102"
     CDS             14599..16020
                     /locus_tag="IVW65_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7900017.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16013..17512
                     /locus_tag="IVW65_001103"
     CDS             16013..17512
                     /locus_tag="IVW65_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7900018.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17551..18945
                     /gene="uxaC"
                     /locus_tag="IVW65_001104"
     CDS             17551..18945
                     /gene="uxaC"
                     /locus_tag="IVW65_001104"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7900019.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19104..19787
                     /locus_tag="IVW65_001105"
     CDS             19104..19787
                     /locus_tag="IVW65_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7900020.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19789..20625
                     /locus_tag="IVW65_001106"
     CDS             19789..20625
                     /locus_tag="IVW65_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7900021.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20636..22390
                     /locus_tag="IVW65_001107"
     CDS             20636..22390
                     /locus_tag="IVW65_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900022.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22666..23007)
                     /locus_tag="IVW65_001108"
     CDS             complement(22666..23007)
                     /locus_tag="IVW65_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7900023.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23075..25246)
                     /locus_tag="IVW65_001109"
     CDS             complement(23075..25246)
                     /locus_tag="IVW65_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7900024.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25457..26314
                     /locus_tag="IVW65_001110"
     CDS             25457..26314
                     /locus_tag="IVW65_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7900025.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26385..27185)
                     /locus_tag="IVW65_001111"
     CDS             complement(26385..27185)
                     /locus_tag="IVW65_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7900026.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(27182..28084)
                     /locus_tag="IVW65_001112"
     CDS             complement(27182..28084)
                     /locus_tag="IVW65_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7900027.1"
                     /translation="MNQSVAKTAHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKS
                     NCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSD
                     LPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVIT
                     LVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNV
                     NDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRK
                     SLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         8353 a         5783 c         5042 g         8937 t
ORIGIN      
        1 tagtgaaaat aagttagtga acctcattta ttaaagaatt aaattacaga tgatcttttc
       61 catcatactt gaaaaactca aaatcagcat aacttcctct ttttttattt agatcatgca
      121 ctgctatccc gataaaattt ccagtaaacc ctcctgagag tatacacatg tttttttgcg
      181 gacctacttt cttccattct tcagttgata cgtctttcaa atgaaactgt gctgtctctt
      241 tctttacagt gactttcaaa aaagtcttct tttctgtgag tggaatcgtc caatcttcta
      301 tttgtgctac tccgttttct gattctatca tacgtaggca ttttcccttg tcctcattcc
      361 aagtaacata acaataaata taattggatt catttaagta cagtaccagt ccagccatct
      421 gattaaaatt attaggcatg tattcaaatg ctgtctccgc atcaaaagta aaatccgttt
      481 gccgaatagc taacaaatga tgttcgaagg tagattgaag agattcaccg gaaatcagac
      541 gtaaaaaacc tggtctacat tttgtatcac accaatcagc atttggcaaa atgcgtctgg
      601 cattccagtt cttctttagc tctccttcaa atgtatccat aaactgatga tctttttctt
      661 gttgtacctc ttcatttgtc tcaatcatta tttcttccat cggagttgtc tggctatctt
      721 taagtcttaa ccaatcgtcg gacgtccaat acacttcttg aatagcggtt tctcttccca
      781 aaatagccgc accgtttaat ggtctggtac aaagatatgt catgtaccat cttcctgacg
      841 gggtttgtac taagctccca tgtcctgtac attgcaatgt agaagcaggt ttgtcactag
      901 ctgtcagcat aggaaaacca ggatcaagct cgtaaggacc agtaatattc tttgctcggc
      961 aaactgtaac tgaatgtcct cttcctgtac cgccttctgc cgtcatcaaa taataatacc
     1021 cgttacgaaa atacaaatgt ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt
     1081 tatagatggg accatcaagc ttcttcttct ctgcatcata ttcttgtaat acgataccgc
     1141 tggatttatt cggtgtgtcc agacgataat cccatagttc attgacaagc catttttttc
     1201 ccgtttcaag atcatggaac aaagaaggat caaagccgtt actatttaaa taaattggtt
     1261 cggaccaagg acctgtgatc tcagatgcag tgatcaaata attgtggaca tctttaaacg
     1321 gacggcctgc acttttgaca tctgtgtaaa caagataaaa caatccatca gcataactga
     1381 tctgtggcgc ccatatactt ccatttgtag gatttccttg caaagatact tgagtggtca
     1441 aaatatccgt tgtatgtgac cagttgacta aatttttcga ctcatatact cggattcctg
     1501 gaagccattc aaaagaggag acaataatgt aataccgctc tttcactctt aatatattag
     1561 gatctggatt gaatcctctt aaaataggat ttttcacttt gatagtctgc atgattcagg
     1621 ctccttttct cattcttact cgatataaac tgaatgcccc agctgcgaaa taaaaaaatg
     1681 caaaaggaaa cgcaataaac tgagtgccaa tcaacaatat gaatcctgtt ataaaataca
     1741 aaccagtggc agttgttaag tattttcctt tagcagtcaa aaacaatgct actgctaaaa
     1801 aagcaagtga aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa
     1861 atgtattttc taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa
     1921 actctgacgc agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata
     1981 gactgccacc gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg
     2041 cttttctaaa tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc
     2101 agagaatttg acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg
     2161 aacatgccca cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct
     2221 tcctaaatag acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata
     2281 ccctgttttt tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc
     2341 taagctatgg atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac
     2401 cgttccttca atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc
     2461 cggtgtacaa aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt
     2521 atcctgataa ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa
     2581 agcaacagcc tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc
     2641 taatacgaca ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct
     2701 accgtcaaga tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc
     2761 cacaagttta aaattggata atctgctttc tatatattca tcatatcttc cttcttttac
     2821 tatcaagacc tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat
     2881 agtttcaaaa tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat
     2941 cctcataaat gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata
     3001 tgaccgcctg ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt
     3061 ttgtccagcc cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt
     3121 ccaactaagc taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa
     3181 gctgtcaata gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc
     3241 aaaagtattt cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc
     3301 aatgctgtca cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt
     3361 tgagtatctg tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata
     3421 cgcattcccc ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa
     3481 atacctccgc gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct
     3541 gtcagtactt ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt
     3601 ttcccgactg aaaaatctcc gccgtgagaa tgcaggtaaa ggaccggcgg aaacggtcct
     3661 tctttttctg gataagcata gagggctggt actgtttcca attcatttaa gtacaattga
     3721 tagtattcaa gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta
     3781 tgtctgctag atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac
     3841 aaccagttca tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt
     3901 agtattttct aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga
     3961 aaccttaccc gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat
     4021 catagcagga acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt
     4081 gatttcttgt atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt
     4141 ccttgccgtg atattcgaaa aatggatcct tctgaatgct ggtgtccgtt catctatcgg
     4201 ataagccttt ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag
     4261 attcagtata aatggacaca tcacattatc catgactata ttggatactc gaatatcttc
     4321 aacgatcccg cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa
     4381 gatacaattt gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac
     4441 accgccatgt ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct
     4501 ttcgtacgta tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg
     4561 gcaattacta atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga
     4621 atccgctggg ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt
     4681 gatcgtccaa cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt
     4741 atggaaactc atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt
     4801 atgccaccat ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc
     4861 tacattttga gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga
     4921 atgaacaaca ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa
     4981 atgaagttcg acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac
     5041 aactacggtc ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt
     5101 attcaattca tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc
     5161 acttcaatca atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt
     5221 tcactgatgt agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact
     5281 tgacagtttt tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt
     5341 aatccttgat gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa
     5401 cgtattcctt tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt
     5461 cctttccgtt ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat
     5521 ggaagtaatg cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct
     5581 tcatgaagta attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc
     5641 caaaaatgat gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc
     5701 catgcatgaa tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac
     5761 tgtctcaaaa catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa
     5821 aaaggagaac ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc
     5881 caaaaagctc cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct
     5941 gcttttttat atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta
     6001 tttacatggt cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc
     6061 acgaaatagt cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc
     6121 caaagtaatt ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac
     6181 cacttttctt catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac
     6241 cagttttcca ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag
     6301 aagtatttgt cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc
     6361 ttgtgtgatt cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc
     6421 ttcgtcaagc tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc
     6481 tgatcctact tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa
     6541 ttcttcattt tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa
     6601 agagaacgtc acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag
     6661 atccatatcg ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat
     6721 accgccagtc atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa
     6781 tttcttcaat tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat
     6841 accatttcct tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac
     6901 aggcactaat tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc
     6961 caccattcca gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt
     7021 ttgggtaact gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt
     7081 caaagctttc atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac
     7141 agcccagcca tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat
     7201 atcactaggc gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc
     7261 gttttctgta aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag
     7321 tcctagatta tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg
     7381 tatcggtacg ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat
     7441 ttgacttaaa tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt
     7501 taacgcattg cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc
     7561 tagttcatca gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca
     7621 gttgaatgtc agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg
     7681 cggtgaagca tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc
     7741 tgaagaactg ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact
     7801 aagacctaat tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa
     7861 tatttttgtt ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata
     7921 tagaatttgc tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa
     7981 cacctggcat tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta
     8041 atgagtaatg aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc
     8101 ctttccaatc ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga
     8161 acgcagcaat atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa
     8221 gtaaaacaat cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt
     8281 ctttaaaata tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt
     8341 ttctaatcac tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg
     8401 cgatgactaa accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc
     8461 ttccgtttat tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa
     8521 cgccgtcttc tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta
     8581 cgaccccttt aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag
     8641 ctgtataaat ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca
     8701 agtacatatg ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa
     8761 tgattactgg acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc
     8821 catccatttc tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga
     8881 tgatcgtaga ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc
     8941 attcagggga tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca
     9001 atacccctcc gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca
     9061 tgaagtgagg gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt
     9121 catttaatag taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga
     9181 acaagatcaa cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga
     9241 aatgtttgaa accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa
     9301 aagcaataac gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg
     9361 gcagaatcat cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc
     9421 ttctgagctt ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac
     9481 tttccataag cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata
     9541 actatctgaa ttacgtatat aatcattttt accgtaagta aaaacgctta catagcaatg
     9601 atttttgtgc tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa
     9661 accttctatt tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc
     9721 accgatgata aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct
     9781 atctatatga aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg
     9841 tttctaattt tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct
     9901 tttgcttcta cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct
     9961 ttagacgcgt atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg
    10021 attggtgtta caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac
    10081 ggtctttcca gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct
    10141 atgttgttca gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc
    10201 aattcttact gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg
    10261 agaaactttt tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc
    10321 acagattttg gtgtattttt caaaattatg ctgccattag cgttaccgtc tattgctact
    10381 atctcgcttt tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc
    10441 aacgattcat ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt
    10501 gggatgcaag cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg
    10561 gctgttatcg ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat
    10621 tttgtcaaag gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg
    10681 gtagttgtgt cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc
    10741 ttaaattttt gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta
    10801 tcagaggttg tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg
    10861 cgacgagtaa cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca
    10921 caaaattatg ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga
    10981 actgtgacaa gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc
    11041 cataaataca aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga
    11101 aaaacagcct cttgattcac aaatattttt ttcgatattc tcccggcgta atcgattcct
    11161 tctttttaaa gaaacgaata aaattttgcg gatttcgata ttgcaatctt tctgcaatct
    11221 cttttacgga gaggttagtc tctttcaacc atttttttgc tacctctaaa cgatgatttt
    11281 gaacaaaatc accaaatttt tctcctgttt cctttttaaa tacattactt aaatagttag
    11341 ggttataatg caacctatca gcgatcacat ctaaagacag atcacgatca tattctgtgt
    11401 agacgatatg cataatattc tccgacaaat ttttaaattc atgatcagtg attgcttgag
    11461 agcattctgt gattggcaga actaattgtt ctaaaacaag tttttctagt tctttgggat
    11521 gataattgtt cagtgctttt acataaattt gtttcatgtt atcaaagttt tttgaatcta
    11581 ctcccaataa ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa actacttccc
    11641 gactcaaagg atttttattt tgtgcaaaca atgcatcaat caattcatgt actaaattct
    11701 tcacttcctg atttccagaa cgaatggctt caaacaatcg attttgttct tctatcggat
    11761 atctaataag tgttttttca tgagcagcag gaacgatttc ctgataaaaa ataattgaac
    11821 ttggaccagt atttacacga taatacaggg cttcttttgc ccgatcaacc gattgcttgc
    11881 tttcattcag cgtttcgaac cggttactga tgcctatact gatagttaaa ttcaagtatt
    11941 cttttacacg tttttgaatg agtcggcaat actccatgac ttttttgctt gcttctgcat
    12001 catctttatc cattcgataa atagtggctt gcatttcttc attcaaaacg atcggcagca
    12061 ttcgattttc ctttggcaca atttccgtga tcatgttgtt gatcgctaaa agtagcaaat
    12121 cacgctcccc accgtgacta tcttctaaca gatcgatttg aaccaaagct gtataatagc
    12181 agtcattttg agaactataa ccaaattgct gcaaccgctg atctagttct cgcctttcta
    12241 ctcgatttct aaataaacta agtacaaata gcgtttccag ctgtggcttt tgggtaacta
    12301 agtgtgcact taacgcttct ttttctccta tgatccgact gactgattga gcgaccaaat
    12361 ctagttcttt cccactgaat ccttttgatt taagattcag acgttcctgt atttgagaaa
    12421 tcggacgggc aaatctgtcc gaaaataagt aggaaagaag accaatgagt cctaccagtg
    12481 ttaaagacac tgcaactagc ccgattctca gattgcggat cattgcgcca attgcactag
    12541 agtcaacttc taatctgtaa atccaattat tataatctga tttcagtact atatacgttt
    12601 tatctttttc tcttatcact ttcagctgat tgtaatctag atccacgtcc gttaatacag
    12661 ttggttgctc tttatttttt actgtctgag tagtaaaaag gttgccaact tcgttttcaa
    12721 tagaaagtat gtcttctcca ctttcaccaa caacttgttc tattgtatgt ttctctatcg
    12781 tagcaattcc caatgcatat ttttcttttt caaacattgg caaagaaatc accatttcga
    12841 ttcctttgtt tttcttttgc caaaataaat tatttttgtt atttatgtac ttctgttgat
    12901 acttttcaat ttcctcttcg gtcagttgac tcaacgatcc attgattact ccccatttac
    12961 cagccaaact gactaaatca taattattat catccataac aatcgtttct ataaaattta
    13021 gttctttcct gatttcagaa taagtggagt aatctgtata tgtcagcgga tgcttgaata
    13081 tgtccgagaa atttttggtc gaaccatacg tagaaaacga atattcaatc gttctaattt
    13141 tccattcaac gttagtttta acttgcctga gcaaattctt tttgtctgtt aaataactcg
    13201 attcgatcga ttcataggta ttctggtaaa tgaataaact aaatcctatg acaagtacgg
    13261 ctcctaataa aaacatcggt aaaaatattt tataaaatac tgtacttttc ttcattttct
    13321 tcctctcctc cttttaaaat aaaaggcttt catttttctg taaaattgat actcatctaa
    13381 ttaaatgagc attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt
    13441 tttaacgtgt gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata
    13501 tgatgaatgc gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa
    13561 agatgtcgct aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag
    13621 cagtttaata aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta
    13681 ttcgccaaac tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt
    13741 tttctcaagt ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat
    13801 ccattcagta ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt
    13861 aacagaaatc aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga
    13921 tcagcctttc atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca
    13981 catcaatgat ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc
    14041 agtagattac ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa
    14101 ttttcgttcg acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat
    14161 ccctgtcaat ccagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa
    14221 catggaatac ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga
    14281 catagcgatt ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat
    14341 ttctctgatc gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac
    14401 cgtacgtaag cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat
    14461 cgaaggcagg acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga
    14521 gtcgatcaat tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa
    14581 cacagaaagg aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca
    14641 cctgtaaaag ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag
    14701 attcagcagc tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta
    14761 gaacaaggtc ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa
    14821 ggactcagca atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg
    14881 atcaatcctt atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc
    14941 tatatcgtct ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca
    15001 gatactcccc cacagagcct cccgggcaaa ctgacagcct ttttataccg acgtttcaaa
    15061 ttaaacaaag ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt
    15121 ctaaaagaaa tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag
    15181 tggttagaaa aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat
    15241 cctcgcgaag atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc
    15301 aaagcagaac cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta
    15361 ccattcaaac aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa
    15421 cgtaaggtgc atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg
    15481 gaattggaaa ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag
    15541 ttattcaatc tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat
    15601 gctgaccaaa tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt
    15661 tcattaaata gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc
    15721 gaagaacaaa atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg
    15781 tatcgaggcg atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat
    15841 gcttggtctg ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga
    15901 gaagatttgt ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc
    15961 gatcaaaaag gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga
    16021 aaccctgcaa ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc
    16081 tattccaaaa caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat
    16141 cccgcaaggt cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata
    16201 tggctatcca atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca
    16261 taatgtaaaa accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc
    16321 aagtcattac ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt
    16381 agggattcgt aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga
    16441 actgatcgtc aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac
    16501 gattttaaag catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga
    16561 aattttagcg gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg
    16621 ttgtgagaac aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg
    16681 agtgaaattt ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt
    16741 agaagagctt ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt
    16801 gaatgtcggt ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt
    16861 acttggtgca ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt
    16921 gccagaaatg tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt
    16981 tgactcaatc gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc
    17041 agtttacgaa aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa
    17101 gtctttaggc tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg
    17161 cgaaaaaatc agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc
    17221 tgcctctgct cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac
    17281 tccgtttggc tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg
    17341 caaaggtcat tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat
    17401 cttagaatcg tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga
    17461 acaaaatgac gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata
    17521 aaacagtata aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa
    17581 gacgattggg cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat
    17641 cactgtcact tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa
    17701 gcttggttag gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa
    17761 gaaaaaatca caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta
    17821 ccaaaaatta tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc
    17881 ggtattgact tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa
    17941 attttggcag ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg
    18001 atttgtacaa cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa
    18061 gaaccagatt tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa
    18121 gaaggattta atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc
    18181 tatcaagaac tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt
    18241 cgtgtttccg accacgcttt agacatctta cgctataaag aagcggatga aacagtatta
    18301 gaagagattt ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct
    18361 tatcgtacag aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg
    18421 cagctgcaca tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc
    18481 gataccggct atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg
    18541 aaccgtgcag aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat
    18601 gactatcctg cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg
    18661 cagttaggtt ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca
    18721 caatttgctg atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt
    18781 ttcctgtcct acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa
    18841 atggtcatgc gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg
    18901 attagttata caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac
    18961 agtaacgaat caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga
    19021 agaagacgga ccaaatttgc tccgtcttct tctcttgctg ttctgcttca ttgcctataa
    19081 acctttatga aagaagagat aacatgccta ctatccatat tgccggtgat tctactgcgg
    19141 cagaaaaaga tgaaaacaag cgtcccgaga caggttgggg agaaaaaatc tctgcttatt
    19201 tttcacccga gatccagatc aataaccaag caaagaatgg acgcagcacc aaatcattta
    19261 tccaggaagg aaggttagcc cgtctagctt cacagtttca gccgaatgat ttcttatttg
    19321 tacaatttgg acataacgac cagaaaatcc aagaagaaag aggtacgctt cccttcggta
    19381 gctatttaaa aaatctgcag atattttttg aatgcgcaaa aaaagcgaac gtccaaatgg
    19441 tcttactgac atctgtgacg cgacgagatt acttagaaaa cggaacattg aatccagata
    19501 tacttggaga ctatccaaaa gcgatgcgtg catttgctga gaaacaccat attcctcttt
    19561 tagatgtgtt ctcaagatca caagaattgt tccagacctt ctcaaaagaa gagacaaaaa
    19621 aattctacct gcatcttata cctgatactt gtaaaaatta cccagaagga ctaaaagaca
    19681 atacccactt tagtccagaa ggcgctgata aagttgcccg aataattgtt gaattgataa
    19741 aagaatcccg tctgcctctt gctaattatt tacaaaaagg agtttgagat gtttccatta
    19801 aatttaggca tccgtgccca cgatttagat gttcgaaatc gtgaagaaat cgttcaaaaa
    19861 gtatcacagc ttcacttgtc ccatatccaa tttgcaccac aaaaagcatt tcctgaacaa
    19921 cttaaattaa ctgacatgac acttgggagt gctgcctatt ttggtgaata cttccaaaaa
    19981 aacaatatcg aactttccat tttaggatgc tatatcaacc tatccagcaa aaatcaagaa
    20041 gttcgtgcac aagcattggc aactttcaaa cagcaaattg ctctgtgctc tgcctatcaa
    20101 gccaagatga ttgctacgga aaccggtagt gtctctgtcg gttacacaaa agaaaatttt
    20161 actgaagaag cttatcagat cgccagaaat tctatcattg aattagtcgc tcatgcagaa
    20221 aattttggcg taactgttgc tgttgaagca gggatcaatc atccaatcta taactatcag
    20281 ttagctaaac gtttaatcga tgaagtagct tcaccaaatt taaaaatcat tttagattgt
    20341 gcgaatttga tgcataaaga aaatcatgct gatcaggaaa aagtgatccg aggtgccttg
    20401 gacaatttac atggttatat tacagcgctt catctcaaag actatatcat ggaaaatgga
    20461 aagatccgca tggtacctgt tggcaagggc tgtttagatt tccgcccggt actgcattat
    20521 atcaaatatg agcgtcctct aatgtacgcc actcttgaag ctactcctga gatttatgtt
    20581 cctgaagcaa tagaacactt gcagtttatt taccaagatt gttaaggaga agattatgga
    20641 ggaacgaata cagaaaagtt atcaaaaata tcatgcaaat gcttttcaga cattgctgca
    20701 tctttatcag ggaaattatt ggcgttttgc catgtcagga ctctttttta ttatcaaaca
    20761 tcttccatct tggttaatgc cgatcgctat tgccaatgta atcaatgcag cttcatcgaa
    20821 agattcttct cagttgcagg ccatttattt caacgctttt ttgatggctt gtctgattgg
    20881 tcaaaatata ttgacgaatt attttcatgt aaaataccat agcacttcga tccgtcaagt
    20941 agaagctgga ctaagagcga cccttatcaa gaaaatccaa gaactatcta ttccttatca
    21001 aaaagaattg caatctggac gaatccagtc aaagatcatc cgtgatgtag aagctatcca
    21061 aacgttgtcc tctcaagtat ttgtcagcgg attaaatatc gctgtcaatt tattcgtagc
    21121 tttaggtatc acgctttata aaagtcctgt tgttttcatg tttttcttat tgactgttcc
    21181 tctcgccgca ttcgttactc tctttttccg taaaaggatc agacagtcca actcagactt
    21241 caggcaagaa atggaagaga cttctgcaaa agtgatcgaa gtgatcgaat tagcaccgat
    21301 tgctcgagct catgggttag aaaaacacga aacaactcgc ttaaataagc attttactca
    21361 aatctatgaa aaaggattcc acttagatat cctgcaatct gttttcggat cagtcagctg
    21421 ggctagtttt cagatatttc agctgatttg tttgattttc actgggatat tagctttaca
    21481 agggcgcatc caacccggcg atgtcgtgat gtatcagacg tatttcacga cgatcgtcaa
    21541 ttccgtttct ggaatgatca cgctgattcc tacgatttca aaaggcatgg aatctatcac
    21601 ttctattggc gaactgttga ttgctggcga tgtagaaact tatcacggta agaaaaaaat
    21661 ccaacatttg gatggaaatt ttcgttttga ggatgtttct tttctttatc cggactcact
    21721 caaaccgatt atcgatcatt tttctctaga ggtgaaagct ggagaaacaa ttgcttttgt
    21781 tgggccatct ggctctggaa aatctacact ccttaactta ttgattggat ttatccagcc
    21841 gacttcagga aaaatttttc tagatgatca gcctttggat gaactggata tgcgaagtgt
    21901 gcgaaattat ttagccgttg ttcctcaaac cactttgttg ttctccgctt cgatcaaaga
    21961 gaacatcact tatggcttga aaaacgtatc aaaagaacgt ttagatgaag tcatcgaagc
    22021 tgcgcaactt tcttcactga ttgaccagct tcctgatggt ctggatacct tagtaggcga
    22081 acatggcaac aagctgtctg gtggccaaaa gcaacgtatc tccattgcac gagcgttgat
    22141 acgtcagcca aaaatcattt tgctagatga agcaacctca gctttagata accagtcaga
    22201 gaaaaaaatc cagcaagccc tgaattacct gacagaaaca cccactactt ttattgtggc
    22261 tcatcggctt tcaacgatca aagaggcaga taaaatcgtt gtgatcgatg aaggaaaaat
    22321 cgttgaaata ggtacttacg aaaaactgtt agctcaaaaa ggctatttct accgcttgaa
    22381 aaatgaatga caaaaaatag gagctatgac aagacttttg tcgtagctcc tatttctgaa
    22441 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatagtcatc aaacatgaga
    22501 agctgttttt tgaaattgtt ccttgtaact cagagttaaa cggtaggctc acgacctctt
    22561 ggtaaatggt gttcaagaga cagcctatcg gcaataagaa gcaattttgt catcgctcaa
    22621 ggccgaacgt ctctttcaca ccctctgttg tttcataaaa tagttttatt cgctgtaaag
    22681 gtcgcgaact ggtgtcatga tgatacggtt caagtcattg atgattgtac tgaagccttg
    22741 ctctttttgc atcaagtcat tgattacttc ttctgattgg attttcacag ccatttcttg
    22801 tgctttcatt gcatcttcgt cgctgaactc ttgtccactc atttgttttt cttgtaattc
    22861 gatttgcatc gcttggaagt ctttgaacag tttgtgtgct tcttcgtttg cttgtacttt
    22921 ctcgtacgct tctttcaacg ctttgaattc tggtaattca cggatctcac gttcgatttg
    22981 attcgcgcta tcgtagatgt ttgacatgtt atgccctctt ttcttttagt agttactctc
    23041 tattgtaaca tggggttatt cgttttcaaa ggggctactc tccttgatat ttccgccaga
    23101 ggtctttagc cgcttcacca attccctgaa cgccttcttt caccatatcg ccgaagtttt
    23161 tcagcccttc ttttgcttgt tcaccatatt gattcaagtt atcttgccaa tttccagact
    23221 ctccattaga atttcctgtg tttccaactt gatctgcagc taccacttgt cctcctgtgg
    23281 cataggcatc tgctacacta aatcctgttt ttgaagtata cggaaggatg ccttctgccg
    23341 ctgatttgaa gactttcccg acaacatttc cacttgtccc ttccaaatag tgcagcttgc
    23401 tggattcttc gtaacccatc catgtcgaaa tcactacgtc tggcgtgtaa ccgatcatcc
    23461 attggtcatt tgcttttgtg gcatcgaaat tagtttctgt ggttcctgtt ttccctgcta
    23521 tcgtataacc tgcaggattc gcagcagccg ctgttccatt tgaaaatgta ccaagaagca
    23581 tgctggtcat ttcatcggct gtttcctttg atatgacctg ttttggtttt ttgtttgtgt
    23641 tatcgacgat caccgctcca gtcgaatcaa cgattttcgt gataagatgc ggcgtataca
    23701 tctgaccatc attggcaaat gcactgtaag cacccgccat gatcaatgga gatactcctt
    23761 tttccagtcc accaagggct aagccataat acttatcaga ttttgctaaa ggcaggccga
    23821 attcttctgc tttatcatat cctttttgta aaccgatctc atgcaaaagc catacagtag
    23881 gcaaattcaa actttgtgca acagcttgat acatcggtac ttctccttgg tatgtcccgt
    23941 caaaattatg cgcatcgtaa taactttgcg gttcgtcttt caagatactg gaaggcttgt
    24001 atccagcttc cagtgctggt gtatatacgc ttaatggttt gatcgttgat cctggtgaac
    24061 gacgcatctg tgtagcaaag ctgaatcctc taaagacatg atctcctctt cgtcctacta
    24121 gtgcttgtac accacctgta ttcggatcta aagctactga agcagattgg accatggcac
    24181 catctgaagc atttgctggg aataatgcac tgttttgata agtagcttct aattgctttt
    24241 gataatcctg atctaaagct gtatagattt tatagccttt attcatgaca tcttcttcat
    24301 caagaccata ctcgttaact gcttcatcga tcactgcatc aaaataataa ggataacgat
    24361 aaccagaatc tgaattatcg tatgtgtcat taagatacgc gctgatatct gtctggcttt
    24421 ctgcttgttc ttgttctttt gtgatttttc cgttttcttc cattacactt agtactgtgt
    24481 ttttccgatc attcgcattt tttgggtgat cgatcggatt ataaatacca ggacctttca
    24541 gcattcctgc caaagtagca gcctcagaca gcgtaacatt cgttgcatct actccaaaat
    24601 actttctgga agcgtcttgg acaccccata ccccgttgcc aaaataggca ttgttaagat
    24661 acatggtcaa gatttcttct ttgctgtact tcttttcgat ctcgattgcc aaaaataatt
    24721 ccttcgcttt tcgatcaaac gtttggtcta atgtcagata cgcatttttg gcaagctgct
    24781 gagtgatcgt acttcctccg ccccctgatg ttccgccaga caagaccatt cggactgctg
    24841 cacgggcaat ccctttgata tcaaacccgt gatgctcgta aaaattccta tcttctgtcg
    24901 agattactgc atcttggata tagggagaaa tcgcgtttaa ttctacatat gttccttttt
    24961 gggcgtacag tgtgccagct tcttcgtttt tcttatcata aatgactgtc gattggctta
    25021 atcctgattt taacgtttcg acatttgctt gttttgctaa aataaagagg tagatacttg
    25081 tcactaagac acagatcaaa ccaatcagca aaatcaattt gttcacatga tattttttcc
    25141 agatgcgctt tcgccatcga tggaattgtc cgagatatgg tctgatccaa cgccaaaaac
    25201 tgatcaaagc agcttttatc ttttctaaaa tgctttgaaa atccatttgc ttcttctcct
    25261 gtctatttga ttcaatcgtc ttattttacc atagtgaaag agtgttttct tcctgcttaa
    25321 gttgtagttc aatatgtttt tttacgttag tgcaagtaga gtaacataag aatgaacaga
    25381 aaaatgtaag ttttttctta aatatgtttt aaaaatggta caattagtct gcgattgcac
    25441 tgaaaggagt acacttgtga aaatcacgat cactttacct atgaaccatc ctgaaacaac
    25501 aattcgcgac ttgcttgaaa aggaatggtt agttcctaga aaggttcgac actttttgcg
    25561 tatacgtaaa aacgtttgga tcaatcaaca acctgctttg ttccatcatg aagtaaaggg
    25621 caatgaccag atcacattgt tattcgaaga aacagattat aattatcaag aagttcaatt
    25681 aggagatgcg aagcacatcc gtgttttata tgaagatgag catttgatta tagtggataa
    25741 gcctgcagga atgaagactc atccaaatga accgacagaa aacagtacct tactcaatca
    25801 tttagctgct tatctcgaca ctaaacagca acgaccttac gttgtccacc gcttggacaa
    25861 agaaacaagc ggtgccgttt tatttgcaaa aaatccattt gtcttgccga tacttggaag
    25921 aatgttggaa caaaaaatga tctatcggag atatcaagcc atcgtatggg gagaaattgc
    25981 caaagatatg accatcacca ataaaattgg acgagatcgc catgatcgga gaaaaagggt
    26041 cgtagatgat aaaaatggtc agagcgcctt gacacatgtc actgtcaaca aagtactttc
    26101 tgaacaaaca caagtctatt gtgttttgga aaccggacgt acacaccaga tcagagttca
    26161 tttagctcat aagggacatc cgatcgttgg agaccctctc taccaaaata gaacagctga
    26221 acgactgatg ctccatgcat tagaactgca catgatccat ccatttactc aagaaaaaat
    26281 agtcggtaaa gctttacctg gattatggga atagaaataa aaaagaacag agtcttatca
    26341 gcagataatg ataagactct gttctttttg tgtaatttgt ctgcttactt ttgatgttcg
    26401 tgttctgcta acagcaaact accgataatc ccactttcat tcggcagtcc ccaacgaacg
    26461 atatattcac ttacttgagg tgtttccata taaccaccca tcaattcaac aaattgctga
    26521 cggatttttt gaaggagatg atcctgattc attaccccac cgcctaaaat aatcttttca
    26581 ggtgctaacg tcaacgtata gttgatcagt gcttgagcaa tataataagc ctgaatagcc
    26641 caaacaggat gatcttgcgg cagatcttgc cctttgatcc ctgtgcgtgc ctcaatagac
    26701 ggtcctgctg ctaatccttc taaacaatct ttatgataag gacaagtacc ttcataatgg
    26761 tcatctggat ggcgtttcac ccaaatatgt cccatttcag gatgtgcgat ccctgaaaaa
    26821 atttcttcat tgatcacgac acctgcaccg attcctgttc ccacggtaag gtaaatacaa
    26881 ctattttttc cttgtgctgc tccttgcttc aattccccat atgcagcagc attgacatcc
    26941 gttgtccaag cagcaggaat attgtagcgt tccttgattg tgccaagaaa atcgaattgt
    27001 gcccaccctt ttttgggagt tgccaaaacg tatccgtaat tatcttttgt tttgtcgata
    27061 ccaatcgggc cgaatgagcc aatcccgatt cccttcaatt cataacgatc aaaaaattca
    27121 aatacctgat ccaatgtttc ttctggtgtt gtggtaggga tacttaattt tttcttaatt
    27181 gtcaaattaa gttagacaga tcatcaatac ttacgtaact caaaaatact tccaaaggtg
    27241 ttcgataatc cagtgatttt ctaggaatat tatttcgttt agatgcgaca gattgaataa
    27301 aaaattcatc tactgaattg aaatccatag atttcaataa accatctcta cgtaacaatc
    27361 cgttagaatt ctcgtttagg cctctttgag acggcgttcc tggatcagcg aaataaatgg
    27421 caatatcatt gacattactg atctgtttcc aatttgaaaa ttcctttcca caatcaaaag
    27481 tgatggattt gaataggttt ttcggtacag attcaaacca ttgatttaat tttttttcaa
    27541 tatcaatcgc ttgtctacca cacggtttca atgtgatgat aacttttgat aatcgttcaa
    27601 ctaaggtaat tacagcactt ttatgtttca gacctacgat agtgtcacct tcaaggtgac
    27661 caaactcatt tgagaattgg ctataatcct tttcacgttc atgaatagag cggcggaaag
    27721 tttgttttcc acgtctttct tgatgtccat tcggtttacg cttgcctttc atcggtaagt
    27781 cagaagaatc aaatagcccc tttttgaaca tacgataaat ggtacgagca gaacaactaa
    27841 taggaaacgc tgcacgacca acaatcacat cgggtgtcca tccttgaaca acctttcttt
    27901 gaatatattc tgattgttcc tcgggtaaaa caagcggacg tctaccacag tttgatttgt
    27961 ttttcttata ctgttgataa tattctaagg ctgattttcc ttgcttgaag aacagatata
    28021 ctttatggat cgtttgtctt gaacgattca agcaatgggc agttttagca acagattgat
    28081 tcattttgaa ataagactct attattacaa gttca
//
LOCUS       DACCWK010000027        28091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCWK010000027 DACCWK010000000
VERSION     DACCWK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.027"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(39..863)
                     /locus_tag="IVW65_001113"
     CDS             complement(39..863)
                     /locus_tag="IVW65_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7900028.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(836..1516)
                     /locus_tag="IVW65_001114"
     CDS             complement(836..1516)
                     /locus_tag="IVW65_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7900029.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1539..2477)
                     /locus_tag="IVW65_001115"
     CDS             complement(1539..2477)
                     /locus_tag="IVW65_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7900030.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2722..3573)
                     /locus_tag="IVW65_001116"
     CDS             complement(2722..3573)
                     /locus_tag="IVW65_001116"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7900031.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3727..6300)
                     /gene="mprF"
                     /locus_tag="IVW65_001117"
     CDS             complement(3727..6300)
                     /gene="mprF"
                     /locus_tag="IVW65_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7900032.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6441..7247
                     /gene="proC"
                     /locus_tag="IVW65_001118"
     CDS             6441..7247
                     /gene="proC"
                     /locus_tag="IVW65_001118"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7900033.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7326..8882)
                     /gene="rny"
                     /locus_tag="IVW65_001119"
     CDS             complement(7326..8882)
                     /gene="rny"
                     /locus_tag="IVW65_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7900034.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9293..10342)
                     /gene="recA"
                     /locus_tag="IVW65_001120"
     CDS             complement(9293..10342)
                     /gene="recA"
                     /locus_tag="IVW65_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7900035.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10451..11692)
                     /locus_tag="IVW65_001121"
     CDS             complement(10451..11692)
                     /locus_tag="IVW65_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7900036.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11932..12510)
                     /gene="pgsA"
                     /locus_tag="IVW65_001122"
     CDS             complement(11932..12510)
                     /gene="pgsA"
                     /locus_tag="IVW65_001122"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7900037.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12719..13603)
                     /locus_tag="IVW65_001123"
     CDS             complement(12719..13603)
                     /locus_tag="IVW65_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7900038.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(14381..15667)
                     /locus_tag="IVW65_001124"
     CDS             complement(14381..15667)
                     /locus_tag="IVW65_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7900039.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15660..16928)
                     /locus_tag="IVW65_001125"
     CDS             complement(15660..16928)
                     /locus_tag="IVW65_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7900040.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17180..17641
                     /gene="mscL"
                     /locus_tag="IVW65_001126"
     CDS             17180..17641
                     /gene="mscL"
                     /locus_tag="IVW65_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7900041.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17700..18146)
                     /locus_tag="IVW65_001127"
     CDS             complement(17700..18146)
                     /locus_tag="IVW65_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7900042.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18239..18484)
                     /locus_tag="IVW65_001128"
     CDS             complement(18239..18484)
                     /locus_tag="IVW65_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900043.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18599..19177)
                     /locus_tag="IVW65_001129"
     CDS             complement(18599..19177)
                     /locus_tag="IVW65_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7900044.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19254..19784)
                     /locus_tag="IVW65_001130"
     CDS             complement(19254..19784)
                     /locus_tag="IVW65_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7900045.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19788..20648)
                     /gene="rlmB"
                     /locus_tag="IVW65_001131"
     CDS             complement(19788..20648)
                     /gene="rlmB"
                     /locus_tag="IVW65_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7900046.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20641..21036)
                     /locus_tag="IVW65_001132"
     CDS             complement(20641..21036)
                     /locus_tag="IVW65_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7900047.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21033..22445)
                     /locus_tag="IVW65_001133"
     CDS             complement(21033..22445)
                     /locus_tag="IVW65_001133"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7900048.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22442..22978)
                     /gene="cysE"
                     /locus_tag="IVW65_001134"
     CDS             complement(22442..22978)
                     /gene="cysE"
                     /locus_tag="IVW65_001134"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7900049.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(23300..24760)
                     /locus_tag="IVW65_001135"
     CDS             complement(23300..24760)
                     /locus_tag="IVW65_001135"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7900050.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24930..26087)
                     /locus_tag="IVW65_001136"
     CDS             complement(24930..26087)
                     /locus_tag="IVW65_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7900051.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26121..27491)
                     /gene="radA"
                     /locus_tag="IVW65_001137"
     CDS             complement(26121..27491)
                     /gene="radA"
                     /locus_tag="IVW65_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7900052.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27554..28081)
                     /locus_tag="IVW65_001138"
     CDS             complement(27554..28081)
                     /locus_tag="IVW65_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7900053.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         7705 a         6195 c         4967 g         9224 t
ORIGIN      
        1 aaatttttcg tttttataag gcttcttttc tttataattt agttattttt tcttctgaag
       61 gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa gactaatgtg
      121 attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga cagcatccct
      181 agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc taatcgcata
      241 ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc aatcgggatc
      301 atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa atgaacgggt
      361 agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg ccgtttgaat
      421 aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac ttgctcccat
      481 gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga attccctccg
      541 ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat ggcaatggag
      601 atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc tgctaataca
      661 acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa cgctacccct
      721 gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat cactaaaaac
      781 acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc tcttctcatg
      841 aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg atctgtctgt
      901 ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat ttcccatgga
      961 catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca tccatacctg
     1021 ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc gctaaactga
     1081 ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata tcccacatcc
     1141 ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga cgcttgaacc
     1201 agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta ggaaaacctg
     1261 cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt ccttcttgat
     1321 ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac gatgctttca
     1381 ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca cccgcatcta
     1441 catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc aaatcagata
     1501 tttcgatata acgcacctta caacctccat tctaaaactt aattgatact ttcttttaaa
     1561 gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat ttgttcatta
     1621 gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt cgacaatgtc
     1681 tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt gtttttcttc
     1741 acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg tgaaacgccc
     1801 gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc agcatgctga
     1861 gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg atccagtgct
     1921 tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt atctggatat
     1981 ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac cggatcgagc
     2041 caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc ttcatgttcg
     2101 tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat caaggtctgt
     2161 ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga actattatac
     2221 acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc gaaatcatgc
     2281 ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac gatctctttt
     2341 gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt tttgtctgct
     2401 tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac cgtagctgtc
     2461 aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt ttacgattta
     2521 ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc atcaatttct
     2581 gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg ctcatatcta
     2641 gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca gtttttcatc
     2701 acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt cctcacaaaa
     2761 accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca aggcaaaaac
     2821 ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca tacgctcttt
     2881 gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt ttcccatttt
     2941 tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt ggatgtcttg
     3001 atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga cactcattct
     3061 tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga tttcttcccc
     3121 tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg taccgacttc
     3181 caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt ggttgatccc
     3241 tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta ctggaatatt
     3301 cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta gcaataaagc
     3361 agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg tttctacaat
     3421 gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta cgctcgacat
     3481 caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca gtcctaaatt
     3541 tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa aagtctaagt
     3601 cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct agtggttgat
     3661 tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa caagttctag
     3721 tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc ctttttcttt
     3781 tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt tcctataagc
     3841 aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag cagactggaa
     3901 ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt gaaagaaaga
     3961 aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt ccgagtagcc
     4021 cattttctcg tatacaataa acagattggc taacagatag tcagctaatt cctcagattc
     4081 tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg gctgagcggt
     4141 gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag ataatacata
     4201 tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa gccatttttg
     4261 tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc aagtaaaatg
     4321 gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag acaagtcttc
     4381 tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac ccaattcatg
     4441 aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc ctaaacaatc
     4501 tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc caactggatc
     4561 tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt gatcttggtc
     4621 gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc ttgtagctgt
     4681 gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac tgccaagctt
     4741 cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg agatgcccaa
     4801 accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat gatttcctgt
     4861 tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa cgataaacaa
     4921 aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga attttgttcg
     4981 ataaaattct tttctggata accacacagc taacagaatc aagacatata ccaatatcaa
     5041 ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta aagcaatcgt
     5101 aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga gtaataaaaa
     5161 gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca ataatacttg
     5221 aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca tcatgatccc
     5281 cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa acagtcgcgg
     5341 aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca atagaagtcc
     5401 cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa ctactgctac
     5461 atcttgccga acagacaatt gactcattcc taagatcatc aaaacgtcaa aagtccccat
     5521 tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca taggatagat
     5581 cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata ccgcaagggc
     5641 tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg gataaaactc
     5701 gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg ctaaaaaact
     5761 accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa taaaaaagac
     5821 atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata gtgcgacttt
     5881 tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac tagcccgcag
     5941 acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg ttacccacgc
     6001 agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta agactctgat
     6061 cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca gaatcatgag
     6121 caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc aactcatgcc
     6181 attagcaata tttgcgactt gatttgccac aaagactaac acggagccga aaaaaatcag
     6241 ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt tatttttcac
     6301 attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc ttatttattt
     6361 taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa agatatccta
     6421 atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat atggcacaag
     6481 ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg ttcgtttcca
     6541 gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat gcatgcaaaa
     6601 gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa ccaaaaatca
     6661 ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc atttctattg
     6721 cttcaggaaa atcattaata gatttagaaa cagcgcttga agatcctcag gcctccatcg
     6781 tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg atttgcaaaa
     6841 atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa gcaatcggca
     6901 gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct ggcagctcac
     6961 ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg aatggtttgt
     7021 ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt caaaaattcc
     7081 tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct ggcggtacaa
     7141 ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc atcaaaggaa
     7201 tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga tagcaatgtt
     7261 ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt cttttgtatt
     7321 ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac tttgatgtgt
     7381 ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa acgaacagcg
     7441 tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc agcttgaact
     7501 gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact tcttaaacga
     7561 cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga gagcgcatct
     7621 gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg atgagaagcg
     7681 atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc accgatctca
     7741 acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag caacccggca
     7801 cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc taattttgca
     7861 acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg cagacgtccg
     7921 atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc tgcctcttcc
     7981 ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat ttcttcgata
     8041 cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg tgcgatttct
     8101 cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc gatgattaaa
     8161 tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc aatgatacga
     8221 cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga aacttgatct
     8281 gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc tgcttcttct
     8341 ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag ctcttcttca
     8401 gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat tttttctagt
     8461 tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc aattaattgc
     8521 tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt ttctaacgag
     8581 tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg agattttaat
     8641 tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg attttcttcc
     8701 ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc actatcgatg
     8761 attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga ctttgcgacc
     8821 ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat gttgaatacc
     8881 ataaaatcca cctccatact atctttttca tttttatcat ctatacagct tctatgataa
     8941 attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa catgcacaac
     9001 tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa acggctttct
     9061 acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat aggtaaacct
     9121 ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata tttttatttt
     9181 atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa aaaacggcag
     9241 gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat ttttattctt
     9301 ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact tcttcaccaa
     9361 taccataagc tgctctaact agagcagaaa cttctgccat catttcagga tgattagcca
     9421 tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa gaataccaag
     9481 caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct ccttcttgtg
     9541 agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt gctactttgt
     9601 tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt ttcaattgtt
     9661 ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt cctccaggag
     9721 tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg atgaaaatag
     9781 cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga gacatcaaac
     9841 gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct cgtggaacta
     9901 aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact aaagcatcag
     9961 cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca tcgatattca
    10021 cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg aaagcggccg
    10081 ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt ttacctgaac
    10141 tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca cctaaagcga
    10201 catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt ttttcgccca
    10261 atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca gcatctaagg
    10321 ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat cagagcaaca
    10381 tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac cgaacaaata
    10441 ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct tgcataaccg
    10501 cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa accacgccgt
    10561 ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac gtgtccggtc
    10621 cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt gcttgaatgg
    10681 ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc tccggatcaa
    10741 tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct ggaaatactt
    10801 cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc aaactttccg
    10861 ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt gctagcgaat
    10921 tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt tcttctaatg
    10981 ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt cgtaatgtca
    11041 cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca atcagatctt
    11101 ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact ttagaaatta
    11161 gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc acaaacattg
    11221 gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct gtttgataaa
    11281 aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg ataatctggg
    11341 actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat gccaataatt
    11401 ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca gttgcttctt
    11461 ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt tcacttcttg
    11521 tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca actgtttgat
    11581 aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca ttcgtattca
    11641 cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc attacttccc
    11701 gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag agggcaagga
    11761 gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc tcctgccaaa
    11821 acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg aaattatccc
    11881 ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata cttacattga
    11941 tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga tagtaaagat
    12001 cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga atgggatatt
    12061 attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt ttcccggcca
    12121 agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg tgacggctaa
    12181 ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt caaccaacat
    12241 gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact tgccaaaatt
    12301 agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga tactagctaa
    12361 agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc ctacgttaaa
    12421 ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca tacaaattct
    12481 aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc ctattctacg
    12541 tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt tattcaaagg
    12601 agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga cacagatttg
    12661 tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat ttatacgatc
    12721 attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt tcccctggat
    12781 taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca ccaatcgtga
    12841 tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt gctgccagtg
    12901 tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga ttgacagcag
    12961 taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg gcactgccag
    13021 aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt gttgattcta
    13081 ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc tcgactgatc
    13141 cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga cgatcttgcc
    13201 aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat aaaatgacag
    13261 gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct tttctagaag
    13321 tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg cttttcccat
    13381 caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat tgccgaataa
    13441 atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg attgcttcca
    13501 aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt tttttattca
    13561 ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca tcttctccag
    13621 tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt tgggtgctgt
    13681 gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct gccaactcct
    13741 cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact tgttccttgt
    13801 gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca atcagctgtt
    13861 cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt atgtttctct
    13921 aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt caaaagcttg
    13981 catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt tacttggctg
    14041 ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg tgttccagaa
    14101 gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat tttcgtaaat
    14161 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta cttcgtttga
    14221 ttttatttta atataagttg attaaaagag agcatgattt gttttatttt ctcactaatt
    14281 tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc tactacaagt
    14341 ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt ctcatgtaaa
    14401 actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg tctttcattt
    14461 ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat aaactttgcg
    14521 taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt ccaagcattt
    14581 tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg aatccaagaa
    14641 tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg ctgaagtccg
    14701 caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg ataattcctt
    14761 gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg aacaacagat
    14821 tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg tctaatcctt
    14881 taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt tgtttgatga
    14941 tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg aaattttttg
    15001 cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta ccaactacaa
    15061 ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag tcttgagctg
    15121 tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg tgtgtaggat
    15181 acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca tacatttgga
    15241 tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag taaggtgctt
    15301 gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta gaaaacagat
    15361 agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc ccaaataatt
    15421 gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg gcaattccat
    15481 ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg atcgaaccat
    15541 aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac aaatacacgg
    15601 tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca tattctgttt
    15661 tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat ttgctcagct
    15721 acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc ttcatcagat
    15781 aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc ttgcggagag
    15841 tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag ttccaactct
    15901 gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag tacttgattt
    15961 cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt atccacgcta
    16021 ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag tttagagtga
    16081 ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg ttcatcgtaa
    16141 tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc acgtacttgt
    16201 cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata aactgtttcc
    16261 cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc aacatcgccg
    16321 acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga cgctagcgaa
    16381 ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt ttgttccggg
    16441 ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt atcttccttc
    16501 aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa ctgatcaaac
    16561 tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac cgcttgagaa
    16621 aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc gtttagccaa
    16681 tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag ctctgcaagt
    16741 tgcgcactta actttgtctg atccgaataa ttcaaactat ttgtttccaa catgctgctg
    16801 agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg gataaaaatc
    16861 cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc tgccaattga
    16921 tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt ttaatgggtc
    16981 aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca attcgccgtt
    17041 ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca aaagaaacag
    17101 acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta aagacaaata
    17161 aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc gtggagatgt
    17221 cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg taaaccaagt
    17281 ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca caggtacttc
    17341 tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg tcacatttgc
    17401 gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg tcttattctt
    17461 agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc aagtggaagt
    17521 ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc ttgccaaaca
    17581 agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata cttctaaata
    17641 ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt tttagattct
    17701 taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc caaatacaca
    17761 tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc ttgtgcgccg
    17821 atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc ttgaacaatc
    17881 tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc aaaatggata
    17941 ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa
    18001 aacgtatgcc aagcttctcc atcaatgtca tcaacttctt gataaggaca ctgttgttcc
    18061 acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt tgacaattct
    18121 tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt attcttagca
    18181 taccgattcg cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg tttgcttttt
    18241 aaactgcttc agttaaaaat tcgatctcaa ctgtccgagt aagtacatct gaatagctgt
    18301 aagatactcg ttcaaaagaa ttttcttcag gatccaaatc aacaacgaat actgaaggat
    18361 aggtttctgt taaaacgccg cggcgttctg tttgacgctt tcttcctgtc tgagcaacca
    18421 acatgattgg gctgccgata cgaccttcta aatctttctt gattgaagct aaagttgttg
    18481 gcatacagtt cacctctact atcaactata ccacatattt ttaaaatttt caagtttacc
    18541 ataaaaaaaa aagaaatgca ataaaaataa ataagaagct cattaagatt tttctaaatt
    18601 attcctcatt ataaaatttc aagtgttttt ttattttgag ttttactctc ccgtaagcgc
    18661 tcattacttg cttgtgatcc attgctaaac attcagccat gtctacaaaa tcaacgcctt
    18721 gcatataata atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg caattttcga
    18781 ttgcttcttt taataaaaga tgctgaattg ctaaaggttc ttgcaatgtg atctctgcta
    18841 aaaagtctgg tccttctgtc tctatttttt ggtcgattga gacttcatct ttttgatcac
    18901 gacgcttcag cgcgttctgc ttcctcaaca gagaatagat ttgattgctt ataacgcttt
    18961 tataataact tccaaagcta acacctttgc ttttttcgta agttctcaat gcctgatata
    19021 aagcgatcct tgcttcttgt tcccaatcct cagaaacata atttcttaaa taataggtgt
    19081 ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat gcttcttcgt
    19141 caccattacg tgcagcgcgc aataactcct cagacataaa ataattcctc cataaattcg
    19201 tttatggagg aatcttatca tcaatgattc atcgaactga ttagaacgat atgttattta
    19261 ttttttctca ttaaatcgtc caatttttgg gacaattttt ttaactgctc ttcattccat
    19321 ggagaattac gtcggaaatc aataaaatgc atatctttat tttcttgggc tattgttttt
    19381 tccgtttttt ttatcgtctt atataattca taagcagaag ttcgtaatgc tccttgggaa
    19441 aagatcatcc actgttctgc taaatcgctt gtcgccactg tcacttgtgt caaacggtca
    19501 ttcagctgac cagcgattct ctcgatataa ctgtcagccg tttcgtcttc tttcgtaaaa
    19561 acaacatcca actggtattt cttataattt tgttggatgc ccggaactag ttgggcatcg
    19621 aagacaacga tgacttccat tccttcatat ttcgcataat ttgataaacg atgtaaaaga
    19681 gcttctcttg catccgccat tttgtcctgc tttttaagct ggacaagttc cggccacgaa
    19741 ccaatcatgt tgtagccgtc tacaatcaac agctgttttt tcatggtcta ctccccctga
    19801 cgttgggaaa aagcctgata cataagcaaa ccagcagcaa cactggcatt taagctctgg
    19861 acgtgcccgg tcatcgggat cgtcagcaat tcatccattt cttttttcaa tcctggactc
    19921 attcctttgc cttcattacc aatgactaaa gcaattgatc ctttggcatt ccatttttgg
    19981 aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt gagctgttgc
    20041 acagcttgta ccaaattcgt cacgcgtgca acagggacat gttcgactgc cccagtggaa
    20101 gtctttgtta cgataggtgt gattccaact gctcggtgtt ttggtatgat cacaccatcg
    20161 acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc ttcgatgcta
    20221 tccaaaatca aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat cagttggtca
    20281 agggataaat attcataagg tgtaatcgca agaacgactc cttgatgcac gccgtgatcg
    20341 ctcaaagttt ctaatttttg tttaggaacc catttgactg gcacagcttg ttcacgtgcg
    20401 atttctttca gttcttctac tttttctcct cgtgaatctt cttgaagaaa gagtttattc
    20461 cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa attatcttct
    20521 aatgcttctt cattttgatc agaaactgac tgatttcgtc cagtttgctt tttatttgaa
    20581 tatctatttt gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt tgttctttct
    20641 ttacccattt ttttcaccaa ctttctgaat acaccagcgg atcaattctt ccatccgctc
    20701 ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg aaccctgttg cgacccgata
    20761 cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta gcatttcttc ctctgcgata
    20821 ataaagttct tcttcttctg tcaaaagatg ttcttccagc atttgctgaa tcaaaaaggc
    20881 ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc agcttattgg gttttgtttc
    20941 tccttgatca atcaggtggt cacgaacata gatttcgtaa atcgcatctc caacataagc
    21001 caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca tcgggttcct
    21061 tgcggtgtat cttctaataa gatccctttt tctttcaaca aatcacgaat ttcgtcgctt
    21121 cggacaaaat cacgattgct tcttgcttga tttcgttctt cgatcaattg ttcaatctct
    21181 tcatctaata actcttctgc tgtaaataaa ataccgaaga tcgaaagcca ttgcgagaat
    21241 aaagcttctc cttgtgccaa gacagcggca gaaactttcg gctggtctag ataagtgttc
    21301 agccacttag caagctcata gaccactgtg atcccatttg ctgcattaaa atcatcgtcc
    21361 atttcttcga tgaatcgctg ttctaaattt tctaacaaag cgattttatc cgcatcatct
    21421 gccaaattct ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt attcttcagt
    21481 cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac tgtagcggat cggacgacgg
    21541 tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat caacgttttt gatcatttca
    21601 tgtacagtga tgaaattccc tagagattta ctcatttttt catcgtcttc gccaatcgtt
    21661 acatagccat tgtgcatcca gtaattagcg aattttttcc ctgttttcgc ttcactttgt
    21721 gcgatttcat tttcatgatg aggaaattct aaatcttgcc cgccaccatg gatatcgatc
    21781 gtttctccta aatgttttgt tgccataacg gaacattcaa tatgccaacc gggacgtcct
    21841 ttgccccatg gtgaatccca agaaatctcg ccaggttttg cttgtttcca taaagcgaaa
    21901 tctaatgggt cttctttttg ctgctgttct tcccctgtac gctgacttgc tccgacttcc
    21961 agttcatcga tggattggtc acttaatttc ccataatttt caaatttacg ggtacggtaa
    22021 tacacatcac ctgaagattc ataagcatag cctttttcaa ccaacacttg aataaatgct
    22081 aaaatatctg gcatatgatc catgactctt ggatgcagtg tagcaggttc gacattcaac
    22141 gctttcgtat cttcttcaaa tgctcggatg aaacgatcag caacttctgg tgctgtaata
    22201 cctaattcgt ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt cgatacgaaa
    22261 tcgactttat agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc aatggtgcta
    22321 cgagcatttc cgatatggat ataattatac acagtcggtc cgcagacata cataccgaca
    22381 tgtccctttt gtatagggat gaattcttct ttttttctgg tcaatgtatt gtagattttt
    22441 atcattctga tctcctcact ttttctcctt tgattcgaac gacttttgct ggtactccta
    22501 ccgctgtagc gtcgtctgga acatctttca acacaacggc acctgcaccg attttggcgt
    22561 tttttccgat cgttaccggt cctaatatct gcgcattcgc agagagcatg gctccttgtt
    22621 taacagttgg gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt acgccatgga
    22681 ataaaatcac atcgtcctcg atttctgcgg tttcgccaat gacgattccc attccatggt
    22741 cgatgaacac gccagttcca atcgttgctc ctggatggat ctcaacacca gttaggaaac
    22801 gccagaattg agcattgatc ttcgccaata aaaagagccg gtgctgatat aaaaaatgag
    22861 agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc tcagctgtcg
    22921 aacgtgcagc cggatcgttt ctctttgctg catcaatcgc tcgggtaaac caactcatga
    22981 ctttccactc ctttcgtaag aattttatgt aggaaatcat cttcttataa agaaaagaag
    23041 cgccttttaa ataagtttcc ttatcaaaag acgctttccg cgtggttcca cttttcttca
    23101 taatcaatga ttatctctga cagctaacgc ctgttatacg ttcttggcta ctttgtttcc
    23161 tataaaacag tttttcctgt tttctgtgtt tcgccaagac cactcccaga ggcatttcgg
    23221 caaccattca ttgatacctt gcaccacccg gtatctctct tgtaatgatc agctgcttac
    23281 ttttttctgt tcatcgtgtt tagttcagta cttgatttaa atgtgctaat gatttttcac
    23341 gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct gatacagcta
    23401 cacggattgg catgaaaaga ttttttcctt ttacgccagt ttctttttga acagctttga
    23461 tggcagcttt gacagttgga gcatctacta cttccatttg ttctagctgt gctttgaatg
    23521 cgtggagaac agtaggaact gtttcaccag caagtacttc ttttgctgct tcattcaata
    23581 caggatgttc attgaaaaat agcgtagaca gatcaacgat ttctgcagca tagctcattt
    23641 gaggttgata caggctaacg atcttcttca cccattcgat tgtcttttca tctgggtttt
    23701 ctgctacacg tccatcagcg attagataag gcagacacat gtcagtaaat gcatctagat
    23761 ccattgcttt gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg aaagctgcgg
    23821 gtgatttact taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt tcgaagattt
    23881 cgtcttcgcc cacaggtgac cagcccaata atgcaataaa gttgaacatt gcatctggta
    23941 gatagcctaa ttcgcgatat tgttcgataa attgcaagat tgattcgtca cgtttgctta
    24001 gttttttacc tgtttcactg ttgatgatca atgtcatatg tccgaaggtt ggtggtgtcc
    24061 agccaaatgc ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg tcatctccac
    24121 gtaaaacgtg agtgattttc atcaaatgat catcgaccgc aacagcaaaa ttataagttg
    24181 gcataccatc gcgtttttgg atgacaaagt cgccaccgat attatcggat tcgaacgaaa
    24241 tagcgccttt caccatatct gtaaatgcgt attctgtatt gcgaggcaca cggaaacgta
    24301 caacagattc tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc aagttcgcac
    24361 atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt tctgcttcta
    24421 attcttcttc tgtgcaatag catttgtatg ctcgattgct agcaagcagc tgatcaatca
    24481 gcggttgata gatttcttta cgttcagatt gacggtaagg gccgtattct ccaggatttt
    24541 caggagattc atcccaattc attcccaacc aagcaaggtt ttccaactgg cttttttcgc
    24601 catcttcgat attacgtttt tggtcagtat cctcaatacg aataataaag tctccatcat
    24661 tgtggcgggc aaacaaataa ttgaataatg cagttcttgc atttccaata tgcaggtgcc
    24721 cagttggact tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc ttctttcaaa
    24781 ctagactttg actaacagca aaaattccgt cagttacggc ttctcaaata tcatttattt
    24841 aaagccactc agcacaaatt ttaccttttc taaggtaaat agtaaagcca actggttgaa
    24901 tttccttacc agttggctga atttcttttt tattttgatt gtttagtctt tttctctgtc
    24961 tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat catgcgtcca
    25021 gcagctgttt gaagggcact tgttacaacg acttcgatac gttcattcat gtaatgctgt
    25081 ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg ctactccttg ttggcgttct
    25141 gtcccggctt ttactactag aacattcatc gtttcaccag ggatgacgac tggttttacg
    25201 gcatttgcca acgcattgat attaagaaca ggtacatttt ggaattcaga gactttattc
    25261 aagttatagt cattggttac aatgacacca tctaaaagct tagccaattt gatcaatttg
    25321 ctgtctactt cactgatatc ttcaaagtct ccatcgtaca tttcgacaga tataccctct
    25381 tctttttgca aggcatttag gatatctaat ccgcgacgac cacgcacacg tttcaaacta
    25441 tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa aattagggat catcaaggtt
    25501 ccttctaaga atccagtctt tgtaatatca taaatccgac cgtcaatgat cacgcttgta
    25561 tccaagattt tatatttatg gaaattttct cctgattttc gttctaacag ttgactctca
    25621 acttgactag ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac tttcttccat
    25681 tcttcgattc gtgtcgttcc cattctcaat cccagatagc ctaagatgat catcagtaaa
    25741 atcggcaata cactattgac gaatggaatt tccatattgt aaagaggggt cgagatcaca
    25801 acacctaaag tcaatccgat gatcgcaccg atcgcaccga agagaagata agtcaagctg
    25861 acctcactta gtttttgttc tacttgtttg ataatagctg agatatgttt ggccaacagc
    25921 aaagataata aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt attattcagc
    25981 catccgtttg actgttgacc gattgcctgc caagctgagg gtaaaaatga aatacccaag
    26041 cttgcgccga caatgatcat cagcaaagta atgacacgtt tttgcatcgt ttttcctccg
    26101 tttcgacaat catttattag ttagcggaac acacgcttca acgtttcagc caaagttgct
    26161 actccaacta tctcgattcc ttttggcgct tcccagttac ctaagttgtt tttaggtagg
    26221 tatacttttg tgaagccgag tttttggact tctttgactc gttgttcaat atgactgaca
    26281 cgtctgattt ctccagtcag tccaatttca ccgatgaaac attccgttgg ctgtgttcct
    26341 ttttctttat agcttgaagc aatactgaca gcgatagcta aatcaatcgc tggttcattt
    26401 aattttaccc cacctgcagc ttttaagtaa gcatcttgat tctgaagaag caacccagct
    26461 cttttttcca gcactgccat gatcagagat acccgattga aatcaagacc tgttgttgtt
    26521 cgtttggcat ttccgaacat agtaggtgtg actaatgctt ggatctctac caaaattgga
    26581 cgtgtccctt ccattgccac aacgatagca gagccagttg caccgtctag gcgttcttct
    26641 aagaaaatct gtgaaggatt agcgacttct tccaatccat gctcatgcat ttcaaaaatc
    26701 ccaatctcat tcgttgagcc aaatcggttt tttactgctc gcaaaatccg aaaactatga
    26761 tgttgttcac cttcaaaata taacaccgta tccaccatat gttctaacat tcttgggccg
    26821 gcgatcgagc cttctttggt aacatgaccg acgataaaaa tggctatccc atttgtttta
    26881 gcggttttga gtagttctgc agtggtttct cttacttggc tgacacttcc tgccacactt
    26941 gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga taacataatc aggggagata
    27001 tgttcgatgg cacgactgat ttcgttcata tctgtttcag cgtatagata aaattctgtg
    27061 ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc ttcgccggat
    27121 acatataaga cttttccgcc tatggctgct aattgctgag aaacttgtaa cagcaaagtg
    27181 gatttcccga tacctggatc gccaccaatc aagaccatag accctggcac cacacctccg
    27241 cctaacacac ggttcagttc ttctaatttt gttttgatcc gaggttcttt tttagggacg
    27301 acgtctgcga tttttgttgg ttttgctttt tctcctgtca aacttgtccg agttctgcgg
    27361 tccgttgtat cttgttcgat ttcttcgacc atcgtattcc attttccgca atttgggcag
    27421 cgtcccaaga acttaggcga cacatacccg caattttggc aaacaaattg tacttttgat
    27481 ttttttgcca ttgtaccgcc tccttttatc ctaatcatga gacattaaat caagcttaag
    27541 tatctttaac gttttattta ctggaagaac caaatccgcc ttgacgttga gcagtcgttt
    27601 gatcttgatc tgcttttaaa aacggcaaaa agatcccttg gccgatacgc tcgccttttt
    27661 tgatcactac atccttgaaa ccaaagttgg tgaattggaa catgatatgg ccttcatttg
    27721 cctcattatt gtaataatca ctgtcaatca cacctactcc gtttgtcaac actaaaaacc
    27781 gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct cccatgtaag
    27841 ctttgattcc agtaggcacg agggttggtt tcaattgttt ttcattttct gatacatctt
    27901 ttttcaatcc atctgcgact gtttccaacc cctttttcca gcctgctttc cagatacttg
    27961 gaatcacgac ctcttctgct gcttcaaaat catagccagc agcatgatgt gtagcgcgct
    28021 gcggtagatt gatattttga tctttatatt tagaaataac ttcaaatcct ctttttctca
    28081 ccggttttcc t
//
LOCUS       DACCWK010000028        26832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCWK010000028 DACCWK010000000
VERSION     DACCWK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.028"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..833)
                     /locus_tag="IVW65_001139"
     CDS             complement(<1..833)
                     /locus_tag="IVW65_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7900054.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     Y"
     gene            complement(1057..2997)
                     /gene="thrS"
                     /locus_tag="IVW65_001140"
     CDS             complement(1057..2997)
                     /gene="thrS"
                     /locus_tag="IVW65_001140"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7900055.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3334..3513)
                     /locus_tag="IVW65_001141"
     CDS             complement(3334..3513)
                     /locus_tag="IVW65_001141"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7900056.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3560..5383)
                     /gene="cadA"
                     /locus_tag="IVW65_001142"
     CDS             complement(3560..5383)
                     /gene="cadA"
                     /locus_tag="IVW65_001142"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7900057.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5661..7940
                     /locus_tag="IVW65_001143"
     CDS             5661..7940
                     /locus_tag="IVW65_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7900058.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8033..8680)
                     /locus_tag="IVW65_001144"
     CDS             complement(8033..8680)
                     /locus_tag="IVW65_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900059.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8859..9578)
                     /locus_tag="IVW65_001145"
     CDS             complement(8859..9578)
                     /locus_tag="IVW65_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7900060.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9709..10860)
                     /locus_tag="IVW65_001146"
     CDS             complement(9709..10860)
                     /locus_tag="IVW65_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7900061.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11077..11817)
                     /locus_tag="IVW65_001147"
     CDS             complement(11077..11817)
                     /locus_tag="IVW65_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7900062.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11821..12144)
                     /gene="rsfS"
                     /locus_tag="IVW65_001148"
     CDS             complement(11821..12144)
                     /gene="rsfS"
                     /locus_tag="IVW65_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7900063.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12201..12794)
                     /locus_tag="IVW65_001149"
     CDS             complement(12201..12794)
                     /locus_tag="IVW65_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7900064.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12778..13425)
                     /locus_tag="IVW65_001150"
     CDS             complement(12778..13425)
                     /locus_tag="IVW65_001150"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7900065.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13438..13749)
                     /gene="yhbY"
                     /locus_tag="IVW65_001151"
     CDS             complement(13438..13749)
                     /gene="yhbY"
                     /locus_tag="IVW65_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7900066.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13765..14874)
                     /gene="yqeH"
                     /locus_tag="IVW65_001152"
     CDS             complement(13765..14874)
                     /gene="yqeH"
                     /locus_tag="IVW65_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7900067.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14871..15398)
                     /locus_tag="IVW65_001153"
     CDS             complement(14871..15398)
                     /locus_tag="IVW65_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7900068.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15578..16363)
                     /locus_tag="IVW65_001154"
     CDS             complement(15578..16363)
                     /locus_tag="IVW65_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7900069.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16356..17225)
                     /locus_tag="IVW65_001155"
     CDS             complement(16356..17225)
                     /locus_tag="IVW65_001155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7900070.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17226..18605)
                     /locus_tag="IVW65_001156"
     CDS             complement(17226..18605)
                     /locus_tag="IVW65_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7900071.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18613..19032)
                     /gene="fabZ"
                     /locus_tag="IVW65_001157"
     CDS             complement(18613..19032)
                     /gene="fabZ"
                     /locus_tag="IVW65_001157"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7900072.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19057..19533)
                     /gene="accB"
                     /locus_tag="IVW65_001158"
     CDS             complement(19057..19533)
                     /gene="accB"
                     /locus_tag="IVW65_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7900073.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19536..20771)
                     /gene="fabF"
                     /locus_tag="IVW65_001159"
     CDS             complement(19536..20771)
                     /gene="fabF"
                     /locus_tag="IVW65_001159"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7900074.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20790..21527)
                     /gene="fabG"
                     /locus_tag="IVW65_001160"
     CDS             complement(20790..21527)
                     /gene="fabG"
                     /locus_tag="IVW65_001160"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7900075.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21539..22456)
                     /gene="fabD"
                     /locus_tag="IVW65_001161"
     CDS             complement(21539..22456)
                     /gene="fabD"
                     /locus_tag="IVW65_001161"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7900076.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22563..22787)
                     /locus_tag="IVW65_001162"
     CDS             complement(22563..22787)
                     /locus_tag="IVW65_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7900077.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22840..23802)
                     /locus_tag="IVW65_001163"
     CDS             complement(22840..23802)
                     /locus_tag="IVW65_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7900078.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23812..24252)
                     /locus_tag="IVW65_001164"
     CDS             complement(23812..24252)
                     /locus_tag="IVW65_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7900079.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24558..25523
                     /locus_tag="IVW65_001165"
     CDS             24558..25523
                     /locus_tag="IVW65_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7900080.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25580..25810)
                     /locus_tag="IVW65_001166"
     CDS             complement(25580..25810)
                     /locus_tag="IVW65_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7900081.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25896..26783)
                     /locus_tag="IVW65_001167"
     CDS             complement(25896..26783)
                     /locus_tag="IVW65_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7900082.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
BASE COUNT         7415 a         5917 c         4638 g         8862 t
ORIGIN      
        1 aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta
       61 gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt
      121 gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc
      181 ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc
      241 gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc
      301 actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa
      361 atgattggct taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag
      421 gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac gactttttgg
      481 atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca
      541 agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg
      601 attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg
      661 atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca
      721 ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt
      781 tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct cataagttcc
      841 tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt
      901 atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc
      961 acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc
     1021 tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt
     1081 gacttctgct tccatagcat caataaagat attcaaatct tctacagctg tttctttact
     1141 tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg
     1201 gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc
     1261 atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc
     1321 gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg
     1381 aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat
     1441 tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa
     1501 atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc
     1561 aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc
     1621 ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt gggctttttc
     1681 ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg
     1741 gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg
     1801 tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat
     1861 ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc
     1921 taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat
     1981 catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat
     2041 tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac
     2101 atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat
     2161 ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga
     2221 acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct
     2281 ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc
     2341 tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc
     2401 cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca
     2461 caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact
     2521 aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag
     2581 aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc
     2641 cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga
     2701 atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc
     2761 catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat
     2821 gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac
     2881 tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt
     2941 tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat
     3001 agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc
     3061 tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa
     3121 cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc
     3181 gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta
     3241 tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac
     3301 taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc
     3361 gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt
     3421 gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg
     3481 accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc
     3541 ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg
     3601 ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca
     3661 aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac
     3721 aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca
     3781 ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac
     3841 tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg
     3901 gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt
     3961 ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt
     4021 tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca
     4081 catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa
     4141 ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt
     4201 ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta
     4261 ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt
     4321 ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag
     4381 ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt
     4441 ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga
     4501 gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc
     4561 ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta
     4621 cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag
     4681 caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt
     4741 cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt
     4801 cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag
     4861 gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc
     4921 ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat
     4981 caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa
     5041 ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat
     5101 cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt
     5161 tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg
     5221 aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg
     5281 cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc
     5341 caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt
     5401 tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt
     5461 ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg
     5521 tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc
     5581 atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg
     5641 gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa
     5701 cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc
     5761 aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg
     5821 aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat
     5881 accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc
     5941 cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta
     6001 tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg
     6061 ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa
     6121 tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta
     6181 tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat
     6241 caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc
     6301 gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg
     6361 ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa
     6421 acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt
     6481 cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc
     6541 cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag
     6601 atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc
     6661 aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc
     6721 tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc
     6781 atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg
     6841 aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa
     6901 cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca
     6961 accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg
     7021 tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac
     7081 gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc
     7141 gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat
     7201 atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg
     7261 caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg
     7321 gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt
     7381 tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa
     7441 tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa
     7501 aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag
     7561 cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta
     7621 aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag
     7681 tccagctgat tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct
     7741 ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt
     7801 tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat
     7861 tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt
     7921 atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct
     7981 ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc atttattcta
     8041 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
     8101 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
     8161 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
     8221 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
     8281 tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
     8341 caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
     8401 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
     8461 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
     8521 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
     8581 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
     8641 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
     8701 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
     8761 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
     8821 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
     8881 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
     8941 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
     9001 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     9061 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     9121 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     9181 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     9241 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     9301 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     9361 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     9421 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     9481 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     9541 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     9601 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     9661 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     9721 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     9781 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     9841 ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata
     9901 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
     9961 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
    10021 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
    10081 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
    10141 ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac
    10201 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
    10261 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
    10321 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
    10381 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
    10441 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
    10501 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
    10561 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
    10621 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
    10681 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa
    10741 attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac
    10801 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
    10861 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
    10921 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
    10981 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
    11041 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
    11101 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
    11161 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
    11221 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
    11281 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
    11341 attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga
    11401 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
    11461 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
    11521 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
    11581 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
    11641 tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag
    11701 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg
    11761 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
    11821 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
    11881 atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa
    11941 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
    12001 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
    12061 catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc
    12121 ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat
    12181 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
    12241 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
    12301 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
    12361 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
    12421 gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat
    12481 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
    12541 aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
    12601 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
    12661 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
    12721 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
    12781 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
    12841 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
    12901 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
    12961 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
    13021 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
    13081 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
    13141 ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc
    13201 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
    13261 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
    13321 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
    13381 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
    13441 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
    13501 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
    13561 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
    13621 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
    13681 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
    13741 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
    13801 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
    13861 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
    13921 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
    13981 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
    14041 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
    14101 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
    14161 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
    14221 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
    14281 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
    14341 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
    14401 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
    14461 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
    14521 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
    14581 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
    14641 gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
    14701 caatgagaca tcttggatct cattataatg gcgtaatctg aaacaacgct gacaatagac
    14761 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
    14821 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
    14881 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
    14941 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
    15001 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
    15061 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
    15121 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
    15181 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
    15241 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
    15301 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
    15361 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
    15421 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
    15481 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
    15541 aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt
    15601 atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca
    15661 acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt
    15721 caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca
    15781 actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg
    15841 gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag
    15901 caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat
    15961 cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta
    16021 caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg
    16081 cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc
    16141 tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc
    16201 catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat
    16261 ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt
    16321 tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc
    16381 tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc
    16441 tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc
    16501 tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct
    16561 gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc
    16621 gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt
    16681 tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg
    16741 aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct
    16801 ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag
    16861 ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct
    16921 tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga
    16981 tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca
    17041 ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta
    17101 taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag
    17161 ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga
    17221 gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt
    17281 aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga
    17341 aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc
    17401 attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa
    17461 tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct
    17521 gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt
    17581 tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca
    17641 ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc
    17701 atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg
    17761 ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct
    17821 gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga
    17881 gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga
    17941 atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg
    18001 attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa
    18061 gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca
    18121 cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct
    18181 tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc
    18241 aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata
    18301 aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca
    18361 aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca
    18421 ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt
    18481 gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg
    18541 cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa
    18601 aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc
    18661 tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc
    18721 aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc
    18781 gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg
    18841 agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg
    18901 aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat
    18961 ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc
    19021 ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag
    19081 tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc
    19141 ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc
    19201 tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata
    19261 aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg attctttttc
    19321 tttcattatt tctggagcag ttgttgcttg ttcagatatg acattgactg gctccgaaac
    19381 tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat
    19441 atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg
    19501 actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac
    19561 agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca
    19621 ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg
    19681 atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg
    19741 ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg
    19801 aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta
    19861 cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc
    19921 gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa
    19981 gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt
    20041 gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc
    20101 cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt
    20161 aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga
    20221 atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg
    20281 ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata
    20341 tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca
    20401 cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat
    20461 ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc
    20521 attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct
    20581 tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct
    20641 gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta
    20701 ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc
    20761 actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca
    20821 ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata
    20881 tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg
    20941 tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta
    21001 atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta
    21061 ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta
    21121 atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag
    21181 gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca
    21241 agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct
    21301 tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct
    21361 acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga
    21421 ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt
    21481 cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt
    21541 atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg
    21601 ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt
    21661 gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga
    21721 cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc
    21781 ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt
    21841 ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc
    21901 ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac
    21961 caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag
    22021 cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc
    22081 cgcttccagc tggggtagct tctgtcatat aagcgcctct ttttgctact aacgcaactg
    22141 cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc
    22201 ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga
    22261 ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc
    22321 aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa
    22381 cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata
    22441 agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat
    22501 tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat
    22561 gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct
    22621 tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc
    22681 aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct
    22741 tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat aatatattcc
    22801 tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact
    22861 tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt
    22921 gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt
    22981 cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat
    23041 tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt
    23101 catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc
    23161 agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg
    23221 ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag
    23281 caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac
    23341 agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag
    23401 tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc
    23461 tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac
    23521 ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc
    23581 caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa
    23641 atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat
    23701 ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg
    23761 aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata
    23821 gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt
    23881 agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa
    23941 tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg
    24001 gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac
    24061 agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc
    24121 ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga
    24181 ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa
    24241 atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca
    24301 aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag
    24361 ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat aatttctttt
    24421 attagaaaaa tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac
    24481 tatagaattc cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa
    24541 gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg
    24601 tctatcttat gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca
    24661 aaaatagagt aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta
    24721 tgattcatca tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat
    24781 tgctcgagtg tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt
    24841 tcagtaatta taattatgta catattacca ataagggatt tgtattaaca gatactccta
    24901 cggctcgaac atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa
    24961 tggaagaaac ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc
    25021 agcttcctga cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt
    25081 ttcaaggccg aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct
    25141 atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt
    25201 ttattagacg gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca
    25261 tgccattagc agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg
    25321 cagaaacaat gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag
    25381 ctcatttatc tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga
    25441 tcagttaccg caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag
    25501 aagctgaaaa gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa
    25561 atgtcaatta tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga
    25621 tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg
    25681 ctttatcata agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca
    25741 gttcattatc tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg
    25801 aatgtgtcat cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc
    25861 tacttgcatt gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt
    25921 ccttcaggcc accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct
    25981 gccaactctt catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt
    26041 tctgcttctt ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt
    26101 gtgtaatctt cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt
    26161 ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca
    26221 agcatttccg tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta
    26281 tgctgacctt tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa
    26341 ggcagtactt gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc
    26401 tttgctccta agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt
    26461 gctgtttttg caattttttc tgccaggctt ggtgtacttg tagtcaaatc aactaaaata
    26521 tgtcctgaga tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct
    26581 ttggggtaac ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta
    26641 tctgcccaga tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag
    26701 acagctactt cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct
    26761 gttccgataa aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct
    26821 atgctagtat ac
//
LOCUS       DACCWK010000029        26395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCWK010000029 DACCWK010000000
VERSION     DACCWK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.029"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(270..638)
                     /locus_tag="IVW65_001168"
     CDS             complement(270..638)
                     /locus_tag="IVW65_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7900083.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            788..1270
                     /locus_tag="IVW65_001169"
     CDS             788..1270
                     /locus_tag="IVW65_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7900084.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1307..2317
                     /locus_tag="IVW65_001170"
     CDS             1307..2317
                     /locus_tag="IVW65_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7900085.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2432..3145)
                     /locus_tag="IVW65_001171"
     CDS             complement(2432..3145)
                     /locus_tag="IVW65_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900086.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3335..3925)
                     /locus_tag="IVW65_001172"
     CDS             complement(3335..3925)
                     /locus_tag="IVW65_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7900087.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4409..4636
                     /locus_tag="IVW65_001173"
     CDS             4409..4636
                     /locus_tag="IVW65_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7900088.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4652..5818
                     /locus_tag="IVW65_001174"
     CDS             4652..5818
                     /locus_tag="IVW65_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7900089.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6052..7095
                     /gene="hrcA"
                     /locus_tag="IVW65_001175"
     CDS             6052..7095
                     /gene="hrcA"
                     /locus_tag="IVW65_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7900090.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7189..7752
                     /gene="grpE"
                     /locus_tag="IVW65_001176"
     CDS             7189..7752
                     /gene="grpE"
                     /locus_tag="IVW65_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7900091.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7803..9632
                     /gene="dnaK"
                     /locus_tag="IVW65_001177"
     CDS             7803..9632
                     /gene="dnaK"
                     /locus_tag="IVW65_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7900092.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9783..10949
                     /gene="dnaJ"
                     /locus_tag="IVW65_001178"
     CDS             9783..10949
                     /gene="dnaJ"
                     /locus_tag="IVW65_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7900093.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11031..11693)
                     /locus_tag="IVW65_001179"
     CDS             complement(11031..11693)
                     /locus_tag="IVW65_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP7900094.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEVHLFK
                     KTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLV
                     QT"
     gene            11669..11842
                     /locus_tag="IVW65_001180"
     CDS             11669..11842
                     /locus_tag="IVW65_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7900095.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11887..12624)
                     /locus_tag="IVW65_001181"
     CDS             complement(11887..12624)
                     /locus_tag="IVW65_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7900096.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12626..14128)
                     /locus_tag="IVW65_001182"
     CDS             complement(12626..14128)
                     /locus_tag="IVW65_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7900097.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14387..16381
                     /gene="uvrB"
                     /locus_tag="IVW65_001183"
     CDS             14387..16381
                     /gene="uvrB"
                     /locus_tag="IVW65_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7900098.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGSFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16391..19207
                     /gene="uvrA"
                     /locus_tag="IVW65_001184"
     CDS             16391..19207
                     /gene="uvrA"
                     /locus_tag="IVW65_001184"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7900099.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19470..20735
                     /locus_tag="IVW65_001185"
     CDS             19470..20735
                     /locus_tag="IVW65_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7900100.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20739..21470
                     /locus_tag="IVW65_001186"
     CDS             20739..21470
                     /locus_tag="IVW65_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7900101.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21605..22489
                     /gene="rapZ"
                     /locus_tag="IVW65_001187"
     CDS             21605..22489
                     /gene="rapZ"
                     /locus_tag="IVW65_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7900102.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22486..23484
                     /locus_tag="IVW65_001188"
     CDS             22486..23484
                     /locus_tag="IVW65_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7900103.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23518..24453
                     /gene="whiA"
                     /locus_tag="IVW65_001189"
     CDS             23518..24453
                     /gene="whiA"
                     /locus_tag="IVW65_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7900104.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24996..25646
                     /locus_tag="IVW65_001190"
     CDS             24996..25646
                     /locus_tag="IVW65_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900105.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25833..>26395
                     /locus_tag="IVW65_001191"
     CDS             25833..>26395
                     /locus_tag="IVW65_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP7900106.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8720 a         4450 c         5606 g         7619 t
ORIGIN      
        1 tatgaaagat cactgatatt ttctcgtatt ttttccttat tcaaaagttt tgcaggaaaa
       61 ttgctgaaaa aataatttta ctaagtagaa aattcaaagc cattcaatca gtgatttttt
      121 tcagcacaag aaagactcct tataaaaata gcagatactt tgtcaattaa gtgtttaacc
      181 agggatgata aaaaggatag gattgaacaa aacctgtata aggtgatgaa tagtttcctc
      241 gccagtaaaa ggtttattcg tataaagctt tatcgccaaa atttatcatg acctttttta
      301 tcttcaatat taaatgaaat caattgttca atcaattccc agttgattac acttttccaa
      361 ggaactttga tgacgttatc cgtatatacg tagcctgctt cttggatatt tttttcaagg
      421 gcacgaatcg ctgctttttc cggtgcgata gaaaaatgat ttttagcaaa actaaatgca
      481 ataataaagg taccatggtc agtaaacata ggttggttcc attttacagt tgtgtctagc
      541 tggggatacg tttctgttat cttagcaaaa aggtctttga tcttttgttg atgttctttt
      601 tctttgattt gattgatgat cggctgaaat ttttccattc taaaacgcct ccattaagtg
      661 gtttgattta ttttaccaga aaaacaatag ttatgtataa aaaacgaaca aaacacaagt
      721 aatgttgtaa ccgctatatt tatatgataa gatgtctcaa ataataaaat agaaaagaga
      781 cataaaaatg attgacttgg caaagaaatt cttacacgaa aatatatatt ttgagttgta
      841 tcatcatcga gccatttata caaatgaaga tgctttggta ataaagaaag aacaaggatt
      901 tacaggtaca gagaccaaaa gcttattctt aaaagataaa aaagataata actacgtata
      961 tctgactttt actacgaaac aaacggattt caaagcatta gggaagatcg tcggtaaaag
     1021 gctttctatt gtttcatcag aacgcatgga agaacaaacg ggacaaaagc agggcgctgt
     1081 ttctccgttt ggttatgaaa aacagattcc gattattatt gatgaagagc tcttatcaaa
     1141 tgaaaaactt gtttttgcac caggtagacc agatcaaacg atggtcatcc aaacggttga
     1201 cttaaacaaa atcatccagc tacttgagat gccgatattt ttgatgccgg caaacgaaaa
     1261 aaacaattaa ccaagtaaaa tagtgcatac ttggaggtga agcaacatga taagttatcc
     1321 attattagaa agaaatgcaa cgattggggt tactgcacca tcctcaggcg tatcagagga
     1381 acaccattcg ttactcgaat cagctttaaa gagaatggaa gaaaaatctt tccaagtagt
     1441 tactggaaag acgctttgga cgcaagataa ggaaaaatca ggtccagcaa gaaaacgagc
     1501 agatgaattc aatcaaatga tgcaagatga aaatatccaa atgatctttc cgacttgggg
     1561 aggagaatta ttgattgaga ttctcgattt tgtcgatttt gatgctctac aaccaaaatg
     1621 gatagtaggt tattcagata ttagtacgtt gctacttgct gtcacattaa aaacagggat
     1681 agccactgca catggaacta accttattga tttaagagga gagtacttgg atgaaacgac
     1741 agcaatgtgg gaaagtgtac tgaaaacaag agaaggagaa tcgattctac agcaatcttc
     1801 tgaaaagtat caaaaagtgt ggccccatga gcattcatca tcttctgttt tccatctcac
     1861 tgaaccaaca aattggcaat cagtcagcgg agatgcagta aggatgaagg gacgattatt
     1921 aggtggttgt attgatacga tcaggcattt aatcggtact ccttatggag atgtccaatc
     1981 ttttaaaaga aagtataatg atggaaagcc gatcttgtgg tttctagaaa actgtgagct
     2041 atcgtcagtt gatttaagaa gatcacttgt tcagatgaaa ctggcaggct ggtttgatgg
     2101 cagctcgggg atcatgttcg gtagaagtgc ggcagaggat ttgttagatt ataaaatgaa
     2161 ggatgtatac gaagatctgt caaaggaatt aaaactaccg atcttgtatg atattgattg
     2221 tggtcatgta ccgccacaac tgacattgat taatggtgcc tatgcagaag tagcggtaga
     2281 agatagaaaa gcgactgttt tgcaaacgtt tatttaaaaa attttttcct gccttggagt
     2341 ctatcaaaga ccaataaatc taaatagaaa agaaagtgaa agaagctgac aaaatgctgt
     2401 cagcttcttt ttgtataaca tattagaaag tctaattatc tataactgtc acatcgatta
     2461 actgtttcac gtcattggga tttcctgtag gaatagctac tacagatgca ttatcaatga
     2521 tttcttgagt tgtttctcta atgaacgctg gataagatgt ttgatctgca ggcatttgcc
     2581 aatgtgaagg aactggataa ctagtgattg tcccgtcatg gttacttgaa tacactaaaa
     2641 ctccctggta actgtatttc ccagtgagca taaccacatc tttcggatag actgcactcc
     2701 ctgcatcata aggggcgata ggtgttcctg caggataatg aataacatgt aattcgaaat
     2761 cacccgatgt atctctaaaa gtattacctc taatagctag ccaaacacgt gcgtactcaa
     2821 tttgtagatc agaatatcct gctaacggat ctattgactg agaagactgt gaggaggttg
     2881 tgtctattgt tgaagaagaa atatttgtct caactgtact actagatgta gaagaagtat
     2941 tgggctctga gactttactg cttgttgaag atgtattcgt ttcagtagaa gctgatttct
     3001 gagtacttga tttttcagct gaactggttt gtctgattga acttttgtct gtggtcgcag
     3061 atggatcact attttcttta ctaggagacg tacatcctac aaggaataaa gagaccaaca
     3121 ataaaataaa acctaacttt ttcatataaa atacctcatt atttttttaa taccagtata
     3181 gcataatcct gaatttttga atccgtaaaa ccttgtctat gaatataaat agagtgtatc
     3241 ttctttgata aatgtcagaa aaagtgtgac aaataatcat cagtaaaatt acttgtcaca
     3301 ctattgataa ggagtattta attgaagaga atgattagga gaaaggatct ccgtactttt
     3361 tatagtaatc atagaatcca agtttgtata gacgaatcgt tttttctctt atttcttctg
     3421 tttcaaaatg gactgcttga agaatcaagt ctgtgaattc tataggagct gttccattcg
     3481 cagtaatcaa attacgatct ttcacagctt gttgttctat aaaatcttct ttattctgat
     3541 aattttccga ttctaaccaa ggaaattgcg catttccggt gtgtttgtaa ccttgcaata
     3601 atccgctctg agctaaataa tcaactgccc cacaaatagc agctactggt acgtctgttt
     3661 ttaacaattt atcgattatt tgatataaat gatgattttt tatgtcccat gaattgccac
     3721 cgatcataat aaaaaggtca actttttcag gtatttgttc gattaagtaa tcgatttttg
     3781 tttttaatcc tccaatggaa gtgacctctg attggataga agcagttttg ttttgccatt
     3841 tctcattttg gtttagtgtg ctagtaatat aagcaccttc ccaatcggca tattcctcta
     3901 atagaaacgt aatcgcagtt ttcattttca agctctcctt tactatctat aacaaggaca
     3961 acagttttaa cctacagaca ttcattaaaa ttgggtagcg acagcgaaaa caacaattct
     4021 taagttttag acagagtata cagatagtca acttaattaa atacgaacta aataaaaatg
     4081 acaggtaatc atttttgttt accagcatta taacattttg catacatgga tgacaaaaga
     4141 accaattccc acttgcttga gtacaatatt aaaagaaact ggaaggcaaa aagtaattat
     4201 agaaacgaaa aataaatcat atcgggcact cagacatgaa aacaacccta gagatatata
     4261 actaggtaac cagtttaatg aaagaaaaaa tcatccaaga agtcgattct tggatttttt
     4321 agtggatttg ttcgccaaat acgtttagta tgcgtatatg agaggttgta agcaaagcgt
     4381 tgtggtctga gcacttaagg aggaatctgt gaaaatagct tttcatattt ttgcagattt
     4441 tgacttaatg ccgaaggtca gctttgcgat taccgtttat cgagaggctg taaaagcacc
     4501 gtgtagctcc gatgacaata agcaagcatt tttagagcta ggtttccttg attctaaata
     4561 tgcggcttat ttcaatgagt tggtgcttga acgcgatttt aagaaggtcg aaaacaggca
     4621 cgacttttcg acgtaaggag aaagaatttc aatgatagaa acaataaatc ccgcagaaaa
     4681 caaggtttct gcgtacattc atatcccatt ttgcgagcat atctgctatt actgtgattt
     4741 taataaagtt tttttagaag ggcagccagt agatgagtat gtggaaatgc tgctgaatga
     4801 aatgaaaata atgctagaaa gtcatccgac agcgaattta gaaacacttt acgtaggggg
     4861 cgggacacca acttctttat ctgctaaaca acttgatcga ctgttatcag gtgctagaga
     4921 gatcctgcct tttcaaaatg gtaatgagtt tactgtagaa gcgaatccag gtgatttgac
     4981 ccgtgaaaaa cttcaagtaa tgaaaaacta tggcgtcaat cgtttatcca tgggagtgca
     5041 gacatttgat aatcgcttat tgaaaaaaat tggacgtaaa cacaccgcag aagatgtgta
     5101 tcaaactatg caatttttag aagcagaaaa ttttaccaat gtaagcatcg atttgattta
     5161 tgcattaccg ggacaaacac tagaaggata tctggatact ttggaaaaag ctttggcttt
     5221 ggatctgcct cactattctt tgtactcatt gatcctcgaa aacaaaacaa tgttcatgaa
     5281 ctgggtacga caaggaagac tcgaactgcc tgatcaggaa acggaaacca gaatgtttga
     5341 agagacgatt gaagcaatgg aaaagcatgg acgtcaccaa tatgaaataa gtaactttgg
     5401 gctctccgga catgaatctc aacataacct catgtactgg aacaatgacc actattttgg
     5461 gtttggagct ggtgctagcg gttatcttgg agatattcgt taccgcaaca aaggaccaat
     5521 ccaacactat ttacgcccat tacgagcaaa tgaacgcccg gtattggaag aagaaaaatt
     5581 gacacgaaaa aatcaaatag aagaagaaat gttcttagga ctaagaaaaa aaacagggat
     5641 tactaaaaaa catttctatg atcgctatca aaaaacaatt gaatcggtgt acggtgatgt
     5701 gctaagggaa ttagagcaag aagggttact tgttaatgaa gaagaccgtg tttatttgac
     5761 accaagaggg acctttttgg ggaacaatgt gtttgagcgc tttttgtttg aaaaatagtt
     5821 taaagaatat ttgtttttta tctgaattta aataaaaaaa gttcaaaaat acaataaagg
     5881 actattgaaa cgttgatata actggattta catcaattat ttagaattag cactcttgtg
     5941 tttagagtgc taaaaaatgg agtctttctc ttgacaggag aagggcttct tgctatattt
     6001 ataagtggat tagcacttag aatatatgag tgctaatgag aggtgaggaa gatgttaaca
     6061 aagcgacaaa acgacatttt acgtctgatc atccaacact atacgagtag tggcgtgccc
     6121 gttggttcca aaacattgat gtctgaggga gtcaaggcca gctcagcaac gattcgcaat
     6181 gatatgaaag cattagaaga tgaaggattg ctgttgaaga ctcactcttc ttctgggcgg
     6241 attccatctg ctttaggcta tcgttattac gtggatcatt tgttaaaacc tgctcgcgta
     6301 gcagaagatg atttgctgct gattcgtcag tctctaggca gagaatttca tgaaatcaat
     6361 gagattatta agcaatctgc tgagatatta tctgagctga caagctatac gactttttca
     6421 ttgggacctg aaataaaaga ccgccgactg actggctttc gtatcgtacc cttgaataat
     6481 cgacagatta ttgctatcgt cgtaacagat atggggaatg tagaaagcca agtgttcact
     6541 cttcccgaat atatgggaag tcaggaacta gaaaaaatgg tacagattgt aaacgaccgt
     6601 ctagtcggag atcctctgat gacggtgtac cataaacttc gtacggagat tccgatgatt
     6661 cttcataaat atttccaaac gacagaaggg atgtctcagt tatttgacac cgttcttggt
     6721 catgtttttg aagataaggt ttatgtcagt ggacaaatga atctcttgga ttatgagccc
     6781 cgtcaagatc ttgatcagtt caaatcgatg ttttctttta tgaaagactc agacggactt
     6841 acacaaatga tcactccgat ggatagcaat attcatatca agattggtgc tgaattaggc
     6901 aatgatttat tgcagaatat gagtatgatt caagcaagtt acgagataga tggtcatgga
     6961 agaggtacaa tagccttgtt agggcctact agtatgcctt attctcggat gttcggtcta
     7021 gtcgatgttt atcgaagaga actagcagct aaacttgctg attactaccg ttcgcttgac
     7081 ttgtcaaact cgtaatgtga ataacgaagc tatttgcagc tgatcgttct ctctttctta
     7141 gtaagagcaa tcaaacagct gactgaataa tgaaaggaag actgtatcgt gaaagaaaat
     7201 caagaagaac tagataaaga aatgacagat gctcagccag aaccggaaat cgatgtcgaa
     7261 gctgcagaag attcgggtat ttcagaagct gaagcggaag aattcgaaac agcgaaatta
     7321 aaagctgagt tagaagaaat ggaagataaa tacttgcggg ctcgtgctga aatcgcaaat
     7381 atggcaaatc gaggaaaaaa tgagcgtgag caattacaaa aataccgttc acaagattta
     7441 gccaaaaaat tacttccatc aattgacaac ctggaacgtg cattagccac agaagtgtca
     7501 gatgatcaag gggcaggttt gaaaaaaggc gtggaaatgg tcttggaaag tttgcgtaac
     7561 gcactggaag aagaaggaat tgaaaaaatc cctgctaaag gagaagcctt tgatcctaat
     7621 cttcatcaag cagtacaaac tgttccagcg actgaagata caccagccga tacgattgta
     7681 gaagtactac aagaaggcta caaattacat gatcgcgtgt taagaccaac catggtcatt
     7741 gttgcacaat aattgtgcgg atataaaact tacactttca attaaagaag gagacgttaa
     7801 ttatgagtaa aattatcggt attgacttag gtacgactaa ctctgcagta gcagtattgg
     7861 aaggaaacga agcaaaaatc atcgctaacc cagaaggaaa ccgtacaaca ccttctgttg
     7921 tatcattcaa aaatggagaa atccaagtag gggaagttgc aaaacgtcaa gctgtgacaa
     7981 acccaaatac gatttcatca atcaaacgcc atatcggtga agcaggttac aaagtagatg
     8041 tagaaggtaa atcatataca ccacaagaaa tctcagcaat gatccttcaa tatatcaaag
     8101 gattcgcaga agactattta ggtgaaaaag tcgaaaaagc agttatcact gtgccagctt
     8161 actttaatga tgcacaacgt caagcaacaa aagatgctgg taagatcgct ggtctagaag
     8221 tagaacgtat cgtcaatgaa ccaactgcag ctgctcttgc ctatggttta gataaaacag
     8281 atcgcgacga aaaaatctta gtattcgacc ttggtggcgg tacttttgac gtatctatcc
     8341 ttgaactagg agacggtgtc tttgatgtat tgtcaacagc tggcgacaac catctaggtg
     8401 gggacgactt tgataacaaa atcatcgact acatggtagc agaattcaaa aaagaaaaca
     8461 gcattgactt gtctcaagac aaaatggctt tacaacgctt gaaagacgct gctgaaaaag
     8521 cgaaaaaaga tttatctggc gtatcaagca cacaaatcag cttgccattt atcacagcag
     8581 gagaatctgg accattgcat ttggaaatga cattgacacg tgcaaaattt gatgaattaa
     8641 cagctgactt agtagaacgt acaaaagtac cagtacgtca agcgctaaaa gatgcaggtt
     8701 tatcacaatc agaaatcgac gaagttattt tagtcggtgg atctactcgt atccctgcag
     8761 ttgtagaagc agtacgtaaa gaaacaggaa aagaaccaaa caaatcagtt aaccctgatg
     8821 aagtagttgc tatgggtgct gctatccaag gtggcgtgat cactggtgat gtgaaagatg
     8881 ttgttttact tgacgttaca ccattgtcat taggtatcga aacaatgggt ggagtgttca
     8941 ctaaattgat tgaccgcaat acaacgattc caacaagtaa atcacaagta ttctcaactg
     9001 cggcagataa tcaaccagca gtagacatcc atgtattaca aggtgaacgc ccaatggctg
     9061 ctgacaacaa aactctagga agattccaat tgacagatat tccagcagct cctcgtggta
     9121 ttccacaaat cgaagtaaca ttcgatattg ataaaaacgg gattgtaaat gtatctgcga
     9181 aagatctagg tacacaaaaa gaacaaaaaa tcacgatcaa atcttcttca ggtctaacag
     9241 acgaagaaat cgaacgtatg gtaaaagatg cagaagcaaa tgcagaagca gataaagctc
     9301 gtaaagagga agttgaccta cgtaatgatg tagatgcatt gctattctct gtggacaaaa
     9361 cattgaaaga attagaaggc aaagtggacg aagaagaagt gaaaaaagca gaaacagctc
     9421 gcgatgaatt gaaagccgct gttgaagcaa acaacatcga agaaatgaaa actaaacgcg
     9481 atgcattgaa cgaaatcgtt caaaacttga ctgttaaact atacgaacaa gcggcacaac
     9541 aacaagctca agaaaaccca gaagcagctc aaggcggagc agatgatgtc gttgatgctg
     9601 attttgaaga agtcgatggc gacgataaat aatcagttca ctgaatttcg agagaaggcc
     9661 aaagttgcta ctttggcttt ttcttttgaa aaatagtttg aagtatcggt ttttgtttca
     9721 aacaaaatat gatatgataa caagcgacgt gaaaatgatg agaaccaata ggaggaaagc
     9781 ctatggcaac aaaacgtgat tattatgaag tcttagggct ctcaaaagga gcatcagaag
     9841 atgaaataaa gaaagcttat cgtaagcttt cgaaaaaata ccatcctgac atcaataaag
     9901 aacctgatgc ggaagaaaaa ttcaaagaag tatcagaagc atatgagatt ttaagtgacc
     9961 cgcaaaaacg cgctgcatat gaccaatacg ggcatgcagg cacagatcca aattacggtg
    10021 ctggtggagc tggtggcgga tttggtggat tcggcggctt ctctggtggc ggcttcggtg
    10081 gttttgaaga tatcttcgac tctttctttg gaggaggcgg tcggtcagtc gatccaaatg
    10141 cgcctcgtca aggtgctgat ttgcaatata cggtcgactt gtcttttgaa gaagcgattt
    10201 ttggtgtaga aaaagaaatc aagtataatc gggaagaaat ctgtcatact tgtggtggga
    10261 acggtgcaaa accaggcaca caaccaacta cttgtcataa atgtcatggt gcaggtacga
    10321 ttaatgtaga acgccaaaca ccacttggac gagtgatgag ccgtcaaact tgtgatgttt
    10381 gtcatgggac agggaaagaa atcaaagaac cttgtccaac ctgtcatggc tcaggacatg
    10441 agaagaaagc acattcagtt aaagtgaatg taccagctgg tgtagaagaa ggacaacaaa
    10501 tgcgtttagc tggtcaagga gaagctggag aaaacggcgg accatttggt gatttgtatg
    10561 tggtcttccg tgtagaagaa agtgatatct ttgatcgtga aggttctgaa atttactatg
    10621 aattgccttt aagttttgta caagcagctt tgggggatga agtccaagta ccaactgtcc
    10681 atggagatgt gaaattgaag atccctgctg gaacacagac aggtacgaac ttccgtttac
    10741 gtggaaaagg cgctccgaag ttacgtggtg gtgcaacagg cgaccaacat gtcaaagtaa
    10801 aactgatcac accgaaaaat ctaaatgaac aacaaagaga agcattacgt gcattcgctg
    10861 aagctggcgg catcaaagtt gaagaacagc aagaagacgg tttcttcgat aagtttaaag
    10921 atgcttttgg cgggaaaaag cggaagtagt ttttctagta aaaacgctaa gtcattaata
    10981 attaaaaacc agacgaacaa tcatatgttc gcctggtttt tttgtagttg tcatgtttgt
    11041 accaacaaaa aagccagctt tagaatatga taatcgtcat agtttctagg ggtataacca
    11101 gtgagagaat ggaacttgtt caatcgatat tgcaaggtgt ttttatgaat aaataattct
    11161 tctgagcaat ttttgatgct tccgttattt ttactgaagc atgccaaagt cttcttgaac
    11221 aaatgtactt cttctgtcag gcttttcaaa atacgtttta catataatgt tcgttgatct
    11281 gacgggattg ctggtaaaac gatcccgagg tctaaatctt caaaaaagcg gatctcgttc
    11341 acagattgat ggaaagctat ccattcgcaa acttgtttgg catgaaggaa agattgcggt
    11401 gtctcttgct ctgtataaca aagcgaccca attccaaagt ttagctgtaa ctgatacatt
    11461 ttattaaact ttgtctggat tttatcaagc aatgatagca atgaggctct ggaaatccga
    11521 ttgactagga taatgatttg ttgtctatgt acagtgagca aagcctgtcc gtaataagag
    11581 aagacccttt cgagcaactc caataaagga ttgatccgtt cttgacacaa ttgctgattg
    11641 tcagaaagtt gtcccacaac tacataatat ggagaagaaa aatcaagctc aattaagtga
    11701 gggtggtatc gactatgtac ttgatatcat caatgaaccg atggtacttt cttatgcgat
    11761 ggaacaggta gagcgattgc ttgtttgtgc aggaaaacaa gcgatgcggc tgatacgatt
    11821 aggtgagaaa ataagaagat aaaaatacac ggtcccttca tggcgaccgt gcatttttgg
    11881 tttttattaa atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt
    11941 ttcaaagatt tgttgaggcg ttccatcttc taaaaagttt ccgtcgtcaa tgaacatgac
    12001 gcgattggcc acttctttag cgaaccccat ttcgtgcgta acgataacca tcgtcatacc
    12061 ttgttctgct aactttttca tgacgttcaa gacatcaccg accatttcag gatcgagtgc
    12121 agaagtaggc tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc
    12181 cacacgctgt ttttgtccac cagaaagcat ttcaggatac atatcttttt tatcttctag
    12241 accaactgtc tgcagtaatt tgattgcttt tttctctgct tcttctctag attctttctt
    12301 caggtccatc ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa
    12361 gtgttggaat accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat
    12421 caagtgggcg ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca
    12481 acgcaagaat gtactttttc ctgaacctga agggccaatg atacagacga catctccttc
    12541 gttgatggat acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg
    12601 ctcaactaag attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt
    12661 ttgtcaatgc tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac
    12721 tttgtgttgt acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg
    12781 cggaaatgat cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca
    12841 tgatgcgtat cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta
    12901 aacttctgga agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct
    12961 ctgcgatata agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa
    13021 tcgtaaatcc aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg
    13081 gaattcctcg gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg
    13141 ctacactgaa taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag
    13201 ttaggagaat tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc
    13261 caagaaatgt tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta
    13321 catattcttt aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat
    13381 tgaacatatt aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc
    13441 cagattcttt tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta
    13501 ccggataatc atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat
    13561 cagcagcatc aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt
    13621 cagcactttc ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact
    13681 tgatcgaagt atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag
    13741 aaaaatcata gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg
    13801 cttgtcctga ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt
    13861 taaatccttg cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc
    13921 cctttgcgtt ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt
    13981 cttttttggg ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt
    14041 tgtcagcttg tgcaacatga ccaaacgcaa gtgtcacaag tcctgtcatt aagaaaacaa
    14101 atgagtgaaa taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta
    14161 ttataggcga aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc
    14221 gagtttctaa cattttatgt tataaaacat aacatgtata ataagataaa aaagtggcga
    14281 aagtatgttc ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt
    14341 tattggtcgt aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga
    14401 tacctcccga cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc
    14461 gattaaccaa ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc
    14521 gactggaaca gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac
    14581 actgattatt gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt
    14641 tttccctaac aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc
    14701 ttatgtgcct tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga
    14761 caaattacga cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc
    14821 atccgtttca tgtatttttg gattaggctc gccttttgag taccaaaaac aagtagtttc
    14881 gattcgacag ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca
    14941 attcgaaaga aatgatatag attttcagcg cggtagtttt cgagtgcgcg gtgatgtcgt
    15001 ggagattttt ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga
    15061 ggtcgaacgt atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca
    15121 cgtagcaatc ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt
    15181 agcgaatatc aaagaggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact
    15241 tctggaagct caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat
    15301 gggctacacg tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga
    15361 accgccttat acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc
    15421 gcacgtaacc atgcctcaaa tcagaggaat gtataatggt gaccgagcga gaaaacagat
    15481 gctagtagat tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga
    15541 agagtttgaa aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga
    15601 acatgaacaa acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc
    15661 agtgatcgaa gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga
    15721 acgagtcgaa aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga
    15781 cctcacagat tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa
    15841 aacgctggaa cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt
    15901 tggaatcaat ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt
    15961 ggatgcggat aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg
    16021 agctgctcgg aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat
    16081 gcgtctagcg atggatgaaa cgtcaagacg tcgtacgatt cagcagaagt acaatgaaga
    16141 acatgggatc gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa
    16201 agaatcggaa gatgatacaa aagaagcggt acaagtctcg tacgaagaaa tgacgaagga
    16261 agaaaaagac acgttattga tgaaattaga aaaagaaatg aaagatgcgg caaaagcttt
    16321 ggattttgaa acagccgcga atgtgcgaga tatgatttta gaattaaaag cttcaaagta
    16381 ggaggacaac atggctaacg ataaaattac gattcatggt gcacgtgccc ataacttgaa
    16441 aaatatagat gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc
    16501 tggaaaaagt tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga
    16561 gagcctttct gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag
    16621 catcgacgga ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg
    16681 gtcgacagta ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt
    16741 aggacatccg atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat
    16801 ggcagatcaa gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt
    16861 ggtaaagaaa aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt
    16921 ccgcgttcga gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa
    16981 caaaaaacat gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc
    17041 tcgcctattt gattctttag aggcggccct gcgattagca gacggctatg ctctggttga
    17101 cgtgatcggt gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt
    17161 tactgttggc gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc
    17221 agactgtgat ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag
    17281 aacgaagaca cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta
    17341 tcctcaaatg ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga
    17401 agaattaccg gaagagcatc aggagatcat cttgaatggg acagatgatc ttttccattt
    17461 ccattatgaa aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt
    17521 gaagaatatc gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg
    17581 tctttatatg acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc
    17641 tctatctgtt caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa
    17701 agcagtagat ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc
    17761 gattttaaaa gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct
    17821 aactttgagt cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc
    17881 tacacaaatt ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg
    17941 attgcatcaa cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg
    18001 caacaccctc gttgtagtcg aacatgatga agatacgatg tgggcagcag attatttgat
    18061 tgacgtcgga cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga
    18121 agaagtagct aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat
    18181 ccctgtacca aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc
    18241 agaaaacaat ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac
    18301 aggtgtgtct ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc
    18361 gcaaaaatta aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga
    18421 aagtatcgaa aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa
    18481 tccagcgact tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga
    18541 ggcaaaaatg cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg
    18601 tgaagcttgt cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta
    18661 tgttccttgt gaagtatgtc atggtaaacg atataattct gaaacattag aagtgcatta
    18721 caaaggaaaa agcattgctg atattttgga aatgacagta gaggatgctg tagaattctt
    18781 caagcacatt ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta
    18841 tgtgacgatg gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact
    18901 tgccagtgaa ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac
    18961 gacaggactt catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga
    19021 tgctggtaac acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta
    19081 tatcattgat ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac
    19141 accagaagaa atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat
    19201 ggtataaaga ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct
    19261 tgttttatta atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg
    19321 tatagataaa aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga
    19381 ctttcctttg attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa
    19441 aacaagagat tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta
    19501 tccctatatt attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag
    19561 catacggcaa aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta
    19621 aaattgtcaa tgtagaagtt attccaggat ttgataaaga ccctgtattt atcgagacga
    19681 tgcttcgttt ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt
    19741 gtggtgatgg atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta
    19801 tctgtccata tattgattat tctttatttg aaaggctgat caataaagta agcttttatg
    19861 aagtttgtga agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt
    19921 tagtaaatgg acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg
    19981 caaacagtgt ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag
    20041 agactagaga agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg
    20101 aaatgattgt acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt
    20161 atgtagatga agatcatcaa gttcggatga tgtgccttgg tcatccacta ttagaagatc
    20221 ctacaccagc atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct
    20281 atcaaaccat taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata
    20341 tgaagtatga tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc
    20401 gtagcagttt ctttgtgaca ttgaacggct tgaatctggc tcgttttgtc acagaagatc
    20461 gagtgttcaa caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa
    20521 gattatggat gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca
    20581 aaaaactagc tgagcaaatg atcaaagaaa atcgttacgg tactactgta ttttatgaaa
    20641 aagaccgatc aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa
    20701 gattcaaaaa gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt
    20761 taggcggaat gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga
    20821 aagctacaaa agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg
    20881 atcgtacagc ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg
    20941 atattgagaa acaaaattta ttgcgaccaa acttcattgt gttgacttgt aacaccgcac
    21001 actacttttt tgaagagtta caagcggcaa cggatattcc tatcttgcat atgccaagag
    21061 aagcagcaaa tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag
    21121 aaggaagtat gaaagctgga atctatgaac gcgaagtcaa aaatcttggt ttcgaaacag
    21181 tgattcctga tactgcgctt caagaaaaaa tcaattacct gatctatcat gagatcaagg
    21241 aatcggatta tttgaatcaa gagctctatt acgaaatctt agaggaagcg gtagagcgcc
    21301 taaactgtga aaaagtcatc ctaggttgca cagaattatc tttgatgcat gaatttgctg
    21361 aagataatca ttacccagtg atcgatgctc aatctatttt agcggatcga acgatcgaac
    21421 gtgcattagc tgaaagaagc gaggcattgg atacagcatc ggaaaagtaa aaaaattgga
    21481 tttggggcaa atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa
    21541 ctggtaatga tacgcccggg atgatataat tttattatgc acaatgtgag aggggttcgt
    21601 aaatatgaca gataatttgc aattagtcat cataacaggt atgagtggtg cagggaaaac
    21661 agtagctatc caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg
    21721 attgattcca aaattctggg aattgatcaa agaatcaggt aaagtcacaa aaatggcttt
    21781 aggagtggat ttgcgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat
    21841 cgaaaatacg aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact
    21901 agtggctcgc tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac
    21961 tgaagggata cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat
    22021 cattgatacg acaacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa
    22081 atcaaaggga gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt
    22141 gccgatcgat tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc
    22201 ggaactaaga ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga
    22261 tgaaacgcaa tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata
    22321 cattaaagaa ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg
    22381 ttctgtggca ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt
    22441 gacacatcgg gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc
    22501 taccggataa gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc
    22561 ctgaaaagtt tacgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat
    22621 ggtggaagca gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga
    22681 aatgtcttag tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt
    22741 ttcaaaaagg aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca
    22801 gtttccgaga tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat
    22861 gtagatggac atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtttttaaa
    22921 gacggaacga ctgcagtagg agaatctaaa atcgcgatca accgtaaaac cattgaccgt
    22981 gtgtatgtct gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca
    23041 gcaattatgg acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt
    23101 ccaaatttag tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc
    23161 tatatctgta atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat
    23221 atacgtgttt tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg
    23281 gaaaaagtgc ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc
    23341 gcacacgact tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgacttttta
    23401 gaattgaaag atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt
    23461 ttagttttcg gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg
    23521 tcatttgctg cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg
    23581 aaagcggaat tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag
    23641 tttattttga acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg
    23701 aaagatcatt atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag
    23761 aacaatgtct atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat
    23821 atcatggatg gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa
    23881 aaaatgcgtt cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa
    23941 acgagtcgtt atcatttgga aatattttct atttatgaac agcataacca agacatttgc
    24001 gatatgttaa attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt
    24061 tcgtatttaa aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac
    24121 tcaatgctcc gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt
    24181 ctagtcaatt gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc
    24241 gaaaacatcg aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag
    24301 gaaatcgcag aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg
    24361 attccctcag gtgcaatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa
    24421 tttgcggaaa aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc
    24481 aggagatgcg aatgtatcag ttttttttaa aagctatact tgcttttctt ccgtcaaact
    24541 ataattccaa ctttactgga gtaacataat gttgaaaggc ttctattgtt ttttaataca
    24601 atcgtataaa aagcggggag acaactgaat gaagatttta gttttataca taaacttttg
    24661 cttaatatag aagtcctgat atagatagga tcaagtattt atctataact ggagattaat
    24721 taaaaactgg aaggaactgc tcgatcaacc ttgagaactg tttttcttac ttctggtcaa
    24781 gattctcgtg gttggtggat aaggatggtt gataagttca ttggattagc tagtcgaaaa
    24841 caaccaggtg taattaatgt agaagtttta acaaggggat ggagtgattt attcagtgat
    24901 aatcataagt tcataaatag taattgggat gtatggttgg tatacgttat acctgaactt
    24961 gaactatcaa gatttggttt agaatttgag aatcgatgtt tataaaggga gggcaaaaaa
    25021 tgtgcttttt tacaactttt actatcatta tgattgcagt actttttatt tttctagata
    25081 tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca ggtctcataa
    25141 catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt tggagaacta
    25201 cagcaccacc attgccagag attacatatg gagaatttcc gtttagtctt gaatatgaaa
    25261 tagatgagga aagaattttg attgaagata cggtgattgc agaatttata ggaagtttta
    25321 gagggaatgt gacaactcaa cctagacgaa gttggagtat aaaactttta aataatagtg
    25381 gagagttgaa agcatttcat tatgaattct taataataga aagtgaagat gtaacaatca
    25441 ctttaagtag tggctctgct ggatatttta tgaatgatgg tggaagcaag ccacagctca
    25501 cagtccgtga tcgtattgca ggttttatgc atttttctca ctatgactta gaagaagctc
    25561 gtaaaatact tgcagaatat gggatcacat taatcaattg ggaatatgcg ccaccgataa
    25621 gaaatttttt caatgattat tattagtctt ataaaaaaca gaatgtaaaa tttatgtgcg
    25681 cgagtattta gttttccatt ttttgtttga tagatatata gcattgttga agcatgggat
    25741 agtggtgcca attttatcaa tgaggttggt caggcagtta acataattgg tgatgctata
    25801 aatccattta attggaattg gaggaattag gtgtggaaac agtgagatta tggatattca
    25861 tgaaaaattt tcgttcggct ttgagatatg ttattctgga atcaaaatca ggttattatc
    25921 tgattgacac agatcactat actttttggg gaggctacct ttttccgatt tttaattgga
    25981 taatccccca taaggttatg aaaattaact tgcctgcaga agaaataaat agattaatac
    26041 tcaacaaaga tttacaaagg aaaagagaga aagaacattc taatgttgct tggataggtc
    26101 cagcatttgc agtagtgact tcttcaattg tcggtcctat gctagagagt atttatagtg
    26161 atttttctat tggaatacgt tatttatttg ttactttaag tatagctgtt cttttgtgca
    26221 taaagggtta tgttagcaag aagaataaag atattttaac actaatcggt gtaaacaatt
    26281 tatctagtac taaaattata ctgtttcctg aatctataaa agaaggatca aggttgttga
    26341 taatttttat aatgcttata ctgttttctg ttttatttgg ggtgatgttt ataga
//
LOCUS       DACCWK010000030        25583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCWK010000030 DACCWK010000000
VERSION     DACCWK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.030"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            233..787
                     /locus_tag="IVW65_001192"
     CDS             233..787
                     /locus_tag="IVW65_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7900107.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(960..1433)
                     /locus_tag="IVW65_001193"
     CDS             complement(960..1433)
                     /locus_tag="IVW65_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7900108.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1445..1981
                     /locus_tag="IVW65_001194"
     CDS             1445..1981
                     /locus_tag="IVW65_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7900109.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2021..2392
                     /locus_tag="IVW65_001195"
     CDS             2021..2392
                     /locus_tag="IVW65_001195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7900110.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2676..3947
                     /locus_tag="IVW65_001196"
     CDS             2676..3947
                     /locus_tag="IVW65_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP7900111.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4192..5217
                     /locus_tag="IVW65_001197"
     CDS             4192..5217
                     /locus_tag="IVW65_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7900112.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5511..6620
                     /locus_tag="IVW65_001198"
     CDS             5511..6620
                     /locus_tag="IVW65_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7900113.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6703..7599
                     /locus_tag="IVW65_001199"
                     /pseudo
     CDS             6703..7599
                     /locus_tag="IVW65_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7615..8439
                     /locus_tag="IVW65_001200"
     CDS             7615..8439
                     /locus_tag="IVW65_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7900114.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8471..9781
                     /locus_tag="IVW65_001201"
     CDS             8471..9781
                     /locus_tag="IVW65_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP7900115.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9929..11188
                     /locus_tag="IVW65_001202"
     CDS             9929..11188
                     /locus_tag="IVW65_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7900116.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11198..12070
                     /locus_tag="IVW65_001203"
     CDS             11198..12070
                     /locus_tag="IVW65_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7900117.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12082..12912
                     /locus_tag="IVW65_001204"
     CDS             12082..12912
                     /locus_tag="IVW65_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7900118.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12952..15135
                     /locus_tag="IVW65_001205"
     CDS             12952..15135
                     /locus_tag="IVW65_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7900119.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15196..16416
                     /locus_tag="IVW65_001206"
     CDS             15196..16416
                     /locus_tag="IVW65_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP7900120.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16413..17873
                     /gene="gtfA"
                     /locus_tag="IVW65_001207"
     CDS             16413..17873
                     /gene="gtfA"
                     /locus_tag="IVW65_001207"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP7900121.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17950..19056
                     /locus_tag="IVW65_001208"
                     /pseudo
     CDS             17950..19056
                     /locus_tag="IVW65_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19378..19587
                     /locus_tag="IVW65_001209"
     CDS             19378..19587
                     /locus_tag="IVW65_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7900122.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19580..19729
                     /locus_tag="IVW65_001210"
     CDS             19580..19729
                     /locus_tag="IVW65_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7900123.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19764..20548
                     /locus_tag="IVW65_001211"
                     /pseudo
     CDS             19764..20548
                     /locus_tag="IVW65_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20832..21917)
                     /gene="xerS"
                     /locus_tag="IVW65_001212"
     CDS             complement(20832..21917)
                     /gene="xerS"
                     /locus_tag="IVW65_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7900124.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22251..22484
                     /locus_tag="IVW65_001213"
     CDS             22251..22484
                     /locus_tag="IVW65_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP7900125.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22481..22888
                     /locus_tag="IVW65_001214"
     CDS             22481..22888
                     /locus_tag="IVW65_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7900126.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24025..24543)
                     /locus_tag="IVW65_001215"
     CDS             complement(24025..24543)
                     /locus_tag="IVW65_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7900127.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8711 a         3907 c         4769 g         8196 t
ORIGIN      
        1 cggataactg cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta
       61 atttttttgt acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa
      121 ctaaatccct tgattttatt gactttcaaa agtttatata tataagttaa tgattatggt
      181 aaaatatata cataatcatt aactcagaat atttttaaga agggaaacaa tagtgagcta
      241 taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag
      301 aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata atgggttacg
      361 tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta agcctattat
      421 tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga gagaagaaat
      481 tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc ggcaacaagg
      541 tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg
      601 acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa
      661 aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa
      721 aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat ttaaactagg
      781 tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta tttttatcta
      841 gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt acttggtata
      901 ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa aggatgaaat
      961 taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact
     1021 gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc gtttttgacc
     1081 atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat gaaatctgca
     1141 aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat atcatccata
     1201 gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga cgcaccagat
     1261 tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta
     1321 ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag gggcttattt
     1381 tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct catttttaga
     1441 tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc gaccgacatt
     1501 tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta atcaagacga
     1561 tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa ctgaactgac
     1621 tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga ttgaacgaac
     1681 gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt ttgaaacaca
     1741 aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac gttttgaagg
     1801 gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata ttgatgatta
     1861 tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa ttgatgccat
     1921 tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg taggctggta
     1981 gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct tagaaaatca
     2041 agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc cagttgccat
     2101 tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat ctgatttaca
     2161 acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa aagatcgtga
     2221 attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg
     2281 ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg aagaaattcc
     2341 tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat aattagctaa
     2401 tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa gaaaaacgca
     2461 tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa actatcattt
     2521 tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt tatacctctt
     2581 tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt
     2641 tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg caagagaaac
     2701 aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt accagcgtat
     2761 ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct ggtcgtcaac
     2821 tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt cgtccagagg
     2881 ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag attggtggca
     2941 gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt gtgttgtttt
     3001 catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat gatcctagtg
     3061 gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata ggattgcttt
     3121 atttttcggc tattgataat gctgattttg tattttctga tccatttacc gcagaagttt
     3181 tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg cataatgtta
     3241 attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg ttcattgttg
     3301 ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt cctgttgttt
     3361 atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa atgaatcgtt
     3421 tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat ttgattcatt
     3481 atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa ccaagtgtta
     3541 ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta acctttaaat
     3601 cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta gctgaattta
     3661 ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt ttaaaaaata
     3721 agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt acttttgcag
     3781 aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag ggggcgattg
     3841 cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa gtactactat
     3901 tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa agctctgtga
     3961 aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg tgatctagca
     4021 gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt cggatatttt
     4081 ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt attttcaaaa
     4141 tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat tatggtagga
     4201 attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat
     4261 gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga atcggttaat
     4321 ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat tgcattaata
     4381 acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat tcgcagaggg
     4441 attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg tttgtcagaa
     4501 acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg
     4561 ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt gattgatgac
     4621 cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc aacgaatcag
     4681 catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg aggaggacgt
     4741 gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg aaaaatagcc
     4801 tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta tttagaaggc
     4861 tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca tgctaaagat
     4921 aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt ttatcgttca
     4981 cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt tgataatatt
     5041 gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac agaagaaatt
     5101 ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc
     5161 aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat cgattaaaaa
     5221 agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc
     5281 tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa
     5341 caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa
     5401 ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa
     5461 ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg
     5521 aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat
     5581 attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc
     5641 actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta
     5701 tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc
     5761 ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca
     5821 aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac
     5881 tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac
     5941 atattggaac agagactggc tactggccat ttatttatca aaatggggga gaagtatttg
     6001 ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt
     6061 atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg
     6121 ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg
     6181 caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta
     6241 agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa
     6301 aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa
     6361 tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg
     6421 cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc
     6481 gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta
     6541 ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg
     6601 cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt
     6661 tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt
     6721 ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta
     6781 ggattaattt tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa
     6841 acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca
     6901 gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct
     6961 agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg
     7021 tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg
     7081 atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata
     7141 ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt
     7201 gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa
     7261 attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt
     7321 ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt
     7381 atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc
     7441 atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac
     7501 aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca
     7561 attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa
     7621 aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg
     7681 gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa
     7741 attccaccta caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat
     7801 ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt
     7861 ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg
     7921 aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt
     7981 actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata
     8041 ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta
     8101 cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc
     8161 aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg
     8221 tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct
     8281 ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg
     8341 gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa
     8401 ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga
     8461 gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa
     8521 cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga
     8581 tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc
     8641 gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc
     8701 tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt
     8761 tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg
     8821 gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga
     8881 agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg
     8941 tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt
     9001 accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg
     9061 gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga
     9121 tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt
     9181 acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc
     9241 agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat
     9301 tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg
     9361 agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata
     9421 tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa
     9481 aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa
     9541 aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg
     9601 gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa
     9661 aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat
     9721 caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta
     9781 aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag
     9841 taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc
     9901 attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg
     9961 tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag
    10021 cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga
    10081 gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc
    10141 gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa
    10201 tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt
    10261 aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga
    10321 ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac
    10381 tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt
    10441 agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac
    10501 tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa
    10561 caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa
    10621 agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg
    10681 tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa
    10741 tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt
    10801 tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt
    10861 atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac
    10921 aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg
    10981 cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg cttttactga
    11041 tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac
    11101 aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt
    11161 taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa
    11221 tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta
    11281 ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa
    11341 ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa
    11401 atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg
    11461 tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg
    11521 gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt
    11581 taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct
    11641 tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat
    11701 tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga
    11761 agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta
    11821 cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt
    11881 tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt
    11941 acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt
    12001 agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt
    12061 tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg
    12121 cttttattat tagggggctt catgatttta gttcccttag tttcaacgct attcggttca
    12181 tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg
    12241 gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc
    12301 atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta
    12361 gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga
    12421 atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt
    12481 ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta
    12541 tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa
    12601 attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca
    12661 atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca
    12721 ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca atataattat
    12781 acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc
    12841 acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga
    12901 gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata
    12961 tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta tctattagga
    13021 atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac gcactattct
    13081 ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa
    13141 gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat
    13201 tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt
    13261 tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat
    13321 acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga
    13381 ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg
    13441 caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt
    13501 gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt
    13561 caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca
    13621 agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa
    13681 ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa
    13741 gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa
    13801 ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt
    13861 ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac
    13921 tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt
    13981 aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt
    14041 gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg
    14101 gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat
    14161 aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta
    14221 tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga
    14281 aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg
    14341 acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg
    14401 acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat
    14461 atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt
    14521 gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa
    14581 acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc
    14641 ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca
    14701 ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat
    14761 gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc
    14821 ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg
    14881 tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc
    14941 ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa attagcagga
    15001 ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg
    15061 aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat
    15121 ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt
    15181 ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat
    15241 tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa
    15301 ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc
    15361 atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag
    15421 tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt
    15481 atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat
    15541 ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt
    15601 gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat
    15661 caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca
    15721 cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg
    15781 gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga
    15841 atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag
    15901 tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa
    15961 cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc
    16021 tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa
    16081 gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga
    16141 aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa
    16201 aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta
    16261 aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc
    16321 aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa
    16381 agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca
    16441 tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac
    16501 atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc
    16561 gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg
    16621 tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc
    16681 gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa
    16741 tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt
    16801 taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga
    16861 cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag
    16921 ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa
    16981 ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg
    17041 aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg
    17101 aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt
    17161 attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat
    17221 cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata
    17281 cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt
    17341 acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca
    17401 attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata
    17461 atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa
    17521 tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag
    17581 aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac
    17641 ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg
    17701 atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta
    17761 aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta
    17821 tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc
    17881 catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag
    17941 gactcataca tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata
    18001 aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt
    18061 aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa
    18121 gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat
    18181 gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg
    18241 ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag
    18301 cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag
    18361 tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg
    18421 gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc
    18481 aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat
    18541 taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt
    18601 aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt
    18661 ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta
    18721 gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc
    18781 tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag
    18841 cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag
    18901 aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg
    18961 aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc
    19021 tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac
    19081 ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc
    19141 agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt
    19201 ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt
    19261 aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa
    19321 cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg
    19381 gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga
    19441 atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta cgaaaataga
    19501 agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta
    19561 gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt
    19621 tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg
    19681 gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag
    19741 aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac
    19801 cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct acaatcaaaa
    19861 aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg acattcaaaa
    19921 gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca tgtatgggat
    19981 ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa
    20041 agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa aagaagaatt
    20101 tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg gttttatttg
    20161 tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat taacttggaa
    20221 agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt ataagagagc
    20281 caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa tcgaactaga
    20341 cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa taggaggcat
    20401 gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc gtcatattat
    20461 gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat taaggcatag
    20521 tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag aaaggttaaa
    20581 acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa attcattgag
    20641 agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa tcaaaccgac
    20701 acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac caaaagaaac
    20761 acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt ccgcctattt
    20821 atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc tatatgggta
    20881 tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa taccaatgaa
    20941 tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg gctgacttga
    21001 attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc ggatatacgc
    21061 ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt tactttatat
    21121 cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc tgctatattg
    21181 acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc taaagatagg
    21241 tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa aatcagtgca
    21301 ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg tttactcaaa
    21361 gtatttttat attcattggc aatataatct agataatttt gtgtatcctt atctagaaat
    21421 aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt gatggttgct
    21481 acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt ggaacttttc
    21541 gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt agaagtagat
    21601 agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt tacattttct
    21661 tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt taactgtctt
    21721 atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc ataaaagagc
    21781 ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc ctcacgatct
    21841 tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat tttttgatat
    21901 atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga gtatctctat
    21961 aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa atttattaat
    22021 ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata aatggtatac
    22081 ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc ttaattaatt
    22141 atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc ttgatttaaa
    22201 atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact atggcaatta
    22261 aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca aaggagtttc
    22321 ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa aaacttcgag
    22381 cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata tctcaagcat
    22441 taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc attacctaga
    22501 agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc
    22561 ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc gagcctcagc
    22621 ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac ttatacaatt
    22681 gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag ctacttttgt
    22741 ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg taaatttagc
    22801 ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag tgatcaacac
    22861 tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta tagggttatt
    22921 agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa atccaactat
    22981 atagaaaacg taaattttct atatagttgg attaagataa cgactattta ttttatcata
    23041 tttttactat accctatact tttacttggt atatttatga ataatcgcaa atttacccct
    23101 atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt cttttattaa
    23161 atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata aaaataacag
    23221 ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac
    23281 ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat cgttactggg
    23341 attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta ctatttgatt
    23401 tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt atagaattta
    23461 tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc ctttgatggt
    23521 tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa caaaggctat
    23581 ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc ttcctaaccc
    23641 aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg ttgagattcc
    23701 atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa
    23761 atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca taatctcatt
    23821 ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa tctaacgatg
    23881 caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa ccggcagtta
    23941 gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc tcctttccac
    24001 aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc ttatctttgt
    24061 tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata cttcccatac
    24121 agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata gatcttcatc
    24181 actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa tttcctctaa
    24241 ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact ctcctaactg
    24301 attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt catcattttc
    24361 ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag caggtgttct
    24421 atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa attctaaaat
    24481 atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt tatatgttct
    24541 cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata atttcatcca
    24601 cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg
    24661 tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg tcagcaagga
    24721 gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc atatcatttt
    24781 cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac taccacttta
    24841 agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc attgttaaac
    24901 ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg ctctttcttc
    24961 gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac gttccttacg
    25021 gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa tttaattcta
    25081 aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca agatttacct
    25141 gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat ttataatact
    25201 acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca gatacacttg
    25261 caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa tcttccgaaa
    25321 tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa
    25381 ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt tgacacggtg
    25441 tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct aaaaagtcta
    25501 tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca acttatatgt
    25561 tttagtgaac tacccccact tag
//
LOCUS       DACCWK010000031        25302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCWK010000031 DACCWK010000000
VERSION     DACCWK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.031"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            12..84
                     /locus_tag="IVW65_001216"
     tRNA            12..84
                     /locus_tag="IVW65_001216"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IVW65_001217"
     tRNA            88..160
                     /locus_tag="IVW65_001217"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IVW65_001218"
     tRNA            168..249
                     /locus_tag="IVW65_001218"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IVW65_001219"
     tRNA            267..339
                     /locus_tag="IVW65_001219"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IVW65_001220"
     tRNA            351..422
                     /locus_tag="IVW65_001220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IVW65_001221"
     tRNA            439..524
                     /locus_tag="IVW65_001221"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IVW65_001222"
     tRNA            535..608
                     /locus_tag="IVW65_001222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IVW65_001223"
     tRNA            618..691
                     /locus_tag="IVW65_001223"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IVW65_001224"
     tRNA            711..783
                     /locus_tag="IVW65_001224"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IVW65_001225"
     tRNA            811..884
                     /locus_tag="IVW65_001225"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IVW65_001226"
     tRNA            898..971
                     /locus_tag="IVW65_001226"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IVW65_001227"
     tRNA            995..1084
                     /locus_tag="IVW65_001227"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IVW65_001228"
     tRNA            1097..1170
                     /locus_tag="IVW65_001228"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IVW65_001229"
     tRNA            1189..1264
                     /locus_tag="IVW65_001229"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IVW65_001230"
     tRNA            1266..1336
                     /locus_tag="IVW65_001230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IVW65_001231"
     tRNA            1350..1423
                     /locus_tag="IVW65_001231"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IVW65_001232"
     tRNA            1448..1535
                     /locus_tag="IVW65_001232"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IVW65_001233"
     tRNA            1557..1628
                     /locus_tag="IVW65_001233"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IVW65_001234"
     tRNA            1651..1740
                     /locus_tag="IVW65_001234"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IVW65_001235"
     tRNA            1753..1826
                     /locus_tag="IVW65_001235"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IVW65_001236"
     tRNA            1845..1917
                     /locus_tag="IVW65_001236"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IVW65_001237"
     tRNA            1921..1993
                     /locus_tag="IVW65_001237"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IVW65_001238"
     tRNA            1998..2068
                     /locus_tag="IVW65_001238"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IVW65_001239"
     tRNA            2081..2154
                     /locus_tag="IVW65_001239"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IVW65_001240"
     tRNA            2166..2237
                     /locus_tag="IVW65_001240"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IVW65_001241"
     CDS             2503..3696
                     /locus_tag="IVW65_001241"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7900128.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IVW65_001242"
     CDS             3826..5298
                     /locus_tag="IVW65_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7900129.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IVW65_001243"
     CDS             5381..6169
                     /locus_tag="IVW65_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7900130.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IVW65_001244"
     CDS             complement(6236..6904)
                     /locus_tag="IVW65_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7900131.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IVW65_001245"
     CDS             7019..8731
                     /locus_tag="IVW65_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900132.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="IVW65_001246"
     CDS             8734..10503
                     /locus_tag="IVW65_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900133.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="IVW65_001247"
     CDS             complement(10708..11274)
                     /locus_tag="IVW65_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7900134.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="IVW65_001248"
     CDS             complement(11274..12230)
                     /locus_tag="IVW65_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7900135.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="IVW65_001249"
     CDS             complement(12230..12886)
                     /locus_tag="IVW65_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7900136.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="IVW65_001250"
     CDS             13256..15274
                     /locus_tag="IVW65_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7900137.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15701..18115
                     /locus_tag="IVW65_001251"
     CDS             15701..18115
                     /locus_tag="IVW65_001251"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7900138.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="IVW65_001252"
     CDS             18463..20016
                     /locus_tag="IVW65_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7900139.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="IVW65_001253"
     CDS             complement(20397..22181)
                     /locus_tag="IVW65_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900140.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="IVW65_001254"
     CDS             complement(22196..23917)
                     /locus_tag="IVW65_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900141.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="IVW65_001255"
     CDS             24017..24778
                     /locus_tag="IVW65_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7900142.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7825 a         4647 c         5377 g         7453 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    25261 tttgcttata aaataattat aagtatttag agatttttct tc
//
LOCUS       DACCWK010000032        24239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCWK010000032 DACCWK010000000
VERSION     DACCWK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.032"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(90..1385)
                     /gene="purB"
                     /locus_tag="IVW65_001256"
     CDS             complement(90..1385)
                     /gene="purB"
                     /locus_tag="IVW65_001256"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7900143.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1398..2531)
                     /gene="purK"
                     /locus_tag="IVW65_001257"
     CDS             complement(1398..2531)
                     /gene="purK"
                     /locus_tag="IVW65_001257"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7900144.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2515..3003)
                     /gene="purE"
                     /locus_tag="IVW65_001258"
     CDS             complement(2515..3003)
                     /gene="purE"
                     /locus_tag="IVW65_001258"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7900145.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3419..4726)
                     /locus_tag="IVW65_001259"
     CDS             complement(3419..4726)
                     /locus_tag="IVW65_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7900146.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4729..5310)
                     /locus_tag="IVW65_001260"
     CDS             complement(4729..5310)
                     /locus_tag="IVW65_001260"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7900147.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5426..5523)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5882..7174)
                     /locus_tag="IVW65_001261"
     CDS             complement(5882..7174)
                     /locus_tag="IVW65_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7900148.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7171..7794)
                     /locus_tag="IVW65_001262"
     CDS             complement(7171..7794)
                     /locus_tag="IVW65_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900149.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7797..8633)
                     /locus_tag="IVW65_001263"
     CDS             complement(7797..8633)
                     /locus_tag="IVW65_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7900150.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8650..9537)
                     /locus_tag="IVW65_001264"
     CDS             complement(8650..9537)
                     /locus_tag="IVW65_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7900151.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9553..10836)
                     /locus_tag="IVW65_001265"
     CDS             complement(9553..10836)
                     /locus_tag="IVW65_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7900152.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11519..12202)
                     /locus_tag="IVW65_001266"
     CDS             complement(11519..12202)
                     /locus_tag="IVW65_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7900153.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12220..12837)
                     /locus_tag="IVW65_001267"
     CDS             complement(12220..12837)
                     /locus_tag="IVW65_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7900154.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12847..13671)
                     /locus_tag="IVW65_001268"
     CDS             complement(12847..13671)
                     /locus_tag="IVW65_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7900155.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13664..14224)
                     /locus_tag="IVW65_001269"
     CDS             complement(13664..14224)
                     /locus_tag="IVW65_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900156.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14398..15354
                     /locus_tag="IVW65_001270"
     CDS             14398..15354
                     /locus_tag="IVW65_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7900157.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15659..16615)
                     /locus_tag="IVW65_001271"
     CDS             complement(15659..16615)
                     /locus_tag="IVW65_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7900158.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16719..17321
                     /locus_tag="IVW65_001272"
     CDS             16719..17321
                     /locus_tag="IVW65_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7900159.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17409..18908)
                     /locus_tag="IVW65_001273"
     CDS             complement(17409..18908)
                     /locus_tag="IVW65_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7900160.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18924..19448)
                     /locus_tag="IVW65_001274"
     CDS             complement(18924..19448)
                     /locus_tag="IVW65_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900161.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19746..20495
                     /locus_tag="IVW65_001275"
     CDS             19746..20495
                     /locus_tag="IVW65_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7900162.1"
                     /translation="MNRSCVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20486..21271
                     /locus_tag="IVW65_001276"
     CDS             20486..21271
                     /locus_tag="IVW65_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7900163.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21240..21956
                     /locus_tag="IVW65_001277"
     CDS             21240..21956
                     /locus_tag="IVW65_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7900164.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22058..23077)
                     /locus_tag="IVW65_001278"
     CDS             complement(22058..23077)
                     /locus_tag="IVW65_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7900165.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23096..24154)
                     /locus_tag="IVW65_001279"
     CDS             complement(23096..24154)
                     /locus_tag="IVW65_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7900166.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7176 a         5226 c         4223 g         7614 t
ORIGIN      
        1 ctaaaagaag cagaaccgct aacggatctg cttctttttt ggttattttt gctattctat
       61 tttaattgaa ttttgcaact cactcttctt tattccaaac ctactcgttt gaatatcacg
      121 tcaacgtttt tcaaatgatg atgataatca aaggcatcgt ctatttcttc tttagataat
      181 accgatgtga tggttggatc ttcttctaac agcgggcgga actgcacttg gttgtcccaa
      241 gcgtacgcag tttttggctg aacaagatca taagcttctt ctcttgtcat tcccttatcg
      301 atcaatttca gcataacgcg ctggctataa atcaaaccaa atgttgcaga catattccgc
      361 ttcatgtttt caggaaagac cgttagattt ttaacgatat tgccaaaacg atttaacatg
      421 taattcaata aaatcgtggt atcaggcaag atgattcgtt ctgctgaaga atgagaaata
      481 tctctttcat gccataatgt gacattttca taggcagtga ccatgtgtcc gcgcaccact
      541 ctggctagac cagccatatt ttctgaaccg atcggattcc ttttgtgcgg catagcagaa
      601 gaaccttttt gacctttcgc aaaaaattct tctacttcac gtgtttcgga tttttgcaat
      661 ccgcgtatct ctgttgcaaa acgttcaata cttgtagcaa taagcgccat cgctgagaaa
      721 tattcagcat gtagatcccg agggagaact tgagtagaaa tttcttgtgg acggatcccc
      781 aattgtttac atacatactc ttcaataaat ggcgggatat ttgcgaacgt tcccactgct
      841 cctgagattt ttcctgcttc tactcctttt gccgcatgtt caaatcgttc gatgttccgt
      901 ttcatttctg aataccatgt ggcaagtttc aatccaaaag tagttggttc agcatggaca
      961 ccgtgtgtcc gacccatcat cactgtatat ttgtgttctt ttgctttttc gccaatgatt
     1021 tccgtaaaac gtttcaagtc ttcccgtaaa atatcatttg cttgcttgat gagatagcca
     1081 taagcagtat ctacgacatc cgtacttgtt agaccataat ggacccattt tctttcttct
     1141 cccaatgttt cagaaaccgc tcgtgtaaac gcaacgacat catggcgtgt taatttttcg
     1201 atttccaaga tccgatcgat atcaaatgat gcatgctcac gaattttttt cacatcttct
     1261 tgcgggattt caccaagttc tgcccaagct tcatctgcta aaatctcgac ttcgagccac
     1321 gcacggtatt tgttctcttc cgtccagata ttccccattt cagggcgtgt ataacgttct
     1381 aacatgtttt ttctcctcta atcccaaata ttcgtttgtt tgatttcttc cagcgtttgc
     1441 tggatgtctt ctgtcaagat agtgatatgt cccatcttgc gacctttttt tgcttgtttt
     1501 tttccataga aatggaattg ccagtctggc ttattctcaa tcaagcgata gctttccagc
     1561 agttgttctc ccaaaacatt gaccattact gcattgctta tcaaacgtac tttttctcct
     1621 aatggccaac cacaaatacc tcgaatatgg gcatcgaact gactcataga acaagcttca
     1681 atagaataat gtccagaatt gtgaggacga ggagctaatt cattcacata tagcccgcct
     1741 gtttttgtta aaaacatttc tacccctaaa acacctgaaa ggctcactgc ttctgcaatc
     1801 acacgggcaa tacgctcagc ctcctcgatc acatcctgat caatcgcggc tggagcaatc
     1861 gtttcatgaa gaatattgtt gtggtggatg ttttccacga ccggaaaagt cgtgaaatct
     1921 ccttgaccat ttcctgccac catgatcgaa atctcttttt caaacgggat ccatgcttct
     1981 aaaacacagg ttccttctct taaaaggtcc atggcaggag ctagatccgc ccgacttttc
     2041 aaaacatact gccccttgcc atcatagccg cctcgagtgg ttttcaaaac acaaggatag
     2101 ccgatcccgt caatcgcatc ttggatatcc gtcggactga cgatcgttgc ataaggagca
     2161 atcacgatgt tattcgtttc caaaaaagat ttttctaaca ggcggtcttg cgtaatcgca
     2221 agcaagtctg tcccttgagg aataaatgac atgggtaaaa tcgcatttaa cgcctctaca
     2281 ctgacgtttt cgaattcata ggtaatgaca tctgttcttc tggcaagctc tttcagcgca
     2341 aaggtgtcat catagacact ttcaatatgc caatctgcaa cttgtgcggc tggacaatcg
     2401 ctcacaggat ccagcacacc tactttaaag cccatttctt ttgcactaat cgccatcata
     2461 cgacctaatt gaccgccacc gacgatcccg atcgttgaac caggcaacaa aggtttaacc
     2521 aagttgatca ctactttcta taacagtttt agccaacatt gaacgtcttt cagccaattt
     2581 agcctccaat tctaaatcat acatggaaag catctgtatc gcaagtagtc cagcattaat
     2641 tgctcccgct tttccgattg ctgtagtagc tacaggtact cctccaggca tttggacaat
     2701 cgaaagaagc gaatccaaac catttaatgt tcgtgattgg accggtacac cgataaccgg
     2761 caaagttgtt tttgatgcga ccatacccgg cagatgcgcg gctccccctg ctcctgcaat
     2821 aatgattttg attccttttt ttcgagcgtt ttgtgcatat tcaaacatca aatctggggt
     2881 tcgatgagca gaaacgacct ttttttcata agacacaccg aattcatcca atatttgaca
     2941 tgcttctttc attgtttccc aatctgaagt actccccata atcacagaga tttttgctgt
     3001 cattgttttt cctcccagtt tttctacatt catattttaa caatcacttt ttttgatgtc
     3061 aaagaaattt cgaatgttaa caaagaaaaa cattttattg ttcgtgttta ttaaagataa
     3121 aaaaaaggaa tgtgacaaaa gttaggtcac attccttttt atggataaac ggtgttctcc
     3181 tgccaactcc tcggtcacaa ggaacaatat tcaacaaaca tgagaagctt ttttttgaaa
     3241 ttgttccttg tgactcggag atgaacggca ggctcacgac ctcttccttc aacggtgttc
     3301 aatcagctga ccctcggtat tagctcaaac aaacggcaaa atacgaggaa ctatttttcc
     3361 gtttgtttct ctaacaatca ggtcaaaacg ctgatttcac aacctttctt ttgctctatt
     3421 acttcttgaa ggcttcttcc aatatttctt catctttttt caatccatta aagatcaaat
     3481 tcaatatgat agccgtaatg ctactcatta cgatgccatt tccagtgaac atttgcaatg
     3541 tttgcggcat ttgactgaat aatgtcggcg taacattgaa acccagccca aatccaacag
     3601 aaacagcaac aatcaataga tttttatcat tgctgaaatc aacttccaac agcatacgga
     3661 ttccttggac tgccaccatc ccgaacatga ctaacattcc tccaccaagt accggctcag
     3721 ggatgatttg tgctaacgca ccgattttag ggaataatcc taaaacaatc aagaagaatg
     3781 cagagaaata gatgggtttt cttgttttga ttcccgaaag ttgaaccaat ccgacatttt
     3841 gagaaaagcc agtataaggg aatgtattga aaataccgcc taagataacg gcaagtcctt
     3901 ctgcacgata tccctttttc aattcttctt ctccaacatt tttcccagta atgtcaccaa
     3961 gtgcaaaata tacacctgtt gattctacca tgctgaccat ggaaatgatg atcattagta
     4021 aaatcgacca tacgtcgaaa gttggtttac caaaataaaa gggttgtgga acatggaaca
     4081 acggcgcatc tccaatcgcg ctcagatcaa tcattcccat gaaagcagct aagatgcttc
     4141 ctccgactaa accaatcaat accgcgattg aacgaatgaa cccttttgcc agcatttgca
     4201 cgcctacaat taggagaatc gtcacaaatg caagtagcag atttgttttg tcaccaaaat
     4261 cagctgctgt tgcattacct ccacccattt tctcgattgc tacaggaatc aaagtcaaac
     4321 caataacggt gataaccgtt cctgttacta taggagggaa tagtttttta attttagaaa
     4381 agacaccaga aactaacaca acaaagatcc cagaagcgat aatagatccg tagatagctc
     4441 ctactccttc attggttccg atcaagatca gtggagctac tgcttggatc gcacatccta
     4501 aaacgacagg caagccaatt ccgaaaaatc ggttaactgt caattgcaat aatgtcgcta
     4561 atccacacat aaagatatca attgaaatca aataagtcat ctgttcttga ttaaagccta
     4621 gtcctgtacc gatcaaaaga ggcaccgcta ctgctcctgc atacatcgct agcagatgct
     4681 gcagccctaa aaccgcagct tttccatttt gtgtttcttt ttccacgatt atgcgtcctc
     4741 ctcaagaaac tcaacttgtc cattcgtcaa ggaagcaata cgcgcaagag aaacgactct
     4801 caatcccatt tcttcaagta actgacgtcc atcttggaaa gacttttcga tgacgatccc
     4861 gattccttct acttgcgctc cagcttgctg gcataattcg atcaatcctt ttgccgcttg
     4921 tccgtttgcc aagaaatcgt cgataatcaa tactttgtct tctgaagata aaaatttacg
     4981 agaaatagaa atcgtacttg tcacttgttt cgtaaaagaa taaacagaag ctgtcaataa
     5041 ttcttcatcc atcgtcaaac tttttgcttt tctagcgaag atcattggaa catctaattc
     5101 tcttgcagca aataaagctg gtgcgatccc agaagcttca atggtcacaa ctttcgtgat
     5161 tccttgttct ttaaatactt ctgcaaaccg ttttcccatt tgttccatca atacagggtc
     5221 tacttggtga gtgacgaagc tgtcgacttt taatacgccc tcgcctaata tacgtccgtc
     5281 tttttgaata cgttcaacta attctttcac tttatttgct cctttatcat ttattaatac
     5341 cgaaataacc gcttttgtgc aaaaacctac tatgtttctg ttaaagaaaa acaccctcca
     5401 ctttatcata agaaaagaag ggtgaaatag ccaatctttt acttatagtc cagcatttac
     5461 ggtactggct agaaactgtc atccatatta tgacgatata taagtaggta tgtaaaacta
     5521 ataatagtgt aaattcattt tcttgcaact gttttgcacg tgaaggcagt ttgccatatt
     5581 tgaataaaaa atgttgtttc aaatattgaa aacagtctaa cactactttc tgaaactgac
     5641 aacgtggttt acttattttt ttgtaaaatc taatgatttt taatgcatta ttcctttttg
     5701 ttcgggttta aatagttatt tttattttct cctatctaaa aaagcagccc acataaaaag
     5761 aaggcatgac aaaaagcatg tcatagcccc tttttccgaa taaatagtgt tcaaaagaca
     5821 gcctatcggc aataaggcaa gattttataa actacgggaa gtaatttccg taaatctatt
     5881 cttattcttt taagctgaca agctcgtcaa tcactactgc ttttcctgaa gcaatagaat
     5941 gatttgctgc gattcctgtc aaaatcgaca aaataccatc tacgtgagtt gctgcccgat
     6001 tccacggatc ttcttctttt tcttcaccaa atagatcatc taataaacgc ggatctcctc
     6061 cgccatgtcc gcccgcttct tgttcgacga ttacctcata tggctcaccg aacatcggat
     6121 agacatggat ggactgaaga acggctgctc cttcatcctt tttatctcca cctgcattga
     6181 tgtaagactt ttcgatcact ttcatttcga tccgtccttt gctgccattg aaagaaacat
     6241 tgaatccttc ccacggcata tacgcgttta gcgaatacgt caaaatcgca ccagtttcat
     6301 atttgaccgt tacgcctagt gtatcttcaa tagagatatc atcggaaaat acactgcggt
     6361 cacgaatata tccactgtct ttttctgctt ctaaatacat ttctcgcaat tcctcattgg
     6421 ctgacaggtc tatagcaaat ggatcagctt tagctagttc gcttccatag catcgactgt
     6481 aaaatatttc tactccgcgt ttctccgcat tatgcatccc ataaaagttt aatccaccga
     6541 atgcaaatac cttttgggga cggctgttca accaaaaatt cactagatca aaatgatggg
     6601 tagatttatg aacaagaagc ccgccgctat tgtgcttatt tcgatgccat cttctgaaat
     6661 aatctgcccc gtgttgcgta tttaacagcc attcgaagtg gatagaaaac acatcaccaa
     6721 tcacatcact ttctatcagt tcctttatct tcgtgtgata cggtgcataa cgataattaa
     6781 aagtgactcg caggcttctt cccgtctgtt tttggaccgt tattatttct tgagcttttt
     6841 cctcatcgat agtcagaggt ttttcgcaaa taacatcata tccttttttc attgcacgaa
     6901 tgatataatc atgatgcgtt cgatctactg acgtaataat gacttcagcc ggccgtacgt
     6961 catccagtag ttgctcaaat tgtgatggca tacatgtctt tacttttttc tgtccatact
     7021 tgttcacaag tatccgattt gcataatcca tccttgtttg agatacatca caaaaaccga
     7081 ctagttcaca agtttgatga tacgtagagg caattgcctc ataaaaaaaa cgcgctctcc
     7141 caccaagtcc gacttgtaca tattttttct tcatttaaag tcattctcct tttcttttaa
     7201 gcaaaatact gtgacaaatg gcagccctgg atacagtaat gcagtgagta aaaaatcatg
     7261 ctgtatacac cagaacagtc catacaaaag gcataaatac cacagaaacc ccttgacgtt
     7321 ttttgacaac agccagatat tccttttgat caaataaaca agactatctg ctttatcttc
     7381 tttgcagata tcaattagcg tactaaaaaa actatgggat acaaaaagca acagacacaa
     7441 tagaaaagga atcagatacc aagctccaat ctttcccact aataaatacc aatcaactgc
     7501 aaaaataatg acaaacagct gaaacaacgt aatgatgaaa cacatccgtt tgatttttcc
     7561 tttcctccaa catacctttt tttctgccaa gtaatggaca gatagataat aagaaggata
     7621 catcatcaaa aaataaatca gatagacaac acgagcagac agcactaatc ctaaagtgaa
     7681 caaacatcct agaactaaag cgaaaagagc attactgata agactggtta aaaaaatccg
     7741 acagacatag tagttgagct tgtaaagaaa catctcttct attggctttt tcatattcag
     7801 cccttcagac cgcttgtact gatcccctgg acaagatatt tattgaagaa taagaaaatc
     7861 agaaaaaccg gtagcagcga caacgtagac atcgcaaaca tactcgcgta agtcgtacta
     7921 gatccagcgt ctgcaaataa tttgatggca atcgataccg tgtaagatct cggcttgttt
     7981 aaataaatca atggtcccat gaagtcgtcc catttccaat aaaactggat gatgatcgtc
     8041 gtgatgatcg aaggtttcag caaaggaaaa ataattttgg taaagacact atacttgctg
     8101 catccgtcaa taatcgctga ttcatctaac tctttgggga tgctgaccat gaattgcatg
     8161 atctggtaaa tgaaaaatgc ttgtccaaaa aaatgtggta aaatcagcgg cacatacgtc
     8221 ccaaccaaat tcagccgatt gtaaataata aactgcggaa tcaaaatcac ttgtgcagga
     8281 atcatcatcg ttactaacat gacgacgaac cagaatcttt ttcctttgaa atcgattctc
     8341 gataatgcgt aagcaataca agtcgagctc agaactgtcc ctatcgtcga aactgcagag
     8401 atgacaaacg aattcctaaa gaaagtaaaa aatgaaattc cagcaaaccc cgcccatcct
     8461 tttgcgtaat tttcccattg aaaagtctct ggaatcaaat tcagcgaacc actcataatc
     8521 tcttgatccg ttttaaaaga acctgcgatc atccataaga ccggataaat catgactaga
     8581 cctaatagca aaataaaacc gtattgaaac catttcgtta ctgtattatt catttcttcc
     8641 tcctctcatt cacacaacga cccatttttt tgatgatttg aaaatcagaa aggtgacagc
     8701 tcccataatc aacagcatga tccacgaaat agcactagca taccccatag aattaaaaga
     8761 aaaagcttgg ttatacacat aaagagcata aaagttcgtg gcatcattcg gaccaccttg
     8821 ggtaatgata aatgcctgcg taaaagtcat aaacgccgcg attgtctgca taaccaagtt
     8881 atacaaaatg atcggcgaaa gaagaggtaa cgtgatcttg taaaacgtct gccatttgtt
     8941 cgctccatcg atctctgccg cttcataata cgagcctggg atctctttga tacctgcagc
     9001 aaagatgatc attgccgaac cgaattgcca agcagacatc aaaacaagag ggatcatgac
     9061 cagtttttga tcgctaaacc aggaaagcgc aggtgcgcct aatcgttcca ggaaaatatt
     9121 cactaacccg tttttagaaa acaactcccg ccaaactaga gcgatagaaa tactgccacc
     9181 gatcaaacta ggcacataat acaaagaacg aaagaaagtc gttcctttga tattcctcgt
     9241 taaaagataa gcaatcaata gaccaaagat cagtttcaca ggtacagatg caaatacata
     9301 gagaaaagtg actcggatgg agttcaaaaa acgggcatca ctcagcatct ttatataatt
     9361 atccaagccg atccatgaga ctggatttcc caaacgataa tcggtgaacg aataataaag
     9421 tgacaagatc atcggtatca ctgttatcaa tagaaatcct aggatgaatg gagaagcaaa
     9481 tacgtaaccg accacattgt cttgatccaa gaaccgtttt agtttttctt ttttcatgcc
     9541 ttcctcctgc tgctatccta gaatgtttga cgcaaactca taaagactct ctgctgcttc
     9601 ttctgctgtt acttcttcat aaatgacttt ttcaactaaa agattgtatt ggtcttgaat
     9661 ttccggattc aaagcactat caaagacatt cacttcgccg ttgtccatat cggaaatcat
     9721 ttctaaataa tcaaatgtcg cttgcaaagt tgcatcatct gcacgagcga gctgttctcg
     9781 aatattcgac atgattggta caccacgttc tccttttaaa atttcattgg cttcttcact
     9841 gttttgaaag aaatcaataa acttagcagc ttcttcttta tgggctgagc tttttgccat
     9901 actcagcatt tgcgaagaac gtaaagggat accagattct ccatctgctt ttctgcgagg
     9961 tggattaatc aactgaatct ccatatcttc aggagcccct gacaatattt ctgtcaactg
    10021 gttacttgat agaaacacca ttgccgcttc tctatcccta accgcctgaa aagacagatc
    10081 cgtcgtactt gcagctccac cacctggttc tggatgggcc cctttgtcta ccagattttt
    10141 acgcagctgg aaaaaatctg ttagatagtc tggattttca aatcccaagc tgttagatag
    10201 atcttcccga ttataaaaat tcaatccttt ctcaacttgt ggtatttgaa taatacagcc
    10261 agctataaaa tcttcgaatc ttgaaatagc atagattcct aattcttcag tgatttgata
    10321 gctgatttgt tcaaattcat cccatgtcca atttgaagct ggttcaggga tatttaattg
    10381 ttcaaatata tttttattgt aagcaatcgc aaacgaattc accccgttag acagtccaac
    10441 taattgcccg tcgtaagtcg tcgttgctaa ataggattca tctgcattag aaatatcaat
    10501 tatcccttgc tccacatatt catctaatgg ttcaatctga ttgatgtatt gaggaaattg
    10561 attgttcagt tggaaaatgt cccagatatc atctgatgct gctagtgtac ctaattttgt
    10621 gtaatatcct tcataatcat aaaattcata atcgatcgtc acatggggat ttttttcttc
    10681 ataaagctcg attactttta ctgttgcatc gtgtcggact tgcgaacccc accaagccat
    10741 gttcagcgtg atttttttgt catcttcttg cccacaagcc gtcaagaaga ataacataac
    10801 acttaaagct aagatgattc tcattctttt cttcattctc tgcacctcta cttccattgt
    10861 tatttttcaa tatactgtat ttttatacag aacatattta ttaaacgatc tttttcaagg
    10921 ggaataaaaa ccaagacttg ccttatcgtt ctgcctagga tgaacaactc aatactttca
    10981 agaaaaatta gatagtgctg attcttcttt catcgctaaa aacacttctt gcttcaatga
    11041 tgggaggata attacgcaaa gaaaaaagga tatcagacaa ctgcccaata tccttgacat
    11101 agctaaataa gcatgcttct tcgtgtgcgc tcttccctaa aaaacagaaa aataactagt
    11161 aattctgtat gaaattaggt acaatataca cgtcgatgca caagcattga ctatggcaac
    11221 ttgattggtc agttcccgct gacttttcaa gccccgacaa tttagctgct actaaattgt
    11281 cgggtgcctt ttttagtttt tattcgtttt tcattctata gaaagcgctt caataaatca
    11341 agacattttt ttgttagaat gctaataata acaaaaggtt ttaactcttc ttatgtacat
    11401 tacaggtaaa aaaacaaaaa aatttctctt ttttttgaat tctgaacaaa tagtactgga
    11461 gtacaaaaga cgaggaagag ccactatctg ctcttcctcg tcttttgatt ctcacctgtt
    11521 attcatgatt ctttttctca gtttcccgat atttttccat tttacgacta aaaagctctc
    11581 cgctacttgg tggcgtatat gtgatggcat ttgctcctgc ttgaatggtc tcagcaatac
    11641 tttcttccgt tggaccacct gtcgcaatga taggaagttc aggatatttt ttacgaaaat
    11701 ggcgtactgc ttctgcagtt tctttacctg cactgatatt gataatcgaa ataccagctg
    11761 ctaacttttc atctatttct gtatatttcg acgttaccgt actgataact ggaatatcaa
    11821 ctactcgaca aacttctttt actgtctcta aaggcgtagg accgttcaaa acgaccccta
    11881 acgaaccttg cgcttctgcg aacatgctca tgtaggaaga acgagcacct tgtgtcaaac
    11941 caccacctac accggagaaa actggaatgt cagctgcctc aataatgctt ttagtaatcg
    12001 ctggatgagg tgtaaaagga tacacagcaa ttactgcatt cgcatttgta ttacgtatga
    12061 tcgcaatatc cgtagtaaag atgatcgaac ggatcaattt tccaaatatc ttgatcccgc
    12121 ttgcttctaa aatgatttcc ggtactctta cgatatcttt tctcaattcc gtcatgattt
    12181 ctggcgtaaa ttgttctgcc atcagccact tctcctttgc tatctccggt catactgatt
    12241 caattgatac gtctcgatga cctggatgat tttttgcgca taaccggggt ctgtcgcata
    12301 acctgcatct tgcaatgctt gtgcggcttc ctgatagttg gtggccgtaa tcactttttc
    12361 atacagcgca ggattccaat ctactccttg gacgaacagt tgtgtatggt catccatgga
    12421 ctgttcccat gaatcataca ctttgaagtc tccttggata gttatccatt cttcgttaac
    12481 aaattccttt gtttccaggc ttactttctt ctgatcccca taagccttga tcccgaaaag
    12541 attattgtat ttgctagcca aggtactttt tccccaattt gattctaata tagcttgtcc
    12601 taaaatgata ctaggaagca ctccataacc atcttgtagt tcttgggcat gcggaacaag
    12661 ccgttcaata aattgctgat gggattgcgc ttcattttct tgctgattat cagtaaaagg
    12721 agaagacaga ttcttcaagg aaaaaacaaa ggctaagagt atcactgcta caccggcaac
    12781 cactgctggc caacggatat tcttgcgctt ttgctttttc cacttttttt tcgccatgat
    12841 caattcctac ttttcattta attcattcag atattcttct agcgtcaatt ggtgtttggt
    12901 gatatactca gcgttttctt taccgacata acgtaagtgc caaggttcat atgtgatctt
    12961 agttatatct tcgtgtcctt ttggataacg aacgataaat ccatactgcg cagcattatc
    13021 tgcgatccat ttagctccag gctgatctcc gtaacttgca tccaatacag tagaaggata
    13081 actgttgtac cagttttgat cgacgacatc gactgccaga cctgtgtggt gttcgctgta
    13141 tcctggctcc gtaatcgttt ctttagtaat ctttgtcgcc tcctcttctg atacaccttg
    13201 acgactcatc acatcttgga cattttgttc gaaaacttgt tgttgggatg atacagaccg
    13261 gtaagctgag accatcacta atggaaatcc agctgcttca gcagccttgg caaatgcttc
    13321 atattctgga gcaatgcgtt tgtcgatcaa gtatccgtta cttaatgatg ccaattgagt
    13381 agactcatca atttcttttt ctaatttgtg atctggacca accaaaacta gcttccaatc
    13441 gtctgtttta acatctggca attgtttgga cgtacgatct tttgtttctt tgtcctttac
    13501 cttgctgctg gttgtttgac tgttcttttc tttactagca gttgttttgg tgcttgttcg
    13561 ttcgactgct tgattgccgg cttgaacatg cgaatcatca tcgctaaaaa taacatagcc
    13621 cattgctgcg atcataatca caactgctcc aaatcctaaa aatttattca cttgaaaaat
    13681 cctttcgttg ttcttcacgt tccccattcg cctcatgaac gtaagtttct tccagatttt
    13741 cattcaccca atcgagaagt tcgatttttt ctccttcgat ctgtttttga acatctgctg
    13801 gcagacgaaa attcaaaata tcatcatagc cccattcatc atattgaata cttactttca
    13861 agtcaaggta tctaccagcc tgctgattat ttttcaaagg gatcagctcg acttttgctg
    13921 ctttgctgct aagccatttt tgcgcgatgc gtgttttgat ttgcttgtac tctgcaacgg
    13981 caacgtttcg tttttcggga aaaataatcg tttgacgcaa aatcttttgc gttagcatcc
    14041 attcataatc agtgaaaaga ttttttactt tctgcatcgc ttcagatggc agatttttct
    14101 cgccatcttt ccatttttgc cattgttctg gactgatcag caagtattca tctaaaaaat
    14161 ttttttcagt ttcaaactgt tctaataata cactcataat aagatcaata tatacttgat
    14221 gcacgtttat cccctccttt taactattct acttaagttt tgatcaaaaa gcacctctta
    14281 aatcgctgct ttcatttttt ttaatgtttc aagtcctctc ctctttattc ttcgtgattt
    14341 ttgttataat aatagacaga caagaaactc aatccaacta tgaaggagcg ttttcagatg
    14401 aaaaaatatc aatggggaat cgttggtctt ggtaccatcg cacatgagtt tgccgaaagc
    14461 ttcaatcaag agacgagcga actaacagca gttgcttcca gaacttcaga aaaagcggaa
    14521 aacttcgctc accgttacaa tattccaaaa gcttacggaa gctatcaaga aatgttagac
    14581 gatgctgaaa tcgacatcgt gtacattgct gtaccaaata gacagcatat cgaccatatc
    14641 ttggcagcat tggaagcagg caaacacgtt ctatgtgaaa aagccatcac gatgaataaa
    14701 aaagaactgg ctgacgctat gcgcttagca gaagaaaaga atttgatctt agcagaagcg
    14761 atgacaatct tcaacatgcc actttatcag cagttacgct caataatgga tacaggaaaa
    14821 ctgggggctt taaaaatgat ccaagcacct ttcggcagtt acaaagaacc agaccctaaa
    14881 aaccggttct tcaatccaga attagctgga ggagctctgt tggatatcgg tacgtatgct
    14941 gtatctttcg ctcgtttctt tttgagctct caacctgaag tagttgcctc tacaatggtt
    15001 ccttttgaaa caggcgtgga cgaacaatcc gtcactatct tgcgtaacaa agaaaatgaa
    15061 cttgctgctg tcagcttgac ttttcaggca aaaatgccaa aagtcggtat cgttgccttt
    15121 gagaacggtt atgtgacgat tgctgattac ccgcgtgctg atcgtgcgga gatcttattt
    15181 acagatggta caaaggaatt catcgaaagc gggaatacat ctgaagcaat gaattacgaa
    15241 ataaagaata tggtacacat gattgaaggt gacttgccca atcgctctct cttcttgaca
    15301 aaagatgtca tcgaaatctt ggatcaaatg caagttctat ggaaaaatat gtaattttaa
    15361 aaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa aaatagctcc
    15421 tcctattttt cgtttggctg gacttattac cgcagaggaa agcttttgaa caccgtttaa
    15481 gaaagaggtt gtgagcctac cgttcagctc cgagtcacaa ggagcaattt caaaaaacag
    15541 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttgacagga gaacaccgtt
    15601 taaagaagag gttgtgaaat cagctttttg acctgaatat tagctgaatt cacaacctct
    15661 atttttggat tttttgttgg gagaattctg ccttgatttc aataaaactt ttaaaagact
    15721 gcaaaaactg gaacaattct gctcgatcct cgaattcagg gcgactagtc ggcagaggca
    15781 tttgccgata caatacataa acttcttcaa tacgtgttaa aatttgtctg ccatcatttg
    15841 actctgaaaa tgtttctgca gtataaatca ataaaccata gatatgtttt ccataataag
    15901 ctggttgctg gattctctct agattttcca gcatgtcttt aatcacattg gcctgtgctc
    15961 ttctcataga gaagtaagtt tcgtagtata gcggctgtct gagccagtag ttttcttgat
    16021 gttctctggc atcttcttgg ctttcgcgaa tgaatgtcag taaatgttcg catttgatct
    16081 gcacatcttc tttattttct gataaaagaa attctgccat ttgtctcaaa atatttcgaa
    16141 attctttttc gatctgtatt tggttttcct tcagctgctt cgtacgatct ggcatgtaaa
    16201 catttgcaag caaagcaaat actaatccaa tcgccattaa tcccgcttca ttgccgatca
    16261 gctgccaaga catgttttcc tccactaaat aatgagtgac caaaaccgaa tttactacta
    16321 tcccttcggt taatttgaag cgagaggcaa gcgggatgaa caggaataaa aaaagactga
    16381 agccaaaaat agtataccca acagcaaaaa aactgaacag tgctgttgcc gtagctaata
    16441 taaaggctgc gattcgattc atgcccgtac gcaatgtaga gcgcttggta tttcctacac
    16501 ttaataatgc aatgatccct gcagctggcc aatacaacag cgataagcta cccgctgtga
    16561 ccatcgaaaa gaagacactg atcactgttt tgactgttcg cattcctata cgcatttact
    16621 tccccctttt ttattttaat tatacttgaa taatgaaaaa ctagctatca gacaaaagtt
    16681 taggtatgat agtaaaaaga ccaaaaagga gaatctctat gcaatttggc cgttttcgac
    16741 taggtatgcg tacgatcaaa agtgccttag ctgttttttt atgtatcttg ttttttcatt
    16801 tgactcaacg aggaatcccg atgattgctg cattatctgc tgttttttct ttgcgtcagg
    16861 acttgactac aaccgtttct tttggaaagt ctaggatcat cggcaataca ttaggtggta
    16921 cattagctat catttatttt tttgtcaaaa gctattttca caatgatttt ttggtagaat
    16981 tgatcttgct tcctattcta gttattattg tgatcgtcgt gtcagatggt attaacaata
    17041 attccggtat catttccgca atagctacgt tgttgctgat tgcgttgagc atccctcaag
    17101 gcgaatccgc tttgtatgct atccaacgtg tactagatac ttttataggg acattcattg
    17161 caatcggtat caatttcttt cttcgtcctc ctgaaacaga aaagaaggaa gaactatcag
    17221 aagatttggt cgaactccaa aagaaagaac gattgctaag aacaaattta caagagatag
    17281 aagctaagat ccaaaaacaa aagaaaaata gcgaggacta attttctttt gcaataaaat
    17341 aaaaaaatgg agagaagaac tttaaaaagt cttcctccat ttttgattga acggtgaagt
    17401 gtactacttt attcttcctt catcgagttt tgttctgcct cttttggcgc ttcatacata
    17461 tttacttggc tagtagtccc acgtgtcgac aaattctcta cgatttcctt caagtcaagt
    17521 cctgttgtcg cttttagtgt ttcttgagta gaagaaagca aatttgtggc ataatttgtc
    17581 actcggttcg ctccactggt ttctcctgaa ctgcttcctg tatcgacaac agagattttt
    17641 tcgatatttc ctagcggctg tgctgcttcg cgcatcaatt gcggtagcat atcgataacc
    17701 atactcaaga cagctgcttc gccatattcc ttgaaggcat tggcgatctt ttgttttgct
    17761 tcggcttctg cttttccttt tgctagtgct gcttcggctt ctgcttgtcc agctaaacgt
    17821 gtcttattgg cttccgcttc tgccagtgct tccacacgga atcgttctgc ttcggcttca
    17881 gtcacttctt tgactttttg agcttgtgct tcttgttctt tagcgtaccg atcggcatct
    17941 gcttttttct tcacttccga atcatattgc ttttcacgac gtgtgatttc tttttcttcc
    18001 agttcgattt gtttttgacg ttctacgatt ttaatctgca tttcctgttc gattacttgt
    18061 tgttgtgctc gggcactttc taaattgtag gcttggtctg cttttgcttt agcaacatct
    18121 tgttcttgtt tgtaggttgc taatttcagt tctttttctt ttaatgattc tgcgatctct
    18181 gtttgtcgct gtaattcagc tgcttgtgat tctttttctg cttgagcttt tttgatacgc
    18241 gtttctttta gagcttccgc ttctgctata tctgcatctc ttttgacttg ggcgattctt
    18301 ggtttcccta aagagtcaag ataaccgttt ttgtctctga cctctttgat agtgaaagaa
    18361 acgatgatca accccatttt tgcaaggtct acagaagcga cttcttgtac gctttgactg
    18421 aacttatcgc gattttgata gatttcttct acagtcatag aaccaagaat cgagcgcaag
    18481 tgtccttcta atacttctct tgcttcattt tccagttctt ctcttgtctt gcctaaaaac
    18541 tgttctgctg ctgtagcaat ctcttctaca gatgaaccga ttttgatgat cgaagtacca
    18601 tcacacatca ccggtacgcc ttgttccgta tacacttcag gggtggagac gtccaattta
    18661 ctcgataata gactgatccg attcgaacgt tgaaaaactg gcaagacgaa tgcgccacct
    18721 ccacgcacaa ttttgatttt gttattgctt tcatcaatgt gcacattttt gcttcctaaa
    18781 taacttccac tgatgatcaa cgcttcatcc ggttttgctg tctggtactt cgctacaaag
    18841 acgatcagca acatcagtaa aatcaaagct gcgattgcga tcgcgataaa aacaactggt
    18901 atttccattt aacacacgct cctttaagct tttatcatgc tttcataagg catgacataa
    18961 cagattcttt ccttgatctc aatgatcaat actttcgttc cccgctcagc tgtctgttct
    19021 tgggggctgt aaaaacaagc tggacgacta atggaaccgg taacactttt caactgaatt
    19081 tctcccatac ctcgtacagg gatcggtgtg atcacaacag ctacttgtcc ttccagagtt
    19141 ttttcagaag acgacatcgt tgattcggca tgtttcatcg gcatcaatat atagaaatta
    19201 agcaaaaata ccaacactat cgatatgcct ccactagcaa taaataaaaa taaagatgat
    19261 tgatctgtta aacgctcacc tagaaaacca aataagcttg tgaaagttaa ccaagggata
    19321 ataagcatcg gatcaagcgg gccatcaaaa tcgaatacat ctccaaaaat aatcaatagg
    19381 actgccaatc ctgcacaaat cagcaatgta taccaataaa tcgtctcaag tgcgattccc
    19441 cctatcatat acacgcctcc ttccatttct acgtctatca taccaatgtt tgaaaattct
    19501 gtatagcttc ttttaccgac tcagtggtgt tttttatagg aaggttgtgt ttatcgttga
    19561 caatttgtta aatttttgtt ttcatgttaa aattttcaca ataaattctt tcgaggtagc
    19621 ctatgtttcc tttttttaag aaaaaaccat taaagctcca gaacacgatc atccttgtcg
    19681 tttgtttctc cacattcgtt tctttgattg tttctgccat attgatcagg aattttattc
    19741 tttcgatgaa ccgatcttgt gtgtcagata aaatctctag cattgctaaa atcgttgcca
    19801 atgataaact catccaaaaa aagctgcaga ctggtgaccc gacacatgcg attcaaggaa
    19861 taacaaatga aatcatgatt gcatcgaatt tagattttat cgttgtcatg gaccctgaag
    19921 gcattcgata ttctcacgca gacccaagtt acatcggtct tcctttctca agtcatgaag
    19981 atgtgaaaaa agtgattgga ggacaagaat ttttctctga aaaagaagga atgttagggt
    20041 tcggcttccg ctatttcgtc cctatttatt atgatcaaga gattattggt gcagtttgtg
    20101 ttggcttaac aaacaacacg attgacaatc aagtcaaaga tggacaaaaa atgattgttt
    20161 tttcattgat tattggactt agcgtaggca tcataggcgc cgtttttctt gcccaaaaaa
    20221 tgaaaaaagt actgcttgga cttgaaccac aagaaatagc agaacagtta agtgaaaaaa
    20281 cgattatcga aaatgaagta gatgaaggaa tcttagccat ctcttctgat aaaactatcc
    20341 tcttgatcaa tacacctgca aaaaacattt tctaccaaac agagcagcta ttggatttcg
    20401 aagtaggaaa aaaatcagtg ataatctata tgacgttttg tttaaagaga cgattgagca
    20461 tcaaacgacc gttcggactc aatccatgta tttaaatggg atagaaacta tttctagcgt
    20521 tacacctctt tttagcaatg accgtttttt aggagcagtc gcaactttac gaaatcgatc
    20581 agaagtaatg aaattaatgg aggagcttgg ggggacgaaa caatatataa actccatgag
    20641 agctcaaacc catgaattca tgaataaact tcatgtcatt tctggtttga ttgatttgaa
    20701 gaagtatgaa gaagtcaccc attttatcca gcagctaaat caaaattata aaaaagagat
    20761 tggccaagtc tcaagtctga tcaaagtacc tgcaattgcc ggttttttat taggcaaaat
    20821 aaacgaagcc aaagaacaat ctgttgaaat gctgttagaa agcgattctt ttatccctaa
    20881 aatggagatg gatgactcca tccattcact tttgcaagtg ttagggaact tacttgataa
    20941 tgcaaaagaa gccgtgctac aaaaacatga aagaaacgga caggtccgga ttcatttaaa
    21001 ctatgacaca gaagctgaag tattcatcgt gaccgtctgc gatgacggtg tcgggatatc
    21061 agaagaaatc tcagaaaaaa tatttacgct aggtttttcg acgaagggaa tcaatcgtgg
    21121 gtttggattg aatctcatcc attcaatcgt caaacgccac ggtggatttt tagaattttc
    21181 caaacaaaca aatggcggta caatcgtcta tctagaatta ccaaaaaagg aagatcaaaa
    21241 tgatgacaaa tgtcctgata atcgaagatg atccaatggt tgcagaattg aatcgtactt
    21301 ttgtggaaag catccctaat atgaacgtaa tcgaaagttt cagaaatgta gaagaaggaa
    21361 aaaaatattt cagacaaaaa aacgcagatc tgctgctgct tgatgtatac atgcctggcg
    21421 aaagtggtat ccatttttta tcttggctta gacagcaagg aaatgatatc ccagtgattt
    21481 tgatcactgc tgcagatgat atcgaaacca taaagaatgt gatccgctac ggtgccttag
    21541 attatttgat caaaccgttc actttgcaac gttttcagct ggcgatcgaa aagtatcttg
    21601 ctttctctca gacaatccaa acgactgaaa aagccgatca gaaattgatc gatcaagtat
    21661 tcaaccacac cactcccatt cacgaaatac catcattcat cgatttgcct aaaggtctgt
    21721 caaaactgac tttaaaaaaa ataagtgcat ttattctaaa agaagatact attttttcaa
    21781 caaaagaact ggctcaaaaa agctcactgt cacgtatatc cgtcaagaaa tatttgaatt
    21841 ttttagtaga acaagatata ttgaacgaaa gaatcaaata tttggaaatc ggccggccgc
    21901 taacctgtta tgaattgaca aagaatgggc ggaaaaagct cacagattac tgttgagtca
    21961 tcgaattatc ccatacaaaa tagtccaagt atacagaagt aacaactgtt tacttggact
    22021 atttttaaac attttattga cgaatatcca tgttttctta tcttaattga cgggctttag
    22081 gaagagatac ctcatttttc gtttttccag tttctattat ttcacgaaag ttctcgaaac
    22141 ttgtcgaaat catatttgat aatgcctcat ctgtatttga acctacatgc ggggttacaa
    22201 gaactctagg gtacatttct actaattgct ggatagcagg gtctggaatc gtctcccatg
    22261 gcttgaagga tcggaaaaaa acctctgttt catttgcaaa aacatcggtt gcaaaaccag
    22321 ctaaatgatt gcttttcaaa gctcttaaaa tagcttcatt atcttgaagc tctccacggg
    22381 ctgtattaat caagatgcta ccttttttca tttgagctaa aaatccttca ttgatcattt
    22441 gatcgttttc acctgggata tgaggtacat gaatactgac aatatcgctt ttggctaata
    22501 actcttcaag cgagtggaac gtcaatactt ttttagcagc ttctgtctga tgaatatcgt
    22561 agccgatcac tttggctcct agtcccttaa acaaaacagc ttccgtcaac cctatcctgc
    22621 ctgttcctat gatccctact gtgcaatttc tgatttcacg actaaacatt tgctcatcca
    22681 ctcgaaaatc tttgaaagat gttcggatcg tcgtatacgc agtatttctc aaaagcatca
    22741 tggcaaaagt caaagataac tctgctatcg catttggcga atacgatggt acacgggcta
    22801 ctgtcatacc aaaatcagct gccgcttgta gatcgatatg attgactccc acagtccgtg
    22861 taaaaacata acgaatccca tattcctgca tttttgcaag attggtctca tctgctatac
    22921 aatttcccct caacaataca gcatcatggt cctttgctga cacgatattg tcatgtgtca
    22981 acagttcttc ataaagactg agatcataat ggtaaatatt caatttttca aaaaaatcta
    23041 tctcgttcgg acgaacaccg taacatgcga ttctcatact ttcttcctcc atttcttatg
    23101 aaaataaacc tatgccatga atcatttgta atacaacgat ccatacaggt gtcagtacca
    23161 cagcaaaaat agttgacagt aaggatgcat tggaggctaa aagtgcctcc ctatcaaaac
    23221 tgatagcata ggctgctgcc actgttgcag ctggtgtagc catcatgata acgattgttg
    23281 ccagtgcttc aaaattgact ggtagaatat tcgttgccgt caaaactgct agaatcacga
    23341 tattcaatgc aggtacaaat accactttga ctagactgta ataccatgaa gttttttcag
    23401 atgcagcttt tttgaagctt acttcaccta atgttgcgcc gattgctagc cacgccaatg
    23461 gtgaacttaa accagctaaa taatccatcg gctgaaatag ccacggtgca gtctggtcaa
    23521 ttcgcaaaaa cgctacttgg cttatctctc cttctgcacc tgtaactgac acttgtggca
    23581 gatacccttg gaacagccaa atgaaaaggc cggcaaaagt agcgataatg attggattga
    23641 cgaacatcgc tttgatattt ttagcttcca tcttcaagcc actcattttt atgtatccat
    23701 aagaatacaa aaagattcgg tagccaatat tgaaaattga ggaatacatc acgccgacag
    23761 gaccataaat cgcactgacg ataggcgttc cgaaaaaggt cgtagaacca aagatcgtca
    23821 ggacacgcaa cgttgtttgc ttatcccctt tatagcttaa gaaaaaaggt tttgaaacta
    23881 gaataagaat aatataaata actaatcccc aaatcaagac attgattcct tgcatcagta
    23941 attctgtatt gatatcttgc atgaaagctc gaaatgccag tgctggcaaa gccactgtta
    24001 agacgacttt cgatagaatt tttccaacag attcagaaaa aatccccttt tttctacaaa
    24061 caaaccctaa taatataata aaaactgttg aagtgattgc actgacaatc gacatatctg
    24121 tcaaagtcga tcgaaaaatg tcaaataagt tcatctttct cctcctaatt atgttcattt
    24181 attcacaata aaaaaagctt ttattttgct aatacgtatt aacattttta attttatct
//
LOCUS       DACCWK010000033        24055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCWK010000033 DACCWK010000000
VERSION     DACCWK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.033"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IVW65_001280"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IVW65_001280"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7900167.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IVW65_001281"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IVW65_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7900168.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IVW65_001282"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IVW65_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7900169.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IVW65_001283"
     CDS             complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IVW65_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7900170.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IVW65_001284"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IVW65_001284"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7900171.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IVW65_001285"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IVW65_001285"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7900172.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="IVW65_001286"
     CDS             complement(8557..9027)
                     /locus_tag="IVW65_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7900173.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="IVW65_001287"
     CDS             complement(9073..9498)
                     /locus_tag="IVW65_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7900174.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="IVW65_001288"
     CDS             complement(9565..9834)
                     /locus_tag="IVW65_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7900175.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="IVW65_001289"
     CDS             complement(9845..11440)
                     /locus_tag="IVW65_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7900176.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IVW65_001290"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IVW65_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7900177.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="IVW65_001291"
     CDS             complement(14993..15553)
                     /locus_tag="IVW65_001291"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7900178.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="IVW65_001292"
     CDS             15906..16880
                     /locus_tag="IVW65_001292"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7900179.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="IVW65_001293"
     CDS             17050..18450
                     /locus_tag="IVW65_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7900180.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="IVW65_001294"
     CDS             complement(18528..19250)
                     /locus_tag="IVW65_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7900181.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="IVW65_001295"
     CDS             complement(19329..19835)
                     /locus_tag="IVW65_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7900182.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="IVW65_001296"
     CDS             19958..21757
                     /locus_tag="IVW65_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7900183.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23399)
                     /locus_tag="IVW65_001297"
     CDS             complement(21825..23399)
                     /locus_tag="IVW65_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900184.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            23786..>24055
                     /locus_tag="IVW65_001298"
     CDS             23786..>24055
                     /locus_tag="IVW65_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7900185.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCG"
BASE COUNT         6603 a         5178 c         4371 g         7903 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga actattttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
    22441 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    22501 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22561 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22621 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22681 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22741 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22801 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22861 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22921 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22981 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    23041 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    23101 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    23161 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    23221 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    23281 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    23341 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    23401 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    23461 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    23521 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23581 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23641 ataaaggttc tataatattt tgtgttggtg gaaaatcgaa agcgattttc tactaacact
    23701 tttttcttcg tttcaactca aaaagagact cacttttggt aaaatttagt cgaccaaaac
    23761 gtcaccctac caaaggagtc tctcaatgga taatcatact agaaaattac tcggattaac
    23821 agataaacat ttattttttg atgaagaatg gctgattgaa aaagaatata aaggcgttca
    23881 agcgcagttt attaaaggga gattggatga ctcaccttcc cattgtgaac actgtggatc
    23941 gattcgtatt atccgcaacg gttcctatac gacacgcact cagatgttga aagtcaaaga
    24001 aaagttgact attttagaat taaaacgtac ccgttttctc tgccatgatt gtgga
//
LOCUS       DACCWK010000034        23588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCWK010000034 DACCWK010000000
VERSION     DACCWK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.034"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(120..653)
                     /locus_tag="IVW65_001299"
     CDS             complement(120..653)
                     /locus_tag="IVW65_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900186.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(950..3331)
                     /locus_tag="IVW65_001300"
     CDS             complement(950..3331)
                     /locus_tag="IVW65_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7900187.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3321..3968)
                     /gene="recU"
                     /locus_tag="IVW65_001301"
     CDS             complement(3321..3968)
                     /gene="recU"
                     /locus_tag="IVW65_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7900188.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            4032..4571
                     /locus_tag="IVW65_001302"
     CDS             4032..4571
                     /locus_tag="IVW65_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7900189.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4661..5080
                     /gene="gpsB"
                     /locus_tag="IVW65_001303"
     CDS             4661..5080
                     /gene="gpsB"
                     /locus_tag="IVW65_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7900190.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            5102..5469
                     /gene="rnpB"
                     /locus_tag="IVW65_001304"
     ncRNA           5102..5469
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVW65_001304"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5678..6844
                     /locus_tag="IVW65_001305"
     CDS             5678..6844
                     /locus_tag="IVW65_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7900191.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6890..8386
                     /locus_tag="IVW65_001306"
     CDS             6890..8386
                     /locus_tag="IVW65_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7900192.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8467..9150
                     /locus_tag="IVW65_001307"
     CDS             8467..9150
                     /locus_tag="IVW65_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7900193.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9795..10985
                     /locus_tag="IVW65_001308"
     CDS             9795..10985
                     /locus_tag="IVW65_001308"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7900194.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            11087..12040
                     /locus_tag="IVW65_001309"
     CDS             11087..12040
                     /locus_tag="IVW65_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7900195.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            12109..13458
                     /locus_tag="IVW65_001310"
     CDS             12109..13458
                     /locus_tag="IVW65_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7900196.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13641..13964)
                     /locus_tag="IVW65_001311"
     CDS             complement(13641..13964)
                     /locus_tag="IVW65_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900197.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            14093..15256
                     /locus_tag="IVW65_001312"
     CDS             14093..15256
                     /locus_tag="IVW65_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900198.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            15326..16675
                     /locus_tag="IVW65_001313"
     CDS             15326..16675
                     /locus_tag="IVW65_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7900199.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16720..17151)
                     /locus_tag="IVW65_001314"
     CDS             complement(16720..17151)
                     /locus_tag="IVW65_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7900200.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17539..20181
                     /gene="alaS"
                     /locus_tag="IVW65_001315"
     CDS             17539..20181
                     /gene="alaS"
                     /locus_tag="IVW65_001315"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7900201.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            20381..20977
                     /locus_tag="IVW65_001316"
     CDS             20381..20977
                     /locus_tag="IVW65_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7900202.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            21099..22247
                     /locus_tag="IVW65_001317"
     CDS             21099..22247
                     /locus_tag="IVW65_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7900203.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(22285..22698)
                     /locus_tag="IVW65_001318"
     CDS             complement(22285..22698)
                     /locus_tag="IVW65_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7900204.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22777..23208
                     /locus_tag="IVW65_001319"
     CDS             22777..23208
                     /locus_tag="IVW65_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7900205.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(23307..23540)
                     /locus_tag="IVW65_001320"
     CDS             complement(23307..23540)
                     /locus_tag="IVW65_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7900206.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         7651 a         4108 c         4893 g         6936 t
ORIGIN      
        1 tttctttaat tgcaaataaa tgtatgaatt atcttgttat tatggagaaa agctgtttcg
       61 tttatctttt ttgcatgaaa aagtaaatgt acatactcag tagctaggat ggtaacttat
      121 tacaatttat tgcgataaaa ataaaagttg ttatttttat tgggaaaata cccgatgtag
      181 aaaataatgc cttccaatat acctataaaa taagtccagt attccaaagg attatataaa
      241 taagtgacgt atctatctaa aggtgtaata aaaatgataa gtgataataa aaaaatacca
      301 tttataacta aacataaact aatggatttg ggcggtttca ggctcatcag aaaaatatag
      361 attctggtga acaacaacca taaaaaagtg agaatcattg catgagagct tctttccata
      421 aattttcctt taagagtagt gcttaaaagt acagaaaaaa taataaatgc aaggatagga
      481 gcaattaaag taaatttatt taataataaa caaaattttt cagctttttt attcttggaa
      541 taacgaggta ttgttttcga taaaaaaaga tggtgcagac aaattatgat aattaagcat
      601 actatcagat aactcaaaat gaagccttct ttcaaaaaat tattcaaaac catatattgt
      661 attttttatt tgataataac aatgggatta ttaagaagtg gcactaaata aaagactagt
      721 gtgtatttag ccgatttaat tataaacaag taaatggata aagtatatgt aatgataaaa
      781 gaaaaatgga ttttctaatt tttaagctaa atgttagatc acacatgtat atgctggata
      841 gtcttataaa aaatcatgta atacagcttg gtacatgatt tttatttgta gctgtttttt
      901 acatagacac aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac tatctgtttc
      961 cagaagctga ggtgctgctg gatggtgtat ggtcatttgc agcatccgct ccgctagttg
     1021 ctggctcact ggaagaagca ggaggagcag atgaagacgg tgtcgagctt tcttcggatg
     1081 aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt
     1141 ttgaggactc agcagttgat tcgctttgac tagtagagct agagctttga gtcgttgtct
     1201 ctgttgttga gcttccagga gtctggacat agctggaaga tggaatagtt gttgatggtg
     1261 taattgcatt cgatctagct gtatattgat ctttgtagta cagttcaccg cccacacgaa
     1321 ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca tactgcataa
     1381 gctcgcgata aacatcagag gctacgtgtg acgattctga agtgacaggt gtcatttttt
     1441 tattgtaacc tgtccacacg gaaatcgaat aatttggcgt atagcccgca aataggatat
     1501 ctggatacac cccagaagaa ataccaagtt ttgcatactc gtcatctgtg taattggatg
     1561 ttccggtctt accggcttga tataacccag cgatttgcgc attcgtacca gttccttcag
     1621 tgatcgtatc ttttaaaatg tctgtgatca tataagctgt ttctggagac atggctgttt
     1681 ttccatctgg ctcataagtt tcttctgttc cgtcttggaa gacgatttta ttaacatatt
     1741 gaggtttgta ataagtccct ccattggcga aagccgcata ggctgctgcc atttttaagg
     1801 aagaagctcc gtattttgtc ccatcttgtt cttccgtatt actagagatc gcattcgatt
     1861 gatggatcgt actgtattcg atcccaagat tttttaagaa agaagccact ttatctgaac
     1921 cgacttcatt gaataatttg actgctggaa cattacggga taagtaaagt gcttggcgta
     1981 aagtaatggt gcccatatat tgattatccc agtttcctac aggagtcgaa gtcccttcat
     2041 agttatacgg tgcgtcagta atagtttttc cagtagaata tttcaaatat tcaaaagctg
     2101 gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta ttcactgcta
     2161 agttgttgcc taaagttacg tcttctgcga tgtgacgtcc gccaatctgg gcttttactt
     2221 tcccggtatt ggtgtcgata agggtggagg caacttgcat ctcgtcgtct gggtaagaga
     2281 catactgatc cgtgttgacg atatcatata gtttcttttg ggcgtccaga tccaaattcg
     2341 tataaatctc taaaccgtct gtgtagacat tcttatctgt tttttcttgt acttcattga
     2401 tgacttcttt gacataatta tcgacgatct tagtattgtc atcggattga gttagttctt
     2461 gcagaccatc cgttaccggt acattaacag cttgatcata ttctgtttga gagatttttt
     2521 cgttttgaag catcgtatac aaaacagtat ctcgtctttt cttcgcctga tctggataaa
     2581 cataaggatc ataggcggaa ggggcttgag gcatcccagc aagcaatgct gtttgcggta
     2641 aagaaagttc tgataatttc ttaccaaagt acatctcaga agctgtctcc atcccgtaca
     2701 agccgtttga catatacact ttatttacgt agtaggtcaa gatctcttgt ttcgattttt
     2761 tttgttccaa tcttacagcc atccacgctt cttgggcctt gcgtttcaga gtctgatcct
     2821 cggcactggt ggagaagaag gaaagtttga tcaattgctg tgtcaaagta cttccgcctt
     2881 gcagtccgcc agaggtaaag ttagagaggg cagaaccgat gatccgaata gggtcaaccc
     2941 cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat cgcatcttct aatgttttcg
     3001 gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg aatagttcgc
     3061 catcttgtgt atatagtttg gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg
     3121 gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg atacagaaaa
     3181 atgagagtat tcctaaaagg atcttgataa ataatccttt tttatgtgtt ccagaggact
     3241 ttccagaacc tttgccagaa ttcttcttca cactcttttt aggagtaggc tgtttctgtc
     3301 gtctagagct tctagtttgt tcatttgcca ttcttgcttt ctccttcaat agataatgtt
     3361 gataaataga agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc agttttgatc
     3421 tctattccta gttcttcgat caaagataaa ggtagtgatt tgcgtccttt ttccttttgg
     3481 gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa aacagcgctt caaagacgaa
     3541 aaccataaca agacaaagca gaccccattt tgttcgaggc atgctttcat atgttcgatt
     3601 tgatgggcat ggaagttttt aaagggaaaa gatgttttgt tcttggtttc ttttgcttca
     3661 aagtccaagt aaaagcctcg atagacgcca ttatagtctg tagttgaggc ttgggtaaag
     3721 taggcttcct tgatcacagc agcacttctc ttaggatagt ctactttcac gatttggata
     3781 ggggtaggct ttttatggat aaccgccaac ccgcgggaaa gataatattt attactttca
     3841 ttgattgctt cttcaaagcg catcccccga ttgccgaatt cagtgatctg atttcttttt
     3901 tcttgaattt tttcgacggg agaggcattc ttataataag gttggccatt tggatagcgc
     3961 aataccatct ttaatcacac tcctaggcca cattatagca aaaaagcgga tagaaagaaa
     4021 aggaatctga gatggataag tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac
     4081 tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag
     4141 aactccatca gttagcagaa gaagggctgg aatgggtact tgtcagtggg aatttgggcg
     4201 tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga agaatatccg gaactatctc
     4261 ttggtatttt gtatcctttt tctgattttg gtaataactg gaatgaaggg aaccaagaaa
     4321 aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc tgttagccat caaccctacc
     4381 aatcacctag tcagctaaag aatcacactc gttttatact tgagcataca gtgggctgtc
     4441 tgctgatcta tgatgaagaa ttccctggaa aaacgaagta tttttttgaa gatgcacaga
     4501 attaccaagc agatcatccc tacgaaattc ggctgatcag tatggacgat cttcaaaatt
     4561 ttagtgaata atcgatattg tgtttgattt atcaactttt ttcctataca atgaaaaagg
     4621 tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat atggcgaatt tggtttatag
     4681 tcctaaagac attttacaac aagaattcaa aacaaaaatg cgcggctatg atcccgttga
     4741 agttgatgaa ttcttagaca atatcatcaa agattatgaa acttacagca aagaactcct
     4801 tgctttacaa gaagaaaacg atcgattgag tgcaaaagta gctcaattat ctaaaactca
     4861 aggagcagct caaacacgcg tgcaacaaac agaggcacca aagagcgcag cagtgaccaa
     4921 ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga
     4981 tcaacaagca agtgcagtca aaccagcaca acctaatcca aataactaca caaatgcaga
     5041 cacaagctta gatgataatg aaaaaacacg tcaattttaa tttttgatta atgaatttgc
     5101 ggttttcggg taatcgcggt ctgacttgat caggctgagg aaagtccatg ctcgcacagg
     5161 ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag ctaaggtact ctttggagta
     5221 acggcaggaa aacaagctaa ggcttcggct atgcttaagt atccttgaaa gtgccacagt
     5281 gacgaagcgg ctggggaaac ccatccggtg gaacgcggta aacccctcga gcgagcaacc
     5341 caaacaatgg taggggcgct ttttgtctgg aagaagaaca ggacaaaaag gcagtataac
     5401 tgcagataga tggttaccgc tttgtttttt ctccctggaa aagcaaagta cagaacatgg
     5461 cttatagaaa accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg cgttataaga
     5521 aacaagaaaa acatttgaaa gctatttttc aatacttcag atgaatcgtt cttattacaa
     5581 aacaaacttt tgtatcaccg gttattcgga atttgagtaa aagtgtgggg taggactgat
     5641 gtcccacctc ctttttttat aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa
     5701 cctcgtcgca actgcggcaa gcgggctaga agcactggtt ggtaaagaat taagagatct
     5761 tggaatcgaa tgccaagttg agaacggacg tgcacgtttt gaaggaacaa tggaaacgat
     5821 cgctacagct aatttatggt tacgtacagc agatcgagta aaaatcgtgg ttggggaatt
     5881 cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa gcattgcctt gggaagattt
     5941 tcttccacta gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga aactatatag
     6001 cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta
     6061 tcaccgtcca gcttctgtac cactgacgga aactggcgca cattttcagt tggaagtagc
     6121 tttgctgaaa gaccatgtga tggtaactct ggatacgaca ggaccaagtt tattcaaacg
     6181 tggttatcgc ttagaaaaag gtggagcacc attgaaagaa aacatggcag cagcacttat
     6241 tatgttgaca aattggcgaa aagaccgtcc attttatgat cctgtatgtg gttcaggtac
     6301 gctttgtatt gaagcagcct taataggcca taatatcgca ccaggattca atcgtgagtt
     6361 tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg gaaaatgttc gatcagcagc
     6421 cgaagaaaaa gcagattatg atattgaatt agatattact ggttcagata tcaatggacg
     6481 gatgattgaa attgccaaag ccaatgcaga agaaatcgga ttaggctctt cgattacctt
     6541 taagcaacaa gcagtcaaag acttcaaaac ggataaagaa tacggggtca tcgtggctaa
     6601 tccgccttac ggagaacgtc taggagaaga agaaactgtt cggaaattat ataaagaaat
     6661 gggcgatgta ttccgtccat taaaaacatg gagtaaatat atcttgacta gcgacttagc
     6721 gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt
     6781 gcgtacggat ctattccagt attggggaac tcgtccgcct agaaaaaaag agaaagacga
     6841 ataatcttac taattgattc attcaattgg atgtttggag gaatagggaa tgaaagaaaa
     6901 agagttttta caagaagtaa aagaaatcaa tcttctacaa caagctttag gaatcctcga
     6961 ttgggataca caaacaggta tgcctgaaaa agctagtgca taccgaagtg aagtagacag
     7021 ctatctttac agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg
     7081 ctatttttct gaacatccgg aagaattgtc ggaagttgga actgccgttt ttgccaaagt
     7141 aaaagaagag tatgatttgg aacatcgcgt accggaagat cttatgagaa aatatacagc
     7201 tgccgtttct tcagcacacg gacaatggca aaaagctaga gaaacacaac aattttctga
     7261 ttttcaacac gcattgaatc aaaatattga attaacaaaa cagttgatac cttactggag
     7321 aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa tacgaacccg ggatgactgt
     7381 cgaaatcctt gataaggtat ttgatcagct taaagaagga attctttcga tccgccgtac
     7441 gctccaagaa aaagggactg aacctgaaac agacttttta aatcggagaa tgacgaaaaa
     7501 acaacagaag agatttgtag tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg
     7561 tcgtttagat gacactgtcc acccatttat gactggaatt aatccgaatg atgcacgaat
     7621 cacgacacga tggaacgaaa cagatttcaa aatggctgtt tttggagtga tccacgaggc
     7681 tggtcatggt atgtatgaac aagacatcga tgataaatat gcgtatacac cggtttatga
     7741 aggtacatca atgggtatcc atgaatcaca atcgttgttt aacgagatca ttattggaag
     7801 tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag aaggaacatt
     7861 cgatgatatt ccttttgaca aattttatcg ttcattgaac catacaaaat caagtttggt
     7921 tcgtatcgag gcagacagtt tgacttaccc tctccatatc atcattcgtt atgaaatcga
     7981 aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa
     8041 atatgaagag tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt
     8101 tcattggtca ggtgcaagct ttggttattt tccctcttat gcattagggt acatgtatgc
     8161 ggcacaatta cttcattctt tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga
     8221 cgattattct ccaatcaaaa actggatgtc agagcatatc catcaatatg gtgcatctag
     8281 aaaaccaaat caattaatca tggatgcaac acatgaaccg cttaatccgc aatacctgat
     8341 cgactatatg aaagacctgt attttaatgt atatcaggta aaataaaata caatttacaa
     8401 agatagctga aattcatcta atctatagat gttttcagct atttacttaa gaaagaagtg
     8461 gaacgaatgg ataaattaca agtgatcgct gattatagta tggaccaatt agcgcatgat
     8521 caaacaggac atgggagcga tcataccaaa agagttgtca agttagctga aaaaattttg
     8581 gagacggaac cgcaggcaga ccgctttgtg acgcttgcag cagcttacct acatgatacg
     8641 attgatgata aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg tgtattcctt
     8701 cgctctttgc caatagtaga ggaacagatc tcgatgattt tttccattat cgaaaatatg
     8761 tccttttcta aaaatctctc tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa
     8821 gacgctgatc gtatcgaagc attaggagca atcggcattt tgcgtactgc ttatttcggc
     8881 ggtggacatg ggcacccgat ttttgatcct gaactgtatc cgcaaacatt caaggacaaa
     8941 gaaaattacc gaaaaggcac gacagtgatc aatcattttt atgaaaaact gttcttgctg
     9001 tctgataaaa tgaatactga ttacggatat gaagaagcca aacgaagaga aaccttcatg
     9061 cgtgaattct tagaagaatt ttttctggaa tggtctattg acgatgaaaa ttttactcca
     9121 gacaattgga tcaaaaataa caaaaaatga tggaggttgt gacagaagtg gtcagctcca
     9181 agatatttcg gatcattttc cgtaaaagtt acttctggaa cactgtttac caagaggtcg
     9241 tgagcctgcc gttcagctcc gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt
     9301 tgatgaatat tgtaacttgt gattcggaat tggcaggaga acaccgttta ttcggaagaa
     9361 taggactgtg acaagacttt tgtcacagtc cttttttcga attcaacaag taaaactttt
     9421 aagaaatatt cagagagtaa aattaaaaat tgctgataaa cgaagatgga aattttatgt
     9481 atttttattg gtctatgcaa taaataaatt aaatattcag aatacaattg attttaacga
     9541 aagggcttct atcaatggtt gtgttgtatt tatctgccca ggtagagtaa aatagatcat
     9601 tatctaaaag gtaattgttc atactatata agtcatttga aagtaaaaag atactgtaaa
     9661 taaaaaatgt ggttaaataa ttctcataat cagtgtaagc gttatcaaaa atatagcata
     9721 cttgctttgt agatgaatga cacgatatat gtttggtatg ttctgcaaga cagatgaata
     9781 tggaggtgga gacaatgtct tccaaagaac taacaatgta tttagagaaa gaattggatc
     9841 aactgaaaga aaaggggtta tacaatacga tcgacatact agaaagtgaa aatggtgctt
     9901 gtatcatcgt agacggaaaa aagatgatca atctagcatc caacaattat cttggatttg
     9961 ccaatcgcga agaattgaaa aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag
    10021 caggagcagt ccgaacgatc aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa
    10081 tcgcagaatt caaagggaca gaagcagcaa tcgcctttca atctggattc aactgcaata
    10141 tgggcgctat ctctgcagta atgacaaaag aagatgctat cttatcggat gaattaaacc
    10201 atgcatctat tattgacggt tgccgcttat ccggagcaaa aatcatccga attaaacatc
    10261 aagatatgaa agaccttgag aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa
    10321 aaatcatgta tatcacagat ggtgttttct ccatggatgg agacattgca cgattgccgg
    10381 aaattattcc gattgttgaa aaatatggac tgatcaccta cgtcgatgat gcgcacggct
    10441 caggcgtaac tggaaaaggt gcaggaaccg tcaagcattt tggattaagc gacaaaatcg
    10501 atatgcaaat gggcacgtta tcgaaagcta taggtgttgt tgggggttat gtagctggat
    10561 cgaaaacact aatcgactgg ttgaaagcta gaagtcggcc attcctattt tctacttcgc
    10621 taactccagg tgcagctgca gctgcattgg cttcgattac tttgatgcaa gaacatccgg
    10681 aacttgtcga aaaagtttgg gaaaatgcta attattttaa agaagaattg aaaaaagtag
    10741 gctataacat cggtatgtct gaaacaccta ttactccagt cattttggga gacgaaaaag
    10801 tgactcagac tttctctaag aagctgatcg aacatgggat ttatgctaaa ccaatcgttt
    10861 atccaactgt gcctcttggg acaggcagga tcagaaatat gccgactgcg gaacatacga
    10921 gggaaatgct tgatgaagca gtagcagttt atcaaaaaat aggaaaagaa atggaaatta
    10981 tttaactaaa tttagttttt tcgtctattt aaaattagag aacatgttca cgcatatatt
    11041 gttaatcatc tgaagatagt agtaaaaaag atcagggagg atcatcatga aaaaagtatt
    11101 ggtgacagga tgtttaggac aaattggttc agagttgatc acacggctac gtcaagaact
    11161 aggcagtgga aatgtcattg cgacagatat acgcatgcct gaagtcattg agaaaaacga
    11221 tatctttgag atcctagatg taatggatta cgaaaaaatg cgcagcatag cagaacaata
    11281 tcaagtggac acattgattc atttggctgc tcttttgtca gctgttgcag aagcaaaacc
    11341 acaattagcc tggaatttga acgtgactgg gctggtcaat gcattggaag tcgctagaga
    11401 attccagctg aaatttttta cgccaagttc aatcggcgcg tttggtccaa atactccaaa
    11461 agatcatacc ccacaagata ctcttcagcg tcctacaacc atgtatgggg taacgaaggt
    11521 agctggagaa ctactatgtg attattatta tgtgaaatat ggcgtagata ctcgtggtgt
    11581 ccgtttccct ggattgattt cttatgcaac cttgccagga ggcggtacga cagactacgc
    11641 agtcgatatt tattattcag caatcaaaga aggaaagtac actagtttca ttgatcaagg
    11701 tacatcaatg gatatgatgt atatgccaga tgcgatcgat gccattataa aactcatgaa
    11761 tgcagatcct gatcgattgg ttcatcgaaa tgccttcaat atatcggcga tgtcttttga
    11821 cccggaagag atcaaagcat ctattcaaaa aatcatacca gattttgaaa tggattatga
    11881 tgtggatcct gtacgtcagt caatagcaga atcttggcca aattcactag atgtttcttg
    11941 tgcgcaagag gaatggggct tttcgccgaa atacgactta gatgtgatga caaaggatat
    12001 gttggaaaaa ctgaaagaaa aattggtaga gacggtataa aacaagaacc acatccaaaa
    12061 agttttttat acttttggga ttggctatgg tatgaaaggg gacaagggat ggaagaatat
    12121 caagccacgc cagtaaaaga agtaaaaata gaaaatgaat taaaacgtac gatgggattt
    12181 tttactgctt tatcgactgt tatgggcaca gtgatcggtg caggggtatt ttttaaagcc
    12241 gcaagcgtag cagaagttac aggatccgcc agtttgcata tgttttcttg gtttctcggt
    12301 ggtatgattt ctgtctgtgc aggactaaca ggagctgaac tggcagctgc tattccagaa
    12361 acaggaggga tgattaaata tatcgaacgg atttacggaa atacagcagc ttttttattg
    12421 ggctgggcgc aagtcgtgat ttattttcca gcaaatgtag cagccttgtc gatcattttc
    12481 ggtacacagt tcgtcaatct atttggtctt tcccagtcaa tgattgttcc cgtagctgtt
    12541 acagctgctg tatcgatcct gttgatcaat tttttaggtt ctaaagctgg aggggctttt
    12601 cagtcgatta cgcttgtctg caagcttatt cctttgtttg tgatcgtcat tttcggtttg
    12661 ttccgacaag aaggagtaga cttccaactg tttccgatcc aagcaggaga aaatctttca
    12721 tttttctcag ccttgggagc gggcttgcta gcaacgatgt tcgcatacga cggttggata
    12781 cacgtaggaa atatttcggg agaattgaaa aagccggcga aagatttgcc aaaagcaatt
    12841 tctttaggaa tcatcggaat catgatcgtt tatttattag taaatgcggt gttcttaaga
    12901 accgcatcta tcgacggagt cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg
    12961 atttttggcg gcttcggtgg aagactggtc acagttggta tcttgatttc tgtttatgga
    13021 accatcaatg gctataccct tactggaatg cgtctccctt atgttatggc aaaagaaaac
    13081 aacctgcctt ttagcaagct ctttgctaaa cttcatgata aaactaaagt tcctgtagcc
    13141 gcaggtatct tagagctagt catcgctatt ggcatgatga tgatcggtgg ttttgataca
    13201 ctaacggata tgctgatctt tgtcatttgg attttttata cgatggtctt tgttggtgtc
    13261 attcttcttc gtaaaaaaga acctgatttg tttcgaccat acaaagttcc aatgtatccg
    13321 tttattccac tagtagcaat cattggaggg acttttatcg tatcaagcac gcttatcact
    13381 cagacctttt tagcttcgat ggggatagta ttgacacttg ctggtatccc gatttatctc
    13441 tatttaaaac gtagatagct aaaacattcg aattttatta gaactgcact tctctgccaa
    13501 acaaaatatt ttatttaaat gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt
    13561 ttgatcacgt aaatgtcgtt ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg
    13621 gctcaaccga tttctgtctt ttactttgat ttgcgtaact gaactattcg gcgtacgccg
    13681 cgataaatat ttaataaaac caagcaacca gaaatgaaaa tgggaagcca aatcagatag
    13741 acagggtagc caagcacttt agacaaagca tgtggagtca ttttatccat ttcaagaagt
    13801 gcttgctgcc aagtctgaag attgaagact agcatgaaac ccgaaataat caggagaata
    13861 aatcctaaat aatgaattgt agttcttttt cttacataag cgatcgtagc ataaaggaac
    13921 agtaaaaaaa taggtacgcc aaaaacaaag tacatattca gcatctcgac accccctttg
    13981 tgtttatctt actttacttt gaaagaaatg agaaataata gcctcgttac ttgcttgaaa
    14041 cagcaaagga ctgctaagat aagagagata caaagaatca aggaggagtc atatggcaag
    14101 ttatatcgaa ccgattcgtt ttgcgctgat tgtttttcca tttttagcct tagcgatctc
    14161 atctgttttt tttatttatg aatatcgaaa gtatggaaca tttctagtaa caagggcagt
    14221 tattttgtat tctttcgttt tttatttatt gtgtgcgtat tttttggtga tcttaccact
    14281 tccggcaaag gagacggttg cccaaatgac agggccaaag atggagctgc atttatttgc
    14341 ttcatggcaa catttcagag acaacacggt attggtcttg tctgaaccta gtacctacct
    14401 gccggcaatg aagcagagtg tatttttgga accagctttt aatgttttat tactcatacc
    14461 atttggcatt tatttaagat attatttccg gctttctttt atgaaaacga tgattgctag
    14521 tttctgcctt tctctatttt ttgaattgac gcagctaagt ggactttatt ttatctaccc
    14581 aagaccatac cgcttatttg atgtgaatga tttagtgaca aatacattgg gtggaatcat
    14641 tggttttgtc gttacgccgt tatttacatt tatgcttcct acaagaagac gaatggatga
    14701 actttcttat gaaaaaggaa agactgtctc attgactcga cgcttggtcg cttatttgat
    14761 cgactggaca tttttatctc ttattaattt ggtagcggcg attttcttta aaattgcgtt
    14821 cgattcaggg gcagttactt ctcatatctg gtggctttta ttagaagtag gggtatactt
    14881 tatgattgta ccgtttataa ccaatggatg gacacttggc aaaaaaattg tgcgtatccg
    14941 tttggtggaa gaagaccgcg aacgaatttc tttaaaagca ttgatgatcc gctacgggta
    15001 tctgtacttt cttttttatg gtttaagctg gttggggcag ttgcttcagc aaatcatgaa
    15061 ttcgacaacg aatacggtgt tgcagatgag tgcagtattc atttctctcg gtgtcttgtt
    15121 actacaaggt atttttattt taaatatctt gctatcattt ttgagaaaaa aacggattct
    15181 gttttatgaa aaagctagtc acacatatac tgttagcacg attggccaaa caaaggatga
    15241 tgattctcga aattagctga gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga
    15301 taatttaaag agaagaggtg cacgaatgaa taattttgat atattccgct tcaaaccgtt
    15361 tatcaatgaa gcgttaaccg caaagggctt caaacagccc acagaggtcc aggaacgttt
    15421 gattccttta attgcaaaag gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa
    15481 aacacatacg tttttgttgc ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca
    15541 agttgttatt acagctccaa gtagggaact agctgcacag atctatgaag cagctacaca
    15601 aatcgctaaa ttttctacac cggagatccg tgtctcgaat tttgtcggcg gtacagacaa
    15661 acagcgtcaa ttaaatcgtc tgcatcacca acagccacag gttgtagtag gcacgcctgg
    15721 tagaatatta gacatgatga atgaacaagc gctgaagatt cacaccgcat ttgcatttgt
    15781 tgtcgatgaa gcggatatga cattggatat gggtttttta aatgaagtag accaaattgc
    15841 aagtcgatta ccagaaaagc tgcagttttt agttttttct gccactattc cagaaaaact
    15901 tcgtccattc ttacgaaaat acatggaaaa tccaatcatt gaagaaatca aaccaaaagc
    15961 agttatttct gaagacattg aaaattggct gatttcaaca aaaggtaaag atgagaatca
    16021 aatgatttat caactgctga caattggtca tccttattta gcaatcgtat ttgccaatac
    16081 aaaacagcat gtggatgaaa ttgccgatta cttgaaaaac caaggcttga aagtagcaaa
    16141 gattcatgga gacatcaccc caagagagag aaaacgagtg atgcgacaag tacagaatct
    16201 ggagtatcaa tatgttgtag cgactgattt agctgctaga gggatcgata tcgaaggtgt
    16261 ctctcatgtt attaatgcag aaattccaga agatctagac ttctttatcc atcgtgtcgg
    16321 acgtactgga cgtaatggct taaaaggaac ggccattacg ttatattcgc caaatgatga
    16381 gcaagccatt gatgcgatag aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa
    16441 cggggaaatc gtcaaaacat acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga
    16501 tgtattagat cccacgctga ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg
    16561 ttataagaaa aaaatcgatt gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga
    16621 acgcagacag caaagccgta ctgctcgtaa aaataagaaa aattcacata aataaaaaac
    16681 aagaggttgt gacaaaaggc gtgtcacaat ctcttgtttt taagctagac tttttttcat
    16741 tccgatacaa agtacttcat cttcaataaa aggttctgtg caagtatgat acccttgttt
    16801 ttcataaaaa tctacagccg tcatttcagc tactacataa ctagtctgac aaccttctga
    16861 aaccgcccgt ttttcagcag cttcaagcaa ctgaccgcct aacccttttc ctcggtattc
    16921 ttttgctacg caaaatcgtt ccggcttgaa gcaagtaggt gttttctttt cataacgcaa
    16981 agtagctact ggtctttttt gatcaagaaa tagataatag gtttgttgcg ggatatcttt
    17041 tccgtcgaat tctagttctg caggaatccc ttgttctaat acaaaaactt gatatctcaa
    17101 aaagcagcta gctgcgcgta tccattcatc cgctccaact atttttttca tcatattcct
    17161 ccttgtcatg aatagcagag atgtattgat tcctttattc ttatcgtacc aaaaatcgtg
    17221 gagtatgacc aaaaggaaat gaaaataaaa attgagatat tgacagtgat caatagaatg
    17281 cgtatactaa ataagtacga aaaggatatg atttgttttt taagactcac agaaaattct
    17341 ttcttggctg aaaaaagaat agtcactaat caaattgctc cctttttcta ttttggtaga
    17401 aataccgacg cagctcagcg ttatcgagtt taagaggtaa tgattacaca tcattgcaat
    17461 caaggtggta ccgcgagttt tcgtccttgg ctgtgatggt gtttttttgt atttaattta
    17521 tagaaagcag gtaatgacat gaaacaatta agtagtgcac aagttcgaca aatgtttctt
    17581 gatttcttcc aatctaaaga acatacgatt gaacctagtg cgtctttgat ccccgtgaac
    17641 gatccaacct tattgtggat caactctggt gttgcaacat tgaaaaaata ttttgatggt
    17701 tcagttgtgc cagaaaatcc aagaatcaca aatgcccaaa aatctattcg aacaaatgat
    17761 atcgaaaatg tcggtaaaac agcacggcac catacgatgt ttgagatgct gggtaatttt
    17821 tcgattggtg actatttcaa aaatgaagcc atccactggg catgggaatt tttgactgat
    17881 ccaaaatgga tggcattcga tccagaaaaa ctttatgtaa cagtttatcc aaaagataca
    17941 gaagcaaaac gcatctggca tgaagaagtt ggcctgccac tagatcacat tattgatgta
    18001 gaagacaact tctgggatat cggtgcaggc ccatgtggtc cggatacgga aattttctat
    18061 gaccgtggag aagcttataa cgatttagca gaagacgatc ctgaaaacta tccaggagga
    18121 gaaaatgaac gttatttaga aatctggaac ttagtatttt cagaattcaa ccataagcct
    18181 gataacacat ttgaaccact tcctcataaa aacattgata cggggatggg attggaacgc
    18241 atggtctcaa tcgttcaaga tgcgtcaacg aactttgaga cagacttgtt tatgcctatt
    18301 atccacgccg tagaaaagct aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat
    18361 atttcttaca aagtaatcgc agatcatatt cgcgcccttt catttgctat tggcgacggt
    18421 gcattgccat cgaatgaagg gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc
    18481 atgcatggga aaaaattagg tattgaacaa gcattcttat atcgtttagt ccctgttgtt
    18541 ggggaaatca tggtaagtta ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa
    18601 gttatccgta cagaagaaga acgtttcaat gaaacaatca atgaaggttt ggaaatctta
    18661 aatcaagtga tccaagaagt aaaaaacgaa aacgggacga ctctagcagg aaaagacatc
    18721 ttcaaactat acgacacata tggcttcccg gtagaattaa cggaagaggt ggcagaagat
    18781 gaaggactga aagttgatca tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt
    18841 gctcgctctg cccgcagtac tgaaaaatca atgggggtac aatctgcttt attgacagat
    18901 atcaaagtag aaagtaaatt cattggatac acagaacaag aatcagatag tcaattattg
    18961 gtcatcgtta aaggtgagtc tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc
    19021 tttgatcaaa cacctttcta tgcagaaatg ggtgggcaag tagcggatca aggatggatc
    19081 atgaatgaag taggcgaaat cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt
    19141 caatttatgc acaccgtaga agtaaaagca ccaatgagtg aaggacaaac ctatcatcta
    19201 ttgatcgatc aatcgatgcg caatcggatc atcaaaaacc atacagcaac ccatctactg
    19261 cacaaggcat tgaaagaagt acttggcgag catgcaaatc aagctggttc tttagtcgct
    19321 cctggacatt tacgattcga ctttacccac tttggtcaaa tcacttcaga tgaattagct
    19381 gaaatggaaa gaatcgtcaa cgaaaaaatc tgggaagcta tcccagttga aacaattgaa
    19441 acagatatcg atacagccaa aaacatgggt gcaatggctt tgtttggtga aaaatacgga
    19501 catgacgttc gagtagtaaa tattggcggc tggtcaatcg aactgtgcgg aggcacacat
    19561 gtagcaaata cagaaaacat cggaatcttc aaaatcgttt ctgaatcagg aattggtgca
    19621 ggcgtgcgac gaatcgaggc tgtaacaagt aaagaagcgt acgaactact tgaagaagaa
    19681 gaaaaacaac taaaacaagt agcaacaatt gttaaatcac ctcaattgaa agaagttgtt
    19741 tcaaaagcgg aacaattgca acagcaagta agagagctac aaaaggaaaa tgaacaatta
    19801 gctagcaagt tagcgaatca acaagctggt gatgtattca aagatccaga aacgatcaat
    19861 ggaaaaacaa tcattgcagc acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta
    19921 gctgaccaat ggaaacaaaa aaatatttca gatattcttg ttttagcaaa cgaacaagaa
    19981 ggaaaagtca gtctgctagc ggcaatgacg aaagaatcaa atgaagctgg actaaaagct
    20041 ggcgacttga ttaaagcaat cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac
    20101 atggcacaag ctggcgggaa aaatcctgct ggtattccag atgcacttaa agctgcgaaa
    20161 gaatgggttg cgcaacaata gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt
    20221 gaacgaaaca tgattatggg ggcgaagaca aacaagtctt gccctcatac ttttttcaat
    20281 tggatggcgc atctaatcaa atggtcggat tcggatggtc tttagttgac gaaagtgaca
    20341 gaaattttta cgattagtat aaataaaaag gggtgaaaaa atgaatagta gaaataagac
    20401 ctttcgacta gtcttacgtg cgatcttgtt agcaatcatc atcgtacaag cgatggttcc
    20461 ttggctgggg tttattccat taggattcat tagtttaaca attatccata tcacagtaat
    20521 tattgctgca gtagtattag gcccaaaaga tggcatggtg attggcttgt tttggggaat
    20581 cgctacaatt gttcgtgcct atgccatgcc aacaacgcca tttgatacat tagtgttcac
    20641 gaatccgatt atctcggtag ttcccagagt acttgttggc ttagtagcag gcttggtttt
    20701 ccactggatt tatcaaaaaa ataaatcaat aacgatcagc tcagcctttg caggggtact
    20761 gggttcattg gtcaatacag tcttagtgtt aggattcatg ggattatttt atacaggtgc
    20821 aacggcagat gcctatggtg tagactctgc tcttttattt aaaaccttag caggggttgc
    20881 agcaatcaat gggataccag aagcaatagg tgcgggaatc ataacaccat tactagcaaa
    20941 agcgttattt gcagcaacac cgttgaaacc agaataatga ccatctttta atagtaatag
    21001 gacgcacgat agatagaaaa aggaagagag gagaggaaaa caatcgtcct ctccatcttt
    21061 ttacctgagc aacaagcaaa ctagaagggg taaaaaactt ggctaagaaa caagaagaaa
    21121 aaaagaaaca aataccgcaa cagcggacaa aaaaaggagt actagcgggt atcttaggat
    21181 tggttacgaa tctgatcttg tttgtctcta agctaatgat cgggcttttt tcaggaagtg
    21241 tctccattat ggctgatgca gtgaacagtc tatcagatac tgcttcatcc atattgactt
    21301 tgatcggatt taaaattgct gcaaagccag cagatcagga acatccttat ggacacgaac
    21361 gcttcgaata tatcagtggg ttatttgttt cgatcatcat tacttatgta ggatttcaat
    21421 ttttagagtc ttctgctgaa aaaatcatcc atccagaaaa tgtctcttta acacccatcg
    21481 tcttttttgt cctaatcatt tccatctttt tgaaatttgc acaaggcaaa atgtatcagg
    21541 ttattgcgca atccattgat tctgaaacat tgagagctac agcaacagat agttataatg
    21601 atgtctttac tacactggct gtattgcttt cagcagcaat cgaatgggta actggatggc
    21661 gtattgatgg atatataggt tttgctttag cattatatat cctttatagt ggattgacga
    21721 tgcttcgaaa ttttgtatac gaactattag gaagtcgccc aacgccagca gagattgaag
    21781 aaatggaaga gcatttaaat gcttgcgatg atatacttgg ctatcatgat ttattggtgc
    21841 ataactatgg acccaataaa cgctttgcat cagtccacat cgaagtagat gaatcaatga
    21901 atttgaatgt ggctcataag attattgatc tgatagaaaa agactttaaa aagtctttgg
    21961 atgtggaact tgtctgtcac ttggatccaa tgcctgttga taacgaaaaa tactataata
    22021 tcttgcggca gttaaagcaa attcttcttg aaacaggtac agggcttaat attcatgatt
    22081 ttagagtaat tcatcaagga aaaacgctgc aatttgatgt agtggtccct gagaaattcc
    22141 agtttgttga tacagaacta gaaagaatct tacgtagtca aatagaagaa aaaatcggag
    22201 attaccaatt ggaaattacg tttgatcaca attatctttt atattaaatg agaaactata
    22261 aaaagactcc ttaaagtttt taatttaaag agtctttttt tcttttaatt gtttatggag
    22321 agcctgacgt gcaagattat ctgctccttt atttttgttt tcgggcaacc attgcaggat
    22381 caataaagaa aattcggata gaagttcttg gattctttga aggtagggaa ggaagagctc
    22441 gttcttcgtt gcgtttttgt cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa
    22501 aatcgtttca ttctgtaagt cgtttttctt tagaaattct aaagttttta aaaagacttc
    22561 gaactctgct tgatggtttg tccctataga aagcgggata gatagctgtt cttgtttacc
    22621 ttgatagaca atcaagattc cacctccact aggaccaggg ttgccttttg tagaggcatc
    22681 aatgtatact tttaacataa aaagagattc tcctttttgt tcaaactaat gttatggtaa
    22741 gattatatca ttactgaatc ggtgagaggt tagagaatga aaaaaaatat tcgatggcag
    22801 cctgaactgg cacatacgat catctattgg tcattgacct tgatcattct gttttttagt
    22861 ctgattcttt ctttagaaaa cacaggtcct tattggaaaa gcaattttgt tttaggtatt
    22921 tttcttgttt ttctatttct aggtttgcgg cgttctttta ttttaaaatc aaactctgtg
    22981 attatacgtt atgctgcttt ttggaaagac cgagaaatat ctgtcggaca gattcgatct
    23041 ttgacgccaa ctaatactgg ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt
    23101 ttcatgatgc gaaaaaaaga tcaagtagtc ctttttgatc attttgaaaa aaacggaccc
    23161 aaatcagaat caattcctta tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca
    23221 gaacctaaaa aaacgaattt ttacaggaaa agaaaagagt gagacgtacg gttcatcccg
    23281 tttagcgtct cactctattt gtttatctat tcatgcatac tagtatcggt agtctgaacg
    23341 ttgcctttct tgacatgggc agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg
    23401 accacttgat tttcttccaa agttttgaat ccctcttctt caatcgcagt aaaatgtaca
    23461 aaaatttctt ctgtgtcatc ataactgata aagccgtatc cttttttgtt gtcaaaccat
    23521 tttacaattc ctgtggtcaa agcactcacg acctttctaa agtctgatca tagccttcat
    23581 tataaaga
//
LOCUS       DACCWK010000035        22686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCWK010000035 DACCWK010000000
VERSION     DACCWK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.035"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            62..208
                     /locus_tag="IVW65_001321"
     CDS             62..208
                     /locus_tag="IVW65_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP7900207.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            284..1531
                     /locus_tag="IVW65_001322"
     CDS             284..1531
                     /locus_tag="IVW65_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7900208.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            1546..2298
                     /locus_tag="IVW65_001323"
     CDS             1546..2298
                     /locus_tag="IVW65_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900209.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(3001..3216)
                     /locus_tag="IVW65_001324"
     CDS             complement(3001..3216)
                     /locus_tag="IVW65_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7900210.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3235..3471)
                     /locus_tag="IVW65_001325"
     CDS             complement(3235..3471)
                     /locus_tag="IVW65_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7900211.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3729..5882
                     /locus_tag="IVW65_001326"
     CDS             3729..5882
                     /locus_tag="IVW65_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP7900212.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5851..7266
                     /locus_tag="IVW65_001327"
     CDS             5851..7266
                     /locus_tag="IVW65_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP7900213.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            7299..8333
                     /gene="sppA"
                     /locus_tag="IVW65_001328"
     CDS             7299..8333
                     /gene="sppA"
                     /locus_tag="IVW65_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7900214.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            8335..8856
                     /locus_tag="IVW65_001329"
     CDS             8335..8856
                     /locus_tag="IVW65_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7900215.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8853..9239
                     /locus_tag="IVW65_001330"
     CDS             8853..9239
                     /locus_tag="IVW65_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7900216.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9735..9807)
                     /locus_tag="IVW65_001331"
     tRNA            complement(9735..9807)
                     /locus_tag="IVW65_001331"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9772..9774),aa:Lys,seq:ctt)
     gene            10188..10757
                     /locus_tag="IVW65_001332"
     CDS             10188..10757
                     /locus_tag="IVW65_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7900217.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10744..11604
                     /locus_tag="IVW65_001333"
     CDS             10744..11604
                     /locus_tag="IVW65_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7900218.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11794..12498
                     /locus_tag="IVW65_001334"
     CDS             11794..12498
                     /locus_tag="IVW65_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900219.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12503..14338
                     /gene="walK"
                     /locus_tag="IVW65_001335"
     CDS             12503..14338
                     /gene="walK"
                     /locus_tag="IVW65_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7900220.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            14335..15651
                     /locus_tag="IVW65_001336"
     CDS             14335..15651
                     /locus_tag="IVW65_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900221.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15652..16521
                     /locus_tag="IVW65_001337"
     CDS             15652..16521
                     /locus_tag="IVW65_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900222.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16576..17385
                     /locus_tag="IVW65_001338"
     CDS             16576..17385
                     /locus_tag="IVW65_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7900223.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17488..18117)
                     /locus_tag="IVW65_001339"
     CDS             complement(17488..18117)
                     /locus_tag="IVW65_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP7900224.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            18287..19579
                     /locus_tag="IVW65_001340"
     CDS             18287..19579
                     /locus_tag="IVW65_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7900225.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19655..20317)
                     /locus_tag="IVW65_001341"
     CDS             complement(19655..20317)
                     /locus_tag="IVW65_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7900226.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20542..20826
                     /locus_tag="IVW65_001342"
     CDS             20542..20826
                     /locus_tag="IVW65_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7900227.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            20877..22502
                     /gene="groL"
                     /locus_tag="IVW65_001343"
     CDS             20877..22502
                     /gene="groL"
                     /locus_tag="IVW65_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7900228.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7773 a         3601 c         4516 g         6796 t
ORIGIN      
        1 gaaaaatagg tatattgaat cagtgacata aaagtattta tagtttgtag ggggaatcgt
       61 aatggaagaa aaaaatagac taaatgctaa acaatgttca gatcaagagc ttaaaaaaat
      121 taaaggaggt gctggaacaa aacctcaagg aaaaccggca tcaaatttag tggaatgtgt
      181 tttttcatta tttaaaaagt gtaactaaaa ttttttttat gatatcctaa aatcaggagg
      241 ggatctcttg attactatcg atacaaaagc ttatctatta agaatgttcg tgggaaactt
      301 tttctttttt ctcttatttt atatgactaa tactgatctt aacaaaagaa aatatctttt
      361 tgtatgttta ttgataccac tcactttttt tgttgaatta tttactgatg tttccgcagc
      421 tatacctata atcggaagtt attttatatt aaaggaaaag aagaaaacta atatagtgct
      481 tttaaataaa ttactattat gtatgcttat tgatgatttc agatctatta ttacttccat
      541 ggttatgacg tatatgtttt catttgaagg gataaaaggg tatggttatg tagtattcca
      601 gataatgctt gattctttgt ttttgctgat gtttatttgg ttatatagaa aattagctat
      661 aaataggatt gttgaacagt ccagttctaa acttactgct atattcatga tttatttact
      721 tacaattggt ttatttgttt cttatgttgc tcatcaatat caagtatttg atcattttgt
      781 gtttggtgtc ttaatatttt taatcattca aatgtttgtt gttctatttt tatttattag
      841 aataactatt aggcaacgag aacaatatga acagcaattg aaaagaaaag aattggttta
      901 tctaaaaaaa tatactgatt cactggaaaa agatcaagaa aaatttgcca gatttagaca
      961 tgactataaa aatttattgc tgagtttaaa agaaatcgct aatcaaaacc atgattcgat
     1021 attggaaaaa gaaataaaag aattagagat ctattctaat tcttatctaa ccaataagtt
     1081 tgaatatcgt tatttaaaaa atataaaaaa tgaatatttg aaaagtttgt tgatagcgaa
     1141 gctataccaa gcaaataaca ataagatttg ttgtaaattc gaatgtccaa gtactattat
     1201 tgaagttcct gttccaatat ttgactgtat tagggtgctt ggaattattc tcgataatgc
     1261 aattgaagca tctatagaga gtcaagatag agaactgtct ctagctattt accgagatga
     1321 taaacagata gaaatttcga tatcgaatag ttgtagagag atactactct ctatagatac
     1381 tctaatgaaa aaaggaataa caacaaaaaa gggacatcaa gggttaggtt tatctagtat
     1441 agaggaaatc aataaaaaga atcccaatat gtttgtgaac tatcaaaacg atccgtatca
     1501 atttactact caagttattt tgttgttttg agggaaggag gatttatgag ttatccaata
     1561 atattatgtg aagatcaaat tatacaatta aatcagttag aacgaattat tgataatttc
     1621 attctttttc acgataaagt atttaagata gtattgaaaa cacaaagtcc gttagaagtt
     1681 aaaaaatacc tcaaacaatt ccgaccaaag caaggaatat atttcttaga tattgattta
     1741 aatcatgaag ttaacggtat agaattagca gaagtaatca gaaaatatga tgttcaagca
     1801 aaaatcattt ttacaactac tcatgatgag atgttacccg taacaataaa aagaagagtt
     1861 gaaacgttag gatttgtaac aaaagatcaa acactagatg agtatcgaaa cgagattgtt
     1921 gagttattgt tattagcgca agaaaggata gatgcaacaa aacaggttac gaatcaagca
     1981 tttgtttttt ccataggttc tcaaacattt acttttgata taaatgacat ttattttgta
     2041 gaagcttcaa aattaccaca tagattatca ttgtgtacta aagatgggca gtacgaattt
     2101 tatggaagaa ttagtgagct tgaaaagaaa tatcctatgt taacaagaat tagtcgagcg
     2161 tgtttagtaa atatctttaa cgttaaagaa atagatttta aaaaacgttc actatatttt
     2221 gattcagagt tggccagaaa ctttacttta gggaaagcac aaaaaattaa ggagaagttg
     2281 aaagaaagca ctgtttagtg gttttttctt tagggagatt gcatttttct aataaaatag
     2341 attccttcac tagtgcaaat ttttctgaat cctattaaag ttgtaccaat ttttatagct
     2401 gttattagtt acgtgcataa cagcaatatt agttactgga ggaatatttc tatggtctgg
     2461 aaccaaaaaa gggccaaatg aatagttctt ttcttgtttt tattaagtac cataaagggg
     2521 aattaaaatt aaatgtatgg tggaagatgc gaaagatttg gcaaattcta ctaattttta
     2581 gaaagtgttt ttcatttttt agttaaaaaa tttgtcctgt caagtgcaat cctatgattg
     2641 tacttgatag gatttgagat aattactttc ctgtttttat aaatttaact tgcaggaaca
     2701 ggctttaatg cacatccgac aagtacatta ccaatacctc ctgtaacggc cgaaataggt
     2761 cctcctgcag caccaactac agcgctgcaa gtccttttgt aaaaatatct ctaccattac
     2821 atttccaaag aatttttatc tgactttaat aaaagataaa aatttaatat aattataaaa
     2881 actagcacaa gaagctttag taaaaagttc tgtccattcc aaacttttga aaagataaag
     2941 aacataccta tgttcgtaat cgacacaaac caatcttgag gtttgatatt ttttaattgt
     3001 ttacgcaccg gtccacatgc tgaatccagc acctaataaa gctccaccta caccacaagc
     3061 agctattgca taaggtcctc cggctgaaca aagttttact cctgctgttg ccccagaaaa
     3121 agtagtgcct aaaactccca cggcactccc accttgaata tttttcaact cttctgaaga
     3181 taatataaat gttgtttttg tgtttttaat atgcatacta cttattctcc ttttttattt
     3241 ttttcgagca tcccagccag ctgcagcacc tccagcgaca gctccatata accctccaac
     3301 aacaccgcaa gctggtccgc aaattgctgt tcctaaacct gcaccaccta ctgcccctgt
     3361 ggtaagtcca cctacaactt gccccaattt tccccaagca ttattgcctc caccaacagt
     3421 ttgtgcattt ccacaatact tagttccttt acattttgca tgtttaaaca cgcttcttta
     3481 aataatattt tttacggtgc tactgtaaca tacgtatttg attaacagta aaaatcttcc
     3541 tgaaacttat caagtttatt cttgaatagt aaaatttcca aaataaattt gattcaggaa
     3601 tgaaaaagtt agtgttcaag aactgttttc aaagtttttg tggaattatg agacaatatt
     3661 ttcactgtat taaagctata ttgagaattt ttctataaaa attttttaga aggagttgtg
     3721 ttagataagt gataaaatca tttccttttg tgcagcaaca agatgaaaaa gattgtggtg
     3781 ttgcttgcct ttcgatgatt ttaaaatatt ttaaaactga tatcccgatt cataaattac
     3841 gggacctatc tgggactgat ttagagggta cttcagcatt tggattgaaa aagacatttg
     3901 aaaaattgtc ttttgattgt atggctattc aagcagatag tgagatctgg aaagaaagag
     3961 atcttatctt accattaatt gcacatatat tggttgataa cagctatatg cactacgttg
     4021 taatttataa aacgaaggga aacgtactat ggattgcaga tcccgctaat ggaaaaaata
     4081 gaaaaacgat tgaagcgttt caaaaagaat ggacaggtat tcttttatta cctatgccta
     4141 gagaaactta tattcctatt aaagaaaaag tagctggatt aagttctttt ttgcctataa
     4201 tttggaaaca aaaaggatta gtattccaca tcgttttagc atcgattttc attacgtttt
     4261 ttggcatagg aagctcatat tattttcaag gtattcttga ttttttcatc cctaatcaag
     4321 cacgttcaac tttaaatata atttccattg gcttgatcgc tgtctattta tttcgggtat
     4381 tgtttgaata tagtcgtagt tatcttctta ttattttagg acagcgaatg agtatggcaa
     4441 ttatgcttca gtatttcaaa catgtgttaa ctttaccaat gaattttttt gctacgagaa
     4501 aatcagggga aattatttct cgttttcttg atgccagtaa gattattgat gctttagcaa
     4561 gtgctacgtt atcaatattt ttagatattg gtatggtgtt gcttgttggg gttacgttag
     4621 ctattcagaa tagtacgttg tttttaatca ctctatgctc tttgcctttt tatgtagttg
     4681 caatattcgc tttcgtgaaa agctatgaaa aatcgaacag ggaagaaatg gcagccggtg
     4741 cagctgtaaa ttctagtatc atagaaagtc ttaaaggaat agaaacaatt aaagcatata
     4801 gcggtgaaga gaaagtatat aaccgtatag accatgaatt tgttaaatta atgaaaaaat
     4861 catttcgtac ggttacgctt gataatattc aacaaggaat taaacatttg attcaattaa
     4921 ttagcagtgc attaatttta tggctaggtt cttattttgt tatggataat agaattagtc
     4981 taggtcaact tatcacatac aatgctttac tagtattttt gacagatcct ttacaaaaca
     5041 ttattaattt acaagtgaaa atgcaaacgg ctcaagttgc aaataatcgt ttaaatgaaa
     5101 ttttcgcgat tgattcagaa aaatcgaacc agtcttttga atatatgctt tctgaacaat
     5161 tatttcaaca agatatcact gtaaaaaacg tttcattctc gtatagcatg aaggccccaa
     5221 cgttgaacga aatttcatgt acaattcctt cgcacagtaa aacggctata gttggagtta
     5281 gtggttctgg aaaatcaact cttgcaaagt tatttgttcg tttttatgaa gcatcagaag
     5341 gatcgatcca ttatggaaac attaatcact tagacattcc ttttcagata ttacgcgatc
     5401 gagtgacata tttgcctcaa gagtcatttt tctttagtgg aacaatcatt gagaatctta
     5461 tattcggttt agatagccca ccaagtttcg aacaaataat agaagtttgt gagatagtcc
     5521 aactaaatga atttattaat caacagccat tacgttacga gacattgttg gaagaaggtg
     5581 gaaataatct ttcaggaggg caacgacaac gattagcctt agcacgggct ttattaaaaa
     5641 atccggacat attaatttta gatgaagcaa ctagtggatt agataccttg ttagaacatg
     5701 caattatgaa ttatctcctc aatttaaaag aaaaaacgat tatttttatt gcgcatcatt
     5761 taccaatagc aaaagcatgt gatcaaattt tagttctcca tgaagggcat ttggtagaaa
     5821 aaggaacgca cgatgaactt cgttttgccg atggtatcta tcaaaaatta tgggaaatct
     5881 aggagaggga aacaatttat gcagaataat aattggttcg aacattctag tatctatact
     5941 aaacaacatc atagcttcta ccgttgggtg ttataccctg taattctttt tttatgtatt
     6001 attggattgt ttttaacctt tgctaaaaaa gaggttgtta ttcggacaac ggctcaattg
     6061 aaccccgaaa aaatagagaa gttacaagtg ccattagaag ctaaaataat ggaaaacttc
     6121 ctaaaagaaa atcaatttgt tcataaggac gatgtgttgg ttagattgga ttgttcttta
     6181 atagagaatg aaaaggctca gatagaacaa gaaaaccaac gtattactca acaaataaag
     6241 atggctcagc tatttattga aagtataagt aaaggaaaaa atttgttttc aacggatgac
     6301 agttttggct acagtaatca attaaagagc atgttgtcag aaaaagaatc actccgctac
     6361 gctttgaagc aaagtgaatt aaatgatcaa aagcaattag aagtatacga aaagacaaaa
     6421 agacaactag aaaaacaaat tgagagttca gatagtaaat tacaagaatg gcaacaagta
     6481 caggtagctt ggagtaataa tcaatcatta aaagattttt caaaagaaat gatggcaaac
     6541 tatgagaatt ggcaagaaca actaaataat gtttctgatg atcaaaaaaa tcaagtgaaa
     6601 ctgacaattt cagcaagcat aaatgaacaa attgagcaac taaaaaaaga agtagaacag
     6661 tatcagtcag aaaaagctaa attagttaaa ccaactactt ctgagaatga cagaattagt
     6721 caaacggaaa aaggaaagca agagctagaa caagcaataa caactgtaaa acaaacagtt
     6781 acagagctaa atgaaaaaca agagaagaac cagtcagtta tcaaaagttt ggacgatcaa
     6841 ctttcaaaag gaatactaaa agctcctgta tctggaacgg ttcatttaaa tgaagaaaca
     6901 aagggacaag cagaactagc caaaggaaca gtgcttgcag aaatttatcc gatccacgaa
     6961 aaatcaaaaa tgaaatttac agcactttta cctgcgaatg aaagtaccta tattaaagaa
     7021 gggatgaagg ttcattttaa attggatcaa aaaggtgggg ctccgattac gatcgacgga
     7081 actttagatg aaatttcaga aaattctact gccactgaga aaggtgtttt ttatgcagtg
     7141 aaaggaacgt taaaaacaac tcaatcgttt ccgtttcgtt atggattaac tggagagttg
     7201 tctttaattg taggagaaaa aacatattgg caacaaattg tcgatgtgtt tctaaataaa
     7261 aaataattat ttttactaag aatataggag gagtttatat gaataaaaga agatggattg
     7321 ctgtgggagt agcgtgcgga ttattgttac tatccattat cgtcgcgcta attccaggaa
     7381 aagataaaga ggaagcatca aatacaaccc taacaggaat aaataagatt ttttacggat
     7441 ctaacgaaat cacagaagaa acgttagaag aaggcgcatc aaataaaaag attgtcaaac
     7501 tatccgtcaa tggagttatt gcagatacag gtgaaagcaa tctttttagt agggaacaat
     7561 ataaccatca gaatttttta acacagctaa agaaaattca agaagacaaa gcggttaagg
     7621 gagtgttatt agaagtaaat tcacctgggg gaggaatata tgaaagtgct gagattgcta
     7681 aagaaatggc aaaaattaaa aagctagata ttcctatcta tactgcattt aaaaatacag
     7741 cggcaagtgg agggtactac atttctgctg gttcagataa aatatttgct acggaagaaa
     7801 caacaactgg atcaattggt gtcattattt ctggtttaaa ttattctggc ttattagaaa
     7861 aacttggcgt aactgatgcg acgtataaaa gtggtgcatt aaaagatatg atgtcacctc
     7921 aacacaaacc ttcagaggaa gagaataaag ttatacagga atttgtgatg agtgcttatg
     7981 atcgttttgt caatgttgta gcaaaaggaa gaaatatgga tactaatgca gtgaaagaac
     8041 ttgcggatgg aagaatttat gatggtaacc aagcagtaga aaatgggttg gtggatcaaa
     8101 ttggttactc tgaagatgca ttagattccc taaaaaaaga gaagaaatta actgatgcga
     8161 cggttataga atataaaaat gacacaactg gctttgcttc ttcttggtta ggtaataaga
     8221 ttgctgaatg gcaagggcta aaggcaacta catcagatcg tgtaatttca gtttttgaaa
     8281 aacttggatt ttctgatact ccaaaaccaa tgtattacta tggagggcta taaaatgaat
     8341 aagaagagtg gcattgaaac tttgcctaat tatgtatttg cgggattttg gttacgattt
     8401 tttgcttttc ttattgattt aatttgtatt agcatcatca cccatgctac tataggtctc
     8461 ttttacaaaa tcatgggact ggaatctgac aaatccatta ttacttttta tggtatagcg
     8521 tcattagcta tttatttaac ctattttgta cttttgacta agctgaattc aggacaaaca
     8581 attgggaaaa tgatttttgg gataagagta gtgagctttg aggaaaaaga gctaagttgg
     8641 caaactatct ttattcgtga gtgtatatgt aggtttatat taaaaaattt tgtatttcaa
     8701 attgggtatt tggtggctgc tttcacgccc aagaaacaac atattgggga ttatttttct
     8761 aacacgtcag tggttgttat tcgttcatta gaagctatta actatggtca aaaaatttct
     8821 aattctaaaa cacttaatct ggagagaatt aaatgaataa gagaaaaaca gagtttattt
     8881 tgattttaat tagtggaata acagggattt tatgtagtct tttagcattg atgggtggta
     8941 tgtttctatc ttctattgat tcaactactt taaatactac agaactaacc agtaaggata
     9001 tagatatgtt agtcaatcta agcgggaatg ttgttttatt tggaggaatt accctagttt
     9061 tttcgataat gattttcata ataggttttt tagtaagaaa aaatcaaaaa acagtggtct
     9121 atggtattag tttactcatt ttatctatta ttccattttt tcttttaaca ttattgtggg
     9181 taattcctgg agttttaggt attattgcgg gattgatgct tctaataaga aaatcttaat
     9241 taggcaatgt gtagacaacg tgaaacgatt aatcaattaa aagctaaata cgttttgaga
     9301 aatgataact tgtagtcgat ggaagacatg tagataaaaa aactacgtgt cttttattta
     9361 ttctaatttt ttcgaaaaaa agacactctc tgattgttgt attaatggaa aaggggaaag
     9421 cagcaagtaa atttagtttt tctattaaat attcttcact ttttaacctt ttatagtact
     9481 tagatttaat cctatatgtt tagagagttc attggcagca tgattttgca aagcttctcg
     9541 aattattgtt atattagaaa ctttactgtt cataattatg cttctttttt aactcaaagt
     9601 ccaaagggct acagtggact ttgagttaaa aataaaaagc cctagttatt ttgaaaaata
     9661 aaaaaagaac agcgtaaatg ttttcgaaat gctttcgcac caacatttac ctgttccgtg
     9721 ggtttggcac ccaatgggcc gtgaggggct cgaacccgcg acccgctgat taagagtcag
     9781 ctgctctacc aactgagcta acggcccgta caaaaagtac ttaataatag tagcactaag
     9841 aattttaaaa tgcaagacta catttaaaga aaatcagaaa aatctttact tccttatagg
     9901 aaatcattca tagattattc acatttgctc gctattatat aagcatacca acaaacaaag
     9961 ttcttgcaag agaacaaacc ctaacacttt tttcattttt tactcctttc ccgccgtctc
    10021 cccggcgggt ctcctgttct taacgataaa agacaagaag gcttagcctt cttgtttttt
    10081 gttttcatgg aatcccgcta atcaaatcac ctagttaaag caactagatt gaatgtttat
    10141 cataagtctg atatcatgtt tatgaaatga aaaaaggagc ggttatgatg gaaaaaatgc
    10201 gtcagcaatt aatggagtgg gggaaggaac tagaaacatt ccgtctgcct cactgggaag
    10261 aactccctga cttagaactt tatatggacc aagtcagaac gttagttgat cgttatcttt
    10321 cacctgtgat acaaggagaa aagcatccat tgcttacctc ttctatggta aacaattacg
    10381 ttaaactagg tttgatacca gcaccagtta aaaaaagata taacaaagaa cacgtagcat
    10441 ttttacttgc aatcactacg ttgaaacaag tattgactat tcctgaaatc aaagaaggga
    10501 tattgttcca aggaaaaaca gtaggaattc gtgaagccta taatctattt tgcgatgaac
    10561 aagaagcagc tgtgtggatg gtcagccaat tagcacaagg taaatctcat ccgcaaaaat
    10621 tcgaccaggc aacccctgta gaatatattg ctgtaagagc ggcaacttta tcgtttgcaa
    10681 tgaaacttct tgcggaaaaa acgattgttc tagaaaccga atatttaaaa gaggagaaaa
    10741 caaatgaaaa acaataaaat cgcaatttta gtagattcag gtacggatgt tccagaagaa
    10801 ttgataaaaa aatacgagat gtatatgatc ccattgaaaa ttatttataa agatcaggta
    10861 tatacagata aaattgacat tacccctgaa gagatctatc aacgtttgcc agaggaaatc
    10921 cctggtacat ctttgccgga tggtgagtcg atcacagaaa tctttgagca aatcaaaaaa
    10981 gacggttatg aaaaggtact tgcggtaaca atttccagtg gattgagcgg gacatataat
    11041 gtggttcgtt taattgcaca acagcaagaa gaattggaag catatgtact agatacgaaa
    11101 aatattggta ttggtgcagg tttttctgcg atccaagcag caaaatggct ggaagaagga
    11161 gtagaatggg atcaactgat cagtaatctg aatgagttag tcgaaagaac aaaagttttc
    11221 tttaatgttg cgacattgga atatcttcaa aaaggtggac ggatcggttt agttgcttct
    11281 attgtaggaa ccgctttaaa gcttaaccca atcatctcat gtaatgaaga agggatttat
    11341 tacacagtag gaaaagctag gggacgtaaa aaaagtttgg ataaaacggt tgcttacgtc
    11401 aaagaaaaaa tcggtaatgc aaaggtcttc aacttagctg ttgcccatgg ggatgcgaaa
    11461 gaagaagcaa ttgaaatgga agcgagactt aaaagagagt tcccacaagc aaaccaaatc
    11521 tactttggcc agatttctcc ggcattagta gtccacactg gtccgggatt attgggtgtc
    11581 ggtgtacaag ttttagaagg ataaatcaaa aaaaggaaac actgaggtga aaatcagtgt
    11641 tttccttttt tattgaagtt agaacaaaaa ataataaagt attttgcact ttcattttaa
    11701 ttttctcttt tgttttatag gcaaagatga aacaaaagca gaaatacggt ataatgagaa
    11761 ggtatgacaa taatatataa gaggtggctc tttatgaaaa aagttttagt tgtcgatgat
    11821 gagaagccga tttcggatat cgtcaaattc aatttagcca aagaaggcta tgacgtttat
    11881 actgcatatg atggggaaga agccttggaa aaagttgctg aggtagaacc tgatttgatt
    11941 ttacttgatt tgatgctacc taaaatggac ggcttagaag ttgctcgtga ggtacgcaaa
    12001 acttatgata tgccgatcat tatggtaaca gccaaagatt ctgaaattga taaagtccta
    12061 ggcttagagt taggcgccga tgattacgtg accaagccat tttccaaccg agaattagtt
    12121 gcacgtgtca aagcaaatct tcgtcgcggt gccacggctg cgaaggaacc agaagaggca
    12181 gcacctgcgg aattgacgat tggtgatttg acgatccatc cagaagctta catggtgacg
    12241 aaacgaggag aaacaatcga gttaacacat cgtgaattcg aactactctt ttacttggcg
    12301 aagcaccttg gacaagtgat gacaagagaa catttattac aaacggtttg gggttatgat
    12361 tattttggag atgtacgaac agtggatgta actgtacgtc gtttgcgtga aaagatcgaa
    12421 gataatccaa gccatccaaa ttatttagtg acacgtcgtg gtgtcggtta ctatttgcgt
    12481 aacccagaac aggagtaatt ggatgaaaag aaaagtccgc ttttttcgat cagtcaattt
    12541 taagatcgct attaccttta ttctgatttt attgatctcg atcgaaatta ttggtgccta
    12601 ctttatacgt ggtttagaac gttccacgat caatacattt attaaagata tgaatcaaac
    12661 ggtggaatca ttagcaacaa caatcagccc ggaattgaat cgaaaagata atgcagatga
    12721 tgaagaggta aatgcgaata tcaaaagatt tattgaaaac agtgcgacta gcgatatcat
    12781 cgaaatacgg gtcgttgacg aaaaagggat catccgagga acaaccgatg tcaatgaaca
    12841 aagcgctgtg ggaaagaaaa atgattatgt tgatatcaat gatttcacga ctaaacgtta
    12901 tgttgcttta gataacgacg ataaacgggt gaatatcaat gttcaaccga tcctgtcccc
    12961 gaccggcgat gccgttatcg gggctcttta cgtcaaaagt aatatcgaac aaaaatattc
    13021 ggaaatcaat aacacagcag tcattttctt tactgcctca ctaattgcgg catttatttc
    13081 aatgatcgta tctgttttag ttgctcgttc gattacacag ccgatcggtg aaatgagaga
    13141 acaagccatt cgaatcgcta gaggagacta cagtcggaaa gtcaaagtgc atggacaaga
    13201 tgaacttgga cagctggctg ataccttcaa tcagttagct gaacgtattg aagaagctca
    13261 agatacaatg gaagctgaac gcaatcgttt ggatagcgtg ctatcccata tgacagacgg
    13321 cgtcattgcg acagatcgca ggggtaaagt cattacgatc aatgatatgg ctgtttcctt
    13381 gctagatatc aaaaacgagg aagcaatcgg acaatcgatt ttgacacttt tagatatcga
    13441 agaagaatat acgttgcgga aattattaga agatccaaac gaaatggtac ttgaccgttc
    13501 aaccagtaca ttggaaagtg atcagatgat cttgcgagta gacttttcta tgatccgtcg
    13561 cgaatcaggt tttatcagcg gattagtagc tgtcttgcat gatgtgacag aacaagaaaa
    13621 aacagaacgg gaacgtcgtg aattcgtctc taatgtttcc catgaacttc gtacaccgtt
    13681 gacaagtatg cgtagttata tcgaagcatt gagcgaaggt gcgtggaaag acgaggaagt
    13741 agcaccaaat tttttgaagg taacgctaga tgaaacagac cgaatgattc gaatgatcaa
    13801 tgacctctta aatctatctc gcatggatac aggtaatacc cagcttcaat tagaatatgt
    13861 caatttcaac gagttagtga attttgttct tgaccggttt gatatgatgg tgggaaatca
    13921 ggaaaaaaat tataagatcc gtcgtgaatt tacgcaaaga gatctttggg tcgaagtaga
    13981 tacagataaa atcatccaag tagtcgataa tatcatgaat aacgcgatca agtattcacc
    14041 agatggggga acgattactt gtcgcttatt agaaacgcat aataacgtga ttttaagtat
    14101 caccgatcaa ggattgggga ttcctaaaaa agatctgaac cgagtgttcg agcggttcta
    14161 tcgagtagat aaagcacgtg caagagcaca aggtgggaca ggacttggat tagctatttc
    14221 tagagaagtg attaaggcgc atcgaggcgc tatttgggca gagagcaagg aaggaaaagg
    14281 gtcgactttt tatatctcgc tgccatatga accttatgag gaggaatggt gggaatgaga
    14341 atctctgaaa aagtcgttcg tataggactc attttgatgg ttgcgttaag catatacttc
    14401 tcttatgcaa tctggctgag cccagcagga aaaagttcaa tcaacgtaga tgagacaaac
    14461 actcaaatga tcgagtctca aagcaatcga aaaacttccg aagtctttct tccgcttcat
    14521 gttacatggc accattcggg aatgatccaa gaaaccaaca gtgaaaatct cgtgtaccga
    14581 ttgcaattga tcatcgataa agcaagattt ggaaagcttt ctgaagtagt ttcaggagac
    14641 aaatcacagt ttgaacaggt gaaaaatctg aactctggtt ttgagttggc ctataatgcg
    14701 ccttttttgc taagagaata caaggaagca tttgatttag atttagatgt gtcgaacttg
    14761 gcaaaagaaa gcgaaaatat gtattttact agaatacaat tcgatcaagg aaataaaaaa
    14821 atccgatttt tgaattattc gaaccatctc gtatatgaag caaatatttc gttgaattgg
    14881 acagatatca ataaagaatt aacagcaaaa gacacaaaat ggacagaaat gacaacagaa
    14941 ccatcgacga tcgacacgca atatgctacg aaacattcga tcaaactgaa gaagtatagt
    15001 tatatcctgt cacagcaacc ctatacactt tttagaaatg cttttttcca gaaaccagat
    15061 agcgtgaaaa ataatgatga aagcaatgaa ttgatttttt acggtgggaa tgaaacaatg
    15121 tctatccatt cggaaacgca agtagttgat tttcgtggag agctgccaga gaaaaaagaa
    15181 aatgctagtg tattttctga aagtttcccc tatattagca aattaggcag ttctttaggg
    15241 aacctcagat attttgaccg cagtgacaat acgatcgatt atcggatatt cgtggaaggc
    15301 ttcccggttt ttgggacaga cagcagagga cagattggtg tggaagttgg cggtgaaacg
    15361 aatggttctg tgcaagtgaa tatccaaacc agtttgaata cgattcaagt gccgatacct
    15421 tcagacgaag aagtagaact tgcaagtacg aatgaaatca tccagcagtt aaaagctaag
    15481 ggagcagata gcaacaagat tggttcagtg atcgttggct atacatggca aaatgtcaaa
    15541 gaaacgaatc gtgtcgttga tctcttacct gaatggtatg tgaaatataa agataactgg
    15601 tattcagcta acgaccttct ggaaaaatta cctggattgg aggcgaatta attggatttc
    15661 aaaaaaatcg aatggatatt tttcgtcgct tttttagggt tgaatgtgtt tttattcagc
    15721 agttatcatg acgcggtata ccaagaaaac aatgtcattc gctctaatca gactgaatca
    15781 atccaacaac gtctggctag cgacgatatc acttacacgg ggaagatttc gtctgagaac
    15841 aagcaaggct attatcttag cgcggagcag accaatctta gcacttcatt agcgaattat
    15901 cgcaaggctg gtaaaagcgg cttgctggca agtggtgtgt ttttagacgg tgatgttttg
    15961 acgaaatctt tatccagttc agatgctgaa aagaacgtga tcgatccaga taaaattagt
    16021 agttccttgg aagatttttt atctaacaaa gacaatattt tattcggtaa tgattatgtg
    16081 tacacgaaaa acttttctca tttagaagaa gaaccactag aaatccaagc aagccaagag
    16141 tatgaaggca tcccattcaa tgatgataca gcaaaaatcg ttctttcttt agaaaaaaac
    16201 gataatgatt atagcatcac gaaatacatg cagacgcatt tatctgatat cgagcagctg
    16261 cgcgaaaaga cggaactgta tacggaagag gaagcaatca atacattgta catcaataac
    16321 aaaataccaa gaggatcaaa aattttatgg cgtcagctag cttattcctg tattctgaaa
    16381 gtccgggaaa aaaatgtgta tgttcctgtt tggcatgtag cgatcgaaac aagcgataaa
    16441 gtggtccaaa ttgagagcgt caacgctttc agcaatacga tcgtgacaaa caatactgta
    16501 ccaaaagtgg aagatcaata aaaaagcgag tataatgaaa aaagtttaca aagtaagaag
    16561 ggaagtagga gtcaaatggc tgaagaaaaa ccagctttta aaatcagtat cctagcaagt
    16621 ggaagttcgg gtaattcact ttatatcgaa agtgaaaaaa aacggctttt ggtcgatgca
    16681 ggacttagcg gtaaaaaaat cacttccttg atggctcaaa ttgatcggac accggaccaa
    16741 ttagatggaa tacttgtgac gcatgaacat cgagatcata tacatggcgt gggtgtcttg
    16801 gctcgtaagt atcatttgga tgtttttgcc aatcagcaga cttgggaagc gatggaacca
    16861 ctgattggaa atgtaccagt ggaacaaaaa catctatttg agatggggaa agtcagaaca
    16921 ttcggcgata tcgatattga gagtttcggc gtctcccatg acgcagcggc tccgcaattt
    16981 tatcgtttcc ataaagaaaa tcggtcattc gtcatgttga cggacaccgg atattgcagt
    17041 gattatatgc gaggtgtcat tgaaaatgcc gatggttatt tgatggaaag taatcatgat
    17101 cttgagatgt tacgcatggg accttatccg tggaatttaa aacagcgtat tttaggagat
    17161 agaggacatc tttccaacga agacggtgca cttgtcatga ctgatgtgat cggtaatcgg
    17221 accaaacgaa tctatcttgg ccatcttagc aaggaaaaca atatgaaaga acttgctcat
    17281 ttaacaatgg aaaatgtttt gaaagaaaaa gattttggcg ttggacatga ttttgaaatc
    17341 tatgatacag atccagatga agcaactgag ctattttcga tttaaacagt tcaatcaata
    17401 aaaatataaa aagcagaaga taaaatcaat cagggttggt tttatcttct atttttattt
    17461 ttttagcata ttaaaaaaat aaccgtatta tcgactgact acactgatgt gctgcttttc
    17521 atgtttctca tccagtatct catctactaa tgcaatcgcg taatctgcca tgctgatcgt
    17581 actgtcgcta gcagcgtttc tttgtaaata atcatcactt aacaaataag tacctgtacg
    17641 tttgccatgt ggatcaaata aagaagctgg actgatataa gtccaatgta gatttttaga
    17701 ttgtttcaat tttaacaatc cttcgtacat attgaaagct gttggataga agggtgcatt
    17761 tttaggtact tggtcaatca tacgagtcgt cttttctgta tctaagaata acgaagaggc
    17821 acccccaacg acaattaagc gtgtatcggt tccttcaagc atctcaatca aatgggacaa
    17881 agaagtttga tgcatttctt cttttccttt gggagcgttg aaagcatcaa cgacaacatc
    17941 aaaagatgca agatcagtag ttgtcaactc aaataaatcc ttttgtataa acggaacatc
    18001 tgatggcaat ttttctggat gacgcacgat tgccgtcacg tctaaatctc tggacaatgc
    18061 ttcgtctaaa ataaatgaac ctgcatggcc agtagctcca atgagtgcaa ttttcataaa
    18121 aaacctcctt ttttattcct gtccattgta agcagttgct gaatattcgt caaagaatcc
    18181 gatttttgaa aagctcagtg cgcattcctt tcatggttcg tgatcattgg gtatagttaa
    18241 attaagaaaa ctgattgata aaaatagaag ttgaaggaga taagaaatga aagagtggtt
    18301 agatcgtgcc tttttaccaa tgataacatc tgttgtgaat cttagaccct taactgcttt
    18361 gcgtaatgga atgacttaca cgatcccaat aattattgtt ggctcgctgt ttttcttatt
    18421 tgctcatttt cctgtggatt acatcgctca gtggatagac aaaacaggat tttctatgta
    18481 ttttacacaa gtataccagg ctacatttga tgtgatagcc ttttggatcg tttttgccat
    18541 tgcatacgct tatgcaaaag aagaagacat cgatcgctta ccagtcggca taacagcctt
    18601 gtctgcttat ctactgctta ttcagccact aaatactcag atagatactg tctggacagg
    18661 tgctgaaggg atactaggag cagttacaac tgggctcatc atcggatggg gctatgcacg
    18721 tttagtaaga cttgcgaaag ggtggaagca aagagagaag atacctgaaa atgtttggca
    18781 gtcttttact tcattgattc caatcattgt tatagtcact tttacgctta tcggttcagc
    18841 ggtttttcaa catttgttca gaatgactgt tgttcaagga gtatggacat atgtccagct
    18901 tcccattcaa tcgttcatag acacgttgtt tggtgtgctt ttattaggtt tttttgttcc
    18961 gtttttatgg ttatttggtc ttcatggatc tgcgatggtc aatgggttgg ttagtcccgt
    19021 cttgcaagcg aattcattag caaatgcaga aatcttagcc tcaggacaag agttgacggt
    19081 ggcaaatggc ggacatatcg ttactcagca atttctcgat cagttcatga cggttacggg
    19141 tgctggattg acgttagggg ctgtattttt tatgacggtt tttgcgaaat cgaagaaata
    19201 cagagaatta ggaaatcttg ctcttttacc agcctttttc aacatcaatg aatcaattat
    19261 tttttccaca ccgatcgtga tgaacccgat gatggctgtt ccttttattt tcgcaccggt
    19321 cgtttcagga ttgattacct attccgcttt atattttgga ttcgttcctt tatttacagc
    19381 agtccaagtc ccttggacaa caccgccgat cctctcgggt ttcttagtag gcggagtgcc
    19441 tgcagcgatt ttacaaggta tcgtgttgtc aattagtttt ttcatttatt tcccattttt
    19501 gaaaaaaata gattctgcaa actgcaaaaa ggaaagacag acaaaaaatg atggagcagc
    19561 agaaaagatg aacgactaaa aaacaaaaat actcctgtcg ctttagattg ggaaaatcac
    19621 caatctagga tgacaggagt atttatagtc atgtttatac tagccctttt tggacagcta
    19681 attgaacaag aacaactaat gtgttcattc ctacgtgtgt gatcatcgat gtccagatcc
    19741 gaccagttgc tttgtactct aggctgaaga agaaacctag gaaaaagtag attagcagat
    19801 gaccatcatt atgagcaaac gcaaacagca aagagctgat cactgctccg acccaaaaat
    19861 ttgaatatcg actgatgatc cccagtatac tgcggcggaa tacgaactct tccatgatgg
    19921 gtccagcaac agttgtcgcc aaaataaaag cgggtgcttc catgatcatc tggatgatat
    19981 tttgtgtatt ctcggaaggg gttgctttgc caaacaatac agattctaac atgacagcga
    20041 tgctttgcag aatcaatgcg acaaagattc caaccaatcc ataaataaca atctttggag
    20101 cagaagcttt ttttaccgct gattcgatcg ctaagggttc acgttgctta aaataaaaaa
    20161 tagctaggac aacggcgcct agtatataca tgactgcagt tgctgtgatc aattcagacg
    20221 tagattttac aagtcctatc gaagctagga tcaagggtga gataaaaatt agaccatagc
    20281 aaaaaatgct gaggaagcta tattttttta aagacatgta attcctcctt ttaagggata
    20341 acaagcatgc aaggcaagct tatatcgatt gatttcaatt atatcataat ttcaataagc
    20401 agctgtcaga ataaaatttc ttttctttga tttaaaagct tgcaaaaaag agggagaatg
    20461 agtattatta aaaatgtagg ttagcactcg aaggttaaga gtgctaacag attaaaaaaa
    20521 gagttatcgg agggatttat cgtgttaaaa ccattaggtg atcgcgtcat tatcgaagtc
    20581 gcaaaagaag aagaaaaaac tgtcggagga atcgttttag cttctgctgc taaagagaaa
    20641 ccccaaactg ggacagttgt tgcagtcggt gaaggccgtt tgttagaaaa cggtgaaaaa
    20701 gttcctgccg ctgtaaaagc aggcgaccaa gttatgttcg aaaaatacgc cggtacagaa
    20761 gtaaaatacg aaggaaaaga atatctgatc gtcgcaggaa aagatatcat ggcgattgtt
    20821 gagtaaattt ggaagataaa aaaataatca aaaataatca atgaggtgaa tcaatcatgg
    20881 caaaagaatt gaaatttgca gaagacgcac gcgcagctat gttacgcggg gtagataaat
    20941 tagcagatac agtgaaagta acattaggac ctaaaggccg aaatgtcgta ctagaaaaat
    21001 cttacggctc tccattgatc acaaatgatg gtgtgacgat cgcaaaagaa atcgaattag
    21061 aagatcattt tgaaaacatg ggtgcaaaac ttgtatcaga agtagcatca aaaacaaatg
    21121 atattgcagg agacggtaca acgacagcaa ctgttctgac acaagcaatc gttcgcgaag
    21181 gtttaaaaaa cgtaactgca ggtgctaatc ctctaggcat tcgtcgcggg atcgaattgg
    21241 caacaaaagc agcagtcgaa gaattgcata atatctcaac tgttgttgat tcaaaagaag
    21301 ccattgcaca agtagcagct gtttcttcgg gttctgataa agttggacat ttgattgctg
    21361 atgcaatgga aaaagttgga aacgacggtg tcatcacaat cgaagaatca aaagggatcg
    21421 aaacagaact agatgttgta gaaggtatgc agtttgatcg tggttactta tcacaatata
    21481 tggtaacaga taacgacaaa atggaagctg ttctagaaaa cccatatatc ttgatcacag
    21541 acaaaaaaat ctctaacatc caagatattc tgccattgtt agaacaaatc ttgcaacaat
    21601 cacgtccatt gttgattatt gctgacgatg tagatggtga agcattgcca actcttgtat
    21661 tgaacaaaat ccgtggaaca ttcaacgtag tagctgtaaa agctcctggt ttcggcgatc
    21721 gtcgtaaagc aatgcttgaa gatatcgcta ttttgacagg tggtacagta attactgacg
    21781 atctaggttt agaattgaaa gatgcaacaa tcgaaaactt aggaaatgct tctaaagttg
    21841 ttgtagataa agacaataca acgatcgtag aaggttctgg agaaaaagaa gcaatcgaag
    21901 ctcgtgtgca attgatcaaa aaccaaattg ctgaaacaac ttctgatttt gaccgcgaaa
    21961 aattacaaga acgcttagct aaattagcag gtggtgtagc tgttgttaaa gttggtgcag
    22021 ctactgaaac agaattgaaa gaattaaaat tacgcattga agatgcattg aatgctacac
    22081 gtgccgcagt agaagaagga atggtctcag gtggtggtac agctctagtg aacgttatca
    22141 gcaaagtttc tgctgtagaa gcagaaggag acgtggcaac aggtatcaag atcgttgttc
    22201 gtgctttaga agaaccaatt cgtcaaatcg ctgaaaacgc tggttatgaa ggatcagtga
    22261 ttgttgataa attgaagaac gtggaactag gcactggttt caatgctgct actggagaat
    22321 gggtaaacat ggttgaagca ggtatcgtag acccaactaa agtaacacgt tctgccttgc
    22381 aaaatgccgc ttccgtttct gccttgttat taacgacaga agcagtcgtt gcagataaac
    22441 cagaaccagc agcgccagca gctcctgcaa tggatccatc aatgatgggc ggtatgatgt
    22501 aagaaaaagc aacttgctta tctacaactg atcattagtg aaagacagat aaaaagaggc
    22561 tggaacagaa gcgtttaact ccaagaaata agaagaaatt cacggaaatt gcttttcaaa
    22621 tttttgtgaa tttcagctta tttccgaagg agttgcttct gcttccgctg tttatacgtg
    22681 tttaga
//
LOCUS       DACCWK010000036        22623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCWK010000036 DACCWK010000000
VERSION     DACCWK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.036"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            3..815
                     /locus_tag="IVW65_001344"
     CDS             3..815
                     /locus_tag="IVW65_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7900229.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="IVW65_001345"
     CDS             812..1612
                     /locus_tag="IVW65_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7900230.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="IVW65_001346"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="IVW65_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7900231.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSRK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="IVW65_001347"
     CDS             3109..4818
                     /locus_tag="IVW65_001347"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7900232.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9382
                     /locus_tag="IVW65_001348"
     CDS             5030..9382
                     /locus_tag="IVW65_001348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7900233.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9526..9999
                     /gene="rimP"
                     /locus_tag="IVW65_001349"
     CDS             9526..9999
                     /gene="rimP"
                     /locus_tag="IVW65_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7900234.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10023..11198
                     /gene="nusA"
                     /locus_tag="IVW65_001350"
     CDS             10023..11198
                     /gene="nusA"
                     /locus_tag="IVW65_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7900235.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11220..11513
                     /locus_tag="IVW65_001351"
     CDS             11220..11513
                     /locus_tag="IVW65_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7900236.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11510..11821
                     /locus_tag="IVW65_001352"
     CDS             11510..11821
                     /locus_tag="IVW65_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7900237.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11834..14140
                     /gene="infB"
                     /locus_tag="IVW65_001353"
     CDS             11834..14140
                     /gene="infB"
                     /locus_tag="IVW65_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7900238.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14167..14514
                     /gene="rbfA"
                     /locus_tag="IVW65_001354"
     CDS             14167..14514
                     /gene="rbfA"
                     /locus_tag="IVW65_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7900239.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14622..14843)
                     /locus_tag="IVW65_001355"
     CDS             complement(14622..14843)
                     /locus_tag="IVW65_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7900240.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15291..17653
                     /locus_tag="IVW65_001356"
                     /pseudo
     CDS             15291..17653
                     /locus_tag="IVW65_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18021..18944
                     /gene="truB"
                     /locus_tag="IVW65_001357"
     CDS             18021..18944
                     /gene="truB"
                     /locus_tag="IVW65_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7900241.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18948..19889
                     /gene="ribF"
                     /locus_tag="IVW65_001358"
     CDS             18948..19889
                     /gene="ribF"
                     /locus_tag="IVW65_001358"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7900242.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20241..21026
                     /locus_tag="IVW65_001359"
     CDS             20241..21026
                     /locus_tag="IVW65_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7900243.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21039..21890
                     /locus_tag="IVW65_001360"
     CDS             21039..21890
                     /locus_tag="IVW65_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7900244.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22014..22466)
                     /locus_tag="IVW65_001361"
     CDS             complement(22014..22466)
                     /locus_tag="IVW65_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7900245.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7677 a         3952 c         4948 g         6046 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaggaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccaaaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5701 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5761 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5821 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     5881 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     5941 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6001 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6061 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6121 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6181 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6241 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6301 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6361 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6421 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6481 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6541 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6601 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6661 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6721 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6781 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6841 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     6901 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     6961 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7021 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7081 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7141 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7201 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7261 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7321 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7381 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7441 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7501 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7561 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7621 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7681 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7741 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7801 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7861 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     7921 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     7981 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8041 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8101 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8161 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8221 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8281 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8341 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8401 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8461 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8521 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8581 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8641 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8701 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8761 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8821 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     8881 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     8941 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9001 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9061 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9121 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9181 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9241 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9301 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9361 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9421 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9481 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9541 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9601 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9661 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9721 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9781 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9841 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
     9901 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
     9961 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10021 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10081 aagaaatcgt gatcgatgct ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10141 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10201 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10261 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10321 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10381 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10441 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10501 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10561 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10621 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10681 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10741 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10801 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10861 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    10921 caaatccaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    10981 aacgcggaca aaacgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11041 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11101 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11161 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11221 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11281 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11341 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11401 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11461 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11521 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11581 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11641 aaccgatgca agcgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11701 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11761 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11821 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    11881 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    11941 tgcgattact agcaatgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12001 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12061 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12121 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12181 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12241 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12301 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12361 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12421 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12481 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12541 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12601 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tcgtaacgat
    12661 tatgggacac gtagaccatg ggaaaacaac tttattggat acattacgcc attcacgtgt
    12721 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12781 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12841 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    12901 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    12961 tgtaaataaa attgataaac caggtgcaaa ccctcaacat gttatgcaag aattaagtga
    13021 gtatgagtta attcctgaag catggggtgg agaaacgatc tttgttgaga tctcagcgaa
    13081 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13141 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13201 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcatg ttggtgaccc
    13261 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13321 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13381 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13441 acgcgcgaaa cgtgcattat tagaaaaccg tgctgccaca agccgtgtga ctttggataa
    13501 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13561 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13621 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13681 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13741 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13801 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13861 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    13921 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    13981 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14041 aatgggttat gaatgtggtg caatgattga aaaattcaat gacatcaaag tagatgacgt
    14101 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14161 atcattatgg ctaactaccg tgaccgccga gtcggtcaag aaatcttaaa agaagtcaat
    14221 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14281 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14341 tcagataaga aaacagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14401 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14461 gttgtctatg gcaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14521 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14581 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14641 agaaaagtaa aaaattcgac tttctttttt ctatagtata taaatttttg catatagata
    14701 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14761 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    14821 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    14881 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    14941 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15001 gaattgctcg atccagcccc agttttccaa caatgtgtta actggcctag cgtcattcag
    15061 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15121 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15181 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15241 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15301 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15361 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa ccagaagatg
    15421 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15481 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatattgaa gggccaggac
    15541 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15601 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caaacgattt tcatttcctg
    15661 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15721 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15781 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    15841 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    15901 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    15961 ttaacggact tggctgggaa cctttcttta ttgaaggaaa tgattcagaa aaattaaatc
    16021 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat taaatccatc caaaaagaag
    16081 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16141 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16201 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16261 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16321 aagaaatcag agaaatcgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16381 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16441 ttgaaaaacc aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16501 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16561 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16621 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16681 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt ttgcaagtta
    16741 cgaatcattc ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatgcggaa
    16801 atcacatgac ctttcttggc gaaatgatta tccttcgcta aatctcattg caagctcaac
    16861 agtcttccag caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt
    16921 ggcagaaaag aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct
    16981 cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa
    17041 acatccgcgt gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt
    17101 gaaaatcatt gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc
    17161 tgcaggaacg gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt
    17221 cccaaaacta aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag
    17281 tcaagatccg cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc
    17341 gatcttattt gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca
    17401 taaccatcat ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga
    17461 catgcgtgta gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat
    17521 caaaggaagt caagcagaag attttgcgaa aaaaatggat cagaaattac aagaacatca
    17581 aaactatatt cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa
    17641 tcttgaccaa taaaatgata aaatcgatcc cgcaaaatag aattttttac attgattaag
    17701 aaactgaaaa aataaataaa aaaaacggaa aaaagactgg aagaatggcc agtctttttt
    17761 ttcgtttttg ggaagtgaaa accaaactat ttacctgtaa gccacccata aatccaatca
    17821 aatggtgcaa agaaaaaatc ttccattcta ttccctcctt taaaaaagca taacatgatt
    17881 tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa aaaccgttca
    17941 ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca gttaaatcaa
    18001 aaatgaaatg gagaaaagac atggacgggt tattaccttt atggaaagaa cgcggaatga
    18061 caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa atcggacatg
    18121 ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga aaagccacaa
    18181 aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata actctaggtt
    18241 tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca gtagatatgt
    18301 ctttatctga aaaaaaaata gatgaagtga tggcttcatt tattggtgag atcacccaga
    18361 taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa tatgcccgca
    18421 ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt atccggacaa
    18481 gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg
    18541 ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt ggttaccctg
    18601 cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaag gatgctctga
    18661 cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa
    18721 tagaaaaagg agtggaacgg ttcaaacgag tagatattac tgatgaagta tggcagaaag
    18781 taaaaaacgg tatgagactt gattatactg tatttgcgct cccagaaatg ccgtcagaag
    18841 aaattgcttt gttttataaa ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa
    18901 aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg aatatcatca
    18961 agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg
    19021 gatttttcga cggcgtgcat atcggacacc agaaagtcat aaagacaggt atggaaattg
    19081 cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca tctattgtct
    19141 ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa aaagaagaaa
    19201 aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct tcttttgctc
    19261 atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca aaatatgctg
    19321 tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct cattttcctg
    19381 aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa
    19441 aaatcagctc tacccgtatt cgtggattac tagacagcgg acaagtaaaa gaagcagcaa
    19501 aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat gcacgaggca
    19561 gactactagg attccctaca gcaaatgtca aagtaaagag cacggttcat ttgcctaaag
    19621 aaggtgttta tgtgtcggaa ataaaaattg gggaaacatg gtatccagcc atgggttcaa
    19681 ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagagttg tatattttgg
    19741 actttaatca agatatctat ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag
    19801 atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaggag gatgaacaag
    19861 caacagctga atatttttct cagcaataaa acattatgca taaaatctct tgtttatgta
    19921 taactagcaa ttacaataaa atgggtgata aataagccca ggaaaggggc tgtgacaaaa
    19981 gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt cttcggaaat
    20041 aagccgaaat ttcttgaagc tgaccacttc tgtcacaacc tctttcttta tagaaataaa
    20101 aggacactag caattcatga aaacgattgc tagagcgtat catttgcttt agactatata
    20161 tcttggtgta cactcacttt aaggcggaaa taaaagttat ccgtaataac aggtttaggt
    20221 atgaaaaagg agtgggtact atgtacaagg aattagaaaa caaagtcgca gtagtaactg
    20281 gtggctcaaa ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg
    20341 tagttgtcaa ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag
    20401 aggcaggcgg aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc
    20461 gtttacttga tacggcttta gaaaattttg ggacactgga cgtttgggtt aataatgctg
    20521 ggatggaaaa tcagcatcca acccatgaac ttagccttga agattgggaa agagtgttaa
    20581 aagtcaattt gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga
    20641 ataataaaaa aggaaatatc atcaatatgt cttctgttca tgaacagatc ccatggccaa
    20701 cgtttgctca ttacgctgct tcaaaaggcg gggtaaaatt atttaccgaa acggtagcaa
    20761 tggagtatgc accgaaggga atcagaatca atagtatagg ccctggtgct atccgtacac
    20821 ctattaatgc ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc
    20881 cactaggacg cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag
    20941 acgaatccag ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc
    21001 cttctttcca aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga
    21061 tcccggcagt tggctggggg atccagccac tgattttgaa aaagattggt ggcagaccaa
    21121 caaatgagat attaggaact ggtattggag cggtgcttgt cggcttactc gttcaattat
    21181 tcgcttctcc agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat
    21241 tttgggttat tggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa
    21301 cgatgccaat ttcgactgca ttacagttag tcggaacctc gttgattggt gtcttcgctt
    21361 ttggagaatg gtctggaagt atggggaaaa tcattggagc agcagcaatc attcttcttg
    21421 tatttggttc tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa
    21481 aaggaattag tctattagca tcaacaagta ttggttattg ggtatacagt gcattgccta
    21541 aactagttga tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag
    21601 gggcagtgat ttatgtgatt ttccgtaaac caaccgcatt ttctgaagac aagagttgga
    21661 aagcaacgat cgttggagcc atctttagtg tgtcagcatt ggcttatatt ttttcagcaa
    21721 atgaaaatgg ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat
    21781 taggcggtat gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tga
//
LOCUS       DACCWK010000037        21723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCWK010000037 DACCWK010000000
VERSION     DACCWK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.037"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            54..1013
                     /locus_tag="IVW65_001362"
     CDS             54..1013
                     /locus_tag="IVW65_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7900246.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1180..2886
                     /locus_tag="IVW65_001363"
     CDS             1180..2886
                     /locus_tag="IVW65_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7900247.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(3044..3946)
                     /locus_tag="IVW65_001364"
     CDS             complement(3044..3946)
                     /locus_tag="IVW65_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7900248.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(3943..4647)
                     /locus_tag="IVW65_001365"
     CDS             complement(3943..4647)
                     /locus_tag="IVW65_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7900249.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(4678..5319)
                     /locus_tag="IVW65_001366"
     CDS             complement(4678..5319)
                     /locus_tag="IVW65_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7900250.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(5357..5671)
                     /locus_tag="IVW65_001367"
     CDS             complement(5357..5671)
                     /locus_tag="IVW65_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900251.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(5659..6288)
                     /locus_tag="IVW65_001368"
     CDS             complement(5659..6288)
                     /locus_tag="IVW65_001368"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7900252.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(6295..7011)
                     /gene="pyrF"
                     /locus_tag="IVW65_001369"
     CDS             complement(6295..7011)
                     /gene="pyrF"
                     /locus_tag="IVW65_001369"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7900253.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(7008..7940)
                     /locus_tag="IVW65_001370"
     CDS             complement(7008..7940)
                     /locus_tag="IVW65_001370"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7900254.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(7937..8713)
                     /locus_tag="IVW65_001371"
     CDS             complement(7937..8713)
                     /locus_tag="IVW65_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7900255.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(8767..11952)
                     /gene="carB"
                     /locus_tag="IVW65_001372"
     CDS             complement(8767..11952)
                     /gene="carB"
                     /locus_tag="IVW65_001372"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7900256.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(11957..13033)
                     /gene="carA"
                     /locus_tag="IVW65_001373"
     CDS             complement(11957..13033)
                     /gene="carA"
                     /locus_tag="IVW65_001373"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7900257.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(13038..14318)
                     /locus_tag="IVW65_001374"
     CDS             complement(13038..14318)
                     /locus_tag="IVW65_001374"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7900258.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(14337..15263)
                     /locus_tag="IVW65_001375"
     CDS             complement(14337..15263)
                     /locus_tag="IVW65_001375"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7900259.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(15276..16559)
                     /locus_tag="IVW65_001376"
     CDS             complement(15276..16559)
                     /locus_tag="IVW65_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7900260.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(16573..17112)
                     /gene="pyrR"
                     /locus_tag="IVW65_001377"
     CDS             complement(16573..17112)
                     /gene="pyrR"
                     /locus_tag="IVW65_001377"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7900261.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(17347..18258)
                     /locus_tag="IVW65_001378"
     CDS             complement(17347..18258)
                     /locus_tag="IVW65_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7900262.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(18255..18719)
                     /locus_tag="IVW65_001379"
     CDS             complement(18255..18719)
                     /locus_tag="IVW65_001379"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7900263.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(18724..19422)
                     /locus_tag="IVW65_001380"
     CDS             complement(18724..19422)
                     /locus_tag="IVW65_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7900264.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(19409..19795)
                     /locus_tag="IVW65_001381"
     CDS             complement(19409..19795)
                     /locus_tag="IVW65_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900265.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(19942..21612)
                     /locus_tag="IVW65_001382"
     CDS             complement(19942..21612)
                     /locus_tag="IVW65_001382"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7900266.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         6131 a         4870 c         3851 g         6871 t
ORIGIN      
        1 ttgtgctaca ctcaaaatag ttccgacgaa agaattataa ggagtgagta caaatgacct
       61 ataaccatct tacaccaact gaactcgtaa tgatagaagc ttatttcaat caatcacaac
      121 ctgtttcaaa agtagccaat ctgttacaac gttctcgaca aactatttat aaggtctatc
      181 gttttcttaa atctggtgga agcgcaatga gctactataa acaatataag aaaaacaaac
      241 gtaactgcgg aagacatccg attgttttac ctgatgagca acaggaatac attcaaaaaa
      301 aggttgttca agggtgggct cctgatgtcc ttattggtcg agcagagttt cctattgctt
      361 gttcggtacg cacactgtat cgaatgttta aagataaaac ctttgacact cacgatttac
      421 caatgaaggg aaaaagaaaa ccaaacggtc ataaagaaaa aagaggaaaa caagggtttc
      481 gccggtcaat ccgagacaga aagacagact atacccagtt tgatcatgaa tttggtcacc
      541 tggaaggtga cactatcgtg ggtggaaaac ataaaagcgc ggtcatcaca ttagttgaac
      601 ggctatctaa agtcatcatt accttgaaac ccgaaggcag acaggctaaa gacattgaaa
      661 ataggctgaa tcaatggttt cgttgtgtcc caaaaaactt atttaaatcc atcacatttg
      721 attgtggaaa agaattctca aattggaaaa agattagtaa taccaacgat atttcaattt
      781 attttgcgga tccaggaaca ccttctcaac gaggattgaa tgaacattca aatggcttat
      841 tacgtaaaga tggtttgtat aaagctatgg attttaatgg tgtatctgaa acctttatcc
      901 aatcggtggc atcgaaacga aatcatattc ctagaaaatc attacactat cggacaccaa
      961 tggaagtatt tttgagttac gtcacaaaag atgatttgtc taacttaatt tgacaaacga
     1021 aaatacctaa aaatttggat ttcaacgata gaaacactgt tttttatgta attagtccaa
     1081 gatttttcta gacttttgct ttactattcg gccaaattgg tttttcttct ttttttcgtt
     1141 ataatgaata tacttatgaa aagaaatgag gaatgaacca tgtcctttga tggtgtattt
     1201 acccacgcga tggtcaacga attgcgtgaa acgttactat ctggccggat ctcgaaaatc
     1261 catcagcctt atgaaaatga aatcgtgctg gtgattcgtt cacgcggcaa aaaccatcga
     1321 ttattattat ctgcgcaccc aagttatgct cgtgtacaga ttacccaaat cgattatcag
     1381 aatcctgata atccgcctaa cttcgtgatg atgcttcgaa aatatttgga tggcgctatt
     1441 ttagaagaca tcgaacaaat cgaaaatgat cgagtgatcc atttccactt tgctaaacga
     1501 aacgagttgg gtgatctaca aaatatcata ttgatcgttg aattaatggg acgtcatagt
     1561 acgatcgtct tagttaatcg agaaactgga aaaatactgg atgcgatcaa acacattgga
     1621 agctctcaaa acacttatcg ttctttgctt cctggtgtag aatatgttgc tccgccaaaa
     1681 caagaggtct tgaacccatt tagctccgag aaagaaaaaa tctttcagcg gctttcccaa
     1741 actgaactag atccaaaagc aatccaacgt cagtttcagg gaatcggttt cgatatcgct
     1801 caagagctga ccaaaagatt actggaacgt cctaatgaaa agatggttgt atgggacgaa
     1861 tttttctcag ccatcagcca tcagcctatt cctacttttt acgaaacgga aaataaagat
     1921 tttttcacac cgattgctta tcaggtattg tccgaacagg catctgctgt aacagcttat
     1981 ccaacactca gtcaattatt agatagctat tatcacgaaa aagcagagaa agatcgggca
     2041 aaacaacaag gcggagaact gattcgaaaa atcgaaaatg aactaaaacg aaataaaaat
     2101 aaattgaaaa aacgtgaaca aacactcaaa gaatccgaaa acgctgagaa ttaccgtaga
     2161 aatggcgaac tacttactac ttttctgaca caagtaccac gcggcgcaaa agaagttgtt
     2221 ttacctaatt attatgaaga agatcggcca atcaaaatag cactagatcc ggcattgaca
     2281 ccaaatcaaa atgcacagaa atatttccat cgataccaaa agctgaagaa cgctgttaag
     2341 ctgattggcg aacaaatcca agaagccaaa gatgaaatcc aatatcttga atctgtctta
     2401 tctcaactgg aaattgccgg cccaatggat atcgaagcaa ttaaagaaga attgacttca
     2461 gaaggatacc tgaaaaagaa gaacttaaaa aaacaaaaac gtaagaaacc atctcagcct
     2521 gatcaatact tttcttctga cggaacgctc attcttgttg gtaaaaacaa tcttcaaaat
     2581 gaccagctga caatgaagac agctaaaaaa accgactatt ggcttcatgc aaaaaatata
     2641 cccggttcgc acgtcatcat taaaagtgac aagccctcag atgaaacaat tacagaagct
     2701 gccgagttag ctgcctattt ttcaaaatac cgatactctg cccaagtgcc ggttgatctt
     2761 gtacaggtaa agcatatccg aaaaccaaat ggcgccaagc ctggttatgt tatttatgaa
     2821 aaccaaaaga cgattatcgt gacaccagaa gaagaaaaaa ttacagctat gaaacaaaac
     2881 aggtaatcaa tgagtgtttc tagaatcata tttgcgtact ttcccacaac agcaattagt
     2941 tataaattgt ttgctacaaa aagaggagtt gtgacaaaat tctgtcacaa cccctctttt
     3001 ttgatcaacg gtgttcaata aacagcgttc tcatctgctt ttcttagctc tgctggtcca
     3061 gtaaccgagt aagctctttg ataaatcttt gagcgatttc actgagcgga cgtttcgccg
     3121 aataaatgat ataaaattca tttagttcgc cttctgccaa agggattaac cgcaagtgct
     3181 cgcgaatccc tccaatcaca ataccagatc ctgaagcgta agcatcagtt tcaagaacga
     3241 tattcatcaa agtacctcga tcactcgtat aaatcactgt ttcttgatca gggatctcga
     3301 taaggtcttc ataaaaatac gggaaattat ttccatcctg tgtaaaacga acttgaggat
     3361 aagataacaa ttcttctttg gtgatctctg cgtgttcagc taatggatgt ccttgcctta
     3421 aaaaaatatg cgttttgaat tttccaatgc tgcggccact tagctctcct tgttgtaaat
     3481 atcttctgat accagcttga ttatgctcat tcatataaat aatgccgatc tcactgtgct
     3541 gctctttgac ttcttcgatc actctcgctg ttgtcgtctc catgatccga aattgtttga
     3601 tctcgtcttt gaattcctcg agtagctgtg cggtcaaagg acctaagaaa tcataatgct
     3661 gggcagcgat cgaaaaattg tctctctttt cactcgttag atagtgagtt tctaatgtat
     3721 caagctggac caatattttc tcggcacgat ttaagaagtc ttgcccttct tctgttaaat
     3781 atgcccctcg attcgttcga gtaaacagcg tgatccctaa ctcggtttct aattctttga
     3841 taccagctga aagacttggc tgggagacaa acattttctt tgccgcttct ctgaaactac
     3901 cattgtttgc tacagcgacc acgtatttca tctgctgaat attcattgct caaactcctc
     3961 gaaataataa atttctcttc cattcaaggg ctggattggt cggttgttcg gtcgagcata
     4021 ctgaccatcg aaggggagaa gaaactcttg gttcagtttt cctacatgat ccattacaaa
     4081 ccattggatt ggcccgctgg taggcggagt tgtcaatgag ccaatataat ggaaatggct
     4141 tttttgctcc ggaaagaaca catccggatt tacccaatgc tcatgttttt ctaagttcca
     4201 actttcccca ttttcttgat tatacagcca accttcctgt accattagct ggtacatgac
     4261 acccacaact agattctctt ttttgctgtt catatgaaca aaatgaaatt caatatcttc
     4321 ctgttttccg ttgatgatat gttcagaagg caagtggaaa tgaatatctg tcaaataata
     4381 gcgttctttc ttgaactcta cataactgag ttggtcaaaa ggtactaaat gaatcgtgtt
     4441 tttgaattcc ttgtcagtaa accgttgctt ttgataatga aaggaaagcg tgtctccttc
     4501 tattttggtt tcttccccct tggtcaaatg aataggcgac tgcaatggaa attccgcacc
     4561 tctcgcaaac caatcacata aactcgccca attttccgga cctttgtctc cagaatagcc
     4621 ccattcgaca tccatattct ttttcaacta tacttccccc tgcatctcat atcattttta
     4681 tttttcttct ttctttacaa tcgttgagac ggtttctaaa aacgcctctt ttccataaaa
     4741 ttcctggttc aatagttccg gaaatatctc tttaaagaaa tactgaacag aaggccgatg
     4801 ttcaaagctt ttaatcgtag tcactgcctc aatgaacatc tccttataaa ctttttccgg
     4861 gttatttttg atgatttcat caaaagattc ataaagaagg tcaaccttat ctgaaatggc
     4921 taatagttct ccttctacgg tttcatcttt tccttcgaac aaccgacggc gataaacttc
     4981 ttggaactca gctggaatct cttggttgat gaattcgtcc gtcatctttt cttcaactgt
     5041 ttgcagcatc cctcttaact ctttgcttgc gtatttcacc ggcgttttga tatcaccaat
     5101 aaaacgctct gtgtagtcat gattcaatga tttttcatag agcagtttcc aatcgatcgg
     5161 ttgtccattc gcttcttcaa tgtctcctag aacttgcgca atagatgcta cacgatacga
     5221 atgtgctgca actgtatgct ctgtatattt aaaaaatcca ggtgcccttg tgatcgtctc
     5281 taaattgttc aatcctaaaa taaattcatt caaccccata aaagttacct ccctttgaga
     5341 tttctttttc cgaatatcat atcattaaat aactgatatt cgcaacattt ccgctcttta
     5401 ccatttttga aacagctccc ttaccaaaaa atagagaaaa tctatatata atagaaaaaa
     5461 gagaagagat tattctcttc tttctgctaa ccagtcttag aaaaatgaat tttaaaaaaa
     5521 tcaaaaactg atcacgatag aagtttcttt tcatacctga tgaaaagatg cttctcgcaa
     5581 tcagtttttg gatttttgct attccaattg atgattctgg agacacaaat cggatttctc
     5641 tatcttcatc aagcgcgctt aaatctgcca attttcagga tcttttttcc attcttttag
     5701 cagagttaat tcttgctcat cgatataacg atcttctaaa gctgcttcaa tcaatacaga
     5761 ataattggtc aacgtcataa gagggatttc ggctttttcg aaatttgctt ttccttttgg
     5821 caactcatac gtaaagatag ccgcaacacc aaggatatcc gcaccttctc tttttgctgc
     5881 ttcagctgct tccaagacac ttccgcctgt agaaatcaaa tcctcgatca caaccatctt
     5941 ctgaccttca aatatacgtc cttcgatttg gttacctttc ccatgatctt tcgccttact
     6001 tcgaatatag accattggca aatccagtat ttccgctacc catgcggcat gagggattcc
     6061 tgctgttgca gttccagcaa tcacttgtac ttcagggtaa gcttctttga ttttgcttgc
     6121 taatcctttt gcgatctctt ttcggacttt tggatagctc atcgtgattc ggttatcaca
     6181 gtaaattggg cttttgattc cacttgccca tgtaaaagga ttacttggat ttaaaaatac
     6241 tgcttcaatt tctaataagt cttttgcgat actttgttca acattcattt taggttagct
     6301 ccattctgtt tgaatcgttt gatatgcttt ataaggatta gttgactgag tgattggtcg
     6361 acctataacg ataaaagttg agccgatctt ttttgcctga gaaggtgtga ctactcttgt
     6421 ctgatctcct gcgactgttc cttctggtct tatacccgga gttaagcaaa caaattcgtc
     6481 tcctgccatt tctttgatcg cttctacttc ccaagcagaa gaaaccacac catctaatcc
     6541 tgcttttttg gcacagctag cataatggat cacacttttt tctaaagaaa cttcgattag
     6601 ttgatcggca tgcatttctt cttcacttgt agaagtaagc tgagtgatag ctagaagtag
     6661 cggacgtttt ccaccttctg gagtcccctc ttctaatcct cgcattgccg cctccatcat
     6721 acgaataccg ccagcggcat gaacacaggt gagatcaaca ccaagctttg ccaatccccg
     6781 cattgctttt tcgaccgtat tcggaatatc gtgaagtttc agatctaaaa atacattatg
     6841 accagcctct tttagataac gtacgatttc aggtccttct tggtagaaca attccattcc
     6901 gattttcaca aacaattttt cttctttcgg gaaaaatcga agaaagtctt ctacctcttg
     6961 cctagttgaa aaatctaatg caataattgg tcgttggtct agttgattca tcgttcacgt
     7021 tcctctctca cttctttgat taatctttcc agcgattcga tgcctagttc gctcattcgc
     7081 acagggagct ctttaattag tttaggacag atatacggat cggtgaaatt cgcggtccct
     7141 atagctacag cacttgctcc agccataaac atctcaagaa catcatccac tgtctgcacg
     7201 ccgcccatcc caatgattgg aaggctggat atacttgcta cttggtgaat caaacgaata
     7261 gcgacaggct tgattgctgg tccggagagt ccacctgttt gattggctaa aatcggtttt
     7321 cttgtcttaa ggtcaatccg cataccaaga agagtattga tcatcgtaaa cccatctgcc
     7381 cctccagctt cgattgcctg agcgattgga acgatgtctg ttacatttgg ggacaatttg
     7441 acatagacag gaacagaaga aactttcttt actgcttgtg ttaattgaaa agcaacatca
     7501 gaatctgttc caaaagcaat tccgccatgt ttgacatttg ggcaagaaat gttcagctcg
     7561 attgctttca catttggtgc ttcgccaatt ttgctacaaa cttctacata gtcttcttca
     7621 caagcaccag caacatttgc gatgatgggt aactcattgt actcctctaa ttctggaaga
     7681 agtttatgca taacaacgtc tagaccagga ttttgcaaac caattgcatt caacatgcca
     7741 gatggtgttt ccgccactct tggcgtggga ttgccaaagc gggcatttgg agtcgttgcc
     7801 ttgaccatga tcgatccaag ctgattcaaa tcatagtatt ttccgtattc tttgccaaaa
     7861 ccgaaacagc cgcttgccgg catgatagga ttttttaatt ctaggccagg aagtttgacc
     7921 gctaaaggat tctcattcat ataattacct ctcctatttt aaaaatcggt ccttcgtcac
     7981 aaactttcac actttttgtt cctgtttcat cttccggcgt atgacaaacg caagcatagc
     8041 aagcccctat cccacatgcc atgcgggatt ctaatgataa ctggacattt tgatgcgaag
     8101 aaaacagctg ttccactgtc ttcaataacc cgtttgaacc acatgcaaaa acagctgtag
     8161 gttccgtctt cgttcctaac aataaattac cgacatttcc gtgcataccg tatgttccat
     8221 cgtcagtagc gattcttgtt tctcctagac gattaaattc tttttcataa taaacgactt
     8281 catgtgtggc aaaacctaaa aaatgcgttg gcttaattcc tttttcgact aactgttttg
     8341 ataactcata tagaggaggg acaccgattc ctccaccgat tataaatact tcgtctcctg
     8401 tatgaagatt ggacaaatca aaaccattgc ctaaaggacc catgacatct aaatggtctc
     8461 cggcagaaag agtagagaag actctagtcc cctcaccttc cacccgataa atgatacggc
     8521 aagtatttgt ttcgtggtcg atatgattca agctaatcgg tcggcgtaat agcaagtctg
     8581 cacgcggcac gagaatatgc aagaactgtc ctggttgctg catttctttt actagatcac
     8641 ctttcaatgt catttcaaaa atctttggtg ccagctgttt ttgctgaacg atttccatca
     8701 tttcttgttt catctgtccc ctcctgcaac ggtttttcgt ctttttgtct taacatgtgc
     8761 tcgccgttaa atagggttag ttgaaaaagc acgggattcc atcactcgaa cgatcgcatc
     8821 tgctgtgtct aaagaagtaa acaatggaat accatgttca actgcttctc ttcggatgat
     8881 gaaaccgtct ttagaagcat ttccccgatc tttatcaatc gtattgacaa ccacttgtgt
     8941 tttaccatct cttattaagt cgactacatt tttttctgct gcttcctcag aaattttcgc
     9001 tactgtttca acgaccagac catttttttc gaaataagca gctgtatgtt tcgttcctat
     9061 cagactatag ccaatttctg taaaacgttt agctaattct agtgcttctt cttttgtttc
     9121 gtccgcaatc gtaaataata cggctccata ttcaggcaag tgtaatccag aagcttcgaa
     9181 tgccttgtac aaagctttgt ctaattgtct gtcagatccc attacttctc cagtcgattt
     9241 catttcaggc cctaagtaag tatcaacttt ctgcaatttc gtaaaagaaa agacaggtgc
     9301 tttaacatga acttcttttg tttctggata aagtccgttt tcataaccaa gtgtctttag
     9361 tttctctccc aaaatagctt tcgtcgcaat ttgtgccatt ggaattcctg tcaccttact
     9421 taagaatgga acagtccgac ttgctcgagg attgacttcg atcacataaa cctcattttc
     9481 atggatgaca aactggatat tcatcatccc gacacaattc aaacctaatg ctaagcggat
     9541 cgtgtaatct tcgatcgttt tcttgatcga ttcagataat gtttgaggtg gatacacagc
     9601 cattgaatcc cctgaatgga caccagcacg ctcaatatgc tccatgatcc ctgggatgag
     9661 tactgtttca ccgtcacaaa tcgcatccac ttcgcattca ccaccaagca agtaacggtc
     9721 cactagtact ggatgttctg gagatgcttt cacagcatgt tccatgtaat cttctaaatc
     9781 tcgctggttt tcaacgattt ccatcgcacg tccgcctaat acatagcttg gacgaacaag
     9841 aactggataa ccaattctcg aagcgataac cacggcttct tctttacttg tagcagtatc
     9901 ccctaaaggt tgaggaatat ccaactcttt cagcgcttgc tcgaataaat cacgattttc
     9961 cgcacgatcc aagtcttcga tcgttgtccc taagattttt acaccagcag cagtcaaagg
    10021 ttcggctaag ttgattgctg tctgaccacc aaattgaacg attactccta tcggctgttc
    10081 tagctcgatc acattcatca catcttcgaa ggtcaatggc tcaaaatata atttatccga
    10141 aatagaaaag tcagtcgaaa ctgtttcagg attgctgttc atgatgattg cttcgtatcc
    10201 tgcctgctgg attgctttga cagaatgcac agtcgcatag tcaaattcaa ccccttgtcc
    10261 gatacggata ggaccagatc ccagtactag aattgagggc ttttctgatc gtgaactttc
    10321 attttcatat tcataggaac tataaaaata aggtgttttt gattcgaatt ccgctgcaca
    10381 agtgtcaacc atcttatata caggaatcaa atcattttct tttctcatct ggcgaacggc
    10441 ttcttcgttt atttcccaca gctccgccac ttttttgtct gtaaaaccgt tttgtttcgc
    10501 tgttttcaat atcttgtcat ccaacggctg ctgtttcaac tgctgttcga tttctacgat
    10561 atgcaacagt ttatctaaaa agaataaatc gatttttgtc aaatccgcta attcttctat
    10621 ggtgtaccct cttctaatag cttcagctac ataaaacagg cgatcatctt gtgctcgaac
    10681 cactttttgg attaggtcat cgtccgtgat gtctgccaac tcaggaagtt cgatatggtg
    10741 tgtaccgatt tctaaagaac gcacagcttt caataaagac tcttcgatat tccgaccgat
    10801 agccatcact tcaccagttg ctttcatctg cgtgcctaaa agccgttctc cgttttcaaa
    10861 cttatcaaac ggccagcgag gaattttagc tacaacataa tctaatgcag gttcaaattc
    10921 tgcaaaagtc gttcctgtaa caggattttt catttcgtct aatgttaatc caagcgctat
    10981 tttagcagct agcttagcga tcggatagcc agtcgcctta cttgctaacg cagatgaacg
    11041 agaaacacgc ggattgactt caatcacata ataattaaaa ctatgtggat caagagcgag
    11101 ttggacatta cagcctccct cgatcttaag tgctcgaata atcgaaagag aagcatcacg
    11161 aagcatttga tattcatggt cagacaatgt ttgactaggg gcaaatacga tagaatcacc
    11221 tgtatggata cccaccgggt caaaattttc catattgcag acaacgattg cattatcagc
    11281 cgaatctctc atcacttcat attcaatttc tttaaaacct gcaatacttc gttcaatcaa
    11341 acattgggta gcgggggaaa gacttaatcc attttctgat acgatacgta attcttcttc
    11401 attttcacac atacctccac cagttccgcc taaagtaaag gctggacgaa cgatgacagg
    11461 atagccgatt ttatttgcaa aatctacagc ttcttctact gtattgacga tttcagactc
    11521 ggggattggc tgattcaatt ctttcatcaa ctgtttgaat agatcacgat cttctgcttg
    11581 atcgatcgca gaaagctttg ttcctagaag ttctatgttc aattcttcta aaatacctga
    11641 tgctgccagc tccattgcca tattgaggcc tgtctgtccg cctaaagttg gaaggagagc
    11701 atctggacgc tcttttcgaa gaatccttga tacaaattca agtgtgatcg gttcgatata
    11761 tacttgatct gccacttctt tatccgtcat gatcgtcgca ggattagaat ttactaaaac
    11821 cacttcatat ccttcttctt ttaaggcaag gcaggcttgt gttcctgcgt agtcaaattc
    11881 tgcggcttga ccaatgatga tcggaccaga tccaatcacc ataattttct tgatatctgt
    11941 acgtttcggc atctgtttac tgctcctttc ctgcgtccat catttccata aattcatcaa
    12001 ataaatgtag tgcatcatgc ggaccagggg cagcatctgg atgaaactgc acactgaaag
    12061 cgggataatc tctgtgtttg actccttcta ctgttccgtc atttacttct acatgagtta
    12121 cgatgagttt ttctggatca atcgtggctt catcgactgc atagccatga ttttgagaag
    12181 taaaatcaat tcttccggtc gctacttctc gtaccgggtg atttaatcct cgatgtccga
    12241 atttcatttt ataggtatct gcaccgttgg ctaaagcaaa taactggtga ccgagacaaa
    12301 taccaaaaat cggtactttt ccttggattt ttttgatcat ttcgattgct tctggcacat
    12361 cttttggatc accaggtcca ttcgtcagca tgactccgtc tggtgatagc gctaaaatcg
    12421 tctcggcatc tgtgttatag ggaagtactg tgaggttaca gtttctttta ctcaattctc
    12481 gtaaaatact atgtttcaag ccgaagtcaa tgacaatgac tttccgacct gttcctggac
    12541 ttggataagg tttgttagtt gagacttgct gcacttgatt ggttgctaga acggttgctt
    12601 tcagctgatc aaatgcatgt tcgaaggaat ctccagcatc aactatacta gctttcatcg
    12661 ttccgacttc acggattttg cgagtaaggg cgcgcgtatc gatacctgat attccaggaa
    12721 ttcctttttg tttcaaaaat gaatctagcg tcatttggtt tcgccagttt gaagctacac
    12781 gtgcatgttc ttttactact actcctttgc atgttggaga aatcgattca taatcatctc
    12841 tatttacccc ataattaccc accataggat aggtaaatgt aatgatttga ccgttaaaac
    12901 tttgatcagt gatagtttct tgataaccag tcatacttgt tgtaaaaacg atttctccga
    12961 agacactagc atctgcacca aaagcctttc cttcgaaaac tgttccatct tctaatataa
    13021 gcaaacgttc catactttta ttctcccttc tgccacacaa gttttccgtt gacaaaagtc
    13081 atttgtgttt cgccttttac tttccagccg ataaaaggtg tattctcccc ttttgataaa
    13141 aagtcttctt tatcgatcgt gcaagtttgt gtaatatcaa aaactgctac atcagcgggg
    13201 gcaccaaccg ttagcgttcc tgcattcaaa ccaaaaatct cagccggttt aactgccaac
    13261 caattgatca cttgttccaa cgtgaatcga ccagtttcta caaaatgagt atagatcagt
    13321 tgaaacgctg tttcgcttcc tacgatacca aatggtgatt tcatgaaact ttggcttttt
    13381 tcttctaatc catggggggc atggtctgta gcgatgcaat cgatcgtacc gtcaagcaag
    13441 ccttcgatca aagcttctct gtcctcccgt cctcttagcg gcgggttcat tttccaaaag
    13501 ccgaaatctt cgggaatatc ttcgtcgatc aaaataagat gatggggtga aacttctgct
    13561 gttacgtgga ttcctgcttt ttttgcgtct ctgattacac ggacactttc tttagtagaa
    13621 acatggcaaa catggtaatg aacaccagtt gcttcagcta gcaacagatc tctagcaatt
    13681 tgagaagact cggttacgct taagatcccg ggaagaccaa gttcttctgc cttcttgcca
    13741 gcgtgcataa cgccgccaaa taataaagat tcatcttctg tatgggctac tagggcttta
    13801 ttgttttttg cagcttcttt catagccaga tacatcgtcc cagctgtttg gacacctaca
    13861 ccgtcatttg tgaaagcaaa agcaccttct tcaatcagtg cttcttgatc aactaatttc
    13921 tcacttcgca gattttctgt gattggcgca tactgcaata ccttcacaac tgcatctttt
    13981 ctaatgatgt catatacttg tctcagcttt tctgccgtat caggtacagg atttaaattc
    14041 ggcatcgcgc atacagtagt gaaaccgcca cgagcagctg agcgagtccc agcttcgatc
    14101 gtctccttgt atgtaaatcc tggctctctc aaatgaacat ggacgtccac taaaccaggt
    14161 gtgatcaatt gtccctttgc atcaaacact tcatcaaatt cggcttctga gaatcctgta
    14221 ccgattgctt tgattcgccc atcttctatc caaatttctg ctggtgtcgt catatttttt
    14281 cgtgtattga tctgaccgtt tttaatgagt gttttcattt tttcatcctc ctataattac
    14341 gcttttccat ttagcactgc ttctaagatt gccattctga cgaatactcc attgctcatt
    14401 tgctgaacga tccgcgactg taatccttcc actaatgaat cagctaattc tacatcacgg
    14461 ttaactggtg ctgggtgcat aatgattgca tgtttttgca acttcttcgc acgctcaact
    14521 gttaacccat gttcctgatg gtagccttct tttgagaaac tttcttttcc atcatggcgt
    14581 tcgtgttgca cccgaagcat catcatgaca tcaacttgat ctagtagttc atccaatggc
    14641 atataatgtc cgtacacttc gaattcatcg tcgtaccatt ctctaggacc tgagaagaat
    14701 acttgtgcgc ctagacgttt taacatctgc atattggatt tagctacgcg agagtgagtg
    14761 atatcaccta cgatggcaac tttcagacct tcaaaatgac caaattcttc ataaatggtc
    14821 atcaaatcaa gcaagcattg ggttggatgc tgtccgcttc cgtctcctcc attgataatc
    14881 gaacactgga ttgttttact ttgtatcagt tcgtcataat agttctcatc accatgacgg
    14941 ataaccgcta cgtctacgcc taatgctgac atcgtcagta ctgtatcgta tagtgtttcc
    15001 cctttttgga cagagcttgt gcttgcttca aattcaatca cttctagccc tagttttttc
    15061 tccgctacat cgaagctttt atgtgtgcga gtactatttt cgaaaaagag gtttgtagca
    15121 aagtactggc gcttttgcgg agtccaatta gcaccttgct tgaattcctg ccctctgcgg
    15181 atcaagccca taacttcttg atcagtaagt gcctcaacgg tcaataaatg ctttaagctg
    15241 atacgttctg atttgatgat cataatgttt cctccttagt tttcttcgct tcgtgaagtt
    15301 tctggtaaga taagatttaa aatgatgcca agtacagttg ctaatgccat tgccgacaat
    15361 gtaaatgtgc ctacttcaaa gacgagtccg ccaattccta ctactaagat aactgaagca
    15421 atcaacagat ttttcttttt atcaaaatcg attttgttct cgattaaaat cttcattcca
    15481 cttgcggcaa ttactccaaa taagataaaa ctgattcctg agatgactgg tccagggata
    15541 cttaaaatca aagcgcttaa ttttcctacg aatcctaaaa tgactgcaaa tactgcagct
    15601 ccaccgatca caaacacgct atggacacgt gtgatagcca gaacaccaat gttttcaccg
    15661 taacttgtta caggaggacc tccgacaaag gctgccacga tttgtgctaa tccatcacct
    15721 gtcaacgtct tatgaagacc cggttcttca aagaaattac gtttagttag tttattcaat
    15781 accataagat ggccgatatg ctctgtcatc gtcacaaaag cgatgggtgc cattgttgtg
    15841 attgctccaa gatatagctt tggttcatat tgtacaaagg gaatctcaaa gttaggaaca
    15901 gcaaaccagg cagcgtctac gattggctta gtatccacga ttccaaacag caaggctacg
    15961 atgtatccgc taataatacc tagtaaaatc ggaatcaatc cgagaaaacc tcttagccac
    16021 atgttgaaga aaattgttaa tcctaatgtg atcagcgcta cagcaatata cttgaaatca
    16081 tacgtgccct gattatacat agcatttgtt gctgcatttg ccgcaagtcc caacccgata
    16141 accatgatga ctggaccgac gacgattgga ggcaagattt tatccagcca tgctgaaccg
    16201 atctttttga tgatcagcgc aacaatcaaa tagactaatc cagttgtgac tgctccttgt
    16261 gcaatcgctg gataaccatc tgttttcatc aaaagctgca tcgctgcaat gaaagcgaag
    16321 ctgcttccta agtacgcagg tatctttcct ttagtagtaa ataagtacac caatgtacct
    16381 agtcctgaac tgactagcgc aatacctgga tcgataccta ctaagatggg gactaacacg
    16441 gttgctccga acatcgtgaa caagtgttgt aaacttaatc ctacccaatg tcctggggtt
    16501 gggcgatcgt ggatatctaa caccacgtca tcattatgaa atcctttttt ctccgacatg
    16561 tttctctctc ctctaatctt cctctttcaa aatcatgatg cgatcttgtc catcaagctc
    16621 ttgcatctct actaaaatct cttccgcccg agatgttgga atgttcttcc caacataatc
    16681 tgcacggatt ggcaattctc gatgcccacg atcgaccagg actgctaatg aaatttttct
    16741 tggtctgcca aaatccatta ctgcgtccat tgctgctcga atcgttcgtc ctgtatacaa
    16801 aacatcatca atcaaaataa cttcttttcc ttctaaggaa actgggatgt cagaagaatg
    16861 aagttccggt tcttctggat tttctttttt gtcatctcgg tataaagtga tatccagttc
    16921 tcctacagga atatcgatat cttctagctg cttcaggcgt tcggcaattc ttgatgcaat
    16981 gtagattcct cgtgttttga tacctaccaa tacgatatct tggatactat ggtttctctc
    17041 gataatctca taagtaattc gagtcaatgc tcgcttcatg gttacctgat ctactacttc
    17101 ttttgcttgc attttcattc cccctacttt tttgcataaa aaaactcctg ccctaagagg
    17161 acaggaggaa tgtttagact gagcgtatac tgatacactg atagctatac tatgtcaatg
    17221 agcaatctac ttatggcgca ccatacgtat ccttgctccc acccttctta gcctcacggg
    17281 actaaactta aaggaattct attaagtttt atgctttcgt tgttaattac tataccgtta
    17341 tctaaattaa aatgcaatgt tttttcttaa tttttctaaa gttttttcaa aaacttctgg
    17401 aagaggtgct tcaaagatca tcttttcgtt tgtagtcggg tgcgtaaaac ctaacatttg
    17461 cgcatggaga aactgaccat tgcctttcaa agttttcttc ggtccgtata ctggatctcc
    17521 agctaacgga taaccgatat acttcatatg cacacggatc tggtgtgttc ttccggtttc
    17581 tagttttaat tcaaccagag taaatccttc gaaacgttcc aatacagtaa aatgtgtgac
    17641 agccgacttc ccatcttctc ttatcgcctg cattttccgg ttttccttcg aacggccgat
    17701 tggcgcattg atggttcctt tttcgtgagg aatctctcca tggactaaag caacatattt
    17761 gcgcaaagag gtcttatctt ttaattgctg agctaatgat tcgtgtgcct ggtcattttt
    17821 ggcaaccatt agcagtccgg aagtatcttt gtcgattcgg tgaacaatac ctggacgaat
    17881 aacgccgttg atcgtagaaa gatccttgat atggtacatc agcgcattga ccattgtgcc
    17941 atgcgtatga cctgctgaag gatgaacgac cattccttga ggtttgttta tcaccgctac
    18001 atcttcatct tcgtaaacga tcgtcaacgg gatattttct gggaccacat ctaatgtttc
    18061 tggttcgggg atcaagacag taatctcgtc tccttgtttt actttgtaat ttgctttgac
    18121 tggctgtcca ttgacagaaa ccgctccatt tttcagccac agctgaattt gcgaacgact
    18181 gtaatcggtg aataaattag tcaatacttt atcgatccta ccagattcat ggtgaatcgt
    18241 cgttttaagt tctgtcattt ttttctttga tccttttcat ctaaaataat ataaattaac
    18301 atacaaatca ctccacatac aagtgtcaca tctgctacgt taaaaatcgg aaagctcata
    18361 aaatctgtct ggaacatatc tactacatag ccaagacgaa tacggtcgat aaaattgcca
    18421 atcgcaccag ctagaatcaa actcaatcca atcatgaacc agcgattacc tttttcacga
    18481 tttttaatca tcaatgtgag aatcacaacg actgcaatga gtgtcactat agtaaagaac
    18541 cacatcttcc cttctagcat actccatgcg gctccagtgt ttcgaatatg attcagtgac
    18601 aaaatacctg gtataagatt tttcgtctct ccaagcgcta gattatcagt gatccaccat
    18661 ttgaccaact gatccactcc tactagcaaa gcgctgaata tccaataaat aattaacaaa
    18721 aaatcaatcc ttttctattt gtttataaat atcacgaggt cgtacgatcc cgagcagatt
    18781 accatcggga ttcccatctt tagtgataag tacggcttcc aaagtccctt gctcacgaaa
    18841 catcgcttca acctcgtaaa tatgtgtttt agctggaaca aaatgataat ttgtgtactt
    18901 tccgttttgg atgatcaaat ccaacgctcg tttatttgtt aaatcaaccg gacctttttg
    18961 gctttctttt gctagccaaa acccgatatt acgtaaagta atcaatgcta caaattttcc
    19021 tttattgtat aacggaaatt gtgaatacct tttttctgct actatcttta acagttcaag
    19081 taacggaaga tcttgttcaa aacctgttac acgttttgca aatcgaggca gcacggtttc
    19141 aggctgaatc agttcttgtt cgatcagctg gattcgcttg actgtccaat catttggctc
    19201 tgcaatgaca aaatcgactg cgatccgatc atggacgatc gcattcctta actgagcaag
    19261 ctgtaaaaga tcatcttcgt attttctgat cattaattgt tttctttgag ctaggcgtct
    19321 gacaagttcg gtaaatccca tatttcgtgc atttcccatc tcgtctctca tccatttttc
    19381 gattcgattg aaactgatta aaaaaagatc agagtttgcc ataattttcc tccttgatca
    19441 aatatgcccg gttttttata tagaaaaaga acgcaatcaa cacaataact gcaaaaaaac
    19501 tgaaaaataa attttgtagg aaaacggccc ccaacccagt tgctagaaat aaaaacggat
    19561 agatttgaag aatcaagtaa ttcattgtga tcacataagc aggctttgct tctttcactg
    19621 tataaacggg gctttttagc caataaaagg tgacctgcat cgtaaagggc acttccagat
    19681 caagcgttat cttttgttca tgactgagaa cactgcgttt ttcattgttg attcttagct
    19741 gaatcgagct acccataccg taaaaaccgg tttttcttat aaatgtcgtt ttcatcatac
    19801 cctcctatta tgctttagta tagctcaaaa tacaataaag aaaaagaatg atcctagtgt
    19861 aaacaaaatt aattccctat cgtctaaaaa aagaagctgt agtcaaaata cagctccttc
    19921 gatggtagat tataatcaag cttaaaacaa tcccaacact tctccgtcat ttgtcacgtc
    19981 catattcaat gcagcaggtt ttttaggcaa accaggcatc gtcataacat ctccagtcaa
    20041 ggctactaaa aatcctgccc ctaattttgg gatgatttcg cggatcgtga tggtgaaatc
    20101 ttccggtgca cctagcagtg aaggatcatc agaaaatgaa tactgtgttt ttgccataca
    20161 aatcggcagt ttgtcccatc cattcttcgt aaactcttct atttgttttt gcgctttttt
    20221 gctgaaaaca acaccatttc ctccgtaaat tttcttgacg attgtttctg tcttttcttg
    20281 tatcgtttct tctaggcggt ataatggctt gtaattagct tcttttctat caatcaatcg
    20341 tactacagct tttgctaaat caacgccgcc ttctgctcca tctgcccata cactcgctct
    20401 cttggcaaga atcccctgat tttcacacaa gtgctccaac agtgtgagtt cattgtctgt
    20461 atctgttacg aattcattga ttgcgacgat cacaggtagc tgatattgtt ccatattacg
    20521 tatatggcgc tttaaatttg caaaacctac tttcaaagca tcaagattct catttttaag
    20581 ttcatctttt ttcattccgc catgcatttt caatgcacgg acagtggcga caatcaccac
    20641 agcatccggc gcttttttca agttaggtac tttgatatct aaaaatttct ctccgccaag
    20701 atctgcacca aatcctgcct ctgtgacaac gtaatctgct aatctcaatg ctgtcttggt
    20761 agctaaaata ctattgcagc catgagcgat attggcaaac ggtcccccgt ggacaaaagc
    20821 tggtgttccg taaattgttt gtacaagatt cggcttgatt gcatctttca gcagtaatgc
    20881 cagagcgcct tctgctttca agtctccagc agtcacaggc tgacggtcaa atgtatagcc
    20941 gacaacgatc cttgcaagac gagcctttaa atcttcaagg tctgccgcaa gacacaagat
    21001 agccatgatt tcgctggcaa ctgtgatatc aaacccatct tctcttggaa caccttggat
    21061 tggcccaccc aagccaacaa ttactttacg cagttcacga tcattgagat cgactacccg
    21121 tttccaaatc actcggcggg catcgatgtt caattcattt ccttgatgaa tatgattatc
    21181 taaaagagca gataatgcat tattagctgt tgtgatagca tgcatgtcac cagtaaaatg
    21241 caagttgata tcttccatag gcaatacctg tgcatatcct ccgcctgcag caccaccttt
    21301 gattcccatc acaggaccaa gtgagggttc tcgcaatgca ataacagatt ttttattgat
    21361 tttattgagc gcgtctccta accctacagt gatcgttgac tttccttcgc cagctggtgt
    21421 tggattgatg gaagtaacta agatcaaatg cccctcagcg ttatcctttg atcgattgat
    21481 tccagaaaaa ttgattttcg ccttatattt cccatacagt tccaaatcgt cttccttaag
    21541 atctaattta ctagccactt caacaatcgg ttctagctct gtttcttgtg aaatttgcag
    21601 atcattcttc atttgattac cccttttcac gaactttatt caacaaaata tcttctttgt
    21661 taagatatgc caattatacc tttttttatt cagaaaaaaa acataaaatt acaggttttt
    21721 aat
//
LOCUS       DACCWK010000038        21384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCWK010000038 DACCWK010000000
VERSION     DACCWK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.038"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(47..349)
                     /locus_tag="IVW65_001383"
     CDS             complement(47..349)
                     /locus_tag="IVW65_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7900267.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(375..806)
                     /gene="ruvX"
                     /locus_tag="IVW65_001384"
     CDS             complement(375..806)
                     /gene="ruvX"
                     /locus_tag="IVW65_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7900268.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(803..1069)
                     /locus_tag="IVW65_001385"
     CDS             complement(803..1069)
                     /locus_tag="IVW65_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7900269.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1323..2048)
                     /locus_tag="IVW65_001386"
     CDS             complement(1323..2048)
                     /locus_tag="IVW65_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7900270.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2414..2484)
                     /locus_tag="IVW65_001387"
     tRNA            complement(2414..2484)
                     /locus_tag="IVW65_001387"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2450..2452),aa:Gly,seq:ccc)
     gene            2601..3353
                     /locus_tag="IVW65_001388"
     CDS             2601..3353
                     /locus_tag="IVW65_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7900271.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3369..3848
                     /locus_tag="IVW65_001389"
     CDS             3369..3848
                     /locus_tag="IVW65_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7900272.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4099..5679)
                     /locus_tag="IVW65_001390"
     CDS             complement(4099..5679)
                     /locus_tag="IVW65_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7900273.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5672..6415)
                     /locus_tag="IVW65_001391"
     CDS             complement(5672..6415)
                     /locus_tag="IVW65_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900274.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6591..7367)
                     /locus_tag="IVW65_001392"
     CDS             complement(6591..7367)
                     /locus_tag="IVW65_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900275.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7615..8451)
                     /locus_tag="IVW65_001393"
     CDS             complement(7615..8451)
                     /locus_tag="IVW65_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7900276.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8462..9391)
                     /locus_tag="IVW65_001394"
     CDS             complement(8462..9391)
                     /locus_tag="IVW65_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7900277.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9583..9936
                     /locus_tag="IVW65_001395"
     CDS             9583..9936
                     /locus_tag="IVW65_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7900278.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10010..10666)
                     /locus_tag="IVW65_001396"
     CDS             complement(10010..10666)
                     /locus_tag="IVW65_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7900279.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10892..12826
                     /locus_tag="IVW65_001397"
     CDS             10892..12826
                     /locus_tag="IVW65_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7900280.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12912..14135)
                     /locus_tag="IVW65_001398"
     CDS             complement(12912..14135)
                     /locus_tag="IVW65_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7900281.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14129..16027)
                     /locus_tag="IVW65_001399"
     CDS             complement(14129..16027)
                     /locus_tag="IVW65_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7900282.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16021..17139)
                     /locus_tag="IVW65_001400"
     CDS             complement(16021..17139)
                     /locus_tag="IVW65_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7900283.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17222..18055)
                     /locus_tag="IVW65_001401"
     CDS             complement(17222..18055)
                     /locus_tag="IVW65_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7900284.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18048..18965)
                     /locus_tag="IVW65_001402"
     CDS             complement(18048..18965)
                     /locus_tag="IVW65_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7900285.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19192..20316)
                     /locus_tag="IVW65_001403"
     CDS             complement(19192..20316)
                     /locus_tag="IVW65_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7900286.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20499..>21384
                     /locus_tag="IVW65_001404"
     CDS             20499..>21384
                     /locus_tag="IVW65_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7900287.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPS"
BASE COUNT         6648 a         4241 c         3511 g         6984 t
ORIGIN      
        1 ttttcagaga gtccagcttt tcttacacct tatcgattct tttgttctat tcttcttccg
       61 ccatgaatgt attgaatact tcttcgatca tatcccattc tgcgtctgtt tcgatttgtt
      121 ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt aattctacat
      181 cttcatcttc tgaaacacca gctggatata aaagaacata gttacgtcca aattcttctt
      241 gtccgtcaac tgttagcaag atttcgtata atgtttcatt tccttggtca tccaccaatg
      301 tgatgtgttc gtgtccttcg tgttcgtggg aatggttatg ttctgtcata agattacctc
      361 ttttcttttt attattaaaa taattgctta cctgagccgt ctaaataatt ttgtaaaatc
      421 atcacagctg ctaatttatc gatgactttt tttcgtttcg ctcttgaagt atttgcctgc
      481 tctactagca tacgttctgc ctgaacagtg gttagtcgtt catcttgata atcaaccggc
      541 aaggagaata gttctttgat cttctctcca tatgacatgg aagcttctgc ccttggtccg
      601 atggaattgt tcatattctt aggtaatcca actacaaaac ggtctacttc gtactgcgcc
      661 actaattcct tcaaccgatc aaaaccaaac tcttcttctg cctcattgat tcgaatgatt
      721 tctactcctt gagctgtcca gccaaaggga tcgctcacag caacgcctac tgtcttagaa
      781 ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg tagtctttga
      841 caattacttc catgatttca tcgcgttcgt gacggcgaat caaatttcgt gcatcacgat
      901 aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga ttgattggat
      961 tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc acttcttttt
     1021 tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg attctacacc
     1081 tctttcccgc aaggtaattc cagcttacct tctacaccct tattttactc gaagaaaggg
     1141 aaaactacaa acaaatattc caaacttctg aataaattaa gaaacacgat ttgtctgatt
     1201 tttccttttt tctgtggctt tttcaaaagc cctctttcca taaagtatac gctatatttc
     1261 tttgaaagta tgtttttaca caattttttc ataaagttca taaaaaattc gaattgctgc
     1321 tactaatttt tcgttttcgt tgcttgccct gtctcataat tgatggtgta gccatcttga
     1381 acattgtaga taaagacatt gaattcaatt tttttgtctt caatgctttc tgcttccaac
     1441 tgcacacctc ttgctaccag ttcatttccc tcgaaatgag gagtgacacg atagcgtaca
     1501 tgatgatttg tttccttgat atatgccgca atatcatttt cgtgggcaag catgtacgga
     1561 gcattcaacg aacgtgtccc tgtcattaag tttttaatgt tattattttg accagtcaac
     1621 tggaaaccaa tcaaatggct gcggttgtat aaccattgtc cgtcacttaa tttcttttgt
     1681 ttccaacctg tcggatcaat atacaaaggt tctctctttt cagtaggaaa caagtctttt
     1741 cccaacatcg cattggcttg tcctactctg ttcaatttgt ctatatttga atagctttcc
     1801 catgtccctt tggaaagact aagatcctct tttgtaaact ccggctgatt cccattgatt
     1861 tctatttctt gatgttttcc atcatattct ggcacatccg ctacttgaga agcttgcggt
     1921 gtggaatcaa aaatggttgt aatttgttgg aagatatctg aatctttttg tgtggtggaa
     1981 ataccgagtg cccctgcaat cacagcagcg acgatcccca cgatcgcctt ggttgttttt
     2041 ttgctcattt ctttactcct ttactaacag ttccctaaat cttaacatat gttcccctat
     2101 aaataaatac tggaaagaaa tagaaacagt aaaaaaataa aaccagtttt tagacggctt
     2161 aagaatacaa aaaaagagac ggattcatcg tctctactca atcacaaaac actatttaca
     2221 aggtatttat tatcattcaa aaaatttttc ttaatactca aaactaaacg gcttctgtac
     2281 gacctcttgg taaacagtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtca
     2341 aaaacataag aagctttttt gcttgactat tcttattact tgatgcaggg cagctttttc
     2401 acagcctctt ttaagcgggt gacgggaatc gaacccgcga catcagcttg ggaagctggg
     2461 attttaccac taaactacac ccgcgataca aaaacagtat acgcctcttt cactccttcc
     2521 tcaagttttt ttattcttta cgcttccgat atgttttgta gtaaaataaa actaatcaat
     2581 caattattgg agaagatgct atgacgggaa aagaattaga tctgttgctg gacacttgtt
     2641 tactcgctgg gaaaatcatg atggaaagca atgcggagat gtatcgggta gaagatacga
     2701 tgagtcgaat tgctttagct tctggaaatt tccgtttggt cagctatgtc actcaaaccg
     2761 gtctctttat tggtcttgat cgtacctcga ccatccgaat ggaacaaatt accaaccgtt
     2821 cgattaattt agaaaaagta gtaaaagtaa atgatctttc acgtaaatat gttgcttcag
     2881 agctaacatt agaagaactt tactgcgaac tgcaagcaat cgaccgagac cgttctttct
     2941 ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag tggaacaatt atggtgttat
     3001 ttggcggaaa gatgaccgat ttacctgtga ctttagtcat tggaggattg ggatacgcag
     3061 cttatctata tagtttgaaa ttttttagaa tcaaattttt atcggaattt ttatcttctt
     3121 tattgattgg catagccgct atctacagcg tacgttttgg cttaggaagc aatcaggatc
     3181 taatcattat cggatgtgtg atgcctttag ttcctggagt tcaaattacg aacgctatcc
     3241 gtgatttgct agctggacat tatgtctcag gagtttctcg cggaacagaa gcgatgatga
     3301 ctgctacgat gatcggtttt gcgattgctt ttattttcca gcttttttat taggaaagga
     3361 acacaagtat ggggacgata tttattcagt tttcttttag ttttttagcg actgcggcat
     3421 ttgccgttat tacaaatgta cctaggcgtt cattgatttg ttgtggtctt tctggtgctt
     3481 ttggctggat gatctactgg atctgtgttc aaataggcgg atctgctgct tttggttcat
     3541 tgcttggttc tttaggagta gcattcatca gcgatctttt ctctaaacgt ttgaaaatgc
     3601 ccgtaacgat tttcaatatt cccgggatgg ttcctctagt ccctggtggt ttagcttatc
     3661 aagcggttcg aaatctcgtt actgaatcct atcgagaagc ggctcattat gcggtccaag
     3721 caattatgat tgctggagca atcgctttag gtcttgtatt atcagaagtc tttaaccata
     3781 acatccgtaa tttcaaagta aagcgtgacg caatcatgat gaaacgaaaa aaacggcttt
     3841 ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga gacgttcagc cttgagcgat
     3901 gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg agcaataaga agtaattatg
     3961 agtattaaaa aaagttttgt aaaaatagct tctcacattt ttacataaaa ttgacttaat
     4021 actgagaaac tgacttttga acaccctcta tctgtaaaaa aggggctgcg acatgatttt
     4081 tgtcacggcc cctcttcttt attcaaattt tttccaaaac gttcgttgat aataaacgat
     4141 ccatagagcg gaaagtatta gaactaaacc gatcacagga atattcacta accaaaatgg
     4201 ataaaacaaa gccaacattc cgtataagac gataagaata atactgatga tcaatgaacc
     4261 catcatagtt gcgactaaac ccaaacttcc tgaacctctg ttaaataatt ggttaatatt
     4321 ggtccagttc agcagcagca accgataatc tctagcgaaa taacgtagtg acaataagta
     4381 gcagccgaat aaagaaccaa atagcaagct agggataaaa aacaaaggca gctgaaaaga
     4441 cagtccgctt aataatgcga tcccaccagt taaaaaactc tgcaatatcc atccgaatct
     4501 gaacttttct tttaaatatt ggttcataga gattggcaaa gaacggataa acagaaaatt
     4561 ttcttgatcc aaagaaataa gattgctgat aaaagaagtc tgattaactg ttaagaaagc
     4621 caatgcgatc cctaccagga aaacaacacc gattaactta ttatctaaat cagttaattt
     4681 tatttcgcct cctagggcaa aagcaagaat aaaaatgatt ggagttaaca aggaagaaga
     4741 aagaacttgc ataatcaaat taggttcttt tactaattga gcattgtaac taaataaaag
     4801 ctgatgcaca ttttggttct ttttatgggt tctttttatt ttgaactcac tgtttgaatc
     4861 agtcagttgc tctgtaagct ttggtactat cagctgtttt acagcaagga aaaatcccag
     4921 agccgtgata gctagtattc cccaacccaa taaaccttgt tgagaaagtg gctgactggc
     4981 agcatcatag aatggcaata atatcgaaat cggatgccga tctgttaact cattttcaat
     5041 agtccccgtt tgatgattca tccacatgat tccagcaaat gcgatcagca tcgagctgcc
     5101 aagcaagata ctagaaactg tttttctatg ttttttaaac actgctgttc gagccaaacc
     5161 aaaaacaatc aaactagcca gacaaaaaag aataatcaaa aatagagaaa acaaaaggat
     5221 tgagataatg atagtaagca aaatccctag atcagctcgc catccggtta aaataaacat
     5281 gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca actaccaaaa tctttgctga
     5341 aaaaacctgt tcttgtccaa aaggcagagg caaaaatact tgcaggtcat ttccttcaaa
     5401 aaagatatta taaatgactg atatactttg agttaaacct aacataccaa aaagtgccac
     5461 gtaataagta aagaaacctg gcaatttaac aaagttcata gcaaacatag caaatccgta
     5521 aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac aaaagataga tagttaatcg
     5581 ctttcccttt tttcctttct ctcttgcttt attcgtgagc tgtggattaa catataaaag
     5641 attaactcga attaattcta ataattgtcg cttatacata cggtccatct cctgtcgctt
     5701 gccttccagc cattttcaaa taaatctttt ctagcgtttc atcaggtgag ccattcaata
     5761 attcactaac tgccccctga taaatcaatt cacctttctt caaaatagcc agtttatcgc
     5821 ataactgttg tgcggtatcc aatacatgtg tagaaaaaat gactgttttt ccttttgcgg
     5881 catgagcctt catcatttct ttcaaatcaa atgctgcttg aggatcaagt ccttgcaaag
     5941 gttcatccaa tacccaaata tctggatctg gcagcaacgt gccgatcaaa atcgtttttt
     6001 gacgcattcc atgtgaaaaa cttgcgattg tttcattttg atgactatac atatcaaaaa
     6061 gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt gtctaattca taggcagctg
     6121 aaatcaagtc ccaatattct ccagccgtca attgtaaaaa aatatctggg gaatcaggca
     6181 cataaccgat tttttgtttg atactcaatc gattttccgt caatttcatt ccatctacta
     6241 aaatcgttcc cgatgatggt tcaataatac taactaaact tttaatcgta gttgattttc
     6301 cggcaccatt atgtcctaaa aatccaaaaa tttccccttg tcttatcgag agagacaagt
     6361 cttttaatgc ttgttttttc ccgtaacttt tacttacatt gatgaattca atcatttctg
     6421 tactcctttt gtttgttatt actttttatt gtacccttcg ttattggtgg agtaaatgtc
     6481 aaaaagagtg cataacaaaa aaggatagaa acgactaggc tgctacttgg gttgttctct
     6541 tctacaggaa aacactcttc taacaggtaa gatatgagtc tttgttttct ttaaaatgat
     6601 tcattttcta tcttttcttg cataaaattc tttagtttat caatacttct ctttcccatc
     6661 tctagttgtt ctccatttgc aaatgttact atttcgttta ctctgctaat agatgtaatg
     6721 ttttcagtat taatgatata agcttttaaa tctttacata aatactcata ttcgagtgat
     6781 tttttatatt ttttaattgt gccgtttacg agaatctctt catctttcaa tactaggcct
     6841 attttatttt tataacctgg catgctcatt agataaagga tttcatcgta atggatatag
     6901 tgatcgctat taccataacg gacagttgtc cacctttttt tcgtagcatg ttgaacagga
     6961 tagttttctt tttctttcaa taagtaactt tggatgcttt ttttattttt ttcataagtg
     7021 aattctttta tcaaataacc actaggcgta atctttcgat taataacagt tattgcttta
     7081 ttcggatgag acgttagata aacaatttta cacttttcat tcttttctct aagcatttcg
     7141 cctagttcga taccgctata ataggcgttc aaatcgatgt ctaggaaaaa aatatctgag
     7201 gaatagattt cattattttt aatgaattca acaaattgta taacgttcaa tccctgaacg
     7261 atctcaaatt tccaaggact taataaaatt ctggattctt ccaaaatttc tactagcatt
     7321 ttttcaaaaa ccaaactatc atccaaaata aatatacgcg ttaccataaa aagtacctcc
     7381 actcgatatt ttactatcaa aatcatttca tacaactttt caacatacat gttaggaata
     7441 aattgtaatt ctcttcacaa tcttttccaa cacctttgaa accgatatca aatactttca
     7501 ggatgctgct tctaaaataa atatcaaaca aatgaataca tcatgcaaaa aaagccagac
     7561 aaccgacgtc cttgcgtgtc agctgtctga gctatgttta tttattttgt tcttttaaaa
     7621 agaacgatac acatactgat ttttcggagc agagactttc tcagcgtttg taacaactac
     7681 agatgggatt tcacgcttga atggttgata atagtctgat tggatggttc cttctatttt
     7741 ataccattca tcatttttaa ccgttactcc ttccggcata tgcgtcaaca aaccaaagac
     7801 tcctgaatct gccacacaat ggataatgcc gaatcggaac aagaagatat ctgtattgct
     7861 atcgttcggt gtttggtaaa caaatccttc atagctgatt tttttgccaa tgaattcgct
     7921 agggtaatta taaattagct ccataacttc taaatagttt tcatctgtga tcttgatagt
     7981 ttgcttatcc ccatactttt ctttcaactc attcatttgg tctaagtagt ctgatttgtt
     8041 gaaataaata ctggtatccg gctttaagta ttgtgtctgc atctccggat cccctactga
     8101 ttctttactt actgggaaat taaatccttt ggcttctacg atcgtagtgt cgagactaac
     8161 tgttggaaac aacgtcccca cgattactgg caaagctagc agtacataag cgattccacg
     8221 ttgataaggt tttgacaacc catggtcatg atcgtgctcg tgtgcctttt cttctgcttt
     8281 tccgtttttg acccacaaat acagctgaac taaagccaaa agagcagata aaatcattga
     8341 aagaatagct aagtagcgat aatggacatt gatatattga tcgagtttgc cagaaacttg
     8401 caagtacatc atcaattcga aatacccaga taaaatcaaa aaccgcagca tattttccct
     8461 cctaaataaa taatgtgaat ccaataacgc taagggcaat cagtccgatc agcgataaaa
     8521 taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt tttgatatcg accattgggc
     8581 caaagaccaa aaaggcagct acaggtgctg tgccgaacag acttaacaaa gaagatccta
     8641 taaacgcatc tgcttctgag caaagagaca tcgtaaaggc caataacagc atgatgataa
     8701 tggctaaaat ctttgttgag ccgagcgtta gcataattct agttggtaaa taagtctgca
     8761 tcgcagctgc gattaatccg ccaatgatca aataacgacc tgtatcaaaa aattcgtcga
     8821 ttgaatgtgt caacacatgc ccagtttgat ggatcagctg attttttggt tcattcttct
     8881 cactttgttc ttcttcatgg acatgatgat ggtgattttc tgctacttta gacgtgattt
     8941 tttctttcaa tacagcgtct ttttgaaaat aggcaagcca gacaccaatc acaagggcaa
     9001 caaaaaagct gcctatcacc cgccagcttg ccatctgcca agaattgcca aatgcgataa
     9061 aagtggaaaa aatcacgatt ggattgatga tcggagcagt cagcataaaa gcaaacgctg
     9121 tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat cggtacgatg ccgcattcac
     9181 atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat agagaagata cgattttcag
     9241 gaagccatct cttcacacgt tctggggtca aaaatacttg taaggcgcct gagataagac
     9301 agcctaataa tacgaaaggt aatgcttcaa taacgatcga taagaaaatc gtactcatct
     9361 gcaaaaccga atttggaata aaagaaaaca tgagtttcct cctttttatt tctagttcat
     9421 tcgctttgca aatatacgct tttattcaaa aaaaacaagt tgctctgtaa atttttacac
     9481 aactcttatt ttttaccaaa gcacttcaat tgactttttc tgttattccc attaaattaa
     9541 ggtctcatac atatcacttc tatcataaaa gggagaataa aaatgaaaca aaccactaac
     9601 ttgactgaag ttcaagacat actgcaacag tcatccgttg ccgtcattta tctttcgatg
     9661 cctaattgca gtgtttgtca cgctgttcgt ccacgactag aggaacttct cacacattat
     9721 gatattccgg cacttcattt ggatgcattt gaaacaccag aagtagctag taaatttgaa
     9781 gtgctgactg cgccagttgt cctgattttt catcaaggaa aagaagtctt tcggcaagca
     9841 cggttcatcg actttaaaaa aatccgttat ctactagatg aactgacagc agaagatgat
     9901 tccctttctt atgaagaaat cttccgaaca aaataatcgt tgtccaaaca gaaaaaagcg
     9961 attgaacaag tgaaagtttt gccttcattt gttcaatcgc tttttatttt tattctgctg
    10021 tattttcgga atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt tgcaattcat
    10081 tcgtcaaatc aacattttgg atcaatacat ctgacggagc attcaatcga gcagcggtaa
    10141 agttcaagat tcctttgatt cctgcttccg ttaactgatt gactacttct tgtgctcttt
    10201 cagcgggtat cgttaaaatt gctacttcga tttgttggat cttgatttgt tctgacatat
    10261 cttccattgg ataaacgggg ataccgtcta cgatgcgccc aacgatttcc tcattcacat
    10321 caaaagcaca actgatacgt atgctattac tttgatggaa tttgtatttt agtaaagcac
    10381 ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg tttaatgtct
    10441 ttgcaaaaaa gtgcataaga tcttctacat cataaccata cccacgtttt cctaattcgc
    10501 caaaataaga aaaatctcga cggatcgttg cactatctac ttgaacggct tcacttaatt
    10561 cagttgaaga cacttttttc tttccagcat tatttaaaat ccttaaatag cgataataca
    10621 aaggcagtct tttagctgtc gcctttggaa taatatgatc tttcacgaaa tgacctccac
    10681 gtattgtcta attacatctt tgatttctcc atcatagcag tatttatagg aaaaaatcaa
    10741 ttccattgct cagatattgt gaaattttat tctttattca tttatttatc atttctgcaa
    10801 atctcgcagt ataatggaat ttatggtaga ctactaattg aaaaatagtt ttttatttga
    10861 ggagaattca caaagtagaa aagaggattc catgatatta ttacaagcca atcagattgc
    10921 tcgccatttt ggtgcagaag tcttatttga aaacatacat ttagaaatca gtacaggcgc
    10981 tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg actttattaa agatcattgc
    11041 tggaattgaa gcacctgatg aaggaaagat cgctaaaaat aaaacagcga ctctcggata
    11101 cctcgctcag gacacaggac ttgattccaa tgaaacagtt tggaacgaga tgctgaaagc
    11161 ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag ctagagattt tcattagcga
    11221 aggtacgcca gacacacctg cttatagttc ttatttaaaa gaatacgacc ggctgcaaca
    11281 cgatttttca gatcgaaacg gctatggtta tgaaaatgaa attcgttccg tattgcatgg
    11341 gtttaaattc gattcttcat tttatgatca gaaaatcgat actttatcag gcggacagcg
    11401 cacacgttta gctttagctc gcatgctttt gcaacaccct gacattttga ttctagacga
    11461 accaacgaac catttagata tcgagacact ttcctggctt gagaattatc tgcaaagcta
    11521 tactggtgca ttattgattg tttcccatga ccgttatttt ttagataaag tagtcaatga
    11581 agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt cgaaatatct
    11641 agatttgaaa gcagaacagt tagcaagtga atggaaggct tatgaaaagc agcaaacaga
    11701 aatccataaa ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta ccacaaaacg
    11761 agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc ctcaagggga
    11821 tgaaaaacaa gcaaactttc tattcagtat agaaaagcct tctggaaatg tagtcttgca
    11881 aatcgaagat gccgctatcg gatatgataa tgtcacctta tctgaaccaa tcaatctaga
    11941 tatccgtcgt caagaagcga ttgctctagt aggtccgaat ggtatcggga aatcgacgtt
    12001 gctgaaatca atcattcacc agatcccttt tatccgtgga aaagaaacgc tagggacaaa
    12061 tgtcaccatc ggctattacg atcaaggtca agctgatctg catacaaaca agacgatcct
    12121 agcagaattg tgggatgagc atcctaccac tccagaaaaa gagatccgaa gtgctttagg
    12181 tgctttctta ttttctggcg aagatgtaga aaaaccaatc cctctgctaa gtggcggtga
    12241 aaaagcacgg gttgctttag ccaagttagc gatgaacaaa gaaaattttt tgattttgga
    12301 tgaaccaaca aaccatttag atattgacaa caaagaagta ttagaaaatg cattgatcga
    12361 ttatgacggc acactgcttt ttgtttccca tgaccgatat ttcatcaatc ggatcgcgac
    12421 aaaagtcgtc gaactctctg aaaatggcag caaagtctat ctaggagact atgattatta
    12481 tttagagaaa aagcaggaag aagaagaaat cgccgaattg ttggcacaag atgagccaga
    12541 atctgctcct gctcccacga acaaatctga ttattaccaa agcaaagaac agcaaaagct
    12601 tatccgtagc cttcaacgaa aaattactca ggttgaagaa gaaatggcgc ggatcgatga
    12661 atggatcgat cagttggaac aagaaatggt cgcgccagaa aacctggaag atcacgtaaa
    12721 actcaatgag ctgaaccaag atttagaagc tgctagacaa gatcaagaaa caaaattgac
    12781 ggaatgggaa gaactaagct tggaattaga agaatatgaa aattaacgaa aaaaccaaaa
    12841 cagaaaccat tcatgctgtt ttggtttttc tttttttatt ttatcaaaca aaaattccat
    12901 atttaccttt ttcaccaata agaagaccaa ttactagtca ttctcactaa agcttctaaa
    12961 taatagtaat caccccaaat acaaggtgta tttaccccat tatttttggg cttattataa
    13021 acagactgtg ttaaaaagcc atcttcttct tccgttgtag catatctttc tttcaattgt
    13081 cgcataattt tccaaacagc agactggtat aagttctttt ctgttttgtc taatagacct
    13141 gattctatta attctaataa tccacaaacc atgatcacca tactagatgt atctttttct
    13201 tctctgctag aatcatcaaa gatcaaatcc caataacaaa tggtatcttc tggcaaccga
    13261 ttcataaaat aattggctaa tattttgctt aaatcaaaat atttttgttc tttcaaataa
    13321 cgataactta atgcaaaacc gtaaatcccc catgcttggc ctctagccca acaggaatta
    13381 tcactataac cttgatgcgt acttccatat cttggttttc catctttgac atccatataa
    13441 aatgtatgat aagttgaacc atcttctctt actaaataat ctgctgcatt ttgaatatga
    13501 gcctgagcag cttttttata tttttgattt ccagtttctt cactcgccca atacaataat
    13561 ggaagattca tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc actaggatct
    13621 ccccaagctt gtataactcc tgccttctcc caaaaacgct gaagtaatgt ttctgcagca
    13681 tcaattgaag cttccttcat ttttcgattt cccgttattt tataatctgc tacggttgat
    13741 aaagaataca aaaaaccaag atcgtgatga ttagtgataa tttgacagtc aatcctgtgg
    13801 ataaaatcta tatctgcttt ttcagctgtt tgttgaaata aaggatcacc cgtcttctca
    13861 aataacaacc aattaattcc tgtccaaaat ccttctgtcc aaccaatatt gtctcttaat
    13921 ggataacgtc cttttatagg acactcgctt gggaattgat actgactgtt actgatacgt
    13981 ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct cattattcca ttctttttcc
    14041 attaattctt tataacattc atttatccat ttttctggat gtattatctc ttcaaacatc
    14101 actttatcaa gcatttcccc accagccttt agcataattt tagtcttttt ttattgtttt
    14161 tattttggat agttagcccg gttgtcccat caaaatcaat tttgggacga ttgttccata
    14221 cttctaaact ataatcccga tcttgatgag caaccactcc tgttccgagc caatgggttc
    14281 cttttccaaa tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc atcaagtttg
    14341 tattgggcaa acccataata gctgtggatt ttcgtttaat agtatcgtca cttaaacaga
    14401 taatacctga aaagccagct tcactcaccc tatagttttc cttgtcttct tctatgatta
    14461 ttccttcttt ctcaggagaa cattcaatag caaaccctgt ctcatataga acatattctg
    14521 tatctaattc tactttgtgt acgcgaatct gccagccttc aaaaggaata agccaagttc
    14581 tgatttttgc ctgtaaagta ggttcccatg tagaggcaag tacctctttc ttacatacaa
    14641 tgtctcgtac attatttcga acgatgaact gtccataggt tttgatgctc tcattcttaa
    14701 aactggtcgt gatatatgtg tccgctcgct gaatccctag cattgaatcc attgccattt
    14761 ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa taagcaaatt
    14821 ttaaatattt ttcattggtc aatgtatgga aatttggccc taattgtccc gcattcaaaa
    14881 aaaatgtatg tccattatca tgttgtatga gcaaattggc ttctgaaaga agtttagttc
    14941 cttcacgaat aggcataggc atttctttag ccgtccaata ctcatggtct tcaggtagcg
    15001 ccaacaataa aaaaatctta ttcagccata atggagataa cgtactatta tagggttcag
    15061 tcaatgtcaa ttgagggtag gtataaccaa tattcaggat acctttttca tcaaatattt
    15121 ctttttgcat ccaccatctt atattccgat tgactaaccc cttgatttca cctaatttaa
    15181 aaggcaaaag atcggcccac aacatagctg accaaaagct tgaaactgca aaacgataaa
    15241 tcatgcttcg accaaaaggc acatttgcac catcttccgc aaaaaaatga atatagtctc
    15301 tagaaaaatc aatagctcgg ttcttatata tctggctaat gggcttttct ggttctagta
    15361 cgctataaat caaaccataa taatgaaatg caaaaggatt ataataatct tggcgacctc
    15421 ttgaactgtc tcgataccag ccattttgaa gataacaatt ttctatatta tcaaatgctt
    15481 ctttcagtgc tgattcttta actggataac caaatttttg gataattgtt ccgacaatta
    15541 tcttaaaaaa ttgccaatta ccatctgcac attcatattc aaaaatttgc gagaaccaat
    15601 gaataatctg gcttttttct cctgtatcta attttttcca taataattgt tcatgatgat
    15661 ataacgctaa taacattgcg ggcatttcta ccatacgctg gtctctttca tgtatttctc
    15721 cccaataatc gggatgggaa ggatctgttc cagcttttat tcctgataga attaatttca
    15781 accatcgttc atctaattgg tcaattgcgg gccccgctcc ccataaaacc cgagagaatc
    15841 cttccattcc tgcaatttta tttccatatc ctaccccttg atgtatataa tcaatttgtg
    15901 cgccacttgc tcgataaaac ggctggagtg gttgatttat ctttttaaat aagtcaaagt
    15961 aatcctgtct ggtccttaat agtggtactg gatgtttcat tgtattttct cctcttttta
    16021 ctaccaaata gctaattccg actgttctaa tcgaagtaag ccttcaataa aaaaataatc
    16081 agaatagatc agtggcacat tataagtcag ttctctatgg tatgcttctg ctccgcctcc
    16141 aatgatccca tcttcatcca tattccaatt tgcatagtgc ttttctgtct tttggagtat
    16201 attgatagca gcttcatgat acatttttga tcgtacagag gaaacatatc ctgctaattc
    16261 cagaagaccg catgcagcgc atactccagc actggtatca atattcgaat cttttacagc
    16321 aaaatcaatg agaggaatat aaccagtttt ttcgattttc tgaataaaat ttactgctat
    16381 cttttctgct atagctagat agtacggatt ttttgtgtaa cgataactta acgcgaatcc
    16441 atataaagcc catgcatttc ctctgctcca acaagaattc tcattgtatc cttgcccgcc
    16501 aagtaaccga atatatttcc ctgattctgc atcaaattct gcgatatgcc cggttgatcc
    16561 atctttcctc actagatact tagctgttgt atctgcatgc tgccgagcaa ttttagagaa
    16621 cctcggatca ccagtttccc tcgaagccca atacagtaat ggtaaattca tcatactatc
    16681 aataatcatc caaccaggtt tatggtcatt ccatgctact aaatagttcc ctgaaatatt
    16741 atagcgtcca gcaagtaccg tagctgcatg taaccctagt tgtcgactat gtttgttttg
    16801 agactgttga taatgtgcaa tagccgatgg taagaataaa aaacctacat catgatgaac
    16861 gtctgaaaaa tggtacaggg cagattctaa taattgttct tgttgtattg ctgcctcttt
    16921 gaacttttct cgttttgtct tgtgaaagag ctgccataaa ataccactcc aaaaaccatt
    16981 ggtccaccag tcgattcctc tgtcctttat tacgtctgta tattttccgt tgtaagaaat
    17041 atatggaatt ttattcccca ttctgtctaa ttccgcatct atcttttcag aagctacatt
    17101 caaaattttt cttgaccatt ccttcgtctg ttcattcatg gatatcccgc cattctttta
    17161 atttttatga atgtggagtt gctcctagaa cgaactagat ggttaacatt cgttattttt
    17221 tttacttatt tgcagctcca atgtcattct ccttaaattt agactgcgcc cacacataaa
    17281 tcaagaccat tgggatcact gaagttgcta aaattagcat aattgcgcca taattaattg
    17341 gctcacctgt actagaaacc gtcatatttt tgataattaa gggaaccgta tatttatctt
    17401 tatctgaaag catgactaat ggaaatagat attgattcca acttcccata aagataagta
    17461 aaccactagt aataatcgta ggtgataaaa tcggtacagc gattttcgta aaaatcaccc
    17521 actcattggc accatcgatt ctggcagcct ccatcagtgc tgtaggaaat tgactgctat
    17581 attgtcgcat cataaacact gaagttgtac tggcaatcga agggatgatc acacctgcat
    17641 aagtattcga taatcctaaa cgactcataa tgtaaaataa aggaatcaat gtggtaaaaa
    17701 aaggcaccat cctagatagc ataactgtat tgaacaacag tttattcccc ataaaacgat
    17761 actttgctaa tgcataaccc gccattgtag aactggctgt agctgaaact gtactgatta
    17821 cagcaataac aatcgaattc caaataatca agggataatt atatctcgcc atgatggctt
    17881 gccagttttt taatagcata tccccaaact tgaaattgat tccgccactt aaaatttctt
    17941 gattactttg cgttgaagct acaatcatat ataaatatgg catcaaaaat attaaggcac
    18001 caaacacaat aatgacgtac gtaaaaattc tgctttgtat cttttgttta gtcattttta
    18061 tcacctccga atttaaattg aatagccgat aatataacag atacaaacac catcgtccag
    18121 gcaatagcag aggcttttcc aaaatcaacc atttcaaaac ttgttttata taaattgact
    18181 cctacgacca aagctgaatt ttctggtcca ttggaagtcc caaagaatat atttgggata
    18241 ttgaaattac ccaagccatt gatagtggct aaaactgtgg ccattaaaat gattggcttt
    18301 agcatgggga tcgttaataa aaagaattgc cgaaatttcg atgctccatc tatttcagca
    18361 ctttcgtata tctctttaga aatcccttga agaccagcta aaaataaaat agtgttatat
    18421 cctacactaa gccaaatcat cgtgatgatc attgatattc gtatccaaaa aggatttgtt
    18481 aaccactgta caccgttaat tcctagcgca ttcaaaaatc cattgaataa cccagtcgga
    18541 ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg atgtaatggt tggaatataa
    18601 tagacagttc taaaaaatcc ttttcctttt aactgtgctg aattcaataa aacagcaaag
    18661 taaatagcta atagcatcgt tattggaatt gatcctgcca tgataatcaa aacattcgtg
    18721 taagatttaa taaacaatgg atctcggaag atagctagat aattgtcaaa tccaacaaat
    18781 tcaaatgagt agccaatttt cataaaacta aaagcaaacg agataatgat gggaatcaaa
    18841 atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc cataagcact ttttttatga
    18901 gacgtatctg ttgctttttt cttgctgttc tttattgtct tgcttggtaa cgaagattgc
    18961 atcatcgtta agtcctccta atatttttta atcaccagtt aattttacgc cgtacttatg
    19021 agcaaaattt tctgcttctt ttagatatga attttcaaaa tcacctgttt gccaatagtt
    19081 atatgcagcg gtagtcaaat aagacgtagc atctgaataa gtggctggta aaacagtatt
    19141 tgatgtattt tcattgattt tattcaatag ttcatagtat ttttgtcctc cttagtagtc
    19201 aaactttttc tcagctgctt ctgtttgata ggccaatgag ttagatgtag ccacgcccag
    19261 ctccagtgcg ttggctaagc attcttcatc tgtcagcata aatgtaagaa attgttgtgc
    19321 cacttgtgga tttttggcat ctttagggac ataaaaactt gatcctcctg atactgcacc
    19381 ttttcctgga ttttcttttg aaaaaggaat cagtggagcc atgcgccatt tgtcagatgc
    19441 atcaggaaaa ttcaaagaca tattcgtagc ataccagccg ccagcaacaa acattgcaga
    19501 ttcttgaaat gctttctcag catcttgtgc accgtagaaa tgtacaatat ctgagtcgat
    19561 catttctttg attaactttg cagcatgttt cgcttcttta gtattcagac ggatatttcc
    19621 ttcttcatca agcaaagatt gattctgctg ggctagcatg ttgataaaca attcttggtc
    19681 cccagattct ggcaaggcaa ttccaaaact accagttttt tcttttaatg cttttgccac
    19741 ctggatatat tcttcccaag aactgatttc ttcaaaatct acacctattt cagcaaattt
    19801 ttttggttga taataacaaa gtgctggggt aaaatcacta gcccaaggaa taatcgtttc
    19861 attactcgtt tgttgacgca aaatattcaa tttttgatca taaaactgat cgccatattt
    19921 gtcataaaat ccatattcat cagcactata aaaagcatct tgaaattttt ccagcacacc
    19981 tgctgctgaa gtgtctgcca tggagataat atccggcata tcttcgtgtg ttaccaattt
    20041 tggggtgatc atttgtactg ttgctgttcc cgaagctaca ttcacaattt ttaagtcaat
    20101 atttgctcca gttttgcggt tatagatttt tgcagcattt tcaacatagt ttgtcagttt
    20161 gccgtttgcg acatatactt ctaatgtagt ggcttttttt atcattggat ctgcgatgtt
    20221 tgctgttact tcttcgttag tttttccaca tgcacttaag ctgaataata gggtaatgtt
    20281 tagcaaagct atcaccaatt tgtatctttt tttcatgtct atcccttctt tcttttcaag
    20341 ttcttatata aaacgctttc acttatgtat attaacggaa ttagtaacac aaatatttat
    20401 ataaaaaaat attttacttg tcgttttggc ttttcatttt tgttataata gtcattaatt
    20461 ataaatagaa agctttttat ataaaaagag aaaggaaagt ggttcttttg ttaacaaaca
    20521 accaatcctc caatcttgtc aaaaaaacac taacctattc catagatacc catcttcatt
    20581 tatctgctgc tccctatttt cgactactaa taaatgaaag cgctaattta tatgctgaat
    20641 ttgcacataa atctatagaa ctagataatc cttccctttt attcattgat aaacagcaag
    20701 aagtccaaat cttttctact caaaaggaaa cacaaacttt tatcattatc cagttttatt
    20761 ttgatgagca attattagca catattacgg gacaatcttt ttctatagat caagaaataa
    20821 catctatcat tacttttctg aaaaatgaac agttcaaaat tgattatcta aaacatagta
    20881 agccagctat ttcctatgat caggaaatca attctttttt catactatgc caacagatga
    20941 gcattcattt gctttcctta ttagttactt attgtctgca aaaaattcaa ttgtcttcct
    21001 taccaaggca ttatcaacag gaacttgctt tattgacaat accttcaaat gagaaagtca
    21061 ttcctaaaaa aaagcatatt ctttattcag atgaattagt tgatccaatc atacgatatt
    21121 tagaagaaca tctttcagaa aattttttgc ttgaacaatt agcccaaatt ttttttatca
    21181 gtccagccag cttaaaaaaa cattttagaa aagtgaatgg aaaaagtgtt attagctatt
    21241 ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct agaaaatgaa ttaacctata
    21301 cagaaattgc agatatatta ggatttcatt cgatccatca tttttcttca gcttttaaaa
    21361 aatatacagg gttatcccct tcaa
//
LOCUS       DACCWK010000039        21327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCWK010000039 DACCWK010000000
VERSION     DACCWK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.039"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            315..623
                     /gene="rpsJ"
                     /locus_tag="IVW65_001405"
     CDS             315..623
                     /gene="rpsJ"
                     /locus_tag="IVW65_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7900288.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            654..1283
                     /gene="rplC"
                     /locus_tag="IVW65_001406"
     CDS             654..1283
                     /gene="rplC"
                     /locus_tag="IVW65_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7900289.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1311..1934
                     /gene="rplD"
                     /locus_tag="IVW65_001407"
     CDS             1311..1934
                     /gene="rplD"
                     /locus_tag="IVW65_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7900290.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1934..2224
                     /gene="rplW"
                     /locus_tag="IVW65_001408"
     CDS             1934..2224
                     /gene="rplW"
                     /locus_tag="IVW65_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7900291.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2263..3096
                     /gene="rplB"
                     /locus_tag="IVW65_001409"
     CDS             2263..3096
                     /gene="rplB"
                     /locus_tag="IVW65_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7900292.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3139..3417
                     /gene="rpsS"
                     /locus_tag="IVW65_001410"
     CDS             3139..3417
                     /gene="rpsS"
                     /locus_tag="IVW65_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7900293.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3440..3787
                     /gene="rplV"
                     /locus_tag="IVW65_001411"
     CDS             3440..3787
                     /gene="rplV"
                     /locus_tag="IVW65_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7900294.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3801..4457
                     /gene="rpsC"
                     /locus_tag="IVW65_001412"
     CDS             3801..4457
                     /gene="rpsC"
                     /locus_tag="IVW65_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7900295.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4460..4894
                     /gene="rplP"
                     /locus_tag="IVW65_001413"
     CDS             4460..4894
                     /gene="rplP"
                     /locus_tag="IVW65_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7900296.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4884..5072
                     /gene="rpmC"
                     /locus_tag="IVW65_001414"
     CDS             4884..5072
                     /gene="rpmC"
                     /locus_tag="IVW65_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7900297.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5097..5363
                     /gene="rpsQ"
                     /locus_tag="IVW65_001415"
     CDS             5097..5363
                     /gene="rpsQ"
                     /locus_tag="IVW65_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7900298.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5421..5789
                     /gene="rplN"
                     /locus_tag="IVW65_001416"
     CDS             5421..5789
                     /gene="rplN"
                     /locus_tag="IVW65_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7900299.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5826..6134
                     /gene="rplX"
                     /locus_tag="IVW65_001417"
     CDS             5826..6134
                     /gene="rplX"
                     /locus_tag="IVW65_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7900300.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6162..6701
                     /gene="rplE"
                     /locus_tag="IVW65_001418"
     CDS             6162..6701
                     /gene="rplE"
                     /locus_tag="IVW65_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7900301.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6720..6905
                     /locus_tag="IVW65_001419"
     CDS             6720..6905
                     /locus_tag="IVW65_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7900302.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6941..7339
                     /gene="rpsH"
                     /locus_tag="IVW65_001420"
     CDS             6941..7339
                     /gene="rpsH"
                     /locus_tag="IVW65_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7900303.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7371..7907
                     /gene="rplF"
                     /locus_tag="IVW65_001421"
     CDS             7371..7907
                     /gene="rplF"
                     /locus_tag="IVW65_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7900304.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8068..8424
                     /gene="rplR"
                     /locus_tag="IVW65_001422"
     CDS             8068..8424
                     /gene="rplR"
                     /locus_tag="IVW65_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7900305.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8445..8945
                     /gene="rpsE"
                     /locus_tag="IVW65_001423"
     CDS             8445..8945
                     /gene="rpsE"
                     /locus_tag="IVW65_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7900306.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8960..9139
                     /gene="rpmD"
                     /locus_tag="IVW65_001424"
     CDS             8960..9139
                     /gene="rpmD"
                     /locus_tag="IVW65_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7900307.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9183..9623
                     /gene="rplO"
                     /locus_tag="IVW65_001425"
     CDS             9183..9623
                     /gene="rplO"
                     /locus_tag="IVW65_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7900308.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9623..10918
                     /gene="secY"
                     /locus_tag="IVW65_001426"
     CDS             9623..10918
                     /gene="secY"
                     /locus_tag="IVW65_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7900309.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10980..11627
                     /locus_tag="IVW65_001427"
     CDS             10980..11627
                     /locus_tag="IVW65_001427"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7900310.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11818..12036
                     /gene="infA"
                     /locus_tag="IVW65_001428"
     CDS             11818..12036
                     /gene="infA"
                     /locus_tag="IVW65_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7900311.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12069..12185
                     /gene="rpmJ"
                     /locus_tag="IVW65_001429"
     CDS             12069..12185
                     /gene="rpmJ"
                     /locus_tag="IVW65_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7900312.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12203..12568
                     /gene="rpsM"
                     /locus_tag="IVW65_001430"
     CDS             12203..12568
                     /gene="rpsM"
                     /locus_tag="IVW65_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7900313.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12596..12985
                     /gene="rpsK"
                     /locus_tag="IVW65_001431"
     CDS             12596..12985
                     /gene="rpsK"
                     /locus_tag="IVW65_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7900314.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13065..14003
                     /locus_tag="IVW65_001432"
     CDS             13065..14003
                     /locus_tag="IVW65_001432"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7900315.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14031..14411
                     /gene="rplQ"
                     /locus_tag="IVW65_001433"
     CDS             14031..14411
                     /gene="rplQ"
                     /locus_tag="IVW65_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7900316.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14683..15522
                     /locus_tag="IVW65_001434"
     CDS             14683..15522
                     /locus_tag="IVW65_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7900317.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15498..16367
                     /locus_tag="IVW65_001435"
     CDS             15498..16367
                     /locus_tag="IVW65_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7900318.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16357..17154
                     /locus_tag="IVW65_001436"
     CDS             16357..17154
                     /locus_tag="IVW65_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7900319.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17357..18103
                     /gene="truA"
                     /locus_tag="IVW65_001437"
     CDS             17357..18103
                     /gene="truA"
                     /locus_tag="IVW65_001437"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7900320.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18558..21227)
                     /locus_tag="IVW65_001438"
     CDS             complement(18558..21227)
                     /locus_tag="IVW65_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7900321.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         7093 a         3889 c         4600 g         5745 t
ORIGIN      
        1 ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga aataaaagag
       61 tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg
      121 ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg aattgcacag
      181 acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc ccggacgctt
      241 tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc aaaataggcg
      301 aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc
      361 gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact ggagctagcg
      421 tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc cgtgcgactc
      481 ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt cttatcgaca
      541 ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta ccatctggtg
      601 taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta ctcatgacca
      661 aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct ggtgaattaa
      721 ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa acaatggaaa
      781 cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt ttatcaaaca
      841 aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc attcgtgaat
      901 tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta gatgttttcc
      961 aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc caaggggtta
     1021 tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc
     1081 ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc
     1141 gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc gatgcagaaa
     1201 gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta attactatca
     1261 aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga atgccgaatg
     1321 tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac gaagaaatct
     1381 tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat
     1441 taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac
     1501 catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc
     1561 gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa cttcctaaaa
     1621 aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg
     1681 tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa caagttcttg
     1741 ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac gactttgctg
     1801 ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc
     1861 tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact caaattgagg
     1921 aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc
     1981 aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg caaacaaaac
     2041 tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt
     2101 gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa aaaaacgtcg
     2161 caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg aagctgctga
     2221 ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat taaaaagtac
     2281 aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga aatcacaaca
     2341 tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg taacaacaac
     2401 ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac
     2461 ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata tgatccaaac
     2521 cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta catcttagca
     2581 cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc
     2641 ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa cattgaaatg
     2701 aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca agtacttggt
     2761 aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat gatcttagct
     2821 gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat caacatcggt
     2881 aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac
     2941 cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg taaagcacca
     3001 gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa agctaaatca
     3061 gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg tgtcgcacat
     3121 tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat
     3181 ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg
     3241 tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt ttatgatgga
     3301 cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt aggtgaattt
     3361 gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa acgctaattt
     3421 gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac
     3481 agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag gtaaaagtgt
     3541 cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa tcattgaaaa
     3601 agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg ttgaaaactt
     3661 ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc
     3721 aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag tcgtatcaga
     3781 aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca
     3841 tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg
     3901 aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct gtatcaacag
     3961 ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct aaaccaggta
     4021 tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta aacaaattaa
     4081 ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat
     4141 tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa
     4201 aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact caagtatctg
     4261 gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga actgttcctc
     4321 ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca acatacggaa
     4381 aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa aaaaacactg
     4441 agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc gtgaattccg
     4501 cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg aatacggttt
     4561 gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat
     4621 gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc acaaatctta
     4681 tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg aaggatgggt
     4741 tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc
     4801 tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga tcgtaaaacg
     4861 tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta accactgccg
     4921 aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga ttccaattag
     4981 caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg attgcacgca
     5041 tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt aagcgtatga
     5101 ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac aaaatggata
     5161 aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc aaacgcatga
     5221 actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg
     5281 tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg ttggaagtag
     5341 tcgaagaagc agttattatc taataacacg agattttcct atatagattg aatgcaaaat
     5401 ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc gctgataact
     5461 caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt aaaactgcta
     5521 acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg gttgtcaaaa
     5581 aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt cgtacagacg
     5641 gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat aaaagccctc
     5701 gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga
     5761 tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg aggtgcgaaa
     5821 cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa gacaaaaaca
     5881 aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc gaaggtgtaa
     5941 atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga attgttgaga
     6001 tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt gtagctggcc
     6061 gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag
     6121 ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa
     6181 aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc tctgttatgc
     6241 aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg
     6301 ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa aaaccaatga
     6361 ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg ccaattggtg
     6421 caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg gtttctgttt
     6481 cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa
     6541 actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat gatttagtag
     6601 ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat gaagaatctc
     6661 gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag gcgaactacg
     6721 tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca acacaagctt
     6781 atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc catctttgcc
     6841 gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag aaagctagct
     6901 ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga cagatccaat
     6961 tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt ctttagaagt
     7021 acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg
     7081 tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg
     7141 taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc
     7201 ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg caatcatttc
     7261 gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt
     7321 tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa gtgagccgta
     7381 tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac ggaaacaacg
     7441 ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat atcaaaatga
     7501 atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa atgaaaacaa
     7561 ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc gaaggtttcc
     7621 aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga tcaaaacttg
     7681 tattgaacgt tggttactca catccagtag aaatcacacc accagctgga gtaactgttg
     7741 aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat
     7801 tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa ggtattcgtt
     7861 atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa
     7921 ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt caaaaaaata
     7981 catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt gaaatagtag
     8041 tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa caaaacacgt
     8101 caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg cccacgcttg
     8161 aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt agcgggtgta
     8221 acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa aacagaagca
     8281 gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat taaagtagta
     8341 gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc tgaagctgct
     8401 cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt tatatcgatc
     8461 caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta
     8521 aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa aacggacacg
     8581 taggatttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag
     8641 atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca cacgaagtaa
     8701 tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt tctggggttg
     8761 ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat atcacttcta
     8821 aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac
     8881 aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg
     8941 gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca gtattatcgg
     9001 acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga ttaacagttc
     9061 agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt ctcacctagt
     9121 ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga ggtgccaaac
     9181 ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg
     9241 gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag
     9301 ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca ttgttccgtc
     9361 gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc attaatcttg
     9421 atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta gttgaagctg
     9481 gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa ctaaacaaga
     9541 aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt gaagcggctg
     9601 gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta aagtaaaaga
     9661 cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca
     9721 tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt tgccattttt
     9781 caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt tctcaatggg
     9841 ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg acatcgtgcc
     9901 tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga atcaagcgac
     9961 tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg ctggattcaa
    10021 tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg taacgatcgg
    10081 tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga tcacagaaaa
    10141 aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta gattgcctga
    10201 aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag acatatggaa
    10261 atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga cattcgtaac
    10321 atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg tagctggcgc
    10381 gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc cagttatctt
    10441 tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg gtagattcgg
    10501 ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag cacctggtgc
    10561 tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa
    10621 ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc
    10681 tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta cagttggtgc
    10741 agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt gggatcttcc
    10801 acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag ctttagaaac
    10861 tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca ttgagtaaga
    10921 aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga gggaaaacaa
    10981 tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct gaaaaaatca
    11041 ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca gcgatgcaaa
    11101 acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca ttagttcctg
    11161 atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca gagaaaggct
    11221 tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac gcaatgctaa
    11281 aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg
    11341 tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac cataaaattt
    11401 tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa
    11461 gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc aaaaatagtg
    11521 aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat ggtgctcgtg
    11581 aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac gattgcccgt
    11641 agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat agaatatgtc
    11701 agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag gtgggaactg
    11761 cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg
    11821 gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg
    11881 tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg taaaattcgt
    11941 atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc tccatacgat
    12001 cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa actacaggag
    12061 gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc
    12121 gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag
    12181 gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc ctcgtgataa
    12241 acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg ctaaaaagat
    12301 cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac
    12361 agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt
    12421 gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc gtcatcgccg
    12481 tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta aaggcccagc
    12541 tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa acttcatggc
    12601 agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag gtattgcaca
    12661 tatccattct acattcaata acactatcat catgatcact gatactcacg gaaatgcatt
    12721 agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa ctccttttgc
    12781 cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat taaagactgt
    12841 tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt cattacaagc
    12901 tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata atggtgcccg
    12961 ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta tcagtaacgt
    13021 cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt gagttcgaaa
    13081 aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc gttgaacctc
    13141 ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta ttatcttctt
    13201 tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa ttctctacca
    13261 tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga
    13321 aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct gctacagtaa
    13381 ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat atgtacatct
    13441 gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct ggacgtggtt
    13501 atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt ccagtcgatt
    13561 caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt gttggacacc
    13621 gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt gaaccgttgg
    13681 aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt gtgaacttaa
    13741 ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa
    13801 tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat tgtttgaaac
    13861 gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa atgatcaaag
    13921 tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac gacttaggtt
    13981 taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac gtgagttatc
    14041 gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta acaactgatt
    14101 tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt cgctcaactg
    14161 cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg
    14221 ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat
    14281 cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt caaggcggat
    14341 acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag
    14401 aacttgtcta aaatcacact aataacatca ctttatagat gattctttta gagtgttatg
    14461 atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga atatcgcttg
    14521 gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt catgacagat
    14581 atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt
    14641 tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa
    14701 ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc
    14761 tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa
    14821 tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg
    14881 ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt tggaatggtt
    14941 tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt
    15001 cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag
    15061 aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa
    15121 caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa
    15181 gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc
    15241 aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat
    15301 gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa
    15361 attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa
    15421 ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa
    15481 aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga
    15541 atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt
    15601 atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg
    15661 cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata
    15721 cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag
    15781 aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg
    15841 gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg
    15901 atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag
    15961 caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag
    16021 gacttgatcc ccagggcaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac
    16081 agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt
    16141 atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc
    16201 aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag
    16261 aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag
    16321 cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt
    16381 cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc
    16441 agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg
    16501 gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga
    16561 ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca
    16621 agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg
    16681 aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg
    16741 cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa
    16801 gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg
    16861 cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat
    16921 tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta
    16981 tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac
    17041 aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg
    17101 gttatcattg cttttgtatt attaactgtg ctactgatct ttttaagagg ataaacgttc
    17161 agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat gcaagcagtg
    17221 tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct
    17281 gaaaagaaag aacgaaaaaa ttttatagaa aaatcattat tccagataac cttttaggaa
    17341 caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg gaacaaattt
    17401 taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag aaaaaacatt
    17461 acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga cagatgccgg
    17521 agtacatgcg atcgggcaag taatccattt tgattatcca gaggagcgtc cgttagaacg
    17581 aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac aagtagaaat
    17641 tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc aatttcgagt
    17701 ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt ttccttaccc
    17761 acttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc acgatttcac
    17821 ctctttttgt gcatcaggaa gctcgataga ggataaaata cgtacgatct acgaggctaa
    17881 gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa atggcttctt
    17941 gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg gccgattgcc
    18001 tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag gacctacagc
    18061 acaccctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggtta tgatagaagt
    18121 ggtcagcttc aagaaataag aaggtattta cgaaaattac ttctcaaaat tttgcaaaat
    18181 ttcggcttat tttcgaagaa gctacttctg gaacaccgtt taaggaagag gtcgtgaaat
    18241 cagcgttttg acctgagtat tagagaaaca aacgggaaaa tagctcctca tattttgccg
    18301 tttgttgagc taataccgaa ggtcagctga ttgaacaccg tttaagaaag aggtcgtgag
    18361 cctgtcattt aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat
    18421 gaatatcgtg acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt
    18481 ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa
    18541 caggttccat cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct
    18601 tctattgctg gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa
    18661 agaataaaca atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc
    18721 aagtaactga aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct
    18781 ttgaaattgc tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa
    18841 ggaatagttg tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg
    18901 ccagtggctt ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct
    18961 acaacatcta gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa
    19021 cttgcagtaa atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca
    19081 gaaatgttga tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt
    19141 tctaattgcg caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca
    19201 tggacttccc aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca
    19261 gtcgttgtct ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta
    19321 aaatactgtt catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta
    19381 tctgtcactg gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat
    19441 gcattgacat tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat
    19501 ttactgaacg tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc
    19561 agcaaagtat tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat
    19621 ttatccaggt aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg
    19681 taaccatagt tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac
    19741 gagtcggaat ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta
    19801 tataaactgc gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta
    19861 ttgaccagtg cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa
    19921 agttttttat gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc
    19981 aatacaaagg aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc
    20041 accgcaaaga gagcagctac gaccaaaatg attcctaaca acagcccacg atcgtcttgt
    20101 tctatctttt cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca
    20161 taacggaata aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca
    20221 agataaaaac ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac
    20281 ggtactttcc gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa
    20341 acgaacggaa tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt
    20401 agatccagat aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca
    20461 tttgaccgaa gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt
    20521 aaagaagtga taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga
    20581 cgggcgataa aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat
    20641 aaaaacaaga gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga
    20701 tgaatcccca aaatcactaa tggaagataa aaaaatgcat ccagccacat gatgatctcc
    20761 gaatgtgcag tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag
    20821 cgaggaatct tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta
    20881 agcaaagtca gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt
    20941 ggcgtgaaga tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa
    21001 gcattccatg tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga
    21061 aaattagaaa actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag
    21121 attccgatac ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct
    21181 gcgtagcgtc cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac
    21241 caaatttcta tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga
    21301 gtctagtttt cacaaaaaaa taaaatt
//
LOCUS       DACCWK010000040        21214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCWK010000040 DACCWK010000000
VERSION     DACCWK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.040"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(28..741)
                     /locus_tag="IVW65_001439"
     CDS             complement(28..741)
                     /locus_tag="IVW65_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7900322.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            916..2340
                     /locus_tag="IVW65_001440"
     CDS             916..2340
                     /locus_tag="IVW65_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7900323.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2639..3346
                     /locus_tag="IVW65_001441"
     CDS             2639..3346
                     /locus_tag="IVW65_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7900324.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3333..3656
                     /locus_tag="IVW65_001442"
     CDS             3333..3656
                     /locus_tag="IVW65_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7900325.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3745..4782)
                     /locus_tag="IVW65_001443"
     CDS             complement(3745..4782)
                     /locus_tag="IVW65_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7900326.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5103..6086)
                     /locus_tag="IVW65_001444"
     CDS             complement(5103..6086)
                     /locus_tag="IVW65_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7900327.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6501..7271)
                     /locus_tag="IVW65_001445"
     CDS             complement(6501..7271)
                     /locus_tag="IVW65_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7900328.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7264..8199)
                     /locus_tag="IVW65_001446"
     CDS             complement(7264..8199)
                     /locus_tag="IVW65_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900329.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8515..10164)
                     /locus_tag="IVW65_001447"
     CDS             complement(8515..10164)
                     /locus_tag="IVW65_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7900330.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10281..12884)
                     /locus_tag="IVW65_001448"
     CDS             complement(10281..12884)
                     /locus_tag="IVW65_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7900331.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12896..13945)
                     /locus_tag="IVW65_001449"
     CDS             complement(12896..13945)
                     /locus_tag="IVW65_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP7900332.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13971..14285)
                     /locus_tag="IVW65_001450"
     CDS             complement(13971..14285)
                     /locus_tag="IVW65_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7900333.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14301..14747)
                     /locus_tag="IVW65_001451"
     CDS             complement(14301..14747)
                     /locus_tag="IVW65_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7900334.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14748..16724)
                     /locus_tag="IVW65_001452"
     CDS             complement(14748..16724)
                     /locus_tag="IVW65_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7900335.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(16882..17778)
                     /locus_tag="IVW65_001453"
     CDS             complement(16882..17778)
                     /locus_tag="IVW65_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7900336.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(17771..18451)
                     /locus_tag="IVW65_001454"
     CDS             complement(17771..18451)
                     /locus_tag="IVW65_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900337.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(18452..19234)
                     /locus_tag="IVW65_001455"
     CDS             complement(18452..19234)
                     /locus_tag="IVW65_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7900338.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19227..20144)
                     /locus_tag="IVW65_001456"
     CDS             complement(19227..20144)
                     /locus_tag="IVW65_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7900339.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20308..20631)
                     /locus_tag="IVW65_001457"
     CDS             complement(20308..20631)
                     /locus_tag="IVW65_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7900340.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         6285 a         4371 c         3527 g         7031 t
ORIGIN      
        1 agctctgaaa aaataatttt cccgagctta tttttcaaca aaaacagctt caaaaacaaa
       61 gtcttgatat aaatgtctcg ataccgaata ctcgaaaggt ttaccagacg atagagcaac
      121 aactttgtct atctcaatca taaattcacc tggatctatt tttaatattt ttgcgtcaaa
      181 ttcattcgca gtatctcctt tgatacgtat gtgcgaagtc tttatttcca gacccagttc
      241 atttgtgata taagcatata cagatttctt taaatgtttt ggttccaagc ctggtatgac
      301 tgctaatggc atgaatgttt gctcaatcga gtatggttga caatcaataa aacggcttcg
      361 tacaatatta tatacatagt cttttacact gatacttaat ttatcagcta cttttttcga
      421 tggaagttca attgagaaag tatggatttc tgaatctacg tgttcttcac caaccacgct
      481 aaacaaacct tcgtttggac caagaactcg tgagttatca attggtgttc tgacgacttc
      541 agtcccatac ccacttctac ttcgcaaaac tccttcggaa acaagtaagt ctacgccttt
      601 ctttactgtt aacttgcttg tatgataaat aactgccaat tcatctcctg taggaagttt
      661 ctcaccaatt ttatactctc catttacaat ttttttcttt atatctaagt aaatttccat
      721 atacttcgca atttttttca atggtgaccc tccaatcaaa aacatatctt catctatact
      781 ttaacataaa taaaccgata aaatccacat caaaattata cacacaataa aaaagatata
      841 tatatttaaa caaaactatt tactttaagt tgtaagcggt gttatcatta agtggaaaca
      901 ggaggtaaaa aaattatgac aactatgaaa aagctaactt ttccagatga tttttggtgg
      961 ggttccgctt ggtcagctga gcaagcagaa ggaaaaggag aaacaggaaa agcagagact
     1021 atctgggatc gctggtttaa agaacaacct taccggtttt atcaacgaat cgattcctct
     1081 attacaaccg atcacatcca tcaatataaa gaagatatag aactaatgaa aaaaactggg
     1141 cataactcct ttcgcgtttc tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga
     1201 aaagtaaatc ccaaagccat ttctttctat cgaaaattat tttccgaaat gaataataac
     1261 ggaatcaaag tatttgccaa cttatatcat tttgacatgc ctgctactct tcaagataaa
     1321 ggaggatggg aatcacgtga agtagttgat gcttatgttc attacgctga aacttgtttt
     1381 aaagaatttg gtgacttagt ttgtcattgg tttaccttca atgaaccact tgggcctatt
     1441 ttaggtactt atcttgaaga ttttcattat ccaaacctca ttgactttaa acgcggagcg
     1501 caagcagcat tcttcactat acttgcacac gcaaaagcaa tacaagtatt taaagaacta
     1561 gctcttccta gtaaaatagg cgttattcta aatttgagtc ctacttttcc tcgtagtcaa
     1621 aatcctgctg atgtgaaagc agcagaaatc gctgatttat tttatacgcg gagcttttta
     1681 gatcctatgg ttaaagggaa tttttctaaa aagcttatcc tgttattaca agaatataat
     1741 cagcttcctg aagattatac gaaagaagat cttgtatgta tctacgaaaa taccgctcaa
     1801 attcttggac taaactatta cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat
     1861 acatctagtc catttttacc agagtggttt ttcgaaccac atatcatgcc tggacgacga
     1921 atgaacgaat accggggatg ggaaatttac gaaaaaggaa tttatgattt gtgcatggat
     1981 atcaagaacc attatggaaa catcgaagca tttatctcgg agaacggaat gggcgttgcg
     2041 gatgaagaac gtttcatgga tgatacaggg caaataattg atgattatcg gattgagtat
     2101 atcaaagatc atcttgctta cttatggaag gcaataaatg aaggatgtaa cattaagggg
     2161 tatcatcttt ggacatttat tgattgctgg tcatggatca acgcctataa aaaccgatat
     2221 gggctggttt cactcgacct tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt
     2281 tacaagcaat taagtgatac aaatggattt agatacgatg tagacttgtt agttgaataa
     2341 tattctaaaa ttccacttga agtgaacaca ttagaaaaca agaataatga gaaataccaa
     2401 taacaaatac tttttcttca tacttcattt atacttgtca gtataagaga ctgtgacaaa
     2461 tgtagtgatc ctcagaagtt aggtctggaa atctaacttg agaagatttt tttaaactac
     2521 gtccagtctt ttttatttgt ttaaaattga agttgacaag tcttcattaa aaatttagaa
     2581 tattcacaag tttgacgaca attatgaaaa tttatgtcgt gctaaaggag ttttgttatt
     2641 ggatgaaaag ttagatataa aaactgcgat caaagacacg ttacctaccg tttttggtta
     2701 tatcggtatt ggacttgcat ttggtatcgt tgggaaagct gccgggtttc atccgctggt
     2761 cgtcacgttg atgtccctgc tgatctatgc tggttctgcc caatttatca cggtcagcat
     2821 gcttgctagc catagtccac tgctctccat cgttctctcg acctttctag tcaattcccg
     2881 aatgattctg atgagtatga cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa
     2941 tctgctgatt ggcacattgc tgacagatga aagtttcgca ctgggaatga ataaattgaa
     3001 ctatactgga caaaagttaa actttcgttg gatgaataca gctaattgga tttcttattt
     3061 gacgtgggtg ggttcttctc ttgttggcgc acttttaggg aattttatta ctgatccgaa
     3121 aaagtttggt ttggatttcg ccatcgtcgc tatgtttatc gggttacttt atcttcaagt
     3181 catttctgat agaaacacct ctaaacgatt acagttgatc ctgattggtc tcactttgat
     3241 tctggtatat gtcggactga tttttatccc aagtaactta gtgattttag ttgtaacact
     3301 gattgggtgc ggattggggg tgtggatcaa acatgccttc ttttgagttt acattgctga
     3361 ctattttagg ttgtacttta gcaacttggc tttctaggat cctaccattt gtcttactga
     3421 aaaaattcga tttgccgcaa ccgttgcttg agtatttaag ttttgtcccg atcgtcatta
     3481 tgtctgcttt atggttcagc agtctattta cccaaaatat cggacatctg ccgcagatca
     3541 atctcgaaaa tgctttggct tcagttccta cacttttagc tgctattctt tcaaaaagct
     3601 tattggtgat cgtcttggta ggaattcttt ccctttcgtt gattcgctta ttataaataa
     3661 aaaaacaaga gcttatggca aattactgtc atgcgctctt gtttttatca aagtcaaata
     3721 agaaaaaaat cggcgtattc ttgcttatcc gttgaacgga ccactaacaa cgacttttcc
     3781 taatatatgt cctgtttcta cgtctttggt tgcttgtttc aagttcttcg cattcatccc
     3841 atctgaatag accttgctca atgtactgcg gataatccct tcatttgcta aagcagcaat
     3901 gtgcgctaaa gcttctcctt gcgtttcgat ttcatagtta tagtctgttt tagcgaacat
     3961 atactcccag tcgaagctga tagattggtt tttccaaaca cctatatcta acggctcttt
     4021 gatccctacg atcgttccca catgtccaaa tggtcggaca aggtgtttga tttgatcaaa
     4081 gtagcgggta atatcgaata atacagcgac atactctact tgatcgattc ctaattcacg
     4141 aaaactttta cttaagtcgt gatgataatc gattggatag tctacacctg tttttttcag
     4201 ccattcaaaa ttcttcggac ttgcagtcgc gtagacttct aggccagccc aatgcgctag
     4261 ttgattcaaa atcgaaccga cgcccccagc tccgttgatc actaagattt ttttcccgcg
     4321 gttggcattt tcttctggag tcagaccaaa tttttcaaat aacaattcat aagcggtcaa
     4381 tgaggtcaac ggcaaggctg cggcttcttc atctgaaaga ttttttggtg caagagcgac
     4441 aatacgttcg tcaaccagtt ggtattcttg attgcttcct gctcttgtcg ttgtacctgc
     4501 gtaaaaaacg cgatcgccga cagaaaattt ttgcacttca tcacctacag caacaacttt
     4561 cccgactcca tcaaacccta agatacgtaa agcatttctt atcccgttat cttggcgaag
     4621 tttcgtatcg acaggattaa cagatactgc gttgactttt accagaagat cccgtgaact
     4681 gggtacaggt atctgttctt tttcatcaat aaagcttttt ggatcatcga tcgacaaccc
     4741 ttcataaaaa ccgactacct tcattaattc cttttctgac atacgaattc ctccttagct
     4801 tcatcaagtt ggagcatcca tacagttttc atttcaatat atatagtatg ctatttgttc
     4861 cacttttatt catcataatt attctcttac ttaaagtaaa ctatttgcac ttttttggtt
     4921 aaaaaagagg ttgcgacaag acttttgtca caacctcttt taaacggtgt tcaatcaact
     4981 gaccctcggt attcgctcaa aaaacgacaa aatatgagga gctatttttg tttggctgtt
     5041 cttattactt gatgcaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga
     5101 aatcattgtt cactgaaaag cgttggatgt tctttttcaa aggtgtctaa gatctctcgt
     5161 acttcatcag tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat
     5221 cctttgatat agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct
     5281 gaataatgat cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta
     5341 tgttccctcg gttctttttc aaatgcataa ggattcttga aaatcccccg gccaatcatg
     5401 atgccatcta cgccatattt ttcggccaat tcttctcctg tctggcgatc tgggatatct
     5461 ccgttgatcg tgatcagtgt ttgcggagcg atttggtcac gtaaagcaat gattttaggg
     5521 atgacttccc aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa
     5581 agattggcta tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc
     5641 gtatagccga tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc
     5701 aattgtgccg ctacttctgg gcgaaggatc aagccactgc cttttccacg ttctgctaca
     5761 ttagggactg ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca
     5821 atgctcattt ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt
     5881 tcatcttctg taaaggtcaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa
     5941 ctgtctgagt ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca
     6001 tggcgaaata caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc
     6061 agctctttcc aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa
     6121 gtcgcaatct ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg
     6181 aacagcttaa aagatgtttc tgattgagca aaaaaagact gtgacaaaaa tcttatcaca
     6241 attccttttc cggaataaat ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
     6301 aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc
     6361 tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac
     6421 aaacgacaaa atatgaggaa ctattttccc gtttgtttct ctaatactca ggtcaaaacg
     6481 ctgatttcac aacctctttt ctaacttctc aatcctcttc ctttattgat caaataccaa
     6541 gtaacggcgt acaaaacaac gatgaatagc acaagtacga tcatcgatgt tgctactggc
     6601 acatcgattg ttccaaggaa tccgtaacgg aaaccggaga tcatataaac gatcggattg
     6661 actttcgaaa cagcttgcca aaatggcggc aacatagaga tggcatagaa aacgccacct
     6721 agataagtta acggctgcaa gacaaatgta ggcacgatcg atacatcgtc ataagactga
     6781 gcaaaaatcc cgttgagcag tcctgctaac gaaaacaaga ttgccgtcat caataatgta
     6841 atgatgacca tgaaccatga atacacatga agtggtacaa agaacaagga aatgatcgtt
     6901 acgagagagc cgacgagaat actgcgaccc actccgccaa tcacaaagcc ccaaatgatc
     6961 acatgcgtag gaacaggcgc taccaagatt tcttcgatgt ttttctggaa tttttgagag
     7021 aagaaggacg aagaaacgtt ggcatacgaa ctagtgatag cagacatcat aatcaaacca
     7081 ggcacgatga attccatata tgaaaaacca cccatgtcac cgatccgacc accgatcatt
     7141 tttccgaaaa taacaaagta caaggatgtc gtgatcacag gcggaaccag tgtttggacc
     7201 cagatacgca aatagcggtt cgtttccttg atagctaaac tttttaatgc agtaaaataa
     7261 agattaaaca tttttctcct ccgtatgatt attttcttct gtgattttca aaaacagttc
     7321 ttctaaacga ttcgacttat tcctcataga aagaactttg attccttgtc ttgtcagctg
     7381 atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg acagcgattg tctgttcatc
     7441 ttcaaatgta caagcgtatc cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa
     7501 gataaatgtt tcaaattgca gtttggatag aagctctttc atactagtat tctcgatcag
     7561 ttcacctgat tggatgatcc cgatgttgcg acaaagcatt tcagcttctt ctaaataatg
     7621 cgtcgtcaaa atgatcgttg taccattttc gttcaattct cggagaaact cccacatctc
     7681 gcgtctcaat tcaatatcga ctcctgctgt tggttcatcc aagatcagca aacgtggttc
     7741 atgcatcaat gccctagcaa tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc
     7801 acggacattt ctcttttccc aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat
     7861 cgcttctttg cgagagacac cgtagtagcc tgcttgattc acgacgattt gctggaccgt
     7921 ttcaaaggga ttgaaattga actcttgcgg gactaatcca atctgttgct ttgctcggac
     7981 tagatccgta tccagatcat aaccgaagac attcactttt ccagatgttt tattgactaa
     8041 agaagtaatg attccgatcg ttgtagactt cccagctcca tttggtccta atagcgcata
     8101 gaaatctcct tctttcacgg aaagatcgat tcctcgcaat gcttcgaccc cacttccata
     8161 aacctttttc aaattattta tttcaagcgc atttgccatt tatttcctat ctccttatag
     8221 acatgattct ttattgtatc atggaataca gcagatgaaa aaacaacttg gttccagcga
     8281 aaaatcaggt tgctgggctc aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc
     8341 agacaacctt ttcagaaaaa acaaatcgaa gataaaggat tactattcat atgtgcaaca
     8401 aaaggagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
     8461 ggcggaagca aaagccaaga tttttgatcc aataaatact ttcgatcact tcgcctatcc
     8521 caatacatgc cactgccgct tttcatcaaa taacgcggct tccgtttttg cggtttctac
     8581 taaagtctct gggtaatcca aaactttcag caaggcaatc gcatttcggg tccttgaagg
     8641 tccttctttt aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa
     8701 gtggacattc tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac
     8761 aaatgctaaa gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg
     8821 ttcgatcgta tttgtccctt ttaaaatctc atctacgaag cagtagcagc gttctttgga
     8881 agccacttgg tccaacaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc
     8941 ttcaaataaa tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa
     9001 ctgttctgca atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt
     9061 gacatacgta gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt
     9121 gaccggattc actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc
     9181 gccctcagta aactctggtc ggcaagtgat gggcatataa gtacggtaat tcaatattgc
     9241 agcggctact tctaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt
     9301 ttctaattta gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac
     9361 atattcaaaa atcaactctg cttcactccc actcttcata cgaaatgaaa aacctaattt
     9421 agggatgtct cttaatggtt tagtttgctg tttcagctct tcttgaagtg gtgtcggcag
     9481 ttttgctagc tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc
     9541 tgtttctagt gtttgtttct tcactagata atagaccgta ttgaatacta acccaccgat
     9601 tgccaaatag attcctagta cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag
     9661 caagccaaga aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag
     9721 atattgtttt gcaaaattat gatctttttt tcctaaacga gcgaattgat attggatttt
     9781 ttctctcagt tgaggatttt cctgataaaa agcaatcagt ttttctaact gctgatcttc
     9841 accaaaatca aagctgcgta aacgttgata caacgcttct gaacctacac tagaataggt
     9901 actgttgatt ccttcgaata cttcgaacat atccaggtca taccaagtaa gatcatcgat
     9961 ttcgctatcc catgaacgga acttcttctc cgtgagccat gcttccttca agctttcttc
    10021 tttatcaaat ctcggttgat aaggaatttt ttcccatttg ctacgaactg tttcctttaa
    10081 tttcaaacgt gaaaaaatgt ctaaagcaac taataaaata acagctgcaa taataattaa
    10141 aacgataaat tctctttgac ccatatccct ctccgttcct ttatttgtta gctttagtat
    10201 agcactgttg cgctatttga aaagagggta aaaaaacacc tttcaaaata aaaggtgttt
    10261 ttttcttgta tattacatat ttatctctca tttatcaaaa ttgacaaata atcataggga
    10321 ggaattgttg ttaattcttg tttattctcc agtgcattga caactgtccc aagggaaagg
    10381 atagcctcag gtactatttt ttcttgttct gttggattag ctaagcgaag aacaaatcct
    10441 tgttcatgaa aataagaagg ataaatcgat gatacaatta agtcttcagg taattcaatc
    10501 aatgaaaatt tacgaggaac atcaagttca ttttgtaatg gccaaatctt gttatctaac
    10561 ctgtgaataa atacattcag tttctgcttt tgataagaaa tagattgttc taaccgtttt
    10621 tctgcctgtt ttgccacttt gaactcatta aatcttcctt cttctacatt aaatccaaaa
    10681 gtgactttat actcacctat ctcttgagct agcggtgttt ccatcatgac atgcccttca
    10741 tttgtcgtat ctcctgaagc tcgcccaggt cgccaagcaa gattaggttt tccaagttcc
    10801 cctgttgttg aaaataaggt tatgattaat tgattttcta tacgttcata ctcttttaaa
    10861 ccatctgcat aaaacgtaaa ataatgatca ttttctgcca cagagacact cttatcgaat
    10921 atttctaaat taacaggttt ctctaccata gtttctgacc aattcggagg aatgacatca
    10981 ggggtatttt caataaatcc gttttgaatt tgtgcaattg atttttcaga tttatctaaa
    11041 acattaattg ctaagcgcat acgatgcgag taaacagtat tatgaatttt gagtgtgcct
    11101 tcaacgattt gactttctcc cagactgaac gatatttcat aagatatttc ttccatttcg
    11161 ggtttttcag ataaacgatc ttttaaatct ttaggaagtt gagcttttcc gtaaacaact
    11221 aatgtttctt gtaaagaatc tttatatact ttagctgttt ctaattttag agatagttct
    11281 gtatctcctt ctaatggtga ataatcatac gtatcgccat catttgctga atcgatcaaa
    11341 tgaacgaaat caagatattg tctatctcca actatcaaat tcaattgccc atctttaaaa
    11401 gaaagtttat atttattgtt acttatctca gttttgtcta tctcgattgc tgtttctaat
    11461 ttttcattat ttttctcaaa ttcaacaaca gtgtatccta gtgctggcac atttacattt
    11521 atttttatgt caagttcata ataagctggt tcatcaaaat attcgaaacc tgtagcaatc
    11581 atccgataag catttttccg ttctggaaaa taaacttctt tttcaatgat attataggga
    11641 ctacctttga agtgtatttt ctttgaatca gctactacat gcacgatttt cgatccacta
    11701 taattctcag tcgttgtgtt gaaaacaagg acttcattcg attttagctt taatgcatca
    11761 gccatccgtt ttacaataag gttttcgatt ccgtcagcta tctcattagc ttctttgatt
    11821 cgatggaaaa tatcttccgc aacattatct gaaacacagc caccaatact atcatgagcc
    11881 tggttttgca aaatcttttt ccataaccga cgaagtaggc ctttactgat atggatacca
    11941 tttctttctg caatcagcat gagcggctca actcgtttta gaattttttg ttccaattta
    12001 aaattttcta ttttcatttt tgttcttact gagccaatag aacgatgaac acgagagtaa
    12061 accggctgac gaagttcacc tttatattca ggtaattctt ttttgtttct gataacttct
    12121 acataagaag gataatctga aataatattg tggaatttac tgtatttatt caacttctct
    12181 acgacttgat caaaatttaa aatcattgac ttttgatcta cacccgaagg tactaaaata
    12241 tctttgtttt ttccttgcat atgaagcatt tcaactgctt catccaactt ttcttttgca
    12301 aattcaggaa tatctttaat tgcgtctaag gtcatcattc cagtcatata accaaatgga
    12361 aaattcatgg cataaacaaa tttatctccc aatcctttcc agataaaata aggagatgga
    12421 accatcgttt caaagtttaa tcctcgccaa cacatgaaat tatcgatttc tacttgatgc
    12481 aaaagtgtag gcaaattcgc attgaaccca aacgtgtcag gcaaataccc taccatcatt
    12541 ggatctccat atttaaggat cgtatcactg attccaatca ttaagtttct taaaattgat
    12601 tcagcatcca ctaataaggc atctgtttgt gcataccacg gtcccacgaa tagttgtcct
    12661 gttttcatca gtcgttggat aaccttaagc ttttgcggat tgatctccaa atactcatcg
    12721 acgattgaag tttgtccatc taaacaaaag tttactttag ggttcctttc caattcatct
    12781 agaacttcag taaagacctg atcagacaat acgatggcat cttcatttgt aaaataccat
    12841 tctctgtccc agtgcgtgtg ttgaattata tgtgcattta ccatatcaga ttctcctact
    12901 ttccttcttc tactgaattt acttgttgtt cttttttata cttgatggat tttagtgcat
    12961 tgaccattaa cgctgtaacc actcccccaa taacaattga tactaaatat aaaggtatat
    13021 tcgtaacaaa aggaattgcc ataaaggttg aaagcattgt ttcttgagta acaccaaaaa
    13081 atccatttag accacatgct actgcagtgc ccaccacgat gcttggtatt actcgtaaag
    13141 gatctttcac ggcaaacggg atcgtccctt cagtaatccc cacaaggccc ataatcatag
    13201 ctgtctttgc attatctcgt tccgattcat cataaagttt tttgtttaaa attgttgcta
    13261 aaaacattcc aaatggcgga atagcaattc ctaataacat ggctgtacta ggtccaaagc
    13321 ttccgcttga aaaagaagca accccaaaag catatgcgat tttattgata ggtcctccca
    13381 tatcaaacgc catcattcct cctaaaatag ccgacatcaa agcagcattt gctcctgaca
    13441 tgccttctag ccaattagtc agtcctgttg tcattgcttg aataggcgta gctagtacat
    13501 accataatat gattccaaca actaatgtag taacaaaagg aatcagcata attgatttta
    13561 gactaataac ttgcatcggt acttttactt ttttcaatgc attagctaaa tagccacaag
    13621 ctaatccagc aaccattcct ccaataaatc ccataccaag attttgacac atcatcccag
    13681 tgataaaagc tggtgctaga ccgggacggt cagcaattga atatgcgata taggctgaaa
    13741 tcatcggtac gataaactgt aatcccgtct gaccaatcat tcgcaggact ccccagaatg
    13801 tatccgtctg gtcccaaaca cctgtgccac caaaaatgac tgcaatagaa gttagtaacc
    13861 ctgctgctac gacaagcggg atcatatagg aaaccccact ttggaaatga tttagaattt
    13921 ctcttccttg tttcttgaat tccattagta actcccccac gatatatttt ttatttgtta
    13981 attaatgcaa ttgcttcatc aatcgtctct tcaccatctt ttactacctc tgaggtccct
    14041 actgttaaaa tcggtttacc gtcaaagcga cctcgttctg aaacatttgt atctactgca
    14101 aatatgacaa catctgcctc ggaaacatcc gaaattgaaa gtttgttttc tatccccata
    14161 gccccctgtg tttctacttt aatatctaac cctttcttct tcgcaaactt ttctaaatta
    14221 gcttttgcca tataagtatg ggcaatccct gcgatacatg ctgttactgc tactattttt
    14281 gtcatatgaa attcctcctt ttaaaatgat agagctttta ccaattcttt tttatttctc
    14341 gaatctttta ttcttttaac aaactcgtca tccatcaatc ttccagataa ttctgctaaa
    14401 atacgtaaat gattagtacc tttatcagtg tcttttattg ctaacaaaaa gacgagatca
    14461 actggttgat catctaatgc gccccattca acagaccgct ttaacttggc tacagccact
    14521 gttgattcaa ttactgctag gcttttgcca tgaggaatgg cgatattttt cccaattcct
    14581 gtggtcatat ggttttctct gtcaaatacg tctttttgaa attgtttgtg gtcagacaca
    14641 aacccattag tctctaacaa tttcgataat ttttcgacta cacttttttt atcagatgca
    14701 tttaaatcta tcactatatt atttatattg attttttcac tgatcattta taagccttct
    14761 ttcaatatat aaactaattt ttcaaacgac gaactcgttt ttaaatttgc taataactca
    14821 tgattcgaag cgatttcatt gaatgtttca taaattttat tcaattcagg ttttactttt
    14881 tcattaagtg caaaaaagaa aacagaatga acaagtgtcc catcccaatc aattccttct
    14941 ggattaacgt agacacagat tactggcttt tttataaaat ctacttcgat atgcggcata
    15001 gcaaaatcat tcatcgctgt tgaagatagt ttttctcttt tgattgctcc ctctagtatc
    15061 ccttctttcg cgtaattttc ttttaccaat tttttggtaa ttatatttaa aacgttcact
    15121 tgattatcct ttatttggtg ggtaaaaaag caaaatcgtt tatctaataa atttagaaaa
    15181 gcatcttctg tttgttcatt taactgtaga tactgtttta cttttctgac atctgcccct
    15241 aacatcaaag gactaacaac aataacagga aaatttgtat tttcaatcgg aatcgtgctt
    15301 ataacaagtt catcagtgac ttttctatct tgtagttcat tgatgcttag tacatcttct
    15361 atctcaacca tatctgcaaa accgttattt attctttgct ctaataattt tgatgttcca
    15421 tatccgctac tacaaactaa tatcactttc gttttgtcag gtttgtaacg atccaaaagt
    15481 gattgaatat ggagtgttac gtaagcgatt tcatcttcat taaaatgtat acagaatctc
    15541 tcttcgattt cctctaataa atctacactt atcataaatg accttttaaa agaatatttg
    15601 attttgtccg tataagggtt atagatattt actcccaatt tgagtctttt aactgctgca
    15661 ttgagatgta atactagatt attgatcaat tcttcatcag gataaaattc atttaattta
    15721 attcttacta actcttgtag ctcaacaccc atatctaaat aggaaagctt cgtttgatct
    15781 aagtcgtgat tcatagcatt tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt
    15841 tcatctattg gcacaataat tgaaaatgct ttttcaatca ttttgattaa aataagtgta
    15901 ttctttgaga tttcttcttc agaattatta ctacgtgata agaaattgct tgtttcaatc
    15961 ctttctatcg caatagcaag gtggatagct aaagtttgaa attcataatc tgtaaatttc
    16021 aattgtgtct tattcatgta tcgattaagc aattcggcta cttttttcaa tatgtcctta
    16081 tttaagaaat tattcgcttc accaatgaaa ttgatattca agtctaagag atctgtttta
    16141 ctgttagatg ctgtaaagcc accccaataa tagttcacaa cttctgacat caaccttctt
    16201 ttactttttt catcagcttt gatgcgcacg ccatttcgat ctgaaactaa ggacatccca
    16261 tgcttgttta gtctttttct aacatctttc attgtgcttt ctaccgtcga cctgctcacg
    16321 tataattcgt cagcaagatt ttgaatcttt agagaatcat tagtattcaa taatttagca
    16381 aacacataag ttagacggtc ctccggagag tctgcgatat tgattttatg actggataat
    16441 aaatttcgaa gtgtttcttt ttttccaata agcgaaacgc cttttttagg ttgaaccaac
    16501 atttgtacat ctggcatttt ttggattatt tctttcaaaa ttaaaatatc tttgctaatt
    16561 gttttcgtag atacatataa ttgttctgat aattctttgt aagttgtgtg ctcattttca
    16621 atcaaaaagt atagtatttt catttgccgt tcgctaaatt cgatgcccat tatcttcacc
    16681 tcctgaatac agcttaagtg tacaattatt atctctattt tcatacagaa gaaagttctt
    16741 gattaatgta gaagtttcta atattcttga ttatttcaaa tcaataaaaa agcctgcaat
    16801 atttatatca caggcttttt tctctattaa atatatataa gataatacat ttttctacta
    16861 gttctcacta atcttgtata attaaaccct ccgcaacttg acggagatgg agaagatgcc
    16921 cccgtctaat ctagcggtca tctctcctcc cattccttcc accaattttt ttgacaggta
    16981 aagcccgatt ccgctagatt ccgtatttgt gcggctggta ttttcggtat aaaaacgctg
    17041 gaagactttt tcaacagagg tttgcgttcc tggtttgata ggattggcca ctgttaatcg
    17101 aatgaatgga tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa
    17161 attctgtatg atattttgca gcactcgcag caacatgagc ggatctgcta atacagttgt
    17221 atttatcgag tgttcagcga acgctacctc gatatttttc tcagaaaatt gttcataaaa
    17281 agagaaaaat acttcttcca cctctttaga aaaagaaagc gtatggagat ccatttcttt
    17341 ttgcttcgtc tctattcgag caagatcata aaactgatcc gtcatactga gtaatcttgc
    17401 aacagattgt ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct ctacctgttc
    17461 cagtaattgt acataaccgt taatagcagt caaaggagtt ctgagatcat gagaaaggcc
    17521 gacgatcgat agtctgatgt tttcttccat ttcttgaaat gcttgtcctt tttgttcatt
    17581 ttcatccaac catgtattca gtgcatctac taggtccatt agattctttt ctcgaaattc
    17641 gactgaaaga cgaccaccat attcttgtcg ttcagaaaga gtggcgattt tcttagttaa
    17701 tctcttgatt tccctttgca ggaagaaaat aaaacttccc ataaagataa caacgagaag
    17761 caacagccat ttaatcaaat ctaaacccca ctccccacac cgtatgaata tacacagcat
    17821 gtgcctttgc tagcttccct cgaagattgg aaatatgcat attcactgtt ttctcactat
    17881 caaaatatgg ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat acttttttcg
    17941 gatgttctaa aaaaagcttc agcagatcga attctcttcc tgtcaaatgg acagccacac
    18001 cattcacatt tacatccctc gactctttgt ccagtaagat ttccttgtaa tagatgcgtt
    18061 gcgacagatc ttttacttgg acagaagtct gatgacgaag ttggatgccg attcgtaaca
    18121 aaagttcttc gatatcaaat ggtttcgtca catagtcatc cgctcctgct gccaacaagg
    18181 aaactttatc tctttgatcg ctttttgctg aaagaacgat gatcggaaca ttcgatgtct
    18241 gtcggatttc tgctaacaat gcttcacctg aaagccctgg aagcatgaga tctagaagga
    18301 tcaaatcgac aggatatgtg cttaacaata attttccttc cgtacctgaa taagcatcaa
    18361 gaagtttgta tctctgttca agaacctcct ccagtaagga atgaacagct tcatcgtctt
    18421 cgataatcaa aatagtttcc agcaggacca cctacttaat gtcagatttt tggaaaataa
    18481 acattcctag agctgatgat accacgattg ttactagact gaccagaatc attttcggtg
    18541 cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc
    18601 actccgttag aaattccaag cctttgattt ttgcgaaaac accgggaatg aaggccgttc
    18661 ccatgatata aagaaaagaa agaagcaaac tgacactggt acttctagtg atgaatgtca
    18721 aagccactgg aacagataat gtccctaatg tcagcagaag caatacagga aatactttta
    18781 gaacataatt tccatagttt ttgattccct caacttcacc aaatccgaaa gcaagtgtgt
    18841 aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa
    18901 cgagaagttt cgacacataa taagtcattc gattccgtcc taaagataac gtattgcgaa
    18961 tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc aaatgctgcg aaagccagaa
    19021 gaatccatgc agccattttt tctaccatta aaatccctgt tgcaccgttg atgactgtat
    19081 ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat ataccattcg ttccaagaat
    19141 aaaaaagcgg aagtacgacc atcagtccta gtgagaccca cattttacgg ctcttccata
    19201 cttttatttt ttcagcagtt aatagattaa acatgtggat tgcctccagt taattgtaag
    19261 aaataatctt ccagtttttg cccaactagg tgaataccat ccacaggaac ttttgcttct
    19321 accaatgctt gattgatggc tgctacttgg tcaattcctt caaaaagctg gataacacgg
    19381 ctagattgta caaaataatc cctataacca agagagtcga gaaccgttac tgcttgttcc
    19441 gtcgccgaag tgtcaatttt tataaattga cgagattctt gtgccaattc ttctttcgat
    19501 aattctttga tcaagtgtcc ttcatgcaaa ataccatagt gagtggcgat ctgtgacaat
    19561 tcatctagca agtggctaga aagcaagacg gtgatgcccc gttcttttac caaacgttgg
    19621 atgatttctc gcatttctgc catccctgct ggatccatcc cattcgtcgg ttcatccaaa
    19681 atcaaaaatt ctggattcgt gatcagtgca tttgcaatag ctaaacgctg gcgcatacct
    19741 aaagagaaat tctttgcttt ctttttccct gtattttcca atcgcatcat tttcagcagg
    19801 tcttcgattt ctctatccga cacaccgccg ttcgctgctt ggatacgtag gttatctctt
    19861 gcagttaatt ctggataaag agcaggggtt tcaatcgatt gacccatacg tcgtcttgct
    19921 gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt catcaatcga gatcaaaccc
    19981 ataatagttc tcataaaggt agacttaccc gctccatttt ccccaatcaa tccatagatc
    20041 attccacgtt tgatttcgat tgatacttta tctaatgctt tggcagcacc gtattttttg
    20101 gttataccag ttgctttcaa gaccgtttca tttccaaaat ccatacttat ccttctttct
    20161 tattgatgag tgaagtatag ttgacgggta taaagaaagc agccagaaat gtataaagaa
    20221 agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg acaatacgtg tctttcctat
    20281 tatcaggcag tttgttatac gcttgtatca gtttcctgat tctttctttt cgaccaaagt
    20341 cgtttctctg tattcctctt cactgaaaat ttttgcttct ttcagatcaa gcttgagtaa
    20401 ttttaccaga ccggttataa ggataactat agcaagactc cctcccagaa aaattagact
    20461 gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc gcaccaaaca gtgctaatcc
    20521 aagtggactg gccaccatgg taatcgttgt tatcgttgac ataagtcgtc cttgtatatg
    20581 cgttggaact tctcgctgaa gccagaccat ataaaaagca aaagaaaaca ttaatcctta
    20641 aaaacggact tatgaaaaat aatacatgcc gtttgggatt tacaattagc taccaaataa
    20701 gaggttatga caaaagactt ttcacaatcc cttcttccga ataaacggtg ttctcctgcc
    20761 aactcctcgg tcacaagtca caatatttat caaacatgag aagcaatttt tgtaaattcc
    20821 ttcttatttc ttgaagctga ccacttctgt cacaacctca gaagcggata aataatattg
    20881 tttgatgata ctttatcttt ttcccgtttg tttatagatc gaatgatatt atagaccaag
    20941 aatcctacct attaacatag ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt
    21001 gccagaaaag ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc
    21061 gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg aagctttacg
    21121 ggctttctta agacctggtt ttttcttcgt atcattgaat aagtttgaaa aaccttgatt
    21181 tataaaggtt ttcccgtaaa tatattttat tata
//
LOCUS       DACCWK010000041        20948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCWK010000041 DACCWK010000000
VERSION     DACCWK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.041"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(92..1465)
                     /locus_tag="IVW65_001458"
     CDS             complement(92..1465)
                     /locus_tag="IVW65_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7900341.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1465..1860)
                     /gene="rbsD"
                     /locus_tag="IVW65_001459"
     CDS             complement(1465..1860)
                     /gene="rbsD"
                     /locus_tag="IVW65_001459"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7900342.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1888..2805)
                     /gene="rbsK"
                     /locus_tag="IVW65_001460"
     CDS             complement(1888..2805)
                     /gene="rbsK"
                     /locus_tag="IVW65_001460"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7900343.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2913..3926)
                     /locus_tag="IVW65_001461"
     CDS             complement(2913..3926)
                     /locus_tag="IVW65_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7900344.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(4007..4606)
                     /locus_tag="IVW65_001462"
     CDS             complement(4007..4606)
                     /locus_tag="IVW65_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7900345.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4792..5493
                     /locus_tag="IVW65_001463"
     CDS             4792..5493
                     /locus_tag="IVW65_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900346.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5503..8214
                     /locus_tag="IVW65_001464"
     CDS             5503..8214
                     /locus_tag="IVW65_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7900347.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8665..10293
                     /gene="treC"
                     /locus_tag="IVW65_001465"
     CDS             8665..10293
                     /gene="treC"
                     /locus_tag="IVW65_001465"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7900348.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10353..11534)
                     /locus_tag="IVW65_001466"
     CDS             complement(10353..11534)
                     /locus_tag="IVW65_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7900349.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            11786..13309
                     /locus_tag="IVW65_001467"
     CDS             11786..13309
                     /locus_tag="IVW65_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7900350.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(13378..14106)
                     /locus_tag="IVW65_001468"
     CDS             complement(13378..14106)
                     /locus_tag="IVW65_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900351.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIIEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(14119..15399)
                     /locus_tag="IVW65_001469"
     CDS             complement(14119..15399)
                     /locus_tag="IVW65_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7900352.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15540..16142)
                     /locus_tag="IVW65_001470"
     CDS             complement(15540..16142)
                     /locus_tag="IVW65_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7900353.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            16387..16593
                     /locus_tag="IVW65_001471"
     CDS             16387..16593
                     /locus_tag="IVW65_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900354.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            16905..17624
                     /locus_tag="IVW65_001472"
     CDS             16905..17624
                     /locus_tag="IVW65_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7900355.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(17705..18388)
                     /locus_tag="IVW65_001473"
     CDS             complement(17705..18388)
                     /locus_tag="IVW65_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7900356.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            18606..20792
                     /locus_tag="IVW65_001474"
     CDS             18606..20792
                     /locus_tag="IVW65_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7900357.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7163 a         3749 c         4077 g         5959 t
ORIGIN      
        1 agtatcacaa aaatttataa aaataatgat cctaaaaaga aaaagactga aatatgtgat
       61 aaacatctca tacttcagtc tttttcatta actattcata agacattagg tcttcatcaa
      121 acccgctaac gagagtagtg atcattgcta cgactagact aacagccagt cctgctaagt
      181 accagaaaaa agtaccggaa atgaatgcag gcaatgtggt cagactacca aatacaaaag
      241 cgatcgcttt cacttgggca aatcccatta cagctccacc agcagctgca ccaatgcaag
      301 cagcaacaaa tggttttttc aatgggatcg caattccgta aacagttggt tcggtcactc
      361 ccgcaagtaa aatcgaagca aaagcagaac cggagatcga acgggttttt gaacgtttac
      421 ttcgcagaaa tacacctaaa gctgcaccag ccattccaaa attggctgct cctgtcaacg
      481 gaacgattcg gtcgaaccca taagcagaga tattactgat catgacaggg tttacagccc
      541 attggatacc aaatattacc aaaatattcc aaccaccagc gatgattgcg ccactgatcg
      601 gtccgttaat atccatcatc caagtaattg cggcagctaa accttcgcca aggtaaacac
      661 ccgcaggccc gaataccatt aacgtcaaag gaaccgtgat aactaatgaa agtaaaggta
      721 caaatactaa ttgcatattt tcgcgaatat attttttgag gaatttttca acaaaagaat
      781 aaaagaaaat acctaaaatc gccggcatga cagtagacgt atactgcatc agtactacag
      841 gaatccccag aaaatcagta gctgctttgc cactgtctga aagtaaagag gtcagattcg
      901 gttcaatcag tgctgcagca atgacagccg caatatatcc attcacttta aacgttttgg
      961 cagcagaaaa agcaagaaag attggcagaa aataaaagac ggcattcgac gcagcagaca
     1021 aaatatgata agttccgctt gtttcactaa gccagccaat cgtagtcgct aaaacaacca
     1081 gaccttttaa tataccagaa ccggcaaaca gcggaatgat cggcgtaaag atttccgata
     1141 tcaaaccaaa tagtttttcg atcggattta gcttttcttt cggagctgaa tgatcgattt
     1201 gacgcgtcaa ttccttgtaa gcaggagcca atccctgatc catgattatt tctgcatatc
     1261 tatcgagtaa aatgaaatac ttaatggaag ggatttgctg ttttaatttt tctgcattgg
     1321 ctactggctg ttttttcaat ttgatacgaa gccgagtcgc acatggaagg acagaatcaa
     1381 tattgtcttt tcctcccaac tgttcaatca gcatatgaat ctgctcggac agtaccatct
     1441 gttcaggatt cattgtcgtt tccattagaa gaccactcca ctcactaata taatattggc
     1501 gtaaggtgtc atttcgcctg tacgaataaa tgcatgacag ttattcaact cttttttcat
     1561 ttcttcatgt gggataaagc taatcggtgt atctggtaac aataatttga ttttttccaa
     1621 tatttccggg ttcattgtct ttatttcttc cgccaattca atcgattgta cctcaagttc
     1681 ttctaaaacg ttttctaaaa cagataaaaa acttggaaga ccgtttgaga cagccaaatc
     1741 tattttttca gtaccaaagg ggacaggcat gcctgcatcc ccgatactta atttatcaaa
     1801 gtgccccatt tgtgcgatta ctcgtgaaat atctgaattg attacttttc cttttttcat
     1861 gtttgtttct tcctctcatt gttaatttta gactgctgat tgttctaaag cttcgcggta
     1921 agggatagaa ggttgcgcac cataagactg cacagcaaca gaagaggcta gatttccata
     1981 aaggatcgct tctttcaagt tactaaggtc tggctgaagt gtgctgctta atgcgccaat
     2041 aaatgtatca cctgcggctg ttgtgtcaac ggcatccact ttaaaagcag ggacgatacc
     2101 gtgttctttt tctgtatgat agaaagcgcc ggcactgcct agtgtaatga ttactgttcc
     2161 aatacctaat tcatggagtt tttcggctgc tgcgaccaaa ctgttatgat ctgtcactct
     2221 gattcctgta atgatttctg tttctgtttc atttggtata atgatatctg tcttttcaag
     2281 tagttcagta ggaatcgttt cacgagctgg tgcaggattc aggatcgttg tttttcctgc
     2341 gtcttttgca atagaaaaag ctaggatcgt cgcatctaag ctactttcaa actgagcaat
     2401 aataaagtca ctgtttgtaa tcagttgctt gttttttagg acttcttgtt catggaaagc
     2461 attatttgcc cctgcatgga tcaagatact attttctcct gctgcgtcta ccataatcat
     2521 tgcttgtccg gttgtggcgc cttggatttc agctatccca gatgtatcga tgttttcttt
     2581 tgtcagtaga tctaataact gttgtccttc gctgtcagcc ccaacgcctc caataaaaga
     2641 tgtccgtgcg cctgaacgtt tggctgccac tgcttggttg gcacctttac ctccgccagc
     2701 gtgaaaaatc tcatgcgcat gcatcgtttc gcctggcttg ggcatgttgg ttaaacgaag
     2761 ggtagtgtcc atattaatac ttccaattac tgtaaccgtg ttcattttac aaacctccaa
     2821 ctatctcatt tattttgatg ggaaaattat tttttaaaac gttttagtaa aacgttttaa
     2881 tagttcgata aaaatatagc acaccttgaa agctatttca agggtgcagt tgaaaatctt
     2941 ttttctaatc gtacaggtaa tcttttttct tcccattctt tttctgggaa ttggatccga
     3001 tctaacaata acttggcaga tgtttgtccc aattcgaaga ttggttgtgc gatcgttgtc
     3061 agtttcggct tgatatactc gcacatatct atattatcat agccaataat gctgtagtct
     3121 tcagggatgc tttttccagc ttcctctaat ccgcgatata atccaaaagc taattcatca
     3181 tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt ctgctgtgat ctggtagcca
     3241 ccttgttttg agaactgtgt aggtaagagg atcagttggt cgtgagggat ttggtaaaca
     3301 tcaagagcag atttaaaccc ctcaatccga gcgtggacat tttccggtgg attttcagga
     3361 tagacaaggg cgatcgtttg atgtcctaga ctaagcaagt gcatgcccgc taggtaacca
     3421 ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt cagatttttt ctgatccaaa
     3481 acaatgaagg gccggccttg ttttttcaaa ttttcgttga ttgcatctgt agagacagca
     3541 gaagtcgcga taatgaatcc atccacacct cgacgtgtga gctcggctag atattcgatt
     3601 tctttttgat gatcagaatc agcattacat aatatcgtga cgaaattctg cttatacaag
     3661 atatcctcaa ttcctcgcat cagtgtactg aagaatggat tcgtgatatc aggaacgagt
     3721 acaccaattg ttttcgtttc cttggtaatc atttgtctag caaaatagtc aggctgatag
     3781 cctaattctt ctttggcagc taaaactttc tgaacagttt tcggactgaa tttctccgta
     3841 tttccattta atatcaagga gacagttgtg attgaaacgc cggaaagttt agcgacttct
     3901 ttgattgtga tctttttttt caccatgttc atactcctat ctaaatcctg tggttatttt
     3961 cccatgaaat caaaaagaag taaatgaaaa tcacatgtgc cagtacttat ttgctttgtt
     4021 ggatagcttc gattatttgt ttcgttgagc gtactcgtgc agttttcgga aatatctttt
     4081 ttatgaacaa ttgatgggat tctttgtcac gatcactgca agcatcttca gcaagcacga
     4141 tatggtaccc gtgttgaaag gcatctaatg cagtagcata tacaccgttt gttgtcgcta
     4201 caccgcttaa aatgatagtt tcgattccac gccggcgaag ttgaagatca agagaagtac
     4261 caaaaaaagc acttggattg tattttgtga tagtcaacat attatctgta tctttcaaag
     4321 aatccttcaa aagtaaatca gtgaattctg gaggtacaga agcagtcatt ccacccatat
     4381 cagtttgttg actcaaataa gggaaagtgg agatgtctac gttcactaaa actttcaagg
     4441 cagcggtacg tttcaatgtt cttactaaca aatcattgtt tgtcagaata gatgctgcag
     4501 tgtgtggaga gagtgtaccg ttatttacga tctctttttg catgtcgatc gcgacaaaag
     4561 cagtttttga taaatcaaaa atcatatttt cctttaaatc attcatgatg ttccttcttt
     4621 caaataacaa tgttttttat gtaagtaaat cataaatcgt ttatttgtca actaaattaa
     4681 aggattcctt tctttagtct agagaaattc agtggtaaaa taaataagtg tgagaaaacg
     4741 cattcgatgc atagatatag cgagaaatat cgtgaaaatg gaggaatcag agtgaagtat
     4801 gtcgaagtaa aaaatgaata caaacgatac aaaatgggag aaacgaccat tacagcaaat
     4861 gacgatattt ctttttcaat tgaaaaaggc gaattagtga ttatacttgg tccaagtgga
     4921 gcaggaaagt caacgattct taatatttta ggtgggatgg atacacctga tgaaggtcag
     4981 atcattattg atgacacaga tattgcgcaa ttttcagata aacagcttac tgcttaccga
     5041 cgaacagatg tagggttcgt tttccaattc tacaatctcg ttcctaatct aacagcaaaa
     5101 gaaaatgtgg aattggcaac ggaagtttct cctgatgcgc ttgatcctgt agaggtattg
     5161 aggcaagtgg gcctagctca tcgattgaat aactttccgt cccaattatc aggaggcgaa
     5221 cagcagaggg tttcaattgc tcgtgcacta gctaaaaatc ccaaacttct tttatgtgat
     5281 gaaccgacag gcgcgctgga ttttgaaacg ggcaagcaag tattgaaatt attgcaaaac
     5341 gctagcagac aacacggcaa taccgttttg atcattacac ataattctgc tttagcgccc
     5401 atcgctgata gagtcattca cattaatgat gctaaagtaa ggtctgttga attaaatgat
     5461 catccttcat ccattgatga gattgtatgg tagggggatt ttatgaaaac aagaacatat
     5521 cttaaagcag gttttcgcga aattcggcaa tctaaaggac gattcattgc aatcatcctt
     5581 attgtcatgc taggaacact tttatttgta ggtgtcaaaa ctgctggacc tgtcatgcaa
     5641 aaaacgatgg atcactatgt tggtacagca ggactttctg atttgcaaat cgtttctact
     5701 ggtggcttga cacaaaaaga tattgctcaa gcagaaaaga tcccagatgc aaaagtagaa
     5761 acaggaaaac agatctatta ttccaatact ggcaaaaacg aagtgattca aatattttct
     5821 tataacaaaa ataacaagca gaacaagcta caattaactg atggaaaact cccggaaaaa
     5881 ccaaaccagc ttgtattgga tgaaaaagcg gaagatgaag ggtataaaat cggtgatatt
     5941 tatcagattg attcagatga attgaaagaa aaagagtata ccatcacggg ctttgtccgt
     6001 tctccattgt tcatcaacaa tttggagaga ggatacgcga acgtcggaaa tggtagcgtc
     6061 gattattttg tctatttacc tgaatccgtt tttaaaacag atatttattc tgtcttatat
     6121 cttgatttta caaacgtaaa gaatttgaat acgtacagca aagcttacaa agataaaatg
     6181 gagcaaaatc aagaaaaggc agaaaagtat ttacaagatc gacccgaaga acgcttagaa
     6241 gagctgaaaa aatcggccaa cgaagaatta gaaccggcac agcaaaaaat agcggatggc
     6301 aaagcacaaa caacgcaagc aagaacacgg ttagaagatg ctaaaaaaca gttagcgcaa
     6361 cagtcagcaa ctatccagca gcttcctgaa gcgcaacgaa caatcgctgt tcaaacatta
     6421 tctcaacaag aagctcaact tgctgaacaa gaaaggcaat tagttgagaa agaacgtgaa
     6481 ctggcctctg cagaggaaga attgaatcga aatgcagaaa aaattgaaaa tttaactaaa
     6541 ccatcatacc tatacaatga acgttcagaa aatgtcggtt ttcaagaatt tggtgatctg
     6601 tctgatcgta tcgctgcgat tgccaatgtt tttcctgttt tctttttctt tattgccgca
     6661 ttgatcacct ttacgacaat gacacgtatg gtagaagaaa accgcaggga aatcgggaca
     6721 ttaaaggcgt taggttatac gaaagttgaa atcgcaaaaa aatatgcaat ctatgcaagt
     6781 ctagcctcag ggataggtat tgttttagga acgatattag gaacgaatct tttgccaagg
     6841 atcatttttg agctgtctaa cgagcggtat gatattggga gtgcgcttat tttttatgac
     6901 tggccgccga ttttacaagc agctgccgca ttcttcattg ccgcatttgg cgctgcaatg
     6961 atcgttttat tcaaagattt aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg
     7021 aagccaggga aacgcatttt tcttgaatac atcactccgc tgtggtcacg ccttagcttt
     7081 aatcaaaaaa tcagttaccg aaatctcttc cgatataagt caagaatgct tatggcgatt
     7141 atcggaattg ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa agattctcta
     7201 agttctgttt cagccaaaca atttggaccg atcattgact atcaagcaat cgttacattg
     7261 gattcatcca cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa
     7321 gtaaatgaga tcctagctat tcatactcaa accgtagaat tgagaaaaaa aggacatgct
     7381 tcgcaaagtc tgacagtgat cgtaccaagt aaaacagctg atttggatcg ttttatccat
     7441 ttaactgatc ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa
     7501 aaagtagcag aactatttga cttatctaca ggagatcata tttcgcttta tgatgaagat
     7561 caaaaagaat ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg tagctttatt
     7621 tatatgtccc ctgaatatta tgcttcagtt actgatcaat cgatgatgga taatgcattt
     7681 ttgctgcgta cagataagat gacaacaaaa gaagaagaaa tgctttccga gaaactgctt
     7741 gccacagacg ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa
     7801 tcaatggcga acttagactc ggttgtctta atctttgtcg tattatcagg actgctggct
     7861 tttatcgtct tgtataatct aactaatatc aatatttccg agcgtgtacg cgaattatct
     7921 acaataaaag tccttggatt ttttgataaa gaagtcacga tgtacattgt tcgagaaaat
     7981 atcatcttta ctcttttagg aatcatcgga ggatttggtg taggatatat attgactgac
     8041 ttcatcttgc aacaagcgtc tatggagaat gtcatctttc cattagtgat cacatggtgg
     8101 gcttatgtct tatcagcagg attaactatc gtatttacgg ttattgtgat gattgttacc
     8161 catttcaaat taaagcatat cgatatgatt gatgcgttga aatcgaatga ataaaaacag
     8221 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggcaa aatagttttt
     8281 catattttgc cgttttttga gctaataccg aaggacagct gattgaacac catgaatcag
     8341 aggttgtaaa aaagccgtcc tgcatcgagc aaaaagaata ctcaaataaa attagctcct
     8401 catgttttac atttgagaag actgattgcc gtagataaaa gcttttgaac accatttata
     8461 gaagaaacaa gaatattttg ctattttaaa caagaggata gacaaataca tgtctgatct
     8521 cttgtttttt attgggcaac agacctctta gaatgtataa agataaattt tacaaacgct
     8581 ttcggttcag ttataataat aaatgataac ttgtatatat aagttgtggt aggtaataaa
     8641 tcagttgaat ggaggtaaat atccatgaca tttaaagaca aagtcattta ccaaatctat
     8701 cccaaatctt acaaagacag taatggtgat ggaattggag atttaaaagg aatcaaagaa
     8761 aaactcccgt atctgaatga tctaggcatc gacatgattt ggctaaaccc tatttatcca
     8821 agccctcaaa aagacaacgg ttatgatatc tctgattaca cagccatcga tcctatcttt
     8881 ggtacgatgg aggaattcga acagctaata gccgaagcaa agaaataccg gatagaaatc
     8941 atgctcgata tggtgctgaa tcacgtttcg acagaacatg aatggttcaa aaaagcacta
     9001 gcaggggacc agcattatca ggacttcttc attttaagag aagagccaac ggattgggta
     9061 tcaaaatttg gtggaagtgc ttggtcgcca tttggagata cggggaaata ttatttgcac
     9121 ttatatgatc ggacacaagc tgacttgaac tggcggaacc aagcagtaag agaggaacta
     9181 ttcaaagttg ttcgtttttg gatggaaaaa ggggtaaaag gattccgttt cgatgttatc
     9241 aatgttatcg gtaaagacga acaattgaaa aataataaag aaaatgatgg caaacctgaa
     9301 tatacggaca aaccaatcac ccatacgtat ctaaaagaat tgaaccaagc aacgtttggg
     9361 acggattcca ctatcatcac agtcggtgaa atgagctcta cgacgatcga aaattgtatc
     9421 ttgtatacag aacctagtag agaagaatta tcaatggtct ttaatttcca tcatttgaaa
     9481 gtcgattatg aaaatgggaa taaatggtct actcctccat ttgattttgc agaattaaag
     9541 cgactatttc atacatgggg agaaaagatg agtgagggta acggctggaa tgctctattt
     9601 tttaataatc atgatcagcc acgtgcgctc aatcgatttg tagctatcga tcagtatcga
     9661 aaacaaggag cagagatgct agcaaccatg atccatttga accgtgggac gccctatatt
     9721 tatatgggag aagaaatcgg catgatcgac ccagactatt catcgatttc cgactatatt
     9781 gacgtagaaa gtttgaatgc ttataagtta ctccttgaac aaggattcac agaaaaagaa
     9841 gcgtttcgtc ggatcaaagc aaaatctcgc gataactctc gaacacccat gcaatggtca
     9901 gacacagaac aagcaggatt tactacaggg aaaccttggt taaaagttgg aggcaagcta
     9961 gaagagatta atgtagaaaa agaactatct tctgaagatt ctattttgtc ctattacaag
    10021 aaattgattc ggctgagaaa aacatatcca atcgttgcac aaggagatta tcatgcttat
    10081 gaagcaaatc atccggaagt ttatggcttt cttcgccaat ttgaaggaca gcagctgctt
    10141 gtattaacaa atttctatga taaagataca aggatcgcca tcccagaagg attcacagaa
    10201 gccaatattc ttatcgataa ttattccgat actgttcttc atcaaagtct ggagctaaaa
    10261 ccataccagg caattgcttt gctgaaaaat taaaacaaaa acacaaaacg tcgttcctac
    10321 gagctattct cgtaagatcg acgttttgtg ttttatttaa aataatcttc ttcagaaaat
    10381 gaagagtttt catactctcc tttatcgcca taaaatttat tgtagcgttg tttgaattta
    10441 cggaaaataa aagtaacgag taatttgact aacgtataga taggaatacc aaaaataacg
    10501 cccacaattc ccattagatc acccatgaca agtaaaacga ttaagatcgt aatcggatgg
    10561 atctgtaaac gatcacccat tactcgagga atcaccaagt caccgtggag aagttgtacg
    10621 gcaaaccaga caatgacaaa tttgacagcc ataaaagtag agtcttggaa tgcaatgatc
    10681 aaacctggaa agaaggcgat aaacggccca atatatggaa tgatacataa caacccagca
    10741 gaaatagcta aaacactagc aaattctaat ccgatcagta agaaacaaac ccagtagact
    10801 gctccaagga taaatgaagc aatgatttgc ccctttagaa atgaacccaa ttgttgatta
    10861 gcaatctttg tcaaattttt aaaatctgta cgaaatgctg gtgggacaat cgctaaaact
    10921 gactgataaa atttttgagg atctctcaaa aggaaaaaag cgacaattgg accagtaaat
    10981 aaagtaataa aaatagctgt aacagttgaa aaaatgttgc tgatccgttg tgcgccactt
    11041 tcccaataat ttccaataaa tccagctata tcagacgtca cgtcgtcaat agaagcgaaa
    11101 aattgattaa atgaatcggc aaaaggtgtt ttctccaaaa attgtcggag actctcttga
    11161 aaatcttgaa acaaatctgg caaactactg atcaatgtct ttgcctgttc ttcaagaaaa
    11221 ggaataagcc atatgcttcc aagccccaat aaaaataaaa taatcacata aacgataatg
    11281 actgacaagt tcctcgaaag gtgcttgctc aaacgtttga tgactggttc caaaagataa
    11341 tacaagatca gtgcaaacaa taatgggggc aagatagtag cgataatgat tccgacaggc
    11401 ttgaatataa acgagatctg atggaaaaaa taaatgatca gcgcaagtaa gataactatt
    11461 cctaaaacat agtaagaggt ttttcctcct aaaaagcgga tcaaacgatt cgttgtatct
    11521 gtttcttttt tcactctttt tcctccttta tctataaatt cattatagac aaaagcgggt
    11581 ataaaaggaa aatgatagag gttgattgca aaaatatcgt caatcattca tataaacttt
    11641 ctcatcaatt aaaataagaa aacgcttgac agttctttta accatatgat aaattaaatc
    11701 ctgttgctaa aacgtaacta ttacggtttg cgaaaagcga atcggaacat tcaaatgaaa
    11761 gaagatgaga agaggagtgt cataaatgaa acgattgata ggtatattgg ccatgctggt
    11821 tttagcaggt atgttaactg cttgcggggc aagtgggaaa gccgaggaca gtaaagaaaa
    11881 attgagcgtg atgacgacct tttatcccat gtatgatttt acaaaagcaa tcgtcgggga
    11941 tgaaggagaa gtagagttat tgattccagc agggactgat tcccacgatt atgaaccttc
    12001 tgctaaagac atggcaaaga tccaagatac agatatcttt gtttataatg atgaaaacat
    12061 ggaaacatgg gtccccgcaa tccaaaaaac attacaagaa ggcaatgtcc atacgataaa
    12121 agcgacagaa ggtatgcttt tacttccggg aagtgaagaa ggacatgatc atgaccatga
    12181 acacggggaa gaaggccata cacatgagct agacccacat gtatggctag ctccaagttt
    12241 agcaatcaag caggtagcaa acatacgtga tcagctgatt gaagcttacc cagaaaaaca
    12301 ggaagtatgg acaaaaaatg cagcagccta taccgagaaa cttcaagcac tccatcagct
    12361 ttatcaagaa acctttaagc aagcaaaaca aagatctttt gtcacgcaac atgctgcatt
    12421 caactatcta gcgttagaat atggattgaa tcaagtatcg attgctggtc tttcttctag
    12481 tgaagaaccg tctgcggcga gaattgctga actgaaacac tttgttaaag aacatggaat
    12541 caactatatt tattttgaag aaaacgccaa agatagtatt gccagaacgt tagcaaacga
    12601 agcaggtgta tctttagaag tattgaatcc tttggaagga ttgacgaatg aacaaataga
    12661 aaatggagaa aactatcttt ctatcatgga agcaaacctc gaagcgttga aaaaaacgac
    12721 agagacagaa aacccgctag aagatacttt gacccccaaa aaagaaaaga gcgtctacaa
    12781 cggttatttt gaagacgagg atgtacaaga tcgctctctt tcagattggg caggaacttg
    12841 gcaaacagta gatacgtatg tagaagatgg gacttttgat cctgtttttg aatataaaga
    12901 aaaattgaat caggataaga cagcacaaga atataaagaa tattatacga agggctatca
    12961 aacagatatt gcttcaatca agatcagtga gaaagagatg gcatttacgt ttaagaatgg
    13021 aacaacgaaa cgttcaaatt atcgttatgt agggaaagaa atcttgacct acgaggcagg
    13081 aaaccgtggc gtaaggtatc tattcgaagc agaagataag gaaagtggcg cttttagata
    13141 tattcaattc agtgatcaca cgattgcacc ggctgactcc gaacattttc atttatatgc
    13201 aggaaatgaa agtcaagaag tattgctgaa agaaatggag aattggccaa cattttatcc
    13261 agatacaatg gatggacgtg aaattgcaca ggaaatgctt ttgcactgat aggcaaaaaa
    13321 gaaccgaagg gtttggatcg gctggtcatt tagtcggtcc aaactattcg gttcttttta
    13381 tgaaatcaat ttctttaatc cacggataga ggctgggatg gtctcgccgt ttgccaacgt
    13441 gacttctctg gtttttttat taatcgaccg cacattttgt gtattgataa cataggattt
    13501 atgacaacgg ataaaattcc gatgcatttt ctcgatttct ttgatcttct catagaattg
    13561 taattgggca tgagtcaaat ggacttccag tttatgtggc gctcctagcg aagtagaaaa
    13621 atattggatg tctttgatcg gtaaaaattt gatctcatca ttaaactcta ttttgaagat
    13681 atctttttgg atttgggaca tttgaaaatc ttcgataatt ttgctcagga tatgctcgac
    13741 attttctttt aaatcataaa gatcttcttt gacgatataa tcagtcggtt cgatgttgct
    13801 ttttaagatt ttcatcgaca tttccgtatg ggaagtcaca tagattattt ttcctaaagg
    13861 atctaattca cggatcaatt taccgatcgc tacaccatcc atttcactgt cttctagttc
    13921 gatatctaaa aagtaaatac caaaagaaga agttctgatc ttggctgcat tgagaagttc
    13981 ttccggatca gaggtagaaa tatggacata actgtctaag ttttcaatca tgattctatt
    14041 tttgatcacc gttgataaaa cttctaattg ttttggatcg tcttcacaaa tataaatggg
    14101 aatcatatca ttactccttt attttatttg tatataaatc gtttgagaaa aacgctggtt
    14161 ttcaattgat gtttcaagga aaagataagg attggtcttt gtcaattcat ccaagataaa
    14221 caagccaagg ccttcgtttt ttggatttgt ttttgttgag aacccttttt gtttcaactg
    14281 gtttaacggt aatgtatttg ttgttgtatt gatcacacga atcatcacgg aatgattctt
    14341 cttgagtatg gtcaattcga tttttttctt ctttgcagca ctagctcctt caatcgcatt
    14401 gtccaagata ataccaagca tacgtacaag gtttaccgta tgtttcgtat ccagttcgat
    14461 tggctcagga aaatgaagct ggacgatcaa ccctttttcc tgtgccatca tgagttttaa
    14521 tgacagtaaa ctacggatct caggaacttt catattatcc aacatactta gattggccgg
    14581 aaaaaggttc atcatctggc gtgtaggtaa tatcgtttga tcataataag accgtaatcc
    14641 ttctaagtcg ccttctgaaa tatagctgtg aagagaaaaa agcagatttt ggtaatcatg
    14701 tttgaattct ctcaagtctt gatagttttt ttctagatct ttgatataat cctgttgcat
    14761 cctgacttgt tgctctaata actgatcagc taatttctta ttagagattt tccaataata
    14821 aaccaagatg atcaggccaa cacacagata tagaatgaaa aaactcgtat tcaaagctaa
    14881 gacatcaggt gtttcaccag aaaaacgggt ataaaaaata atgatatagt atgtaaataa
    14941 tatcaatgtc ccgatcgtac cgatcatacg gcggtccaca gtgtgaaacc aagtctctaa
    15001 cgattttttt aaacaaaaag ctaataaagc agataaaaaa gcggataaga caatgtgaaa
    15061 atactgcaat gaatctcctg gttcaataac cgaagatttc aaaacaaaaa aatcgccgat
    15121 gcttgttaag tgatctccta aaagcgacgt caataaccca aaaggaagcg cgtaaaccaa
    15181 ttgttgacgc gaagtcgaaa agaagaaaaa agtaagcatc agcattccta tagcaattag
    15241 agcagtaaaa atccctaaaa tcgaatagac ccatccaatc aaaatacaag aaatcagaat
    15301 gactcccgca aacagatatt tgtatcggat gtgatggtca atcaataaga tacaaattgt
    15361 gtaaatcaaa tattgcaacg ctatggacca taaaaacata aagtacagct cccattgatt
    15421 tttaccaaat tatagcacaa ttggcgtatt atttttttaa tttttcaggg attttgaatt
    15481 cagaactcat accaaagcag cagccagata caaaaaagtc agataagtta gttaattgtt
    15541 taatgattgt ttttttcatg aaagattcct ccaaaattca tcaagtagtt tgtaatagga
    15601 agcaacgtag ctatttctaa aacaatccca agtagcaaat aggtagaaaa gtaagaagct
    15661 gagaaaaata acgtaatgag agaaaaaatt agaaacatgc tgatcgcctt ctttaataat
    15721 gggggacgtt tctcgggttc aatgggattg attgctgtac ctcttgatcc atagcgaagc
    15781 agtaacaaaa agcagctgat taacaaaaag accatcattg ggaaggagat agtcaattgc
    15841 agatgtttca tcaccaaggg aacgccccat aaataaagta gtgtccacag cagacaaacg
    15901 aattcagagt cggaatgcaa tccataagca tatctgcgga tgcagatata agaaagaaag
    15961 gtgattcctg tttccgccca tgagcctgtc accagagaaa agactagaac gaccgctagt
    16021 tttccaagat tattcaataa aacttctaag acaaatttta cttggacata caccatatct
    16081 tcttccgatt tgtctccgct gaataaggct tctaataatt tgttcacaac tttatctttc
    16141 atctcatacg tacctccacc acaaatttta tgctaaaaaa aataaaaaac gtcttttgtt
    16201 tcataaaaag tggttttgac atcaaaaccg tcatgatctc ctaaaaaaat cttcgttttc
    16261 caagaacctt accaaaaaaa gtgacagttt tgatacgaaa agtacttttt atgacaagtt
    16321 tgtgcttttt cgagtgattt atgttaaaaa attactgacc ttataaaagg tgatggaggg
    16381 attcttatgg aaaatgaaca attgtttgaa gtcggcgaaa catatgcatt taaatctgat
    16441 ttatttgaat caccaataaa aggaaaaata gaaaaaatct atgaaaatag tgtgatgata
    16501 cgtgtcatga aatgcgcagt agaagacaat gataccgctc ataatctgca ctggcagtta
    16561 gttgtacgca aatcaagtac aatagaagaa taaaaacaga taaaatgttg agagcataaa
    16621 gaggttgtga gtctcccgtt tagctctgag ttacaaggaa taatgttaaa aaacagcttt
    16681 tcatgtttta tgaatattgc cttgtgaccg aggtgttggc aggagaatac tgtttatttg
    16741 aaaaaagggc tgcgacaaga cttttgtcac agcctttatt ttcatgaata ggagttactt
    16801 ttggctactt ttagagaagt agcttttaag gagcagataa agtcaaggct tttatttcta
    16861 taaatgaagt agtaaaatga agaacagatt ttattacagg aaggatgccg ataattgtga
    16921 tcattgttgc aggaacgatc ggtgcaggga aaagcacact aactgaaatg cttgcccaag
    16981 atttagaaac aaaacctttt tatgagaacg tggaagataa tgaagtattg ccgctatttt
    17041 attctaatcc agaaaagtat acttttttat tacagatttt ctttttgaac aaacgctttt
    17101 tggcaatcaa agatgctttc agccatgatg acaacgtact agaccgctcc atctacgaag
    17161 atagtatgct gtttcatttg aatgcagatt tagggcgtgt ttctgaagta gaagtaaagc
    17221 agtatgaagg tcttttagaa acgatgctga aggagttaga agaaatttcg ccacaaaaga
    17281 aaccagatct actggtttat attcgtgtat cctttgaaac tatgttagcg agaatcaaaa
    17341 aaagaggccg ggaatatgaa cagctagaac aagatcctga actctattcc tactataaag
    17401 aactcaatcg acgatacgaa gagtggtatg aacagtttga tatatgtccc aaaatagtga
    17461 tcgatgggga caaatatgat ttcgtagccg atccggcatg tggagaacaa atcgtacaag
    17521 aaatcaagat gcgggcaaag aaaatgacag aagaggcaga tgcccagtcg atccgctaca
    17581 ctacaaataa cccgaaagca aagggaatct ttacaactaa ataatataaa aactaagcaa
    17641 attgctcact cgcagatatt tctctgtgag cagcatcttg cttagttttt ttgattcatt
    17701 gagatcaaat agtgaaccga ttcgtatgat aaaattgcca taaatgaaat gtctgagtga
    17761 gggccatgat caaaaataaa atggaaacga tcaaaatcga atgggcgcca aacagatgat
    17821 gacagaaaac aaggacaata gctcctgcga taaaggcaag aacgacactt agatagtgga
    17881 aaaatcgatc aaaatctgct ttttctttag aaatacaata atcatataag ttcgttgcta
    17941 ccccttttaa atttccagtt gtaaataaat tagtgtaggc acgcccgttg attttatcaa
    18001 aagctaccca ctggatcgat gcagtaaaag caatcaaaat cgttactaat gcgggatgct
    18061 catggacggg tgtcaaacta acaaagaaaa aaatcactgc ttcataatat agaacaaaca
    18121 agcgccagaa atgtttttct ttttttctaa aataatcaat tagatacttt gttagaagaa
    18181 tccctaagaa aaagaaaagg gtagacaata cttttttacc aacacttagc cattctccgt
    18241 caaatgcttg gatgatcgat agaatgatat tgccagtttg agcagaggca aaagcaccat
    18301 attggatata agtataactg tccatggctc ctccgataaa tgtcagcaaa agtcctattg
    18361 tacgcccctc gtgaaaggaa gtctgcattt ttttcatcac cttttatgaa tatttttgtc
    18421 ttccttatct tagcataaac gattgaaaaa aaggaaaaat tttcagaaaa ctaaaatcat
    18481 atcgcttccc attttcattt ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga
    18541 aactttaaat aaccaacttg aaataaacaa attcaaagaa gaaagatggg aaggaaatca
    18601 atcagttgaa tgaaaaagaa attctccgat tacaaaaaga attgaaacgc tgggagaaca
    18661 agtataaaaa gcttcaacaa gtctttcggc tacaagaaga aatcattgct acctatgaag
    18721 cggcttatgg cgaagcaccc ggacttattg aggaagaagt acaagtaaaa gaaagtgaag
    18781 gaaacagctc tttctttaaa atgatggaga aacgttccta taatcgcttc aatcaagaac
    18841 aaaaagcagc tatcacatat gatatgaaga aacatcttcg aatcatagca ggagctggga
    18901 gcggcaaaac gcaaacaatc tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag
    18961 acgaagaacg catactaatg atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac
    19021 gagtagataa tttcagccag agaaaaaccg ctgtccatat cggaacattc catagtatct
    19081 tttttaggat atacaatgaa atttgtcgaa aatttcctga agtagcaatg caaggaatac
    19141 aaggagactt ttcaaaggat actgcacaaa aagtgaatgc tgttttgcaa cagctgatac
    19201 gtaaatataa tctttatata tttgatcaat atggagaaaa aacgattgct tctcggttag
    19261 actattggca gaatatgaac ctttcagtag aagaaatggt tcagttagtt aaagaaaaat
    19321 atgattccat tgataagaat gcacgacagc cgatatcaga acgactgtat ggattgctca
    19381 cggaactcca agaacaaaaa agaagccagc aattgctaga gttcaatgat gtactccaaa
    19441 atctgaaaag cgctttggag aatgaggaga tccagcatta tatcggccaa aaatatgatt
    19501 atctattcat tgatgaattt caagatacta atccacttca atgggaaatc gtgaaacgga
    19561 tgacaaagga aaacaggatc aagttgattg ttgttggaga tgatgatcag agtatctatg
    19621 gattcagagg atcagagcca gcatacatca agaactttga aaaagaatat cctaccaaaa
    19681 ctcttttctt attaactaat tatcgttctc gggcagctat tgtccaagga gcaaatagat
    19741 tgatcagcta taataaaaat gatcggatac caaaggcgat gattccagca caaaaagaag
    19801 caggtgttat ggaagcctat ttgttttctg atactaaagc agaaagtcag tggctaatca
    19861 aacaaattca gacatttatt caaaaaaatg ggaaatataa ggaatcaatc atattatacc
    19921 gatcagcttc acaaacgcag cagcttgtcc aatctctgct gaaaacgaat attccttttg
    19981 tactggaagc agatagtcca tatgaaggta tctttggtat caagtctttc caacatttct
    20041 atcaaaaaat tgttcattgg caaacagcac tgaatctgca aaagaaaaat caggcctacc
    20101 agcaattgct ccggcagctg atggcagact gctacctgaa aaaaagtgaa tgcgatcaat
    20161 acttttctcc taaatatcaa aatatagccg ttgcagatta tattttgtcg ataagcccca
    20221 atttaaaatc gaaatctgca gaaatccgag agtttgagga agcgttgcgt aaaacagacc
    20281 agcatcaaga aatctattcg cttgttcaaa gctatcttca tctgccacgt atagctaagg
    20341 aaatcgacca gagtgatcaa gaatggatac gagaagaagt aaagcagcat gctactttca
    20401 gaagactcca agccttatcg caagaaaccc aagcgacagc taaagaatta aaaacccgct
    20461 tgattgctta cagacaagga aaacttgatg cactatgtat ccagagcatt cacaaaagta
    20521 aaggtcttag ttaccgaaat gtctttttga ttggttgtaa cgaaggaagt ttgccttata
    20581 acggagcgac agaaaagaaa gtaatcgact ttaatgaaat aaaagcagaa ccagtaacga
    20641 cgatagaaga agaacgaaga ctattttatg ttgcgatgac tagagcaaga aatcgtctgt
    20701 atctatgtgt acctcaaatg aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag
    20761 aaaccgggag tcgtgtgaaa aaaggaaagt gatagaagag agggtgtgac agaagtggtc
    20821 agtttcaaga aagaagaagg aatgtacgaa aattgcttct caaatttttg tgaaatttca
    20881 gcttattttc gaagaagcta tttctggaac acagtttatt cggaaaaggg aggtgtgaca
    20941 agaatttt
//
LOCUS       DACCWK010000042        20947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCWK010000042 DACCWK010000000
VERSION     DACCWK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.042"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            598..1287
                     /locus_tag="IVW65_001475"
     CDS             598..1287
                     /locus_tag="IVW65_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900358.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="IVW65_001476"
     CDS             1274..2482
                     /locus_tag="IVW65_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7900359.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="IVW65_001477"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="IVW65_001477"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7900360.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IVW65_001478"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IVW65_001478"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7900361.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="IVW65_001479"
     CDS             complement(6585..7280)
                     /locus_tag="IVW65_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7900362.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IVW65_001480"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IVW65_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7900363.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="IVW65_001481"
     CDS             complement(8479..8670)
                     /locus_tag="IVW65_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7900364.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="IVW65_001482"
     CDS             complement(8691..9581)
                     /locus_tag="IVW65_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7900365.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="IVW65_001483"
     CDS             complement(9568..10335)
                     /locus_tag="IVW65_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7900366.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IVW65_001484"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IVW65_001484"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7900367.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IVW65_001485"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IVW65_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7900368.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IVW65_001486"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IVW65_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7900369.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14951..15289
                     /locus_tag="IVW65_001487"
     CDS             14951..15289
                     /locus_tag="IVW65_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900370.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="IVW65_001488"
     CDS             complement(15457..16215)
                     /locus_tag="IVW65_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7900371.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="IVW65_001489"
     CDS             complement(16233..17051)
                     /locus_tag="IVW65_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7900372.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IVW65_001490"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IVW65_001490"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7900373.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IVW65_001491"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IVW65_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7900374.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="IVW65_001492"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="IVW65_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7900375.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="IVW65_001493"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="IVW65_001493"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7900376.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5988 a         4263 c         3838 g         6858 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttg
//
LOCUS       DACCWK010000043        20362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCWK010000043 DACCWK010000000
VERSION     DACCWK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.043"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            291..1430
                     /locus_tag="IVW65_001494"
     CDS             291..1430
                     /locus_tag="IVW65_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7900377.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="IVW65_001495"
     CDS             complement(1497..2072)
                     /locus_tag="IVW65_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7900378.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="IVW65_001496"
     CDS             complement(2077..3051)
                     /locus_tag="IVW65_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP7900379.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="IVW65_001497"
     CDS             3334..4302
                     /locus_tag="IVW65_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7900380.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="IVW65_001498"
     CDS             4415..4693
                     /locus_tag="IVW65_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7900381.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="IVW65_001499"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="IVW65_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7900382.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5159..5905
                     /locus_tag="IVW65_001500"
     CDS             5159..5905
                     /locus_tag="IVW65_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7900383.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="IVW65_001501"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="IVW65_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7900384.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="IVW65_001502"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="IVW65_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7900385.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="IVW65_001503"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="IVW65_001503"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7900386.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="IVW65_001504"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="IVW65_001504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7900387.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="IVW65_001505"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="IVW65_001505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7900388.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="IVW65_001506"
     CDS             10642..11184
                     /locus_tag="IVW65_001506"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7900389.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="IVW65_001507"
     CDS             11207..12763
                     /locus_tag="IVW65_001507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7900390.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="IVW65_001508"
     CDS             12777..13679
                     /locus_tag="IVW65_001508"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7900391.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="IVW65_001509"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="IVW65_001509"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7900392.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="IVW65_001510"
     CDS             15122..15544
                     /locus_tag="IVW65_001510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7900393.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="IVW65_001511"
     CDS             16013..16243
                     /locus_tag="IVW65_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7900394.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="IVW65_001512"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="IVW65_001512"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7900395.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="IVW65_001513"
     CDS             17654..17830
                     /locus_tag="IVW65_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7900396.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="IVW65_001514"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="IVW65_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7900397.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="IVW65_001515"
     CDS             18325..18708
                     /locus_tag="IVW65_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7900398.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="IVW65_001516"
     CDS             18877..19761
                     /locus_tag="IVW65_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7900399.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19878..20111
                     /locus_tag="IVW65_001517"
     CDS             19878..20111
                     /locus_tag="IVW65_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7900400.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="IVW65_001518"
     tRNA            20199..20279
                     /locus_tag="IVW65_001518"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="IVW65_001519"
     tRNA            20286..20357
                     /locus_tag="IVW65_001519"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3567 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat ac
//
LOCUS       DACCWK010000044        20248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCWK010000044 DACCWK010000000
VERSION     DACCWK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.044"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            34..471
                     /locus_tag="IVW65_001520"
     CDS             34..471
                     /locus_tag="IVW65_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900401.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            566..1450
                     /locus_tag="IVW65_001521"
     CDS             566..1450
                     /locus_tag="IVW65_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7900402.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1674..4841
                     /locus_tag="IVW65_001522"
     CDS             1674..4841
                     /locus_tag="IVW65_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900403.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4861..5406
                     /locus_tag="IVW65_001523"
     CDS             4861..5406
                     /locus_tag="IVW65_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP7900404.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5434..5889
                     /locus_tag="IVW65_001524"
     CDS             5434..5889
                     /locus_tag="IVW65_001524"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP7900405.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5886..6644
                     /locus_tag="IVW65_001525"
     CDS             5886..6644
                     /locus_tag="IVW65_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7900406.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6730..7470)
                     /locus_tag="IVW65_001526"
     CDS             complement(6730..7470)
                     /locus_tag="IVW65_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900407.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7531..8784
                     /locus_tag="IVW65_001527"
     CDS             7531..8784
                     /locus_tag="IVW65_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7900408.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9106..9333
                     /locus_tag="IVW65_001528"
     CDS             9106..9333
                     /locus_tag="IVW65_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7900409.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9456..9782
                     /locus_tag="IVW65_001529"
     CDS             9456..9782
                     /locus_tag="IVW65_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7900410.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9850..10563
                     /locus_tag="IVW65_001530"
     CDS             9850..10563
                     /locus_tag="IVW65_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7900411.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10873..11724
                     /locus_tag="IVW65_001531"
     CDS             10873..11724
                     /locus_tag="IVW65_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7900412.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11845..13710
                     /locus_tag="IVW65_001532"
     CDS             11845..13710
                     /locus_tag="IVW65_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7900413.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13758..15167
                     /locus_tag="IVW65_001533"
     CDS             13758..15167
                     /locus_tag="IVW65_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7900414.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15275..15976
                     /locus_tag="IVW65_001534"
     CDS             15275..15976
                     /locus_tag="IVW65_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7900415.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16135..16614
                     /locus_tag="IVW65_001535"
                     /pseudo
     CDS             16135..16614
                     /locus_tag="IVW65_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            17462..18037
                     /locus_tag="IVW65_001536"
     CDS             17462..18037
                     /locus_tag="IVW65_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7900416.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18037..18477
                     /locus_tag="IVW65_001537"
     CDS             18037..18477
                     /locus_tag="IVW65_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7900417.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18493..19194
                     /locus_tag="IVW65_001538"
     CDS             18493..19194
                     /locus_tag="IVW65_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7900418.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19682..20107)
                     /locus_tag="IVW65_001539"
     CDS             complement(19682..20107)
                     /locus_tag="IVW65_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900419.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         6764 a         3379 c         4298 g         5807 t
ORIGIN      
        1 acaatatcaa aaaatgcacg tagaagtaga taaatggagt ggttttgcaa atttctttta
       61 tttctagaag ttattcacgt atacggattt gtttttctca caaataaaaa ggcaggaagt
      121 acaatggatc atttgtatat aaaatatatt catcaatttt ccgttttggt gttaggcaca
      181 ctaattatag cggggattgt cgcatttatt attcaaggcc gtgacatatt aacagtacat
      241 caagagtttt tcagttatgc atttggtatt cctctgatcg ctataaggtc tgataacaaa
      301 aagtggaaga aacgctatta cattctcttg ctatttttgt gtttttatct gccacctaag
      361 atttttactt tattaggtat acaatgccta tcattttctt ttatttccta tatcattgtt
      421 ttaattttcc tcagtttcat agtatggatg ttttatcttg tcaaaaaata aatgaatgtt
      481 tcatgaacag tacttcatca cagcctaatt tttttcttta tgctatcgtt atattaaaga
      541 aatcaatcag aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa accccagttc
      601 aggcggtgaa caagcaaagg aatttgagca attagcgata gcaaaattgg aatcggtgtt
      661 tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact ttactcgcga
      721 agcggctacg gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa cagtcaacga
      781 aggaatcagt ggaatcgctg aacaagaaca tcggccaaat ttcggatttt tcccattggg
      841 tacggtgaat gacttggctc gagcattagg gataccgcta gaacctgagg aagcgatcaa
      901 tcatttttcg attgaatcag tcaagccatt ggatattgga aaaataaatg acgactattt
      961 tatgaatgtc gtcgcaattg gctctatccc tgaatcaatc aatgatgtag atgcggagaa
     1021 gaagacgaaa tttggaaaac tagcttattt catgtcagga atcaaacagt tagcttccac
     1081 acaatcctac tcgtttcacg tagaagttga tggaaagaag gaagaaattg agagcagtac
     1141 cttgctgata ggactaacta actcagtcgg cggttttgaa acattacttc caaatgcaaa
     1201 agtggatgat ggtaagctcc atttggttta tttaaaagat tcttctttga tcgatacgct
     1261 taaaacattg ccagacctat taaaaggagt agatgaatct acgaaaaatc tagcctacct
     1321 tacttgtgag caattagttg tgtcattagc tgatgaggaa gaattgacga cgaatgttga
     1381 tggagatgaa ggggataaat tacctgtgac aatcaaagta ttgccctcac atttacaggt
     1441 ttattgttaa aatggaggtt gtgaccgagg agttggcggg agaacaccgc ttgttcggga
     1501 taagggactg tgacaagact tttgtcacag cctttttttt gtacaaaaaa gaaatttaca
     1561 cctaatagaa aaatttttcc ataaaaatac atattttaaa tatgaaagta caattggaaa
     1621 tagattaaaa cgataaaata aaattgtcaa aacgtcagag aggatggtgg agaatgaatc
     1681 aatgtgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg atggtgacat
     1741 ccgtttttgc cgatgtagag aaaacaaacg aatcaacgca atcagagaat caaagttttg
     1801 acaagactac tttttcagaa gaatcgatta cttctgaaac tacagaatct caaagtgagg
     1861 cttctactaa taaaaggcaa gaagaaaata agacaagtga gacctcagaa gtagctgaac
     1921 aaacgtctga tagcagtgaa gcggcactgg caagagctgt ttcagcggat gggtacagcg
     1981 aagctgccac aacagaagaa ctggcgcagc ttctcgcaga cgaatcagta actaagatcc
     2041 gactgattca gcctttgact ttagacaggg aattagagat caaacgagat atcgttattg
     2101 attttggtgg attcgctcac aatttcggca cacaccatat ttatatcaat gagatacctc
     2161 agtcgatcga gttccagaat tttaaaggaa cagctgcaca tccaggtgga ttgacaccaa
     2221 atatcgatgg aaatgccatt atcattgctt atatgaccga tttttgggga agcagatatc
     2281 attttacggg agaaataaaa tttaccggga cacttgattt gtatgatggg tccaaattag
     2341 gactgattta cgctcctcgt gcaactgtca cattagatgg tgtttccggt gtgttggatg
     2401 tccaaccagt gaaggaagga ctgaatgccg ctccgggtaa agcctatttt tgtagaagct
     2461 atcaattgaa tgtgatcaat ggtagtcaac tttatggacc gtatctaggg aaattctacg
     2521 gttttctaga ttcaggagat gagactggta gtggaaaaac agcgccagga atccatatct
     2581 taagcggttc aaaggtctct cttgattatg atcgggcaga tggcgttagc gatgaaggag
     2641 aggcagtcga tacgctacct ggtaatgtca tgtttgaagt cagtggttcg ggaagcgaat
     2701 tttcggtaaa tacaaagatc aatattacca atgacaacaa tcgcggaatc atccaaatga
     2761 gaggttcagg atcgaaagtc actgtatcga atggtgggaa aatcaccatc aatacagcaa
     2821 ctacgggcgg atttcgcttg caaggagatt ctagtcaaat ccatgtatca agtggtggac
     2881 aaatctcggt caaaatggct ggagatgacg atcaacccgg gaacaatggg atgcgttttg
     2941 taggaaaagg cctttctttg atcgtagaag gagccggatc agttatcgac atcgataagc
     3001 agagtgggcg ttccagcgca gttcgttttg agaatggtac gcaaaagctg acagtaacca
     3061 atggaggctc attgagggta agaaatacag gtgatggcaa atcttacgtg aacagactca
     3121 atcaaggaaa tcaagcgatc caattttatg acgcttctgg ttttctagaa agtccaggtt
     3181 cagctgattt tacagtcact ggagaaaaat caaatatcga tatacgcgca gattccggag
     3241 caactgtaga tacaactgga gatattgatt taaattttac ggcaggtgaa aaaacttatt
     3301 tagtcatggt cgggaaaaca ggggaagata agaccggcat catcagtggg gatgtgttga
     3361 atgtggactt agaatctcct acctattttg atttccaaaa caaacggatt ggaagcgaga
     3421 ccaatggcat aggcggctgg gtattccaag gaaatggaga ttcttcttta aagatcgccc
     3481 attcagaaat cgctttatgg cgaaaaacag gcgcaacatt cgataacttt gaaaaagacc
     3541 cggattattt ttctgatatg acagacttat cgatcaatgg gaccgactta ggcaatttcg
     3601 tatcaagccc tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc ggaatgaaaa
     3661 attataatcg aatcagtgcc aataatcagc tcccgatgat tgaatcctta agagtgccaa
     3721 cgaatgcaga taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc gaggcaaagc
     3781 caagagatgc ttggacaggc gaagtagaag tgactttacg tattacttac gcagatacga
     3841 cgaaatcatc tgtagaaatt actgctctca cgcaaggaca aacagatgaa aatgaagggg
     3901 caggatataa cccatgggga gaaggcgaac aaggcggctt gttcgaagtt caagtaccca
     3961 atgatgagag attaaaaacg gaggatcaag tgcaagttgt tgctgctaaa aaagtaagag
     4021 gtggccaaga agtgacagaa ctggactctg ccaaaacgac agtcgatgtg attcctcctg
     4081 aacctgctaa tttaagtacc acattgatca atgcagcttc tcgcacgatc acaggtacag
     4141 gtgaagcagg agccttggtt tcattgaaaa aaaatcaagc tgttttgtca gaaacaacta
     4201 ttgatgatac aggggaattt gttctttcta tcccagaaaa tcaacttttt gcaggagatg
     4261 tattagaaat tgtattaaac gaccaagcag gagaagctac tgctaagggt gtgcagaaca
     4321 agccaagtac caacgatgaa attgggaatc acaatccaag tgatacgcca gttgtttatc
     4381 atgatgcccc gccttttgca cctgctacga agatcgtagt agaaggtggt ctctcatttc
     4441 ttgccccttc caaactcgat tttggtcaga tcaaagcaac tggtttgaca caagaagcat
     4501 ttggccaaat ggatatacag gaaaaattat ggatttatga tcagcgagaa acgaaaaatc
     4561 cgtggatctt aacgataaaa ttatctcagc ctttcacaaa tgatgcagga ttttctatgc
     4621 aggatgtcct atatttcaag aaaggagagg agtatcaatt agtgaatgaa gaagaactgg
     4681 aaattgtcag aggacaaaat tcaacgaatg tgcaagcaga ttattcagaa tatttaaata
     4741 acggtcgggc gtttaaactt gtcttaacca aagaaaatca gatagctggt aattactcag
     4801 cagagataac atggacatta gcagatgcgc cgacagaata aagatcaagg aggatgattg
     4861 atgaatcgaa tatggaataa aagtcatcga aacaactgct ctttacttat gatccttctc
     4921 cttggattca tcctttggca gacacctgtc catgcctccg aattcaattt tgcggtagat
     4981 tttgaattac cggctaatca gattgataag aacaaaacat atataaatgt aaggatggag
     5041 cctgggaaaa cacaggaaat cccctacacg gtctttaacc gaacagataa atcagtaaca
     5101 gttgagacat tgatccactc cgctacgacg aattcaaacg gtgtgatcga atatggagaa
     5161 tcaaaaagta aatttgatga aacattaagt tatgatatga aaaacttagc aaaaacagtg
     5221 ggagaaataa ctgttcctcc tcaaagtgcg gtcactgaga cactttcact gaaagcgcca
     5281 gaaaaagagc tggctggtgt ggtcgcagga gggatcacgt ttcaagaaaa aaaagagaac
     5341 gtttctaaca aaagtacagt tggtgtcgtc aatcagtttt ctcaagtgaa agcaatcttg
     5401 atttgaaata gcgaaaaaga gtcttcccca gatttgaaat taaaaaaagc ttatgcggat
     5461 caaagcgatg gaagaaatgt gattaaggcg aatcttcaaa atccaaatgc ttcttatatg
     5521 ttcggcttga ctacatcggt tgaaatctac aaaaaagaaa caaaagagcc ttacctttca
     5581 ataaaaaaag aaaaaataga tgtagcaccc aatacaaatt ttgatttact tgtcccatta
     5641 gaaggaaaaa aattagctcc gggacattat caagcggttg tgaagggtgc ttggaaagaa
     5701 aaacgctgga atctccatac cgattttgaa ataactgatg atgcagcgga ccaattgaat
     5761 gaaaaggata ttgatttaaa aaatcagcca gaaacatttg actggcgatg gctgatactt
     5821 gttggagtca ttcttattgg cttatttgct ggattattta ttttaaaaga aagaaggaaa
     5881 aagaaatgaa aaaaacaatt gcattaagta ccgttctagc tggaagtcta ttattatgta
     5941 cacaaagtgt ttttgctgag aataagagct cagccactac agaaggaacg atttctgtga
     6001 ccaaaccaga tgctgcaaat cctgaagacg ttgttccagt tcctcctgtt gatccaaatg
     6061 atcctgatac tccttttaca cctgacgaac caaatcaggg aacatccgga ttactgacga
     6121 ttgaccaagc tccagatctt gattttggaa caatcaagtt aggtacagca aaaacagtat
     6181 atgctgcatt acaaagcgga acggacagta ctggagcaca aacacaagta cagaatttcg
     6241 tacaagtaac ggataaaagc ggaaattatg ccggttggaa attatcagtc aaacgaacag
     6301 aatttattgg tacaacatct gatacgaata aactaactgg ttcaaaggtg aaattcaaaa
     6361 acgccgtgat tcgaaatagc gcaactaata gcagtggaat gccatctacc gtttctggaa
     6421 ctggcgctga aggaatcgaa atcccagcag atcaggcagt agatctagta actgcaggag
     6481 caaatgaagg gattggcaca tggatttatt cattaggaga gaatgtggaa caagggagac
     6541 aatcagtgga attatttgtt cctgtatcca attatgctgt agacaactac gtttcaacat
     6601 tcacatggtc gttaacagat gctcctacag aagaggaagc gtaaaatcat atcaacgatt
     6661 tcagggagtg gagccgattc aaggcccact ccctgaaatc tgctatgctt acgtctcagc
     6721 tcttattcat caactcgaag cagttttttg gaagtcatgg aaacgttcct ttgtcttttc
     6781 aaagatcttt cgaccaacga gtaaggagtc accgttgaaa aattctaaca cataatgagc
     6841 agaatcaatt ttctgcaatt gcgataagtt gatacagtaa gattttaaaa gtaaaaagta
     6901 agcatacttt tctaactctg tcttcaattg ttttaaagaa gtattcacta atttatcttc
     6961 tgtagttgtt ttgaccataa cttggttttt caaacctttg atactttcaa tatataaaat
     7021 ttcccgagga tacaggacga gacttccacc tttttgatca ataaagattt tatcgacatt
     7081 tacgatctgc tgttctttcc aattgatctg ttctagcact tgacgaattt gttcagataa
     7141 attcctgtct tttctgatat atccaatcgg aaggattttc cgattgatga tctctactgc
     7201 aaaattcgta taagcggtga aaaaaacaat attactatgt gcattttttt tacgtatttg
     7261 ttcggcgaca tcaatacctg tgagtgattg cttcaagtcg atatcgataa agaaaacatc
     7321 gtgattatca atagtgccca actggatcaa ctgattgatt tgagagatag aagtgagctg
     7381 cacgatattc tcgatatgat gattgaccaa ttctctgatg atccattcgc tgtgaaactg
     7441 gttatcttct atgataaaat actttttcat atgcaggctc ctattaaaaa aatatgtgta
     7501 cattttgaaa caaaacaatg gaggaataag atggtcgcaa cagtgacact ttatttgacc
     7561 tttactttga ttttagattt aggtatctat ttattctttt cgaaacaact gctagtaaaa
     7621 aacagctttg gttgtatagc tttgatggtg gtgacacttt tacttcctat ccctaaagtg
     7681 agctttcctt acctagttgg cttattattg atgtttcttt tttattttac tacgaaaaac
     7741 ctgtttatca gctgtttgat cagtaatctt ttttttgcta tcactggact cagctttttt
     7801 ctggtttttg atctgatgga ttatttagga aagagacatc ttggaacagc aagttttttt
     7861 gcactactta tcgcatttat tttctatcta ttagtaaaaa gtatagacaa acagacgaga
     7921 tttctcaact tgctttttac ttatccaact gaacactatt ttggaggtat ttgtatttta
     7981 ttaagctggg gcgtgattta cttggtattg ggaagtgttc acttccgctc atctaattat
     8041 ctgcttctat ctcttttgtc gatagtcatc agtttttttt ctttgttttt ttccattatg
     8101 ttgatcagtt tccaagtaaa agaaagacaa catcaggaat cgcttcaact gtatcaaata
     8161 aatgaagaat attaccatca tctagaggaa ttcaaacatg attataaaag tctactgttt
     8221 tcattaaaaa ttactcttca ggaaaataaa aaagaaacgt tggaattttt agaaatatta
     8281 gaagatcatt ctgcagctat ttttgaaaaa ccgcaaatac aacagctaac aaatattgta
     8341 tccccagctg tcaggggtgt ctttttaaaa tttctagaaa aagcagaatc ggagaaaatc
     8401 ccgatccaag tagttattcc tgtcgaagtg aaaacaatcc cgattgatct ggttgttttt
     8461 attcgctgtt tatctatttt tatttctaac gcattcgatc atcgtgtagc cgatcagagt
     8521 ccgattacga tcaatctgct ggaagaaaaa gagggatcac gttttagtat agcgaacaag
     8581 gcaaatccaa cgaatttgac gctatcggag atggtcaaaa gaggaaaaac ctctaaaaaa
     8641 aatggcgggt taggtctata cagtgcgaaa aaaatgttgg atgtttacga aaatgcagaa
     8701 ctaaaaatcg aattttcaaa acagaatcac tatttttttg tagaaatgta tctggcatct
     8761 agaaagagca aatcagccta ctgacttact gtcaaaggat aagtttgagg aaagtctaca
     8821 cggagataga gacaacaaca cagcatattc aaaagaaaca gaagcgtgat gaccgttgtc
     8881 acccttctgt ttcttttgct atgtagttaa acaaagtagt tgacacttcc gctacttagt
     8941 attagtacga aagtatactt catgatacta agtagataag aagtgaaaag cggaacccac
     9001 gatcgtcaaa atgctaactg ctttgcttac accagataaa gggaagagca atatcgctta
     9061 ttttaggcgt ggagaagcaa gcaaatccct acgatcacaa aaaaaatgct gacccagcag
     9121 ttaagagagt tagaacaaga tgaaagtatc catcgaaaag cctataacca agtcccgcct
     9181 aaagtagagt actcgctcac cgaagaagga aaatcattaa gagagattct agtagcgttg
     9241 tctgtttggg aagaagaacg tatcgaaaaa caaaaagcct caagggaaga agacacgcta
     9301 ctgcatacac atggtggcta cctaaattat tgattttcta tgacatttat tttaaataaa
     9361 agaaaacagg agttttttta aaagcatcat ctatttttta tgtaaaaaaa tggtatgatt
     9421 aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa tagtatggaa aaagtcaacc
     9481 agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg gctatctcta
     9541 tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg gaagaagtaa
     9601 aagaaacaca attagaacga atcaaagaag aactggcaga tgtattgatc tattcataca
     9661 tgatggcaga caaccttcac ctggatctag atgagatcat tgaagaaaaa ttgatcaaaa
     9721 ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac acagagttgt
     9781 agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat gagtctggag
     9841 ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat cctcttgttc
     9901 ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga ttattttgtc
     9961 agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct gaaaaagaat
    10021 aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac agaaggggtc
    10081 atgaggtcgc aaatgagagg aacagatcta ccagtgattg ctagtatcgc agtcccttca
    10141 gtttcaatca atttaccggt tttcaaaggc ttggataata cgtcactact ttacggagca
    10201 ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc aagccatcgt
    10261 gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt tagagatggg tgccaagatc
    10321 tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga aaaagttgct
    10381 cctacagata cccaattact gaatgaagtc gagggaaaag aaattgtcac actaattact
    10441 tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga aagtgttaca
    10501 tcgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac aaaaacattt
    10561 taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat gaatcgataa
    10621 ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag gtgcatctct gatttggttg
    10681 ttttctagat agacttgtgt tccagttttt ttatttttta tatgaaaagg cttgcataaa
    10741 taaaaattcg atggtatgat gattttgaat aagaaatgga ttgttactgt acggcaggca
    10801 aaaccagaat tcagctaaga aacagtaggc gaattctagg tttttttatt tttcttgaga
    10861 gggcgaagca aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat ttcgaaaaat
    10921 ggattcgggg aagaagtcgt ttgtatggga aaaggactag cttttcaaaa aaagattggg
    10981 gacgagattt cgccagaagc ggtccaaaaa gaatttgttt taagagattc ttttgctaaa
    11041 aaccaattcc agcagctgat ggctgatatt ccggcagagg agatcgaatg ggtcaaacaa
    11101 gtagtcgaac tggctgaaga taaactaaaa gttgaatttt cgcctaatat ttatctgaca
    11161 ttgaccgatc atcttcatta tgcgatcaca cgagcaaaag aaaatatcca actgcctaat
    11221 ccattattat ttgaaacaaa aaaattttat ccgaaagaat atcaagcagc gaaagaggct
    11281 cttttgttcg tgaaacaaaa gacgggtgtg gaactttctg aggatgaagc cggttttatt
    11341 gcctttcatt ttgtgaatag ccaacaagga aacgaagaca tgcaagtgac gatgaccgct
    11401 accacaatgg taagagatat actgagtatc atcagtaaat tcttcggcat cgtgttcgat
    11461 gaagaatctt tgaattatca gcgtatcatt actcatctgc agttttttgc ccagcgttat
    11521 ctcaaagggg aaggatctga cgagcaagat gaatttctct acgcattagt tcaaggaaag
    11581 tatccaaaag cgtttcattg tgtcgaacgg atcaatgagt atcttcgaca aacgcaaaaa
    11641 caggaaatgg ggatcgcaga gcagatttat ttaacgattc atattcaaag agtagtaagt
    11701 gagaaaaaaa gcatccaatc ataaggattg ttactgtatg gcaggcaaaa cctggattga
    11761 aaaaataggt tcacgaatgt ttgtgactgt ttttttcaat ctgggttttt attttttaaa
    11821 taaaaaagaa agaaggaaaa aacaatggat aatcaagcag ttggtcgtcg tgtatgggaa
    11881 gcggtaggag gacaaaagaa tgtcaaaagt ttagtacatt gtgctacgcg gttgcgattc
    11941 agattaaaag atgaatcttt agcagatacg caaaagttaa aagaagaccc ggacgtgatc
    12001 caagtcgtac aaagtggcgg tcagtatcaa gtagtgattg gcagtaatgt agcagatgtt
    12061 tatcaatcga tcgtggatga ggaaggtctt gcagtttcgg aggatggaaa agaagagtcc
    12121 aaaaatattt tgaatcgtct gatcgatatt atctctagta tatttacacc ttttctaggg
    12181 gcgatggcag ccgccggggt attgaaagga ttcttatcat tggcaacggt gatgggctgg
    12241 ctgacagctg atagcggtac ttatcaaata ttgtttgctg cagcagatgg tgtctttacc
    12301 ttcttacctg tcatgcttgc ttttacagca gctaaaaagt tcaaagccaa ccagttttta
    12361 gcagtagcaa ttgctatggc acttgtgtat ccagctatca cagcagttgc tggtgcggga
    12421 gaagctatca gtttctttgg gatcccagtg attctttcac catctggtta tacttcttct
    12481 gtcataccaa tcattctagc cgtatgggtt caaagtaaat tagagccatt cgtaaaaaaa
    12541 gtgattccgc aatttctgca aatgatttta gttccgcttg tcgttttagt agtgatggtt
    12601 ccattaacat ttcttgcttt aggaccaatc gggacggttg ccggtaatgc tttaggcggt
    12661 ctcttcaact cgatttatgg atttagtcca atcgtagcag gtctaatcat gggaagttta
    12721 tggcaagtat tcgtgatgtt cggcatgcat cggggattcg taccgatcat gtttttgaat
    12781 atcgaacaat atgggtttga tgtattgatg ccaatgcttc ttccagccat cttggcacaa
    12841 ggtggggctg ctttagctgt agcattgcga acaaaagata cgaaacttcg agcattaggg
    12901 atttcttcta cagtgacttc gttatttgga atcactgaac caactgtgta tggtgtgaca
    12961 ttacctttga agaaaccatt cattgccgct tgtatttctg gaggtattgg tggagctatt
    13021 atcggatttt ccggagtaaa agctttctcc agcagtttgg tcagcttatt gacgattccg
    13081 actttcatta atacggtgga tggtgtagag tcaaatgtga cagtagcagt aatagctaca
    13141 ggaattgctt ttgttcttgc attcgtaggt acattgattt taggatttga cgaacaaaca
    13201 caagacaatc aattagaaaa caaacatgcg aatgcaggag aaccgattac ttcagcacgg
    13261 cataccttaa aaagtccttt aacgggtaaa gtactgccat tgagtgaagt accagaccaa
    13321 gttttttctt ctggcgtgat gggaaaaggt atcgctattg atccagaagt aggagaacta
    13381 gttgcacctg cagacggaga gatcacgact atttttccaa ctggacatgc tgtagggatc
    13441 acaacgactg acggagcgga gattttgatt catatcggga tggatacagt agaattaaat
    13501 gggaatggct ttgagatctt agtcaaacaa ggggatctcg tcaaagcagg agatttattg
    13561 attcgttttg acatcgaagc aattagagca gctggctata gcgtaatcac tccagttgtc
    13621 attacgaata ctgatgcatt tgccgatata cttgaattag accaaaaaga gattattgca
    13681 aacgaagatg ttctggcaat tgtaaaataa ggaggaaatc aggatgcaat cacgtttttc
    13741 agaaaaattt ttatggggtg gagcaactgc tgccaaatca attagaaggg gcttatgatc
    13801 gagacggaaa aggactatcc gttgcagatg ctatgcctgg aggtaagcag cgctttgcga
    13861 ttatcggcag tgaagaattt gactggacga ttgatcaaga aaaatatatt tacccgaatc
    13921 atcgaggaat cgatcattac gatcgtttca aagaagatct tgccttgttt gcggaaatgg
    13981 gattcaaatg ttatcgtttt tcgattgcct ggacacgaat ttttccaaat ggagacgaag
    14041 gtactcctaa cgaagcggga ttagaatttt atgatcagct gattgatgag tgcttaaaat
    14101 atgatattga acctgtgatc acgatttctc attacgaaat gcctcttcat ttggcaaaag
    14161 aatacggtgg ctggaaaaat cgaaaactga tcgacttcta tgaacgattt gctcagacag
    14221 tattagagag atacagcagt aaagtcaaat attggatgac tttcaatgaa atcaattcgg
    14281 cctttcactt tccagcttta agtcaaggat tagtaaaaag caatggagca ggagagtacc
    14341 aaaatatttt ccaagcatgg cataatcaat tcgttgcaag tagcaaagcc gtaaaaatcg
    14401 gccacgaatt gcgttcggat atccaaatcg gctgcatgat tatctatgca acaacttaca
    14461 gcatcgatgc caatccagtc aatcaggcag caacaatgat ccaaaaccaa gaattcaatt
    14521 ttttctgcac ggatgtccaa gtacgtggag aataccctgc atacacagcg cgtacccata
    14581 aaaaatatgg agtcgatccg gaaaaattag agcaaacgga aaaagacttc cagttgctaa
    14641 aagaatatcc agtggactac atcggattta gttattatat gtctacggca atcaatgaga
    14701 ctgatccaca agcagcaact tcagaaggga acttgctggg aggagtaaaa aatccgtttc
    14761 ttgaggcaag tgaatggggc tggcagatcg atccagaagg gctgagaatc gcattgaatg
    14821 aattatacaa tcgttaccag aaaccattat tcattgttga aaacggtctt ggagccattg
    14881 atcatatgga agaggatggc acgattattg atgattatcg gatcgattat ttacgtcgtc
    14941 atatcgaagc gatggctaat gcagtagcag acggtgtaga tttgatgggc tatacgcctt
    15001 ggggatgtat cgatcttgtc agtgcatcaa caggagaaat gagtaaacgt tacggcttta
    15061 tctatgtgga tctagacgat gaaggaaatg ggacactgaa tcgatcaaag aaaaaatctt
    15121 ttgattggta taaagaagta attgcctcaa atggcgtaaa cttgtaatta aagcagctga
    15181 atgaaaaaga acacacttct cacatgtgat ttgtgttctt tttttatttt ttatgtgcta
    15241 ttcttttagt aatagtaaag caaagggtga cgttatgaaa aggacacgtg tactatactt
    15301 ggaagtcgca gataaaatta aagaagatat tttctcagga aaatatccag tcggttcgat
    15361 gttgccgaca gaaagtgaat tagaagaact gtttcaagtc agcaaaatca ctgtccgaaa
    15421 ggcaatcgaa ctgctggcat cagatgaata tgtcgcaaag aaaagtggtc gtgggacgac
    15481 ggttctaagc aatcgtcctt acaatcgatt atccaaagcg gcaagcttta cgcaaatctt
    15541 ggagaactcc cgatatgaag tccggaaaga aacgctggat ttgcaattgg tccatataaa
    15601 gaaggaagat cctttgtttt cctttttcgg tgagcaagct gtttgtttta agcgcttata
    15661 ttatttgaac aatcagcctt atatttattt tgttcattat cttccacaag aaatgatgca
    15721 attcagcaga gaagaatttg gcaaagaatc tttgtatcgt ttgctgacga aaaaaggata
    15781 tactattgac tcattttctg atgatttctc ggcagtattt ttaacagaag aagaacaagg
    15841 aattctgaaa acagacgcac agatcggcat caaacggata cggaaatcgt ctacgcaaat
    15901 tggggaaatg attgaatatt cggaggcggt ctaccatacg gagctgcatc cttaccatat
    15961 tgattatgaa acataaataa aaaaagaggt tgtgaaaaag ctgccctgca tcaagtaata
    16021 agaacagcca aacaaaaata gcttctcata tttttcgttt ggttaggctt attaccgcag
    16081 atacacggct gtttctggat taccatttat tctggataag ggggtgtggc acgattttca
    16141 gaaggaatct atgaaccaat cactgtattg attttagctt cgatggtaac cccattcggc
    16201 ttgacgatcg cttatttctt agggaaaatc attcgtaaaa atatactcaa tcgtcaagaa
    16261 atcgatacat tgaagaccgc ttttcctatg gggatctgtc agatcacaga gggatgtttc
    16321 cctatcgtat tgaacgactt ggcacgcaat gtcatagcaa cgggagtcgg tgggacagta
    16381 ggtggcggat taagcatgtt ttggggcgca gatagccgta ttccggcatc gggaatgttt
    16441 gcagtcgcta cgatgaccag accatgggca ttcattggtg cattgttagc tggttctttt
    16501 gtcacaggaa taaccatttt attattgaaa aaaccagtag atccgaatgc agaaatcatc
    16561 caagaagaaa aagaggaaga agacatatct tgggatgatt tgacaattag ttaggagagg
    16621 cagaatggat tacaagaaaa aagtacaata gatgtacctt gagacaggaa tggtatgaaa
    16681 gaatcagagt tagaaagaat cgaatataca gattttggtt taggagcgat cgaaactgtc
    16741 cagagcatcg gcaccctcct tgaaatgacg atgaaggaaa aatgagaacc tttcgttgtc
    16801 gaaagggtct ggtatacctt atgtcgaagg aaaggaaaca aaaaaacttt cctgtactat
    16861 tccaaaaagg aaagaaagtt attatacagt aaaaaaagaa attattcttc atgctagaga
    16921 tcagtacacg ttcgagccca atatcaaaca ctagttccag gcaggcgcaa caggtgcggt
    16981 tatcactgaa ttttcttcca gcagtgacga ttcaagtgat atttttacaa atccagctat
    17041 ttatcgttaa caaaaagatt tttaggagaa gctgatgaac aaaaatgatc aaattttgat
    17101 cgtttagttc gtcggctttt ttatttagag aaacatcatt tatcagtgaa acataacacg
    17161 agaacaaaat gattttttcg ttaataaaag aaacaagtat ttgttgttta aaataataca
    17221 cgttattggc atataggacg aaatcccagt atcttctata agtaagtcat ttcatatcga
    17281 tgaaaacacg ttatttattt ttgttttatc tataaaatgg ctgaaaaatg atttttttct
    17341 aaatactata tatagtggcg tctatcctat ataccactaa aaaactacaa aatctagtct
    17401 tttattttaa aaaaacacgt gtagaatgaa gatagacttt tgagttaggg agtgtttttt
    17461 tatgaaactt actgtttttt gcggttcccg ttttggtaat aaagaaagtt acaaatttat
    17521 tgcgcaaacg ttaggaaaat acatggcaca agaagaaatc gaactggttt acggtggttc
    17581 atactccggg attatgggag tgattagtaa aacagtactg gaaaataacg gaaaagtcac
    17641 aggtatctat ccgaatgggc tttttgaaac tgaattgcct ggaaaagatg ttactacatt
    17701 tattcctaca gaaacgatcg atgaaagaaa ggtattatta tttgaaaagg gagatgctgt
    17761 cctcgttttc cctggaggtc taggtacatt ggaggaattc tcacaacttc tttcttggat
    17821 agcaatcggt ctgacgcccg acaagccaat cggaatattg aatattggcg gttattacag
    17881 cggcttgcaa caattattag aaacttttgc aaaagaggat tttatggata aaaaatggtt
    17941 ggatcaagta atcttttcca acaatccatt ggaactggtc aatgaattac gagcagtatc
    18001 tattaaaaat cagttattat tgaaggaggc aaaataatga gtcaaatata tttagcagga
    18061 ccattttttt cagaggagca aatcgatcga gtaagtcgta tcgaaaaagc gttggaggaa
    18121 aacaaaacag tcacaagttt ctactctcca cgtcatcacc aagaaagcaa ttatgaacta
    18181 ttcagcgctg gatgggcgca ggaagtatac gaaaaagata tggaagaact gacaaatgca
    18241 gaatttgttg ttgctatcct tgactttgag catcaaacaa tcgatccagg aacggcttac
    18301 gaattaggtg tggcaactat gttgaaaaaa ccgatgatca ttgtacaaga agagacggta
    18361 cctacgaact tgatgattac acaaagtctc catacgtatt taaaatcaga ccaagctgta
    18421 cgtgaatatg attttgaaac attgcctgtt gaaacttacg taggcgagta cttatagaag
    18481 aggaagggtt gtatgtcagt gattttgatt tcaggaacga ttggtgcagg taagagcagt
    18541 ttgacggata tgcttgcaaa agagattgat tctaaaccat tttatgaaaa tgtcgaagat
    18601 aacgaagtat tgccgctctt ttattcaaat cctgagcagt acgctttttt attgcaaatc
    18661 ttctttttga acaagcgatt tttagcaatg aaaaatgctt tagtgaacga tgacaatgtg
    18721 ctagaccgat ccatctatga agatagcctg ttgttccatc taaacgctga tttaggtcgg
    18781 gtaacggata tcgaagtaca gcagtacgat agcttattag atacgatgct aaacgagtta
    18841 gatgatgttg ctccaaaaaa acggcctgat ttgatggtcc atatcaaagt ttctttggac
    18901 acgatgctag aacgtatcaa aaaacgtggg cgtgactatg aacagctaga aagcgatgag
    18961 acgttatata cctattatga aacgttgaac acccgctata atcaatggta tgaagatttt
    19021 gatgtatgtc ctaaaataca agtggatggg gataaattcg attttgtaga aaatccagaa
    19081 gatgcaaaaa aggtgatccg tcagatccaa gaaaaattag ctgaattgga aggaaagcct
    19141 gctggaaaat attttacgga taaccagcat gcaaaaggtc aagaattagg ctaaaaataa
    19201 aaaaccaaac gacgggaaag aaaaattact acgtgatgta gaataagcct ttctatcgtt
    19261 tggttatttt atattcaaat atttgaaata aataaatctt tactctttga tttgtaaact
    19321 gaaatgatcc actgtttgtt ttactgatga agcttgatca aaggaagaag ctgagtttga
    19381 agttcataaa aatcagccat agtgagtttt gcttggattt gtttatcttc ttctactttt
    19441 tacataattt tgaacaggat aagattcaaa tgttttaaat gaatctttga ctagtttgtg
    19501 acagttgtcg catatgctca agcttccttg gtcattaaat tctgtatgga tatttttgtt
    19561 gttattacat gtacaataat caggactatc gatcatccat ttcgtctcct ttacaatcat
    19621 cttgcttctt tactatagca gaaaagaagg ggagaaacaa aagaagatcc aacagacggc
    19681 actatagact gtagatgttg ccatcctcat ccttcaaacg gtaattaccg atttttgtaa
    19741 atatgactgt tgtttctttt ttattttctt ttcctgaaat aatatattcg tcatctttgg
    19801 tagtgatctt gatatctgtc agcactgttt tttcttcaga tgattcagaa gaatcagttc
    19861 cttgtgtctg atacgtgcgt gtccattcca tttttttatc tgtaaatgtc aactgtcccg
    19921 cggataaatc catatcttta cgtactttta gcgtatatac actgttggtg gtatctgctt
    19981 tttccgcttt taagacagaa gaatcggttg tttcgtccga ggattggtca gagccgccaa
    20041 acaaatcaca gccagtcaat gtcaaaagtc ccgcagacaa aataaatagt acgattaatt
    20101 tcttcaaaat acttccccca tttgatagga taaagaaatt ataccattgg aatgtaaaac
    20161 ttcaaacgga tggaagagaa aaacgcacaa aacacccaca aatagagtaa attttagaat
    20221 aaaattttaa aaaggcaaaa aatccgcc
//
LOCUS       DACCWK010000045        20150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCWK010000045 DACCWK010000000
VERSION     DACCWK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.045"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            122..979
                     /locus_tag="IVW65_001540"
     CDS             122..979
                     /locus_tag="IVW65_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7900420.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1094..2014
                     /gene="pstC"
                     /locus_tag="IVW65_001541"
     CDS             1094..2014
                     /gene="pstC"
                     /locus_tag="IVW65_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7900421.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2014..2898
                     /gene="pstA"
                     /locus_tag="IVW65_001542"
     CDS             2014..2898
                     /gene="pstA"
                     /locus_tag="IVW65_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7900422.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2913..3719
                     /gene="pstB"
                     /locus_tag="IVW65_001543"
     CDS             2913..3719
                     /gene="pstB"
                     /locus_tag="IVW65_001543"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7900423.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3735..4493
                     /locus_tag="IVW65_001544"
     CDS             3735..4493
                     /locus_tag="IVW65_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7900424.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4506..5183
                     /gene="phoU"
                     /locus_tag="IVW65_001545"
     CDS             4506..5183
                     /gene="phoU"
                     /locus_tag="IVW65_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7900425.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5421..6451
                     /locus_tag="IVW65_001546"
                     /pseudo
     CDS             5421..6451
                     /locus_tag="IVW65_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            6448..7215
                     /locus_tag="IVW65_001547"
     CDS             6448..7215
                     /locus_tag="IVW65_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7900426.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7315..8883
                     /gene="liaX"
                     /locus_tag="IVW65_001548"
     CDS             7315..8883
                     /gene="liaX"
                     /locus_tag="IVW65_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7900427.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9183..9500
                     /locus_tag="IVW65_001549"
     CDS             9183..9500
                     /locus_tag="IVW65_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7900428.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9500..9850
                     /locus_tag="IVW65_001550"
     CDS             9500..9850
                     /locus_tag="IVW65_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7900429.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10017..10955
                     /locus_tag="IVW65_001551"
     CDS             10017..10955
                     /locus_tag="IVW65_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7900430.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10970..11800
                     /locus_tag="IVW65_001552"
     CDS             10970..11800
                     /locus_tag="IVW65_001552"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7900431.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKLHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11844..12869
                     /locus_tag="IVW65_001553"
     CDS             11844..12869
                     /locus_tag="IVW65_001553"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7900432.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12884..13822
                     /gene="galU"
                     /locus_tag="IVW65_001554"
     CDS             12884..13822
                     /gene="galU"
                     /locus_tag="IVW65_001554"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7900433.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13947..14387
                     /locus_tag="IVW65_001555"
     CDS             13947..14387
                     /locus_tag="IVW65_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7900434.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14390..14992
                     /locus_tag="IVW65_001556"
     CDS             14390..14992
                     /locus_tag="IVW65_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7900435.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15051..16157)
                     /locus_tag="IVW65_001557"
     CDS             complement(15051..16157)
                     /locus_tag="IVW65_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7900436.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16367..17368
                     /gene="ccpA"
                     /locus_tag="IVW65_001558"
     CDS             16367..17368
                     /gene="ccpA"
                     /locus_tag="IVW65_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7900437.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17704..20049)
                     /locus_tag="IVW65_001559"
     CDS             complement(17704..20049)
                     /locus_tag="IVW65_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7900438.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         6405 a         3561 c         4289 g         5895 t
ORIGIN      
        1 aatgagatag ttttttttac aattatgaga atttattttg ttatttaatg attttgattc
       61 gttttcatca agtaatgaaa atgataaaat aaacatactt taggattaaa ggagatttct
      121 aatgaaaaaa cttaaattat tcttaccaat aataggatta gccattcttt tatcaggctg
      181 ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg ctttacagcc
      241 tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat tcatcaatgt
      301 acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg
      361 aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat tagtcgatca
      421 tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg taaaagacat
      481 cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga gtgaagtcgg
      541 tggaaaagat cagccagtag ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac
      601 gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac aagattcaag
      661 cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg ttgctttttc
      721 ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga cagatgaaaa
      781 tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa gagatcatcc
      841 aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac aagataacat
      901 cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg
      961 aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa cttttacatg
     1021 ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa acctgattaa
     1081 gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa
     1141 aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg tgttggttgt
     1201 attatctatt ttttattttg tagctagtaa aggattggcc acctttttta ctgataagat
     1261 caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg gagcaaatgg
     1321 gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga cctttttatc
     1381 tgctatcgtt gccacgccat ttgccattgg tgcagcagta ttcatgacgg agatctctcc
     1441 taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta ttccttctgt
     1501 cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta tttttggcgg
     1561 gacagggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct tgccgactgt
     1621 cacaacgatg actgttgatg cattaaaggc agtgccacgg cactatcggg aagcttcatt
     1681 agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc
     1741 cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag ccttagctat
     1801 ccaaatggtc atcggtaatg cagcattgat gccaacagga cttactactc ctgcttctac
     1861 attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag ttgaaaataa
     1921 tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta atatcgtgat
     1981 ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc agataaaatt
     2041 gctacaggtg ttttatatat agtttcgggg atcatcgtat tgattttagc agctttacta
     2101 ctttatatct tagttcgggg aataccgcat atttcatggt ccttcttgac cgaaccttcc
     2161 aaagcctacc aagaaggcgg tggaatcggg atccagctat ttaactcgat ttatttgctt
     2221 ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta tctttcagaa
     2281 tatgctggaa aaaactggct aacagatgtg attcgcacgt cgattgaaat cttgagctcg
     2341 ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca attccagtat
     2401 ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc tcttttgact
     2461 agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc aggattggcc
     2521 ttaggtttgt cacgctggga aacagtgata aaagtggtgg ttccagaagc gacaccaggg
     2581 attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc tgcattgatc
     2641 tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc attaagtgtt
     2701 tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat ttggaagatc
     2761 aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg
     2821 atcattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg attataccga
     2881 aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg aatgaaacaa
     2941 atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat ctacatgtct
     3001 ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa aataagatca
     3061 ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa
     3121 tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa ggcgtagata
     3181 ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg gtttttcaac
     3241 gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg aaacagcacg
     3301 gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct
     3361 tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct ggcgggcaac
     3421 agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg
     3481 agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca cttgtaaact
     3541 tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagca gctcgtatca
     3601 gcgactatac tgcctttttc tatctaggaa aagtaatgga atatgacaaa actaacaaga
     3661 tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat tttggttaag
     3721 acggaggcct caacatgaca aaagaaatta tttcatctaa agatcttcat ctgtattatg
     3781 gaaagaaaga agcactaaaa gggatcaatc taagtttcaa tcaaggggaa atcactgcga
     3841 tgatcggtcc ttctggttgc gggaaatcca cttatctgcg ttctttgaac cggatgaatg
     3901 acctgattcc gaatgtaacg atcaccggaa gtgttttata taaagacaaa gacatctatg
     3961 gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc cagcagccaa
     4021 acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta aaaggggaaa
     4081 aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg tctgtttggg
     4141 atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggtggg cagcagcagc
     4201 gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg gatgaaccaa
     4261 caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta gaattaagag
     4321 agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt atttcagata
     4381 agacggcatt ctttttagat ggtcacttga tcgaatacaa cgatacgaag aagatattca
     4441 tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga taaggaggaa
     4501 acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat cagttctacg
     4561 aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg
     4621 ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac atggaaacac
     4681 gcttggaaaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca actgatttgc
     4741 gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc gatcatgcgg
     4801 tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg gaaatcgaaa
     4861 aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc ctagtagctt
     4921 atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa agagtcaatg
     4981 actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag
     5041 tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc ggagactatg
     5101 tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact gagttaaata
     5161 cgaaccacaa tgagattttc taaactgaag ctgtgattat gccgtttgtt taggaaaaga
     5221 ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc aagtaataag aacagtcaaa
     5281 caaaaaatag ctcctcatat ttttcgtttg gctggactta ttaccgcaga tgcaagcttt
     5341 tgaacaccgt ttattcggga aagggtttgt gacaaaacat gtcacaaacc cttttcatca
     5401 tttcatagaa aggataaaga atgcaaaaaa aaattagcat catcactata ttttttattt
     5461 gtgtgcttac ttcatgggtt attcagcatc aagctgcttt atatgaagta cctgtcatcg
     5521 agatcgaaaa agtacgaata aaagaggatc atcaattgat tacaggaaga ctgatgaaca
     5581 cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat caaatagaaa
     5641 cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat gaatggcagc
     5701 tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc attggcgcca
     5761 tgttgcttat tggcggaaaa cagggtctat ttgctttagg aagtctcatc atcaatatcg
     5821 ttttacttta cttttgttgc tcatctttac taaaacagaa gggatttctc tattaaatat
     5881 atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttaa atggatggaa
     5941 tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag cattttttat
     6001 ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg aactgaatta
     6061 tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa cagctggagg
     6121 aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta agcgacaacc
     6181 agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag atgtgattgg
     6241 ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat cgcttctttt
     6301 atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat cattggaatt
     6361 tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct cggtcctctt
     6421 ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt tgggatttta
     6481 gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt aggcttattc
     6541 atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg gccgatactg
     6601 gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt tatcaacggt
     6661 tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt ttgtttccta
     6721 ctattgcttc ctatcactga ttttttagcg atccaaggtt ttacatcggt agaactagaa
     6781 gaattatctg gcttagataa gactttagcg attgatttta gattacttac gagatcgttg
     6841 cttctgatta gtcttagtgg tgctgtttta gatgctagtg tagcaatttc tagtgggaca
     6901 ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca ttctagtttc
     6961 gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc attcatggga
     7021 agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca gcaagtgatc
     7081 aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt gtctgcacta
     7141 cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa gaaagaacaa
     7201 ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga atcagtgcat
     7261 aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat ttccatgaaa
     7321 gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga agaaggctta
     7381 gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaataaaaaa agaagcagac
     7441 aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga taaacttgaa
     7501 aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag acaagatcaa
     7561 gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac ttcggcgcgt
     7621 ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga gaaacaggaa
     7681 gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga agaattagca
     7741 cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga tacactgtta
     7801 gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca atggtctgag
     7861 aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga tcaagcaaca
     7921 gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt aggcaaattc
     7981 ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga atggaaagat
     8041 gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt ttactatgaa
     8101 gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg
     8161 tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa aatgggtgct
     8221 gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga tcatatctcc
     8281 ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc taaacgagta
     8341 tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac attggaagct
     8401 aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt aactgcaacg
     8461 atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa catcttagat
     8521 tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga aaacctttct
     8581 gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt gaaaaaagta
     8641 gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat cggtttggaa
     8701 ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta cgaagtagtt
     8761 cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc agatggtgaa
     8821 atcacgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga tacagataaa
     8881 taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt tgtaaaatag
     8941 cttcccctat tttacaaaat tgtgccttat tgccgatagg ctatctcttg aacaccgttt
     9001 accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt tcagaaaaag
     9061 ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa agacaccgat
     9121 taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag aggtgagaca
     9181 tgatgaaaaa aaagttgaca aaatccaata aaaatgttgt tttgacaggg actttagcag
     9241 gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat gtattcctga
     9301 gcttagttgc ttttggaagt ccaatcattt tatacatttt attagctttg attatcccat
     9361 ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa aaaaacaatt
     9421 atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa atcgatgatg
     9481 acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata cgttaacatt
     9541 catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt ggacggccgt
     9601 tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt tgacgatact
     9661 gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca atgcagcgat
     9721 tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt cattcggtgc
     9781 agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg aacataacag
     9841 tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt agaatcagga
     9901 atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg gtagtacttt
     9961 tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta gcatgaatgt
    10021 cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc gtgacaggcg
    10081 acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca ggactagagt
    10141 tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc agtaaagaaa
    10201 tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga agattatgta
    10261 gtgaagatat gcccgcattt attatttcaa gaggattacc ggctccagaa gaaatgatcc
    10321 aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact tctcgtttat
    10381 taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt gtccacggtg
    10441 ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg ataggaaaaa
    10501 gtgaaacagc gttggagttg atcaaacgcg gtcatcgatt gatcgcagac gatcgagtag
    10561 atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc ctggaacatc
    10621 taattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt gcgagtgcgg
    10681 tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca aaagataaga
    10741 aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg ggcattccac
    10801 aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa gtagccgcga
    10861 tgaatttccg tgcacgtaca atgggctttg acgcaacgaa aacatttgaa gaaagattaa
    10921 gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata tgttagcaca
    10981 aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg cattgatcat
    11041 cgtatcaggt atcattatcg ccatgtggct tagcagcaga gaggctgttc gggtaggatt
    11101 aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca ttattggcgc
    11161 tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa tccaaatatt
    11221 caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg cgatcacgct
    11281 gtattttttt actaaatatc attttatttc tttgtggact tttttagata tcgctgcacc
    11341 aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc acgaagcata
    11401 tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat tcatcattga
    11461 taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat cactttggag
    11521 tttgattgga tttattttgc ttttgttttt acgaaagaaa ctacaccttt tgaaaaaagg
    11581 cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta ttgaaggatt
    11641 acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat tatctgctgt
    11701 gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa atttaccgga
    11761 ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc gttaaaaatg
    11821 aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga ccaggctctt
    11881 ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg atttggggac
    11941 atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt tatttaccag
    12001 atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca gtaaaagacg
    12061 cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca aaagaactag
    12121 tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta gaacagggaa
    12181 cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa agaaaagctg
    12241 ttgttgttct ttctggacca agccatgcag aagaagtagc gaaacatgac atcactacga
    12301 tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg ttcatgaata
    12361 attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg gcagctttga
    12421 aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc gataatgcaa
    12481 aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta gcaatgggtg
    12541 ccaacccgct gacctttatc ggattaagcg gagtaggcga tctagtcgtt acttgcacaa
    12601 gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag cccctagaag
    12661 aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa gcagcgatgg
    12721 aactagccca aataatggaa atcgaaatgc ccatcactca aacaatctat gaagtgctgt
    12781 atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat gggaaaaaag
    12841 aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag tcaaaaaagc
    12901 agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag ctatggcaaa
    12961 ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag aagcgttgaa
    13021 atcaggaatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa tcgaagatca
    13081 ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga ccgaattatt
    13141 gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa
    13201 gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac cgtttgtcgt
    13261 tatgctaggc gatgatatca tggaagatga agtaccattg acacagcaat taatcgatga
    13321 ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg atgaaacttc
    13381 aaaatacgga attatcaatc caggagaagt attggaagat gggttataca atgtcaaaaa
    13441 ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta ttggacgtta
    13501 cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag ccggaaatga
    13561 aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat ttgcccgtaa
    13621 attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa caagtattga
    13681 atatggtcta acacatccag aagtaggtgc acctttacgt gagtatatcc tgtctttagg
    13741 taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa taacagaaat
    13801 caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct aattgaataa
    13861 aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat ccttatagaa
    13921 aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga ttgattgcag
    13981 caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa gtttctaaaa
    14041 ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa atcgtcacaa
    14101 aagacgtaga tcttttgtct agacaagtag aaggcttgct ggtcaaaagt aatgaactgc
    14161 tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca gttgctgatt
    14221 taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg aaagtcggcg
    14281 gaatcggtaa agcgacagct aaagcgacgg ttgccggaaa agtcggtggg acagcgatga
    14341 aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa tggctaaaaa
    14401 aggtggattt ttattaggtg cagtagtcgg aggaacagcc gcagcaatcg ccgcgttatt
    14461 gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag cggatgattt
    14521 gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg agttaacagg
    14581 tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag caggtagtgt
    14641 caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg atatcttgtt
    14701 aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg cagaagatat
    14761 gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag atatcatgat
    14821 tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa tgtcagaagc
    14881 aaaaccagtt gcagatgaca caaaagaaat gatgaaaatg gcaaaagaag atgcgaaaga
    14941 agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact aaaatcacaa
    15001 atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc ttatttttct
    15061 aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct tgtcacatat
    15121 acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg ttcaagagag
    15181 aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg gaattcgtgg
    15241 acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta gccatagctg
    15301 ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg acggacggct
    15361 tcttgtgcgg caagctgtgc ttcaagagtg atgttgtata ttttttcttg gaagtcggtt
    15421 ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca aacaacgcca
    15481 agatcgaata acacaagttc attcggtgaa atcttcgtgt ttcctggtgt accatgaggg
    15541 cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga aacaccttgt
    15601 tttttcaatt ggtattcgat ttctgcaatg atcgcttgtt cagtcacgcc ttctttcact
    15661 gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat cagtttgtct
    15721 ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag atcagtgtaa
    15781 gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt caaggcgttc
    15841 ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt tccgatgatc
    15901 tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact actgttttga
    15961 gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag agggataaaa
    16021 agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc gatgtggcca
    16081 gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg tagttcattg
    16141 atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt caataattgt
    16201 accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa attttcatga
    16261 aaacagagat aaaacggttg caattcttga gattatttga tattctattt tagaaatgta
    16321 agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg aaaagcaaac
    16381 aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg tttcacgtgt
    16441 cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg aagttatcga
    16501 tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga aaacaacaac
    16561 agttggggta atcatccctg atgtcagcaa tatgttcttt gcttctttag cacgtggtat
    16621 cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag atggtaacga
    16681 ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg gcgtgatctt
    16741 catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa aaactcctgt
    16801 tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca ttgattatgt
    16861 atcagcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa aaattgcttt
    16921 tgtcagtggt gcgttgatcg atcctatcaa tggacaaaat cgtctgaaag gctataaaga
    16981 agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag cacaatacaa
    17041 cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa cagctgtcta
    17101 tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc gtggcgtgaa
    17161 agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag aagtagctcg
    17221 tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat ctatgcgcct
    17281 attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct tgccttatgg
    17341 aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg ttgtgagcct
    17401 gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatttcat gtttgatgaa
    17461 tattgtgact cgtgattcgg agttggcagg agaacaccgt ttattcggga aaaggggcta
    17521 tgacaaaatt ttgttataat cctttttttt gtcttaaaaa agagagaaag tggttgaaat
    17581 tacatttatc gtatataaaa caatccgtgt atagatgctg cattatgttc accaccatca
    17641 aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt gtattctatt
    17701 aaatcaatca tctttctcat ctgattcttt ggttgcttga ttcgctcttg cataagctga
    17761 agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc
    17821 gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg gtgtttggat
    17881 ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt
    17941 cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc
    18001 ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct
    18061 gtccattccg gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga
    18121 gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc
    18181 ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc
    18241 attgatcaca ctgcgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg
    18301 gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat
    18361 gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg
    18421 aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc
    18481 cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt
    18541 caacacatct tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg
    18601 gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt
    18661 ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc
    18721 catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc
    18781 tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta
    18841 gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc
    18901 atcatctaac atgtatccat aattttctac gccattttgc aatgcagcat agacatcttt
    18961 atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg ccttttgata
    19021 ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg
    19081 tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg
    19141 ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta
    19201 ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc
    19261 aaggccggca gaaagatctt cttttagcgc acctgtgttc gtgtatggac tatacgtgat
    19321 cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc
    19381 tacaccaaag attcctcttg cagcttcctg gactccagca atgttttgtc ccttattatt
    19441 acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt
    19501 ttctacttgt gcagaaagaa tgatctcatt tgctttacgt ttgaatgttg tttcatctgt
    19561 aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc
    19621 agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc
    19681 gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa ttttatcaga
    19741 tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata
    19801 aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg
    19861 caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc
    19921 tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat
    19981 aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt
    20041 gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag
    20101 aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaagg
//
LOCUS       DACCWK010000046        19986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCWK010000046 DACCWK010000000
VERSION     DACCWK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.046"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            69..3458
                     /locus_tag="IVW65_001560"
     CDS             69..3458
                     /locus_tag="IVW65_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7900439.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQEKDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3461..4882
                     /locus_tag="IVW65_001561"
     CDS             3461..4882
                     /locus_tag="IVW65_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7900440.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4879..6756
                     /locus_tag="IVW65_001562"
     CDS             4879..6756
                     /locus_tag="IVW65_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7900441.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6848..7690
                     /locus_tag="IVW65_001563"
     CDS             6848..7690
                     /locus_tag="IVW65_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7900442.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7842..8717)
                     /locus_tag="IVW65_001564"
     CDS             complement(7842..8717)
                     /locus_tag="IVW65_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7900443.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8924..9955
                     /locus_tag="IVW65_001565"
     CDS             8924..9955
                     /locus_tag="IVW65_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7900444.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10044..10349
                     /locus_tag="IVW65_001566"
     CDS             10044..10349
                     /locus_tag="IVW65_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7900445.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10528..12543
                     /locus_tag="IVW65_001567"
     CDS             10528..12543
                     /locus_tag="IVW65_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7900446.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12545..12829
                     /locus_tag="IVW65_001568"
     CDS             12545..12829
                     /locus_tag="IVW65_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7900447.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12855..14228
                     /locus_tag="IVW65_001569"
     CDS             12855..14228
                     /locus_tag="IVW65_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7900448.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14311..16326)
                     /locus_tag="IVW65_001570"
     CDS             complement(14311..16326)
                     /locus_tag="IVW65_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7900449.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16348..17562)
                     /locus_tag="IVW65_001571"
     CDS             complement(16348..17562)
                     /locus_tag="IVW65_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7900450.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17783..18727
                     /locus_tag="IVW65_001572"
     CDS             17783..18727
                     /locus_tag="IVW65_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7900451.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19023..19967
                     /locus_tag="IVW65_001573"
     CDS             19023..19967
                     /locus_tag="IVW65_001573"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7900452.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6810 a         3681 c         3823 g         5672 t
ORIGIN      
        1 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
       61 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
      121 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
      181 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
      241 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
      301 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
      361 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
      421 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
      481 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
      541 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
      601 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
      661 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
      721 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
      781 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
      841 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
      901 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
      961 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     1021 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     1081 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     1141 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     1201 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     1261 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     1321 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     1381 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     1441 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     1501 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     1561 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     1621 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     1681 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     1741 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     1801 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     1861 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     1921 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     1981 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     2041 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     2101 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     2161 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     2221 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     2281 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     2341 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     2401 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     2461 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     2521 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     2581 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     2641 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     2701 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     2761 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     2821 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     2881 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     2941 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     3001 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     3061 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     3121 gacaagaaaa agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     3181 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     3241 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     3301 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     3361 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     3421 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     3481 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     3541 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     3601 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     3661 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     3721 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     3781 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     3841 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     3901 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     3961 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     4021 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     4081 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     4141 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     4201 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     4261 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     4321 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     4381 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     4441 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     4501 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     4561 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     4621 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     4681 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     4741 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     4801 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     4861 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     4921 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     4981 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     5041 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     5101 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     5161 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     5221 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     5281 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     5341 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     5401 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     5461 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     5521 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     5581 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     5641 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     5701 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     5761 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     5821 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     5881 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     5941 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     6001 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     6061 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     6121 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     6181 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     6241 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     6301 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     6361 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     6421 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     6481 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     6541 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     6601 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     6661 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     6721 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     6781 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     6841 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     6901 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     6961 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     7021 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     7081 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     7141 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     7201 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     7261 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     7321 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     7381 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     7441 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     7501 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     7561 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     7621 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     7681 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     7741 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     7801 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     7861 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     7921 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     7981 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     8041 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     8101 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     8161 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     8221 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     8281 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     8341 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
     8401 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
     8461 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
     8521 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
     8581 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
     8641 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
     8701 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
     8761 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
     8821 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
     8881 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
     8941 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
     9001 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
     9061 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
     9121 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
     9181 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
     9241 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
     9301 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
     9361 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
     9421 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
     9481 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
     9541 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
     9601 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aaagggatgt tcgtgaaaca
     9661 aacaaaagat cgacaagaag aacatctgaa aatgttttac atgcctgatc atgaagactt
     9721 tggtatcgat ttgcccgaac attatggtgt attgacctat atagatgaac aagggagtta
     9781 tcatgaagaa aaagtaattt ccgaggttgg tgattacagc cgtgtctacg aagggattta
     9841 cgagacatta gttaatggcg cagaaaaagt catcaaagat gaagaaacaa tcttacaaat
     9901 gaaaattttg gaagaaggaa tcaaaagcat tcaagaatca gaaggaaaaa tatgacatat
     9961 ttgtaagaat cgggtttgtt ttttgacgca taaactgtct tattgtacga tgaaaaagta
    10021 tagaactact aggaggaacc aacatgacaa attatgacgg tgaaaaacac aaacgattaa
    10081 gaggttcaat ggcacctaaa acagaagaag aagtggcttt agcaggaact aattctggtg
    10141 aaacagaaga caaagaagaa tcattagctg gcacaaactc tggtgaaaca gaaaataaag
    10201 aaacagcttt atctggttca aattctgtag aagaggaaac aaagcctaat gagcccaatc
    10261 actttgaaaa agatatcgaa aaagcggttg aagatgtcaa agaaaaattt gaagaactga
    10321 cctcaaaaaa taaacatgat catcattgat cggacgaaat caattcataa aagcaggaga
    10381 catttggtca cctgcttttt ttctgtatcc attcgataga aatcgttcgg tacagtcgta
    10441 aaaaaaatcc aactgtaagt tgtaccaaac acccctcgat ttaaaagcgc taccatacta
    10501 gaatataagt aggaggtgaa cagcgctgtg aactacaaaa ttagttatct cctgaaaaca
    10561 ccaagagaaa tcagtctaga aaaagcagca gagatcatcg aagtaaatac aagcgaggcg
    10621 atcaaacaat taactccttt tgccgaatat gtggatgata cccgtatccg tataaagaaa
    10681 ttccgagcaa tcgactgggc agaaaaattt ttggatatta ctcgatttga agctgtgtat
    10741 accgaacaag agcgaagaga tcttctttat ttgatggttt attcgactgc aacgtctgtt
    10801 tctgtttttc attttcaaga ttttcttcag gtttctaaag ggacagtcct gtcagatatc
    10861 aaaaagataa gaaatgaatt agaagtggaa gattgtcagc tgtcgtatac taggaaaact
    10921 ggttttaggc tgctagggtc agaagcaata ttaagaagag aagcaaaaaa tcgcactgct
    10981 cgtttgatgc agtcacttgc tggaagattt gggctcacgt atttaaccgc aaccctttcc
    11041 attgattatt acgccaaagt tagagatttg ctagataaaa cgatttcact atcagatctt
    11101 cgattcgcac caaatcgaat ggatgaatta gcttattatt tgagtttgac aaccattcga
    11161 attggaaaaa catccatgga actggtaaaa gatgctgaac tgttggcttc tcttgcagtc
    11221 actgacatta gtcgacaagt attgactggc cttggctatt ccgaacatca aaattcggag
    11281 attatatttt tctctctttg cttagcatcg atcgttgaag gagatgtcca ggagcctgca
    11341 cttgattttt tgctggaatg ttcttcagaa gtcatccatc gcatggaatt cctgatggcc
    11401 gtagaatttg cttcgtttcg tgaattattg atgaatgtct tttatcactt ggtccctgcc
    11461 tattttcgta tttcctattc tttttattta cctaatgtga tgatcgatca aattaagcat
    11521 caatatgctt ccatctacga aatgacaaga aaagcattac ttccgttaga aagacgaata
    11581 gggaagagca tccctgaaga agagattggt tttttcacta tactatttgg cggagagatc
    11641 agaaaagcgg acgcagaaga aagaaataga aaaataagag ccgtgatcgt ctgtccaagt
    11701 ggcattagct cttcattgat cttaaaaagt gaacttcagc aattattccc gatgatcttt
    11761 tttacagaga ctaattcagc tgaccgctta cacgaggttg acgaaacttc ttacgatatt
    11821 attttttcta ctgtacctct cacacttact gataagaaaa agtctctcta tgttctgcat
    11881 ccaatcatga ctgacttgga aaaaaatcag ctgatcaatc aagtacaaag agattggctg
    11941 ataccaggat tttcaatgcc ttctgcccaa gaattgttgt cggcgctact gccttatatc
    12001 gaattaaaag aaggcgtaga cgaagaaaag ctctatcaag tattaaatcg taaaatgaac
    12061 aaacttttag aaaagaagga ggatcaacgt cctatgctat ctgaattatt aacagaagaa
    12121 atgatccaat tatcggatca accaaaaaac tggcaagaag caatcacatt tgccgcacag
    12181 ccgctattat cagaaaataa aatagaagaa agttatatca aagctatgat cgagcgcgtg
    12241 gagcagtatg gtgcgtttat ccatattggc gatcacatcg cattacctca tgctcgtcca
    12301 gaagatggcg taaacgaagt agggatgtct ttgctgaaac ttcaacaacc tgtggatctg
    12361 cttgataatc cagcccatcc cattagtttg ttcatctgtt tagctgctat cgataatgag
    12421 gcacatcttc gggctttggc aaatttaaca aagctacttt ctaataaaca aaatcttcaa
    12481 gatttattac acgcatcaac aaaagaagaa atattagcga tcataagaaa aggagacgaa
    12541 tgatatgaaa atttttgcag tatgtcaaag tggattagga acaagtttta tggtagagat
    12601 gaacatcaaa caagcacttg cagatcttgg agcagatgtg gacgagtttg aagtaagtca
    12661 tacggatgtt ggaagtgtat ctagcgatat ggctgattat ttcttcattg agcattcttt
    12721 gaaagatacg ctaacttcta tacctgatga aaagctcgta cctttgcaat cgatcattga
    12781 cagcgaagaa gtaaaagaaa aagtaacgaa gatcttgtca aaagaataaa aatgattcaa
    12841 gggaggaact atcaatggat aaattgttga atttgtttgt gtcaatcgtc agtcagccct
    12901 ccatcctcgt ttctttgatt gcactgcttg gtctcgttct tcagaagaag aaattttcag
    12961 aagtgatcca aggaactatc aaaacatttg tcggattcct agtactgatc ggcggtgctg
    13021 gtttaatcag taattcacta accccttttg cttcgatgtt ccaaacagct ctgcatactc
    13081 aaggagtagt tcctagcaat gaagcagtag tggcgattgc tttgcaacaa tacggtacac
    13141 ctactgcttt gatcatgcta gtagggatga tcgtgaatat tttacttgct agattcacaa
    13201 gatataaatt catttttctt acaggtcaag caatgcttta tgtttcttgt ctaacagcag
    13261 tcattttagt aagcgcgggg tttactgcaa atttgtggat gatcttatta ggcggggtct
    13321 tcgaaggaac actattaact attacgcctg ctctttgtca accgtttatg aagaacatca
    13381 ctggaaacaa tcaagttgcg ttagggcata ccggaaatat cggttatgct tctagtggat
    13441 ggatcggaaa gttttttgga aacaaagacc gttctacaga ggacataaag attcctaaaa
    13501 gtttcagctt tttgcgagat tctactgtat cgatcatgct cttgatgtcg atcgtatatg
    13561 tcgcacttgc tttgttagct ggtcctggat ttgttgaaag agaactaagc aatggagaca
    13621 acgctgtaat ctttgcactg atccaagctg gaacattcac agctgggttt gttatcgtcc
    13681 ttcaaggggt tcgaatggtg ttggcagaaa tcgttccagc attccaaggg attgctaaaa
    13741 aactcgtacc cgattctaaa ccagcattag atgttcctat cgtttttcca tatgcaccta
    13801 atgctgtatt aattggcttt tttgttagct tctttgtagg aacactatcc atgtttgcta
    13861 tgataggctt gcaaactaca gttatcattc ctggggtagt aggacatttc ttctgcggag
    13921 ctgcttctgg tgtcttcggc aactcgactg gcggtagaag aggggcggta ttaggttctg
    13981 ctttcaatag cttattgatt agttggctgc cattatttat ccttccagta ttaggagatc
    14041 ttaaaatcgc ttcttctaca tttgccgata ctgattatct agtaccagga cttgtacttg
    14101 ggaaactagg agaatacggt caaatagcct tgacagttgg aatcatcgct ttctttttac
    14161 ttgtcgtatt atctagcttt ctttttgccg gaaaaagaac aaacattgtt tctaaagaac
    14221 agaattaagc aaacttcaaa ataaaaattg cttggacaat atcagaagaa gatttaaagt
    14281 cttctgatat gtcgcaagca gttttttgtt ttatgcattt atttttgaat agcttgttaa
    14341 gacaagtggc tgttttgcta gaagaggata acttttacca ttcattttca ctgttccgcc
    14401 tgttctatca gaagtaatca cgatcttatc ttttgttttt tcaagcgaaa tctttccatc
    14461 tttgataggt agcgtacaat tcagtgtttc aaatgaatct agattaggtt gtatcataaa
    14521 ttcttcataa cctggctttg ttggatacaa accgataaaa tagcgtccta aaagatagat
    14581 tgggcttgct ccccatgcat ggcataagct ttttccataa ggatctccat acatactgta
    14641 cttttcattt cctgtttgag tagggtcgta ttcttcccag aaagttaccg catcatgaga
    14701 taacatccct ccccaataat caagaatcgt ttggtagacg atattcgtct gtccgagttt
    14761 gcataaagca tcttgttcat agaatttgaa atatggagta gtaatctgtg tgatagtgtc
    14821 attcaataag acattcttta agatcgattg ctgttggtct ttgttagcaa aatcgaataa
    14881 aacagcgaaa atattcgcat gacgcgtcac atttcttctc ccagattcat agcaatcgat
    14941 aaaagcgcca agttcttcat cccaaaagta gtcaaatagg ttttgtttca atagttgata
    15001 tttttcttga tagagatgag tatgataatt ttcgttctct ccatatgttt cagctaagat
    15061 atctccgcaa gtgatcatgg aatgataggc tttcagtaat aagacttgtt ctgcagcaag
    15121 cgttccttct ttatccattt cagaccaatc aataaatatc caatcttttg ctctcccata
    15181 aataaaccct aattcattcg tttgctccat caagtatctc attaatgatt gcatcttagg
    15241 gtagacgatg cgtaaaaatt catagtcttt cgtcatcata tagtggtttt cgataccaat
    15301 caaccacaag atcgaatagt caacgatagt attcatatgc tgcttgattt cattttgtcc
    15361 tcttaatgct aatatcgttc gtttattgat ttcctcgtca aaaaaaagat attgattgat
    15421 catgaatgat tgatacgcat ctccagacca aatccagcga tctcgtttga taccgtcgat
    15481 aaaaaacaaa tcactacata acttgaaggc ttcttctgat actgaccaga tctgattcag
    15541 taaagaagaa tcgcttgaaa aagcagcttg tttcgaaaga gataaaaatt catgaaaaac
    15601 ttgaagttga accattccag tagaaacctc tggtataaac aaatagcgaa aagcgcgttt
    15661 acgagctttc atttttcttg tcattcctat ttgcttatag tagcaataat cgatatccat
    15721 agcttctaca tcggactctc cataacatag agtgatagat tcgatatggt catccagaga
    15781 tattagttct acacagccat tcactgctcg cccaaaatcg aataagattc ctccgccaat
    15841 ctccgtttca gtgactggat ggacgcattc tttttgataa tagatttgat ttggatcttt
    15901 tgcgatatcg acataaagcg gactccagcc agctggatat tcttcaataa aattacttgc
    15961 tgtccagcca atatctgatt ttacttcgtc tccctcgata aacatcgctg gtagccctga
    16021 tgtatgtcct acaaagatac gaatttttgc tttaccagct ggacaagtca gccattttcc
    16081 acatggatgt ttttgtccat taatttctac atacccaact ccttggatcg tcactttaaa
    16141 catggaaggt tgatcaagaa agtaatatct cttaaacttg acgttccgat ggcaatcatc
    16201 catgtaccag tacgctggcc agtcaaaacc tcgttcttcc cgttggaaat tttgtagcag
    16261 tccatggcgg atctcaaagt ctccaggata ccaaagccat ctatctttat gtttttcatt
    16321 tgtcatttta cacgacctcc atcaaattta ttttgttgtc aattgttctt ttatttcttc
    16381 atctactctt atcactgtct ttctattcgt cctgtgtact actagaaggc taataagcgc
    16441 gctaatcaac agcaagatac tgccattgac gaaaacagca agtgttttac tcccaagaga
    16501 agcaaaagag agaaagagca aaccatatgg tgcaatgaaa gtacctaaat tagcgccaac
    16561 caataagatt gaagtattga aactctccgc gtacttgggc gcttggatac ttacttggtt
    16621 gaataagtaa ggaatgaata acgaaaaaga aattcctgtt aggatcgcac caaacccaat
    16681 cacatacagg tttatcacag tggcaatcaa tacaaagccg attcccatca gtagtaaaga
    16741 aagtgtaagc atatagcgat gtgcttttgt ctgaagccag ccaaaacaaa ctccggcagc
    16801 catacttgcc aattgcatga ttgttaagat attcgctgca ttagtttcgt ttcctagaga
    16861 ataagtcgtc aggactgtgg ctaatttaat cgatacagca ttatatacca cgatgaccag
    16921 caagagaaaa ataccataac taatcatttt aggattcgtt ttgattactt gtcttgttgg
    16981 ggctgtgcca tgcgattgaa gttcagttgt ttcaggtgta ggaacaaaca aaacaaacaa
    17041 gacaagaata ggtaagctga tggcgtagac taaaaaggta tattgccaac cgaactgtac
    17101 tagctgtcct gcaacaaatg tcattaaagc tgcaccaaat ccttgaaatg cactttgaaa
    17161 accaatcata gaggctcgtt catctccgtg ataaaaggca gcaatcatgc tgactgccaa
    17221 agaattgatc aatccaaagc caatacctag tccagctctt gaagctaaga caatactgta
    17281 actttgtgag aagaggggga ggataccaca aatcgatgca atcactaatc cgatgatcac
    17341 agtttgtttt tgtccgattt tcctagcaat aaaactacta atcaaaacaa aaatagtgac
    17401 cataaaagag ggagcagtcg ttaataattc aacagcagat aaagaacgcg tggcataagt
    17461 agctgacata ctgggaattg cagacgtgac tgcaccagca gaagtcaaaa taagagagac
    17521 tgacagcaga gaaaatttca tcatcttttt attcataagc atgttccttt catttttgta
    17581 agcgcttatc aaaaatatag tatgaagtgg ttattctctc aatagcggaa tcacaggaag
    17641 ttcatgtccc aaccatagaa agtctttatg aaaccaaaaa tagctttgca tataatagca
    17701 gtacagcgtg aactaataag cgaaagaaac cgcttacaaa aaggagggag tcaattggaa
    17761 ctagctgaat tagacatttt ttttgcgtaa cgaagaatca atcgaaactc tgcagcgtga
    17821 acaaggaatg aatatcaatg atttaaactt agcttaccat cttcagacac cgccaaaaat
    17881 ccaccgaggg gatctatttc aacagggaaa tattcatatc agtaaacacc gtcgatatgc
    17941 ggaagttccc gagcatacac atgattttgt tgaaatcaat tatatgtata gcggtacatg
    18001 tttacaaatt attaatggag aaaggataga gatggaagaa agtgacgtgg tgatcattga
    18061 taaagaagct actcaaagaa tcgaatacac agggcaagag gatattttaa tcaacatctt
    18121 attaaaagaa gaaaccttat ctacagagat attgaatcat ttggcaagca caaataatct
    18181 actttcttct tttttgataa atgcatcaaa agaaaaaggg gaacatacac aattcattca
    18241 ttttcgcggc ggaaaaacaa gtcgtttgac gcatttgatt gaaaatatgc tgttgattta
    18301 ttttgataca tacagtcata agatccgttc gttaaatctc tatacatcac tattactgat
    18361 cgaattgact catttattag aacaacaaag cttttcatca gcagatggat gcgcagaaaa
    18421 tgagatgctt agagtattag attatatcga taagcatttt acttcttgta cgttaaaaga
    18481 aacagcagct cattttggct acaatccggt ctatcttagt aataaattga aaatgtattt
    18541 tgatgcttcc tttcaagaac tggttctaaa gaaaaaattc gactttgcgt tggaactgat
    18601 tcaagaaacg agtttttcgt tggaagaaat cgctaatcgt atcggatata aacaaacgac
    18661 ttctttattc aaattattca agaagtattc aacatgtact cccaatgaat ggagaaataa
    18721 acattaagaa gaaaatcttt cactaaagca tatgaaggaa aattgtttta ggtgcgattg
    18781 ctgtcttatt atatcgttgc agcgaatttt tttggattta ttttcagttc taaacttttg
    18841 gttgtattat caggggatat aggtgtaaac atttgtcatt tatctgttat gcgtattcag
    18901 gaattcgttt ccatcaaaaa ttataataac aactattata attaacatgt tgataaaatg
    18961 aataaacaag actatacttt tcatgaaagt aacaaatagt tatgaaaagg agctgtttat
    19021 tcatgaaaaa aacaagtcgt aaagtagtaa tcgtcggtac aggttttgtt ggaacaagta
    19081 tcgcttatgc aatgatcaac caaggaatct ctaatgaatt agtattgatc gatgtgaatc
    19141 aagaaaaagc agaaggggaa gcattggacc ttttagatgg tatggcttgg ggcgatgaaa
    19201 atgtggctgt atggtcaggt ggctatgaag aatgtaaaga tgctgatatc gttgttatca
    19261 cagctggtat caaccaaaaa cctggtcaat ctcgtttaga tttagttaaa acaaatgcgt
    19321 caatcatgcg ccaaatcgta aaagaaatca tgggatcagg attcgacggt attatcgttg
    19381 ttgcttcaaa cccagtagat attttgacat atatcgcttg gaacgaatct ggtctgccaa
    19441 catcacgtgt tatcggtact ggaacaacat tggatacaac tcgtttccgt aaagaaatcg
    19501 cattgaaatt aaaagttgac ccacgcagcg tccacggcta tatcttaggt gaacatggtg
    19561 attcagaagt tgctgcatgg tcacatacaa ctgtcggtgg taaaccagtg tttgaaattg
    19621 tagaaaaaga ccatcgtatt gcccaagatg aattagatgt gattgctgat aaagtccgta
    19681 atgctgctta tgagatcatt gatcgtaaaa aagcaactta ctatggtatt ggtatgagta
    19741 ctgcacgtat cgtaaaagct atcttgaata acgaacaagc tgtattgcct gtatctgctt
    19801 atttaactgg tgaatacgac gaaaaagata tctttacagg tgtgccatca atcgttgacg
    19861 aaaatggtgt tcgtgaagtt gttgaattat caatcaacga agaagaaaaa gcaatgttca
    19921 gcaaatcaac aagtgcttta agagaagtat tgaacactgt attataagaa acaatgaata
    19981 tgaact
//
LOCUS       DACCWK010000047        19033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCWK010000047 DACCWK010000000
VERSION     DACCWK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.047"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            333..1751
                     /gene="nhaC"
                     /locus_tag="IVW65_001574"
     CDS             333..1751
                     /gene="nhaC"
                     /locus_tag="IVW65_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7900453.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(2255..2698)
                     /locus_tag="IVW65_001575"
     CDS             complement(2255..2698)
                     /locus_tag="IVW65_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7900454.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2916..4256)
                     /locus_tag="IVW65_001576"
     CDS             complement(2916..4256)
                     /locus_tag="IVW65_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7900455.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            4525..5421
                     /locus_tag="IVW65_001577"
     CDS             4525..5421
                     /locus_tag="IVW65_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7900456.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(5571..6512)
                     /locus_tag="IVW65_001578"
     CDS             complement(5571..6512)
                     /locus_tag="IVW65_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7900457.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            6638..7759
                     /locus_tag="IVW65_001579"
     CDS             6638..7759
                     /locus_tag="IVW65_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7900458.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8017..9519
                     /locus_tag="IVW65_001580"
     CDS             8017..9519
                     /locus_tag="IVW65_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7900459.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            9586..10113
                     /locus_tag="IVW65_001581"
     CDS             9586..10113
                     /locus_tag="IVW65_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7900460.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            10324..11385
                     /locus_tag="IVW65_001582"
     CDS             10324..11385
                     /locus_tag="IVW65_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7900461.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            11388..12284
                     /gene="murQ"
                     /locus_tag="IVW65_001583"
     CDS             11388..12284
                     /gene="murQ"
                     /locus_tag="IVW65_001583"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7900462.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            12298..14280
                     /locus_tag="IVW65_001584"
     CDS             12298..14280
                     /locus_tag="IVW65_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7900463.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(14345..14791)
                     /gene="nrdI"
                     /locus_tag="IVW65_001585"
     CDS             complement(14345..14791)
                     /gene="nrdI"
                     /locus_tag="IVW65_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7900464.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            15002..16426
                     /locus_tag="IVW65_001586"
     CDS             15002..16426
                     /locus_tag="IVW65_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7900465.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(16607..17380)
                     /locus_tag="IVW65_001587"
     CDS             complement(16607..17380)
                     /locus_tag="IVW65_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7900466.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(17377..18282)
                     /locus_tag="IVW65_001588"
     CDS             complement(17377..18282)
                     /locus_tag="IVW65_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7900467.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(18436..18756)
                     /locus_tag="IVW65_001589"
     CDS             complement(18436..18756)
                     /locus_tag="IVW65_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7900468.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         6097 a         3455 c         3914 g         5567 t
ORIGIN      
        1 attgacaaat gatcttatgg aaggtaaact tcgtacaagt caacttttca cgcagtagcg
       61 gttaggtgtt ttgttcagaa agtcggtggt cgctgtgaac cgatcaagcc taattgacga
      121 attacatgga aagtccatac atttgaataa aagtttgaaa gtgaaaaaca gatctgtttt
      181 taattaaggt ggtaccgcgg agtcattcgt ccttagttta aaacaggtct ttttattttt
      241 ataaaggagg aatcgcggat gaattgcggc ggagtaatcc gatatcttaa cttttttaga
      301 acataagaac aactaaaaaa ggagagatta tcatgggaaa aaaaggaaca ccaagttttt
      361 tagaagcaac acttgtatta atggttattg ttttaggcat tgcgattggc gttgtcggat
      421 taaaggtctc acctaatatc acgatcttag cagcgatcgg aatggtcatg ttgtatgcag
      481 tgataaaaag atacccgaca gattggctgc acgaaggaat catcaatggc atcaaaccag
      541 gaatcatccc aatctttatt tttattttag tcggttcatt gattgctgta tggatacaag
      601 cagggatcat tccaactcta atggtcattg ggttcaaatt gatcagtgtc cagtggtttg
      661 tcccttctgt atttgttgtg tgtgcaatcg taggtagcgc tgtagggagt gcctttactg
      721 ttatgtcaac gattgggatt gcatttttcg gtattgggac aacactaggc ttaaatccag
      781 cgctggttgt tggagctatt gtttcaggtg ccgtttttgg agataagatg tctccattat
      841 ccgaatcaac aaatctagca gcagctattg ttgatgccga tttgttcaaa cacatcaagc
      901 atatgatgtg gtcaactgtc ccggcatttg ttgtttcttt ctttttattc atgattttag
      961 gacacacaag taaagccgca agtctttcat ctattcgtga agtgactgat gttctagaag
     1021 caaactttac tatttcaatc tggtcactga ttcctttatt tttaatgtta ctgtgtgcat
     1081 ggaaaaaggt gccggcaatt ttaacgattt tgttgaatat cgctgtagca gtaggaatga
     1141 tttttattca aaacccacaa acttccttaa cgaagttggc tggggtcatc gaatcagggt
     1201 ttgtttcaaa tactggaaac caacaaatcg attcactgct tagccgcgga ggaatcgaaa
     1261 gtatgatgcc tactgtgtca ttgattattc taactttgtc attaggtgga ttgttgatcg
     1321 aatttggctt gatctcaacc gtgatggaac gagtatcaag aaagatgacg aacacaccaa
     1381 atttgatttt tacaacattg atgacaagta tcggagtaaa tatttttatt ggtgaacaat
     1441 tcctatctgt tattctgcct ggtaatgcat ttaaagaaac ttatgaaaaa gcaggatttg
     1501 atccaactgt attaggtaga acgctagaag atggtggaac agtaatcaac tacttagtgc
     1561 cttggggaat tgccggaagt ttcgtagcaa gtacatttgg tattcctaca ctcacttact
     1621 tgccattcgt atttttcagt ttgctttctc ctgtgttttc gatggtcagt gccttcacag
     1681 gttggggaat ctcaaaaaaa tttgtgaaac cgcaagaagc agtaagcaag acagcgcaat
     1741 tagaacaata aaaacataaa aagggattgt gacaagactt ttgtcacaat cccttttacg
     1801 aataaacggt attcaaaagc cagtttctcg gtattaagcc aaatttatac aaaaatgtgg
     1861 aaagccattt cacaaaattt ttcttaatac gcgaaactaa gcgacttttt cacaacctct
     1921 cgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg caaaatatga
     1981 ggaactattt ttccatttgt ctctctaata ctcaggtcag aacgctgatt ttacaacctc
     2041 ttaataaacg gtgttcaaaa gcttgcatct gcggtaataa gcctaaccaa acgaaaaata
     2101 ggagaagcta tttttgtttg gctgttctta ttacttgatg cagggcagct ttttcacaac
     2161 cccttattca cggcgttcca gaagtagctt cttcttattt cttgaagctg ataacttctg
     2221 gaacatcctc tttttttatt tggacggata atggtcattc catttctttg atcatttttt
     2281 tcattttgtc gatctcttct tcttgtgcct gaatgatttc ttccgctaat ttttttactt
     2341 caggatcttt taaatcagct cgtttacttg ccaggatagc gatagaatga tgaggaatca
     2401 tggccttcat ccaagctgtg tcgttgacag tagtttggct tcgtaccata aacaaggaac
     2461 cggcaaaaac aagaagacta gcggctaaga tcataacatt tatctttttg ttttcataca
     2521 ttttccacat gaacaacagc atgatgactg ccatgactgc acccatcatc aacgccatga
     2581 atactcgtgt acgactgaag aagatatgat cgagcgcata gacattcaag tacatcatac
     2641 caaacataac taccgttgaa acaccgatca tcaacaaaaa cttccaatac tttttcattt
     2701 aaaacaaact cctttttttc attcctttct tctatcatgt catgagcatg gagaacaatc
     2761 aattattttg cacagagggt ttttgaacac cgtttaccaa gaggtcatga gactgtttat
     2821 tcggaaaaag gagttgcaac aagacttttg tcacaactcc tttcttcaat agctccatct
     2881 attccacata aataaaataa aattttctat cgatttcatt cattcgtcaa tacgaacgca
     2941 tcattctttt tttgataact gaaataaatg aacaggtaaa gcaggccagt caatacagtt
     3001 accccaatgg agttcgtgaa caggatacca aggaaaatca atcctaagac agctaatgca
     3061 aaaaagctga ttcctttatc ccataatgtt ccttttctta gtgccagtac aaaggatgcc
     3121 gcactgataa tgatccagat caataatact gtatacgaaa gggaacctgc cagatagcca
     3181 aataatttgt caccgacaaa ataagaaaga gctacaccaa gataaagaac actggtacaa
     3241 aataaaacag cacgttgcgg tacttgatgc ttgtttaaaa cagctagttt tctagaggaa
     3301 ccttttcgat ccttcaaacg gaagaacaaa attcttgaag atgcatacac acccgaattg
     3361 atagatgaga acaaagcaag gacaatcaca aaattgacga tatccgctgc aaatgggata
     3421 ttcatttttt caaataccat cacaaaagga ctgacattcg tgcctgctaa agtattccaa
     3481 ggaaaaagaa tcaaaagcaa aaacaaagga atgatgtaaa aggaaatgat tcttcccatg
     3541 acacctttga ttgctttagg aatcgcacgt ttaggatcat ctgcttcgct taccgtgata
     3601 gcaatcagtt cagaaccgcc gtaagaatag ataactacaa gcaaagaact gatgattccc
     3661 ttcataccat gaggagtaaa cccaccatgg tcagtcaatt gctgaagggt agggaaaacg
     3721 cctgtgccca acatttgctg accgacaaga taagtagcaa aaatgatcag taagataatg
     3781 acactgattt tagcaaaagc cagccaatat tctgtttctg cgaataaccg taccgaataa
     3841 agattgatga tcgtagttaa aacagccaac ataaaaacaa atacccaggc tggaatgtgc
     3901 gggaaccaca actgcaaaaa ggaagcagct gcaacagctt cagcgatgat attgataatc
     3961 cacatagacc agtagaccca atcagtaaaa tcagcagaat gagcacctag ataagggcgt
     4021 atcaaaccag ataatccgtg atgcgtgtcg ccaccgctta aaacgatttt tcccagacct
     4081 ttcatcacga tatataaaat cagcccacca atcaaaaaag caatcaatac agaagggccg
     4141 gcgatcccaa tcgcttcgcc acttccttta aatagacctg caccgattgc tccacctaaa
     4201 gctaacatgg tgatatgtct ggagctaagt tgttttttca attttttatc ttccataaat
     4261 gacctctttc ttttaaattc acaatcaaaa tttatattaa gaataaatag caatatttaa
     4321 caatgactaa tgataacaaa ttttcagaca attacaacta ttcagaaaac ttttaggcga
     4381 attttaatag aagaatgatt tatttcatca tattttcata caaatgttaa attttcatac
     4441 aaatatagga aacgttttac tggcttttta ctatttaaac aggctatcat aggaaaaagt
     4501 gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg tactgctagt tgcaaaaaat
     4561 aaatcacttt attttttgcc ttccatccgc tctttagatg tttggtccgg aaaagattta
     4621 ctacagtcgg aattcattaa atcagctaga caacagttgg caaattatca gctgatcgtt
     4681 tttttagatt atggatttga tatggagatg gctcgacgcg tgcgcccatt taccaatgca
     4741 aaaattgtgt tatttttttg gaatcacttc aaagcagaac acgaggcgat gctgaagcag
     4801 gcacaagatg aaccggcaat cgatgagatt tatcattttg attttttgga agcaaaagaa
     4861 cttgggctga aacataatag ttcattttat tcgaaacata tgaatttttc ttctagcgaa
     4921 ccaaagacgg atctattttt tggtgcaacg aataatggga gaaaagaacg agcagaaagc
     4981 tataagcagg aatttataaa aagagatgtt acgaccaact atttcattct tccacatcga
     5041 gggaatgaac aacctggata tcttacttat gaagagtacc tgaaaaaaac aggggaatcg
     5101 cgcgggattc tggaactgtt gcgggagggt cagcaaggag tgactttgcg gacatttgaa
     5161 tccatgtatt ttcaaaagaa actagtgacg gataatcaag cgattgctca ttatcatttt
     5221 tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt tagatgaatt accggaattt
     5281 ctgcatacac catttaagcc aatcctatca gagctattgg aattttatga tgcagaaaat
     5341 tgggcaaaac gatttatcac taacgattcc actatttatg agcaatacga atatgacctc
     5401 tcctctgttg aaaacaaata aaaaacctac attttgaaga gattatgtac tgaaacaaac
     5461 aagcacaatt cttatggcat catgagagtt gtgttttctt tttgatcttt tattgggctt
     5521 aaaaaaagag aaccagctgc tgattcagct gattctcatc agagagcctt ttattctgcg
     5581 ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata tggtgtagca
     5641 atatcatatt tttgtccttt tcgccaaatg gcaccattga tgtagtcgat ttcagtcaaa
     5701 cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat gttgtcagga
     5761 tcataagtag aagcaatatg ttggtacact tcttcttgat ctagtatgat tccttctttt
     5821 gctgcgatag aagcaaattc agatacgatt gttcgaacca tgctctctgc cgctttttgc
     5881 gcgcctagtt cagcaatatt acaatccaaa atcgtgcata aaccattcaa tgttccattt
     5941 acacaagctt ttcgccaaat ggaataacga acgttatggc tatagatcgg atttaaattt
     6001 gcttcttgaa agacttctac aacttttttt gtaaatgctt cgccttctgg ttccaagttc
     6061 tctagttcga tttctccatc acctaacaat gtgacttttc cgggaccagc tagaccagct
     6121 gtccacatcg tgatcccaaa taagatattt ttctttggca catatttttc cagtacatct
     6181 tcatgaccta atccatttag taaacacaaa acataagtat tgtcagtaat aatggattgg
     6241 atcgaacaaa acatatcatc taattgattg gcttttgtta atgctacgat caagtccacg
     6301 tgttcgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc gaccacttct
     6361 ttaccgttaa agtcagcaat caacccattt gtacgaatgg cttcaatgtg agcaggccaa
     6421 cgatcgatca agaggacctc atttccgctt tgatgtagca tcaatccgat tcggctgccc
     6481 attgctccgg ccccagctat tgcgattttc atgttttttc tctcctttat tcaatgagtt
     6541 cccttttagt atatagaaaa atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa
     6601 aaaagtgtct tacttaagaa gaaagtgagg gatacagatg gatattttgg cggcaattga
     6661 ttcatttaaa ggaagtgctt cttcatttga actaaatcag gcagttctaa atggctggga
     6721 agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga
     6781 agcaatctat tacgcgctca ggggaacatg gcgtaccatt gatgggatgg atctcttatt
     6841 ccgtcagcga aaaatcagct atttattgac aacttggcaa ggaaaaaaca ccgcagtcat
     6901 cgaatctgca caagcattgg ggttagatct acttgataaa ccaacggatc aaactatccg
     6961 acttgcttcc agttatgcct taggagaact tattaaagat gcactgcttc aagaagtaga
     7021 acaaattatt gtcactttag gaggaagcgg gtgtagcgat ggtggtctag gattactcca
     7081 atccttgggt gcttctttag aagggtacac agaaggaaat cctttattat ctactaaaaa
     7141 acttcatttt gatcgaaaaa acgttcttgt ttctaaagta aaattgatta tcgctgcaga
     7201 tgttaccaat ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg
     7261 aggaatagat acgacgctag ctttcttaga taaacaagca ggaaagattg ctcagcaaat
     7321 aaaagatttc tgggaggtgg atttagatga tattccagga agtggagcag caggcggtat
     7381 tggtggagcc ctttttttgt tgggagcgaa gatggtttct ggtttcgatt tagtcagcca
     7441 aatcactgga ttggaaaaaa agatcgaaca agcagatgtc atcattacag gagaagggcg
     7501 gatcgatcag caaacgatcc acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa
     7561 atatcgaaaa ccaataatcg ctatttgcgg aagtagagac caagagctag aaggactttc
     7621 tgattttctc ccaattgtct taagtattca tttgggtcca ataggtttag aagaagcaat
     7681 ggaacacagg caaacattag aaaaaacaaa aatcttagga cagacactta gcagattttt
     7741 caccatcctc ccgaagtagt tgacaaaatc attttcttta tgctatcgta atttcaaata
     7801 taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc
     7861 agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt
     7921 ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga
     7981 aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat
     8041 ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt
     8101 gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg
     8161 aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc
     8221 tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt
     8281 acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa
     8341 atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca
     8401 cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat
     8461 atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg
     8521 gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa
     8581 agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt
     8641 gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa
     8701 acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa
     8761 gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct
     8821 gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag
     8881 aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg
     8941 tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta
     9001 ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac
     9061 ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg caaggttata
     9121 ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac
     9181 aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg
     9241 aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg
     9301 agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta
     9361 tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa
     9421 ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat
     9481 aaagtatcta ctgagattat ttctcttgag agaatctaac ccttgtaatc tcaagaacaa
     9541 caaggtatgg ttaaggggaa ctttgccaac gaaggagaga atcacatgct ttatgtaacg
     9601 agacacggag aaacaacttg gaacgcacaa ggattagttt gcggacgggc agatgtgccg
     9661 ctgactgaaa aaggacagat gcaggcacag aaactagcag aaaaggtagt agatttgcct
     9721 gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca
     9781 gttgctgatc gtctcagctt gccgctgacc gttgatgaac gattggtaga aatggatttt
     9841 ggggattatg atggactgcc aagcaaagat gagaactttc aaaaagcacg tttagcgttc
     9901 gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat cgttccttta
     9961 ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca taatgccttg
    10021 ataagagtga tcaatgctta ttttcatccg atgcctaatg aggggttctt tacattcatg
    10081 gtagataata cagaattggt ttcttatgag taattatgtt tttgtgttat ttaaataaaa
    10141 tatttcaagg cactgctttt gcttagcagt gcctttttta ggttatgaca attcttttga
    10201 taatataaac tcaaccttta gaaatgaaat ttaattattt taataaaagt attgaaattt
    10261 tgtttcttta gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat
    10321 aatatgtttg gcttttcaat atttatgaac gattctttgt cagaagagaa gaaacagtat
    10381 atccgccaaa tggcatccaa tggctttgtc ggtattttca cttccatgca tataccagaa
    10441 gatgatgttt cggcatatca aaagcgattg atcgaactag gaagcgaagc gaaaaagaat
    10501 aaactagaat tgatggtcga tatttcagga gaggcattgg aacgagcggg tttttcatta
    10561 gaagatctaa cgcctttgaa agcaattggt gtaacaggtt taagaatgga ttatcatatt
    10621 tccaatcagc agattgccga ctggtcacat caattgaaaa tcagtttgaa tgctagcacg
    10681 atcacgccta aagatatcga tgaattgaaa gaagcagaag ctgatttctc acagattgaa
    10741 gcttggcata attattatcc acgtcccgaa acagggctgg acaaagaatg gtatcagaaa
    10801 aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc aggagataca
    10861 gagttgagag gcccactata tcaaggattg ccaactttgg aagaacatcg agggatccat
    10921 cctcttgcag gcgctctaga tctattagca agtaacacgg atattgttta catcggagat
    10981 gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc
    11041 ttgctccata cggaacctgt agatgaagaa ttttacgaat atatattagg gaagcatacg
    11101 aatcggcaag acgatgcaag agatgtcata cgaagcgtag atgcacgttt tcgagagatt
    11161 ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag ggtctattac tttagataac
    11221 gaaaagtatt tgcgctatat gggtgagatc caattaacca aatatgattt gccagcggat
    11281 gaaaaagtga atgtagtagc taaagttatc aaagaagagc taccattgat caaccaaatc
    11341 aaagctggta tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca
    11401 atttagagaa cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgagatga
    11461 gtatttctca agccgttcaa ctaatgaaca aagaagatca gaaagtggcc gctgcggttg
    11521 aaaaagaact gacaagtatc gaaccagtga ttgctaacac gattgagtcc tttaaaaaag
    11581 gtggacgtct gatttatatg ggagccggaa caagtggaag actaggtgtg ttagatgctg
    11641 ctgaatgcgt accgacattc ggtgtagaac cggaaatggt cataggtcta attgctggtg
    11701 gagaagaagc aatgactgta gctgtcgaag gggccgaaga ttcttatgag ctagggcaag
    11761 aagatctaat caagctgaat ttgacaaaaa atgatatagt tattggtatc gcagctagcg
    11821 gtcgtacacc atatgtgatc ggtggattag attacgcaaa atcgatcggt gctgtcacag
    11881 gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc ccaatcgaag
    11941 tcgatgcagg tccagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa
    12001 aacttatctt gaatatgatt tctactatca gtatgattgg tataggtaaa gtattcaata
    12061 acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca aaacgaatca
    12121 tcatgcaagc gacagaagct gattacgaaa cagctgaaaa gtactttgaa gaagcagatc
    12181 aaaatgttaa gctggctatc gtcatgattt tgacaaatag tgataaagag gaagctcaag
    12241 caaaattagc tgaagcaaac ggatttgtaa aagggacctt atgaggggga aaagaccatg
    12301 gcaaaaaacg aactgacttt ggaagaactg gcaaaacaaa tttataaagg ggcaggcggc
    12361 atgggcaatg tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa gatcatcaat
    12421 gatgatcttg taaatgtctc tgaattaaaa gcgattccag gagtgcttgg tgtagtagaa
    12481 gatgaacagc ttcaagtcat catcggacct gggaaagtga acaaagtagc acagcaaatg
    12541 gttgatttgg caggcgtgaa attaggagaa aaactttctt ctgtaagtac agctacagca
    12601 aattcatcag catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca
    12661 caaaaatcga aacaaaaacc atctaaaatc aaatcggtat taaaagacat ttctaatatc
    12721 tttgtgccaa tgattcctgc atttgtcggt actgggatcg tggcagggat tgccgcagtt
    12781 ttagcaaact tagtcacagc cggaacactt gatgcagcaa catggcaaca atatatcgat
    12841 gtgatgaaca tcttgaaaaa cgggatgttt agctacctag ttatctatac aggtatcaat
    12901 gctgcacaag tttttggtgc aacaccaact cttggtggag ttatcggggc cgttgtgatg
    12961 ctgacaggga tgaatccgga agcaccactg aaaaacttat ttactggtga agctcttgca
    13021 gctggacaag gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc aatcgttgag
    13081 aaaaaactgc ataaagtagt acctgatgcg gtagatatca tcgttacacc aactctttca
    13141 ttgattgcca tcggtctgat tgaaattttc ttcatcatgc ctttagcagg tttcatttct
    13201 gatggcatgg tcggtgggat caactgggta cttggcgttg gtggagcatt tgccggcttc
    13261 gttctaggca cattattctt gccgatggtt atgttcggac tgcatcaaat tttgactcca
    13321 atccatgtgc aaatgattga tgaaactggt cgtacgctat tgcttccaat tcttgcgatg
    13381 gctggtgcag gacaagtggg tgcagcattg gcattatgga ttcgctgcaa aaaagataaa
    13441 gaattaacag aaatgatcaa aggagcttta ccagtcggga tcctaggaat cggcgaacca
    13501 ttgatttacg gagtaacatt acctctagga cgcccattta ttactgcttg tatcggtgga
    13561 ggtatcggcg gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc tattggacct
    13621 tcaggagccg ctttgatccc attgatctct gacggtaaat ggttaggcta catccttgga
    13681 ttattggcag cttatgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac
    13741 cgtttagaaa aagaagaatt agcgactgaa acagccacag taaatgaagt gcctgaaaaa
    13801 gtagtacctc aaacttcagc tgaaagtgtg actatcctct catcagttgc tgacggaaca
    13861 gttgtttctt tagataaagc aagtgatcca gtttttgcac aaaagatgat gggtgaaggc
    13921 tactttgtag aaccagaaaa cggacatatc tattctccag ttactggggt agtcagctct
    13981 gtcttcccaa cgaaacacgc tattgggatc acaacgccaa atggtttaga aatcttgctt
    14041 catatgggaa tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa agtaacagaa
    14101 gggcagcaag tgacacctga tacgcttgta gcagaagctg acttagaagc gatcaaagca
    14161 gcaggcaaag aaacatccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt
    14221 gtcttggaaa aaacaggtaa agcaaaagca aaagcacctg tgatggatgt caaagcttaa
    14281 tatgatagaa acaatagaaa cgaaagaaga aactgaaatc tatttttcag cttcttcttt
    14341 tttattattt attgttttct gctaaaatct gataaattcg tgtcacatca gatggtgttc
    14401 cccgtaattc ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat tgtttggctg
    14461 ttagacaata ctgattattg aagtttttat ttccgctacc gactacacca agacaaagtt
    14521 tatgattgtc ttcatgttcc agatattcac gcatcgtttc ggtcaagatt tctgtatcac
    14581 cgttatctaa accatttccg ccgtctaaat aagtaggaac gaaagtaaaa aatggttctg
    14641 tttctttcgt aaaatcacta ttttcatgga tttcttttaa agtaacttca ggattgacag
    14701 gatcttctgc atgcattttt tcagcatatt ctgctagatg tttggcaaag gcacgcgtat
    14761 ttcctgaaat cgatatatat aaaatgttca tggatcatcc tccaattctt tccttttagt
    14821 atagcatctt ttttaagttt tttggtggtg taatttggaa agtgactagg cgctttgctt
    14881 gtataatgag aaaaaagccg gtagaattat atttctatta aagtttttat taattaagta
    14941 aaaataaaaa aggtttgtta tactttttga taaaagcggt tttattttgg aggatgctta
    15001 catggataca gagcaacaga aaaaaggaat tggtttaatt ccacttgctg ccttagtaat
    15061 tggttcagcg attggaggtg gcgtatttgg tattatgacg gacatctccg gatcggctgg
    15121 cggcccagct attttcagtt gggtactcgt aggaacagca atgttgatgc tctccttgtc
    15181 gatcaataat atcaataaaa aacgtccaga tttaaaaggc gggatcttta gttacgcaga
    15241 agaaggtttt ggccgtttcg ctggttttat cagcggttgg ggttactggc tcacatgctg
    15301 gttaggcaac gtagcttttg caacattgtt gatgagcgca ttaggatatt tcttcccagt
    15361 atttgaaggt gggcaaaatc tggcatcgat cgtgatgagt acagtctttt tatggggata
    15421 tgcatggcta gtgaatcggg gagtcgaaaa tgctagtttc gtcaatgcta tcgtcacatt
    15481 ctgtaaatta gtgccattat ttttatttat tgtagtagct atcagcacgt tttcgatcca
    15541 acaattcatt gataactttt ctagcgacat tatattgaat gcgaatggac agccgatccc
    15601 gttttccagt caagtattga gttgcatcat ggtgatgctt tgggtatttg ttggtattga
    15661 aggagcctct gtcgtaggtt cacgagcaaa aaaacaatca gatgttggga aagcaacgat
    15721 tctagggata ttcggcttga ttattattta cgtcttggtt tccatgcttc catatgggac
    15781 aatgacgatg aatcagctgc aaacgatcaa ggcgcagcct gctatgggct atgtgtttga
    15841 aatgatggtt ggtaaatggg gagcagtcgt gatcaacggc ggtttgatca tttctttagt
    15901 tggtgtttgg ctctcatgga cgatcttacc gattgaaaca atgggaaaca tggctgaaga
    15961 cggacttttg cctaaagcat ggggaaaact caacaaaaat ggggcaccaa cttatgctat
    16021 cgtattgacg acgttatgta cgaatgtctt tttactttca ttattattca ctgacagtgc
    16081 ttataacttt gcttacacac ttggtacagc agctattttc ttcacgtggc ttttcttagg
    16141 attgtaccaa atgaaacttt cttatcagcg gaaagaatgg gtccaatttt tagtagggct
    16201 tttagcaagt gcctttcaag tatgggctat gctagtcgta gcctttaatg aagtaatgct
    16261 tgttcttgtt cttttcttgc caggcattta tttttacctc aaagcacgta aggagcaaaa
    16321 cgagcctgta caacatatcc acttagataa gattttactt ataggtgtga cgatggtcgg
    16381 agtgattgca atggttttat ttgccactgg gatgcttcac gtatagaagt ataaaagaag
    16441 tataaagaga ggttgtgagc ctgtcgttta ttcaaaaaca tactaggaaa ggagctgtga
    16501 cacaaatgtt gtcacagctc ctttttcaga ataaacggtg tttaagagac agccaatcgg
    16561 caataaggct gaacgtctct ttcacagcct cttttttgat gagtcatcat acattttttc
    16621 gcttaaatac gagatatcct aatgctaaga acaaaacgat gtaagctaaa ttaccgaata
    16681 gcatttgatt agtagaaaga tgagtcaatt gcttcatgat ctcttcgttt ccaatttggt
    16741 tttgcagatt caacatactg atcggattcc atttgatcca ctcccatttt tggatgagtg
    16801 caataaggat accagatatg attgacgaag caaaataaaa gacgattcct gctgagatgg
    16861 atgcgccgga actattgata aagcaagcaa tcatcaacac taaacttaaa atcagccata
    16921 aaccgatata actacccaaa gtataagtga acaagctttg gatcagtgtt tgaccagtag
    16981 aaaccttctc cgtgaaggaa atcgaaggga aaagaatcag tttcatcaac acagtgacaa
    17041 taatagctaa aaggtataaa taaacagaat aaataactaa tgtgatccat ttgcttacta
    17101 atatttctcc tcgagaatac tttcgataaa ggagattttt caatgtacca tattgtgctt
    17161 ccatcgaaat gatcgtgctt gcagctgcaa tcatgataaa gacaatccaa gatgtggcac
    17221 taaaaagctg agcagtcatt tcaactggat caatgatcgt cgtgtatttt ttggctagaa
    17281 gtgctgttcc tatgagcaaa acaactaata gaacagataa aacagcagtt gactttttct
    17341 tgatcaattt aaataattct tgttttacaa gcaatgtcat ttttcttctt ccccctttac
    17401 tgaatccaga atttccaata gagaagattc caaatcattc gtcagatgct ggacgtctgt
    17461 cacatggata ttttgtgcag acaatagctg gatcacggtt cctaataatt cttcgttttc
    17521 ttttaaaagc ttgattttat caggatcttc caaagtatat ccagcttctg tcaagatcgt
    17581 tcgagcagct agatcatttg ttgtagagat aacgatgaaa tcttgcgctg tttggttcaa
    17641 ttcagccatt gtacattgtt tgatgatctt tccttgattg atcaggatca gctcatcgac
    17701 cattttttca agctcgctga gtaagtgact ggagatcaag aaagaggtgc cctgagtagc
    17761 caagtcggta atgatttttc gcacatctct tgttgcttgt ggatccaaac cgttcatcgg
    17821 ctcatctaaa ataacgatag acggatggtt cagtaaggca agagcaattc ccaatttttg
    17881 tttcattccc aacgagtagg atttcgcttt tttgtgaatg tacttttcca tcttgagctg
    17941 attcacgata gtgtcgatag cagatacatc gttggtttca gagaataact tcagatgatc
    18001 atagcctgtt aagaatggat agatacccgg gtattcgatc aatgctccta cttgttccaa
    18061 ccctttatga ctagagggag aaatctcatt ttcaccaata cagattgttc catctgagta
    18121 atggatcagt cctaaaatag ctttcatgat cgttgttttt ccagcgccat taggacctac
    18181 taatccaacg attttgccag gggataagct aaaactgaca tcgctgaggg cttgtttgtg
    18241 accaaagttc ttggataatt cttggaccga aagacttttc aaaaatattc actccttttc
    18301 tatagatagt acaagcatac acctgatgag tggagaagtc gctaggtcat tggacttatt
    18361 tcttattttt tcataaaaaa attgaatatt aaataaaaag gcttattttt tctgatcagt
    18421 acttattcca agtgtctagt ggccgctagt aatcttgatg ccaatgatac tgatgagtag
    18481 catccccaca aagaaccaag tggaaagatt cagcttatca tgaaacaaga ccacaccaat
    18541 tatgaccgaa ccaactgcgc caatccctgt ccagatagga taagagagac tcatcggcaa
    18601 acttttgatt gcctttgaca aaaagtaaaa actagcaatc atgccaatca ctgtgtaaaa
    18661 agaataattg attttagaaa aaccatcact ccatttcatc atcgttgacc agaatacttc
    18721 caaaattcct gctatgatta attccatcca tgccatactt acattcctcc ttaactataa
    18781 aaaaatacac aaaaagagga aaatcatccc ttcaaagacc taatggaatg atcatcctct
    18841 ttatcctacc cggtggcaga ttgtatatct ttttctgtag tatagcacac ctatccagat
    18901 atcggcaatc tcatactttt tagtaaagtg taccaagcac tgacggatat atcgttagcg
    18961 gaaaagaaga tgaatgcttt gcaatttcat agaaagaagt tgttatttat taatcattta
    19021 aaaaaaacag taa
//
LOCUS       DACCWK010000048        18564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCWK010000048 DACCWK010000000
VERSION     DACCWK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.048"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            6..305
                     /locus_tag="IVW65_001590"
     CDS             6..305
                     /locus_tag="IVW65_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7900469.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVW65_001591"
     CDS             378..1739
                     /locus_tag="IVW65_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7900470.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVW65_001592"
     CDS             1761..2021
                     /locus_tag="IVW65_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900471.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVW65_001593"
     CDS             2035..2787
                     /locus_tag="IVW65_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7900472.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVW65_001594"
     CDS             complement(2881..3486)
                     /locus_tag="IVW65_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7900473.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVW65_001595"
     CDS             complement(3539..4513)
                     /locus_tag="IVW65_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7900474.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVW65_001596"
     CDS             complement(4891..5670)
                     /locus_tag="IVW65_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7900475.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVW65_001597"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVW65_001597"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7900476.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVW65_001598"
     CDS             7161..8558
                     /locus_tag="IVW65_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7900477.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVW65_001599"
     CDS             8639..9079
                     /locus_tag="IVW65_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7900478.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVW65_001600"
     CDS             9227..9553
                     /locus_tag="IVW65_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7900479.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVW65_001601"
     CDS             9554..10303
                     /locus_tag="IVW65_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900480.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVW65_001602"
     CDS             complement(10383..10838)
                     /locus_tag="IVW65_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7900481.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVW65_001603"
     CDS             complement(11265..11540)
                     /locus_tag="IVW65_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900482.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVW65_001604"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVW65_001604"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7900483.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVADPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IVW65_001605"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IVW65_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7900484.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IVW65_001606"
     CDS             14770..17436
                     /locus_tag="IVW65_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7900485.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IVW65_001607"
     CDS             17800..18552
                     /locus_tag="IVW65_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7900486.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5966 a         3213 c         3831 g         5554 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgc ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct tttt
//
LOCUS       DACCWK010000049        18149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCWK010000049 DACCWK010000000
VERSION     DACCWK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.049"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(61..1539)
                     /locus_tag="IVW65_001608"
     CDS             complement(61..1539)
                     /locus_tag="IVW65_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7900487.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1837..2772)
                     /gene="murB"
                     /locus_tag="IVW65_001609"
     CDS             complement(1837..2772)
                     /gene="murB"
                     /locus_tag="IVW65_001609"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7900488.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2845..3765)
                     /locus_tag="IVW65_001610"
     CDS             complement(2845..3765)
                     /locus_tag="IVW65_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7900489.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLPQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3878..4630
                     /gene="xth"
                     /locus_tag="IVW65_001611"
     CDS             3878..4630
                     /gene="xth"
                     /locus_tag="IVW65_001611"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7900490.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5494..6027
                     /locus_tag="IVW65_001612"
     CDS             5494..6027
                     /locus_tag="IVW65_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7900491.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6517..7041)
                     /locus_tag="IVW65_001613"
     CDS             complement(6517..7041)
                     /locus_tag="IVW65_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7900492.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7034..7507)
                     /gene="tsaE"
                     /locus_tag="IVW65_001614"
     CDS             complement(7034..7507)
                     /gene="tsaE"
                     /locus_tag="IVW65_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7900493.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7630..8610)
                     /gene="pta"
                     /locus_tag="IVW65_001615"
     CDS             complement(7630..8610)
                     /gene="pta"
                     /locus_tag="IVW65_001615"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7900494.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8763..9443)
                     /locus_tag="IVW65_001616"
     CDS             complement(8763..9443)
                     /locus_tag="IVW65_001616"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7900495.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9569..10438
                     /locus_tag="IVW65_001617"
     CDS             9569..10438
                     /locus_tag="IVW65_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7900496.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10464..10979
                     /locus_tag="IVW65_001618"
     CDS             10464..10979
                     /locus_tag="IVW65_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900497.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11026..11589)
                     /locus_tag="IVW65_001619"
     CDS             complement(11026..11589)
                     /locus_tag="IVW65_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7900498.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11644..11823)
                     /locus_tag="IVW65_001620"
     CDS             complement(11644..11823)
                     /locus_tag="IVW65_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900499.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11900..12409)
                     /locus_tag="IVW65_001621"
     CDS             complement(11900..12409)
                     /locus_tag="IVW65_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900500.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12505..12885)
                     /locus_tag="IVW65_001622"
     CDS             complement(12505..12885)
                     /locus_tag="IVW65_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7900501.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13215..13964
                     /locus_tag="IVW65_001623"
     CDS             13215..13964
                     /locus_tag="IVW65_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7900502.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14148..15425)
                     /locus_tag="IVW65_001624"
     CDS             complement(14148..15425)
                     /locus_tag="IVW65_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900503.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15504..16148)
                     /gene="pcp"
                     /locus_tag="IVW65_001625"
     CDS             complement(15504..16148)
                     /gene="pcp"
                     /locus_tag="IVW65_001625"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7900504.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16347..17291)
                     /locus_tag="IVW65_001626"
     CDS             complement(16347..17291)
                     /locus_tag="IVW65_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7900505.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17293..17979)
                     /locus_tag="IVW65_001627"
     CDS             complement(17293..17979)
                     /locus_tag="IVW65_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7900506.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         5290 a         3698 c         3130 g         6031 t
ORIGIN      
        1 ctttgaaaaa aacataaatc agctaataaa tatgtgagac aaagctgcca tccataaata
       61 ctagctgatt ttttcagtta aatgtctatt tttttctctg atgatggtat ttccaaaata
      121 ttcccagtca tctggagtcg gtggattttt ccaaattatc tgttctttat taaatttttc
      181 aagtaaacaa tcagataaat aaatattcgt acgagaagtg attcgactct caatcacaat
      241 attgtaattt ttaaatcctt tcacttgaga aataatgtaa tcggtataag cagatccttg
      301 cgaaaaatca atacagacat gaatcggtgc ttcaaaatat gataccggac aaataccgac
      361 caataaaaag agataatcat aaaataagcg aatcatgaaa cgttcatctt ttcccaagaa
      421 atagcgttta ttctcttgta tgaccttttg gattgctgta ctgaatattg gatacgtctc
      481 gaagaaaaac tgtagctgct ttttagacgt aaagctagtc atctcaaagt cgaatatcca
      541 attttttcga ttgacatgca tcatacccat acgaagttct tttcgtatat atagtctttc
      601 ttcttcagag atttcaatcg aaaatggtaa ttgatcaatc aattccactg acattttttg
      661 actaatcatg tggatatcat gaaaatgagc ttccgcaagt ccaaatgcgc catctaattc
      721 aaattcttct tccgtgatca aaaacaacat gatataagaa atctcatcaa tgatctgctc
      781 tttagggata tgaagaaaag aaatatgtga taaatccttg atgatcttca ttccttctga
      841 gctcttaaag tattcatctg aaaagaaacg ttttccgatt ggttgattcc cataggccct
      901 caacaaacta atagtggtta gcaaattgaa tttgattcgt tttgttgcag acagttttaa
      961 attgaacaga tattcaaaat attgcacgat actctttgct tcattcatga cgctttctct
     1021 aaatggcata gaaaaaccat taaagacccc gaaatatacc ccaaataata cgttccgtat
     1081 gtttatttct tccccaacta atatgttttt ctttaacttt atttgatgct gttcaagaaa
     1141 agagactaat tctcttcttt ttacataggc tcttgatatg cttaaaaaat gattctctgc
     1201 aaatttttcg atcgttactt ttgaatccac gatctccgtt aacagtcgag caatttttga
     1261 tcgttgaaag tagattcttc ttaaattttt gatatccgtc aaattcagac gttcgacatg
     1321 gatctctgct tcttctgaaa tttggatctc tggctttgtt ttgaattcag taaattcttt
     1381 atttaattct atgatataat ttcttgtttt aaatttagac aaacctaatg tttcatataa
     1441 cttaggcagc actacataat tctttttctc aaattctaaa tatctagcta gcagcagttt
     1501 ttctctatct tcctcttcta taaggtaatc caacttcata tcctacacct caaaaaatgc
     1561 ctcataccaa ttacttgata tgcagatgaa agttcatctt ttcacctata ctgattttat
     1621 gatggattat gcttgttttt tcatcagtta ttctcatttt ttatcttttt tttgagaata
     1681 ttgcaaaaaa tggaaaaatt tatgaggaaa tattctatat tttttgtttt attgtgttaa
     1741 aatgtacaag atttgcgcca tttgcgtaaa caaccacaaa aaagagcagg acggccaccg
     1801 tcctgctctt ttacttattt taagttccat tacttattaa ttggctaaac ttctgcttgt
     1861 ttctgatatt gcttgttttt ctccaatgat ccgcacttct gtttctaaag acacatcaaa
     1921 tttttctttg attacttctt ggatgtgcgc aatcaattca atataatctg tcgctgtcgc
     1981 atgatcgata ttgacgatga atccagcatg tttttcagag acttgtgcac cgccccattt
     2041 caggccttgc aatcctgctt gttggatcag ctgacctgta tagtgtccta ctgggcgctt
     2101 aaagacactt ccgcatgaag ggtattctaa tggctgtttt gattgacgca attcggtcaa
     2161 ctcatccatt cttgctttga ttgcttcata atcccctgtt tgcaaactga atcgagcttc
     2221 caatacgata gcacgtaatt cttggacttt actgtgacga taagaaaata ccatttcgtc
     2281 ttttgtcaaa gttttcaatg ttccatcttc aagcaaaaga tccacttcag caaaaacatc
     2341 tttgatctct ccgccatacg cacctgcatt catataaacc gctccaccga cacttcccgg
     2401 gatgccgcaa gcaaattcaa atccagtcaa tgattctgcc aaagctgcat aagtcgtatc
     2461 aatcagtgag gcacctgctt ccgcaatgac catcgttcct ttgacatgaa tctgtttcat
     2521 ttcagtcagc atgatgacta caccgcgaat cccgccatct tgaacgatca gattgcttgc
     2581 atttcctaat accatccaag gtatatcatg gattcggcaa taatcgatga tttgctttac
     2641 ttcttcttgt tttttcggga atgcaaggac atcagctggt ccacctgttt tcgtaaacgt
     2701 atagttcatc aatggttcat caaacaatat agtaatcgtg gctaagcttt caactatttc
     2761 ttctttattc actgaggatt ttcctttctt ttctcaaacg tcctcattat tctatcacgc
     2821 ttaatacaat cttgccagtc ttgttcaaag ttgatgacgg tagtgatctt taagcacttc
     2881 ctcacataat tcatgacttc ttacaacttc tttttgttta agcaattctg tttccgattt
     2941 acctaaacgg atctgagaaa agaaggcctc taccattggc tcaaacccgc gtttgactag
     3001 cgtgtttgtc cagtcgccaa attctttcac tttttttcca tcagcatctt gaatgacaag
     3061 ctggggcaga tcgtttactt cataagtacc atttttactt gttacttgat atttttctat
     3121 attagcgcca cttgctaaat ccatcgttaa aatagccgtt tgattttgtg tttctaattg
     3181 taaaatggca tattccaaaa attcctttgt ttctcttatc tggtattttg ttctcaaaat
     3241 gggttcatct aataaataca cagctgtatc aataagatgt aaaaacaaat cataaaggac
     3301 gaactctgtc gtttcttttg ccgctattcg atttttttgc aggtggatca accgcttatc
     3361 tgggatacgt ttcaattctt ctaccatagg agcaaagcgc cggttgaaac cgatcatcaa
     3421 aattatttgg ttcttatcag ctaattcttg caattcacga acttcattta gatggacact
     3481 cagtggtttg tcaatataaa catggatacc attttctaga cattttttgg ctaattcata
     3541 atgtactttt gttgctgcgt ggatcataca tgcttcgatt ttttcttcta aaagctcatc
     3601 gatcgtttca tatagatgtt ggaacccgta ctctttttga attctttctt ttgtttcttt
     3661 atttcttgtt gcaaaataaa aatcaagtgt tccttgtttt ttcgcataag ttggtaaata
     3721 tgctttttct gcgatattcc ctacaccgat tacaccaatc ttcatctact ttcctcctgg
     3781 atcatttagt tactttcatc ttacaccatt ttttactaaa atgaggtaca atagtatcgc
     3841 aaaggtaagg aaattaattc gaaggagtgt gcttttattg aaatttatct cttggaacgt
     3901 aaacgggctt cgtgccatcg taaataaaaa ttttttagaa gtttttcatg agttagacgc
     3961 cgattttttc tgtctgcagg aaacgaaact ccaagcagga caaattgatt tagatcttcc
     4021 aggttattat caatattgga attacgcgga acgaaaaggc tattccggca cagcgatttt
     4081 tgctaaaaaa ccagcattga atgccactta cggaatgggt atcgatatcc atgatacaga
     4141 aggccgtctg attactttgg aatattccga tttctttctt gtaacctgtt acacgccaaa
     4201 ttctcaaagc gaattaaaac gtttagatta ccgattagaa tgggaagaag ccttttataa
     4261 ttatttggaa aatctaaaaa aacaaaaacc agtcatcgtg tgtggggatc taaatgtcgc
     4321 tcatcaaaag attgatttga aaaactggaa aacgaatcaa aaaaatgctg gattcactcc
     4381 agaagaaaga gcggctcttt ctcgactgtt ggataacggg tttatcgata cattccgtta
     4441 tttttatcca acccaggaag gtgtctactc ttggtggaac taccgattca attcaaggaa
     4501 aaacaatgct ggttggcgga tcgattactt tttgacgagc aaagatttag agccaggact
     4561 agctgatgca aagatccata cggacattat gggcagtgat cattgtccag tagaattaga
     4621 tttgaaataa agaggttgtg aaaaagtcgt ttagtttcga gtattaagaa aaattttgtg
     4681 aaaatggctt tccacatttt gcataaattt ggcataatac cgagaaactg gcttttgaac
     4741 actgtttatc gagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
     4801 aaaaatagtt cctcatattt ttcgtttggt tgggcttatt accgcagata caagcttttg
     4861 aacaccgttt attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca
     4921 aacaaaaata gttcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt
     4981 ttgaacaccg tttattaaga ggttgtgaaa aagctgcact gcatcaagta ataagaacag
     5041 ccaaacaaaa atagttcctc atatttttcg tttggttgga cttattaccg cagatgcaag
     5101 cttttgaaca ccgtttattg agaagttgtg aaatcagcgt cctgacctga gtattagaga
     5161 aacaaatggc aaaatagttc ctcatatttt gctatttttt gagctaatac cgagggtcag
     5221 ctgattgaac accgtttagt ggagaagttg tgaaaaagtc gcctactttc gagtcgtaag
     5281 aaattttttt gaaaatggtt cctcacaact ttgcataaat ttgacttaat accgagaagt
     5341 catgatcccc atctaactcc aaatcacaag gaatgagttc aaaaactagc ttttctcaac
     5401 aaatattaca taaaaaattc tatgtttttt gttaaattta tttgcttttt tcaaaagtat
     5461 gttacagtaa tttcccaagg aggttttaca gaaatgaatt ttgttgccat ggattttgaa
     5521 actgccaatc atcagcctta tagtgcttgt tctcttgcgc tagtcatggt aaaaaatagc
     5581 cagatcgttg atgaattcta tacattgata cagcctgaga ctcctttttt ctggcgaaat
     5641 gtccaaattc acggaatcca tcaagaagac gtacgcaatg cgccgaaatt cccagaagtc
     5701 tggcaaacta tccaaccata ttttcaaaaa aatcgtttag tcgttgcaca taatgctgct
     5761 tttgatacaa aggtcttagc gggatgctta gattattatc atcttgaaca gcctaattat
     5821 ctttctttat gtacagtgaa aagtagccgt cgcctgtttc ctgaatttcc aaatcatcga
     5881 ttgaacactg tgtgcgaaaa tttgaacatc caattagatc atcaccatga tgcattggaa
     5941 gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa aaaaattcgg gacagatcca
     6001 ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg aaacacacaa agaggtcgtg
     6061 agcctgccgt ttcactccga gtcacaagga gcaatttcaa aaaatagctt ctcatgttta
     6121 ttaaatattg tgactggtga ccgaggagct ggcaggagaa caccgtttat tggagaggtt
     6181 gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
     6241 ttttcatttg gttggactta ttaccgcaga tgcaagcttt tggacaccgt ttaccaagag
     6301 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggcaaaa tagttcctca
     6361 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttattcaaa
     6421 aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc tgccttctca
     6481 aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat aactcatttt
     6541 atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga ccgcttcgat
     6601 ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt gaacgtcaag
     6661 ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt ctagcataag
     6721 agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt ctcccacgtg
     6781 agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg tcccttcata
     6841 gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact cttcttctaa
     6901 ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg tctccttatt
     6961 aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt cccgcaagat
     7021 gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc tgtttgaagg
     7081 tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa tctgtatcac
     7141 ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat agatttcccc
     7201 attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa tctgctccgc
     7261 tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga ctatattctc
     7321 taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct tgggcaattc
     7381 ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga ttgtcacctg
     7441 gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat cctgatagtt
     7501 cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg cgtaatgaag
     7561 tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg gctagttcgc
     7621 gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt tatatacatc
     7681 ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt gtagaattgg
     7741 tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat ttcctgactg
     7801 caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg gcgctttcag
     7861 ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga tcatgtattg
     7921 tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt ctggcgattt
     7981 cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat cgaacaattc
     8041 tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg gattcacgtt
     8101 gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca tgatcatcgc
     8161 tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg ggcgaacagt
     8221 gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca tatacgtaag
     8281 catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt tggatgcctt
     8341 tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt aattatcagg
     8401 gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat ttcttatttc
     8461 ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc gggcagctgc
     8521 gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac ggcgaacaat
     8581 tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt ttatatgtat
     8641 tatcatacac tattccagaa aaaaagaaca gtttatttta tctgtatcag tcaaaaatgc
     8701 tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa agcgctgttt
     8761 tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc aaggagcgca
     8821 tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga taacggactg
     8881 ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat tttttcttgt
     8941 gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa tttttcaatg
     9001 atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg cccttcccga
     9061 acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac tagatttcca
     9121 tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt tcttaacgat
     9181 ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg attttctcca
     9241 tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt caattccaat
     9301 gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc tttttttaaa
     9361 aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac ttcttgccaa
     9421 ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata actcacattt
     9481 tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt aaactaggaa
     9541 caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag atatggacgg
     9601 cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga tacgttttgc
     9661 aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag aagctcgcgc
     9721 agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg cacaggtatt
     9781 tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag caatctctgt
     9841 tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg atggtcttta
     9901 ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta cccatttacc
     9961 tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt tattgcacat
    10021 cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag ttttaaaaat
    10081 tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc agatttctct
    10141 tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga accataaaaa
    10201 cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt ctttagaaga
    10261 agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag caggagtcag
    10321 tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata caacggacac
    10381 aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag acttgtagtt
    10441 ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag cagcttggac
    10501 gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg atggttggtc
    10561 gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat ctcgttgctc
    10621 acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa gagttcgaaa
    10681 aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat attcgtagac
    10741 ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat catttcaatc
    10801 agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat gtattggatg
    10861 tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta gattactggc
    10921 tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac ttaagttaaa
    10981 aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga tattcttttg
    11041 catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga agtcatggcg
    11101 ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga tgacggagcg
    11161 attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga tgccttgtcc
    11221 tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa acgtttcttt
    11281 atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag gtaccagtgg
    11341 ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg caactttacc
    11401 gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat cagtgattgc
    11461 ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg gcagttccat
    11521 atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt ttctcgcaaa
    11581 acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga aatcgcaaaa
    11641 tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag taacgattct
    11701 tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat tatgttcgac
    11761 tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt tcattttttc
    11821 catgttatct caacttcctt tcatctttac tctcttagct tactcttata ttgtaaaaaa
    11881 gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt tctccatcta
    11941 ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac tctttcactt
    12001 ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt tgtttgaact
    12061 cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca tttcgtacgg
    12121 ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa aactggtatt
    12181 catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga atagcagata
    12241 tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat gagatccgat
    12301 attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc gtcataccgg
    12361 taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt cttcattctc
    12421 ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga atgaaacgaa
    12481 aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc cgtctggatc
    12541 aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt ctgattcgat
    12601 ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga aagcaagatg
    12661 acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa ttccaaaaat
    12721 ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt ctttgcggta
    12781 attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg atttctgata
    12841 atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag cactcctatt
    12901 cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg tcagattgaa
    12961 aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa agtaggtcct
    13021 ccaagacact cttcctgttc ccaatataat aaatgaaagt aagaaattat attttcaaga
    13081 aaacaaacag aaaattatca taacacaaat tatttataat tgaaagacta gttttttttc
    13141 aagaaaagca tacaatagat tgtgttagta aatacagaac acactacatt tagtgtctta
    13201 aggagtcctc atacatgaag tcatattttc agtttttagc tcatcagcta aaaggctggc
    13261 cgcaacaaaa ttattatctt ttctttttca gtcttggctg ccaggtcatg actcttgtca
    13321 atagtcccat cacttggctg tccgttatta catttatcgg taccacgctt ggtgtattgt
    13381 gtattttatc catcaatgcg gcaaaatcag tcaacggtat tttaggtatt ttgtctgcga
    13441 tttgttttat tatcgttggg ttttcagcaa agaactattt aagtatcggt gaacagattg
    13501 cttatatgat cacattggat atccccgtat tgttaagtaa agaatggaat cataatatgg
    13561 cttcaaaaat ccgaaaattc actggaaaaa gctggtttat cgcaatcatc agtactttcc
    13621 ttgtctatct tgtttcagga ttcctgattg gtcgttatac agatgatcct cggccgtggg
    13681 tagatgccat tgctttttct attagcttga ctgctggtat catcagtttc ttgcgttaca
    13741 acaaccaata ttactggtgg ttagcttcag ggcttgctca aatggtcctt tggtatattt
    13801 catttacgca tggatctgca agtttggcga tgttcgtgaa cagctctgtt tacttgctga
    13861 atgatgtcct tgcatttacc gtttcacctt ggtataacca aaaagaacgt gaacgtatgc
    13921 aaaaaatgga agaagaatac tacaacgcac aacaacacgc ataaaaaaac tgagcgtggg
    13981 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaatcagaa
    14041 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
    14101 tcttcttatt tcttggagtt aaacgcttct gtcccagcct ctatttttta tagtaattcc
    14161 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
    14221 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
    14281 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
    14341 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
    14401 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
    14461 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
    14521 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
    14581 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
    14641 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
    14701 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
    14761 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    14821 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    14881 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    14941 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    15001 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    15061 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    15121 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    15181 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    15241 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    15301 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    15361 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    15421 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    15481 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    15541 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    15601 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    15661 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    15721 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    15781 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    15841 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    15901 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    15961 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    16021 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    16081 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    16141 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    16201 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    16261 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    16321 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    16381 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    16441 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    16501 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    16561 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    16621 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    16681 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    16741 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    16801 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    16861 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    16921 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    16981 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    17041 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    17101 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    17161 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    17221 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    17281 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    17341 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    17401 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    17461 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    17521 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    17581 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    17641 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    17701 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    17761 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    17821 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    17881 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    17941 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    18001 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    18061 agacactatt tttaaatatt tttcgttatt tttttatcac aaaaattatc ttacttattg
    18121 taaggtaaag ctttttttaa tttattgcc
//
LOCUS       DACCWK010000050        17763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCWK010000050 DACCWK010000000
VERSION     DACCWK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.050"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(68..841)
                     /locus_tag="IVW65_001628"
     CDS             complement(68..841)
                     /locus_tag="IVW65_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900507.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            1108..2808
                     /locus_tag="IVW65_001629"
     CDS             1108..2808
                     /locus_tag="IVW65_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7900508.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2778..2996
                     /locus_tag="IVW65_001630"
     CDS             2778..2996
                     /locus_tag="IVW65_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900509.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            3016..3906
                     /locus_tag="IVW65_001631"
     CDS             3016..3906
                     /locus_tag="IVW65_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7900510.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3924..4208
                     /locus_tag="IVW65_001632"
     CDS             3924..4208
                     /locus_tag="IVW65_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900511.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4695..5309
                     /gene="gmk"
                     /locus_tag="IVW65_001633"
     CDS             4695..5309
                     /gene="gmk"
                     /locus_tag="IVW65_001633"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7900512.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            5314..5619
                     /locus_tag="IVW65_001634"
     CDS             5314..5619
                     /locus_tag="IVW65_001634"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7900513.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5872..8280
                     /gene="priA"
                     /locus_tag="IVW65_001635"
     CDS             5872..8280
                     /gene="priA"
                     /locus_tag="IVW65_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7900514.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            8295..8786
                     /gene="def"
                     /locus_tag="IVW65_001636"
     CDS             8295..8786
                     /gene="def"
                     /locus_tag="IVW65_001636"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7900515.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8779..9717
                     /locus_tag="IVW65_001637"
     CDS             8779..9717
                     /locus_tag="IVW65_001637"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7900516.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9717..11075
                     /gene="rsmB"
                     /locus_tag="IVW65_001638"
     CDS             9717..11075
                     /gene="rsmB"
                     /locus_tag="IVW65_001638"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7900517.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            11088..11828
                     /locus_tag="IVW65_001639"
     CDS             11088..11828
                     /locus_tag="IVW65_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7900518.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11825..13894
                     /gene="pknB"
                     /locus_tag="IVW65_001640"
     CDS             11825..13894
                     /gene="pknB"
                     /locus_tag="IVW65_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7900519.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            14059..14958
                     /gene="rsgA"
                     /locus_tag="IVW65_001641"
     CDS             14059..14958
                     /gene="rsgA"
                     /locus_tag="IVW65_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7900520.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14971..15621
                     /locus_tag="IVW65_001642"
     CDS             14971..15621
                     /locus_tag="IVW65_001642"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7900521.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15622..16260
                     /locus_tag="IVW65_001643"
     CDS             15622..16260
                     /locus_tag="IVW65_001643"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7900522.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            16415..17269
                     /gene="mreC"
                     /locus_tag="IVW65_001644"
     CDS             16415..17269
                     /gene="mreC"
                     /locus_tag="IVW65_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7900523.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            17273..>17763
                     /gene="mreD"
                     /locus_tag="IVW65_001645"
     CDS             17273..>17763
                     /gene="mreD"
                     /locus_tag="IVW65_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7900524.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFK"
BASE COUNT         5950 a         3205 c         3822 g         4786 t
ORIGIN      
        1 aagaaacttg agacagaatc atgtcctagt ttcttgtttc ctttttattt tttgataaga
       61 aatagatcta cttcaatggc agataagaaa gatcggcatc ttctaccaat tgccagtgtc
      121 gatcggtgac agagtagatt aaagtagcga tgatttcctc ttctcgatac cgggtgcctt
      181 ttaatttcca aatataatct tcaatcacat agattgggat acgatattta ccatcgatca
      241 aaccaacttg atcaaaagaa aaaatgatcc cttcctgcat caaattacgc atttctttca
      301 ataattccac actgattttt tcaacaggtg tttctttttt gaaaaaagat ttttttacat
      361 tgatcttatc tgacaagatc gaacttaatt tactagcaaa aatcctataa aattcatcta
      421 aatagcgata ataaatccga ttcagctgat cattaagatc gaagtataca aaattattct
      481 gtagtttata aaaaaacggc gaatgaagat gtgttttcat atgaccaaaa tataataatt
      541 ctgaaatttc taaaggtgtc aattctttca gcatcgttaa atcagtaaag tcgatccact
      601 tcaattcatc gaatccacgc ctgttacgag aagaggaaaa gaaacgctga atattttctg
      661 taccacggat tactttcagc cctgtatggg attcatattc tccagcagaa gcagatggat
      721 caagcaagag caagttttga ggaaaatgca cgatactgtt tgcaaagtca gtagcagtca
      781 gcccttttgt taaaacagca ttacttgtgg tatcaatatg tacataaact gactcagtca
      841 tcatgttcct cctcttattt cctcttaatc tcttttattt tacaacattt gcattaaaaa
      901 agcttgtttc ttttatttta aaattccatt acaaaatcac gttttcattg aaaacactct
      961 cattttcaat taatttttgt gatttttgta tttttttatt taaaggaggt tttatgaatt
     1021 gttttcttat ggaatctaca aagtcgaata cgagactcat gaccttttta tgacgtactg
     1081 gtatacttga agaaaaagga gagcgcgatg gagataaaaa aagtttactt ttcacagaaa
     1141 atgtcgctgt attcgcctat ccagcaagca aaacaacccc ttccattttt tcaaagtcat
     1201 caagaaagta gagacgcttt ttggcgttct gcaaaaaagc aatgtgcttt taaagcagta
     1261 tcgaaaaaat ataccgtcta ttttactatt gctaaaagta gaggagatgt tatttttaat
     1321 aatctgttga ttgaaggtga aacttttgaa tggaaaaaat tttttcggta tatggaagct
     1381 tgcgctcgtt tttttattaa agaaaactgt tgttttttat ttcaatctcc tctatcagaa
     1441 gaatggttgc gtatttttca taaaaacggc tatcaaggaa ccacacaatt aaataaaaag
     1501 ctggtctatc atacagcact tgttttaggt ggaggaggag cgcacggtgc ttatcagatt
     1561 ggtgtgtggc aggcgctgaa ggaacatgat atcaactttg agattatcac aggtacctcg
     1621 gtaggtgcgt tgaacggcgc attgatttta caaggagata tgaaaaaagc tgtgaattta
     1681 tggaaaaagc tgtcgacaag acaagtgctt gcgttaccag aaatggctgc agttgaagac
     1741 cttcgtgaac gcttctatcg agaaagacgc caaatgacga aaacagcact aattgaaggc
     1801 ggggtttctt ctgctccatt ggaaaagatg gtcaaagaca atttggattt gtccttactc
     1861 aaacataatc ccaaaatacg tttatatacg gtgtctacta aactgccaga actagcggaa
     1921 gtagtgactg atatccaaca gacaaaaaca gaagaaatac cagattggat actcgcctct
     1981 gcttcatttt atcccgctat ggcttatcga aagatcggaa aaaataaata tgcagacggt
     2041 gggtatcgca acaaaatacc tattgatatt gcgattaaca aaggagcaac agaggcattt
     2101 gtcgtagatg tgcaagggcc aggcccagcg aaaaaaattc gtatgccaga catttttatc
     2161 cattggaaat gtcaaacatt gtggacacta ggcagctttc tgctttttga tagccaaagg
     2221 aaccaattaa atttacaatt aggctatctg gaaatgaaaa aacgattagg ctattactat
     2281 gggaactggt atacatttca ttctgttaaa cctgcaagaa catactggcg aggatttttg
     2341 tcttacttaa caaaagaaat tcagttagaa ttatcttttt tcaaaagcat aaaattttgg
     2401 caaaaactgc gtaatttata taagaacagg gtcgtaccag agacatgcgg attggcgatg
     2461 ctagaacttt tagcaaaaaa acatttttta ctgcccaatg aaatctatca agtagacatg
     2521 atgattcaaa tgatctgcca acaagatata aaaagtatgc cggatgattt attggcacaa
     2581 attggtcaat taagtactga agaatggcga aggtatcgaa aataccagca aaaaatacaa
     2641 aatgagcgca cgaaaactgt ttattttgat tatctgctac aaggaaaacg caaggatcgt
     2701 ttgcagcatg aattgcttaa gcaaccagtt gacacattac tgatcctata tttatattat
     2761 ctcaaggagg aacaaccatg gcacaagaat tttcatatga aatcgtagaa gaaatcgcta
     2821 tattgtcaga aaacaacaag gggtggcgta aagaactgaa cctagtcagc tggaatggtc
     2881 gcccgccaaa attcgatctt cgagattggg cgccagacca tgaaaaaatg gggaaaggcc
     2941 tgacattgac gaacgaagag tttgaacaac tgcaaaaagc aatagaaaat atgtaatgag
     3001 gaaggaccct gtcgaatgaa aagtatgact ggttttggca aagccatccg cgaaacaaaa
     3061 gcgtatcagt tagatattga aataaaaagt gtcaaccagc gttttctaga tgttcaaata
     3121 cgaagtccta aaatcctgaa ctttgttgaa aacgattttc gtcagcttat taaacagcag
     3181 ttaagccgcg gtcgaatcga agtatttgtg aatctagctt atattggcaa aaacgaaaaa
     3241 cagatcgtca tcaactggga attgattgat tcacttatca ctcaattgac agacggaatt
     3301 caacaacgat acggtagtca aaatcaagtg tcaattagtc ggatggcgga acgcttcgtg
     3361 ctggacgaaa acttcgtaac ggtagaagaa aaaccggaag agagcatgga agaattggca
     3421 cagttagctc tagatacatt gaaaagcgca cttgattcta ttgaggcgag tcgagaaaaa
     3481 gaaggtcaag ctttagcttt agtgttgaaa aagaatactg aagaattcaa agaagtacta
     3541 aaaaaactcg atagttttgt agaaatctat gaaaaggaat ttcaaataag gtatcaaaaa
     3601 aagctggagg aatttcttgg agcgaaagtc gatgagcaac ggttgttgac ggagctagcc
     3661 atcttgttgg aacgcggaga tattcacgag gaactggatc gtctggcgat ccatgtacaa
     3721 aaactagatg aacttatcga agcacaatgt cctgtaggtc gggaattaga tttcctgatc
     3781 caagaaatga atcgggaaat caacacgatc ggttccaaat caaatgcaat cgaaattaaa
     3841 aatcaagtcg tccagtctaa gacgatctta gaaaaaatca gagaacaagt acaaaatatt
     3901 gaatgacgaa agaggtagag ataatgaaat attatgacgt aacgttccac gaattaagtg
     3961 gaaaaactgt ggttaaacgg gatatccctt ctgaaaaaaa cggctttgac gtatggaaag
     4021 atgcctgtgc agactataat gagaacgaac tgtttatttt gatcaacgac ggtgcttacg
     4081 tgacgatgaa tcggaaattt attgttcgaa ttgacacaga agaggtagaa gatccgacag
     4141 aaaaagcacg ttctcgaaaa gatgagatca tgggtgtagt gaatactttg tccaatatgg
     4201 gcttttagca aggatgctga acaaagaaat acaataaata aacagaattg gatttcttcg
     4261 cttagcttct ttgctctagg aatacattta aaggttgtga caaaattcgt gtcacaatct
     4321 ttttttattt tgaataagtg atgttcaaaa accattttct cggtattaag ccaaatttat
     4381 gcaaaaatgt gagaaactat tttcacaaaa tttttcttca tactcgaaac taatcggctt
     4441 cttcacgacc tcttatacac ggcgttcaaa agcttgcatc tgcgataata accccaacca
     4501 aacgaaaaac atgagaagct gtttatgttt ggctgttctt attacttgat gcaggacagc
     4561 ttttttacga cctcttggta cagggtagct tttttaccaa tttctagcta taattaaaga
     4621 taaccttgaa agttgcagaa aaaaagtgca aaataaacga taagtacaaa aagactagtt
     4681 gaaaggacgt cgtcatgtca gagcgtggat tattaatcgt gttgtctggt ccatccggtg
     4741 taggaaaagg cacagtacga aaagcaattt ttgagagtga ggataatgat tttcagtatt
     4801 cgatctcaat gactacccgt aaaatgcgtg aaggggaggt cgaaggcgtt gattatttct
     4861 tccgaagcaa agaagagttt gaatcaatga tcgaagctgg agagatgctg gaatacgcag
     4921 aatatgtcgg gaattattat ggaactcctt tgtcatatgt caacaagaca ttggatgaag
     4981 gaaaagatgt tttcttggag attgaagtcc aaggagcaaa acaagtcaaa gaaaaagtgc
     5041 ccgatggtgt attcatcttt ttgactccgc cagatttagc ggaattaaaa tcacggatcg
     5101 taggccgagg aacagatgca gatgaagtaa tcgaaaaacg aatgaaagtt gcaaaagaag
     5161 aaatcgaaat gatggctctt tacgactatg ctgttgtaaa tgatgaagtg ccgctagcag
     5221 ttgaacgaat caaaaacatt attgccagtg aacattttcg agtagatcgc gtaatcggta
     5281 aatatattaa aatgttaaag gagatgtagt cttatgatgt taaaaccttc aattgactca
     5341 ctattagatc gtgtaaattc aaaatattct ctagtgattt tagcaagtaa acgcgcgcat
     5401 gaattggatg caagtgcaca gcctactcta gattcttttg attcagtaaa aagcgtcggt
     5461 caagctttag aagaaatcga tgcaggaaat gtagtcaatg atccacatcc tgaattaaaa
     5521 cgtgaacgtt tgaagatgga agaagaggaa cgtcaagcac aaaaagagcg tgaacaacac
     5581 gaattggaat cacgtatccg tgaagagcaa aaaatgtaat aaagaaaaat agagcgaaca
     5641 tctaaaaaga aaagcaaacc cagctgtttc cttttcaaac attcgccact tattgtctgg
     5701 tagaaaaggc tgtgtctgaa ctttttgaca cagttctttt ttttcttatc tgctgttaat
     5761 tttttgttag gatttggcag aagataagac aaatccatcg atctctagta aaataggcaa
     5821 ggaacttttt gtcgcttgat aagaaaagac aaaaaaagga ggaaacgaac catgacatta
     5881 acagctgaag tcattgtgga cgtacctact atgcagaccg atcagccatt cacttatttg
     5941 gttccctctg aaatggaaac agctttgcaa gtcgggatgc gagtagaagt cccttttggt
     6001 aatggcaatc gtcatgtcca aggatttgtt atggctatcc aaaaatcaga agaatcagct
     6061 aatccatctt tgaaggcagt tattcgtttg ctagaccttg ctccggtggt caatgaagaa
     6121 ttactttcat tagctgatta tatgaaaaaa attacttacg cattcaaaat cacttgtcta
     6181 caaacgatgc ttccgagtgt gatgaaagca gaatatgata aattgatcta tcctttggca
     6241 gacacaccag aagttcaggt attgtttgat cagcgagaag tgatttcctg gaaggaagca
     6301 gaagaagaag gcagcctttc tcaattgatt cgctggcgcc aagagcaatt agtagatatc
     6361 aaatatgaag tccatacacg aaataaagta aaaacgattc gtttggtccg ttctttgtta
     6421 actgaaaagc aaatcgaaga agaatgggca aaattacggc aaaatgccaa gaaacaaaaa
     6481 gaacttcttt tgtgtttatc tgaactgagc caagaagaac caattgctta tttcaaaaat
     6541 aaaggaatca gtactgctgt cttgaatcaa ggaaaagaaa agggctggct ggagtttata
     6601 gaatcagaac gttatcgcga cccttataaa gaccgagtat ttgaccaaac gacggcattg
     6661 gaactgaatg cagaacaaaa aaatgctgtc gaacaaatca tcacagcagg tcagcagcaa
     6721 aaagatcagg tctttttgct ggagggaatc actggcagcg ggaaaacaga agtatatctt
     6781 caggcaatcg ctgatgtttt gtcagaaaat aaaacggcta ttatgttagt gcctgaaatc
     6841 gcattgactc cgcaaatggt cgagcgattc aaaggacgct tcggagaatc agttgcggtc
     6901 ttacatagcg gactttccca aggtgaaaaa tatgatgaat ggcgcaagat tgagcgggaa
     6961 gaagcgcaag tagtagtagg cgcacgatct gctatttttg ccccgctgaa aaatatcggg
     7021 ctgatcatta tcgatgaaga acatgaatca agttacaaac aagatgagac accgcgttat
     7081 catgcgagag atctagcaat ttggcgcagt aaatatcatc attgcccaat agttctagga
     7141 agcgcgacgc cctctttaga atcaagagca cgagctcaaa aaggcgttta tcagttattg
     7201 cagttaaacc accgagccaa agcagctgca cagcttccag ctattgagat cgtagatatg
     7261 cgtgaagagt ttcagaatca tcggacctct actttttctg ctaatttgca ggaaaaaata
     7321 caaaatcgat tagacaaaaa agaacaaact gtcctactgc tcaatcgtcg aggatattcg
     7381 tcttttgtga tgtgtaggga ctgtggattt gttcttccat gtcctaattg cgatatctct
     7441 cttacactgc atatggatac acgttcaatg cgctgccatt attgcggaca tgaagaacct
     7501 attcccaatc gctgtcctaa ttgtggcggc aataaaatcc gctactatgg aacaggtact
     7561 caaaaagtag aagaggaact ccgagaactt tatccgcaag ctaggatttt gcgtatggat
     7621 gtagatacca ctcgaagaaa aggggcacac gaacagattt tacaaaaatt cggtgcaaaa
     7681 gaagcagata tattattagg gacacaaatg attgctaaag gtttggactt tccagaagtg
     7741 acattagttg gggtgttgaa tgctgatacc tccctgaatt taccggattt tcgttctagt
     7801 gagcatacat tccaattgct tacccaagtt tcaggacgag cagggcgagc ggaaaaagca
     7861 ggcgaagtcg tgatccaaac attcaatcct caacattacg ccatagaatt ggctaaaaaa
     7921 caaaattatg agcagtttta tcagcaggaa atgcatgtac ggcatcgtgg aggctatcct
     7981 ccttattact ttactgtgaa aataaccgcc agccatcctg aagaacaagt agcggcaaag
     8041 aaaatctttc agattgccaa tcagctaaaa gaagtgttaa gccctcaaag cttgctatta
     8101 gggccaacgc ccagcatgat tttgagagtg aaaaaccgat attattatca actaatcatc
     8161 aaatataaac atgaaccgaa tcttcctcat gttttggatg aaattttgaa tggaagccag
     8221 aaagaacagc gacaaggtct ttttgtggca attgacaacg aaccattaaa ctttatttaa
     8281 gaaagggcca aattatgcgc tatcctatat tgatccaccc taatgataaa ttaaaaagaa
     8341 ctgctcagcc aatcgatgta atcacggacg agacaatcgc tttattagac aatttatatg
     8401 agacaatgat tgcgaatgac ggaataggga tcgctgctcc tcaagtagga caaaacaaga
     8461 gaattgctgt gatcgaagta gatgaaggcg agaaatttga actgattaac ccagaaatca
     8521 tcgaagcaaa aggtgaaagc cttgacgtag aaggttgtct cagtattccg catgtctatg
     8581 gaacggtcaa gcgagcagat gaagtgaccg tgcgttatta tgatcgagac ggagaagaga
     8641 tagaagtgac ggcttttggc tatttagccc gtgccttcca gcatgaaatc gatcacttag
     8701 acggtatctt gttcattgag aaaatgatac agcagatccc ggaagaagaa cttgaagaat
     8761 atatggagga acatcttgat gactaagcta gtatttatgg gcacacctgc tttttctgtg
     8821 ccgattcttg aagggctgat agaatcaggt tacgagatcc aggcagtggt aacacagccc
     8881 gaccgtccag ttgggcggaa acgagtaatt acgccgacac cggtaaaaaa agcggctctg
     8941 aagcacggta tccgtgtgct gcagccagag aaaatctccg gttcgccaga aatggaagaa
     9001 atcattgaat tggtgcctga tgtgatcgtc actgcggcat ttggccaatt cttgcctgaa
     9061 aagttattgc aagtaccaaa attaggggca atcaatgtcc atgcttcttt acttcctaaa
     9121 tatcggggag gtgcgcctgt tcattatgcc atcatgaatg gggagaaaga aactggtgta
     9181 acgatcatgg aaatgatcaa aaaaatggat gcaggcggaa tctatgcgca agaaagcata
     9241 ccaatcacta aacaagatga tgtagggacg atgtttgaaa agcttagcct tttaggaaga
     9301 aagctattgc tagaaacatt gcccaatatt ttggatggac aaaaaccagt acctcaagac
     9361 gagtcagaag caactttttc gccaaacatc acacgggaac aagaagcaat cgattggcat
     9421 aaaactgcag aagaaattga caaccaagta cgcggaatgc gtccttggcc aattgcattc
     9481 acgacctatg agggcatacg ctggaaatta ttatcggttg aagtattggc agaaactaca
     9541 gacaaagcac ctggtacgat catccggaaa gacaaaaaaa atctatggat tgcttgcggt
     9601 aaacagaccg tcttagccat caatgaatta cagccagctg gtaaaggaaa acaggccgta
     9661 catgatttcc ttaacggaag cggtcagcaa gttcagattg gtcagcaggt gaaataatga
     9721 aaaaaatccc taaaaaaatg aaaaaatcag tacgatatat ggcattactg acattagagc
     9781 gagtgaacaa gggtggcgcc tactcgaatc ttttgttgaa tgagatgatc aaacaaagcg
     9841 agctaaatca aaaagatgtg ggtttattta cagaattgat ctatggtacg attagccatc
     9901 agcgactatt ggaattttac gttgcaccat tgatcgctaa agcaaaaaaa gtggatgact
     9961 gggtcaagat gcttctttat ctttctgtat accaattaga atttttagat aaagtccctg
    10021 cacatgctat tttaaacgag gcggtcgaaa tcgcaaaagt aaaaggaaat cctgggacag
    10081 ggaaatttgt aaatggtgtc ttgaggaatt atcagcgtca aggagcacca gatctgcaag
    10141 gcattgctga tcctatcgaa agactttctg tcaaaatcag cctgcctcta tggatgacga
    10201 aacgactggt ggatcaaatc ggatacgaag aaactgaaaa gctcggttta tcgctatatc
    10261 agcctagcca tgctagtgcc cgagtagata cacgccgact ttccagagat caggccatca
    10321 ctgttttgca agaagaagag atcacagctg aaaaaagcca agtcagtcca tacggtatag
    10381 tagcgaaaaa agggcattta gcttctagct cattgttcca cgatggcgtc atgacgatcc
    10441 aagatgaaag ttcgatgctt gtagcgccgt ctatgcaaat cgagcctgaa catcatgtct
    10501 tagatgcctg tgcagcaccc ggaggtaaga cgacgcatat tgcttctttt ttagatccgt
    10561 catgcggtgg agaagtggtc gctttagatg tacacgaaca caaagtcaaa ctcattgaag
    10621 ataatgcagc acgtctaggt gtctcttctg ctgtctacgc tcaaaagatg gatgctagag
    10681 aagtcagtaa acagttcgaa gcagagcagt ttgatcggat tttagtagac gcgccttgtt
    10741 caggtcttgg gctgcttcgg cgaaaaccag atattcgtta taaaaaacag ccggatgaat
    10801 tagcgaattt accaaaaatc caattggaaa ttttagaaag ctgcgcacca actttaaagt
    10861 cttccggtat tctgacttat agcacttgta cgatcttaaa agaagaaaac caagacgttg
    10921 tttctgcttt cttggaaaga caccctgatt ttgagaggat tgatgttttg accaatccta
    10981 tgctacaatc atcaatcgaa gagaaaatgt tgactttgta tccgcatcag ttttacacag
    11041 atggattttt catttgttgt ttgcgcaaaa agtaatgagg tgaaaaaatg cagattgaat
    11101 atcaatcaga tgtaggaaga cgacgtaaca caaatcaaga ctatgccagt gtatttacca
    11161 gccgttcagg tctcaagctt gctgtattag cagacgggat gggtggtcat cgtgccggag
    11221 acattgccag tcagatggcg gtaacgaatc taggcgaagc ttgggaaaaa caagaagctg
    11281 ttgacgatga aaaaatcgcc caatggttca ttcaatcgat tcaagaagaa aatatgctga
    11341 tttatcagcg tggacaagaa cagccggaat ataacggtat gggaacaaca atcgtagcgg
    11401 ccgctctgtc agatgactgg ttcacgattg ccaatgttgg ggatagcaga gcttatttga
    11461 ttcgtgaaga taaaatcatg caattgacgg aggatcattc tttagtcaat gaactagtca
    11521 aatcaggaga aatcagcgaa gaaatggcag tgaaccatcc aaggaaaaat attttaacac
    11581 gttctgtcgg catgcctgga acagtcgaag tagatgtttc tactcatctt tggcaactaa
    11641 aagatcgact gctgttatgt tcagatggct tgacgaacat gctgtctgaa gaagaaatag
    11701 gaacgatcat caatcaagaa ggcccattgt ctcataaagt aactgagctg attgatcgtg
    11761 ccaatgaagc aggcggagcg gataatatca ccgttcttct tatcgaatat aaggaggaca
    11821 gtgtatgatc gagttgggca aaaaattaaa tggccgttat caaatcactg gaaatatcgg
    11881 tagcggtggt atggcaaatg tatttttggc gcacgattta atcttggatc gtgatgtagc
    11941 agttaaagtc ttacgatttg actttcaaaa tgaccagact gcgattcgac gatttcagag
    12001 agaagcttta gcagcaacag aactcgttca tccaaacatt gttagtgttt acgatgttgg
    12061 tgaagaagac aacatgcagt atctggtgat ggaatacgtc aaaggaatgg acttgaaacg
    12121 atatatccaa acacattatc ctgttcctta tgaaacagca gtaaatatta tgcagcagat
    12181 cctttctgct atttctttag ctcataacca tcaaatcatc caccgagatt tgaaacccca
    12241 aaacgtactg atcgataatg aaggtgttgt aaaaatcacg gatttcggga ttgctatcgc
    12301 tttatctgaa acatcgatca cgcagacgaa tacgatgcta ggatctgttc attatctgtc
    12361 ccctgagcag gccagaggaa gtatggcaac gaagcagtca gatatttatg ctttagggat
    12421 catcctttat gagatgttga cgggtagcgt accgtttgat ggtgaatctg ctgtaacaat
    12481 tgctttaaaa cattttcaag atgatcttcc atctataaaa gcacttgatc caaatgtgcc
    12541 acaagcactg gaaaacgtga ttttacgggc aacggctaaa gaacctgcag accgttataa
    12601 gtcagccgag gaaatgtctg atgatctttc tacagtatta tcaccagctc gtgcaaacga
    12661 agaaaaatgg cagcctcatg tgatggataa tgaaacaaaa gttatcacac cccttacgga
    12721 agatgcacca atgcctgatt cattcaaatc tatgcctctg ccaaaagaca agccagaaaa
    12781 cgaaacctta ccagaggagg aagaagttct gcctgatcct aaaaagaaag gacagaaaaa
    12841 atggtggctg atcctattag ctattcttgc tgttttagga atcggaacag ctgtattctt
    12901 tgcttctggc gggcgaggag aagttgttgt tcctgatgtt tctgatcttt ctgaagcttc
    12961 tgctagagaa acactcgcaa aagcgaaatt agaagtagca acaaaaacag aagaaattgc
    13021 agatgacaaa atcgaagaag gtaatgtagt gaagacagac ccggcagcgg gaacgacagt
    13081 caagcaaaaa cgcgaagtca ctctgtatat tagtagcggc acgaaaaaaa tcaaattaga
    13141 tgattacagt ggtaaaagtt atgaagatgc cgaagcaagt ttgaaagaat tagggttttc
    13201 tgctgacttg attcaaaaag aagaggaatt cagttcagag gtagaaagcg gaatgatcat
    13261 ttcgcaatct ccatcagaag atacagaagt cgatcctaaa acagacacga ttactttcac
    13321 agtcagcaaa ggaccagaaa cagtgacttt gagtagtcat gtaggagaaa actacgaaac
    13381 tgttcgtaat tggcttgtta cacaaggatt caatatcaat aatattagtg ctacttatga
    13441 ctatagcgac actgtaccat caggttcgat catcagtcag gatccagcct cgggtgaagt
    13501 tgtagcagaa gaaacttaca tcaattttgt cgtaagccaa ggaacagagc ctaaattgaa
    13561 tgatatctcc ggctatacaa aaagcgaagc acaaagttat ttagctagtg tcggtgcaga
    13621 atatatcggg catgagactt ccgaatattc aaacacggtg gataaagata aagtgattcg
    13681 tacagtacca ggggcaggaa cgacaattac caaaggaatg gttgtgaacg taatttactc
    13741 taaaggacca gatccagcgt cttctagttc tgaaaaagac tcttcagaca gttctagcag
    13801 ctcaagtagc tccagtagca caagcaactc ttcaaaagaa gaatcttcca gctccacaag
    13861 ttcatcagct agcacctcag aaagttcaaa ataagtgctg aataaaatat atcagaaggg
    13921 aatgcgacat tgatttgttg cattctcttt tttgtgtttc tatctctttt gatcaatttt
    13981 caagccatat actttgagaa gtttgcatgg aataacacct actttcctct tcgtatggta
    14041 aaataaacaa aaacggaggt ggcacttatc aaaggacaaa tcagaaaagc aatcagcggt
    14101 ttttactatg tatacgcaaa cggagagacc tatcaaacaa gaggaagagg aaatttccgg
    14161 aatcggaaaa tcacgccgct tgtcggagat caggtgatct ttgagagtga aaatcaaaca
    14221 gacggctatc ttctggaaat cttgccgaga aaaaatcaat tagttcgccc accagtcaca
    14281 aatgttgatc ttggaatcat tgtcacaagt atcgtagagc ctgcattttc ttataatcta
    14341 cttgaccgct ttctagttac cttggaatac gagtcgatcg aaccgattat ttttttgaca
    14401 aagaccgact tgtcagacga cccagaaaaa atcgaagaga tcaagcaaac ctatactaaa
    14461 ataggctatc cggtgattgt acccaatcat acgggtgata cgacagaact gatccgttac
    14521 ttcccagaac ggctatcggt ctttatgggt cagtctggag caggaaaatc gacgctactg
    14581 aatcaaatat caccggaact gaatttagca acaggagaga tttctgatgc acttggaaga
    14641 ggacggcata caacacgtca tgtggaactg attcctatct atggcgggtt agtagcagat
    14701 acaccaggat ttagctcgat tgactttttg acaattgaag caggagaact gccgagacaa
    14761 tttcctgaat ttgtagaagc ttctgtccat tgcaaattta gagagtgcat gcatgctaaa
    14821 gaacctggct gcgaagtaaa acatagagta gaaacaggtg gtatcgcaca gacacgttac
    14881 gacaattatt tgcaattctt acaagaaatc gaaaaaagaa aaccaatgta tgcaaaaaaa
    14941 gaaggtaaac gaaaatagga ggaaacaaga atgaagatag caccatccat tttgagtgca
    15001 gattttgcca atttacaaag agacattgag ttagtggaaa aaggaggcgc agattacatc
    15061 cacgtagatg taatggatgg ccgatttgtc ccaaatatca cattagggcc aaatattgtt
    15121 caggcaatcc gtcctgttac taaattaccg ttagacgtcc atctgatgat cgttgatcca
    15181 gaaaactaca ttccagcatt tgcaaaagct ggagcagata tcattactgt ccacgtcgaa
    15241 gcaactcctc atattcatcg ggcacttcaa atgacaaaaa gcttaggagt taaagctgga
    15301 gtagttatca atccaggaac accagtctcc atgatcaaac atgttttgcc tttggctgat
    15361 caagtactag tcatgactgt caatcctggg ttcggcggac aaagctttat tgaagaaaca
    15421 gtagaaaaaa ttacggaact cgctgatcta cgtagagaga acaattggac ttatgcaatc
    15481 gaagtagatg gcggaatcgt tccagaaaca gcaaaaatct gtaaagatgc aggggcggat
    15541 gtcttcgttg caggatcata tatctatgga gccgaaaatc cagtcggaca aatcgaacga
    15601 ttaaaagagg cattagacta aatgaaaatt ttattagctg ctggaggtaa tccgcaaaac
    15661 tggccgcagt ctctgctgtc caaatacgat gcctatgtcg ggatagaccg tggtgcgctg
    15721 tacctgcttc aagcaggcta cccagtagat ctggcagtgg gtgattttga ttcattgtcg
    15781 aacgaagagc agaaggaagt atttgcaaaa gccaaagagt atacccgttc ccctgcagaa
    15841 aaagacgata cagatacaca gttaggtatc ttaaaaacat ttgaacgtta cccgcaagcg
    15901 gaagtcactt tgattggagc tacgggcgga agattggatc atcttttagc caatctctgg
    15961 ttaggtatgg aacctcgctt caaacccttt gcccatcagc ttattatcag agattgtcaa
    16021 aattatttgt cttattattt gcctggagag catatagtaa gacagatacc agatatgaag
    16081 tatttagctt attgctgctt gactcccgtc tcgaatctta cattaaaagg cagcaagtat
    16141 gtattggaaa ataaagaagt agaatatccg acatcttatg caagtaacga atttctttca
    16201 gataccgcag aattttcttt ttcagatggt atgattgcag ttattcaaag caaagattga
    16261 tccataaaca aataaaaaaa tttgtctttc aggctaaaaa tgacagaatc tattatttgc
    16321 taaaaaaaag cttaagagga gcaacttacc ttttgtttca cggcatttag tgataaaatt
    16381 gaacacaaga gtctaattaa ttcgaggtgt ctttgtgaag aagttcaatc ctaataaaaa
    16441 tattattatt accttggtta ttgttatcat cgtggtgacg atactaagtg tgaccgctgc
    16501 gcagcgagcg aatgaaggaa agcccaattt cttccaatcc atcgttaatg acagtgttgc
    16561 aatggttgat aaaacaatct ctgccccagt acggtgggta cgaaatggag tagattctgt
    16621 ccataatctt ttttcaactt atgccgaaaa tgagcggcta aaagaaaaaa tcgatagcta
    16681 tgatgacgtt tcacagcaaa acaagaatta cgagagagaa atcgaagcgt tgaaaaaaga
    16741 acttgagttg aatcagacgc tgactagtta cgaaaaagta acggcaaatg tcattactcg
    16801 ttcgcctgac agttggcagg acatgctgat cgtagacaag ggttctaaag acggtatcga
    16861 agcaaatatg gcagtcatgt cgcaaaaagg attgattggc cgagtgatcg aggtcaatac
    16921 ggcttcgtct aaaatcgaat tactgtcatc ctctaatgaa agctccaatc attttccagt
    16981 acgggtatct tcggctaatg gcgaagcgtt tggtttgctt aaaaactatg atgaaaagct
    17041 ccatgcctta gtggtgaccc aattaactgg tgatacggat atcaaagaag gggatgttgt
    17101 ccagacatcc ggtcttggag ggaattctcc agctaacttg ccgatcggta cggttattaa
    17161 aacgaaacca gatagttatg ggctggatcg ggaagtttat gtgaaacctt atgcagaaat
    17221 gtatgacgtg tcagttgtga cgattgtcca acggctagcg gaggattagt ccatgttgaa
    17281 gaaagaaaca atgaagtact atctgccaat cgttttgttc tttttgatgt tgatagatgg
    17341 tcatttaaca agaatgctag gggagtggtc gaaaggcacc tatatgtcaa atgcccactt
    17401 tctgatattg gcattattat gttgcagtat ggcgtttgaa aaacgttatt tactgattac
    17461 cacgattgtt ctcggggcta tctatgacgc ttactatatt ggcgttatcg gtatctatgc
    17521 agtagctctc cctttaattg tatggttgat gtatgtaatg aaagacgtta tccatgtcaa
    17581 catctttact gaatttttca gtatgatcat ctttgtcacg ggttatgaat tgtttacgat
    17641 ggtggtccag ttgattttta aattagcagt agtaaataac acgtatttta ttacaaggtt
    17701 tttaggacct acactgctgt tgaacatgat tatatttgta ttattcattt ttccctttaa
    17761 gaa
//
LOCUS       DACCWK010000051        17437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCWK010000051 DACCWK010000000
VERSION     DACCWK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.051"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(131..676)
                     /locus_tag="IVW65_001646"
     CDS             complement(131..676)
                     /locus_tag="IVW65_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7900525.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(688..1380)
                     /locus_tag="IVW65_001647"
     CDS             complement(688..1380)
                     /locus_tag="IVW65_001647"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7900526.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1399..1683)
                     /locus_tag="IVW65_001648"
     CDS             complement(1399..1683)
                     /locus_tag="IVW65_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900527.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1696..2259)
                     /locus_tag="IVW65_001649"
     CDS             complement(1696..2259)
                     /locus_tag="IVW65_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7900528.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2487..3149
                     /locus_tag="IVW65_001650"
     CDS             2487..3149
                     /locus_tag="IVW65_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7900529.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3273..4469
                     /locus_tag="IVW65_001651"
     CDS             3273..4469
                     /locus_tag="IVW65_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7900530.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4570..5946)
                     /locus_tag="IVW65_001652"
     CDS             complement(4570..5946)
                     /locus_tag="IVW65_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7900531.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5983..6849)
                     /locus_tag="IVW65_001653"
     CDS             complement(5983..6849)
                     /locus_tag="IVW65_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7900532.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6938..7963)
                     /locus_tag="IVW65_001654"
     CDS             complement(6938..7963)
                     /locus_tag="IVW65_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7900533.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7981..8655)
                     /locus_tag="IVW65_001655"
     CDS             complement(7981..8655)
                     /locus_tag="IVW65_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900534.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8839..9732)
                     /locus_tag="IVW65_001656"
     CDS             complement(8839..9732)
                     /locus_tag="IVW65_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7900535.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9806..11503)
                     /locus_tag="IVW65_001657"
     CDS             complement(9806..11503)
                     /locus_tag="IVW65_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7900536.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11496..12809)
                     /locus_tag="IVW65_001658"
     CDS             complement(11496..12809)
                     /locus_tag="IVW65_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7900537.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12809..16846)
                     /locus_tag="IVW65_001659"
     CDS             complement(12809..16846)
                     /locus_tag="IVW65_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7900538.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16859..17317)
                     /locus_tag="IVW65_001660"
     CDS             complement(16859..17317)
                     /locus_tag="IVW65_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7900539.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4898 a         3904 c         3025 g         5610 t
ORIGIN      
        1 gatttattaa cgtaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa
       61 caaagtcgtt tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca
      121 ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt
      181 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
      241 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
      301 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
      361 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
      421 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
      481 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
      541 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
      601 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
      661 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
      721 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
      781 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
      841 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
      901 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
      961 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
     1021 ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc
     1081 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
     1141 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
     1201 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
     1261 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
     1321 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
     1381 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
     1441 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
     1501 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
     1561 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
     1621 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
     1681 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
     1741 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
     1801 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
     1861 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
     1921 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
     1981 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
     2041 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
     2101 agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc
     2161 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
     2221 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
     2281 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
     2341 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
     2401 atagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
     2461 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
     2521 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
     2581 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
     2641 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
     2701 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
     2761 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
     2821 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
     2881 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
     2941 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
     3001 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
     3061 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
     3121 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
     3181 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
     3241 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
     3301 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
     3361 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
     3421 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
     3481 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
     3541 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
     3601 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
     3661 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
     3721 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
     3781 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
     3841 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
     3901 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
     3961 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
     4021 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
     4081 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
     4141 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
     4201 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
     4261 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
     4321 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
     4381 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
     4441 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
     4501 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
     4561 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
     4621 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
     4681 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
     4741 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
     4801 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
     4861 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
     4921 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
     4981 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
     5041 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
     5101 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
     5161 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
     5221 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
     5281 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
     5341 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
     5401 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
     5461 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
     5521 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
     5581 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
     5641 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
     5701 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
     5761 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
     5821 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
     5881 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
     5941 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
     6001 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
     6061 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
     6121 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
     6181 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
     6241 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
     6301 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
     6361 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
     6421 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
     6481 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
     6541 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
     6601 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
     6661 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
     6721 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
     6781 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
     6841 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
     6901 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
     6961 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
     7021 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
     7081 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
     7141 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
     7201 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
     7261 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
     7321 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
     7381 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
     7441 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
     7501 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
     7561 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
     7621 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
     7681 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
     7741 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
     7801 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
     7861 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
     7921 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
     7981 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
     8041 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
     8101 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
     8161 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
     8221 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
     8281 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
     8341 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
     8401 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
     8461 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
     8521 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
     8581 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
     8641 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
     8701 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
     8761 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
     8821 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
     8881 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
     8941 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
     9001 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
     9061 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
     9121 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
     9181 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
     9241 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
     9301 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
     9361 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
     9421 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
     9481 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
     9541 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
     9601 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
     9661 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
     9721 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
     9781 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
     9841 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
     9901 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
     9961 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
    10021 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
    10081 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    10141 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    10201 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    10261 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    10321 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    10381 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    10441 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    10501 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    10561 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    10621 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    10681 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    10741 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    10801 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    10861 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    10921 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    10981 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    11041 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    11101 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    11161 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    11221 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    11281 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    11341 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    11401 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    11461 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    11521 ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt
    11581 cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga
    11641 ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt
    11701 gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt
    11761 ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg
    11821 acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca
    11881 aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc
    11941 gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat
    12001 tgatttttca agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc
    12061 tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga
    12121 cgtccatact tgaagaagta tggatctctt agataaccga catttttagg atagatatga
    12181 atctcttgca gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt
    12241 gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg
    12301 aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc
    12361 ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca
    12421 agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg
    12481 acgaattctt taggagtcat gttttcagca gctttttttt catgataata atcggtcatg
    12541 tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt
    12601 tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta
    12661 taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat
    12721 tcttctgccg acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat
    12781 tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga
    12841 caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga
    12901 ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa
    12961 gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag
    13021 gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt
    13081 ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa
    13141 taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga
    13201 ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc
    13261 ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc
    13321 cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta
    13381 gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg
    13441 gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg
    13501 caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct
    13561 catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta
    13621 ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt
    13681 ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga
    13741 tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct
    13801 cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg
    13861 ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta
    13921 tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt
    13981 ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct
    14041 tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta
    14101 catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt
    14161 cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg
    14221 tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat
    14281 tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat
    14341 catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt
    14401 cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt
    14461 catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt
    14521 ggaattgata cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc
    14581 aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga
    14641 tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg
    14701 gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct
    14761 tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca
    14821 tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt
    14881 tcactgtgca aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc
    14941 tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc
    15001 cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat
    15061 cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact
    15121 gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa
    15181 gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa
    15241 tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag
    15301 caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc
    15361 catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca
    15421 gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc
    15481 cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt
    15541 cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag
    15601 atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa
    15661 aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc
    15721 tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg
    15781 gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt
    15841 ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg
    15901 gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg
    15961 gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat
    16021 tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt
    16081 ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac
    16141 tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct
    16201 cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat
    16261 cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat
    16321 aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg
    16381 gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc
    16441 gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg
    16501 gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg
    16561 tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc gttttcaact tctccgctag
    16621 gatagatata ttcatagggg tcttgcgtag ctgttggccc aagacgctcc aaaataccgt
    16681 tgaataactg gcgttgaatg cgctggagtg cttgaccgct gggtacgatt ggttcgtcca
    16741 gcttttccat ctcattttct tcgctacgtt gaatttcagc ggaatcttct gtatcagttt
    16801 cattttcttg taattcttgg ttatctattc tttcatcaac caccacttga agaggtagtt
    16861 acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    16921 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    16981 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    17041 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    17101 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    17161 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    17221 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    17281 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    17341 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa
    17401 ttttaatttt aaatcaaatg atatcctttt tagttaa
//
LOCUS       DACCWK010000052        17097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCWK010000052 DACCWK010000000
VERSION     DACCWK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.052"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            287..694
                     /locus_tag="IVW65_001661"
     CDS             287..694
                     /locus_tag="IVW65_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900540.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVW65_001662"
     CDS             708..1109
                     /locus_tag="IVW65_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900541.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVW65_001663"
     CDS             1111..1482
                     /locus_tag="IVW65_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900542.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IVW65_001664"
     CDS             complement(1518..1820)
                     /locus_tag="IVW65_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900543.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2069..2269)
                     /locus_tag="IVW65_001665"
     CDS             complement(2069..2269)
                     /locus_tag="IVW65_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7900544.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2574..3806)
                     /locus_tag="IVW65_001666"
     CDS             complement(2574..3806)
                     /locus_tag="IVW65_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7900545.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4063..4632
                     /locus_tag="IVW65_001667"
     CDS             4063..4632
                     /locus_tag="IVW65_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7900546.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4810..5250)
                     /locus_tag="IVW65_001668"
     CDS             complement(4810..5250)
                     /locus_tag="IVW65_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7900547.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5408..6172
                     /gene="map"
                     /locus_tag="IVW65_001669"
     CDS             5408..6172
                     /gene="map"
                     /locus_tag="IVW65_001669"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7900548.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6204..7127
                     /locus_tag="IVW65_001670"
     CDS             6204..7127
                     /locus_tag="IVW65_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7900549.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7203..8342
                     /locus_tag="IVW65_001671"
     CDS             7203..8342
                     /locus_tag="IVW65_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7900550.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8335..9135
                     /locus_tag="IVW65_001672"
     CDS             8335..9135
                     /locus_tag="IVW65_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7900551.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9135..9962
                     /locus_tag="IVW65_001673"
     CDS             9135..9962
                     /locus_tag="IVW65_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7900552.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9940..10674
                     /locus_tag="IVW65_001674"
     CDS             9940..10674
                     /locus_tag="IVW65_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7900553.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10774..11640
                     /gene="rfbA"
                     /locus_tag="IVW65_001675"
     CDS             10774..11640
                     /gene="rfbA"
                     /locus_tag="IVW65_001675"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7900554.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11654..12226
                     /gene="rfbC"
                     /locus_tag="IVW65_001676"
     CDS             11654..12226
                     /gene="rfbC"
                     /locus_tag="IVW65_001676"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7900555.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12248..13276
                     /gene="rfbB"
                     /locus_tag="IVW65_001677"
     CDS             12248..13276
                     /gene="rfbB"
                     /locus_tag="IVW65_001677"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7900556.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13374..14225
                     /gene="rfbD"
                     /locus_tag="IVW65_001678"
     CDS             13374..14225
                     /gene="rfbD"
                     /locus_tag="IVW65_001678"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7900557.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14259..16292
                     /locus_tag="IVW65_001679"
     CDS             14259..16292
                     /locus_tag="IVW65_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900558.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16336..>17097
                     /locus_tag="IVW65_001680"
     CDS             16336..>17097
                     /locus_tag="IVW65_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7900559.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISY"
BASE COUNT         5377 a         2876 c         3475 g         5369 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccctcaa taaacagtcg aagagaaaat aggcaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaacaga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccgacgaaag atatatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaatgact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgcagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cagccgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3481 gcatcgggat cggcagaaac aacactgatc ctgcttgctc ctttttcaag ccattcatcc
     3541 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3601 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3661 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3721 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3781 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3841 ctattagcat gataccattt aattatgatt caaacaatga ggcctttaaa taaaaaaaag
     3901 aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt
     3961 gaagaaaatc aaaaactaat tttgcatatc aaaaaaaaca tgttacaatg aaattaatca
     4021 atggtggaag cgttataaaa aaattaagga gattaaaaaa atatggcaag aaaaaagacg
     4081 attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca
     4141 cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt
     4201 gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta tgatcattta
     4261 caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat
     4321 tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt agaagaatat
     4381 ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc
     4441 gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg
     4501 atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa
     4561 cagattgaaa agatgataca agacagtata gatgctatct tgaaattcaa agaaccaata
     4621 gaaatcgatt gaatatactc aaaagttcca caaatcagta gataacaaga gatgtgtcaa
     4681 aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc
     4741 catgaatgca atgaataaaa gagccgtaca gattgaataa atctggatgg ctcttttatt
     4801 gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc
     4861 ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt
     4921 tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc
     4981 agatccgcag acaccgtaaa tttttcccga tagatccaat tcttgcaagt cttcataaaa
     5041 gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat
     5101 atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat
     5161 atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat
     5221 gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac
     5281 aattatgaat tataccaaaa catcattgaa aagaaaataa aacctagaga cacctgggga
     5341 atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga
     5401 cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc
     5461 tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg
     5521 ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat
     5581 tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg
     5641 attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga
     5701 tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga
     5761 gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca
     5821 agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa
     5881 tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc
     5941 agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat
     6001 cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg
     6061 gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac
     6121 taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca
     6181 gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc
     6241 tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct
     6301 ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta
     6361 atattttacc gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga
     6421 ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt
     6481 caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca
     6541 atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga
     6601 tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa
     6661 ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac
     6721 ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat
     6781 ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga
     6841 tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta
     6901 tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga agttttattg
     6961 tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg
     7021 cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa
     7081 aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct
     7141 tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc
     7201 atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta ttatcactga
     7261 tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg
     7321 aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat
     7381 ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga
     7441 tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga
     7501 cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag
     7561 atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta
     7621 gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg
     7681 gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg
     7741 gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag
     7801 caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata
     7861 caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg
     7921 tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt
     7981 tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac
     8041 atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt
     8101 atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg
     8161 gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg
     8221 gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag
     8281 agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat
     8341 aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt
     8401 cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt
     8461 tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc
     8521 tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat
     8581 ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa
     8641 gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca
     8701 cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt
     8761 tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga
     8821 ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc
     8881 tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag
     8941 cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac
     9001 aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga
     9061 aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg
     9121 tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat
     9181 tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc
     9241 ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata
     9301 tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt
     9361 ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg
     9421 acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc
     9481 cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc
     9541 gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt
     9601 caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt
     9661 ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg
     9721 agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa
     9781 gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa
     9841 ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg
     9901 tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt
     9961 gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag
    10021 ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg
    10081 tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag
    10141 cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg
    10201 aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa
    10261 aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc
    10321 attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa
    10381 gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg
    10441 tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg
    10501 attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga
    10561 agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct
    10621 tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag
    10681 atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt
    10741 gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc
    10801 agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat
    10861 gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt
    10921 ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat
    10981 gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg
    11041 tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat
    11101 atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc
    11161 gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat
    11221 gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct
    11281 gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct
    11341 tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa
    11401 ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca
    11461 ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct
    11521 tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt
    11581 gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa
    11641 ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat
    11701 ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc
    11761 agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg
    11821 tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt
    11881 agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg
    11941 tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg
    12001 atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa
    12061 ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga
    12121 ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt
    12181 aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa
    12241 ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt
    12301 tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta
    12361 tgcaggaaac aaagagaact tggctggact tcctagcgat cgcgtagaat tggtcgtagg
    12421 agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca
    12481 ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac
    12541 gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca
    12601 tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca
    12661 tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata
    12721 ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt
    12781 acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt
    12841 tattcctcgc caagtaacga atatcttaag cggtattcgt ccaaaattat atggcgaagg
    12901 aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt
    12961 gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa
    13021 gaccgtgatg gaattgatct tagaaatgat gggtcaacca aaagatgcgt acgaccatgt
    13081 aaacgaccgt gcaggacacg acttgcgtta tgcaatcgat tcgacaaaat tacgggaaga
    13141 gttaggatgg aagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg
    13201 gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc
    13261 caaaaatgga caataataca gagaaaatgt cactgtatta atggaaaaac acatgtgagg
    13321 caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataat catatgtttt
    13381 tattaactgg aggaaatggg caactaggta cagaattgcg ccatttatta gatgaaaaaa
    13441 atctgcaata tgtttcaaca gatgcaaaag acttggatat cacagatgcc gagaaaacaa
    13501 tggcttacat cacagaatta aaacctgatg ttattttcca ttgtgcagct tacacagcag
    13561 tagataaagc agaagaagaa gcaaaagaat tagatgagaa gatcaatgtt gatggtacaa
    13621 gaaatgtagc acttgcagca aaagctgtga atgctcaatt cgtctatatc agtacagact
    13681 atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac
    13741 taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag
    13801 actattatat catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca
    13861 cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc
    13921 gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac
    13981 catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgaa ttcgcaaaag
    14041 aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa
    14101 aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagag ttaggatttg
    14161 tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata
    14221 agtaatttcc ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
    14281 cacgacgaaa agaaaagcca aaagcgaacc aggcagattc gaatttggtg cttgacctgt
    14341 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
    14401 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
    14461 aaactgatgt gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga
    14521 caccttttta cctctcacaa gctgaagagg actttccgtt ggtgaacgag gattattcat
    14581 taaacggaca aaacatgatc attgcgtata attctcctgc aaaggatatt acggttatcg
    14641 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
    14701 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
    14761 agagaaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
    14821 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgactg
    14881 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
    14941 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
    15001 tggcgtactt gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
    15061 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
    15121 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
    15181 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
    15241 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
    15301 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
    15361 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
    15421 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
    15481 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
    15541 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
    15601 atgcatggtc ttatttctat gcgattacgc attcgaaaat agataattat ttttcggatt
    15661 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcatttgca ttatttggct
    15721 ggagacgctt gttttacctc gcttttgccg ccttagttat cgtatctggc tttttcgtca
    15781 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
    15841 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
    15901 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
    15961 ggaaggaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
    16021 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
    16081 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
    16141 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
    16201 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
    16261 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
    16321 ttagtagaaa ggagagtgga gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
    16381 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcagggtta
    16441 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
    16501 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
    16561 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
    16621 tttggagatt tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
    16681 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
    16741 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
    16801 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
    16861 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgtacg aatcatcggt
    16921 gcagtagcag ttgttgcagc ttttctcggg atttgggtct gccaatctag aggtgcatta
    16981 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
    17041 cgtgcgccat ttattgctta cacggttacg attcttttag ctttgccgat ttcttat
//
LOCUS       DACCWK010000053        16357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCWK010000053 DACCWK010000000
VERSION     DACCWK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.053"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(38..1261)
                     /locus_tag="IVW65_001681"
     CDS             complement(38..1261)
                     /locus_tag="IVW65_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7900560.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(1292..2131)
                     /locus_tag="IVW65_001682"
     CDS             complement(1292..2131)
                     /locus_tag="IVW65_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7900561.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(2148..2828)
                     /locus_tag="IVW65_001683"
     CDS             complement(2148..2828)
                     /locus_tag="IVW65_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7900562.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(2830..3903)
                     /locus_tag="IVW65_001684"
     CDS             complement(2830..3903)
                     /locus_tag="IVW65_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7900563.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(4550..5221)
                     /locus_tag="IVW65_001685"
     CDS             complement(4550..5221)
                     /locus_tag="IVW65_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7900564.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(5308..7986)
                     /gene="mgtA"
                     /locus_tag="IVW65_001686"
     CDS             complement(5308..7986)
                     /gene="mgtA"
                     /locus_tag="IVW65_001686"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7900565.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            8645..9082
                     /gene="ndk"
                     /locus_tag="IVW65_001687"
     CDS             8645..9082
                     /gene="ndk"
                     /locus_tag="IVW65_001687"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7900566.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(9104..9970)
                     /locus_tag="IVW65_001688"
     CDS             complement(9104..9970)
                     /locus_tag="IVW65_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7900567.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(10130..12223)
                     /locus_tag="IVW65_001689"
     CDS             complement(10130..12223)
                     /locus_tag="IVW65_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7900568.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(12972..13595)
                     /locus_tag="IVW65_001690"
     CDS             complement(12972..13595)
                     /locus_tag="IVW65_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900569.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(13754..15151)
                     /locus_tag="IVW65_001691"
     CDS             complement(13754..15151)
                     /locus_tag="IVW65_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7900570.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(15183..16154)
                     /locus_tag="IVW65_001692"
     CDS             complement(15183..16154)
                     /locus_tag="IVW65_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7900571.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
BASE COUNT         4600 a         3511 c         2756 g         5490 t
ORIGIN      
        1 ttatttttat aactttcaca cacaagttta ttttttacta gaaagtagcg ccctcttgtt
       61 tttttcttgc tactatttct tgatgttctt gcttgattct ttctaatagc tttggctctt
      121 tcaacagatc aagagctgta ttggcgagta gttctgctgc aatcgcaata gaagcaaggc
      181 ctttgtcact tttagcagca gctttgaatt cttctgtgtg tccggcaata tatgtctcag
      241 agattgctac cgtaggctgg attgttggta tgacttggct gacatttcct acatcagaag
      301 aaccgagact tgttctttgt tccgtttcta tttcttcatc tggaacacct gcttcttttg
      361 catgctggaa gaataagtca tcaaaagaag gagtaacgat gacatcatca actgcatttt
      421 ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc agcacctcgt acgatatttt
      481 ccactttttc atatacttta tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc
      541 cagcagcgta atctggaatg acgttggcag ctactcctcc gtctgtaatg atcccatgga
      601 tattgacatc ttttggcaat tgcaagcgta gagcgttgat tccattaaac acttggatca
      661 atgcttccaa agcatttact cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt
      721 aaaatttgat gtcgactgga tcattcgcta aagattctgt cgttttccca taacgaaaag
      781 ctggatggac gcataacgct acatcgacat cctcaaaaaa accttctcga acaaagctgc
      841 ctttagctga tccgttctcg cccccttctt cccctggggt gccatataca cggatttctc
      901 caccagtttc atcaatgaat tgcttcacta tgctggcggc taatacggag taagtaccaa
      961 acaaattatg cccgcatgca tgaccaatac ctggtaaagc atcgaattct gccaaaaagg
     1021 caattgtggg accgggttta ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag
     1081 ctacttcttt ttctacccaa aacctttctt tttctaattg tttaatcaac gctgctgagg
     1141 aataaaactc atagttactt acttcaggat gttcgtggat atctagagca agtgcttgat
     1201 attctggtaa acgatattgg attagatcct tcaattggtt tgttgagatt gttgttgtca
     1261 taaaaaattc ctcctatgct agtcattttt cttattttct cccgaatgtt tcccatgcag
     1321 gcacgcttga tccttttgat gttttctcga ttactttgac tgtgtcttct gtttgataag
     1381 cgtctactat tttttgatac gttttattgt tttcatcttc ttttcttgca acgatgatat
     1441 tcacataagg acgtgcatgg tcatccactg gttctaagaa gatggcatca gaagcaggat
     1501 taaatccagc atctacagct acaccgctat tgatcactga aatgtctaca tctgacaaag
     1561 cacgtgctgt ttgggaagca tctaattcag aaatgtctag attttttcta ttctcggtaa
     1621 tatcagaaac agtgggcgct tgtccttttg ccttatccac tttgatcaat ccagcagttt
     1681 gcaataacag aagagctctg ccgccatttg taacatcatt agggattgct acttttgctc
     1741 cgtctttgat atcagctaca tccttcaaag tgttagaata aattcccagc ggtgcattga
     1801 cagtatttcc gatcgatact aaatcagttc cgtgttcttt attgtaattg tctaaaaaga
     1861 attgatgttg gaaagaattc aaatcaatcg atccatctgc taaagccaca tttggttgat
     1921 tgtaatctgt aaatttcacg tattccaaat taatgttctc ctttttcaaa cggtctttca
     1981 cgttgtccca tacatctgta tcttctccaa taatccctag ttttacgata gtttcttttt
     2041 tgtcagttgt atctgctttt ccttggctac accctgccaa ccaaataatt gctacaaatg
     2101 taatgactac aaaacgaata atttttttca ttttttctcc tccactttca atgtgtaatt
     2161 tttttaatca gttgattact gataaattga cttaaaaaaa ctaagattag aatcaataat
     2221 gtagccacaa tcgttacatc tgtttggaat cgattatacc ctctcgatat agcaagattc
     2281 cctaatccgc ctgcacctac tgctccagcc atggtagtca gtccgatcac gttgatgata
     2341 gtcaacgccg agacccgaat gataccaggc aaaccttcta acaaatacac acgtaaaatg
     2401 ataccgattg gactggttcc catcgcttct gccgcttcga tcaccccagg atcaacttct
     2461 agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa ctccaaaaat caatggcact
     2521 aaagctgctt tcgcaccaat cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga
     2581 gaaagcaaga taatgaaagg gatggaacga atgatattga tcagcttatc aagtatttca
     2641 aatagcagac ggttagctag taatccatta gggcgtgtca ctactaacgt cacacctaag
     2701 atgattccaa tcatcccgcc aatgaatgct gtccaaaaag acatatatag tgtttgcttc
     2761 gtcgcttcga caaattcatc cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa
     2821 tcagtcattt tatcttcctc cttcgatcac tttgatacga ctatctaaat cgtttttttg
     2881 taaaatattt atttttacac cagatttgat caaataatcc aatgcttttt tccgctgttt
     2941 ggtttctcca gctaaagtaa cgatcaaact ccctaacggc gtttcttgta ataactctac
     3001 attgccatat aaaatattcg ctgttacttg atatttgctg tacagatgag cgattagagg
     3061 ttcgttcgtc tgatttccaa tataacttaa ttccaccagc catttattgg cgatctgatc
     3121 ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg tcaagatgag tagctgttcg
     3181 aataaaatct ttggtcaatt cttcttcagg attgctgaag atctggatgc tgctaccttt
     3241 ttcaacgatt cttccatctt ccataaccgc taccttgttg cagatttctt tgacaacttg
     3301 catctcatga gtgatcagga cgattgttaa acctaactgg cgattcaatt tttttaacaa
     3361 tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg gttgcttcat cacataataa
     3421 taccttaggt tcattcgcca aagctcgtgc gattgctact cgttgttttt gtccccccga
     3481 cagttgttta ggataagcag atgctttttc ttccaaaccg acaagttcca gtagttcatt
     3541 cactttttga cgacgttcct gtttcgattt tccagaatac tttaaagaaa aatcaacgtt
     3601 gtcaaaaata gttcggcttt ccattagatt gaaatgctga aagatcatac cgatggcctt
     3661 tctttctttt cgcaattctt tgggagagag ctgactcaga ttcgtctgat tgacaatgac
     3721 ttctcctgct gttggtttct gcagcaaatt catcacacga actaacgtac ttttccctgc
     3781 acctgagtaa ccgacgattc cataaacatc gcctttttca atcgttaaat ctacagaatc
     3841 tactgcctgt atctcttttt ctttttgttt gaaacataca gaaacatctc gcaattcaat
     3901 cattaaaatt cctccttatc taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct
     3961 tgaggacgct tttttgcgtg ttaccacctc atgttcaagc tgtgttcaca cacaactcct
     4021 cttcaagtac gatcatactc tagcaagata tcggtcgcag ccgtcatcgc cttacatcta
     4081 tgctcgggat gaattgctcg atggccattt tccaatagtc gttccttgct tcttctcagc
     4141 taccgaagct ctctgtcaaa ggttcttcta tcgtacttac catttcaatg caaataatat
     4201 tcaaaaaaaa agtcctttta ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg
     4261 taccaccttt tttcggaagt ttccttccct caagcccaac atctcaaaaa gagagattgg
     4321 cgttcgtttt aacggtcgaa cagttccgaa tttgcctact tatcgacaaa ttattctcca
     4381 aggccatttt cagcttttct tacttgactc ttctcaccag ccagagcctc tctgcaaagt
     4441 ttccaaactt actttccttt tcaacgaaat cgatattttg ttgttgggat taatctacat
     4501 gaggaaaact gaaaagtcaa gtacgaaatt caaattattt tttctatatt tatttttctg
     4561 tttttttatt taacatttca ataacagttc gaagatcttt tccttccgca agaagtttca
     4621 atttttcctt ttcttcgacg gtataatttc ggacaaaact ggaatcaggt atcaaaacaa
     4681 cgcctaatcc tgctcgtgaa gcagctgttg ctcctactaa agaatcttcc actgcaaagg
     4741 ctgaactagc ttctaatcct gttcgttcca aagctgtcag ataaggaatc ggtgaaggct
     4801 tgttctccga cacttcgtct ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt
     4861 gatccaagat atctgttgct cttttcgtaa cagtggtcgt agcaatgcct agtttcatat
     4921 gacgttcttt taagtctgcc aatatttctt ttgcatatgg ttttaagtgg atggctccgc
     4981 ttgtcagctg ttgataaaaa tattcttcac gcttcgctcg gacttcctgt actttttcag
     5041 ggctacctgc tgctttcact aaaatatcaa aactttgttt ccagtttttt ccgccccaag
     5101 cgtcaacgat ttcttttggg atatggatat caaattgctg aaatccccaa agccagccat
     5161 cacgatacgt tttttctgtg tcaactaaca aaccatctaa atcaaatatt gcacctttca
     5221 tctatacatt aatcctttcc aaaacaatac acatagttta gcacgactgg tccaaaaaga
     5281 acagaagcaa agaaccagcc ttctcgttta taagagttct tggaatcgac ggatatagat
     5341 tttcttcaca gctgtcacaa gcgccagata agcgatgact gtagcaaata accataacca
     5401 gtaattgcca ggaagtggca tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac
     5461 agaaccgaca ataattccta atgtagtgat aacaaacatg gcaaatgttg cgttgctttg
     5521 gataaatggt actttcggtg tacgcagtgc atggagcact aaggtttgtg tccatagtga
     5581 ttcgacaaac catcctgcat ggaataatgc gatgaaaatc actcgctgtg aactatccaa
     5641 tgtatggaaa cttccgccca ctacagcagg acaaataaca aaatacatca acaagtaagt
     5701 agtaatatca aatatcgaac ttgttggacc aaagtaaacc atgaattccc caatactaga
     5761 tgcattccat ttttttggct cgtccaaata ttctttatcc atccgatccc atggcaagga
     5821 aatgcaggaa acatcataga tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa
     5881 aaatggcagg aacgtacttg caatcagcac ggaaaacata ttgccaaaat tagaacttgc
     5941 tgtagccttg atatatttca tgatattccc aaaagtttct cgtccagaaa gaaggcctct
     6001 ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct gcagattctt tcgcaatatc
     6061 cacagctgta tctacagaaa taccgatatc agccactttc attgcaggag cgtcattgat
     6121 accatctcct aaaaatccga cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc
     6181 tttttgctga ggattcaatt ttacgaatac ttcatggtct tcagctactt gtgctaattc
     6241 atgatcactc atttccgaga ttttctcacc ggtaaccaat tcattgatct ctagacctac
     6301 ttgacggcac actgaacgtg taactaattc gttgtcccca gtcagaactt ttacagctac
     6361 accatgttct ttcagagctt tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa
     6421 agcaagataa ccaatcaaaa ccatgtctga ctcatctttg actgaaaatt ctccaacgac
     6481 gcttggattg gtcttttgtg cgacaccaat cactcgtaag ccatcttcat taagtgactg
     6541 tacttttgca agtacggctt cttttcgttc ttttgttaat ggtgtaacag taccgccaag
     6601 atcaataaat ttagaaatgt tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt
     6661 tttaccagct ggatcagcaa ccacaacact catacgacga cgttcaaaat caaaaggaat
     6721 ttcatccact ttttgataat tgatcttatc tgtatctaaa gtttctgttg cttcatcaat
     6781 gatagccaca tctaacaaat ttttcagccc tgtttgatag tagctattta gatacgcatg
     6841 gcgcaaaaca cgatcatctt catttccttc acaatccata tgatattcaa gaatgatttt
     6901 atcttgtgtc aatgtccccg ttttatccgt acataacaca tcgattgcac caaagttctg
     6961 gatggcattc aaatttttga tcactgttcc ttttttggcc atcacacttg ctcctttgac
     7021 caaattcgtc gtaacgatca ttggaagcat ttcaggtgtt agaccaactg ctacagatag
     7081 tccaaaaaga aaagcttcta gccaatcccc ttttgtaagc ccattgatga cgataactgc
     7141 tggagccatc aatgacataa atttgatcag tagcatggat gttttatgga tgcctaattc
     7201 aaagcttgat tgaattggtt tttcagatag cgtttctgct acatgaccaa atagtgtttc
     7261 atttccaaca gaaacaatga ttccttccgc acttccactg atgacattac ttcccataaa
     7321 gacaagattt tcgtaacttg tttcgctttg ctgatcgtct tcacatactt tcgcatgctt
     7381 ttctactgga tagctttccc ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga
     7441 caataaacgc atatccgctg gtatcatatc ccctgctgaa agccgaacaa gatctccaca
     7501 gacgatttct tccataggaa tctctgtgta ctttccatta cgttttacag ctgccgttac
     7561 tttcacaagt gatttcagtt tttcagccgc ttgattggaa cgtacggact gaatcaacgt
     7621 cattgtcccg ctgacgataa ccatagtgaa gataatggct acaccgaaaa gatctttatc
     7681 tccaggagct gctaatacat attccgtgat gaatgaaata attcctaatg cgatcaacac
     7741 taatgtaaat ggcgtgatgt atgctttcaa tatttctaca atgaatggtg tttttttgcc
     7801 atatgaaatc gcattttcac cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa
     7861 tccatttgct gatgcatgga atagttcaaa taattcttgt ttcgtttttc cagcaaaagc
     7921 tgcatagatt ttttgtgtat ctttcttttt ttccatctca atctctcctt tgtgtccaaa
     7981 agacagctgt tctcgctgct tttggtcttt agctattcat cggatcgata gagaaaaaac
     8041 tgaaaggggc tgagaacaga tagataatag ccctggcctt tcctctagat ccttgttttt
     8101 cgaatgactg tcatccattt gttctcatct cctttactaa gtaattttaa ttcatgactt
     8161 ccaatcattt ttcgaatagt tatttttcca attagaccac ctccttttta tttttcaccg
     8221 atggggattt gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa
     8281 gcatattaaa aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa
     8341 agcagatacc tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac
     8401 ccattggcgt ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta
     8461 acaaatctta ttcggttttc cttcgttaat ataacaacta aaaaagagga tgtcaaatca
     8521 attttccatg tttttcatac tttttttaaa atacacacgt tatattcctg tctcacgcgg
     8581 ttttatttgt ctcattttcc acctcccgct ataatagaaa tatagtagga aacgaggtgc
     8641 tgttatggaa agaacacttg ttattatcaa gccagacggc gtgagacgcc atttagtcgg
     8701 aagcatcatc caacgttttg aagcaaaggg actggccatt gctgaaatga agtttgaaac
     8761 tatgactcct gaattagcaa aagaacatta tcagcacttg actgagcgca gtttttttga
     8821 tgaactgatc gattacatga cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga
     8881 agtgatcgat atcgtccgta agatggttgg agcaacaaaa gctgccgatg ctgttccagg
     8941 tacgatccga ggagactacg cgctgccggg gactgaaaac atcattcacg cttctgattc
     9001 ccgtgatgct gcggtaaaag agatcgcccg ctttttcccg ccattcgata taaacaaacc
     9061 gtctgaaaaa gcggcgaagt gatacaaatt ctaaactgct ggtctagttg ctgggttcag
     9121 caatggccag cagttttttc tctaaggacc tccgttgatt ctttcctaac atatattcga
     9181 gcactgtgaa gaattcttgg tagtctttcg tcttttcttc ccctaaagca gcagaaatca
     9241 atctttgata ataatctgcc atcaatgttt catagatcac tgtacttctt tctttatgtg
     9301 ctaagctccg tttcatatct tcattcacac tacgtgcctt tttcaaccga ccattttcta
     9361 tataaaacga agtcaggttc attaaaaaag taaagagata ctgctcatga ttgttcaact
     9421 gtgctaatcg tcgcattctt tttttgattt tcaaataatt gttctcaatg aaccgatcac
     9481 tgaatatcca aggaaaatca gctagaatag atagttcaaa ttgaccatag tctggtatct
     9541 gcatcaagta ttcttcaatg attcggcatt cataacgtaa aaattcaaat tctgtctcag
     9601 atacttctgt tttttcgacc atgtgaagat tcaaaaccaa ataatacaga tcttctgttt
     9661 tcttaaaatg cctacggtta ttgctgatct ctttataagg atcaaagctg tcttcttgta
     9721 cccgatctag cattcgtctt tttttcttta actttattgg ttcatccagc tggtttcgca
     9781 tccattcata ttctgcaagt gtcatgttca ttcgctctaa aaatgatctc aatgttacaa
     9841 atgacatttt tttcactgaa tttgtctcaa tcccgactag agaagtctta aaacagattc
     9901 cttccgctaa ttgggactgt gtcaacttct tgttgactcg aagtttcttg atcaattcac
     9961 catcatacat aatagttctc ctttttaatt taaatttttc cacattcaat aaatagatac
    10021 gttaaaaagg aaaaatacac gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa
    10081 ccttcaagca ttagaatgtt gtctaactac tgaaggttgg tttgattgtt tattgattat
    10141 ttttttgctg ttcaacgtct gttcttgtta ctggatataa gttggttgtt gttttattac
    10201 cattttttga aaagacactt gtcgaatgtt cgcctttttg cttttcgatt gcttcattac
    10261 gttccgcttc atttttatag tcatattttt tcggatcaat cgtctcaaat ccgtttggca
    10321 cataaaaccg caagagattt tcctgattca gcttatctga aagacttaac gcagtcttga
    10381 ctttttcctg atctttggag atttcctcat cgattcctgc tgctttcgta tcgatgattt
    10441 ctcctgtctg attattgtaa gcttttcctc ctaggacggt gtatttgggt gtcacaaagt
    10501 tcccatttcg aaaagcaact acttgatcat gctgagaaga taataagtct gtaccaaaat
    10561 gaagataatc tttatcatcg attcccaata gatgtagtaa tgtcggcaaa acatcgattt
    10621 ctccaccgta ttcatgattg actgtccctt tagtataacc aggcatatgg atcatcaatg
    10681 gcactcgttg catctgtgcg ttgtcaaatt catcccatgt atctggatct ttaccaagag
    10741 cgcttgctaa atctttatta tctgtattcg agatcccaaa atgatccccg taaatcacaa
    10801 agattgaatt ttggtaaata ccagattttt tcaaatacgt aaaaaattgt tccaatgatt
    10861 gatccagata atgtgccgtt cgaacataat tatctactgt gctattgcca gtattcaaag
    10921 atggaaagtc gaaattcttg tcatccgtat aataaggcgt gtggttggtt acggaaagaa
    10981 atttcgtgta aaacggctgt tgtagatgct caagatattg ggcagattct ctgaataaat
    11041 ctttatctaa tatcccataa cctaacgttt cgtcggattc atcaaaataa gatcgatcaa
    11101 agaaattgtc ataacctaag tttttatata catgatctct attccaaaaa ctagctacat
    11161 tcccatggaa aacagcgctt gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt
    11221 gaaagacatt gtctgaccca agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc
    11281 cagtttccaa catattttca gcatcacttg tctttccctg tcctacttca tgaaagaaat
    11341 tgtcaaagct gatcgttgct tgattctgga aaatactatt taaaaacggt gtgacttctt
    11401 gtccgtctac cttcatatta atcaaaaact gctggaaact ttctaaatgg agaacgatca
    11461 catttttccc tttagcgatc ccaaatgttt cgggatttgg ctctgcataa tgtttctttg
    11521 tataatctaa aacttcatca atcccattac tgcttgcatg cgcgcgaaca ctgctagtca
    11581 ttcctgtttt gattccgtca taaatagtat acgcatcgat tcctagatat ttgacaatat
    11641 atgaacggtc aaatgttcgc tgcagcaatt gtggacggtt ggcttcgctc agcgctagat
    11701 ttcctgaaaa aataagccct gaaagacagg taacagcgat agccatcggt ttgccgacag
    11761 ggttgctttt gataggtatt ttcttgacct tcaaccaaac cagtagccca atgaacacag
    11821 cgatatccag ccagtagatg acatcatggg gcttcatcaa agaaaaggag cttcctgaca
    11881 atccttgcga cactttcgaa aagcctaata cagatttgac tgtaatgaaa tctgtgaatt
    11941 cccgataaaa aataatattc aagtacaaca aaagcgtatt taaaatatca atgatcaaaa
    12001 gaatagctaa cgctggcttt ggctttttaa tatacaaaga aaggccaaaa aacaataagg
    12061 tagtcgcaat tgggttgatc cacaagataa agtactggat cgtaccttcc acacctaatg
    12121 aaaaatctgc atagtaggca atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa
    12181 cgattgctaa acgattttgg cacaattttt taagttgtgt cacgtatatc acttctctct
    12241 attcacattt tttcttattt tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc
    12301 ttaagatttt tttattcttt ctaaagatta ttccgtccac ttttcacagc ataaaaccgc
    12361 tgcgatgtaa gcgattaaac taatgaagag ataaaagtct tgtggtgacc gcttcactcc
    12421 acggtattca aaagccagtt tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc
    12481 ttgtcgcagc tccttatttc ttgatgcagg acagcttttt cacaacttct tccttaaacg
    12541 gtgttctcct gccaactcct cggccacaag tcacaatatt tataaaacat gagaagctgt
    12601 tttttgaaat tgttccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca
    12661 cggtgttcaa tcagctgacc ctcggtatta gattatgtca ggacgctaat tttacaacct
    12721 cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
    12781 ataaggagct attttttgaa attgctcctt gtggctcaga gttaaacggc aggctcaaga
    12841 cctcttgata cacggtgttc aatcagctga ccttcggtat tagctcaaaa aacggcaaaa
    12901 tatgaggaac tattttgccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca
    12961 acctctgttt ttcattgtcc ataggattga tattgtcctt gtgatgactc attcgtaata
    13021 attgaggctt gattttcttc acttcccgtt atcgtttgtc cactgccaga atctgaagga
    13081 cttgttcctg tcgactcgtt tttttgagaa gagacagttg ttttcttgac ttgttcattt
    13141 gtctttttcc attccgctaa acgatcggcg accacttttt tgtctacttt ttcagaataa
    13201 gcggtgatca cttttccatt tttcaaacgg gctactttgt cgccatcatg agtcaaaagc
    13261 aaaacggtca aatgcttgtc tttaaattga tattgtctga gagaaatcgc actaagtacg
    13321 tcatcgatat taataaattg attttcttct tttccttttg gcaaataaat gtccaattcg
    13381 cctttttcgt aattggcagt tattttatac tccgcccacc aataagttga gaattctttt
    13441 ttgaaattat ctactgattc tttgcttgct acgatttttt ctgtttcttt ctgagcaaga
    13501 tctgctgtca tttttcctga aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat
    13561 aaccaaaaga gaagaaacgg tctttttttc ctcatgcttt cctccactcc tatttaattt
    13621 tgactattct attatataca aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt
    13681 aaataattga acttcttttt cacaaaatgg taagaatgga gaatttcacg catttgctct
    13741 caactattct ttcttaagcc tcaaatccgt ttttatctga tacgtctttg aaccaatgac
    13801 ccgatttttt gattgttctt tttccatctt gtgctaagtc tactgaaata aagccatatc
    13861 gattcttata tgcattggtc cacgaccagt tatccataca tgtccacata tgatagccta
    13921 aacaattggc accttcttga atggcttgat ggacatattt cagatgagat tggacaaatt
    13981 cgatccgata atcatcgtgg atcatgccat cttctttgat aaatcgtgct tcgttttcta
    14041 cacccatacc gttttcagaa ataaagcagt cgatatttcc ataatgatct cgtacattga
    14101 tcaaaatatc atagattcct tgttcataga tctcccatcc gcgataagga ttcattttac
    14161 gaccaggcat ttcataattg tcgaaataat cctcaggcat tggaccgttt ttagtatcga
    14221 ttggtgtttc ttttgctttt acccggcgtg gctggtaata attgatacct aaaagatcta
    14281 ctgtattttc tttgataacc gtcaaatcct ctgcatggat cacaggcatg agatcaagtt
    14341 cttttactat ctcaatcaat tcacttggaa actcgccttt tacagctgga tcaaggaatg
    14401 aacgattgaa gaacgcatct gctatttgcg ctgctttaac atcttcaggg tttgattcgt
    14461 ctcttggata gcttggtgtc agattcaaga tgataccaat aacaccacct tgctccattt
    14521 catggtaaga tttgatggct aacgctgaag ccaacgcttc atgatagcca acttggatcg
    14581 cttttttcat atctgactct tctggataat gccatcgata taaataacct gcttcgactg
    14641 gaacgattgg ttcattatga gtaaaccatt tttttacttt cgaaccaaac aattcaaaac
    14701 aacttttggc atatttttca taggcttcaa ccgtttctcg gttcaaccat ccgccttttt
    14761 cttgcagata catcggcata tcgaaatgat acagattcac aaagggttcg ataccgtttt
    14821 caatcaactc atcaaaataa cgatgataga aacgaacggc ttcttcgttg attgttccag
    14881 attcatcagg tatcaaacgg ctccattgga tagatgttcg aaatgaatta tgcccagtct
    14941 gtttcatcaa ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc
    15001 ctacttgatc aaagaacttt tgcggttctt ttttgtacca ataatcaaaa atgttctctc
    15061 cttttccatc gcctgaaatc cttccttctg tttgtgggcc acttgctgca gaaccccacc
    15121 aaaaatttgc tggaaattga tattttttca tcaaagcacc tctttttctt ttttagttcg
    15181 ttttagttga ttccatgtat gatattttcc atcacaatca ctttttcatt ttcttcctct
    15241 tcttttttga ttcgttttaa cagctgttct gctgctcgtt ccccgatttt atctgttggt
    15301 ggcgtgatcg tgattttcgg aaggatactc agatcgtttt tatctaaata atcatagtga
    15361 ctgaacgcaa tatccttccc gattctgaca ccttcttgat tgaaacattc gatggcacca
    15421 atcgacatat tgtaattgct gacaaaaata gcagttgccc ctttttctaa taatgctttt
    15481 gccccgtaat agccgctttt tctagaatag tctcctgtaa agacctctaa ttggatcgat
    15541 ggatcatttt ggaccgcttc tttggctcct gctaaacgtt ctcttgctac gtagtctgtt
    15601 tgagtggcag caataaatcc gattttttta tgtccttgtt caccatgatg agcgatcact
    15661 tttgctacac tttctcgatc attcactaat attgtatcaa tacctgctgg tccttctgga
    15721 caattcaatg cgataactgg aatactcatt tttgtgagca tttcaaatcc agaccactct
    15781 tcttcggata agaagacaat cagcccatcg atcacatgct tcatcaggta ttcaattttt
    15841 tgttcaattt tttctggctg atcggcaaac ccgcttaata ataagctata cccattttct
    15901 tcagctactc tttcgatccc tgagacaact tccgacccaa aacggctgga aatcccgttt
    15961 atcaaaagtc caatagagaa gcttcgatta ttcttcaacc ctttagcaat gatgttttct
    16021 ttatagtcca acgctgaaat cgcttctgct atttttttca tgttcttttc cttcagttgc
    16081 tgaccattca agtaacggga aactgttccc actgaaacac cagccatctt agccacatca
    16141 cggatcgatg ccatacattc tcactcctgc cagttacatt tgatttctct ttcttgttgt
    16201 tcgattatga tgaccgcatt ctttgcatag tgttgcttgt catcaatatc catataacta
    16261 aagtcttcag tagagcaact tcctctcact attaattata cttaaacaag tatttgaacc
    16321 ggttcatttt taatatagca tttttttaaa agattgc
//
LOCUS       DACCWK010000054        16074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCWK010000054 DACCWK010000000
VERSION     DACCWK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.054"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            136..453
                     /locus_tag="IVW65_001693"
     CDS             136..453
                     /locus_tag="IVW65_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900572.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(656..1021)
                     /locus_tag="IVW65_001694"
     CDS             complement(656..1021)
                     /locus_tag="IVW65_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900573.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1229..1711
                     /locus_tag="IVW65_001695"
     CDS             1229..1711
                     /locus_tag="IVW65_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900574.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1908..2798
                     /gene="pstS"
                     /locus_tag="IVW65_001696"
     CDS             1908..2798
                     /gene="pstS"
                     /locus_tag="IVW65_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7900575.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWEIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3123..5597
                     /locus_tag="IVW65_001697"
     CDS             3123..5597
                     /locus_tag="IVW65_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7900576.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5700..7436)
                     /locus_tag="IVW65_001698"
     CDS             complement(5700..7436)
                     /locus_tag="IVW65_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7900577.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7433..8137)
                     /locus_tag="IVW65_001699"
     CDS             complement(7433..8137)
                     /locus_tag="IVW65_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900578.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8268..8770
                     /locus_tag="IVW65_001700"
                     /pseudo
     CDS             8268..8770
                     /locus_tag="IVW65_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
     gene            8730..9068
                     /locus_tag="IVW65_001701"
     CDS             8730..9068
                     /locus_tag="IVW65_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7900579.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9089..10507
                     /gene="ffh"
                     /locus_tag="IVW65_001702"
     CDS             9089..10507
                     /gene="ffh"
                     /locus_tag="IVW65_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7900580.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10619..11197
                     /locus_tag="IVW65_001703"
     CDS             10619..11197
                     /locus_tag="IVW65_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7900581.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11201..11722
                     /locus_tag="IVW65_001704"
     CDS             11201..11722
                     /locus_tag="IVW65_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7900582.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11801..12355)
                     /locus_tag="IVW65_001705"
     CDS             complement(11801..12355)
                     /locus_tag="IVW65_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7900583.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12608..12883
                     /gene="rpsP"
                     /locus_tag="IVW65_001706"
     CDS             12608..12883
                     /gene="rpsP"
                     /locus_tag="IVW65_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7900584.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12895..13146
                     /locus_tag="IVW65_001707"
     CDS             12895..13146
                     /locus_tag="IVW65_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7900585.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13270..14061
                     /locus_tag="IVW65_001708"
     CDS             13270..14061
                     /locus_tag="IVW65_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900586.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNDKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14231..14752
                     /gene="rimM"
                     /locus_tag="IVW65_001709"
     CDS             14231..14752
                     /gene="rimM"
                     /locus_tag="IVW65_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7900587.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14742..15503
                     /gene="trmD"
                     /locus_tag="IVW65_001710"
     CDS             14742..15503
                     /gene="trmD"
                     /locus_tag="IVW65_001710"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7900588.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15639..15986
                     /gene="rplS"
                     /locus_tag="IVW65_001711"
     CDS             15639..15986
                     /gene="rplS"
                     /locus_tag="IVW65_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7900589.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         5372 a         2858 c         3284 g         4560 t
ORIGIN      
        1 gatggtaact atgcactgta acgataatgc tgctaaaata tctttttacg aaaaacatca
       61 caaggaaaaa cttaactatg gtattatcta aacataattt tttcggtatt taatatttaa
      121 ttaggaggta atattttgct tttttatagt aattttatct taatagtagc gatattatta
      181 cttctgaata tatggatttt tgataggagt agaaatgcaa gtataggttt tagaactaaa
      241 cgaagtctgt cttctaaaaa aaattgggta tactcacaaa ctatttttta tggaggaatt
      301 gttttaatta gtttactttc ttcaacactt tattctttga atattataga tgtttccacc
      361 tcaaattcaa tatctattat tggaatcatt attgctgcaa ttattacaca actgttttta
      421 gtgtttggag agaagaaaag aagtaaaaaa tagaaaaact agtgatagtt ttggtgatag
      481 tgatattaaa aatcagaaaa gatagaaaaa gcaggagata gataaaatgc tgatatttca
      541 gcatttttaa gagtataaaa gagaaaaaaa gtaataaatg tatacatgat ataagaaaaa
      601 catatatgta taaaaagaag aaagtagctt ctctttgtac atatatggtt tgtttttatg
      661 gatgccgagc ttttttataa gataaggcta ccgcccataa cgacccaagg atttgtggaa
      721 ctaagtaaat acctgcaaga agcaagatca gctgggtcgg ttttaaagga tgatagaagt
      781 aacgccattc agtaaaattg gcgcaaaata aataggaagt gacagtagat ataatattgg
      841 caatgaataa agatataata cgcctgttcc tttgaaaata atatccagca aaaagagata
      901 aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg aaaatcaagc agtaaggcga
      961 aatgcacata aggaacaaaa gataaaagca acggaaggtt tttccagcaa gtttttatca
     1021 tctttcatca ccacctaaca agtgttatta ttttgttagt tttattgtaa attgaatcct
     1081 gcaataatca attacttttt cattatttct agattaaatg aataaactct catgccatta
     1141 tctctaaaaa aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg
     1201 agagaaaaag tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta
     1261 tttatttttt tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt
     1321 acaaggtgcg gagatattag gcctgatcca aggaagcgga tttgtggttt ctaggaattt
     1381 tccagctttg actgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct
     1441 gatcaatcac tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctat
     1501 gttttctttg ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa
     1561 ggattcagct tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat
     1621 tgcctcattt attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga
     1681 acgatctgta tctataaaaa ataactattg atggtggatc atcagtaaaa aagcatatga
     1741 gctgcgtaaa atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa
     1801 acgtttacat gatttttaca taacttaact atatttttac agcaaccata cgagggattt
     1861 acagtcgact gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat
     1921 tgtttactct aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata
     1981 cagatggctc tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg
     2041 cggtaggttc gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag
     2101 aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag
     2161 tcgaagctgg agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag
     2221 ttgatgcttc aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca
     2281 ataaagatac agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa
     2341 aaatcactaa ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg
     2401 caaacggcag cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg
     2461 tgcagtccca agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag
     2521 gagcaatcag ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg
     2581 acggtgtaga accaaaagaa gagaatgtag cagataacag ctgggaaatc tggtcatatg
     2641 aacatatgta cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga
     2701 tgacagaaga aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga
     2761 tggaagtaga acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg
     2821 tagtcgttac tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa
     2881 ttgtgaaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga
     2941 ttgaacaccg tttattcgaa aaaaagagct acgacataga actgtcgtag cttttttttg
     3001 ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg
     3061 ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc
     3121 acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag
     3181 ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg
     3241 aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc
     3301 aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt
     3361 tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc atagccaata
     3421 caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt
     3481 taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata gatatagaag
     3541 accttgtaat agatgacatt gttattttag agactggaca tcaaattcca gcagatgcag
     3601 aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa
     3661 taaccaaaga aataggcgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt
     3721 acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca
     3781 agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat
     3841 ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta
     3901 atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac
     3961 ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa
     4021 agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc
     4081 tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa aaagtgatcg
     4141 tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact
     4201 atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa
     4261 aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca
     4321 gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc
     4381 aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg
     4441 tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat
     4501 taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact
     4561 tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca
     4621 atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca tctaaattat
     4681 cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag
     4741 aacaaaagaa aatcttcgtt caattgctaa agcgtcgtaa caatacagta gctatgacag
     4801 gtgatggcgt gaatgatatt ctagccatga aagaagcaga ctgcagtata gcgatggctt
     4861 ccggaaatga agccgccatg caggcctcac aagtggtctt acttgattct gacttttcac
     4921 gcatgccaga agttgttgca gaaggacggc gcgttgtgaa caatattgag cgatcagcca
     4981 gcttgttttt agtgaagaat attttctcct ttttgctctc actgttttca gtgatctttg
     5041 ctctgaccta tcctttagaa ccatcacaaa tcacattgat tagtttgttt actatcggac
     5101 ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg aatcagaggg aaattcatca
     5161 tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga tatgatcgta gtcggtgcgt
     5221 tggtgatctg cggggtgact ttgaatctaa ataaaacaga tgtttctaca gcatctacca
     5281 tgcttttgat tgcagtaggg ttcctcgttc tctataaaat ctgttcaccg ctgaataaat
     5341 tcagaagtca gatcattctt ttctgtgcta gcggtaactt tttttcggtc atctttttgc
     5401 ataagctgtt ttcaatcacc ggtatctcag ctgtatcact tttgctgttg agcattctat
     5461 ttttctcggc agattcgatt tttaggcatt tgaccacatt ggtagaatat ttgtttgaac
     5521 gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagt taaaaaattt ctatcgatat
     5581 ttaaacgaga aaattgacga aaagtatctc aaaaaagagt aaaaaagaac aaccactcgt
     5641 gtatctttcg aagcaggatg agtcatccaa ctttaaaacg cacgagcagt tgctcttttt
     5701 tacattttcg gaatggtaac ggtgaaagtc gaacctgttc caagatgact gtctacagta
     5761 atcgtaccat gaaggagttg cacgtagtct tttacaatcg ctaaccctaa tccggtcccg
     5821 cctgaatgcc ggcttcgcgc cttgtctacg cggtaaaaac gctcaaagat ccgttcctga
     5881 tcctcaatgt cgatacctat accaaagtct tggacagaaa acgatagatc atctgttgcc
     5941 tgatagcgga taataatctc gctgtcgctt tttgaatatt ggacagcatt ttcaatcaga
     6001 tttttgataa ttggataaag gatatctgtt ttagtagtaa atggttcgtt ctttccaatc
     6061 aatcgaattg tcaaccgttt cttttcgatg attggctgat aattttgaac gatttgctgg
     6121 aaataaggtt ctgtataaat tgtctggatc tcgtaagaat tctcgccatc ttttgacagc
     6181 tggatgattt cccgaatcag tttatctaac cggatcgcat ctttttgcat gatctcaaga
     6241 aaagaagtca gagtttgcgg gtcatctttt gcaccatcca gtaaagtctc ggtaaaacca
     6301 atcagtgaag tcacaggagt ttttaattcg tgagagacgt tgccaacgaa atccttttgt
     6361 agcttttcta aacgtcgcac tttcgttaag tcatacgcca cccctagaat ctgtccggta
     6421 ccttctgaat tagtaaagta gcgcaaactc aagtctaatg tctgccgacc ttctgtaagg
     6481 gtgatctcct gatggatcag cggatgctca ggagtcactt gatgaatcag ctgaatgagt
     6541 tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt gatagcttac cacgtttaaa
     6601 tgtagctgca tctttggatt aagcagtagt aaacgagaat cagcatcatc aatcaggaat
     6661 acgccaatca tcagttcgtt cagtaacgta tataattgct cttctgtggt agtatacgcc
     6721 cgatacatct tactcatttg ttcgctcaac gcattgatcg tctgatacaa ttcttcccat
     6781 tgatcgggtg tttgcatgat tacttccgcc tgattaggat tttgcaccat tttttttagt
     6841 acgggcagca cggttttcaa tggctggttt ttttgataga tcagataata gatcattcct
     6901 gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc aacggcgaaa actctcagtt
     6961 ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa tggcttctaa ctggccactt
     7021 tttttgactg gtaatgcgac atataaaaga tccccattca aagtagtact tttgcgcaaa
     7081 gcgctaccta ggttcccacc attcaatacg gctttgattt cgggacggga gtttcgctgt
     7141 tcattcaatg tttcattgtt tgtgtcaaat aagatctttc cagtagcatc cattaaagta
     7201 atccgttcgt tcgactgatg gacgaattct tccaaagcag ttttgttttg cgctgcttgg
     7261 aggttttcga catctaattg ccggatcagc agttgtgctt tttttgtcag gtagctttct
     7321 tgctgttcca cgatctgctg ttgaaaataa tgggaaatca attgccagcc accaaaaaac
     7381 aaactaacta aaagaataag aaagaagaga cgttcataaa tttttttcca tttcatcgtt
     7441 tgggttcctg aaacttatag ccaaatcctc taatagtcac caagtacttt gggtgttttg
     7501 gatctcgctc gattttttct cgcaaatgac taacgtgtac atctacgatt cggctttgtc
     7561 ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc tcggtcgatc acacggtctt
     7621 ttcgtttcat gaaataaaca agtaattcaa attctttcgg tgttagttca atcagatggt
     7681 cgtctacggt cacttgataa ttggtcaaat cagcagtgat ttgtccaatc gatagatatt
     7741 caggctcagc ttcatctttt gattgcttac gaggttcaat tcgtctgaat attgctttca
     7801 tacgggctaa tacttcgcga ggactaaatg gtttggtcaa atagtcgtca gcacctattt
     7861 ctaggccaat gatccgatcc acttggtcat ctttagccgt cagcatcaaa atcggtgttt
     7921 catttttttc ttgacgcagc cgctgagtga tctccatgcc atccatcgtt ggaagcatca
     7981 catctaaaat gatgaaatca aatgattgtt ctaatgctaa ttctaatcct tcgcctccat
     8041 ctgtggcact ggttaccttg tagccttctt tttccaaatt gaacgtcaag agcgttacaa
     8101 tagaaggttc atcatcaaca actagtattt ttttcattca aatctccttt aaataggttt
     8161 cattctgttt atattttagc atagaatcag tacaaagggc tattttacaa aagaaataaa
     8221 tggtataata ggaaggcact ttgctgaata gaaaggaaga gcagtcaatg attggaatca
     8281 gtgcatgttt aggcggtctg gcctgtcgct atgatggaca gaaaaaagaa gtaccagaat
     8341 tgatggaact agttgaaact ggagaagctg tcatggtctg tccagaagtg atcggcggat
     8401 taccaattcc tagagatccc gctgaaatca ttggtggaaa tggattcgat gtgtggaaag
     8461 atcaagcaaa agtatggacg acaagcggcg aagatgtaac ggaagcgtac aaaaatggcg
     8521 cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga tctagtcatc atgaaggaaa
     8581 aaagcccttc atgcggaacc cattcgattt atgacgggag tttttccgga ataaaaaaaa
     8641 tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc tctctgatga
     8701 agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat cgaatgaacg
     8761 cgctctttga attttattcg accttattga cagaaaaaca aatgaactac atggaattat
     8821 attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta agccgacaag
     8881 cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa aaaaaactcc
     8941 atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact gcatatatta
     9001 cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt caagcaatcg
     9061 aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg aacgtctcca
     9121 gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg taaaagaaat
     9181 gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag tagtcaaaga
     9241 ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa gtttatcacc
     9301 tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg gttctgaaac
     9361 agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg gtcttcaagg
     9421 ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa atgaaaatgc
     9481 tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc aattgaaagt
     9541 attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca gcccggtaga
     9601 gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg tattgatcga
     9661 tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac aaatcaaaga
     9721 attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc aagatgccgt
     9781 caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca tcactaaact
     9841 tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag gcgcaccaat
     9901 caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc cagatcgtat
     9961 ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag cgcagcaaga
    10021 ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt cattcgattt
    10081 caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg aagatctaat
    10141 caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg tagatccaaa
    10201 agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa gagcaaatcc
    10261 tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa acagtgttgt
    10321 tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc agcaaatgtc
    10381 caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg tcaaaggaaa
    10441 actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa agaaaaaacg
    10501 gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc cggagctttt
    10561 ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg tggcacagat
    10621 gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata ctgtctgggg
    10681 tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata ttaatcaagc
    10741 cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag catatgatcg
    10801 attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac taatggaaaa
    10861 caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg ccaaagcagt
    10921 cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt tttctaatgg
    10981 aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa gtcctctttc
    11041 agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa gcacaacaat
    11101 ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt gtttccgcaa
    11161 agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa tcatatttta
    11221 tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc aatggctttt
    11281 tgacactcca acaggtaagc aaaccactta tgaagctttt tatgaaacaa tcgatacgac
    11341 catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg aagcaattta
    11401 tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc cagatgctga
    11461 ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag aaggaaaaga
    11521 tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc aaatgattga
    11581 tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt tattccaaaa
    11641 aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg aatttatcga
    11701 attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat atgcagaaat
    11761 gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt aatttgttag
    11821 aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct tcaacgatat
    11881 taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt tgctgcaagg
    11941 ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca aagccgccga
    12001 tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca ctgtgtccat
    12061 aaggccttga gcctacatct aaagcatttt tcaatactgc tggcacagaa cggttgtatt
    12121 caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa cgagtccatt
    12181 cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga tcatcgattt
    12241 ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct gctacttttt
    12301 tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt gtcatacgat
    12361 ttttcctcct tcaaactatc tataacaatc ttacaaaaaa taagtaactg gagcaaataa
    12421 aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac aaaaaagaag
    12481 tctaaaatct tctatttcct aaaaaaaatc gaatttaatc aagctgtaaa gaaaaaaagc
    12541 tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat ggaggtggaa
    12601 ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga gtccgtttta
    12661 ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg aaactgtagg
    12721 tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact tagttcttga
    12781 ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt caaaagaagg
    12841 cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc ggatatgaaa
    12901 gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc tgatcaagtc
    12961 aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt ggctcctgaa
    13021 gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg cacaatcgtt
    13081 tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga tgataaggaa
    13141 aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgctt ttttattaat
    13201 tgaagaaatg attatttggc aaaataagca agaaagcgta tccttatagg aggagggagt
    13261 gaacagccaa tgagaaagaa aatgttattt attccatttg ctctattgtt aagcgcttgt
    13321 agttcggtta ctcaagaatc agacaataca catctatcaa gcagtatatc tgaaactgtt
    13381 accagtccag aaaaaagtag taccaaaacg gctaccacca ctgaacaaac aatggctacc
    13441 tcaaataatg ataaaaagac tgctttagac cagttgaaag aacaacagcc aaatgttccc
    13501 atgcctctag atgtaccagt ctcttcaggc tatctcaata tcgcagcgac acatacaaag
    13561 caaggttatt ctattttata ttacagaaca gatcgtcccc tcgggttgaa tgctgacgaa
    13621 ttgaatcaag aaacgcctat tgcaacctat ctatatcaat atgggtttgc ttctagtcaa
    13681 gaaacaatcc aagttttgca gccctttgag atcgacacaa atggtcagca agtggactta
    13741 ggttctcgaa tcacagggta tcagcaaggt gcagcaggtt caagttttct tgaatggcaa
    13801 gaaggaaact ggcgtattcg aatccgtggg aataatatag aaggccaaga tcccttacta
    13861 ttagctaaag aaatcgtagc ttatttggaa gaaaacagtc taccagcacc tgaacaattt
    13921 ggtaaaatta ctgttgatat gggtgatacg acgaatcgag ccgtagaagt aagctggcaa
    13981 gaaccgaaaa atgcctatac gatcactcat caagatccta tgtctgcgtt gaagatggca
    14041 gtttctatga agaggttgtg aaatcagcgc gctgatctga gcccatcatt taactccaag
    14101 tcacaggaag taatcaaaaa tagcacgccg catttttgca taattttggc ttaatacctt
    14161 aaaagaaaac tagaattgcc tcttcacaca tgctaaaata aaacagacaa aaaagaaatg
    14221 gggatataag ttgactgaat acttgaatgt aggaaaaatc gtaaacacac aaggtatcaa
    14281 aggcgaagtc cgtgttatct caacaacgga tttcccagaa gaacgttaca aaaaaggaac
    14341 ggtgttaaca ctattccaag acggaaaagc acctgtggaa ttaacagtca aaagtcatcg
    14401 caaacataaa aattttgact tattgagttt tgaaggacat ccttctatca atgatgtgga
    14461 aaaatatcgg gatggcatat taaaagtgtc aaaagacaac tctgtagatc tagctgaaaa
    14521 tgaattctat taccatgaga tcatcggtct gcatgtatta gaagatggaa ctgagttagg
    14581 taaagtaaaa gaaatcttgt caccaggtgc caatgatgtt tgggtcgttc aacgtccgaa
    14641 aaaagctgat atattgcttc catatatcgc ttctgtcgtc aaagaagtcg atttagaagc
    14701 tggagtggtc cgtgtagaaa ttccagaagg actgattgac gatgaagatt gatgtattga
    14761 ctttatttcc aagaatgttt gaaggtccta tgggggaatc catcatcggt aaggctgtag
    14821 ataaaggatt attagatatc aatatctcta acttccgcga ttattcagat aacaaacatc
    14881 agacagtaga tgattatcca tacggcggtg gagcaggtat gctgctcaaa gtccagccca
    14941 tctatgataa catccaagcc attgaaaaag ctgcaccgga tgtcaaaaaa agagtaattc
    15001 ttcttgaccc agctggtaaa cgattcgatc agaaaatggc agaagaattt tcacaagaag
    15061 aacacttagt attcatttgt ggtcattatg agggatatga tgaacgtatc cgcacattag
    15121 tgacagatga agtttcctta ggagattatg tccttactgg gggagaatta ggtgcgatgg
    15181 tcatgatcga tgcaacagtt cggcttttgc cagatgtgtt agggaatcag acttcagctc
    15241 aaacggattc ttattcaacc ggtttattgg aacatcccca atatactcgc ccggcagaat
    15301 ataaagggat gaaagtacca gaagtattga caaatggaaa tcataaattg attgaagaat
    15361 ggcaattaaa agaatcacta agaaggacct atcaacgtcg gcctgatctt ttggaaacac
    15421 ttgaatggac acctcaaatg acgaaatttt tagaagaaat caagaaagaa gaacaagaaa
    15481 aaacagcaga tgaagccaag tgactttttc taaaatattc tttacagtag gttgataata
    15541 tgttagtatt ttatggtgag tgcaaaagca cttaactatt acgatattcc gctgtgagaa
    15601 aagctcataa gaatatttgg aataaggaga agaaaaccat gaatccatta atcgaagaat
    15661 taacaaaaga acaattacgt tctgacatcc cagcgttccg ccctggtgac actgtgcgtg
    15721 ttcacgcaaa agttgttgaa ggtactcgtg aacgtatcca gttatttgaa ggtgttgtta
    15781 tcaaacgccg cggtgctgga atcagcgaaa cttatacagt acgtaaagtt tctaacggag
    15841 tgggcgttga acgtacattc ccattgcata caccacgtgt tgcaaaaatc gaagtagttc
    15901 gctacggtaa agtacgtcgt gcaaaattgt actacctacg tgcattacac ggaaaagctg
    15961 ctcgtatcaa agaaatccgt cgttaatcat tgttatcaga ctgagaaaga acccttggta
    16021 ttcaagggtt cttttttctt agcatgtaat ttgaacaccc tgatccagtt tttt
//
LOCUS       DACCWK010000055        16060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCWK010000055 DACCWK010000000
VERSION     DACCWK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.055"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            645..1031
                     /locus_tag="IVW65_001712"
     CDS             645..1031
                     /locus_tag="IVW65_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7900590.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIELAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1125..1316
                     /locus_tag="IVW65_001713"
     CDS             1125..1316
                     /locus_tag="IVW65_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7900591.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1830..2162
                     /locus_tag="IVW65_001714"
     CDS             1830..2162
                     /locus_tag="IVW65_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7900592.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2458..2613
                     /locus_tag="IVW65_001715"
     CDS             2458..2613
                     /locus_tag="IVW65_001715"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7900593.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2889..4229
                     /locus_tag="IVW65_001716"
                     /pseudo
     CDS             2889..4229
                     /locus_tag="IVW65_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4470..6086)
                     /locus_tag="IVW65_001717"
     CDS             complement(4470..6086)
                     /locus_tag="IVW65_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7900594.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6204..6422
                     /locus_tag="IVW65_001718"
     CDS             6204..6422
                     /locus_tag="IVW65_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7900595.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6617..7078
                     /locus_tag="IVW65_001719"
     CDS             6617..7078
                     /locus_tag="IVW65_001719"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7900596.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7271..7510)
                     /locus_tag="IVW65_001720"
     CDS             complement(7271..7510)
                     /locus_tag="IVW65_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900597.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7534..9057)
                     /locus_tag="IVW65_001721"
     CDS             complement(7534..9057)
                     /locus_tag="IVW65_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7900598.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9075..9197)
                     /locus_tag="IVW65_001722"
     CDS             complement(9075..9197)
                     /locus_tag="IVW65_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7900599.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9227..10210)
                     /locus_tag="IVW65_001723"
     CDS             complement(9227..10210)
                     /locus_tag="IVW65_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7900600.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10234..10383)
                     /gene="rpmG"
                     /locus_tag="IVW65_001724"
     CDS             complement(10234..10383)
                     /gene="rpmG"
                     /locus_tag="IVW65_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7900601.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10404..10781)
                     /locus_tag="IVW65_001725"
     CDS             complement(10404..10781)
                     /locus_tag="IVW65_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900602.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10813..10992)
                     /gene="rpmF"
                     /locus_tag="IVW65_001726"
     CDS             complement(10813..10992)
                     /gene="rpmF"
                     /locus_tag="IVW65_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7900603.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11007..11276)
                     /gene="rpsN"
                     /locus_tag="IVW65_001727"
     CDS             complement(11007..11276)
                     /gene="rpsN"
                     /locus_tag="IVW65_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7900604.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11799..13571)
                     /locus_tag="IVW65_001728"
     CDS             complement(11799..13571)
                     /locus_tag="IVW65_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900605.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13525..15279)
                     /locus_tag="IVW65_001729"
     CDS             complement(13525..15279)
                     /locus_tag="IVW65_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900606.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15389..15958)
                     /locus_tag="IVW65_001730"
     CDS             complement(15389..15958)
                     /locus_tag="IVW65_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7900607.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         5202 a         3062 c         2427 g         5369 t
ORIGIN      
        1 tatctaaaat actattcctc tgtcaaaata agctaactta cttcattata aaatatccac
       61 ttatcgcttt catgtatatt ccaccgtaaa aagtacattt tatcaccgag aactcatcat
      121 ttttcatcat gatgaatact atttacatat atctagttca aagacaataa ttattatgat
      181 ccgttattat ctaagcacta tacagataat aggaattttt cgtttctaaa tattccaatt
      241 tgggaatcca ataaaaattt tcgttcaaat acgtgtctat tcgaactctt ttctagttgt
      301 taattatgaa gggggaagaa ataaaaaata caatctatac acgaataaga atattatcca
      361 aaaggaggtt ttagaaaaga tttattatac gtaaaaagct caagacattt tcttagccat
      421 aaggacatgt ttgatattta agaagatata tgaattttct tttctaagta taaagataac
      481 tatccattat caactaaaaa aacatttttg aaaataaaca tcagcagtcc tatcaatgca
      541 tgtgagctta tagaagttat tcgcagcgca gcataattgc attagcttct tttcgtgttt
      601 tttgcctctt ttccctttaa atcttaatgg aggaggtgtg caatatgtca gataacgacg
      661 aacgcgtaaa tgcccagttg attcgattct ttgaaaaggt cattttgaac accgctaata
      721 cttattataa aaaacaagaa gaaattcaaa aacatgaaca acttgatcaa ctcccaccat
      781 atttgtttac taaagatact ataaaattta aacaaccgat taatgtttta aatgtgactc
      841 tccttgttga agatatagaa ttagcaaaaa tccttccttt gttgaaagaa cctgaacaaa
      901 tttttcttat cgaaaaattt atctttgata aaactgataa agaaattgga aaggttctgg
      961 ggattacaag acagggtgcg acaaatttaa aacatcgact ttacaaaaaa attcaaaaaa
     1021 tgctggttta atatctgcat aataactaat tttgtatgct tctttgctcg ttctcctcct
     1081 tcccaaagaa atagattata agtccatggg aagaaggtag atttatgaat tctttaaaag
     1141 aagatttcat tttagcaaag gctggaaatg aagaagctgt tgaggcaatt ctcaaaagat
     1201 ttagctcttt gattcataag caatcctgga gaactggaaa atatgatcaa gattgttatc
     1261 aagaatgtat gcttgcaata tatctagcta tctcgaaatt cgaaattaaa gaataactca
     1321 actaagggag tctgtgacat aagtaaaaag atagtttcaa atttcgaaca ataaatttga
     1381 aactatcttc tttataatta taaagtatcg attttacagc gttctataat gattcataga
     1441 atctaaaacg aatgatttta attatcaacg ttttttaatt cgtttttagc ttaaaaataa
     1501 cttttgtcac agtctcactc ctaaaattac tttatttacg tatgaagtgg gcaggaggtc
     1561 gaatccagaa ctgggctatg gtacttcaac gaggacttct cctctagaat tgaaaagcat
     1621 gcgatttacc ttccgtaacg acggtagatt tattcaacta tgtgtccaat caaaatttgt
     1681 ctgatttcaa aaatagtcaa gagaattttg gttgttaaaa actcttatct atcaacttcc
     1741 acagtttagt tgacaaaccc tgatactaag ttgggctatg atacactttt cttgactatc
     1801 agttacgtta ccggaaggaa tgaactctat tgagaaaaac atatgatcgt aaatttaaac
     1861 taaagatttc acaggatatc cttgagaaaa aagtaactac caagaaaatt gctgaagaat
     1921 acaatatctc tcgtccaaca atttcccgat gggtttcaga atatcgtcga tacgggaaaa
     1981 atgcttttgc tggacaagga aaaagattac ctgataaagc agatttctac attcttgaac
     2041 aggaaaataa acgactaaaa gaggaagacg agtgttctac gaacacaaag atcgatacgg
     2101 atctccaggg atcactcaaa aactttacga tgaaggaatc gaaatcaata aacgtgttgt
     2161 agctgttctt atgcgtgaaa tgaatttatg cacaagagga tttcatcgac gcaaatcttc
     2221 tcatggtaga gaaaaaacaa tcaaagaaat ggtaaaaaaa tgttgaatcg ataatttgaa
     2281 cagaaccaaa ttgatgctgt ttgggtgatg gatattactt acattccatg taatgacggt
     2341 cgcctctatt tgtccaccta tattgactta gcaactcgta ttctacgtta ctatatggta
     2401 gattcacaca tgaaaaaaga gattgtcgtc cgtccattac aaatttatta aggagaattg
     2461 cctacggtaa ttcattccga tagaggtagc cagcatcggt cttttattta tcaagagctg
     2521 ttgaacgacc accacatcac acattcagtg agccaaactg ggactcctgt tgacaatgca
     2581 gtaattgaag cgtttcatcg atcaatcaaa tgagaattaa tcgactccaa caaatataaa
     2641 agtaaaatag agatgaaggt gttaatccaa gattatttag aagactacta cccaaacaaa
     2701 agaatccata caaaatttat gatgactcct tgacaatttg aagcgaagaa aataagggat
     2761 ctataatatc tagtgttggt gacgccttct gttgccaata ctttttcttt tttctaataa
     2821 acgcaaacag acaccttaaa atcctttaga ataaagtcga cgaaaaccat tcaaaggagt
     2881 gtttcaagat gtcctattca gagtttacca aagagttact agatattcta gacctaaatc
     2941 ttatctttca tgaagatgcc tttcgcaaag agcgaatcaa tgacgaaaca tgttttgttt
     3001 ttgatggcac attgacctat caaccggagg aatgctttca tcgccactat caaaatgaac
     3061 aaacgatcat taaatagggc tggaaaaaag tatccatctt attgaatgat gtcagtaact
     3121 acaagaccat tctgcggatt aacaaacagc gcttcaaatg taaacactgt ggaaagactt
     3181 ttttagcaga agattcagtt agtgatcgtc gctgctccat tgctcgcaga gtcaaacaag
     3241 ccattcttga gctattaagc gaacctcttt ctatgtcttt gatcgcacgt atgaagcaca
     3301 tttctccgac cactgtcatt cgaatacttc gcagccttcg gcctaaaacc atttctttga
     3361 gccagctgtt accagaagtg gtgtgtttcg atgaattcaa atcagtgaaa aatgtctcgg
     3421 gagccatgag ctttgtcatg atggacggca agacccatca actgattgat attgtggaaa
     3481 atcggcaact caatccttta cgcgactatt tcttgcgcta ccctataaaa gtccgtgaac
     3541 aagtgcgtct agtggtttca gatttctata caccctatcg cacattggtg aaagagtgtt
     3601 ttccgaatgc ccgaatcgtc gccgatcgct ttcatattag ccaacacatt ggtcgggctt
     3661 ttaccaacca ccgaattcaa gtaatgaaga cctttaaaaa aggggatcgt cggtcgaagc
     3721 acttaaagaa atactggaaa ctacttcaaa agaatgcgtg ggaattgaaa ggtcagcatc
     3781 gctactggcg tccgtcattt cgagatcatc tgacagaagc agagatagtc gatcggctat
     3841 tatcttatga tgattcctta aagcgaggct acgaagtcta ccaggatttt ctttccgtta
     3901 tcagaagaca agatgttcct gaattcgtcg cgctcctaaa agaagactac aaggagttgc
     3961 cagagcacta ccaaccagtg ttcacgacct tcaagaaata ccaaacagaa atcaaacgag
     4021 cgttgcgcgt tccctattcc aacggcccta tagaatgttt gaataaccat atcaaagtac
     4081 taaaacgaat tgcctatggc tatagaaact ttcaaaacta ccgagaacga atctttttat
     4141 atcgaggaaa atattttaag aaaactaaga atactaccca actgactaaa gccagaacaa
     4201 caacacggct agacaagata gctgtctagc cgtgtgagta tctcgattat tctattgaat
     4261 ttcttcacca acactcattg acgaagagcc aaaataagta atctaaccga ctgttaacta
     4321 aaccattgca ggtcccgtta aagatacttg tgcctgttgt caccgcttct ttttcttgtc
     4381 cacaagcgct taacacactt aataaagccc ctgctattat taataataat atctttttct
     4441 tattaaaaac ctcctcatct atttccagat tagtaaatgt agtaatctgt gctttctttt
     4501 ttctcaatac taataactaa cttatggggt aaacaaatac tcgtttgacc gggttttgag
     4561 atccatccag ttctaaccgc gatttgatca ggactattat cttctttaac tcgaattctt
     4621 ccatctttaa tctcaacaat attatattgt cccttcgcag gatgataatt cacgagttta
     4681 tgattgatct tatccaaatc gaagcgatcc atttccttgc catcaattcg aacgatggca
     4741 tatttgatag catcttcagt tgtttcattc tccttttcta tagagaatac aaataaggga
     4801 ataaaagagg taataatcaa aatgccaata attatgacat cgcctatctt gacaccagct
     4861 tttcttaaaa atttcattaa cgatatctgg ctaaacatat tagacttttt ccatggaaat
     4921 ggtgaaattt ttcggaaatt atctgcatta agagaatcag ttaaacaacc ctcaagttgt
     4981 tccttaatac attgttgatc catcttcata ccaataaaaa ctagtttatt aatgcgttca
     5041 ccaaacaacg gatgccaggc ttccttcaat tcgggatttt tttgtataat catttgacac
     5101 tcaattttag gtaaactagc tacccaataa tttaatgatt caatactttt atagctaccc
     5161 gatgtttcaa agatcgtcga aaaatcacta tgttgggcaa accaaagaaa cccttttgct
     5221 cttgtaatat tgccaggaaa atttttatcc gtaagatggg caaatttttg tgcgtcaagg
     5281 agtaaccgat ttcgatagat aaaacttgag ataccatatt cttctgtttc aggaatatga
     5341 ttttgatagc caagctctaa ctctttgaac cagcccactg aataggaagt ctcatctata
     5401 ttaaaaagat ttgtatttag aattttcgat aagtctactt cacctttaat tgtcctaatt
     5461 attttggcat tggactgtaa cttagcaatt agaccgttaa ttttgtaaat cgtcttggtt
     5521 gacactaagt cactattatt taaaagtaat atgttacaac actcgaattg atcaattaac
     5581 aagccacgaa tatcctgttc ttcttcaact acattacctt cagcttttct ttcctgaatc
     5641 ttttcacccg tttcgaaatt atcccaaaaa cgttttacat ccactactat aataatggca
     5701 ccaatctcta atcgttgggt caaatcaatg tctaaagggt ttttagctaa tataattgct
     5761 tgaataattg gaattggttt gctgattcct gaagcttcaa taacaatttg attcaagtca
     5821 gaaaattgca ctaattgatc tagttcaaca atcaaatctt cgcgtaatgt acaacaaatg
     5881 catccattcg gcatcgaaac aagtttttct ccagttcttt taaaaccttt cttgtcgact
     5941 aataccccat caacatttac ttcccctatg tcatttacaa tgacagcaat ttcctttccg
     6001 tgatcaccgt acagaatatg atttagaagt atcgtttttc ctgaaccaag atagccagtt
     6061 aagattgtaa taggtatttt tttcataggg aacctctttc tataccttga atttacactt
     6121 taagtgcaac acataaaaat gagtcataat cgatatccaa ctagtataag tttgtcgaga
     6181 acactcctag aaggaaaata attatgagcc agtatcgtca tcttactata ctcgaacgag
     6241 aaagaatctt tctgcttcac gaagaaggat tttctattag tataattggc aacgacattc
     6301 agcatagccc gtttactatt ttacatgaac tgagaagaaa caagaaaaaa actattcacc
     6361 ttccaaagcg gaaaaaaatt atgcttatcg aagaaaaaaa tgcggacgtt atcacattct
     6421 aaatgatccg tgagtttggt caattgttcg tcatttattt gtcgattttc agtggtcgcc
     6481 agaggaaatc tcgaatcgtt tgaagcatga acatatggac ttttcaatta gttttatcac
     6541 gatctaacgt gcaatttgca atgatctttt cgatacagaa cccctctcac acggcaataa
     6601 aggcctgatc cgttcattgc accatcgagg aaaaacaaga catacaaaaa atcatgtcga
     6661 gtctcgtgga aagatttgcc ttactcattc gatccatggc cgtccaaaat ccgcagatga
     6721 tagaagtgat tttggtcatt ggaaagccga tattgtagct ggcaaaactg gtggtacttg
     6781 tttagtgact ttgacagaca gaaaatcatg cttcctttta gcaggaaaga aggtccccag
     6841 aaagaaagcc gcttatgtga gcgagaaaat gattgaactt ttaagcagta ttccaaatga
     6901 ggcggtcaaa acgatcactc cagatagagg aaaagaattc tccaatcatt tcgcagttac
     6961 agaagcattg aataatatgc ccttttattt tccagatcct catgcccttg gcaacaatga
     7021 acaaattaaa atacgaatag actcattcga gaatacttgc caaaatcgaa agagatagct
     7081 acggtagata actcaacgat aaatcagatg attgaaaaac tgaataacag acctagaaag
     7141 tgtttagatt ggagaacacc ttatgaagtc ttctttgata aagtgttgcg cttgatttga
     7201 caattcaaga cataataaaa agcagatcaa atccaaataa gggaaaaatg atctactctg
     7261 gtattatcaa ttaattggcc aagctattat gccgttttgc taaacgtcta cttcttccag
     7321 tattgacctt gataaaacaa caatcaggat cccgttttaa tactctatga actgtgtcaa
     7381 tactaacatg taaagttgca gcaatatcag ctaatgtact cccatttaat ctcatttgct
     7441 ttgcaaatcg ataacgccga taagcatcaa tattcttgga atccatagac aataatactt
     7501 ttcttaccat gtatttcctc caaaaattga attttaatga gccatcattt cttgagcaat
     7561 ttcatgttta gacaatgaag ctggatagaa agtaggccaa ttatgcaatt cattatagag
     7621 tttttcttga ctttctccac caaagaaaat atggaaatgc tctgccttac ttggtgcaat
     7681 tccatgatca ctaaattgaa tatatttaaa acgaccagca cctgcatcat cagtttcaaa
     7741 attaaaacga acaccacggt ttcctttttc ataattcaaa attttgtatc caacatattt
     7801 gtatgtgtat tgatgatgtt caccgttgac tacaaaatca atggtgttgt ctttaatgtt
     7861 gatagtatca atatctgtct tatagccagt agtataataa tctttgtact cggcagctgt
     7921 catgtcttta ttgatttttg ctttgtaatc gaaaacttca tccaaaattc catcttttaa
     7981 caacggataa acagattgcc attcgcctgt ataatcactc aattcgcgat ccttcacatc
     8041 ggcatcttta aagtacccat tagcaactgt cttttcatcc ttcgcttctt tttctggaac
     8101 tacctcttca ccagcaacca tagttgtttt ttctaacgct tttagattgt cttccattac
     8161 tgaaacataa tcttcaccat tatccatttg ttcttttgta agactttcaa gaggattcag
     8221 tacttccaat tttatcccag cttcgttggc caaagttttt gcaattttat cattagcatt
     8281 cttttcaaag taaatataat tgatcttatt ttttttaaca tactctttta attcagctaa
     8341 tcgaccagaa gatggctctt cttcaggact tagtccagca ataggaactt gtttcaatcc
     8401 ataatccaaa gctaaatatc caaaggctgc gtgttgcgtc acaaaacttt tttgtttcgc
     8461 ttctttaaga ctagtggtat agtcagcatc caaccgtttc agtttagtca aatacttttc
     8521 agcatttgtc tcaaatactt ttgctttttt cggatacaat ttaactaact gatccttgat
     8581 atttgaaact tccttaattg ccattttggg tgaaacccac gtatgaggat ctagttcatg
     8641 atggtgatct tcttctccgt gatcatgatc ttcttcttca ctacctggca ataaaatcat
     8701 gtcttttgtt ccttctacta cattaggccc agctttcttc caactttctt tagcttttgg
     8761 tacccatgat tccatgtttt cgttatgata tacaaaaaca tcagcatcat gagcttttgc
     8821 catatcctta gctgagggct catagtcatg aggttctgat cccgctggaa ccattaaatc
     8881 cacgttcgct tcatcgccga cgatgttttt ggtgaaatca tacattggat agaatgttgt
     8941 tacaatttct aattttccat ctttatcatt cgatactttt gcatcattag tgctggagca
     9001 agctcccaat aatcctagag ctgctatccc aataagtcct acactgattt tcttcattct
     9061 ttttccttcc ttaattatgc taatttcttc attttatttc gtttatgttc tttgataatt
     9121 tttaatgccc tttttctagt tttaatattc gggcttttaa gtcgtcgata tgcactagat
     9181 atatccgttt tctccattag aaattacccc caatcactac ttatcattat ttgctttccg
     9241 ccagtaattt atcaaaactc tgtttgatcc tttcttcatc caacattttt ccgattagaa
     9301 taatagtggt ttggtctttt tgtcgagtgg tgtattgaaa attaacctgt tcgtttacct
     9361 cttgcatggc aactataaaa tcctgctctt gtaaagcaat caatccttta aaacgataca
     9421 ggctcccttg gttggttaaa atcagccagt caatccattg gagtaaaaaa tcttttttta
     9481 tgtcttgatc tgaagttaag agaattgatt taaactcatg tacttgatgg tgttgatgat
     9541 agtatttgcc attatagtga ttttctgatg aatcactttc aaaaactaag ggatgattaa
     9601 attttttcaa attataaaaa tccttttttt gaatcgtttc actatgtgtg aacattcgaa
     9661 tatcggataa agggttaata cttttgattt ctctctgtaa tttttttagt gcagataaat
     9721 tcaagttttg ttttctagac acaaaaatgc gatctgccat gacaagttgc ttaagggctt
     9781 gtggttctct ttcaataagt tcaaaactgt cactatcaat aacagtcaat aaactatcga
     9841 tatatactct tcctcttagt tgaggcgctg ttaaaagtgt ttgaatgatt ggttggggat
     9901 ctgctatacc tgtagtttca atgatgattt gtgaaattgg ttgttgatgt tcttctattg
     9961 tttccagtaa agaaatcaat attgctaata aatcactgcg aagactgcaa cacatacatc
    10021 cattatttag ttgtaacacc cgttcctcgc tatgaactaa taatttgtga tcaatcccaa
    10081 cttcaccaaa ctcattttca attataataa tttcttccct ggggtagggt gagacttgca
    10141 aggcttgatt gattagcgtt gtttttccag cccctaaaaa cccagaaacg attgtaactg
    10201 gtatgcccat tttgtcacct ttttaccact tagctatttc acttctttaa aaattgcccg
    10261 tctgcgcaat tttggcgaat acttttttaa ttctaaccgt tcagggttat tccgtttatt
    10321 tttgctggtg agatacagac gttctccagt ttcgacacat tccaaaataa tattttgacg
    10381 cataaatctt cctccttcaa tacttatcga tcttcaagat atgcttgaat ttgttgtgca
    10441 tcttcaaaag ttaactcgcc agattccaat tgtgaaacta aattttcttt catatcaaca
    10501 ggtatctccc aataaccatc gatacattga aataactcct cgtcatactc ttcaaaaatt
    10561 ggatagattc ttaaaaaatc atcaaatgat aagccttggt cctcatagta atctttgact
    10621 aatccatttt caattgcctg ttgggtcggt aaacccgttt cagtaataat tcctctctct
    10681 accatcgaac gataaaaagt gcatttatct agcgattgct gggtcgtttc aagataatac
    10741 tttaatcctt cttcaactaa aggtgggtaa ataatttcca ttttgattcc ctcctaaaag
    10801 ttatatatta aactattttg aagctccttt aattactttc tttccattat agtacccttt
    10861 taaagataca tgatggctac gtctataatc accattcgct tcgtcaaatg aaatttctgg
    10921 ttttactaca gtttgatgag ttcttcttaa ccgtttcttc gccttagatg tttttcttgc
    10981 tggtactgcc atgatatttc ctccttttac caacttgctt tttttactcc aggaatcaac
    11041 ccttgatggg caagttctct aaacttaata cgggacatac cgaactttcg catataccct
    11101 cttggacgac catccgtctg atcacgaagc ctcagacgat tcggatttga atctcttggc
    11161 aatttagcga gagcggtgcg atctcctgcg gcttttaatt cctgacgttt atctgcatac
    11221 ttttcaatca tcgccatttg tttctttgct ttagcaattt tcgacttttt tgccatatac
    11281 ctttatactc cttttatttt gatttagaca cctcaaatcg taatcgttac gttttgacac
    11341 tgatacttta ccaacaagag ttgctcttgt caacaataaa taacatgaaa taaaactcat
    11401 atgaatagat aactatttga aaacgaattt atcggtacat agagataatc aaatcattta
    11461 aaagaatatg cagtttaggc tctgaaaaat aagaattcac ttttattagt ccaatcaaac
    11521 acaaataccg ttttaagtag cttttcctct acttaaaacg gcaatatgaa agaaactata
    11581 actttttatt caatctttta tgtttaactg gtttatcgcc aattttttaa aactttgatt
    11641 attccttata ttataagaat agacattaag tgaactaatt caatcatact gattctttcg
    11701 tgtattcaaa attagatgct gtttaataaa attattcttt ttgaaaaaga attataacta
    11761 tatcccaaaa attgtaggtt aatttaaagc ctatttcatc atcgtgattg agcttctttt
    11821 tcatagacga atttctcaga tagtttagtt ttctttatga gatttttgta tatttctgac
    11881 cttcgataca actcattatg agtaccttga tcaactagtt ttccttgatc catcactaca
    11941 attttattta ccttttcaat agacttcaag cgatgcgata taattagtac tgtcttatct
    12001 tgtattaaac gatttaaact ttcctgaata cgtttttcat tttccgcatc taaactggca
    12061 gtaatttcat ctaaaattaa aattggcgca tttttcaaaa aagcccgtgc tatggatagg
    12121 cgttgtcgct ctcctccaga aagtgaactt ccgttttctc cgaccaatgt atgataaccg
    12181 tcaggtagtt tttcaataaa gtcttcgcag ttagctaaac gagctgcttt tttcacttcc
    12241 tcatctgacg cttgactatt tccaatacgt atattttcca aaatacttgt attaaacaaa
    12301 gttacctctt gaaaaacaat agcgattttg ctaaacaaag aagctggaga tacacgttta
    12361 atatcatacc catcaattaa aatacagcct tcatcataat catacagtct agacactaat
    12421 ttcaagatac tagttttccc ggaaccacta gcacctacta acgcagtcac ttctccttgt
    12481 ttagccgtaa aagagatatg atctaaaatt ggggtattgt tatcatatga gaaagagaca
    12541 tctcgcaact caatgtcaaa agatttcaga ggactatctg atccctcttg gatactggtt
    12601 tccttcatag ctctaattcg ctgtatttta ggagctaaat aaaatatttc aagcactgct
    12661 tctttcatag aatcgaagga atcctttatt tttattgcgg ctagtaagta accaacgacg
    12721 tagagtatag tcacttcacc cgttaacagt aagtgaacac ctactagaat gactactgct
    12781 aaactaacat atgagaaaat agaggataag gccatgactg aaaaagtact taattctacc
    12841 tttaaatgaa tcctttcact ctcttccatt tttttgaaaa gtctgtcttg aactttttta
    12901 gatagattaa agctattaat ctcttgctgc aattctatag tttcttgaaa ttcttccgag
    12961 ttttctctca aagtatcgta atatttagta ttggctttcg tttgggattt ctttgataac
    13021 aagattaaga caaaactaaa taacgttggc aaaataacag ctaatcccat tttgacatta
    13081 ccaaccaaaa gcatcactga aataaaaggg aaaaacagag ccataccgcc ggctttaggt
    13141 attgcatgac tcatcgcatg ctcaatacct tcaacgtcag acatgatagt ttgagataaa
    13201 tctgataaat tatgttttga aaaataggat aatggtaaat ttgacaaatc gtctgcaatt
    13261 tgcactctta aatgcgcact ttctttataa gtactattat ataagttttc atactcccta
    13321 tctaacaacc aatacatgaa aagcaaagta acaaacgata cggcaaaata aagccaacgg
    13381 ggctttgtat tttctaaaac taattcgtct atcagcatca ttataatgaa tgctggtacc
    13441 atattaatgc agtatacaaa aaaactagaa atagaagctt tagttaaagc ttctgcgccc
    13501 tgatcactta aagcaaattt gtttttataa aaacttttca ttgtcaagcc tccaatcatt
    13561 tgcattctca taattagtga ccaattgttt atacacttga tgtttagcca ttaattcatt
    13621 atgatttcca cgttctacta ttttcccttc tgacaagaca agaatttcat ctacagattt
    13681 aatgctggat aatcgatgag ctatcataat aactgttttt tctttcatca gatgttgaaa
    13741 agccttttgt agttcatact cattatctgg atcaatagat gcactggctt catccataat
    13801 aacaatcttt gcatctttta aaattgctct ggcaatagct atacgttgtt tttctcctcc
    13861 tgacagataa actcctttac taccaattaa ggtatgctct ctttcgggaa atttttctaa
    13921 aatcgattcg caaccggcta atcttagcgc gtcaaatact ttacttgctg gcgcttgagg
    13981 atttgctata gaaacgttat cgtaaatact ctgattgaat agtttcgtat cttgaaagac
    14041 aaaagcaatt gtgttaataa ttgtgtcttt tgaatagtga tttaaaggct taccaccaat
    14101 tttaatctgt cccttatcca cattgtagaa acctgaaatt agtttagcta tcgtactctt
    14161 tccgcttcct gattttccta ctaatgcata aattcttttt tgttctaaaa ctaagttgaa
    14221 attttctaaa acacactctt ctttgtatga aaagctgaca ttttcaaatg caatgtcaca
    14281 atttttaatc tgtgtctcgt tcccatattt tagttggtct tgcttcattt ctaaataaag
    14341 ggagtctagt gtttcgactg catatcttgc ttcaaacaag tgcatactta catacattat
    14401 tctcatgaaa gaaacaaaaa taattccact taaaaaaaac aacatcaaca agtcaacagc
    14461 taatatcttg ggagtggtaa gagaagttat aaaaaaggtt ataggaacta tcaaaatgca
    14521 aactatgcca aaaaatagcc attgaaaaag aacaaaaggt gttttcccac tcaaagaata
    14581 cttatatgca aacttagcat aatcgttaat tgctttattc aaggctttaa atgatgttac
    14641 tttagcacca aatatcttta taacttgaat tccgcggata tattctaccg tttcagagct
    14701 taacacatct aacgattctt gatatttttg aataaaattt gtattctctg tcattttctt
    14761 taacaagaat acaccaatta ctaacatggc gattagaacc aatccaattt taaagttaat
    14821 gaaaaagcct aaggcaactg ataaaacagg tgttactatg gcttgagcac tatctggaat
    14881 caagtgagca attgccatat gtgttttagc cgcgttgtca tcaattgtct ttcgaatata
    14941 tcccgaagaa tttaaatcaa aaaaacgaaa acttgcttct gttaaaccgc taattccctt
    15001 ttttctcaaa ttagtctcta agcgaaaccc aaggatatga gaaaataagc cagaaaaaat
    15061 ataaaataaa gctcctaaag ttaaccaagc aacagtttga accgcataac tcttagctaa
    15121 atattcatct cctacaataa ttagagactg aagaaatttg taaatactgt aatatccata
    15181 gactgttaat acgacagaaa aatcagaaaa taaagtggct aaccatccgt agtatttcat
    15241 tttagggaca taactaaata attttttgta tactttcatt ttttcctccc ccgcattcaa
    15301 tttcttctct tattcgttta caactgttaa tgctttccgt aatagcgtaa attagagatg
    15361 tacaaacaga taaaaggtaa actacgttct attttttgta tttttttaag acttttcttc
    15421 caatcaatac accaatcata gataaacttg ctacaactaa cactgaaatg attccccata
    15481 tacctaccgc atagtggatc attgcatcca ttgttgcagc agattgtcct ttatctatcc
    15541 aatcttgacg aaaacgatct gtaaagaacc atataggaat ccactgtccg gcagcactgc
    15601 ctaattgcat tagagcccaa gacgcaactt gaggaattgc tcgatcatat ccaaatacag
    15661 atagaataat atcagctaag atcgccatac taatcgaact gataaaccaa ggtaagtacg
    15721 ccccgcccat taacgtaaaa aataacaata aaacaattgt gtaaatagta aaaattcctt
    15781 ttctagggta gttatagcta atataaacaa atatgattcc agataataat ccaaaaatcc
    15841 cgggggatat gctgtgacca aacgccccac caatactact aaccattatt gaaaaaaagt
    15901 aaattactag ggaaataact gtagctaaac caataaaaat ataatctttt gttttcatat
    15961 atattctcct caattctatt tcgttatgtc attaaaaatc tgttctatca aagttgaatc
    16021 ttctactcta agttttatat ctttttttat tccaccattt
//
LOCUS       DACCWK010000056        15770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCWK010000056 DACCWK010000000
VERSION     DACCWK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.056"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..358
                     /locus_tag="IVW65_001731"
     CDS             <1..358
                     /locus_tag="IVW65_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7900608.1"
                     /translation="IRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARAL
                     VSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILEL
                     SGKKLKKVNKMNLEVE"
     gene            358..1734
                     /locus_tag="IVW65_001732"
     CDS             358..1734
                     /locus_tag="IVW65_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7900609.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2020..3642)
                     /locus_tag="IVW65_001733"
     CDS             complement(2020..3642)
                     /locus_tag="IVW65_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP7900610.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            4294..5265
                     /locus_tag="IVW65_001734"
     CDS             4294..5265
                     /locus_tag="IVW65_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP7900611.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(5343..5579)
                     /locus_tag="IVW65_001735"
     CDS             complement(5343..5579)
                     /locus_tag="IVW65_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7900612.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(5548..5679)
                     /locus_tag="IVW65_001736"
     CDS             complement(5548..5679)
                     /locus_tag="IVW65_001736"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7900613.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(5755..6138)
                     /locus_tag="IVW65_001737"
     CDS             complement(5755..6138)
                     /locus_tag="IVW65_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7900614.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(6171..6956)
                     /locus_tag="IVW65_001738"
     CDS             complement(6171..6956)
                     /locus_tag="IVW65_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP7900615.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            7134..8234
                     /locus_tag="IVW65_001739"
     CDS             7134..8234
                     /locus_tag="IVW65_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7900616.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8224..9048
                     /locus_tag="IVW65_001740"
     CDS             8224..9048
                     /locus_tag="IVW65_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP7900617.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            9054..9860
                     /locus_tag="IVW65_001741"
     CDS             9054..9860
                     /locus_tag="IVW65_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7900618.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            9857..10687
                     /locus_tag="IVW65_001742"
     CDS             9857..10687
                     /locus_tag="IVW65_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7900619.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            10694..11725
                     /locus_tag="IVW65_001743"
     CDS             10694..11725
                     /locus_tag="IVW65_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900620.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(11832..13105)
                     /locus_tag="IVW65_001744"
                     /pseudo
     CDS             complement(11832..13105)
                     /locus_tag="IVW65_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            13332..13517
                     /locus_tag="IVW65_001745"
     CDS             13332..13517
                     /locus_tag="IVW65_001745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900621.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            13641..14357
                     /locus_tag="IVW65_001746"
     CDS             13641..14357
                     /locus_tag="IVW65_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7900622.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(14259..14779)
                     /locus_tag="IVW65_001747"
                     /pseudo
     CDS             complement(14259..14779)
                     /locus_tag="IVW65_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15067..15720
                     /locus_tag="IVW65_001748"
     CDS             15067..15720
                     /locus_tag="IVW65_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7900623.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         4778 a         2794 c         3420 g         4778 t
ORIGIN      
        1 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
       61 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
      121 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
      181 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
      241 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
      301 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
      361 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
      421 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
      481 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
      541 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
      601 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
      661 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
      721 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
      781 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
      841 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
      901 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
      961 gtagaaatta aaggtctatt cgatgggcat aatagcggtg tagtaagtgc agcacaagaa
     1021 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     1081 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     1141 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     1201 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     1261 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     1321 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     1381 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     1441 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     1501 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     1561 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     1621 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     1681 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     1741 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     1801 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     1861 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     1921 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     1981 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     2041 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     2101 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     2161 ttgattccta atagaaaacg agatgtctcc gacatcgcag actgtagaca aaagctgttc
     2221 caaactcgta actcgagctt ggaacagctt tttatctact tcgtttcgaa tttttgaaca
     2281 aaaaaataaa ggctccttgc ctttccatag ccaggtggct aatttcttta aattcattgc
     2341 agcaaacact agcatcgtat gcgtcgcgac tttttccaag cctcgatact tggtccaacg
     2401 catcccatgc ttttcttttg catccgcaaa tattcgttca atcgtttgtt tccgtttttt
     2461 gtaaattgat ttattcagat ctgttaggcg aagatgttcc acttcatcca tcagatctcc
     2521 ccaaacatgt ctcgtgatga ctcgttgatg atttttactt atggtacatt ggtttagcag
     2581 aggacagttc acacattctt ttggattgga tttgtattct cggtaccctt ctcgagtaac
     2641 cgttgaaaaa gcgagcattt gatcttgcgg acaaatataa caatcaaaat actcgtcata
     2701 gagaaactca ttttttgata atagctcctt tttccctttg ggccttgaat aaggcaaaca
     2761 aggtcgaagg ttcaaatgag tcaaaaagtg aacaaactta ggtgtcttat acccagcatc
     2821 agcgacaaca caattgattt caggaaaaag tctcttcgat tgtttgacga gctgaaaggc
     2881 gacttgactg tcatgaatat ttctaggagt gatgattgtg ctaagaataa atccattgtc
     2941 gtcgcatgaa gtatggacag aataagcaaa ttgtttttct cgttcgcctt tgacgtagta
     3001 gccgctttcc ggatcagctt tacttatttt tctttctttc agcttcactt ccttttctaa
     3061 gtccaaagta aaagggcgtt ttccagcctc tattcgttga cgattgatct catcttctaa
     3121 ttcatcctga taggctttcg cttcatcatg ggtcatttct ttcgtaaact tatgtttatt
     3181 ggcgttagct ttgatgtggg tagaatccag atacaaatta tcctctgtta caaaacctgc
     3241 tttaactgct tgttctaaaa tataggcaaa aatatcctca aacaccgtcg tttctctaaa
     3301 tcggcgaaca taattctttc caaaagttga gaaagggggg attttctcct cgaaggagta
     3361 gcctaaaaac cagcggtaag cgacattggt atcgacttcc ttaatggttt gtcgcatgga
     3421 acggataccg aataaatact gaataaacac caatttgatc agaatcactg gatcgatact
     3481 tgggcgacct aaatcggagt aagtggcttc gacgattgga taaataaatt caaaatcaag
     3541 cgcggcatca atctttcgaa cgagatgatc ttgaggaact agatcatcaa tcgtaagcat
     3601 cgcaaattga tttctaccat tcgtattgtt tttagacatc atagctatca ctccttgcat
     3661 tcgttttcat aaatctatta taaaagaaaa gactgtagac atcatccata aaatggactt
     3721 tgtctacagt ctggataacg tgattttttg ctgtaatgac aaggttttag ctgtttcaga
     3781 taccatgtat ccaaaaatag tttattgaga aaataggaga gtatttgata ctaactagta
     3841 ggaaaaaatg agataaaaag taaatggaat catttaaggt aatctgaaat aaaaaatata
     3901 cagccctgtg gtactttacc tacaaggctg tatatatttt ttactctcag cgtttttgac
     3961 tccgtgtctg actccgtttt attgattttg aatgttattc aatgaccatt taacaggtca
     4021 aatttgatgt ttttgattcc gtatgacgcc gtttgatatc tagtgatata gtgatttgag
     4081 tctacatcga ataagggaat aaaccaataa aaaatgattt agaatattaa aataacaaaa
     4141 aagaggacta cctgtattag tcctcttttt tgaccagatt ttgaccagtc tattttacat
     4201 cctagtatat ttatatgagc taattttatg attaaatggt aagtttttcc ttgtgtgttt
     4261 tagtttaaac tagtgtgtaa ttcaaaaatc tatatggaag gaacagtatt aagaatagaa
     4321 cagggttctc tacatgatgg tgctggttta cggaccgttg tttatttaaa gggctgccca
     4381 ttacgctgtg cctggtgttc gattcccgaa tcacaaagca agcagattga aaaaggattt
     4441 ggccagacca tgaccgctga agaggtaatg gatgagattg agaaggatgc tgttttttac
     4501 tttcactcgg atggcggagt gaccattagc ggaggcgagg cactggttca ggcggatttt
     4561 gctaaagaaa ttttgcaaaa atcgaaatac attggaatca ataccgtact ggaaaccagc
     4621 ttttgtggtg cttataatga aatccaaaag gtagctccct atgtggatac attgtttgtg
     4681 gatgtaaaaa tgtttaccag caagctccac aaacaatgga ccggcttaga taaccaacaa
     4741 attttgaaga atattcgtcg ctttttaatt gagtatccta actgcgaagt gcgaattcga
     4801 gttcctgtgg ttccaggtat caatatgaac ctgaccgagc tgctgacgat tgcttgtttt
     4861 gtggcagatc ttgatcggtt cgtaccgtta gaattactac cgtaccactg ctatggtatg
     4921 cacggttacc aagcattggg actggagtat ccgttggcag acacgccagc gccttcacct
     4981 aaagaaatgt ttgcattggc agatcaactg gctcgcacgg ttcctcattt gctggtcaca
     5041 gctttaacca aaacatttat ttatttattg aatccaagct ttatcttatg gataaatgaa
     5101 agacactcac tagctgcagc cagctcaaga atagagcttt tgctgaagtc agtgagtgtc
     5161 tttttacgat gttttttcga agttaatttg tcgaatgaac actggctcaa tcggtacatc
     5221 ttcactgttt gtcggctgag ctgcgatcgc tgctacgatt tctaaccctt ggtatacctg
     5281 tccaaaaaca gtataacgac catccagctc cggtagtcct ccaagctcca agtatgcttg
     5341 ctttacatta gcaggtcagc ctgcgccttt gtgttttgca caataaaaaa ctggttgccg
     5401 ttggtgtggg ggccgtggtc ccctaaggcc aaggcgccat taaaatgata aagtccgcga
     5461 tgcacttcat cagcgaaaaa gccctcatag ctggaaacag cttcatcacc agaaccatcc
     5521 aaagcgccac cttgaataac aaattcctta atgacacgag ccgaaaggtg tgttgtcata
     5581 aacgccttgc tcagctaggg agcgccagtt ggctacagcc aggggagctt ggtcaggaaa
     5641 taagcggaga cgaatttcac cagcatcggt gaccagcaca caaacttctt cctgctgcgg
     5701 tttttctaat tgaaagagag gaaggtttgc aactgaaacg ccttgatcct gccatcaaat
     5761 atcgaaaatc cgccactcga ccttcgcctg atcaccaagg cgagccattc ccaataaaac
     5821 ggtataggcg gtttcatccg cttgaaaggt caattgcagc tgtcctcgta aatcgttgta
     5881 gtcatctggg gcggctactt gataaaaaag atgaaactgc gcatgcaaat attccaaagg
     5941 ctgatcttta aaggctgcaa aataagcctg aactttttcc cgctgcaagc tgtcagccac
     6001 caaaaaattt tccagttgtt gttttggaac ggctgctaac aaggatcggc aaaccgcctc
     6061 aattttcaac gcatcccaat gtcgcgcaat taaataatgg atgggtgcct cttgattgta
     6121 gtcccacagg ttgctcattt ttttcaccac tttcttctat tatataggaa tcacaaaatc
     6181 ggcagcggcg tcaggcggcg ttcttttcct tgaatgacta gcaacttttc tacctgacag
     6241 tctgccaaca gctgactcat ttttttaaag cccagctgat aatcttcggc aacatgagca
     6301 tccgagccta aggtaaagag ctttccgccg acactttgat acaaaggaac ggcataacgg
     6361 taaaggtctt cgttgccgta tttcaagaag ctcttggcat ttaactccat cgccaattct
     6421 cgttcaacaa cctttttgaa aatcgttagc agctgagcct caaaatgctc cgcgaattct
     6481 tccgctgtga aggtgaagcg gcgtaatcca taatcaaagt gagtcaaaat atctccttct
     6541 ggaaaatccg tcaagacctg caacatctga tcaaaatatt ccttggtagt ttgaatggga
     6601 tctttggtta aaaccacatc gtccatataa tcaaagcggc cattttgatg aatacttacc
     6661 agctttaaat cataaggatg ctgagcgagg taatccacga tccgctcttc ttgtcctgga
     6721 acaacgccaa tctcgatacc tcgcagaaat ttggtggaat aggtttcttt taaagagtcc
     6781 agtttgttta tataggctgg ataatccgga atgtcatctt ttccggtaga tggattgttt
     6841 aaatcaaagt gatccgtaga aacgaaaaac tccggctgat aggctaaata gttttcaaat
     6901 ttttcctctg aatcaaagga aaaatacgtg tggagatgct gatcataata ctgcataggg
     6961 agcctccttt tttctactat cataccgcaa aagctttaga aggttaacgg gaattcgtcg
     7021 aaatttacaa accatataca acctttacat tgttcatgaa accttttcaa gaaactgatt
     7081 ctctcattac aaagctttgt tagaatggaa ttatcgaaag gggagaagaa tgaatgaaaa
     7141 tgaatgaaaa aattcgtaac atgactatcg gtttattttg tgcagcaaca gtaagtatcc
     7201 tgctgacagg ctgcggctca ggtgaagcaa cgaagcaatc ggctgcggca gacgactctt
     7261 tagatacgat tatagaaaaa gccaaggaag aaggagagat tgccagcttg ggaatgccgg
     7321 atacttgggc aaattgggta ggcacttgga acgaccttga aaaagaatac ggcttaagtc
     7381 acgtggatac ggatatgtcc agtgcagaag aattggctaa gtttgaatca gaaggaaaaa
     7441 atggaaccgc cgatattggt gatgtcggta tcagcttcgg tccgttggct gaaagcaaag
     7501 agttaacctt accttataag accaactatt gggaggaaat tccggattgg gcaaaggatg
     7561 atgatgggga ttggttgtta agctatacgg gaaccatttc ctttatcacc gataaagaga
     7621 atgtgaagca agcaccgact agctggaagg agctgcaaga gggtgactat caagttagcg
     7681 tgggggatgt gacgattgcc aaccaagcac aatttgctgt attggctgcg gcgattgcca
     7741 acggaggtga cgaaaaggat attcagccag gtctgaatta ctacaaaaaa atggcagaaa
     7801 aaggccgttt gtccaccgtt gatgccagcg tagcaaatct tgaaaaaggt gaaatcgatg
     7861 tggcagttgt ttgggacttc aatggactaa attatcgtga ccaaattgac tccgagcgtt
     7921 ttgaggtaac tgtgccaaca gacggctcca tcattagcgg gtatacaacc ttaatcaata
     7981 aaaatgcccc tcatcccaat gcggcaaagc tagcacggga atatatttta tctgatgaag
     8041 ggcaaatcaa tttggcaaag ggctatgcgc gacctattcg caaggtagag ctgcctaaag
     8101 aagtagctga aaaattactg ccggaggatg cttatgaagc ggcggtgcca gttgaagaca
     8161 atgaagcttg gaacaagacc tctgtagagc ttccagagct ttggcaggcg gaggtactga
     8221 cctatgcaaa gtagacttga caaactgatt gtagttatac tagacggctg tcgctacgac
     8281 acagccgtcg cccagcttgg gttcatgaat catttagttg agcaccagca agcaagctta
     8341 ttgaaggtaa aaagtgagct gcccagcaat tcacgtccgt tatacgaagt actgatgacc
     8401 ggcgttccag cctatgaaaa tggcatctat agtaattacc atgctcagcg atccaaagag
     8461 tggtcgctgt ttgaattagt taaggaagct ggcggaaaaa caggagccgc cgcttaccac
     8521 tggtacagcg agttgtacaa tcaagccccc tataatcgtt tggttgatcg catccagcat
     8581 caggaggagc gactgattca gcatggcatc ttttatagtg aggacactta ccctgattcg
     8641 catttatttg cggatgccca ttatttagtg aatcagtatc agccagactt tttggtcgta
     8701 cattcgatga atattgatga tgtcggtcac aaatttaccg ccgattctca tgaatatgca
     8761 gcagcggtta atcaggcgga cagattattg gctatttacc ttcctgaatg gctgcaaaaa
     8821 ggttatcaag tcgtggtgac tgcggatcac gggatggatg cgcacggact gcatggcggc
     8881 agcttggctg cacatcgtga agttcctttt tatttgattt caaataaacg ccaaaagctg
     8941 aaggaacaaa ctgtaccaca gctgctggcg gctccattgt tttgctggtt attaggaatt
     9001 gcaccgtcag agaaaatgca ggacattcaa gaattgatga ggggatgaaa gagatgaaaa
     9061 aacgcttggt tctggttcct gcctccttta tcttctgtgc atttttactg ctgccgctgc
     9121 tttttatggt tctgtgcagc ttgcacagtg atttaactaa ccagtggaca ttggccaatt
     9181 acagtgatat tttcagcaac aaatattaca cacaggcctt tgttaacagc gtactgattg
     9241 ctgtcagctc cagtgtgatt gggttaatcg ggacgtttat tttatgttta tgcttaatga
     9301 atctatcgac taaattgcag gagaaaatca cctttgtttc caatttggca gctaattttg
     9361 ctgggattcc gctggcattc tcttttatta ttttgttggg gaatgtaggt gtgctgaaaa
     9421 tgattttgcc atttctagcg gacttcaatt tgtattcttg gtggggattg accatcacgt
     9481 atacctattt ccaaattccg ttaggggtct tgtttttgta tcccagcatg aaggaagtga
     9541 aaaaggaatg gctggagtcc gcacaattgc tgggcgcttc atcagcgtat gcttggttca
     9601 aggtggcttt gccttttttg cgaccatcga ttgccagcac ctttgtcatc ttatttgcta
     9661 atggcatggg gacctatgaa acagcgtatg ccctgaccag cagtaatgta aatcttttaa
     9721 cgattcgaat tgcggcttta gtgtcgggag atgtgttcgc caagcctaat ttaggcagtg
     9781 cgttggcagt cgctttaggt gtcctgctgg taacggcaat gttgattatt caaaagaaag
     9841 gcaaggtggt gcagacgtga aaaagttaat aaaaccgatt ttgttctgct ttgtcctgta
     9901 ttacttgctg ccgattattg gaacgatttt gtatgccagc tccactaaat ggtcaaaatc
     9961 gcttttacct agtgatttta ccttgcaatg gttccaacag ctgctgacgg acagggaatt
    10021 tattgctgcg gtgggtcgct cacttttatt agcaggtgtg gtcttggtga cgattctgtt
    10081 gttgatgatt cccaccatta tttggattca tttgtatttc cctcgtttga atcgttggct
    10141 ggaaaagctg ctcctgctgc cgtatgcttt accaggagtg attttggtaa cggctttgct
    10201 tcgaacgtat gctgaaactg gtatcccaat gtttgtagtg ttggtaggtg cattgtttat
    10261 tacggcgttg ccgattgtct atcttagtct taacaaccag atgcgcttaa ttaatctgaa
    10321 ggaattagtg gatgcagcag aaaccttggg cgccccgatg tctacgatta ttatccaggt
    10381 gctgtttcca aacatccgta tcggtgtgac cctagtttct ctgatgattt tttcttcggt
    10441 ttttggggag tacatgctga cgaacctgtt gattggcggt cgatttcaga cggtacgcat
    10501 ctatatgctg cggcggatga acgaaaacgg ccatttggct agcgctgtga tggttttgta
    10561 tttgctgttt atggtgggaa ttgctttaac tacctttatg ctgacttatc gacaaaagca
    10621 acagctgtcc cttaagcaga cgagggaaga gaagcagtcg aaaaagaagc ccttaaagga
    10681 gttgtgacgg aaaatgtttt tagaaataca tgatctcaat ctgttttttg atgaaaaaca
    10741 gattttagct attgatgaaa ttcagattga aaagggtgag ctggttactt tactaggtcc
    10801 cagcgggtgc ggaaagtcta cgttgctgcg ctgtatcacg ggattggaac aaccaaagag
    10861 cggtgagatt gtgctggaca atgagaatat tgccgataag cctacgaagg atcgcaatat
    10921 cggctttgtt tttcaacagt atgcattgtt tccaactatg actgtttttg aaaatgtagc
    10981 ctttggtttg aaggtaaaga agctgagtcc tgaggtgatt caggaaaagg tttttgagat
    11041 gctatcattg gtggatatga cggagcaggc agataaaaat gtccagtttc tttctggcgg
    11101 tcaacgacaa cgggtggcac tggctcgctc attggtcact gaaccgaaag tcctgctgct
    11161 ggatgagcca ttaagtgctt tggatgcccg aattagaaaa cagctgcagc gggatttgcg
    11221 cgctatccag caatcgctag ggatgacgat gatttttgtg actcatgacc aagaagaggc
    11281 gatgcgaatc agtgatcgga tttttgtgat ggaagctggg cgggtggctc aggtttccac
    11341 ctcgaaggaa ttgtatcaga atcctcagag ccgatttgtt gcggaattta tcggcaacta
    11401 taatcgtttc gaatggtatg agctgaatca gtatacccac gagttttccg ctaagggcaa
    11461 tcgctgcatt tattatttaa gaccggagct gattcaattt gaaccggttc ctcagggggt
    11521 taagattccg gttctatgga aggaatcatt tattttaggc aacattcaac ggtatgtttt
    11581 tcaaacgctg gagggcaaag aaattttagt ggatcggttg aacgatcggg aagagaaaat
    11641 tagcgatgcc ttatacattc agctgaaaga tattctagca atccctgtgg aaattgatac
    11701 tcagcatttt gcacaacaaa gctagtgtat agtcggcaaa catttctggc tctttgtcaa
    11761 ataggactga tagagtgata taagaacatc taaggtagaa ttgattttct gctttagatg
    11821 tttttttatg tttaataagt gatcagatga ttgattaaga aaattctggt tctcatccta
    11881 acccgtaagc tattcgtttc aatgctttga tgtgggtatg cagattttct attttgccat
    11941 ttgagtaagg caactctaag gctaaacgaa tgccggattc atagtttata aacgcttttt
    12001 ttatatgatg cagttctctt ggaaccgact tgggcattgt ttttacaagc tgaaagaaga
    12061 tgtccgcttc tttgtcataa aaagcttgaa gaatttcttg atagtaagca taagctattc
    12121 gtagttcatc agaagtagaa agtagacgtt ctacgacttc actttcggtt aaataggccc
    12181 aaccaaaatt acgccatttt gtccatttat cataaacaag cgtgtcctgt cgtttgatca
    12241 gaagtcgaga taaagccttc acttgtcgat agtgtttggc ttgtattgga tcgtttcggt
    12301 cgaagctttt catgactcat agtcaaatat ccttaaaact gcgaatgagg tgcttgacga
    12361 tgtgaaagcg atccacaacg agtttggcat gaggaaagca ctcttttatc agcaaagcat
    12421 aagaggcgtt catatctgtt acgagtattt ttacgtttaa acgtgcacgt cgttcaaatt
    12481 gcataaaata agaaatgaga tctttcttct tacgtgaagg aagaatatcc agtaatttcc
    12541 cagtatcgcc atccatacaa gaaaaagaat agggaccgac ttgatttttc aatgaacgaa
    12601 attcatctac caatgaacag cgaggcaacc aatttatttt cacgtttgtc atggggagca
    12661 gagaagcgag tgttcgcgaa acggttgacc aagaaacgcc gcaatgttta gaaatcgtcg
    12721 tcattgcttg tttttcagtc aattcctcca atattttata atgtacttgt ttggcgataa
    12781 aacagttttt ttcaacaatg taggtattcg ccgtaaaata ggtgtgacag ccacgacaga
    12841 ggtaccgttg ctttgccaaa gcaagataag tccgttgatt cgcacactta ttgagaagga
    12901 ccagcgattt tttctttccg ttcttaacga tcagcccatg ttgattcacc tgtttacact
    12961 gaggacaggg ctgaatcgtt ttaatgagcg tggcagaaac aacgatactg ttgattgaat
    13021 gtattttttt cttctcgaca tgagtgattt gaatgtttcc atctttgatt cctagtaata
    13081 gtttgataag atgaagatca ggcatacaga acgctccttt gattgtgttg tggtgattca
    13141 attctaacgg agcttctgta tgttttgttt ttttatgctc aaaaggactg atagaatgat
    13201 ttactatcag tcctagaaat tatagcgcca catttctttc acctttgact tttcagacgg
    13261 cagtttttat gctgatactg tagagagagc aggacacagc ttttgcttgt tttgggtaag
    13321 ggggaaagaa gatgaccaaa gcacttattt ttgattttga tgggacgatg attgacagtg
    13381 aatggatgtt ttacgagagt attcatcact atttactgaa gcctatttac tggagcagtt
    13441 cggctataaa attccttttg cagactatca acaaggtgtt gggacggcag cagaactgtc
    13501 cttttatgaa gggataagtc aagcggttgg gagaccagtg gatgctgaac attttgagca
    13561 gcggatcagc agcactggaa aatcggagta gtctcaattc tgaactgcgg gagctagact
    13621 atttttttga agcacatccg atgattaagg aaaaaattga ttcctttgtg acgatcgatg
    13681 atcttcaggt cggcaaacca gatccagaag gttatttgtt gtgcttgaaa cggctgaggg
    13741 ttgaacaggc tgttgtagtt gaggatagtc cagtcggtgc ccaagcagca ccgcatatga
    13801 cagttgatgt acaagcgttg gatgcagatt ttttcgcctt tagcgggcat aaaatgtgcg
    13861 ggccgacagg aatcggtgta ttatatggca aacgtcaatt gttagaacaa atggagccag
    13921 tcgaattcgg cggtgaaatg atagattttg tcaatcttta cgatagttca tggaaagagc
    13981 tgccatggaa atttgaagct ggtactccga acattgcagg agcaatcgat tacttgaatg
    14041 agattggtat ggagaatatt catcgttatg agcaagaact ggttgcctac gttttaccta
    14101 aattacaagc gatcggcggt atcaccattt acggaccgca agatcctgaa catcatactg
    14161 gcgtggttgc ttttaatttg gatggcatcc atccccgtga tgtggcgact gctctggata
    14221 tggaaggtgt cgctgtacgt tcaggacacc attgtgcaca acggttcatc gacgatttct
    14281 gtttgactaa ttggtcgttt aaacagtcga tgataatgcc gtttgagcgg ctccaatgtc
    14341 gtggaatctt tgagtaagag tttccaatat cgcttgagcc gacggtactt cttctgattt
    14401 tcagattacg tgattgattt tcgtacagtt ctatgagaat cgcctgcttc agttcctttg
    14461 ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtctgc ccacaatcat
    14521 agcatagaaa acgggtacgt tttaattcta aaatagtcaa cttttctttg actttcaaca
    14581 tctgagtacg tgtcgtatag gaaccgttgc ggataatacg aatcgatcca cagtgttcac
    14641 aatgggaagg tgagtcatcc aatctccctt taataaactg cgcttgaacg cctttatatt
    14701 ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt
    14761 ttctagtatg attatccata gagcgactcc tttggtaggg tgatgttttg gtcgactaaa
    14821 ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc
    14881 gccttcgatt ttccaacaac acaaaatatt atagaagcct tttttctaaa ggatttcaag
    14941 gtgtctgttt gcatttatta aaaaaaagaa aaagtattgg caacagaagg cgtcaccaac
    15001 actagatatt atagagccat aatatgctgt ataatatcta gtgtataggc ttaagtggag
    15061 gtatatatga aaatattaac tgttgaagat gataatatga taagagaggg aataagtgaa
    15121 tatctttcag aatttggata tactgttatt caagctaaag atggaagaga agccttgtca
    15181 aaatttaata gcgatataaa tttggttatc ttagacattc agataccttt tataaatggt
    15241 ttagaagtgt tgaaagaaat tagaaagaaa agcaatttac caattctaat cttgactgca
    15301 tttagtgatg aagaatataa aattgatgca tttactaatt tagtagacgg atatgtagaa
    15361 aagccatttt cattgccggt cttaaaagct agaatagatt ctttaattaa gaagaattat
    15421 ggacatttag agaagtttga atataaggat ctatcggtta attttaatag ctatacagct
    15481 aaaataaatg gtgaagaaat agatgtaaat gcaaaagaac tagaagtatt aaaatgttta
    15541 ttggataata atggacaagt gttaacaaga atgcaaatta ttgattatgt atggaaagat
    15601 agcgaagaaa ctccttatga tcgagtaata gatgtatata taaaagaact tagaaaaaaa
    15661 ttacaattag actgtataac tactataaga aatgtaggat ataaattgga gagaaaatga
    15721 aaaaattaaa gatatttcca aaaatgttca tacaaatatt ttctgttctt
//
LOCUS       DACCWK010000057        15506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCWK010000057 DACCWK010000000
VERSION     DACCWK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.057"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(139..1248)
                     /gene="rpoD"
                     /locus_tag="IVW65_001749"
     CDS             complement(139..1248)
                     /gene="rpoD"
                     /locus_tag="IVW65_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7900624.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1272..3113)
                     /locus_tag="IVW65_001750"
     CDS             complement(1272..3113)
                     /locus_tag="IVW65_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7900625.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3295..5688)
                     /locus_tag="IVW65_001751"
     CDS             complement(3295..5688)
                     /locus_tag="IVW65_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7900626.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5795..6655)
                     /locus_tag="IVW65_001752"
     CDS             complement(5795..6655)
                     /locus_tag="IVW65_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7900627.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6714..7335)
                     /locus_tag="IVW65_001753"
                     /pseudo
     CDS             complement(6714..7335)
                     /locus_tag="IVW65_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
     gene            7544..8263
                     /locus_tag="IVW65_001754"
     CDS             7544..8263
                     /locus_tag="IVW65_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7900628.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8289..10880
                     /gene="mprF"
                     /locus_tag="IVW65_001755"
     CDS             8289..10880
                     /gene="mprF"
                     /locus_tag="IVW65_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7900629.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11068..12255)
                     /gene="tuf"
                     /locus_tag="IVW65_001756"
     CDS             complement(11068..12255)
                     /gene="tuf"
                     /locus_tag="IVW65_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7900630.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12623..12979
                     /locus_tag="IVW65_001757"
     CDS             12623..12979
                     /locus_tag="IVW65_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7900631.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13220..14224)
                     /locus_tag="IVW65_001758"
     CDS             complement(13220..14224)
                     /locus_tag="IVW65_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7900632.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14390..15241
                     /locus_tag="IVW65_001759"
     CDS             14390..15241
                     /locus_tag="IVW65_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7900633.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15258..15503
                     /locus_tag="IVW65_001760"
     CDS             15258..15503
                     /locus_tag="IVW65_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900634.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         4510 a         3217 c         2739 g         5040 t
ORIGIN      
        1 ttaggattta cttaaaaata atgaatgaat ttccttccta tagaaaaaaa agcttgagac
       61 tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa cagttttact gatttctctt
      121 ctggtcaata gtccacgctt attctaagaa atcttttaat tgttttgaac gagaaggatg
      181 gcgcaatttt cttaatgctt tagcctcgat ctgacgaata cgttcccgag taacaccaaa
      241 tactttccct acttcttcca atgttctcgt ccggccatca tctaaaccaa aacgtaagcg
      301 aaggacattc tcttcccgat cagtcaatgt atctagtaca tcttccaatt gttcttttag
      361 taactcataa gctgcgtgtt ctgctggact tgttgcttcc tggtcttcta tgaaatcacc
      421 taagtgagag tcatcttctt caccgattgg cgtttcaagg gaaacaggtt cttgcgcgat
      481 tttcaagatt tctcggactt tttctgttgg aagatccatt tccgcaccga tttcttctgg
      541 cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg atacgtatca atttattaat
      601 tgtttcaacc atatgaactg gaatacggat cgtacgtgct tggtcagcga ttgctcgagt
      661 gatggcttga cgaatccacc atgtagcata ggttgaaaac ttaaatcctt tgcggtagtc
      721 aaatttttca actgccttca tcagtcccat gtttccttct tggatcaagt caaggaactg
      781 catgcctcgt cctacataac gtttagcgat acttactact aaacgaaggt ttgcttctgc
      841 cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc aatgctaacg ctacttcttc
      901 ttccgcagtt aaaagggaga cacggccaat ttctttcaga tacatacgaa ccgggtcatt
      961 gatttttaca ccggttggcg cagaaagatc ttctgttttt gcttgttcag cttttttctc
     1021 agcactcttc aagctgtgaa ttgatggatc accattttca tccaccacac tgatacctgc
     1081 gtcttcaatc ttttggatca atttgtccat ttcatctgca ttcaaagtga atggtgtagc
     1141 aagctgattg gataaatcat catatacaac ttgtcgtttg ggtttgtttt ctttgatgaa
     1201 agctgctact gctgcttcat acgttttttt atctgttcca tttgccataa aagaaggccc
     1261 cccttgagta gctatttggc ttgttttaac tgtttcgtta aattgatgat ttcaatcgtc
     1321 aattcaagct caagctgttt gtttccagtt cgtctggctt cttgctgcat ttgttttttt
     1381 aatgttattt catcagctaa gccagatttg ctgatcacat ctagcaagtc ttggatttct
     1441 cgtggcgaac tttcttctgc caccggtaaa tattcgattt ccaccaccaa ccgccttaaa
     1501 ttttcatctt gcaaaaagtc taaaaactct gcaaggttga attcataatg ttgtgagaca
     1561 tatgcatcga ataaaaaata aacttctgca tatgcatcat gaataaactg aaattctcgt
     1621 tcttttaaaa tattacggac agaaacttct ttaaatgcac gatataatag aatctgctct
     1681 gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag tttcttgtct cgtaacctga
     1741 gtgtgttgac tttcctgagg ttcttgacga ttttgctgat ttgtccttcg aagatcgcgc
     1801 agttgttttt gtatcgtctc tctgccgatc tgaaactctg ttgacagttg agatagatac
     1861 atatcttgtt cgattggtga aacaactcgg ctaagttcaa ctagtaattc ttcgaggtat
     1921 tcgatcttgt ctttttcatt atctaaattt ttcccttgtt ttaaataacg tgatttaaaa
     1981 gtaaagactg tttcgcgccc atgagccact aattcttgga aagatgcttc tccatactta
     2041 cggagatatt catcaggatc aagcttttct ggaacgacta cgacagaaag tgctaatcga
     2101 ctgttttgcc gaagcaattc aatccctcga ttggttgctt caaaacctgc gttgtctccg
     2161 tcatagcaaa agaccaattc tttcgtcagc ttttccattt gttggatctg ttgagctgtt
     2221 agacttgtcc ccatagaagc taatccagtt ttgataccag cctgccatgc agcaatcaca
     2281 tccataaacc cttcgaacaa caaaatttct ccgtttttcc ggatatcgct tcttgcttta
     2341 tcaagattaa ataaaacttg tcttttattg aacagttctg tttctggact attcaagtat
     2401 ttgggctgat ctttttcctg ccctttttca ggtgcaagcc agcgtcctga aaaaccgatc
     2461 gtctggcctt gagaattacg aagtggaaac atcactcttt gataaaaacg atcagacata
     2521 cggccatcat catgcgtgac aaacaatcca gagcgttcta acaattcatt tgaaattgat
     2581 tcattcttga atacttgctg aagaaaagaa cgctcattag gtgcaaaacc tatgttgaat
     2641 tcttcaatta attctaaaga caacccacga ctttgcaaat attctaaagc tggctgtcca
     2701 gcttttgtat gcaatagcat gtgatgatag atttccgctg ctttttcatg agcagcaatc
     2761 agtttccccg tagcagattc aggattttgg acagctatag tacgctgacg ccattgatct
     2821 tctaacggta tctgttcgaa gtctgccact tttaccactg cttctggaaa acttagacct
     2881 tctagttctt gaagaaaagt aaacacgttt ccgcctttac cacaaccaaa gcaatgaaaa
     2941 atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt cttcatgaaa aggacacagc
     3001 cccaagtaat ttttaccaga ctttttcaac tggacatatt gtccaatcac ctcaacgata
     3061 ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa tccgttgagc cattttttca
     3121 ccccctgctc tattgatatc ttttgggttt aacagaagat atttcaggag aataaccggt
     3181 aaaaaatcgc actaactgaa atcagtgcga ttttaaagga cacaattata catgtttgat
     3241 tgtatcatat ttcattgctt tttcaagtat aacacatttc tatctcatta agcatcaatt
     3301 tgctactctt ttcagtaact gattttgaat atcagccttc cagtacaata ctcgaagaac
     3361 aatatttacg agcatgaata caaagaagta tcctacgctc tctcccaacc ctaagtttac
     3421 ttccaaaatt ccatggaaca gcattgcagc tacatatatc cctacgatcg tcgttactgc
     3481 taggaagatg cgcaatgggt tgaagggata acaagctttt acaactgcca tgcaactgat
     3541 cccaatcagc aagtaataca tcaaagtagt agtttcagca tttccccatg cgaatgtttt
     3601 tccaagtaga tacaccgcaa tgatattcac tactacaagc aaggcatttg gtaaagcatt
     3661 aataagtgct gtaggcaaat aacggtaagt gatttttctt ttatcttgtt caaaagataa
     3721 aaagaaggaa ggataccctt caatcgctaa atcaatcagc gtgatttgga tagggataaa
     3781 cggaaatcca attgctgtaa cggcacatat aatcgataaa ataaatgaat aaatcgtttt
     3841 gataaaaaag atgcctgaga ctttcgtcac attgttgacc actcggcgtc cttcaaacag
     3901 gacttctggc aatgtagtaa aatcagagtc caacaatacc agattcgcaa tctgacgtgt
     3961 tgctccatca ccttctgcca tagcaatgct gcaatctgct tctcttaacg ccaaaacatc
     4021 attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt tctttcaatt catttaccag
     4081 caatttcttt tgctgcggac tcactcgtcc aaaaaccgta tagcgatgag cagcttctcg
     4141 cacttccatt tcttcagtaa tttgagaaag atcgacatac gcatcataat tcggtaatcc
     4201 tgcacgtcta gcgatattgg agacagtaaa aggattgtct ccagaaatca ctttcagatc
     4261 cacaccttcc tcatgaagat aagctagtgt ttcttgagcg ttttttcgga ttggatcatc
     4321 gatttcaaac agagcaaccg gctcaacaaa tgctggtttt tcttcgttca attgctgatt
     4381 tggagcgatc cccaacataa gtacgcgata gccattcgct tgggctgttt ttagactgtc
     4441 cggaagccga tcttcttcca ccaaacgttc aggcgctcct acgtacacga ctcccagttc
     4501 tggaaattcc atcgcgcccc atttgcgatc agaagaaaat gcgactttgt ttgtcacatc
     4561 ataccgatta gaaagctcaa aataatctct catagcccgc atagtgatgt tgttatcact
     4621 gctttctgcc agatacgtcc ccataatttc tgggatggct ttctcgtaca gcggatgtag
     4681 tgcttctacc cgctggatac tcattttccc ttcagtaatg gtccctgttt tatccaagca
     4741 taatgtatcg acatgggcca gcgtttcaat tgaatacatg tcttgaacaa ggattcgttt
     4801 ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt attagcaata ccaaaccctt
     4861 tggaagcatg cctaacaaag ctgctgcaga tgcaataaca gaaattttga tagaagcatc
     4921 gcggatgaac agtgcttcca aaaatagtat gatccccaaa ggaataatca cgaaactagt
     4981 gaatttggat actttgcgaa tcgaatctac aagttcagaa cgaatagctt tgtgagtctt
     5041 tgcctcgcta gtcaagcgga cagcataatt atctgcacct actcggataa cttcagccat
     5101 gacagaaccg cttgtcaaaa agcttccaga aagcaattca gctccagttt ctttgacaat
     5161 caaatcagat tcgcctgtca aaagagcttc atttgcttct gcccgtccag atataacctg
     5221 taaatcggct ggtatctgtt ctcctgctcc gatttttacc aaatctccta ataccagctc
     5281 tttagtagaa atcgattgtt ctttaccgtc ccggatcact agtacttctg attgggaaac
     5341 aatcgataat ttccgtacaa gatttcttgc atgtatttct tgataaattc caataacgat
     5401 attgacgaca ataattgcta aatacgccat gttggaatac gcacctacaa ataacagaca
     5461 aatcccaata ccaaagttca gaaaattgaa taacgtcaat atattatcgc tgaatatttg
     5521 ttttgtactt tttgatgcat cttcctggta gtcattttgc tggccagaag ccattttctg
     5581 aactacctct tgctgtgtaa gtccattcat tttctttttc tccattttcc gccaactttc
     5641 aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt aaaaacaact tgtttttata
     5701 aaatttatag aaaaacacac gaatgatttt acaaaaaaga tgccaacctt tttgtttgaa
     5761 tcacccgtgt gctgttttta ttatcaatct atctttactt atattgttcc tccaaacggc
     5821 tcatccacca acctagtgct gtaccggcta taaaaagaac aggacaagct agtaccccag
     5881 caactcccaa tcccgcgtaa tcggtaggaa tgatcataat cgttgaagca aaaactacac
     5941 ctaaaatgaa atggaatagc ttgctatatg cctttgagaa aatgtaatcc atgatttttg
     6001 acaatccaag aacacaaaga atcccaccaa ttccgatcgg cacaatcact tcaagtcgta
     6061 cttctttaaa tccgtcagcc atcgctttat acaatcccat atacaccaaa aaattagaag
     6121 ggctcaatcc tgggacgatc atacccaaac caatcaatcc gccggcaatc agccatgttc
     6181 caaaattctg atcgacttgc ccgtcaaata attgagcgcc gtataataaa aacaaataag
     6241 ccagaacaaa agtaacaacc atgatgatca catcagaagt atctcttcct tttttccctg
     6301 cttctttcca taacgcagga acagttccag cgatacatcc gacaaaaaac cataatatcg
     6361 ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga aagtagaaag atgcctgtca
     6421 ctccgcctag accaacaggc agaaaaaaca aaacattttc tttgaaattt tttgtaaggt
     6481 gggctaaaaa agaaatcagc cgctcataca tgccaaagac agctgctaat gcaccaccgc
     6541 tgactcctgg caagataaat cctgaaccga taaacattcc cttcacaaac cgtaacatcc
     6601 agtctttggt tgtgtacccc acttcttgat tttccatttt ttgccttttt tccactattt
     6661 gcgcactcct tctttcattt aattttattc atgattgaac atttcctgct attttactta
     6721 aagacttttt cataggtctc tattattttt tcattcattt tgacaatatc aggataaaac
     6781 atgttacctc cattatgata gagatagccg ccattgtaaa gcaaggactg gatccgccaa
     6841 tatcggtatt gtttcccttc ctcatttcct aaaagtgggc tcagcacatc tttagaatac
     6901 gcttctgcta attctactgt gttttctccg ccatgatttt ttacataggc aatataatct
     6961 ttcccaaaat tatatgcttg gacagctgtc cataaatcgc atccttgttt ctttgcttct
     7021 actatcattt ccgccaaata tttgacacct tgatcaatac tgtcagctgg attatcaatg
     7081 atctcagctt ctccatgcag actttccgag atctgtccct tttccttttg attcggtcaa
     7141 tattatcgcc ttgatcaaat tctcgtagtt ttccatatga taattttttg cagctgttgt
     7201 tatttctatt tgatagcttt ccactcgttt taccgaacga taagttcttc ctcctaaaaa
     7261 caacaccgca ccaataaccg tcagaatgat catgtaaaaa agaatacgga taatagataa
     7321 tcgttttcgc ttcatttcat cacctagtta ttttgacatc tttttttatc ttactatttc
     7381 tttatcttct tggctatttc ttccttgatt tctgtgtacg ccttaagaaa aaatgaattt
     7441 tcctgtattt tttgacatct tacgaaaatg atcgttgttt ttcagtaaaa acaattgccc
     7501 aatgactgat ataatcgtta tactttacac agtaggtgat tatgtgaaaa aagaaaagaa
     7561 atatgcaaaa atgccagatg ggagcgaaat ttactatgaa aagagcggtc aaggcttccc
     7621 tctcttttta ctgcatggca atgacggcag cggccgcttt ttttcagaac aggtccctgt
     7681 actcgaacgc tactataccg tgtatctagt agatagccga ggacatggtc gttcgacaaa
     7741 cgaagcatct atgttgaatt ttcagttaat ggccgaggat ctgaatacga tcatgttgct
     7801 ggaaaaaatc gatcaggcag attttcttgg atttagtgat ggagcaaatc tagcactggt
     7861 atttgccagc agcttcccta aaaaagttca tcgattgatt ttaaattctg gaaacacgct
     7921 tgttaaaggc gttcgttttt ctgccagagt catcagtaat attcattatg catgggtatg
     7981 gttactctct ttatttcgcc caagtcttcg caaaaatctt ttagttatca aacttttgct
     8041 gcatgacatc gggcttacag aaaatgattt aaaaaaaatc aactccccca ctttgatcat
     8101 cgtgggaaaa aaagatgtaa tcaagctaaa acattctttg tatatagcta aaactatacc
     8161 aaaagcttcc tttgtgcttg tgaaagaaca aggacatgaa ttagctagaa aagatcctga
     8221 acgcttcaat cgagaagttc ttcaatttct ttctgaaaca taactattag gaatagaagg
     8281 tgatttattt gttaaaaaat accatacaat ggctgaaaaa ccacctagga ctgttcaaaa
     8341 caatcttttt gatttctgtc attgtcatta ttgtcggtga attgatgtcc atcggtaaaa
     8401 ctctatctat ccagcaatta ggagaaacgt ttgccactat acctgtatgg aaaagcggac
     8461 taatgctatt gattggtctt atctctgtgt tacctatggt tggttatgat attattttga
     8521 atcggatgct tgaacaaaag cagaatcctc gttacctgtt tgagacaagc tggctgatca
     8581 atacgatcaa caatattgcc ggtttcggcg ggtttgttag tgtggggctt cgctctgaac
     8641 tatatggaca aaagaaagag agcagaaacg tgatccaagc attatcgaaa gttttcttgt
     8701 ttttgatggc tggactttct atttacagtc tgctttcttt tttacttgta gtcttcacgc
     8761 cagttgctcc ttttttgaag caatattgga tatggttgat cggcggcggt ctatattttc
     8821 ctgtcgtctt tttatttact actttcaaaa agaaaggatt ccttggtggt acctcactta
     8881 aaactcaagg acagctattg ctagtttcat tgttggaatg gaccggtgtc ctgtttagtt
     8941 tcctttctgt tggttattta atggaaatcc ggattgatct gtggcaaaca attccattat
     9001 tcattgctgc ttcagtgata ggaatcgtgt ctatgattcc aggagaaatt gggagtttcg
     9061 atgtaatgat gattatcggg ttgtccgcta ttggtgtgcc aagagaaata gtcgttgtgt
     9121 ggatcttgct ctatcgcctg ttctactaca tcattccgtt tttgattgga atcgtctttt
     9181 tcttcaaaaa tatcggttcc acttttgatc agcgttattc tggtatccct aaacaattag
     9241 caacagaaat tgcgcataaa atcgtggttg tcttattata cttttctgga atcatgctag
     9301 tactttctgc cactatcccg caagctttta cagagtttcg ctggcttcat agtttgaacc
     9361 cattgaaatt tcattttatt atccaatttc ctagtatact attgggattt cttttaatca
     9421 ttatgggcag agggatcgca gcacgtgtga aacgagcata tttgcctaca atcttcttga
     9481 ttgttctagc tttactttat gttttattaa gtgattttag tttcacaccg gtggttttct
     9541 tgtctattct gcttctgatc attcttgctt ctaaaaacga attatttcgg gaacaattga
     9601 tctattcatg ggagtggcgt acgatcgatg gaatcattat tggtgcactg tccttgcttt
     9661 acatcgttat tggggtatac aatctgccag atttccctca tcggcgtcac cattttattt
     9721 cctttttctt atttccttct gaaaagattt ggttttcggg attgttggcc atcatcgctg
     9781 ttagtttcat gatcgttcta tttgttcatt tccttcaagg agaaaagaaa cagattggcg
     9841 aagcctttaa tgaagaaaag gcactgaaga tccttacaac ttatggagga aactccgata
     9901 gtcaattgat cttcttaaaa gataagcgga tgtttgccta tgaaaaagac ggtgaaccaa
     9961 ctgttcttct ccaattcgct tgcttcaata ataaatgtat tgtaatgggt gatccttctg
    10021 gtaagaaaga agattttcca gaagcaatcg aagcttttat cgaagaaaca gatcgactat
    10081 gctatttacc agttttctac gaaacaagcg aagagattgt tatgattttg catgaattcg
    10141 gctacgattt cattaagatg ggggaagaag cttatgtaga cctgaatagt tttacaactt
    10201 cagggaaaaa aatgaagggc actcgtgctg tcttgaatcg gatcgaacga gaaggattta
    10261 catttgatgt cttacaacca ccattttcta ctgagcaaat gagtactttc aaaaatatct
    10321 ctgataattg gttgggttca cgaaaggaaa aagggttttc tttaggcttt ttctctgaag
    10381 attatctaca aagagcgcct attgcagttg tcaaaaatac ccaagaagaa attgtcgctt
    10441 ttgcaacgat catgcctact tacacgaaca ataaagtagg aacgattgat ctgatgcgat
    10501 atgattctaa aaaagcacct tctggcagta tggatttctt gttcttgcat ttgtttgcct
    10561 atatgaaaga agaacatatc caatggttca atttagggat ggcccctctt tcaaatgtcg
    10621 gaacttccag aaagagtttt ttacaggaaa ggattgcata cttggtttac gagtttggtt
    10681 ctcattttta ttctttccat ggattaagag aatataaaag caagtacgct acaaactggg
    10741 tctcacgtta tactctatat tctagagata gttggattgc atatgttatg attgctttgc
    10801 tgattattga taatgcgcca gttgagcaga cacaaggtag aataagtggt ttgaaaaaat
    10861 ggatacgaag aaagtattaa aaactgcata ccgatagatg aacactctca tgatgaataa
    10921 aagagactga agtaaaacga aagttcatgc ttctacaatc tcttcttata caattgaaga
    10981 acaaagagcg gtccaaacag aaatcctgtt tggaccgctc ttttatttta aacatttttc
    11041 attaccaaaa tattcgagtc gcgtttctta tgcttcgatc tctgtaacga tacctgaacc
    11101 tactgtccgt ccgccttcac gaattgagaa tgtcgttccg ttttcaacag cgattggatg
    11161 gatcaagtca acatcaatcg tcacgttgtc accaggcatg accatttctg tatcttctgg
    11221 caatgtgatt gttcctgtaa catcagttgt gcggaagtag aactgtggac gatagttgtt
    11281 gaagaatgga gtatgacgtc caccttcttc ttttgtcaac acgtacactt ctgctttgaa
    11341 ttttgtatgt ggagtaatgg aacctggttt agcaagtact tgtccacgtt cgatatcgtc
    11401 acgttggatc ccccgtaaca acacgcctac gttatcccca gcttccccgt aatctaacgt
    11461 tttacggaac atttctacac ctgttacaac tgctttttgt gtttcaggtt tgatccctac
    11521 gatctctacc tcatcaccga cacgaacagc accacgatca atacgacctg aggcaacagt
    11581 tcctcgacct gtaattgaga agacgtcttc cactggcaat agtaatggtt tatctgtatc
    11641 acgttctggt gttgggatat attcatctac tgtatccatc aattccataa tagcagcttc
    11701 agcatctgga tcgccttgca atgcttttaa tgcggaacct ttgatcacag gagtgtcatc
    11761 gcctgggaaa ccatattcgc tcaataactc gcgaacttcc atttctacca aatcgatcaa
    11821 ttcttcatca tcgactaaat caactttgtt caagaaaaca atcaggtatt ttacaccaac
    11881 ttggcgtgac aataaaatgt gttcacgtgt ttgaggcatc ggaccgtcag ttgcagaaac
    11941 aactaagatt gcaccatcca tttgtgctgc ccctgtaatc atgtttttca catagtccgc
    12001 gtgtcctggt gcatcaatat gcgcgtagtg gcgtttttct gtttcatatt ctacgtgtgc
    12061 ggtattgatc gtaatcccac gttcacgttc ttctggagct gcatcaatac tagcatagtc
    12121 ttgaggattt gctaggcctt ttttgcctaa tactgtagtg atcgcagcag ttaaagtagt
    12181 tttcccatgg tcaacgtgtc caatcgtacc aatgttaacg tgtggtttac ttctgtcata
    12241 atgttctttt gccataattt acaaacctcc taaataatat agatcactga aaaatttcag
    12301 ctctcacctt tttattatag tctttttttg actaaatcaa aaagaatacg tccgacttac
    12361 tttttgtaag attcgaacta agcttattat acgtcaatat tggtcaaagt caactaagag
    12421 cactcgtctg tcttatacgc gaaaaaagta ccttttataa ataaaaaaat gtgttttttg
    12481 agaaaaactt caagatagtc cttaactttc ttttgaagca ataagaacaa aatgaggaaa
    12541 agtttaaaat tcagctgttg ccaacaactt tcaatagacg aaatcttcta tttcgttaat
    12601 aataaggaaa gaggtgaagt acatgaaaaa attaattggc gtacttgtcg ttttaggcat
    12661 atttctcggc ggaagttttg cagcttatca ttatttttac ggcggcgaag cttattatac
    12721 aaaaatcacg acgaatggcg aaatgtcagc tacccaagca gataacggag agaaattcac
    12781 gacttatacc tacaagcaag cggcttataa taaagatggt gaaaaaaaac aggtgacgct
    12841 tcgcgaagaa agagaacatc cattaaagat gaatgcgtat cttaaactca aagtcaatcc
    12901 tcgtaaaggt gtattaagct gggaagaaat aaaggcttct gaagtgccta aaaatgcggc
    12961 agaaaaaatc gataattagt aaatggtctt aaaattttat tagataaaaa gaggctggga
    13021 cagaagcgtt taactccaag aaataagaag gagttgcttc tgcttccgcc gtttatacgt
    13081 gtttagagcg tgagacaaaa gtgttcttta cttttgtctc acgctctttt tttgttatat
    13141 tcgtcttttc cgatccttct cttgacacaa ttcatttaac tgatcactgg tacttgaaca
    13201 cctaatattc tagtcatttt tataaacgat tcaatacttc atccacattt ttgatcatca
    13261 cggatatcgt accatccgga ttattcacaa attcaatcaa atctgggttg cgatatacat
    13321 ctactggtac gatcagttcg atcccgtttg atagcttgaa tttttgtttt ccaaattttt
    13381 tctcagaaat ttcttgtact tcttttacag gaggtgcttc tgggacaaat ccttgctctt
    13441 gtacttcttc tttaaaagcc atttttgccg tgatgttttc cttgaagact ttttccgcaa
    13501 tcatctcatg atccaaccga cctttttctt ctatcgtatc atagacagct tctttcaaat
    13561 cagccatgat atcaaattct tctgtctcaa atttttttcc aagatgcttg gctacttttt
    13621 ggatcacttt tacattttct tctaaagaag gtgcaggaac actttcgatc actcttccag
    13681 aaaaataaag ctcttttttg ccagaaaact catatttttt ttcaattaat tcgtaggtcc
    13741 aatcagtcaa attcacagcg aatgcttcat ctgctttttg tgttttcgcc cctagaattg
    13801 ctcgattcaa aatcaagcgg ttgtcgatcc ctgcttcgtc tgcttcaaca aagtgagtgt
    13861 aagcctcacg ataattgact ttcaataaag ataagtacat cactgtatct aattcataca
    13921 agacaacaaa tacatctgca ctaggtgctt cttcactttc ttggtagata tcgaaccacc
    13981 gctgaacgat tgcttcactt gcttccacaa aattttcttg agcgatttta agtgattcaa
    14041 cgatcatgct ttcttctgct agttgtccag tttttgtttg agcagaagag attttttgga
    14101 tcttcttttg aagaaaatca cgaatatatt cttttgtcag atccagctct ttttgtgaaa
    14161 agactggaga accagtttct ctgtcaataa tatgcagaat tgcttttttt aaatagatat
    14221 ccattttctc attcccttct gtcttagttt acggaaaaaa aatcaaaaat accagaatca
    14281 aaatcatttt cttttaaaca aaaatttcaa gagtagaaac aaaatggatt ctttttttcg
    14341 aaaaatgcgc tacacttata gttgaacgaa taaaaaagga gctgaaagta tgaattcatt
    14401 aacaagcact tatcgtctat caaatggtta cgaaatcccg gtagtaggat tcggcacatg
    14461 gcaaacacct gatggggatg tcgctgtttc ctctgttaaa gaagcgctgg ctgcaggtta
    14521 tcgccatatc gatactgccc aaggatacaa gaatgaagaa agcgtcggcc aagcgattaa
    14581 ggaaagtgga attcctagag aagagatatt cttgacaaca aaattatgga atgcaaacca
    14641 cagctatgaa cttgtgatgt cttcttttga agaatcattg aagaagctcc aaacagatta
    14701 tttagatctg tttttgatcc actggccaaa ccctgttgct tttcgtgata attgggaaca
    14761 agcaaatgca gatacatggc gagcatttga agaactgtat gaagcaggta aaatcaaagc
    14821 aatcggtgtg agcaatttct taccccacca tctagataca ctagcaaaaa cagcaaaaat
    14881 tatgccgatg gtcaatcaag ttttccttgc accaggcgaa ttacagacaa ctgtagttga
    14941 gtatgcaaaa aaacatgata tgatcctcga agcatatagt ccattaggca ctggtaaaat
    15001 cttcgatgtg cctgaaatga aacaaatcgc tgaagcacat gacaagacga ttgcacaagt
    15061 tgctttgcgt tggtcgctgc aacacgaatt ccttcctctt cctaagtcag tgacaccaag
    15121 cagaatcaaa gaaaatactg aattatttga ttttgaatta acagaagaag aaatgaaaca
    15181 aatcgatcag ttagatggtg tagtcggcaa agcaaaagac ccagatacta cccaatttta
    15241 agtcgaaagg tgattttatg gatccaatcg aacgcttgaa ttctctctcg gaagaagtta
    15301 cccagacttt tcattccgat tttgtctttt tgatcgatgc agagaaaatc caacattttc
    15361 ctgcaagaaa ctggacacat gatcaaatca tcgaagaact gaaaaaacgg ttcgatcatt
    15421 cgctaatggt caaaccctgg cacgaacatg aagtcattta ttcacctgaa ctacctgttt
    15481 ttgccttgat accgaaaaaa tagaaa
//
LOCUS       DACCWK010000058        14724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCWK010000058 DACCWK010000000
VERSION     DACCWK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.058"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(137..1717)
                     /locus_tag="IVW65_001761"
     CDS             complement(137..1717)
                     /locus_tag="IVW65_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7900635.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2118..3494)
                     /locus_tag="IVW65_001762"
     CDS             complement(2118..3494)
                     /locus_tag="IVW65_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7900636.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3622..4740)
                     /locus_tag="IVW65_001763"
     CDS             complement(3622..4740)
                     /locus_tag="IVW65_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7900637.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4882..5316
                     /locus_tag="IVW65_001764"
     CDS             4882..5316
                     /locus_tag="IVW65_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7900638.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5394..5927)
                     /locus_tag="IVW65_001765"
     CDS             complement(5394..5927)
                     /locus_tag="IVW65_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7900639.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="IVW65_001766"
     CDS             complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="IVW65_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7900640.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7192..7989)
                     /gene="recX"
                     /locus_tag="IVW65_001767"
     CDS             complement(7192..7989)
                     /gene="recX"
                     /locus_tag="IVW65_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7900641.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8096..9478
                     /gene="rlmD"
                     /locus_tag="IVW65_001768"
     CDS             8096..9478
                     /gene="rlmD"
                     /locus_tag="IVW65_001768"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7900642.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9652..10230)
                     /locus_tag="IVW65_001769"
     CDS             complement(9652..10230)
                     /locus_tag="IVW65_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7900643.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10552..11022
                     /locus_tag="IVW65_001770"
     CDS             10552..11022
                     /locus_tag="IVW65_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7900644.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11130..12356)
                     /locus_tag="IVW65_001771"
     CDS             complement(11130..12356)
                     /locus_tag="IVW65_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7900645.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13110..13310)
                     /locus_tag="IVW65_001772"
     CDS             complement(13110..13310)
                     /locus_tag="IVW65_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7900646.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13444..14052)
                     /locus_tag="IVW65_001773"
     CDS             complement(13444..14052)
                     /locus_tag="IVW65_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7900647.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14137..14607)
                     /locus_tag="IVW65_001774"
     CDS             complement(14137..14607)
                     /locus_tag="IVW65_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7900648.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         4398 a         2974 c         2436 g         4916 t
ORIGIN      
        1 agacatttct atgattttaa tatagtaaaa agagccgtca cggaaaatcc gtcacggctc
       61 taaacaaaat atgagaggct gtttttgttt ggctattctt attacttgat gcagggcagc
      121 ttgtgtcaag acttctttac atcaaacttt ttaattttac attgggatat ttatccgcaa
      181 accagcgttc tgcaaattgg ttctcgaata agaacaacgg ttggtcgaaa cggtcacgag
      241 ccaagatatt gcgacttgaa ctcatcttct catccaaatc ttctggatcg atccagcgag
      301 cgattttgtg tcccattgga gacatgatta cttcggcgtt atactcattt aacatacgat
      361 attggaatac ttcgaattgt agctgaccaa cagctccaat gatatattct tccgttagat
      421 atgttttata aagctgaatc gctccttctt gaactaattg atagattcct ttgtggaagg
      481 atttttgttt catgacattt tttgcagtca ctttcataaa cagctctgga gtaaatgaag
      541 ggagttcttc ataagcaact tttagttttc cttcgaacaa tgtatcccca atctgataat
      601 tgcctgtatc atatacaccg atgatatcgc ctgctactgc tttttcgacg ttttctctcg
      661 aatctgccat aaattgtgtg acattgctta atttcagttt tttatttgta cgttccaacc
      721 agacatccat tcctcgttcg aatgttccag aacagattcg gacaaaagcg attcggtcac
      781 gatgggctgg attcatattt gcttggatct tgaatacgaa acctgaaaat tcttcctcat
      841 aaggacttac catctgatca tcttgtgttt tgtgtccata aggagctggt gccaattcaa
      901 cgaaagtctc taaaaaagtc tgtacaccga agttagtcaa tgcagaacca aaaaatactg
      961 gtgtctgatc gccacgagca attttttctt gattaaatgc atctccagcc tctttgatta
     1021 gttctacttc ttctaagact tgttcatata aggaattttc agttagagga tgattttcgg
     1081 gaagcagtcc atcttcatcc aattctacta atcgttcatc ctgataattt tccggacgat
     1141 aaaactctac acgattatgg tagatatcat aaagaccttc tagcccttta cccataccga
     1201 ttggccaatt catcggataa gattcgatat ctaatagctc ttctaattct tctaacaatt
     1261 ccaatggttc acgaccatca cgatctagtt tgttgataaa agtaaatatc gggatgccac
     1321 gtttttttac aacttggaat aatttctttg tctgtgcttc gatccctttt gcactatcga
     1381 ttaccatgac cgcactatcc acggccatca aagtacgata agtatcttca gaaaaatctt
     1441 catgtcctgg tgtatccaag atatttactc gtttcccttg ataatcaaac tgcataacag
     1501 aactagtaac agaaatccca cgttgtttct cgatttccat ccagtctgat ttggcaaaat
     1561 tacctgtttt tttgcctttt acggtcccgg cttgacggat agccccacca aacaatagta
     1621 attgttctgt aatcgttgtt ttcccagcat ccggatggga aatgatcgca aaagttctgc
     1681 gattgtccac ttgttgcttt aattttggat tattcataac ctaactccat ttcttccatt
     1741 caattattta tattttctct aggcacagat tgtccgatct cttaaagaaa gacaatttca
     1801 tatagggctg tgacaaaagc cgtgtagcag tctctttttc cgagtaaacg gcgttctaga
     1861 gacagcctat cagcaataag cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg
     1921 ctgctcttat tacttaatgc aggatagctt tttcacaacc tttgtcaact actacaactg
     1981 aatcagtata gcatggattc agcgaatgaa aaagccacca aaccataaga gtcaaatatt
     2041 tttttgtcag ctgactaaaa gaaaagacgt gttgcttgtt tattcaagca acacgtcttt
     2101 cataaccaag tttccaatta tcttcttggc tcgtcatctt cgaattcttt tctgaagtat
     2161 cttcgttctt cttcaggttc cgcttctgtt tcttccggat catgaaaaat gacacggatt
     2221 ttaaggattc gagaaccttc catttcttca gaaaccagcg taatattatc gacatcaaac
     2281 gataactttt cgccttcatc cggaatcatg cctaatgctg taatcaaata accagccatc
     2341 gtatctacat cactcatatg cagatcactg tcaaaagctt cattgaactc atcaatcagc
     2401 atacgtcctt ggatgatata ttcgtgttca tcgatttttt cataaagatt ctcaacttca
     2461 tctgattcat catcgatttc tccgacgatt tcttctaaaa ggtcttctag tgttgctaac
     2521 ccaacgacgc cgccatattc atctaataaa atagccatct gattctgcgt ctttttcatt
     2581 tcatataaca gatcatcaat aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt
     2641 ttcttgatat ctaaattatc aaagccgaat ttatgggcag cttttagtaa attctttgta
     2701 tgaaggattc cgactacttt atctttgtct tcgttataaa cagggattct tgaatagttt
     2761 tcggataaaa cttcattcac attttcttcc acagtgtcgt tgatatcaat cataaatgca
     2821 tccgttcgtg ggaccatgac ttctcgggca actttcgtat caagagaaaa aaccccttgg
     2881 agcatttcta attcttcatt attcaatacg ccttcgtttt caagcatata acgcatttca
     2941 tctctggtca tttttgaatc ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa
     3001 cttgtggaag cagaaagcag ccagacgaag ggcttagcta ctacaccaat caagcgtatc
     3061 agccctgaag tgaactttgc gacttcttct gatttattta aagcaatacg tttagggtac
     3121 aattctccaa aaacaatcga tacataggtc aaaatagcca gcacaatgat atttgcaata
     3181 ttttttgccg ctgtgccgcc acctaatacc ggagctaacc gttccgataa agtattagct
     3241 agtgaagcac cagacaaaat gttgaccaaa gtgattccta cttggattgt cgataggaaa
     3301 ttatttggat tctgcaatat ttttaaaagc ttttgagatt tcacatctcc ttcatcagct
     3361 ttttgctcga ttcgattctt attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa
     3421 gcattgatca atgtcaaaac gatcaataat aaaatctgcg ctaacagcga ctgactctca
     3481 gggtcagcat tcataatcgc attctctcct tataaaatta atttttagtt tacgatagaa
     3541 aaaaacgtgg cactatagcc acgttaaaat ggtatcacaa agcaattttc tttgcaataa
     3601 aaacaagtgt actaaaacta cctacgaggt cgtagcaatt ttcttcttgt tttcattttg
     3661 cttgtcttct ttgacgattt tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa
     3721 aggaacacct aagaagatac caagcaatcc ggcaatattt ccagctacta gaagaacgat
     3781 aatgatcgtt aatggatgga ttttcagtga tttcccaatt acattaggat aaatgatatt
     3841 cccatccaac tgttggacga ttagcacaac cacacaacaa agtaatgctt ttaccggatc
     3901 attgaagact gttactagaa atgcaggtgc tagtcctaga taaggtccta aatatggaat
     3961 taggtttgtc aatcccgcaa tgaccccaaa taagaaagca taatccacac caattaccat
     4021 atatcctaaa aatgtgaagg taccaacgaa taaacattcg attgcttgtc cgctaatgta
     4081 attagccaat gttttattca gttgccccaa taactccaca atttggtttc ttcttttttc
     4141 tgggaaaaac tgttggatgt ttggcactag tttttctccg tctttcaaca tatagaacaa
     4201 gatgaatggt gctgtaatga ttactacggt tgccgaagcg atcgttccaa cgatcgaacc
     4261 taaactattg gatactccac taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa
     4321 ttgctcaaaa tatttattga tatctacttc cttgaacaaa ggcaattcgg ctacctcttt
     4381 taaccagctt tctacctgtt tgatgaaatc aggcatatta gatgccaatg aggaaatttg
     4441 ctgtactaga cttgggatga cactaagtaa aatccaaata attgctgcaa ctaaaaggag
     4501 cagcacaatt gcgacagccc atattctttt gatttttgct ttcatcaaca gcttgacaat
     4561 aggattcaat aaatagtaaa gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa
     4621 aaatgtcccg atcggttcga aaataaagtt tatttctgat gaaatcatga tcaatgcagc
     4681 tatgatcaat aattcaagcg accaaaacat tagtttagac tgtctaagtt tttcaaacaa
     4741 tagatttcct cctttctttc ttactagtat atattatatt cgttaaaaat tcttttattc
     4801 tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata
     4861 cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt
     4921 tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg
     4981 tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa
     5041 aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag
     5101 cggatggcga ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa
     5161 gcagaaaact ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca
     5221 gctgaaactt tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct
     5281 ggcatgcctc atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa
     5341 aacagcaagc ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta
     5401 cgcgacaatt gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca
     5461 tctccattat tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata
     5521 tcgttcgggt aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt
     5581 ttctcgccgt caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca
     5641 tcgtccagca aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc
     5701 attgctatta cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt
     5761 acccatcggc gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag
     5821 tgttcgcttg tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc
     5881 ttgtaacttt ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct
     5941 taagcagcat tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata
     6001 tcaataaaaa tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga
     6061 tctaccagtt tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac
     6121 cacttcgaag ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa
     6181 aaaaggagaa catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt
     6241 gtctgccaaa caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact
     6301 aggtcatcaa acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac
     6361 tcctcttgtg tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta
     6421 tctctttctt caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa
     6481 acgtcttttg gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg
     6541 ttagctaaac aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag
     6601 atagctgaac ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg
     6661 attttccgca tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca
     6721 atgcaaaaca atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt
     6781 ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta
     6841 atttcttcag gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca
     6901 gcttggatgt tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt
     6961 tcttctggag catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa
     7021 taatcgatca ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga
     7081 taaggatcac gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg
     7141 aactgatctt gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact
     7201 ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata
     7261 taagctttgc tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca
     7321 tagtttatct ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg
     7381 aagatcagct atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg
     7441 ttggattttt ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc
     7501 ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc
     7561 aatgatttca ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt
     7621 gtcacttaag cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag
     7681 gtccaattct tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc
     7741 ttttaagtat gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc
     7801 taggtgaaaa ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc
     7861 taattcccgt ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc
     7921 ttctccttca gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac
     7981 ggttaacata aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt
     8041 aatcaggtat tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc
     8101 agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa
     8161 tggtgaagga atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa
     8221 agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat
     8281 aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg
     8341 cggtggctgt caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct
     8401 gcttttgcaa gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc
     8461 gacgatcggg atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa
     8521 aaataagcag actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt
     8581 ccctataaca gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt
     8641 tcaactattg aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt
     8701 ccgcacaatc atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac
     8761 tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc
     8821 cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga
     8881 tgaaacgatc cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt
     8941 cgatctttct cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg
     9001 cgaaggaatc aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg
     9061 agttgggacg atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac
     9121 cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac
     9181 tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca
     9241 gcctgatggt atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc
     9301 attattgaaa taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc
     9361 cagagatctg gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat
     9421 gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat
     9481 gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata
     9541 gctcttcatg ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt
     9601 tatttaaaaa aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat
     9661 attcacgtct atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa
     9721 aatcacatta tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg
     9781 tggaacagtt gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg
     9841 ttgattagga ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg
     9901 tgctgttaaa ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct
     9961 gttagatgtc tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc
    10021 atcaatgaat gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac
    10081 attttgattt aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt
    10141 aatataacca tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc
    10201 aataaacaat aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat
    10261 tgtaaacgct ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa
    10321 aaaaggtgaa aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag
    10381 aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt
    10441 tcaacttttt aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt
    10501 cttgctgtga gccttcatgg taaactacta ttatagaaag gagttagtaa aatgaagatg
    10561 ataaaagata ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca
    10621 gggttgctta gcggaaatcc cggagaatat tactattctt tgcaacttcc accatttgca
    10681 ccacctagct ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct
    10741 ctttatttgt tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt
    10801 gtgattcagt tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt
    10861 ttcattgcag caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag
    10921 ttgtggctac atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta
    10981 ttcgcaactc tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc
    11041 ctgtaattta acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg
    11101 gcactcttta ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta
    11161 ttctggtcat aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta
    11221 tcttgacttg gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga
    11281 tcttggtact gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta
    11341 ttcgatccat caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa
    11401 gcattctcat ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc
    11461 agttcatttt ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg
    11521 atcatttgtc cttcgccttg caactggtct tgtttgattg tttcaaacgc aggggtgtag
    11581 ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact
    11641 gcttttaaaa tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt
    11701 ttcgtcacta cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt
    11761 cgcattcgag aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca
    11821 tggacaccat ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg
    11881 atcaaatctt ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc
    11941 agttgctcaa ttgaatccat ggccatttcg acaccaccaa atgcataggc atgatttaat
    12001 ttatctaccg ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc
    12061 gttgattttt tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt
    12121 tccgttcttc ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg
    12181 tcatcaatgc ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt
    12241 ttgtttactg tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca
    12301 atcagcaaaa caaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata
    12361 cacctctgtt tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca
    12421 ctttttaatc ataagaaaac tttaaaaaac atttttgtgt aacgattact attttttaaa
    12481 atttctcaaa attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca
    12541 cgcagttcgc aatcgtttta tcttttttta cgtatttttt ccagaaaaag cgaacaacaa
    12601 ggtattttcc aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac
    12661 agtctttgcc taccgttcag attatcattc gatttcacga aaatttcttc ctatgactgg
    12721 tccttttgtt aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa
    12781 atgactcgaa ctagtgattt cattaccatc atcttttaat ggatagatat gctcctcttc
    12841 aaagtaaaac gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta
    12901 gtggtttcac aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat
    12961 tactaacgaa gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat
    13021 gcatttactt agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct
    13081 aagaagtcgt gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt
    13141 ccgcgtgcac cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag
    13201 ccgtctcctt gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata
    13261 aacccaaatc ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatag
    13321 cctccaaaaa agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc
    13381 tagcaagctt tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa
    13441 tggttatttt gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa
    13501 tgctgcaata taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac
    13561 gtccaaacct agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga
    13621 ggtaatagct aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc
    13681 agcggctgct tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat
    13741 cgcttctttt atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa
    13801 aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg
    13861 aatagcgtcc atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt
    13921 ctcaattgct gcatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg
    13981 catcgtttct tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa
    14041 tgtgtaagtc ataaaaaaat tcctcctcaa tgttttcttt ataatgtcta cattacaaag
    14101 agtaacaaag agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc
    14161 tacttcttct tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt
    14221 cgctaggata acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac
    14281 attgatcaac aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa
    14341 gaacttaccg cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca
    14401 aagtttgttc gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa
    14461 ggccatactt aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt
    14521 agccaaacca ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata
    14581 tccttttaac tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta
    14641 tcatactcta tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc
    14701 tttgcctttg tctctgcttg gatc
//
LOCUS       DACCWK010000059        13875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCWK010000059 DACCWK010000000
VERSION     DACCWK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.059"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            9..731
                     /locus_tag="IVW65_001775"
     CDS             9..731
                     /locus_tag="IVW65_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7900649.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="IVW65_001776"
     CDS             789..2828
                     /locus_tag="IVW65_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7900650.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="IVW65_001777"
     CDS             complement(2937..4088)
                     /locus_tag="IVW65_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7900651.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="IVW65_001778"
     CDS             complement(4242..4772)
                     /locus_tag="IVW65_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900652.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="IVW65_001779"
     CDS             complement(4871..5221)
                     /locus_tag="IVW65_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7900653.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="IVW65_001780"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="IVW65_001780"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7900654.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="IVW65_001781"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="IVW65_001781"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7900655.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="IVW65_001782"
     CDS             complement(7624..8286)
                     /locus_tag="IVW65_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7900656.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="IVW65_001783"
     CDS             8470..10116
                     /locus_tag="IVW65_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7900657.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..11189
                     /gene="uxuA"
                     /locus_tag="IVW65_001784"
     CDS             10107..11189
                     /gene="uxuA"
                     /locus_tag="IVW65_001784"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7900658.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11297..12850)
                     /locus_tag="IVW65_001785"
     CDS             complement(11297..12850)
                     /locus_tag="IVW65_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7900659.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12998..>13875
                     /locus_tag="IVW65_001786"
     CDS             12998..>13875
                     /locus_tag="IVW65_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7900660.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNP"
BASE COUNT         4708 a         2576 c         2564 g         4027 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    10261 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    10321 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    10381 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    10441 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    10501 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    10561 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    10621 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    10681 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    10741 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    10801 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    10861 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    10921 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    10981 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    11041 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    11101 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    11161 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    11221 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    11281 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    11341 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    11401 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    11461 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    11521 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    11581 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    11641 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    11701 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
    11761 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    11821 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    11881 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
    11941 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    12001 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    12061 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
    12121 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    12181 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    12241 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    12301 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    12361 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
    12421 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    12481 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    12541 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    12601 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    12661 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    12721 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    12781 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
    12841 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    12901 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    12961 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    13021 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    13081 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    13141 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    13201 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
    13261 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
    13321 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    13381 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
    13441 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
    13501 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    13561 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    13621 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
    13681 agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
    13741 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagcccc
    13801 agaagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc aaacagtacc
    13861 ggaaacaaat ccaga
//
LOCUS       DACCWK010000060        13807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCWK010000060 DACCWK010000000
VERSION     DACCWK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.060"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVW65_001787"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVW65_001787"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7900661.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IVW65_001788"
     CDS             complement(2260..2511)
                     /locus_tag="IVW65_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7900662.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IVW65_001789"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IVW65_001789"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7900663.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IVW65_001790"
     CDS             3461..3679
                     /locus_tag="IVW65_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900664.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IVW65_001791"
     CDS             complement(3968..4480)
                     /locus_tag="IVW65_001791"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7900665.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IVW65_001792"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IVW65_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7900666.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IVW65_001793"
     CDS             complement(6933..7367)
                     /locus_tag="IVW65_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7900667.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IVW65_001794"
     CDS             complement(7377..8165)
                     /locus_tag="IVW65_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7900668.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IVW65_001795"
     CDS             complement(8162..9103)
                     /locus_tag="IVW65_001795"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7900669.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IVW65_001796"
     CDS             complement(9280..9867)
                     /locus_tag="IVW65_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7900670.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IVW65_001797"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IVW65_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7900671.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IVW65_001798"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IVW65_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7900672.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IVW65_001799"
     CDS             complement(12784..13719)
                     /locus_tag="IVW65_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7900673.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACCWK010000061        13722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCWK010000061 DACCWK010000000
VERSION     DACCWK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.061"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            285..1091
                     /gene="proB"
                     /locus_tag="IVW65_001800"
     CDS             285..1091
                     /gene="proB"
                     /locus_tag="IVW65_001800"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7900674.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1088..2335
                     /locus_tag="IVW65_001801"
     CDS             1088..2335
                     /locus_tag="IVW65_001801"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7900675.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2411..2755
                     /locus_tag="IVW65_001802"
     CDS             2411..2755
                     /locus_tag="IVW65_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900676.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2872..3198)
                     /locus_tag="IVW65_001803"
     CDS             complement(2872..3198)
                     /locus_tag="IVW65_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900677.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3813..5015)
                     /locus_tag="IVW65_001804"
     CDS             complement(3813..5015)
                     /locus_tag="IVW65_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7900678.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5132..5647
                     /locus_tag="IVW65_001805"
     CDS             5132..5647
                     /locus_tag="IVW65_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7900679.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5846..6292)
                     /locus_tag="IVW65_001806"
     CDS             complement(5846..6292)
                     /locus_tag="IVW65_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7900680.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6339..7817)
                     /gene="msr"
                     /locus_tag="IVW65_001807"
     CDS             complement(6339..7817)
                     /gene="msr"
                     /locus_tag="IVW65_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7900681.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8171..8800)
                     /locus_tag="IVW65_001808"
     CDS             complement(8171..8800)
                     /locus_tag="IVW65_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7900682.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8784..9248)
                     /locus_tag="IVW65_001809"
     CDS             complement(8784..9248)
                     /locus_tag="IVW65_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7900683.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9735..11003)
                     /locus_tag="IVW65_001810"
     CDS             complement(9735..11003)
                     /locus_tag="IVW65_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7900684.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11234..11971)
                     /locus_tag="IVW65_001811"
     CDS             complement(11234..11971)
                     /locus_tag="IVW65_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7900685.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(12038..12607)
                     /locus_tag="IVW65_001812"
     CDS             complement(12038..12607)
                     /locus_tag="IVW65_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7900686.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12735..13607
                     /locus_tag="IVW65_001813"
     CDS             12735..13607
                     /locus_tag="IVW65_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7900687.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         4142 a         2817 c         2474 g         4289 t
ORIGIN      
        1 gtgcgcttca aaacatatta ctcaataaag aaaatcttaa atttcatctt gacttatcac
       61 cattagacta aataaactac tcctattcta acatatccga ggctgtaaaa gactttcttt
      121 acaaattctt caagaaattc tgctatctta aagtaaacca ttaattcgtt ctttttccaa
      181 acgactgcaa gttgtacaaa aaaactattt tttacatgac tttactcgtc aaaacaagta
      241 gaatagaacg aaattaatga gtggatattt gattgaggtg tcacatgcgt aaagaaatta
      301 aaaatgcaaa acgaattatt gtcaaagtag gaacaagtac attgatttat cccaatggaa
      361 atatcaatct tgcggcaatc gatcagttag cttttgtcct ttctgatctt agaaatcggg
      421 gaaaggaagt gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat aaattacatt
      481 tgtcagaacg tccgactttg attcctgaac agcaagctgc ggcggctgtt ggtcaagccg
      541 aactgatgaa tatctataat caaagattca gtgtatataa tcaacagacg gcacagatcc
      601 tcgtgacaag agacgtggta gattatcctg aaagtcgtaa aaatgtgagg aatacattgg
      661 aacagctttt gattatggac acgatcccta tcgtgaacga aaacgatact gtagcagtgg
      721 atgaattaga tcatctgaca aaatttggcg ataatgatca gctgtcagcc atcatggcaa
      781 aacttgtcgc ggcagacctt ttgatcgtcc tttctgatat cgacgggttt tattcagata
      841 atccgactac aaatcccgag gctcagatgt atcgctatat cgataaaatc gatgaaaaga
      901 ttctggcaca agcaggtggt gctggtagca catttggaac aggcggtatg cagagtaaat
      961 taaaagccgc ttctaggata tttgaagcaa acagcgcaat gattttagca aatggaaaaa
     1021 atccggcaat tatttttgat attcttgcag gaaaagaagt aggtacttta tttaaggagg
     1081 acttgaaatg actgacttga tgcaattagg acaacaggca aaagatagcg cgcaacaact
     1141 agcacttatg cctacaaacc gcaaaaatga gctacttgtt caaatggcac aaaccatcaa
     1201 aaagaatcaa caagcgatca ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa
     1261 caatatcagt gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa tcaatgccat
     1321 ggctacagag atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag tagatgagat
     1381 gtggacgaac accgacggat tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg
     1441 catcatttac gaatctcgtc caaatgtcac gacagatgca gctagtctag cattcaaatc
     1501 agggaatgcc gttatccttc gcggtggcaa agaagcattc ttctcgaatc aattattagt
     1561 ccaattattg cagcaagtac tggtcagtgc tggcgaatct ccttatgcca tccagtttgt
     1621 cgatgatact tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat acttagatgt
     1681 gctgattcct cgaggcggag caaaattaat ccaacgagtc aaagaacagg caacggtacc
     1741 tatcattgaa acaggtacag gaaataatca cgtatatatc gataaagaag cacagttatc
     1801 tatggcagtg aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc
     1861 agaaacattg ctgatccatt ctgagatcgc accttttttc ttgccagcta ttgaaaaaga
     1921 actggtagaa cacggggtct ctttacgtgc ggatgctcgt gcgttagaat atctagagac
     1981 ggctgcttta gcagaagaca cagattggga tactgaatat ctagattata tccttgctgt
     2041 caaagtggtc gattctttgg atgaagcaat tgcacatatc aatcggcata atactaaaca
     2101 ttcagaaacg atcgtgacag atagttatgc ggctagtcaa cgtttcttaa atgaagtgga
     2161 cgcggcggct gtctatgtca atgcctccac tcgttttaca gacggatcgg tatttggttt
     2221 tggtgcagag attggtatct ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa
     2281 tgaattaact tccacaaaat atatcattta tggcgatgga caaatccgcg aataatgttt
     2341 cgtgtgaaac attattcccg ccttttgcct tttttctgta aaggttctat ccaactagaa
     2401 aggtgtcaat atgtttgaaa ttttaatgat tttttttatt taccttattt ctctgaatat
     2461 cgctgctttt ctgggagtcg gtattttatc ccttttcttt caattcaaaa aacgaagcat
     2521 agggagtcaa cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt
     2581 tacacgcttg cacattagtt acatggttgc attatgctta ttagcgacaa tcaattacta
     2641 ttcttttttc gatcattcaa tcgcttatac gattacgcta ctgattgctg ggatttttca
     2701 cttatcttat aaatatcagc tgaataaaaa ccacttgaat cggactttcc gctaaaaaat
     2761 atccgtacga taagtagagt gccttgaaga tcggcaaaaa actgccggac tttttagagg
     2821 ctgtgacaaa gattttgtca cagcctcttt ttccatataa acggcgttcg cctaccagct
     2881 gcctggtaaa tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc
     2941 ataataacga agcagctcat ccatggacaa gttcatcgtt ttttcttttt cttcttccgc
     3001 tgattcagcc agtagctgtt cccgatacac ctgatacata ccagctattt tttgctgcat
     3061 cgcttttcta gtttcacctt ccgcattgat tattggcaat tctttgatat agccacgata
     3121 aatcaaccga tcatctgaaa attcaatggg taattcaacg agtgtcaacc cgacttcctg
     3181 aaaatattct tccagcatct attatccccc cattcttttt cttaaatgaa ttttagctta
     3241 cctactgaaa attttccagt ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg
     3301 tgtcacatcc cctttttccg aataaatggt gttcttctgc caactcctca atcataagtc
     3361 acaatattca acaaacatga gaaactgttt tttgaaattg ttccttgtga ttcagagtta
     3421 aacgacagtc tcacaacctc ttggtaaacg gcgttcaatc agctgaccct cggtattagc
     3481 tcaaaaaacg gtaaaatatg agaagctatt tttccgtttt gtttctctaa tacttaggat
     3541 aaaacgctga ttttacaacc tctgttaaac ggtgttcaat cagctgtcct tcggtattag
     3601 ctcaaaaaac ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggac
     3661 aaaacgctga ttttacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat
     3721 aagcccaacc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tatgacttga
     3781 tgcaggacag ctttttcaca acctctgatt tcttattctt cttcatttgt agcaaaataa
     3841 ttacggtgat aaatgataca acatacactg ataagaacaa tggcacctgc aaaataaaac
     3901 gcatagcttt caccaatatg ttcaccaatc acacctgcaa taaatggtgc taaagctcca
     3961 ccgaaaaagc ggacaaaact atagctggac gaagcaacgt ttcgttgcag acctggtatt
     4021 tccatagcaa tcgtcgtcaa cagtgtattg ataattcctt ggaaaaatcc tgctaacaca
     4081 atgctgatcg gtacaaggac tggcatagaa gttccagctc ctaataacag caaacaaata
     4141 atgaataaga caaatgctgc taaaattgtc cgataagtcg taaacttccg ctcaagagcc
     4201 ggtgcaacga aaatcgaagc aatagccaga agcacacccc agccaaaaaa tacaaaaccg
     4261 acttctaatt cagaatagcc aatcaataaa aatggcgcat acgcaagtaa agtaaagaat
     4321 ccgaaattat ataacaatgc gattagtcca actgaacgaa gtttatgatt gcttaacgct
     4381 ttgacacctt cccaaaggcc tacttttctc tttggtttct cagtttcagg taaaagtacc
     4441 gctagggcta taaaaccgca aaacatcaaa aatgctaccc caaaaaatgg ataacgccat
     4501 gaaccactgc ccaagacccc accgactaga gggccaacag ccattccgca gccaagtgct
     4561 gcttcataca tcatgattgc tttttctgtg ttaccaacta aaatactgac gatcgttgct
     4621 aaagcagtag agacaaataa agcattcccg acaccccagc cggcacgtaa cccaatcagc
     4681 attccgatat tcccagataa tccgccaaga ccagcgaata caacgatgat aaacaatcct
     4741 gtcatcaatg tttttttcaa accgatccga ctagataaga aacctgtaaa tagcatcacc
     4801 acaccagtga ctaacatgta actagtaaat aacagggtcg tttcagctgg cgtagcgttt
     4861 aactgtgcag caatcgtttt taagattggg tctactagac ccactcccat aaaagcgatc
     4921 atacaggtag ctgctacaac tagcgctgaa cttttcccct ttgtttcttc atttgtttgt
     4981 tcttgtatat ctatttgatt ctccaatatt tccatccctt tctctctatt agcatcatac
     5041 atgatttatt ttacatgtca atatgcacat gtaaaacgtg gaaactttac tgtgatataa
     5101 taaagaaaat aagacgagag gtaggatttt tatgaatact ttagcctata tattattatg
     5161 cgtgctttta tccaaaccac gttctgggta tgaattgaag cagctgatca caattttttg
     5221 ggaagcccat cacagtcaga tctatacaac gcttgccaag ttagaccagc agggttatgt
     5281 gaccgttttg gacaaagacg agcactctca aaaaaagatc taccacttga ctgatgaagg
     5341 acgtgctctt gtagaggaat ggatcaaaga ggaaactccc gctcctacgc aaaaagatga
     5401 atttctagca aaaatctatg ctttttcgac gctcgataaa acaacagcta ctggactgct
     5461 attcactaga gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga
     5521 cctctcgcta gacaagaaac aagaatttgg acgttatgtt gtgatccagc gcagaattat
     5581 gctttgtcag caggaactcc aatggtgtca gcaagtaaga gaacaaatgc agcagttttt
     5641 tgagtaaggg ataaacagaa cctttctaga aatatctatt gaagagcaaa ttatcttcaa
     5701 taaaagagca atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg
     5761 ttcggatcac tatttttcta gaactgcatt caagagacta tgacaaaaag catgccaagg
     5821 ccttttttca aataacagtg ttccatcatt cattactttg aattgctttt agcacacttc
     5881 ttgaaagcgg gccaacgatg atcaactgca acggtaaagc ggcaatcaca ttcattttcc
     5941 aagcattcat ataagcagag aataattgat caggtattcc ttgttccatg atcacaccga
     6001 ataaagacat acatgtcacc attcctaaaa ccatcaagca tgaaatagtt aagatcattt
     6061 ggaccttgct ttctggattt actggtagtt taaaggcaat ttttttcgcc agcacaccga
     6121 tgatgaagac atccaaaatg aaagccacca cgaatccagg cactagacca atcaacaaac
     6181 ttgataaatg aagatcttga tgcagccata aattatacgc gctcatcccc aagaccatca
     6241 aaaaacacat cattgttgta aaaattattc cttctttttt atttgtaggc aatctgtacc
     6301 actcctttaa atttgtcacg aaaatcatcg tagcacattt aaaaatttct cgtaagtact
     6361 ttttttattt tctgcccttt tatttcataa acttcatcag ccgtacgctc gacaaagtaa
     6421 gaatcatgag aagtaaacaa aatcgttccc ggatatattt gcattagctt ctctaaagct
     6481 tcgatcgttg ccatatcaat aaaattagtc ggttcatcta acagcaacac attacttggc
     6541 ttagtaaaaa gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa tgtacataac
     6601 ggtttcgtca aagtttcctg agcaaaccct aagtgattca gcaaactacg gacaagcgat
     6661 tctgaaaact ctgtttgtct catgacaaaa cgaatgatgg attcttcaga tgtcatttga
     6721 taaccctttt gctgatatac ttgaaagctt actttcggag agagaaggat tccttctcct
     6781 tgtttgcgga tatgttctaa taaggttgtc ttacctgaac cattttcacc gacaatcgcg
     6841 atttttttac ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct cccacttctt
     6901 tccaattgga cattttgtcc catgatggga tagcggctgt ggagttcaag agattttggt
     6961 actggaaaac ggatttgttt catctgctgc ggcttggttg tttctcctat tttttggagt
     7021 cttctctcta atgttttcgc attcttttgg atggcttttt gtacactgtc tttttgttta
     7081 gaggaagaca accgatccag tctgatttgt tgctttttct ttttactgga cactttttcc
     7141 aacttttccg cttgtttttt cttttcttgg atcgattttt ccaatcgttt tttctctttc
     7201 atgatcgact ctgcttctcg tagctgagtc tttttctcca attccttttg agtgagatag
     7261 gcactataat tccccgaaaa ctctcggatt tctccgtctt ttacttccca gattttcgat
     7321 gctagctcat ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc ataataataa
     7381 cgaagctctt caatcagtcg atcaatactt tttctatcta gatgggttgt tggctcgtct
     7441 agaagcatcc cttgttcata agaagaaaga agacgtatca actcgatctt tcgttcttcc
     7501 cctccgctcc atttccgctc tgaagtttct tccagtctta actggaaatg actggctaat
     7561 tccaaattta ggtcctttgc ttcagcgaga taggttaatt gaggcaggta gttgaaggtg
     7621 atctctgttt ggattttccc atgatcggga aaaagttcac cggctattag tttgagcaaa
     7681 gtcgatttac cgacaccatt ttctcctatg atccctatac gatcttgctg atagacacga
     7741 agagaatcaa tagatagttc tagctggttg ccaaagctga cttgtagatt tgttatattt
     7801 actgctaaat tttccatatg ttttacactc ctatttcatg taaaacaaca attatcgata
     7861 ctaaaaaaga gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt tcctactctg
     7921 aacgaaataa ctatgactaa atcaccaaat ctgtcatagc tttcacctat tctactgtcg
     7981 ttcgaaaaaa tggacatact ccatccatga aattgttgtt tatacatgtc ttttgaattt
     8041 atttagttgt tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc
     8101 tcctttttta ttaggttacc acacacgatt caaaaaaagc aatagctact gaatagtaac
     8161 tgaaaacctt ttattcgaac atagtgatct tttctggtgc taaacgaaca gactcgtaac
     8221 cttcttctac cgcttttcct attgataaaa taataactgg aacataacgg tctttatcca
     8281 aaccaaatgc ctctgccaac tgatctgctt cgaatccacc aattggattt gtttcatagc
     8341 catgatcacg cgcaactaac atgaattgca tagccgctaa gctagaatcg actttgacta
     8401 catcgttcat ttcttctctt gaaaaactct tgtaataagg aatgatcgca gctaattgtt
     8461 gatctctgac ttcttttggc atcttccctg attcgtaagc ttggttgtag atttcttcgc
     8521 catattcgta acattgcata tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt
     8581 gtctcgtatt gaaacggatc aaaggacgca agatttcttt tgcttcctcg ctttcagcga
     8641 ccacaaagcg ccatggtttc atattgacag aagaaggtgc agtcgtcgct tctttgatca
     8701 tttccagcat ttcttcgtgt gggattttgt atgtttcatc atatacgcga acggatcttc
     8761 taccagtcat aattgttgaa aaatcatttt tttgttccat tattattaac ctctttcgtt
     8821 tctgttgtta tattgcaaag ttgggtattc aatttatcta aaagtgttag aaattgtttc
     8881 atttctacat cgctgaaagc cggggatagc acggaacctg tatctttatg gtccatttca
     8941 caatgcttga ttttcttctg ggctttttct gttagcgcta catacacttc ccggttgttc
     9001 tgaggatttc tctgacgcga aacatagcca tttgcttcca ataccttcag atgacgagta
     9061 actgctcctt gatcaatctg caacttttct tgcaaactgc tttgtagcaa tggattttct
     9121 tctttcagtc tcatcatgat ctcgtatcta gtaagactta taccgatatt ttcttcaaag
     9181 atctggacga ttttttgatc ggctaatttg atttgatata aaaggtgact aatctcttct
     9241 aaagacataa tttcctcctt tctaataatt gactcgtcaa cgattgactt gtcaataata
     9301 gaataaaaaa agaaaaaagt caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag
     9361 aaaagtatag agaaaagaaa ttttttgtag aatgaacgga aaaaatgata aagaaagcac
     9421 aacgctaaaa ccaagattat tgattcttat caaactatag aagatagctc ttctgcagta
     9481 gataatttta caccgccaaa aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat
     9541 tagaaattgg atcagtatgc ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca
     9601 accttttttg agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac
     9661 aaaaaacatg aaaatctgtt tttgtttggc tgttcttact acttgatgca gggcagcttt
     9721 ttcactacct ctttttaata accataaaat cctattcctt catatctcca gcccatcggt
     9781 gttagccaat ctctttcctt agcactcaag gtatagtgat gggtaccagt tttttcattt
     9841 ggattaaata gtcgataaat tggcttggaa ccaccagaat aaaaacttag accttcataa
     9901 cgccaaccga tttttgttaa agagttgatc tcagattgtg ccattgtgta atgatggtct
     9961 ccactgtaag gattgtataa acggtagaca ggttgtccgc tgtttggtgc ttgccaagca
    10021 atcccttcat attcccagcc tacagttggc agataatttt tttctccggc attcaatgta
    10081 tataaatgtt ctcctgtatg acggttatac agacgataca tcggagaagt tgaagttggt
    10141 gctgtatcat actgtgtcag attataggtt tcgattacat tgttcaatgt agaaccataa
    10201 ttaggcgcag tggcataacg acctgttagc caagccgtag catctttgta agagttcgta
    10261 ttgcttttcc atgcaccgga atagtagtaa acgcctggct gaaaagaagt cgtcttcaat
    10321 accgttacgt tatcttgcaa tgattctttg tacgaaggat attttctaaa agcggcattc
    10381 accgtaatcc attgcccatt cacatattcc gaagtaggca tcgtaacaga ttggccatta
    10441 tagcttcctt taatcccaaa tagattgtaa ttaggggcag aagccaaagt actgttcccc
    10501 cagccacttt caacgatggc ttgagcaatc atgactgatg catataagtc attggcactt
    10561 gccaaacttt gtgcagttga ggcaatacta tcaatgaacg cttgttgaga attagcagat
    10621 gagcgagcat aaaaagcata gccagtatag ttttcgaatt cagacaaatc tgcttgatgg
    10681 gtatgtgtca atgtttgccc atctgtcaat tggttcgtga tttcttctgc attctctgga
    10741 ttgttctgag cttctgctga tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt
    10801 gttggttctt cggttgaaat tgtattttct gagacagtag ctgactgcaa agtactttct
    10861 gtatcgtttt caattcctgt ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc
    10921 gattcttcag ctagcactgt tgtgttggca actgctagat ttcctaaaag aataacgcta
    10981 aataataaat tctttgcttt catctttttt ctcctttgaa ttatgaaact tttcctaaat
    11041 aacagtgtaa ctaaaaaagt aaaagcaagt caatgtccat atttaaataa catttgtttc
    11101 tttttaataa atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat
    11161 tcacgcctta aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat
    11221 tatccgttat ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca
    11281 tccggtctgg cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc
    11341 cccattcttt ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat
    11401 ataattcagt ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt
    11461 gctcttgacg taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg
    11521 cggaatcagg ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt
    11581 tttgtttttc catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga
    11641 tatcaagcat attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga
    11701 ttcctgcatt attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa
    11761 ctgcttggac ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg
    11821 gtaaagattc ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag
    11881 cccctttttc tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga
    11941 tgacaatcac tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc
    12001 ttgcttatac tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt
    12061 tgacgatttt ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag
    12121 ttgcattcgc tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc
    12181 ctgctccgat ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt
    12241 cttttggatc ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg
    12301 aatcttctaa acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat
    12361 ttttccctat actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg
    12421 tatcatctac tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat
    12481 ggtacccagt attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca
    12541 cttcaaagag cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc
    12601 ttgtcatttt gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat
    12661 acttataaaa catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa
    12721 acaggaggga aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa
    12781 aaaatatcag ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac
    12841 tgaaagattt agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac
    12901 agttgacgga ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag
    12961 aaaaaacggc gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg
    13021 gaggcgaaac taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc
    13081 atcctgatat ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg
    13141 atagcggatt acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt
    13201 catttcgact gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag
    13261 caaagcaaac agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc
    13321 aaacgtcagt ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga
    13381 tcgtcggaac atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg
    13441 gtgcattagc tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac
    13501 tcgatccgcc attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc
    13561 ctgctgctcg taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt
    13621 ccttcgcttt cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa
    13681 cataagtttc ttaatttgtt ccttcctttt atttctgata at
//
LOCUS       DACCWK010000062        13583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCWK010000062 DACCWK010000000
VERSION     DACCWK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.062"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            39..818
                     /locus_tag="IVW65_001814"
     CDS             39..818
                     /locus_tag="IVW65_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900688.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            864..1808
                     /locus_tag="IVW65_001815"
     CDS             864..1808
                     /locus_tag="IVW65_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7900689.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1824..2603
                     /locus_tag="IVW65_001816"
     CDS             1824..2603
                     /locus_tag="IVW65_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7900690.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSHSRV"
     gene            2615..3313
                     /locus_tag="IVW65_001817"
     CDS             2615..3313
                     /locus_tag="IVW65_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7900691.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3341..4105
                     /locus_tag="IVW65_001818"
     CDS             3341..4105
                     /locus_tag="IVW65_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7900692.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            4119..5936
                     /locus_tag="IVW65_001819"
     CDS             4119..5936
                     /locus_tag="IVW65_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7900693.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            5990..6913
                     /locus_tag="IVW65_001820"
     CDS             5990..6913
                     /locus_tag="IVW65_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAP7900694.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKVSES
                     NYIEELIKVIKFFEIKLIVPTIDTELSTLSRNKKYIENETDSVVLVSSEKVIDICENK
                     ILTSAFFEQNGFKTPEILNSSEITKFPVFIKPLNGSSSVNTFIVNNEKELLFFSEYID
                     NPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKK
                     MLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKN
                     LRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            6885..7601
                     /locus_tag="IVW65_001821"
     CDS             6885..7601
                     /locus_tag="IVW65_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7900695.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            7598..8716
                     /locus_tag="IVW65_001822"
     CDS             7598..8716
                     /locus_tag="IVW65_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7900696.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            8709..9326
                     /locus_tag="IVW65_001823"
     CDS             8709..9326
                     /locus_tag="IVW65_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7900697.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            9453..10622
                     /locus_tag="IVW65_001824"
     CDS             9453..10622
                     /locus_tag="IVW65_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7900698.1"
                     /translation="MCRVLNILFQYEKIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNY
                     CNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFL
                     YRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVH
                     FLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYH
                     GYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGY
                     LCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            10629..11024
                     /locus_tag="IVW65_001825"
     CDS             10629..11024
                     /locus_tag="IVW65_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7900699.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            11021..12022
                     /locus_tag="IVW65_001826"
     CDS             11021..12022
                     /locus_tag="IVW65_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7900700.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNLSDEYYTRAICKCKYKDNWKGISAKFALKNKWVFLIYIYNKLIKR"
     gene            12028..13074
                     /locus_tag="IVW65_001827"
     CDS             12028..13074
                     /locus_tag="IVW65_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7900701.1"
                     /translation="MSVKISVIVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTD
                     NTLAILKKYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFES
                     LYNCAIAENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNR
                     LYSHNAWDKIYRRSLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYR
                     QQNNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQK
                     NVGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKM
                     YKTAYNMLWRIYNR"
     gene            13088..>13583
                     /locus_tag="IVW65_001828"
     CDS             13088..>13583
                     /locus_tag="IVW65_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7900702.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIP"
BASE COUNT         5067 a         1956 c         2669 g         3891 t
ORIGIN      
        1 tcttaattta tctatcatca atacttagga gtgaatctat ggaaaagcca ttcgtcaagt
       61 cattgactgc tgtggcggta ggtgcaggtg ctattgcgat ctgtttgttt ggttaccata
      121 tcaataatca gcggcaacat cagcaacgga tcaattatgc agaatctgcc atcaccaatc
      181 aaaaagacac cttgacttct ttatcaaaag aagtagacaa gctgtatagt acaaaagaaa
      241 aaaattttct aaaccctgaa ataacagaag aaacaatcac aagtttgtca aataagctat
      301 cctctgtcaa actttcagca gatgattacg acatcaaaga atcagaattg cctaaagaag
      361 ctgttgcaat ccaagatgag aagaaagcaa tcgtgacaca gttagatagt gcagaatcaa
      421 aactgaagat ccagacagct gtcaacgatt tatttactaa aaatgtatca aactggcagc
      481 aagctgtaga cgatgtcgtc atcaaagaaa aggtgacaag tgcagatgtc gctcgtgtac
      541 gtgaaaatat gagctttttt aaagatagtc cttggaaaac ggttgttatg caatatctag
      601 gattcgctga tactcagatc gcgcaagtca cgcaactaga tcagttattc gatacgatgt
      661 tgaaggatgg tcaagtgact gccaccgcta cttacgatca atatttgacg gcgctcagtc
      721 aaatagagca gatccggaat gaaaaaataa gcgcagcata cgcaacaaaa gcacaaactg
      781 ttgctcagca aatgggctat agcaatataa gttattgaca gttaaaacgt tgattcaagc
      841 aaggttagca aacggaggaa actatggcaa gacgaagaag aaaatcaaga gggaaaaaaa
      901 tatttctgat tattttagca gttcttatcg tattagtatt agcaattgta ggagtaggag
      961 caaaattata tctggatctc tcacattcta ttgagaaaac ttacgaatca gtggaacgaa
     1021 cccaaggaga acgaacgaaa ccagttgatc tggacaaaca agaatctttc tctgttctat
     1081 tgatgggcat tgatactgga gatttaggtc gggtcgatca aggaagatca gatacgatga
     1141 tggttgccac agtcagtcct gaagacaaac agacaacgat cgtcagcatt cctcgagata
     1201 cgtatgtcga catcgtaggt cgtgacacaa aagacaagat caaccacgct tacgcattcg
     1261 gcggagctgc catggcgatg gataccctac aaaaatatgt agacatccca atcgatcact
     1321 atgtctctat caatatgaaa gggctaaagg aactcgttga tgcagttggt ggtatcgaag
     1381 tcaataatga cctcactttc tcacaagatg gctatgactt tccaatcggt aaaattacat
     1441 tgaacgggga tcaggcatta tcttattctc gtatgcgtta tgaagatcca aatggtgact
     1501 atggtagaca agagcgtcaa agaaagatcg tagaaggtgt agcaaaaaaa gtattgagtt
     1561 taaacggtgt cagcaactat caatccgttt tgaatgctct agagtcgaat atgaaaacag
     1621 acatgagctt tgatgaaatg aaaaaaatcg ctttagatta tcgggaggca tttggtacga
     1681 tcaagcaaga tcagctgcaa ggagaaggct tcatgcaaga taatatctcc tatcaaaaag
     1741 tcagcgatca agaacttgat cgtgtgaaaa aagaattaaa agatcaaatg aatctagaga
     1801 ataaatagga aagtggagaa aaaatggaag agacaatcag cttgaaagaa atttttgaaa
     1861 cattacgcaa acacttggta acaatcatca tctcaatgtt tgctggcttg gccatctcag
     1921 gaatcgtaac atttttcatc ttgacaccaa aatacagttc acaagcacaa ttgatcgtga
     1981 cactgccaca atcagacaca gcaaatgtca atgatgtcaa tacgaacctg caaatgatca
     2041 atacgtataa agatatgatc gtcagtgatt tagtactaaa tgaagtcaaa gatcgtttag
     2101 aaacagaaga caatgtgaaa atgactgctg gacaaatcaa agatgcgatc tccgtcaatc
     2161 agtcacaaaa ttcacaaatg ttctcgatcc aggcaataag cacgaatgca gtgactgctc
     2221 aacagatcgc taacacaact tcacaagttt tccaagagaa cgcaaaagac gtgatgaatg
     2281 tcgataaaat ctcgattatt tcaggtgcag ttgccaatac gacacctgtt tcgccaaata
     2341 ataaattgaa tctagtgatt ggcttagtat taggaatgat ggtaggcgta ggtctggcat
     2401 tcttattcga attacttgat cggacagtga aagatgaaaa atatgtgacg gatgcattag
     2461 gattcccaat cttaggaaca gttccagaaa tgtcagcgaa agaattgaac gctggtatcc
     2521 gtaaaaaagg aacagctcct gcacgaccaa aagctacaaa cgataaaaca gccccatcac
     2581 gcagaagcca ctcaagagtc taggaggaag aaaaatggca cgaacacaga aacaaaaagc
     2641 agcacaaact catgcagtca gtttgatcac attaacaaac ccatcttcac caatcgccga
     2701 acaatatcgt acgatccgga cgaatatcca gtttgcctcc gcagctggtc agcaaatcaa
     2761 aacgattgtc gtgacttcat caggtcctgg agaaggaaaa tccaccactg cagcaaacat
     2821 tgcagtcgtt tttgctaaat ccggtcaacg agttctacta gtcgatgcag atttacgtaa
     2881 acccgtcgtc tataagacat tccagctgaa caatgccagg ggattaagta cagcacttag
     2941 ttcatcagga agtgtagcag atgtaatcca acgaacgcca gtcgagaact tgtcgatttt
     3001 gcctagtgga cctaagccac ccaacccatc agaattgtta agttcgccaa gaatggatca
     3061 gatcctagac gaagcaagac agttatttga cgtggtgata ttcgatatgc caccagtagt
     3121 agccgtaaca gatgcacaga tcatgtcttc aaaaacggat ggcactttat tggtcgtacg
     3181 tgaaaataca tcacgtaaag aatcattgaa taaagcaaaa gaactgctag agatggtaca
     3241 agcacgagtt ttaggtgttg tatacaacgg agcagaacat agcaaagatg ctggttatta
     3301 ctacggaaat taaggaataa gaagagagga gacaagagaa atgatcgatc tacattgcca
     3361 tatcctacca ggaatcgatg acggggcaga aaacttagaa gcaagtattg caatggctga
     3421 aaaagcgatt cagcaaggca tcacgcatat tttatgcaca ccacaccaca ataatggaaa
     3481 atacagcaat gaaaaatcac aagtcatttc tctagtcgct tcgttacaag ccgaattaga
     3541 gaaaagacag ttgcctttga cgttattgga aggacaagaa gtacgtatta ccggaacgct
     3601 catcgaagac atccaccgag atgaaatttt atttacagat ttagatgata catatcttct
     3661 gatcgaattt ccgacactgg aagtgccgtt atatgcagaa cggctttttt ttgagttgtt
     3721 agaactagga aagacaccag tgatcgtcca tccagaacgc aacgcgcatt ttcgcgaaga
     3781 cccgaatcat ttgcttccgt tcctagatat ggggtgtttg gcccaactta ctgcgccaag
     3841 ttatgttggg gtatttggca aagatattca gaaaacagct aaaacaatgg tcaaacataa
     3901 tctcgtgcag atgactgctt ctgatgcaca cggagtctct aagcgcacct tttacttaaa
     3961 agagtgctat gagcagattg ccaaggactt tggtaaggaa aaagtagtac agatgaagca
     4021 agttgcaaag gatctgatca acggggatga aatcagttat ccagactatg aagaggtaaa
     4081 aaagaagaag tttggtttat tttaggggga attaaaaagt gtttatatta acaagaagaa
     4141 gaaaaattgc cacattggtt tttgtggaca gcttgttatt aattgctgca aatattgcag
     4201 cagctaaatt catgaagccg tttgtatcta taccaatgga tcttatttta atctctatcg
     4261 gtttgtcgat tggtttttat ttattttatg gtagtttatt caaagttttc actcggatca
     4321 atcgctatac aaatttaaga gagatcattg caatttttag ttcactatct gcaagtgctg
     4381 cctcaagtat cctcatactg ctgttcatta atcggcgata cagtttacgg ttagttatct
     4441 ttgcttatct gctttcgctc ttattgatca tcggtagccg attgatctgg cgtatctaca
     4501 tcgaaacaaa aaatatgaga tacgtatcag cagattctgc taaaaataca ttgattgttg
     4561 gtgctggcga aggcgggcgt attttataca acagctttct tggatccaaa acggctcaag
     4621 atatccacgt tgtcgggttt gtagatgatg atccaaataa aagaaatacg tatctttcag
     4681 gaaaaaaagt attaggtgca ttaaaagata ttccggaatt gattgaaaaa tatgacattc
     4741 aaatggtaac gatcgctatc ccttccttat caagaaaaaa attacgacga atcttcgagt
     4801 tagtcgaatc cgctcgtgtg aaagtcaaca cgatgccttc tatagaagaa cttgcttcag
     4861 ggaaaattag cgtatcaaaa ttaaaaacaa ttgacgtagt ggatttactg ggaagagatg
     4921 aggtaaagct ggatattgaa tccatcaaag atcagatcac agacaaagtc attcttgtca
     4981 ccggcgcagg aggatcgatc ggttcagaaa tctgtcggca aatcattcaa ttcaatcctg
     5041 caaagctatt gcttttagga catggggaga actcgatcta cttgattgac cgtgagttac
     5101 gcacgcatca tcaaaattgt cctacagaaa tcgtaccgat catcgcagat atccaagatc
     5161 gcgagaaaat caatgagatc atggaacaat atcatccaga tatcgtctac catgcagctg
     5221 ctcataaaca tgtaccactg atggaatata atccaaaaga agcagtcaaa aacaatattt
     5281 tcggtacaaa aaatgtagca gaagcagcta aagcagcaaa agtgaaaaac ttcgtcatgg
     5341 tatcgacaga taaagctaac aatcctccaa atgtcatggg ttcaacaaaa cgaatcgctg
     5401 aaatgattgt gactggattg aatgaagaag gctgtacgaa attctcagct gtcagatttg
     5461 ggaatgtcct tggttctcgc ggtagtgtga ttccagtatt cagagaacaa attgctcaag
     5521 gtggtccaat cactgtaaca gactttcgta tgactcgata ctttatgacg attcctgaag
     5581 ctagtcgttt agttattcaa tctggcgcat tagcaaaagg tggcgaaatt tttattctag
     5641 atatgagtga accagtgaag attgtagacc tagcgaaaaa catgatccga ttgagcgggt
     5701 atagcgaaga cgaaatcgaa atcattgaaa caggtatccg tccaggggaa aaactatatg
     5761 aagaactatt gttggataag gaaagaaatg atgaagcagt ttatgagaaa atctttgtag
     5821 gaaacatcaa aggttactca atacaaaaag tgatggattt tgtcaagagc ctgccgcaag
     5881 acgatgaaca attggctaaa gacattgtca cttttgcaaa tgcttcgaat agataggaga
     5941 ttaaaaattg aatattttaa tattaagttg tggacgtcgt tgtgaactag tgaatgcatt
     6001 taaaacagca ttaaacaata atgaaatgga aggaaaaatc attggagcag attgctctga
     6061 actagcaccc gcactatata tgtgtgacga aaaaatagta ttacctaaag tttcagaaag
     6121 taattatatt gaggaattaa taaaagtaat aaagtttttt gaaattaaat taattgttcc
     6181 tactattgat actgaattat caacattatc aagaaataaa aaatatattg aaaatgaaac
     6241 agactcagtt gtattagttt cttcagaaaa agtcatcgac atttgtgaga acaaaatttt
     6301 aacttctgct ttttttgaac aaaatggttt taaaactcct gaaattttaa actcttctga
     6361 aattacaaaa tttcctgtat ttataaaacc tctgaatggt agttcatctg ttaacacatt
     6421 tattgtaaat aatgaaaaag aattgttgtt ttttagtgaa tatatagata atcctcttat
     6481 tcaagagtat attttaggtg aagaatatac tatagatgtt tgcttaaact ttgattcgac
     6541 accagtaagt attgttccta gaaaaagaat agcaactaga ggtggggaaa tactgaaggg
     6601 tcagattatt aaagatagag aactaattga gactacgaaa aagatgttgg aaaaattaaa
     6661 tgcaatcgga catattacta ttcaatgtat aaaaaacgaa aagggaatat tttatttaga
     6721 aataaatgca agatacggtg gaggagcccc cattagcatt aaatcaggag ctaattcccc
     6781 tgaatatatt ataaagatga tagattcaaa aaaaaatttg agatacagag aaaattattt
     6841 agataaaatg atagcactga gatatgatca agcgatttat ttagatgaaa aaggaaatgt
     6901 atataatgat taaagcggtg atctttgatt tagatgacac tcttgtgtct gaatatgatt
     6961 atgtaaaaag tggatacaaa tgtgtagcta gaagattagc agtgcaaaaa attttcccgg
     7021 ctacttttga cgaaatatat agagtccttt tagatctttt tgctgaagat agtaaaaatg
     7081 tatttaatcg tctatatgac tattttgaaa ttccttatag taaaaaagaa attaaagaac
     7141 tggtaaatgt atatcgagaa cacaaaccag acataaattt tttagaaaat gcagatactt
     7201 tattaacgga attaagaagt agaggataca agctggggat tattacagat gggtatgcta
     7261 taacccaacg aaacaaattg gatgctttga aagtagacta tttggtagat tatatttgta
     7321 ttactgacga attaggccgt gaattgtgga aaccacaccc tatctcatat cttttattgt
     7381 tagaaaagtt taatttaaag gcacaagagt gtatatatat tggcgataat gagttgaaag
     7441 attttataac tgccaaatca ataggaatgc aaacaattaa aatagaaaga gtaaatggta
     7501 tttacagaga aagcagtttg aagttcaaaa gattttacca agctgataaa aaaatacgtt
     7561 cgttatggga gataaaaaat tttttgggag tgatttcatg aaaagtaaaa gaattctatt
     7621 agcttctcca catatgagtg aagaaggata tgaaaaagag tatgtaaaag aagcatttga
     7681 tacaaattgg gttgctcctt taggtgcgaa tgtgaatgga tttgagaaag aattagcaga
     7741 atacgtggga gctagtcatg ctgccgcatt atcttctgga acagcagcta tacatttagc
     7801 tttgaaagcg gctggggtcg gtaaagatga catagtgatt tgtcagtcct tgacgttctc
     7861 agctactgct aatccaattc tctatcaagg agctatacct gtatttgttg atagtgatta
     7921 cgaaacttgg aatatgaaac ctgaacattt gcgaacagca ctagaaaaat atcctaacac
     7981 aaaggctgta ttagtagtac atttgtacgg tttatcagct aatttagacg aaattactaa
     8041 aatatgcaaa gaatacgatg ttactctaat tgaagatgct gcagaatcat taggaacaac
     8101 atataaagga aaatatactg gtacatttgg cgactatgga atttattctt ttaatggaaa
     8161 taagataatc acaacttcag gtggcggaat gcttgtatcc aatgacaaag aaaaggtgga
     8221 aaaagtgaga ttttgggcta cgcaatctcg tgagaaggca cgccattatc aacacaatga
     8281 aattggctat aattatcgaa tgagcaatat cgttgctggt atcggtcgag gacaattgaa
     8341 agtattaaat gaaagaattg agaaaaaacg atatattcat agtttttata aaaaagaatt
     8401 gtctaaattg tcaaatatac aattcatgcc agaaaataat tggaatgaac cgaattattg
     8461 gttaagtgtc ataacaataa agggaataaa tgcgaatcat cttattgatg agttagacaa
     8521 aaataatata gaagcccgcc cagtatggaa acctatgcat ttacaacctg tttttaaaaa
     8581 ttatgattat attggcgatg atgtagcaga aactttattt gacttaggag tatgtttacc
     8641 atcagataca aagatgacag atgaagattt aaaatatgta gtatctacga ttaaaagagt
     8701 gatcaatgat gcgtaaaaaa actttctatc agctttttgg gaaaagaatt ttagatatcc
     8761 tattgtcagg aattgctctc atcgttttat cgccgataat cttaattgta ggttttttag
     8821 taagaattaa acttggttct ccaattatct tcaaacaaga acgaccagga aaaagtgaaa
     8881 aaattttttc catgtataaa tttcgtacaa tgactgacga aagagaccac aatggagaat
     8941 atttacctga tgaaattcga ctaactaaat ttgggaaaat gcttagagca acaagtctgg
     9001 atgagctacc cgaactttgg aatattttga aaggtgatat gtcgatcgta ggacctcgtc
     9061 cattattagt ggagtatttg ccactatata gtgagaagca acggaaaaga cacaacgtaa
     9121 gaccaggatt aactggactg gctcaggtca atggaagaaa tgctattagt tgggaagaaa
     9181 agtttgattt agatgtttat tatgttgata aaattagttt ctttaatgat cttattatta
     9241 taattcagac atgtaaaaaa gtaatcaaga aagagaatat taatacaatc aataacaata
     9301 tcccagaaaa atttaaaggg agttaaatac agcagcttaa tgcaagtttt gtgacttgca
     9361 ttatatttag gtataaatca aagcgagtat attagaatta tataacatat gttaaaaaaa
     9421 cgtatagcta ataaaagtat gattttaaaa atttgtgtag agttttaaat atactgtttc
     9481 aatacgagaa aattataaaa aagtataagg aaaagagagt tatggcaaag tattataata
     9541 aaaaaattct tataatggca aacaacgatg atggtcttta ccgttttcga ggaatgctca
     9601 taaaaaactt agtagatcaa ggtaatgaag ttattgtatc tcttccaaag ggcaagaata
     9661 ttcctaaatt aattgaactt ggatgtcaaa ttattgaaac tccaatagat agaagaggaa
     9721 ttaatccaaa aactgacttt aaactatttt ttaactattg taatattttg aaaaaaataa
     9781 agccagatat ggtcattgct tatactatca aaccaaatat atacggtgga ttggcatgtc
     9841 ggttaagaaa ggtttcttat gcattaaata taaccggatt aggaacggca tttcaaaaaa
     9901 agggggtttt gaaaagtctt attgtatttt tgtatagatt agcagctaaa aaatctaagg
     9961 tcatattttt cgaaaatttt gaaaatatga aaacatttca gactttacaa attgctaaac
    10021 aaagtaaata caagctatta aacggtgcag gagtagatgt agaacacttt agttatttag
    10081 aatatcctaa agatgagcaa gttcattttt tatttattgg aagaattatg aaagagaaag
    10141 gtgtagatga actttttgaa gcaatgaaaa acttatatag tgaagagaaa aattgtgtac
    10201 ttgatgtcct tgggtatttt gaagaagatt acggcataaa aattgaaaaa tacgaaaaag
    10261 agggatggtt aaaatatcat ggatatcaat ctgatattcg tccctttgta gaaagagcac
    10321 attgctttgt tttgcctagt tggcacgaag gaatggcaaa tactaacctt gaatcagcag
    10381 catcagggag accaattatt acaagtgata tcccagggtg taaagaagca gttattgaat
    10441 cgaagtctgg ttatttatgt gaaagaaaaa atagttgtag tctctataat tcgatgaata
    10501 aatttctaaa attatcatat gagcagcgca ttaacatggg aaaaatgggg agaaattata
    10561 tgattaaaaa atttaataag aagaaagttg tcgatgaaac aattaaatca ttatttagtt
    10621 aaaaagatat gttttgcaaa gtatgtgata gaaaagaggg attctgtggc ttcagaatag
    10681 atatcaaaga attggcaaaa gtttctgata tttttttatt tactacatta caagaaggaa
    10741 tgccaagatc tatgatggaa gctatggcaa gtggattgcc atgtatagca agtaaaatcc
    10801 gaggtaatgt agacctttta gtggaaggaa aaggtggata tcttgttcca gctaaagatg
    10861 cagaattagt agcagagtgt ttgagagaac taatcaataa taaatctctt agaaagtata
    10921 tgggaaatga gaacctttcc agaattaaag aattcgaggt tagtatcgta aaaaaaagaa
    10981 tcaaagaaat atatgaagaa gttataggtg agagtgtaaa atgaaaaatc caatgttttc
    11041 aataatagta cctatctatt gcgttgaaaa atatttgaaa gattgcttag attcaataag
    11101 aaatcagaca tttaaaaatt ttgaagcaat actagttgat gacggtagtc ctgacgaatg
    11161 cccagaaata tgtgactatt attcccaaat agatggacgt ttaaaagtaa ttcataagaa
    11221 aaacggtgga ataatagatg cacggaaaac aggagtcact agtgctatgg gagagtatat
    11281 agtttttcta gatggtgatg attggcttgc aaatacttat ttagaaaaat atgcggaagt
    11341 aattactaag tacaatccag atataatatg tagtgggtat tttgcatcac gttccaacaa
    11401 aattagaaaa agagaattgt cttgctcgtt tggatattat gacaaagata aaattataaa
    11461 tttgatttat cctaaattaa ttgaggatga acatggagag tattttagac cacaattatg
    11521 ggcaaaagct tttagaacta ctctgtacaa ggaacaacaa caagtatctg ggcaagttag
    11581 tgttggtgaa gatcatgcat gtacaaaacc atgtatttac catgcaaatt cgatatatat
    11641 gatggaaaaa tgtttatatt attacagaat aaacgaaaat tctataacaa aaaaaccaca
    11701 accatattat tgggaaggac caaaggctat tggagagcat tttgaaaagc agattgatat
    11761 gaataagtat gattttcaag atcaagtata tagaaatgtt acacacaatt tatttaatgt
    11821 tgctgtttca caatttaata aacaggacaa atatttaaat ataaaaaaag atattataca
    11881 aaatctaagt gacgaatact atactagagc tatttgtaaa tgcaaatata aagataattg
    11941 gaaaggaatt agtgctaagt ttgcattgaa aaacaaatgg gtatttttaa tttatattta
    12001 taacaaattg ataaagaggt aataataatg tcagttaaga ttagtgtaat tgtaccttgt
    12061 tataatgttg aaaaatatat tgatgagtgt ataaatagtt tgataaatca aacattgaaa
    12121 gatattgaga ttattttgtt aaacgatggt tcaacagata atacgttggc gatattaaag
    12181 aaatatgaaa agcttgattc tagaataaaa gtttacgaac ataaaaatat ggggttaggg
    12241 ccaactagaa atcgagggat caaaatagct aatggagaat ttttggcttt tgtggattct
    12301 gatgattttg tttccaatac tatgtttgaa agtttatata attgtgcaat tgcagagaat
    12361 gcggacattg caattggaga agtaggcatt ttttctgata gagaaataga aagagtaaaa
    12421 ataagaaaaa gtctaacgaa cttaaaaaca attgaattag caacatataa caagaaagaa
    12481 ttttttaagc aattctattt caatcgttta tattcacata atgcttggga taagatatat
    12541 agacgtagtt tagtattaga gtttaattta tcatttggtg ataacaaaat aatttttgca
    12601 gaagataatt ggtttcaatt acaagtattt atgacaaata caagaatctg tttcgtagat
    12661 aaattgtgtt attattatcg ccaacaaaat aattctataa tgcataagcc aaaaagtaat
    12721 ttggttttta gacatggaaa tatgattgct gattatgaaa agttgataag gcaaaatgga
    12781 aacaacataa tagataaaaa agcttgtatg ttggttgcat ttgatgtact aattatggag
    12841 atttttaatc aaaaaaacgt tggtggaaca tgtgaagatt ataagaatgc attagtttct
    12901 ttgaaacatc ataaaatatt gagagaaaat atttcaaata tagttagaat gaaagcatat
    12961 gaacttgaac ctaatatgag aaagaagtta gcccttgtga ccattagtat aatgtttaat
    13021 tttaaaatgt ataaaacagc gtataatatg ctctggagaa tatataatcg ataaagaggt
    13081 aggaaatatg aggaaattga cctttgtgat aggaattatg ggaaaaggag gggcagaacg
    13141 agtaattgcg aatttggcaa ataattttat tcaagataac tgggaaattg atattataac
    13201 tatatatggg aatcggcaag actatgaact gaatagaaaa atacaggtac atccaataat
    13261 atgtaaaagt tcgtttcgtg catttagacc tttggaacgc tttaaggaac ttaggaaaaa
    13321 aataagggaa atacagcctg atctggttat ttcgttcctt gctgatgtaa atatccatgt
    13381 cattatttca ttgttaggag taaaacaaaa attaattgtt tctgaacgaa atgatccaca
    13441 acggaatcca gaagaaccat ggattaggaa aattagagat tttctttata aaaatgtaga
    13501 tggaattgtt tttcaaacac cagacgcaaa aaaatatttt agtaagatca atgataaaaa
    13561 agtaaaaaca attataattc cca
//
LOCUS       DACCWK010000063        13537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCWK010000063 DACCWK010000000
VERSION     DACCWK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.063"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            78..3356
                     /locus_tag="IVW65_001829"
     CDS             78..3356
                     /locus_tag="IVW65_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7900703.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3897..5804)
                     /locus_tag="IVW65_001830"
     CDS             complement(3897..5804)
                     /locus_tag="IVW65_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7900704.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5934..6809
                     /locus_tag="IVW65_001831"
     CDS             5934..6809
                     /locus_tag="IVW65_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7900705.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6889..7863
                     /locus_tag="IVW65_001832"
     CDS             6889..7863
                     /locus_tag="IVW65_001832"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7900706.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8052..9188
                     /locus_tag="IVW65_001833"
     CDS             8052..9188
                     /locus_tag="IVW65_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7900707.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9325..9669
                     /locus_tag="IVW65_001834"
     CDS             9325..9669
                     /locus_tag="IVW65_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7900708.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9940..11226
                     /gene="efmA"
                     /locus_tag="IVW65_001835"
     CDS             9940..11226
                     /gene="efmA"
                     /locus_tag="IVW65_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7900709.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11364..11963)
                     /locus_tag="IVW65_001836"
     CDS             complement(11364..11963)
                     /locus_tag="IVW65_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7900710.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12367..13488
                     /gene="mnmA"
                     /locus_tag="IVW65_001837"
     CDS             12367..13488
                     /gene="mnmA"
                     /locus_tag="IVW65_001837"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7900711.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
BASE COUNT         4312 a         2451 c         2892 g         3882 t
ORIGIN      
        1 gttttttatt gacagtaaaa ataaattagg taatacttta agtaaacgct ttctaatttg
       61 ttacgggagg aatacacttg ttaaaaaaac gtataaaata cagtgcgcta atgatgaatc
      121 tccttatgct cagtaccccg gttttaattt ctgtcgttga agttgctgct aacgaacaac
      181 agtctgtgaa cgaagaaaat gcacacgtga acattgaagg gatgtcaaca atggaggaaa
      241 taccaagaac tagtgaagat atggatacgg aagaaggaaa tgatcctatg atgaaagaat
      301 cagaaaatga aaagacaaaa aagcaatctg aagaagcaaa tgaagagtgg aaaggggttg
      361 ttttcggaga aagcacaagt gctagtaggt atgccattga accattagaa gacggcgtgc
      421 ggctctcttc cacgaataac ggcggaaaat tccagtcaag cggatcagat ggaatgacct
      481 attattatca agcaatcccc aaagacatca actttaggat gcgggcgacg atcgaaatag
      541 aatcttggac ctatacgaat gcccaagaag gatttgcttt aatggtaaga gacgcagtac
      601 caaaaaatca tttgtttggg caggcatttt attcaaattc atttgccata ctaggcagtc
      661 ggatcgaata tgtctgggac tcggatagac aagaagtggt caacacgagc ggcagcaaat
      721 atacgatgcg acttggattg gggacacggg caatcacagg aatatcatct gaagatccgc
      781 aagctgcgcc agctcctggg agtgtctcag tggaaacaac acctttagag acttcagcag
      841 ggttcttgga aaaagaaaca ggttcttata atatattcgg aaacggtcat gggaatctgt
      901 ttccggccac gaactcgatt actaaactgg acgtagagat gaaaaaaacg aatactggtt
      961 tagaggcata ttattacgat cctgaaacag gagaagaaat ggcttctgac atcatgtatg
     1021 actgggaaaa attatacgct tcagatgaat cggttattta tgcaggattt gctgcagcaa
     1081 ggaatatgac gatcaaggta gaaggaatcg aagttgctta ctcagatcct gcgacagatc
     1141 ctcctgggca agaaagacct actcgcttga ttgatccgat ttacactgtg acctctagta
     1201 atcattcagg aaaccaagat catactatgt cctttagagc aaatgctgac ggattattga
     1261 caattaaaaa taaagcaaca ggagaggtgc ttaagaacgc aaaaaacttg gctatcactg
     1321 cgaatcggga atttgattat cagacaaaac tgatggaagg aacgaacgaa tttactttca
     1381 gttttacgcc agataaaaac tatccgccag aagaatacgt agagatgact agttatgaaa
     1441 caaaagagat tcttcataca gtggactacc gtaaacctaa atattcgaca gtttatgtaa
     1501 caccagaagg gtcagatgca ggaaacggaa gcagacaaaa tccattatct ctgacacaag
     1561 cattgagata cgcatatgct gggcagacaa tcgtcatggc gcctggaact tattccttcg
     1621 aaagaggatt gacgattcca aaaggcgtta acgggacaag agaaaacccg attcagttga
     1681 ttgcagagaa aaatccagaa aacaagcgtc ctgtacttga ttttaaagga actggaaatg
     1741 ggatgacact gttcagtcac tactggggtt tacgagggtt tgacattaca aatagtgcag
     1801 ctatgcaaaa aggattacag atttctggaa atcataattt gatcgaagag attcatgctt
     1861 atcggaatgg aaatacagga atccaaatca gcggtagctc aaatgatcct ttcgaagcat
     1921 ggcccgcgca taacttagtc aagaactgta catcttttga aaatgccgat ccaggatttg
     1981 aagatgccga tggatttgcg gccaaactga caataggaga aggaaatgta tttgacggtt
     2041 gtatcgcgta tcataatgca gatgacggtt gggacctttt tgctaaaaat gaaacaggtt
     2101 cgatcggaaa agtaatcatc aaaaactcag ttgcttatcg aaacggctgg gtaccaggaa
     2161 ttgagggaga aggtaacggt aatggattca aaatgggcgg ttcttcttta acgggccctc
     2221 atgaattgat caatagtttg tctttcgaaa atcttgcgaa aggaatcgat tccaatagcg
     2281 gaacagatat tatcgtcaat agcagtacaa gttttaataa cggttcatat aatgtggctc
     2341 tttatacctc ttcagcagga aacacagact accatgcttc tggtatctta tctttccgaa
     2401 cagagaatct tgagatgaga gagcagatcc gcccattgaa tcaaaacgaa gatcaaatct
     2461 accactcgtt gaactactat tggaatgaac gagagaaaca atcagaaaat actcttggac
     2521 ataccatatc aaaagattgg atcgagtctc taaatacatg ttcgaaagaa ggtcagcagc
     2581 ctgttacgcg ctatgaaaat ggaagtatcc atgttcatgg actgatgcag attaaaccgg
     2641 ggaatcgtca aacagaaaag gaaagagtgg atggtcttcc attatctgta ggtgctattt
     2701 ttgacggaga aacctcaccg tcttcggaat atccagagta tgcaatcgta caagaaccaa
     2761 atcagacaaa accaaatgaa caaaataatc cgattattat acaagaagca aatggtgtgg
     2821 cggagaatgc agaaggaaat atcattgaac tgaagccttt tacagttttt gtgtcaactt
     2881 tcacaccaaa tcaaggaacg cctgtctttg aagatatgcg ttatttccca gaaaagatcc
     2941 aaatcgttga aacacagtca agtgctcaat tttctaatca taccgctatt ttttcagcac
     3001 cttcaaaagg agaatatacg ctagaagtca cttatcgttc ggaaatattt gatggggaaa
     3061 aatggtctgc tcatacagaa tctgttaccc agcaaaaagc gatcactgtg cgagaaactg
     3121 ccgatgtgcc cccgggaaat gacaaaccag aaagagaaaa tccaggaaat gaatcaccaa
     3181 atggaaaacc aaatggaaaa ccttctcatc cggataggac agcaaaagaa agtgaaaaaa
     3241 atggcactca aggactccca aaaacgggag aatttaataa cccgttgcta gcattagctg
     3301 gaggaatttt gttaatcggt gtagttgttt atgtcatgaa acagcgaaaa aaataagtgg
     3361 aatttctcct tttatgatgt aaaacaattg actgaggaaa gtaaaaatag aaggaaatga
     3421 gaccaataga tgtctcactt ccttctatct ttttatagat gaggttctcc ttgctgcttt
     3481 tgattggctg ttcttattac ttgatgcagg acagcttttt cacaatctct ccactaaacg
     3541 gtgttcaatc agctgaccct cggtattagc tcaacaaacg gcaaaatatg aggaactatt
     3601 tttccgtttt tttctctaat actcaggtca ggacgctgat ttcacaacct ctcgataaac
     3661 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaagcca
     3721 ttttcacaaa atttttctta ctacttgaaa ctaagcagct ttttcacaac ctctaataca
     3781 tggtattcca gaagtaactt cttcgaaaaa taagccgaaa tgtcacaaaa atttgagaag
     3841 cgattttccc aaattccttc ttatttcttg aagctgacca cttctggcac aacctcttac
     3901 cagattcgca ctcttttttc tggcgctaga tacatagcgt cttgttccgt gataccaaat
     3961 gtttcataga attcgtcaag gttttttggt tgcacgtttg ctcggagttt agcaggggca
     4021 tggacatcga tttgcagtaa aagttgttgg tattcttttt ttgcttttgt acgccaaatc
     4081 gttgcccaat tgacaaagaa ttcctcgata gaaagatctg attccgtttt tgctgcttcc
     4141 aatgcacagc ttagaccgcc tgcatcagca atattttccg aaacggttaa ttttccgttt
     4201 acttttccgt cagcaaaagg cagaccatcg aattcttcga tcattgcttg tgctttgttt
     4261 tgaaattgtg taagatcttc ttctgtccac cagttgttca agttgccata ttcgtcaaat
     4321 aaagcgccgt tattatcaaa tgcatgggaa atctcatgag cgataacagc tccgattcca
     4381 ccataattag cagagcttga ttgttctaga ctgtaaaatg gtgcttgaag gatcgctgcc
     4441 ggaaagacga tcacattccg aaatggatga taataagcat tgaccgtgtc agcactcatt
     4501 tcccattctg aacgatccac tggtttgttc catttgctga aacgatcttt taatgtgagt
     4561 ttgctgaaaa caagagtatt tgaaagcaaa gtaccaccct gatctgcagg tactgttttg
     4621 aactggtcga ataatgctgg gatcttatcg gggtagccta cttggatacc taagttattt
     4681 aattttatga cggctttttc ttttgttgca tccccaagcc agtcattttc ttctagacgt
     4741 tttttataaa cggcgatcat ttttttcacc atttgttcta cgtctttttt agcagcttcg
     4801 ccaaaatatt tttttccgta gtaatcgcca actacttgat cgaattgtcc agaggcaagg
     4861 taatacgctg cttttttctg aggcattgct tcgtctgttc cagacaatac gcgagaatag
     4921 attccgctga cttggcggaa ttcttctgat agataaccac taagagaacg tactgtttta
     4981 atgatcatcc agcttttgaa taacggaaaa tgagcttctg tcaggatgtc attcaaatga
     5041 tcgaagtaca ctggatcagt tacgatcact ttatctggag ttgtcccaat caaacccgtg
     5101 acaagttttt tcaagtccag tttatctgtg tatttaataa atgtatcaaa agattgcgga
     5161 ttatacattt tgctgtaatc tgcgttttct tctgcacttt ttacatgagg agcaatcaat
     5221 ttatcaaatt gcactgcttg ttccgtgatt gcttctgcct gacttttttc tttgccggca
     5281 agctctaata actgaacagc catatcaaag aaaacgccta ttagttgtgc accgttggga
     5341 tggtctgatg cataatacgt cttatctgga aggaataaag aaggcggata agcaaagaag
     5401 gcgttcgttt gcgcattctt catatctgca tctacatcca gattaaatgg caatggtaat
     5461 gtatccaaca gccattctgg aaattgctta ttcaattcgt cgaaagactg caatgattca
     5521 actttttcga ttataggttg aagcggttga gcatgcaact catttcttcg gtcataatcg
     5581 tttgccagtt gataatatgc aaggaaatgt ttcatttttt ctgaagacac tttttctgga
     5641 tgggcaagca gatcgtctgt ttctcccatc aaaagttggt cgatcccgtc aactaaatct
     5701 tggaaaccgc ctgtagctgg tttatctgca gggatttttg ctgttttttg ccattcttca
     5761 ttgactgctt catagaaatc ttgtttcaat cgttctttgt tcattcatcg atcgctcctt
     5821 ttttgtttcg tgtgcccatt atcttacaaa atgaaggaat taacaaatag aacataaaaa
     5881 attgaaaatt cgaacaatca aactgatttg tttttttcaa tccttcactt ataatgaaaa
     5941 aaaggaggcg atcccaactg aatcaagtag tggataaacc tttatatttc aaagtagata
     6001 aaaaaattaa aaaattagcc agcactcagc agctgcagtc aagaaaaagt aaattgcttt
     6061 ttttagctct ggtttttgaa gatcaaagtt atgtgattat cgatcaatca ggacatccgg
     6121 tagaatattc tccagcaaaa tatacttatc aagagggtct ttcttgtaaa aaatggaagc
     6181 tgatcaatga agagccaatc gagttatcta gatggatcaa cagaaaagaa gatattcccg
     6241 ttttgatcga agaaaaaagg tcaggaaagg aactcgcaaa ttgctgggtg ggattgccag
     6301 aagaacgttt tttgaggtat aagaaatggg ctaccccctc aggctatctt tgtggtactt
     6361 acgcggcagc agttctcctt gcctattatc aagactatcg taaggggtgg atgcttccaa
     6421 atgagattcg caagaaaaat acggctgatt cgttggtttt gacgaaggca ttgcgtagcc
     6481 agatccaacc actaggactt cctacgattc cttttcaggt tagtacaggg atttctagtt
     6541 ttttaaagaa gaatggcaat catgagcgtg cacgagcaac tcttctaggt tcatggcagc
     6601 gagctacaaa acgtatacgt gaaggcaaac ctgtgatgat tggtattttg aaagtacttg
     6661 gcagtacata tggcaatcat tgggtgacag cgtacgctta ttttgaaact gaaacaggag
     6721 aacgttatta caaagtacac gacaactggg gcgattatca taaagtcatt cctgccagtt
     6781 ggagtaacgg aacagtttca cttccttaag aatttgctga aattaacaga aaatcttgga
     6841 aaaatatgtt aaactatcga agaatagaga agttttgggg aggactacat gagtaataag
     6901 tatcaggatg acacgttgaa gatttttagt ttgaatggca accgtccatt agctgaaaaa
     6961 atcgcgaaag tgttcggaac agaattaggt aaaagcgtcg tgaagcaatt tagtgatggc
     7021 gagatttcca tcaatatcga agaaagtatt cgaggggatc atgtgtatat cgttcaatca
     7081 acgaatgcac ctgtcaatga ttactatatg gaactactga tcatgatcga tgcaatgaag
     7141 cgagcaagtg cgaaaacgat caatgtcgta ttgccttact atgggtatgc taggcaagac
     7201 cgcacggcga aacctcatga accgatcact gcgaaattga tcgctaactt gatcgaagaa
     7261 gcaggggcaa ctcgagtact gactttagat ctgcacacgg ttcaagtaca aggattcttt
     7321 gatattcctg tagataattt atttacgatg ccgttgtttg ctcattatta ccgtcaatta
     7381 ggtctgacgg gagatgacat cgtagttgtt tcgccaaaaa acagcggtgt tcagcgtgca
     7441 cgtagtcttt cagaatattt gaacagcaca ttagccatcg tagatcatgc agatgatgct
     7501 tcaactgagg gtggttatgt gatcggtgat gttcaaggaa agacatgtat catggtagat
     7561 gatatcttga acacaggagc aacgcttgca cgagcagcaa atgtattaaa agaaaacggc
     7621 gcaaaagaag tatatgcctg tgcgtcacat ggattattgt ccgatcatgc gaaaagtatt
     7681 ttagacaatg caccaatcaa agatatttgt atcactgatt ctgttttcac agaagaaaat
     7741 cgtcatccag aaaacttaac aatcgttaca tgttcagaat taatgggtga agcggtcaaa
     7801 cgcatccatg aaaatacacc gatgagtccg ttattccgtc tggaagaaaa agaattcgaa
     7861 taaacaaaaa aaagagggaa agaagttgtg acagaagtga gcagcttcaa ggagctgcga
     7921 caagactttt gtcacagctc cttttttcta tgcccatgaa acgtcctcta gacaaaaatg
     7981 agctcttctt tggtttgttc gctattttat gctagaatgt gcctagcaat aatttggaag
     8041 ggatgaatga agtgaaaact atttatctgg atcatgcggc aactaccccc atgcatccac
     8101 aagtaattga agcgatgacg gcaatcatgc aagacacgtt tggaaatcct tcaagcatac
     8161 atggatttgg tcgccacgcg catgaaaaat tggaaacagc aagacaaatc attgcagaca
     8221 gtctgcaagc aaaacctcac gagatcatct ttaacagtgg gggaacagaa ggggataata
     8281 cagctatctt agaaacagcg ttctcgcgtc aaaaagaagg cagacatcta atcactacag
     8341 ctatcgaaca tcctgctgtt cttaatacca tgaaatattt agagacaaag gggtttgacg
     8401 ttacatatct gcctgtagat gaaaaaggga atctgtcgat cgaagaggtc aaaaaagcgt
     8461 tacgagaaga tacgatcttg gtctctgtga tgtacgggaa caatgaaatc ggaaatttga
     8521 tgccaatcaa agaaatcggt gaacttttag cggatcatcc agcttatttc catacagatg
     8581 cggtacaagc gtacggaaac caaacgatcc atccagaaga attaggaatc gatttattaa
     8641 gtatctctgc tcataaaatc aatgggccaa aaggcgtcgg tttcttatat aaacgagacg
     8701 gagtaaatat ccctgcattg cttttaggcg gggagcagga agaaaagcgg cgtgcaggta
     8761 cagaaaattt agcagggatc tggggtatgg caaaagcagt tgaactgctg tcacctgaag
     8821 aacgtgaagc acgtaacaac aaatataaag gattccagca attgattctt gatacattag
     8881 aagcaaacga aatcccttat caagtaaatg gtgatttaac gaataaactc cctcatgtgt
     8941 tgaatcttcg cttaattggc gaagcaaatg atttgctact gatgaagctt gatcttaatg
     9001 gggtcgctat ttctactggt tcagcttgta cagcaggaaa tatcgagcct tcacacgtat
     9061 tagaagcaat gtatggagaa gatacaccga tactaaaaga atccatccgt atcagctttg
     9121 gtttgggaaa taccgaagac gacatccagt atttccttga tcaattagtt cgtgctgtca
     9181 aaaaataact agtgaagtca ttcttatatg aacagatgtc tgagagaacg ccttcatttt
     9241 ttagggaact taactgaaaa acctagtaaa aatcaggata aacttttata ataaagagaa
     9301 actaatttta tcgaggtgaa aatcatggca tttgaaacaa cagcaacggt actgggcgca
     9361 ccaattagct atcgtttgca tcctgatgca aaaagatata cattgcgcga taatggattt
     9421 acagaaacaa acggaggcaa ttatcaattg atccgaccat tagatgccac gccgcaaagc
     9481 aaagaaggat tcaaattaaa aatcactgta tcaaaagata ttcaaacatt gaaaatgtcg
     9541 atcactacag caaatggatt gaaagcagtc aatattttca aagatccaaa acagaaaatg
     9601 agccaagata aattttattt tctgatggat gggatgatta gccgtgggtt attcgaaaaa
     9661 gtcgaatgat ctgtggatat aaaaagacaa ctagttttga aacggcctat tgttagatga
     9721 ctaactttgt aaggacgacg caaattagtt gtctttttta tttttaaatt taacacatgt
     9781 tgttattttt aatattttgc tgatttattg tttctaagta aagaaattta aattgaaacg
     9841 attgttattt agggaaaaag gtattttttg aaaaaagaat cccgtataat gattgttgtg
     9901 caaacgctat acaaaatgaa aaagaaaaga gggatgttta tggaaaatga acagtcggtc
     9961 gttttaacga attggaagag aaattatttg ttctttttat cgggacagtt tttgtcagga
    10021 atcactagta tggtcgtcca atatgcgatc atctggtatt tgacaagaga aaccggttcg
    10081 gcgacaattt tgagttttgc tactttacta ggaatgattc ccatggtttt actaagccca
    10141 tttgtaggac cgctagtgga tcgctgggat aaaaaagctc ttttgattgt cacggatatt
    10201 attgtagcga ttttcgcgct tattttagca gtagtcggaa cgatttcaga atccttcccg
    10261 atttggctag tttttgtgtc tttgtttatg cgttcggtcg cacaaacatt ccaaatgcca
    10321 acgatccagt cgattatgcc gacaatcgtt ccatcttctc acataacaag gacgaatgga
    10381 cagttaggaa tggtccaatc agcgaatttc atcattgcgc cagctctagg tgcggctttg
    10441 ttttcagtcg ttcctgtcaa ttaccttatt ttattagatg tattgggggc agtttttggt
    10501 gtcggcttat tgatttttgt gaagatccca aaagtttctc ctgaaatatt ggaagttccg
    10561 cttactattt ttaaagatgc aaaatttggg ctgcagcaat tgatggataa taaagggtta
    10621 tggtacataa cgatcaatgg tgcatttgtg atgctgttgt tcatgccagc aatcagtctg
    10681 tatccattga tgacgctaga ttactttggt ggttccgttg gacaggcggg agcagtggaa
    10741 gtagtctacg cagtcggaat gctcttaggc ggtgcactga tcagttttat aggaacatgg
    10801 aaagaccgaa tgaaacctat tattatagcc tacatcatta tgggcctgac gatcggtgca
    10861 agcggattgg ttccaaacga tagtcaaggg ttcttgtact ttcttatctt gaatgcagga
    10921 gcaggttgtg caacgccata tttcaataca ctgctgatgg caatgatcca gcagagttat
    10981 gaatcgaatg ttttaggtcg tgtcttaggg aacttcaact ctttgatgaa cctggcaggt
    11041 ccaattggat tattatttgc aggaccatta gctgatcgtt taggggtaga aaaaatgttc
    11101 ttgttttccg gaatcgggat tttattatgc ggaatcgttt tatttttaac gtcagctgcc
    11161 cgaaattatg ataaagaatt acagaaaaaa ctggtaaaag aacaccatga gcaaaaagac
    11221 gaatagtatg gaatggagtt gtgacagaag tcttgttaca actccatttc tgatcctaat
    11281 gttcctgtgg caataagcct actcaaatga aaaatatgag taattatctt tatttgttta
    11341 atacttgata tagggcagtt ttttcacgac ctatcgtact tcctctttgc ttcatagatt
    11401 tctttgattt gttgctcaga taacggagtg ccaaatggta gtttttcctt taagagttct
    11461 ctttccggtg cgtctacgcc tacattgaca tcgttctcga taggtacgct gtaatgatgg
    11521 atatgtccat gaagattgcg gatagtgtcg ttaggcccga gcaacatcgg ataatgagtc
    11581 aagtaatatt gttgatggtc gaatttaacg atggcaccta catcataaaa ataaaatttg
    11641 ggacttccgt cagtaagcct aggatcattt ttttctaaat aacggaaaaa agcgcgactg
    11701 tcatggttcc ctttgatcaa gtggattgtt ccttgcagtt gagtgaccag ttccaaaatt
    11761 tctgcatttg ctttttcata ttgcggatgc atagcaaggt cgcctagatg ataaacatgg
    11821 tcggtttctt ttattactgc attccagcta gtgatcaact gttggttcat ttcatccaca
    11881 tttgcaaaga gccgcggagc aaagtcgttt tttcctaata agcgttcatg gaaaaagtgc
    11941 atatcactga taaagtaatt catgtatctt ttgcctccct tttttcttag agtaaacaaa
    12001 atcacttatc tgagcaactc ataacatcta aaaagaaaag gttgtaaatg agacgtgcag
    12061 ctttaagcga taagaagcaa ttttgaaaaa tagtttttca caatttataa aattattcct
    12121 tattgccgaa aggctggccc tttttatgag tatgccaatt ttttatatcg aatgctcaaa
    12181 agatgagtga ttttttggtt gactatcatg tttgtgtact gaataggtcc agtagaacag
    12241 tatttttacg acttgataat taagggatga aattttcaga aatatgacgt ataatgtaaa
    12301 atactgaaaa gttgcgacct tcaactcgta gatgtttcag aatctaattg aaatgatggt
    12361 gatatcatga cagacaacag caaaacacgc gttgtcgtag ggatgagcgg cggtgtcgac
    12421 tcatctgtaa cagcactttt gttaaaggaa caaggatacg acgtgatcgg aatattcatg
    12481 aaaaattggg atgacacgga cgaaaacggt gtatgcacgg ccacggaaga ttataaggat
    12541 gtagcgaaag tagctgctca aatcgggatt ccttattact ctgtgaattt tgaaaaagaa
    12601 tattgggatc gtgtatttga atatttccta gccgaatacc gcgcaggacg tacacctaac
    12661 ccagacgtga tgtgcaacaa agaaatcaaa ttcaaagcat tcttggatta tgcaatggat
    12721 cttggcgccg attatgtagc tactggacat tatgcgcaag tcactcgcga tgaaaacggc
    12781 gtggtccaca tgcttcgtgg agtagacaat gataaagatc aaacttactt ccttagccaa
    12841 ttatcacaag aacagctttc aaaaacgatg ttccctctag gcggcatgga aaaatcagaa
    12901 gtacgggcaa ttgctgaacg cgcaggcctt gcgactgcta agaaaaaaga ttcaacggga
    12961 atctgcttta ttggtgaaaa gaacttcaaa caatttttga gcaactatct gccagcaaaa
    13021 aaaggaaata tggtcactct tgatggtgaa gtcaaaggac aacatgctgg cttgatgtat
    13081 tatacgattg gtcaacgtca aggactaggg atcggtggcg gaggagattc gcaagaaccc
    13141 tggtttgttg taggaaaaga tctagcgaca aacacattat atgtggggca agggttccat
    13201 catcctgcat tgtatgccac aagcttggat gcaagtgaaa tccactttac gacgaatgaa
    13261 ccaatgccaa aagaattcaa gtgtacagcg aaattccgtt atcgtcagca agacgttcct
    13321 gtgacagttc gcttattgga tgataatcgt gcagaagtcg tattcgacga acctgtacgt
    13381 gcaatcactc ctggacaagc agttgtattt tatgatggaa tggaatgtct gggtggtggt
    13441 ttgatcgacc acgcatatca agaaacaaaa gttttacaat acgtttaagc gatattaaat
    13501 gaatcaaaac agctatggct aacaaaagaa acacctt
//
LOCUS       DACCWK010000064        13314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCWK010000064 DACCWK010000000
VERSION     DACCWK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.064"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            54..1793
                     /gene="yfcC"
                     /locus_tag="IVW65_001838"
     CDS             54..1793
                     /gene="yfcC"
                     /locus_tag="IVW65_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7900712.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1950..3134
                     /gene="iadA"
                     /locus_tag="IVW65_001839"
     CDS             1950..3134
                     /gene="iadA"
                     /locus_tag="IVW65_001839"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7900713.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3245..3448
                     /locus_tag="IVW65_001840"
     CDS             3245..3448
                     /locus_tag="IVW65_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7900714.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3549..4211)
                     /locus_tag="IVW65_001841"
     CDS             complement(3549..4211)
                     /locus_tag="IVW65_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900715.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4224..5726)
                     /locus_tag="IVW65_001842"
     CDS             complement(4224..5726)
                     /locus_tag="IVW65_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7900716.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6274..7953
                     /locus_tag="IVW65_001843"
     CDS             6274..7953
                     /locus_tag="IVW65_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7900717.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8080..8559
                     /locus_tag="IVW65_001844"
     CDS             8080..8559
                     /locus_tag="IVW65_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7900718.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8778..9458
                     /locus_tag="IVW65_001845"
     CDS             8778..9458
                     /locus_tag="IVW65_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7900719.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9466..10797
                     /locus_tag="IVW65_001846"
     CDS             9466..10797
                     /locus_tag="IVW65_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7900720.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10812..11774
                     /locus_tag="IVW65_001847"
     CDS             10812..11774
                     /locus_tag="IVW65_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7900721.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11885..12223
                     /locus_tag="IVW65_001848"
     CDS             11885..12223
                     /locus_tag="IVW65_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7900722.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12302..13009
                     /locus_tag="IVW65_001849"
     CDS             12302..13009
                     /locus_tag="IVW65_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7900723.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13174..>13314)
                     /locus_tag="IVW65_001850"
     CDS             complement(13174..>13314)
                     /locus_tag="IVW65_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7900724.1"
                     /translation="IYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIK
                     VR"
BASE COUNT         4217 a         2349 c         2917 g         3831 t
ORIGIN      
        1 ttatccattt tttattcatc aaaattactg aaaaaagaaa ggaagaaata aatttgaaga
       61 aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca ccccatacgt
      121 atgtcattat ttttggtgta gtcatctttg catggatact gacttttgtc gttccagcag
      181 gaaagttcag cacgcaagat attgaataca aagatgcaaa tggcgaaaca agtactcgta
      241 ctgttttacg tcaagattcc ttccgttatg cttacgagct ggataagtcg tatgtgtttg
      301 atcaattaga agaactgcaa gatcatccag cagaacgtga aaaactggat gttccggaaa
      361 aaggattgga aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa aaactagatg
      421 aaatctcctt gacagatgat gtgctttacg atcagtacgg cgaaaatatc tatgatactt
      481 ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga tgattttggc ggctttggtt
      541 tcttaaattt tgtttttgaa ggactagtat ctggagataa gtatggatct gctgtaggga
      601 tcgctgcatt gatccttgtt gttggaggtg cttttggtat tattatgcgt accggagcga
      661 tcgacgcagg tatctatgcg tttatcagta aaactaaggg actggagcgt ttagctttac
      721 cgctattatt ctttgctttt tcattcggtg gcgctacatt cgggatggca gaagaggtaa
      781 ttccattctc aatggtgatg gttccatttg tcattgcgtt aggatatgac tcgatcgtag
      841 cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc gacctcttgg atgagcccgt
      901 ttagcgtagc ggtggctcaa gggattgctg gaatccccgt gttgtctggt gcgacattcc
      961 gattgattat gtgggtagtt gttactgcat tagccgctgg ttatatgatg atatatgcag
     1021 aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa atcagatgat tatttccgtt
     1081 ctcatatcaa aaagacatct gatgaaaata aaaaattcat gcttgggcat aaattgatct
     1141 tattagagat gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa aaaggctatt
     1201 acattccaga aatcgcttct caattctttg tgatgggatt agttgctgga atcattgctg
     1261 ttcttttcaa attagatgga atgaaagtca atgacatcgc ttcttctttc caatctggtg
     1321 cagctgattt agctggtaca gcaatcgtag taggtatggc caaagggatt cttcttgtct
     1381 tgggaggatc tgatgcatct gttccttcag ctttgaatac gattttgcat gggatcggga
     1441 cggcgttgac cggtgttcca gctgtcattg gtgcttgggc gatgtacatc ttccaaagtt
     1501 tgttcaactt ggttgttaca tcaaattcag gacaagcagc tttgactatg ccaatcatgg
     1561 ctccactggc agatttagta ggtgtatcca gacaagtagc agttcttgct tatcagcttg
     1621 gttcaggttt tatggacgct tttacacccg tatcagccag cttgattggt gtgctaggtg
     1681 ttgcacggat cgaatgggca aaatgggcaa gattccaaat caagatgcaa ggattcctgt
     1741 ttgttttagg aactattttc atcatgattg ccattgcaat aggtctgcag tagtactgga
     1801 gacctgtaaa taaaaaagta atccaaacag tagataagga ctccaagaac cgtcatttta
     1861 cggtaaaatc aaaggagtaa agatcctatc gtttggatta ttttttaaga tcctatcatc
     1921 agttatactt aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt gaagtgtttg
     1981 caccagatta cttaggaaaa atggatgtac tgattgccgg tgggaagatt ttggctgtag
     2041 aagaacagct agaaggcggc tacgaaggag tggaagtaga agaactttca ggtgagggaa
     2101 aagtactaac cccgggtttc attgactgtc atttccatat tttaggtgga ggaggagaag
     2161 gcggttatca aaatcgaacg ccagaagtca cattgagcca attgactaca gctggtgtaa
     2221 cgacagttgt cggttgtttg ggaacagacg gagaaggtag agatatgact gcgttgatca
     2281 gcaaagcgaa aggtctggaa gcagaaggaa tctccactta tgtatacgaa ggttcgtacc
     2341 gattgccggt aaaaacagta acagattcta tcatcaaaga ctttttgaca attgataaaa
     2401 ttatcggaat cggagaaatc gcagtatccg atcaccgttc ttctcagcca agttttgaag
     2461 agttcgctcg tgcagtagca gatgctcgtg taggaggcat gttatccgga aaagctggga
     2521 tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga gctattgaca cgaatcgtag
     2581 aagaaacaga aattccgatc acacaatttt tgcctactca tgcaaatcgc acaccagaat
     2641 tatttgaagc ttgcgtggca tttgccaaac gtggtggcac gattgacttc actgctagcg
     2701 aagatcctga tttttgggag aagacagatg gtgaagttcg cttcagcaaa gcactgaaac
     2761 gattgatcga ggaagatgtt tctttagata atttcacgat gacctcagat ggacaaggaa
     2821 gcttgcctta ttttgatgaa aacaatcatt tcctagggtt aggagtagga agtgccaagg
     2881 cattgctagt agggatcaaa gaagctgtgc aaaaagaaag tatccctttg gaaatcgcct
     2941 tacgagctat aaccagtaat ccagcacgta tattgaaatt ggataaaaaa ggaaaaatcg
     3001 aaattggtgc tgatgcagat ctgtgtatct tggataaaga aacgcttgat attgatacag
     3061 ttatcgctaa aggagaaata atggtccaag aaaaagaagt caaagtatgg gggactttcg
     3121 aaaaaagttt ttaatcaaca gctctaacta tcttttttag aaaacgagcg ttctgtttgt
     3181 ctgtatctgt tgttgatgtt accctgaagg tagatcaata aacaaactaa gaggaggaag
     3241 cacaatggca gatttaaaag gacgttctaa agatatgaaa gataaagtag tcggtgaagg
     3301 aaaagaagca tacggcaaag caacagacga caagagcaaa gaaacagaag ggaaagcaca
     3361 atcactaggt gctgacatca aagaaaaagc tcgaaattta ggcgacgaag tcaaagacaa
     3421 gttttctcga aaagacgaaa aaaaataaaa accacttatt actaattgaa tcaagcaaat
     3481 aaaaaatccg aaccatatct gcgagacaga tatggttcgg attttttatt tgatcattat
     3541 cctgtttttt aaacttcgat tttttgaaat tttttatttt ttagttcaaa gacttcatca
     3601 cagctttttg ctacttcaat ctcatgagtc acgatgataa tacagcgatt ttgttcatgg
     3661 gcgatttctt ggaaaagttt gacgatatct tgtgaagttg tttcatctag atttccagta
     3721 ggctcatccg ctacgattag ttgatgttca cagcagatag cacgaacgat agcgactcgc
     3781 tgctgctgac ctcccgaaag tttggtcacc tgtttttttg ctaatatttc atcaattcct
     3841 acacgagcca gtcgatctaa agcaacttgt cgataattct cttgcttggt cttagtgatt
     3901 tccattgctg ctatgacgtt gtctagagcc gataaataag gaagcaagtt gtaagcttgg
     3961 aaaataatcg agacatcatt tttacgataa cttcttaacc cgattttgtt cagtgaacga
     4021 tcttgatagt atacctttcc ttcttttggt gcatctagtc cagaaataag cgaaagaaaa
     4081 gtcgtttttc cagaaccact tgctcctaaa atagcgtaca tttttccagc ttcgaaattc
     4141 agttctacat ctttataaag cgcatcttgc ggatcttgat accaaaatcc caattgttct
     4201 gtccgtaaca tcaaattcct cctttagttt aataggattt ttcttgggtt taatcttaat
     4261 atccctatag aagctaaaaa gacagaaaac agactgattc caaatgctac tccggctaat
     4321 agtcccaatt gtgcaggttt gacattgatt tccaattcgc tgacttgatc agaaacagca
     4381 aatggattat tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc ttgtctgtta
     4441 cctggcattt gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc tgtctgttgg
     4501 tcaagtagct gctgtcccac ggcattcgcg acgacgtttc cgctaagcga agcaactcct
     4561 aatgcaagaa tcatacaaat agccacttcg gtaaacaatt ggagaatgat tttacttctg
     4621 gattctccta aagatagcaa gacgcctaac tcatgccgac gctcccgaat ggtaatcata
     4681 atgatcagag taagtatgat aataccggct acagtcacta gtaaaacaac atttttagaa
     4741 aaacttgcca cgttgtttaa aggttccaac attgactgat acatggaatc atttgattgt
     4801 aagctaaatg tatctgtgtc gatcagcttc tcagcttctt tgagaaactc atccatttta
     4861 tctggatcag atagagtgta aatagcagaa tctatcgtgt tatcgtcaga tgtcccattt
     4921 agttcatttg caaaagtgta agaagtgtaa agtgtattcg acggattcat gaagttgaat
     4981 ttcataccca tactgccgct tgtttcgctg gattcataaa ttcctttgat cgtcatttca
     5041 taagtggtat cttcatcttc agtactcgtg attgtgaaag tatctccgac tgccaggtca
     5101 tttgtttctg ctagagtaga atcaatgact acattgtttg tgcctttgtc gctgtcatct
     5161 attccttcac catccgtgat ttttgctgtt ccatcagaaa aattactatt ttgtgcagtt
     5221 gctgacacac cactgatttg aaagtctgct tgagtcattt gcggattccc gcccattcct
     5281 ccagcaggtt caccgcctgc atcagtagtt tctgaactgt cttcacttga atcagaacta
     5341 gtgatggcag ttattccatc ttttgccata gctgaggcgg aactttcaaa ggaataactc
     5401 tttacattat ctaattcagc tattttttcc gcgtccgtta aggaaactgg tgtaagagaa
     5461 aaactgcctg gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt ttgttcctgt
     5521 tttttaaatg aagcttctcg attggcactt aaagtcactg ttgctccaac ttctttttgt
     5581 gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag cacaaaaata
     5641 agaatggctg tgaacacggc aaacaataac aatgaacgtc cccattttac ttttgtactt
     5701 ttccatgcac gtttcataaa attcatattt tgcatccctc ctagtagatg catttgataa
     5761 tagacaacct atcttaaagc tagcttaaat cttatgctgt ttgcacacca tcaacatgag
     5821 gttctgtata tataaaaagt cactatagtt attgcaaaaa atgtggtaaa atgaaaaaag
     5881 tatgtttcaa accgacgaag aagaaatgaa agaggaatag caaaagaaat cctagatgct
     5941 acagagggat ttttacgtgc atagttgttc taacaattgc tgatactagc gatttagttt
     6001 atttgttgta taataatggg gaacagctga atacttatat aagtattctg gctatagcgt
     6061 ttcgcaaaag atccattttg ttaatgaagt atatccaaaa taaaatgccc atccaactgt
     6121 cagaacgcgg atgtgctggt aattttcctg ctgattctcc ataagttgag aaatagtgat
     6181 tttgcatatg cttcattgga atggctcata tatttagata aagaagaaaa tcgacatctt
     6241 taaacttgga tgtcataaga agaggtgaag tttgtgagta ccattaaaat tatcccatta
     6301 ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga ggatattttt
     6361 attttagatt gtgggctaaa atatcctgaa aatgagttgt tggggatcga cgtcgtaatc
     6421 cctgatttta cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt tttaacacat
     6481 ggacatgcag atgcgatcgg ggcacttcct tacttgttgt caaaaatttc tgtaccagta
     6541 ttcgggacag agttgacgat cgagttagca aaattgaatg tcagtcgtaa tgaagcagcc
     6601 aaaggattta aggatttcca tgtggtagat gagcatacag aaatagactt tggtgaaaca
     6661 gtagtgagct ttttccgtac cactcatacg ataccagatt cagtggggat caatctgaaa
     6721 acaaaagaag gtagtatcgt ttatacggga gattttaaat ttgaccaaac ggctatccca
     6781 atgtatcaaa cagattatgc acgattggcg gaaatcggca aagaaggagt cttagcctta
     6841 ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag cttctgaagc ccaaatagca
     6901 gatgaagtat ttgatacgat caaatattgg gaagggcgta tcatcgtcgc ttgtgtagca
     6961 agtaatttgc aacgagtgca acaagtattg gatgcagcag aaaaagcaca acgaaaagtt
     7021 gtcttgactg gtcaagattt cgaacgaatt attcgcacag cgatgcgttt agaaaaactt
     7081 caattaccaa gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta tgatccagaa
     7141 gaactgatca ttttagaaac tggccgaatg ggtgaaccca tcaagtctct gcaaaaaatg
     7201 gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag atttggttta tatcactact
     7261 acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat ttatcgagct
     7321 ggtggaacag tgaagttgat ctctgagaat atgcgcgttt ctggccatgc caatcctaat
     7381 gacttgcaat taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt tcaaggagag
     7441 tatcgtgaat tagctgctca tgcagatttg gctcatgcag tagggatgcc atacaaaaat
     7501 atctatatta ctggacgcgg agacgtgctg gaatataaga acaagcgtat gagtgttgca
     7561 ggaactacaa gtgcagaaaa tgtaatgatt gatggattag gcataggtga tatcggaaac
     7621 attgttttac gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc ggttgtcact
     7681 atcagtcggc gagaaaagaa aatcatttca aaacctcaaa ttacttcacg gggattcgtt
     7741 tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca atatgattga aacaatcgtt
     7801 gagaaacatt tagaaagcaa tgattttgag tggagcaaat taaagcaaga catccgagat
     7861 caattaggcc gttacttatt tgaacaaaca aaacgccgcc cagtgatctt gccagtcatt
     7921 atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg aacctctcta
     7981 tagagtgctc gtcttgctct ttttttgcga aaaacaaata atttttgaat acagcatccc
     8041 tcgctttata ataaacgata gaataaaggg agtgaatcaa tgatgagtca aacaaaagtc
     8101 gaacgttatt tagaagaaca aaagatcagc tatcaaccac ttgattttag tcatgtggta
     8161 gaagaatctt tcacagaaga actacaaaaa agagggattg atccagcttt gatatgtaaa
     8221 actctcgtag tgaaaggaaa caaaactggg gtgatcatcg cattgattcc tttgaacgat
     8281 cacttagatt acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg ttttcctgga
     8341 atggattttg tattaatgca tacaggatat ccccacggag caaacacccc tattggaata
     8401 aaactcgcac atcctgatta ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa
     8461 gtaattgtgt caagcggtga aatcgggaga agccttcgga taaaagtagc cgatttagaa
     8521 aagatcgtcc atcctattta cgctgattta attagttaag ggacgagaag agaagctaag
     8581 cagagcagag acctcaaact tgagattata tctgcttggt acttggtgca tcctgtatta
     8641 tctgttacaa ttgctttgta catactgtac tatcaatcca atgaaaatat atttagcagc
     8701 tatatttttt cattgatgtg caagtgatga aatcgttttt aataaaaaag atacagagaa
     8761 aacgcgaggt gtaaaatatg aaaattacgt atcatggaca ttcctgttta acaattgaaa
     8821 tggaaaatgg ccaacgcgtg atgattgacc cgtttattac aggaaatcca ttgacagact
     8881 taactgcaga agaagtaaag acagattggt tgctgattac tcatggtcat agtgaccaca
     8941 taggagatat gattcctatt gcaaaagaaa atcaagccac aattatcagt attgttgaga
     9001 ttgctgatta tgcgaacagc agaggagtag attcattcgg tatgaatatc ggtggaaaac
     9061 atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca gcatagttca agctatgaag
     9121 tagacggtat cgttcagtat atgggcgagc ctagcggaat tattattcaa gcagaaggaa
     9181 aaacgattta tcatgcaggg gatactgctt atttcagtga tctaggattg cttgcagaag
     9241 aattcgatat tgatgtcgct ttcttgccga ttggcgataa ctatacgatg gggccagaag
     9301 atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc cattacaata
     9361 ctttccctgt cattgaacaa gatcccgaat cttttgtaga gttactggaa gatagtgaag
     9421 gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat
     9481 gatttgattt tagaatatat tgaaagttta cctgttggca atcgaatttc tgtaagaagc
     9541 atcgcaaaag aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa
     9601 aatgtcggac tagtttcaac aattcaacga gtagggacca tccgtatcga acgcaaatta
     9661 aaaaaacata ttgaacgttt gacatttggc gaggtcgtca ggatcattga aggggacgtt
     9721 ttaggcgggg cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact
     9781 aaacatgcca tggaacgcta tattacgcca ggatcactaa tgatcgtcgg caaccgggcg
     9841 gaagtccaaa aattagcatt ggaagatggc gccgcagtat tgatcacagg aggattcaat
     9901 acgactgctg agattgcaaa gctagctgat gatcttgaaa tgccgatctt acgcacctct
     9961 tatgatacat ttacagtcgc aaccatgatc aaccgagcgt taagcgacca attgatcaaa
    10021 aaagatatca tgcttgtcag tgatatctat atgccagctg aaaagactca ttatttgcag
    10081 cagacagata cgatttctga ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt
    10141 ccagtcgtca accgccatca tcgtttgatg gggattgtta cggcaaaaga tgttttaggg
    10201 aaatcgccaa accagatcat cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa
    10261 acaatgagta ttgcctctgt tagccatcaa atgatctggg atgggctaga agtcatgccg
    10321 gtcgtttccg atgacttgac attggaaggc ttgattaccc ggcaagatgt aatgaaagcg
    10381 atgcagcttg tccagcgaca accgcagatt gcagatacga tttcggatca gatatctgga
    10441 aatatccata cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa
    10501 ttcgtagtag caccacaaat ggtcaatggc gttggaacca tttcttttgg cgtacttagc
    10561 gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg aaatatcttg
    10621 attgaacaag tgaacctaca ttatttgcgg ctgatccaat tggaaagcga attagacatt
    10681 cgtccacgtg tattggaaat cggcagacgt tccgctaaac tggatatcga agtgtttatt
    10741 gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa
    10801 gcaggagtga aatgacagta cagcaagaaa tagtagatac aataaaaaaa tacgataaaa
    10861 tcattatcca caggcatttg cgccctgatc ctgatgcatt aggatcacaa gtaggattag
    10921 ccgaactatt acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg
    10981 aagggcttcg ctttttagca gaaatgcaag aagttgaaga tgtcttctac caaggagcgc
    11041 tagtcatcgt gactgataca gctaattcac ccagaatcag tgatcaaaga tatcagttgg
    11101 cagaaaaact gatcaaaatc gaccatcatc ccaatgatga cccttatgga gatcttgtgt
    11161 gggtaaatac aaaagcaagt agctgtagtg agatgattgc agaatttgct ttgatgtttc
    11221 ctgaatcttt aaagatgaac gttaaggctg cacggttact ttatgcagga atcgtcggag
    11281 atacagggcg ctttttgtat ccctccacga cgtcccgtac attagagatt gcttcgatat
    11341 tgagaaatta tccatttgat gcaagtgctt tgaacagaga gattgaacaa atgccgatga
    11401 aagtcgctaa actttccggt tatctttatc aaaatatcca ggtggatgaa aatggtgcag
    11461 gaagaataat ccttccgcaa gaaattcttg ataaatatgg aatcgatgat tcagagacag
    11521 cttcgatcgt ttcacttcct ggagtgattg acgaagtgct ggcttggggg atttttgttc
    11581 agcagccttc agggtattac cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat
    11641 tggctaaacg ccatcatgga ggaggccatc cattagcaag tggtgcgaat gcaaaagatt
    11701 tagcagaagt agaggaaatc tataaagaga tccaagatat atgccaatcg tataagagca
    11761 gaaaaacaga atgatacttc gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta
    11821 tcttttcaag atcttctttt tccgttacaa tagtgctcac taaagatttt aaaggagaaa
    11881 gaagatgaca ttaccaaatt gcccagaatg tggctcagag tacacatatg aagatcgagg
    11941 cttgttgatt tgcccagaat gcgggaacga atggaacaaa gaagaacaag aaactgtcat
    12001 agaagacgga ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt
    12061 gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa
    12121 agtcaagaac atccgtttag tagaaggcga tcataatatc gattgcaagg tagaaggatt
    12181 tggaccgatg aaactgaaat cggaatttgt gaaaaagaat taaagaatat tcaaggtctt
    12241 ttcacattct tttcctacta ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa
    12301 aatgtatggt tatggaatgt atggtttttt agatccgaca tttcttttgg tcattcttgg
    12361 attagttatt tccggaattg cgtcagcgta cgtcaatagt actttccgaa aatacgatca
    12421 agtgagaagc aaaaacaatg tgactggtac acaagcagca caatatatat tacaaagtca
    12481 acagataaat aatgtcggtg ttcaacaaat tgccggagat ctcacagata actacaattc
    12541 aggtaataag atgttgagtt tgtctcaagc aactgcacag tctacttcag ttgctgcgat
    12601 aggtgtagca gctcatgaat gcggacatgc ggtacaagat gcttcgggtt atgtcccatt
    12661 gaagattcgt gcagcacttg ttccagcagc taactttggt tcgatgattt cgttcccctt
    12721 gattatggta ggtgtattgc ttagctggaa ctctacgtta atcaatatcg ggattttcgc
    12781 tttttcttta gcattacttt tccaattggt gacattacca gtcgaattta acgcgtctcg
    12841 gcgagcaatc caaattttga gtgaaggcgg actactgacg gaagaagaag taccgatggc
    12901 aagacacgta ttatttgcag cagcactgac ttatgttgca gcagcgcttt cgacattcct
    12961 gcagctgttg cgcttgatct tattgttcgg cggcaaccga agagattaaa ataaagaagt
    13021 tgtgaaatca gcgctttgat ctgagctaat accgagagtt agctgattga ataccattta
    13081 tttggaataa gggctctttg tcaataagga ctgatgggtt gtacaaaatc aaatctgggt
    13141 cagaatgaac ctcattctgg ctcagatttt ttgttatctt actttgatta attgattgat
    13201 caaaaagatt ctaattctca tgttctcaaa tgatttaaat ccgtaggata ctcgtttcat
    13261 tgtctttata tgggtattct tcgcttctat ttttccattg gtgtaaggat agat
//
LOCUS       DACCWK010000065        13006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCWK010000065 DACCWK010000000
VERSION     DACCWK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.065"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(222..1670)
                     /gene="cls"
                     /locus_tag="IVW65_001851"
     CDS             complement(222..1670)
                     /gene="cls"
                     /locus_tag="IVW65_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7900725.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1822..2715)
                     /locus_tag="IVW65_001852"
     CDS             complement(1822..2715)
                     /locus_tag="IVW65_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7900726.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2699..3385)
                     /gene="ftsE"
                     /locus_tag="IVW65_001853"
     CDS             complement(2699..3385)
                     /gene="ftsE"
                     /locus_tag="IVW65_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7900727.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3490..4591)
                     /gene="prfB"
                     /locus_tag="IVW65_001854"
     CDS             complement(join(3490..4527,4529..4591))
                     /gene="prfB"
                     /locus_tag="IVW65_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7900728.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4717..7251)
                     /gene="secA"
                     /locus_tag="IVW65_001855"
     CDS             complement(4717..7251)
                     /gene="secA"
                     /locus_tag="IVW65_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7900729.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7473..8024)
                     /gene="raiA"
                     /locus_tag="IVW65_001856"
     CDS             complement(7473..8024)
                     /gene="raiA"
                     /locus_tag="IVW65_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7900730.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8152..8871)
                     /locus_tag="IVW65_001857"
     CDS             complement(8152..8871)
                     /locus_tag="IVW65_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7900731.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(8868..10181)
                     /locus_tag="IVW65_001858"
     CDS             complement(8868..10181)
                     /locus_tag="IVW65_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7900732.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(10262..10582)
                     /locus_tag="IVW65_001859"
     CDS             complement(10262..10582)
                     /locus_tag="IVW65_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7900733.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(10690..10977)
                     /locus_tag="IVW65_001860"
     CDS             complement(10690..10977)
                     /locus_tag="IVW65_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900734.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            11104..11748
                     /locus_tag="IVW65_001861"
     CDS             11104..11748
                     /locus_tag="IVW65_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7900735.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(11807..12514)
                     /locus_tag="IVW65_001862"
     CDS             complement(11807..12514)
                     /locus_tag="IVW65_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7900736.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         3598 a         2818 c         2256 g         4334 t
ORIGIN      
        1 ctcattaaaa gtttctatgc tatactggct ttgaaatgtg tctcttttaa tagcatatat
       61 tagcttattt cttgctttcc ccaaaaaaca agagataccc caaagttaat ttcgctcatg
      121 cgagacgctt tctccatttc tgacgtactc tcagaaatgg ccccgttaga aaaggacacg
      181 agatctccat ctgttgatgg ttctcatgtc ctttttatgt tttaaaggag cggcgaaatc
      241 aatcttgcta atccttcttt catcttgatg attattcctc ggttttcata catttctttt
      301 gtgagatgcg tagaaatttt cgagtcttca aaaaaagctt ttcttacacg tgaagccatt
      361 cgttcatcat aaaccagtgt attgacctca aagtccaagc gaaatgaacg aacatcgata
      421 tttgctgagc caactgaaac aatgccacca tcgataatca tcgtctttgc atgaataaat
      481 ccattctcat atgtttcgat ccttgctcca tactcgatca gctctgcagc gaaagaatac
      541 gttgcccagt aaacgagaag atgatctggt ttgttaggta tttggatatg aacttttacg
      601 cctgaaagaa gagccaactt cagtgcttca tgaatcgatc catccggtat gtaataaggc
      661 gtttggatca atatctcccg tttggctaac gaaatcattt tcaaataagt cattttgatt
      721 tgttcatgtt cggtatctgg tccgcttgtc acgatttgaa cagctatatc accagtagtg
      781 tgaatggacg gaaaaaattt gggagaatag cctacttctt ttgtatgctg agaattccag
      841 tccatcaaaa aacggttttg caaggtatac accgcttctc cgtaaatacg caaatgattg
      901 tctcgccaat aaccaaattt ctttaccaat cctagatatt catttccaac attgaaccca
      961 cctgtatatc cgattttccc atcaataact acgattttac gatgattgcg ataattgatc
     1021 cgaggattaa tatacggaac aaatagagga aagaaaaatg cgacttctcc gcctgctttt
     1081 ttcaaattct gaaaaaagct ggaagaaact tgtgtggaac cccatgcatc aagaaggact
     1141 ctgactttta cccctcgtct tgccgcctct gtcaatgcat ctcgaacttc ttcacctaat
     1201 gcatcactgc gataaatata atactggaag tggatatggt cttctgcctg cttgatatct
     1261 tcaatcagcg catcgaattt ttcacgacca tctgtaaata aagtgatttc atttttagtc
     1321 gtatagagcg tactttcaaa taccgtgagc atatagatca attgtttcac atctacttgt
     1381 ccagtaggcg gatgaggata aagccctcgc atcaatgcct gtttgtcttc ttcaatttcc
     1441 aggttcttac cgattttccc ttggatcttc aaatcaaaga ttttctcttt ggaaatccct
     1501 cttcctaaaa aaatatataa aataaatcct acaactggaa tgaacatcaa aacgagcagc
     1561 catgcccatg tttgagcagt ttcctttctt tctcgaaaaa cgatgataaa ggaaagcaaa
     1621 atattcataa tcaaaaggat cgtaaaaaaa ttgtcaacga taattttcac ggcttcacct
     1681 cttcttctac caacttaaga atacccaagt tgttttttaa agtaaaggga aaaaagccgc
     1741 ttccttctat tctcctagcc aataaaaaaa gaaagctgga acgaaactgt ccaactttct
     1801 cattttattt atattgtggt attagaattt caagaaacgt ctcatagaaa tgatcgagcc
     1861 aagtgaacca atgatcacac caatgccgac cattaaaaga ttgattttcc aaacgatatt
     1921 ttgtggatga atcaatgaat aatgggaacg taataatgat ggattgatca attggaaaaa
     1981 ccatgcataa ccaaatgtga tgatcaaaat cggaagaatc gcaccgatca gaccgatcca
     2041 gccaccttca aggaaaaacg gccagcgaat atatccattt tttgcaccga ctaaacgcat
     2101 gatttgaatt tcacgctgtc tagacaaaat cgtgatccga atcgtgttcg aaatgagaaa
     2161 catcgctacg aacagcagca atgccgctgc aactaatccc catgtccgaa ctgcaccagc
     2221 gatttggaaa atccgatctg aggaagctcc accgtaatca gaacgagaaa cattcttcaa
     2281 atctgctgct tcttctgcta cttgttttgt ataagaagga tcagtagcac ttactacata
     2341 cacatcatat aacgggttat tgtcaccttc aaacaatttc caggaatcac ccatttcgtt
     2401 ttggattttt ttcaattcct gttctttgct tgaaaattca acttttttaa catgatctaa
     2461 gtcattcaat tctttttcta gctttttcat ctcttcgtct tttgtaccga tatctacgaa
     2521 tacagataca tcaacgtttc cttctatatc ctgagctaat ttagttgcat tcatgataac
     2581 tgccatgaat acaccgacta aagtcaacgt gatcgttacc gcactgactg aagcaactgt
     2641 catccagccg ttacgtttca aactcttgat actttctaat aaatggcgga aaaaggttct
     2701 aatcatcgta tccatattcc ccttccgctt gatctcgaat aatgcggccg ttttcgatag
     2761 caattactcg gtgccgaatc gtatttacga tcgtactgtt atgtgtagcc atcacgacag
     2821 tagttccttg ggcattgatc cgatctaata atttcatgat ttcccatgag ttttctggat
     2881 ccagatttcc tgtcggttca tccgcgataa gtactttagg cgtattcacg attgcccgag
     2941 caatagagac acgttgctgt tccccaccgg ataattcgct aggaaagact cgaactttgt
     3001 gtttcaaacc aacaaggtcc agcacttcca ttactcgttt tttgatatct cgaggttttc
     3061 tgccgataac ttgcatagcg tacgcaacat tttcatatac tgtttttctt ggtaataatt
     3121 tatagtcttg gaacaccacg ccgatttctc tgcgtagata aggaacttcg cggtttttga
     3181 ttgctattag atcatgacct gcgacgttca aagttccttt tgaagctttt tcttctcgat
     3241 acatcaattt gatgaatgta gatttaccgg ctccagacgg accaaccaca tagacaaact
     3301 ctccttgttc gatgacaatc gagagattgc ggatagcggt cgtaccattt gagtacttct
     3361 tcattacatc cttcatttca atcatggcgt tctctccata tctttttcag tctttagcta
     3421 ttatagcatg gcaatgtttc aacatgcttg caaaatctta caattttatt tcaatttaac
     3481 aatgagttct tattgtagat gcatttttaa atagccgtca ataaataaat caaggtcacc
     3541 gtccataact gcttggacat ttcctgtctc tacgtttgtc cgatggtctt ttaccataga
     3601 atagggatgg aaaacataag aacggatctg tgaaccccag ccgatttctt tttgttctcc
     3661 tcgaagtgca gctgcttctt gttctttctt ttccacttct aattgataaa gcttagcttt
     3721 taacatcccc atcgcttgct cacggttttt caattgtgag cgttgtgcct gactagctac
     3781 tactgttcct gtaggcaagt gagtgatccg tacagcagat tctgttttat tgatgtgctg
     3841 accacctgca ccacttgctc ggtacgtatc gattttcaaa tcatctggat tgatttctat
     3901 atcgatcgtt tcatcaagct ctggcatcac atctaccgaa caaaaagaag tatgacgtcg
     3961 tttcgctgag tcaaaaggcg aaatccgtac aaggcgatga acaccttttt ctgattttag
     4021 ataaccaaaa gcgttatgtc ctttaatcag cagggtcaca ctcttgatcc ctgcttcatc
     4081 acctgcttga taatctagtg tttctacttg atagccatgc tgctctgccc aacgtgtata
     4141 catacgtaaa agcatgctgc cccagtcttg ggactccgtt ccgccagccc ctggatgcaa
     4201 ttcgacgatc gcattgtttc gatcataagg tccgtctaac agcatagata attcatacgt
     4261 cttcattttt tcatctaatg cattgatacg tgtttctgct tcttcttgca ttgctgcatc
     4321 aggttcttct tgcagcatct ccaccatcaa ttcgatttct tctagttctt cggcaagaga
     4381 atggaattgg tcataggttt ctttattagc gttattttcg ctgatcactt tttgtgcggc
     4441 ttcgctgtca tcccaaaaac caggttcagc catttttgct tctgcttccg cgatgtcttc
     4501 ttccagctga tctaagtcaa agagacctcc tgaaacttgt gaccttttcc tgcatttctg
     4561 tcaggagatt tcgaatttcg gatatttcca tgttttttcc ttctttcact atactttttt
     4621 cattatatct agtctaacag aatcagcaaa taacagaaag aaggtgagac gaattcttaa
     4681 aaaaagaatt tgtctcacca atcctgctgt cagaaattag tttgcatttt ttccatgaca
     4741 gttcttatat tttttcccgc ttccacaagg acaaggatca tttcttccga ccttgttcac
     4801 atggattggc tgttttttcg cgctagtatt actattttct ttggcatctc cgtcttctga
     4861 gtgagaagct gttccttgag aaacctgttc acgctgtacg ttttgacgga tctcagcttt
     4921 cataaagatt cgagtgactt catactcgat ggcgcctacc atatcttcaa acatcttata
     4981 gccttctgtt tgatattcga ctaatggatt gttctgtcca taagcacgta agccgattga
     5041 ctgacgtaat tgatccattg catcaatatg gtctgtccat ttagagtcca caacacgcaa
     5101 gatcacgact ttttcaaatt ccaacagttg ctctggacca tttagttgag ccgctttggt
     5161 attaaatact tcttgtgctc gttcgtttag atactctttg atctcctgag atgtttttcc
     5221 ttcgatatct gcaagagtaa tactatcttc atggacaaga gtactgccgg caaaatcgat
     5281 gatactttcc aaattccaat tttctttctc taattgtgta tgcgcatcta ctacacgact
     5341 gattgtccgt ttgaccatgt tcattagtgg cttggataag cttttttctt ccatgattac
     5401 ttcttgtcgc tgtttataga ttacttcacg ttgttcacgc atcacatcat cgtattgtaa
     5461 gacgttttta cgtgtgtcat agttatttcc ttctacacgt ttttgtgcag attctacttg
     5521 tttcgtcagc atcttgcttt ggataacagc atcttcctca ccgatcttca tccgatctaa
     5581 gaaagctttg attcgttctg aaccgaatcg tttcatcaaa tcatcttcca atgacaagta
     5641 gaattgtgac ataccagggt ctccttgacg tcctgcacgt cctcgaagct ggttatcgat
     5701 acgacgagat tcatgtcgtt ctgtcccgat aactgctaga ccaccaagtt ctcttacccc
     5761 aagtcctaac ttgatatctg ttccccgacc agccatgttg gtagcaatcg tcacagcacc
     5821 tttttgacca gcattcatga tgatctctgc ttctttaaag tgatttttcg cattcagcac
     5881 ttcatgagga atacgttctt gattcagcat atctgataat aactcagatg tttcaaccgc
     5941 aacagttccg actaacacag gttgaccttt gcggtagcga tctttgatat cttgcacgac
     6001 agcgtggaac ttgctttgta atgttgggta aagtaaatct ggacgatcat cacggataac
     6061 cggacgattt gtcggaatct ggtatacttg gatgttataa atttctctga attcttcttc
     6121 ttctgtttta gctgtaccag tcattcccgc aagtttcttg tacatacgga aatagttctg
     6181 gaaagtgatc gtcgccatgg ttttcgtttc gtcttcgatc tctacgcctt ctttagcttc
     6241 gattgcttgg tgaagtccat ctgaataacg gcgtccatcc atgatccgac cagtgaactg
     6301 gtcaacaatc agtacttttc cgtcttggac aacataatcg atatctaaaa gcatgatata
     6361 gttggctctc aatgcttgat ccaagtgatg ggtcaatgca gtattttcaa tgtcgtataa
     6421 gttatccaaa ccgaatgttt cttctgcttt ttcgatccct gcttcggtta aactgattgt
     6481 ttttgattgt acatcgattt tataatcttc ttcttctttc aaacgtttca cgaagttgtc
     6541 tgctcttgta tataaagctg tggatttttc tgcttgccct gaaatgatca gcggcgttct
     6601 cgcttcatca atcaagattg agtcaacttc atcgacaatg gcataattca gcggacgttg
     6661 taccatttga tggcggtaaa cgaccatatt gtctctcaag tagtcaaatc ctaattcatt
     6721 attcgtacta taagtgatat cacaattata agcctcgcgt ttttcatccg aagattttga
     6781 gttgatattc aaccccactg ttaatcctaa gaaattataa agttcgccca tttccgtcga
     6841 gtcacgggtt gctaaatatt cattgacggt aactacgtgt accccttcgc cagtcagagc
     6901 atttagatat actggcatcg ttgcggtcaa ggttttacct tcaccagtac gcatttcagg
     6961 gatatttcca tcgtgaagca cgattccacc catcaattgt acgtggtatg gatataaccc
     7021 caatacacgt tttgctgctt ctcggacgac tgcaaaagct tccggaagta attgatctaa
     7081 tgtttctcct ttttggtatc tgcccttgaa ttcagcagtt tttgcctgta attgttcatc
     7141 agttaatgct gccatttggt cagcgtatgc ttcaactttt tttgcaatgc tatctaaacg
     7201 ttttagttct ttcttatcgt tttcgataag tgaacgaatc aaattggcca tgcgttgtgt
     7261 aatctccttt tccttctgtg tattatccag actatgtaga ctttactttt cattttatca
     7321 ttagttatag ataattgaaa tatcttaagc gtattaaagt aagaacttca taatttattc
     7381 atatttcatc taaaagagga ggaaactcaa aaaaacttgc ggcagctgtc agtagctgct
     7441 gcaagttttc gttgttattt aagtaaggct gatcagtctg tttcaatcag tccgtatttt
     7501 ccatctttac ggcggtaaac gatacttgta ccatttgttt ctgcatcttc aaaaataaag
     7561 aagttatgtc ccagcatgtt catttgtaag actgcttctt cactatccat tggtttcaat
     7621 gaaaggcgtt tcgtacgtac gatatctaat tcagaatcat tttcgttttc gtcctcttca
     7681 ttgaataaag cagcagcatc tgcagtattc atgcccgttt cacgggattt acgattaatt
     7741 tttgttttga atttgcggat ttggcgttcc aatttatcaa caaccaaatc gatacttgcg
     7801 tataaatctg gagaagtttc ttcggcacgt aatacaagat aaggtaatgg gatcgttact
     7861 tccacttttg ctgttttttc tgtataaact tttaagttca catgcgccgt tgcatcgggt
     7921 gcgtcactga aatagcgttc tagtttgcct acttttttct caacgtagtc gcgaatcgct
     7981 tcagttactt cgatattttc gccgcgtaca ttatatctaa acataaaaat tacccctttc
     8041 gtctaccaca atcaagaagg agaaggacca ctctttccct cttcttaatt ctattatatc
     8101 gaactctagg ttatttgcaa attaaatcgc tatcaaattt ccacatctaa ttcatctcgc
     8161 caaagaaatg gttcggatga ttttcggctg tgcgacctgc aaacaatctg ctgcgtggaa
     8221 aagagtctgt cctgttgtat agacatcatc aataatcaat atttttttat tttgtatctt
     8281 agaaaaggtg actgcaagtt caaaaggttg aggcatctcc aatctttcct ttcgcgtgtt
     8341 ttttgcttga gccagcagat gcttctctct tttgagtagc atctgaaaag gaattcttgc
     8401 tgctttcaac atctcgctta cttgattgaa tcctctttct gttcgccttt tttcagataa
     8461 aggtattgga caatagatgt atgtcgggta ttcttttttt atctgcgtcc atttatatgc
     8521 aaaggtgcct gctagtcgga tatctcccat aaacttgtat tgattgagcc aggccttcat
     8581 attttcatcg tacacaaaaa aggcacggtg ctgaaaatca taggtcggat atttcttctt
     8641 ccattcttga cagtcactgc agtaaacggg aatagacttt actttctttt tcgtactctc
     8701 tagattgaaa tggattggtt tttgacagcc agaacagcct cttgtttcca ataaatgaaa
     8761 ttgtgcgcga caaactgaac aagtctgagg taatctgtat ttcgaaacac caataatctc
     8821 gctgaatgtt aaattttgga agatatcttt actgcagcaa ctgcacctca cagcatcttc
     8881 ttcccttctt cattcatttc tttgatttcc tgacaagctt ttcgaatagc tgcagttttt
     8941 tcttcataga aaaagtatac ttgactgttc gtataaggcc ctttacgatc ggctcttcct
     9001 gcgatttgta ccaaagaaga tttagtaaaa accggatgat ccgctcctaa aacgataacg
     9061 gatattcttt caaatgtgac tccacgttca aggatcatcg tggttaacag tatatcatat
     9121 tttgcttctc gcattttctg gactttttcc gcccgctgat tgtctcttga agaaacatct
     9181 gtgatctcat atctcggaag agcctcttga agtgtggctt tcaaacgatg catcatcgtg
     9241 atacttggac aaaataaaag gatatggttt tctttaatta attgatgaat gatccgcacc
     9301 aaagatttta tcttcttctt gttcttgcat ttttgtcgcc aattattcat gaaaaccaag
     9361 acgggttcag gaagcaaacg ccgatgaaag cgtaaagcaa gtttgtggat gtcaaacgtc
     9421 tgcttgattt cctttagtaa ctttttatca ggtgtggcac ttaagtagat gaatcgtccg
     9481 tctggtttaa tcgctgtttg cacagcatga gctaatcctg catccccgtt ataaggaaac
     9541 gcatcgatct catcgatcac tattaaatca aaggaccgat aaaaacggta tagctgatga
     9601 atcgtacaga taacaaaaga agtaaaggta taagccgttg ctgtattccc atggagcaac
     9661 gtgatttttt cttctggaaa aacataacga tagcgtaaaa acaattcatt acaaacatcg
     9721 actcgcggtg tacaaatcgc tactcttcct ccagctttta aagtttccac taaaatagga
     9781 taaagcatct ctgttttccc tgcacctgtt acagcccaaa taagatgatg tcttttttct
     9841 gaatggcaaa gtattctctt tgagatttcc gtttgttttt cagtaagctg tcctgtccaa
     9901 aaaaagacga cttctcgagg ttttgcttca ggctgatcaa acaaatataa atcttgttga
     9961 gtgtcgcatc gccctaaata tagacaatgt gtacaaaaat aaccgtaatc tgcaagctgt
    10021 ccatcaatga cggacgtcct cattccacag cgatgacaaa gaatccagtt gcccaaacgt
    10081 tccactgctg cgatcttctc ataatttggt aaatttacct cctcacagtc tattgcttca
    10141 atatctctcg tcagaagcct tctaccccaa aaatgattca tattcttcgc cctaaaaaaa
    10201 tatacgaaaa aacaagcgat ctattttttg gatcgcttgt tcgatagtta tactgttcta
    10261 tttattcacc tatgagcgtg attgcttgat ctaacacttt ttcgccattc atcataccat
    10321 aatcagccat attgatgaca tctaaaggaa tgccttttgg ttccagacgt ttctcaaaat
    10381 cagctttcat aaagcgaact tgtgggccta ataacaacac atccacgtct ttatttgcaa
    10441 ggttgttatc tgcgtcagaa gcggacacag cgaaaatatc cgagtcgatt ccttttgctt
    10501 cagcagcttt ttgcattttt gttactagta aacttgtgct cataccagcc gaacatacaa
    10561 gcatgattgt ttttttagcc atacttttct ctccttttta aaacaaacta tgcaaacgtt
    10621 ttcttcgcgt ctgttactct attatataaa aagaaagaaa tttgtcaacg ttttctaaac
    10681 gcggtttatt taatcaaata gccaactaga tttcactaat ttattgaaca cgacatattc
    10741 tttgtccaaa ttgatgacaa ctacttcata gcctgcattt atccagccat aggcaccttt
    10801 ttcagggtgt ttataatcat ttgcccacca atctgtactt tcccttgctg ttttgggaag
    10861 tccgttactg ggaaacagca gttcgtcaaa ttgggtaaag gtcaacgtta cttgagagcc
    10921 gccattgttc ttcaaataat gcgtaattgc atcgtacttt ttcattcgtt cctgcatccc
    10981 cagatccacc tgcctttgtc tttattatcg catactacag caattgcgca accgttacag
    11041 tgttgaaaca acctagaact ttatttatac tgaataaaga aaatgaaaaa aagaggtgac
    11101 aagatgatcg agcgctatcg tacaataaaa gaaaatggac aaagtgagat tgagatcaaa
    11161 aaatcacgct ttatctgttc tcttagacga atagaatcag aggaccaagc aaaaagtttc
    11221 attcaagaaa taaaaaaaga acattggaaa gcgaaccata actgcagcgc cttcatcatt
    11281 ggagaaaaaa atgaaatcca gcgaagcagc gatgatggag aaccaagtgg aacggcgggc
    11341 gtaccgatgt tggaagtcct caaaaaaaat caactgatca atgtagctgc agtagtcact
    11401 cgatattttg gaggaacgaa actcggcaca ggcggattaa tacgagcata cactcatgcg
    11461 gtctctcaag cattgtctga cattggaatc gtagaaggta ccttgcaaca ggagatctct
    11521 gtgatgatca cgtatccgca actaggaaaa ttacagaatt atcttgaact ccaacatttt
    11581 tttataaaag aaatccaata tactgatcaa gttagcgtca tctgtgctgt agatgaaaaa
    11641 agtgctgacc actttaaaga atccatcatt gagctgctga acggacaagt ccaatttgaa
    11701 gagggttctg tttcttatca cgagcaactg ataactgatc atatataaaa acaggtgatt
    11761 ctgattctct tttatttcta ataaggaatc agggatcacc tgttttttat ttttccaaat
    11821 aaaactcaaa acgatttcct acgtattgcg tacgcacata ctcaaacggc gtaccgtctt
    11881 caaaataaga aatctggcgc aaacgaagaa ctgcatctcc tcgttttacc tctaaatagt
    11941 cagcaatttt ctcagaagcc aacacagcag taatcgtttg attcgctgga ccgatctttt
    12001 tcccgccttt ttcttctagc gtgcgataca acgattcagt aatctctgct ttgctgtagc
    12061 catcgatcag cttttgtgga acactggcaa cttcaaaaca aatagggaca tcatctgcga
    12121 aacgaatacg ttccattcgc aagatcgtgt catctttact caagtttagt ttttccattt
    12181 cacttgagct tggcgaagtc aagaaataag agattgtccg actagacggt actcgatttt
    12241 gtgattccat aatttctgta aaacttgttg caccagacat tttttcttgt actttttctc
    12301 gcgcaacata cgtacccgaa ccgatctttc tttctaagat tccttcatct gccaagtttt
    12361 gaattgcttg tctcaacgtc atacggctga cattgaattt cactgctaat tctctttctg
    12421 aaggaagacg gtctccgact ttccatatac cctgttcgat ttcatttttt aattggtcat
    12481 gaatctgtat atatacaggt aattgattcg acataacgta cacccgtcct ttcgtttctt
    12541 ctattataac tggtatatac ctgattcaca actgaaaaag ataaaggttt ctatccatca
    12601 acagaggatt tagacacaca tcgttctccc tactcctatt gtcagaaaca attttttaga
    12661 tatgttcaac ctagaaaata acaaaagacc ctaaaatcga tgaaaatgcc gacttatgga
    12721 tcttttgtta ttattagcgg tttttagcca tccatttttt tgtatgattc gttgggcgtt
    12781 ttgattttac ctggaccgct tttccgcctt ttttcggaat attcacttca atattgtatt
    12841 tttctgattt gaccaaatca tataactttt cagctgcttc gtctactttt tctttccatt
    12901 tcatgtggat ccctccaatt cttgtagtta acttaactct atcttaattc agcagaatct
    12961 tcaaggaaca aggtagcaaa gcaaaaaaaa gagctctcga actaac
//
LOCUS       DACCWK010000066        12995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCWK010000066 DACCWK010000000
VERSION     DACCWK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.066"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            9..911
                     /locus_tag="IVW65_001863"
     CDS             9..911
                     /locus_tag="IVW65_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7900737.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            complement(951..2831)
                     /locus_tag="IVW65_001864"
     CDS             complement(951..2831)
                     /locus_tag="IVW65_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7900738.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(2927..3778)
                     /locus_tag="IVW65_001865"
     CDS             complement(2927..3778)
                     /locus_tag="IVW65_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7900739.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(4104..5888)
                     /locus_tag="IVW65_001866"
     CDS             complement(4104..5888)
                     /locus_tag="IVW65_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7900740.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(5888..7639)
                     /locus_tag="IVW65_001867"
     CDS             complement(5888..7639)
                     /locus_tag="IVW65_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7900741.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(7780..8004)
                     /locus_tag="IVW65_001868"
     CDS             complement(7780..8004)
                     /locus_tag="IVW65_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7900742.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            8255..9841
                     /locus_tag="IVW65_001869"
     CDS             8255..9841
                     /locus_tag="IVW65_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7900743.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            complement(9907..11682)
                     /locus_tag="IVW65_001870"
     CDS             complement(9907..11682)
                     /locus_tag="IVW65_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7900744.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVEKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGCLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(11895..12797)
                     /locus_tag="IVW65_001871"
     CDS             complement(11895..12797)
                     /locus_tag="IVW65_001871"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7900745.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
BASE COUNT         3806 a         2755 c         2314 g         4120 t
ORIGIN      
        1 gtatctacat gttattacgt gaaaaaatca aagaaatctc cttttctcct tcagaaaatg
       61 aagtgatcca gtttctctta agcgattctc cagcacttgg cgaaatgacc atccaagcga
      121 tagctgagcg atgttatgtc cacccgtcta cactcatccg tgtagcaaaa aaaatgggtt
      181 ttgatggctg ggtcagtttt cgtaatgctt ttttagctga aacagcctat cttgaaggga
      241 attttcaaga cgtagatgcg aatcttcctt tttccaccac agacggtatt atgacaatcg
      301 caaataaaat ggcttcttta gaacagatga cgattcatga tacactttct ctcattcatc
      361 atgaccaact gcaaaaagct aaatatttac tgctccaggc aaaaaaaatc gtgatcttcg
      421 ccagtaactc gaatactttg atttctcaag atttcatgct gaaaatgaag cgcatcaaaa
      481 tggatgtcac aattgtcacc acttttggtg aatcattcta tgaagcgtac aattgtacga
      541 gcgatatgtg cgggattctg atttcttata cgggtgaaaa caatgtgatc cttcgtaccg
      601 cagaaatatt gaaacaaaat gatgttccaa ttttagcttt gaccagtatc ggtgattcaa
      661 cgctatctgc aatgagtaca gccgctttgc gacttacgac acgcgagcga ctgtattcaa
      721 aaattggtaa ttttacgatc aatacatcaa tttgttatct tctagacgtg ttatatagtt
      781 gtgtttttgc agaaaattat caacagaatt tagtccatct aatttctgtc ggtcaaaaat
      841 tcgataaacg ccctatcagc tcagccatca tgaaagagcc tttcaatttt gaaaataaag
      901 aagtggaata aaaagaatag ttaagcctag tccattcttc acctaaccgt tcaggtcagt
      961 gcggtgataa gtgcatcgtc tggcccgacc gtctcccctt ggttttccaa aatatccaaa
     1021 taatccgtgt aattggtgac gatcacaggt gtttctaaac tgtatccttc cttttcgatt
     1081 ttctttttgt cgaagcgtag caacagatcg ccttgtttca ctctttctcc ttgctgcact
     1141 aagctttcaa aatatttccc ttccaactgc acagtatcca atccgacatg aatcagcaat
     1201 tccagccctg tttcagaaac cattccgatt gcatgtttcg tcgggaacag ggtcataact
     1261 gttccgtcaa aaggggcacg tacttctcct ttttcagggt agattaagat tcctcgacct
     1321 agtgttcctt gtgcaaaagc tgcatccttc gctgcattta gtggagcgat ttgtccttgt
     1381 atcggtgttt ttacaatttc tttacctgat tttttctgta tagcatctgt ttcacctgct
     1441 tcatccttcc aaaagaaaaa agtcagcgaa aacccgatta ccgaagcaat agcaatgccg
     1501 ataaacgctt gaaacatacc actggcgtct gtttccgtga tgtagtttac caccccaaaa
     1561 atccctaatc cgcccattat gtagctgatt gttccagatg ccatcaaata gattcctgaa
     1621 acagcaccac caatcatcga gtaaataaat ggcttcttct tcggtaaggt gattccataa
     1681 atagccggtt ctgttacgcc gaagattcct gaaatcacag ctggcatgca aagcgccttc
     1741 attttttgat ccttgaactt gaaatacatc gccaatacca ctgctgtctg tgcaaaactc
     1801 gcgccataaa cgcctactaa catctgactc cacccatttt gcgctagctg cataatcgca
     1861 agagggataa tactccaatg aagtccgaaa atcaccagta cttgccaaag aaaccctaga
     1921 actgcaccat acacagctgg agaaaagtcc atcaatgctt ggaatcccgt gcttaataga
     1981 tctgtcagta tactgacaat cggaccgata atcaaaaagc cagcaggaag tgcgattaat
     2041 aacacagaaa aagaaaccaa aaaggtctgg acgactgcag gaatccattt cttggcaatt
     2101 ttttggactt gtccggcaaa tgcgataatg aagataacag gtacgacgct actagtataa
     2161 ctgctgccca cccatgggat acccaaaaat gtttcataag ccggaagacc cagcaagcta
     2221 taaggcgcag gactgccatc agggaaacca ctttgtagtg ctgctccttg tacagtcgga
     2281 taacaaagtg ctgcaccaat cacgagcccc accatcggat tcagatgaaa ttttttcgct
     2341 gctgtgtacc ctaagatcac tggcatgaaa taaaaaattg catcccctat cccattcagc
     2401 atgatgtacg tacccgatcc ttcagtataa aatttgagaa acaagaacaa agcatttaat
     2461 cctttgacca ttcccgctgc agccaaagca cctaaaaaag gctggaaaca gccacttaat
     2521 atgtcgatta atcgattgaa cagattacta cttgaagaaa cctcttcttc tgcttcatcg
     2581 gaaatcccgg ctactgtcag tacttctgca tagacatctg gcacatgatt cccgattaca
     2641 acttgatatt gcccgccgct tttcatgatg gtgactatcc cttccatctt tttcaaaaca
     2701 tcatcctttg ctttattttc ctcctttagt ttgaaacgaa gtcgcgtcac acagtgtgtc
     2761 aagctacgga tattttcttt tcctccgata ttctttacga tgttttctgc taattcctgg
     2821 tattttccca tttctattcc ctccaattaa ttagtggaag gtttagccaa ctgaatgtca
     2881 ctacccttat ttcatacgtt cttgttaatt tgtcctcctt ttttatttat ttacaacgat
     2941 tctcatgcac taaacgcgca gtatggatcg ttaaatacat tttttcatcc gtggacatct
     3001 ggtagtcgaa agccccggtt aaaaagtctg ctattttctc aacgcaacca aacgtatcct
     3061 tatatttttt ttgaataact tggaacaatt cctcagaaac ttctccatct tctgatttcg
     3121 ctccatctag gacacgtgtc agaaaaaaac gcaggtgtgt gacgaatcga aaaaagtgga
     3181 ctgaatcttc atcaaatgta atcttatagt aatagcggac gatatttaaa atatcctgta
     3241 ttgttgtggt aaacaggtag gtattgtctt gctggctttg aatgccttgt gcattgacta
     3301 aatgcaatgc gataaatccg gcttcgtctt ctgataactt gatgtggtat ttatccgaga
     3361 ttttcttcac tgtttttttc ccaatggcat actctttagg aaacagttgt ttgatatcat
     3421 acagcaatac atttggcaca aagatcccca ttttttgacg ttcgatagct gtatacaagt
     3481 gatccgtcaa tgaaatatac acagaatcat ttaaatggac ttgcagagcc tcttttgtat
     3541 attcaatgat ttcacaagaa atttctaagt actcaagagg aatcgcttgt gccaattctt
     3601 ggaactgttg tgaaagtccg tcatttgtca actgaaattt tttgtcgatg catgagtcaa
     3661 gaatcgggtc acccaccttc tttttgaatg ccaatccttt tcccatcaca atcatttccc
     3721 gatctctttc atcccttgtt attacgacgt tgttattcag tactttctca atcagcatgt
     3781 tttgctcctt tccatgatat atagggctgt atccaaaaca aaaagaaaaa cccaaaaagc
     3841 cgtccatgaa accatgggag actcatcagg tttagcttgc atcaggcaat cactatccaa
     3901 gttgtatcgt tttctttata ttatcgaaag caaacgattt tgtcaacgct ttcttagggg
     3961 ctattaggca cttctgacta gctgacgtcg tcttttttaa atctggtaac tacgacacta
     4021 actctcacag atgaaaaata tgggaagcta tgttttattt tcactctagt gcttaggtca
     4081 aaatgctact gtcacaacat cttttatata ccactacttt gcagtttgta catatctgca
     4141 tacaaaccat tttgcgctag aagtgtttca tgattcccgc gttctactat atgacctttg
     4201 tctaaaacta aaatcaattc tgcatctcga atcgttgaca gccgatgggc aatcgcaatc
     4261 gttgtccgtc cttgtcgcat tttcgccaat ccttcttgga tcaatccttc tgtttcagta
     4321 tcgatatttg ctgttgcttc atccaatact agaattttag gatcagtcac gattgttcgc
     4381 gcaaaagtga tcagctgtcg ttgtccacta gaatagctgg caccatgttc gatcactttt
     4441 gagtgatatc cttttggtaa ttcatcgatg aatttgtttg cttgaacaaa ctcagccgca
     4501 tggcgtatct gttcgtctgt gatagctgga ttcatcatcc gaatatttcc tgcgatatcc
     4561 ccataaaaca agaaagcatc ttgcaagacc agccccattt tcttacgtaa ctcttccatt
     4621 ggatattctc gaatgtcatg atcatcaatc aatatctgac cttcataaaa ttcgtaaaac
     4681 cgcatcaaca cattgatgat cgagcttttc ccacttccgg tgtgtcccac taatgcaacg
     4741 gtttctcccg gattagcaac aaaagagata ttgtttaata cattgtgctc tccatcataa
     4801 gaaaagctta cattgcgaaa ctcaatctta cctgaagtaa tcatttgatc tgctccaggg
     4861 ttttgttgtg gtgcatattc ttccgtatcc ataatcttca agatacgact accagcaacc
     4921 atcccatctg tgaagatgct caaaaagtcc atcatttgag acattggatt aaaaaatccc
     4981 tggacataag tcgtaaatgc atagatcagt ccaacttcta cgggagaatg caacgcatcg
     5041 atcccaaata aagtcaacgc caacgcaatc gccaatgcat atagaaaatt gatgatcgga
     5101 cttaacagaa tcgaattcgt tttgatcatc gcatagcgcg tgtctaaata ctcattgttc
     5161 acttgttcaa attctttagc taaacgagct tcttggcgaa actgctggat aatacgcatg
     5221 ccggaaatcg attcattcaa ttttgtattc agctgactta atttttcccg catatttcgg
     5281 taaatacgag aactaaattt ctggtaatac cagatgacaa ttcctaaaat cggcaagaac
     5341 accaaagtcc acaaggcaat ttctccattg atttgaaaca tggcaatgaa tgaagaaacg
     5401 actgcaaaga tcccagtcaa taccatcaaa aagacgtacc aaaattcaaa tagcgtttct
     5461 gtgtcattcg tcactcgaga aacggttgat cctgccggtg tctgatcaaa ataacgcata
     5521 cctagtgtat gcagcttttc aaataatttt accctaatat actgataggt cttcaatgaa
     5581 gccatggaat acaaatacca ttggaaaaac caaatgatac tttttactag gactccaaat
     5641 aagtacatac cagcaaaaaa caaaataact tgtgtcgtag ccgactgttt agccagatga
     5701 tcatccataa aagtttggat gatccttggc aataggacat tgatcacaga cagagcaaac
     5761 gcaaataaga tagcaacgat gaacgttgtt ctaaaaggtt tggcgaactt catcagtcgt
     5821 ttgatgatct ttttctgttc tttaagtgga atcgattttg tccattccga ttgataattt
     5881 tcatccatca ggcctcgcct ccttctatct tcgcttctaa ttgctgtttc tcccacattt
     5941 gttcatacca gccgcctagt gcaagcagct catgatgggt tcctcgttca atgattttcc
     6001 cttgatcgac aacaatgatt tcttttgcat gcatcacgct acttaaacgg tgagctgcaa
     6061 tgatagttgt tttctctttt cttatttctt ttaaattatg caagatagct tcttctgttt
     6121 tggcatctac tgcagaaagt gcatcatcta atattaataa ttcaggattt ttgatcaatg
     6181 ctcgagcaat cgaaatcctt tgtttctgtc cgccagataa agagactcca cgttctccaa
     6241 ctaaggtatc ataacctttt ggtaattcct tgatttcatt gttgataaat gctaaagaag
     6301 ccgcatgctc tacttcttct tctgaaagtt ttggattaga aaaacggata ttatctcgaa
     6361 tggtcatcga aaacaagaag tgatcctgag gcacatagcc gattgatcct aataaagcat
     6421 tcaatgaata ttctttgatc gaacgtccgc caaattggat tttcccttga taatcatcgt
     6481 attcacgcat cagtaatttg atgatcgttg tttttcccgc accagttttc ccaacaatac
     6541 ctaatgtttc tccttcttgg attgcaaact tgatttgttc taatgctggt gattcttctt
     6601 tcggataatt aaaagaggaa atagctacat tcagctcgcc ttttgccggg gtctgcactg
     6661 cacccagttg ttccacgata tgcgtttttt catgcaaaag ttcattgacg cgatcataag
     6721 aagcatttcc tctttccaat acgttgaaca atcgaccaat cgcaaacatc ggccaaacaa
     6781 gcataccgat ataactaata aatgagacca tctgcccgat cgagatcgtg ccttccaaga
     6841 catactttcc acctaaaata attgttgcaa cataagacag accgataatc aaggtaatga
     6901 atggatcaaa taaggagtct agaaaattga ctcgtttgtt tttaataatt gcatcttcaa
     6961 tcttttcttc aaaatccgct agatcttctt tttcttgtcc aaatgtttta atgactttga
     7021 tcccagtgat actttcttgt gtcttatcat tgatactaga aaaagcttct tgcgaatcac
     7081 gaaaagcatc atggagtttc gaccccagca ctcgtgaagt gacggccagc aaaggcagag
     7141 gaatcagcgc aatcagtgtt aagcgccaat caataaacaa aatcatcgca atgatcgttg
     7201 ttccgcccgt aatcactgaa tctgcaaagg tcaagatacc agctcctgct acattctgga
     7261 tcgcattcaa atcattcgtt gcatgagcca tcaaatcccc agtccgatat ttttggtaaa
     7321 agagattgtc catttttgta aaatgtttga ataactgctg ccgaagttct ttctccaacc
     7381 gagctgcact tccccaaata ttcgtccgcc agatataacg aaaaagatat tgtaagaaag
     7441 cagcgataat caataggaag atccacccga aaatgatctt tactgataaa tttttatcgg
     7501 caatctcatc aatgatgatc ccaatcactt ttggaggaac taactgtacc aaggccactg
     7561 cgaatagcga gaaaacccca attaaataat gtttcttttc ctgacgaaaa aaccagccta
     7621 actttctaaa aatcgacatt ttactcctcc tttgtcttta tcaagagcct aggattgtct
     7681 gtgcttgtct ttttgctgaa taatctgaat agtctaacaa attacaaaat aaaaaacgga
     7741 ctgaaaaaat cagcccgtaa aaagtcgttg ggtcagaacc tattttttcc cttggttctt
     7801 catttgctgc atcatctggc gaattttctt ctcagatggt ttttgtccca ttgacatcat
     7861 cattgatcgc aacatgtcct cattaacggg aggatttttt tctaaatagt ctttcatata
     7921 tttacgcgct aggaaaaagc cacctactgc gccgattagc aaagcaataa tagcaataag
     7981 tacgacaata cctgttgata acatataaat ctctcctttc tatttccgaa ttgctctcaa
     8041 tgacttattt tactaaagtt tattgaaaaa agaaaggcgt attctttagt ttcatgcaaa
     8101 aaatagtggt ttttttagag gagaacaaaa tttctttcgt catctagcaa gacgagccac
     8161 aaaaaaaaat taggcatacc gaatattttt ctttaatttt ttctttttga ggtgtagaat
     8221 gtaatcgtag ataaatagga aaggtggttt tttcttgaat aatcaatcca ataaacaatt
     8281 tcataagcat cgacttattg aacatacaaa tggtttatct ttagctgaag taaatggaac
     8341 agttgatgta ccaaagggga aaagtttttt taaaacactt cttgcttact ctggaccagg
     8401 tgcattagta gcagttggtt atatggatcc aggtaactgg tccacttcga tcactggtgg
     8461 acaaaatttc caatatttat tgatgtcagt tattttgatg tctagtctga ttgctatgct
     8521 tttacagtat atggcagcaa aattaggaat cgtaacccaa atggatttgg cccaagcaat
     8581 ccgagcacgg acaagtaaga cattgggtgt cattttatgg atcttgactg aattagccat
     8641 tatggcaaca gacatcgcag aagtcattgg tgcagcaatc gccctctacc tgctctttga
     8701 tattccatta gtgattgccg tctttattac tgtattagat gttttcgttc tcttactact
     8761 gacaaaagtc gggtttcgta agatcgaagc actggttgtc tgcttaattt tcgtcatttt
     8821 attcgttttt gtctatcaag ttggcttgtc taacccagat tggcacagtt tatttgctgg
     8881 ctttatccct aacagccata cattctccga ctcacctgtc attggtggcg aaactccgct
     8941 gactggagct ttagggatta ttggtgcaac agtcatgccg cataacttat atcttcattc
     9001 agcagtttca caaactcgga agatcgatca ccaagacgaa gaagatgtag cacaagctgt
     9061 acgtttttct acttgggact ctaatatcca attgtcgatg gcttttctag tcaatgcgct
     9121 gctattgatt atgggtgtcg ctgtcttcaa aaccggttct gttcaagatc cttccttttt
     9181 tggtctgtat gaagctttat ctgatccatc agctatgagc aatggtatct tgattgctgt
     9241 agcaaaatct ggtatcttgt ccactttatt tgctgtagca cttcttgctt ctggccagaa
     9301 ctctactatt actggtacgt tgaccggtca agtcatcatg gaaggattca tccatatgcg
     9361 tatcccgatt tggctaagaa gactggtcac acgattgctt tctgtgattc ctgtattgat
     9421 ttgtgttttg tttacaagta caaaaggaac gatcgaagaa cataccgctt tgaataactt
     9481 aatgaatgaa tcccaggtgt tcttggcttt tgcgttgccg ttttcgatga tccctttact
     9541 gatgatgacc aacagccaag cggagatggg tagccgattt aaaaattcat tcatcgtaaa
     9601 acttcttggc tggttctctg tgattagtct aacttacttg aacttacgag gattaccagg
     9661 acagatcgaa agtttcttcg gcgagcaagc cacttccgct caactagcat tggctgatca
     9721 catcgcttat gtaatcattg ttcttgtcct tctcctgttg gcatggacga ttggtgattt
     9781 atataaagga aacaaaaaat atgaacagcg tttgcaggaa acggtagaag aaatcgaata
     9841 aaaactgatt tttccacaca aagacccttg atagttaggt taaaaatcta gctaccaagg
     9901 gtctttttat ttagaagata aagctttttt cagatttaat ttactgaaaa ctagcatgct
     9961 tgttactacg atgaattgcg ttaacggcac gcctgcgcca gtgtagacac ttaaaaaata
    10021 atcctttttt tctggcgcat aagaacaaaa ccacgagaca ccgaaaagaa aaaccaattg
    10081 tgtcagtatt aatccggtga ccacaatcaa agaggaacta taataggcac gatcagcttt
    10141 tgcaaatacg gttcctagta tcataagtaa tatccctgct attgaagcaa tcagcacggg
    10201 aatagccaga acttggtaaa tttttatgat gacattcgcg acttttgctg attttgttaa
    10261 ttggaaatta ggtgttctcg gttcattact atcgatccaa ttctccatta gcggaaggtt
    10321 taataattct tgagcctgtt tgacttgttc ttgcgttccg aaactataat ttcctccttg
    10381 atggaaatct ttatatcgcc atgcataagc gtatcctgat tgaaggaaat cgaatacgat
    10441 tggcagatca ctcatcgtct ttccatctcc tgatcccatc agatagaact cttttttctt
    10501 tttgatggtt cccttgtgat aggcttcttc taattcttgg ttgacttttt tccaaaaatc
    10561 attggtttct tttgcattgt tttggtaata accagattcg cttaccacat ctcgcaatgc
    10621 ccaaaagatg atatctccgg gtaattcttt tacttttcca ccttgagtcc acgcgtgagt
    10681 cgtatataat tcgtcaatac gggtagaaac agtatggaat gtaggagatg cggctaatgc
    10741 tttatttagt gcagcatgtg agacccatat ctcactgttt tctttattca agttcgtttc
    10801 atcatctatt cggatcagtt gtttcatcaa acatccaaat tctccactgg aacggtcatt
    10861 gatggttgca ataccgtatg ttgtctcatt tttatattcg atccactgat agacgatgga
    10921 aaagccaact ataggaagta aaaacaatga aagtttcagc cattcccgtt tcgagagaag
    10981 aaagagtcgt ttttttaact tctctacttc tttgaaagaa aagtcttgcc ctagcaagat
    11041 ctggacgaaa ctgataccca aagctactac agtaaaagga agaagccaga tagaatcttc
    11101 gcgaatgaac caaaagaaag gaaaagcgac agaaagccct aaaaaccatg gcagcaattt
    11161 tctgctagaa ccgtatttgc ggtaataaat agctagtaaa caagcgataa tgatctcaac
    11221 agccggaatc acgagggcat tacgatacgt tcgagtggaa tattcatgtg taaaactaat
    11281 cggagaaaac aaaaaataaa aatagaaact taaaagaaga tacttgttct tcacgatcgg
    11341 tttaaacgcg agtgcaacca atatactggc aaaaatactg aacatgccga acaaaactgc
    11401 ataagaaaga tgcagtttat gtgcaagtac taaaaataag ctgtaggaga ttcctttgga
    11461 aagtgttgta gtcgtatact cccctagcca tttaccgtcc aaaatatttt gagcatagcg
    11521 aatcagcagc tggtcatcat aggttgccga catgtctgtt ccatagattg ctttactata
    11581 taaatacaaa cgaataattg taaataatag ggctatgcca agaataaagt atgtatgttt
    11641 cttttttagt tggttgatca agggttttaa agtgttcagc atcttttcgt ccttccaatc
    11701 acgttttgcc acttgcttgt gaaacttttc gtttttttac ttttctacca gtataccctg
    11761 tttgtctaaa tttaaaaaga ggggggagaa gatgcatgat gaaaaagact gcaaaaagca
    11821 ctcctttgtt tcttaaacaa aaaaggacaa ggcaatgtgc ctggtccttt tttgctgctg
    11881 tcagatagaa aatcttagag tatcacttct tctgctaata actcaggaaa cacttctgga
    11941 tcgaatattt gtgctttgaa catggcgatt tcttttccat atggtgcgta agctttcttt
    12001 ttatctttgc cgacataggg agtttccaag attttcgaaa tattgtcaaa cattggatca
    12061 tggacgatct tattcagcgt gtcgaatccg attgttccaa aaccaatatt ggcgtgccgg
    12121 tctttatgcg aaccttgcgg atttttagaa tcattgatat ggagtacttt aagccgatct
    12181 aacccgatga tcttatcaaa ctcttccatt acacctgaaa agtcttcttt aatattataa
    12241 ccggcatcat tcgtatgaca ggtgtcaaaa gtaacggata atttttcatt caattttact
    12301 ccatcgatga tttgcgctaa ttcctcaaat gttcgaccga tttccgtacc tttacctgcc
    12361 attgtctcca aagcgatttg tgccgtctgt tcttttgtca aaacttcatt caagccttca
    12421 ataattctag caattccggc ttctgttcct gcccctacat gagcacctgg gtgaagtgta
    12481 atttgagtag cgcctaaagc ttccgcacga acaatctctt ctctcagaaa ttggacggca
    12541 aaaggaaaca tttctggttt gatcgtattc cccaaattaa tgatataagg ggcatgaacg
    12601 acgatatttg ttagaccgtt ttctgccatg tacgcttttc cggcatcaat attcatctct
    12661 tcgatggctt ttcttctagt gttttgaggg gcgcctgtat agatcataaa agtcgaagat
    12721 ccataactgg ctgcttcttc tgctgctcct aacagcattt tctttccgct catgctgacg
    12781 tgtgagccta ataacatgga tggattcctc cttgaaaggc aattgttact aacaattcct
    12841 atatattggt aagatgatat acaaagaaaa tgattgattt ttctttgccc tgttctccat
    12901 agtaactgaa attttctcta aagaaaagct taagaactct tttataaaaa agatcatttt
    12961 ctcacgtttt aactcctatt atttgtcaaa ttaag
//
LOCUS       DACCWK010000067        12931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCWK010000067 DACCWK010000000
VERSION     DACCWK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.067"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            131..3658
                     /locus_tag="IVW65_001872"
     CDS             131..3658
                     /locus_tag="IVW65_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7900746.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="IVW65_001873"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="IVW65_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7900747.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="IVW65_001874"
     CDS             7446..7787
                     /locus_tag="IVW65_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7900748.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="IVW65_001875"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="IVW65_001875"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7900749.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="IVW65_001876"
     CDS             9144..11564
                     /locus_tag="IVW65_001876"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7900750.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IVW65_001877"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IVW65_001877"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7900751.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4394 a         2247 c         2740 g         3550 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgtg cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca g
//
LOCUS       DACCWK010000068        12770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCWK010000068 DACCWK010000000
VERSION     DACCWK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.068"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..361)
                     /locus_tag="IVW65_001878"
     CDS             complement(26..361)
                     /locus_tag="IVW65_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7900752.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            647..2527
                     /locus_tag="IVW65_001879"
     CDS             647..2527
                     /locus_tag="IVW65_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7900753.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKIGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2606..2833
                     /locus_tag="IVW65_001880"
     CDS             2606..2833
                     /locus_tag="IVW65_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7900754.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2838..3380
                     /locus_tag="IVW65_001881"
     CDS             2838..3380
                     /locus_tag="IVW65_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7900755.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3631..4308
                     /locus_tag="IVW65_001882"
     CDS             3631..4308
                     /locus_tag="IVW65_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7900756.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4629..5870
                     /gene="sstT"
                     /locus_tag="IVW65_001883"
     CDS             4629..5870
                     /gene="sstT"
                     /locus_tag="IVW65_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7900757.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            6017..6652
                     /locus_tag="IVW65_001884"
     CDS             6017..6652
                     /locus_tag="IVW65_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7900758.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6843..7586
                     /gene="truA"
                     /locus_tag="IVW65_001885"
     CDS             6843..7586
                     /gene="truA"
                     /locus_tag="IVW65_001885"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7900759.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7820..8653
                     /gene="msrA"
                     /locus_tag="IVW65_001886"
     CDS             7820..8653
                     /gene="msrA"
                     /locus_tag="IVW65_001886"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7900760.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8734..9727
                     /locus_tag="IVW65_001887"
                     /pseudo
     CDS             8734..9727
                     /locus_tag="IVW65_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            10016..10714
                     /locus_tag="IVW65_001888"
     CDS             10016..10714
                     /locus_tag="IVW65_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7900761.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10864..12180
                     /locus_tag="IVW65_001889"
     CDS             10864..12180
                     /locus_tag="IVW65_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7900762.1"
                     /translation="MRGSGMSFMDKLAKVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISYLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVLNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            12196..12654
                     /locus_tag="IVW65_001890"
                     /pseudo
     CDS             12196..12654
                     /locus_tag="IVW65_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT         4154 a         2070 c         2688 g         3858 t
ORIGIN      
        1 aaatactcgt ttacataaaa aaagattatt tagatgcgat aatatcatag ataaagaaac
       61 attcatcata agaaacagtg atcccaaatt ctgtttcaat atttttgaag gcctttttta
      121 catgaaaaat tgtttcatga gaaggtcttg tgtcatcttc tgatataatt ttaagttcat
      181 gaccgtttat caatgtacgc tcaattagac acgctgtatg aatcaaaagt tttaaaagaa
      241 tggaatgagt gaattttttg tggaaagtac attccaattc ttggataaaa tccattaagc
      301 ttttttggat cttattccca gctaataagt agagatattt gtccactgct tcaactccca
      361 tatcaaaaaa cgatatttct tgtgaatgag tggctgctgc ccttttttga ttgttgttct
      421 tcagtcgtaa gatgacaaag catcttatct ttgaataaaa aaatagaact acctaaggaa
      481 gcgtttcggc gcttcctttt tctatatgat cgtttaatga ctgtaggaat tcagtatacc
      541 aaaagaaaac cgcctttttg aataaacttg atagtggtca attttttatg atggataagt
      601 atctatactt agtctcgtaa gaatgaaaag gaaaagaggg agaaagatga aattacaaaa
      661 gtggatccaa gagcatcgaa atcatatcac cgccttatca gctgtgttga ttatttttgg
      721 ttttatcggg aaatttactg gatttgtctt gttctataac attgctctag ccatagcttc
      781 tattattgcg gtcataccta ttataatcca tgcgtatcag gcagtaaaag tcaaagttat
      841 ctcaattgat ttactcgtta ctattgctgt gcttggtgcc tttatgatcg gagaatacaa
      901 ggagtcagct atcgttacat tcttgttttt gtttggtagt tatctggaac aaaaaacatt
      961 gaccaaaacg cgggaatcaa tcaaggcttt gaccgagatg gccccatcga ctgccagcgt
     1021 tgtccaagaa gatggaacga ccgaagaagt ggaaatcgat gacgtggaag aaggcgatat
     1081 cgtcttagta aaaactggtg gagctattcc tgtggacgga cgagtcattg acgggaaagg
     1141 gtatgcggat gaagccgcag tgacgggtga atcacgacaa gtcgtcaaaa tcagtggaga
     1201 taacgtattt tctggaacga ttttagcaga tggctacgtg acgatcgaag caactaagat
     1261 cggagatgat acgacctttg ccaaaattat agaattagtc gaagaagcac aagataccaa
     1321 atcacatgcg gaaaaattca ttgatcaatt ttctcagtat tatactccaa ccgttttact
     1381 tattgcgttg cttgttggtc tgatcagtcg tgattttcgt ttagccatca ctgttttggt
     1441 tcttggttgt cctggcgctt tagtcattgg tgcgcctgtt tcgaatgtag caggaatcgg
     1501 taatggagcg aaacggggca tcttagtcaa aggcggtgaa gcaatggata ctttttccaa
     1561 aatcgatact tttgtctttg ataaaaccgg tacattgacc aaaggaacta ctgctgtatc
     1621 cactatcaag aactatacag aggatgaagg ggacagctta gctttagtgg ctcgattaga
     1681 aacgttatcc gatcatcctc tggggcgcgc cgtgatccat tatgctcagg agaaagacat
     1741 tgattttcaa caattagcag tcaaagataa tcagacgatc aagggacaag gaatcacagc
     1801 tgagattgat ggtcatacag tttgtgcggg caacaccaaa ttgattgccg ctcacaaact
     1861 ttctctcaca ccaaagcaag tacaagattt acatcaatta caacaaagag gcagctcagt
     1921 cattattgta gcaatcgaca aaaaaatcac tcaaatcatt ggtgtgtcag atgtgattcg
     1981 accagaagtc gctgaacaat tagctatgtt gaaacaagca ggagcaaaac atttagtgat
     2041 gctgacaggt gataatcaga tgactgctga ttatgtagct gacatgttag gcattgatga
     2101 agtccatgct gaattattgc cagatgaaaa ggttcaattt gtgaaaaaat accaagaaca
     2161 aggattgcac gttgctttca ttggagatgg aatcaatgat tctccctctt tggctgcggc
     2221 tgatatcggg attgctatgg gatcaggaac ggatgtcgct atcgaaacga gtgacgttgt
     2281 cttgatgcaa tctagttttg gatcgttggt tcatgcttac cgtttggcta agaaaactgt
     2341 tttgaatacc cgtgaaaatg tagtgattgc cattggtgtc gtcttatttt tactcatcgg
     2401 cttatttgcc gggtttattt acatggctag tgggatgttc gttcacgaag ctagtatctt
     2461 gatcgttatc tttaacgcga tgcgtttatt gtattttgga cacgaaagca aagaagaaaa
     2521 atcgtaaaac ttgataagaa tcaatttttt tatggaagct gtaatctaga ataagactat
     2581 caagaaaaag gaaggaagaa ctacgatgaa aaaaatgatt atgcaattag atacactagc
     2641 ttgtccaagc tgtatgcaaa aaatagaaca tgcggtttct caacagcctg gggtaagcaa
     2701 tacgaaagtt ttgtttaatg cgagcaaaat caaagcaaac tttgatccag atgaagtcca
     2761 ccctgaacaa ttagctgcag ttgtggaaaa attaggttat gaagtccaat caacaaaagt
     2821 aaaagaggcg taaatcagtg aatatagtgg aagaaaaatt tgctcaagaa acaaaacaag
     2881 cagatatcga tcatcataaa ccaactgctg gcgccatggt ggggcatgta ttggcaaatt
     2941 ttaaattaga agaaatgaaa ttgatccaag cacaattgta tgttttgggt acatcttcgc
     3001 aagaattaag tacccttttt tcagaatatg ctgatcaaga agcgagatgg ttcaataaaa
     3061 tctctcatga gttagtgatt gaaaatgaaa tcgttccgtc tactttagaa gaagtcatgc
     3121 gttatggaaa aatttacgaa catggtcaaa acaaatattt accagcctca acaatgttgg
     3181 aaaactttgc aaaagatttt gatttccaaa atgtctttat cacacgggca atcaaattag
     3241 ctgaaaaaga agaaaaatat ggcttgaaac aattgcttgt tgaactttta agtttcaata
     3301 aagaaatcat ctaccaagta caaagactcc aaggaaaaac tgtccgacaa gatttggatg
     3361 aggaagacga tgatgactaa aagtctatgt ttcttcaaat acaaatataa agcgtgaggt
     3421 aaattggaaa atcgcgaaga ttaaaataaa agaagcaccc gctgatgtat tacagacaac
     3481 atcagcgggt gcttcttagt ttttaaaagg tttaaaagat ggatgagaaa atacaaaaaa
     3541 tcaataaaat cacattgctt atttgataga ataaagaagt gggagtaatc gaaaggagtt
     3601 ttttaattta aaaaaaggat tttagtaaca attggtctag cttttttgtt agctggttgt
     3661 accaacggga atgaatcgaa aaaagaatca acaaaagaaa cagcgaaagc aacaattgca
     3721 actactacaa caactagcac agagagcaag ccaactgcag caagtacgga aacagcagat
     3781 acctctactt cgtcagagtc ttccaaaacg actgaaacaa ctcaaacatc tacttcaagt
     3841 tcttctgtag aggcttcatc ttcagaaagc agcgaacaag tcgaacaaac actgtggaat
     3901 gcagacaaat ccgcacaatt agaggcattt atggctacat ggggccaaac aatgggacaa
     3961 caatacaaaa gctatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
     4021 gttattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
     4081 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
     4141 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
     4201 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt taacgagaca
     4261 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atgggtaagt aaaccagtga
     4321 gagtgagaga aagaagagac tcagtggaaa atattaattg attgctatgt ctaagaatta
     4381 agatgaagga tgaaatttct agtttcgtcc ttcatcttag ttacgtttta cttggataac
     4441 tataataata acgataaata gttttctctc gacttcttta gagattgaat aaaccctcca
     4501 atactaaaat tgaatgttgc aagagcatga attgccaatt gtattattag aatttttaga
     4561 aaattgcacc tttagtttct ttagataaaa tgatacaatg attgttaaaa ttgtgaaagg
     4621 gtggtcattt gttagagaag tttttggata tgtcgttagt tcaacgtatt gttgttggta
     4681 ttttagtcgg aatattgttt ggtatctttt tccccacgtg gacatttatt tcaatattgg
     4741 ggacgttgtt tgtcagttgc ttgaaggcaa tcgcacctat tctggttttt ttcttgacga
     4801 tggcgtcaat tgccaagcat aaaattggga ataagacatt cgtgaagcct attttgatct
     4861 tatatttagt gggaacattg ctttctgcac ttgtagcagt cattgtaagt tcaattttta
     4921 ctgttcctat tactttacaa gaaacagtat ctgaaaaagc accgcaaagt ttggaaagtg
     4981 ttttatctac tatgttgacg aatgtcacac aaaatcctgt tcaatctctt attgatgcga
     5041 attaccttgg cgttttattc tgggctgtat tgttgggact agcttttcga gcacgttcag
     5101 aaccgacaaa agagttggtg gatcagatat ctatagcgtt gtcacaagtc gtacaagtga
     5161 ttattgcatt tgctccgata gggattttag gattggttta tcaatcaatt gctacgactg
     5221 gaatcgctgg attggcagag tatctgcagc ttttacttgt tttgatcggt acgatgctgt
     5281 ttgtcgcatt agttgtgtac ccattcctca cgtttttgtt tattaaagaa aatccctatc
     5341 cattgatttt cttttgtttg aaagaaagcg cacttcccgc tttctttacc cgaagttctg
     5401 ccgcaaatat cccgataaac atgatgttgg ctgaaagatt gaaattgaca aaagagtctt
     5461 attccatatc gatcccgtta ggtgctacga tcaatatggg cggtgccgcg attaccattt
     5521 ccttgatgac gttgactgct gtacattctt tagggattga agcaccattt gttttgaaag
     5581 tccttttatg tattttgtct gctttagctg cttgtggagc ttcaggtgtt gcgggcggtt
     5641 cactgttatt gattccatta gcatgtagtt tgtttggtat ctctaatgat gtcgcgatgc
     5701 aaatcgttgg tattggattt attatcggtg ttatccagga ttcaatggaa actgccttaa
     5761 attcgtcaag tgatctgttg tttactgcaa taggagagct aggcgcgcgt aaaagaagtg
     5821 gggaaaaaat caaattgaaa gattgtttag atggattaag tgaacgttga acgttagata
     5881 tttagtattt ttaaaatgat tcgagttttt aatgagttat atagatgttc gtgtaaatag
     5941 aggtttctgt taaatcaaga taaagtcgtt tataataaca attaaaagca taggtaattg
     6001 ttattggagg cgtatcatgg attttttacg taaatttggt tttttctttt tattattagg
     6061 cgtgctgagt gattttttga ctccgtacgt attaggattg tattatcctg aattagatca
     6121 aatgaagagc gtgatcagtg tatttggaga ggtttccagt cctgtaagaa aagcgtttct
     6181 tatttggtct gtggtttcag ggatcttttt catcctgtca ttgcctgcat tgtatgctgc
     6241 atttgtaagt acatctcgtg tgttagctac cctagttaca gcagccatcg cgttctatgg
     6301 tataggtgat tgtatcttta caggtttgtt cagtatcaat acccatgaat cttcgtggaa
     6361 tgtctcgaca tgggtccata atatcggatc aggtttaggt tacagtggtt ttttattgtt
     6421 tccagttctt cttttcttga tctatcgaaa aaatgggcaa atggacgtaa gccacttata
     6481 ccttggactg tctgtgatca gtttgctttt tgcggcgatt tatggactgg caagaattcc
     6541 ttctataaat tcttggccat tactgaataa aataggtttt tgccaaagaa tcagtttttt
     6601 ctttaattat gtgcccattg cctggttgag tatcttgcat ctcaaacagt aaaacttgag
     6661 ccgtcaaatt gaacagtcag cggatacttc atccggtggc tgttttttgt ttttctgaaa
     6721 tatatatttc cacagttttc ttgtatagct tctgtcttag agaagcaagg agctatattt
     6781 tgtgctacaa tcttaaaaag gtagagagaa gaccaaatgg aagacagagg agccactaaa
     6841 atatgagaaa catcaagttg acaatcgaat atgacggaaa aagatattca ggatggcaaa
     6901 gacttggaga tgacgataaa accattcaag gaaaaatcga aaaagtattg catcagatga
     6961 caaatgaaga gatagaaatt attggatcag gaagaacaga tgctggcacc catgcgcgag
     7021 gacaagtagc aaattttaaa acaaacacag aaattggact ggcagaaatg atcgacttta
     7081 tgaatcgtta ccttccgcgg gatattgtca tcaaaagaat cgaagaaatg cctgagcgct
     7141 tccattcaag atataatgcg gtgggcaaaa aatacagcta ttatgtttgg aacaacgtga
     7201 ttccttcagc atttgagcga aaccacagct tttatttccc tcaagagttg gatatggaca
     7261 agctgaatgc agcttgcgaa aaattagtgg gtacacatga tttcattggt ttttctgcat
     7321 tgaaaaaatc aaaaaaatcc acagtgcgta cgattgatga attatctatc gaacgaaagg
     7381 gagaaatgct tcattttact tttgtcggag acggattctt atataaaatg gtgcggatta
     7441 tcatggggac attactagaa atcggcacag gtgccatgcc gatagaaaca atcgatgaaa
     7501 tatttgaaag caaagtgcgg caacatgcag gagaaaccgt ccctgcacaa ggtctgtttt
     7561 tggatgaagt ttattatcgc tcataaaaaa gaatcatctt tactcgactt ctgagaattg
     7621 gagaacatcc cttttttcag aggtcgtttt ttagttaaac caaaccatag agtatgcttt
     7681 ttaagataaa gatcatctta tatcatctga agctgcttat cttctttgaa taaatacgat
     7741 tcaatcgatt atttcctcac tttatgataa attacagcta aagtcactag cttgtcttct
     7801 tcggaaagga ggagaatcta tggatgagga acaaatattc attcaaacac tttataatct
     7861 gattttgaat ccgaatacac gagattggga acgaaaagta ttgattcaga caaagaatga
     7921 cacaagagaa aatatatccg ttaaagaaca gttatcaaaa ctagaagtaa cactacgtcc
     7981 attagctatc cgaatgaatc tgacgcctga tgtaatggac ttttatttat tgattacgga
     8041 aggatttgat aaggagcaaa aatatgactt ttccaagcat gccatgcaag atgctgacta
     8101 tcaagaacgt gcagtatttg cagggggatg tttctggtgt atggtcgagc cgtttgaatc
     8161 gaaaaaaggg atcctatctg ttttgtcagg atacacgggc ggacatgttg aaaaaccgaa
     8221 ttatgatcaa gtaagtggtg gttacacagg tcatgtggaa gctgtggaga tcatttatga
     8281 cacaagagaa ataagttata gcgagttgct gacaatctat tggcaaatta ctgatcccac
     8341 agatacattt ggacaatttc aagatagagg gaagcaatat cgtccagtga ttttttatca
     8401 agacgaacga caaaaagaat tagcggaaca atcaaaacag aaactagaca gttcaggaac
     8461 tttccaccaa ccaatcgtca caaaaatcga accagccggt acgttttggc cagcagaaaa
     8521 ctatcatcag caattttaca aaaaacaacc gaaacgctat aaaaaaatcc agcaagcacg
     8581 taatcaattt ctgatctatc aacgagtaaa aaacaaatgg caaaaaaata tacgaaaaaa
     8641 tcattttgat taactccttt tctttaaaat gtagtaaact aaaaatagaa aacaagaaag
     8701 cgttttaaaa agatttgatg aggaggagaa aaattgtata ttgcagatag tgcacgttat
     8761 gaaaaaatgg aatatcgtaa atccggtcat tcaggtttaa aattaccgat tttatctctc
     8821 ctctcggatt atggcaaaat tttggtgatt atgatcctat ccataatcaa agagagattt
     8881 taagaggcgc attcgacatg ggaattaccc atttcgacct tgcgaataat tatggaggcc
     8941 ctgctggagc tgcagagaaa aactttgggc gtatttttag agaagatttt caagcctacc
     9001 gtgatgaact gattatttct agcaaagcag gttatcatat gtgggaaggg ccatatgggg
     9061 aatggggctc tcgtaaatcc atcatatcaa gttgtgacca aagtttccaa agaatggggc
     9121 tagattatgt tgacatcttc tatcatcatc gcccagaccc tgatacacct ttggaagaaa
     9181 cagcagaagc gttgatgcaa cttgtaagac aaggaaaagc attatatatt ggtatatcca
     9241 actataatgg agaagataca aaaaaatgac tgaaatttta aaaagaaagg aagcaccgtt
     9301 cattattcat cagatgcgct acaatatgtt ttctcgtgca ctactagaag acgatctatc
     9361 tccagtatta gaagaagaag ggctaggggc aatcacgttc agtccgcttg ctcaagggtt
     9421 gctgacaaat cgttatctac atggtattcc tgaagattct agagcacatc gtaaggaaat
     9481 accattcctt tcagaagaac aagttggatc aactctggaa aaaatcaaag cactgcaaac
     9541 aatcgccgta tcacgaggac aatcattagc gcaaatagcc cttgcatgga atttacgaca
     9601 aaaatctgtt acaagtgtac tgatcggagc aagtcggtta agtcagttac aagaaagtgt
     9661 tcgaatgatg gataatttag atttctcacc agaagaagaa ttgagaatcg accaaattct
     9721 agaatagaaa cgatgtaatc aaacttttta tccatatatt aaaaaaaaca aaagatgaca
     9781 tcaagaatcg aatgaagatc gtctgttatt gaacatcata ttttttgtcg atcaaaataa
     9841 aaatgatcga aggctaggag tgaattttat cctagtcttt tttatggtaa atatcttctt
     9901 cgtttgtatg tctatgaacg gattaatcga ttttttatac aaaaaatcat cagatgattc
     9961 aaacgtgtta taataattac gaaaaaatac atcagatgaa aaggaggtat gtggtttgga
    10021 agaaaataaa accctagtag aagtaactac tgacaagatc cttagatatt taaaggaaat
    10081 gcagttgcaa gtaggagata aactgccgaa tgaatatgaa ttgtccgatt atttagaggt
    10141 ggggcgaagc acggtgcgag aagctgtgcg ggctctagct tccaggaata tccttgaagt
    10201 tcggcaagga gcggggacat ttgtcagtga taagcgaggg attgccaatg acccactcgg
    10261 tttttctttg attgaagatc aggatcggat gatacgagac ttatttgaac tgagatattt
    10321 attggagcct aagatggcag gtttggcagc ggaatatgca acgaagaatc aaatcgtcca
    10381 aatgaaaaaa ttaacgcaaa agattgaaac ttcttttcga gaaggcacac aagaacatgt
    10441 tcaattagac atcgattttc atacgatgat tgcagaagct agcggaaaca tagccttgct
    10501 gcatattatc ccaatcatta atgaatcgat tgctttgttt aataaaaatt atgatatcca
    10561 gcatctgaaa atcgagacga tcgatattca tacagagatc gttcaggcca tcgaaaggcg
    10621 gaacgtactt gctgcgtttg acgcaatgac tgtccatatg gcaataaatc ggagagaatt
    10681 acagaaaata ataggagaaa aagaaactaa ttgacagttc atctgatgta ttttatgata
    10741 aatacatcag atgatttttt gttttatatt tggtaaaaaa gaggagtaaa tagtttctgt
    10801 tcaccaaaat gtaagcgctg actaatagac aaaaagagat gacgtcaagg agaagaagca
    10861 ggaatgaggg gatctggaat gagttttatg gataaattag ccaaagtttt agggaacgtg
    10921 gcaacgagaa tcaataattt aaggtatatc atggtgataa aaaacgcatt tgctgcattg
    10981 attccggtaa ttattacggg tgcttttggc acattgtttt cagcaatggt ctttgactcg
    11041 gaaaatggct tagcacagat aagttacttg cgttttttag aagaattaaa gcctatcgca
    11101 tctgccatca gctatgtcac actaagtttc ttgaccatct atgctgtttt tctaataggg
    11161 atcgaattag ccaagctgaa taaagtggat ggtgtattcc ctggtatcat cgcagtgatg
    11221 tcttatttat ctgttaatcc aactgtttat gaattagtga atgaaaatca gactgttata
    11281 gtagaaaacg tattggctaa acaatataca gacacgaaag ggttattttt gggaatgttg
    11341 gtctccatcc tttctattga actttattgc tggttgagga aacagaagaa actacagctg
    11401 agaatgcctg ataccgtgcc aacgaatgtt tctgctagtt tttctgcttt attcccgacg
    11461 attctgactg tatcgggtat cgcggctgcg ggatttatca tcaaagaact tacaggaatg
    11521 tacctttatg atatcattta caatgtcgta caacgcccat tagaaggtgt cgttcaaggg
    11581 ttaccgggta ttttgctatt gatgtttatt gcgcaagtct tttgggtgat tggtatccat
    11641 ggaaatcaaa tggtgaagcc gatacgggaa ccgttgcttt tagcggcaat tgctgtaaat
    11701 acggaagctt ttgaagctgg aaaagaagta ctgaacatta ttactatgcc attttgggat
    11761 atgtatatga gtatgggtgg gtctggagtc acgatcggtt tactgattgc agtattgtta
    11821 gttggaaaac gggaggatat gaaacaaatc accaagttat cccttgctcc tgggatcttc
    11881 aacattaatg aaccagttat ttttggtatg ccgatcatgc tgaatccaat tttagctatt
    11941 ccgtttatct taacgcctct tatcacagga acgattggtt attttgcaac cgctttaggg
    12001 tttgcggcga aagcagttgt aatggtccct tggccaatgc cgccgatcgt caatgcttat
    12061 ctggcaacag caggaaatat cggtgcagtt attacgcaac ttgtttgtat tattgtttct
    12121 attttgatct atctgccatt tgtaaaagta gcaaatcgtg tggaaaatga tgggatctaa
    12181 aaacaggagg aaatcatgaa aattactatt ccaaaaaatt ttattcttgg tgctgcatct
    12241 tctgcttggc aaacggaagg ttggaaagga aaaaaagaag gacaggattc gtttatagat
    12301 agctggtata aaaatgaaca ttttgtttgg cataatgggt atggcccagc cgtagcaact
    12361 aattttatgg aacaatacca gcaggatgtg gatctgatga aatccatcca tctgacacat
    12421 tatcggactt ctatcaattg ggctcgtttt tttactgatt atgagaactg tatagtagat
    12481 gaagaatatg ctcagcatat aagtgaaatg atcgactctt tgatcgaagc agacgtagaa
    12541 ccgatgattt gtttggaaca ttatgaagtc ccagcttttc ttttggaaaa atatgatggc
    12601 tggtcttcaa gaaaagtagt tgatttttat gctaagtatg ctcaaattgc ctttgtggtg
    12661 ttagtatcaa atcttagaca gcttttcgta gagacaagaa taaattaaac ttatctacga
    12721 gaggatgaat ttttatgacc cgatatgaag aaaattttaa acaaatgatt
//
LOCUS       DACCWK010000069        12737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCWK010000069 DACCWK010000000
VERSION     DACCWK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.069"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IVW65_001891"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IVW65_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7900763.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="IVW65_001892"
     CDS             complement(323..892)
                     /locus_tag="IVW65_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7900764.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="IVW65_001893"
     CDS             1078..1980
                     /locus_tag="IVW65_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7900765.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IVW65_001894"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IVW65_001894"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7900766.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IVW65_001895"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IVW65_001895"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7900767.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IVW65_001896"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IVW65_001896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7900768.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="IVW65_001897"
     CDS             8548..8733
                     /locus_tag="IVW65_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7900769.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="IVW65_001898"
     CDS             complement(8795..9991)
                     /locus_tag="IVW65_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7900770.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="IVW65_001899"
     CDS             complement(10161..11597)
                     /locus_tag="IVW65_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7900771.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="IVW65_001900"
     CDS             11890..12699
                     /locus_tag="IVW65_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7900772.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4011 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaat
//
LOCUS       DACCWK010000070        12537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCWK010000070 DACCWK010000000
VERSION     DACCWK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.070"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            37..774
                     /locus_tag="IVW65_001901"
     CDS             37..774
                     /locus_tag="IVW65_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7900773.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            788..1603
                     /locus_tag="IVW65_001902"
     CDS             788..1603
                     /locus_tag="IVW65_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7900774.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1616..2263
                     /locus_tag="IVW65_001903"
     CDS             1616..2263
                     /locus_tag="IVW65_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7900775.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2279..2935
                     /locus_tag="IVW65_001904"
     CDS             2279..2935
                     /locus_tag="IVW65_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7900776.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3097..3918
                     /gene="racE"
                     /locus_tag="IVW65_001905"
     CDS             3097..3918
                     /gene="racE"
                     /locus_tag="IVW65_001905"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7900777.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3921..5276
                     /gene="rph"
                     /locus_tag="IVW65_001906"
     CDS             3921..5276
                     /gene="rph"
                     /locus_tag="IVW65_001906"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7900778.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5277..5795
                     /locus_tag="IVW65_001907"
     CDS             5277..5795
                     /locus_tag="IVW65_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7900779.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5902..6402
                     /locus_tag="IVW65_001908"
     CDS             5902..6402
                     /locus_tag="IVW65_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7900780.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6682..7602)
                     /locus_tag="IVW65_001909"
     CDS             complement(6682..7602)
                     /locus_tag="IVW65_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7900781.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7767..8780
                     /locus_tag="IVW65_001910"
     CDS             7767..8780
                     /locus_tag="IVW65_001910"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7900782.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8890..9645)
                     /gene="fabI"
                     /locus_tag="IVW65_001911"
     CDS             complement(8890..9645)
                     /gene="fabI"
                     /locus_tag="IVW65_001911"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7900783.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9865..10305
                     /gene="fabZ"
                     /locus_tag="IVW65_001912"
     CDS             9865..10305
                     /gene="fabZ"
                     /locus_tag="IVW65_001912"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7900784.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10420..11181)
                     /locus_tag="IVW65_001913"
     CDS             complement(10420..11181)
                     /locus_tag="IVW65_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7900785.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11370..12464
                     /locus_tag="IVW65_001914"
     CDS             11370..12464
                     /locus_tag="IVW65_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7900786.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4041 a         2175 c         2641 g         3680 t
ORIGIN      
        1 agaaatctta ctaaataata atgggagtgt aattctatgg caatgattaa atttgatcac
       61 gtggataaat attatggcaa gttccacgca ttaaaaaata tcaatctcga attcgagaaa
      121 ggagaagttg tcgttgtcat tggtccttct ggttcaggaa agagcacgat gcttcgctgt
      181 atcaatggat tagagacgat ctcttcaggt aagctgctga ttaatgatac tgacttgcat
      241 gataaaaaga caaaactgac agaagtccga aaaaacatcg gcatggtttt ccaacatttc
      301 aatttgtacc caaataaaac tgttctggaa aacatcaaat tagcaccaat caaagtgttg
      361 aagcaagacc aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac tgtcaatatg
      421 ttagataaga aagattctta tccttccatg ttatccgggg gacaacagca acgggtggca
      481 atcgccagag gtttagcgat gaatcctgag atgctgctgt ttgatgaacc tacttctgct
      541 ttggatccgg aaatgattgg tgatgtatta gacgttatga aaaaattggc tcatgacggt
      601 atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc ggatcgtgtt
      661 atcttcatgg ctgatggaga agtattggaa gacagcagaa atgtgagaga ctttttcgaa
      721 aatccaaatg aagtccgagc acagcaattt atcagtaaag ttatcaatca ttgataggag
      781 gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc cttttaggca ttgcttttct
      841 tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag agcgaagcaa
      901 atctaccaat gaaatcatct ggggtgtcaa gtatgatact cgattatttg gaatgatgga
      961 tattgagagc agaactgtac aaggatttga tgtagatatt gcaaaagcaa tcactaaaaa
     1021 gatccttggt gacaatggaa aaacagaatt tgttgaagtc acttctaaaa ctcggattcc
     1081 attattgaaa aacggaaata tcgatgcaat tattgcaacg atgacgatca cagatgaacg
     1141 aaaaaaacag gttgactttt cagatgtcta tttcgatgct ggccaagctt tgttggtaaa
     1201 gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct tctacaacgg ttctagcagt
     1261 caaaggttct acatcggctg cgaatatccg acagcatgca ccagatgcaa aaattttaga
     1321 attagaaaac tatgcagaag catttactgc tttacaatct ggtcagggag acgcgatgac
     1381 tacggataat gcgattttgt tagggattgc agatgaaaat ccagagtatg agttagttgg
     1441 tggaacgttt actaatgagc cttacgggat tgcgatcaac aaagggcaag aaaacttctt
     1501 gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat ggcacatacg ataaaatcta
     1561 tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag taagggggag aggcaatgag
     1621 cgaattgata caaacttatg gaccaatctt tgtcgacggg ttcaagatca cattgttttc
     1681 gagtgtctta gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa tcttccaatt
     1741 atcaaagaat aaagtagtga gcgccttagc aaaggcatat gtagaatttt ttagaaacat
     1801 tccattatta atcatcgtga tgttttttta tgttgtcgta ccgatttatt ggtttaatct
     1861 agatggattc caagcaggta cgattggatt aacgatctat acatctgctt ttattgctga
     1921 aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa acagaagcag gactatcttc
     1981 tggttttacc tattcagaaa tcatgcgtta tattgtttta ccgcaagcat tcaaaatcgt
     2041 tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta ttttagcaat
     2101 cgtagctggt ttagatttga tgtatcaagg agacttgatc gctagtgaaa cattcaatac
     2161 cttcgatacg tatatcatcg ttgggttatt ttacttagtt atcacgctac cgttatctta
     2221 cttaatggtt tatcttgaga aaaaatgggc ggttcgttca tagaaggagg ggaaatacat
     2281 ggattttagc ggcgcttttt catggatgaa tatccgtttt ttattggaag gattaaaggt
     2341 tactgttgaa gtttcattgt tttcgatcat ttttagtttc ttgatcggtg gtctgacagg
     2401 tgtgattcgt tttgcgaata tccctttctt ttccaaaatc cttggtgtag tgattgatat
     2461 tatacggaat cttccattat tattgatcat tttcttcact tactttgcct tacctcaaat
     2521 cggcattcag atgaacatct tctggtccgc tgtcgcagca ttaacgattt ttgagtcagc
     2581 tatgttgtcg gaaattttcc gagctggact caatgctgtt ccaaaaggac agatggaagc
     2641 aggattatca acgggactga cttatgtaga atcgatgcga acgatcgtgt taccgcaagc
     2701 atttaaatca atgatcccgg caattgtgag ccaattgatt tcactgatca aagatacttc
     2761 attagcagta atcatttctt tacctgaact ttctcatcag gcacgtatta tttatggaca
     2821 gaacaccaat tatgtgcttc cgatgttcat catcatgaca cttatgtact tcgtggtatg
     2881 ttacgcgctt tctctgcttt ccggttactt ggaaaagaga cggtacagtt attaatagat
     2941 gattctttta gcggttgtgg caaactaaag tcacaaccgc ttttcctctt tccattcgaa
     3001 aaaaagagat ttcgattgtt ttaagcaagt aagagcactt gaaaattcac gctaaaaagt
     3061 acacaatagg gaggatagaa tttattgaaa aaaaggatga tacgattgac agataatcgc
     3121 cctatcggat ttattgattc aggtgtcggc ggcttgactg tagtaaaaga agccctgaaa
     3181 caattaccga atgaaaatat tttatttgta ggagacacag cacgctgccc atatggccct
     3241 agacccgcgg aacaggtaat ccagtatact tgggaaatga cggattatct ggtggagcaa
     3301 ggaatcaaga tgctggtgat cgcctgcaat accgcaactg cggtggcttt agaagaaatc
     3361 aaagctgctc tttctattcc agtcatcggt gtgatccttc ccggtactag agcggcagta
     3421 aaaaaaacac aaaataaaca agttggcatt atcggtacga ttggtacggt aaaaagtcaa
     3481 gcttatgaaa aagcactgaa agagaaagta ccagaattga ctgtgacaag tcttgcttgt
     3541 cccaaatttg tttcagttgt cgaaagtaat gaataccatt catcggtggc gaaaaaaatt
     3601 gtggcagaaa cattagctcc tttaaccact aaaaaaatcg atacattgat tttgggatgc
     3661 acccattatc cattattacg ccccatcatt caaaatgtaa tgggagaaaa tgttcaactg
     3721 atcgattctg gagcagaaac agtaggtgaa gtatctatgc tgttagatta tttcaatttg
     3781 agcaattcac cgcaaaatgg tcggacatta tgccagtttt atacaactgg ctctgccaaa
     3841 cttttcgagg aaatagctga agactggctt ggaatcggac acttaaatgt agaacatatc
     3901 gaattgggag gaaaataagg atgcgtcatg acgggagaaa agcccaagag cttcgcaaac
     3961 tggagcttca aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc cgttttggtg
     4021 atacaacagt tatttgttct gcaacaatag aaaattctgt tccccctttt ttgagagaaa
     4081 caggaacagg atgggtgaca gcagaatata gtatgctgcc tcgagcaacg agtacgagaa
     4141 atcgtagaga aagcagcaaa gggaaacttt ctggtagaac aatggaaatc cagcgtctta
     4201 ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt aggagaacgt agtatcatcg
     4261 tagactgcga tgtgatccaa gcggatggcg gtactagaac agcaagtatc actggggcat
     4321 ttgtggcatt gaaattagcc attgaaaaac ttttaagaga aaaagaattg tcagaagatc
     4381 caataaaaga acatcttgct gcagttagcg taggaatctt accagatggg acttgcgtca
     4441 cagatttgga ttaccaagaa gattccgcag cacttgtaga catgaatctt gtaatgacag
     4501 aatctggaaa attcgtggag attcaaggaa ctggagaaga agcaacgttt gatggtgaac
     4561 agttgaatga aatgctcttt tttggaaaaa acgcgattga agatttgatc aaagagcaaa
     4621 aacatgcttt gctaacagag tttgctcaaa atgatgagcg gatagaagaa acaaaaacaa
     4681 tcatcattgc gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg ttcaaagaag
     4741 cgggatatca tgtaaaaaca ttacttgact atcctgaatt gccagatgtg gaagaaactg
     4801 gcagcacatt tgaagaaaat gcccgattaa aagcagaaac gattgcacaa ttgcttgatc
     4861 agccagtctt agcagatgat tcaggtttga aagtggatgc tttaggcggt atgccaggaa
     4921 tctattctgc acgttttgca ggggaacaaa aaagtgatgc ggggaataac gccaaattat
     4981 tatatgaatt gacagatgtg ccggatgaaa aaagaacagc acaattccat tgtacgttag
     5041 tattcgcggc acccaaaaaa gacagccttg tagtagaagc ggaatggccc ggtcgagtag
     5101 cccgtatacc atctggcgaa aatggctttg ggtatgatcc tttatttatc ccagaaggta
     5161 agaaacaaac agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt catcgggctc
     5221 aagcaatgaa aaaattgagt gccgagtgga aacaatggct ggaaggagag agatagatgc
     5281 ggtatttagt agtaagtgat aatcatggag acagagatat cctcgttgac ttagtagatc
     5341 gttatcgttc tgaagtggat atctttttcc attgtggtga ttcggaatta gaagcttctg
     5401 acccattatg ggatacattc caagtggttc aaggtaattg tgattatgga ccaggattcg
     5461 aacagaaaaa agtgatccaa actggtcagg atacagtatt catgactcac ggtcatttat
     5521 cgaatgtccg atttggtttg acacagttag caatcgaagc tcgagctgca actgccgata
     5581 tggtgttttt tggtcatacc catcaaatag gctgtgagat ggatcagaaa atcctgtttt
     5641 tgaatcctgg tagcatttct cagcctagag gcccgatcca aatcccatca tatgctctta
     5701 tcgaaagttt agatgaccaa atttctgttc aatactataa ccgtgcccat caggcagttg
     5761 aagagttggc cttttctttt tcgagagagc gctgaataaa aaaactctaa tttttaataa
     5821 aaataagctt tattttatga attttgcatc cggtttcggt aaaatagtag tgttgaatga
     5881 aatagaaatg gaggccatca gatgattgga cctatagtaa gagaaatgct gctggaaaat
     5941 caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca tcatccattg
     6001 tcccatggtt tacttgtgtt atcgaaggtc ggttattcca aaattcctgt attaggaaaa
     6061 gatgatcact ttgtaggact agtaagttta tcagatgtgg tcaataaaat gattgacttg
     6121 caaacaatca gcatggaccc gttagaagga ctaacggttg ctgatgtgac agaaacagat
     6181 gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt agtcgattcg
     6241 ccttttttac cggttgtcac agaagatcgg attttcaaag gaatcattac aagaaaagag
     6301 ctgcttaaag ccgtaaactt catggtgcat gaattagaac gtcgtaatat attgtctccg
     6361 aaaatcgaag aattaaaaga acaaatcgat atcgtgagct aaagaacgag aaactaaaag
     6421 ataatcaaaa aataagtaga aaaaacagcg taccagtttt gactttatct tgaaaaagag
     6481 gctgggacaa aagcgtttaa ctccaagaaa taagaaggaa ttcacgaaaa ttgcttttca
     6541 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc cgtttatacg
     6601 tgtttagagc gtgggacaaa agtgttcttt acttttgtcc cacgctcttt ttagtgagat
     6661 tcgttgcatc gtttgtccaa attattttcc gacagcaatt ggactgttcg gaatcgtgaa
     6721 accagaagct gtcaattctt tgacatagga gctcaagaac tcttctttta aggcatattg
     6781 ttttccgttc aatgcgtagc agaccgtacg aatcgcaaaa ttgctgtttc caagatccac
     6841 taatccaaag atagaaggtt cagtttggag ctcttcttgg tatttttcag ctaatgtacg
     6901 atttactcga tcaatgatct catagatttt gtcatatcct tcttcaggaa caatccgaat
     6961 atctaaaagt acttgcatat ttgcacgaga gagattgctg atcgtggtga tgtttcgatt
     7021 cgggatgaaa tggacagtac catccgttgc tttcagctga agggtacgga taccaacaga
     7081 tacgacggtt ccttcaatcg ttaagtttgc tagcttgatg tagtccccaa catccatctg
     7141 ttgttccatg ataataaaaa atccggtaat aacatcactc atgaaccctt gggcaccaag
     7201 accgattgcc acacctgcaa ttccggcacc agccaaaagt gatccgatcg gcacaccgat
     7261 agcagataaa attgcataga taaaaaagaa accaagggta taatgaaaaa tagtatttaa
     7321 aagactatgt aaagtcttta agcggctctc gtttagaatg gcttttttag aatactgatt
     7381 aaaagactta tcaatcagat attttcccac acggtgaaga atccaaaaaa ggacgatcaa
     7441 aaaaatcaga tataaacttt tttggataaa ggcagccaaa atggcgtccc aatcaatccc
     7501 gtcccaaaac cgctgaaacg catttacttt gctgacagct tgatctgtca atgtctcgcc
     7561 agaagagtct gtcgtaccgg cagttgctaa taatgaaaac ataaaatcct cttttctaaa
     7621 atttgattct ttctgtattg taccaagaag ttttcaaaaa aggaaatagc aaaaataact
     7681 ataatgaagg aaaaaagctg aaaataaaat gacaaataat atggataatg ataaaatgtg
     7741 gagaaaataa cgaaggtggt aagtatatgc aaatcgattg gaacaatcta ggtttttctt
     7801 atatcaaaac tccttggcgt tttattgcca aatggaaaga cggacaatgg gaagaaggag
     7861 aattgacaga agataattac ctgacgattc atgaaggctc tccagcgctt cattatggtc
     7921 agcaatgttt tgaaggtcta aaagcttatc gtcgaaaaga cggcaaggtc aatctctttc
     7981 gacctgaaca aaatagtcga cggatgaatc aaagtgctag acgattgctg atgccaatgg
     8041 tccctgaaaa gatgttcgtt gatgctgtca agcaagtggt aaaagcaaat gaagattttg
     8101 tgccgcccta tggaacaggc gctacattat acttgcgccc tttactgatt ggggtcggtg
     8161 aaaacattgg tgtccatcca gctccagaat atttatttgt cgtcttctgt acgccagtag
     8221 gagcgtattt caaaggtgga ctaaaaccaa cgaattttat tgtatctgat tacgatcgtg
     8281 ctgctcctaa ggggactggt gcagcaaaag tcggcggaaa ttacgcagcg agtttacttc
     8341 caggaacaga agcaaaagaa ctggattact cagattgtgt ttatcttgat ccagccacac
     8401 atacgaaaat cgaagaagta ggtgcagcaa atttctttgg aattacaaaa gacggaaaat
     8461 tcatcactcc gcaatcgccc tctatcttac caagtatcac gaaatattcc ttgttgcagt
     8521 tagctgaaga acgactagga ctgcaagcaa ttgaaggaga tattttcatt gatgatctag
     8581 atcaatttgc agaagctggt gcatgtggta cagcggctgt tatttctcca atcggtggga
     8641 tttatcacaa taacaatctt catgtatttt atagtgaaac ggaagtagga ccagtaacaa
     8701 aacggttgta tgatgaattg acaggtattc aatttggaga catagaagca ccagaaggct
     8761 ggattgaagt cgtggaataa tttcaaagta atgagttgaa aaaaacgtaa aaagaaaaaa
     8821 tcaggaagag actttaaaaa gttttttcct gatttttata tgttttactt taaagaatgc
     8881 aacaaaagtt tatgaaagat ggacaccttt gtcaacgtag ataatatcac cgatgattcc
     8941 gctggaaagt gggctgatta agaaagcaca agcattaccg acttcttcga tcgtcacacc
     9001 ttgctgatca ggcgtacgag attcagataa ctggatcagt ttttgatagt ctttaacacc
     9061 tgtgacagct aatgttttga ttgccccagc tgagattcca ttgacccgaa ttcctttagg
     9121 cgagaactca gctgccaaat aacggatagc agcttcaaga gaagctttcg caatacccat
     9181 catgttataa ttcgggattg cgcgttctga acctagataa gtcatgctaa cgatcccgct
     9241 gtttggtgca agaaacggtt gtgcgtattt tgatacagca attaaagagt agctgctgat
     9301 atcttgagct aattgatagc cgtctctgga aatatcagac acatttccgg acaattcttc
     9361 tttgttggcg taagcaactg catggacaag gccgtggatc tccccagcat attcaccgat
     9421 ggcattcatt gctttttcga tgctttcgtc gctagaaaca tcacattcta cagtgaatgc
     9481 ttcttcgccg accaatttga ctaatgattt tttcattcgt tcattttggt aagtatagat
     9541 aatttctgcc ccttgtttca acattgcttc agcacaaccc caagcaatgc tttttttgtt
     9601 agcgacgccc ataatgacga ttttttttcc ttctagaaat gacataatga ctctcctttg
     9661 tttctgataa aaatttgata gtcgtaaaaa aatagagaat aatagctaaa ataacttctt
     9721 gatttcttaa ttattagtca aaatgttttg attgtcaaac tattttttct taaattaaaa
     9781 aaagattgct aaatatttag gaatatgata ctttgatagt caaagtaatg aaaatggcaa
     9841 ttgcctgaaa gaaggagttt aattatgtct tacgtattat ctgctactga agtaatggaa
     9901 ttgattccta atcgttatcc tatttgttat attgattatg tagatacttt agaaccagga
     9961 aaaaagatcg ttgcgacaaa aaatgtaaca atcaatgaag attttttcca aggtcacttt
    10021 cctgacaatc cagtgatgcc gggtgcgttg attctagaaa cactggcaca agcaggttcg
    10081 atacttatcc taaaatcaga agagtttcta ggaaaaagag cttatatcgg tgggatcaat
    10141 aaggccaaat tccgtcaaaa agttgttcca ggagatgtgc tgaaatgtca ttttgaaatc
    10201 accaaaatga agggatcagt cggtacagcg ttatctgcag catatgtaga tgataaaaaa
    10261 gtctgtgagt gcgagttcac gttcatcgtc aaccaaaatg cttgactata aaacctgata
    10321 tagcaaaaca aaaggaagct tacttgcttt ttatgtaaaa agaagacgcc tgaatcctag
    10381 catacgttgt catctatgca ggattcagac gttatttttt tatttttgtt ttgcttcttg
    10441 cagtatattg attttatcga tttttacttc ttcttttggt ttgtctgaac tatctgtttc
    10501 gatatttgca atagcatcca cgacatccat tccttcaatc acttggccga agacagtata
    10561 atggccatct agtgaaggat aaccgccgtt tttataagcg tcgatgattg cttgaggata
    10621 gtcgtctttt aaaagaccat catgcatatc atctgtattt tgaacgataa agaactggct
    10681 gccgttactg ttaggatctt gtgagcgagc catagataac gcacctctta tattataaag
    10741 gtggttgttg atctctgttt caaatccttc tccccaaatg ctttcccctc cagtgccatc
    10801 gcctttagga tcgccacctt ggatcatgaa atctttgatg acgcgatgga aagtcagtcc
    10861 gttgtagtaa ccgtcttttg catgtgtcat aaagttttca accgcttttg gtgcgtgttc
    10921 gggaaataat ttgattttga ttgtgccttt attcgtcacc atttcgacca gatcttcatt
    10981 ttcggcgaca tctttcgtca attgtggtaa ttccaatgcg ttcaaatcga cgctactggc
    11041 agtcgtgctt tccgcagtag atgtactttt tgaactttct gtagacgaac tagaagaagt
    11101 cgtgttgttt gcagaacaac cagcagcaaa cagcaatgct gctgctaaac taatacttaa
    11161 aataacttta tttttcttca taactgttcc tttccaattt actattttgt tatctttaat
    11221 gataacaaaa tagagggaaa atacctactt aaattattgg aatttatcat ttattttaaa
    11281 agaaaaagaa aaggtgtaga ccatttactt tgtgtttgct ataatgtaag tggatacaaa
    11341 aacgaaaaga tgcaggagga aatggtaata tgggaaaatt aggaatctcg atttatccag
    11401 aacggtcaac atttgaaaag gacaaagcgt acttggattt agctcataag tatgggtata
    11461 aacgggtttt taccagtttg ctgcagatca atgatgataa agaaaaagtt ctttcagaat
    11521 ttaaagaagt agttgattac gcgaatagcc taggcatgga agtaatggtt gatatcaatc
    11581 cagcgttatt tgaacagttg gagatttctt acgatgactt atcttttttc cataagatgg
    11641 gtgcctacgg ggtccgctta gatatcggat tcacaggagc agaagaagca aaaatgacgc
    11701 gcaatccttt tggtatcaaa atcgagatca atatgagttc gggcacgaat tacgttgaca
    11761 acatcatgag ttattcgcca aacacggata atttattagg ttctcataat ttttatcctc
    11821 atcgatactc tggtttagga tacgaacact ttgttttttg ttcagaaaaa ttcagaaaat
    11881 ataatttgaa tacgatggca ttcgtcaatt cgcaatctgc tgagtttggc ccttggccta
    11941 cgcaagatgg cttgtgcacg ctggaagacc accgagatct tgaaattgcg acacaagtga
    12001 agcatttaat ccttacagga ttgattgatg atatttctat tggaaatgcg tacgcaagtg
    12061 aagaagaatt gaaagaaatg gcagaagcat ttaacgcgga ttatccaaca ttgaaagtag
    12121 atacggaaga aggtattaca gaaaacgaac gaatctgctt gtttgataac cttcatagtt
    12181 atcgagggga tcgctcagaa tatatccttc gttcaacgat gactcgtgta tactacaaag
    12241 ataaagattt cccgcctcat aatacgcgag atatgcatca tggggatgta ctgattgaca
    12301 atgaagggta cggtcaatac aaaggagaaa cacaaatcgc cttgaaggat atgaaaaacg
    12361 acggacgtgt caatgttgta ggaagaatct ccgatgacga attattcctc cttgactttt
    12421 tgaaaccatg gtcaagcttt aaattaattg aaaataaaaa atagataatt agttattgca
    12481 gaaataatcc gctagaaata gccagttatt tctgcttttt tattttgttt ttttata
//
LOCUS       DACCWK010000071        12453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCWK010000071 DACCWK010000000
VERSION     DACCWK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.071"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(129..776)
                     /locus_tag="IVW65_001915"
     CDS             complement(129..776)
                     /locus_tag="IVW65_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7900787.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1055..1558)
                     /locus_tag="IVW65_001916"
     CDS             complement(1055..1558)
                     /locus_tag="IVW65_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7900788.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1681..2445)
                     /locus_tag="IVW65_001917"
     CDS             complement(1681..2445)
                     /locus_tag="IVW65_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7900789.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2552..2827
                     /locus_tag="IVW65_001918"
     CDS             2552..2827
                     /locus_tag="IVW65_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7900790.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3504..4454
                     /gene="yidC"
                     /locus_tag="IVW65_001919"
     CDS             3504..4454
                     /gene="yidC"
                     /locus_tag="IVW65_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7900791.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4633..5607)
                     /gene="lacD"
                     /locus_tag="IVW65_001920"
     CDS             complement(4633..5607)
                     /gene="lacD"
                     /locus_tag="IVW65_001920"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7900792.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5604..6062)
                     /locus_tag="IVW65_001921"
     CDS             complement(5604..6062)
                     /locus_tag="IVW65_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7900793.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6059..7522)
                     /locus_tag="IVW65_001922"
     CDS             complement(6059..7522)
                     /locus_tag="IVW65_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7900794.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7504..8454)
                     /gene="pfkB"
                     /locus_tag="IVW65_001923"
     CDS             complement(7504..8454)
                     /gene="pfkB"
                     /locus_tag="IVW65_001923"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7900795.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8597..9346
                     /locus_tag="IVW65_001924"
     CDS             8597..9346
                     /locus_tag="IVW65_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7900796.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            9613..11091
                     /locus_tag="IVW65_001925"
     CDS             9613..11091
                     /locus_tag="IVW65_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP7900797.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(11159..12250)
                     /locus_tag="IVW65_001926"
     CDS             complement(11159..12250)
                     /locus_tag="IVW65_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7900798.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         3878 a         2527 c         2104 g         3944 t
ORIGIN      
        1 aagaaagggg ctatccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg acacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctggac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtgt tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgatctactt ctctttttat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgttcatt cttctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaaccg agaaaatcag ccagattttc
     1021 tcggaatata gtctattatt attggtttta cctattaaat cgaggattct ttcccaaagt
     1081 aacgttgcca tctcttacgc atacttgcag acatgggctg agccgcttct ttgactgcgc
     1141 agtatttgtc cacgaatgtc acaatatatc cttctttata cttaggcggt gcgatcgttg
     1201 caccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg gtgatttttt
     1261 ctgcattctt tacagcaatt cttggatgga tatacgcatg tgtcccctcg tcaaatttcg
     1321 tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct gttgctttag
     1381 cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg atggaatgct
     1441 ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact gcttctgttt
     1501 ctaaaagatc ttgtacataa gacatatatt cttcgtcttc acgccatttt tctgtcataa
     1561 ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta agtataccac
     1621 cagtgttcgg attattctag tcgctctaga taaaatagct aaagcttaat agttccttaa
     1681 tcataaagaa aaatgataaa gaacgatccc tgctgcgact ccgacattca atgattccgc
     1741 atttcctttc attgggatat atacgttctg atcagtcaaa gacaagattt cctgacttac
     1801 tccctgacct tcgtttccca tgattatagc gaaatcctct gttttttcta ctttgtctaa
     1861 tgcatgtgct tcttcattta attctgtacc gtacaccgta aaccctttgc ctttcattac
     1921 agggattaca tctgccaaat ctttttgtaa cacaggcaaa tgataattgc ttccctgcat
     1981 gcttcgcagc acctttgtac tataaatatc tgccgttcct ttacctaata aaacaccagc
     2041 cataccaaac gcatctgccg tacggatcat cgttcccaca tttccaggat cctgtacatt
     2101 atctagtaaa agccagcctc ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac
     2161 tgctaagatc ccttgcggtg taggtagatc tgacatagcg gtcatgacct catctgaaac
     2221 tagaaaagta gaaatctctt tgtttacagc cagccagtct ccccattctt tcatacctct
     2281 ctgtgtcaca aaaagttctt ttatttctgc attagacgac gcagcttctt cgatcagatg
     2341 gaacccttcc aaaagatatt ctgccttttc ttctctgtat ttctttttat gtagtttttt
     2401 taattctttg attcttggat tttttgctga ttggatctct ttcacattcc tcacctcaac
     2461 tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg
     2521 atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg
     2581 acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc agttaggcgt
     2641 gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga
     2701 cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa gtggacgagt
     2761 gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat ttgatgtagt
     2821 gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt tgtgaaaatg
     2881 gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt tgaacaccgt
     2941 cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat tttcaaaata
     3001 gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata aaacaccgtc
     3061 aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt gataaatagc
     3121 tctccatatt tttcaacagg ctggacttaa tgccgtagat gcaagctttt gaacaccgtc
     3181 gataaggttg tttttgaggc gctttgcctc aagtattaaa gacaggtagt aaaaaagctg
     3241 atctataact tgcaatgaat ttgcttaacc catattttcc agacagaaca aaaaagtttt
     3301 tagtttttta aataagaaag aaacacttgg aatcactctt ttctttctaa gtatttcttt
     3361 cttgattttt tgtctttttt caaaattttt cgctacgaaa aagaacgaat atttatatga
     3421 aatttctatg aagttgattg aattccatgc gcttttttag tatagttaag tttgtgtaaa
     3481 aattatagaa gaggaagtat tctatgaata aatggaaaaa ctggttattg ggatccggac
     3541 ttgtcagtat cctgctcttt ttatctggct gtgtgcggat ggattcaaac ggaaaccctg
     3601 acacttcagg aatcgtttat cgagtactcg tccatcctat gggccaagcg attacatatc
     3661 tagttgagaa tttcaattgg tcttacggct gggcagttat tgcaatgact gtgatcgttc
     3721 gtatcatcat cttgccacta ggtatcagtc aatcgaaaaa aacaatgatc caatctgaaa
     3781 aaatgcaagc gttaaaacca caagttgaag cagcacagca aaagctaaaa gctgcaacaa
     3841 cacgtgaaga acaaatggca gcacaagcag aaatgcagca agtatatcgc gaaaatggat
     3901 tgagtatgac cggcggaatc ggctgtctgc ctctattgat ccaaatgccg atcttctctg
     3961 ccttgtattt tacggcacgc tacacagaag gtatccggga atcatctttt tacggcatcg
     4021 atctaggctc accaagtatg gtgcttgtgg ccatcgcagg ggtcgcttat ctgcttcaag
     4081 gatatatttc aacaatcggt attcctgaag aacagaaaaa gacgatgcgt acgatgctga
     4141 tcgtctctcc tgctatgatc gtcttcatgt caatcagtgc gccagccggt gttacacttt
     4201 actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt catcacaaac gttatgatga
     4261 aaccacgttt gaaagcacaa gtggcagaag agttgaaaca aaacccgcca aaacaagtcg
     4321 tcactccaag aaaagacgtg acagaatcag caaagccagc taacaacaaa caagcaacaa
     4381 aacaaatcaa acaaccaaaa aacagtacaa acaacaaagg ccgcaacgca ggaaagcagc
     4441 agaagcggaa gtagatagta aataagagtg taataaaaat tcattaacag ttttagcaat
     4501 taaaaatatc gaccaagatt tttgaaaaag aaatctggtc gatattttta tttaagtgaa
     4561 tgtttaattt gaatcatcga ttgatcgcta tctaattgca gcagcatctt tttatgaaga
     4621 aacaactttt tcttatctat caacgtagac aggtgttgct gtttcttcta atagtgtact
     4681 taattcttta atattttgtt ttccttcttc taacatccaa ctactcgctt tttcttcgcc
     4741 ttcggtaaca aaaggtgcca ctcccttcgc ccatgttgct cgcccacata agacaccatt
     4801 gaatgtcgaa cctgattgtt ttgcaaaacg taatgtatct tgaaatagcg tagcattgac
     4861 tcccgcactt aaaaaaataa acggttgttc agtcactttc gattgttcat agaaatacgc
     4921 ggatgcctct gcttgactat aacaagtctc ttgcccatat ccttccacaa atctcatatt
     4981 cactggcact tctactttca atacatccac ttgataacgt tcttttgaaa attctcgcat
     5041 ggcttcaata actttatgtg gttttacttt tgcataggca agagaagacg gatcgccaat
     5101 agttgcatca taagaaagaa tctctaaaaa gaaaggtatg ccttctgctt cgcattctga
     5161 accaatccgt tctataacta tttgtttttg cacattaatc tctgctggtt catcaacgtc
     5221 atagtacaat aaaaatttac aagcatccgc accagcttct tttaaccttt tcaccgacca
     5281 aatattcaat aaatcaggta atcttcccgg tattttcgta tcatagccgg ttttttcata
     5341 agctagcagt aagccacatt ctttctctcg aagcgctgct gcaggcaggc cgtattctgg
     5401 atcaagtaaa atagctgagg catagggagt caggtattga gaaaccatct gtttaaattg
     5461 gatgatgtct tctgatgtag cttctttata ttggttgatc attttcttca aagcaccacg
     5521 ctggtcgatt gctaatgcgc taataattcc agcttcatcc gtcaatcgct tcaaacgttc
     5581 ctgtttttct atactcatct tcttcataga tcttcaccta tttctttagt taatattgtg
     5641 gctatttctg tttttgattt tgcttttctc aactgttctg taacctcttt tcgcatcaat
     5701 gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc ctgcttctga atctggaata
     5761 aataacgcaa aaataaaatc aactgactgt ctatctaaac tgttccattc aattcctttg
     5821 ttcagtgtgg cgataatcat actaggttcg ttgattgttt gagacttcgc atgagggata
     5881 gcaaaacctg acattattcc tgtagtccct tctttttctc tttctagaag tttttgatat
     5941 acttcttttt cgtcaagagc gatccctaaa cagaatgttt gctttgctaa aaacttaaat
     6001 gcttcttctt gactatccaa tggctgttgt aaaaaaatgt tctcttcatg aatgattgtc
     6061 atgataagtc tcctgctctt cctttttcga taactgtacg atttgctgct ttattatcca
     6121 tcttcttgtc tttatcagtt actcttttct tttggattaa tcctagtatc actccaccaa
     6181 cgatcgaacc aataagaatc gctgtaaccc attggatttt tcccgtcact acaggtaaca
     6241 ctaaaaaacc accatgtggt gctggaactt ggacgccaaa caaataagtt aagacagcag
     6301 caattgatga gcctaacatc atcgtgggta acactcggat cgggtctttc gcagcaaacg
     6361 gaatagctcc ttccgtaata tgagttgatc ctaaaataaa attaaccaaa cctgcattac
     6421 gatcattggt atcaaaatat ttaccaaaca ataaagtagc aaaaccagta atcaacggtg
     6481 gtgcaataca agctgctgac acaccagcca taaaagtcgt gtttccttgt gccaaaagag
     6541 ctgttcctgt tacatatgcc gccttattaa ccggtcctcc catatcaaac gcacacatac
     6601 agccaacaat cacgccaaga acaagtggat tcgaatgttc aaatccagct aaaaagctca
     6661 tcattccttc atttatagcc ttcattggac ctgccagcaa agacatcacg atgccaataa
     6721 atgctacacc aataactgga tataagaaga ttgccttcaa gccatctagt tgtttaggta
     6781 aaattgtcag tagcttttgc aaaaataaga taaaataacc tgctaagaac ccagctaaaa
     6841 tacctcctaa aaatccagta ccgccattca tggctacgat tccaccaaca aatccaacga
     6901 ctaaccctgg acgtttcgcg atcccttctg caatataagc tgctaaaact ggaaccatca
     6961 ttcccattgc tgcatcgcca actgctttta ccattgccgc tgtagcattg tattgtgcat
     7021 gatctggatc aaaagaatag attccccaaa cggcaaatga aatagcaatc aatactccgc
     7081 cagctaccac gaaaggcaac atatgagaga caccgttcat taaattttta tagatgctac
     7141 gaccaaaatt agcaccactc atcgcttctg gttctaaagt atcagcagct tcattatctg
     7201 ctaagacagt accttttcct gccaatgctt cgtcaatcag ccgttgtgca tccttgattc
     7261 ctgtactgac agaaacatca atgactcgtt ttcccgcaaa tctttctgaa tggacatctt
     7321 tatctgcggc aataatgaca gcatctgcct gtttgatttc ttcagccgtt aattcatttt
     7381 cgatcccaat ttgtccatgg gtctccactt tgatcttgat gccttttttc ctagctgctt
     7441 gttctaatgc ctcttgcgcc atgtaagtat gcgcaattcc tgtcggacaa ccggttgctg
     7501 caattagttg atattttccc atgtttcaaa cctctcttcc ttgattttga tttgtttact
     7561 taattcctcc acatccaaaa aatcagtcag tcctttacga aaagcggttg agctgcctgc
     7621 tgctatgctt ttacgtaacg tttcatcaag cggtcgtcga ttcatatatc ctgctaaaaa
     7681 agcgcctaac atggtatcac ctgcacatgc tgtattgaca acttttcctt ttggcgagtt
     7741 tcctcgaaac accctgtctt tcgtaaaaag aatcgctcct ttctcaccga gggataacaa
     7801 aacattttct gcacctaact ctactagtcg ttgtccataa acaaagtaat cttcatctgt
     7861 aaccattttt cgtccgaacc aagaagaaag ttcttcctcg ttcggtttca gcaaaaacgg
     7921 tcgatagggt aaacaatcca tggcttcttg atcactacta tcaataatca aatgaatccc
     7981 cttttcctga caaatccgac tgatttctat taaaaccttt ggagacagcc ctcttggtaa
     8041 actcccagaa acacaaagat aatcttctgc ttgtaatgct cttatttgat ttaaaaaatt
     8101 atggacagct gtctgcggta ttttaggtcc ttgattcact aatttgtatt cttctcccgt
     8161 ttgattgact tgtgtaaata cattgatacg cgtcatacca ggcacttcaa taaactctgt
     8221 ttggatttgt ttctgctgta aatagtcttc tatatacttt cctgtaaacc cagcagaaaa
     8281 acctaatgcc gtacttggga aatctagcat ttttaatact agtgacacat tcaccccttt
     8341 tccgtttgcc tgaatgtcat cctcttttgt gcggtttacc ataaaaggat gcatctcctc
     8401 tgtttcaata aatagatcaa ttgccaaatt catcgtgcat gtgtagatag acataaccaa
     8461 cgacctcctt gactctatta tgaaatacgc gaaagagaaa atgttttctt aatcgtccca
     8521 acacaaagaa aacctttaca tttataaact cgtcgtgcta ttttttaact aacttttggt
     8581 gaaagaagga aagaatatgg atcacaaatt aagtgaaaca gaacagtacc tctggaactt
     8641 tatcgaacac cacatcctag agatccctaa ctactccatc gtgaaactaa gtgaacaagc
     8701 aaatgtctca acagccacca ttgtgcggac aatgaaaaag aaaggctatg aaggatttac
     8761 ttcgtttaag caccatctaa aagaaggaga aaacaagaac atcaattttt ctttcttaga
     8821 taaagtagac aaaggtatcc gtcgagccat tctaaaaaat gaacaagaag tggtacgtac
     8881 gatcaatatg ttggaaatcg gaaatattga ggatgcaata caaagaatca aatttgctga
     8941 ccgggttttt gtctttgcac gaggattttc agaaatgatt ggtcaagaga tgctcgtaaa
     9001 acttcaatta acagggaaaa attgtcaaat gcatactgat ccagaaatca tcaaaagcat
     9061 cagtcggaaa ctaactaaga aagacagcgt tatttttgtt tctttgaacg gcgagacaaa
     9121 agaactcgtc accgccgcaa agaattgcta tgatgcagag attggttcga ttctagttac
     9181 agccaatcat tcctctacac tgaaagacta ttgcgagatc aatctcgtag gtttcaaatc
     9241 agaaggttct tatttccctg attatgaagt acggtcacgt ttgcctctcc agattatagc
     9301 gagaatcctg cttgacgcct atgtattgcg tatgggggag aattgaaaag cagtttatta
     9361 tggttggtta cagtttaaaa catcacattt tttattcttt atattgtatg gactcacgga
     9421 aactttttat gtataaagtt attcacaatc tataaagtta ctttaaataa caaaaaaata
     9481 attgaaaata cttaaaatca aaaaatatct ttttaatcaa taaaaaaatt gacagtttta
     9541 ttaataataa gctattctgt tatagaagaa aaaattgttt gttattatta gcaacaaaaa
     9601 aaggagtgca tcatgaaaaa gttatctcaa aaacgtttag ctcaaatcat tattgattct
     9661 agaaaatctc tgaaaatgac acaacaacaa cttgcagtag ctactggtat caaccgtgcc
     9721 ttaattagtc gattagaatc tctcgatttt atcccgtcaa tacaacaatt agaatcatta
     9781 gcagaagttc taaattttga accaacagat ctatttataa atgaatcaaa taaatcaaac
     9841 agaacaaaac atcagtcgaa gaatattgct gtagccggta ctggttacgt aggtctttcc
     9901 attgctactc ttcttgccca gcacaatcag gtaacagcag tagacattct tcctgaaaaa
     9961 gtaaaacaaa ttaacaataa gaaatctcca atacaagacg attacattga aaaatatcta
    10021 gctgaaaaaa atcttaattt aattgcaacg actgatggtg cagccgccta tagagaagcc
    10081 gattttgttg tcattgctgc tccaacaaat tatgatagta aacaaaatta ttttgatact
    10141 tctgctgtag agtcagtaat caaattggtg ttagaagtca atccaaaggc aataatggtc
    10201 atcaagtcga cggtacccgt aggctatacc gaaagtattc gtaaaaaata caatactaaa
    10261 aatattctat tcagcccaga atttctcaga gaatctaagg cattatatga caatctatat
    10321 ccttcgcgaa ttattgtcgg cacggaccaa actgacaaaa ctttacgaga agcctctcac
    10381 acttttgcag atttactcca agaaggagca atcaaaaaag atatcgaaac tcttttcatg
    10441 ggattcacag aagctgaagc agttaaatta ttcgctaata cttaccttgc acttcgagta
    10501 tcttacttca atgagctgga tacttatgca gaaatgaaag gactaaatac acaagaaatc
    10561 attcacgggg tttgcttaga tccaagaatt ggcacacatt ataataatcc atcttttgga
    10621 tatggaggct attgtttacc gaaagataca aaacaacttc tcactaacta taacgatgtt
    10681 cctcagaata tgataagtgc aattgtcgat agtaatcgta caagaaaaga ctttatagca
    10741 gatcgagtgt tagaacttgc tggcacgtat gaggctaaca gttcgtggaa tgcagaaaaa
    10801 gaaaaagagg tagtcatagg tgtataccgt cttacaatga agagtaattc agacaacttc
    10861 cgtcagtcat ctattcaagg agttatgaag cgaattaaag caaagggggc aactattgta
    10921 atttatgaac caacacttga aaataacact acattttttg gttcaaaagt aataaataat
    10981 ttagatgatt ttaaaaatat cagtcacagt attattgcta atcgttacga tagtaatctt
    11041 gatgatgtaa aagaaaaagt atataccaga gatttatttc aacgagatta aaaaataaca
    11101 cctcagattt gtagtgtctg aggtgtttct ataaactttt ataagtaaaa aaatacaatt
    11161 atacttcata aaaagactta taccactgcg caaatttacg aagcccgtca cgcaaacttg
    11221 tacttggttt aaattcaaaa tctcgttcta acgcactagt atctgcataa gtaactggca
    11281 catctcctgg ttgcattgga actagttcgg tatgagattc aaagtcgtaa tcttctggta
    11341 gcacccctgc tgcaattagc tcttgttgta gaatatctac aaaatctagt aggttttccg
    11401 gcgtattgtt accaatatta taaatatgat atggtgggat tggtagccca tcttccccat
    11461 caattttctc tggagcgcac tgcataaccc tctttactcc ttcaacaata tcatctatat
    11521 aggtaaagtc acgcttgcaa ttcccataat tgaatatttg gatattatca ccagcacgaa
    11581 gtttatcagt aaaaccaaaa taagccatat ctggacgccc agcaggacca tatactgtaa
    11641 agaaacgtaa ccctgtagac ggaatattgt aaagtttaga atatgcatgt gccattgatt
    11701 cattactttt cttagttgct gcatacaacg atacttgatt gtctacttta tcctctgtag
    11761 aataaggaat tttcttatta gtaccataaa cggaagacga tgaggcatac actaaatgtt
    11821 ccacaccaga ttctccatta tcatatgaat gacgacaagc ttcaagaata ttatagaatc
    11881 ctattatgtt agattcgata tacatatctg gattagtaat tgaataacgg actcctgcct
    11941 gagctgctaa attcaccacg atacttggtt tataattatc aaagatgaaa tcaattgttg
    12001 ctttatcagc aatagaacct ttgataaaat tccaagtaga tcctggatgg ttctttgcct
    12061 ccgtttctat ctctgataat ctatagttct taatagagac atcatagtaa tcattcatat
    12121 tgtcaatgcc aatgattttt attgatgact gagtacgtaa taactcaaga accaaatttg
    12181 atccaataaa accggctgcg ccagttatta atattgttct atttgataag gacgtgttta
    12241 attccagcat tttttagcac tcactttctt tttttcttat gaatacatat ttgttatata
    12301 ttaaaaaatc ttatttagac ttatctattg tcacttttat cattctaatc tttgcacgta
    12361 gtagaataat tatacagata ggaaattgct aagcaaccca cttctttcta ttatctatta
    12421 acttattcta aaacgaatag aaattattct tga
//
LOCUS       DACCWK010000072        12110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCWK010000072 DACCWK010000000
VERSION     DACCWK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.073"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            474..725
                     /locus_tag="IVW65_001927"
     CDS             474..725
                     /locus_tag="IVW65_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900799.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IVW65_001928"
     CDS             759..1037
                     /locus_tag="IVW65_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900800.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IVW65_001929"
     CDS             1634..1891
                     /locus_tag="IVW65_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900801.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IVW65_001930"
     CDS             2004..2615
                     /locus_tag="IVW65_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900802.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IVW65_001931"
     CDS             2794..3351
                     /locus_tag="IVW65_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900803.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IVW65_001932"
     CDS             complement(4050..4571)
                     /locus_tag="IVW65_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7900804.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="IVW65_001933"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="IVW65_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7900805.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="IVW65_001934"
     CDS             8651..9538
                     /locus_tag="IVW65_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7900806.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="IVW65_001935"
     CDS             9535..10704
                     /locus_tag="IVW65_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900807.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="IVW65_001936"
     CDS             10728..12083
                     /locus_tag="IVW65_001936"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7900808.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         4036 a         2031 c         2658 g         3385 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa
//
LOCUS       DACCWK010000073        12095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCWK010000073 DACCWK010000000
VERSION     DACCWK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.074"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(31..588)
                     /gene="frr"
                     /locus_tag="IVW65_001937"
     CDS             complement(31..588)
                     /gene="frr"
                     /locus_tag="IVW65_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7900809.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(591..1313)
                     /locus_tag="IVW65_001938"
     CDS             complement(591..1313)
                     /locus_tag="IVW65_001938"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7900810.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1449..2330)
                     /locus_tag="IVW65_001939"
     CDS             complement(1449..2330)
                     /locus_tag="IVW65_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7900811.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="IVW65_001940"
     CDS             complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="IVW65_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7900812.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3570..4049
                     /locus_tag="IVW65_001941"
     CDS             3570..4049
                     /locus_tag="IVW65_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7900813.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4266..5357
                     /gene="phnW"
                     /locus_tag="IVW65_001942"
     CDS             4266..5357
                     /gene="phnW"
                     /locus_tag="IVW65_001942"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7900814.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5350..6135
                     /locus_tag="IVW65_001943"
     CDS             5350..6135
                     /locus_tag="IVW65_001943"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7900815.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6481..8172)
                     /locus_tag="IVW65_001944"
     CDS             complement(6481..8172)
                     /locus_tag="IVW65_001944"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7900816.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8594..9541)
                     /gene="arcC"
                     /locus_tag="IVW65_001945"
     CDS             complement(8594..9541)
                     /gene="arcC"
                     /locus_tag="IVW65_001945"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7900817.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9656..10675)
                     /gene="argF"
                     /locus_tag="IVW65_001946"
     CDS             complement(9656..10675)
                     /gene="argF"
                     /locus_tag="IVW65_001946"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7900818.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10766..11995)
                     /gene="arcA"
                     /locus_tag="IVW65_001947"
     CDS             complement(10766..11995)
                     /gene="arcA"
                     /locus_tag="IVW65_001947"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7900819.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3303 a         2529 c         2181 g         4082 t
ORIGIN      
        1 atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc
       61 agcagcgatc ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc
      121 taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc
      181 gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac
      241 atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac
      301 atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc
      361 ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat
      421 tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa
      481 aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc
      541 tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc
      601 cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt
      661 aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc
      721 atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca
      781 tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg
      841 ataacatctg cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac
      901 gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct
      961 ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc
     1021 ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac
     1081 atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc
     1141 cagatatttc cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg
     1201 atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct
     1261 aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt
     1321 cctccaatat tttctattgc cattttacca tacttagatg atttcgacac tctaagactg
     1381 gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt
     1441 caacagaatt attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt
     1501 tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca
     1561 acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt
     1621 tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg
     1681 ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga
     1741 atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt
     1801 gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata
     1861 tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag
     1921 ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta
     1981 tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct
     2041 gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag
     2101 tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct
     2161 gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact
     2221 gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg
     2281 ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc
     2341 tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca
     2401 gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa
     2461 catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta
     2521 cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt
     2581 taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc
     2641 agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa
     2701 tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga
     2761 taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt
     2821 taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga
     2881 tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga
     2941 agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac
     3001 ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat
     3061 ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga
     3121 tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt
     3181 ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt
     3241 tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat
     3301 aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg
     3361 tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa
     3421 agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat
     3481 aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca
     3541 tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta
     3601 aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc
     3661 attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg
     3721 tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc
     3781 ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga
     3841 aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat
     3901 ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg
     3961 atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc
     4021 accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt
     4081 gtttttttat tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac
     4141 atcatttttt attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat
     4201 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
     4261 atcatatgta taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg
     4321 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
     4381 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
     4441 aaggtagcgg ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
     4501 ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc
     4561 tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga
     4621 ttacacagct gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga
     4681 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc
     4741 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac
     4801 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
     4861 cttttgtact tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt
     4921 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
     4981 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
     5041 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa
     5101 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
     5161 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
     5221 acggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
     5281 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
     5341 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
     5401 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact
     5461 gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
     5521 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
     5581 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
     5641 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
     5701 attggttcca cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa
     5761 aaagaagggt attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga
     5821 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
     5881 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
     5941 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
     6001 ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct
     6061 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6121 aagaatccag aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa
     6181 gtaataagaa cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6241 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac
     6301 ttttgtcaca gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa
     6361 acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct
     6421 attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc
     6481 ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt
     6541 cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt
     6601 atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga
     6661 cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac
     6721 cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc
     6781 tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc
     6841 tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt
     6901 caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc
     6961 caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac
     7021 ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat
     7081 caaaccaaat ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac
     7141 agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt
     7201 gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt
     7261 cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat
     7321 ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc
     7381 catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc
     7441 atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc
     7501 ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg
     7561 ttctgtttca gctacttcat ggaattggac atataaacga agcaattcat tgattggttc
     7621 tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg
     7681 tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat
     7741 gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc
     7801 aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc
     7861 ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt
     7921 catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat
     7981 tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga
     8041 aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc
     8101 gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc
     8161 tttattattc atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct
     8221 ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc
     8281 aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac
     8341 agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt
     8401 cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt
     8461 tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc
     8521 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8581 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8641 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8701 tagctgcctc tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt
     8761 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8821 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8881 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     8941 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9001 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9061 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9121 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9181 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9241 ccttcagtac ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg
     9301 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9361 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9421 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9481 ttgcatccgt agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca
     9541 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9601 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9661 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9721 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9781 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9841 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9901 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
     9961 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10021 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10081 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10141 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10201 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10261 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10321 gttcctctac cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta
    10381 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10441 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10501 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10561 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10621 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10681 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10741 agtaagaccg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10801 tgcagcgtgg accaccacgg cctcgtgaca attcacttga attgatttct aacactttga
    10861 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10921 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    10981 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11041 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11101 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11161 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg
    11221 ctaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11281 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11341 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11401 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11461 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11521 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11581 cgtatagact gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat
    11641 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11701 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11761 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11821 atgtctttgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11881 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca
    11941 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12001 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12061 attttgtctc attcaaaatg agacattcct cacat
//
LOCUS       DACCWK010000074        11983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCWK010000074 DACCWK010000000
VERSION     DACCWK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.076"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="IVW65_001948"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="IVW65_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7900820.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="IVW65_001949"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="IVW65_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7900821.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="IVW65_001950"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="IVW65_001950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7900822.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="IVW65_001951"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="IVW65_001951"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7900823.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /locus_tag="IVW65_001952"
     CDS             6047..6346
                     /locus_tag="IVW65_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7900824.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="IVW65_001953"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="IVW65_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7900825.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /gene="rpsR"
                     /locus_tag="IVW65_001954"
     CDS             6934..7170
                     /gene="rpsR"
                     /locus_tag="IVW65_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7900826.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="IVW65_001955"
     CDS             7313..9286
                     /locus_tag="IVW65_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7900827.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="IVW65_001956"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="IVW65_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7900828.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="IVW65_001957"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="IVW65_001957"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7900829.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4001 a         2184 c         2650 g         3148 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgtaaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatca aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggtgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacat tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatggggaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc cgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaatcc ttgaaaacta tatcgaacat caacgcgaag tcatcacacg acggacggtc
     4441 tttgaaaaag aaaaagcaga agcaagagca cacatcttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa
     4561 gcaacaatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgtacag ccaaaggaat cccaatcatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggtcagag ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaaaatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata accatcaaga
     5821 ctaagtgtgt aagctagcaa gaataaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatta gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattagatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa tgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatattgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    11881 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    11941 atttcacaaa aatttgagaa gcaattttcg tacattcctt ctt
//
LOCUS       DACCWK010000075        11851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCWK010000075 DACCWK010000000
VERSION     DACCWK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.077"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(7..978)
                     /locus_tag="IVW65_001958"
     CDS             complement(7..978)
                     /locus_tag="IVW65_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7900830.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1684..3063
                     /gene="brnQ"
                     /locus_tag="IVW65_001959"
     CDS             1684..3063
                     /gene="brnQ"
                     /locus_tag="IVW65_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7900831.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(3217..3663)
                     /locus_tag="IVW65_001960"
     CDS             complement(3217..3663)
                     /locus_tag="IVW65_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7900832.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(3690..3866)
                     /locus_tag="IVW65_001961"
     CDS             complement(3690..3866)
                     /locus_tag="IVW65_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7900833.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(4028..4456)
                     /locus_tag="IVW65_001962"
     CDS             complement(4028..4456)
                     /locus_tag="IVW65_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7900834.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            4548..5408
                     /locus_tag="IVW65_001963"
     CDS             4548..5408
                     /locus_tag="IVW65_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7900835.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(5500..5793)
                     /locus_tag="IVW65_001964"
     CDS             complement(5500..5793)
                     /locus_tag="IVW65_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7900836.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(5865..7592)
                     /locus_tag="IVW65_001965"
     CDS             complement(5865..7592)
                     /locus_tag="IVW65_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7900837.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(7806..8732)
                     /gene="trxB"
                     /locus_tag="IVW65_001966"
     CDS             complement(7806..8732)
                     /gene="trxB"
                     /locus_tag="IVW65_001966"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7900838.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(8877..10310)
                     /gene="ascB"
                     /locus_tag="IVW65_001967"
     CDS             complement(8877..10310)
                     /gene="ascB"
                     /locus_tag="IVW65_001967"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7900839.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(10310..11713)
                     /locus_tag="IVW65_001968"
     CDS             complement(10310..11713)
                     /locus_tag="IVW65_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7900840.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         3257 a         2562 c         2070 g         3962 t
ORIGIN      
        1 caaagcttat tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac
       61 agggtgccga acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt
      121 taatgttcgt tctgcatgaa caagaccact tgtcgtccct cgaggcaagt ctatctgact
      181 tgtatcccca ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat
      241 ctgagcgact gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgttcg
      301 cccccgcatg taagctaaag gtgcgatttc gattacgcct cgttccatca atcggttcgt
      361 atgatccaat ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac
      421 tttttctttt agatccccgg gaagaaagcc tagattttca cctgcttcca cagcaggccg
      481 cgtcaaaatg attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc
      541 aagaaaagtt tttcctgtac cggctggtcc tacgccaaat acgatatcat gtttggctac
      601 cgattcgaca tatttctttt ggccgctgtt ttttacccgt ataggttttc cattacggtc
      661 tttgacgatc tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat
      721 tttcaatgcc gtcacaacat cgggcgtact tacgtggatt cctcgtttga tcaattcctg
      781 taatgtccga atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat
      841 gatttctcca cgtgtatgga tggttgtttc cgttgtttcc tcaatcagtt ttatatgctt
      901 gtcgtgtgca cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga
      961 actagactct tccgtcaaaa atagacatcc cctttttttc gattaattct ttcctatttt
     1021 accatactca gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc
     1081 ataagtacca agaaacgtta aaaaactagc cgcttccatt ctctaaaggt ctcgtttgaa
     1141 taaaaaaagc tgatcatgta agctatgcat aagatagagg acaagtattc cgtctaactt
     1201 agcaagtatt ctctgggtat ttttcttgtt tcaaaaaaag aaaacaaaat atttgaaata
     1261 ttcttgcatt tcaaaaaaaa caggtgtatt ataacgtcaa caaataaata tacagcaaag
     1321 cgtcgaggaa aacagtaggc tattcagtga tttattccca gtgagtcatg taagtgagaa
     1381 atgacaataa atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat
     1441 tgtggctgcc tccattatca ggctagcgga taactttatc gaaaagcgtc cgtgaatgaa
     1501 gaagcttttt taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag
     1561 tggaaaaggg ctttttttat attttaaggt ggtggttttc atggaatctg atattagtgg
     1621 tatttctagt ttctgcaacg tttattttgt tactcaaaat gataattatt ggaggaaaaa
     1681 tttatggaag aaacaaaaac ttattcatgg aagcaattat tgatggtcag ttcacttatc
     1741 ttcgggatgt tcttcgggtc aggtaatctg atctttcctg tacacttagg tcaaatggct
     1801 ggatcaaact ggatcagcgc tggtgtagga tttgccatct caggcgctct tttcccatta
     1861 ttagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct
     1921 gtcggaaaat ggtatgctgc cttgttcttg atccttgttc atttaacgat cggaccattc
     1981 tttggtacac cgcggacagc agcaactgct tttgaaatgg ctgctaaacc ctttttgcct
     2041 gaaaaatatg attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat
     2101 ttccttactg tacatccaac acgtttggtc aaatatgtcg gaaagtattt aaactccttg
     2161 tttcttgcat tattagctat catttttgtc gtttctttat tccaacccat ggggaatcta
     2221 aaccaatctg ctgcagattc ttatcagacg aacgctttga caaatggcgt tttagaagga
     2281 tacaacacgg ttgatgcagt agctttactt gctttaagtg tcacttttgt ccatgcagtt
     2341 cgtgatcttg gttttaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg
     2401 agcgttgcgt tagaagtcat gatttatttt ggtctggtcc ttctcggtgc gatgagtttg
     2461 aataagatgt ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat
     2521 ttaggtaact tcggtgctag cttcttaggg gtaatggtca cattaggtgt cttcactact
     2581 gctatgggac tcgttgtttc ttttgcacaa gatttccata aactgttccc taaagtcagt
     2641 tatatgacat ggctgcggtt aacgactttt gtctcttttg tcgttgcgaa tgccggattg
     2701 gataatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcacttgcg
     2761 ttaatcttac tttccttaac agcaaaattc ttccaaaaga gtccatttgt ctatcaagta
     2821 acgatgttct tttctgccgt tccagccgta ttggatatgt tggcaaattc gcctgcatta
     2881 gtgagtcagc aaactgtcgt agcaagctta ctaggatttt atcatcatta tgttcctttt
     2941 gctgcattag gtcttggctg ggtcgtccca acgttgcttg gttacgctgg cagcttgctc
     3001 ttttattatg tatatcgcgt atcaggatat aaacaagaag caagtgagtt accagaagaa
     3061 taaccaaatc aaacacaatt taacataaga gatattaaac aggaaaaaaa cagctagaag
     3121 aaagaataag attctttctt ctagctgttt tttagttctt tttttacgaa cacaaaaaac
     3181 tgcggataat cactgtccgc agtttaaatg ataaaattaa gattgtaata gttctttcac
     3241 aatcgcattc acttggtttc catctgcttt tcctttgact ttaggcataa ttgcacccat
     3301 aactttgcca aattctttca ttgatgaagc accagtttga gtgattgctt cttgaacaag
     3361 ttgtcgaatt tcttcgtcgg aaagttgttc cggcatatat ttttcgacta ttacaatttc
     3421 actttttact ttttcagcta aatcgtcacg tcctgcttct tcaaactcat gcaaagaatc
     3481 acgtcgctgt ttcatttcac gagacaacac cgttagttct tcttcttctg tcaagtcgtg
     3541 accttcttta atctgctcgt tttgtataga tgacttgatc attcggatga cttgtaaaga
     3601 ttccttatct ttcgctttca tcgctgcttt catgtcatcg tttaatctac ttagtagtga
     3661 cacactacca actccaattc atcacagaac tagaattttt tgcgttttct agctgcttca
     3721 gattttttct tgcgtcttac acttggtttt tcgtagaatt cacgtttacg agattcttgt
     3781 aaagtacccg cttttgacac ggaacgtttg aagcgacgaa gagcatcgtc aagtgattcg
     3841 tttttgcgaa cgactgtttt tgacatgtta attccctccc tccgagctta aaaagtaacc
     3901 gtaattgtta ttgaaatata tctcaaaaaa caagactgtc ctgtaaatat tatatctaat
     3961 gctgttttga ccgtcaagga aatcttgctt ttttttaaaa ttatcaaatg ttattttatt
     4021 taaatcctta ttgacaattt tcacatcttc cgaaaatctc gaatctatgc ccttcgatcg
     4081 tgcatccacc tagctgttct tcgaaataat tcatcggaca catatggatt tctttcgttg
     4141 ttccgcacac tgtacagata aaatgatggt gatggccgat tttcccctga cagcaatgaa
     4201 aacgaaactt catttctcca tttaaatcgg tttcttccag taaatcgatc tctgcaaaat
     4261 catggagatt ccggtaaatc gtatcataac ttaccccttt gaattcctga ttcatatagt
     4321 caaaaacttc tttagcagat acgtagcggt tacgcttcac taaataagtt aacaatgctt
     4381 ctcttttttt agtatattta aaaccgttat ttttcaatgt ttctaatgat tgttcgatca
     4441 atgtctgttt tttcatgact tattcctcca aatcaaaatg attacgaagt acgcattcta
     4501 gagtatggta taataaacat aaaaaaaatg caagtgaggt tttatccatg tcttccgatt
     4561 ctacagaaat cgttactttt tttgtcatct ccgactctgc tggagaaact gcgacaaaac
     4621 tagcgcaagc tactatggct cagtatccta ccgttgagtt caacctattc cgcagaacgt
     4681 tcgtgacaga tgaaaaaaca ttaaggcagg cattgcaaga tgcattagaa gaaaaagcac
     4741 ttgtcctgca tacattaatc aatcaagaac tgatcgagat aacacgttct ttttgtgaag
     4801 aacatcaatt atttagtttt gatgtgatga cgccaccgat atcagaaatt gaacgtttaa
     4861 ctggtatcaa gccaattcgg caacctggtg ccctccattt attgaatgag aactatttca
     4921 aacggatcaa agcaatggaa tttgctgtaa aatacgatga tgggaaagat cctcgtggtt
     4981 ttttagaagc agatgtcgtt ttgctaggcg tatcccgaac ttccaaaacg cctttgagct
     5041 tgtttttagc taacaaaaat ttaaaagttg ccaatttacc tttgatccca caagcacata
     5101 ttcccaaaca gctttgggag atcgatccta aaaaaatcgt gggactaacg aataacccag
     5161 atattttaaa taatatccgt aaagaacgga tgcgctctta cggattaaac cctgatactg
     5221 cttattccga tatcgaaaaa attagagctg aacttgattt tgcgaatgat ctttatgaaa
     5281 aactaggatg tgtagtgatc aatgtggcat ctttatctat tgaagaaacg gcttcgctta
     5341 ttttgaatgc tttagaccta gaagatcata gttattatgg aactgaaaca acagaaaaaa
     5401 gcgattaatc atttttgtct ttttcttaaa aaacactgga gtgtgacaac gaagaaattt
     5461 cgttgtcaca ctccagtgtt tatctatttg cacctatcgt tattgatgtt ccacatgttt
     5521 gagtgtctgg ttcaaaatat ccagtacatg agaatcatca agactataca aaatcgcttt
     5581 tccctcgcga tgagacttga cgacatgatt ttctcttaat aaacgtagtt gatgcgatac
     5641 agcagactgc tccatttcca ctgcttgggt gattgctgtc acattttgtt ccccgtcttt
     5701 cagatatagc agaattttaa gacgagtggg gtcactcaac attttgaata attgactgac
     5761 tttctctact tttgattttt gcgtgtctgg caatccattc acctccaact tgaaaaaaga
     5821 ctgacttcta aagtttacca ctttttccat cagtctcaag ttttttattc ttctgtcatc
     5881 tttttgataa tcgcttcata agaggctact ttttcggctg cgtcttctgt agagcttcct
     5941 tttacaccaa tataaaactt gattttcggc tcagttcctg aagggcggat agcgatccag
     6001 ccattttctt ccaatgtata cttcaatacg tcggatggcg gtgttgttag tttttctacg
     6061 tttccttcac ggtctgttct cgtcaattct ttaaagtctt ctgtttgaat aacttttgct
     6121 ccggcaaatt ctgtcggtgc ttctttacgg aatttttcca tcaaagcaga gattttcgca
     6181 gaaccttctt gtccgctcat tgtcacagaa atcgtttttt ctgcgaaata gccataggtt
     6241 tcaaaaatcg tttgcaagcc gtcatacaac gttttgcctt gttttttata gtaagctgct
     6301 acttctgcta ataaaagcaa tgcttggatc gcgtctttat cgcgaacaaa aggtttaact
     6361 aaataaccat aactttcttc gaatccaaac ataaaagtat gtgaatggtc ttcttcaaat
     6421 tgttggattt tttcagcaat gaatttaaac cctgtcaaca cattgaacat agtagcaccg
     6481 tagtgttccg caattgctgt aggcaattcg ctggaaacga ttgatttcaa taccgctgcg
     6541 ttttctggta aagtgcctct tgattgatgt gcttccaaaa tatattgagc aaacaatgct
     6601 ccgatttggt tcccagtcaa tacttcataa gaaccatctg gcaaacgaac cgctgcgcct
     6661 aaacggtctg catcaggatc tgttgcaatc agcaagtcag ctccttcttt ttctccaagt
     6721 cgtatagcat attcaaaagc tgaatgttct tctggatttg gcgatttgac cgtagtgaaa
     6781 tctggatctg ccacagcttg ttcgggaact agtacgaatt gttcgaatcc agcttgtttc
     6841 aacgcacgtt cgcccaacat tttccctgtt ccatgaagtg gcgtgtacac aagtttcaaa
     6901 tctttcccca ttgtttcaac caattcacga tcaatcgtca ctgctttgat ttcttctaga
     6961 taagcagcgt ccacttcttc tccgatgatc gtgatcaacc cgctatgttt agcttcttcg
     7021 tcagataata cttcgacttt tagcggattg ctgacttcac ggacatattt cgtcaatgca
     7081 tctgcgtctg caggaggcat ttgtccgcca tcttctccgt agactttata tccattgtaa
     7141 gccgctgggt tgtgagatgc ggttaccatg attcccgcaa acgtcttcaa atgacgaact
     7201 gcaaaagaaa gctctggtgt tggacgcaga ctttcgaata cataagacgg gatattgtga
     7261 tgtgccagtg ttttggctgc ttccattgca aattctggtg actgatggcg tgagtcataa
     7321 gcgatagcga cacctcggcg ttttgtttct tcaccttgtg tatccatgaa acgtgctagt
     7381 ccttctgttg cttgtcttac cgtatagatg ttcattcgat tgatcccagg tcctaagatt
     7441 ccccgcatgc ctgcagtacc gaattccaac ggtgcataaa aagcatcttc aagtttttca
     7501 ggatcttcac ccagatcatt taattgtttt tttaagtttt cttctaaatt ttcttcatgt
     7561 acccattgtt catacacttg ttcccaagac atgaaaataa cacctctttc tgaaatatgg
     7621 ttcttaataa gtatagcatt cacttattag atatgaaagt atgaggacca aaaaacgctt
     7681 aaatcaagaa aggattcttt tatcaagcat gattgcaagt attcacataa atcggattca
     7741 gtggatttca cgcgaaaaaa aagagtaaaa gaacaattga aaccgttctt tcactctttt
     7801 taaacttatg ctgttgtttc ttctaaagac tccaagtatt tatacacttg ttgtccagca
     7861 ataccgccgt caccaacagc tgtagtgatt tgtcgcaaat ctttttcacg aacatctccg
     7921 atggcaaaaa cgcctggcat attcgttttc atttcgttgt ctgttggaat ccagccagct
     7981 tcgttcgtca aacctgcttc tctaaaaggt gcagttaatg gatccagtcc aacatagatg
     8041 aatactccgt ctgctgctaa atcaaattca ttgccggttt tgacattacg tgctttcaca
     8101 cctgtcacta ccatttcgtt tccaacgatt tcgtcaacga ctgtatccca cataaaggaa
     8161 attttttcat ttgcaaatgc acggtcttgg atgatctttt gtgcacgtaa ttcatctcta
     8221 cggtggacga tcactacttc agaagcaaat tgtgtcaaat agatggcttc ttctactgct
     8281 gagtccccac ccccgatgac tactagacgt ttgttacgga aaaacgcacc atcacagact
     8341 gcacagtaag aaacgcctcg tccagcaaat tcttcctcgc caggtacgcc taatttacgg
     8401 tggatacagc ctgttgcgat aatgactgtt tttccttcgt aacttttatc ttcacagatg
     8461 acttctttat ggctgccgtg atccttgata tcttgaacga ttccataagc attttctgtc
     8521 ccaaatcttg agacactttc atacatctta taagctagat cggggcccat gatcgaatcg
     8581 aatccaggat aattttcgat ttctgccgta ttgttcattt ggccgccagg ggcaccttgt
     8641 tcgatcatca atacggataa gtttgaacgt gaagcatata aagcagcagt catcccagca
     8701 ggacctgcgc caataacgat tacatcatac atatgtagat tctcctttac ttaatcaagt
     8761 aagcatactt tacagccaat gcgaaaaaaa gtctattgtt ctgcttgcgt ttctcgaata
     8821 gctttattat actcgaaaaa tccgctgaaa aacaatagac ttttataaat cattgatcaa
     8881 tcgttcgaaa gattttctcc atttgaatca attacttttt tataccagtt gaatgatttt
     8941 ttctttgaac gggctaatgt gccatttcct tggttatcac gatcgacata aatgaatccg
     9001 taccttttct tcatctcacc ggtaccagct gatactaaat cgatacatcc ccaagtcgta
     9061 tagcctaata gatcgactcc gtctagttct actgcatcct tcatcgcctg gatatgcgct
     9121 gccaggtaat cgattcggta atcatcttcc acataaccat tttcatctgg cgtatcgact
     9181 gcacctaagc cgttttctac aataaataat ggtttttgat aacgatcata aatatcattc
     9241 atcgttattc tcaagcctaa tggatcaatt tgccaccccc attcacttgc ttgcagatat
     9301 ggatttttga cagaagcaaa gatatttcct gcagtctgtt ccaagagttc gttgtcggta
     9361 gtcgaaactc gtgaagaata ataagaaaaa gagataaaat caacagtatg ttctttcagc
     9421 aatgcggcat cccctggttc catttcgatc tgaatgccat ttcgctccat ttctttcaat
     9481 gcataagcgg gatattctcc tcgtgactgg acatcaatga agaaataatt ttctcgatct
     9541 gattttctag aagcccaaac atcttcagga ttgcaagtgt atggataata tgctcctgct
     9601 gccagcatac agcctacttt gttctccgga tctacttcat gagcaatctt cgttgcgatg
     9661 gcacttgcaa ccaactcatg atgtgccgct tgatatttga tttgttcttc attttctcct
     9721 tcttcaaaat acagccctgc gcccataaat ggtgcatgaa ggatcatatt gatctcattg
     9781 aatgtcagcc aatatttgac aagtccttta tagcgattga agatgactcg acacagattt
     9841 tcataaaaac caacgagttc tctggaacgc cacgcaccat attctttgat caaatgcatt
     9901 ggacaatcaa aatgcgtaat cgtcacaagt ggctcaattc cgtgcttttg gcattcttta
     9961 aagacatcct cataaaattt caagccagct tcattcggct ctttctcatc ccccattgga
    10021 aaaatacgac tccaggcgat agacattcga taagtcttga accccatttc tgcaaacaaa
    10081 gcaatatctt ctttataacg atgatacata tcgattgcaa tttttgctgg ataaaaatga
    10141 tcctcatcaa attcaaacat ttttttcttt ccggtgataa caggaaaacg gtcttttccg
    10201 attggcacaa catctacgtt tgctagtccg cgtccacctt catcataagc gccctcacat
    10261 tgattggctg ctgtagcacc gccccataaa aaatcttttc tgaaccccat tatacttcct
    10321 ccattatatg tgtattacct gataattctc ttgccagttc atcttgaact tcgattcctt
    10381 cttcttttaa tcggctgatt gtttccttga attgcggtaa atactgctgg tgtgcaataa
    10441 acaactcatc catgatccgt tttgctgtct ttcctgatcg tataagtgga ttgatcgtaa
    10501 acgcctgtaa agctgtccca taatcaccag tcacagctgc ttcgatcgtc agcaattcca
    10561 tattcttcat tacttgcaac cagcctcttt cagcagcagg aagagaacca aacgctacat
    10621 ttctcgctcc ttgtgcgcca acataggcag tgacttctac aacacaatct gccgacaggt
    10681 caggaacagc tccatcattc tttgtagata caacgatctg tgtttcttta tttccataga
    10741 ttgaagcaat cgtttcacag gcagcgtctg aataataagc gccacctcgt ttttgcaatt
    10801 gttcaggttt atggtctaat tctggatctt tgtataattc aaatagctct gcttctgttt
    10861 ctttcacttg ctgcgctctt gtaccaatcg tttgatattc ttctaaagca tgttccagca
    10921 tttcttcttc acgatagtaa tagcggtggt agccacaagg gatcatgttc atttgtttta
    10981 attgttcttt gaaaaacggg atatcaaaga tatttttcgg taatccatta tcttcatcgt
    11041 ataaacgatc aatcaattct gccgtgcggt catttccttt attatctgct actttgtgcc
    11101 aatggaaatg atttagaccg gcaaacttgt agatcagctc ttcttttttc ttgccaagca
    11161 ttgctggttc tgtcatcatc gccatcactg gcacattgca taatccaatc actttatccc
    11221 atttgccata tctgataact gcttctgtca ccatcccact tgggttcgcg aaattgatca
    11281 gccaagcatt tggacataaa cgcttcatgt cctcaacgat tgccaaaacg actggaacgg
    11341 tacgaaaagc tttgaatatc ccgccagcac catttgtttc ttgcccaatc atcccataat
    11401 aactagggat ccgctcgtct ttgattcgtg catctaattg accgacacga aactgagtcg
    11461 taacaaaatc cgcatctttc aatgcttctt cccgatccag agttgcatag atcttgactt
    11521 catatggaga agcatcccac atacgctgag acatctgact aacgatctca agcttttctt
    11581 ttccttcttc tacatctacg agccagattt ctcgtatcgg taattcctca tatcttttga
    11641 taaatccttc catcaactca ggggtgtaac tgctgcctcc accaatcgta gcgattttga
    11701 ttccttttgc catgttctca ctcctttact tttttacact ttcattatct tttttgtaaa
    11761 caaaacaaac aatagaaatc cgtttctttc cagtaacatt tacaaaagtt actgttttct
    11821 agtggatttt tgtaaatatc ttgtcaaaaa a
//
LOCUS       DACCWK010000076        11767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCWK010000076 DACCWK010000000
VERSION     DACCWK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.078"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            272..472
                     /locus_tag="IVW65_001969"
     CDS             272..472
                     /locus_tag="IVW65_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7900841.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            619..3408
                     /locus_tag="IVW65_001970"
     CDS             619..3408
                     /locus_tag="IVW65_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7900842.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEEQMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYQEELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3455..3982
                     /locus_tag="IVW65_001971"
     CDS             3455..3982
                     /locus_tag="IVW65_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7900843.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4003..5193
                     /locus_tag="IVW65_001972"
     CDS             4003..5193
                     /locus_tag="IVW65_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7900844.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5233..6531
                     /gene="asnS"
                     /locus_tag="IVW65_001973"
     CDS             5233..6531
                     /gene="asnS"
                     /locus_tag="IVW65_001973"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7900845.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7697..8371
                     /locus_tag="IVW65_001974"
     CDS             7697..8371
                     /locus_tag="IVW65_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7900846.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECVNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8368..8709
                     /locus_tag="IVW65_001975"
     CDS             8368..8709
                     /locus_tag="IVW65_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900847.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLI
                     AVTKEKQIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTKEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8739..9098
                     /locus_tag="IVW65_001976"
     CDS             8739..9098
                     /locus_tag="IVW65_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7900848.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9622..11703
                     /gene="cadA"
                     /locus_tag="IVW65_001977"
     CDS             9622..11703
                     /gene="cadA"
                     /locus_tag="IVW65_001977"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7900849.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         3937 a         2058 c         2544 g         3228 t
ORIGIN      
        1 aaaaagaaca tcagagcaaa aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa
       61 gttcggtttt tcactttatg taatcgctga gaaaactttt tttaataaaa aaagtcgaaa
      121 aactctagct tttttgtaaa cggtatgata tactatagga gtagtttttg ttcggtaatc
      181 tgtggtagct tgtttcaaat aaacatcttc cagatataca ccattcaagt aattgcaaaa
      241 tatgtgttga tacacaagga ggatttttat tatggaaaca ggtacagtaa aatggtttaa
      301 ctcagaaaaa ggatttggat tcatcactgc agaaaacgga aacgatgtat tcgttcattt
      361 ctcagctatc caaggcgacg gattcaaaac tttagaagaa ggccaagcag taactttcga
      421 tgttgaagaa ggccaacgcg gacctcaagc aacaaacgtt actaaagctt aattaaccta
      481 tactaaaaaa taaacgccca gacatttgcg tgtctgggcg tttttgtcta ttttctttaa
      541 aaagcgatga gagtattttt ctgcactttg tagtataatt tcaaaagaac attagaggca
      601 ggagaagagg aagatatttt gaaaccaaga acttatgcag tcgttgatat cgaaacgact
      661 gggacgaatc caaaagaaga tcggatcatc caatttggct gcgtcatgat tgaatcaggt
      721 cggatcactt cacgtttttc gatcgatatt catccagggc ggaagatttc aaaacaaatc
      781 cagcatttaa cagggatcac gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc
      841 gcccaaacga tttacaatct tttggcagac accatatttg tcgcgcataa tatttatttt
      901 gattataatt ttttgaacca tgaactcatg cgatgcggac ttcctagttt gaagatacca
      961 ggcatcgata cagtagaact tgcacaaatt ttcttgccga cggaaccaag ctttcgctta
     1021 gctgatttgt cagaaagcct tggatttatt catgagaatc ctcatcaggc agatagtgat
     1081 gccgaagtga caggtcaact tcttttgctg atcgaagaac agatgagaaa attgccaatc
     1141 atcacgttag agcagattgc gcgattaagt agacacacag gtatggatac gagtcgtttc
     1201 atttatcatg tgattgaaga aatgaaagaa aaaccagagc cacttgatcc ctcgttagaa
     1261 atcgtcgatg ggctagcttt acagaaaaaa gaagtggaac tattcacatc cgtccattat
     1321 ggcgagcgga catatccaag aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg
     1381 atgtatcgaa aagaacagaa cagactgatg aatgcggtct acgaccattt cacaaaagat
     1441 gaatcaaaag atttgatgat cgaagcagct acaggaatgg gtaaaacaat cggttatctg
     1501 ctaccattga gttatttggc tacacctgaa aaacccgctg tcatcagtac cgtttcgcta
     1561 gttttgcagc agcagatcat agaaaaagac attccattgc tgaatcaatt gctagatcag
     1621 cccatacaac ctgtaattat caaaagctat cgccactata ttgatttaca gcgttttaaa
     1681 ggcacacttg tggaaccgcc agaacaaaaa caatatgctt tatatcaaat ggcggtctta
     1741 gtttggctga cgcaaacgaa gaccggtgat ttggatgaat tacatttaac aaatttgaac
     1801 cattcattct ttgcagatat cgcgcacaga ggtattggat ttttagcaag aaatcagtca
     1861 ttttacgaac aagactttgt tcgtttcttg caaaaaaaaa tcaggcaaag taattttttg
     1921 attgtcaatc atgcgttttt agtacaagaa acacagcgaa aagaaccatt gcttccagct
     1981 agtccatttt tgttgatcga tgaagtacat catttgccgg aaatcgctga aaaagtaaat
     2041 gacaaacgat taagtattaa ctatttccat aaacaaatcc agcaattaga agaaccaaat
     2101 cagctgtttg accgaataca aagctattta ccgaaagaac atgaagcctt acgctggtta
     2161 tcgatttatc aagaagaact ttctgcgcta tgcgaagcac agatttgtat ggcagaagga
     2221 ttaatgagtt tagtgagcgg caagcaacct aacggacagt atccagaaga agtcatgatg
     2281 acaaaagaga tgttggatca gctgccactg gaatctggga cagccattga tcacctgcag
     2341 cttttttatc aggagatcca gcagctacag tcacaaatgc gtctgacttt tgagattatc
     2401 caagaaagct ggacaaacca gatgcggatc gatttagcaa gtttatatag cttatttgat
     2461 caattagaca agcagtactt gctgatggat caatggctgg aagactggcg agatcatttg
     2521 atccattggt tcgttcctgg gaacacagcg cagcagatga tttttcatct ccatgatttt
     2581 caagcggcta tgttgccgtc gactgtttgg tatcctcgat atgatcgtat tctttatatt
     2641 ggaggaacgc tgaagatcgg ccctaatcga cactatctct ctaagaggct tggcattgaa
     2701 gcagcacctc ttaaagtgat tgcctctcct tatgattatg agaaacaggc aaggatctat
     2761 gtgccaaaag aggcacctgc cgtccatcaa gtcagcaatc aagcatatat cgattattta
     2821 gagacagcac ttacaggtct gatcgaacaa gaagatcggc caatacttgt gttattcact
     2881 tctcatgatg ttttgcagaa ggtctatcag aaaatgcatc tgtctatgct agaaaaagga
     2941 cgagaaatac tagctcaagg gtttggcgga agccgagaga agcttttgaa acgcttcatt
     3001 ttgtcagaaa aaagcgtatt gtttggcgct gacagctttt gggaaggtgt agatttgcct
     3061 ggagatgctt tgcagatcgt tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg
     3121 atggtcaaag ctaggaacgc ttatttagaa tcagagggga tcaatccttt ttaccaggaa
     3181 tccgtaccta aagcagcttt gaagttacgc caagcattag gtcgcttgat aaggacggag
     3241 acagatcgtg gcgtattgct tattttggat cgaagactag tcactgcaaa atatggtgca
     3301 cggatcatcc atgcattacc gaaaaaagta ccgatcaaag agctagctat ggaggaaatc
     3361 tatcaggaca tccatgaatt cttaaagaaa aatgaagaag gaagttagag gagctgtctt
     3421 ttttcataat taaaatctgc tataatgaaa aggaatgaag ataaaggagc gacgaccctt
     3481 gaagaaaaaa cagacaaagg aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt
     3541 tttattagcg gtcatcgtga tcagctcgat tttttacttg cgttccagtc gtccggcggt
     3601 gcaggcgaag aaagaagcag tagaaatcgc aaagaaatat gcaaatcttg aggaagccga
     3661 tcatttttat tggtttactc gaaagaagac atacttttca ctgacaggta caaatgataa
     3721 aggaaaagaa atcgccgtta tcattcctaa atctggtgaa aaagtaaaag tattggatca
     3781 aacaaaggga ctgactgaaa acgaagcaaa acagacaatt gctgcggccc atcctgaaat
     3841 ccaaatcgaa aaagctgctt tagggatgta tgatgatcag cctgtgtggg aagtagtagg
     3901 aaaagaaaca aatgggaaac tgaattatta cctgattggc tttgaatctg gagatgaagt
     3961 caagacaatc aagggaattt aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga
     4021 acaaaacgat tagaaccgtc tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa
     4081 gccaaaggca gagatgtgct gagtttgact gtgggagaac cggattttgc gacaccagaa
     4141 aacatccaag aagcagcaat cgaagcaatc cgcaatggga aagctagcta ttatacacca
     4201 acagcaggaa tcccagagct tcgacaggca atcgttgatt atctgaagaa aaactatgga
     4261 cttgtctatg aaccttcaca aacagtcgta acagacggag cgaaatttgc tttgtatacc
     4321 cttttccaaa cgatccttga cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc
     4381 agttacggtg agcaagtaaa actggcagaa ggacttcctg tctttttgaa aggagaagaa
     4441 tccaatagct ggaaagttac tgttgagcag ctagaacaag cacgttcaga aaaaacaaaa
     4501 gccgtaatca tcaattctcc atcgaatccg actggaatga tttatagcaa gaatgaactg
     4561 caggcaatcg gtgaatgggc agtaaaaaat gatattttga tagttgctga tgatatttat
     4621 ggccgtttag tttataatgg caatgagttt actccaattg caacgatttc agaagcaatc
     4681 aaaaatcaga ccatcatcat caacggcgtg tctaaaacat atgcgatgac aggctggcgc
     4741 attggttatg cggtgggaaa tcctgtgatc atcaatggaa tgatcgctgt tgcttcccaa
     4801 tcaactagca atccgacagc agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag
     4861 caagatacag tggaagaaat gcggaacgcc tttgaagaac gcctgaatac gttatatccg
     4921 cttgtggcag aactgccggg tgtttctctt gaaaagccac aaggagcttt ttatcttttt
     4981 cctaatgtaa aagaaacgct acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa
     5041 gatcttttag aagaaacagg ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa
     5101 aatgtccgca tgagttatgc cactgatcgc gctacgttag aagaagcggt acgcagaatc
     5161 aagcaattta ttgagagtaa aagtcaaaat taatcctgaa gaaattcaag gaatataaaa
     5221 ggagagtcat acgtggaaca aattcaaatc atcgattcga aaaaccatgt gggagaaaca
     5281 gtaacaatcg gcgcttgggt cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa
     5341 ttaagagacg gaacagcctt tttccaaggt gtagttgtaa aaagcgaagt gccggaagaa
     5401 gttttccaaa cagctaaaaa tctgcaccaa gaaacatccg tgttagtaac tggagaaatc
     5461 cgtgaagaca gccgttcaaa atttggttat gaaatcggaa ttacaaatgt ggaagtggta
     5521 agcgagagcc acgattatcc gatcacacca aaagaacacg gaacagactt cttgatggat
     5581 catcgtcatt tatggctacg ttcttcaaga caacatgcga ttatgcagat cagaaatgaa
     5641 atcattcgtg caacgtatga attctttaat caacatcatt ttgtcaaaat cgatccgccg
     5701 atcttgactg gttccgcacc agaaggcact acggatctat tcgaaacaaa ttactttgat
     5761 caaaaagctt atttatctca aagtggccaa ttatacatgg aagctgctgc tatggctttc
     5821 ggaaaagtct tttcattcgg tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat
     5881 ttgattgaat tctggatgat cgaaccagaa atggctttca ctcatcaaga agaaagttta
     5941 gaagtacagg aacaatatgt agcattctta gtacaaaatg ttttagatca ttgcgaatac
     6001 gcattagatg tactaggacg agacaaagaa gtcttaaaaa cttatacaaa attgccattc
     6061 cctcgtatta cttatgatga tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc
     6121 gaatggggcg aagactttgg ttcacctcat gaaacgttca ttgcgaattc attcgaacag
     6181 cctgtcttta tcctaaacta tccaaaagct atcaagccat tctacatgaa accacatcca
     6241 acaagagaag acgtggtaat ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt
     6301 attggcggaa gcgaacgggc aacggactat gatttcttgc tagaacagat ccgtaatcac
     6361 ggtttggatg aaaaagaata ttcatggtat cttgatctac gtaaatacgg ttctgttccg
     6421 cactctggtt ttggtctagg ccttgaacga acagtcactt ggattgctgg tatcgaccat
     6481 gtgcgtgaag ctattccatt cccacgtttg ttgaaccgaa tttatccata agaggtgatg
     6541 aaaaagctgt tctgcatcaa gtaattagaa cagccaagca aaaacagctg ttcatgtttt
     6601 tgaattggcg ggacttatta ccgcagatgt aagcttttga ataccgttta gtggagaggt
     6661 tgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtggaaatg gctttccaca
     6721 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttaaggaaga
     6781 ggttgtgaaa tcagtgcctt gacctgagta ttagagaaac aaaacggaaa aatagcttct
     6841 catattttac cgttttttta gctaatatcg agggtcagct gattgaacac cgtttatatc
     6901 aagaggttgt gcgcctgccg ttcagctccg agtcacaagg agtaatttca aaaaacagtt
     6961 tttcatgttt gatgactatt gtgacttgtg gccggagagc aacgtttatt taaaaaaaag
     7021 aactgcgaac tctcgcagtt ctttttttaa aatacgggat aatggaagtt tccagcagac
     7081 aagagaagaa agcaaaagaa attgctattt gattcacaag gttctataac gaaatcgcaa
     7141 tcaatcaaac atttacaaag aaggtaattc aaatggaaat ctgatttgat gatgcttctg
     7201 ctgttgaaat aaagcagcat ctagcacctg aaaacaaact aatagtaaca ttcgaagatg
     7261 gagtaggtcc gtattttcag catgcaataa tccatatgta agttcaattc acccttgaaa
     7321 tcatcggacc agataaagtt atgaccaatc tatcccaagc aatctagaag agatattagt
     7381 aaagggacat ttactagaag tcttagatga gcctataaat atatgttttg atggcgtcat
     7441 cagtggtctg attgacggaa gcttgggtat gatcgatctc tctggaccaa acaacgaagg
     7501 aatcaagggg aatccggcac gttaagaaaa aaatagcata aaaaactaga cacttgatga
     7561 gcgttcattc aattgtttga agggactagg ctggtgtcat tttttataca tatattttct
     7621 ctttcatctt gatagggctt atgggataat ataaggaaga aaatcatcgt tttattcaaa
     7681 aaggagtttt tgacaaatga aaaaacgtat attatttatc ggtgatagca ttacagacgc
     7741 aggaagagac cgagcagaag caactgatct aggtaaaggt tatccacttt tgattgccgg
     7801 aaaactaatc tcccaatatc caacgatgga atttgaatgc gtgaaccgtg ggataggagg
     7861 agacaaaatt gcagatctgc aagtacgctg gcaagaagat tgtttagctt tacaacctga
     7921 ctatgtatcg attttgatcg gaatcaatga tacctggcat aatactagcg atcaggctat
     7981 attcggaact gagaaatctg ctcagcaatt tgaatccgtt tatcgaaaat tactaaatga
     8041 acttcaaaaa gcgggaatct gcaacatcat cttgatggag ccgtttgtac ttcctataac
     8101 cgacgaccgt catggctggc gtaaagactt agaccctaaa atccaaattg ttcgtaaact
     8161 tgctaaggaa tatggcgcat tgctggttcc tttagatggt atattcaatg ctttagggat
     8221 tgcttatggt ttttcgaaat atacaagaga agatggggtc cacccaacga ttggcggaca
     8281 tgagatcatt gcacaaagct ggatagatgc aattgccgca gtcgattttt ttagtggaga
     8341 taaaaatgag cgaaagaaaa gtgaaaaatg atggaaataa ggtgggcagt aaaagaagat
     8401 ttacctcaaa tcaatcagct gattactgat catccagaat cattcgtaaa agcaaaaacg
     8461 gcagctgacc taaatgataa gaataaacta ttgctcattg ctgtaacaaa agaaaaacag
     8521 attatcggat ttgcgcaagc ggacgtgcaa cagtcagaag aacgattgga tcagcgacca
     8581 tcaggaaaac taatccaaca cacattagta acaaaagaaa atacgaaaga aatccaaaaa
     8641 aaatttgcag aatacttgat gctggaagct aaaaaaagaa gaataaaatc aatttttgcc
     8701 cagttttaat caaaaacagt aatagaagga gaaatacaat gagttgttta ggaaatgtca
     8761 tctggtttat attcggcgga ctaatcggtg gtcttagctg gttagcagcg gggatacttt
     8821 ggtgtatcac gatcataggt atcccaatcg gtatgcaatg ttttaagatc gcctcccttt
     8881 cttttgcgcc ttttggcaaa gaagtaattt atcaccagtc tggcatgtca ttgatcgtta
     8941 atatcatctg gctgatcgtt tcaggcctac caattgctct cgcacatgtc gtcagcggca
     9001 tccttctatg tatcacgatc atagggatac cttttgccaa gcaatctttc aaacttgctc
     9061 gattggcgtt gatgccattc ggtgcagaaa ttgtataaaa gagaataata aaggttagat
     9121 aacgcataga gtcaggtggg atacatttat ttcacctgac tctatgcgtt ttcctgtata
     9181 agttcttcca tgtgactttt acgtctttta tttatatggg attactgttt atggaaagtt
     9241 gaaataattt tcattaaaga actctttcta tatttttgaa agcgtagtgg agcttcgaat
     9301 atctgggaag gacattttgc agggattgaa aaaagatatt gtcctatcga aaaattaatg
     9361 aatttagaca aattgccagc aacaggcttc aaacttagtt gctatcctgt caagatcaag
     9421 aaggcaagtg cgggttggat aagagcagtg gcaatgatcg aaaagaaaaa gaaagaataa
     9481 gccaaaacat ctgtatggaa gaaattttcc gatgcagatg tttttcattt tttctttcgg
     9541 ataccttgac atatgaataa ccattcattt ataattagtt atgaagagat attcatatgt
     9601 tgaggcgagg aggtcattaa aatgaaaagt tacagattag aaggtttaga ttgtgcgaac
     9661 tgtgcaatga aaatcgaaaa aggagttcag aaaatcaatg gagtcaagga agcaacggta
     9721 aacttcactt ctggaaaact aacgattgac gcagaggaag atcatttggc tacgattgaa
     9781 caagaaacaa aaaaagtagt gaaggaatta gaaccagatg tcaaagtaac agaaatagat
     9841 aaggaaaaag tatctgaaca tggaaatgaa aaagagagaa atacgctgtt ccggatttta
     9901 ttttcccttg caggtatcgc tttattgcta ttatttgatt ttaatgaacc aattcgcttg
     9961 atagggtatt tattgattta tctgctgata ggatatgatg tagtgaagaa ggcagtgatg
    10021 aatatcgtca aaggaaaaat ctttgatgag aattttctta tgtctgttgc tacgattggc
    10081 gctatgttga tcggtgaata tccagaagcc gtagcagtga tgctatttta tcaaatcggc
    10141 gagtatttcc aaggattggc agtcagtcat tcaagaaaat cgattcgcga attgatggcg
    10201 atccgtccag aaacagctca cgtgcagacg gcggaaggat tgatgaccgt gaaccctgaa
    10261 gacgtgcaga tcggtcagtt tgtacttgtg aagcctggtg aacgtgtacc gttagatgga
    10321 acgatcatcg aaggagaatc attagtcgat acgtcagcac ttacaggaga atcagtccct
    10381 aaatctgtat atgttggtga aacagtatta agcggattta tcaataaaaa taaaccattt
    10441 ttagttcaag tagaaaaatc ttatgaaaat tctactatca gcaaattatt agaattagta
    10501 gaaaatgcca gcagtaaaaa agcaccagct gaaaatttca ttactaaatt cgcgcgctat
    10561 tacacaccag tcgttgtagg attggccgtt ttattagctg ttttacctcc acttgttgtt
    10621 tctggagcgg cgttttctga atggatctat cgagcattga catttttagt gatctcatgt
    10681 ccatgtgcat tagtgatttc cgtgccactt agtttctttg gcggtatcgg aggagcaagt
    10741 aaaatcggtg tattagtaaa aggttcaaat tacttggagt tgcttgcaca aactgagaca
    10801 gtggtattcg ataaaaccgg cacattaacg aaaggtgact tttcgatcca gacgatcgat
    10861 acaaaaatcg atccaaagat attcatgcaa tatgttgcga gtgcagaaca gttttctaca
    10921 catccgattg ctcaatctgt tttagaaggc tacgcaggtc cgcttttacc aacaactaat
    10981 atccaagaat ttgcaggtga agggatctta gctgaagttg atggcaaaca agtattagta
    11041 ggtaatcaca aattgatgga aagatttgaa atctcttttc cttcaagtca agaaatcggt
    11101 acattgcttt atctagcgat cgatcaaagt tacagtggtt atcttgttat tgcagatacc
    11161 ttgaaagaag atgccgtaga cgcattggtt caattaaaac aggctggtgt gaagaacaca
    11221 gtcatgttga cgggcgactc gaaaaaaatt gctgatcata tcgggaagca agtaggcgtg
    11281 gacaagatat atagcgaatt gcttccagaa gataaagtgc agcggttaga ggaaatctta
    11341 caaagaaaca ataaaaagac cgcatttgtc ggagatggaa tcaacgatgc accggtgcta
    11401 gcaagagcag atgtaggaat cgcaatgggt ggattgggaa gcgatgctgc aattgaagca
    11461 gcagatgtag tcatcatgaa tgatcaacca tcgaagattg cagaagcaat ccatttagca
    11521 aagaaaacat tgaaaatcgt caaacagaac attgtctttg ctattgggat aaaaattttg
    11581 gtacttgcct taggtgcttt tggttttgca tcaatgggag atgcagtatt tgcagatgtt
    11641 ggggtgacag tattagctgt tttgaatgcc atgcgaagct tgcatgtaaa aaataaacgt
    11701 tgatcaatga agaggacgaa tgaacgtcct ctttttttta tatccttttc ttaatgaaaa
    11761 atgtaaa
//
LOCUS       DACCWK010000077        11607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCWK010000077 DACCWK010000000
VERSION     DACCWK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.079"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(119..1249)
                     /locus_tag="IVW65_001978"
     CDS             complement(119..1249)
                     /locus_tag="IVW65_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7900850.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1790..1996)
                     /locus_tag="IVW65_001979"
     CDS             complement(1790..1996)
                     /locus_tag="IVW65_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900851.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2012..2626)
                     /locus_tag="IVW65_001980"
     CDS             complement(2012..2626)
                     /locus_tag="IVW65_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7900852.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2773..3456)
                     /locus_tag="IVW65_001981"
     CDS             complement(2773..3456)
                     /locus_tag="IVW65_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7900853.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3565..4023)
                     /locus_tag="IVW65_001982"
     CDS             complement(3565..4023)
                     /locus_tag="IVW65_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7900854.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4107..5870)
                     /locus_tag="IVW65_001983"
     CDS             complement(4107..5870)
                     /locus_tag="IVW65_001983"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7900855.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6091..7551)
                     /locus_tag="IVW65_001984"
     CDS             complement(6091..7551)
                     /locus_tag="IVW65_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900856.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIKRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="IVW65_001985"
     CDS             complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="IVW65_001985"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7900857.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8708..10135)
                     /locus_tag="IVW65_001986"
     CDS             complement(8708..10135)
                     /locus_tag="IVW65_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7900858.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10166..11380)
                     /locus_tag="IVW65_001987"
     CDS             complement(10166..11380)
                     /locus_tag="IVW65_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7900859.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3511 a         2403 c         1913 g         3780 t
ORIGIN      
        1 taatatgatt ttttgagata ctttcattgc gaattcctct ttctaaaaga acctctcgct
       61 tgaaaaataa aagattgtac tttatgataa ttcaaactga gagtttctca gtttctcttt
      121 attgtttttc aggtcgatag agcgccaact cttcggcctg tatctttgaa gaaatattca
      181 acgtgattcc gattcctaaa gaaaggatca gataacttgt tcctccgtag ctaacaaatg
      241 gcaatggcac accagtcatt ggcatcagcc ccgcgatact tcccacgttc attatcgtct
      301 gaacgaatag caaagttccg aatcctaaaa gaaacaatcc agcttgctga tttttacagc
      361 gactgcttaa acaaaagatt ctcatgcata atgagaacaa taagaaaagc acacacaatg
      421 caccgattag cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt tctgtctctg
      481 gaagataccc tttttttgtg atactatttc ctaatccccg cccaaatatg ccaccattat
      541 atagtgcata aaacgaatgg gtcatttgat agcccgcacc atgactatcg ataaaagaat
      601 ctcttaaagt cgcgatcctt tcataagcat gattaaacat ctgtggaagc caacctttat
      661 ttcctaaata taataccccg cctgctgctc caatcagcaa tgcagaaaaa gtaacgatta
      721 gatagatccc ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac gcaatcgaaa
      781 ggatcatcaa tgctccagca atctttggct gaaataaaat cagaaaagtt atacctactg
      841 taatcaggaa tggttttttt agatcttttg ggctactatt tccgtcacga aaaaaccagc
      901 ttaaataaaa aatcaaaaaa agatttgcca gttcagaagg ctggaattga atgccaaaca
      961 gagagatcca acgttgtgcg ccgttgacag tgacaccgaa tatccctatt cttactaata
     1021 ctaaaaagaa aatcgataag gctaaaccgt atcctgctat tttagggtga agtaaatagt
     1081 gtagtttgac tgaacgagct aagaagatca cgctccaact caaaaatatg aaaattagtt
     1141 gacgcaataa tagacttttt gtattttcat cggcttgcaa taaacgataa gaactggcac
     1201 tgtatacttc taaaagaccg atcatggaca aagtcaaata aggacccaaa atccaccaat
     1261 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     1321 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     1381 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     1441 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     1501 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     1561 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     1621 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     1681 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     1741 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     1801 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     1861 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     1921 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     1981 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     2041 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     2101 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     2161 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     2221 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     2281 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     2341 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     2401 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     2461 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     2521 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     2581 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     2641 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     2701 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     2761 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     2821 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     2881 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     2941 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     3001 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     3061 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     3121 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     3181 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     3241 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     3301 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     3361 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     3421 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     3481 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     3541 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     3601 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     3661 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     3721 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     3781 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     3841 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     3901 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     3961 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     4021 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     4081 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     4141 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     4201 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     4261 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     4321 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     4381 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     4441 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     4501 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     4561 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     4621 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     4681 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     4741 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     4801 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     4861 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     4921 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     4981 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     5041 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     5101 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     5161 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     5221 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     5281 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     5341 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     5401 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     5461 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     5521 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     5581 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     5641 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     5701 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     5761 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     5821 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     5881 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     5941 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     6001 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     6061 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
     6121 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
     6181 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
     6241 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
     6301 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
     6361 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
     6421 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
     6481 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
     6541 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
     6601 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
     6661 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
     6721 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
     6781 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
     6841 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
     6901 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtttgat
     6961 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
     7021 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
     7081 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
     7141 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
     7201 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
     7261 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
     7321 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
     7381 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
     7441 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
     7501 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
     7561 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
     7621 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
     7681 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
     7741 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
     7801 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
     7861 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
     7921 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
     7981 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
     8041 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
     8101 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
     8161 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
     8221 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
     8281 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
     8341 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
     8401 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
     8461 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
     8521 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
     8581 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
     8641 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
     8701 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
     8761 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
     8821 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
     8881 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
     8941 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
     9001 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
     9061 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
     9121 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
     9181 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
     9241 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
     9301 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
     9361 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
     9421 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
     9481 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
     9541 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
     9601 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
     9661 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
     9721 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
     9781 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
     9841 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
     9901 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
     9961 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    10021 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    10081 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    10141 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    10201 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    10261 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    10321 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    10381 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    10441 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    10501 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    10561 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    10621 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    10681 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    10741 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    10801 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    10861 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    10921 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    10981 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    11041 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    11101 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    11161 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    11221 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    11281 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    11341 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    11401 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact
    11461 tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
    11521 tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg tttattttcg
    11581 tcttttttta tttttaaaga tattttt
//
LOCUS       DACCWK010000078        11530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCWK010000078 DACCWK010000000
VERSION     DACCWK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.080"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            343..549
                     /locus_tag="IVW65_001988"
     CDS             343..549
                     /locus_tag="IVW65_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7900860.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(751..1752)
                     /locus_tag="IVW65_001989"
     CDS             complement(751..1752)
                     /locus_tag="IVW65_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7900861.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1757..3670)
                     /locus_tag="IVW65_001990"
     CDS             complement(1757..3670)
                     /locus_tag="IVW65_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7900862.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3838..4344)
                     /locus_tag="IVW65_001991"
     CDS             complement(3838..4344)
                     /locus_tag="IVW65_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7900863.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4504..4944
                     /locus_tag="IVW65_001992"
     CDS             4504..4944
                     /locus_tag="IVW65_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7900864.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4970..6127)
                     /locus_tag="IVW65_001993"
     CDS             complement(4970..6127)
                     /locus_tag="IVW65_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900865.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6130..6486)
                     /locus_tag="IVW65_001994"
     CDS             complement(6130..6486)
                     /locus_tag="IVW65_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900866.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6784..7758)
                     /gene="sgrA"
                     /locus_tag="IVW65_001995"
     CDS             complement(6784..7758)
                     /gene="sgrA"
                     /locus_tag="IVW65_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7900867.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7954..8793)
                     /locus_tag="IVW65_001996"
     CDS             complement(7954..8793)
                     /locus_tag="IVW65_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7900868.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8978..9865
                     /locus_tag="IVW65_001997"
     CDS             8978..9865
                     /locus_tag="IVW65_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7900869.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10449..11144)
                     /locus_tag="IVW65_001998"
     CDS             complement(10449..11144)
                     /locus_tag="IVW65_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7900870.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11128..11526)
                     /locus_tag="IVW65_001999"
     CDS             complement(11128..11526)
                     /locus_tag="IVW65_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7900871.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3431 a         2301 c         1729 g         4069 t
ORIGIN      
        1 ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg atatgattgt
       61 tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt ttgggggtat
      121 cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg tatgatggta
      181 aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca tctttgtttc
      241 aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt tatagattct
      301 taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc tatccatatt
      361 tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg ttcccagcct
      421 tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc actcggcttt
      481 ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg atcaaaacaa
      541 caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg tgatattccc
      601 aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa gttacaatat
      661 tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga gctaagtgac
      721 agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta ctaacttttg
      781 atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt tcaaaacagc
      841 atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa atacagaagt
      901 tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga atgtgttttg
      961 cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg attgttcaaa
     1021 gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag agattttatc
     1081 acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa acttcgtaat
     1141 ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt cattttgaag
     1201 tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa tcgaaatatt
     1261 cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt cgttcaattg
     1321 gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat ttacgatgat
     1381 ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt tatcccctcc
     1441 tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt ccactttcgg
     1501 tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt ctttttttat
     1561 atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt gttctttttc
     1621 acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga tttgattaat
     1681 caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca ctattcgctt
     1741 gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata atcgtgctaa
     1801 cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca gttttcggca
     1861 acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga atcggttgcg
     1921 tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct gcattctctg
     1981 tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa ataatagttg
     2041 gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat tcgtatcctt
     2101 cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc tggatttcgt
     2161 cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt atctgatcat
     2221 ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg tcaacttcct
     2281 gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct cctgataact
     2341 catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca tggataatca
     2401 atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta tccaaatgca
     2461 tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca tgctcttgat
     2521 ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct actgtctggc
     2581 tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca ataattcctt
     2641 tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct cccatcgaac
     2701 caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt atccagtaaa
     2761 ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta ttaccagtcg
     2821 ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg tattcttctg
     2881 taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact gttttatctg
     2941 aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt ttgtttagta
     3001 cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta ggcgttgtcc
     3061 catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa gcgatatcgt
     3121 acaatagata agtagcaatc agatgtttga taatctcttt atctacacca taatcattaa
     3181 cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct gttggtccat
     3241 taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca ttgaaatttt
     3301 cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact ttagcagaag
     3361 aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg ttatctgtgg
     3421 tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt tcagtatttg
     3481 tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt tctgtggttt
     3541 ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc cattgcggga
     3601 cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt tttgttctca
     3661 agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa cattttttat
     3721 acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc attttacgta
     3781 ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag aaacaactta
     3841 ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag cgattgtttc
     3901 ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca tcataagctg
     3961 gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg cttgtgcccc
     4021 acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat tagtcttata
     4081 aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag ataattccga
     4141 caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt gaacgaaaat
     4201 aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg ctgtcactgt
     4261 ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat cgatcactac
     4321 tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg atttaatagt
     4381 atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta cacctctaaa
     4441 tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct attggaggaa
     4501 tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg cgcaaaacgt
     4561 tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca accactaacc
     4621 aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga aatgctctct
     4681 actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag tttgagtgaa
     4741 ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat ctacgaccaa
     4801 tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct ttctaaaact
     4861 aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga ttttaaagaa
     4921 aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttct taattaacga
     4981 aatccaattt ttccaatatg gatctatttt tctcattcct gatttttatg atttcagctt
     5041 tcagacgcgc ccaatcattt tttgtaggtt gtccattcca gtaaaatatc tgtgtattcg
     5101 tttgatcata tgcctttttg tcgataggat aatcgctaat gacaatatca ggtaactgat
     5161 cagcggatgc actatatttt attgggcgtt cagtcataga taatatcctt ttctttgccc
     5221 ataattcctg tgttttacca tactttgatt gaatatagaa tatgacaggt tgttcgtgtt
     5281 ttataaaaat atccctgact aacatacagt attgaaaaat caaccgtctg ttcttttcat
     5341 aataagtatt aagctctgga attctgcgag ctaccccact aaaaaactgt tgcatgattg
     5401 gatacatcac tggaaatata tgctgaaaat ctctgtcttc tgctgatttt ccaaaagcat
     5461 ccaccttctt ttttgtccca aaaacccttg aatacaaatg gatcattgcc aaattgacat
     5521 atagataatt tttttctgaa gttgtaaaac aaatcttgaa gtttttttca aaattttcta
     5581 caatagacga ttcaatccag ccccaacttt cttgtacttt atctggagta tccaacaaat
     5641 cttcttccaa ataggtgttc attacaccaa acataaaata aaggtaagct tgctctgttg
     5701 gagtgatgtg attcatcgac cagaagtttg cttcaaaaaa aggctctagt atgtaatgcg
     5761 taaactcttc ttttgttatc aatggatgca caaatttgtt catctcttct gggacatacg
     5821 taatcgagtg gccttgtttc attcgttgca acgaaattct tagtaggctg atccatttct
     5881 ttctatctat actacgcgta tatggcgcat gtttcgcaaa tttttctaat agttgacgtt
     5941 tattttttat acttgataat atatcagaat gattttcgtc gaatgactgc caatacatca
     6001 agtgaaaaaa gtatcggata cggtattctt ctccttctat cggattacct tttttcaagc
     6061 tgcaagataa atgatagttt ttcaaaatag tattaagttc tcttaatttt ctttgaatcg
     6121 tacgcaaact tatataattt tttgactaag attttctaag gattgataag tacctttgta
     6181 aagtgaatcc agtaaaatat aaccaatcgt tccctctaaa agtatagaat aaactttact
     6241 cagtaaaaac tgagaagaaa actcgacttt tatcatcttg ttttctgaaa gaaaagataa
     6301 tttatattct ggataccttt cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt
     6361 agccagggtg tagtttagat ctaaagcgat atccttcacc acatataatt tctcgtctct
     6421 ttcataacaa tagttcaaaa attgatatac ttgcctttca ttgatatcaa taaattcttc
     6481 aaacaaagta catactcctt tttatctatt atttagactt attccgacag ctacatgaat
     6541 aacgtacaaa tagattcgtt actgattttt cagagatact accatgcgca gtgtgcattg
     6601 tcaattattc ttgctcttat tttccatttg tgttcctgtt atttcctgtt tctcaaatat
     6661 gttagtaaac aaaaatagat ctggcaaagc tacaacttcg ccaggcaata cagcactctc
     6721 gaatgaaata ttcactcaaa aatctatcgt tttattcaat tttagatctg ttgcttttat
     6781 tctttatttc actcgatgga agagagaacg aaaatagctt gctcctacaa tcaaaatgag
     6841 taacacaccc atccatgtaa ttagcttgtt caaggttcta ctaccagttt ctggtaaatc
     6901 gctttttcta ttatctgttt ctttcgcttt ttcttgatga tcaattccac gagaaacaaa
     6961 atcaggctgt tttagcacac tttttaggtt gctccctgca tttctctttt gttccttagt
     7021 tggtatgata ggtaccaaat caagttcatt atttactttt cctgtgctgc tttcgctgct
     7081 cgtgcttcct gtgctactct cactactcat gcttcctgtg ctactttcac tactcgtact
     7141 ctccgtactg ctttcgctgc tcgtgcttcc tgtgctactt tcactactcg tactctccgt
     7201 actgctttcg ctgctcgtgt tttctgtgct actttcactg ctcgtactct ctgtactgct
     7261 ttcaccgctc gtgttttctg tactgctttc actacttgtg ctatccgtac tgcttggcgg
     7321 tccaacaact gtccaactgc ttggtttcaa attctccgtg atcgtcatct gtccatcata
     7381 aaaaacaatc aaatcaactt tatctttact cttgatctca tcaatcgata gttctgcaga
     7441 aatcattatt gtatactcaa taccagcggt ccattgtacg tcttttaaat aaacttcccc
     7501 aataactttt tctgaacttc cattaccatc ccaaccatat gttagaacag catgtacatc
     7561 actagcattc acgcttttgt ttggtttgac tggaatagta atgacttgtt gtgtcatgtc
     7621 ataaacaatc cgatcagttt tcacagtaaa tgaaagattc tcatttgccc gttcatttgc
     7681 agaagtactg attccaatca ttgctgcaat tatactcagc ccaagaataa caaataatac
     7741 ggtcgctgtt ttcttcattt ctatcactct ccatataatt accttctatt tgataaagga
     7801 attatataaa atgaaaatga tgagaacacg acagtcagtt gtgaaaatct aacaaaagat
     7861 gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat caatgacaga cttaaaaaag
     7921 caagaccgat atcagccttg ctttcttcat cttttattga taaacagcag tgtatttctt
     7981 catccgcttt gttttatatg catagagtaa caaaccaaca atcaagacta acaagaataa
     8041 tgtagttgat atagatgctt cagccccaaa gtttccccca gtaattattg tattttgcga
     8101 ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg ctgactggta tacccaaaat
     8161 agctccttgt gtccaattcc aagtaatatg agcaccaata gccgtaaata aacttcctgt
     8221 actgtagata agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa
     8281 tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat gcagcagatc caatcattgc
     8341 tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata aaagctcttc
     8401 tgatgccccc actgcaataa ataaaataaa actcgttatc agcggcacaa tgtttgcgtt
     8461 tcctgaatct aaccgatacg cccccgtcaa atataatgga ctagtaataa gcagaaacac
     8521 tccaattcca acaacgagac ctcccgctaa atacccccag tcttttcctc gaacagaaac
     8581 accataatct tctattcttt ttccttcgac cttactagca agaaataaag ctagtaaaaa
     8641 aacagctaaa tgtccgaccg taccggcaat tgctccaatc cattcactat ttccaataaa
     8701 catatccgca agtgcattat tgacaagaat tcctaaaaca aaaccaacta agactattaa
     8761 aacagatcca atcaaccgaa taatcctgat catctttcat ccctccttta tattaagttt
     8821 aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa tgttctccat catagagaga
     8881 atagattttt acgaactaag aatatctaca gcaaccttac gaaaatgaaa gactgtctcc
     8941 ctaattcttc tgttagactg tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca
     9001 actactaatt atgtgccttt cagcatgtgc aattttacta ttctcaggtt gtagcaagtc
     9061 caatgaaacg attgcaacgt ttaaaggtgg aacacttaca gttcaggatt tttatgataa
     9121 cccaaaagtt aaatcaaata atcaaacact attaaaaaaa atgattgtct tcaaagcatt
     9181 tgatgatgta tacggaaaag agatttcttc aaaggaagta acaaaagaat ataatgaaca
     9241 aatcaaaaaa ctcggtccaa actacaagga acaattaaaa gcagttggcc aaacagaaga
     9301 gacatataag aaactcttta aacagatgct ggctttccaa tacggtttga aagccaatgt
     9361 aaaattaaca gataaagatc tagacactgc ttggaaagaa ttctatccag aagttagtac
     9421 acagatcatt ctattttcga ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga
     9481 aggggaaaat ttctccaagc ttgttcaagc ttatggaaaa aataaaacat taaaggaaac
     9541 tgatggaaaa atgaattttg attcaaccaa tccagagatt cctactgaag taaaaaaagc
     9601 agcatttaaa ctaaaaaacg gggaagtttc tgatatcatt cctgtaacag atccaacgac
     9661 ttatcagcaa agctattatc tagtaaaaat ggtaaaaaaa caggataaag gttctaataa
     9721 agacaaatac aaatctgagc tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga
     9781 attcatggat aaaacaatcc gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc
     9841 ttatgtaaaa aatatattca aataaacaaa tttgacccaa ttggggatgt ggcaaaattt
     9901 gagaagtgat ttttggaaat tccttcttat ttttgaggca aaccactttt gccacaactt
     9961 catcggggcc tttttttatt tgtaccaaaa caaatcactg gatttgttac aatcatgagg
    10021 atataattta aaaaagtttt ttatttctat cacatgaatc aatgcgccat taagtagatt
    10081 ctttcatgct tccctatttt ttcacttgcc gtatcgcttc aaaaacaact atcataaact
    10141 caaactcgac tgaaaagtta ttttaaaaaa gactaaaaat atcccttctg gacttgttcc
    10201 tcttattctg ttatactaga gaaacacgaa agatattttt atgcgacact tttcatttgt
    10261 ttttatgaaa tttattcaaa actggcgtaa cttcagagtt acaaggatgt attagaggtt
    10321 agggaataca tcgtttaaat gagaaataag taatagtcac ccaaacttta aattaatgaa
    10381 tattgaatcg tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag
    10441 aaattgattc agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa
    10501 gtttgcaccc aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat
    10561 acttgatcta gtagctcttt acctaacgtt atcgcatgag ctccagatcg aatggctgta
    10621 gtgacttgtt cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt
    10681 tcgatctcag tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc
    10741 ctgttataat aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat
    10801 tctgtgtaaa tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct
    10861 tgaaggcctg cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg
    10921 ataactcgag catcctctat catttcttct tccgttcttc ctattgtctg aatatggata
    10981 ggaatctgtc cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct
    11041 tcttttttca gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa
    11101 gaataaatac tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt
    11161 ctgatcctct tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc
    11221 ttctacatac attgttcctg gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat
    11281 atgtcctaat tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat
    11341 ttgtttatca aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc
    11401 aatcttataa caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat
    11461 tttttctttc ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat
    11521 cgtcatctct
//
LOCUS       DACCWK010000079        11445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCWK010000079 DACCWK010000000
VERSION     DACCWK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.081"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            185..1183
                     /locus_tag="IVW65_002000"
     CDS             185..1183
                     /locus_tag="IVW65_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7900872.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IVW65_002001"
     CDS             1295..1804
                     /locus_tag="IVW65_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7900873.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IVW65_002002"
     CDS             complement(1834..2493)
                     /locus_tag="IVW65_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7900874.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IVW65_002003"
     CDS             complement(2495..3259)
                     /locus_tag="IVW65_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7900875.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IVW65_002004"
     CDS             complement(3267..3944)
                     /locus_tag="IVW65_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7900876.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IVW65_002005"
     CDS             complement(3981..5366)
                     /locus_tag="IVW65_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7900877.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5335..5589)
                     /locus_tag="IVW65_002006"
     CDS             complement(5335..5589)
                     /locus_tag="IVW65_002006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900878.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IVW65_002007"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVW65_002007"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVW65_002008"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVW65_002008"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7900879.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IVW65_002009"
     CDS             6305..7162
                     /locus_tag="IVW65_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7900880.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IVW65_002010"
     CDS             complement(7243..7578)
                     /locus_tag="IVW65_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900881.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IVW65_002011"
     CDS             complement(7645..9015)
                     /locus_tag="IVW65_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7900882.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IVW65_002012"
     CDS             complement(9093..9629)
                     /locus_tag="IVW65_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7900883.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IVW65_002013"
     CDS             9938..11419
                     /locus_tag="IVW65_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7900884.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3517 a         2300 c         1855 g         3773 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatggtactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttatt
//
LOCUS       DACCWK010000080        11204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCWK010000080 DACCWK010000000
VERSION     DACCWK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.083"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(206..1054)
                     /locus_tag="IVW65_002014"
     CDS             complement(206..1054)
                     /locus_tag="IVW65_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7900885.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1218..2120
                     /gene="gnd"
                     /locus_tag="IVW65_002015"
     CDS             1218..2120
                     /gene="gnd"
                     /locus_tag="IVW65_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7900886.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2122..3123
                     /locus_tag="IVW65_002016"
     CDS             2122..3123
                     /locus_tag="IVW65_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7900887.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3120..3404
                     /locus_tag="IVW65_002017"
     CDS             3120..3404
                     /locus_tag="IVW65_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7900888.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3437..4234
                     /locus_tag="IVW65_002018"
     CDS             3437..4234
                     /locus_tag="IVW65_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7900889.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4248..5081
                     /locus_tag="IVW65_002019"
     CDS             4248..5081
                     /locus_tag="IVW65_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7900890.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5099..5956
                     /locus_tag="IVW65_002020"
     CDS             5099..5956
                     /locus_tag="IVW65_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7900891.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5978..6487
                     /locus_tag="IVW65_002021"
     CDS             5978..6487
                     /locus_tag="IVW65_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7900892.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6524..6925
                     /locus_tag="IVW65_002022"
     CDS             6524..6925
                     /locus_tag="IVW65_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900893.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7037..7390
                     /locus_tag="IVW65_002023"
     CDS             7037..7390
                     /locus_tag="IVW65_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900894.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7424..7867
                     /locus_tag="IVW65_002024"
     CDS             7424..7867
                     /locus_tag="IVW65_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7900895.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7884..8969
                     /gene="uxuA"
                     /locus_tag="IVW65_002025"
     CDS             7884..8969
                     /gene="uxuA"
                     /locus_tag="IVW65_002025"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7900896.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8962..9675
                     /locus_tag="IVW65_002026"
     CDS             8962..9675
                     /locus_tag="IVW65_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7900897.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10427..10627
                     /locus_tag="IVW65_002027"
     CDS             10427..10627
                     /locus_tag="IVW65_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7900898.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3748 a         1752 c         2415 g         3289 t
ORIGIN      
        1 ctactgtata ctaactttgt tatcgcatct aacaagtaac tcgaataata ccaatacaac
       61 cttctccaaa gatgcgatga catacgttct aacccaccga ctattgatgt tctgtaccac
      121 ccttgcagaa ccaaaaaagc cactaccaaa tatttggtag tggctttttt tgttttatgg
      181 atttttcgct ttttctgtta tggtgctatt ctttgatttc agaaatagta tgtcgaactt
      241 tagcgataga acgacgtgat tctttttcgt tgtaaaacat caaaatcaca tatagagagt
      301 ccaaaatgct tagttgagaa atacgggaag ctaaagcttc agatcggaat tctgtttctt
      361 cagaaataga aataaaaacg acatcgccaa gtttagcaag ggaagaatta gcttggctag
      421 taataacgat tgtttttgct ccagtttttc ttacggtttc agcaatatgg attgtttctt
      481 ttgattgccc tgtgtgagaa atcaaaacag cacagtcttt ttccgtcaat aaagaagctt
      541 ccatcaattg aatatggtaa tccgtgctgt gagaagttgc gataggggaa cgcaaaaatt
      601 tatgataagc gtcagttgct acaatctctg aaccgccaac accgaaaaaa tagacacgtt
      661 tagattcatt gataatagta gccgcttttt ttagatcctt ttgttgtaac aacttttttg
      721 tgtcatttaa agttgaaata ctagactcga acaccttttg cgccatagtg agctcagtat
      781 ctgttttctg aatattttcg tggatagaaa tagcagatag atcattttct tggatcaaca
      841 tggccatttt aaaatcttta aatccattat aaccaagttt ttttgtaaat tgaaaaaaag
      901 tagagtcagc aatgcccagt tcacttgcta aattgctaat aggactatga cttactttgc
      961 ttggattttc taaaatataa tcggcgatag cctgttcttt tgggctgaag tcgttgtaca
     1021 ttgttttgat ttgtagtctt atggagacgc tcatgagaat tcctcatttt cataataaga
     1081 ttatcatttt ttccgttctc attaatgtta aagatacaag gagagtagga aaaaatcaaa
     1141 caaaaaatat ttttgtaaaa tctatttaca aaaaaatttt tttgtataat gagggtataa
     1201 gaaatggagg cgctatcatg gaaattggtg taattggttt gggaaaaatg ggattgaatt
     1261 tagctttaaa cttaaaagag caccatcaca aagtacaagg actcgacatt tcaagtacag
     1321 ctgttagtca ggcaagagaa aatgacattc ctgcttatca agagatctct gaatttctag
     1381 ctacttttga gcagaaaaaa atcatctggt taatgcttcc tgctggagag ataaccgaaa
     1441 acatgttaag aaaacttttt ccaaaactcg gaccaggaga cattattatt gaaggcggaa
     1501 actcttatta taaagattca atccgtcgag cagaagagtt tcagaaaaat gaaatcggtt
     1561 acttggattg tgggacttct ggaggaatcg aaggagcacg acatcaagcc tgcttaatga
     1621 ttggaggaga taaagaaact tttctatccg tggaacaatt attcaaagat gtagcaattg
     1681 aaagaggata tctgtatgct ggtgcatccg gaagcggaca cttcttgaaa atgatccata
     1741 atggaatcga atatggaatg atgcaagcaa tcggtgaagg attccaactt gtacaaaaaa
     1801 gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg gaatcatggt tcggtgattc
     1861 gcggctggct gatggaaatc atggaagatc agttaaacga acatcccaat ttaagtaatt
     1921 atcggggaat cgtttctgca tcaggtgaag caaaatggac ggttgaaaca gcgctagaaa
     1981 tggctgttcc agttccagta attgcaacta gtttgttcat gaggaatctt tcacaggaag
     2041 aagatagttt ttcagcaaaa gtagtttcag cactaagaaa tgggtttggc ggacatgaaa
     2101 tagttgaaaa gggtgagtaa aatgccagat tatcatatag caatcgttaa ttccagcagt
     2161 ttcggaagag tctttccaca ccacttggaa agattaaagt cagttggaga cgttcatttc
     2221 tttactgttg atcaagagat tccaggaaaa gaactagcgg agttacttaa aggattcaat
     2281 atcattattg caagtgttac gccatttttt cgacgagatt tttttgaaaa caaagatgaa
     2341 ctgctattga tttcccgaca tggtattggt tacaacaata ttgatttaaa agcagcaaaa
     2401 gaacaccaga caatcgtatc tatcatacct gctttagttg aacgaaatgc tgtagcagaa
     2461 aataatataa ctaatttgtt gtccatgctt cgacgcacga cacaagcaca agcaagagtc
     2521 aaggacgatc gatgggaaga tcgggcggag ttcgtcggta ggacgctgtt taacaaaaca
     2581 gttggtgtga ttggtgcagg aaataccgga agcggagtaa tcgaaatttt gcgtaacggt
     2641 tttcgctgtg atgttctagc ttatgatcct tacaaatcag atttatacat cgaaagcttt
     2701 ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat cagatatcat ttgtttatgt
     2761 gcgaatttga cagaagagaa ctatcacatg atttctgata aagaagtcga tcagatgaaa
     2821 gaaaatgttt atctatctaa tagtgcaaga ggcgctctag ttgatgaaga agcgatgatt
     2881 aatggtctga aaagcggaaa gatcgcagga tatgccacag atgttttaga agaagaaccg
     2941 ggacgtcaag atcatccgta tttagcgttt gaacaagtag tgattacgcc acatatatcg
     3001 gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat gtgtcagaga tgtcgaacag
     3061 atcagtcagc accttcttcc tgaacgttct gtccagacaa agagcaacta tataaaagga
     3121 tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg ccctcaattt tatttatatg
     3181 aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc atacggcgcg aaaagagtac
     3241 tggtcaccca cggaacggta tcatgggaaa aggcgctacc taaattagta tttttgaatg
     3301 acgaaacgat tcaatttttt tatcatcgtt attctggaga atgtagttat gctgaagcca
     3361 gacgaatagc tacaatcatc aaaaaaatga aatcgatttc gtagttggcg ttggcggagg
     3421 aaaactgacc gatctagtgg gttatagtgc caatcttgca agaatcgatt tcggagtcat
     3481 ccctacttta gctagtaatt gtgctccatg gacacctctt gctgtcatgt ataaggagaa
     3541 tggacaagca gaagggaaaa cagaacactt ttacagacaa gctgcttttt ttataacaga
     3601 tccccaatta gtgattgatt cccctgttcc atatttcatt gccgggttag cagatacact
     3661 agcaaaatgg tatgaatctg agctcatctt agaacaagat tttttgcaag atgaatcatt
     3721 tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa gagattttga atcattctgc
     3781 taaagcaatc caagatatgg cacaaagaaa actgacatcg gagttcaaac agctttcaga
     3841 gattatcttt gctgttgctg gtttagttgg aggattagga gataaatacg caagaaatgt
     3901 ggcagcacat gctatgcatg acgctatcag taaatatatt ccggaaagcc atcgttttct
     3961 acatggagaa aaagtagcgt atgggatttt ctatcaactg gcactagaag gtcgctggga
     4021 aaaaatagac gagttatatc ctttgtatcg ggaattagaa ctgccggttt ctctagaaca
     4081 aatgggatta tttccgaaag ataaacagat aatagataaa atggctgaat ttattgattc
     4141 gaaagagaaa gtgcatttga ttcctataga ggtaacacag aaatcactga tacagtcaat
     4201 gatggaactg gaaggttata tcagaaagat ctgaaggagg aggacacatg gaaacattaa
     4261 gtgtagtaca aagtttgttg attgctttat gggtagccgc ggttatgtcc cgctggttag
     4321 gtggaggagc cacgttgacc ttacgttttt ctcctttgat gactggatta gtcgtcgggt
     4381 tgatcatggg ggaagttgca caagcgatga ttgttacagc cgctttgcaa atgatttata
     4441 tgggggtttt ttctccagga ggatcaatgc cagctgaacc atctattgca gcagctatcg
     4501 cagtaccagt cgcattatta ggggatctgt cgccagaagc agcgattgcc gttgcggtgc
     4561 cagtcggatt gctcggaagt tacttgtatc agtttcgttt tttcatcaat acttttttag
     4621 gtaaatatac agacaaagct gtagaagatt tgaatacaaa agctatggcc cgatcaatta
     4681 ttctttatcc aacggttgcc tcatttttat tattcgttcc gcttgttttc attgctttat
     4741 atttaggggc gccattgatc gctgatgtaa tcaaagcttt agagggaaca gtcgttatcc
     4801 acattcttga agtagtaggt ggagggttag cggcaatcgg tatcgcgaca actgtatacg
     4861 ttatcggtcg aaaagattat ctggtctttt tcttccttgc ttattttatg agtgttgttt
     4921 tgaaatcttt agatattacg atggttactt atgccatttt tgggattatt atcgctttga
     4981 tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc cacaagcaat aacaattcag
     5041 catcttttga tgacgaagat gactatgatg acggatttta atttaggagg aatgggaaat
     5101 ggctgaaata atggataatc aaacaggtgc tcctgaagaa atcacgaaaa aagatgtaac
     5161 aaaagcctat ctgagatggc attttgcaaa cgaaattcct cactcttttg aacgttactt
     5221 ggcgccatca ctactatacg gaatgatgcc aatcttgaaa aaactttata aagatgaaga
     5281 ctctttaaaa gcagcatata aacggcagtt actgttcttc aacactcaat taagctgggg
     5341 tggcggtgtg attactggcc taatgtcttc tatggaacag caaagagcag aagaagagta
     5401 tgaagaaaaa gagatcttga tgcaggatga cttgatgtat aacacgaaag cgggattgat
     5461 gggggcacta gcaggaatcg gagatgcaat cgattcagga accgtccaat atatttttat
     5521 cgcaatcgcg gtaccttggg cacaacaggg aagtccttta ggcgcacttt ttccttttat
     5581 ctgctttggg ttgtatcagg ttttactagg ggtatttttc gcaagacaag ctttcactat
     5641 gggccgtaat gctacaggcc taaagcaaaa cactggtgtt cagacagcga ttgaaatgct
     5701 atcagtttta gggttgttta tgatgggggt gcttgcggga aactatgtaa aagtagaatc
     5761 ttcattgaaa tttgcgatat ccggacgtga gtttgttatc caggaaattc ttgatcaaat
     5821 cgtgccaggg atattacctt tagctgtcgt aatgggtgtg tacttgttct atacgaaaaa
     5881 aggattgaaa gtcacacaag cattattatg gctgactgga atattaattg tgctagcagg
     5941 aatcggtatt ttataaaaag aagggaacgt gaataagatg ggtgtagtca atttagcgcg
     6001 tgtggatgag cgtttgatcc atgggcaagt aatggtaacg ttatctcaaa aaagtggagt
     6061 gaattccatt tttgttgtag acgaagttgt agcaaaagat aaatttatga gagatcttta
     6121 caaaaatgca ggaagccgta caggacaaaa aacaattgtc attacaccag agaaagctaa
     6181 attctattgg gatgaatatc aatttaaaga atacaattgt attttgatcg caaaaacagt
     6241 ccaaacgatt tacgacttag taaaacacgg aatccccatg gaagaattga atattggagg
     6301 gattgctcaa aaaaatcctg atacagatct tttagtgacg aaatctgttt atttaaataa
     6361 atcagatgct gaaaaattga aagaaatgaa tgaacaatat ggcgtaaaga atatttattt
     6421 tcaagccact ccatctgcac ctaaaacagg attaaaagat gtattgaaca agtttgattt
     6481 gtcataaaca aatgcttaga aaaaagataa agagggcgaa aaaatgaaga agaacgagct
     6541 gtttcgtgat tgggaatttc gttatcgcta tatttaccgt aagcgccgta caaaaaagag
     6601 taaacagcgt tttctttcag cattagtatc agatatttac agtatgagaa cagatgtgac
     6661 agtcattgct tacgatacgc tagcctaccg atcaaaaaat atttatgttg gagatatcga
     6721 aaaagcagag aaagttatct gtacttatta cgatacaccc gttcatgcat taggaagtta
     6781 tttcatgttt gattggaaag accaaaggaa aaaaacaatc tactctatat tattgtcctt
     6841 cattttgttg ttcagtttag gttggtgggg aatgatgata tataacaaaa atcctcatca
     6901 cgtattcgat ctgctatccg tataaacagg tatcactgta ctagcatttg ggagctactt
     6961 tttcttattg ggaaaagcag ctcgcggatg gcctagcaga caaactttta ttcgaaatac
     7021 ttcatccata ttaacgatgc tagaaatgat ccgtacgatt gatgatccga gtgtagcgta
     7081 tgcgtttgtt gatgaaggct gttatggaga aaagggctta gacagcgtga gatcgggtat
     7141 gaaaaaagag gctattcttt tttatctaga ctctgttggg gcagatacac cgctacagtt
     7201 ttcgggaaat tatttttcaa ataaagaaca gtggctgaaa caagttgata agttgaaaga
     7261 aaaaaacgtc aattatattt ttagtgcaag aaaaaagcaa gcgcagttct tctatttgac
     7321 caaaacagat ttacgaggga aaacatttaa ttggcaaaat gccaatcaga ttattgctct
     7381 ttttcgataa gtaaaaagtg caaaacaagg agtgagaaga ctaatgttgg aaatcatgat
     7441 cgttacacat ggtaagttga gcgaaggact gaaagatgcg gcggaagtaa ttgtcgggac
     7501 atcgagccat attgaaacaa tgtgcttaga gtctggggag gacatccaaa cattcggaga
     7561 gaaggtcaaa gaaaaattaa ctatgatcaa cacagcagaa cccatgctag tatttgttga
     7621 tctaattagc gcaagtccgt acaatcaagc gctcttggca gtcaatgaac tgactgaaga
     7681 aaagaaagca gccacttatg tcataggggg cgtgaatctg ccaatgttgt tagaagcaat
     7741 caatcatcag atgcttcaca caccgataga agaagcggtg gaacaaattg tcaaacaagg
     7801 aaaagataat ttaactgttt ggcatgcttc agcggaagaa aaaaaattag atgaggacga
     7861 tttttagaag gaaggctgaa aagatgaagt ggggatttag atggtatggt gaaaaagggg
     7921 attcgatccc attaaaatat gttcgccaaa taccgggaat aaatgggata attggaactt
     7981 tgctgactaa aatgcctgga gatgtttggg agatttcaga aattgaggag ttgaaagcat
     8041 ctgttgagag ccaaggactg gaactgcttg gaattgaaag tgtagcgatc cacgatgcaa
     8101 tcaaggcagg gacagatgaa cgagattact atattgataa ctatatacga acaatcaaaa
     8161 acttgtctcg atgtggggtt cgattgattt gttatagctt caaaccaatt tttggatggg
     8221 cgaaaactga tttgtttaat gaaaacaacg atggaagcta ttctctttta tacgatcaga
     8281 aaatcgtcga tcagatggag ccaggagaaa tgtatcaatt gatacacagc cagtcaaagg
     8341 gattcaaatt gccgggttgg gaagaagaac ggttgaaaaa attcgctcag ctggaaaaac
     8401 tgtatgaagg aatgaccgaa gaagaattat ttgaaaatct gacttatttt ttagaaaaag
     8461 tgattccagt gtgcgaaaaa gcagatgtaa aaatggctat ccatccagat gatccgccat
     8521 gggaactttt tggttttcct agagtgacga aaaatcttgg cgacttgaag aaaattctaa
     8581 atgcagtaga ttctccatat aatggactaa cgctgtgcac aggatcttta ggggcaaatc
     8641 cggaaaacga tttagttgaa atcatccatg aggtaggttc gcgcatccat tttgtccact
     8701 tcagaaacgt ggagttttta ggagaaagaa agttcaaaga aacggcacat ccctctacag
     8761 aaggctccct tgatatgtat catattatga aagcgttggt gcaagaaggg ttcgacggtc
     8821 ctatacgccc tgatcatggt agaaccgtgt ggggtgaagt ggctatgcct ggatatggcc
     8881 tgtatgatcg tgcaatggga ctcacttaca tgcaaggtct atatgaggcg ataagtaagg
     8941 aaaaaccact gggagagcat tatgaataaa atcaagctct tcatctttga tatggatggc
     9001 cttttattag aaactggacg tttagcttat cgtgcttatg taaaatcagc acaaaagtat
     9061 gattatgaaa tgcgtaagga agtctattat ttactaactg gccaaacaga aatggcgata
     9121 agagaacaaa tgggcatttt gtacggagaa gatgttccat atatcaaatg gcgagaagca
     9181 atcaatcaat ataaagaaaa aatcgtgaaa gaagataaga gagtatatac gaaaaaagga
     9241 gcagaagaaa ttttatcttt tgcaaaagaa agaggaatcc atacaatcgt ggcttcgtct
     9301 aatacacggg taaaagtaga aatgtattta aaaatggaaa atctatatgc cttgtttgat
     9361 caaatcattt caggggatga tgtaaaaaaa ggaaagccgg aaccagagat ctttttgaaa
     9421 gcttgttcaa aaatgaatat tcctccaagt gaagcacttg tatttgaaga ctcgattgca
     9481 gggatcgaag cagcaaggcg agcgggcatt ttgtcttttc tcatcaaaga tcatttgata
     9541 gggttgcccg atcataatgg gaagcataag ttgaaagtag atgtaagttt atatgaatcc
     9601 aataaagcag actatgaatt ttatgacctg catcaagcaa aacgattttt agaaagtaaa
     9661 gggttgtatc tctaataaag aggactattt tctagtcctc tttatttatt gtcctgcacg
     9721 actgagggaa cagatgaaaa agaagtaatt ttttattttg aagtatctac cttatttgta
     9781 gtgaacaatt tagaacaaac acaaaacgca tctactgtta tctaccagta gatgcgttta
     9841 aagagaagaa cgagaatcgt ccatatgagg gtttccactc cttatgcaga tttttttaat
     9901 tcttgcactg cttgttcgat cgtaacacct gtcgctacat gattggtatt ttctttgtca
     9961 aaaacgacaa atagattcaa atctgtatcc aaggttactc gatattgtat atctttgtta
    10021 tagaatgtca tagccattct cctttcttta aatacttgct gaaaggcaac gaacgcgttg
    10081 ataacgaaat tccatttata agtataccac caattgaaag gttttcatag ttaagaaatt
    10141 ttaaagtcaa aagttgcaga taaaagcaaa taaacaagaa aacaaaaagt gaatcaatcg
    10201 cggttattca actttttctt cgattcgaag acaatagaag acaatgaatt ttatatttca
    10261 ttgcattatc cttgtgttat tagaaaaagc atgatatatt aaatgagtaa tctattttga
    10321 aacgtaattt gagcgatata tctacataga taaaactcct tttgcctatc aaatatttat
    10381 tgcaacgaaa aaacgtacat tgatacgtat caggaggaat ttatttatga ataacggtac
    10441 agtaaaatgg ttcaacgcag acaaaggttt tggctttatc actggtgaag atggaaatga
    10501 tgtatttgca catttttcag ctatccaagg tgacggcttc aaaactttag aagaaggcca
    10561 agcagtgtct tacgatgttg aagatggtca acgtggccct caagcaacaa acattgttaa
    10621 agcttaataa aaattgactt ttaaccgtct tttcgaaaga aaagacggtt tttttgtacg
    10681 aaaaaattca ttggtcgttg aatgggctga atagtagtgt ataaaacaat tttgtggagc
    10741 gtgaagcttt tatggtagag gaaaatacga ataaagtgat gttgataaaa agattcctat
    10801 cttgaatatg aatcgagatg tcttgaaaat gaatgagttt gaccaatcat tatcctatcg
    10861 aaatttacag tttcgttcga ttggtctgtc aattaatgaa aaagtaaaaa agaatatgtt
    10921 ctaggaagag ggcggcacat ctatattttt aagtaacgga cagttcaaat atcaagcatt
    10981 gaaagaggct atcctactgt ttgaaggaat acgaatatat aaaaacgaca aagaggaaaa
    11041 agtagttcat taaatccctt caagtgatga aagaatgaga tctaataaaa cctgatcttt
    11101 catctgtttt tttaataacg taataatttt tttatttttt gtgctttcgc cgttattgta
    11161 gagtgattta gcattcttat ttttaaaaat aactgtgtta tata
//
LOCUS       DACCWK010000081        11188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCWK010000081 DACCWK010000000
VERSION     DACCWK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.084"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            150..1409
                     /gene="lysA"
                     /locus_tag="IVW65_002028"
     CDS             150..1409
                     /gene="lysA"
                     /locus_tag="IVW65_002028"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7900899.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1396..2196
                     /gene="dapF"
                     /locus_tag="IVW65_002029"
     CDS             1396..2196
                     /gene="dapF"
                     /locus_tag="IVW65_002029"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7900900.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2199..3080
                     /locus_tag="IVW65_002030"
     CDS             2199..3080
                     /locus_tag="IVW65_002030"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7900901.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3077..3793
                     /locus_tag="IVW65_002031"
     CDS             3077..3793
                     /locus_tag="IVW65_002031"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7900902.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3799..4494
                     /gene="dapD"
                     /locus_tag="IVW65_002032"
     CDS             3799..4494
                     /gene="dapD"
                     /locus_tag="IVW65_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7900903.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4510..5511
                     /locus_tag="IVW65_002033"
     CDS             4510..5511
                     /locus_tag="IVW65_002033"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7900904.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5508..6869
                     /locus_tag="IVW65_002034"
     CDS             5508..6869
                     /locus_tag="IVW65_002034"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7900905.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6829..7980
                     /locus_tag="IVW65_002035"
     CDS             6829..7980
                     /locus_tag="IVW65_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7900906.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7955..9124
                     /locus_tag="IVW65_002036"
     CDS             7955..9124
                     /locus_tag="IVW65_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7900907.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9299..10678
                     /locus_tag="IVW65_002037"
     CDS             9299..10678
                     /locus_tag="IVW65_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7900908.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10959..11188
                     /locus_tag="IVW65_002038"
                     /pseudo
     CDS             10959..11188
                     /locus_tag="IVW65_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3728 a         1952 c         2497 g         3011 t
ORIGIN      
        1 accattttta ttcttatttt taaaaaaata tttcgaaata tactagacaa ttcagaaaat
       61 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
      121 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      181 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      241 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      301 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      361 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      421 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      481 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      541 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      601 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      661 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      721 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      781 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      841 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      901 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      961 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
     1021 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1081 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1141 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1201 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1261 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1321 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1381 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1441 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1501 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1561 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1621 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1681 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1741 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1801 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1861 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1921 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1981 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     2041 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2101 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2161 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2221 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2281 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2341 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2401 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2461 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2521 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2581 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2641 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2701 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2761 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2821 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2881 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2941 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     3001 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3061 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3121 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3181 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3241 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3301 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3361 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3421 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3481 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3541 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3601 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3661 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3721 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3781 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3841 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3901 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3961 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     4021 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4081 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4141 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4201 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4261 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4321 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4381 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4441 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4501 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4561 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4621 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4681 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4741 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4801 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4861 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4921 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4981 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     5041 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5101 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5161 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5221 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5281 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5341 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5401 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5461 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5521 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5581 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5641 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5701 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5761 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5821 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5881 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5941 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     6001 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6061 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6121 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6181 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6241 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6301 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6361 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6421 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6481 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6541 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6601 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6661 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6721 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6781 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6841 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6901 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6961 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     7021 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7081 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7141 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7201 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7261 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7321 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7381 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7441 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7501 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7561 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7621 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7681 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7741 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7801 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7861 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7921 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7981 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     8041 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8101 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8161 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8221 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8281 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8341 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8401 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8461 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8521 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8581 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8641 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8701 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8761 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8821 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8881 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8941 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     9001 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9061 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9121 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9181 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9241 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9301 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9361 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9421 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9481 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9541 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9601 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9661 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9721 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9781 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9841 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9901 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9961 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
    10021 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10081 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10141 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10201 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10261 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10321 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10381 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10441 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10501 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10561 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10621 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10681 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10741 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10801 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10861 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10921 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10981 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    11041 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11101 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11161 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DACCWK010000082        11154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCWK010000082 DACCWK010000000
VERSION     DACCWK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.085"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            61..1512
                     /locus_tag="IVW65_002039"
     CDS             61..1512
                     /locus_tag="IVW65_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7900909.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1861..2493
                     /locus_tag="IVW65_002040"
     CDS             1861..2493
                     /locus_tag="IVW65_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7900910.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3081..4520
                     /locus_tag="IVW65_002041"
     CDS             3081..4520
                     /locus_tag="IVW65_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7900911.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4815..5033
                     /locus_tag="IVW65_002042"
                     /pseudo
     CDS             4815..5033
                     /locus_tag="IVW65_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5282..5497
                     /locus_tag="IVW65_002043"
     CDS             5282..5497
                     /locus_tag="IVW65_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900912.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5714..5970)
                     /locus_tag="IVW65_002044"
                     /pseudo
     CDS             complement(5714..5970)
                     /locus_tag="IVW65_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6157..7068)
                     /locus_tag="IVW65_002045"
     CDS             complement(6157..7068)
                     /locus_tag="IVW65_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7900913.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7087..8091)
                     /locus_tag="IVW65_002046"
     CDS             complement(7087..8091)
                     /locus_tag="IVW65_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7900914.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8088..10211)
                     /locus_tag="IVW65_002047"
     CDS             complement(8088..10211)
                     /locus_tag="IVW65_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7900915.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10216..11136)
                     /locus_tag="IVW65_002048"
     CDS             complement(10216..11136)
                     /locus_tag="IVW65_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7900916.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3447 a         2104 c         2080 g         3523 t
ORIGIN      
        1 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
       61 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
      121 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      181 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      241 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      301 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      361 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      421 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      481 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      541 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      601 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      661 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      721 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      781 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      841 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      901 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      961 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
     1021 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1081 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1141 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1201 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1261 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1321 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1381 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1441 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1501 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1561 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1621 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1681 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1741 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1801 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1861 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1921 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1981 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     2041 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2101 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2161 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2221 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2281 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2341 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2401 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2461 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2521 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2581 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2641 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2701 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2761 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2821 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2881 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2941 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     3001 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3061 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3121 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3181 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3241 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3301 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3361 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3421 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3481 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3541 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3601 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3661 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3721 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3781 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3841 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3901 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3961 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     4021 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4081 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4141 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4201 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4261 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4321 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4381 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4441 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4501 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4561 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4621 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4681 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4741 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4801 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4861 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4921 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4981 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     5041 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5101 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5161 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5221 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5281 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5341 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5401 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5461 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5521 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5581 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5641 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5701 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5761 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5821 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5881 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5941 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     6001 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6061 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6121 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6181 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6241 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6301 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6361 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6421 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6481 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6541 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6601 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6661 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6721 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6781 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6841 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6901 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6961 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     7021 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7081 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7141 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7201 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7261 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7321 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7381 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7441 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7501 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7561 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7621 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7681 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7741 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7801 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7861 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7921 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7981 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     8041 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8101 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8161 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8221 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8281 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8341 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8401 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8461 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8521 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8581 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8641 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8701 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8761 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8821 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8881 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8941 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     9001 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9061 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9121 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9181 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9241 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9301 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9361 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9421 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9481 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9541 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9601 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9661 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9721 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9781 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9841 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9901 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9961 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
    10021 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10081 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10141 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10201 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10261 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10321 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10381 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10441 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10501 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10561 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10621 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10681 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10741 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10801 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10861 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10921 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10981 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    11041 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11101 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc cacc
//
LOCUS       DACCWK010000083        10984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCWK010000083 DACCWK010000000
VERSION     DACCWK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.086"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            949..1422
                     /locus_tag="IVW65_002049"
     CDS             949..1422
                     /locus_tag="IVW65_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7900917.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IVW65_002050"
     CDS             1431..1658
                     /locus_tag="IVW65_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7900918.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IVW65_002051"
     CDS             2294..2665
                     /locus_tag="IVW65_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900919.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IVW65_002052"
     CDS             complement(2921..3163)
                     /locus_tag="IVW65_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7900920.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IVW65_002053"
     CDS             complement(3195..4097)
                     /locus_tag="IVW65_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7900921.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IVW65_002054"
     CDS             complement(4110..4298)
                     /locus_tag="IVW65_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7900922.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IVW65_002055"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IVW65_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7900923.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IVW65_002056"
     CDS             complement(4913..5806)
                     /locus_tag="IVW65_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7900924.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IVW65_002057"
     CDS             complement(5884..6822)
                     /locus_tag="IVW65_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7900925.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IVW65_002058"
     CDS             complement(6856..7206)
                     /locus_tag="IVW65_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7900926.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IVW65_002059"
     CDS             complement(7239..8141)
                     /locus_tag="IVW65_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7900927.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IVW65_002060"
     CDS             complement(8134..8991)
                     /locus_tag="IVW65_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7900928.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IVW65_002061"
     CDS             9325..10134
                     /locus_tag="IVW65_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7900929.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IVW65_002062"
     CDS             10174..10671
                     /locus_tag="IVW65_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7900930.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3217 a         2051 c         1842 g         3874 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaa
//
LOCUS       DACCWK010000084        10760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCWK010000084 DACCWK010000000
VERSION     DACCWK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.087"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..1042)
                     /locus_tag="IVW65_002063"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="IVW65_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="IVW65_002064"
     CDS             complement(1437..2279)
                     /locus_tag="IVW65_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7900931.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="IVW65_002065"
     CDS             2604..3020
                     /locus_tag="IVW65_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7900932.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="IVW65_002066"
     CDS             3017..3487
                     /locus_tag="IVW65_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7900933.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="IVW65_002067"
     CDS             3501..4274
                     /locus_tag="IVW65_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7900934.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="IVW65_002068"
     CDS             4288..5100
                     /locus_tag="IVW65_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7900935.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="IVW65_002069"
     CDS             5154..6119
                     /locus_tag="IVW65_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7900936.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="IVW65_002070"
     CDS             6256..8979
                     /locus_tag="IVW65_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7900937.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="IVW65_002071"
     CDS             8992..9624
                     /locus_tag="IVW65_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7900938.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="IVW65_002072"
     CDS             9829..10056
                     /locus_tag="IVW65_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP7900939.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="IVW65_002073"
     CDS             10125..10391
                     /locus_tag="IVW65_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7900940.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="IVW65_002074"
     CDS             10381..10650
                     /locus_tag="IVW65_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7900941.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt
//
LOCUS       DACCWK010000085        10615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCWK010000085 DACCWK010000000
VERSION     DACCWK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.088"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(29..727)
                     /locus_tag="IVW65_002075"
     CDS             complement(29..727)
                     /locus_tag="IVW65_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7900942.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="IVW65_002076"
     CDS             complement(720..2072)
                     /locus_tag="IVW65_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7900943.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="IVW65_002077"
     CDS             complement(2295..3695)
                     /locus_tag="IVW65_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7900944.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="IVW65_002078"
     CDS             3856..4593
                     /locus_tag="IVW65_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7900945.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="IVW65_002079"
     CDS             4718..5197
                     /locus_tag="IVW65_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7900946.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="IVW65_002080"
     CDS             5194..5961
                     /locus_tag="IVW65_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900947.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="IVW65_002081"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="IVW65_002081"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7900948.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="IVW65_002082"
     CDS             complement(6864..7466)
                     /locus_tag="IVW65_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7900949.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="IVW65_002083"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="IVW65_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="IVW65_002084"
     CDS             8147..8731
                     /locus_tag="IVW65_002084"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7900950.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="IVW65_002085"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="IVW65_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7900951.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..>10615
                     /gene="prmC"
                     /locus_tag="IVW65_002086"
     CDS             9800..>10615
                     /gene="prmC"
                     /locus_tag="IVW65_002086"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7900952.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNE"
BASE COUNT         3642 a         1909 c         2091 g         2973 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaa
//
LOCUS       DACCWK010000086        10521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCWK010000086 DACCWK010000000
VERSION     DACCWK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.089"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            35..658
                     /locus_tag="IVW65_002087"
     CDS             35..658
                     /locus_tag="IVW65_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900953.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1108..2157
                     /locus_tag="IVW65_002088"
     CDS             1108..2157
                     /locus_tag="IVW65_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7900954.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2154..3035
                     /locus_tag="IVW65_002089"
     CDS             2154..3035
                     /locus_tag="IVW65_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7900955.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3038..3808
                     /locus_tag="IVW65_002090"
     CDS             3038..3808
                     /locus_tag="IVW65_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7900956.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4087..4941
                     /gene="aroE"
                     /locus_tag="IVW65_002091"
     CDS             4087..4941
                     /gene="aroE"
                     /locus_tag="IVW65_002091"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7900957.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5119..6282
                     /locus_tag="IVW65_002092"
     CDS             5119..6282
                     /locus_tag="IVW65_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7900958.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6399..6809
                     /locus_tag="IVW65_002093"
     CDS             6399..6809
                     /locus_tag="IVW65_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7900959.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6983..9211
                     /gene="topB"
                     /locus_tag="IVW65_002094"
     CDS             6983..9211
                     /gene="topB"
                     /locus_tag="IVW65_002094"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7900960.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9349..9666
                     /locus_tag="IVW65_002095"
     CDS             9349..9666
                     /locus_tag="IVW65_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900961.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9826..10062
                     /locus_tag="IVW65_002096"
     CDS             9826..10062
                     /locus_tag="IVW65_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7900962.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10059..10457
                     /locus_tag="IVW65_002097"
     CDS             10059..10457
                     /locus_tag="IVW65_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7900963.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3668 a         1766 c         2229 g         2858 t
ORIGIN      
        1 ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg aagtagttaa
       61 atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa atccaagtaa
      121 gtattttcga cgtcttgttt ttctgctcat cttatcattg ctgttaacag ttgcaatgtt
      181 tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt cgtccatacc
      241 aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca aacaaagccg
      301 ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca tgcttgatgt
      361 ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt atccgatcgt
      421 ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag tgacatttat
      481 tttctttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag gaataactga
      541 ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata tcaaacaatt
      601 agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg ctaattgaga
      661 cattggcgcg atgaaacggt gggtctatat agcgctattt ttaattatca gaacaagtga
      721 tgtttgatct tcaaaataac aaataaaaaa ttaaaaaata tagttgacga aattcaaaaa
      781 ttcgattaga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac ttatcaaaac
      841 gtatttcagc agaaaacaaa aaaaattggc gcagctggcg tagataagtt gtattaatgg
      901 tgagactttc ccatagatac aactttttag agtgcatttc taaagcttgt atctatgcta
      961 gacatgaaca ttgactgccg catagattag aatctatgcg gttttttgtg taaagacaat
     1021 gaccgctttg gccattgtct ttttttgtag actggatact tgcctgatta taataaataa
     1081 aaagattgac tttgcaggag gaaaaaaatg aaaaacaaac gattaataac agttgtagta
     1141 attattttag tttatttagc aggtgcattt atttatggaa aagcctcagg cgaaaaagaa
     1201 agtacttcga cgaaccagac aaaaaaaaca acagttgttg gggtattgca atacgtgagc
     1261 catccggcat tagatgagat ttatcgagga atcaaagatg gtctgaaaga agaaggatta
     1321 aaagaaggag aaaatcttga gatcaaattt caaaatggac aagccgatca gagcaagcta
     1381 gccacaatga gtcaacagct agtacaatct gatccagatg ccttgattgg aatcgcaaca
     1441 cctgccgctc aatcattagc aaacacgact tcatcgatcc cagttgtgtt aggtgctgta
     1501 acggatcctg tgggggctgg attagtcgct tctttagaga aacctggtgg taatgtaaca
     1561 ggtgtttctg atcagccgcc tgttacttca caaatcaagc ttgcttctga tttgttgcca
     1621 aatgcaaaaa aagtcggaat cttgtatgct tcttcagagg ataattcaaa ataccaagta
     1681 gcacaagcag aagaagctgc aaaaaaagaa gggttagaat cagtaaaata tgcggtgcct
     1741 tccactaatg agattgcaca aacggttcaa gtgatgagcc gacaagtcga ttttatctat
     1801 gttccattag ataataccat tgccaatgcg atgcaaacag tggtaaaaga agcaaacaaa
     1861 gcaaatatcc ccgtcattcc atctgtggat acaatggtgg aacaaggtgg actagcaaca
     1921 atcgggatca atcaatatca attagggctt caaagcggta agatggcggc agaacttgcg
     1981 agtggaaaag aaaaaccgga aacgacgcct gtttatacat ttgatcaagg ggatacagtg
     2041 atcaatcaat ctcaagctga tcgcttagga atcattgttc cccaatcgat gaaggaaaaa
     2101 gccaaaatca ttacagatga ctcgcagcaa gaaacatcta aggaggatga caaatgatcg
     2161 tatcagctat ttcacaaggc atgctttggg caatattagg attagggatt ttcatgactt
     2221 atcggatctt agactttcct gatatgacga cagaagggtc cttcccatta ggtgctgctg
     2281 tctgtgtgac agctattact catggggtag cgccgattgt agctacatta ttaggcgtag
     2341 gtgctggcat gatagcagga ctcgttacgg ggttacttta tacaaaagga aagataccgg
     2401 ttattttagc agggatttta gtgatgtcag gattgaactc agttattttg tttgtgatgc
     2461 gaacaccaaa tctttcttta ttgaatcatc cgattctaca agacagcttc catcgctttg
     2521 ggttacctga ttactttgac acagtccttc ttggcttcat ctctttgggc atcttgattg
     2581 ctgtactgct attcttcttc aatacagatt tgggtcaagg ctatattgcg acaggagaca
     2641 acgaaacgat ggctcggtcg ttagggataa aaaccgatcg tatgaaaatt ttaggtttaa
     2701 ctttgtcgaa cggtgtcatt gcattatccg gtgcgctgat tgcccagaac gacggatatg
     2761 cagatgtaaa taaagggatt ggcgtaatcg tcatcggtct tgcttccatc atcatcggtg
     2821 aagtgatctt ccaagaattg acgctagcag aaagattgat tgctattgtt gtcggcagta
     2881 tcatttatca gctattgatt ttagtagtaa tcaaactagg gtttgacacg acttatctga
     2941 agttgttctc cgctatcatc ttagctattt gtctgatgat cccgcaattg aagaaagcac
     3001 tgaacttaaa aacgggtttt gagaaggagg catagtaatg actgatatat tgacaatcaa
     3061 aaatggcaga aaagtcgtaa acaatggaat gaatgaagag aaagtactat tggatgaaat
     3121 taaccttaca atcagagaag gggacttcat cactgtcctt ggcgggaatg gtgctggaaa
     3181 aagcacactg ttcaatacga tcgctggtac gcttcaactc agtcaaggat cgatctcttt
     3241 taaagatcag ctgatcacaa aagattcaga agaaaaaaga gcacgattcc tttctcgagt
     3301 tttccaagac cccaagatgg gaacggctcc tcggatgaca gtagcagaaa atttgctact
     3361 tgcacaaaaa agaggtcaaa aacggaaatt ccgtttacgt aacttgaaaa atcaaaaaga
     3421 agattttttc agactatgtc aagaagtcgg taatggccta gagcaacact tggatactcc
     3481 agcgggtgaa ttatctggtg gtcaacgcca agcattaagc ttgctgatgg cgacgatcac
     3541 agctcctgag ttactattat tagatgaaca tactgcagct ttagatccta aaacagctaa
     3601 agcattaatg cgattaacaa atcagcggat caatgaacaa aaattgactt gtctcatgat
     3661 cacgcatcga atggaagacg ccttgaaata cggaaatcgt ctgatcgtac tccaaaaagg
     3721 gaaaatcgtt caagatattt ctgaaaaaga aaagcaacat ttgacaatgg gcgacctttt
     3781 gcagttcttt gaagaagtag tggattgatt tcgacagaaa aattcctttc agtagaagga
     3841 cttgaaaact cattgacatc ttcctttgat tcatcctata cttaaaacaa taaacaatta
     3901 aacatatttg ggtgttttgt aggatcacgt agtcaatgat tttaacaatt aaatagaata
     3961 ttctttgctc tgacaagtaa acgatgagaa aagaatttgc tactttgcca cctagacgtt
     4021 tcgtactttt tcgtatcaaa cgctaagtgg ctttttttat tgaagtaaga acaaaggagg
     4081 aataagatga tttctggaca aaccaaatta gctgggctct ttgctgatcc ggcttctcac
     4141 agtttatccc cattgatgca caatactgct tttgaagcaa acaacatcga tgcggtgtat
     4201 ttagctttcc aagtaaatcc aatgaatttg aagcaagcag tcgaaagtat tcgaactttt
     4261 gatatgttag gtgtaaatct ttcgatgccg aataaaacag cggttattcc ttatctggat
     4321 gaggtaagcc aggaagcgca attgatcgga gcagtgaata cgatcgtgca cgaaaagggt
     4381 cgtttgattg gctataatac tgatggtgcg ggcttcatgc aatccgtgca agaagctggg
     4441 atttcgatca aaaagaaaaa aatgactcta ctgggagctg gaggtgcagc aaaggcagtc
     4501 actgttcaag cagcgttaga cggcgctgag gaaatcgtag tttataaacg aaacaaccaa
     4561 acattcgctc aagtaaaaga aacattcgaa caactcagct atcgaacttc ttgtaagata
     4621 aaagttcgtg actttgccga tacgcatcaa ttaagaaaag acttgaaaga aagctgtctt
     4681 ctagtcaatg caacagatat cgggatggga aagaaaatca atcagtcacc gctcttagat
     4741 ataagcatga tgcatgagaa tctagcggtt tttgatttga tttatgcacc tagagaaaca
     4801 cgtttattaa aagaagcaaa aaaagtagga gcaaaaggat acaatggact tggcatgttg
     4861 atctaccaag gagcaatcgc gtttgaatta tggacaaagg aaaagatgcc gatccatgtg
     4921 attgaaaagc tgtttgatta aaaaaggata atagataaag atcttttatt ctcgcataaa
     4981 cggtctcaag aaaagtaatc ctttatgtat cgaagcattt atttcgttat tttttattaa
     5041 attatcaaaa aatgataggc ttttgtcgat tggttatcgt atcctttatt tatcagtaag
     5101 aaacgaggag gaaccacaat gagtcggatg gatcgctata aggacatcca taagaaggcg
     5161 aaacctctga agcgggatac ggggagtatt tttgctagaa gagaacgaaa acgaacgcgt
     5221 atggaaaatg aggaagatgc tacccgtatt tatgaaagag agcagcattc atttgaacat
     5281 ttgaaccaat acgagcaaca gatatacaga gatgcaccaa gagcagaaga acaacaaaaa
     5341 aataaaaaat ttttcaatcg gcaaaagaag aacaaaccgt taaaacctcg aagaaagcga
     5401 tcgtggaaaa aaatcattct atcgattttg cttgttttga tcctttattc aggtatctca
     5461 ttttttattg gtaaatatgt agctgaacat gacacaagct tagaggcagc gacttcagaa
     5521 acctttcatg ggcaaaaagc gacgaatggt gcgcataata ttttgatcct tggaagtgat
     5581 acacgcggag aagatgccgg gcgagccgat acgatcatgg tcttacagtt ggatggtcct
     5641 tcccataagc cgaaactgat ttcttttatg cgtgacagct tcgttacgat cccaggggta
     5701 ggacaaaata agatcaattc agcttatgca tacggaggag ccgatctagt gcgtcaaact
     5761 ttagtagaaa attttggaat cgattgtcaa tactatgcga aagtcgattt caaatcgttt
     5821 gaaaaagtga tcgatgcctt attcatgaac ggagtgaaga tcgatgcaga aaaagatctg
     5881 aatctggatg gtgtagatat taaaaaaggg ggccagaaga tggatggaca tgttcttttg
     5941 cagtacgcac gtttccgaat ggatgaacaa ggagactttg gccgtgtcag acgccaacag
     6001 caagtaatga acgctatttt cagccaattg aagaatccat tgaatctgat ccgtgcacct
     6061 tatgcagctg gtaaagcttt aggatataca tccacagatg tcactgcttg gttcttgacg
     6121 aaaaacctct tttcgattgc acgaggagct ggtggcattg atcgaataag tgtgccagta
     6181 gacggttcgt ggaattatgg gcaaagtgat tatgctggca gtattcttga aattgataat
     6241 caaatgaatc aatctgctat tacagatttt ttgaataaat gatcataaaa aagatcaaga
     6301 agaagctgcg caaggctttt gtcatgaatc aatcaaacga ttcactcgta aaaaacgctt
     6361 tcttatggta gatttaacca agagaggagc tgtttacatt ggatatctat ctgaacttca
     6421 aaaatgagtc gcgtgaagcc atcgcttttt atgaaaaagt ttttcagact gaagcatcag
     6481 gtatcaccac atacggtgaa atgcctggaa tagaacaaat gaacttgcca gaagaagcaa
     6541 aaaatctaat cacgaatgca cggctgaaca tttatgatac attagttatg tttgcagata
     6601 cgccagaagg aattggtcct gaattagttg tgggaaataa tgtttcttta gtcatccaga
     6661 caagaaatga agaagaaacg aagcgcctat ttgaaagctt agctgagggt ggtaagattg
     6721 agatgccact ggatgctgtt ccgtgggctc agttgtatgg gatggtaact gatcgatttg
     6781 gcatcaactg gcagattaac caagagtgac agctaaagat gaatattttc tggaaacatc
     6841 aagcagcaaa cggatcatcg tttgtttgct tgatgttttt gtgtctattt cgactatacg
     6901 gtatcttttt tttattgcca gatgcgaaaa tatgttccat aatagagaag tccgcaaaaa
     6961 attaagtaac ggaggtcaga agatgaagac ggtgatacta gctgaaaaac cttcacaagc
     7021 aaaagcctat gccgaaacct tccaacagca aaagcggcat gaagggtatt atgaaatcag
     7081 cgatccattg tttgctggtg aagtaaccat cacttatggt tttggacatt tggtagatat
     7141 ggttgctcca ggagcttatg aagagcgttg ggcaaaatgg tctttagaaa atttgcctat
     7201 tttcccagaa accttccgtt acgaagttcc aatggacaaa cgtgcacaat tttcgatcgt
     7261 aaaacgagaa ttgcagtcag ccgataccat cattattgca acagatggtg atcgagaagg
     7321 tgaagccatc gcgtggtcga ttattcgaca agcaaacgca ttttctaagg aaaagaacta
     7381 tttacgttta tggatcaatt ctcttgaaaa agaagcaatc tatgaaggtt ttaagaatct
     7441 ccaacctgga aagaactatt ttccaaaata taaagaagcg caagcaaggc agaacgctga
     7501 ttggctgatt ggaatgaatg gaagtccatt atatagcttg ctacttcaac gaaaagggat
     7561 tgatggcagt ttttctcttg gtcgtgtaca aacaccgaca ctctatatga tttatcagct
     7621 gcaagaagaa atcaaacact tcaaaaaaga aacgtacttt gaaggagaag gtttaattac
     7681 gacaaacaaa gggcagtttt ctggaaaaat cgtaccaaaa aaagcattta aaacccaaga
     7741 agagttaacg aaggaaatag aaaaacttgg agcgcattta ggtaagcaaa acggacggat
     7801 tcttgaagtt acgaaaaagg aaaagaggtt gcatagtcca cgtttatttt cgctatctag
     7861 tttgcagaca aagatgaatc agctaatgaa agcaagtgcc aaagctacac ttgatgctgc
     7921 tcaaggattg tacgaaagca agtttctaag ctatccaaga acggattcct tttacattac
     7981 agaaaatgag catcagtatc tggttaaaca tctgcaaaat tataaaaaat ttttaggaat
     8041 agaagaagta cagacaactg agatcaaagc aaagaaacga tatgtcgatg cgaaaaaagt
     8101 tcaagaacat catgcaatta ttccgacaaa gacgattccg actcctgcac gctttgcgaa
     8161 gctttccaaa ttacagcaag caatctattt gcaagttttg cgcactactg tggctatgtt
     8221 tgctgcagat tatatctacg aagagacgac cgtaatgacg ggcgtacagc aattaaagct
     8281 aaaaagcatc ggaaagatcc cattacagca aggttggcag gaaatcttac gcatcaagtc
     8341 aaaaaagaaa gaagctcaga ctcttcctcc tatcgtagaa aatgaagaag cagaagtaga
     8401 actgaaaagt atggagaaag aaacacaacc tccaaaaccc tataccgaag gaacgttgat
     8461 cacagcaatg aaaactgcgg gaaaaacgct ggataatgat gaagcgcaag aaattctcaa
     8521 agaagtagaa ggaataggga cagaagctac acgagccaat atcattgaaa atcttaagca
     8581 gaagcattac atcgaagtca ataaaaacga aataactgtt acgccaaaag gaatcactct
     8641 ttgtaaggca gtagccaatg aaccattact gacgagcgca gaaatgactg cgcgctggga
     8701 agggtacttg agaaaaattg gtaaacaaga ggggacacca tctgtttttt tagagaatat
     8761 caaaaaattc atcctacatt tattggcaga agttcctaat caaatcgaca aggtagactt
     8821 ttcagatgag attagcggga cacgtcagat aaaagcgatg gagaagaaaa acgatcaatt
     8881 aggaagatgt ccaaaatgta aaaagggaat agtcatgctt tatcctaaaa tcgctacatg
     8941 cttgaatccg gaatgcgact tcaaattgtg gccaacagta gctaaaaaga agctgactaa
     9001 aacgaatctc agagatttgc tttcaaaagg aaagacaagt aaagttgtca aaggttttac
     9061 tggtaaaaaa gggaaattcg atgcagtttt agtattaaaa gaagatatga caatcggttt
     9121 ttcttttcca tggatcgaaa acagtggaac aaaagcagtt gatggaaaca aagaaacatc
     9181 aagaaaaaat aatgaaaacg aagtagagta aatgaaaaca atgaaatagc ggaatattta
     9241 ttccgaaaag tgagaatata aataccaata aaactaaaaa atatcagtga atcaagcaaa
     9301 aacgttgggg taggctgatt ttatatagat agcaaggagt ttttttattt gaaaaaagtg
     9361 cattcatttt taaattatct tatgggagca atcacgattg tggctgtgta ccaagcattt
     9421 ctaaagtatc ctgattacgg gacgaagttg ttgtcattga cgcacaatca aacagcatat
     9481 tcaccaacgt tgattttatc gactttgact gcaggagcta aaagcgctac ttattttttt
     9541 cataaaacga caagcatgct aaagcgaaag aaacagcgaa aacaattgtt ggtggtggta
     9601 gcggctatga tcattattta ttttctacca gttattacac aagtatttca tcaatatttt
     9661 ggttaagaat acgagagttt gtgacaaaag tcatgtcaca aacttttttc tttgtcattt
     9721 tttcgtaaga aaaagcaaaa taaaagtata tacattgatt aaaagtatat acttggatat
     9781 aatgtactta ctatagaatg agaatggtat agagggtggc gaaatatgaa aaaaatgaaa
     9841 aagtataaaa ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc agaagtaaaa
     9901 gaattcatgg gtgtgcagac aggtgacgca atcagttatg tttttcaatc tgatggttct
     9961 gtacgaatga tcaaagcaca agaagaacct gatattgatt cattagttga ttcgatcatg
    10021 aatcaatatg aagacgcgtt aaaagatttg gttgatttat gaggtatctt tcgactaaag
    10081 aaattatcaa aatcaacgcg aaagttattt tacgtcactc gccaggcgaa atgatcggtg
    10141 taaaagatgc aaatgcatta gatatggccg tcaaacagcc ttcacaagca gtattcaatc
    10201 aagaactcta tcctgaaatc tatgaaaaag cggcgatttt agcaattaat ttagctaaaa
    10261 aacatccttt tcataatggc aataaacgaa ctgctcttgt tgccatgttg ttgtttcttc
    10321 aattgaataa ttgtccagtt gctttttctc gtcaagaagc tgttgatttt atcataatga
    10381 ttaccactag ttcattggat ttcgattcac taaagtccaa gataaccagt tatttacgac
    10441 aaaccgcaat aaaatgaacg aattaaaaac tttatttctc aattagatca gcatgtcatt
    10501 aaggaatatg ctgatttttt t
//
LOCUS       DACCWK010000087        10381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCWK010000087 DACCWK010000000
VERSION     DACCWK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.090"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(34..107)
                     /locus_tag="IVW65_002098"
     tRNA            complement(34..107)
                     /locus_tag="IVW65_002098"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Ile,seq:cat)
     gene            complement(662..976)
                     /locus_tag="IVW65_002099"
     CDS             complement(662..976)
                     /locus_tag="IVW65_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900964.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            1262..1501
                     /locus_tag="IVW65_002100"
     CDS             1262..1501
                     /locus_tag="IVW65_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900965.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1863..2123)
                     /locus_tag="IVW65_002101"
     CDS             complement(1863..2123)
                     /locus_tag="IVW65_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900966.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(2308..2805)
                     /locus_tag="IVW65_002102"
     CDS             complement(2308..2805)
                     /locus_tag="IVW65_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7900967.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2935..3645)
                     /gene="budA"
                     /locus_tag="IVW65_002103"
     CDS             complement(2935..3645)
                     /gene="budA"
                     /locus_tag="IVW65_002103"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7900968.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3658..5322)
                     /gene="alsS"
                     /locus_tag="IVW65_002104"
     CDS             complement(3658..5322)
                     /gene="alsS"
                     /locus_tag="IVW65_002104"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7900969.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5527..6189)
                     /locus_tag="IVW65_002105"
     CDS             complement(5527..6189)
                     /locus_tag="IVW65_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7900970.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(6199..7068)
                     /locus_tag="IVW65_002106"
     CDS             complement(6199..7068)
                     /locus_tag="IVW65_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7900971.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVFTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAAYGFSKLNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            complement(7079..7975)
                     /locus_tag="IVW65_002107"
     CDS             complement(7079..7975)
                     /locus_tag="IVW65_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7900972.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            complement(8028..9287)
                     /locus_tag="IVW65_002108"
                     /pseudo
     CDS             complement(8028..9287)
                     /locus_tag="IVW65_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            complement(9316..9629)
                     /locus_tag="IVW65_002109"
                     /pseudo
     CDS             complement(9316..9629)
                     /locus_tag="IVW65_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            complement(9920..10213)
                     /locus_tag="IVW65_002110"
     CDS             complement(9920..10213)
                     /locus_tag="IVW65_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7900973.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKNILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         3254 a         2108 c         1598 g         3421 t
ORIGIN      
        1 tctaacacag aacgattcaa ttggctacat taatgtaccc tgtaggactc gaacctacga
       61 ccggacggtt atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac
      121 ataaagccat aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct
      181 tggagtgtga ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga
      241 gacagataaa catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga
      301 gagaaaagta gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa
      361 acatttagaa atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac
      421 ttttgttcgt acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt
      481 atggtttaag catgttcctt caaataagtt ttttaattat aaactctaga gaaattaatt
      541 catcctaaca taaaataatt gttcactcaa aagtttttct agaacaatta aaacagcagg
      601 cttcacattc gctatacatt tgtaagaagt aataattcct gtactaaaag gcataattgc
      661 tttaattatt ttttgacttc tcttcaaaca caagaagtag ttgtgtaata attgcagaaa
      721 taaggattcc aataatagat ataaaatttg acatggaaac gtctataaca ttaaaagaat
      781 aaagtgttga agaaagtaaa ctgattgaaa taacccctcc gtaaaaaata gtctgtgaat
      841 atacccactt tttttcagaa gacgtacttc gtttggttct aaaacctatc cctgcatttc
      901 tactcttatc aaaaaaccaa atatttagaa gtaataatat tgctattatt aagataaaat
      961 tactgtaaaa atccaaaagc tatcctccta gcaactattt aaatacccaa aaaaattacc
     1021 tctagataat atcataccta cgttttcact tataaatttt ttcataaaaa aagcgtttag
     1081 aggcataatc gttaatacag atggtaacca atgtatctgg tttgaactgg gatatcaccc
     1141 tctccatata tgtattacta agactaatag atactataaa agacttgata gttttgcatt
     1201 gattttgcct aatactgagc ataggtgtat gctaaacgat actataagga gtgtgtacat
     1261 aatgaaaaga attctgttta tcagtttatc aaatttattg ttacttactg cttgtgggac
     1321 ttctgcatca aagaacatac cttcagatac gcaaatttcg aatagtatcg attcaacaat
     1381 ttctacaaat gagaaaaatt ctccggtaga aacttccgat ataaaagttt ctacctctgc
     1441 aaaaaacagg gtaaagaaac tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta
     1501 gcttcttcat cttcttcagc aaataatgat gatagtttcg cgtttactcc taactaaatg
     1561 caacctttac aaatgcttta cgataacaag cctgagctaa atgatcctga tatcagtttt
     1621 gcattttttg aaatgattgg atcggatgat ctttttaaaa ctactagttt gtctatccga
     1681 aaataggaca gttctggcag tataggtttt tatcgagttt ctccaccagg cctcgttaca
     1741 gagacagatt ctctaggcga accttttgaa taaatcaaaa atcatgaaca atcactgcca
     1801 actaaataaa gtgccttcaa aagcttggtc ttctcgcttt tgaaggcact ttatttaaat
     1861 ctttatgaaa ctaaagtatg ttcatatttg ttgctatagt tatatagata ttctgttgtt
     1921 tccaactcat aaacgtgagt atattttgcc actacttttc cgcatttttc taatatttca
     1981 ttgcgatcga cgtcatgata ttcgtctacg cagatcacaa cacaattttc taatttactg
     2041 attacttctc caaccacttt ttgtttcaag ccaataggtt ggcaaatata tttttttcct
     2101 aaaatgatag tttcgttatt catatttcat ccatccttaa tgttttttca ttatttaaaa
     2161 tgataatacc attaaaatat gattatgaaa atcaataaca cttgtgattt tataaaaaaa
     2221 taaaaaaacc gaaaaacaga aatgcccact gtctttccgg ctctttttat tcttgatttc
     2281 tcttcttttt tgtttcaata gacgatttta gttattaata aatgaaaaag catttatatt
     2341 aaaatttgct aataaaagat ctacatattt ttctagaaat gcttgatcga tttcatcata
     2401 ccctttcgtt actccgcaat cgatatctaa aacaccgatt aaattacctt cttttaccat
     2461 tggcactacg atttctgatt gtgcggctga atcgcaggaa atgtaatttg catgggtttt
     2521 gacatcatca acgatcaatg tttcttgttt ttcagctgat tctccacaaa ctccttttcc
     2581 catcttgata cgtgtgcaag aaactcgccc ttggaacggt cctaaaatca attcattacc
     2641 atcgaataaa taatatcctg caaagacagt ctcaggcaaa gtctcattca acaatgcaga
     2701 tgaatttgct aaatttgcta accagttatc ttctgcttct aataatcctt gttgctgtaa
     2761 taataaaatt tcgtatgcat cttttttctg tttttctgtc cacatgataa aaaccgctcc
     2821 tttttgatct atggagaaga ttatagctca gtttttttgt aaacgctagg tttcctcctc
     2881 aaaaaaacga gatacgtcag gttaatcacc ttgaacgcat ctcgctgtct ttttctattc
     2941 tgcttgttgg atttctgctg ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg
     3001 atcatcaact ggaaaatgct gtacaaggga cgagatgcgg ctaatctcaa cttttccttc
     3061 agccacttca aaatccatta catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa
     3121 gtgaaatccc gctgcagcaa ctccttggaa taattcaggc gtaaagaacc cgactaatgt
     3181 tccttggaca ttgctttgtt ggaattctgg ttgagactct gaaatttttg ctagtcttct
     3241 gtaaggtttt tcttgcttcg gcatgatacg gatgtgcata tttttgaata atccagttat
     3301 cttcactgcc aaaaataggt tttccccggc ttctcttact aatatgtctt ttttcaattg
     3361 ttctccatct gcagtttttg agacagaaaa actagtatct ggcgtaaaat ctgtaattgc
     3421 tgcatagggg gtaagttcac ttccatctaa ttgggcaact gagccatctg atcttccttg
     3481 ataagctacg ccatctaaaa agatgacttc accatcaagc ccatgcaatg ttcccaatcc
     3541 cagagttcct ttttctaata tatgagcaat cgtttctgtt ccatccatca agccagccat
     3601 caaagctcct aatgtcccgt gctggtataa gattttttca gtcatgtcga cacttcctta
     3661 atagaattga tctggtaaga ttgtttctcc caatttctca ttatcacggt aatcaatcgg
     3721 aatatcaata attacgggtc cttctgtcgc aaatccttct tctaaagctt tttctaattc
     3781 ctctacactt gtagctcgga ttcctttagc gccaaaagct tctgcatatt tcacaaaatc
     3841 aactggacca aagtctacac ctgacgcccg ctgatatttc attttttctt ggaactctac
     3901 catattgtag tgtccatcat tccaaatcaa atgaacgata ttcaattttt tacgtaccgc
     3961 cgtttctaaa tcctgggcag agaataaaaa tcccccatcg ccggaaactg atacgatttg
     4021 tgtatttgga cgcaccaatg cagcagaaat cgcccatggc aatgcaacac caagcgtttg
     4081 cataccatta ctgaataaca aatgacgagg ttcataactt ctaaagtggc gtgccatcca
     4141 aatataatga ctacctacat ctacggtaac agtcatatca tctgttactt ttgactgaag
     4201 cgtgttaata acttctaaag gatgcaagat acctgcttct ccttttgatt gaacaatgtc
     4261 ccgctccgtt aattttgctt gaagagatgc taaatattct tttgcatctt ctgacacttg
     4321 ttgaggctct aatgaacctg tcaataaatc aagagtggct gagatatcac cgatcaattc
     4381 tctttcaggc tgcataaaag gatcgatttc agctggcgct tcatcgatca caatgatccg
     4441 cgcatctttt tccgcattcc agtttcttgc ttcatattcg attggatcat agccaattgc
     4501 aataactaga tcacttcgtt tcaaaagcat atctcctggt tggtttctaa ataaacccac
     4561 acgaccaaag aaatgcgctt ctaattcacg tgagatcact ccagctgctt ggaatgtctc
     4621 tacaacaggc aatgctgtct tttcaactaa ttgcctgatg gctaacgttt ctttttcact
     4681 tgatcctcgc atacctacta atagtaccgg tagttttgct tctctaatgc gttcagctag
     4741 gtaacggata tcatctgcag aagcagaacc taatttagga tcgctcaatg gcttgatgac
     4801 attaccttgg acaggtgcat ccactacatc ttgcgggata ctgataaagc tcgcgccttt
     4861 ttttgaactc ttagccatac gataagcatt ggcaatcact tctgaaattg tttctggatc
     4921 ttggatttct gcactgtatt ttgtgattgg ctggaacagt gctgcattat ccatgctttg
     4981 atgggtcaac ttcaatagat cgcttctttt tacttgtccg gcaattgcta aaactgggtc
     5041 accttctgct gtcgctgtta ccaaacctgt agctaagtta ctagcaccag gaccacttgt
     5101 tgcgataact actcctggct ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc
     5161 attttgttcg tgacgagtca cgattaattc aggaccttga tcctctaatt cattaaatac
     5221 accatcgatt tttgcacctg gtataccgaa tacataatca acatgatgat taatcaaact
     5281 ttctactaca gatttcgaac cttgtttcat tttttcagcc attgaacatc gtccttttta
     5341 tgctttattt ttaattatta gttagaaata atcacaaata agtgatacag tttctaaacc
     5401 tgtttcattc aaaagtatag cacgttttat ttcatttgtt tagccatatg tttcaaaaaa
     5461 tgaaaaagaa tgacagtttt tacataaaaa ctgtcattct taattgaaag tttcaattta
     5521 taaagtctac agatccatag ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa
     5581 tacgatagaa ggacctagta gaatacacca ttggggcttt acgaaaaaga agagaaacaa
     5641 acttgtcaat agtaaaaaat ctagtaaact cacataccat tttttcaacg tatagtagaa
     5701 cgctatccgt agaacttgat ttggtttcga cgctggattt ctcacctgaa aataccagca
     5761 gttgatgcta aaggaaactg ataaacaagc aagtaaaaca aacaacggaa tcaaccattt
     5821 tccataagaa aaacgtataa aaaagaaaca atcaaaaatc attatcacac taactatcca
     5881 agcaaatagc caataaaaaa atccctttag ccaaaattgg cgaaacgtct ttatgaacaa
     5941 acgtactggt gaaaaacttt tttcttttgc taattcttct gtcataccta tcatcacgat
     6001 gcaacctgga ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc ttccatctat
     6061 cgacaaaagg aacgcagctg ctatatacaa gatattgaca caccaaaaac aaaggctact
     6121 tgccaatgat tgatacaacc aataaaagaa ttccatataa acatttttgt caaaacgctg
     6181 ctctttcata atcacctcct attccttcat tcctgatgaa gcgatccctt ctacaaaata
     6241 tttttgcatc gctaaaaata taaaagcaac aggaagaaca gaaacaactg aggcagccat
     6301 gattaatgcg tactctgcat caaatagccc aataaacatt ttcaacccta actggattgt
     6361 tttattttcc gtagaagaca aataaatgaa tggccccata taatcgttcc atgtattaac
     6421 gaatgtgatg atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg
     6481 atagatttcc cactctgata agccatcgat ccgcgcactc tcacataaag aatctgggat
     6541 actactgtaa tattgcttca acaagaacac accaaaagca ttgaacgctt gtaatagcac
     6601 caacgaccac aacgtatctg ttaaacccac ttttctcatc ataatgaact gcgggatcat
     6661 ataagactgc caagggacag caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc
     6721 tttaaaattt agtttagaaa acccgtaagc agcaaaacta gaagtaaaca actgaatcaa
     6781 cgtgatcaca acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag gaatacgaga
     6841 ccaaataacc agataattct ctaaatgcca agttttaggg atccattgaa tcggaatcgt
     6901 gaacacttca ttatttgctt tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt
     6961 caataaagct aatacaatca gcagaatcat acccaaccat tgaataaagg gcatgttttt
     7021 tgaacgaatt tttgcttttt ttctttcatt tcgaacactc tcttccatat cacacctcct
     7081 aagtacgacg cttattccaa gaaaattgaa caaccgtaac gcccaaaaca attacgaaca
     7141 aaacaagtga aatagctgaa gcataaccaa aatcaaattt tttgaatgct tcattgtaga
     7201 tttggtaaac cagcacattt gtcgctcgac cagggccgcc atccgtcatc acttgcacaa
     7261 ggtcaaacac cttgaaacaa ttgatgacaa gcatgatcgt tacaaagaag gttgttggtc
     7321 tcaacgaagg taatgtcaca ttcaaaaatt gtttccaacg gtttgctcca tctagcgaag
     7381 acgcttcata tagttctttt tggatacttt gtagtcctgc aaggtataga atcatgtaat
     7441 aacccatccc tcgccaaata ctgacaatta caattgccca aagcgcccaa tttgaacttg
     7501 aagtccaacc tggaggatgt tcaataaatc gtcttaaaaa gatattaata ggtcccactg
     7561 ttggatgaaa caacatattc caaacaacag caattgctac taaggaagta acatacggaa
     7621 agaaaaatat cgtacgaaaa agcttcatcc ctctaatttt ctgatttagt agtaaagcta
     7681 gccctaacga acaaacaagt gtgccaggca ctacagcagc ggtatataaa aaagtatttt
     7741 tcaaagaaat ccaaaatgtc tcatcgttgg ccattttacg aaagttatct agtccagcaa
     7801 attctggcgt tgaaaaagaa tcccatttca taaatgctag tacaagcgaa aacaggattg
     7861 gtattaatgt aaaaacgaaa aaaccaatga aattcggggc gataaacgaa taagcgatcc
     7921 acgttcgttt tctacgcaaa tgatttgtag ctttcttatt tatactattc tccatttcga
     7981 tgacgaaacc aagagtgttt gatctcttgg cttctctcct ttcatgatta ttctttttcc
     8041 gcagctacac gtttctccat atttaccgtt cctttttccg gcgtttcatc tccaaccaaa
     8101 actaagtcat gttcttcttg taaaatctta tcgattgcag gcccatacgt atcaatcgga
     8161 aattcaagtt tgatttcatc aggagaaaaa gcttttttag aaatttcatc cgttggcatt
     8221 ccagcgagat taaaataaag ctgatcaatc tcatccgtac gatatgaagg aactactcct
     8281 actcctgcta atacttttgc tccttcttca ctggctgcaa attcaataaa ttcttgcgcc
     8341 gctttttgtt tctttgagtt tttattgaca gcaaatgctg ttggtgagcc aaacgtcgtg
     8401 accgattctc cttttttctt ctgaggaata gctgtaattc cccattctac ttctgtctta
     8461 cttgcttctt tgtttgcgag tatgccagcc atataccaag ttcccatgta catcattgct
     8521 gctttttgtt cttcaaattg agactgatag gttactttcg ttgatttagc cgtgccaaaa
     8581 tccatctgcg cgccttcttt ttgcatcctc aatgctcgat cataataatc agtcatatat
     8641 taataatctg gttcaacaag attcgcctcg ttttgagcag ctgcaatagc ttggattgtc
     8701 gaacgccaga tatgttgata tgctccgtaa acttgtccgt catttttact aagttttttt
     8761 gctaaatctt catattcgtc ccaagttaga ttttttggat attcgattcc tgcatcatca
     8821 aacattttct tattgtaata tagtacccaa aagtccgttc gatatggcaa tgcatatgtt
     8881 tttccatcga tatcatacat ttcatatgtt tcttcagcag catcagtgtc taattctcta
     8941 attcgttctg tttgatcgac taactgatct cgtaaagcat aatttgaata cgacaaaaga
     9001 tttttcatcg tcaatacatc tgtcgtgtct ccagaagaaa gcatcgtggt gagtttcgta
     9061 tcataatcat ctgaagcaat atctactgct ttcactttga ttcccgtttt cttttcgaat
     9121 gcttcaaaga gcgctttgaa ttcaggtgtt gtttcataat tccatgtcga aacggtaatt
     9181 tcatttgctg attcttcact actgtcttct tgattaccac aagcaccaaa tacgagtgac
     9241 attcctaaaa ctattgatcc aatcatcatt tttttccatc gcttcattat atgaatcctc
     9301 ctcttctctt atacatcacc agtaacagta ccatgtttta gctagacgag tgatcccttc
     9361 tacataaaag taatcgcccc agatcacaca ttcatctaca ccttttaagc tgtttttatc
     9421 ataaacggca tggagaagca gaccgtttga tccgttaact cgtttcgttg tatagttttt
     9481 ctgtaattct tccaagattt tgattgctat ctcttcgtaa tcgaaatgac gaggatctga
     9541 aacaggcagt tgttggaaag ttctagcaat ccacatgccg ctatagctgc tgcagaacta
     9601 tctttttcct ctttgctatc ttctttgaat ctaattttag gaggtacgtt ttggtattta
     9661 ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg attttttcat
     9721 gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc gctcattagc
     9781 ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat agatacagat
     9841 gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa cttttttcaa
     9901 tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc cgaccgtctg
     9961 aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca taatcgccac
    10021 tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc actccatggt
    10081 caaagcgttt caagacttct ttcaatagga tgtttttctt ttttgttgaa cttggtactt
    10141 gttttacttt tccatcattc atgaatgtgt ctaaaacttt cattttttct tgctccgtat
    10201 aagtataaac catcttcttt ctccttttga ttctaagaat actccattta tgttagaata
    10261 ttctggattt tttattaaag cggttactca taagaaagat attatcatag tgctcttttt
    10321 ttactactac ttataggtag atttattcta atccattatt ctcccctacc aataattaaa
    10381 g
//
LOCUS       DACCWK010000088        10295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCWK010000088 DACCWK010000000
VERSION     DACCWK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.091"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            174..323
                     /locus_tag="IVW65_002111"
     CDS             174..323
                     /locus_tag="IVW65_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7900974.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            334..1848
                     /gene="dltA"
                     /locus_tag="IVW65_002112"
     CDS             334..1848
                     /gene="dltA"
                     /locus_tag="IVW65_002112"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7900975.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1845..3050
                     /gene="dltB"
                     /locus_tag="IVW65_002113"
     CDS             1845..3050
                     /gene="dltB"
                     /locus_tag="IVW65_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7900976.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3095..3328
                     /gene="dltC"
                     /locus_tag="IVW65_002114"
     CDS             3095..3328
                     /gene="dltC"
                     /locus_tag="IVW65_002114"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7900977.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3331..4599
                     /gene="dltD"
                     /locus_tag="IVW65_002115"
     CDS             3331..4599
                     /gene="dltD"
                     /locus_tag="IVW65_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7900978.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4740..5822
                     /locus_tag="IVW65_002116"
     CDS             4740..5822
                     /locus_tag="IVW65_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7900979.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5822..6625
                     /locus_tag="IVW65_002117"
     CDS             5822..6625
                     /locus_tag="IVW65_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7900980.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6695..7435
                     /locus_tag="IVW65_002118"
     CDS             6695..7435
                     /locus_tag="IVW65_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7900981.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7446..8450
                     /locus_tag="IVW65_002119"
     CDS             7446..8450
                     /locus_tag="IVW65_002119"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7900982.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8495..10258)
                     /locus_tag="IVW65_002120"
     CDS             complement(8495..10258)
                     /locus_tag="IVW65_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900983.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3310 a         1702 c         2085 g         3198 t
ORIGIN      
        1 tttttaaaag tacgagaaat tataaagaaa aaagcagtga tttgatgtgc tatttttata
       61 aaagctatgt gacaaaactg ttagtaaaaa aacggtttct gtaagctaga cacatgtttc
      121 tttttgtcaa aattagttac actttgtgtg aggttatatg aggagggaat ccaatgaaca
      181 aatggcgttc taatccaaaa ttgaattatt ggggaaaatt catcattcga acaatttttt
      241 atagtgcagt actgctgttc ctgatttatc tgtatcacta taaaaatatt caaggaggaa
      301 cttttatcta taacgaattt taggaggaag agtatggaaa tcaaaacgat tattgaagca
      361 atcgatgatt ggggaataaa tgaacctgat cgagttgctt atcaggcgga tgagacgcat
      421 acttttggtg aattgaaaca agcatcagac gcacttgctt attatttaga aaaaaaggta
      481 gaaggcgatg gaccaattgt cgtttttggc aatttggagt ttgaaatgat tgtctcattt
      541 ctaggggttg tcaaatctgg ccatgcctat ttgccgattg aagagcatac acccaaggag
      601 cgtatcctat cgatctttcg ggtggctaaa ccatctatgg tcatcagtat cggggactgg
      661 atagaagata ttccttcggt tcctgtcata acaaaaaaag aatttcaaga aatcaagaag
      721 ataccagtag gattttatcc cagaaatagc gtaaaaggta cagaaaattt ttatattatt
      781 tttacttctg gtactactgg agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc
      841 agctttgttc aatggacgat ggaagacttt gggatcaagc ctggtatgca tttcttagcc
      901 caagcgccat tttcttttga tttatcggta ttttctattt atcctgcatt agtttctggc
      961 ggaatgctga agccgttaaa taaagcagtt gttcaagact ttcgtcaatt attcgcgaca
     1021 ttgccagcct tgaagttgaa tgtgtgggtt tctactccat catttatgga tatttgcttg
     1081 atggaaccta cattcaatgg agaaaatgtg ccggaattgg acatgttttt attttgcgga
     1141 gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc gattcccaaa tgcacggata
     1201 tataatactt acggtccaac agaagcaact gtagctattt caagtattca gattgaccag
     1261 catgtactag atagttatga ccgactaccg attggctacg taaaaaaaga cacgaaagtt
     1321 tctattgtcc aagaaggtaa acctgtttca aaaggagaaa cgggtgaaat cattattgca
     1381 ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa agacagctgc tgcgttcttt
     1441 tgtcaaaatg acttagcaag ttaccataca ggggatgcag gtagactaga tgaatatggg
     1501 cttttatttt atgaaggacg tatggatcag caggtcaaat tacatggata ccggattgaa
     1561 ctcggggata tcgaacatta tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta
     1621 ccaaaatacc aaggaacaaa agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa
     1681 acgaaacagc cggattttca gttgactaaa tctatcaaag aacaattact agaagttgta
     1741 atggattata tgattcctca gaagttccat tatgtcgatc tgctgcctca gacagttaat
     1801 ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc ctgaatgatg ttacctcata
     1861 tgatccctta tgcagagccg ttttattttt ttcttttagc cattgcgctt gtaccgatcg
     1921 ttctatcttt attgatatgg gaaaaaagat taccagttta tcaatcagca gtgacacttt
     1981 tctttttatt tgtcagcttt ggaggtaatt attggcaaca gggagcggcg ctgattgggt
     2041 atgttttgtg gcagacgata cttgtatggg gctattttgt ttatcggcaa aggaaaaatc
     2101 agacaggatg gttttttgcc gccgttattt tagcgataat tccactgttt ttaacgaaag
     2161 tgactccttt ttttgaacat ggaaaagcat cgttacttgg ttttttagga atctcttatt
     2221 taacctttaa atctgtacaa atgatcatgg aaataagaga tggcatgatc aaaacatata
     2281 agccatttcg ttatatccag tttctgctgt tctttccaac aatctcttct ggaccaatcg
     2341 atcgctatag gagatttgaa aaagatcagc tccatccgcc tgttcgtgat aaatatgttg
     2401 atcttttggg aaaaggcgtc caaaatattt ttgtcggttt tttatataaa ttcattatcg
     2461 gttattattt tggtcaagta ctgcttcctg tagtgggacg cattgcctta cagcatggtg
     2521 gtatttcatg ggcattagta ggttatatgt acgtgtacag tatgtatttg ttttttgatt
     2581 ttgctggcta tagcttattt gcagtcggta caagctacat gatgggatat cagacaccaa
     2641 tcaatttcaa taaacctttt ttatcatgga atatcaaaga attctggaat cgttggcata
     2701 tgacgctgtc cttttggttc cgtgattatg tatatatgcg attgatgttc ttcatgatga
     2761 agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat cggttatttt gctttgtttc
     2821 ttctaatggg tatatggcat ggattgacgt ggtactacat cgtatatgga ctgtatcatg
     2881 cttgtctgat ctgtctgaca gatgcatgga tccgatttaa gaagaagcat aaagagcagt
     2941 tgccttctaa caaatttacg catggcttag ccgtattttt gacatttcaa gcagtatgtt
     3001 ttagcttcct gattttttca ggatttttag ataaactttt ttttagctaa cgaatagtga
     3061 catattaatt tatataacaa aggagaaaag gaaaatggaa gaacaaattt tatcaatctt
     3121 agaagaaata acaggaacag atgaagtaag aaaaaatcgt gatattgatc tatttgatga
     3181 agggttgctt gattctttag catctgttca attattagtc gagctagacg gagagctgga
     3241 tatccaagtc cctgtatctg aattcgagcg agaggattgg agcacaccga ataaaatcat
     3301 tcaacaagca actgcattga agggttgagt atgaaaaaga aactttttgc tattttcggc
     3361 ccggttgttt tagctgttgg cttactgttt gtgtttttta cctcctccta tcaagtagat
     3421 atctctaatc cagaaacgat tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa
     3481 ggagatgcga taaaaaatta tgcattatct gaaaaacaat atattccttt ttttggctca
     3541 agtgaattaa gcagaatcag tccatttcat ccgtctgttc tggcagaaaa ataccagcga
     3601 aactatcgtc catttttgct gggagcaccc gggacacaat cgttaagcca atatatgatg
     3661 atgcgttctg caggagatgc tatgaaaaac aagaaagtcg tctttattat ttctcctcag
     3721 tggttcgtta aaaatggtgt aaaaactgat tactttaata cgtattattc tgaacttcaa
     3781 acatacgact ggttgttttc aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga
     3841 agattattaa ctttctcaaa agtaaaagag aatgatacgt tgacggcgat tctgcaaacg
     3901 atcaaaaaag ggaagctacc tttacctgaa tctttgaatc agctgcgcag ccaatggaac
     3961 atgctgaaga gagaggatga agtattcagc aatattgggt tgaaagatcg acagcaaaaa
     4021 atcgatcatg agtcaaaacg attaccaaaa caataccaag aaacagaatt atccatttta
     4081 gcaaatcaaa ttggagaacg agaaacgacg aataatccat tcgggttgaa aaacgatttt
     4141 tatacgcatc gtatccgagc ccacgagcca gagctcaaac agtcacaaaa aaattgggat
     4201 tatcgttttt cgccagaatt ttctgatttt cagctggtct tggatcaatt agcaaaaaac
     4261 cataacgaag tattgttcat catcccaccg gttaacgaga aatggagcga ttataccgga
     4321 ctatctcaag aaatgttaca aggatttgcc aaaaagatca aatttcaatt aaatagccaa
     4381 ggattcaatc gaatagctga ttttgtcaat caagcgggta ccaactattt tatggaagac
     4441 acgatccatc taggctggaa aggctggttg gctgcagatc aacagatcag accgttttta
     4501 gaagaaaatc atattactgc tagtaagtat catcttgacg atgctttttt tagcaaatct
     4561 tggcaacatc aaataccaga taaactacaa ttaaaataaa gaaaacaggc gagtgttgct
     4621 cgcttgtttt ctttattttt gaatattctg aataaaagat aaaaatacca agtttgcctg
     4681 aacaaaaatc ataagcaatg gtataatcac aatatgaata tagaaatggg ggaaaacatt
     4741 tggatacaaa agaagaacag ctacttattg atttaacttc tgcaaaagga gtacctggaa
     4801 atgaagaaga ggtaagagaa gtattcagag aatatgccaa accattcgct gatgatattt
     4861 tttatgacgg tttaggcagt gtgattgcca aacatggtgc tggcggaggc cccaaggtct
     4921 ttatttctgg acatatggac gaagttggtt ttatggtaac aaaaataacc gaaaaaggat
     4981 tcttggaatt ccaaactctt ggaggttggt gggggcaagt catgcttgct cagcaagttg
     5041 aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg
     5101 ttttaacacc tcaagtaaga aataaacctt atgaaataaa ggatatgttc attgatattg
     5161 gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg tcctggagat atggtcactc
     5221 catatattga atacaaacga atgaatggca gtaaatattt actggcaaaa gcatgggata
     5281 atcgtatcgg gacagctgtt tctctgcgag tattagagaa tctatctaaa gaagcacacc
     5341 caaatgtttt attcgcagga agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa
     5401 caagtacaca ccttgtaaat cccgatattg cttttgcttt agatactggt acagcaggag
     5461 acactccagg aatgacacca aaagaagcag attcgatttt aggaaaaggc ccacagattt
     5521 tgatttttga tgcatctatg atcccgcata aaaaattatt gaattttgtt attgatgtag
     5581 cagaggaaat ggatattcca ttccaatata cagtgatttc tggcggagga actgatgcag
     5641 gacaaatgca tttgacaaga gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt
     5701 atctgcattc ccatacatca gtgattcatg aagatgatta tttgaataca gtgaaactag
     5761 tcacagaatt agtgaaacgg cttgatagtg aaacagttga aaaaattcgg agttatgaat
     5821 aatgaataat agaataccta tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa
     5881 tcaatgggga atcaatactt ggcttgtttt cttatctata atatttacga gcgtcatcca
     5941 gaactttttt gacggtttca gtccttttcc ggaagaaagc atgcagcagc acttattgag
     6001 ttttctagtc gataatttac ttttattcgc attcaactat gcattgtttt acattgcgct
     6061 agtagttgta cggggtggca gaccaaaaag taacctttta ttttcagttt tccaaagaaa
     6121 gtattttggt tcgatacttg gcatcaattt gatcaattca gtcatcaact ggctactcaa
     6181 cagtttaatc tttctgccca tgtttttaat cagcggagtg agtacttata tgcagctgct
     6241 tttcagttca aatgcggtac ctacgggtgt catgccagaa gattttcttg atatcagctt
     6301 tgttttatcg atgataggga tgatttttgt ttcagcactc atcatgagta ttgttggcgg
     6361 tttattccaa tttgctgcat ggacaaagtt cgactatcct gatttgtcgg ttattcaatg
     6421 tttgaaatat ggctggtttt tattgaaaga tagaattggt acgtatattt tgttgcagct
     6481 gtcttttatc ggctggtatt tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt
     6541 cattgtttat gtgaatgtgt cagttgcaga attttatgaa caagcacgga ttgaaaaagg
     6601 aggtccagac gcctatttta cttagcatgt aagcagttta ttcatcaaaa aaataacttt
     6661 tgtggtagga taaatctttg agggggaatg tacgatgaaa ttaactgttt taggatgctt
     6721 aggagcgtat ccttataaaa atcaagggac aacaggttat ttgcttcaat ctgacgggtt
     6781 caatttgttg atcgatgcag gtagtgctac gctgatcaaa ttacaagaac accttgatcc
     6841 acttgatcta gatgctgtga tccttagcca ttatcatcat gatcatattg ctgaccttgg
     6901 tgtattacag tattactggc aacttcatcc taagagaaat ccggagacat tgctttcaat
     6961 ttatggacac acaaaagatt ccattcactt tgaagaattg acgttggaaa atgtctcaaa
     7021 aggacatcct tattttgaag ctgaagaatt aaaaattggt ccttttctga ttacttttat
     7081 ggaaacaatc catccagtag tatgttacgc catgcgtatc gtagaagaaa caacggggaa
     7141 agtattcgta tttacaggtg attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga
     7201 tgctgatttg tttctagcag atacctatct ttttgaagga aatgaacatc atcatgccca
     7261 tttcacttca aaagaagctg gagaacttgc gaaatcagct aacgtaaaga aacttgtact
     7321 tacacaccta cctcagtttg gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc
     7381 aggcaaaggg attgatatag aacttgctgc agttgacaaa gtgtttaaca tttaggagga
     7441 agaacatgat ttttgtacca aatgaaaacc acgacccaag aatcaattta gcaattgaac
     7501 aatatttgct gcaagaaatg cctttagatg aacctatttt gctcttttat atcaatgaac
     7561 cttcaatcat catcggcaga aaccaaaata cgcttgaaga aatcaatcga gattatgtgg
     7621 aagaaaatgg cattcatgtt gtccgtcgct taagtggagg cggagctgtc taccatgatt
     7681 ttggaaacct gaactttagt tttatcatgc ctgatgatgg ggattcattc cgtgactttg
     7741 cgaaagtaac acagccaatc atccaagcac ttcatgaatt gggtgttgct ggggcggaat
     7801 tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg
     7861 ctaccaacgg tcggatgttt gctcatggaa caattatgtt cgactctgat atcaatgaag
     7921 tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc
     7981 gttctcgtgt aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa atgacaacag
     8041 aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa
     8101 aaacgtacga attgactgat gaagattgga aaaaaatcaa tcgaatctct gaggaatatt
     8161 atcgaaattg ggattggaat tacgggaagt ctccagattt caatttctct cgccaaaaac
     8221 gtttttctat cggttcgatt gaagtccatt tgaatgtctc agaaggaatg atcaaagaag
     8281 caaaaatctt tggcgatttc ttcggactag gagatatctc tgacgtggaa aaaaaattgg
     8341 ttggacagaa atatgataaa gcatctctat caaaaatagt cagccaaatt gatgtgaaga
     8401 aatattttgg tgcgatagaa ccagaagaat tgcttacctt actttattga aaataaaaaa
     8461 tagcggaatc cacatatatg gattccgcta ttttttatac aggattcggg taatttcgag
     8521 agagttgaac agtgatccga tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt
     8581 aacgatccgc tacgtctttc atcatcatta agtcaaaacg ttcctgatca tctaattctt
     8641 ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc actatgttcg caacaaatga
     8701 cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt tcgatacagt ggattttcga
     8761 tggcatgagt ttctctaaag gaatgctcta aagcgtggat caaaagttga gccagatcgt
     8821 catcgttgat aggaagttct tcgggaagat ctgcttggaa ggttatctga atattccgtt
     8881 tggcagctaa cttctggtaa cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag
     8941 aacacagcat ttcgaaattt tttgcttctt ctaatatctt ttccacatag tttttagctt
     9001 ttgtttggtc cgtatgcatt aaatcttgca gattctccat ctcttggaca aattccaggc
     9061 gaagtgtatt tacttcttgg atatgctgtc gaagctgctc ataatgttgc tccaacaaat
     9121 catttttgat ttttaagaaa tctacttgtc tccgatcttt gtcggtattt aagatgtgat
     9181 cgattagatt gtagctgata ataacgatta gaatgataat atataaaatc gtagaccaat
     9241 tgatcgttgt ttgatagata cctaatgccg tttggatgta aatcaagcaa tggataatgg
     9301 ctaccaagac gatccaaggt gtacaagcta cataaaaacg gttattctta tatgcttcgc
     9361 caatgcatgc aagtgaagtg actaaagtgc ttataacact agcaatatta gcaatcggca
     9421 ttaattcagg aagttctgca ggagtaaaag ttgcataaag cagaacgccg ataaaaatca
     9481 aaagttggaa cgtacaaaaa attccgtacc attttttata gtaaaacatt cggctccaat
     9541 agtagcagat gatgaaagct gacgttaaga taataaataa atgagataaa aaagaatgaa
     9601 cgaatggttc aaaaagaata ccacttaaaa tatcttctga tacagcttca aatcctaatg
     9661 ccaaagcaaa gcaacttaag agaataagtg agtattcaag acgtttttgt ttatacatca
     9721 agtagatcgt atagaaaaag gtccctatag cgataaaaac agcgattatc atcgtcaata
     9781 cttgcggcac tgattgtgaa taaataaaac taatcaatgg tccagttgtt cctagataaa
     9841 ctttcggtgg cagtccagca tagttctgat aaggagatga gactgtaagg gtcaattttt
     9901 ttccgatata atttgctggt aaatctatca cagccaatga gagtccggga ttgctttctt
     9961 cctgataatc tttccgttgg tataaaatct tttcgcctag atatacagta atccattgat
    10021 ggtttccttt gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa
    10081 tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa
    10141 aatcaggatc atcttttgta tagaaaaccc acgaatcttt tatttctttc atttgattgc
    10201 tgtccgcagg agaaaaaata aaataaaaga aataagcaat tcctgttaaa aataacaatg
    10261 ataatataat gattatgctc cactttatac gtaaa
//
LOCUS       DACCWK010000089         9968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCWK010000089 DACCWK010000000
VERSION     DACCWK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.092"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..429
                     /locus_tag="IVW65_002121"
     CDS             <1..429
                     /locus_tag="IVW65_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7900984.1"
                     /translation="DPEAYPTFKEFLDFLGQLELPGASQEKKNRKEEMESIVESLCET
                     YGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMAMTLIWNQALIQGRRQR
                     RLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            1117..2994
                     /gene="ltrA"
                     /locus_tag="IVW65_002122"
     CDS             1117..2994
                     /gene="ltrA"
                     /locus_tag="IVW65_002122"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7900985.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            3125..3502
                     /locus_tag="IVW65_002123"
                     /pseudo
     CDS             3125..3502
                     /locus_tag="IVW65_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            3502..4707
                     /locus_tag="IVW65_002124"
     CDS             3502..4707
                     /locus_tag="IVW65_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7900986.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            4723..5367
                     /locus_tag="IVW65_002125"
     CDS             4723..5367
                     /locus_tag="IVW65_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900987.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            5378..6184
                     /locus_tag="IVW65_002126"
     CDS             5378..6184
                     /locus_tag="IVW65_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900988.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIEYFQATIDLNEELVMKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            6207..6437
                     /locus_tag="IVW65_002127"
     CDS             6207..6437
                     /locus_tag="IVW65_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900989.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
     gene            6952..7260
                     /locus_tag="IVW65_002128"
     CDS             6952..7260
                     /locus_tag="IVW65_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900990.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            7262..7684
                     /locus_tag="IVW65_002129"
     CDS             7262..7684
                     /locus_tag="IVW65_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900991.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            7704..9293
                     /locus_tag="IVW65_002130"
     CDS             7704..9293
                     /locus_tag="IVW65_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900992.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            9305..9640
                     /locus_tag="IVW65_002131"
                     /pseudo
     CDS             9305..9640
                     /locus_tag="IVW65_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9781..>9968
                     /locus_tag="IVW65_002132"
     CDS             9781..>9968
                     /locus_tag="IVW65_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7900993.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYRSGDI
                     LALNLITKTFVDAAEDYP"
BASE COUNT         3799 a         1519 c         2083 g         2567 t
ORIGIN      
        1 gatccagaag cttatccgac gttcaaagaa tttttagact ttttaggaca actggaatta
       61 ccaggtgctt cccaagagaa aaagaatcga aaagaagaaa tggaatccat cgtagaatcc
      121 ttatgtgaga cgtatggcat gatttttgat ggtcatagta cgattcgaca ccttgaccaa
      181 caacagttgg tcttttttga cattgactct attacaggct tagatcgagg ggtacaacaa
      241 tgtcaaatgt acatggctat gacgttgatt tggaatcagg cattgattca aggaagaaga
      301 caacgacgtt taattaaaga aaaacaaatg gaagaacaag acttcaaacg cttcttggcg
      361 atgattgatg agtgccataa tggtgcgacc tgttctgcag tgaaaagctg tgggttgcaa
      421 gtagtctaaa aactattgta ataactctta taacgagaag tcaaatgagt gggtaacgcc
      481 acaaagggct tccactgata ctccgacatg caacaagtag tttatgcaga ccaacgttgt
      541 gtgaagctcg gtgaagtcgg ctgaaggata ccgtaaggta aaagaaatag tgaataccac
      601 gaaagttctc tagaggtaaa gggtgtatcc gataagtcgg gtgtccaaaa aaacttaata
      661 tggtgagtat gtacaaacgt actgacgaac tagcgaatgt actgtccgaa aggcatagct
      721 cctagaaatg ggagagacag tgagcaaaaa atcactcatt gtgctaactt gtggttgagt
      781 aaaagtcgtt gttatgaaag accatgctgc gttacaggcg taggagaagt tagggggcaa
      841 aagctagcat ctaagttaag gcatgtggat agatataagg gaacagggga agccatcaac
      901 atggagagtg catactcgat gaagtggtag taagaaatat gtgaatgaca aataacttac
      961 tttagtgatg gtgaaagtag agtcacgact gatgaaggtt ctgtaatgga actggaggaa
     1021 caggctcgat tcgtctgtaa aagtcatgaa acccgttcaa ccatgggatt ctcgtatgac
     1081 taaaaaggtc acgagccaat tggagcgtga taaaccatga caaaagaaac aagtccaaaa
     1141 gataggaagt tgcggcatgc agaatattat ggcatgacgg aaatcttcga taagatatac
     1201 tccaaaagtt tgaagggaga taatttcaag aatctaatgc agattatcat ttctgataac
     1261 aacattgtcc tagcatatcg taatatcaag agaaatcacg gtagtgtgac aagaggagaa
     1321 gatggagtaa cgattaaaga tattgaaaat ctcaaccgta atgaattcgt agaaattgtt
     1381 aggaagcgat tcgcacatta taaccctcgg aaagtaaggc gtgtagaaat tcctaaatat
     1441 aatggtaaat taagaccgtt gggtattcca tcaatatggg atagaatcgc gcagcaatgc
     1501 atcttacaag tattagaacc aatatgtgaa gcaaaattca ataatcacag ctatggtttc
     1561 cgcccaaata gatcagcaga acacgcaata gcagattgta tggcccgaat gaatcaaagt
     1621 cacatggaat atgtggtaga tgtagatatc aaaggtttct tcgacgaagt caatcatact
     1681 aaactcatga gacaaatctg gtctatgggt atacgagaca aacagcttct cttaatcatt
     1741 aggaaaatgt taagagcacc cgtagtactt ccaaatggga agttgatata cccaacaaaa
     1801 ggaacaccac aaggcggaat acttagccca ttacttgcga acatcaatct caacgaattt
     1861 gactggtgga tagcaaatca gtgggaggaa aaagatgcca aagagctaaa acatcatttt
     1921 aggaaaaatg gagcttggga taaaggtaat atctatcaca agctcagaaa gagtacaact
     1981 ctaaaagaaa tatacctagt gagatacgca gatgatttta aaattttctg tagaaattat
     2041 aatgaagcga aaaaagtttt ctatgcaact aaaatgtggt tacaggagcg tctaaagtta
     2101 ccaatatctg aagaaaagtc aaaaatcaca aatctttcaa aagaagaaag tgaattttta
     2161 ggctttacga tgaaattagt gaccaaaggt aacaaaaagg ttgttaatac gcatataggt
     2221 acaaaagcac aaagcatgat taaagtgaaa ctaaaaaaac aaatcaaacg aattcaaaaa
     2281 agtcctaata gcaaagatac ctttatctat attggagtct ataacagtat ggttattggg
     2341 attcataatt acttcaaaat tgcgactcat tgtagtgtag caatgcgaaa aattgcaaga
     2401 gaactcagac caagttttga aatacgacta cgagaacaag gaatctcaaa agaaggacaa
     2461 tacggaaagt acgaaggata caagccttac ctacggtcag gacaaatacg atatataaga
     2521 gggtttccaa tattaccagt agggtatatt caacacagac atccgatgtg taaaaaaaga
     2581 aatattaata aatacaccga agaaggtagg gaagccattc ataagaagca acaaaccgta
     2641 gaagaatgga agatatcgtg gttgtgtagc aatccagtgc ttagtaagag agcaacgatt
     2701 gaatttgcgg acaatcgaat ttcaaaattc atcgctcaaa agggaagatg tgcagtaaca
     2761 ggtgaggagc ttatcttatc tgaaatgcat tgtcatcata ttatccctta tcatgaaagc
     2821 aaaagtgata gctatgaaaa cctagtgatt gttactgagg aggttcatcg ggtaatacac
     2881 gctacacaaa gtgaaacgat tgaggaactt ctaaaatatc taaaattaaa tccaaaacaa
     2941 aaagaaaaac tcaatgagtt gagattaaaa gttggtaacg aagaaatatc ttaataaata
     3001 tttgtaggac gggaaaacag aatgtctttt atagacgagg gaacgccgta tgcggtgaaa
     3061 gtcgcacgta cggtgtggac tgggggaaaa gacggagata atatcaaact cttacctatc
     3121 agtatcgatt aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga
     3181 aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc
     3241 agaaaatatg tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac
     3301 gaagatcttt atgcgtatgg atgaatctat tttacgtcat atagaaggat tagttggtaa
     3361 atcaatgaca aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat
     3421 caattttggc tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa
     3481 attgtttgat ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt
     3541 aagcgtcgtg caaagcgacg cttatttttg tggttgtttg gtacttctgc aggtttgatt
     3601 actattttga ttactgtgtt cgtgaccctt tttctaatac ttgcggcggg ttcgattgat
     3661 aattctgatt cggattcaag tagtggtggc gaagctttta caggagaata ttcggaagga
     3721 ttacccattt ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat
     3781 gctgtaggtg cagcagtgaa atataaatta ttaccaagtg tgatcctttc gcaatacggt
     3841 tacgaatcag cgtttggtac atcggcgtcc gcaagaaatg atctcaacta ttttgggatt
     3901 acttggtttg atggttgtct gtttcctaaa ggaactgcac gaggaattgg tggcattgaa
     3961 ggtggctggt acatgaagtt tcccaactca aaagctgcat tttcctatta cggctttatg
     4021 gtagccacac aatccaattt taatgcttgt gtaggaaata aaagccctgg cgcaagctta
     4081 ttgatcttag gacgaggcgg ttatgccgct gcaggaatta cggaagatag tccgtattat
     4141 acaggttgta tgtcaattat cacttctaat aaattaaccg aatatgatga attcgccatc
     4201 aaacattggg gtgaaggtgg caataacaac ggcacgatta ctggtgaatg gacgaatcca
     4261 ttccctggca gcagtttaga taaaagttcc tttagcggtg gtcaattatt tggaacgaat
     4321 ccagggggag agttccgacc aaatggtttc catgacggat tagattttgg ttcagtcgat
     4381 catccaggaa gtgaaattca cgcggtgcat ggaggaaaag tcgtgtacgt tggcaatcct
     4441 ggtatttcag gtttaggtgc atgtgtgatc gtgattaatt acgacggttt gaatatggtg
     4501 tatcaagaat ttgcgaatag tacaggaaat tctcgtgtga aggttggaga tcaagtgaaa
     4561 gttggtcaag taatcgggat acgagataca gcacacttgc atttaggttt cactcgaatg
     4621 gattggcgtc aagcacaagg acacgcattc acagatgatg gtacatggat cgatccatta
     4681 ccatttttga atagcagcaa aaaatagatc aggagaaaaa atatggaaaa taaaacttta
     4741 aaaatcgtct taacagctgc aattagcgtt agtttaatcg gcaatatcgc gttgttttgg
     4801 cagcttaaag aagaacaaga gacaaatacc tttaaaattg aagaagtggc taaaaacgcg
     4861 tctgagaagc tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa
     4921 accatcaatg agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca
     4981 aaagaggaag tcactgaagc acaaagaaag acggcagaag agttcggtca attggccatt
     5041 gataaatcat taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa
     5101 gtgatcgaca aattaagtcc tgcagacgat gatcaccaac atgatgatta cggaagttac
     5161 tcctttacat taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa agaggaacaa
     5221 aactttgtgg tatttgttga ttatacaatc ggcaatccgc aattcaaaga tgtcaaacca
     5281 caaaaaataa aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc
     5341 atagacttta gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagaaag
     5401 acaacgtttc aaaaaacgaa aaagttatct tcaatggctg ggaatcttta tgattctcgg
     5461 ctttttgatt cttgctagtc cgatttatac gaatcagaag gtagatatac cagatgaggt
     5521 aagcacgaac gcctttttaa acacgaaaga gctgacaatg gtttcaaaag aaaaaggagc
     5581 gaaccagcaa ttagtttttg aatttgcgat cgacgatgaa gatcaaagct tattaaaaga
     5641 actggctaat cttaattatc gcgtagaagt aaagacagct aaaggggatt atcaagcaat
     5701 aaaagccaaa gtaatcaaag tttctgatga ttattttgca gtgaagctat ctcatgtacc
     5761 agaagaatat attgctatgc gattaaccat tatacctgaa aaaattgatc caaaagtgga
     5821 tatgcaagaa ccacaagatc tgatttatta cgttcacgaa gataaggtca aagatcaagt
     5881 gaaagatgaa gattatgaac aacatgcgat caattacaaa gtgaaaggat acaaaaaaga
     5941 acaaaaagca gcacaaaaac agattgaata cttccaagca accattgatt tgaatgaaga
     6001 attagtgatg aaactaaaag atcaattgcc ttatcaagtt gcagacgatc aggaaaatac
     6061 tgaaaataag attaatagtt accagcaaga aattgaaaca tcgaaaacgc aaatgaaaga
     6121 tcaagaagag atcattcaaa aacttcaaga aaaaattgat cgtatgacaa aagaaaatat
     6181 ttagaagaat aggtggagta aaagaaatga agcgagcgaa cttggtcggc aaagatactt
     6241 ttgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat
     6301 gtcaagaagt gaatttatct aacacaacaa tatctgagtt gaagaatggt agaaagagtt
     6361 tagatacctt aaaattcaga tcatttgaag ctttgtattt agctgctaaa aaatatttta
     6421 cggctgaaag aaaataaaaa caggaggatt ttatgtgaaa aaagtatatc aaaaatgttt
     6481 atggatcagc tatatcacct taggtatagc tgttaatgag atgggagaaa acattaacca
     6541 cgattctaac acaaacatca agcacataaa acatttaaca ctcctccttt ctggctgaag
     6601 atttcaacca aacaggaaga cagcgtatca attatagcat acactttttc ttaaattcat
     6661 ttttcatatg acaaaatatt tccttctaat cgacaaagtt agaaggattt tttattgtct
     6721 aaatacacaa ataaccttaa tttttaaaga gcataaggaa acgtgggaac actttttaaa
     6781 aaaagtgttc caataggctg tgcctattac gtttctcaaa aataattgtg ggaacaaaat
     6841 atttaaaaat aggatagtga aaggatagca aaggatagta aaaggataga agcaggatag
     6901 cgaaaaggat aggctccttt tcgctatcct ttttaaattg gaggaattaa tatggaagta
     6961 agaataagaa atgtagatga gaaaatcgta agagaaattg atcgaagatg tgacgaacta
     7021 agtaaccgaa cgggtaataa atggagtcgt aacgattatt taaagctact cgttgaaaat
     7081 gattttgatc gtcctttgat ggaatataaa caagataaat ttgatcaatt attaaatcag
     7141 ttaatagata aattttcttt tgttcaaagt cataatacaa aagtattaga agaatatatt
     7201 tgtcagaata atcaaattat cgaactttta attgaacaaa gtagaggagg agaagtttaa
     7261 catggccaaa gttatagaca agcatattta tttttatgaa acacatatag atgttctaga
     7321 tgaaattatt gcaactcatg cagaggttaa aaactatagt gaagccgttc ggtttctatg
     7381 tatgaattat gattcagaag tggagaaaaa agtaaattcc aatcaagcaa agttgaatgc
     7441 aatgggaaaa gaaatttcta ttttaaccga aatggtttca gagtttgcgg atctccattt
     7501 gaaagataca gggattttaa ttggtacaaa ttcaactgtt taccaagaag caaaagagcg
     7561 tgtggaaggc aagataaaag caaatcaaac gaagaaatat tctcctaaga taaagaaaac
     7621 aataagcgaa tcagtgacta caaaaagtga agtatttgat ccaacaaggt tcttaaaacg
     7681 ataaaaatta gaaggagaat gtgatgagta aatcacctgc agtcattctc acttgtcaat
     7741 ttaccttgcc caataaaaaa tctttttcaa catacattga ttatatgaca agagaaaaag
     7801 cgctggaaga aaaggaacga cgtacgcccg aggaagaaca ggaactgcgt ctaatcaaga
     7861 atgctttaga ggatttttat attcctgaag gagaaacttt ttcggagagt aagacgggta
     7921 aagaattcag cgaaacgtcg aatgaagcgc aatcgttgat gaaacatgga atcaattttg
     7981 acaagttaga agaacaagat tttaccaaat atctttctta tatgacacgt cactatgcgt
     8041 tagaacaaaa gaaagtactg acgcatagtg aacaaaaaga ataccaacgc ttgaaccaag
     8101 caataaaaaa atatcaaaca gagccacaca aaaaagcttc aggaaatctt cctggagttt
     8161 tttctatggc ttcagacgaa gtgaaaggaa gtgatctgaa ggagatcaag caaaccttcc
     8221 aaagaggaca acaaaaaggc tcaatcattt atcaagatgt tgtctctcat gatcattcgt
     8281 atcttgaaaa aataggattg tacgatccca aaacagatac tttagatgaa gaaggtttaa
     8341 aagcagctgg aagaaaaatg atggagacat tgttcgagaa agagcaactg aatgaaacag
     8401 gctactggat ggcaacgatt caccgaaata ccaaacatat ccatattcat tttgcagttg
     8461 ttgaaaaaaa aaatacgcgt aaaccataca ctgagattga aaatggtgtg agttatatcg
     8521 taccgaaagg gaagagaagg caagcaacat tagacgaaat gaagactaat tatgtacatg
     8581 agttagaacg tttttcttat gaaaaaaatc gaatgggtat ggagaaacga aatttattga
     8641 cacggaaaag tgatttgcgt aacaccttaa ccaaagtagt gaaagagcca gtacgttatg
     8701 atcgacacgc aatgagctta ttaactgaag tatataaaaa cttacctaaa aagcgttcgg
     8761 aatggcatta cggggatgaa aatcgaacca aactttcacc atacacaaga gagaaactcg
     8821 atgagttaac ccaatatgtg ttaacaaaag aacctggata tcaagaatat atacagattt
     8881 caaaagcatt gaaagaagaa gcaaaaagtc tttatgggga aagcaagaga gaatcaaagg
     8941 atgcagaaaa aaatgctctc tttgatttaa aaaagcgcac aggaaatgcc attctaacag
     9001 aactgaagaa acaggatagt caaataaaac ctttaataga aaggatggat cagatgcaat
     9061 ctatttacaa tgaacgatcc tttcgaacac ttaaacagtt gccaacgaat gaatacaaat
     9121 atagttacga agaattcatg aagcgcagga aacaattcca acaaagagtt gtcacgcaac
     9181 ggagtttgcg tcaattaaat cgaacgatta atgcccatga agaaaaatac cacgcaagta
     9241 aagcatacga agagcaacaa cgaagaattc aaatggaaca agaaagacag taggaggaat
     9301 agcaatgaaa caatatttag atcaatggaa agttatagaa ggatcattaa gagaagaacg
     9361 aatcgaacaa cttcccgatt gtttataaaa agagcatttg tttcagataa gagaaatgtt
     9421 gagaaacgaa cagtttgatc caaatcaatt tttagtcgtt gagtacccag ctactggtgt
     9481 gtactgttgt aatcatgtga agggtgaaaa atattttatc attcaaaaat atgagggcaa
     9541 gttagcgcct tattatacta cttgggaaat gaatgaagag gggatcaata acttcccgtg
     9601 taaaagcatt gaagagtcta tttcactcac agaatgttag ttgttaagtg aataaaagaa
     9661 gtaaaagagt cctgaacata atttgtccag gactcttttt ttcaatgaat tgttttccgc
     9721 caattcattg agtggtgcga tttccgtcta cattatatca aaagaaagga ggaagaagaa
     9781 atggcttatt caacgattgg agttgttgtt gaaaaaagca gagacaatct tgtttttgtg
     9841 acagaaattc aaacaggtag atcttttgtc gtcacagaca aagcggcaaa agcttatcga
     9901 agtggcgata ttttagcgtt aaatctgatt acaaaaacgt tcgtcgatgc ggcagaagat
     9961 tatccatt
//
LOCUS       DACCWK010000090         9948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCWK010000090 DACCWK010000000
VERSION     DACCWK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.093"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            131..3358
                     /gene="fss3"
                     /locus_tag="IVW65_002133"
     CDS             131..3358
                     /gene="fss3"
                     /locus_tag="IVW65_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7900994.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3471..3785
                     /locus_tag="IVW65_002134"
     CDS             3471..3785
                     /locus_tag="IVW65_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7900995.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3798..4172
                     /locus_tag="IVW65_002135"
     CDS             3798..4172
                     /locus_tag="IVW65_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7900996.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4182..4517
                     /locus_tag="IVW65_002136"
     CDS             4182..4517
                     /locus_tag="IVW65_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7900997.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4600..5940
                     /locus_tag="IVW65_002137"
     CDS             4600..5940
                     /locus_tag="IVW65_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7900998.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6018..6692
                     /locus_tag="IVW65_002138"
     CDS             6018..6692
                     /locus_tag="IVW65_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7900999.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6925..7359
                     /gene="dcm"
                     /locus_tag="IVW65_002139"
     CDS             6925..7359
                     /gene="dcm"
                     /locus_tag="IVW65_002139"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7901000.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7360..8067
                     /locus_tag="IVW65_002140"
     CDS             7360..8067
                     /locus_tag="IVW65_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7901001.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            8057..8347
                     /locus_tag="IVW65_002141"
     CDS             8057..8347
                     /locus_tag="IVW65_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901002.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8606..9790
                     /locus_tag="IVW65_002142"
     CDS             8606..9790
                     /locus_tag="IVW65_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7901003.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9805..9924
                     /locus_tag="IVW65_002143"
     CDS             9805..9924
                     /locus_tag="IVW65_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7901004.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3294 a         1679 c         2247 g         2728 t
ORIGIN      
        1 aaatttttag gctcattcat tctcaaatcc attctattca gtcaataaaa attttaaaaa
       61 gactttataa acaaagatgt tcaagcgagc atcttatttt tttgtgctta atttgaaagg
      121 agaacatttc atgtataaaa aacaaaaaaa gaaattatgt gtattaacta ttttgagttt
      181 ggctgttcaa gcaattgttg gagcaattgc tcctacgatt acttttgctg atgaaattac
      241 ccatccacaa acggtaaccg tagaactaga tttagcacat caatatgctg ttgaaggaac
      301 ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg cattatgcag tgtacaacgg
      361 ctttaaacaa gatattttct gtattgaacc aggagtcccg atttacaatg agttcactcc
      421 tggctatgag aaaaacccgt taccagatat gtccgataaa gccaaattag tttccgtttt
      481 atggaaaaaa gtaggtacag atgcagatac tcatattgtg gcacagaaga tgatctggca
      541 agaagttaat gggtataccc ttcactcaat aaaacgatca gatggtagtg ccgtaaatat
      601 cgcagccatt gaggcaaaaa taaatcaagc cattgacgat tatcagaaga aaccaacctt
      661 ccataatagt acggctaaaa cagtattagg gcaatcgact actgtgacgg atacgaataa
      721 tctgaatttg tcagagttcg atgaggttgt ggaaaatgcc gcaaacattg attatcgtgt
      781 gaatggtaat cagttgataa tcaccccaaa tgccaattca aaagaaagtg gtgtgctgac
      841 gcttaagaaa tctgctggta ccggaacacc tgtcgcctat aaaaaggctg gccaacagac
      901 tttaatggcc ggggcgattg ataaacctaa tacctacaca atcaaaattg atgtggaaac
      961 ggagggttct ttaaagatca aaaaagtcga taaagaatcg ggtgatgttg taccaggaac
     1021 ggttttccat ttagactttg gaaaaacttt acctgcaaaa gacgtgacta ctgacaaaga
     1081 aggcattgct acattggatg agattcctca tggtacaaaa gtaaccatta ccgaaaaatc
     1141 agtaccggcg ccttatacga ttgataccac ccccatgact accaccatta aagcaggcga
     1201 aaccatttac gtgacttcaa aaaacgcacg ggaaaaaggg caaatcattc tggataaaag
     1261 cggagttgaa acagggagtg atctttggaa tgacaattat tccttagctg gtaatacgtt
     1321 tgccattcgc aaagacagtc caaccggtga aatcgttcaa gaaatgacca cggatgaaaa
     1381 cggtcatgcg gaaacaccaa aagagattgc taatgcgttg gaattgggaa cctattatgt
     1441 gaccgaaact aaagctagtc atgggttcgt gaatacgttc aaacctgtaa aagtcgaatt
     1501 gaagtatgcc aatcaaaccg tagcccctgt taccagtaac gtaaaagggc aaaatcaaga
     1561 agtcactggc gaaaccactt tgacgaaaga agacaaagat actggggata aagcacaagg
     1621 taaggcagtc tttgagggtg ctgaatacac cttgtttact gctaaggatg gaaaagctgt
     1681 taaatggagt gaggctttta aaccagagtt agcgaaggga acgaaaggtt ctgatgaaac
     1741 ggtcactttg actttagacg aaaagaacca agttgccgtt aaacatttag ctatcaacga
     1801 gtattattgg caagaaacaa aagcgcccga aggatatagc cttgatgaaa caaagtatcc
     1861 tgtatccatc aaaaaggtgg atgatagcga aaaaaatgcc gtgattaccc gaaatgttac
     1921 ggcaaaagaa caagtgattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
     1981 gggcactacc gaaactggct tcaatgatct gacctttaaa gtgtcgccat tggaagggac
     2041 caatgaaatc acaggtgccg aagatgaagc gaccacagct tataacgagc aattagggtt
     2101 tgatggttat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
     2161 agccccagaa ggttttcaaa agattacgcc attagaaatc cgttctactt ttaaggaaaa
     2221 caaagaagac tttgtgaaga gtgagtatgt ctttaccatt accgaacaag accaaaaaca
     2281 accaattaaa acggtgaccg ttccttacga gaagctgacg aacaaagcat tttctgttag
     2341 tttgaaccgt ttaatgctct atgatttgcc tgaggaagaa gatagtttaa cttctcttgc
     2401 gacttggaaa gacggaaaca aagaattaac ttctctggat tctaccgagc tagttgataa
     2461 attgagttac aatctacatg aaatcaaaga agactggtat gtggtagcgc aagccattga
     2521 tgtggatgcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtgattgc
     2581 agaaacaagc gcaacgttgg cgaacaaaga gaaaacagga acttggaaaa ttcagcataa
     2641 attaaccgcc gaacaagttt taaataaaac tattgtctta ttcaactatg tgtatgaaaa
     2701 taaggaagct tttgaagcag gcgataagcc agtagcgaag gatgttagct tgaataacca
     2761 agcccaaact gtcagttgta cagtggaaca ccatgtttct atccaaacaa aggcccacct
     2821 agaaaacggt tctcagacct ttactcatgg tgatgtggtg gatatgtttg atgatgtgtc
     2881 gattactcat gatgtactgg atggctcaaa agaagctttc gaaacaattc tgtatgcttt
     2941 attaccggat ggtacaaaca aagaaatttg gaaatctggc aagattgatt atgaagtaaa
     3001 tgataaagaa ttcaccaaaa ctgtgcttgc gaaaaaagta gataccggaa aatatccaga
     3061 aggaaccaag tttacttttg cggaaatcaa ttacgataaa gatgggaata tcaatggaaa
     3121 acacaatgaa gatttgaaag aaaaatctca aaccttaaca ccaaaagaag ttccaaccat
     3181 actaagtacg ccgaaacaac cggaaacacc aactgttcca agtggctctc aagaatctag
     3241 tcccacagtg aagaccttcc ctcaaaccgg ggagaaaaat tccaatgttt tactgttcat
     3301 tggttttact ttgatttttg cgacggcggg ctattatttc tggaatcgcc ggaactaagg
     3361 tgatgagatg atacaagtaa atcctaagaa ccaacgtgcg cgaccgcctc ctaaaaaagt
     3421 agtcgcgcta tttttgtatc caaaactaac aatgaaaaga ggaaaagaag atggaattga
     3481 aatttgtcgt gccagatatg gctgaaacct ttggaaagtt aagttatgcc ggtgaaggag
     3541 aaattataac agaaggatat ggacggaata ctacggtgat tggtcgcagt tatcatttgt
     3601 attccagtaa gcaacgggcc gatgatattg aggtggtagt agctgcggaa gccggtgaaa
     3661 aggattttga tcaagaccaa ccgctaaaag ccgtgaatcc ccatttggtt gccaaaggct
     3721 atgagattga gaatcgtggg tttactgatt acgtgttgta tgttgatgat ttagtgaaag
     3781 cataggagga aaagaagatg agattaacag aaggtattgt cgtagattca ggtttaacat
     3841 ttgggaagtt gcgtttttcc gcattacgcc gagaagtacg gaagcaaaat gaggatggaa
     3901 caattggtaa tgaggtaaaa gaacgaacct ataatttaaa atcctctgca caagggcgaa
     3961 tgattcaagt tagtatccca gctaatgtac ccttacgcga gtttgcttat gatgcagagg
     4021 tagagttggt caatccaatc gtggataccg ttgccaatta tgtttttcga gaaggaacta
     4081 ccgttaattg gttcattaag gcggatgatt tagtcttgaa gcggcagccg aatcaaggaa
     4141 atcctacgaa ccaaaatgaa agaaagaaat aggtgaagat tatggaaatt aatgttgaac
     4201 atattttaga ttgtctcgat caatatggaa aaggcgagct gactgaggaa caactcgtta
     4261 aagcattaac ctctgatgaa aaaatgtttc tgattataca tcaaggttta cttggagaaa
     4321 atagcgacag tgaagaagac tctgatgtac tagactggtt aggagaagaa gagtctttct
     4381 ttatagttgt cgaagtaaat gaaaacttat gtcggcaggc agaatccatt ttggaagaaa
     4441 ttggtgtgga gatgccggat gctattgaag gctttttaaa tcaattggtt gaaacgaaac
     4501 aactcccctc ctcgtagcag taaatgacta gcataacttt ttgaaattgc ctaatgaaat
     4561 cctattgggc tttttcgatt ggaggtggaa accatcaaaa tgtacaaagg acatcgcata
     4621 cgcgcaggag atcagcactt ggtttatcac tttgttcttg gctggctact cgctctgttc
     4681 atcggttgga tgagcgtctt ttattttcaa gagtttagac aatttgatat ttctaagcta
     4741 tcactttcta ctattgaaat cgtttggtct ataaaagatt tagtatgtct gcttggaagt
     4801 ctcgcttttt caggagctat gatattgctt tatattcact tttttcttga tcattggcga
     4861 agcttgtggc atcgacaaaa gctagctcgg atgattctag agaaccattg gtatgaagtg
     4921 aaacaaactc aaagtgaagg ctttttcaaa gatttgaata gtagccgaac cagagagact
     4981 atcagttact tccccaaaat ctgttatcga atgaaagatg gattactgtc tattcgcgtt
     5041 caaatttcac ttgggaaata ccaagatcag cttttaaaat tggaaaagaa gttagaaagc
     5101 ggactgtatt gtgagttagt agaaaaggaa ctcaaagact cctatgtgga gtatacttta
     5161 ctgtatgata tgatcgccaa tcgaattggg atagacgaag tagtggcaga aaatgggact
     5221 ctgcgattga tgaaaaatca agtatgggct tatgattctt taccccatat gttaatcgct
     5281 ggtggcacgg gtggagggaa gacctatttc ctcctcacca ttatcgaagc actattgaag
     5341 tcagatgcag aattgtttat cctcgatccc aaaaatgcag atttagctga tttaggtacg
     5401 gtgatgcctc atgtttattc acaaaaggaa gaaatttctg cttgtgtgga agatttctat
     5461 gaacgtatga tggctcgtag caaggcaatg aaagaaatgt ctaactacaa aacaggagag
     5521 aattatgcct atcttggact tccacccaac tttttaatct ttgatgaata cgtggcttat
     5581 atggagatgt taacgacgaa agaaagtgcg gtgattttga ataagctcaa acaaatcgtg
     5641 atgttgggtc gtcaatctgg tttctttctg attttggctt gccaaaggcc ggatgcgaaa
     5701 tatttaggag acggaatccg cgatcaattc aactttcgcg tggctttggg tcgtatgagt
     5761 gaactcggat attccatgat gtttggggag gtcgaaaaaa acttctttat gaagcacatc
     5821 aaaggtcgag gttatgtgga tacaggtggc agtgtgatta gtgagtttta tacgccactt
     5881 gtaccaaaag ggtatgattt cttagattca attagtaaat tagaaaagat gaatgattga
     5941 gtgatcttca atactgactt tatacttcaa gtaacgtata atacttttat atagtaggaa
     6001 gaaaggggat aattaatatg gaaagtaatg attcttattt agagtatgca aaagcaaata
     6061 aggataagtt tatttcttat attatcaaag ggaaatcccc cgaagttgga cgagacgcag
     6121 tttttatggc agggagtcct ggggctggaa aatcagaggt tgccattgga ttagctgaga
     6181 attatgataa ccatgtgatt attgatgcgg attactttcg cacccagttt cctgattata
     6241 atggaaaaaa ctctagtctc tttcaaaaag catcttcttg gttggtagag caggcattta
     6301 gatatgtctt agatcatggc tattctttta ttctggatgc aacttttgca atattaagtg
     6361 ctgaaaaaaa tatctcgcgg gcgttgaaaa aacattttcg agtgacgatt ttctatgttt
     6421 atcaagaccc taaaatcgct tggaaattta cacaagaacg tgaaattgca gagggacgtc
     6481 atgtgccgaa aaaggcattt attaacgctt ttttcaaatc aagagagaac attgcaaaag
     6541 taaagagtaa acatccagat gtattgcttc atatcataat aaaagattat acgaataata
     6601 tttccgaagt tcattatgct acggataatc tagaattatt tttaccacta gggtatagta
     6661 ggaaagattt ggaggttcaa ttacatgact gagaaagaac aagtaacgaa aattgtaaag
     6721 aaatacaata agagcattgc ggatttatcg gaaaatgcta ctgcaaaaga gttcaaaacg
     6781 gtgattaaat atgtagccga tcaggccaat gaaaaacaac ggaaattagt tggattaaat
     6841 aaaaaatagt aggacaaatt aataatgtat gagccaagag caaaatgttc ttggcttttt
     6901 tctttgcaat gaatgaggta atgtatgaga tttttagatt tattcgccgg tattggtggc
     6961 tttcgtttag gtatggaaca ggcgagccat cattgtatcg gtttttgtga aattgataaa
     7021 tttgctagac gtagttataa agcaattcat gatacaagta aggaggtgga aatgcatgac
     7081 atcacaagtg tatcagatga gtttattcaa tccctcggac cagtggacat cctttgcggc
     7141 ggatttccgt gccaagcttt ttcaattgcg ggaaagcggc aaagattttc cgatactcga
     7201 ggtactttat tctttgaaat cgctcggttc accactcttc tccgacctaa gcttttattc
     7261 cttgagaacg tccgaggatt gctcaatcac gaaggagggg ctacgttcga gacgatcctc
     7321 cgaacgctgg atggattggg gtatgacgtg gagtggtagg tgcttaactc aaaggcctac
     7381 attccccaaa gccgggagcg gatattcctt atcggacatt ctcgagacgc atgtaccgaa
     7441 caagtatttc ctatcattgg accgtcttca acatctgatc aaaacatcag aaatttggta
     7501 aatatcaatc cttccaatcg aggaatgggt ggacaagtct atggttcaga tggcgtagcg
     7561 ccaaccttaa caggtgatga aggaatcaaa atcgcactgc cagcaaggga tggaatctct
     7621 gtagcaggac tattaccggg aaagtttgaa caaggtaatc gagtttatga agttactgga
     7681 accgccccca ccttgtcaac gaaacaaggt ggtacaaaaa tcatgattcg aaaaaatgaa
     7741 acagaatatc aagaggtgaa gccagaagac agtgtgaatc tggcttttcc taattccaca
     7801 agtcgccgag gtcgtctggg aaaacagagt gttcatacct tactaacagg agaccaacaa
     7861 gcgatcgtca cggatcagta tcaaattcgg aagctaacac ctagagagtg ttggcgactt
     7921 cagggctttc ccgattgggc ttttgatcga gattctcaag tcaattcaga tagtcagttg
     7981 tataagcaag ccggaaactc tgtaacagtt ccggtcattt ttgatattgc cagaagatta
     8041 aaagagttgg atgtacatgg tttatgatga gttgattgga gaaatttatt gggtgattgg
     8101 aaagattcag tcagacccag aattagaaga acaattgcga cggttaaaat ttgatattcg
     8161 caagaatggt gtcaaagtcg ctggcgatcc atatttaatg aatgaggaga cggatgctcg
     8221 aattgaaata aatcaagtaa ttgcagaatt tgagcggatt gcggaccaag caaaagaacc
     8281 agatattcgt caatacttgt tagaaatgaa agcagagttg gaagtaaatg gtattacaga
     8341 tgagtaacct aaaccaacac aactgactgt taggcgctga ccgcggagga ttcgcgaagc
     8401 agaaatcctc cgtggtgctg tgcctgttca tggacggcgt tagccgacca tgatgtctca
     8461 cccccgtatc taacaggggg gtacaaaaac acaaaaaata agtaagcaac cattaagaat
     8521 gcttgttgta tcaaagatag taagtaattt gtataggtgt taactcattt gaaaaagtta
     8581 acaccttgaa tttggaggtg agaacttggc acaacggaat ttagattatc ggctattaaa
     8641 agaccgacgc aacgaatacg gcgtttcaca aaataaattg gctacggctt gtggacttag
     8701 tcggccgtac ttaaatcaga tagaaaatgg tggcgtaacc gcatctataa aaaccatgag
     8761 gaaaattttt gaccaactgg aaagctttaa tcccgatttg cctttgacgt tactgtttga
     8821 ctatgtaagg attcgctttc ccacaacgga tgcacgaaaa attattcaag aaattctcca
     8881 tttgaaattt gagtatatgc tccatgaaga ttatgccttt tactcctatc aagaacagta
     8941 tgtcatgggt gatattgtgg tgatgttgtc tcatgaagaa gataaaggtg tgcttttaga
     9001 attaaagggg cgaggttgcc gacaatttga aactttttta ctcgctcaaa agcgtagctg
     9061 gtacgacttt ttcgaggatt gtctaaaggc cggtggcgtg atgaaacgct tggacttggc
     9121 aatcaatgat cgagtggggc tattggatat acctgagcta acgaaaaaat gtcagaagga
     9181 agaatgtatc tccttgtttc gtacctttaa aagttatcgt tccggggaac ttttaaaggc
     9241 tgatgaaaag gacggcatgg gcaatacctt atatatcggc agtctcaaga gtgaggttta
     9301 tttctgtttg tatgaaaaag actatgaaca atatatcaag ttagggattc cattagacca
     9361 aactgagacg aaaaatcgtt ttgagattcg actgaaaaat gaccgtgctt ttcatgcgat
     9421 tcaagatttg ttaaaaggtc gtagcattga gagcaccacg ttttccatta tcaatcgtta
     9481 cttgcgattt gcggataaag tagaaggtaa aaggcgaacc aactggccct tgaatgaaca
     9541 atgggggcgt tttattggac ggaatcggaa agagattcaa ttaacttccg agccaaaacc
     9601 gtataccatt gaacgaacct tgaattggct aggccgacaa gtcgccccta cgtggaaaat
     9661 ggctaaggag ttggatcgat tgaatcggac aacctatatc caggatatgg tacagaatgc
     9721 acgcttatca gaccgacata aaaagatttt ggagcaacaa agtatggcaa ttgaaaattt
     9781 gattatatga aaagaggaat cgtcatggga attgtaaaag atattttttt ggtatttggt
     9841 ggcagtatgc ttggtgttac tgtgatgtgc ttgatgaatg caagtgcagc agcggatcga
     9901 gcaatggaaa agaaaggaaa gtgacaagat gaatttcgga caaaattt
//
LOCUS       DACCWK010000091         9923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCWK010000091 DACCWK010000000
VERSION     DACCWK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.094"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(83..967)
                     /locus_tag="IVW65_002144"
     CDS             complement(83..967)
                     /locus_tag="IVW65_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7901005.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(1054..1953)
                     /locus_tag="IVW65_002145"
     CDS             complement(1054..1953)
                     /locus_tag="IVW65_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7901006.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(1970..2476)
                     /locus_tag="IVW65_002146"
     CDS             complement(1970..2476)
                     /locus_tag="IVW65_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7901007.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(2665..3285)
                     /locus_tag="IVW65_002147"
     CDS             complement(2665..3285)
                     /locus_tag="IVW65_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7901008.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(3361..3798)
                     /locus_tag="IVW65_002148"
     CDS             complement(3361..3798)
                     /locus_tag="IVW65_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7901009.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            3961..4791
                     /locus_tag="IVW65_002149"
     CDS             3961..4791
                     /locus_tag="IVW65_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7901010.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            4775..6142
                     /locus_tag="IVW65_002150"
     CDS             4775..6142
                     /locus_tag="IVW65_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7901011.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            6161..6970
                     /gene="yidA"
                     /locus_tag="IVW65_002151"
     CDS             6161..6970
                     /gene="yidA"
                     /locus_tag="IVW65_002151"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7901012.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(7013..7456)
                     /locus_tag="IVW65_002152"
     CDS             complement(7013..7456)
                     /locus_tag="IVW65_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7901013.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            7590..7961
                     /locus_tag="IVW65_002153"
     CDS             7590..7961
                     /locus_tag="IVW65_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7901014.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(8130..8912)
                     /locus_tag="IVW65_002154"
     CDS             complement(8130..8912)
                     /locus_tag="IVW65_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7901015.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(8909..9880)
                     /locus_tag="IVW65_002155"
     CDS             complement(8909..9880)
                     /locus_tag="IVW65_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7901016.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         2888 a         2039 c         1746 g         3250 t
ORIGIN      
        1 attgttaagc ctttgcaagt aaggtagtta tgtgttcaac tacttattaa aatgtgttat
       61 ttattaaaga tacttgtctc acttactgaa ctggccgatg atcgtttctg ccataatagt
      121 tctcgacagc cgttggatgc cctaataaat ccccaactgt cgtgatgcga tacccttgct
      181 ctttcagata atcgatcact tgaggaacgg cacgaatcgt ttcaggatag atatcatgga
      241 ataaaataat tgccccatcg tatactgttg cttggatctt ctgcaaaatc aaatcaggat
      301 ttcttgtctt ccaatcttct gaatcaacag accattcgat gatcggtcgc tggatgatcg
      361 ttgctcctac attcgtgaca gaaccatagg gcggacgaac aaaagtgggg gttttgccag
      421 tgactttttt gatctcagtg tctgctttcg tcacttcctc cgtcatcgac tgggcagaaa
      481 gagtggccag attctgatga tcgtatgtgt gattgccaat ctcgtgccct gcttcagctg
      541 tctgcttcac gatttcagga tgctgcgccg tattttctcc taagacgaaa aaagtggcat
      601 gcacgttttt ttcttttaag attcgcagaa gctgcggtgt tgtggtcgct cctggtccat
      661 catcaaagct gattgcgacg cttttgacag ccggatctcg gctcgttttt gtactgtctt
      721 gtgttgattg tgatgactga ctttgtacct tttccgctga ttcttgaatg gcttctttga
      781 cgttagctgt ttcagttgat tggcttttaa tggttgattg ttctgattgt ttgaaagatt
      841 gtgacgaatg ctccgaagag tgtgtttgtc cttttacttc ggtgatcaag gaggccaccg
      901 aagtaatttc gaaaaacaaa agcacggata aagcaataat actgataact aaataatttc
      961 ttttcactat atctcttctt tctctttttc tcttcttatt atttacttgt acttacttga
     1021 aaattgaaac tgctagtcgc aactacttct cgtctaatga tccaaataat cttccgatgc
     1081 caaaatcgtt tttgtcacgg acttaggaat gacaaattct ggtgtaccca tataaccagg
     1141 agcaatttcg tattgagaaa aagtcaaaac taattgatgc tctttgttca aataaaaact
     1201 tcgtgtaggc gtaacaagtg ttggtttttc tatcgtccct tcttgaatat cttgctcagt
     1261 ccaatagtac ttagatggat cttccttgac ttgttcggcc atctgccgtt tgacttcttt
     1321 tgtcaaaaca gatagatagg tgtcatcttt gaataaaagt ggaagtgaga gctggacact
     1381 gtttttcttg tcgattgtgt cgtatctttt ttcaacatgg ctatctgctg ctgtttgggt
     1441 gatctgcctt tcaatcacga gaaaccggtt atcgtctacg attttctgat aatctcccaa
     1501 aacttggtaa tgatccgttt caaaatttcc atattctttt tcagcttttt caaaatctgc
     1561 ttgtccttcg cgaaagtatt tttgatttaa tccatgaatt gttttatttt ctttctcatt
     1621 ttgtgtattg atcttcggta cctgaatatc gattttttca gatcgatcag taaagcgttc
     1681 gcctaagatc aattcaatga ctgatcctaa gatgggtaaa tcttctgcaa agcttcgaac
     1741 ttgactatta aaaagaaggc tacttgttgt aatcattacg atagccaaag atactccgaa
     1801 cccttggaga cgtctctttc tccgctttgc ttttataaat gattgctctt cgataataaa
     1861 acgctggcgg atctcctctt taagcccttt gggaattgct tgttctttat aagttttctt
     1921 tagttttttt aaattttctt tttgggaata catggcactc ttctctcctt cattctagga
     1981 tttgacgtaa agcattcaat gagcggtaca ttttcgtttt aactgtgttt tcattcatat
     2041 ccaagatagt cgcaatttct ttcaaacgaa atccttcaaa aaagtagagg atcagaatct
     2101 ctttttccgg actgtctatt ttatctaaaa tcatatggag ctctgcaacg gaagtttcta
     2161 aaggtaactg ttgggggata tcttgaattt caaataagtc cacaggcgtc cttttttgtc
     2221 tgcgccaact atctaatgcc gtgcggatca atatccgata aaaccatgca ctaaaatgct
     2281 cgatctcttt ttctttttca aaatagttca gggctttatg aaaactatct tgcatcacat
     2341 ctaaggcctc ttctttatca tgtacgtaat tcatcgtgat acggaacatt ttttgataat
     2401 cagtttcgat gagtccattc agcaggtgac ggtatttttc gatctgccgt tttttcttat
     2461 atccagtaaa aatcattctc cactcctttc tgcctcactt aatatgacgt acaaggtgcg
     2521 ctaaaagttt caattttttt attttttatg tatttacttg ggatttttat atacaaaaaa
     2581 gaaggtgtga caaaaatcgt gtcacacctc cttattctga taggctgatc gtctctgtca
     2641 caacctctcg taagaaaata atgtttattc tttttcagag tcagaaaatt cttcctcttc
     2701 ataatcttca tcatcatcgt cttcgtctat gatacttaaa tcttcttcga tatttcctgg
     2761 caagtcttct tcatcatctg tcgggtcatc tgctccgatt tcttgcaagt cagaattata
     2821 agcagcgatt tcttcatctt catcatcatc gtcatcaaac agatcatcgt catcttcttc
     2881 tgtcagatct gtatcttctg ggtcatcgtc attgtaatcg atcgcgtctt catcatcatt
     2941 gataaaggcg ttgactttct tgcgtttacg gcgacgtgga gtatcttcat cctcttcgtc
     3001 cattccatgg tttacttctt catcgatcga atcgatagca taccatgaac gcaagcccca
     3061 acgattatcc cctaatgaga taaagctgcc gtcaatattc aagtcagtat aaaattgtgc
     3121 gagtgaatca cgaatttcac tgtctgattt cccaagatag ttttggatct ggttagctaa
     3181 atcagaaaaa tccatgacat cgccacgttg ttctaagatc gcgtgtgcta cttcgatcat
     3241 tgataattcg cttttgtttg caccttcaaa tacactaatt tccaatcagg acacgtcctt
     3301 tcacagtcta ctccttatca tacaaaatca gacacaaaaa atcaaacttt tcttttatat
     3361 ttacgctgta aaatccaagg tcaaataata ttcgccgatt ttttccggtc ccatgaacag
     3421 atcataatcg attcgaacct tccctgctgt gggacggtct ttcaaactaa catgcagttc
     3481 tttcgtaaaa gtagcaatct ggatcatacc gtatggtgtt cgataggatg tttctaaccg
     3541 ttctttatag ccaaatttca aacgcatccg catttctcct gcacggatca gctgtacatg
     3601 accatctgga actaatttga tcgttacagg aatctcatcg cccgtctcct cctgaatctc
     3661 tttataacgt atgtacagcg tatcgccaat tgtcaccatt tgccctttca aatcaaaata
     3721 aaaatcttgc acttcccctt gctgttttac ttttgtacgc aatttgattg aaatcggtgt
     3781 tccttgtgat aattccatta ttcattctcc tttacttctc atagctacat tgttcctagt
     3841 ttcttttgtt ttgtcaacca acttgtccat ttttacagaa aacctttttt ccaaaacaac
     3901 tctttgattt tagagtataa tattgtagaa aatatgaatt tatacaaagg agccggttga
     3961 atgaaccctg cgattcgact agatcagcac atttatcaaa aaaacgactt ttcccctttt
     4021 gcattaacag atatattaac ggaatattct aaagaacacc agcaaatctt tttacatttc
     4081 tggcaatatg actgcacagt catcttaggt atgaaggatg ttcgaactcc ttttctaagt
     4141 gacgggatcc aaacactgga agatgcaggt tacgctccag tgatcagaaa ttcaggtgga
     4201 ttagggatcg tatcagatga aggaattttg aatatttctt tgattttccc ccaaagtgac
     4261 acaaaaaaaa taaccataga tgacgcttac gagcaaatgc tcctattgac taaatacgct
     4321 tttccaaagg caccgataca agcattcgaa atcacccatt cttattgtcc tggaacttat
     4381 gatttaagca tccatggcaa aaaatttgcc ggtatcgctc agcgtcggat caaaaatggg
     4441 atttctgtta tgatgtactt aagtgtcaac ggtaatcagg aaaaacgtgg gcaagtcatg
     4501 aagcgatttt atcaatctgc tttaaaagaa gactttggta caaatggcta tcctgcagtg
     4561 gatcctgcta caatggcaac acttgaagaa ctgcttgacc aacccctttt tgtaaaagaa
     4621 gtcaaagaag catttgcaaa agcttttgaa aagctgtatt ctgcaagtga tttacaggat
     4681 tctaccgact ggctaaaaaa agaaatccag ctggagcaat ggaacaacca aatccagaga
     4741 atggaacaac gtaataacat aaaggagcta cattatgaca accccttata aatatcaaat
     4801 gctgcctatg gaaaaagttt ttcgtgatcc agtccataac tatatccatg tccagcatca
     4861 agtaatctta gatttgatca attcgaaaga agtccaacgt ctacgacgca ttaaacaatt
     4921 aggcacttct tctttcactt ttcacggagc agaacatagc cgttttactc attcattagg
     4981 cgtttatgag atttcaagac gaatctgcga tatcttttct cgaaattttt caaaagaaaa
     5041 aataggcaac ggaggctggg atgatcagga acggctggtg actttatgtg cggcactgtt
     5101 gcatgatgtc ggtcatggtc cttactctca tacatttgaa catatcttcc atacgaatca
     5161 tgaagccatc actgtagaga tcatcacttc tccagaaaca gaagtgtatc agattcttaa
     5221 ccgagtagaa gaaggttttc ctgaaaaggt agctagtgtg atccaaaaaa cttaccccaa
     5281 cccacaagtt gtccaaatga tttctagtca gatcgatgca gatcgaatgg actatttgct
     5341 tcgggatgct tatttcactg gaacagaata tggaacattt gatcttaccc gaatattacg
     5401 tgtgattcgt ccttatgaag gcgggatcgc tttttccatg agtggcatgc atgcagtgga
     5461 agattatatc gttagccgat atcaaatgta tgtgcaagtg tacttccatc ctgtttcccg
     5521 tggtatggaa gtaattttgg agcatctgct tcatcgcgca cacgagttgt atcaagaaga
     5581 tgccagcagt tttgaactcc attctcaatt acttctgcca tttcttgacg gaagctttac
     5641 tttagaagaa tacctcaaac ttgatgatgg ggtcttgggc acttacttta ctcaatggtc
     5701 tgaatctgct gatcctattt taaatgattt agctaaacgt tttttgaatc gtaaaccact
     5761 gaaatctgct actttttctg gcaaccggga ttcaaaatta gtccaagaac tcactttgtt
     5821 agtagaaaag gtcggatata atcctgttta ttacactgct gtgaattcta gctacgatct
     5881 accttatgat ttttatcgcc cagaacaagg cagacatcgc acacaaatcg agattttgcg
     5941 aaatgacgga actttgatag aattatcaca ggttagtcag cttgttgctg cattagcggg
     6001 acaagaacaa ggagatgagc gatttttctt tccaaaagaa atggttgatc cgtccttgcg
     6061 tgatcattat gatttatttg acgaaactta tcaagaattt gccagccata tccgtaatgg
     6121 cgcattgatt gaaataaatt aaaatatatt ggaggaaatt atgtctatca aactagtcgc
     6181 tatcgacatc gatggtactt tattaaatga gaaaagagaa gtaacaaaag aagtaaaaga
     6241 agcgattgcc gctgctgttg caaaaggtgt ttcgatcgtt ttatgcactg gccgtccctt
     6301 accaggtgtc caggaacagt taaacgagtt gaaccttttt caagataatg attacgttat
     6361 tacttataac ggggcactcg tacaacaaac gaaatcaggt aaaatcatcg cccgtcacgg
     6421 actgacacat gaagatttcc tagagatcga agtaatggca cgccgtgtcg gcagtcatct
     6481 ccattcgatc gatgacaaag cgatctacac gcctaatcga gatatcagcg cttatacgat
     6541 ccacgaagca agtttagtca agatgccgct aaaatatcga acacctgaag aaatgacacc
     6601 agacatgaac atcgtcaaaa tgatgatgat cgacgaacca gaaattttag atgcagcaat
     6661 tgcgcgttta ccgcaaactt tccgtgataa atacacaacg gtcaaaagtg cgccctatta
     6721 cttcgaagta ctaaataaag aagcaagtaa aggtgcggca gttgcgaatc tcgcacagca
     6781 ccttggtatt gatcaagatg aaatcatggc tatcggagat aacgagaacg acttatccat
     6841 gattgaatat gctggtctag gagtagcaat gggaaatgcc gtttctcttg tcaaagaagc
     6901 agcaaatgtc atcacttcat caaacgacga gcacggtgta gctgaagcaa tcaaaaaata
     6961 tgtactgtaa taaataaaaa aactgagaga aagctgatct actcgctttc tttcagtttt
     7021 tatttttttc gatccatctc tcgactgccc atcggtggct accacgtttc aatttcacaa
     7081 gcgcttcttg agggctcacc caccaaattt tattggtctt ttcagttggt tctcctatac
     7141 gcttccaaga atcagcaaca aagaaatatc ctggattata ataataagtt gcacgataat
     7201 tcgagtaaaa atactcatca gcttctccca gataagaaga aatttctgcc acaattccca
     7261 tttcttctat cagctcacgg acaatcgctt cttctttcgt ctcttctcct tcaatttccc
     7321 cgccaggaag aaaaaaagca ccatttggag cctcgattac tgcaatctgc tgatctcttt
     7381 ctatgacaat atatgctgca taacgggttt gataatcagc aaattgcttt ttttcaccaa
     7441 aaattggttt atccatgaat agtttccacc ttaacagttt tattttctat ccattttaca
     7501 tgagaaattt ctcatgaaca agcacaaatc aactatttcc ttacttcttt ttgttatgat
     7561 tgagtaaaag gaaaaggaga gatgaatcta tgaaaatggc tcacacatgt gttcgagtga
     7621 aagatcttga agcatcaatc gaattttatc aaaaggcatt tggttttgaa gaaagccgcc
     7681 gccgtgattt tccagaaaac aaatttacac ttgtttattt gacacttcct ggggatgact
     7741 atgaattaga gttgacttac aactatgatc atggtgcgta tgatctagga gacggctatg
     7801 gtcatatcgc tatagcagct gatgatctag aaaaattaca cgagcaacat aaagctgctg
     7861 gtcttgagat tactgactta aaaggattac ctggcactgc gccttcttac tactttgtca
     7921 ttgaccctga tggttataaa atcgaagtca tccgaggata gttagtatgg ggcattttac
     7981 tcttgattga ataaaaaaca aacaccaata attaaaatga gacgcaaact tttcattaaa
     8041 ttgaaaaagt ttgcgtcttt ttttaacaac cattgtagct ataaggttgc cactctccac
     8101 caatttattt ttaattcatt tgaaagaaat caaataaacg aggtaccttt agaaaacaaa
     8161 actgcttttc ctcctattat cacacgatct tcttttaatt cacattctag atccccgccc
     8221 cttgatgaca tttgataagc tgacaatttt gttttattca ttctttctgc ccaataagga
     8281 ataagattag aatgtgctga tccacatact ggatcttcat tgattgttaa ttttggaaag
     8341 aaggttctag aaacaaagtc tttttgcaca ctttctgctg tcacgatgac tcctattcct
     8401 aattcaaaat ttttgatagc tgtaaaatct ggctgcaatt tagccaccgt tttttcatct
     8461 tctaatacga aaaataaatc acgtgctaag taggcttctt taatcttagc cccaatcgct
     8521 tcttcatatt cagacaatat cggcactttt ttcggcataa tactaggaaa atccatgtaa
     8581 taataatctt taattcttgt cacaaataga ttaccgcttt gagtagaaaa tttaatcgtt
     8641 tcatcaggta ttttgtaata gttaaataaa acaaatgctg ttgctaatgt cgcgtgacca
     8701 caaagatcga tttctcgatc tggcgtaaac catctaagct caaattcttg gtttttcttc
     8761 actgtaaatg ctgtttcaga taaattgttt tcaatggcaa ttttttgcat cgtaccttct
     8821 ggcagccatt cttctagcac ataaaccgct gctggatttc ctttgaacac ctcatctgca
     8881 aatgcatcaa ctacgtaata atctaagttc attttaaagc ccctttctct aatttgttat
     8941 aaatatattc tgcacaacaa agatcttcaa tagctagacc aactgcgtcg aaaatcgtcg
     9001 gcctcgattc tcctttataa aatgtttttt tctcagaaac taattcgcct aatgtaccaa
     9061 caatacaaga ttcctctaaa tctccttctg ccatggctat aagcaggtcc ccgctttctg
     9121 ttaaggcagc tgagtaatca tctacataga tttgagaagt ttccatcaac gatgtactta
     9181 tctctcttgt atcaggagta aatgtgccca cagcattgat atgtgcgtct tcccttaccc
     9241 actctttttg aagaaatgcc tctttactag aggtcaacgt acaaatgatt tccgctggac
     9301 gtactgcatc ttctactgat attgcattta taaacgttat tttaggatac tgttttttag
     9361 ctttagcgat aaaagtattt cgtctttctt ctaaaatatc gtaaacaaac actgtagtga
     9421 tgggacgaat ggtagaaatc gcagtcaaat gcgacaatgc ttgttgacca gctccaatta
     9481 aagcaagtgt ttctgcatgc ggatttgcta agtaatctgt tgccagcgca gatacagcag
     9541 ctgttcgaat ccaggtgact gcatttgcgt ttatcaatgc tttgggtagt ccgttttgag
     9601 catcaaacaa gaaaatctct cctaaatgag aaggtaaatg atactgatga ttatccggaa
     9661 aagcggtaat gacttttgtg ccaaaatatc tgttctcccc caaataagca ggcatcatcg
     9721 caaacatatt tttgcctttt ccgtcaggta aaatttccac caatcgagga ttttggctga
     9781 ttttaccact ttcataatca aaaaaacatt ttttcattac ttgaatcgct tcttcaaaac
     9841 taaaagaagc tactacttcc tcaaaattaa taattttcat agacaattcc ctcctcacaa
     9901 aagattaact tctaaaatca ata
//
LOCUS       DACCWK010000092         9895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCWK010000092 DACCWK010000000
VERSION     DACCWK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.095"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            168..1040
                     /locus_tag="IVW65_002156"
     CDS             168..1040
                     /locus_tag="IVW65_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7901017.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1037..2995
                     /locus_tag="IVW65_002157"
     CDS             1037..2995
                     /locus_tag="IVW65_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7901018.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3327..4178)
                     /locus_tag="IVW65_002158"
     CDS             complement(3327..4178)
                     /locus_tag="IVW65_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7901019.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4301..4768
                     /locus_tag="IVW65_002159"
     CDS             4301..4768
                     /locus_tag="IVW65_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7901020.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5031..5513)
                     /locus_tag="IVW65_002160"
     CDS             complement(5031..5513)
                     /locus_tag="IVW65_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7901021.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5613..6476)
                     /locus_tag="IVW65_002161"
     CDS             complement(5613..6476)
                     /locus_tag="IVW65_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7901022.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7074..7949
                     /locus_tag="IVW65_002162"
     CDS             7074..7949
                     /locus_tag="IVW65_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901023.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8094..8246)
                     /locus_tag="IVW65_002163"
                     /pseudo
     CDS             complement(8094..8246)
                     /locus_tag="IVW65_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(8514..9845)
                     /locus_tag="IVW65_002164"
     CDS             complement(8514..9845)
                     /locus_tag="IVW65_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7901024.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         3112 a         1967 c         1718 g         3098 t
ORIGIN      
        1 attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc atgaaaaatc
       61 tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg gccgcaatca
      121 actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa
      181 agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa gtagctattg
      241 gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac
      301 ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt aatcagattg
      361 atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact gttttgatgg
      421 aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt tctgcggaga
      481 acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa gtgctttaca
      541 gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag cttgggattc
      601 catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga cgaagcggtc
      661 ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc atccatttag
      721 tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat tattcaagac
      781 tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat gctgttttag
      841 acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag atttctaaaa
      901 aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa gaactgggtt
      961 acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc
     1021 atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc ttcaacttcc
     1081 agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt gggcatctta
     1141 tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt taggtcaatt
     1201 acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat caggtgctgc
     1261 tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa ctgattcagt
     1321 agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc agatcagtaa
     1381 cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc gtttccacga
     1441 agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc caatcttggc
     1501 tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc cggtttactc
     1561 tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg caggtcttta
     1621 tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga actccgtgtt
     1681 ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg aaggaacaaa
     1741 aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg ggcttccagc
     1801 aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt
     1861 gatgttagca gcagcttttg cttcattctt tactggtgta acagaaccac ttgaattctc
     1921 atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg ggatttcttt
     1981 agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg gatttgtcga
     2041 ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga tcgtccaagg
     2101 attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa aattccatct
     2161 gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa cgactaattc
     2221 agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg gagccgacaa
     2281 tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag atacaggcct
     2341 gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca tcgacggaaa
     2401 aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga tggcaaaact
     2461 acatggtggt gctgcaacac gacctgcaca aacaaacacc gtggttaaaa cagaaacatt
     2521 tgccggtgga acgatcgaac aagatattta cgctatcgca aatgggaaac tgattccaat
     2581 cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct atgctgtatt
     2641 accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca ttttcccaac
     2701 gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc atatgggcat
     2761 caacactgtt gacttaaaag gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt
     2821 cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg caggcaaaaa
     2881 cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc
     2941 cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag cctgagccat
     3001 cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa atcttgtctc
     3061 agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa taagtccagc
     3121 caaacgtaac atatgaagag ctatttttta tttgactctt cttattactt gatgcagaac
     3181 agctttttta caacctctga taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa
     3241 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttcatact caaaactaat
     3301 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
     3361 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
     3421 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
     3481 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
     3541 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
     3601 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
     3661 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
     3721 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
     3781 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
     3841 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
     3901 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
     3961 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
     4021 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
     4081 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
     4141 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
     4201 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
     4261 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     4321 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     4381 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     4441 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     4501 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     4561 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     4621 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     4681 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     4741 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     4801 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     4861 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     4921 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     4981 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     5041 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     5101 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     5161 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     5221 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     5281 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     5341 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     5401 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     5461 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     5521 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     5581 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     5641 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     5701 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     5761 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     5821 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     5881 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     5941 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     6001 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     6061 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     6121 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     6181 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     6241 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     6301 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     6361 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     6421 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     6481 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     6541 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     6601 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     6661 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     6721 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     6781 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     6841 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     6901 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     6961 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
     7021 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
     7081 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
     7141 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
     7201 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
     7261 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
     7321 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
     7381 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg
     7441 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
     7501 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
     7561 tcatggagat gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct
     7621 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
     7681 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
     7741 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
     7801 tcgatcatac tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact
     7861 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg
     7921 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
     7981 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
     8041 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
     8101 tcatgacaaa tttccttcgt tgctgggtag attttccggc attgttgata gattttatga
     8161 tacttttcta cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga
     8221 caacaacttt caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga
     8281 ttaataatat taacagtctt tatttttcac caccaaaaac ctatctttgc atatactaaa
     8341 tagacgtata tacacagata ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa
     8401 atgtttgttt ttctacatgt tcgtacttat caaaaaatat gatccttatt gaaattactg
     8461 gctatttata ttccaacgac ttcttgtgtt aatttttgtt atctcatagt aaattatgtt
     8521 tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata
     8581 aacacacttg cggctataat taatatttgt aaaacagctg ctcgccagtc tccggctgtt
     8641 gctaaaaatc ccgatataac tggtggcgta gtccaaggaa tttgcactac tacatgatta
     8701 attaatccca cagcagttgc aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca
     8761 aagggaataa tcagactgat attaaataca ataggcaatc caaaaataat aggttcgctt
     8821 atattgaata tagcgggagt taccgataat ttagcaaagt ccttataatc ttttctacga
     8881 ctgaagataa atattgcaat caataaagca atcgtatttc ccgatccacc cattaccgca
     8941 aaggtatctt taaaggccat attgataata tgtggaattt gctctttgtt tgcataagct
     9001 gccatatttt cttgcatatt ctgaatcaaa aacggatcta acaatgaacc tgatattact
     9061 gcttgatgga taccaaatcc aaaaaataga ttagcaatag atgtaattaa caaaaatcct
     9121 ggtaaaccag ttgtaatttt acttaatggc tctgtaacta tcgtggtaat tagcttattt
     9181 atatccatat taaacaattg aatgattata aatgacaata aagcaaacga ggtaatcgta
     9241 ataatgattg ggattaatac attaaaagac tttaaaacag cgggaggaat atttccggat
     9301 agctgaatct gtagtttctt attattagaa agtttaataa ataattctgt agccgctaat
     9361 ccaacaaata tacctacaaa cattcctgaa gctcctgtat aggatatagg tatcacaccc
     9421 gtcacttcaa cagctttatc cgcattttca ggagtaaaca tcatctgcat aggtgttaat
     9481 acaacaaatg tcgaaatagc aactaatacc gctgcaatat gatttgtata atttctgctt
     9541 gcacacatat gataagatac tgctgctaca actaataatg tagttatgct taatgtacca
     9601 ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg tactagtatc gacaatttcc
     9661 cccatgaatc ctgttggttc taagatcact gaattaataa gtgtcacaaa tcctgctaac
     9721 atcataaatg gcataaatgt agcaaacgca tctcgcaaag ttcttaaatg gatctgattc
     9781 cccaacttag tcgcaactat accaaatttt tcaaatacaa tgtccttcct gctacttttg
     9841 cccatatttt ttctccttta tgcttattgt aatcgattac tttttccacc caaat
//
LOCUS       DACCWK010000093         9737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCWK010000093 DACCWK010000000
VERSION     DACCWK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.096"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1251)
                     /locus_tag="IVW65_002165"
     CDS             complement(<1..1251)
                     /locus_tag="IVW65_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7901025.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
     gene            1464..4109
                     /gene="polA"
                     /locus_tag="IVW65_002166"
     CDS             1464..4109
                     /gene="polA"
                     /locus_tag="IVW65_002166"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7901026.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4154..4990
                     /gene="mutM"
                     /locus_tag="IVW65_002167"
     CDS             4154..4990
                     /gene="mutM"
                     /locus_tag="IVW65_002167"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7901027.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4954..5583
                     /locus_tag="IVW65_002168"
     CDS             4954..5583
                     /locus_tag="IVW65_002168"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7901028.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5635..5967
                     /locus_tag="IVW65_002169"
     CDS             5635..5967
                     /locus_tag="IVW65_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7901029.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6279..6770
                     /gene="nrdR"
                     /locus_tag="IVW65_002170"
     CDS             6279..6770
                     /gene="nrdR"
                     /locus_tag="IVW65_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7901030.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6793..8166
                     /locus_tag="IVW65_002171"
     CDS             6793..8166
                     /locus_tag="IVW65_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7901031.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8166..9092
                     /gene="dnaI"
                     /locus_tag="IVW65_002172"
     CDS             8166..9092
                     /gene="dnaI"
                     /locus_tag="IVW65_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7901032.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9221..9661
                     /locus_tag="IVW65_002173"
     CDS             9221..9661
                     /locus_tag="IVW65_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7901033.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         3361 a         1726 c         2121 g         2529 t
ORIGIN      
        1 tttgaaattg taaaagttgc gaaagccgta cgcattgcgt tttaatactt tgatgtggtt
       61 gtttaagcat tccaatggtc cgtttgaata gctgtagtga agcgcatttt ggatctgtgg
      121 cagaaacttt ttaaacgttc gtaaagtggt tgtataggtt tctggcagac cgctaatgtc
      181 tctagttaaa caatcctcaa atagtgactg atctcttttc tttaccgcat aaagaatgtc
      241 ttggtataac tgatagcctt ggcgtaagtt gggacaagca gctaacagtc gatcggcaat
      301 ctcactttca gtcaaataat ctttaaaact ggcacgccaa acccgttttt catagtttaa
      361 ctttgcttct tctttttgta ggagtttcca atagcgcttc aattttttcc ctaaccctcg
      421 ttgagagtgg gaatttcctt taagtcgtac cgtggtttct ttcactcgat gattcaagaa
      481 agttctgcca atatgttgga cgatgtggaa acgatcgatg atcaactgag cattcgggaa
      541 aagctttttc accaacgaaa cataaggcgc atacatatca ataacaatca attgaacggc
      601 ttcacgagtc gctaatgaaa accgagaaaa atagcgttct aaataaggca agcgtcgatt
      661 ttcgacaata tccaataatg catggctttg tccgtccatc atgataaaac tcattgcact
      721 ggcaacactt cgaaccgatt tgaattcatc aaagcataag acctttggca aagtctgttt
      781 gaaaggattc aatgggcggt agaatcgctt catgacacga tagaccgaag aagtggaaat
      841 atttttctga cgagcgattt ccgtcatgga agtattctct tttaagcgag caatgacaga
      901 ccaacgaact ttttgactaa tgaaacagtg tttttcagca acagaggtat ccgctacgaa
      961 tgttctttgg caagacttac atttaaatcg ttgtttcttc agctccagat aagtgcgata
     1021 ctcagacaca tcattcagta acaaccgaac ggttgtcgtc ccccaacgaa tgatcctaga
     1081 ggggacgaca caaagacaat gggaacactc ttctggcttg tacagaagag ttcctttgta
     1141 aatcaagcaa atttgccctt tgtatttttc tttagttaaa caattttcct cgaaatagat
     1201 atttaggtct agaatattta gggtatcttt gataagctga gtatgagaca tcttgaatcc
     1261 tcctatgaat tggtagccaa attaattcta aaggatttca gggtgtcttt tctatatttt
     1321 tttatacaaa aaagatgttg gtgaagttca ccaacatcaa aaagtataga gccattattt
     1381 ggtatggtcc cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa
     1441 ctttacagta aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca
     1501 gtgtggcttt tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa
     1561 acggtcttca tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa
     1621 aagagcaacc gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag
     1681 aaatgtatgc ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa
     1741 tgccatatat acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt
     1801 atgaagcaga tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg
     1861 tcgtcgtgct ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg
     1921 atatcactgt aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa
     1981 aatatgatgg gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct
     2041 ctgacaatat ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac
     2101 aatatggctc agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga
     2161 aagaaaatct gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca
     2221 atacttcagc gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg
     2281 ataaactcgt tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata
     2341 tcgtagaaga agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag
     2401 aggaaatgtt tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata
     2461 cagaggaaat cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc
     2521 tatctgtatt cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa
     2581 aagtatatga tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag
     2641 gaatcgattt tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag
     2701 atatcgaagg aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat
     2761 acggcaaagg ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag
     2821 cgcgaaaagt agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagata
     2881 aaaaccaaga agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga
     2941 tggaaataag cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct
     3001 ctgctcgctt aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt
     3061 tgaattcacc aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca
     3121 aaaaaacaaa aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag
     3181 caccaatcgt agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg
     3241 tcgagggact gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga
     3301 cattgaccca aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc
     3361 gattagatga agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga
     3421 tttattcttc tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg
     3481 aacatttgaa agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg
     3541 tatttggaat cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag
     3601 tgaattttgg tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca
     3661 gccgaaaagc agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag
     3721 aatatatgga aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc
     3781 accgccgtcg atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg
     3841 aacgaacagc tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga
     3901 tgatcgaatt ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag
     3961 ttcatgatga acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg
     4021 tgaaggaagt tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt
     4081 ggggaaaaac gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag
     4141 gtgaaaagaa aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg
     4201 gttagtagta ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca
     4261 accagagaca cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg
     4321 tagagggaag tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat
     4381 ggaaggaaaa tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact
     4441 tttctttttt gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat
     4501 gacaatcgtc gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga
     4561 gcctttgcca gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa
     4621 agcaatcaag ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt
     4681 agatgaagca ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc
     4741 caaagaggta gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc
     4801 cggaggtacc acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt
     4861 agcactgcat gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt
     4921 gaaaacaaaa gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat
     4981 cgcaaagtag gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca
     5041 gccaaagtct ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa
     5101 actgttgagc ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta
     5161 ttgcagcctg atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag
     5221 gaaaaacggc aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat
     5281 caaatcgcct ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac
     5341 gaaggtcatt atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact
     5401 cagcttcagc gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt
     5461 agtagtcaac ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat
     5521 ggaacaaaag aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt
     5581 tgagtatctg tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga
     5641 tcattaaaaa atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg
     5701 ctcaataaag aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa
     5761 gaaaagcagt accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg
     5821 aagcggcaag atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg
     5881 ctcccagaaa gtgaccacat ttccgatgag caaaaaatcg aggaacagcg aatgaaacac
     5941 tatacaggaa tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag
     6001 atgagaaggt gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg
     6061 taagttattt ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca
     6121 ggggacacaa ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta
     6181 ggacagtcgc gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac
     6241 atcctttata taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac
     6301 ataataattc acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc
     6361 gtcgcgaatg tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc
     6421 cgttattagt aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa
     6481 gaggactcat ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg
     6541 tcaaccatgt cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa
     6601 ttggtgaata tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg
     6661 caagtgttta tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg
     6721 tggataaagc aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa
     6781 ggaggcattt ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga
     6841 agaaaagcgg ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca
     6901 ctaatcggcg gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa
     6961 tctggcaaag gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc
     7021 ccgcaatttt atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt
     7081 aaagaagata gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct
     7141 aaagcatttt tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga
     7201 cgattccaga agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa
     7261 aaaatcacaa aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat
     7321 caagaaaaca tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt
     7381 gaagcagatt cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt
     7441 gcaagtcttg atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc
     7501 gatgaattga cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta
     7561 tctgaaaaag aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca
     7621 aaagaaacag ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca
     7681 aacgcagcgc ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa
     7741 gcaatcaagt cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat
     7801 cttgtttctc aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta
     7861 gtgatcaaga ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg
     7921 gcacaaaaga aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat
     7981 caagcaaaac aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt
     8041 cgacgggaaa agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc
     8101 gagaaaaaag cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat
     8161 atctagtgga agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag
     8221 aaaagtatgc tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa
     8281 gcgcacacca agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg
     8341 aattcgtgca agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct
     8401 atgagccgaa gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag
     8461 cattgttagc acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc
     8521 caaaagatat tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg
     8581 cgagcttaga agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata
     8641 aaggactgta tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag
     8701 cgagagaatt agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg
     8761 ttgagatgaa acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac
     8821 gttcacctat tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag
     8881 acgaagtatt cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct
     8941 catcgaattt tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg
     9001 aagagccgct aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg
     9061 aaatgactgg tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga
     9121 cctagtagta aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca
     9181 ccgcaacaag taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag
     9241 tatatctgta gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac
     9301 aggttctgtt gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga
     9361 gagacaaagc atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga
     9421 aacgcttcag ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac
     9481 gtcattcagc aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat
     9541 aaatgaattg aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac
     9601 gatgtctatc ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata
     9661 accaataaca ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt
     9721 tttaaggaga tatggga
//
LOCUS       DACCWK010000094         9734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCWK010000094 DACCWK010000000
VERSION     DACCWK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.097"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..847)
                     /locus_tag="IVW65_002174"
     CDS             complement(<1..847)
                     /locus_tag="IVW65_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7901034.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDFFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYN"
     gene            complement(819..1628)
                     /locus_tag="IVW65_002175"
     CDS             complement(819..1628)
                     /locus_tag="IVW65_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7901035.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            complement(1687..3078)
                     /locus_tag="IVW65_002176"
     CDS             complement(1687..3078)
                     /locus_tag="IVW65_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7901036.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            complement(3366..5504)
                     /locus_tag="IVW65_002177"
     CDS             complement(3366..5504)
                     /locus_tag="IVW65_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7901037.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(5543..7129)
                     /locus_tag="IVW65_002178"
     CDS             complement(5543..7129)
                     /locus_tag="IVW65_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7901038.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(7119..8339)
                     /locus_tag="IVW65_002179"
     CDS             complement(7119..8339)
                     /locus_tag="IVW65_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7901039.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSNGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(8351..9157)
                     /locus_tag="IVW65_002180"
     CDS             complement(8351..9157)
                     /locus_tag="IVW65_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7901040.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            9465..>9734
                     /locus_tag="IVW65_002181"
     CDS             9465..>9734
                     /locus_tag="IVW65_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7901041.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCG"
BASE COUNT         2940 a         1955 c         1503 g         3336 t
ORIGIN      
        1 gattataata tttttgcttt tctttctgat tttgattctt gccagtaatt ttaaaccctg
       61 ttgaaaggta tccgtctact aatcgagcat tggccaaatt aataagtcga ggataatctt
      121 tctttacttc atcaactaat atttttcttt gcaaaatatt atcatactgt tttgaattaa
      181 aagactgata aacgatgcta tcattatttt gataataaaa aatacctgaa aatggcaaaa
      241 tatcaatttt actagccgct ctaaaagctt catacatagg aaccatatcc tcatataatt
      301 tattttctgg aaaaactata tgattgaata attccctttt aaaaaattta ttccatacgt
      361 aaaaatccac tgccttactt ctaaacattt gctcaattgc ttcttcactt gaacaaccaa
      421 tcttcttttc gggaacaaat aaatttgcat tttctatatt ttttccttta cttaatccaa
      481 aagcaatgat atcggggtaa ttcctatggt agtctttcaa aacattatta aaacaatcag
      541 gtaaaaaaaa atcgtcacta tctaaaaatc ctaccaaatt tccagttgct tcttttatcc
      601 cacaattcct agcaactgat ggaccagcgt tttccaagtg ataacatgct actttactct
      661 ctagattagc aatttcgtca ccaatgtcac tactaccatc tgtagatcca tcatcaatta
      721 acaagatttc aatattggga taaccttgcg ttaagacgct gtctacagct ctttttaaag
      781 tattttttgc attgtaaaaa ggtatgatta tagaaagttt agtttgtttt tctaagtttt
      841 gattcatata cttttcccct aattttattc ggtaataaac taattacaag atgtccgcaa
      901 ataactatca gaaaatcttt tagtgatcct agaccatctt tccatacgcg atatcggcct
      961 ttaattcctg gtaaaagata tttacttcct ccacgtctag catacatgtc agcacctgct
     1021 ctagcatgga ctagaatctc ttggatatta taccctttat atcccgcctt caacagtctt
     1081 acccacaaat aataatcttc aaatccaatt aatgggcgat agtttcccac ttctaaaata
     1141 gcatttttat caaacatgac tgtcatatga ttaaagggat ttcttctttt cgaaaacaca
     1201 cggatttcat cattatattc aggtacaatc cttcttccga taacattgct aagcgtgcca
     1261 gtaaattcct caatattact cccaacaatc gctatttctg gatctttttg gaagatctct
     1321 aattgttttt ctaatcgttg gggttccatg atatcatctg tatccattct ggctactaag
     1381 cgatttttcg tatgttctaa tccaattgct aaagcaatcc ccaatccttg atttttttct
     1441 aattttacaa ctgtaaacaa gtgaggatct ttttgttgga aataagtgac aactacttga
     1501 agtttttcat ttatagggcc gtcataaact aatacaattt cttttggcat tactgtttgc
     1561 tcaaaaatac tagttaatgc ttcctttaaa tactctggtt tttctttatc atataaagaa
     1621 agcagcatac ttatattttt attttccaat gttctctcct acttctttgc tcaaaatttt
     1681 tcccatctag cgataaattt tctcttgtga aaagtcgatt tctgctaacg aacggatatt
     1741 ttcatcttca tctagtcctt gtgaggacat cttatcaaat aagattttga ttgtttgaag
     1801 taaaatcttc aaatctaata gtaaggaata ctttttgata tacaacaaat caaaacgtag
     1861 tttactatta taatctgata catattttcc gtatacttgt gcatatccgg taatccctgc
     1921 acgtacattg tgtcttaaat aataataagg attttcttca ttgaactgat caacgaaaaa
     1981 tggtctttct ggacgtggtc cgacgatcga catatcgcct ttcaatacat taaagatttg
     2041 aggtaattcg tcaaaacgta cggcacggat aaatttgcct actggtgtca cacgtgcatc
     2101 attgctcttt gccaagaccg gtcctgattt tgcttcagct gttacactca tggaacgaaa
     2161 tttatagatt gaaaattcct tttgattttt cgtaatacgt acttgtttat aaataattgg
     2221 tccaggtgaa gttgttttta ccaaaatcgc tgtgattagc ataaaaggtg aagcaagaat
     2281 cagtaaaata agcgaaacga tgatatcaaa aagacgtttg aataattcat attccggctg
     2341 aattttgaaa ccggaagctt caatgatact ttcatcttcg aaattcatga tgttcggatt
     2401 gatcatcgtt aaattttcaa aagttgtatt taaaaatagc ttcttctctt ttttagttac
     2461 taattgataa atattgattt tttcgtcttc tgggatttta gaagccaagt agacaatatc
     2521 gatatcatca atcaattttt ttgtgttttc gtaatacttg tcgatcacta ctgcttcaac
     2581 tttatgtttg ttgtttttct tagattgaaa gttttggata gctggtgcga catctttctc
     2641 caaacctacg ataaggactt tcttatcacg actgactctt aagtatagtt tataaacaac
     2701 tatccgataa acgctaagta atatcgtgcc aagtataaac gagagcacga tgacgccacg
     2761 cggaaatgca aaccaacgtc cagcaaaagt aactaccatg atccccaatg ctatcagcac
     2821 ctgtccaata acagtaatga agacgatatc actgatcact cggctataga atacataagc
     2881 tcctaacaaa atattcaacg caataaagat aatggaaata aaaactgctg aatgctgaaa
     2941 ggtctctaag tttcttgctg ggacttctcc ttggaacttc agcaaaaagc taagatagat
     3001 cgacacattc aatacaagta catccatgat gataatgaat atcctacgtg ccgcattcca
     3061 ctccccattt ttattcatat atacactcct aaccataaaa taatgcctct atcatcttac
     3121 catagcaaag tttaaaaacc tagtgaataa ccaaattgta aaataaatta aaagaaacta
     3181 gaaaaggtga ctattgtatg tttatatgta tatttctgcc taacagagag ttggacaaaa
     3241 atcgtatcac attcttccta ctgaatgggt gttcttccta tcatctcttc ggtcacaagt
     3301 tacaattttc atatactaaa aaaactgtat tttcatcaat aaaaatgaaa atacagttct
     3361 tttaatcaat caggtctcaa tgaaaaatcg ccgattcctt ctttagttag attatcgtta
     3421 taaaatgggt catgagcaat ataagaaggc cacttatctt gcattttttt tatttccccg
     3481 gcaaaacgtc tttgtttttc tggtgtatct tcataacctc ttgatttaga ttcgaaatga
     3541 tacaattctg cttgatgtgc atagacattg taacgaccca gttcataaac tttcaaacac
     3601 aagtcaacat cattgaatgc tacttctaat gtttcatcaa atccattgac agcattgaag
     3661 tcagctgcct tgaccatcat acaagctgca gtgactgcta agtaattgac gtcaatgacc
     3721 aatcggccga aataaccaca atgtgttcga tcatagttat tcaatgcatg tcctgcaaca
     3781 ccaccgattc ctaataatac tcctgcatgc tgcgttgtat catctggata aaacagtttt
     3841 gctcctacac agccgattcg atcaaactgt gcataagaaa ccatcgctgt catccagtca
     3901 ggttcaatca cttctgtatc gttattcaag aataaaaagt atttgccgtt tgctttttct
     3961 gctgccagat tgttgatacg tgaataattg aatggaatgt caattaattc aacgataaat
     4021 cggtctttca gctgatgctt gtattctgca aataattcct gcattttagg atctgtactt
     4081 ccattatctg caataatgat ttcataattt ggataagacg ttttttcgat aatcgaatcc
     4141 acacaagttt ttagatcttc atagccattc ttcgttggaa tgatcacgct gaccaaatcc
     4201 tcttgcaaga cgtcataagc aatctcataa aatccagaaa ttctgccagg acgtacgctt
     4261 cctttgatat tccttctaga cagggcatct gtcaatgctt tcactccaga atcatagata
     4321 taacttttgg cttcaccatt acttgcggtt gatcctggga tcgtccgcca atgatacaag
     4381 actcggtcaa tatggtaaat atgatctgca gggatttgtt ctgtcacacg aaggaccaaa
     4441 tcataatctt gagagccttc gtagccttta cggaaaccgc caagcttttt cacaatactt
     4501 gttcgatata cgcctaagtg agagatataa ttatttccca tcaatgtatc gggagaccag
     4561 tctgctttga agtgcggatc aaagcgattg ccatctgcat cgattttatc ttcgtcactg
     4621 tagatcaaat ccagttctgg atgtatgttt agcacttttg ccacttcata taatgcaaat
     4681 ggcggtaatt catcatcatt atctaagagc gctataaatt ctccttctgc catttccaac
     4741 gcagaattag ttgccagact gatatgtcca ttttcttgtc ggaaaacaac tttgattcta
     4801 tcatcctttg cttgatagga ttccagacac tttcggatag ctggatcagt agaagcatca
     4861 tcactgatac atagttccca gtgatcatac gtttgatcca ataccgaatc aatgcatttt
     4921 tcgagccatt tgatttcaac gttatagaca ggcataagta tggaaatcaa tggacggtat
     4981 gcaaaccctt gtgcttcttt cctttgctct ttcagatcag gtttctcgtg acgactgatc
     5041 cactctgcat agtcgatcga tcctggtttc aattccagtt tgacccggcg gattgttttt
     5101 tttacgccat gagtccttgc ataatttatc ccttttttca gtaatctttt ttttctttcc
     5161 catgaagatt caatgccgtc atcataaggg tatttaccat ttagtttcac agctatgcca
     5221 ttttgatgtt ttgccgtttg aaaatctaag atcgcttttc ctctcatttt cccggataaa
     5281 cgtatcttaa aaccgctttg ggtcttaaca tcaagattat ataaatgatt gatatccaat
     5341 cttacgacat gtgttgcttc agcaatcaca ttttcttttt ctacctttat cgtagggatt
     5401 tctttggtca cttcatcaat tgcccagccc acaataagca aatcgccagt ggcccgttca
     5461 cgagtgattt gatcgataaa tacggcaatt tctttttttt gcacgattcg tttactcctt
     5521 atctttataa gttaatcatt atttacattt caatgatcca tgattgacca ttttgagcta
     5581 ctgcttccac taagaatgaa cgttcttgct cgcaaaggat tcgtaattca aatcccgctt
     5641 ctgttccttt tgcgagctgt tctgcttgat tgacttcatc tcgtgaaaca ggaattgctt
     5701 cgaaaaatgg cgattggtca gctgataact tataggacaa tggctggcga tccgaattag
     5761 cgattcccca tccgatgatt tttaatattt ttgtttctgc atcccatatt ttctcatcaa
     5821 tacatataga aagctctttt tcttgtattt ttttcgatgt ttttttctta cgtcgtccta
     5881 gtctatgttt gatcgtccat tcatcacttt ccaacaatgt gcaatagcga tttgtggttg
     5941 tttttaacgt atggtctagt cttgtatttt cctcaagaag atgctgattt tctctttcta
     6001 attcatcatt tttctcagaa agctgctttg tgacttcagc cataggacga attgtttcca
     6061 gcaattcttt cattgttggt gtcaattccc cgataaagcg atagtgacaa tggatagtca
     6121 cttctttccc agaaatatcc gttagagcga actctggtat ttctttccca tcaaacaaat
     6181 aacaatcatt tactgttttg actacagcat ttgaatcgat aaatatgagt ttttctccag
     6241 cagcttctgc actgaattcg ataaagctag gttgctgtgc gaagcgaaac accattcttt
     6301 ctgttgcttc tgacacgggg aaacttagtg tctcattttc ccctgtgaaa ttattgaatg
     6361 gtatttgttg gattgtttca tcttgtttcg tttgctgggt tacttctagt acttttacat
     6421 aattagagtt ttgtggctgt gcaatggaag tttgtactac tggatgtttc atcaatgaga
     6481 aaaagtattg gtagacttca ccaaatggtc gcattttgaa atcatagcgt acagcttccg
     6541 gcaactcttc ataagttgat tttaattctg tatctcctac agccaagtac aagtaatctt
     6601 caatattaat gaataatccg gctttttcaa aaactttttg gaaaccatca tgagtataaa
     6661 aagaattatg ggtttcatct aggagaccat aagaagccca aggcaattga ttattgaaca
     6721 aatcgatcat gacagaatta tgtgctaagt tagggaatgt aatcaggatc tctccttctt
     6781 cattcaaaaa ttctctgact ttcttcaatg tttttccagg atctactaaa tgttctaaaa
     6841 catccgcaaa caagacgtaa tcaaatgtct gtccagcata atagtttgcc cattcaaaac
     6901 tttcgatatc tccataaaat ccatcttggg caaattcgct gacaaaatcg aataattcct
     6961 tatctagctc aacaatgctc acttcacatt gttttgctcc gatcaaatgt ttcgttaatc
     7021 gaccgtttcc gggaccaaac tctaaaactt tacttcctgg tttgatctgt gcggcaattt
     7081 ttcccacact gctttgttca tctaggtcca tttcaaaatc atatttcatt gctatgagtc
     7141 ccttcttccc atttatggtc taagaccacg atgccttctc cgatataaga accgctgaca
     7201 aacaagttca tgtattttgt cttgtatacc aaaggaaccg ttgcattttc ttcaaagacc
     7261 gcaacgtcaa aataatattc accagctaac aaacctaact tttcatactc cagataaaag
     7321 atattctcac cttttttcca agggatcttg ataccatcta gtagagtatt gagtccgcac
     7381 acataaaaat tgtcgactgt acgcagagca attcctaaaa ctggattttt gattgtatcg
     7441 tcttttactt cataggttac tttaacgtat agtttttctc cttgttccag catatccact
     7501 ggatttttat ttttatccaa caattcggct ttcgtcacgt ctacgatatc tggagctgcg
     7561 aagcgttcta cttggttttt atttcgatct actgttttaa ctgatttttt cttcaaaaag
     7621 ttttcgtaat tcgtcgttac ttccatcgta tcaccatatt cgacaacttc tccatttttc
     7681 aaccaatagg aacgatcaca aaaacggcga atcgaactaa tatcatgaga aacgaataaa
     7741 atcgtttttc ccgagttctt aatttctgta aatttttcca tacatttcag ttggaattca
     7801 agatctccta ctgccaatgc ttcatccaca atcaaaatat caggattcac attgatcgct
     7861 acagcaaaag ctagacgaac gaacatccca tttgaatagg tttttacagg ttgataaata
     7921 tggtcgccaa tatcagcaaa ttcaatgata tcatcgacca tttcatcgac ttcttctctt
     7981 gaaaatccta gaaccattcc atttaaatag atattttcat agccagaata ctcaggattg
     8041 aatcctgatc ctagttctag caaagctgag atttttcctt cgatttccat cgtgccggat
     8101 gttggtgtca gtactcccgt aatgattttc aacattgtag attttcccga tccgttttct
     8161 cccacgaaac cgatcatctc accttttttt atttctacat tcacgttttt caacgcataa
     8221 aatacatcat gataggattt cttaaaagga ttgagggctt cacgaaatcg atccgaaggt
     8281 tttgcgtaca tattataaga ttttgtgata tcttttagtc taattgcata ttctgtcata
     8341 gctattctcc ttacagcaca tctgaaaaat gtggtttcaa tctacggaac actgcagagc
     8401 cgattacgaa cattactaac gtgaaaaccc agaaatacaa agtatatgtt ccatgctccc
     8461 agaaccaagc ttcacttaaa aatgactcac gataaccttg aacaacatag tacagcggat
     8521 ttaatttcaa tatgcttaca agacctgaat caaactgatt agggctccat aatatcggta
     8581 atgtccacat gatcaactgc agcacgatcg taattaattg catgatatca ggtaaaaatg
     8641 gttggatcga tgccgtcagc caagtcaatc ctgtcaagaa agcacacatg caaaataaat
     8701 aataaatcag ctgtaaagac attgctgaag gatatatacc atttataagc gcgacagcta
     8761 tgccaatcag aatgaagaat aaatgggtgt acagattgga taggattttc gtcgtgggca
     8821 agatacgaat attgaatacg actttcttta ctaaataagt atattcccga aaaacaaccg
     8881 tagttgatgc aaatgcatct gagaagaaga accagggaat catcccggta actaaatata
     8941 cgataaatgg gtattttgca tcgatcattt gtgctttcag tccaacacca aataccagcc
     9001 aataaatcaa tacagtaaat agcggattga taaacgccca taagattcct aaaaacgatc
     9061 ctgcatatct tgatttgaag tcattgatcg aaaactgtag cagtaatttt ctattctgat
     9121 aaatgtcttt cataaataaa tacaaatctt taaacatttg tgttcctcca agcgatttca
     9181 gtgaattaca gtcaagtgcc attttaccat atttttggta ccgagctgcg ttcttatttc
     9241 tttcttaatt ttctatgata caagattttc tctaacgttt ctattttaca gaagatgcta
     9301 gacaattgca ataaaccaaa aaaggggttc tataatattt tgtgttggtg gaaaatcgaa
     9361 ggcgattttc tactaacact ttttcttcgt ttaaactcaa aaagagactc acttttggta
     9421 aaatttagtc gaccaaaacg tcatcctacc aaaggagtct ctcaatggat aatcatacta
     9481 gaaaattact cggattaaca gataaacatt tattttttga tgaagaatgg ctgattgaaa
     9541 aagaatataa aggcgttcaa gcgcagttta ttaaaggaaa attggatgac tctccttcct
     9601 attgtgaaca ctgtcgatcg gttcgtatta tccgcaacgg ttcctatacg acacgcactc
     9661 agatgttgaa agtcaaagaa aagttaacta ttttagaatt aaaacgtacc cgttttctct
     9721 gccatgattg tgga
//
LOCUS       DACCWK010000095         9699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCWK010000095 DACCWK010000000
VERSION     DACCWK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.098"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            152..1543
                     /locus_tag="IVW65_002182"
     CDS             152..1543
                     /locus_tag="IVW65_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7901042.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1616..2263)
                     /locus_tag="IVW65_002183"
     CDS             complement(1616..2263)
                     /locus_tag="IVW65_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7901043.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2271..2957)
                     /locus_tag="IVW65_002184"
     CDS             complement(2271..2957)
                     /locus_tag="IVW65_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7901044.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2963..4036)
                     /locus_tag="IVW65_002185"
     CDS             complement(2963..4036)
                     /locus_tag="IVW65_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7901045.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4258..4932
                     /locus_tag="IVW65_002186"
     CDS             4258..4932
                     /locus_tag="IVW65_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7901046.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4937..6277
                     /locus_tag="IVW65_002187"
     CDS             4937..6277
                     /locus_tag="IVW65_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7901047.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6426..7412)
                     /locus_tag="IVW65_002188"
     CDS             complement(6426..7412)
                     /locus_tag="IVW65_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7901048.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            8136..9167
                     /locus_tag="IVW65_002189"
     CDS             8136..9167
                     /locus_tag="IVW65_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7901049.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            9276..>9699
                     /locus_tag="IVW65_002190"
     CDS             9276..>9699
                     /locus_tag="IVW65_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7901050.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         3115 a         1755 c         1672 g         3157 t
ORIGIN      
        1 acgaagggcg ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt
       61 tgattccttc gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat
      121 gatcaggttg ttggaaggaa actatagaaa gatggaaaaa atcattcgaa ttctgtccat
      181 ttctgacaag tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac
      241 atttgtaagg tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc
      301 ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct
      361 gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc
      421 gggtaaaacg attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg
      481 taagttgaaa caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca
      541 aggttttcag ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt
      601 ctttttaagt tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa
      661 agaaattatg gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga
      721 gcaatctttc tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc
      781 atttgaatgg aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt
      841 cttaatcttg aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa
      901 ctattttgtc aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga
      961 gcagatacga aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa
     1021 cgaagaatat gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata
     1081 cgatacgact catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca
     1141 gatacgtcta tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca
     1201 gaatctgatt tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat
     1261 tcaccattat acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag
     1321 tcaatatatt gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac
     1381 aactgctatt actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt
     1441 tacgaatcaa cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc
     1501 attcgcaaat gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct
     1561 atgtataaac caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt
     1621 gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg
     1681 tccaattatt tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata
     1741 ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca
     1801 gccatttttc gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat
     1861 catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc
     1921 agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga
     1981 attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat
     2041 cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga
     2101 tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat
     2161 cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct
     2221 ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc
     2281 tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga
     2341 atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta
     2401 actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct
     2461 gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct
     2521 ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc
     2581 aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat
     2641 tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct
     2701 ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc
     2761 actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta
     2821 cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt
     2881 gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc
     2941 gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta
     3001 tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata
     3061 gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca
     3121 aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta
     3181 ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag
     3241 cagtcttctg aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga
     3301 acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt
     3361 caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt
     3421 tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct
     3481 gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg
     3541 tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt
     3601 tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat
     3661 tctttgtttt aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg
     3721 tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga
     3781 ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg
     3841 caaatacttg atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg
     3901 cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg
     3961 caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt
     4021 tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac
     4081 ttgttgaata gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca
     4141 ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta
     4201 agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg
     4261 attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc
     4321 gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa
     4381 agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa
     4441 gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa
     4501 gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa
     4561 cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt
     4621 ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa
     4681 gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat
     4741 tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga
     4801 tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat
     4861 ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg
     4921 caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc
     4981 gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat
     5041 attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc
     5101 tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac
     5161 tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg
     5221 ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat
     5281 tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat
     5341 ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac
     5401 gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa
     5461 tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt
     5521 ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat
     5581 tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc
     5641 gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat
     5701 tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac
     5761 ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat
     5821 gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat
     5881 agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa
     5941 aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga
     6001 agataagatc attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa
     6061 tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga
     6121 agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca
     6181 ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga
     6241 ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg
     6301 tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct
     6361 tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct
     6421 tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc
     6481 aattcttctc ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga
     6541 atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt
     6601 gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt
     6661 actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta
     6721 tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca
     6781 tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg
     6841 tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt
     6901 cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc
     6961 attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca
     7021 gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg
     7081 atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt
     7141 gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa
     7201 gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat accagacaat
     7261 ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt
     7321 tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag
     7381 cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa atagatttat
     7441 gattggtata ttgttactta taaatttatt ctataggaga cgattgtttt ctacaaatat
     7501 ttagattgga atttgattaa aacaatttaa ttgaaatttg aaaacattta tgttaatgaa
     7561 agtcatattc taataaaatg tcatttgttt ctatatgaat ttttatgaaa aatttcatat
     7621 aatttccact cgttgtgatg gtttaattta tccaatccat caataagcaa acaacttata
     7681 taaaaaagga ttaattctca atataattgc tcctaccata gaataaatct tcatgtaaag
     7741 taatgaagta ccaactattt ctaatacttc tttaagaatt atcccaacaa tatcgttaga
     7801 caaaccatta ttgaataaat ggagagccaa tcgattctga aaaaatgttt tctaattttt
     7861 cggaaataaa agaaagagcc tcctgccatt agaacgaacc aaatccattt cattgctcat
     7921 gaagtaacca catagctaaa aagaggataa atcaattaaa agatcaaaag aatgactgca
     7981 ctaataaaat aactaaaaat gtatcttctt acttcatatc ttttagctag tacttagtta
     8041 aaaatttgct tcctaagatc aagacaaact gtctatcttc atttgatgaa atgcggtaaa
     8101 atatagaaaa tgaacactat tggagggaat ttgtaatgga cagcaaaact agaaaacaac
     8161 tgcaagaaaa gttaaagcaa cgtacacact tgctcccttc cgtctttcaa cagtttttag
     8221 aagaaaatcg ttcttccctt tcattaggca gtcgctatga atacgcaaaa gattttcatc
     8281 tgtttactga ttatttgaga aaagtatttt ctgaagaagt ggatgatcag ctgattatcc
     8341 atttagaaaa acaagattat cagaatttct tagctaaaat ctatcgacat actcgttcgt
     8401 tccaaactac agcgaaccaa gaaacggagc aattgtttga aaatagctat tacgggattt
     8461 caaggaagca atattcatta aatcattttt tacgattttt gtatcaaaaa ggactgattg
     8521 aagaagagat ttctcttttg ggtacaagtt taccggaaac aaccaaagca gctccgcgat
     8581 atatcgatgc agaacttttc aaagattttg agcatttgtt tatcccgatt gaaggctttc
     8641 aaacaagcag agaagctagc tttgaagaaa agaatagacc gaggaacctt gcaattactg
     8701 ccattcttct gtattgcgga ttaaagatcc atgaagtagt agaacttaaa cgtaaagatg
     8761 tatctttgac caagcagatt cttaatctga atagaaaaga aaacagactg aataaagtac
     8821 caataccaaa agaagcgatg ccatttctgg agaactattt taagttaaca gcacaaaata
     8881 cggacgaaaa ttcttttgtt tttcgttcct cacatgatca gcaaatttct cctcggacta
     8941 ttcgccaaat tttgagcaaa attacagtat ataaaaacgt atcgccagaa ctatgcagaa
     9001 aaacatatcg ttattatgca gaactatatt tagatgatgc tgacgcggta aattatttat
     9061 gtggtaatcg atacctctca tcccattctt ttcaagatat ctgggaaaag atggttgatt
     9121 tttcttatca cgaattagcg gcattcgttc gggtttcttc catataaaac aaatgtatga
     9181 tctttttttc acaataaact atgttattat ataaaggatt atgttacgat tatcttgaat
     9241 ttgtatatta cttaacaaaa gataatggag tgtgaatgat ggagaccata acgtattccc
     9301 ctttgaactt atttacgaat tttcaagaag ctgcagctaa cttttccgag accccgatta
     9361 tctttgatga gccactttca gctttcccag aattaggact tgaaacaact tacaaaaaat
     9421 gctgcgaaag tgtgttgaaa cgggcttatc aattggctca tcttggagtg aaatccggag
     9481 acaaagttat tatttataag agttccgctt ttgacacata ccttctagct gtttctgtgt
     9541 cttaccttgg tgcagtgcct gtcatgactt cttatcatct accaacaaca acaatggaag
     9601 tatttatcga tcgtttagaa gaccctttca ttttatttga tgatgagaca aaaaagcgag
     9661 tgagagaaat ttcaaatgga acaaaatcaa aacagattc
//
LOCUS       DACCWK010000096         9525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCWK010000096 DACCWK010000000
VERSION     DACCWK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.099"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            31..225
                     /locus_tag="IVW65_002191"
     CDS             31..225
                     /locus_tag="IVW65_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901051.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            494..793
                     /locus_tag="IVW65_002192"
     CDS             494..793
                     /locus_tag="IVW65_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901052.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(890..1324)
                     /locus_tag="IVW65_002193"
     CDS             complement(890..1324)
                     /locus_tag="IVW65_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7901053.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1556..2266)
                     /locus_tag="IVW65_002194"
     CDS             complement(1556..2266)
                     /locus_tag="IVW65_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7901054.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2256..3116)
                     /locus_tag="IVW65_002195"
     CDS             complement(2256..3116)
                     /locus_tag="IVW65_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7901055.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3121..3759)
                     /locus_tag="IVW65_002196"
     CDS             complement(3121..3759)
                     /locus_tag="IVW65_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7901056.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3774..4073)
                     /locus_tag="IVW65_002197"
     CDS             complement(3774..4073)
                     /locus_tag="IVW65_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7901057.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4116..5600)
                     /locus_tag="IVW65_002198"
     CDS             complement(4116..5600)
                     /locus_tag="IVW65_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7901058.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5620..6081)
                     /locus_tag="IVW65_002199"
     CDS             complement(5620..6081)
                     /locus_tag="IVW65_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7901059.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6099..7166)
                     /gene="ulaG"
                     /locus_tag="IVW65_002200"
     CDS             complement(6099..7166)
                     /gene="ulaG"
                     /locus_tag="IVW65_002200"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7901060.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7384..8154
                     /locus_tag="IVW65_002201"
     CDS             7384..8154
                     /locus_tag="IVW65_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7901061.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8427..9254)
                     /locus_tag="IVW65_002202"
     CDS             complement(8427..9254)
                     /locus_tag="IVW65_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7901062.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2965 a         1947 c         1497 g         3116 t
ORIGIN      
        1 actaaaaaca gcaagaaaga agaggatcaa atgtattatg tagaagtaaa aacaaaaggc
       61 gtcaaaaaca aacaatatgt gaaaggtatc agtaacgaat atcctttact tggttcatgg
      121 aaagaagctg ctcctttttc caaaccatgt gccataaaaa taaagaacga gctagaaaaa
      181 gaactgactt gtggtaaagc tgttgtagat atcatcgaaa agtaaatcca atgtatagaa
      241 aggaagatca cacatgaaaa gcgacgtaca aattgctaag attatagaat tagaagaaga
      301 agacgcagtg ttgacaattg aaaaaaaata aatgtttgta taccatatga ttatttcaat
      361 atgtatcctg gaattggaga tctcgtagag ctatatcaag atgaatcaaa tatacttgtc
      421 attcatcatg aaatatagag acttataaaa ttgtttattt ttataatgta tcagtataga
      481 aaggaaataa aacatggatt atactgttga atttgacagt gaactactta agcaatttcc
      541 acaagaagaa catttcatgt ataaagaagc aatttcccac aaagtgacag agtatttgga
      601 gcataatttt tttcgtataa agccagtgcg tactcgttcg gcaaaaggaa tatacgagat
      661 gaaagtcaaa attaataaaa aaaattatcg catagcattt gctttaaaaa aaaatcatgt
      721 gttcattttt tatattagtt cgaatttgca aaaaattgta tttgataaag aggtgagtaa
      781 aaaaataaaa tagctgagca ttggaggata gattgagtga tactaagtct atcctcttgt
      841 ttttctaaag aactaagcta gaattttttc ttttgtacgt tattatatgt tatttgtcat
      901 taccaaaaag cacctttttt cttctagtat ctatgacata agaaatacca cccatttcct
      961 ttacttgaac aaatcctact gattcataaa atttttttgt tttctcagta tcatctgtca
     1021 ataattcgat ttggactggt ccataactag tgatcttctc caaagcttca ttcaaaagca
     1081 ctttgcctat tccttggcgc tgatacctcg gatgaatgag gatatcttga ataaatgcga
     1141 tcgtcgcaaa atcagtgatc caacgaacga ttccgataat cttcccttcc ccattacgct
     1201 ttatcagaga ttcagattgt tcaaatgctc tagctaacct tgcagtgtct tttgtgtaat
     1261 tcgtccaacc aacgctctta tataattcag aaatctcatc gatcataagg gaatcaatat
     1321 tcattttttt cttcctttct tatacaattc tccctctaat aatacagttc tttgactatt
     1381 taatctatca catataactc tcctgtaaag attctctcga ttccttaaaa ttcctctttc
     1441 cttttagaat gcatactttt tgataaataa aaaggttgtg ataaattttc ttcacaacct
     1501 ctgtttctct ctatcaagtg ttatctttta attttctacc cttaccctag catctctatt
     1561 gcccgtagta tgcatacttt ccatgtttgc gaaaataatg tttatctagt aaatattgcg
     1621 ggatttcaca tatattgggg ttgatcagct cgttttcttt ggccatcaga caaatttcat
     1681 ctaagatcaa actgttttct actgctttca taggtgaatc cccccatgtg aacggtccat
     1741 gtccatagac aagtactcca gggacttcgt ttggatctaa ttttctttcc ttaaacgtct
     1801 cgacaatcac attccctgta tgaacttcgt aagcttctct tacttcttct ttcgttaatt
     1861 ggcgtgtaca aggaacctta ccataaaaag catcagcatg agtcgtgcca taagctggca
     1921 aatcgcgtcc tgcttgcgcc cacatgacgg agtgcgtgga atgagtatga gtaatagcag
     1981 ttatatcttc aaaagtttga tacaaaacaa catgtgtggg gagatctgaa gatggtttta
     2041 agctatcttc ttctattata tttcccgaca aatctgttac caccatttga tctgcttgca
     2101 tgcattcata tctgacaccg cttggcttaa tgacgatgac tcctagctca cggtccactt
     2161 cactcacatt gccccaagtc aatttgacta atccgtatcg cggcaatgcc agatttgctt
     2221 caaggacacg ttgcttcatt gctgcaattt tttcttcaca agacatcata gccagcctcc
     2281 ttcaatttag aacttaagta gtcttttgcc tgctggattt ctcttcgaaa gtcttggctt
     2341 ttttcactcc acatttcaat cagaaatgtt ccatcataat ctagacgttt caaatgtctt
     2401 aataatccaa gaaaatcaac acaaccgcta ccaaaaggaa catcacgaaa ctggcctgaa
     2461 tattcttttg ttacagggta agtatctttt aagtggatag cagtgatata atcgatccct
     2521 ttctcaagtt ctcgagccgg attattttcc ggccatgcgg acaaattgcc taggtctgga
     2581 tatacttgta gaaaaggtga atgaatctgt tctttgattt ctaaaaattt tgaaatggag
     2641 ttcataaatg gatcatccat gatttccacg gaaagaatga tgccatattt agaagcttcc
     2701 tttacgcttt cttttattcc ttcgataaag tcttctctgg aattcattga tttctcttca
     2761 taataaacat cgtatccagc tatttggatt actttgacgc ttagattatg cgccaaatgg
     2821 attgcttttt tcattagttg tttcgctgct tttttctttt ctggatcagc agaaccaaac
     2881 ggaaatcgcc gatgcgcact taaacaaatc gaatggatac gtacaccgga gctgaacatc
     2941 tcttctctca gctcagctat cttttcttca gaccaatcta atcgtgctaa ccgctcatcc
     3001 gtctcatcaa tcgacatctc gataaaatcg aagtccattt cttttacaag ggaaaaacgt
     3061 tctttccatg aaatatcttt gggtaacgct ttttcataaa tcccaatttg agccatttcc
     3121 ttacccccag atacgaatga tctcatcttt gaagttttgt gcggccttca tcggatcatt
     3181 tgctgctgta ataccacgac cagtgataaa ggtatacaca tcgatccctt caaataagtg
     3241 taatgtgtgt ggatttaatc caccagttac agaaacatta aagcctagtt caatcagttt
     3301 tttgatcttc gataaatctt tttcacccca agtttcacca gataacagcg catcacggct
     3361 ttgatggtag attacctgcc ggataccgat gttatgccaa tccatggctt gttcaaaagt
     3421 ccaattgccg tagagttcta cttgcaattc acctatttct ttttgtgctg cctccatagt
     3481 tggaatcgtt gcacaacaaa tgactgtcat ccagtctgct cctgcttttg caacattact
     3541 cgccactgtt cctccagcgt ctgcacattt cgtatcagcg actagttttt tatctgggaa
     3601 catactgcga atacagcgaa tcgctttttg tccttcctgt aagcaaagaa ttgtgcccac
     3661 ttcaataata tctactacat caccaacttt cataacatct cctaatgcat gttctaagtc
     3721 attgtgatct aacgcaattt gcaaattcgg cctgctcatc ttagttcctt cctttacttg
     3781 tttttttggt accactttgt ttcagcaaat ttttcactga tttctttgac tgacatcaca
     3841 tttttgatac cgataactgt aactcctttt ttctctgcct gagtaaacat attcaaaaaa
     3901 ttgactggcg taaaaactac atcgtattgg ccagctgaac tttttccctc tgaaatggca
     3961 caatgatgga ttttggtaat tggtacgttt agttctttca ttgctttttc cacacttctc
     4021 atcatcatca agcttgttcc tgatccattt gcacacgata ctaaaattct catcttaatc
     4081 cctccattat gagtgttgtc taatcagatt ttcttttata ctctctgctt ttctgcatat
     4141 gtgtcatagt cttctacaat caaaaaataa ccatcaggat ttttccgata ttgtaactgc
     4201 ggaatagcca ataaaccaat gacaactacg gcaattccag cataacctag gactttcatg
     4261 attactgtaa acaatggcca agctgttgcc cagtcgaaca tcccgatata accaccgtat
     4321 gttgacatcc caatccatgt agcgaataat gctgaaccgc ctacttgtat gattcctgaa
     4381 agaaacggga acaggcaagc ggctttgaat ccacctcggt tattggcata aactgctata
     4441 acggcattat caaagaacaa cggaatgaaa ccggctatga cgatcgttgg tgatttcaat
     4501 aaaatcagta aaccaatcat caaaaactgt cccaaagcac caaacaagaa cccaatcgtt
     4561 accgcatttg gcgaaccaaa gccaaaagta gcagctacgt caattcctgg aacagctcct
     4621 ggtaaaatcg tgtttgagat tccttggaaa gattgagtta gttcatcgac gaatgtccga
     4681 acacctaatt gcaagatcgc cagatatacc gcaaaattta aagaagtcgt cataatgtag
     4741 aataagaaac tctgcccttc ttgcatgaat tgcgcttgaa tcaaataatc tttgcctagt
     4801 acaatcagaa tgatcccaaa gaaaaatagc atgagtaacg aagtagcaac catattctca
     4861 ttgaaaatcg acaagaatcc tggtaatttg atatcttcaa tttttttgtc ttgcttgatt
     4921 gatctatttt tcttttcatc ccgctttttc atccactcag ctaattttgc aaatataaag
     4981 attccgaaca tttgctgatg tgccactgca aatccggcac cttctgtcaa ttcctgcgtg
     5041 attcctactg ttaagttaga agctactgcc caataacaac ctaaaatcag tcccataaaa
     5101 atcaatactt gtatcctacc tagatcagga aagcaaaaca gtaaaatcca aaaagcggta
     5161 gctgcttgtt gaacctgaac gtttccggta gtgaaaacgg aacgcagttt cgtatatttc
     5221 tgaaaacgga ctaataagat atttaaaaca aatgcaatca gtaataaaat catcacatct
     5281 ccaaaggtac ggccaaatgt ctccattaat ccagcatcca cagcattttg tccaaaataa
     5341 ggatctgtaa ccatggcatc caaattgaac cgttccttta gtccaaccaa gattggccga
     5401 aaattattga ccaaaccacc agagccaaca gtcaaaataa agtaaccgac cgttgccttt
     5461 aagaatccag cgatgcattc atgaagcggg cgtttcagca aaatatagcc taataacacg
     5521 ataaaaccaa tcaggtatgc tggctgcgtc aagatatttg ttgcaaaata cgtccaaatg
     5581 cttaacaaaa attcccccat cttttttctc ctctatcaat taattatatt ctgccatcac
     5641 aacttggtaa tcatcgatgg agttcgtaga gatgagacgc tcaaccaatc catctgtcat
     5701 taataattcg gataattttt ggatattttc tagatgttct tctggatctt ttgctgcgag
     5761 tgtaaaaaac aaactagccg ttttctcctc attctcccct tcactttcaa aacggattgg
     5821 ctgtttcaac ttggtaaatg aaatagctgt tccaaaaaca ccatcacttt tttctgaaga
     5881 atgcggcatt gctacttgag gtgcgatcac aatataagga ccgtattttt ctacacaatg
     5941 gatgatctcc tccacatacg tttcatcaat aatttcctta tcgatcaata caagacagct
     6001 ttcggcgata gcttcacgcc aatccgtgat ttctttatct gggtaatgaa tcaattgatg
     6061 gtcaaaaaag tatttcagca agaaactccc cttctttctt ataaaaacga cggaaacggt
     6121 gtatcatttt caatcgagaa gacatcttca aatcctcgat aaaaattaaa ctcaagatca
     6181 tctttgtttg tcggatagat gaatttcccg cctacttgcc agatataagg tttaaatcca
     6241 tacttcaggc gatccttttt gaatttccaa atttcagtga tctcttttgg atcagccata
     6301 aaattagacc aaatatcgta gtggacagga atcactactt ttgcattcaa tgactccgcc
     6361 atgcgcagca tatcaacaga agtaactttg tctgttatcc ctcgtgggtt ttcaccatag
     6421 gcccctaaac agacatcgat tttgtgttca ttaccatgtt tggcaaataa attggagtaa
     6481 tgtgaatctc ctgcatgata aagattccct cctgatgttt cgaataaata gtttactgca
     6541 atttgatcca tctcctgagg catttttcct tttaatacct cttctggatc ttcacaagtg
     6601 accaacgctg tccgatcaaa cgcttctaaa gctactacat tgatgtcttt gattttgact
     6661 gcatcacctg gctttactgt aatcgttttt tcttcaggaa ttccccattt ttgccacgtg
     6721 cgaaccactt cttttggccc aatgaatgtt gcttcaggac aattttgatg aactgctgcg
     6781 gctgtatgga tatctagatg atcggaatgg atatgtgtaa cgattaaagc gtctacattt
     6841 tttatcgcaa aaggatcgat tacgaaaggc tgagtacgta aattaggctg catattttga
     6901 acgccactca tacgcatcat ctgatggcct tttttcatca tgccattccc atgactacgt
     6961 tttcccgtcc cacaccatag atcacataaa atattcgttc cttcatgact tttcagccaa
     7021 attcccgtac agcccagcca ccacatagca aaagttccag gatccactac ttctgtttcg
     7081 atttcttcat tcaagtatgt gccccactca ggaaatgttt ctactaacca cttttctttt
     7141 gttacttcat tgacagtttt actcatttgc ttgctccccc catatcgttg ttccaactta
     7201 caagtaaata ttaatgtgta tcagcatcca tgaaaaccct tttatatgac cgtttgtttt
     7261 ttatatgatt ttctttcagt aatcaatgat tgtttcagta taataaagac agatatgaat
     7321 gtttaaatga acaactagtc aaataaatcc atggatcaag atgtccctca ggaggcaaaa
     7381 gtcatgaaaa actcacttgt caatattgaa aaacgtcaaa aagaaatttt ggaactgctt
     7441 cagcagactg aacaactatc aactttcgaa ttagctgaat cccttgatgt atctattagt
     7501 acgatacgaa gagatttaaa tttattagaa gaaaaaaacg agatcatacg caattacggt
     7561 tattgttctt ataattacaa aaaccagact gattttgatc aatctggtcc tgaaaggatt
     7621 aaacaagcga ttgccagaga agctagtaaa tacattaacg attatgatac gctgtttatc
     7681 aattctagct cgactgcgtt gaaaacactg gattttcttc aatctaacca tttgacagtc
     7741 attacaaaca acgtgaaaat caattactct ccgcacaaag ataattgtag ttatatttta
     7801 actggtggag agatgcgctt tccaaaagaa gcactagtgg gcgacatcgc attaaatacg
     7861 atttcttcag tcaatgcaga catttgtatc attggctgta gcggagtcga tgtagtacat
     7921 ggggtcacaa caaagatatt aaatgaatca acgatcaacg aaatgatggt caacaagaca
     7981 atcaaatgta agattttagt cgctgatcat cgaaaaatcg gactgacatc aaagttcaag
     8041 attgctgata tttcagtctt tgattatttg atcactgata aattttgttc tgaaaaagta
     8101 ttagaggaga tcagaaatac ggggataaaa accattcaag tagattcatt ttaagaacca
     8161 ggacgtaaat atgtaaatag gattatcaaa aaaatgaata acaaatttat cgataaatga
     8221 aaaacacgct acgctcaaat tgataaagga atcccaagat gtatccatcc aatcgatacc
     8281 atcataatct attgcttctt ttataaccgt tatatgttgc ctaaatagga atcaaacaat
     8341 atcgaaaaaa ggaggtatga caaaagtcac acctcctttt ttcgaataaa cggtattcaa
     8401 atcagaacac tgatttcaca accactttat ttcaggtcgt tttcaatcac ctttaccgcg
     8461 taatcatttc cgttgaaacg cgctattttt tttgcgtcca tatatctttc taaataatca
     8521 tcttctccta attgcttatg gcaagaagca atttcataaa gcaagtattc caatgcataa
     8581 gtcgatttat gtttcttatt atactcaata cccttctcac ataattggat tgctttatca
     8641 taatcctcga ttttacggta ataggtggcg atatcataat atacaatgca gaccatcaga
     8701 ttatcaaaat cagtctcact caatatttta agtgtacgct ccacataaac tttcgcaaaa
     8761 tctaattctc cttttgacaa gtagatcatt gccacgtttg cagctgcttg taagtgatgg
     8821 atatctgttt cggtcgtttg ttccaacact tgattaaaat aaaataatgc tgcatctggt
     8881 tttttcgcat aaagatgatc aagcagtcct tgataaaata aaatttgatt taaaagatca
     8941 gaattactta ctttttcctg atcgatcgtt tgtaaaattt tataagattc ttggtatttt
     9001 gatacagaat tcaatgattg aatctcattt atttttttcc tgattttgtc ttcttctgtt
     9061 tctactaata tatcggagat ctcgacattc agctttttgc aaagcgcact taagatattg
     9121 atgcctggcg acatattctt gttttcgata ttgctgacag ttgcttgttc acaaatgcct
     9181 tctgctaatt gtacttgtgt taaccccttt ttctttcggt attcttttag taatgctcca
     9241 tctactttaa tcaaaacaat ttccttccct ttaaaaaatt atttctcctt tatatcatac
     9301 acaaaaaaaa gttctaaaca accaaaaatg tttagaacag aataaaaatt ttaattttaa
     9361 aataatattc aaattaaaca actggcgtat ctgctccttg atttcttttt gtgttatctg
     9421 aataattatc gtttacagtt ataacacata gtgaaaattg tcatggcgag aaagtctaag
     9481 aatattttta aacataacta tttctttctt acgtgtgtat aatga
//
LOCUS       DACCWK010000097         9436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCWK010000097 DACCWK010000000
VERSION     DACCWK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.100"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            77..1381
                     /locus_tag="IVW65_002203"
     CDS             77..1381
                     /locus_tag="IVW65_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7901063.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1473..1838)
                     /locus_tag="IVW65_002204"
     CDS             complement(1473..1838)
                     /locus_tag="IVW65_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7901064.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1835..2200)
                     /locus_tag="IVW65_002205"
     CDS             complement(1835..2200)
                     /locus_tag="IVW65_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7901065.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2392..2640)
                     /locus_tag="IVW65_002206"
     CDS             complement(2392..2640)
                     /locus_tag="IVW65_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901066.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2639..2767
                     /locus_tag="IVW65_002207"
     CDS             2639..2767
                     /locus_tag="IVW65_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7901067.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2824..4518
                     /locus_tag="IVW65_002208"
     CDS             2824..4518
                     /locus_tag="IVW65_002208"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7901068.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4600..4791
                     /locus_tag="IVW65_002209"
     CDS             4600..4791
                     /locus_tag="IVW65_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901069.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            5169..6302
                     /locus_tag="IVW65_002210"
     CDS             5169..6302
                     /locus_tag="IVW65_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7901070.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6333..6653
                     /locus_tag="IVW65_002211"
     CDS             6333..6653
                     /locus_tag="IVW65_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901071.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6682..7146)
                     /locus_tag="IVW65_002212"
     CDS             complement(6682..7146)
                     /locus_tag="IVW65_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7901072.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7275..8524)
                     /locus_tag="IVW65_002213"
                     /pseudo
     CDS             complement(7275..8524)
                     /locus_tag="IVW65_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8553..8870)
                     /locus_tag="IVW65_002214"
     CDS             complement(8553..8870)
                     /locus_tag="IVW65_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7901073.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8896..9216)
                     /locus_tag="IVW65_002215"
     CDS             complement(8896..9216)
                     /locus_tag="IVW65_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7901074.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3045 a         1860 c         1684 g         2847 t
ORIGIN      
        1 atgtgggcaa ttctatgtaa ataaagaagt caatctagaa aagctctcca atctcctgca
       61 tgtttgcaaa accactttgc tcaatgatat caataacata aaaaaagaat ttgaagaaca
      121 gatcgcttat acacatcggg aaaaggaccg ttacacttta tatttttcag aacatatacc
      181 tcgatgcaag atcatgcagc aattgtctca aaattcgttg tttttaaaaa cttgtctgct
      241 ttatttggaa gaagatgaac ctgattatct tcaattaact gaatgtgaat tcatctctgt
      301 cagcaaagct tattcattaa aaaaacaagt attagcttat tttaatgact gcgggatcga
      361 aatcgaccgc tactcccctc gattcactga aatggaaaga cgtctgctgc ttcttaatgt
      421 gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct gaatcgtttt ttgaacgtgc
      481 cgatcgtttc attgaatccg taacggaaaa tagtggcaga ttttatgata aagaaaataa
      541 agaaatctta ttgattggtt ttgctatcag tttcctacgc cagcaagtgt gtgctgtgac
      601 aatagattca aaatttatcg aagaaatcaa aaaacgtcct atttacaatt atgtcgaatc
      661 agcatgggaa aacactgatt tccaaaccta ttataaaaaa gaagaattcg cctttatttt
      721 gacactattc aatctttgta attatggttt ccattcttat cagttgattg cggaagactt
      781 tcaacagcta caccaagtgt ttattgacaa tactcccgaa ataaaagaac tagttgctac
      841 ctttgaatct cacttcaatc aagaattatt cggaaaccaa ccattcgaac gtgcgctgat
      901 tcatctgatg cgttcggctt gggataacta tcaattattc atgccagaaa aattttatct
      961 tctaaatgag gaacagacaa atttatataa agaagtccag acaatttttt ccagctggtc
     1021 cagccagtta ccttatgatc tacggttgaa tcctaattgc atgcgggctt ttgtgatcga
     1081 attgagcgga attctccgtc tgacaaaaga acatctgact atctatatcg tcacaaattc
     1141 ggatgttcat tatctgattt atcgggaagc cttggaagct gtaactactt tcgattttca
     1201 agttgcacca acaatttatt caagtatatc tgatataaag aagtatgcgc agcagtcctc
     1261 aaaccgggta ttatgtgaac gtacattata tacaccagat gcggtccaat atgaaaatat
     1321 tattcctatt tcgatcaata cgatcgatcg agctatcatt tcagctgtgc aaaacaaata
     1381 agggactgtg acaaaaatca tgtcacagcc tcttatttgt tgaagatgcc catttctgta
     1441 acaatctctt gatacacatc aggctcacaa cctcacttat tttggacatc tacgggtccg
     1501 atctctttat ttaaaaaata agaaatgatg gttcggataa ctaccaacgc cgctagttgg
     1561 aaaatatcct gcaaggtagg tttgataatc gattcgatga tcccagctga aacaagaatt
     1621 tccaaactta gaagtatata gcctcccaac atctttttaa tagcattgtt ctcgctgact
     1681 cggtcgcttt gcacttttgt atggacaaat aaatatttca aaaaatcctt gattgataga
     1741 caaacacccc aaaccaataa acagatagca aagatatcaa gtcccagtac aagtttttca
     1801 aaatatggaa gaagaaactg catcaactct gtcgtcatcc tacctctcct cttctttgtt
     1861 tttctttgtc ttctatttcc tgattcaaga aataagaaat gactgttcga atgataataa
     1921 taagtgcaag cttcaaaata tctgtccaag tcggcttgat aactgactcg atgatatcag
     1981 caacgactag tacttccaag cttaacaaaa tataacttgc tagcagtttt cttgtatctg
     2041 cattacgagt attacgttct gcatagtttt tcttgacatg agaccaatca aaaagtttga
     2101 atagaacaag aacgatcccg acaattaaga taccgataga acaaatattt aaaatagcaa
     2161 tgatccattc tagaaattca attaaatttg agcgaaacat atacgctacc tactttcttt
     2221 taatttgttc ctattctttt attgtaaaag aaagttacct tgttcgtcta tttaaaagtt
     2281 gttctaatga taacggttcg cttaaatttt tctttttcac tctccccttt ttctccctcc
     2341 atccataaat cgtggaagta tccaaaacaa cgtagcaagt taaaataagg cttagtccgt
     2401 catcagcttt tcaaagaagc actgtctcgg tgcttttttt gtacccaaat ttctaaaaat
     2461 agaaccatac acctcctcaa atcttttctt cttacctacc ctatttttat ttacaggaaa
     2521 atcagacact tcttcttttt tgtcaaaatt tcctttttct tctcgtccta tcatttggca
     2581 ttctaataaa aacgaactat ccttggatat agaaagagaa aacaaggagg cgtttttcat
     2641 gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg cttgcccttg gcgctgcata
     2701 tctaggaaaa aagacaggac tattcgaaga tgacagtcat ttatatgatg aattcgaatc
     2761 aatctagcat cctatttagt tgaatcacta cggacatact tggaagacat tggaggaatc
     2821 acaatgagaa aaagtaaagc aataatgatt ggtgccggta ttgccaatat ggcagcggct
     2881 gtttatttga ttcaagaagg caaatggaaa ggggatcaga tcacctttta ttcattggat
     2941 gatcacggtt caaatgatgg tgcaccgacc gacactgtag tggatgaata ttggaataaa
     3001 aaccatccat tagaaaacca aaaaggctat attgctcgag gcggtcgcat gcttaattac
     3061 cgtacctatg tagatttgat ggatctgtta agtcgaatac cttctgttac tgagccaggg
     3121 atgactgctg aagaagatac gagagatttt gatgcaaaac accgaacctt tgacaaagca
     3181 cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa aactgggatt caataataaa
     3241 gaccgtattc ttctgactaa actgattgct atgcctgatt cagaagaaga aaaactagac
     3301 aatatcacta tcgaagaata tttcaaagat gatccacact tttttgaaac aaacttctgg
     3361 tttatgtggg aaacaacatt tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc
     3421 tatatgcatc aaatgattta tgaattcact caaatcgaac atctagtggg cgtcaatcga
     3481 acacgttaca atcaatacga aagtattatg cttcctttga tcgagtactt gaaagaacaa
     3541 ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg aattcaaaga aacaccgatg
     3601 caagatgaaa tcactgtgac tggtttgaaa atgaccaata cactgactca agaagaagaa
     3661 cacgttgtgg tcgatgaaga tacagcagtt attttcacta atgggtccat tacagattca
     3721 gctactttag gagattacga aacaccagct gtagaaaata tggattatgg tgcagcttct
     3781 agtctctgga aaaaagccag tgaacgtttc tacaacttgg gaaatcctga caaattcttt
     3841 gctgatcgag atgcaagcga atgggtcagc tttaccctga cgacgaaaaa ccatttgttg
     3901 ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa atgccttgaa ttcatttatt
     3961 tcaaccacac cgatcacgcc gttaggccaa aaagatgtca atatgtctat cgtcgtgcat
     4021 catcaaccgc attttacgtc tcagaaacca aatgaaacag ttatttgggg ttatttcctt
     4081 tatccaagac gacgtggcga atttgtggat aaacagtata ttaaaatgaa tggaaaagaa
     4141 atgctagaag aactgattgg acaactttca aaagtagatc ctggaccagt gaatattcgt
     4201 gaaaaagaaa cagaaatttt cgacagcgta ataaataata tccctgtcta tatgccttat
     4261 gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac caaaagtcat tcctaaacat
     4321 tcgaccaatc tggcctttac aggagaattt gtcgagcagc cttatcagat gatctttact
     4381 gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc attttgcggg tatccctatg
     4441 tcacgtcttg tcaaaacacc gcgttacgac aaagatattc ctactctagc tcgtgccgca
     4501 aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg ccctgcatca agtaataaga
     4561 acaaccaggc taaaacagct tcttatgttt ttgatttggt tggacttatt accgcagatg
     4621 caagcttttg aacaccgttt accaagaggt tgtgaaaaag ctgccctgca tcaagtaata
     4681 agagcagcca agctaaaaca gcttcttatg tttttgactt ggttggactt attaccgcag
     4741 atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aagtcgttta gtttcgagta
     4801 tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga cttaataccg
     4861 agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt tttgaaaaca
     4921 accatttttt caacgtttac tctcttgcca atccaacatc atctaacaga tacaactatt
     4981 tcaatgatta ccattacact aaaaaatcgg aaactcacct ctccgaacac aaatacataa
     5041 gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc
     5101 tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat ctgaaaggag
     5161 catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc agaaacgtag
     5221 atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca acagccattt
     5281 gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa gggtatgttg
     5341 taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa acattgaaaa
     5401 aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat ttctgcctga
     5461 atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc gggcttttcg
     5521 gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta agagttcctt
     5581 atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa aaagttttat
     5641 ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga gtaaaaaaag
     5701 gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa aaattcgctg
     5761 ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt ctagataaag
     5821 cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa gctggaacag
     5881 aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta ggaatggacg
     5941 gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaaac ggcacaatct
     6001 ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa gtaactggtg
     6061 tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg
     6121 tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgaa atgattgcag
     6181 aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca gaagcagcac
     6241 aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt
     6301 gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga aatacccatg
     6361 agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc cgcttctatt
     6421 tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg attttctata
     6481 tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc tatttttatt
     6541 tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg aacattttta
     6601 tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca tgagatttga
     6661 aaaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac gagcggtcgt
     6721 cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt
     6781 atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt ttctgatttc
     6841 cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa acagggcaca
     6901 aagatttggt gtcaaatcag ggcggttatg gaagtcgttt tcaataacgc ccgctcctcc
     6961 gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt ccttattagc
     7021 gattccagta cgaatacttt cctcatacac aacagcagga actccccatt ttgatgaaaa
     7081 ccacttcgca ctttgtttta ctaattctgg gtgttctctt aatttgatcc agttatatgt
     7141 aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac aagtctttat
     7201 gaaaattagc aatgacaaca atagtgatgt aaaaaaaacg gaaaacaatc acttgttctc
     7261 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
     7321 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
     7381 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
     7441 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
     7501 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
     7561 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
     7621 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
     7681 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
     7741 ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
     7801 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
     7861 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
     7921 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
     7981 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
     8041 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
     8101 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
     8161 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
     8221 tcttttcggt agccgttagc taaatgataa gcaagaaagc aatcaagaca gaaagaatgg
     8281 caaatgtacc gttccaagca gcagccccga atcctttcca tgattcacca ccaaaaattg
     8341 agttcatcaa ttcttggaac ccgggaatcg gtaagttatt gatcattact gctaatgctc
     8401 ctaagatcat taacggcata ctcaccataa atgagtcccg aatcgcaact aaatgccgtt
     8461 gtgcaccaat ttttgatgct acaggtataa aatgtttctc cataaatgca acaaatttat
     8521 ccataaatct tccctctttt ctttactgta gattacgcgt tcaatcgacg atacagatca
     8581 atgatctctt ttgctaaatc tgtaaaagct atggatgtca tcagatgatc ttgactgtgg
     8641 actgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt cttgtgtcag catctctgtt
     8701 tgagagtgat gtgcttggac taaagattcc tctgcatctt tgattttttc atctgctaaa
     8761 gtgaaatcac cttttttcgc agcagcaatc gcttccattg catcgctttt tgcatttccg
     8821 ccaaacatga tcagacccat gatggcttct agattttttt gatcttccat ttattgtatc
     8881 ctccgataat ttttattatt tgtttattag tttttctgct tggtcgagta ctttttcacc
     8941 attcatcatg ccatagtcag ccatattgat tatatctaaa ggaatgcctt taggtgctag
     9001 cttttcagca aattgatttt gcataaagcg tacttgcggt cctaaaagta aaacgtctac
     9061 atttttagat tctagttgat tatccgcatc tgaagcagat acagcaaaga tgtctgcttc
     9121 cattcctttt gcttctgctg ccttttgcat ttttgtgact aggagacttg tactcattcc
     9181 agctgaacat acaagcatga ttgttttctt ttccatttta aacacgttcc tttctatact
     9241 tacacttgta tataagcaag tagcgtgcca actttgaaag cgctttaaaa agttgatatc
     9301 atcacattta aggtctatac caagttgcat acacactaaa aaagaaagtg cacactaaac
     9361 acacaaatta atttttatta ccttttttct tttaacgtgc acaaaaaaag ttgtgacaca
     9421 aaacttgtca caactt
//
LOCUS       DACCWK010000098         9341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCWK010000098 DACCWK010000000
VERSION     DACCWK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.101"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..1589
                     /locus_tag="IVW65_002216"
                     /pseudo
     CDS             1..1589
                     /locus_tag="IVW65_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(1653..3824)
                     /gene="copB"
                     /locus_tag="IVW65_002217"
     CDS             complement(1653..3824)
                     /gene="copB"
                     /locus_tag="IVW65_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7901075.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(3844..6030)
                     /locus_tag="IVW65_002218"
     CDS             complement(3844..6030)
                     /locus_tag="IVW65_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7901076.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDALFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(6030..6239)
                     /gene="copZ"
                     /locus_tag="IVW65_002219"
     CDS             complement(6030..6239)
                     /gene="copZ"
                     /locus_tag="IVW65_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7901077.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(6252..6692)
                     /locus_tag="IVW65_002220"
     CDS             complement(6252..6692)
                     /locus_tag="IVW65_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7901078.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(6766..7305)
                     /locus_tag="IVW65_002221"
     CDS             complement(6766..7305)
                     /locus_tag="IVW65_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP7901079.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITGFPLLPLDLLWHTTDYSIQA
                     KKWFIQQQQSFMETNHSWIIEGNYTSTLGKRIKEADKIIWLQVPRCRAIYRVILRSLK
                     RKINKKTRPDMPETFSERFDKEYLEFLSFIWHFEETHEPEIQQLIQENHAQNKLIILK
                     TRGEKQEFLTKLKIESKNN"
     gene            complement(7799..9265)
                     /locus_tag="IVW65_002222"
     CDS             complement(7799..9265)
                     /locus_tag="IVW65_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7901080.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2644 a         2034 c         1653 g         3010 t
ORIGIN      
        1 accaatctct cgaggaaacg agtgaaccga tccgagaata tcttaatgat gaagggcttt
       61 atgacacact ccatgagtat caattaaccg gaagtgcaag aacaggcttc agtgagaatg
      121 atgaacagct tggtggacct ttatatgata atggcgaagt tgtttttaca gcgaaccaaa
      181 aaggaaatgg ctattggaag atcgaaaata aaaatcatta caccggttct ggatggatag
      241 aagatcagga cgcacccatc caaccaatcc aagaattccc tgttgacatc acgagctacc
      301 aaaccgttat agaagacaaa gaaaacatta cattgaccct aaaaaaacaa gctttcttac
      361 cttatcctta tggaaatact accttgactg atttttcatt agctagtgga caaacgcttt
      421 actatttgcc tgaaagccag cgattcattc ctgtcccgaa tgaatcgaca cgttctgcaa
      481 ctttacaaac agaaaggtta gtttttacga gagaagaatt aaaaagcagt cagttaaaag
      541 ctgatgattt agaaaatcag cagacaaatc ttcaattgcc aaaaagtttg cctaaacgta
      601 ttgaggactt ggcggtcgaa ttgaccaaaa atgaagcaac tttaattcgt aaagtcgaag
      661 ctgtctaaca gtatttaaaa acaagcggaa atttccgcta ttccaaaacg gataccgggc
      721 atactccgaa agaacacgat tatgtggatt atttcctctt tgattcaaaa gtcggctatt
      781 gtgacaactt ttcaacttct atggtcgtcc ttcttcgaac acttggtatc ccagctcgct
      841 ggacaaaagg ttttacaaat gggctcagaa caggcacaga taatcaaggg aatgaggaat
      901 acagcattct caatagtcat gctcattctt ggccagaggt atactttcct ggatttggct
      961 ggcttccatt tgaaccaacc ccttcatttg ccaatccaat gggttcacaa ccagttgctg
     1021 aaacaaccga aaccgtccag cctgctatcg aacaaccaaa tgaaagcgaa aatatggcag
     1081 aaactatttc cagtgagaga tctgaggtca ttccatcaga agaaacaaca ctcacaccag
     1141 cttataaaat cgtcttattc agccttggag tgtgtttatc cgtcttgttg ttctttctat
     1201 tgttattcag aaaatctctg cgctggacaa gttatcttat ctatcttcat ttttttgctt
     1261 ctactaatac gattgcatat caaaaacttc ttaacccttt ggaaacattg gtaccgcgca
     1321 aacccgcaga atctttgcag gaatatagcc tgcgaataga acaagaattc aagctagatc
     1381 tgcgtttcat tcgattaact actcaatatg aagcagaaat atatggacga caagcagatc
     1441 cggtaaaatt cgataaaaaa acctatttag aaataacgca tataataaga aaaacggtat
     1501 acaaaaaaat tctgacaaaa gggagccgct tcaaaaaaca agataaaatt tctggagaat
     1561 ctacttctcg aatgccgaga accagctgat tgagtatcga ttactcgaaa aaaaggttgt
     1621 gacacgtttt ttgtcacaac cttttttcat tattatttga ttttcaatgt caaagcattt
     1681 agagctacaa ctaccgtact taacgacatc aagacagcac caactgctgg acttaatatg
     1741 ataccaattg gcgcaagaat tccggctgct agcggaattg caagaatatt gtaacctgcc
     1801 ccccaccaga ggttctggat cattttcttt cgcgtttgct tggctagatc aagaaaatgc
     1861 aaaatatctt ttggatcgct gtttgtcaat acgacatccg cagaatcgat tgcgatatct
     1921 gtccctgcac cgattgccat cccgattgaa gcacgggcta aacttggcgc atcattgatc
     1981 ccatcaccca ccatgattac ttttttccct tggtcaaggt atttctggac aacagcttct
     2041 ttatcatctg gtaataaacc gccataatat tcttcgatcc ctaaataatc tgctactgct
     2101 tgggcagcgt ttttattgtc accagtaagc atgacaggag tgatttttct ttctttcaat
     2161 gttcggataa attcttttgc ttctgttttt actacgtcac ctaaagcaac gattgcagct
     2221 agatgatttt ctaaaatcag gaagctgatg gtatttcctt gttcttggta aggtttcaat
     2281 aatgcttgtt ctacgtttaa tccgagacgc tcaacttctt tttcattaac tattttcact
     2341 tgttgttttt tgactgttgc ctctagcccg actcctgaaa gattttttag attttcagct
     2401 tgataaggct tgatttcatg ttctttcaaa taattcataa tgccaacagc caatggatga
     2461 ttggcattcg cttccaaggc tcctagatat tgaaggattt catttttatt gaacgcatca
     2521 tcaagtactt cgacacccgt tacagtaaat tttccttctg ttagtgttcc tgttttgtcc
     2581 agcatgatca catctagatc attggcttgc tccagtgcat tgcggttttt caaaagtaat
     2641 ccgtttttag ctgctattga tgtggagcga gcaacaacta atgggatagc taaacctaga
     2701 gcatgaggac aggcgatgat aaagactgtt accatacgtt ctaatgcatt aggaagatct
     2761 gtcaagaaca accaggcgat gaatgccagg atacccacaa ttaatgcgac ataaaacagc
     2821 cattttgcca ctttgtcaga taatgactct agcttggatt tttctccttg tgcttttcgc
     2881 accatttcca taaccttcgc tagataacca ttttcaccag ttccagttac ttcgatttcg
     2941 atcgttccat ccccattgat agaaccgcca atcactttgt cattggcttg ttttttgatt
     3001 cctcttgatt cgccagtcac agctgattcg tcaacaattg tttcaccttt caagattaca
     3061 ccatctgttg gcattttatc accagaccgg acgatcaaac gatcaccttc gtttacttct
     3121 ttcaatgaaa ctgtctcttc ttttccatct ttcgtcaagc gtttcactga ttctggcagt
     3181 aattctgcta atttttgtaa agcattgctg gcattcgaca cagcattcat ttcaatccag
     3241 tgacctagta gcataataac gatcaaagta gccaattccc agaagaaatc catgacgtgt
     3301 gtatgtggat tgagcagatt tgcgataaac gaatagacac tataaatata tgcaactgta
     3361 atccccatcg cgatcagtgt catcatagca ggactttttt gtttcaattc catcttagct
     3421 ccacttaaaa atggctgacc gccatagata aatagtatag ttgctaagat caatacgagc
     3481 caatcagaac caggaaacgt tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa
     3541 agaatgattg ggatagctag gataagcgat agccaaaatt tttgtttgaa attccccatg
     3601 tgcatggaat gatccatgcc gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt
     3661 ttgctgtttg ctatatctcc atggttatgt cccatgtgca tggaatgatc catctgacta
     3721 tggtcatgtt tcatctccat ctgtttttca tgagattctg tactttcttt gtttactact
     3781 tcttttttat gctcatgttt tttctccata tcgttcccat tcatttttac tccatccctt
     3841 ctcttacttt ttcatgtgtc gatttaagct cagggagttc aacaatacac tgacggaact
     3901 aaacgccatc gctcctcctg caatgattgg attcaaaaag cctagtgcgg caaaaggaat
     3961 accaatcgta ttatagacaa aagcccagaa aagattttgt ttgatttttc tcaatgtcaa
     4021 cctagacaac tcgattgttt gtgcaatgga tgccaaactg ctattcatca gcgtcacatc
     4081 ggctgtttcc atcgcaatgt ccgttccact tcccattgcc ataccgacat cagctaatgc
     4141 taaggccggt gcatcattga ttccatcgcc tgccataccg acttttttgc catctttctg
     4201 aagtttttct acgtaggctg ctttatcctc tggaagtact tcagcaaaga tatgctttgg
     4261 atcaatacca acttgtttac caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat
     4321 aaaaacatct aatcctttat tctggagctg cttgattgct tgtttggctt ctagtttgat
     4381 ttgatcagcc acggcaatca acccgatcac ttgcttttca tcagctaaaa acatgactgt
     4441 ttttccctgc tgttctaatt ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa
     4501 ttcaattaat cgtttacgtg tacccgcaaa atagcgtact ccattaatcg taccgctgat
     4561 cccagcacct gggtgggctg taaaatcaat catatcgtat gttgctgctt cttgcagttt
     4621 tccgtattcc acgattgctt tacctagtgg atgttcagaa gcttgttcta aagtataaaa
     4681 aatagatagg acttctttag aaccgactag atctgtaact ttgggctttc cttctgtaat
     4741 cgttccagtt ttatccaaaa caatgctgtc taagtgagca gctgcttcta atgcttctcc
     4801 gcctttaatt aaaattccat ttcgtgcacc aacacccgtt cctaccataa tagctgttgg
     4861 agtcgctaaa cctaatgcac atggacacgc aatgactaat acagagacac tatggatcaa
     4921 tgcgagttgc caatcaccag tgatcaaacc tgtagctatg agcgtgataa acgcgatgac
     4981 taatacgatc ggaacaaaaa tccctgagat ccgatctgcg attttttgga ttggtgcttt
     5041 gctcccttga gcatcttcca ccatttgaat gatttgcgct aatactgtct gcttgccgac
     5101 ttgtgtgact tccatgtgta gcagaccatt cgtattgatt gtcccgccaa aaagcgcgtc
     5161 atccggattc ttttctacag gaagattttc accagtaagc atactttcat ctatcgcact
     5221 gcttcctgaa ataattcgac catctgctgg aatctgctca ccaggacgga tgactagttg
     5281 atcaccaacg actacttctt caatcgctaa cgtttcttct ttcccgtttc ttatcacttg
     5341 ggcggttttt gtttgaagag acatcaattg tttgattgca ttacctgttt tcgtttttgc
     5401 tgtatgttcc aaatatttgc cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa
     5461 ataaagatct tgggggtgtc cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc
     5521 cgatgttcca attgctacca atacatccat atttggtgct ttcgtcttga ttgcatggta
     5581 cgcaccttta taaaaacgag caccgacata aaattggaca ggagcagaca gcactaactg
     5641 gacgattgga aaatgaaaaa agtgaacaat cgccgcatgg cttcctaaca tcatagcaat
     5701 cattgacaac atcaacggca aagtcaaaac cgtactcaat ataagatcga atagcatttt
     5761 tttcaaatac gcttgctttt cttctgcaat tttttgtttg tgggcttcat cgtacaaaat
     5821 tgcaccatag ccgatgtttt ctacgctttg gattaatttt tcagtggtcg tcccttcata
     5881 tttgacactt gctttttcag ttgccaagtt gactgtggca ttgaccacac caggttgtcc
     5941 gttcagctct ttttcgattc tagcagaaca atttgcacaa gtcatacctg taatgacgaa
     6001 cgtatccatt ttggtttctg ctgttgccat caaatcacct ctgctttata acccaatcca
     6061 ttgattgctt gacaaatctt ttcggattga acttttgttt catcaaactt gaccgtaccg
     6121 tttgcttttt tcaaatttac tttcactttt tgcacgccgt caagtgcgct tactgtttct
     6181 tctactcttg ctgcacaatg attgcagctc ataccttcga ttgaaaattg ttgtttcatt
     6241 ttttcttcct cctattgatg ttgtttacat tcacattgac ctgggataca gttacattcg
     6301 atcgtatcta ctggttgctt ttgttccagt tgtttcaaaa tctcattgat atcttcttga
     6361 gtcaaagtgg cttcttctac tagatccgca atcgtctctc caatcctttt tgcacagata
     6421 tgggaaaaaa gattttccgt cgcactgcga actgtttcca tctccgagac agctggatga
     6481 taaatgaatt tttttccttc ttgttctgtc cagatcactt ctttttttac caaacgtccc
     6541 agcaaagttt tgatcgttgc tggtttccaa tccatcgaat cagctaagat ctgcgtgatt
     6601 tcttgggctg tcgtctgtcc aagtgtccaa ataacacgca tgatctccca ctctgaatca
     6661 ctgatcttta ctggcgtttt gactttttcc atttatttca cacctttcgt ttacaaatgt
     6721 aatcaataac ttatggatac agattacact tgtaaacaaa aactgtcaat tatttttgct
     6781 ctctattttc aatttagtta aaaattcttg tttttcccct cttgttttaa ggataatcag
     6841 tttgttttgt gcatggtttt cttggatcag ctgctgtatt tcgggttcat gggtttcttc
     6901 aaaatgccag atgaaagaca gaaattccag atattcctta tcgaatcttt ctgaaaatgt
     6961 ctcaggcata tctggtcggg tcttcttgtt tatctttctt ttgagggaac gtaaaatcac
     7021 tcgataaatc gcccggcaac gcggaacttg tagccaaatg atcttatctg cttctttaat
     7081 cctctttcct agtgtagaag tatagtttcc ttcaatgatc catgaatgat tagtctccat
     7141 aaatgattgc tgttgttgta tgaaccactt ttttgcttgg atgctgtaat ctgtcgtatg
     7201 ccataaaagg tctagcggca gtagcggaaa gccggtgatt tctttgatct tccttgcgag
     7261 ggtcgatttt cccgacccac tttgtccaat cacgatgatc ttcattcttc tccctcactt
     7321 ctcttatcat tttgatttga tcataccact tataacgccg caaaaaaaca agggactgtg
     7381 acaaaagtcg tgtcaaagtc ccttgtttcg aataaatggt gttcaatcag ctgaccttca
     7441 gcattagctc aaaaagcggc aaaatatgag gagctatttt tccgtttgtt tctctaatac
     7501 tcaggtcaaa tcgctgattt tacaacctct tccttaaacg gtgttcaaaa gcttgcatct
     7561 gcggtaataa gcccagccaa acgaaaaata tgaggggcta tttttgtttg gctgttctta
     7621 ttacttgatg caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt
     7681 cttcgaaaaa caagccgaaa cttcacaaaa atttgagaag caattttcgt acattccttc
     7741 ttatttcttg aagctgagac cccttctgtc acaacctctt gtaacaagta tttctatttt
     7801 aaggctgaac cactgtttct ggttcagtga tttctttgtt tttgtctcga cgagcaattg
     7861 ctggcaagat catacctaat aagatcaaga cgattggtgt gatgatattc gtgaataatt
     7921 ggaaccacca tgctttagga tcagcagtat aatctacttt tggcaccatt cctaggatac
     7981 atgcaaacgc agtgaaggca aagcaccaag caccaaagat aaaaccgatt tttggatttt
     8041 taacaaattt atattcagaa ttgaatttct tataagcttt attcaacatc atatatgcca
     8101 agaacaccca taggtaacgc attggcatga caactgagtt caagttcgtc aaccatttta
     8161 ctaattcatt catatctttg ataccaaaga tcggcaacaa aataatgatt cctactagta
     8221 tacctgtcag aagataacca ttcaccaatg tacctttacg attacgttta cgtagccagt
     8281 ttggtacaaa ttctggatcc gcatctgcta acaagatttt tagtggtgca tcaattgaaa
     8341 aggctaatgc ggagatttgt ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt
     8401 taccgatacc atagtaatta cctaaaattt gaaaagcact gtatgcccca tttgccatca
     8461 agtctttagg aatattatcg ctagcaaaca gcatacccat tgcgactgag cctaatacag
     8521 cacaaatccc taccattgct gctaagaaga tcatcccttt aggaaattcc tttgcaggat
     8581 ttcttgtaga gttgacataa ggggatattt tttccgctcc tccaactgca aatactagca
     8641 tcgaaacggt tgtgaaataa ctgaaatcaa atgttggaat atatgttttc acttgattca
     8701 tatgtggtgt ggcaaattcc atcgttggat tgatgaacgg tgccgttaca gctaaaagaa
     8761 taaataagat cgacatgacg aacatcgctg ttccggccag tccaccaatc actttcaaag
     8821 tagaaagtcc ttttgttgat aaatataaaa aggcaagaaa tataataagt gaaataactg
     8881 ctaccatttg tactgacatc gtatttacga tatttccatt tccttggact gcccaaccgc
     8941 cagcaatcag gattgcttga ggcttttgtg ccaaataagg aatatggacg acccagtaag
     9001 tccaagcggc atagtatgcc agtcgtttat tagatgtcgt attttcgacc catgaactga
     9061 cgcctccttt actatccttg aaggctgatc ctaattgtcc aacgatcaat gcgtatggaa
     9121 taaaataaat ggccaaaatc aatagccaag aggtgacaac tgatactcct tgctgagcat
     9181 agttattaac tacattcccc atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa
     9241 accagcgtaa ttgtttgttg ttcattcgtt ctctccttta tgataaaaat ttgttatcca
     9301 gatgtaatca tattcaattt ttaacaaact tacaagacag t
//
LOCUS       DACCWK010000099         9293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCWK010000099 DACCWK010000000
VERSION     DACCWK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.102"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(24..692)
                     /locus_tag="IVW65_002223"
     CDS             complement(24..692)
                     /locus_tag="IVW65_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7901081.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            826..1113
                     /locus_tag="IVW65_002224"
     CDS             826..1113
                     /locus_tag="IVW65_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901082.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1529..3067
                     /locus_tag="IVW65_002225"
     CDS             1529..3067
                     /locus_tag="IVW65_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7901083.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3064..4029
                     /locus_tag="IVW65_002226"
     CDS             3064..4029
                     /locus_tag="IVW65_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7901084.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4016..5338
                     /locus_tag="IVW65_002227"
     CDS             4016..5338
                     /locus_tag="IVW65_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7901085.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5401..5505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5579..6322
                     /locus_tag="IVW65_002228"
     CDS             5579..6322
                     /locus_tag="IVW65_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7901086.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6337..7329
                     /locus_tag="IVW65_002229"
     CDS             6337..7329
                     /locus_tag="IVW65_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7901087.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7341..8078
                     /locus_tag="IVW65_002230"
     CDS             7341..8078
                     /locus_tag="IVW65_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7901088.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8068..8790
                     /locus_tag="IVW65_002231"
     CDS             8068..8790
                     /locus_tag="IVW65_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7901089.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8874..>9293)
                     /locus_tag="IVW65_002232"
     CDS             complement(8874..>9293)
                     /locus_tag="IVW65_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7901090.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3044 a         1603 c         1965 g         2681 t
ORIGIN      
        1 aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca
       61 aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac
      121 ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt
      181 gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt
      241 ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct
      301 gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc
      361 aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt
      421 aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa
      481 agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg
      541 aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta
      601 gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg
      661 cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt
      721 cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat
      781 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
      841 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
      901 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
      961 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
     1021 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
     1081 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
     1141 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
     1201 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
     1261 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
     1321 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
     1381 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
     1441 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
     1501 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
     1561 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
     1621 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
     1681 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
     1741 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     1801 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     1861 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     1921 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     1981 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     2041 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     2101 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     2161 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     2221 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     2281 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     2341 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     2401 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     2461 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     2521 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     2581 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     2641 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     2701 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     2761 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     2821 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     2881 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     2941 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     3001 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     3061 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     3121 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     3181 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     3241 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     3301 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     3361 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     3421 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     3481 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     3541 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     3601 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     3661 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     3721 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     3781 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     3841 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     3901 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     3961 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     4021 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     4081 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     4141 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     4201 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     4261 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     4321 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     4381 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     4441 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     4501 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     4561 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     4621 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     4681 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     4741 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     4801 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     4861 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     4921 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     4981 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     5041 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     5101 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     5161 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     5221 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     5281 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     5341 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     5401 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     5461 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     5521 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     5581 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     5641 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     5701 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     5761 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     5821 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
     5881 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
     5941 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
     6001 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
     6061 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
     6121 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
     6181 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
     6241 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
     6301 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
     6361 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
     6421 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
     6481 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
     6541 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
     6601 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
     6661 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
     6721 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
     6781 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
     6841 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
     6901 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
     6961 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
     7021 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
     7081 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
     7141 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
     7201 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
     7261 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
     7321 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
     7381 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
     7441 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
     7501 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
     7561 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
     7621 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
     7681 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
     7741 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
     7801 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
     7861 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
     7921 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
     7981 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
     8041 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
     8101 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
     8161 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
     8221 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
     8281 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
     8341 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
     8401 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
     8461 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
     8521 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
     8581 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
     8641 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
     8701 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
     8761 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
     8821 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
     8881 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
     8941 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
     9001 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
     9061 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
     9121 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
     9181 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
     9241 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DACCWK010000100         8942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCWK010000100 DACCWK010000000
VERSION     DACCWK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.103"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            51..260
                     /locus_tag="IVW65_002233"
     CDS             51..260
                     /locus_tag="IVW65_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901091.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IVW65_002234"
                     /pseudo
     CDS             467..667
                     /locus_tag="IVW65_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IVW65_002235"
     CDS             complement(1379..1984)
                     /locus_tag="IVW65_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901092.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IVW65_002236"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IVW65_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IVW65_002237"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IVW65_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IVW65_002238"
     CDS             3019..3990
                     /locus_tag="IVW65_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7901093.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IVW65_002239"
     CDS             4130..5443
                     /locus_tag="IVW65_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7901094.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IVW65_002240"
     CDS             5486..6352
                     /locus_tag="IVW65_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7901095.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IVW65_002241"
     CDS             6349..7197
                     /locus_tag="IVW65_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7901096.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IVW65_002242"
     CDS             7211..8683
                     /locus_tag="IVW65_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7901097.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="IVW65_002243"
     CDS             8700..>8942
                     /locus_tag="IVW65_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7901098.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DACCWK010000101         8683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCWK010000101 DACCWK010000000
VERSION     DACCWK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.104"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..302)
                     /locus_tag="IVW65_002244"
     CDS             complement(<1..302)
                     /locus_tag="IVW65_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7901099.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            488..1135
                     /locus_tag="IVW65_002245"
     CDS             488..1135
                     /locus_tag="IVW65_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HAP7901100.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1828..2766)
                     /locus_tag="IVW65_002246"
                     /pseudo
     CDS             complement(1828..2766)
                     /locus_tag="IVW65_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2766..3383)
                     /locus_tag="IVW65_002247"
     CDS             complement(2766..3383)
                     /locus_tag="IVW65_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901101.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(3915..5405)
                     /locus_tag="IVW65_002248"
     CDS             complement(3915..5405)
                     /locus_tag="IVW65_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7901102.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(5448..5747)
                     /locus_tag="IVW65_002249"
     CDS             complement(5448..5747)
                     /locus_tag="IVW65_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901103.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(6113..6397)
                     /locus_tag="IVW65_002250"
     CDS             complement(6113..6397)
                     /locus_tag="IVW65_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7901104.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6916..7401)
                     /gene="vanZ"
                     /locus_tag="IVW65_002251"
     CDS             complement(6916..7401)
                     /gene="vanZ"
                     /locus_tag="IVW65_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP7901105.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(7554..8465)
                     /locus_tag="IVW65_002252"
     CDS             complement(7554..8465)
                     /locus_tag="IVW65_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAP7901106.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2646 a         1549 c         1306 g         3182 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaaat ttaggaggaa
      481 gttatatatg acttttaata ttattgaatt agaaaattgg gatagaaaag aatattttga
      541 acactatttt aatcagcaaa ctacttatag cattactaaa gaaattgata ttactttgtt
      601 taaagatatg ataaaaaaga aaggatatga aatttatccc tctttaattt atgcaattat
      661 ggaagttgta aataaaaata aagtgtttag aacaggaatt aatagtgaga ataaattagg
      721 ttattgggat aagttaaatc ctttgtatac agtttttaat aagcaaactg aaaaatttac
      781 taacatttgg actgaatctg ataaaaactt catttctttt tataataatt ataaaaatga
      841 cttgcttgaa tataaagata aagaagaaat gtttcctaaa aaaccgatac ctgaaaacac
      901 cataccgatt tcaatgattc cttggattga ttttagttca tttaatttaa atattggtaa
      961 caatagcagc tttttattgc ctattattac gataggtaaa ttttatagtg agaataataa
     1021 aatttatata ccagttgctc tgcaacttca tcattctgta tgtgatggtt accatgcttc
     1081 actatttatg aatgaatttc aagatataat tcatagggta gatgattgga tttagttttt
     1141 agattttgaa agtgaattta attttataca cgtaagtgat cataaaattt atgaacgtat
     1201 aacaaccaca ttttttggtt gcttgtggtt ttgattttga atttggtttt gaacttatgg
     1261 actgatttat tcagtccatt ttttgtgctt gcacaaaaac tagcctcgca gagcacacgc
     1321 attaatgact tatgaaacgt agtaaataag tctagtgtgt tatactttac ttggaagatg
     1381 caccgaataa aaaatattga agaacaacta gcaaaagatt ttaaagagtt attttatttt
     1441 aagtctttat aacatgagtg aagcgaattt ttaaatttcg atagaaattt ttacatcaaa
     1501 aagccccctg tcaaaattga cgaagggggt tttttggcgc acgcttttcg ttagaaatat
     1561 acaagattga aaatcgtgta taagtgcgcc ctttgttttg aacttagcac gttacatcaa
     1621 ttttttaaaa tgatgtataa gtgcgccctt ttaaattttg agtgattata ttttttgagt
     1681 tagaaaaagg gattgggaaa atttcccaaa ataatttaaa aaataagcaa aaattttcga
     1741 tagagaatgt gctatttttt gtcaaaggtg tataccttga ctgtgcttgc tgttacatta
     1801 agtttatttt taagttatta aaaaagaaat agcttttaaa gtttggctcg ctgtcgcttt
     1861 ataaagctga ttgacttttg attgcaaact acttaaagaa aacaaactcg gactattcgt
     1921 tttcttctct ttggtttgaa catcagcaat tatcccctct tgattgccta ttttagcttg
     1981 tttagaagaa acaaaagcta aaagctcctc ttgggtttta aaacgctgtg tggggcttag
     2041 aacgccctta aacgaccctt ggtttacttt tatactagct tccacctcga aaaaaggttc
     2101 ttttttaaaa ttctctatgg cttcctggcg ctgaaaaata aggtataagg tgggcgtttg
     2161 aacacgtcct agtgaaaatg taccttgtac gcccttctgt tgtaaattta acgtatacaa
     2221 agggcttgcg ttcatgccga tcaaccaatc ggcaatttgg cgtgtttgcg cttcttgata
     2281 aaagggatag taattcattc caggttgcaa attttgaaaa ccgcttcgga ttacatcttt
     2341 ttctaagcta ttgatccata gtcttttaaa tgttttatct tttgaaaagg catttgcttt
     2401 atggataatc gaccaggcga tattttcacc ttctctgtcg ctatctgttg caacaataat
     2461 tgtatttgcc tttttgagaa gttctgcaac aattttaaac tgctttccct tatcttttgc
     2521 aacttcaaaa tcgtatcgat caggaaaaat cggcaaagat tcaagtttcc aattttgcca
     2581 cttttcgtca taatgacctg gttctgctaa ttccactaaa tgcccaaaac caaaggtgat
     2641 aaacgtttca tctgtaaata gtgggtcttt gatctcaaaa taaccgtctt ttttggtgct
     2701 ttgttttaaa gcacttgcgt aggctaatgc ctggcttggt ttttcagcta aaataaccgt
     2761 actcattaac tatccctctt ttcattgttt tttctttgat cgactgtcac gttatatctt
     2821 gctcgatacc ttctaaacgt tcggcgattg attccagttt gttcttcaac ttctttatcg
     2881 gataaaccat tcaaaaacaa atcgaaagca tgctttaaac gtggatcatt ttctttaaat
     2941 ttatgctgac gacctttgaa tttgcctttt ttcttagcaa tttcgattcc ttgtgcctga
     3001 cgttccttaa ttttttttcg ttctgattct gcttgatact tgtacaattc aatgacaagg
     3061 ctattaatca aacgccttaa attttcatct tcaataccat tcattgaggg taaatttaag
     3121 acttccaggg ttgccccctt aatttgaatt tgattcatca attctgttaa ttctttatta
     3181 tttcgtccta atcgatctaa ttcagtaaca ataacaatat ccccttcacg aatatagtta
     3241 agcatagctt gtaattgtgg gcgttcgacc gattgaccgc ttaatttgtc tgaaaagacc
     3301 ttagaaacgc cctgtaacgc ttgtaattgc cgatctaagt tctgttcttt gctactgaca
     3361 cgtgcataac caattttagc cattttcaac caacctctaa aattctctcg gttgcaataa
     3421 ccaatcagca atatctactt tttcaatttc aaattgctta tcagaaattg tcttttcgta
     3481 agcgataaaa tcttgcgcat attgttgctc attaaaaata gccaccactt cgtcattttc
     3541 taaaactcga taaataaatt ttttcatttt actcctccta ttatgcccaa cttaaatgac
     3601 ctattcacca agtcaattat actgctaaaa tcatattagg acaaataggt atactctaat
     3661 gacctataaa agatagaaaa ttaaaaaatc aagtgttcgc ttcgctctca ctgcccctcg
     3721 acgttttagt agcctttccc tcacttcgtt cagtccaagc caactaaaag ttttcgggct
     3781 actctctcct tctcccccta ataattaatt aaaatcttac tctgtatatt tctgctaatc
     3841 attcgctaaa cagcaaagaa aaaacaaaca cgtatcatag atataaatgt aatggcatag
     3901 tgcgggtttt attttcagcc tgtatcatag ctaaacaaat cgagttgtgt gtccgtttta
     3961 gggcgttctg ctagcttgtt taaagtctct tgaatgaatg tatgctctaa gtcaaaagaa
     4021 tttgtcagcg cctttatata gctttctttt tcttcttttt ttactttaat gatcgatagc
     4081 aacaatgatt taacactagc aagttgaatg ccaccatttc ttcctggttt aatcttaaag
     4141 aaaatttcct gattcgcctt cagtaccttc agcaatttat ctaatgtccg ttcaggaatg
     4201 cctagcactt ctctaatctc ttttttggtc gtcactaaat aaggcttgta tacatcgctt
     4261 ttttcgctaa tataagccat taaatcttct ttccattctg acaaatgaac acgttgacgt
     4321 tcgcttcttt ttttcttgaa tttaaaccac ccttgacgga caaataaatc tttactggtt
     4381 aaatcacttg atacccaagc tttgcaaaga atggtaatgt attccctatt agccccttga
     4441 tagttttctg aataggcact tctaacaatt ttgattactt ctttttcttc taagggttga
     4501 tctaatcgat tattaaactc aaacatatta tattcgcacg tttcgattga atagcctgaa
     4561 ctaaagtagg ctaaagagag ggtaaacatg acgttattac gccctattaa acccttttct
     4621 cctgaaaatt tcgtttcgtg caataagaga ttaaaccagg gttcatctac ttgttttttg
     4681 ccttctgtac cgcttaaaac cgttagactt gaacgagtaa agcccttatt atctgtttgt
     4741 ttgaaagacc aatcttgcca ttctttgaaa gaataacggt aattaggatc aaaaaattct
     4801 acattgtccg ttcttggtat gcgagcaata ccaaaatgat tacacgttag atcaactggc
     4861 aaagactttc caaaatattc tcggatattt tgcgaaatta ttttggctgc tttgacagat
     4921 ttaaattctg attttgaagt cacatagact ggcgtttcta aaacaaaata tgcttgataa
     4981 cctttatcag atttgataat catagtaggc ataaaaccta aatcaatagc ggttgttaaa
     5041 atatcgcttg ctgaaatagt ttcttttgcc gtgtgaatat caaaatcaat aaagaaggta
     5101 ttgatttgtc ttaaattgtt ttcagaatgt cctttcgtgt atgaacggtt ttcgtctgca
     5161 tacgttccat aacgataaac gttgggtgtc caatgtgtaa atgtatcttg attttcttga
     5221 atcgcttcct cggaagtcag aacaacacca cgaccgccaa tcatgcttga ttttgagcga
     5281 tacgcaaaaa tagccccttt gcttttacct ggcttggtag tgattgagcg aattttacta
     5341 tttttaaatt tgtactttaa caagccgtca tgaagcacag tttctacaac aaaagggata
     5401 ttcattcagc tgttctcctt tcctataaat cctataaaat aggttgttta attaacttgg
     5461 tttgcttttt cattcaactg tttcaatatt gcatgttttg aaaaagattt ttttccttta
     5521 taagtcaatt tttttccact aatcgaataa attattttgt tattttctat taacttatat
     5581 atataatctt ccccctccga agaaaaatac ttatctgatt ttgtttctaa gtagatattt
     5641 ctcttttcta actctttctt aaacgtttct agtgtataga tatttgctaa ttttcttatc
     5701 tccaataaac tattttttat ataagtttta cattcatcat gattcataca aactccacct
     5761 tctataaatg aatacaaaaa aagcaatcaa acgatttccg attgattgct taacaattct
     5821 taaattcagt agcttagata cttgaaaact ctctgatttc cctatataat gatagtacgg
     5881 ttatataccg tcttcaaaca aagttaatta aataacttct tacgagggaa gagttcatct
     5941 gactaactga taagcgttgg tttggcaatc ttatcgggct atgcatttat aaaatgtcgt
     6001 caaacatttt ataaatgtgt catggctctt ttttcgtttc tattcagttc gttgtttcgt
     6061 tatatctagt ataccgcttt taaaaaaaat aagcaacgat ttcgtgcatt attcacacga
     6121 agtcattgct tttttcttct tccatttcta aatccaatgt tacttgttct gattctgttt
     6181 ctggttctgg ttctgttggc tcatttggga ttaaatccac tactagcgtt gagttagtta
     6241 actttgcaat ttgttctagt gtttttatgg ttggatctga ttttcctgat tctattcgtg
     6301 aataatttga tctactcatt tctaattctt gggagaattt ttcttttgat ttggtacctc
     6361 tcatcttttt taagctttct ctaaatgcta gttccaagtt tctcccccct ctttttgtgt
     6421 aataaaattc acgctttatt taaattatag cttttaatat cttaataaac aagtgattaa
     6481 tcacttgtga tttgtgatta ttccaaaagt aggtaatttc accaaccttt ttcaaaagtt
     6541 tttatcgacg attattggac tggatcgtgt ttcctaaagc gattctaagc gcatgtttaa
     6601 ttttaaagtg ggtaattctc tttaaaacgt cctacaaaga ttcaaaaaaa gcatggctgc
     6661 tagtgggggt accgtcagga aaatgcggat ttacaacgtt aaggaaattt attaaagaga
     6721 ccaattcctc agcactaaaa ttgataattg ggctttcttt gttactatag tgttaggtaa
     6781 gctttttact ttatcaccgg ttttcccctc acttcacacc tacgggcgag tttccccaca
     6841 tcccgttttc catctaatcc aattcattca atatctttat gtgtatgaaa gatcagattg
     6901 cttgatttag acatcttaca cgtaatttat tcttaaatgg gtacggtaaa cgagcaataa
     6961 tacgagcaaa agtataccta caaaaataat aactctgtct aatttttttt gattcatatg
     7021 cttattgctt aaaccatata atttcagtcc aagaaagcct ccaacagtat ttgtaattac
     7081 atctgttatg tctgtcgctc caatagcgaa gataaattga attatttcaa aagtaagact
     7141 taaaaccagt acaaaagcaa acttaggtaa aaatccgatt tctttaaaat tgacattcaa
     7201 aagcaagcca aatggaataa agattataac attatctatc atctctctga aattcccagt
     7261 agcagtaaat ggagtcaagt taagacttct ttgatgataa ttaaatactg ataaaatatt
     7321 gtattgtaat ttgaataaca ctaaccagat tagtgtcact aaatataaag ctagcaatcc
     7381 tctagataat atttttccca atataatttc ctctaattct tttgtttaag ttgtatcatt
     7441 agtatagcaa tgtcctagtt aacgtgggtt ttaaattaaa aagctaacgt taccccaaaa
     7501 gtgctagtac aataatgctg ttcattaatt tcgttggttt cattccgcca tccttacctc
     7561 cttgaattag tatgtgttga tccgggaaac actgtcacaa ttacaccgtc tatattgttt
     7621 cctgatatct cataacgaag attagtcggc acatgaatgg tggtattttt agtaacagga
     7681 taataaaaga tctcatattt ttccccattt acactaacag aaatggtttt ttcttctttt
     7741 aggtaatcca tatattcctc gagaacgaaa ttcttttctt tcataatcgc actatgtggt
     7801 aaaccaacat agcgaatatg ccatggttca tattgaattc ctgttaactc tgttttgtcc
     7861 tctggataac gtaaaatgaa cccgtatttc caagcatttt cttctatcca ctttccttca
     7921 ggggctcgtt ccattttcgt caagcttgat cctacatcta gtgataaacc tgaattatgc
     7981 tcactataac ctgctggtaa ggcatactca gcccccattt cttggtaaag cacactttgc
     8041 tcatcaaagt ctcgatagcc actattaata ataaaatgac taacgccacc ctttacagca
     8101 tcattgacca tctctgaaaa tttttgtgct atttcttttg acatataaat attactatca
     8161 agcaacccgt atccatttat taattcgtca tgtttagata aattcacgat atctgacttc
     8221 acactttctt ggcgaacagg atatttacta ttgattaata gcagatttcc ttgataaacc
     8281 tgttcttctg taattgtttt ctcttgggta ttttcagaag tcccactatt ttctaaatgt
     8341 tctttgggat tttgatcata attttgaaat tcgacttttt cctgagaaaa cagtgcttca
     8401 ttaacgtagt cataacctaa gtatattaag aataacaata acaataaaaa aaacaacttc
     8461 ttcattttca gtctcctcct taactatata gttggaagta tgaaatattt ctagaatctt
     8521 ccccttaaac ggcacaatct cgtttgagat tgtgccgttt tgccccgtaa attacagtca
     8581 attatttcgc cctactggat ttactattat caccaatgta gatgaatttt cataagtgat
     8641 acccatgctg aatttaacga ggctgaactg cctgttgaca atg
//
LOCUS       DACCWK010000102         8482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCWK010000102 DACCWK010000000
VERSION     DACCWK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.105"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            163..1104
                     /locus_tag="IVW65_002253"
     CDS             163..1104
                     /locus_tag="IVW65_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7901107.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1173..1904
                     /locus_tag="IVW65_002254"
     CDS             1173..1904
                     /locus_tag="IVW65_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7901108.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="IVW65_002255"
     CDS             2048..4228
                     /locus_tag="IVW65_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7901109.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="IVW65_002256"
     CDS             4248..4730
                     /locus_tag="IVW65_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7901110.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="IVW65_002257"
     CDS             4746..5507
                     /locus_tag="IVW65_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7901111.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /locus_tag="IVW65_002258"
     CDS             5638..6972
                     /locus_tag="IVW65_002258"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7901112.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7279..7764
                     /locus_tag="IVW65_002259"
     CDS             7279..7764
                     /locus_tag="IVW65_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7901113.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7757..8440
                     /locus_tag="IVW65_002260"
     CDS             7757..8440
                     /locus_tag="IVW65_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7901114.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
BASE COUNT         2801 a         1485 c         1859 g         2337 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
     7081 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
     7141 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
     7201 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
     7261 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
     7321 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
     7381 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
     7441 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
     7501 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
     7561 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
     7621 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
     7681 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
     7741 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
     7801 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
     7861 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
     7921 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
     7981 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     8041 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     8101 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     8161 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     8221 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     8281 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     8341 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     8401 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     8461 ttggggctat accgaattat tt
//
LOCUS       DACCWK010000103         8457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCWK010000103 DACCWK010000000
VERSION     DACCWK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.106"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(61..2670)
                     /gene="clpB"
                     /locus_tag="IVW65_002261"
     CDS             complement(61..2670)
                     /gene="clpB"
                     /locus_tag="IVW65_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7901115.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2921..3967)
                     /locus_tag="IVW65_002262"
     CDS             complement(2921..3967)
                     /locus_tag="IVW65_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7901116.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(3978..4250)
                     /locus_tag="IVW65_002263"
     CDS             complement(3978..4250)
                     /locus_tag="IVW65_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7901117.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(4285..5193)
                     /locus_tag="IVW65_002264"
     CDS             complement(4285..5193)
                     /locus_tag="IVW65_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7901118.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(5325..6557)
                     /gene="pepT"
                     /locus_tag="IVW65_002265"
     CDS             complement(5325..6557)
                     /gene="pepT"
                     /locus_tag="IVW65_002265"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7901119.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(6572..7693)
                     /locus_tag="IVW65_002266"
     CDS             complement(6572..7693)
                     /locus_tag="IVW65_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7901120.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELIGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(7690..8391)
                     /locus_tag="IVW65_002267"
     CDS             complement(7690..8391)
                     /locus_tag="IVW65_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7901121.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         2326 a         1844 c         1457 g         2830 t
ORIGIN      
        1 aaggaggtct gacactagtt gtgtcagacc ccttttttct tttatagtct caatggaaga
       61 ttagacgatt tcttctagct cttctgtttt gaaatgaagt tgtccatcaa gtaaagtaat
      121 cgttactttt gatttaggca taacatgtcc tgcaacgatt tctttagcta gtggtgtttc
      181 tacttcctta gtgataaatc gtttcaatgg tcttgcacca tatgctggtt cataagcatt
      241 ttcagcaatc cacgttttgg cttcatcact gattgtcagc aggatttctt gatgttccag
      301 acgttgtgcc aattgcgcca ccattttgtc aacgattcct ttcacgttgt ctaggctcaa
      361 tggtgtaaat aaaatcgtgt cgtcgattcg gttcaagaat tctggtttga agtttccacg
      421 taatagcgtg ttgacttgtt cagccactgc ttctggaatc gttccatcag cagtcacgcc
      481 ttccagcagt aactgtgaac cgatattgct ggtcatgatc agaaccgtat ttttgaaatc
      541 tactactcgc ccttttgaat cagtcaagcg tccgtcatct aggacttgta acaagatatt
      601 gaagacatcg ggatgcgctt tttcgatttc atccaacaag acgattgtat aaggatttcg
      661 tctgacagct tccgttaatt gaccaccttc ttcgtaacct acatagcctg gaggagctcc
      721 gactagacga gacacagcat gtttttccat atattcactc atatcgatcc gtaccatatg
      781 atcttctgag tcaaacaagt tctcagctaa cgctttcgct aattcggttt ttccgacacc
      841 agttggtcct aggaaaagga aagaacctag aggacgattt gggtcttgca atccagcccg
      901 tgaacgaatt accgcgtcac tgaccgcatc aactgcttca tcttgaccaa tcactcgttt
      961 atgaagtgtt tcattcaatt taattagttt ttctcgttca ccttctacaa gtttcgttac
     1021 ggggataccc gttaatctgc caacgacttg agcaatctca ttttcggtaa cggactcttg
     1081 aaccatctta atctcgctgt cttttgcttt agcttctaat tccttcaatt ctttttctaa
     1141 ttgaggtatc gtaccatgac gcaatacagc agcgcgttct aaatcatagt tattttctgc
     1201 atcttctaat tcgtgttttg ctttatcgat ttcagcacgt tttgctgaaa cagaattgac
     1261 ttcttctttt tctgtttccc actgcatctt catcgcattt gcttcttcac gaagttcagc
     1321 taattcttct tgcaaatttt tcaagcgttt tttacttgca tcatctgatt cttttttcag
     1381 tgcagcttct tcgatttcca actgcatcaa acgtctagta acttgatcta attccgttgg
     1441 catagagttc atttctaccc gaatcgttgc actggcttca tcgatcaaat cgattgcttt
     1501 gtccggtaag aaccgatcag taatataacg gtcagataaa gtagctgctg ctactaatgc
     1561 gttgtcatgg atgttaacac catggtggat ctcaaaacgt tcttttaatc cacgcagaat
     1621 actgatcgta tcttccactg ttggttcttt gactaatacc ttttgaaagc gacgttccaa
     1681 cgctttgtct ttttccatat attgacggta ctcatctaaa gttgtggcac cgatcaaatg
     1741 taattcaccg cgagcaagca ttggtttcaa cagatttccg gcatccatac ttccttctgt
     1801 ttttcctgcc cctacgatat tgtggatttc atcgatgaac aagaggatac gtccatcact
     1861 ttttttgact tctttcaaga ctgcttttag tctttcttca aattcaccac ggaattttgc
     1921 tccagcgatc aatgcaccca tatctaatga aaaaatcgtt ttatctttta aattttcagg
     1981 cacatctttg cgtacgatac gttgagcaag cccttcaacg attgccgttt tccctacacc
     2041 gggttctcct atcagaacag gattattttt tgtttttcgt gaaagaatac ggatgacatc
     2101 acggatttct tcatcacgac cgatgattgg atccattttt ccgcttttga cttgctgtac
     2161 taaatcaacg ccgtattttt ctaatgcttt gtattgttct tcttggtttt gtgaggtcac
     2221 tctttctcct cctctcatct cttcgatgtt tttacgcaat tctttttcag ataatccgtt
     2281 tttattcaaa taaacagtta acggataatt ttttaatttc atcaacgcta aaataacgac
     2341 ttccgttgaa agaaactcgt cgccgaagct ttcacgtatt tggtctgctt cattcaataa
     2401 tcgaaagaaa ttctggctga ggttttgtcc atattggacg tttccaccag aaacaactgg
     2461 atactcatcc agtagacgat caatttcatg ttcaaaagat tcgacatcca atccggcatc
     2521 cgtatagaaa tttcgaccaa aatgattagg ttgtaaaaag attttccata catgggcaat
     2581 gtcgatatct tgatgttttc gagtgaccgc aatctgttga gcttcagcaa tcgcttcttg
     2641 aagcgtcgta gtcattttct caatattcat acaggtatac cctcctaatt aaaggtaaga
     2701 tcacttattt ctttgatctt tacaaaaact attatatcgc tttggtcaat tttggtcaaa
     2761 atatacacat ggttttttga ctttttttct cgacttatat aaatcttaaa aaaacaaaat
     2821 tgattgtatg tatataaaaa aagagccaaa caaacgtaaa attatcttat ccataacgtt
     2881 tttctttgat ttgataaaaa gggtagatct tataaaagac ctacccttga ttttctttat
     2941 tctctttttt tacttctatt atttcttctt tgatcactct tcgccgttca gcaatcacat
     3001 acacaaatcc atagagaaat ggcaagataa aggaactgaa acgatacagt aaaagcgaag
     3061 aaactgcgtc aactgttcca acaactggtc tgaataataa caaatagaca aattcaaagg
     3121 aaccaatacc agcaggcgta ggaataaccc ctgaaagcac tactgcaaag ctgataaacg
     3181 aaatggtcag tagaaaatct attttaggat ggttttcaat cagaatcaaa taaggaagta
     3241 cataccaaaa tacaagtttt aacatattcc atagataaat ccgcaatagc gcagtcctgt
     3301 cttggataat ggtttggacc gtttccctta aagagtagat ctgctgattg catgtatcca
     3361 cccatttccg caaacgctca cttttaaata acttattcga taagctgaca aaaaatattt
     3421 gaacattcaa gctgacggac atcgccaata aaaaggcaac aatcacgatc gtcaaaagga
     3481 taccagcgat gataaaccag atcattgatg gaccattaga ataaaaaaga gagaattgga
     3541 tgaccaagcc agctaatgca taagtgatca ctgccagttt gtacataatc atatggagtg
     3601 ctgtcacccc tgctccctgg gatagcttca ttcctttttt gttataaaaa taaatctccg
     3661 agatcagcgt tcctgtacca aacgaaacga ttcggtaaaa cgcaatatag ctagaggtca
     3721 agaacccatc tttcgttgta aaattcggcg cgaaaggttc agcaatttct ttgattgagc
     3781 gcccttcagc gatttggtaa actgtcccta atataacagc cgctgccaaa acaacaacac
     3841 tggtagacag caattcggca aagatatctg caaatgattg attgattaaa taataaataa
     3901 tggcaaaaat cacaatcagc aaaagcagat tcagaatgat tttagttttc gttcctttag
     3961 acgacatctc ctgctcctta atggaaaaaa taaatgagaa tcaaatcaac aagtacacct
     4021 gcagcgaatc ctatagtaaa cttctttttc cgagttttat gatggaaaac ttttcgagag
     4081 atcaatcctc ccaaaccacc gcctaacagg ctgacaaata tcaaattatt ctcaggaaca
     4141 cgccagccgc tagttctagc ctgatgttta tcatacccca tcagacaaaa taagtaaaaa
     4201 ttcacaatga aaaggtaaaa ccacaaaaga ttgtgtaaca atacatccat tactcttctt
     4261 ccaactttct gttaactact atttctaagc tttttcttct ttctttggtg aataaactgt
     4321 taatcctttt gatacaacag ataattcaac tggaagatca tctgtcgtgt ctccatccgt
     4381 tcgagtgccc acagattttt cctgcgcttg gattttcatg cgtttggctg tgaaataagt
     4441 aatgccgggg atcgtataga atttgcctct catgattcta ggcaaataaa aaataaatcg
     4501 gaaaatatct aaggaaggta cgatcgttac atgaaatttt ccatcatcta gggtcgctga
     4561 gtcgtcaaaa tttgtaaaac cgcccacgga gttagacatc gtgactgtca acagttggga
     4621 ttttcctttc caatgctcct cgtctgtttc taaatccaaa tgatattttt tcttcttcag
     4681 caataactta aaaaacttct tgataaagat ccacgagccg taacgctgtt tttcttcttg
     4741 actgatccaa actgctgtat ctgctaagat tccgattgtc agtgtagaga taatgacttc
     4801 atcattgact tgaccatagt ctattttccg tacattgcct tccaggatca cgtcagcagc
     4861 ttcttctttt ttgaggggaa tctcaagaac acgtgcaagg ttgttgacag ttccacctgg
     4921 aagtaatccg acatggtatt gagataatgt tttttggaac gcttggacga tatgatgaat
     4981 cgtgccgtct ccacctataa ttactaaagt atcagcttct atggcttctg ctttcttgac
     5041 taattccgca tcatcaatcg atgggccagt ttcagctagt tctacatata attctggctg
     5101 cttttctttt acataatctt gaaaccagcg tgctaatttc tcaccctcat ctttccctga
     5161 acttttgtta taaaaaacta gcagcttttc cattctttac ctcctattta ctcttcaaaa
     5221 aagagggcgg gacaaccaat tccattgtcc tgccctttag ccttagacag atgacattcc
     5281 atcattggga cgaacgcctt gtcataagct gttctttttc gattttaact ctttgtgttc
     5341 aattctgcaa tttttacaat cacgtctgtt gctttttcca ttacttgtaa agaaacaaat
     5401 tcgaatcgtc cgtgcatatt ttctcctccg gcaaatagat ttggcgtcgg aatacccatg
     5461 taagagattt ttgaaccatc tgttcctcca cgaactggtt cgatcactgg agagatacca
     5521 agctcgatca ttgcttgttt cgctaattcc acgatgctca tatctttttc aatgatttct
     5581 ttcatgttgt aatattgatc atacatatct actttgattc gttcttgatc aaatcgctga
     5641 ttcaactctt cttggatatg tgtgatttgt gctttgcgtg cttcgaattt ttcacgatca
     5701 tgatcgcgaa tgatataact catagaagct tcttcaggat tcccatccaa tgccatcaaa
     5761 tggaagaaac cttcgtatcc ttccgttttt tctggaactt catcggctgg aagctgatta
     5821 tgaaaatcga ttgctaactg tagagcattg atcattgtat cttttgccgt ccctggatgt
     5881 acatttttcc cttggatcgt aatatttgct tgagcagcac taaacgtttc atactgcaac
     5941 tcacctaaag gaccaccatc gactgtataa gcaaaatcta catcgaattg ctcaacatcg
     6001 aatttatccg ctccgacacc gatttcttca tcaggaccaa aagccacttt gatggtaccg
     6061 tgagggatag aaggattatt gatcaagatc tccattgcag tcatgatttc cgcaatccct
     6121 gatttgtcat ctgctcccag taatgtactg ccatctgttg tgatcaatgt gtggccttca
     6181 taatttttca aatttggaaa atcttttgta ttcaatgtat agcgttcttc cgcatctagt
     6241 acgatcgtag actcgccatc atagttttca atgatttgtg gattgactcc tactgcatta
     6301 aaatctgctg tatccatgtg ggaaataaat ccaatggaac gcacttcatg atcaacattg
     6361 cttggtaatg ttcccataac gaatccgtta tcttcattat agaaaacatc agctaagcct
     6421 aactcttcta attctttttt caattcttgc gcaaaagcaa cttgtgtctg agtcgatgga
     6481 gtcgttttgc ttgttgcatc ggagcgtgtt tctgttttga catatcgtaa aaaacgtggc
     6541 aaaagatttt cgtacatagt attctctcct tttaattaaa ttgaaatggg tttgtatttg
     6601 tttcagaaac gaaaaagtta atatcccaat tttcttcttg tttccaagat tcaaatttct
     6661 cgataaatcg ttgcttgcat aacgattcaa tataatgacc gggatcaatt gcaatcaacc
     6721 cagcgctttg catatcatga gccgtatgat aatagatatc tcccgtgata tagacatctg
     6781 ctctttgggc gattgcttgc ggataaaatt tttcaccgct tccaccgcaa atcgcaatgc
     6841 gcttcacaga ggattttgca ttttttggct ggactattct cagtccatct agttggaacg
     6901 cttcttttac ttgttctaca aatgcctcta tagaaatttc ttgcggcaat tcacctactc
     6961 gtcctaaccc aaacatttcg actggttcat ctatagcgaa aagatcataa gcaggttcct
     7021 cataaggatg agctgaccgc atggcttgaa tcacttgttt ttcgatagtt tccggcaaaa
     7081 taacctcaac ttttgcctct tgaacttgtt ctgtcttccc tactttccca atcgctggtt
     7141 gtgcaccagc ttccggagta aatcgaccgt gtccaatcga ggtaaaactt gtaccggtat
     7201 aatcaccttg tgtaccagca ccagctgcag ctaatacttc acgcattttc tgtgcatgat
     7261 ccacaggtac ataaacggcc agttttttat aatgaatctc atgagtcttt actaaataac
     7321 tctccacttc aataccgata agttcacaaa accagtcatt cagtccatcc caaataatat
     7381 ccatgttcgt gtgtgctgca tagacggcaa tatcatgttt caacaaatct gcatacattt
     7441 tttcttgtgg ctgatcagta accagccgtt ttacaggacg aaagattgga ggatgtttag
     7501 caatcagcaa atcgattttt ttcttgattg cttcttcgac cacttctgga cgaacgtcta
     7561 atgtcatcat tactcgttgg attggcttat ccaatgttcc gatatgcagt ccgactgggt
     7621 ctccttcttc agctagccac tgaggacaat agctttcaaa cttttggatg aaagtgcgtc
     7681 cgctgattgt catggcttta aaacctcctc aatcatttgg atttcctgct cgatttgttt
     7741 ttgacgtgcc tcttgattag ctgcttgttt gagctgttca cggatagctt ttctttgttg
     7801 aagctctctt gtccattttg atttgaagat ctcacttttc tcttcaagca gaaatggccc
     7861 gaacatcagc tccatttcat tgtaagtaac tggtttttgt tctttttcgg ctacaatgat
     7921 ttcatatatt tttttatttt cttcaagtat tttttctgat tggattttat actgattttc
     7981 ttgcagccaa gatcgtaatg tacgctctcc gatgtttggc tgaaggatca aacgttcttg
     8041 tccgcttaag cgttgattca acttgccagc ttctaggata tctcggatca gtgaacctcc
     8101 catacccgca atcgtgacag ctgtaatacc atcttgtggc tggatagcat ctagaccatt
     8161 tgcaaggcgt acggtgatac ggtcttctaa tccatctttt ttgacttgct tacgtgctga
     8221 ttcataagga ccttgaacga cttcaccagc aactgcatat tcgatttttc ctcttaatat
     8281 caatgcaacg ggtaagtaag cgtgatctga tccaatgtct gctaaacgag cggattcagg
     8341 gacaaagtca cccactgcgg ctaagcgctg agataaatct aaatggttca tagcttcact
     8401 ccttctagat aattatagca ttttccaaat aaaataagga gactaggaat aaaaaaa
//
LOCUS       DACCWK010000104         8373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCWK010000104 DACCWK010000000
VERSION     DACCWK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.107"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            359..1684
                     /locus_tag="IVW65_002268"
     CDS             359..1684
                     /locus_tag="IVW65_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7901122.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1677..2027
                     /locus_tag="IVW65_002269"
     CDS             1677..2027
                     /locus_tag="IVW65_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901123.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            2009..2194
                     /locus_tag="IVW65_002270"
     CDS             2009..2194
                     /locus_tag="IVW65_002270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901124.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(3098..3373)
                     /locus_tag="IVW65_002271"
     CDS             complement(3098..3373)
                     /locus_tag="IVW65_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7901125.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3345..4298)
                     /locus_tag="IVW65_002272"
     CDS             complement(3345..4298)
                     /locus_tag="IVW65_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7901126.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4910..6403
                     /locus_tag="IVW65_002273"
     CDS             4910..6403
                     /locus_tag="IVW65_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7901127.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6538..6834
                     /locus_tag="IVW65_002274"
     CDS             6538..6834
                     /locus_tag="IVW65_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7901128.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6935..7786
                     /locus_tag="IVW65_002275"
     CDS             6935..7786
                     /locus_tag="IVW65_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901129.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7807..8019
                     /locus_tag="IVW65_002276"
     CDS             7807..8019
                     /locus_tag="IVW65_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901130.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2912 a         1298 c         1561 g         2602 t
ORIGIN      
        1 gattaataaa ttattacaaa aactattaag aaaacttcta catacaacta ttacaaaact
       61 attaagaaac tgaatactcc tattttttgt ttttaaggaa agttttttct tttttgaatt
      121 tcttattttt ttctaatata actaagcaaa actggataat acgacactta ttttcaaatt
      181 ttcacaagac taatagtaaa tatccattta ataaagtgat acaatgtgtt gattttacaa
      241 atcaacagaa gaagtaggga aataacttac ctatgcttct ttttttgtac atttaatcga
      301 ttcttcttta caaaaacgaa cgtatgtttg tataatgtgg aagaatggag tgttatttat
      361 gatggattat tcaaaagagc cagtaaatga ttactttttg atcgatatga aaagctttta
      421 cgcaagtgtc gaatgtatag aaaggaattt agatccatta acgactgaat tagtcgtcat
      481 gagtcgagcg gataatacag gatcagggtt gattttagct tcttcaccag ctgcaaagaa
      541 gaagtacggt atttctaatg tcagtcgacc acgagatcta ccgcaacctt ttcctaaaac
      601 gttacatgtc gttccaccac gcatgaatct ctatattaag cgaaatatgc aggtaaacaa
      661 tattttcaga aggtatgtag cagacgaaga tttattgatt tactcgatcg atgaatcgat
      721 cttaaaagtg accagatcaa tgaatctttt tacgactgaa ggaacaagaa gtcaacggag
      781 aaagaagctg gctcaaatga tccaggagaa tattaaagaa gagctaggat tgcttgctac
      841 agtaggtgtc ggagataatc ccttattagc taaacttgct ttagacaatg aagcaaaaca
      901 taatgatgga ttccttgctg aatggacgta tcaaaatgtt cctgataaag tatggaatat
      961 tcctgaaatg acagaccttt ggggtattgg atctcgaatg aaaaaaagac tcaaccaaat
     1021 tggtatcatg agtattaaag acttagctaa ttggaatcca tacactatta agagccattt
     1081 aggtgtgatt ggcttacagc tgtattttca tgcgaacggg atcgatcgaa cagatatagc
     1141 gatcccacca gaaccgacaa aggaaaaatc atatggtaat tcacaagtgt taccacgaga
     1201 ttataccagg agaaatgaaa tcgaactggt ggtaaaagaa atggcggaac aagtagctat
     1261 tcgtatcagg caacataact gtaaaacagg ttgtgtccat ttatacattg gcacgtcgat
     1321 cttagaaacg agaccaggtt tttcacacca gatgaagatc cctataacag ataatacaaa
     1381 ggaattgcag aattactgtc ttttcttgtt tgataagtat tatgaaggac aagaagtaag
     1441 gcacgttgga ataacctata gcaagcttgt gtacactgat tcgcttcagc tggatttatt
     1501 tagtgatcca caaaagcaaa ttgacgaaga aaatttagat aaaattattg acaagattcg
     1561 gcaaaaatat ggttttacgt cgattgtaca tgcttcttct atgttagaag gcgctcgtag
     1621 tattactaga agtacattgg ttggtggcca tgcaggcggc gcaggagggc ttaaaaatga
     1681 ttgattataa tgatcgtgga cgtttaaagt gggcaggttt ttatttatct gatcatactg
     1741 aaaaaattga tgccgacaat gaacaacgag ccaatcaaaa tttggcaaaa gaacaaatga
     1801 cgactgaaga ggttgcggaa gtattgaacg ctgcagtcat aaaaaataga tcagtatgta
     1861 ttcaaaaaga agaacgaaac gctgaaggat actatccaga agatattgtt agcaagatct
     1921 taggtagtga tgagctgggg atctacgttg gaaaaaacaa agttcactac gacgaaatac
     1981 gtcatatcga attttaccag gataataaat ggggtcgatt agaatgaaca ttatccagca
     2041 atatgagcta aagtatataa cctttgatca gctatcagaa gagatttggg gttatggcca
     2101 gcgtttgata aatgaggttg gtgttgaacg cttctctttc tatgtagaag ctgcagcagg
     2161 ataccataaa tacgaatact atattggcta ttaaagtgca ataaaattat atttctttta
     2221 tgttttacat tttgtatcaa ttctgataaa attacctata ggtaattaaa aaatataaaa
     2281 aaactagtta caaccttagg acgttgtaac tagcttgaag catactagag ctaactaaaa
     2341 tagcactaga aagcaatgtt aaacccgtcc aacaaacttg atttaacatt gagaccagct
     2401 tattcagcta aatatagttg aataagcttc ttttgtttta aaaaaacaaa aggtgtacta
     2461 cgaataacgc aagtaaaaga aatactatta caaccagtag gctacaagta agtattacta
     2521 aacattgcgt agttagaacc tttgctctat tgtctaaagc cacaaaatta gagatcagct
     2581 ttatgatgct aagagcgagc tgcatccatg cggacatagg ttcttttgac actattaatt
     2641 ctccttttta ggataaggga aattgctagt agttttttca ttagcaccat cccccttcat
     2701 ttgattgagt ctagtgctgc tgtgatagtt tgctgtttaa tatgcttaca ttcttattat
     2761 aacataaatg aaacaaacac atggttagag gtcgtatcaa tcaaaatagt gtctcacaaa
     2821 cgatttatta cttactcctg ctgactaacg gactgttctt cagtaactgt aactatatgt
     2881 ttgttaaaac caccattacc tgtagataaa aaccaatata tttactatac tcactatatt
     2941 aaatatattt agtatagtaa aaactagaaa caataaaatt tgtgaatgtt gtataccccc
     3001 aaacaataca catattttga aggattagga ttaataaagc taaaaatata gtaagtatac
     3061 taaatatatt tactatatct agtatactta ctatatttta atcttttgat cgtctattat
     3121 atataatccg ttcctcgtcg tttaaagtat caacataggc gtccattgca agtttgacta
     3181 tttcatacat aggaatatct tttgcataac taagatcccg aatggttttt agtacgggag
     3241 gatctacttg aactggtttt ctttctttcg cagtgtactt tcgttcccct aactcgacat
     3301 taatatcatc tttaattgaa atatcttcag caccaatttt cttcttatct ttgttcgttg
     3361 gtaataatgc catttagttt aatctccttt ccaagaggta ggatatgatt ggccattgag
     3421 tcagcatatt taggtgtata atcaaatcct tcaatatcac catgttgttc atagtattca
     3481 attctttctt ctaattctgt aaagatgtct gcaaaaatag cccaccatct tcgatctaca
     3541 taatcattta attggatacc attctcacca tacatttcta accgatccga gcctttaaca
     3601 atgcttttaa aaatgtttga ttcaccatac ttatcaattg ttttttgata gtttgcgtgt
     3661 tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc caatgatttg aagatgagac
     3721 tcttcaaaat tcgtgacaat cggaactaaa actttattaa taaaatcttc tgttccttcc
     3781 attgcgtacc gttttaattc ttgcattgca ataatataat ctgcagcagt taagaatgca
     3841 cgaaccacat tatctgttga tggtgggcag tcgataaaga tataatcgaa attttttctc
     3901 aattcttcta aaggtttaat gaaggccaat gatctcttac gacgatcagg atatttttcg
     3961 gacaagtatt cattaagttc ttcagatcca ggagagccag ccataatata taaatgtgga
     4021 cttacttgag taatacctga aacccagtcc ccttttttga ttgctgcaga catggatcga
     4081 tcaaagctag taatttgata cgttgatcca atgttggaag tagtatttct ttgttggtca
     4141 taatcaatta gcaacacgtc tttttcaaaa agttgactag aaacaatcgc taataaatca
     4201 ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa gcattacaac ttttcctagt
     4261 ttatctgatg ttttaaatgc agtccgtaag ttttccatag ttccctccaa tttcctatac
     4321 taaatatatt tagtatactt catatactta atatattcga tatatctaat atactccaaa
     4381 ttgataagaa ttcagacttt ataaggaata tagtggatat actaaatata tttgctatat
     4441 tcagtatatc agatatattc aaaatgtcaa aatatatttg tgcgttttgc gttgacaatt
     4501 tagagtgaaa agaggtaaac ttaattctat agaaaccaca gataattcac tatttacaaa
     4561 gtagcaaaag cagtggtact ctttaaacag aaaaattaca taaaaaaaag agacggaaat
     4621 aattgatttt agcagaatca attatttctg aatcacatgg ttaggacatg tgttcacgct
     4681 cgtctcaaga atatgtaagt agctaccaac tacttatatt aaaagttgat cggatacctt
     4741 ttccgattgc ctttattcta ccaaaaaaag gcgagcaact caacagaaga acgtttattt
     4801 agtgtactta aattaaatat tcttaaacac tgaagagaga acacatacta tggctgagat
     4861 taaagctaaa ctgtgttctc tttttgtttg agaaaggacg ttacaatcga tgttgagcga
     4921 aatttattca gctattctta aaaacggctt acgtacatac aaatatcgaa acagcaccat
     4981 gaaacctgca ggtcatattg attccagtag gagtggtgct atctttgcgt accgtagcaa
     5041 gcaattaatg aatgttgggc gtggtatggt cattacttcg gaagaagcta ttttagagaa
     5101 cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc tttggtactt atgcggatga
     5161 ttatcgaaca gtggttaaag gtcattctga gaaaaactta agtcaaatca acacatttgt
     5221 agttgatatc gattcaaaag agaaccatca aggcgaaatt attttagcct gtttagatca
     5281 agtgggctac atgccaacgc taattttaga atcagatcat ggttatcaag tttattttgt
     5341 actaaagaca ccaggctatg tcacaaagaa aagcaatttc aaggtgatcg atgtagcaaa
     5401 aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa ttgtccggag tggatctagg
     5461 atgtaaccac ttcggaattg ctcgattccc acaagaaaat aatattgttt ttaaagaaat
     5521 gaactatcag tattcttttg ctgactggat tcaatggtcg atgaagcttg aatcagatca
     5581 aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt cctgagaaaa aagaaattcg
     5641 tcaagtagat gaaccgtggt tcgatatgtt acttcataaa gcaaacatcg tcggcggaga
     5701 aggacgtctt ggtcgaaata atgtgatttt cacgctttct ttagcttatt actcttcagg
     5761 ttatgcattg gatacttgcg aatacaatat gttcacattc aacgaacggt tacaggaacc
     5821 gctatctgaa gcagaattag taaagattgt acgtagtgct tattctggta attatcaagc
     5881 agcaagcaga gaattcatta ttgaattgtg ccagcaatgg gtttcaccaa ccttaaaaaa
     5941 taatgatctg ttcattcaac gacgtggctg gtggaaattt aaaaagccac gagatcaaag
     6001 aaaatatagt cataaaaatg aatggaaaga agatctattg gcttatctaa gcgaaaaaag
     6061 tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa ttggcttcac aactaacgat
     6121 gcccaagcga acacttgata agatcttaaa tgagttacga gaggagaata aagtcttcta
     6181 caaggtaaca cgaggaagaa atggcggaat gatcattgct tcagtcaaaa cgttgttcgc
     6241 gagggttatt caactaagta aagaaaagag actagattat tcaacaagga tcacagaggt
     6301 atttggactc aacgagagct tagtagaaga gacatttaga caattatcaa cccccttgaa
     6361 ccctcttaaa gaagtgaatc tttttgaagt agatacaggt tgaaaaataa aaggtgcaaa
     6421 ttgccctata caatatatct atgatacttc tgtaggtgtt gctccctttg cctagctacg
     6481 tgtaggtggt atacttataa ctgcgtatgc agtcttatgt atcatggagg aagttgtatg
     6541 tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt ttatcattgg tcgtttggga
     6601 ttatctgtgg aagtgttctg tgagctttat ggttttaagc aggggacttt gtcttcttgg
     6661 gttaccaggg aaaaaacagt tgcttctttg ccgattgagt ttcttcatgg gttgtcctta
     6721 gcttctgggg agactatgga tcaggtatat gattgtttgt gtgtgttaga gcaggagtat
     6781 attgagtata aaatagctaa cgagttaaga aaaaggaaga agtatatcca gtagtagttt
     6841 ttatttactt aaaaaattgg ttcattgagc aacctatcgc tgctaaagta gcaaaggaaa
     6901 acgttaaagg aaagaattta gggaagaaat cgaaatgaaa tcggacgact ggattgaaca
     6961 cacctggctt cctctgaaaa gaaatagcaa acgaaagaaa ttttgggtat tgcaattatg
     7021 gccattagga aaactttgta gttacggggc acgacttatt cttgatttaa ccgtttactc
     7081 gattggagta tttggaacgg cttattactt gataaatgat cccaatagga ttgcagcatc
     7141 agacattttt acagtgctaa tgacgttgat ttttgtcatg ctattgtcta tttttactca
     7201 attagtcatg caatttacca ggcaaaaatt aacagcagga aaacttttca aaagcctatt
     7261 cgatttaatt gttgtaaatg caattctagc gactttaata aaacaaattg aacaatctgg
     7321 aagaacaaca aaaggagtga aggatttttt attgacgttt attcactttg taaatggaca
     7381 catgtatttg tttgaagtgg gtattattct cttttttgtc gggtatgtat ccacgaaact
     7441 tattaaacgt tcaattgaaa aagaaattga aattgacctg attccgacac ttcttggtta
     7501 tgagtacaat gttgattttg gaagatatga cgaattgatc aatacggata tcgttcacac
     7561 gagtatccat gaatctactg ttaagggcat cagatctatt ttaaaaaatg gccaggaaat
     7621 gaaagcaata gatcgaagaa aagagatcta caatttttat gaagtcgaag caagcctacg
     7681 atatggcatc ggaaaattgt ggttagatgg aagtaatatt cgtcaaaaca aaccatacat
     7741 tttttggaac agtagtgatt tagaacttcc aatgaaagat aaataattat ggagaatcgt
     7801 ttgaaaatga ttcctctata caggattgtg cttatagttg gcatggagtt aaaaacctta
     7861 aagaaggagc agttattcaa agtgaaagaa cgtcataatc agttactatg tagtaaaacg
     7921 ggtacgatga acattgaata ccgaacagaa tatttagaat taacgaatca aactgatttt
     7981 ttgttgattc gaaagcaaca agagcaagaa aaaaattaat ggctacttat agccaattga
     8041 ttgatgaatg ggaacaattc aaggaatcta aaattccaga acgtctctaa aagcctttat
     8101 ttgtaaagag cataaggaaa cgtgggaaca ctttttaaaa aaagtgttcc aataggctgt
     8161 gcctattacg tttctcaaaa ataaatgtgg gaacaaaata tttaaaaata ggatagtgaa
     8221 aggatagtga aggatagtaa aaggagggta gtaggatagc aaaaaggata ggctcctttt
     8281 cgctatcctt tttaaattgg aggaattaat atggaagtaa gaataagaaa tgtggatgag
     8341 aaaatcgtaa gagaaattga tcgaagatgt gac
//
LOCUS       DACCWK010000105         8367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCWK010000105 DACCWK010000000
VERSION     DACCWK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.108"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            7..2199
                     /locus_tag="IVW65_002277"
     CDS             7..2199
                     /locus_tag="IVW65_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7901131.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="IVW65_002278"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="IVW65_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7901132.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLTVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2670..3071
                     /locus_tag="IVW65_002279"
     CDS             2670..3071
                     /locus_tag="IVW65_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7901133.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            3086..3985
                     /locus_tag="IVW65_002280"
     CDS             3086..3985
                     /locus_tag="IVW65_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7901134.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="IVW65_002281"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="IVW65_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7901135.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            5324..6241
                     /gene="glyQ"
                     /locus_tag="IVW65_002282"
     CDS             5324..6241
                     /gene="glyQ"
                     /locus_tag="IVW65_002282"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7901136.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            6243..8318
                     /locus_tag="IVW65_002283"
     CDS             6243..8318
                     /locus_tag="IVW65_002283"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7901137.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVAPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2831 a         1524 c         1795 g         2217 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaagaaa aacttggaaa ggcagcattg
       61 cctttcctgc tgttcttgtt ttttatcatt atctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa
      181 acgatcgaga acacggaaga aaccgaacaa aaaagaaaac tggcagcaga agcagtcact
      241 ccagaataca cttaccaaaa agatcttgca gatgaccaga acaatcggat caagcaacta
      301 tttgaattga tcgataagac gaatgatgcc atcaacaaat cttatgacga aaagattgat
      361 aaggcaaagg aagatgaaaa tatcccaaag cctactacag aagagcggat cgccgcgctg
      421 aagaaaaatt ttgaaaatgt gaatgcagaa aatgttgtgt tttatcaaaa actaccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaactgaga ttcatactgt gagggatgag
      541 agtctgaagc tcattgacga acagatgagc aaacaagttc gtgaaagtga attggaagct
      601 tttaaacaag aagcagaaga gcaaatccaa tatttgaatg tcacaccagc acaacaacaa
      661 atgattcgct acttagtgga tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag
      721 acagaagaat tgaaacaatc cgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaatcattg ttcgtgaagg taatcaaatc gatgcttcag ctatgaaaaa attggaatta
      841 ctaggactga ctaatcaaac aacttcgatt tttccgctaa ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggcgtact gatttataat tcacttcaat tccaaaatgc aggagaaaga
      961 acaaaatacg tgcttttcta tgtgacagct atgtcagtca gcatagtgct gatgaaattt
     1021 ttccaattat tccaaacgga acaagctact tatatcccgc tgttttatcc cgctgctttt
     1081 gcaccacttg ttttaagctt tttcttgaat cgaagagcag ggatcatggc tggtattttc
     1141 caagcagtat ccgcactttt cattttttat gattcgattg gcacgaactt tctaacgatt
     1201 atcttgatgt cttatgtttt ctctgggctc atggcaacga ttgtcagacg caaacggatc
     1261 tcagaacaag gattatctgc tgccatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatcg tctatcaagg aatgagcttt ggtgatgcaa cgacttggtt aatgctcgtc
     1381 tgcgggttca ctggtgcact cttctcattc ttgctgacga tcggtctaca cccatatatt
     1441 gaattgatgg tcaatgatga cagtgtaatt gtattaaatg aactcagcaa tccaaatcat
     1501 cctttattga aacagttgct ggaagaagcg ccaggtactt atcatcatag tatgatggta
     1561 gctaatctaa gtgctaacgc tgtagcagaa atcggcggac gttctttgct aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacatgcta gctttttcgt tgaaaacttg
     1681 ccttcaggag cggaaaaccc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg agccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcaaacagca tcatggaacc acgctaatgc gttatttcta catcaaagcg
     1861 aaagaacgca atccggaagt aacagaagag caattccgtt atcctggacc aaaaccgcaa
     1921 agtcgtgaag cagggatcgt caatattgct gacagttgtg aggctgcagt acgggcaatg
     1981 gatcatccga cgagcgaaaa aatcgaaagt tttgtccata atcttatcga agagcgtatt
     2041 tccgatggtc aattagacga tagtggactg accttgaaag aaatccgaaa agtggaaaaa
     2101 tcattaatca gcggtttaag tagcaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 acagaagttg agaagataaa ggaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattaccttt atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgatga
     2281 cttgctgcaa tttgcagcat cttatttaaa gctgccagag gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcggcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tacctcgtgt actaggagac atcatgatct ctattgaacg
     2521 tgctgctgaa caagcagaag cttacggcca ttcatatgat cgggaacttg gctttttaac
     2581 ggtacatgga ttcttgcata tcaacgggta tgaccatatg acaccagaag atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttgga tgcctatgga ctcaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaatatg aggaaacatg ttgcgttcgg tatccttgct ttatttatgg
     2821 gcgtgatttt ccagcttgat cggatagagt ggttatggct gcttttagct gtttttctcg
     2881 tttggatagt agagatcatc aatactgttt ttgaaaatgt agtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt tgctgtcatc attgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaaccga atcgtgggtc aaaaaattgc
     3181 cattatgagt gacaaggcac agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ctgattttta tcgatacacc aggaatccat aaaccaaagc atcgtctagg
     3301 agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggatgcag tcttatttat
     3361 gatcagtgct gatcaaaaac gtggccgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct cctgtttatc tgattatcaa taaaatcgat aagatccatc cagacgagtt
     3481 gctgggaatc atcgaagatt attcgacgca gatgcctttt gcacaagtga ttccgatctc
     3541 tgctacagaa ggaaacaatg tcgaacgctt aatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact tcatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggccacgat
     3781 catcgtcgaa agagatagcc aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa
     3841 gcaagtagga acgaaagcaa gaaaggatat cgaaaatctt ttaggagaca aagtatttct
     3901 agaactttgg gtcaaagtcc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 cggctaccga aaagacgagt actgatattc tggaattctt ataggttagg tatagaaata
     4021 aactggataa aaatccgtac gttgagaaaa acagcggaag atcgaaacat aaataatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcgaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaaggaatc catcggcaaa
     4261 atcagccatt agctccggct ttgctcccct ttaccgaagc agtctatatc ggtaattttc
     4321 gaagtgaagg tttgtcgttt ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc
     4381 aagaagatat ctttttgagc ggttatggta cgtatttgct aaatctagta gatgcagcaa
     4441 tcgaagatca tcaatatgac ccgcatttat tccaatttac gcaacaagct ctgcagcgga
     4501 tggaccaagg agatgatgca gaaatcatca cgaatatctt tgaagtgcaa atactgcaac
     4561 gcttcggaat cgcgccaatt tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatat ggcggtgtga tatgcgagaa acactggcca atggacgaac
     4681 accgttatca tgcagatcct cgcgctgttt atttcgttcg aatgttttcg gctatttctt
     4741 atgataagat cagtggaatc cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca
     4801 ttgaccagtt gtatgatgaa tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga cgccagagaa aaatatgcct gaaaaagaga
     4921 tacctgaaaa agaagaataa atgaaagata caacaaaaaa atcaaaacta tttgacaaat
     4981 acagggactt ccgtctatta ttaaccgtag attaaataga tcgttcactg agaggatcta
     5041 ttatacaaac cagaaaactg caaaatgaat acgtacattg accaagatca gtaaaaaaca
     5101 cgtggtttag cgagttcggg agagtggaag ccggacccaa acgatttttg aagaggcact
     5161 tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa
     5221 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
     5281 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
     5341 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
     5401 gcaagcttat gatacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
     5461 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
     5521 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
     5581 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgtcttt taggaattga
     5641 tccattagaa cacgatattc gctttgttga agacaactgg gagaacccat cgatgggttg
     5701 tgccggtgtg ggctgggaag tatggcttga tgggatggag atcacacaat tcacatactt
     5761 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagatcactt atggactaga
     5821 acgtcttgct tcttatatcc aagaagttga aagtgtttat gatctggaat gggctgatgg
     5881 cgtgaaatat ggtgagatct tcaaacaacc agaatatgaa cattctaaat attcttttga
     5941 agtaagcgac caagacctgc tactaagcaa ttttgatcgt tttgaaaaag aagctaaacg
     6001 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gtagccatac
     6061 attcaattta ttagatgctc gcggtgcagt atccgtaaca gagcgtgcag gttatctttc
     6121 ccgtatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt
     6181 aggattccct ttattgaata aaaacacagc aaatccagaa aacgtgaagg aggctgaata
     6241 agatggcaaa agacttatta cttgaaatcg ggctggaaga aatacctgct catgtagtca
     6301 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     6361 cttatgatac gatcgaaacg ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca
     6421 ttccagaacg tcaagcagac agcgaagaag aagtcaaagg accagccaaa aaaattgctt
     6481 tggacgcaga tggaaactgg acgaaagcag ctgaaggatt tgtccgcgga caaggtctga
     6541 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     6601 acactcacgg caaagaagca aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     6661 tcactttccc agtaacgatg cactgggcag aatacgattt tgaatatatt cgccctattc
     6721 actggatcgt ggcattgtta gatgacgaaa taatcccgtt tgagatcttg gatgtcaaaa
     6781 cagacagagc ttcaagaggt catcgtttct taggcgaaga tgtgacattt acgcatgcaa
     6841 aacaatacga agaaaaattg aatgaacaat ttgttattgc ttcaccgcaa aaaagaaaag
     6901 aaatgatcat cacacaagca aacgaattag cagaaaaaaa tcactggatc attgatctag
     6961 atgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc ttttctggcg
     7021 acttcgaaga aaaatatcta tctgttcctg aagaagtact agtcacttct atgaaagaac
     7081 atcaacgtta tttcgaagta cggaacgatc aaggtatgtt gcttcctcat tttatttctg
     7141 ctcgtaatgg cgatcgggta aaattggaaa atgtggtacg aggcaatcaa aaagtattga
     7201 ctgctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     7261 aatacgttga aaaattaaaa actgttactt tccatgaaaa aatcggtagc atctatgaga
     7321 aaatgcaacg ggtacaagtc atcgctcgca tcataggcga acaagtagga ctatctgagg
     7381 aagaattgat ggatctgcaa cgcgctgctg aaatctataa atttgatcta gtaaccaaca
     7441 tggtaggaga attccctgaa ctacaaggga tcatgggaga aaaatatgct ctgcttaaag
     7501 gcgagaaacc agccgtagca acagcgatcc gtgaacatta catgcctata tccagtgaag
     7561 gagatttacc tgagactaca gtaggttctg ttttagcgat tgctgataaa ttagatagta
     7621 tcttttcttt ctttgcagtt gggatgatcc caagtggctc aaacgatcca tatgctttgc
     7681 gccgtcaagc atatggtgtc gttcgaatca tcgaatcgaa aaaatgggcc tttcctttgt
     7741 ctgttctgca agaaacgatt agtgaagtta tcagtaaaga tacggatcgt tttggcattg
     7801 gcttgtcagc gggacaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     7861 tgacaacgaa gaatatccgt catgatgtca tcgaagctgt tttgaatgca gaacaaaaag
     7921 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     7981 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     8041 aaaaacaatc agaagtagcc cctgaactgt tcgaaaacga agcggagaaa gaactgcaca
     8101 aagcagtaga ggcaatgaaa acaagctttg ctgaccaaac gatcaacgaa aattatgatc
     8161 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     8221 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     8281 tgtcactagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     8341 gagaacaggc acaaaatagg ctatttc
//
LOCUS       DACCWK010000106         8321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCWK010000106 DACCWK010000000
VERSION     DACCWK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.109"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(12..689)
                     /gene="nth"
                     /locus_tag="IVW65_002284"
     CDS             complement(12..689)
                     /gene="nth"
                     /locus_tag="IVW65_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7901138.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(696..1394)
                     /locus_tag="IVW65_002285"
     CDS             complement(696..1394)
                     /locus_tag="IVW65_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7901139.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1662..2846
                     /locus_tag="IVW65_002286"
     CDS             1662..2846
                     /locus_tag="IVW65_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7901140.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2852..3295)
                     /locus_tag="IVW65_002287"
     CDS             complement(2852..3295)
                     /locus_tag="IVW65_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7901141.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3521..3808)
                     /locus_tag="IVW65_002288"
     CDS             complement(3521..3808)
                     /locus_tag="IVW65_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901142.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(3979..5331)
                     /locus_tag="IVW65_002289"
     CDS             complement(3979..5331)
                     /locus_tag="IVW65_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7901143.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5604..6050)
                     /locus_tag="IVW65_002290"
     CDS             complement(5604..6050)
                     /locus_tag="IVW65_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7901144.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6286..7212)
                     /gene="cysK"
                     /locus_tag="IVW65_002291"
     CDS             complement(6286..7212)
                     /gene="cysK"
                     /locus_tag="IVW65_002291"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7901145.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7352..8239)
                     /locus_tag="IVW65_002292"
     CDS             complement(7352..8239)
                     /locus_tag="IVW65_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7901146.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2245 a         1719 c         1395 g         2962 t
ORIGIN      
        1 agaaaccacg tctacaggcg tggtttcttt tttagcgcct tttcttttag acgcatccga
       61 tttttcccat cttgacatat tgatagcaaa ggacatacct cgcacttggg atttcttgcc
      121 gtgcaatgat atctaccaaa aaaaatcagt gtatgatgtg tctttaccca taattcttgt
      181 ggtactttcc gcatcagtgt ttcttctact tccatgaccg tagcatctag tttgcagatc
      241 ctcaggcgtt ttgagacacg ttccacgtgg gtatctactg cgattgctgg tatcccgaat
      301 gcatccccca atacgacatt tgctgtcttt ctgcctacac caggaagaga catcaattct
      361 tctcttgacg ttggcacttg cccatcgaaa cgttcaatca gctgctgtgc acacgctttg
      421 atgttttttg ctttatttcg atacaaacca atcgttttta ttttaagaat gatttcatcg
      481 attgaagctt ctgctaacgc atcgggtgtc ggaaaagagg caaacaaatc tggcgttgct
      541 ttattcactg atacatctgt cgcctgggca cttaagatca ctgcaatcaa taactcaaat
      601 ggattattgt gcttgagttc tccatgtgct tcgggaaaca ttccatacat cgtttctaga
      661 gcttccattg ttttttgttt gcttaacata ttccgctact cctccaacca atttttcagt
      721 gagactttcg gcaattcttc ttgttgagct gactgttcta cttctctttg aagaagctgc
      781 tgttttcttc gtttttggtc ttcttccact tgttgttttg tacgtagatt ttttctttcc
      841 cacgatagca aaatgcggtc aacgtaatta aaactataag cctgatttaa tacggcttcc
      901 cgcagtgcta gcttcaaaat ttccggctga taatggtctt cttctagcca ttgtccgatc
      961 cgctggaact cgatcgaaga aagcgggcgt ccaaattctt gttcaaataa ttgatacact
     1021 gactggattt ctttttcttg tgttttttct tcgtattttt tttcttgtgt ttttaaatac
     1081 tcacctaata gatcatagat ggggtaaaga ttatatcgat ctgctttttt accgttgaca
     1141 atcgtcgttt ctatcttgat aaatccattt gttacgagac gatccagtat ttgaaaaatc
     1201 tgatcttgtt tgattcccat atccatagca atcatctgca aatcagggaa agtatctcct
     1261 tcaagctgtg ccatatgcaa ctgtagaaca aataaaaatt cttcagattg tagaccgatc
     1321 ctatgataat ttttaagtaa aagattagat atgactgttt gtccagcgct taagtaattt
     1381 tctaaattca acatgatttc tcctccgctc aatcacagta tacgtaaaaa aaacatggga
     1441 agcaagaaca ggtttctttt ctatttactt cactgatcgg tcagatttac aatgtcgtct
     1501 acttgatttt tgtttgttca tgtttttgat gcgtatcttc acttttcgga ataaataaat
     1561 taagtagatt tcgctgttcc ttagaatgtt gacaaggaaa caaaattact atacactatt
     1621 tttgtttcca agttaacatt attgcataaa ggagttttta catgtacagt ttcacaaata
     1681 aacattctaa agtttttgga tacgtctctg tcttattcac tggattagga tttactatcg
     1741 tcagtcctgt acttccgttt ttaacgctgc cttattctca ctccatacat cagcaagctt
     1801 tctatatcac tttgctcatg tctgtttatg ctctagctgc cttcttatct gcccccattc
     1861 tcggtagctt aagtgatcat tttgggcgtc atccgatcct tattatcagt ttattaggat
     1921 caagtatcgg atatttgctt tttggtttag gaaattcgat atggatgttg ttccttggtc
     1981 gtatcattga aggattgacg gcaggagaaa tctccacctt atatgcttac tttgccgaca
     2041 tcacggaacc taatgaaaga accaaagttt ttggatggat gggtgcttta gtcggtatag
     2101 gaacaacgct gggtcctatt atagggggtc tattagctga gttggggaat tctgttccta
     2161 tatttattgg agcactcttt actttcttaa atgccgttta tggctacaca tttatgctag
     2221 aaagccttcc aataaaaaaa cgttcagtag atttaagttt ctcccatgta agaccttttc
     2281 accaattaca acaattattc aagatttcat ccgttattcc tttattgaca gcaggttttg
     2341 ctgtttggtt ggcagctggc tctcttcaat ctatcttttc tcaattttcg attgacgcct
     2401 ttcagtggaa ggctgggttg gttggtttaa gcttctcttt gattggaatc ttggatactg
     2461 tctcgcaatt gttcattatg cctcgattgc tcaaaaaatt ttcagaacag cagatcacaa
     2521 gaatagggat gttcagtgaa atcctcgcct atctattcat cacattatct ggaattcttc
     2581 tactgccgat tttgttttta tttgggatta tctgttatgg tttcggtgat tctattttta
     2641 ctcctgtatt caatggccaa ctatccaatt ctgtctcaga taaccagcaa ggtcttgtaa
     2701 tgggcggtac ccaaagtata caatctctat cgcgcgtcat cggcccgcta atcgcaggac
     2761 agctttatgc tgcagcacca tttcttccga cggtattcgg atttttgctt atgatttttg
     2821 cctattatca ctacaataaa aaatagtagc ttcatttgct acttcgtata aattctctgt
     2881 atcgaccgat aaaacgaaat actgtgtcaa attcttcttt tgacatattt tcaaatacag
     2941 tagcatctcg ctgtacccat ttttgatgga gttgctcgtg tctattcctt attttttgtc
     3001 cgattggcgt caattgaaaa tagatttctt ttttattctc ttcgctttga taagtgttta
     3061 tgatccctct agctatcagt ttttttgtta gcttactgat ggctcctcta gtcatgtaac
     3121 tagcattcgc taattttgtc acgttaggat cagggatttt cccgatatgt tcaatcactt
     3181 ctagttcatt tagagtaaat cctgcaaatt ctttttccat ttctggttta ttacgatgaa
     3241 taatctcatt agctaaatcc agatattctt ctattagcag ttgtctttta tccatttcta
     3301 caccttcttt ttctttcatt atattgtttt tttgtttcct attcaacaaa gataaaaata
     3361 ttttgtagga aaacccaatc tgaaaccaag aaaaaaaggg gttgacaaag cttgtcataa
     3421 cccctttttc tgattaaaag atagtaaaga gacagtcatc ggtaattgtg atgatttttt
     3481 gtcatcactt aaggatgagc ggccttttca aaaactgtct ttaaagatca tcatcagttt
     3541 ctagcacata tggctgacct tcatatgtgt actctgtatt caatgtgccg tcattttcat
     3601 ctgagtagat gcgtctactg aaaggaaaac gatgagaaac attatctttg gcgtcatttg
     3661 ctggcatcgt gtaatattcc tcttgcgttt cattcaactg agagagtact tgatctgcca
     3721 actcatcaat attgatcgtt aatttcttcg aagcactttt ataatcttct aaaaatgcat
     3781 cttgttggac aaaatattct ttattcatct tctttctcac ctttcttttc aaaatcaaat
     3841 aaagctgctt gttcttttat gtaacagctt tatctttgct gcttattgtg acaaaaaatt
     3901 cgaagaaaga caactaattg tacttttcac aaaaaaagac agagggtcgt gacaaaattg
     3961 tgtcacaacc ctttttcttt attgtgcagt ttcgatttcg cctagcgctg cttgtgcaag
     4021 taggttcaag taattccatg ggcggtcaaa atgtggttgg aagaagaaat ctgaaagtgc
     4081 gagatcttct acagtcattt tattttgaac agctaaagac atggtatttg ctgattgtgt
     4141 aatgtcgtat ttggacatga actgagcacc tacgatacga ttcgttcctt tttcatagac
     4201 tagctccatc attacttttt cagtagttgg catgaactct ggacgataat tatcttcaaa
     4261 tacagtcgct tggacttcta gatcgttcaa agttgcgctt tctttggtca cacctgtcga
     4321 accgatcgtc cagccaaata ggtaaagtcc tgaagttcct tgagtaccac gatacgccat
     4381 tttatgatct gtcaagttat ttcccacaag caaaccttga cgaacggcat ttgttgccaa
     4441 aggaatatag ttttgcgtgc cacttggatt gtagttgact actgcagagt ctccagctgc
     4501 aaaaatgtct ggattacttg tctgcatgta ttcattcact ttgattgccc cgtttggcag
     4561 tgtatctact tttccttcga tcagttttgt atttggacgg aagcccacac ataaaatgac
     4621 catatctgta tcaaatgtat ctgttggtgt agctacttgt ttcacttttc cttcttcatc
     4681 tgtgataaat tcagtaacat tttcgcctag cgcaagagta acaccgcggt ctgtcaattc
     4741 tttttctaag atatctgtga atggtttatc tagatattta ttcaagatgc gatctaatcc
     4801 atcaatcaat gtcacttgtt ttccagattc gacaaaagct tctactaatt caataccgat
     4861 atatccacca ccaacaatta ctacacgttt tgcttctttc gcttgtgcaa taatttcatt
     4921 tgcttgattg tagtttttgc ataggagtac atttttcgca tcgattccac taatcggtgg
     4981 gataataggc catgaaccag ttgtcatcac taatttatca taagaaaccg tttctgtttc
     5041 tcctgttttc agatcttttg ccgttactgt tttattttct gtatcgacgt ctgttacatc
     5101 gtgttccatt ttaactgttg ctcctaatga agataattct tcaggatttg agtagaacaa
     5161 gcctgctggg tcctttacta ctccgccgac ataaagtgcg ataccgcaag ataagaaaga
     5221 aatattgtca tttctttcat atactgtcac ttctgcttct ggatgatttt ttaaaatact
     5281 ttttacagct gcagtacctg catgagtaca gccaattact actactttca tttatggtcc
     5341 ctccgattaa cattttgtcc ttcgacaaaa ctactataac agaaataaaa cggatacaac
     5401 agtttaatgc ttgtgaattc acaaaggttt gtttgtagtt tcattccttt tcaagcataa
     5461 gaacccaata tctacaagat tcttatttta ataatattca cttttatatc taagattgtg
     5521 aataaaagac aaaaaaataa aaaagctagc gtttttttta cacagctagc tttttttagg
     5581 tataacactt gtagaaactg tttttacatt tgcgcctttt ttgctgactc tttacaatcc
     5641 ggacagagtc catacaattc caaccgatgt tcattaatct caaaatcagt cagtctgctt
     5701 gcagccattt ctatatcttc aagccctggg taatgaaaat caacaatctt accgcagttt
     5761 tcacaaatta catgataatg acgctttgaa ctgaaatcaa atcggctaga agcatcgccg
     5821 tacttcattt cttgaacaaa tccaatcgaa gtaaataagc gaaggttatt gtaaacagtc
     5881 gcaacactca tatttggaaa ccgatgttca agtgctttat aaatctcatc agccgttgga
     5941 tgggaatgat gttcaatcaa atattctaaa accgcgtacc gttgaggtgt gattcgaatg
     6001 ttctcttttt ttaacttttc gataacctct tcgactaact tattttccat gtaaacaccc
     6061 cacgttgtta ttctcgtctt atcatactat ttttacattt taaaatccac ttaacatgaa
     6121 aagaaaattc aattttattg tattttaatg aaaaaagacg taaaacagtc gcgttcacta
     6181 ttctacgtct ctattcaaat aatttttctc atcataaatt tttgtgtgtt cttttcatct
     6241 ttaaaagttt tttacataaa cctatctgga gaaaaaccaa gtgccttata tctcttggta
     6301 aagggctgta gataaataac gttcaccatt gtcgggaacc acagctaata ccttcttgcc
     6361 ttttcctaat tcttttgcga cttttagcgc ggcagcaatt gcagcgccag atgagatgcc
     6421 tactaaaatc ccttcttttc gcccaacttc tctagctgtt tccatcgctt cgtctccact
     6481 aatagaaagg actttatcat acacagaagt atccagtgtt ttaggaacaa aacctgtacc
     6541 gattccttgg attttgtgag gtcccgcttc tttcccttct aaaatagcag attctgctgg
     6601 ttctaccccg attaattcga ttttcggata cacacgtttt aattctcggc cagcgccagt
     6661 gatcgttcct cctgtaccga tacctgcaac aaacgcgtcc aagccattta ctccaaatgc
     6721 ttgatgtatc tcaggaccag tcgtcttctc atgcacaaga ggatttgctt cattttcaaa
     6781 ttgtaatggt aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc tgattgatcc
     6841 agaaatacca tccgcaccag gagtcaaaat caactctgcg ccgtaccctt tcatcaataa
     6901 acgtctttcg atactcatcg tctcaggcat cacgatgatt actttatatc cttttgcaac
     6961 tcctaccatc gacaatccga ttcctgtatt tccagaagta ggttcaatga tagtatcacc
     7021 tggtttcagt agaccatcgt gttctgcttt ttctatcata cttaaagcaa tacgatcttt
     7081 tacacttcct cctggattaa aaaactcaag ctttacaaaa acatctgctg aatcttctgg
     7141 tacgatttta tttaatttga cgattggcgt tttgccaatc aattcggtga ctgagttata
     7201 gatagctgtc atcttcaatt cctccttgtc tttgctcttc ttacttatca taatacaatc
     7261 tgttcattta tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa gatataccat
     7321 atatggtaat ttctttgcct ttttatctaa atcaaacgtt tagttcttga ccaactaata
     7381 aagaataact ggacatagac atgccatttt tcgccattag atcatcaaca gacatgccaa
     7441 atttttgtgc aatatcccat agcgtatcac ctgacacgac tgtatatgtc tgaccattat
     7501 ttgtatttac atctgttgtt gaagatgatg acgttgaagt cgtcgtagta gtcgttgttg
     7561 ttgtacttgt ggttgttgta gccgaacctg ttgatggcgt atcaaaacgg gttaaattat
     7621 aagtattaat caatgagttc aatttggccc cataatttgg atcggtagca taacgacctg
     7681 tcaagtacgc tgttgcatca taaaaacttg ttgtattgct tttccacacg ccagcataat
     7741 gataatcgcc cgtggaaaaa ctggttgaac gaagaacatt cgcatggtct tggaaggatt
     7801 cccaataaga agaatatttt cggaatggct cattcatcgt tacccattgt ccgtttacat
     7861 attctttggt tgccatataa acgacctgtc cgttatagct tcctttcaca ccgaatagat
     7921 tataatttgg cgcactagct aatccggaag tgccatatgc actttccaac aacgcttgag
     7981 cgatcatgac tgaagcatac aagtcattac ttgcagcaac ttctgacgca gaccagccga
     8041 tttgatcgat aaacgcttga ggatcagcga ctgtttgaga tccttgttca tctgcatgaa
     8101 catgttgcgg taccgctgta accatcatcg gcccaactaa aagactcgtt ccaattaaag
     8161 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgc cgggcactgc
     8221 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
     8281 tcttcttaaa acaaacttaa ttaattaaat tatactttta a
//
LOCUS       DACCWK010000107         8094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCWK010000107 DACCWK010000000
VERSION     DACCWK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.111"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            66..290
                     /locus_tag="IVW65_002293"
     CDS             66..290
                     /locus_tag="IVW65_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7901147.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(495..815)
                     /locus_tag="IVW65_002294"
     CDS             complement(495..815)
                     /locus_tag="IVW65_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7901148.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(963..3197)
                     /locus_tag="IVW65_002295"
     CDS             complement(963..3197)
                     /locus_tag="IVW65_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7901149.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3676..3942
                     /locus_tag="IVW65_002296"
     CDS             3676..3942
                     /locus_tag="IVW65_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7901150.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3942..5669
                     /gene="ptsP"
                     /locus_tag="IVW65_002297"
     CDS             3942..5669
                     /gene="ptsP"
                     /locus_tag="IVW65_002297"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7901151.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5800..6879
                     /locus_tag="IVW65_002298"
     CDS             5800..6879
                     /locus_tag="IVW65_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7901152.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            6896..>8094
                     /locus_tag="IVW65_002299"
     CDS             6896..>8094
                     /locus_tag="IVW65_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7901153.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIK"
BASE COUNT         2595 a         1442 c         1649 g         2408 t
ORIGIN      
        1 gtctaatcaa aaaaatataa aaattatttt gcgtacatat gattaaagaa aggagagtgt
       61 gacacatgga aacagaatgg caaagacgtg aaagaaaaaa acagaaaaaa gaagagatgt
      121 tacgattatg tcgattatta gatgtaaaag actgggacaa aaacgagcaa attgcacaaa
      181 aagtacgatc aatcatccat aaagaggaaa aagttaaaaa taaagacacg cgtagaactg
      241 aaaatgaaaa gagaaaagta caaaagaaaa gagaaaaatc aaaaagctag aaagaaaaag
      301 aggctgagac agaagcgttt aactccaaga aataagctgg aattcacaaa aatttgaaaa
      361 gcaattttcg tgaatttcag cttatttccg aaggagttgc ttctgctttc gccgtttata
      421 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct ttaattatta
      481 tgttcaaaat ttgtttacac ggctaatgta gcgcctaatt catgttttgg aatcgagatt
      541 tcaacaaggc ctgccaattc gattactttg atttcatcct cgtggtaaac ggcgtctttg
      601 attttatcta agaaataatt tttagcatct tcgatttctt tcggctggat tttacgtttg
      661 tcattgatca gcagaatttc attatgttca ggggcttcag tgtagacaat agtggtattt
      721 ccggctgtgt agaccttgac tagatgggca tcagtgtttt caagctggtt taatacaaga
      781 cgggaatggc tgtttgttac atttaccaat ttcatggcgc tcacctcttt tatgtatttt
      841 taattgcttg tttctcttat tataatttct tttttataaa atgaacagta ttttgacttt
      901 tctgaaaaaa gaatgagacg gcatttctca aagaaatacc gtctcacgct ataaaactgg
      961 atttattcag ccgtttcttc ggctgattct ttagccaaac gttctggttt agaagtaacg
     1021 atgatttttc catctttatc cagttttgct ttcaactcat ggatagaagg gtgatcaaga
     1081 taaaattcag cgataccatc ttcgatttgt tcttggatcg tgcgtcgaag aggacgagca
     1141 cccatagatg gatcatagcc taagtcaact aatttttctt tgacattcgt aggaacttcg
     1201 atgtgaagtt gctgtgctgc cagcatttgg ttgacatcgt ctagcattag tgtgacgatt
     1261 gtcatcaagt tttcttttga taatgcactg aattcgatga tcccgtcaaa acggttcaga
     1321 aattctggtg taaagtagtt gtttaattgg ttcaatacag aacgagtaac accttcgcgt
     1381 gccgcaccaa aaccaacgtt tgcttctact ttaccagttc cggcattact tgtcataatg
     1441 atgatcgtat ctttaaagct gaccgttctt ccttgtgcat ctgtcaatcg accatcatct
     1501 aagatttgca agaacatatg caaaacatct ggatgagctt tttctacttc atctaacaag
     1561 acaagactgt aaggatttct ccgtactttt tctgttaatt gaccagcttc ttcgtatccg
     1621 acataacctg gaggagaacc aatcaattta gagacactat gtttttccat atattcagac
     1681 atatcgaaac gaatcattga atcttgtgaa ccgaatagtt cgtaggcaag ttgtttcgca
     1741 agctctgttt tacctacacc agttggtcca acaaataaga aggagccgat tggacgattc
     1801 tgtttgttta atccaacacg gtttcggcga atcgctttcg ctactcgatc tacagcgtta
     1861 tcttgtccga tgacatgagc tttcaagtca tctgctaagt ttttcagttg tgtctgttct
     1921 ttttctttta attcacccac tggaatacct gtccgttgtt caacgatttt ttccatatct
     1981 ttttcggtaa tcacaggtgt ttcctcttca gtgagctgac gttctttcat tttttgaagt
     2041 ttattgatct gatcgcgata ataagctgct ttttcgaaat cttcttctcg agaagctaaa
     2101 actttttgtt cttctgcttc ttgcagtttt ttctcgattg ttttaggatc aacgatttgg
     2161 atcgtcaagt ttttcttaga acctgtttca tctagcaaat cgatggcttt atccggcaag
     2221 aagcgatctt ggacgtaacg attggataaa gttgcggctg cttcgatggc ctcatcagta
     2281 tattttacat gatgatagtc ttcataacgt ttttgcaaac ctttcaaaat actgatcgtt
     2341 tcttcgacag ttggttcatc tacccgtaca ggttgcatac gacgttctaa agcagcgtct
     2401 ttttcgatga tgcggtactc atttaaggta gtagcaccaa ccatttgcaa ttctcctcga
     2461 gctaaagctg gttttaaaat attaccagca tccatatttc catcaccagc agaaccagca
     2521 ccgacgattt cgtgaacttc atctatgaat aggatcacat tttctgcttg acggatctct
     2581 tcaatcaatt tttgcatacg ttcttcaaat tgtcctcgta taccagtacc ttggactaaa
     2641 gatacgacat ctaaacgaat gacttctttg tccattaatt tttgcggtac atcaccatct
     2701 acgatttttt gtgctaaacc ttcaacaact gctgttttac caacacctgg ttctccaatc
     2761 aagacaggat tgttcttggt tcggcgattt aaaatttcaa tgactcgttt gatttcgtca
     2821 tcgcgtccga tcacaggatc gatatcacct tgtcgtgctt gttctgtgat attgattccg
     2881 tattctccca ataaaccttc tgtattgcct tgaggtggct gaccaccatt gaagttgttg
     2941 tttccgccaa attgtgttgg tggtgtttgc tcataatttc cttgctgttg catttggcga
     3001 gacatggaac ggaaaagatc gtccaaactg ccaaatccaa atggatcatt ttgggccata
     3061 ttagtcagac ctccattttg ttgatttttg attttttgat aacagctctg acagtaatcg
     3121 agttgtgtcc gctgaccatt gacagtggca tacaagtgaa tcgttgcctc attcttgccg
     3181 cagttttgac aaagcatgaa tcattcacgt cctttcacaa aaatgggtac atccatattt
     3241 tacatctcgt ttttcatgag gtaaaagaat aacccctagt tttttaagag atttatgagg
     3301 tagaaaaggt gaaatctcta ttgaccattc ctgacctttg actcttatta tactaaaaat
     3361 aaaaagggaa gcaagtaatt cgtttcatat gaaaacaaag agggtcaaag gaaagaatga
     3421 taaacgtcag gagaagagaa agcgacaaga aaaaggaaat aaacacgttt gttataaaat
     3481 gtgaagaaaa ggatgaaaaa gaatccggaa taatttgaat acttttatct gaaattgaat
     3541 gaaaactgtt gtatctaacg aaaaaaaatg ttaatcttta cagatgaaag ggtggcattt
     3601 gttaggattc tctgaattac taaaaaagaa aaccctcaca taaaaacaac actcacttaa
     3661 aggagaccga ttaatatgga aaagaaagaa tttcacgtag tagcagaaac tgggatccac
     3721 gctcgtccag ctacactatt agtacaaaca gcaagcaaat ttaactctga tgtaaactta
     3781 gagtataaag gtaaatcagt aaacttgaaa tcaatcatgg gcgttatgtc tttaggtgta
     3841 ggtcaaggtt ctgatgttac tattactgca gaaggtgctg acgaagctga tgcaatggca
     3901 gcaatcgttg aaacaatgaa gaaagaaggc ttatctgaat aatggttgaa atgctaaaag
     3961 ggattgccgc tagtgacggt gtagccgttg caaaagctta cctgctagtt caaccggatt
     4021 taacattcag taaggcaaca gtagaagata ctgcggctga agaagctcgt ttggatgctg
     4081 cattagctaa atctactgaa gaattgcagc aaattcgtga aaaagcagcg caaagcttag
     4141 gtgaagcaga agcgcaagta tttgatgccc atttgatggt tctttcagat ccagaaatgg
     4201 taggtcaaat caagcaaaat attaaagaca acagcgtaaa tgctgaatca gcgctaaaag
     4261 aagtaacgga tatgtatatc ggtatgttcg aagcaatgga agataatgct tatatgcaag
     4321 agcgtgctgc agatattcgt gacgttgcta aacgtatttt agcgcactta ttaggcgtta
     4381 ctttaccaaa tccatcaatg atcaacgaag aagtggtcgt tgttgcacat gacttgactc
     4441 caagtgatac agcacaatta gatcgcaatt ttgtaaaagc gtttgtaaca gacattggtg
     4501 gacgtacttc acattctgct atcatggcac gttctttaga aattccagca atcgttggga
     4561 caaaagaaat tactgctaaa gtcaaagaag gcgatatttt ggctgtcaac gggatcgaag
     4621 gcgatgtaat catcgatcct acagatgaac aaaaagctga atttgaaaaa gcaggtgcag
     4681 attacgcagc acaaaaagca gaatgggaaa aattaaaaaa tgcagaaact gtcacagctg
     4741 acggcaagca ttttgaatta gctgcaaaca tcggaacacc aaaagatcta gttggtgtcc
     4801 ataataatgg tggagaagca gttggtctgt accgtactga attcctgtat atggattctc
     4861 cagacttccc aacagaagat gaccaatatg aagcttacaa agctgtatta gaaggtatgg
     4921 aaggaaaacc tgtagttgtt cgtacaatgg acatcggtgg agataaagaa ctaccatatc
     4981 tgcaattacc tcatgaaatg aatccattct taggatatcg tgcattgcgt atcagtttat
     5041 ctgaacaagg cgacgaaatg ttccgtacac aaatgcgtgc attgcttcgt gcatctgttc
     5101 atggtaattt acgtatcatg ttccctatgg tagctacact caaagaattc cgcgcagcaa
     5161 aagcgatctt tgaagaagaa aaacaaaaat tgataagtga aggcaaggaa gtatctgata
     5221 cgatccaagt cggcatcatg atcgagattc cagcagcagc agtcttagca gacaagtttg
     5281 caaaagaagt agatttcttc tctgtaggta cgaacgattt gattcaatac acaatggcag
     5341 cagaccgtat gaacgaacgc gtttcttact tgtaccaacc atataaccca tcaattttac
     5401 gtttgatcaa aaatgtaatt gatgcagcac acgctgaagg aaaatgggca ggtatgtgtg
     5461 gagaaatggc tggagatcaa acagctgttc cattgctagt aggtatgggt ctagatgagt
     5521 tctcaatgag tgcgacatct attcttaaaa cgcgtagttt aatgaaacgc ttggacacag
     5581 cgaaaatggc tgaacttgct gatcgtgcat tgaaagaatg cgatatgatg gaagaagtcg
     5641 ttgaacttgt tcatgaatat gtaaaataaa caacgtatca aaagctggtg tggcttgtaa
     5701 aagagctacg ccagtttttt tgttcttttt taagaaaacg tttttgtcag taagaataga
     5761 caaaaatagg gcttaagttt gagtgacagt ttgtaggcta tgttatacta tataagtgaa
     5821 gggggacgta aggtgaaggt attattatat tttgaaagtg aaaagatgct tgcaaaatct
     5881 ggaatcgggc gtgcgcttga tcatcaaaaa cgtgctctga cggaagtcgg tatctcttat
     5941 acactagatg aaaaagaaga ttatgacatc ctgcacatca atacatacgg aatcaatagt
     6001 cataatatgg tcaataaagc cagaagagaa ggaaaaaaag ttgtctatca tgcgcattct
     6061 acagaggaag atttccgaaa ttcctttatc ggatcgaacc agctttctcc tatcgtaaaa
     6121 aaatatctgg taggattgta ccaaaaagcg gactatctga tcacgccaac accttattca
     6181 aaacagttgt tggaaagcta tggtatccgt gtgccgattc aagctatttc caatgggatc
     6241 gacttagaga aatatcaccc tgatccaatg aaagaacaaa agttcagaga atatttcaaa
     6301 ctgtctccgg atcaaaaagt aatcatttgt gtcggtttgt ttttcgaacg taaaggaatc
     6361 attgatttcg tagaaattgc taagaagatg cccgaataca catttatttg gtttggtcat
     6421 gttcccatgt actcgattcc tcggaacatc cggaaaattg taaaagaaga tcatcctgaa
     6481 aatgtcctgt ttcctggtta cattcgtgga gagatcatag aaggtgcgta ttcaggtgca
     6541 gatctattct ttttcccttc ttatgaagag acagaaggga tcgttgtttt agaagcttta
     6601 gcaagcaaac aaaacgtact tgttcgagat attccagtgt ataatggctg gttagaagat
     6661 agcaagaact gctatatggg gaagacaaac gaagaattta tccgactgat ccaacagatt
     6721 accaatcaag aactaccgag tacgacacaa gccggttatc aaacagcaaa agagcgaagt
     6781 atcaataaaa tcggtgaaga acttaaaaat gtatacgaaa gtgtactgaa tgaaaaagtc
     6841 tctagcaaat taaaaaaagt aaatcaggtc aaggactagt ttggaggcga aaaacatgaa
     6901 aatcggcttt tttaccgata cctactttcc gcaagtcagc ggagttgcta catcgatcaa
     6961 aacattaaaa caagaattag aaagacatgg gcatgaagtc tatattttta caacgaccga
     7021 tcctaatgcg gataagttgg aaaaagatgt gattcgaatg ccaagtgtac catttatttc
     7081 tttcaaggat cgaagagttg ttgttagagg catgtggtat gcttatttgg ttgccaaaga
     7141 actagaacta gatcttattc atacccatac agaattcgga gcagggatac ttgggaagat
     7201 ggtagctaag aagttgaaaa tacctctgat ccatacttac catacgatgt atgaagacta
     7261 ccttcattat atcggtaaag gaaaagtgat caggcaatcc catgtaaaat acttgtctag
     7321 attatttgca aatcatacga caggtgtagt ttgtccaagt gaacgtgtta ttgatacatt
     7381 acgcagctat ggtgtaatag caccgcttcg cgtgattcct actggtatcg acattgaaaa
     7441 attcaaacgt tctgatatca ctcagcagga tatcagcaaa ttaagggagt cactaggtct
     7501 tcaggaaaac catttgatga ttctttcttt aagcaggatt tcttatgaga aaaatatcca
     7561 agccttgatt aatgggtttc ctcaggtcat ctccgcttat cctaacgcca gattagttat
     7621 cgtaggaaaa ggaccttatg tagaagagtt agaggaattg atctcagaac tgcagttaga
     7681 agaatttgtg cagttcacag gagaagtgga caacaatgat gtagcacttt attataaagc
     7741 ggcagattat tttgtcagtg cttctacttc agaaacacaa gggcttactt atacagaagc
     7801 gatggcagcc ggaacacctt gtgtcgtgga aggaaatgct tatttgaacc gattgttcga
     7861 tcatccttca ttagggaaaa cttttgaaac cgacgaagac tttgcaccag cacttattga
     7921 ttatatttcc agtgaagttc ctaaagatcc aaaagtttta gaagctaaac tttatgatat
     7981 ttcatctaca catttcggcg aagctatgat cgaattttat caagacatgg ttgtttatca
     8041 tgaagaagag atgcgaagaa aagaagaaga agactcgatt gaacgaatca aaat
//
LOCUS       DACCWK010000108         7893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCWK010000108 DACCWK010000000
VERSION     DACCWK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.112"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..320)
                     /locus_tag="IVW65_002300"
     CDS             complement(27..320)
                     /locus_tag="IVW65_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7901154.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(348..1013)
                     /locus_tag="IVW65_002301"
     CDS             complement(348..1013)
                     /locus_tag="IVW65_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7901155.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1153..1785)
                     /locus_tag="IVW65_002302"
     CDS             complement(1153..1785)
                     /locus_tag="IVW65_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7901156.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1792..2859)
                     /locus_tag="IVW65_002303"
     CDS             complement(1792..2859)
                     /locus_tag="IVW65_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7901157.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2856..3587)
                     /locus_tag="IVW65_002304"
     CDS             complement(2856..3587)
                     /locus_tag="IVW65_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7901158.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3755..4234)
                     /gene="greA"
                     /locus_tag="IVW65_002305"
     CDS             complement(3755..4234)
                     /gene="greA"
                     /locus_tag="IVW65_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7901159.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4370..5545)
                     /gene="mltG"
                     /locus_tag="IVW65_002306"
     CDS             complement(4370..5545)
                     /gene="mltG"
                     /locus_tag="IVW65_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7901160.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5710..6870
                     /locus_tag="IVW65_002307"
     CDS             5710..6870
                     /locus_tag="IVW65_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7901161.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6910..7857)
                     /locus_tag="IVW65_002308"
     CDS             complement(6910..7857)
                     /locus_tag="IVW65_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7901162.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
BASE COUNT         2337 a         1672 c         1229 g         2655 t
ORIGIN      
        1 caaaacttgt gtcaccctct ctttttttat tgttttttaa agtgataggt tggctcctcc
       61 aggttgggat cataatcaac aagtaaatca tatcctttaa agaaccattc atctgcttcc
      121 tcgataaaaa aaagtgtccc atccatttcc actttcttta caggatgttc tggctgttca
      181 actgacattc caatcgaaaa accatcatga acctctgttt tgccgtagac ttttccaaaa
      241 aaacgtattc cgtaagtttc tggcagatca atttctgagt caaaccactt ttgtgctttt
      301 ggcgttaccg ttaattccat cccaacaacc tactttctct tttctttcta ttgattcatc
      361 ttatcgtcta gaatatccac atcgcctgtc tctccaatca ccagtagatt atcattttcg
      421 cgaacatatt catctgctgc aggagaagcg ataaccgttt gatctactct tcgtatggcg
      481 actactgtta agtcaaaacg ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat
      541 ttaggattgg agactcggat ttctgctaaa gaatatttat ctgacagctc taagtagtct
      601 agaatattct ttgatactaa gttatgtgca atccgtatcc ccatgtctcg ctcagggtgt
      661 acgacacgat ctgctccgat cttctctagt acacgggcat ggtactcatt ttgggctttt
      721 gctaaaatat tcggtacgcc catttccttt accatcagag taactaagat actggcttgg
      781 atatcttctc caatagcgac aattacatga tcgaaatttc ttatacctaa tgaacgaagt
      841 gtcatctcat cctgtgcatt tgccacaaca gcatgagtgg ctatattcat atattcattg
      901 actcgatctt cattactgtc aatagccagt acttcttgtc cagcttcgat cagtgtacgg
      961 cagatgcttc cgccaaatcg tccaaggcct acaattgcaa aattttgttt caccattttc
     1021 ctcctttagt atcttctttt ctcagtataa caaagccttt ccaaaaacaa aagcaagcat
     1081 cattattgta aaaaagccaa gaaaaataat agttattttc tcagcttttg attatcctct
     1141 ttatcatcgt ttttatttca ccaaaccatg tttaaacgca tagatagctg cttgagttcg
     1201 gtcttctacc tctaacttag ctaatatatt cgacacatgc gttttaactg ttttcagtgt
     1261 gataaacaat tcatccgcga tttcctgatt acttttccct tcagaaatca gcatcaaaat
     1321 ttcattttct cgattcgtta attcttcatg caatactgga tcatttcgcc gactcatttt
     1381 ttccatcatt tttgttgtca cttcaggttc caatactcgc tcgccgcgtt gcgtagccct
     1441 tatagcatca gctatttcat gtgcggtaga cgtcttcaac aaatatcctg ctgctcctgc
     1501 ttcaattgca ggataaactt tttcatcatc aataaaactt gtgacgataa tgattttggc
     1561 ttttggacaa tcttttaaga ttgcttttgt tgattcgatg ccatccattt cttccatgac
     1621 tagatccatt aaaatgacat ctggtcgaag tgccatcgcc ttttcatagc cttgacgtcc
     1681 attttcagct tctccaatca cttcaatgtc ttcttggata gataaataag aagagacgcc
     1741 taagcgtacc atttcatggt catctactaa taaaactttt atcatcttta tctatgcctc
     1801 cttcatcaaa gggaccttga tctccacgct cgtaccttgc cctttaaaac taatgatttt
     1861 tactgttcca ccaataccag ctactcgttc tttgatattg ttcaatccat aacttcccgt
     1921 tttggtttca ttcatattaa atcctgtccc gtcgtcaatg atacgtaata aaagattgtt
     1981 atctatttta tgcaaataga cttctaattc attagcctta gcatgtctta atgtgtttga
     2041 taacaattct tgaacgattc ggaacaagtg atcctcaatg gaactagtta attttacatc
     2101 ttctacatcc cattttagcg aaatctggat tttattctgc aattccttca ataactgctc
     2161 aatgccttgt tttaaacttt tttcttctaa attgactgga cgcagatgaa gaagcagcgc
     2221 acgcatctca gactgggaag cgttgatgat ctctgccacc attttcaact gcttacggaa
     2281 catttctggt gtctcacttt tttctgcttg ctccgtcaat gccgacatca tcatcatcgc
     2341 tgcaaataat tgctgtgaaa ctgaatcatg aagctcacga gctaatcgat gcctttctaa
     2401 ttctaaaatc tcttctttgg tttgtccatc tacatattga ggacggctag tcataatctg
     2461 taattcactg gatacttcga tcattttttc ttttatttta gcaatttcca tatcaatatc
     2521 tttgatgtac agatcatccg acgcgcttgg gatcggctga tgcaaaagtt tggattcata
     2581 attgccagaa gaaagtgcac gaagcttttc ttcgattttc ccgtattttg ccttttgaat
     2641 aagtgataac aatagaaata cgatcaatcc tacacctaat gaaattgcca tcaaatggaa
     2701 gataagcgga acgtataata aacgcgcacg cagcaattct ccccaccaat gactttggtt
     2761 actggcataa aaataagtga aaagtgaaaa tagaataata aggaaagccg caatccccga
     2821 ataaacagct agcattgctt tggatattct tcccatcata ttcgtatcac ctcgacatct
     2881 ccaaccaagg tattggtaat gatcttcaat ctgcgaggat tttcatcata atcactgctg
     2941 cagattttga tctgttcgtt ttttaaggaa aactgctctt cttcaaacaa gacatttcct
     3001 actaatgtcg catgctctaa acgaatagcc actcccaggg gtactaagat ccgtgttctg
     3061 cctatccctt ttcttacaat cacaatatta tcatcttttg gcaaaagtgt attacccaaa
     3121 tcgatgatag tatcaccaga aatgatggca atgttgatat catcccattc atagacgtcg
     3181 tttccaatac gttggttacc aaataattgc tgtcttttac gctcattatt atgtgttttt
     3241 aattgctctg tttggaccat catgatctgt ttttttcgcc aaaaagcatt ttttgtcaaa
     3301 tctattcctg aaatctctac acctttcagc ccgataaata aaacggccaa tacaaccatc
     3361 atccataaag caggactgtt gactaagcta aaaaaaatga ccaagctccc taagatcaat
     3421 tgaaaatttt gaaatttagt acgaggatat ttgcgcatag ctagatatat attgaaaata
     3481 ccgaatatta ctaaaagcaa aagtccagta ttgtttacga tttgccaaac agcaaataac
     3541 agcagcagtg cttcgactac cacaaaaaaa cgccaagaac tattcatcgc tttttctccc
     3601 tcctgacaca ttctcatttc atcataccta tttttttgac tgttggctat aggactttag
     3661 atgtaagatc gttcagtgaa acctgcaata ctcaaagaga aaagatctag agtgcgtcat
     3721 cagtaagatg aactctctag atctttcagt cagactacgc ttgttctact ttgacgattt
     3781 taactaacat atcgccacct ggtgtagcaa tggttacttc atcattttta cgtttgccaa
     3841 tcaatgcttg cgcgatagga gaatcattgg aaatctttcc agaaaacggg tctgcttcag
     3901 cacttccgac aatcgtgtac tcttcttcat ctccatcagg caactcgatg aacgtaactg
     3961 ttttaccgat cgatacctca tctgcatcta cgccgtcatt atcgatgatt tgtgcaaaac
     4021 ggatcatatt ttcaattgtt gtgattcttc cttcaacaaa tgcttgttca tctttagctg
     4081 attcgtattc ggagttttcg gaaagatctc cgaaaccgcg agcgattttg attcgttcaa
     4141 tgatttctcc gcgtttaact gtttttagtt cttctaattc tttttctaat ttttctttac
     4201 cttctagagt cattggataa actttctcta ccatgtgtaa cgtctccttt tttcgataaa
     4261 ataccgcaaa aggtaatctt ttgaggaata tagtccaaaa aagattacca cgcttttctt
     4321 aaaatgtaaa tgtttttcta aaaatgatca atgactagcc cctgtttttc taagaattat
     4381 ttacatattt ttgcactagt tccatatgtt cttcatacgt tttagcgaaa taaacatttc
     4441 cagtgctcgt atctgctacg aaataataat agtcattttc tgctggttct aatactgcct
     4501 tgattgcctt ttcgcttgga ttgtcaaaag gaccaggccc atagccggta ttggtataca
     4561 aattgtatgg tgaatctaca gctgtatctt catacgtcac caattcttta tgttctccta
     4621 atgcatataa aacagagata tctgactgaa gtggcatatt tgccttgatt cggttaaaga
     4681 agacttgtgc aatattttta cgatcatttt cttttacgcc ttctttttca actagagaag
     4741 ccaaagttaa aacttcttgt acagtcaaat ttttctgttt gatagctgaa taatagtttt
     4801 gcatgaccgt atttgtcttg ttcaccatct caatcacaag atccttaagc gaagtactcg
     4861 tgtagtaatc ataagtagct gggaataagt agccttccaa acggtatttt gtgttctcag
     4921 ctttagaggc actttccaac aattctggga atttttgata caactcgtta aagaaagtct
     4981 catcatttac taaatctaaa aattctttct tgtctttacc tgttaccttc gctacccgct
     5041 cagcaatctg atcgatgtcg tagccttctg gaatggcaat tttcccatct gcaacttttg
     5101 ttggctcaga tgtgccgcct tcttgcaact gcttgccaat ctcatcaaga gtcatattag
     5161 gcgctaactg ataataacca gcttgaaagc cagttaagtt tttaaatttt gtataataat
     5221 tgaaaacaat cccactttta ataatattgt ctttctccaa aatttcgcca atctgcttat
     5281 tagaagagcc gtttgggatt tccacttgga ctaattggtc gtccgattta tctaaaggct
     5341 tcaacccgct atctacataa cgatatacgg taaacccgag aaatccgcca ataatcaata
     5401 atgtaagagc gatcactaaa atgatcttcc tcacgatctt atcttctttt ttacgcagat
     5461 ttctccgctt gtcgattata tctgctgtag ctgccttact ggcttctttt ttgtctttgc
     5521 ttgggtgttg tttgtttcct gacaaaacaa ctcctcctta ctatcatctt cgtaactctg
     5581 tttattatac atgaaatttc tactgccgtg aattatttta ccaaatatta tgtcaaaatt
     5641 gtaagtggca aatgctcttt ttgaggatta caatgaaaaa tagttaaaaa tggatagaaa
     5701 ggatttaata tggcaaaagg aaaacatcaa aaaaaacagc agatctctct ttcttttctc
     5761 gcagttttgt tgctggctgt cagcttcttt tcaacaggca tcattacagg atggcaatta
     5821 acaaaacaac ctcaaccaca aatttataaa acggaacccg cacaacttgc ttccaattta
     5881 acatctaatg agcagatgct tgccagcaaa attgatcaat tactgcaaaa atcagattat
     5941 atcggttcga tttatgtacg caaaaataat cgagtgatat tagaaaaagg atatgggtac
     6001 gcaaatctac gcataaaaga atcgaattct ccttctcttt actatcaaat cggttcgatc
     6061 caaaaagcga tgactgcttt acttatccta aaacaaattc aatctggcaa gataactttt
     6121 gacacaaagc tatctgaatt ttatcctcag attcctggaa gtcagcaaat ctcaatcaaa
     6181 aatatgcttt acatgcgctc tggtcttcat cgaaccgctt ctccaaaaaa acctatgtca
     6241 gatagtgaga tcattcagtt tgcccttcat catttgaaat ttactggtta tgatacgtat
     6301 cattatgaac cattaaatta tacattgctt acaggggtat tgatcaaact aatgaatcag
     6361 ccttatgaaa ctcttttaaa aaaagaaatc attcggccac tccatctaga acatacagat
     6421 ttttatgaaa atgtaaaaaa ttcccctcag catgctgtat cttaccaaat gtctgcaact
     6481 gacgattatt caaaagcatt ggtcgagcct gaaacagaca tccgaaatga attaggcaca
     6541 ggaaatataa gtatgtctgt ttatgacttg aacaaattct ttaccagtgt tctgtctggc
     6601 gatattatac caaaagaact acttttctca ttgtggcaaa ataatggtga taaacatcct
     6661 tacagcggcg gtgtttacag tggaaatgat tatatccttg ctcagggaaa tatcaatcga
     6721 ttccatgcag tagcggcgat gaaaaaagac acaaaagatg caatcgtcat ggaaagtaat
     6781 atccaatctg ataaaaacat caaattgccg gccactgatt taagaaatca gatttacgaa
     6841 ctgatggaag gtgttaattt aaaaagctaa gtcgagacaa aaaaaccagc aggatgctgg
     6901 ttttttgttt caatcgattt ctgtctttaa ataacgttcc attccgttca atatatcaaa
     6961 tttgggttga taacctaaag aacggatctt tgaaatatcc gctaaagaat cacgaatatc
     7021 ccctgaacgc tccgcttcat atttaagcgc tagctctgtt cctaagattt gatcaattga
     7081 atgaatcaat tcaagtagcg tagtgctttt tcctgtaccg acattgaatt gctgtcctaa
     7141 agctttttct tccttagcaa caagtaaaag tgcttgtata acatcttcta tataaacgaa
     7201 gtctctagat tggctaccat caccaaataa agtaaattct gttttttctc ctgccaattg
     7261 ttttttgtat cggtccacta gaatcgaaat aactccgcta tatggagagt taggattttg
     7321 atttggacca taaacgttaa aaaatcggac agcactagtc ggaacatcat ataaatgaca
     7381 gtaatccaaa acatattgtt ctgatgcaaa cttatcgatg gcatatggtg ttaaaggacg
     7441 gatgaccgat tcttcttttt ttggcaacgt tggttcatcg ccataaactg cagctgatga
     7501 actaaatacc aatcgtttta aaccagattg atattttcgt accaattcaa gcaataaaag
     7561 cgcactgtca aaatttacac gatgagtttc tagaggacgt tctacagaat ctgccacact
     7621 agctacagca gctaaatgaa aaatataggc aaactgatac tgtttcatga ttctttccat
     7681 caattgagga tctgatacat ccccctcaat gaaagtcact tgtttctcca tatccaagtt
     7741 ttcttttttt cccattgata aatcgtcgac tactacgatt ttgttttctt ttcccaaata
     7801 attcgctagc gttgaaccga taaaaccagc tccaccagta atcaatatag tatccatcaa
     7861 attcactcca attatgcatt ctcatactta ata
//
LOCUS       DACCWK010000109         7851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCWK010000109 DACCWK010000000
VERSION     DACCWK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.113"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..786
                     /locus_tag="IVW65_002309"
     CDS             <1..786
                     /locus_tag="IVW65_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall surface anchor protein"
                     /protein_id="HAP7901163.1"
                     /translation="YVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQ
                     VSDTVANLHGQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVV
                     TYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDV
                     ANAAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRIT
                     IKNNQKASVPMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            799..1551
                     /locus_tag="IVW65_002310"
     CDS             799..1551
                     /locus_tag="IVW65_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7901164.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            1567..2328
                     /locus_tag="IVW65_002311"
     CDS             1567..2328
                     /locus_tag="IVW65_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901165.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            2341..2601
                     /locus_tag="IVW65_002312"
     CDS             2341..2601
                     /locus_tag="IVW65_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901166.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            2598..4688
                     /locus_tag="IVW65_002313"
     CDS             2598..4688
                     /locus_tag="IVW65_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7901167.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(4724..6781)
                     /locus_tag="IVW65_002314"
     CDS             complement(4724..6781)
                     /locus_tag="IVW65_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7901168.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(6926..7117)
                     /locus_tag="IVW65_002315"
     CDS             complement(6926..7117)
                     /locus_tag="IVW65_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901169.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            7305..7760
                     /locus_tag="IVW65_002316"
     CDS             7305..7760
                     /locus_tag="IVW65_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901170.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            7757..>7851
                     /locus_tag="IVW65_002317"
     CDS             7757..>7851
                     /locus_tag="IVW65_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901171.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILER"
BASE COUNT         2664 a         1296 c         1540 g         2351 t
ORIGIN      
        1 tatgttgctg aaaaagcaga tattaaagta acaaataatg atggttcagt cgtattaaaa
       61 gaaaatactg actataaaat cgaaaattct gatagtggtt ttgtgatcga ttttatcgta
      121 aataatggac aagtaagtga tacagttgcg aatttacatg gtcaagatct aaaaatgact
      181 tataacatgt ttgtgaatga tagtgctgaa atcgcaaatc cattaacaaa tagcgtggat
      241 ttcgtttata acaataatcc atttaatcag gaagaacacc acgaaaaaac gaaagctgac
      301 gttgtgactt atggcgcaaa attcttaaaa gtcgatagcg gcctatttgg aacaggtatc
      361 aaagcaacac cgcttgaagg cgcagaattt gcagctaaga atgcagaagg taagtactac
      421 ggtggactta ctgatacaga taaagatggt gtcaaagaag cagtttgggt agacgatgta
      481 gcaaatgcag caatcctaaa atcagataaa gagggtcatt ttgaaatcac tggtttaact
      541 gaaggagatt actcactgga agaaacaaaa gctcctgaaa actatcaaaa attaacaaaa
      601 gagatctctt tcaaagtaga caaagattca tacaaagaag aaaaccgaat cacgatcaaa
      661 aataatcaaa aagcttccgt accaatgact ggtagcaacg gtttccaatc ttacgtattg
      721 atctcttgct tgttgcttgg ggcaggcgca atttctgcag ttgtttactt taagaagaaa
      781 gcctagaagg atcagctaat gattatcaga caccctaaga aaaagcgaat catgggtaaa
      841 tggatcattg ctttctggtt actttcagct gttggtgtct tacttttgat gcctgctgaa
      901 gcgagcgtgg cgaaatacca acaaaatcag cagattgccg caatcgatcg aacaggaacg
      961 gcagcagaaa cggattcttc tttggatgtt gctaagattg agttaggcga tcctgttggt
     1021 attcttacaa ttccaagtat ttctttaaaa ctaccaattt atgacggaac ttccgacaaa
     1081 attttagaga atggtgtagg gattacggaa ggaactggcg atattacagg aggaaacggt
     1141 aaaaatcctt taatcgcagg gcacagtgga ctttataaag ataatctttt cgatgatttg
     1201 ccttcagtta aaaagggaga aaagttttat atcaaagttg atggtgagca gcacgcttat
     1261 caaatcgatc ggattgagga agtgcaaaaa gacgaactac aaagaaactt tgtcacttac
     1321 ttagaaccaa atcccaatga agatcgagta acgctaatga catgtacgcc taaggggatc
     1381 aacacacacc gtttcctagt gtatgggaag cgagtaacct ttacaaaatc agaattaaaa
     1441 gatgaagaaa ataagaaaca aaaattatct tggaaatggc ttttaggaag tactgttttt
     1501 ctttcagtca tgatcattgg ttcgctattt gtttataaaa agaaaaaata aaggagggta
     1561 accaacatga gaaatagaaa atggctattt gtttgtgcag ccgctctttt ttctttcact
     1621 gcctacacac agttgacgtt tgcagacaga caagaaagca cggatagcac agaacaaaca
     1681 atccaaacgt cagatagcag cacgattcaa agcagcgatg aaaccgtcga ttcgtcaaca
     1741 gttgattctt cagaagaacc taaaattgct gaagacgatc aaatagtcaa acagaccatt
     1801 cacgttcaaa aaattatttc ttccgaacaa ttagacaacc aggggcaaaa gcttgaaaaa
     1861 caagaaggag taaatggcgc gaagttttct gtttatgatg tatcggacat tttacaaaag
     1921 atggatgtta aggatctcac tactgaccaa attgaatccc aattaaaaga tcgagtgaaa
     1981 aaactatcca gtgatcaact gaagctaatt tctaatggag aaacaaagac aattgatcaa
     2041 caaacaggtg tctttgaatt ctcggtagaa gtacaggcaa atcagaaaca agcttattat
     2101 attgtcaatg aatcaagtcc tgaaaacatt tctaatagcg aggatatttt gctattaaca
     2161 cctgtttctg ataaaaatgg tgaattctta aaggatgtgt ggatttatcc taaatcagaa
     2221 gcttcgcaac ctaaagaaga agtaaaaaaa atcgtatcta ctggcgtaaa gaaaaacttc
     2281 tttgagaatt gttgggattt tgttactcac ttgttttcta gagattaagg aagaaataaa
     2341 atgaaactga tcaaattatt attattcaat ttttcttgtt taaccgcagt ctttttgtac
     2401 tgcggtttat tattttctaa actatctatt ttgccaagct accaactata cctaatgagg
     2461 attttaaaca taccagctgc ttccatgccg ctagatatta tggaaaagct cattaggaat
     2521 ccattattta ctacgatcaa tttgatcgtt attgtcggaa tttcactagt catttataaa
     2581 aaagagggga tgaagggatg aattttaaac actcgtggac aagacggtta aaatatgttt
     2641 ttcccataat gatgatgtta ggtacattgt ttgggttaaa aacagccaat gtctttgctg
     2701 aaaaagttat tgtcgatccg tcaaatccaa tccagaacgt aaagaataat tttattattc
     2761 caaaagatgt tcctaatgca aaaacaaata ttactgtaaa cggagcgtca aaagtttaca
     2821 aatatcatgt tgatgccaac aaaggtggtt ttacagacga ctacgtagga ttagtggagg
     2881 gaaacaaaaa tatacgctat ccttctttcc aaaaagaata cggtgaaaca aaccttgttt
     2941 tagaaggcgt aacaaccaac accaaagtca atattgacta tggaaaaatc ggtacgtata
     3001 acgggaaaaa agtcaatatc aagctagttc tatctaatat tcacttatac tcggatacgt
     3061 tgccgtggaa tctactagat aacaactata caaaaactca ttttcgagac gatggctata
     3121 aaaatacgaa tggtgcaatg agtaaatcta aaaaaagaac agtactttgg atctcagaca
     3181 atcttttctc aggaattgtt taccattcaa cacaaatgaa tgtgcagcta gtagccacat
     3241 atgaggacgg tagccccgtt caattttcag gagatacatt tatcagtttt aactctttga
     3301 accctgcagg aggaaaaagc acggatctta aaggagaata tgcccactat gacaaaatga
     3361 atacgacaga ttggtatgtc agaaaagata cggttctttc agaatttaaa tctttctaca
     3421 acaatctgaa cgtggtaggt ggccacccag gaggaagctc taagctgaca caagcggata
     3481 atgatttcaa taatttgcat gataaattag gagatccgaa gtttggtcaa ggtactgtgt
     3541 cctttaaaat atctgaagca aatccgacgt ttgtgattgg ttcttctaat gttcaaacct
     3601 ggtttacttt atcttctgcg acaatcttta gtgttgttcc tgatcaacct gaaaaaacag
     3661 gggttgataa aaatggaaat aacgtgaacg ataaaatgtt gcaagtagga gacacgattc
     3721 aatatcgaat caaacagaag gtgaatcgat taggagtgga tttactagcg gtttacgatc
     3781 gatttgagtt aatcgataat ttaccaaaag aagtaaatta cgtcgatgca cacgttgaaa
     3841 gtggtacaaa taaaaaattc gatgtatctg gtgaagtgac ctatgacaaa acaaaacacc
     3901 aggtaaaata tgcagcgaaa gctgatacat taaaaaatcg aatgaagtat aacggcgaaa
     3961 cttacgaatt agtgatcaat gtaaaagtca atgaattggc caatcaaaat agtgtagcca
     4021 aaaatcaggg aacgtcgatc attaataaag tagaaaagga tacaaatatc attaccgttt
     4081 atttccctaa aattccagtg aaagaagtac aacaaaatgg aaaagatgta aacggacgaa
     4141 atgacggtaa gaagggtacg ccgacagctc ctttaaacgc aggatctgaa gtacaatatc
     4201 tagtcacaca aaaatggcac acaaaaggcg tagacgctgc gtcagatcac tacaaacaat
     4261 ttagtattca agatccaatt gaagctcgtt tgacatataa agaaggttct gcgcaagtaa
     4321 tagataagag taaaggaaaa gatattactt ctgaaggcac actaacctat gatagcaatt
     4381 caagaacgct gaagtgggaa gcttccgctg acttcttaaa taacaatctt ttagatggac
     4441 gagaaattca gctgatattt acagctaaaa ctccattaca atcagaaaaa aatattgata
     4501 accaagccgt agtagcagta gataatgttt ctaataaaac aaatgttgtg acaattgggg
     4561 ttgatcctaa cttgccacaa gttattgttc ctaaaacagg ttctacacat ttagtaacga
     4621 ttttagcggt cagcttacta ttacttgtgt tagcaacttt tggctacgca gttctgaagt
     4681 tcaattaaac aaaaaaacaa ggctgaaaat cagccttgtt tttctatttg aaaatgggat
     4741 tggcactaat aaatacgttg tctgaagaaa gtgatccgtc tgcagaaact tcataagcat
     4801 gggataaacc aacgatactt tgattgttgt aaagcaagtt tgcgttatga gcagcgctat
     4861 ctttccacat aaccatagca tttactgctc ctgtatctga aaggatctcg tcaacgtaaa
     4921 ggtagaaact attgatattt tctcctttag caccgtagca gcctaagctt tctaagagat
     4981 cttggaaagt accgccgcca ggtcggtaat gactgaagct tacttgtaat tcttttgcac
     5041 gaatatctgc agcttgttga acgatatcat tgaattgcat tggggcaacg ccattttgtt
     5101 gacggtagct atttaaccaa gcaagttctt tttgacgcac atagttgttt aaatcactta
     5161 gtgagatatt tttgtatttg aaagtgatcg ttcgattttg gtcaacatta ttgattacac
     5221 gttgtccatt tttatcaacg gtaacagatg aatcgtcatt tctacacaag aagtagtttt
     5281 gatcggtcat atttttccaa gaagtagaga aactatctcc ctcgtaaacc gtttgtgttt
     5341 cagtagataa aacagcgttt gtgtcaacgt ttatatggtt cacagtgacg ttgtattgat
     5401 gagtataggc aaaattgatt ccttgattac ttgtaacgtt gtttaaagtc acagtgttgc
     5461 tagagctttc tctaggatca agaacaaaat tagtgaacat aggtgcttca attgtgatgc
     5521 tttgtccata aacgacggga taactcttac tgtaattata gagttcttgg atatttggat
     5581 cagtatccga gactttattt ggacgagctt tcacttcaac tagaaagact tcatcatagt
     5641 aaaaagtaaa tgtggtattt gtgttaatcg tttgtgtttg tgtttcattc ccatttactt
     5701 gatactgtgg tgcttcaaag ttagtcaagt cggttctagc ttttgcagtg taacttgtac
     5761 cagcttttac tgagacatgt tcttctttta gcagcgttcc agtttgtcga ttcatatgac
     5821 ggacagttac cgcatattct gaaggtttag gtttgtgttg taacccttta aatgcagaat
     5881 caagactatt tttagcatta tttacttggg tttgagaagc tttagaatcg tctagtactt
     5941 ttttagcatt ggataaagca gtctgaaaat tattccaaga tccttctgta aaatctcctt
     6001 tagcagttga ttttacttgg ttgtacaagg cttgtaattc cgttttgttc acagtaggag
     6061 ctggtttttt ctttaatccc ttgtaagcag aatctaatgt gtttttggca ctatccactt
     6121 gtttttgagt cgcttttgag tcatttaaca cttttttagc attgtttaaa gcggtttgga
     6181 atgcgttcca ggtattgtcg gtatagtcac cttttttagt tcccttaacg ctcgtataaa
     6241 gagcttctaa agctgattta ttcactttgg gtttagaggt gtcagcacca taccaaccaa
     6301 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     6361 tgtagtgatg cgaaccagag gtaacacctg gaaggaaaag gcggtagatc ggtttattat
     6421 ttttttcggc agaacgccag gcaggccctt cataagtcca accgtaattt ttggtcaacc
     6481 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aaggccttta ttgtataaac
     6541 gatagacaag gtggccaccg ctagtcggtg cttcccaagc ggggccttca taattacccc
     6601 acttaatttt cactaggtga tcacgttctt ttgtgctaga ggtgtaaaaa tgttcatggt
     6661 tatttggatt ataaaggcga tacatgacgt tagttgaagc agcgtcagca gatccagcaa
     6721 gagcaactgt ggcagcaaat gttccaatag ctataacagt ttttgggttt aataatttca
     6781 tgttttcaac tccctattaa atgattaaat attttgttta cattcataat ataccacggt
     6841 ataccgaggt agtcaacggt ataccgtgga aattttgaaa taaaaaagcc gccctatgaa
     6901 gagtgtaagg atggctgttt atatgctatg aagaaatgga ttcggcgtat ttggttagtt
     6961 tggcagcatt atcaaatcgc attttttcaa tcgcactttt tttctttatt aaatccgcaa
     7021 ttgttgattc tgcgactgaa gaatgtttgc taatttcata tgctgtcttg ccagtttcaa
     7081 atagtaacca ttcgattttc tctttatcag cgtacattgc aatgctccct cttgattttt
     7141 tctttattat agctcttttt tgtaataaat cacaatatca agaggaaaag tttctataga
     7201 aaggtgggaa attatgagta tccgttcccc gtgtatcaaa atgaggtttg ttgctacttt
     7261 cgttatttaa tatactaaaa taaaacaacg aaagaaggaa tagaatgaaa aagttaataa
     7321 tggtatttac ttcgttgatt gccatgattg ttattttagg tggatgtggt agtgaaaaaa
     7381 ataagcaaga tagtcaagat aatggcgtgt taaaaccagg aacagtgtgg aaagaagaag
     7441 taggcggact tgttcataat ttaaaaatta tagatgatac aacatgggaa tattccgaaa
     7501 gtgtttggca tcctgaacaa gtacaaataa cagttgagaa gctaaaagac tataaaggat
     7561 tagaaagata taaggtaatc gattcaggtg gagtagattt attcataggt aaaacagcct
     7621 taatagttcc ctataacaaa gacggtttag aaacgtttaa atttattcca acaaacgaaa
     7681 aagaacatcg agataaaagt gaaatattaa aagaatacag tactaaatct ggatacaagt
     7741 tacaaaagac tagcgagtga ttgctagtct ttttatttat cctgaaggag gaatcgcagt
     7801 ggcaaaagct aaaacgccat ttgaaaaagc cgcgtctaaa atcttagaac g
//
LOCUS       DACCWK010000110         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCWK010000110 DACCWK010000000
VERSION     DACCWK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.114"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(138..428)
                     /locus_tag="IVW65_002318"
     CDS             complement(138..428)
                     /locus_tag="IVW65_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901172.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IVW65_002319"
     CDS             complement(546..806)
                     /locus_tag="IVW65_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901173.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IVW65_002320"
     CDS             complement(943..1356)
                     /locus_tag="IVW65_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7901174.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IVW65_002321"
     CDS             complement(1812..2297)
                     /locus_tag="IVW65_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901175.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IVW65_002322"
     CDS             complement(2436..2651)
                     /locus_tag="IVW65_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7901176.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IVW65_002323"
     CDS             2875..3675
                     /locus_tag="IVW65_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7901177.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IVW65_002324"
     CDS             3694..5562
                     /locus_tag="IVW65_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7901178.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IVW65_002325"
     CDS             5555..6046
                     /locus_tag="IVW65_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7901179.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IVW65_002326"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IVW65_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7901180.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IVW65_002327"
     CDS             6606..>7600
                     /locus_tag="IVW65_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7901181.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1443 g         2236 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACCWK010000111         7539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCWK010000111 DACCWK010000000
VERSION     DACCWK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.115"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(107..1405)
                     /gene="eno"
                     /locus_tag="IVW65_002328"
     CDS             complement(107..1405)
                     /gene="eno"
                     /locus_tag="IVW65_002328"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7901182.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1521..2276)
                     /locus_tag="IVW65_002329"
     CDS             complement(1521..2276)
                     /locus_tag="IVW65_002329"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7901183.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2380..3570)
                     /locus_tag="IVW65_002330"
     CDS             complement(2380..3570)
                     /locus_tag="IVW65_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7901184.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3680..4681)
                     /gene="gap"
                     /locus_tag="IVW65_002331"
     CDS             complement(3680..4681)
                     /gene="gap"
                     /locus_tag="IVW65_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7901185.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4722..5759)
                     /locus_tag="IVW65_002332"
     CDS             complement(4722..5759)
                     /locus_tag="IVW65_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7901186.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="IVW65_002333"
     CDS             complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="IVW65_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7901187.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7356..7427
                     /locus_tag="IVW65_002334"
     tRNA            7356..7427
                     /locus_tag="IVW65_002334"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7389..7391,aa:Arg,seq:ccg)
BASE COUNT         1955 a         1587 c         1389 g         2608 t
ORIGIN      
        1 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
       61 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
      121 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
      181 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
      241 ggccagcatt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
      301 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
      361 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
      421 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
      481 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
      541 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
      601 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
      661 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
      721 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
      781 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
      841 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
      901 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
      961 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     1021 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     1081 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     1141 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     1201 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     1261 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     1321 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     1381 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     1441 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     1501 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaaaaa tgaatctgct
     1561 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     1621 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     1681 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     1741 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     1801 acttgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     1861 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     1921 tttgcaaaga tagcttttgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     1981 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     2041 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     2101 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     2161 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     2221 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     2281 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     2341 ttgatcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     2401 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccaccac ctgttgagat
     2461 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcagctgagt ctccgccacc
     2521 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     2581 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     2641 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     2701 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     2761 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     2821 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     2881 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     2941 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     3001 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     3061 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     3121 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     3181 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     3241 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     3301 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     3361 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     3421 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     3481 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     3541 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     3601 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     3661 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     3721 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     3781 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     3841 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     3901 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     3961 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcaggaa
     4021 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     4081 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     4141 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     4201 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     4261 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     4321 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     4381 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     4441 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     4501 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     4561 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     4621 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     4681 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     4741 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     4801 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     4861 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     4921 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     4981 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     5041 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     5101 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcacg gtggttaccg
     5161 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     5221 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     5281 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     5341 ggaagtagtc ggtctagggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     5401 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     5461 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     5521 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     5581 tttttcaaaa gatctgtttc tgttcgcaat actcgttctg tgatccccat actttccgat
     5641 aaacttcgac gtccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     5701 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     5761 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     5821 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     5881 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     5941 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     6001 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     6061 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     6121 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     6181 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     6241 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     6301 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     6361 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     6421 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     6481 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     6541 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     6601 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     6661 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     6721 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     6781 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     6841 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     6901 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     6961 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     7021 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     7081 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     7141 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     7201 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     7261 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     7321 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     7381 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     7441 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     7501 aattggtgga cagttttttg ttgtacctgt atagatatt
//
LOCUS       DACCWK010000112         7339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCWK010000112 DACCWK010000000
VERSION     DACCWK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.116"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..936
                     /locus_tag="IVW65_002335"
     CDS             <1..936
                     /locus_tag="IVW65_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7901188.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IVW65_002336"
     CDS             939..3122
                     /locus_tag="IVW65_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7901189.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IVW65_002337"
     CDS             3119..4120
                     /locus_tag="IVW65_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7901190.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4117..5049
                     /locus_tag="IVW65_002338"
     CDS             4117..5049
                     /locus_tag="IVW65_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7901191.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            5294..5410
                     /locus_tag="IVW65_002339"
     CDS             5294..5410
                     /locus_tag="IVW65_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7901192.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..>7339
                     /gene="tet(M)"
                     /locus_tag="IVW65_002340"
     CDS             5426..>7339
                     /gene="tet(M)"
                     /locus_tag="IVW65_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7901193.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKI"
BASE COUNT         2368 a         1341 c         1555 g         2075 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaatga gaaaggaaga tttaatgatg
     4141 aaatttagaa aaaatcagaa taaagaaaaa cagataccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcataa aaaggttgtg attgccttgt gggtactttt agggcttagt
     4261 ttcagctttg cgatattcaa gcactttaca gctatagata ctcatactat tcacgaaaca
     4321 actatcatag aaaaggaata cgttgatact catcatgtag aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggagcaatcc gataagtcca ttgataatcg aatggaaagt
     4441 ctaaaaggct atctgacaga tgaacttcaa gctctcaatg ttgatacagt acgcaaagat
     4501 attcctgtat cgtcttctgt aagaggattt cagatatgga cggtagagcc aactggcgac
     4561 aatgagttta atgtaaccta cagtgtagac cagctcatta cagagggaga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagatg gttctggaaa tatggtactg
     4681 gttaagaatc cgaccattac caacatacct aagaaatcaa gttataaacc aaaagccatt
     4741 gaaagtgagg ggacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 gtaaaataac aaatattggt acatgattac agatactttg taatcatgta ctctttttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtgactatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaata
//
LOCUS       DACCWK010000113         7289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCWK010000113 DACCWK010000000
VERSION     DACCWK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.117"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..707
                     /locus_tag="IVW65_002341"
     CDS             <1..707
                     /locus_tag="IVW65_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7901194.1"
                     /translation="IDADAETLTKRFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLG
                     IMTAAVMKIMPKMVAMFMEGLMPIAEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMS
                     TSLLLVPISLLLALILPGNKVLPFGDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVT
                     TLYMATWLAPIITDVFTLASFDVGTAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLG
                     VVLVGASIYMNKVKKA"
     gene            741..1025
                     /locus_tag="IVW65_002342"
     CDS             741..1025
                     /locus_tag="IVW65_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7901195.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            1027..1542
                     /locus_tag="IVW65_002343"
     CDS             1027..1542
                     /locus_tag="IVW65_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP7901196.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            1558..2220
                     /gene="rpe"
                     /locus_tag="IVW65_002344"
     CDS             1558..2220
                     /gene="rpe"
                     /locus_tag="IVW65_002344"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7901197.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            2227..2799
                     /locus_tag="IVW65_002345"
     CDS             2227..2799
                     /locus_tag="IVW65_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7901198.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(2986..4506)
                     /locus_tag="IVW65_002346"
     CDS             complement(2986..4506)
                     /locus_tag="IVW65_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7901199.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(4749..4928)
                     /locus_tag="IVW65_002347"
     CDS             complement(4749..4928)
                     /locus_tag="IVW65_002347"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7901200.1"
                     /translation="MLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQAL
                     FLFCGTKKDRFKALY"
     gene            complement(4918..5100)
                     /locus_tag="IVW65_002348"
     CDS             complement(4918..5100)
                     /locus_tag="IVW65_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901201.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            5341..5559
                     /locus_tag="IVW65_002349"
                     /pseudo
     CDS             5341..5559
                     /locus_tag="IVW65_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            6054..6653
                     /locus_tag="IVW65_002350"
     CDS             6054..6653
                     /locus_tag="IVW65_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7901202.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            6717..>7289
                     /locus_tag="IVW65_002351"
     CDS             6717..>7289
                     /locus_tag="IVW65_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901203.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         2398 a         1211 c         1367 g         2313 t
ORIGIN      
        1 aaatagatgc agatgcagaa acattaacaa aacgtttcgg tgttttaggt gacacagttg
       61 ttattggcct aattatcggt attgtagtgg gtattatggc aggctatgac gtagcaggaa
      121 ttggaaactt aggaattatg actgcggcag tgatgaagat tatgcctaaa atggtagcaa
      181 tgtttatgga aggtttaatg ccaattgcag aagcggcgaa agaatttacg aataaaaagt
      241 taaatggtcg ccaagtaaac attggaatgg atgcagcgtt aactgtgggt catccaacta
      301 ttatgtcaac ttctctattg cttgtaccaa tctcattatt gctagcacta attcttccag
      361 gaaataaagt attacctttt ggagatttag cattctttgc tttcgccatc tgtttaatga
      421 ttccttattt taaaggaaat attgtgagat cattagtagg atgttcgttg tatgttgtta
      481 caacattata tatggcaaca tggttggcac caattatcac tgatgtgttt acattagcaa
      541 gtttcgatgt ggggacagca ggattagtaa ctatgttgtt atgtggatta tggccagtag
      601 ggttgtttgt tttattagca tcttactttg gtgtaattgg aataggtgga ttaggagttg
      661 ttttagtagg tgcttctatc tacatgaaca aagtgaagaa agcttaatca taaaattaaa
      721 ataagaaaga agagatcaat atgaaaaaat tattagttat ttgtggagca ggtcatgcta
      781 cttctacaat tgcagtagca aaattatcaa aatggttgga agaagaagga ttaacagatc
      841 aagtgaaaat ttatcaatct aaaattgctg atgagctaaa taaaattgat gaatatgatg
      901 ctgtagtaag tacaacaatt gtccctgatg taattaaagc taaagtaacg aatggcttgg
      961 gcttattaac aggtattggt acagatgcca tttatgcaga tgtaaaagaa agattaggtg
     1021 tttaatatgc gttttcatca tgacactttg actattgaaa cagtttcagg gcgtccttcg
     1081 tatcatgata ttcgaaaaga acttttagat acagttgata cagcaggtat tgagaatgga
     1141 atgttagtaa tttcgagccc gcatacaact tgctcactct tttttgaaga aacgatgcat
     1201 gacacaaact tttttggtga tgattattta catgtagaca tcaacaatgt aatggaaacg
     1261 attattccaa aaatgacgag tgaaaatcaa tacaacagtc cggggccaaa acacattgat
     1321 tttggaacaa gattatctga tcccaattac cctgcagaaa aatgggtcat gctaaatacc
     1381 gatgcacatt tacgttcatc tatttatgga accaactcaa tgactttaat tgttcaagaa
     1441 ggtaaacttt tattaggaga aatgggccgt gtctattttg tcgattggga tcaactaaga
     1501 gagcgaacta gaacaatcaa tctcatgatt atgggagaat aaacggggga atagagaatg
     1561 acaattgctc catcattgtt aaatgcacct atcgcagaag ttaaagagtt atttgaaatc
     1621 ttaaaagaaa agaatgttga ttatttgcat attgatgtaa tggatggctg ttttgtacca
     1681 gatatggctt ttggaccaag tgttattaaa gatattcgtc cactaacaga cgttgtgttt
     1741 gatgttcatt tgatgattga tacaccagaa aaaattgtca caagatttgt agaagcagga
     1801 gcagatattg ttaccttcca cattgaagct accaaacacg caatgaaggt cattcagtta
     1861 gtcaaggaag caggagcaaa agtagggatt acttttaatc ctgcaacacc agtcagtttt
     1921 attgaaccag tattatcttt agtcgatcaa gtattagtaa tgacaatcaa tccaggaaca
     1981 gagaataagc attttattca agagacagta gttaaaatcg aacagttaga tgtaatacgc
     2041 aaacaaaatg attatacgta tgatattgaa gtagatggca agattgataa tcaaacgatt
     2101 aaagtttgtt ctaaagcagg ggcggatatt tttgtatcag gcggtttcat cttcaatggg
     2161 aaaaataccc cgcaagaaca aattgataaa ctaaatcaat cattagagga agtgaagtaa
     2221 aagatcatga ccaataaaga ccaaaataaa aactatttag aagcgattat cttagaatta
     2281 caaaataact caaaacgtat taattctgta gagttagaac aatttagtga gaaaattgtt
     2341 caagctaaac acatctttct agctggtgca ggacgctctg gaattgccat gcaagcattt
     2401 acgaatcgct taatgcattt aggtttttca gtcagccttg tcggtgaaat tagttcacca
     2461 cattcgcaac ctggagattt attaattatc tgttctggct caggtgaaac aggtagttta
     2521 aaaaatttag cggccaaagc aaatcaaagt ggtgtaagca ttgctttagt taccatgaaa
     2581 aaagactcgt cgattggtca attagcagat tctgttcttg tattaccagg tattaccaaa
     2641 gatgaaaatc aacgagaaga agatactttt tcacaaccta tgggttcaag ttttgaacaa
     2701 ttagcatttc tcacctttga tagtttagtg ttatctttaa tggataaaac aggtgaaagc
     2761 tcagaaacga tgtttgctcg acatgcagat tttgagtagt agattttaaa gataaaaaac
     2821 cattctctaa atgctaatta ttcagcaaat atgagaatgg tttttttatt tgattagaca
     2881 taacttaata attcagtagt tttgagcgta agcgtgtcat agaatggtac ctaaacacaa
     2941 atagcccgat ttgatacact caaatcaggt tatttgtggc ttatattatt tacagatttc
     3001 ttgtatctgc tgattccatg gcaaataagc gcgtagcgct tcaggtgtca tcgaagacag
     3061 attgggaatc tctgtaaaaa gatgatttaa atatttgcgg atgtttaacc cattcgcaac
     3121 agctgtctgg ataatactca atataattcc atttgatcta gcgcctttta agcttttaga
     3181 aaatagccag ttttttcgac cgatcactaa ctctttgatg gcacgctctg ctcggttatt
     3241 tgaaagttcc aagcgtccat caagcaacac aatctgaagt ccgtctttat gattgactgc
     3301 gtattgaacc gcctttccaa gctgcgattt tggcaaataa gcaaaactct cacaccaatg
     3361 aaaaaattca gtcatcacag gctgcaacgt ttccttcctc gctacaaacc tttcttctgc
     3421 gctaagctta ttaaaggtcc gttcgattcg aaacatcttg tcacagaagt ctaatcctat
     3481 tttggcatga ctttttgggg acggttgttt acccaatgct tcaaaaaact ttctgcgaac
     3541 atgcgcccaa caataaacat tgtttaccgg cagctttcca taggccgcgt aaccatcagt
     3601 atgcaagaaa ccgctaaatg attctaaaaa actttttggc tcatcaacgg aacgcgaatt
     3661 tgcgtgctcg tagagcacaa cttgtttttc ttcgctttta cctgaagcat ataaccagta
     3721 aaaggtcttt tctttatgag tattcaatac ccgatagctt gtctcatccg cgtgtaagac
     3781 ctcctgattg cttaattctt gatgaaggca attgtagatt ggctctaaaa ccatttcact
     3841 taccttgata tgccaattag tgattttttt acgatcaaga gggaaaccaa gtgcatgcca
     3901 ttctttttct tgtcgatatg ccggcacctt tttaacaaat ttttcccaaa tactctgtgt
     3961 cacaattgaa gaggaaccta gactattatt gatcaacggt ttaggaacag gcgctttttt
     4021 gatttttgtt tctcctaatg cttcacattc tctgcaatga taggcatgtt ggtagatgac
     4081 ttgtttttct atatgggcag gaacatacaa tggctcttct ctaagccttg tcgcaccgat
     4141 atcatgtaaa gtatgaccac agtcttcaca caaacaatcg gcacctttta attcataatg
     4201 aataggaact tcaggcagac cttctaatga aagtgctttg agtcccttgc gttttctgcg
     4261 tttaaaagaa gataccgtaa tgggttcctg gctttcttgt ccagtgtgct ctggttcatt
     4321 aaaagttgag tcgtctaata aatcgatttg cccatctacg ggtggtgttt ctttttttcg
     4381 tccaaacaag cgtcgattca tttcagcaat ttgttctcta agaagagtat tttcctgttg
     4441 ctggatttca agcaactgtg ttaattgctt gatttgtaca agaagttcag attccatttt
     4501 tgtcattttt acaccaccat tctagcgatt ttcttacatc tattatacca ttttatgaca
     4561 acgaaaaaca gacaaaatcc ttgatttaac aaggttttcg tctaatatat cgcacctttt
     4621 ttcgtttctt ttagcgtaca tttttcttcg attgaaaggc cagtcagtaa gacttctttt
     4681 ctggaacgcg gccattgaag gtacccactt tcaaatcttt tgtacattaa tacaaatcca
     4741 ttttgatcct agtaaagagc cttgaagcga tctttcttcg taccgcaaaa taagaaaagc
     4801 gcttgatcgt aaacatcaag gtcatattct tctataacga aagctgctag tccatcaatt
     4861 ccttttcgaa gatctgtttt tccacaaacg ataaaaatat gtttcaatgc agttgcatca
     4921 atgagcattg gcattcaact cttttaacaa ggtatacaaa agatatttat ccattccatt
     4981 ataaataaat atttccttat cttccgattt gattctgcaa gctagttttg gcttggtgtc
     5041 aatttgcgat ttttgagccg cacttgaaaa ttgaactggt acaatatctt ttttattcat
     5101 tcgtttttct ccttctatat ttgatagttt aattatagaa gaaagataat cggcgtacga
     5161 gatactatcc tatgacacgc ttacatttaa tttaatattt tgcctcaaat tagccacact
     5221 ttttatagaa ctaagaggaa aaatgaagtt ttcttcctat taatatagtt gtttgaaatc
     5281 ttaaatatag cgttttacga tgtaatggga agtatggtga ataaatcaac tatgttcgta
     5341 cgaagcattt actcaactaa tttaatcgaa tctttcaata aacaaatcaa gaaatacagc
     5401 caccgcaagg aacagttcca aaatgaagag tcgatggaac gtttcttagt ctcgtctttt
     5461 gatacttaca accaaaaatt cctaggtcgc agtcataaag gtttccaaca ggccgaaggc
     5521 gagctcgaac aaatgctgag tcaaccgatg gagaattaga aacaatctac agtagggaag
     5581 aaccattgac acaaaattat tgacgctccc aactaaaagt gtcaaaataa tgatgaaatc
     5641 atttagttct tataaattag aaaagagaaa ataaatattc catatctact ttattggatc
     5701 cgtaacatga ctaaattctg aatacttttt aaagcttatc atttggtaaa actgaatata
     5761 agttaattat atagactgaa aattttgaat tttcagaata gaataactta aaagtactga
     5821 caaaattttt taaaggggca atattaatgt tctgagataa tcaaatccta ccgaaaatat
     5881 caaaaatcca atgtgacaca atcagattgt gtctcattcg agggaatcac acaatcccac
     5941 gtggctctag gcagaattga atttttgctt ggggcttttt ccttatattg attttgaatg
     6001 agacttatta tatgttataa tgcaattaag tatcattgga gagggcgaag aaaatgaaga
     6061 cggtagaacc tattcgagac aaaaaaaaga tcgatgcgat gaaggcaatc ttggcttcgg
     6121 gaaaatatgg ccaacgaaat ttggttcttt tttcgattgg gatcaacacc gcctatcgca
     6181 tttctgattt gcgtcaattg aagctggccg acgtgctgga aatttcacgc ggtcgcgtgg
     6241 tggtcaaaga tcgattagcg atgaaggaac aaaaaacagc taaacataat tcggtctata
     6301 tttccaacaa attgcgaaaa acgattttgg agtacgttca acaggaattt cctgatcaac
     6361 tccttcagca agattttgac ggttatttgt tccctagtcg aaaagggacg aatgaaccgc
     6421 tcaaccgcca aagtctgtgg cgaatcattc atgatgctgg cacggcaatc ggattgaaag
     6481 acattggccc acattccatg cgaaaaacgt tcggttactt tctctataaa caggggacca
     6541 aaacagaaat cattcagtcc ttgttgaacc attcttctca gcgcgaaaca ttgcggtata
     6601 ttggtattac acaagaagac aaagatacag ctgtaaaaag cttagattta taatataaaa
     6661 gttaagttta aacatggata gttttaaatt agtagattga gtgttgggag gaaagaatga
     6721 gtacattata tattatagga aacggttttg atttaagtca tggtttaaaa actagataca
     6781 ctgatttttc aaaatgggtt gaaaaaagtc atagtgaaat attcgataaa atagacacaa
     6841 tgataaatga aaataatgag gtgagaagta cagctgatga taagataggt tggtttaatt
     6901 ttgaaagtag tttgcatgag ttaattcaaa gtccagtctt tgaagattat gtagaagagt
     6961 ctaaggatta tctcataggt tatggacatg aagattggtc agattctgcc catcatgatt
     7021 ttcaaaatat ggtttatgat tatgttgttt ctctagaaga attacaacaa cttttcaagg
     7081 aatggatacg caaagaaata cagccgacaa tagaacataa aattccaaaa taccaatttt
     7141 cgtccgatgt actattcttt acattcaatt atacagatgt tttggaaatt ctatacaagc
     7201 aaccgaaaga aaacatatat catattcatg gcagttatga cgatataata atcggccact
     7261 ccgagaattt tgacaacttt ataggagat
//
LOCUS       DACCWK010000114         7148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCWK010000114 DACCWK010000000
VERSION     DACCWK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.118"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(62..1453)
                     /locus_tag="IVW65_002352"
     CDS             complement(62..1453)
                     /locus_tag="IVW65_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAP7901204.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            1579..1989
                     /gene="tagD"
                     /locus_tag="IVW65_002353"
     CDS             1579..1989
                     /gene="tagD"
                     /locus_tag="IVW65_002353"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP7901205.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            2169..3458
                     /locus_tag="IVW65_002354"
                     /pseudo
     CDS             2169..3458
                     /locus_tag="IVW65_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3525..4952)
                     /locus_tag="IVW65_002355"
     CDS             complement(3525..4952)
                     /locus_tag="IVW65_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7901206.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(4949..6091)
                     /locus_tag="IVW65_002356"
     CDS             complement(4949..6091)
                     /locus_tag="IVW65_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAP7901207.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            complement(6095..7090)
                     /locus_tag="IVW65_002357"
     CDS             complement(6095..7090)
                     /locus_tag="IVW65_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7901208.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
BASE COUNT         2557 a         1194 c          974 g         2423 t
ORIGIN      
        1 aaaatttgtt gaagttggga taactagttt tcttttctac tttatatacc taaaaatagt
       61 ttcaattagt tactccttct tctttagata tagttttttg gatagaaccc agatatcccc
      121 aaaaaataaa tgaacctatc gtactaacca aaatgagttc tgtgataaat aatgccgaaa
      181 ctgctattgt aagaatcatt aaaatagcaa aaaagtgctt atcttttact agatttggtt
      241 gtgtgaaaca ataacgcaaa gttttaaagc attggataaa taagaaggtt aacaatgtca
      301 atagacctaa tcctcctgta gcagccataa ctaagaaaat aaaattatga gcagttaaag
      361 tatcttttcc cattaatgta tatggtagtt tatcatgtgc gtattcaata tagtatcctg
      421 gagaaccgcc ggatatcggc ttactcttga atatttcaat tgcacttttc caaagtccaa
      481 accttaaatt agaaacatcc ccgccatcag tgtcagggcg ttttaaatca actttattgc
      541 tgctagttgt tcctgatgaa tcagatatat ctttttttat ttctgtaata tgcggaacat
      601 gtgcccatgc ttgagcagtt atatctgtta cataatacat cgcaattata cttagcaatc
      661 ctactactaa tgcagttagc catcttttta aagtattagt tttaagaaaa cgactactat
      721 aacttgtaaa gaaactaaaa acaaaagttg ctattaataa acaaactaat gtggtgcgtg
      781 agcccgataa agcaataaac gagaattgaa taaaaatatt aattgcaaaa aatattttaa
      841 atagaagatt taaatgcttg taagtgacca aataatatat agcaataatg atgacaatta
      901 atgatataac cgcaccaaaa tttggatctg tatatacacc aaataaacgg ttgcctaacc
      961 accctaaacg aactgggtaa tacttatatt ttattggaat ttttatgcca tattgaatga
     1021 caaaccagta taatgatact aaattagcta aaaaccatac caatgaaagt atccaagtaa
     1081 atactttgaa aaaaaagtta ctactgtagt ctttgccaaa ctgataaatc ataaagaaaa
     1141 agattaattg ccacatgatt aacttgatat tttcgctata tccatatttt aaattcatta
     1201 aactgcttac tagcatcgcg ccaataaaag caattaatag ccaatcatat tttttaagat
     1261 ttttttctct aaatgcaata attgcatcta taatgatcaa gacaactccc gctattccag
     1321 caggccaaaa aagtaaagta tcatatctag taggtaatag taaatacggg cctattgctc
     1381 gtaacattag aataaaacca aaataaataa aataccaacc tttcacatat tttaaaaatt
     1441 tttcattcaa catttttcct ccatccacct atttctcata aacatttctt atccttttta
     1501 ggataaactg gttctttcaa ttctaacaaa aaaatgctaa actaacaatg tatattaatg
     1561 aatttcgaga ggtgaaagat gaaaagagta attacttacg gtacctttga tttgcttcac
     1621 tacgggcata tcaatttact gaaacgagca aaacaatatg gagattatct tattgttgcc
     1681 ctttctacag atgaattcaa ttggaatgcg aaacaaaaaa aatgttattt tagttatgaa
     1741 aaaagaaaac agttacttga agctattcgc tacgttgatt tagttattcc tgaagaaaat
     1801 tgggaacaaa aagtaaaaga tataaaagaa taccatgcag attatttcgt gatgggcgat
     1861 gattgggctg gacaatttga tgaacttgaa caactaacag atgcacaagt aatttattta
     1921 ccaagaacac ctgagatctc cacaactcaa ataaagaacg aacttaattc tttaaatagt
     1981 gataaataat attttttaaa caatgaaaga acaatatatc tggataagac gtaaaaaaat
     2041 ggctctataa tttctaggac tgatagaatt ttctgtcgat cctagatttt cttgtaaaaa
     2101 aaacagacat acaaaaattt cgataaaata gagtcgtcta aaacatcaat ctgaaaggaa
     2161 gattttgtat atccatttct cgttctatca aagagcactt cactattaaa gataaaaata
     2221 ttgaaattaa ttcttttcaa aaaacttttt ataaggacaa gaaagccatc gtaatcgaag
     2281 gtacattagt tcgcactttt agacattgtc tattttgtaa agaaactgcg aagcagctcg
     2341 tgaaaaacgg caagaagatt tcaatgatta tcttaaatcg cagtgggaat aaataaacct
     2401 atttgcgtct tagaaaacaa cattaccact accaatcatg cggaaaatat tttactgctc
     2461 atacgtatct tgtcgttcct tcatgtttta tttcgaaaca agttcattac acgatattag
     2521 aagagttgac cgagcgtcaa gcgttaaaga cacttacgaa acgttgtgat ctttcagtaa
     2581 cgacatttca acggacatta tctgctctat cggcaatgct gcgacagatg ctagactatt
     2641 tacctcattg tttactattc gacgagtttc gctctctttc aatttcccat ggaaagttct
     2701 ccttttcttg tatgaataga gagacaggga agctctttga cattttgtcc agccggagaa
     2761 aaaaagattt gattgcttat ttcatgcgtt ttacaagaaa agctcatatg ggggttcgat
     2821 acattgtcac aaatatgaat gccccttatt tttcattagt caaagagtcc tttccaaacg
     2881 cccaaatcat cattgattgc tttcacattg gacaacactt gaacaagttt ttcgatcgtg
     2941 ttcgaaaacg tgtgatgaag cagttgaatc aaaaagacac ttcacaagct aagtactatc
     3001 gacaattgaa acctttgtat aaattacttc taaaatcaga tgacgagttg gattacacaa
     3061 cattcaagaa atggcagaat tttcaatggc gctatttaac agaaagtgaa gtagttgatc
     3121 gtctaccctc aatctcttat gaacttaaaa ttgctaagca gtttatcaaa agctgcttag
     3181 caattatcac aagaaacagc tagctacttc gagaaacgac caaagaagtt ccctcgaaac
     3241 ttcactatct caaaaagcct ttttcatatc tgaaaaaggc ttttaactag cattttcgaa
     3301 aaagtattct aataacaaaa ttagagaatc ttcgcacaca tatcaaaaca ttgaaacgat
     3361 ttgcttatgg gtttcgcagt tttaccaata tgcgcttgtg gatttttcta atcaacaggt
     3421 taatcagcta tgcattaaaa gctagtaact cttactaact ttgttgttca taaatgacct
     3481 tatcagcccg atttgacaaa gaactacttt tttacaacat tattttattt tcgagtaatc
     3541 tttcttattt catttcgtat atctggaaaa agaaaataaa caaacggaag ataaataatg
     3601 aaaatcatta ttgatttaat tgccatatct aaaaatgaag aagagaataa ttgtggaaga
     3661 aagtaatagg cccaagtagt caaagtaaga ttgactataa aaaataagat aacgttcttc
     3721 ccaaagaaat aggatatttt tatttctttt cgtacaaaaa atactctaaa gaaaaagact
     3781 aataattcta ctaccacgag tacgatcgta gccgctacag cgccataagt aggcacaaaa
     3841 gctacatttc caataatact tacaatcgca ccaatgatca ttggataagc atactcctta
     3901 tctttttcta tagcaattgc aaattggtta gcaaaaactc caccaattgg actaacaact
     3961 ataattagac aagaaataag catgtttatt gtcatcggtt tgaaattctc cccaaaaaac
     4021 catggaacaa attcttttgt attgataaac accacactaa aaagaattaa tgcaatgata
     4081 atcgtgtaat caaaagattt ttttgtgtaa actaaaatat tttctgattt cccctctgcc
     4141 tgtgctttag atattttagg caacataaca atagtaattg atgttaagac tgccaatata
     4201 atacgagcaa ttttttgaga ctgatcataa taagataatt ccgttggatc cacaaaatac
     4261 ccaactatcg ttttgtctaa agttgtgtac acttgcataa acaactgagg agtcaaaata
     4321 ataaaagctg gtttccacaa ctgtttaaaa acattagagt taaagattgg tttacctact
     4381 tttccgtgga tacttaacca gaaaactaaa cttcctaaaa agttagtaat tgaagtaatg
     4441 aacacgtaaa tccacaaatc atctttagtt gaaacaaaaa tgattattaa caataccgat
     4501 gataatttga taactgtatt tctaataacc actttcgaaa attcttcaat tccttgaaaa
     4561 aaccaagaaa tatctaatgt tagccctaca ataaatatca attgaattaa gtataaattt
     4621 ttatacttta catcaaaaaa ataaatatag gctatataga gaaaggaaac actcaagcct
     4681 attaaaaatt gaataaagta aatattccag aattgtaaag atagtgcttt tttatctttc
     4741 cttgatttag cgatatttcg aatacgaaaa ggattcattc ccacagcaat gacagtcatt
     4801 aaaaactgtg atattgagaa aacataccca taaatcccta aaccagttga accaattcta
     4861 cgactaagta taggtatagt aagtaagggt aaaacaatca aaaagacttg atatatcgca
     4921 ttataaaata tatcttttac tgtttttttc actttatatc cctctctatc aactcaagaa
     4981 ccttttctgt acttgctcct gtttcataat ttccaatata atcaagtaac tcttgagaac
     5041 ttttcaaaag cttttcttgt tggtttttaa aaacatcaac aaggccataa ctatcttgta
     5101 cgcttataat tggtaactca cttaactgta tataaaaacc tctttctaag ctatattctt
     5161 tttcatcata gttaaaaaga ataattggtt tttttgttag cgcaaaatcg aaaaaaatag
     5221 atgaataatc tgtgattaaa acatctatct cttcaaaaag catttcaaga ggaatcccat
     5281 cattaattaa agtaatttga tgttttttca tatattctat acttttagaa tcagttgcta
     5341 atttactatg aacattagga tgaaatttag tcataaaatt taattcttgt tgttgctttt
     5401 taaaaaattc acgtaagtta tccacaccta accattctag ttgcgtaatt ccgttgtcct
     5461 caatattatt tcgaaaagtt ggagcaaata agacattgat ttgtttcttt ttcaagtcaa
     5521 acacttgctc tttactagag gctaattcat cgtttctagg agttcctact tctaatattt
     5581 tagaaggaac gatattgaat gctgaaacat acttcttaga actatagcga ttaccagata
     5641 ataaatatgt aattgactca ctatcttttt tcgcatagct taggtaacgt ttaggtaact
     5701 tatcttttgc atcaaattca atttttttta aaggcgttcc gtgccatgtt tgaaaataaa
     5761 gttgtttttt cctttttgca tacttaatcg ttttccgact atcgtctatc caatacttag
     5821 ctgtatataa ataaaaggca gctaaaaaag aatcggagtc aattctttta atataatctg
     5881 gtaaatcagt tataatcgct tcttttttga ttaaccaaat aattctgtaa ttcaattttt
     5941 tttcatgaat tttctctgaa ataactcttg gatttccaga atagttacgc ccgccaaaac
     6001 ttgaaaatac aattaaattt ttttgaatag gtaatatttt aataaaaacc gggaaaatat
     6061 tccttgaaaa aaattttttt atattcttca tctcttacct cacagattct atcttaatca
     6121 agaatttact tgctatctta ctacttgaca tatagtaagg caagttttta tttattaata
     6181 aaaactttac taattttttt aattttggtg aaaagtcggg atcaattttc acttcttttt
     6241 caaagcgttg taaaatattt atagaacgta aataatcttc tttattggta tagttatttt
     6301 taatcattgt catcatcatc cttattgctt catagacata gcgagttcta acattaattg
     6361 ataattccgg ataaaactta tctatgaata ttagtatatc ttcatggact tcaaaagaat
     6421 caaaaattct aggattgaag cttcctctca gtatactttt agggctttgg taatagtgat
     6481 atttagcaga tgaattgaca gcaacttttt cggaatgatc gatcagcaaa taagtagtag
     6541 ccttatcttc cattaattta cccagtggga atcttatatt atcaaataaa tttctattga
     6601 ataatttatt atttgcagaa tagccaattt tattatttat aaataagttt ttcattgctt
     6661 gttctctatc aaatatttcc cattttctct cagcaattgt atgttctttt agttgttcta
     6721 aacttgaaat cttttttatt tttagcaccg agatatctgc tccataagtt tgcgtgccaa
     6781 aatatagtaa ctcaagatag tctggttcta tccaatcatc gctgtcaata aagcacacaa
     6841 aatctcctgt tgcagaatca agaccagaat tcctagccgc acttaaacca ccattcactt
     6901 gatgtatcac ttttaatcgc atatctttct ttttatattc ttctgcaatt tccccacttc
     6961 gatccgggga accatcattg actactatta tttcaatatt tgaatgtgtc tggttggaaa
     7021 tagattctaa gcagttttct aaatagtctt ctacgttata tgcgggtact ataacactaa
     7081 ttttatgcac tctcttcact tcctaaaaat catctttttt atttttacaa atgtatcatt
     7141 accagtaa
//
LOCUS       DACCWK010000115         7041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCWK010000115 DACCWK010000000
VERSION     DACCWK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.119"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..1405)
                     /gene="gor"
                     /locus_tag="IVW65_002358"
     CDS             complement(59..1405)
                     /gene="gor"
                     /locus_tag="IVW65_002358"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7901209.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1539..2780)
                     /locus_tag="IVW65_002359"
     CDS             complement(1539..2780)
                     /locus_tag="IVW65_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7901210.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(2798..3721)
                     /locus_tag="IVW65_002360"
     CDS             complement(2798..3721)
                     /locus_tag="IVW65_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7901211.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(3942..6434)
                     /locus_tag="IVW65_002361"
     CDS             complement(3942..6434)
                     /locus_tag="IVW65_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7901212.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6441..6908)
                     /locus_tag="IVW65_002362"
     CDS             complement(6441..6908)
                     /locus_tag="IVW65_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7901213.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         1916 a         1548 c         1143 g         2434 t
ORIGIN      
        1 attttctggt tttacatggt ttgttcaata aaaagctgtt ggaactgtta atattaaatt
       61 atcgcatcgt tacgaattct tccgctcctg ttggatggat ggctactgta ttgtcaaagt
      121 cttctttagt tgcacccatc ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac
      181 caacaccaat agcatgtaag ccgacgattc tttcttcttt tccgacacag atcaatttca
      241 tttcgcattt ttggcgataa tcatttaatg caaagtacat tggggtaaag cgtgaacgat
      301 aaactttcaa ttcattttcg ccatattttt ccaatgcttc ttcttctgtc aaaccaattg
      361 ttgcaattgg tggatgcgta aagacgactg tcggaacaag tttatagtca agataagaat
      421 ctttctttcc attaaataaa cgttctgata aacgtctacc agctgcgatt gcaacaggag
      481 tcagatctaa tttaccgatc acatcaccaa cagcaaaaat atgttcatcc gttgtatttt
      541 ggtatttatc tactttgata aacccttttt tgtccaattc cacatttgta ttttccagac
      601 ctaatttttc ggtattcggt acacgtccac cggcaaaaag tacacaatca ccagttaagg
      661 tttcaccatt ttcaaatagc accgtatatt cgtcattgtt tttagtaatt tctttagcaa
      721 tatgatgtgc gtaagttttt attccagctt cttggtacat ttggatcaaa ttgtccgaaa
      781 gcattttatc aaacgttcta agtggtcgtt cttttcgata tgcccacatg acttcactac
      841 ctaaaccacg aaatacacct gaaagttcag ccgcaatgta tccagcacct aaaacgattg
      901 ctctcttagg taactctgtt aattcaaaaa atccatttga atcaattgcg tattctccac
      961 caggaatggt cattttactt gcacggcctc cagtagcgat caagatttta ggagcacgat
     1021 aggtttcacc gtttacttcg atcgtttgac tatcgataaa agtagcgtaa ccttttatag
     1081 cctcgacatg attgctatct aatcctcttt gataagcacc gtgtagaaaa tcaatatatt
     1141 tttctcgtct ttctactaga ccggcgaaat caacattttt taaatctgct tggataccgt
     1201 aacttgaggc atctcgttca attgtttcca aaatcgtact tgcttgccac ataacttttt
     1261 taggtacaca accgacgttg acacatgttc cgcccaattc attcgcttca atcagcaaaa
     1321 cttttgcgcc atgcatccct gctctgtttg ctgaagcaat accgccagag ccgccaccaa
     1381 ttacgatgta atcatatatt tgcattttgt tcctccttat tgaagagatc aatcaaaacc
     1441 tttatttaac gatccttttt ctacagaaaa caagtgtaac acttttcttg aattttgtat
     1501 gcgaagatgc ttaaaacagg gatatatcag ctttttcctt atttacgttt tcggatcata
     1561 tctccaggtt tcaccggctg tgcgactggc atagttaaaa tcatcattgg atttggtgct
     1621 cggtcaatgg attcgccttc ttcattatgc ataacttcaa cggtttgact gaaatgactg
     1681 aatcctggac cataaaattc gatttcatct cctacagaaa acaagtttct ctgacgtatc
     1741 gtagcaatct gtgttgcctc atcataagat aatacttcac cgacaaattt atatactggg
     1801 attttacgac gttcaccgaa taattgttcg ttttcagttg gtgtattgta gtaaaaacct
     1861 gtagctaatt ctcgttgtgc tactttccat aactcgtcga tccattcttg cttgcagaca
     1921 tagttttcag gatcttccat ataactgtct acagccgctt tatagacatt tgacaccgta
     1981 gatacataat ggatcgattt cattcttcct tcgatcttga agctgtctac gccgttttca
     2041 atcaattctg gaatatgttc gatcattgac atatccactg cactcatcga gaattcttct
     2101 tccacttcgc ctgtatcagt taaactgttt cgttcttgac caaaaggcat atcgaacaat
     2161 ccatacttcc agcgacaaga ttgagaacat ccgccacggt ttgcatcccg catcgacata
     2221 tggttggaca atgtgcatcg accagaataa gagatacaca tggcaccatg gataaatgct
     2281 tcgatttcgg tatccgtatt tttacgaatc tctgccactt cctccattga aacttcacga
     2341 gctaaaacga cacgttccaa cccttcttct ttccagaatt ccaatgtctc aaaattagtt
     2401 gctgaagctt gagtggataa atggatcggc agacctgggg cttctgtcgc acaaatttcg
     2461 atcaaagctg gatcagaaac aatcactgca gaaataccga catcacgcaa actgcggaaa
     2521 aattcccctg ctccatcttc atttccttca tgggtgacca tattagcagc aacgtatacc
     2581 ttcgcatcat gttcttttgc aaaagcaact ccttcagcca tttcttcata agtaaaatta
     2641 cctgcacggc ttcttaaacc atatgcattc ccgccaatat atactgcatc tgctccataa
     2701 tggatcgcgg tttttaactt ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc
     2761 aattttcttt ttactgtcat ttctttcatc atactcctta tttgatagca gaaggatcaa
     2821 tataaaagaa tcctgtatct aattcacggt tttcaggatg caatttactg atttcgctag
     2881 tcattttttc cgctttttct tgatcccagc taccttctaa aatttgtttt tttgcttcgg
     2941 cgaatagtcg agtgatgttt acgaattttt cgcctggagt aaataatccg tccaatttcc
     3001 atgtacagta atgatgctga taaagttcat tcaattcatt cgagagattc agatcattgc
     3061 tggcaaaaat atgtgtgcca tgactatctt cataaataga ataatgtgtc tcttcttttt
     3121 taggttccga gataaaaaga cctctttcac gatctttttg ttcgtcttgt ttcgtatagt
     3181 tatagtaatt ctgtagcagt ggacgtttgg attgatggat gcatgtagca ccatacacaa
     3241 gggtttctac aggaatatcg agtttttctt ccatcgcctt catctcttca aaaggaactt
     3301 cacgagcaag tactgctcca actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc
     3361 tcgttactaa tgtttcagca tcataaatat acggtatacg caattcagga ttttttttca
     3421 tcgtataaat aatacctgta tcaccaactg tgatcacatc tacgtttatt tcttttaaaa
     3481 aagccaaata ttcaggtaca tttttcattt tttcaggatg aatgatccca tttacagcaa
     3541 tattcgcttt tttaccatac ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg
     3601 taaatgaagc aggcagacgc aatccaaatt gctcttcacc gaaatatagt gtatctgcac
     3661 ctgcagcaag caactgttct gcttgttcaa tagtttctac tgtggttatg agttcgatca
     3721 taattttctc ctcctacaac ttcataatag tagcatagct ctaaaaactt tccaatactt
     3781 cttctattta aagtcggatt tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat
     3841 atattctgtc caaatataaa aagagattgt gacaaaaatc ttgtcacaac ctcttaatca
     3901 agtcttacta ttataaaaat tcagctacac aggccaagta actataggga acctactgtt
     3961 tcctttttag gtccgcgaac attcaatgtg atcttacctt ttgaagcacc tactgttacg
     4021 tggtcaccta aacgaatttc tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct
     4081 ttttgaagtg cgcgacggat tggacgtgca ccatattcag gatcaaatcc agctttcccg
     4141 atcacatcga ttgcagatgg tgtgattttc acttgaatat cttgttcttt caatcgtttg
     4201 ataatcgctt tgctcatgat ttggacgatt gtatggattt catctttact caatgaatgg
     4261 aagacgacag tctcatcgat tcgattcaag aattcaggtc ggaaagcttt ctttaactct
     4321 tctaagatac gtttttgcat ggctttgtga tctttagtaa catcttggac attgaatccg
     4381 acattttttt cttctcgaat ttgtgtcgct ccgatatttg aagtcataat catgattgta
     4441 tttctaaagt ccacttttct acctttagaa tctgttaaat gaccatcatc caatacttgc
     4501 agaagtaaat tgaaaacgtc tggatgtgct ttctccactt catctaagag aatcactgaa
     4561 tatggttttt gacgaatttt ttctgtcaac tgtccacctt catcataccc tacgtatcct
     4621 ggaggagacc cgattaatcg gcttgtactg tatttttcca taaattctga catatcaaca
     4681 cggatcaggg catcttcact tccaaacatg acttctgaaa gtgctttggc caattctgtt
     4741 tttccgacac cagttggtcc aaggaacatg aatgaaccaa ttggacgatc aggatctttc
     4801 aatccgcttc gtgcacggcg gatcgcacgt gaaactgctt tgacagcctc atcttgacca
     4861 acgacacgtt catgcagaag tccttccagt tctaacaagc gttcgctttc ttttttctcc
     4921 aactgctgta gagggacacc agtccactcc gatacaactg tagcaacatc ttcttctgtt
     4981 acacgatcag aataaccttg ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt
     5041 ttacgtagtg ctttttcttg tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg
     5101 atcgctgctt ccttttcttg tactagttca tcgatttcta gtttgatcac agttgatttt
     5161 gttaagtgat cggtttgatc caaacggact ttcgctgctg attcatcgat caaatcaatt
     5221 gctttatcgg gtagttgacg agaagtgata taacgaacag aaagattgac tgctgctcgt
     5281 acagcttcat ctgtgatctc cactccatgg tgctcttcat atcttgtacg taatccttga
     5341 agaatcactt ctgcttcttc aggagttggc tcatctactt gaatgcgagc aaaacgtcgc
     5401 tctaatgcag aatctttttc gatatatttt tgatattcat ctagggtcgt tgctccgatc
     5461 gtttgtaatt ctcctcgagc taacgccggt ttcaagatat tcgaagcatc aattgctcct
     5521 tctgctccac ctgcaccaat taaggtatgc aattcatcaa tgaataagat tacttgacca
     5581 tcattataga tttcgtcgat gacctttttc attcgatctt caaattcacc gcgatatttc
     5641 gtaccagcaa ccaaagatcc catatcaagc atcatcaggc gcttttcttg catgtcttct
     5701 ggcacttcac ctaaaatgat tttttgagct aacccttcag caatggctgt tttgcctaca
     5761 ccaggttcac ccaccagtac agggttattc tttgtccgtc ggctaagaat ctggataagc
     5821 cgtttcactt ctctcgaacg accaacaact gggtccagct tgttttcttt tgctaatttc
     5881 gtcaaatcac gtgctaatga atctagcgta ggtgttcctt gcggtgtttg ccgtttatct
     5941 tgtacattta catttctacg acgattcatt ccttttccag cagaatcacc agctagcccc
     6001 attttttgtt tcagaacttg acgggctttt gaaaggctga tacctaagtt gatcatgatt
     6061 cgagtagcca tgatactttc atcttttaat aagctcatca aaatatgttc tgttccaact
     6121 ttttgagaac cagttttctt cacttcgctg ctagctaaag caaaaacttg tttcattctt
     6181 ggtgaatatg gaagaaagac tcctttaggg taagaacgga taccaccata accaatcaga
     6241 tgttcaattt cttcataaat ttccgtttca ttcaaatcca tttcacgtaa tacttttcca
     6301 gcaattccat tttgctcgac cactaaggct agtaaaacat gttctgaact aactgattga
     6361 tgtttcaacc gttttgcttc ttcttgtgca atttctaata ctgcttgggc tctttcagta
     6421 aaaagttctt ccatctaatc tcacttccca tcttcataac ttaaacgttc cagaaatgct
     6481 cggagaatcc gagcacgtgt atgatcttca tattcattga aattcaatgt gttttttgca
     6541 attgcactca acatcaagtt tccttctttt tttgtaatta tttggtcttc ataaagtttt
     6601 tgaataatag caaaagcatt tttctcactg attgtatcat caaataactg atttatctgc
     6661 tcaagtaatt gttttttatc tgaaatcctt acttttgcaa ttcgtatata accaccacca
     6721 ccacgtttgc tttctactag ataaccacgt tggatggtaa aacgggtatt aatcacatag
     6781 ttgatttgtg aaggaacaca gttaaagagg tctgccattt caatccttcg aatctcgatc
     6841 atttcactat tttccagaat tttctttata tatgattcaa ttaaatccga ggtattctga
     6901 tgtgccatct tattcattcc ctttgactaa tattgacctt aattataacg aatttgataa
     6961 tagaaagaaa gaagaacgct ttttgttaaa taaatccttt agaatcaaca aaaaaaaata
     7021 cggaatatac tattccgtat c
//
LOCUS       DACCWK010000116         6410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCWK010000116 DACCWK010000000
VERSION     DACCWK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.120"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(176..496)
                     /locus_tag="IVW65_002363"
     CDS             complement(176..496)
                     /locus_tag="IVW65_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901214.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(877..1995)
                     /locus_tag="IVW65_002364"
     CDS             complement(877..1995)
                     /locus_tag="IVW65_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901215.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2466..3773
                     /locus_tag="IVW65_002365"
     CDS             2466..3773
                     /locus_tag="IVW65_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7901216.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            3892..5091
                     /locus_tag="IVW65_002366"
     CDS             3892..5091
                     /locus_tag="IVW65_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901217.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5118..6386)
                     /locus_tag="IVW65_002367"
     CDS             complement(5118..6386)
                     /locus_tag="IVW65_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7901218.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         1968 a         1336 c         1165 g         1941 t
ORIGIN      
        1 taaaaaaatt attctttctg actacttatc ggttctgttt caacggtcga ggctggggaa
       61 gattctttag taggtctgat gttctcatgg tatttctgat agcctatgac agttaccaga
      121 agaacaaggc aaaccaatac gatcaacgag attagttttt cttttttgct catatctata
      181 cctcataaat aacaggatga tgtccatcat atttctgagt atgagtaaaa gcaaaagctg
      241 cagctgtaga atgtaagaaa tgccagcaac tttcataatc acaggtcgga cagtaccaaa
      301 agtaaccaaa tcttggctgc aaagaccgtt cttctgtttc aggaatcgca gaatcaagat
      361 ctggggtctg tacataaaca tttttgtaca gacgtgtgcc tgtaatagga gaccgctgtg
      421 tttcttcagc aaaaactggc tgacttgtta aacttaggga tagaaacagc aaacaccata
      481 acagtctttt tttcataaag acctccttat cgataagttt ttcccaatta aaatcatatc
      541 tcaagaacgc ccgttattca cattttgttc ataaatgatg ttttttgtat cataatcgtc
      601 aaaaagaggt cgtgaccacg tcacaacctc ttttttaaac ggtgttcaaa agcttgcatc
      661 tgcggtaata agcccagaaa aacgaaaaat aggaggagct atttttgttt ggctgttctt
      721 attacttgat gcagaacagc tttttcacaa cctctcatac acggtgttta atcagctgac
      781 cttcggtatt agctcaaaaa acggaaaaat atgtgaaact atttttccgt ttgtttctct
      841 aatactcagg tcaaaacact gatttcacaa cctcttttaa taacgaagaa ttgctgtcag
      901 actcttttca tctggcgcat ctttttgatc caaaataaac acttttccgc cattaatgat
      961 cacatcatct gctaatgcat tcaacacttg acgccgatca tattctgttt caggatcttc
     1021 accgaaacca tcagctaagt ttgacgtagc gataaataaa tgtgacactt ttccttcttt
     1081 cgcagctgga acgatatcaa ctaactgatc gatgaatttc ttatctaaga gtttattgta
     1141 ttcttttgat tcttgagctg cgagttcact tgacagcttc ttcgcccctt cttcgatatc
     1201 tctaaaggat agctgcgcag gagaagcaga aatcgcaacg tctgcacaat aatagggatt
     1261 tttagcaatt tttttgaaca tcgtctgatt ttctggtaga gcaaataaat aaatcggcca
     1321 ttgttctgga ttttcgaaag aatccttcaa gtacgtatca actgcttggt aatagttgac
     1381 ccaatcgatc tctacttctt catcttttgg atttacgcca tggaaagtta ctccatctcg
     1441 ggctccgcct cctccattag aagagaagtt caaaccgcca cctgtcagtt cagatcccaa
     1501 tgcagtaacg acatcaaccg gtgccccttc tggtagagat acttctttta cttgtttgtt
     1561 gctgacttca tacaatttca tggagtcgcg gttcaatccc aataaataat aagaatagtt
     1621 gaactgccca tttttgataa tagccagtaa ataaggaaga tcacttacat agtattgatc
     1681 gtctaccggg atactcaaac gatggatata ggtttggtca gcagttataa tgaccgctac
     1741 actcgtagtt gcactacgcc aaaacgaagg atctcctaac aaattttcaa ttttttcttg
     1801 gaacggggac cagtcatgtt cggaatattt tttctcaaaa cgtttctttg cttctttcgc
     1861 aaaatttttg aatttgatct gatctttttc cacatcttga tgggcgacat gcgtattcag
     1921 catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc aattgtgaca ataattcttt
     1981 atcctttttt gccataaacg tacccctcct aaaaaaatca ttcctttgtt tctatctaaa
     2041 gtctaccaca acactatttt tccatacaat aaaacgtatc taaaaaaata aaacggtttc
     2101 tacggtttta aatttagcta tttttttaag ttatcttcat aatttcctca attatttagc
     2161 cccagtcttc aactattctt catgaaatgc tgataaacta agaaaaagaa taaaagaggt
     2221 ggatcttatg catttgatta tgattttcag tttattaagt attatcagtt tatttgcttt
     2281 cagcgcttcc cgcttattag atgcggaaac acagcctatt cctgtaaaag tacgtaaaaa
     2341 cgaccgctaa ccttccataa cgtgcgttct tatcctaaag ttttttcaga aacattgaaa
     2401 ctctctcccc tctctgataa aattttaatt tgtagcgaaa caaattaatt acaaggagat
     2461 cggatatgtc cataaaaaaa acacgtctag tcctattatt ggctagtctc attctttgcc
     2521 ttgcttcttt tctaccagct gttgctgtgc aagctgaaga tacgttcaaa gtaaatgcaa
     2581 aagcagcttt tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa
     2641 aaaccatggg gatcgcttca atcacaaaaa tcatcggtct ttacatcgtt cttgatcaag
     2701 taaaagaagg aaaactatca tgggatgata aagtttcgat ttcagattat gcagaaaatt
     2761 tgagtatcac cccagattta tcgaatgttc ctttgcataa agaaaacacc tacactgtca
     2821 aagaattatt cgactctgca atcatccagt cagccaatgc ttcaatggtg gccttggcag
     2881 aaaaaatctc tggcagcgaa gctaaattca ctgaacgaat gaaagaacaa ttgaaagatt
     2941 ggggaatcaa agatgcaaca atcgttaatg catcaggatt gaacaactct tatcttggcg
     3001 aaaaccgacc agaaggtact ggagaaaacg acgaaaatca aatgtctgct caagatgtgg
     3061 ccatcgttgc ccgccatttg atcttggatt ttccagaaat actggacgta tctagcacta
     3121 cgacccaaat gtttggcgaa aacacgcaat caccagtgga aatggtcaac tggaactgga
     3181 tgctcccagg attcgttaat tacaaggaag gcgtggacgg attaaaaact ggtacgactg
     3241 agcttgcagg tgcttgtttc gttggtacga tcactaaaaa cgatcaacgg atcatcactg
     3301 tcgtattgaa tgcggacggc catgcggaaa acccgagtgc ccgcttcaat gaaacagacc
     3361 gtttgatgga ctactgctat gacaattggt cagaaaaaga gctcggaaaa gctaacgcaa
     3421 gtattcctag cctaaaaacc attgatgtaa aagacggaaa agaatcaaca gttcctgttg
     3481 tgcttaaaag ccctgtaaaa gtatgggtac gaaacgatat ggatactggc aaattaacga
     3541 tcacgccaac acttgacaag aagcaagtca ctgacaacga gatccaagca cctatggata
     3601 aaggtacaaa aatcggtacc gctacgatca ccttggcaga cgataaatta ggttatttag
     3661 aagacaacaa tgcgccatcg acagagatca ttacggataa aacagtggaa aaagcgaata
     3721 tgttcgtgat tggctggcga cgcgtcgcag aatttttcgg caacttgttt taattgaaca
     3781 ataagaaaag catctttcca tcggaaagat gtttttttgc ttttactagt ttaaataaca
     3841 gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt tatgaatcta
     3901 accagagatc tgatcttttt agttctctat cttattgggg tattctctat cgtttattgg
     3961 cgaaaacaga agaaggatcc ttccttgttc ttttacggca gtacgtggac atttacggta
     4021 atcttaactt attttgtcgt gttggaaatt ttttattcga ccttttattt tttgattcca
     4081 ttgttgttct ttagtatctt tgctttttct tatttcacag aaaaacgaag gctgctgaat
     4141 ggtcttttat tcaatgtttt cctcatcagt ttcggcatct acttgtttgt cttgctgtat
     4201 gaaacgcaaa atattttcct tggcggactg atcgccttga tcacgattcc attgctactt
     4261 gtccttttat ttggtattta tggattgatt gtttttcttt tttggaacgg ggtcacagtt
     4321 ttacgtagag aatcgcattc attagctaat cttttaacgt tgatactagc catttttctt
     4381 acactgtttc ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg gataaatgct
     4441 ttgtttttct gcgttcccct gatcttgatc tatctattta tcgtattcta caactttttg
     4501 actgtctcat tcttgtatca gttcaaccga cctcgataca atcaagactt catcgttgtg
     4561 ttaggcgctg ggttgatcaa tggagaaaca gtgtctcctc ttctagccaa gcgaatcaat
     4621 aaggcaattg ctttttatcg ggctcaaagc cgtgctacat taaatccgcc catcttattg
     4681 atgtctggcg ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat gaaacaatat
     4741 gccatggaac aaggaatccc tgaaagagac attcttgtcg aaaccaattc gacaaccact
     4801 ttggagaaca tgctctattc aaaggaaatc atggatcagc aaatgaaagg gccataccgc
     4861 gcgatcttta gttccaacaa ttaccacatt tttagagccg gtctttatgc gcggcaagcc
     4921 aaattgaaag caaatggtat tggtgcaaaa acagctttct actacttgcc taatgcgttt
     4981 ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat catttgcgga
     5041 ttgcttttcg caggctccgc attcttatcg ctgattactt tacttttcta aaaaaacaag
     5101 gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac tattggcaaa
     5161 aagagtatcc cttttctgac cgctacttgc tgagcagaat aaataccgct gttgcctgaa
     5221 cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga accgaacaat
     5281 tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa tactccgata
     5341 aagcccaatc ctgcaagaaa aggaacagaa acatggagca aaggcgctag tgctgcacca
     5401 agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc cgcgcctaaa
     5461 gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg tccattaaat
     5521 gattcttgaa gtaatggaag actcaatccg agataacgtt gtgtttgcaa aatcaatgct
     5581 gctgccacaa ctacagcccc acctatcgta tttcgccaaa caggatgagg aatccattgt
     5641 gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat caaaccaaaa
     5701 gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg aatcacaccc
     5761 atttggtaat gtagatgaga aacaccatag ctttctgtca taaaatttgc aaacagtgca
     5821 gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa cacttctaat
     5881 ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc actgattcca
     5941 ctgataatca agatttcccg ttctgattta tccaatcgga acaaacgtgc tgtattatct
     6001 gctaacgctc cccccatttg gactgctgtt ccttcacggc caacagatcc tccaaataaa
     6061 tgggtagtta tggttccaaa cagtgtaagt ggaataagtc gcaacgggat tttttcttct
     6121 ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc gccgtgatac
     6181 gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag ccaaggatgt
     6241 gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga taagctcccc
     6301 atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatgtacac taaaatgcgt
     6361 actggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
//
LOCUS       DACCWK010000117         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCWK010000117 DACCWK010000000
VERSION     DACCWK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.121"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            725..811
                     /locus_tag="IVW65_002368"
     CDS             725..811
                     /locus_tag="IVW65_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7901219.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVW65_002369"
     CDS             complement(932..1621)
                     /locus_tag="IVW65_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901220.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IVW65_002370"
     CDS             complement(1628..1906)
                     /locus_tag="IVW65_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901221.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IVW65_002371"
     CDS             complement(1866..2780)
                     /locus_tag="IVW65_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7901222.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVW65_002372"
     CDS             complement(2762..3142)
                     /locus_tag="IVW65_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7901223.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVW65_002373"
     CDS             complement(3592..3879)
                     /locus_tag="IVW65_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7901224.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVW65_002374"
     CDS             complement(3899..4123)
                     /locus_tag="IVW65_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7901225.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVW65_002375"
     CDS             complement(4763..5296)
                     /locus_tag="IVW65_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7901226.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVW65_002376"
     CDS             complement(5317..6159)
                     /locus_tag="IVW65_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7901227.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCWK010000118         6016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCWK010000118 DACCWK010000000
VERSION     DACCWK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.122"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(57..359)
                     /locus_tag="IVW65_002377"
     CDS             complement(57..359)
                     /locus_tag="IVW65_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901228.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(579..945)
                     /gene="ssrA"
                     /locus_tag="IVW65_002378"
     tmRNA           complement(579..945)
                     /gene="ssrA"
                     /locus_tag="IVW65_002378"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1116..1889)
                     /locus_tag="IVW65_002379"
     CDS             complement(1116..1889)
                     /locus_tag="IVW65_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7901229.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2033..2383
                     /locus_tag="IVW65_002380"
     CDS             2033..2383
                     /locus_tag="IVW65_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7901230.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2364..3080
                     /locus_tag="IVW65_002381"
     CDS             2364..3080
                     /locus_tag="IVW65_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7901231.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3080..4528
                     /locus_tag="IVW65_002382"
     CDS             3080..4528
                     /locus_tag="IVW65_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7901232.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4598..5107)
                     /locus_tag="IVW65_002383"
     CDS             complement(4598..5107)
                     /locus_tag="IVW65_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901233.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5097..5822)
                     /locus_tag="IVW65_002384"
     CDS             complement(5097..5822)
                     /locus_tag="IVW65_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901234.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2047 a         1048 c         1170 g         1751 t
ORIGIN      
        1 ttttataagt atggtgataa aatccgttcc gggctacctt ttttaggtag cctactttaa
       61 tctttatacc tttctggatc aacgaaagta tacttcaaat agtcataacg ccgatgatcg
      121 ctccgtgcgt ctggcacatc agtcacgata tcaaacaaaa aatacacatc tttcttcatt
      181 ctggtttttg ttgccggaat tttaaagtag ttcttattag agtaatagag attgattaat
      241 aagctatctt cgattgctaa aaagaaaact tctgaatccc acaccttata aaaatctttg
      301 acaaatctat tcgaaggatc aaatttaaac cataattgtg tttttccttc catcaacata
      361 atgttcacct caaaaagagt atacgaacta atgttctttt tgtaaagtag gaatatcatt
      421 gataatatgg atggaaaata tatattaaaa agtgttcttt ttgaatgcta ttttgaatgc
      481 cattttcatt catttaacga aaatctgcga aaataaaaaa tgctcaaacc cttataaaat
      541 aaggatttaa gcatctagcg aaatctgctg aaatctcaca atggagacgg cgggagtcga
      601 acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga
      661 ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt
      721 tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca
      781 catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt
      841 gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa
      901 acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg
      961 ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa
     1021 gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat
     1081 tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat
     1141 gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat
     1201 ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca
     1261 ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga
     1321 cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca
     1381 ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat
     1441 aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca
     1501 tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt
     1561 ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt
     1621 tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa
     1681 gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct
     1741 gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata
     1801 gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca
     1861 aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt
     1921 tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg
     1981 ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt
     2041 tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa
     2101 aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg
     2161 gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt
     2221 gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct
     2281 gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga
     2341 cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt
     2401 ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg
     2461 gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag
     2521 acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa
     2581 gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct
     2641 tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc
     2701 gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagcagttt
     2761 cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag
     2821 cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta
     2881 ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg
     2941 attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa
     3001 aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg
     3061 atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tctgcggaat
     3121 ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat
     3181 tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca
     3241 aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct
     3301 gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga
     3361 acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg
     3421 gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca
     3481 agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc
     3541 acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa
     3601 cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc
     3661 agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg
     3721 taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga
     3781 tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa
     3841 tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat
     3901 caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc
     3961 gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga
     4021 ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga
     4081 aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt
     4141 gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat
     4201 catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac
     4261 tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc
     4321 tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa
     4381 tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca
     4441 taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt
     4501 gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat
     4561 cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     4621 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     4681 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     4741 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     4801 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     4861 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     4921 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     4981 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     5041 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     5101 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat
     5161 agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt
     5221 atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc
     5281 ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc
     5341 tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg
     5401 atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata
     5461 aaaaggaata atttggtttt agaaagacca gtcaggcgcc aattgatcaa ttcctgcttg
     5521 ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa
     5581 ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta
     5641 gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc
     5701 aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa
     5761 tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc
     5821 atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt
     5881 tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa
     5941 gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga
     6001 gcagcgacaa taattt
//
LOCUS       DACCWK010000119         6004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCWK010000119 DACCWK010000000
VERSION     DACCWK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.123"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..402
                     /locus_tag="IVW65_002385"
     CDS             <1..402
                     /locus_tag="IVW65_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901235.1"
                     /translation="GYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELY
                     KEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDS
                     NAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            416..1105
                     /locus_tag="IVW65_002386"
     CDS             416..1105
                     /locus_tag="IVW65_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901236.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1230..3581
                     /locus_tag="IVW65_002387"
     CDS             1230..3581
                     /locus_tag="IVW65_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7901237.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3661..4056
                     /locus_tag="IVW65_002388"
     CDS             3661..4056
                     /locus_tag="IVW65_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901238.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4381..4998
                     /locus_tag="IVW65_002389"
     CDS             4381..4998
                     /locus_tag="IVW65_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901239.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5039..5338
                     /locus_tag="IVW65_002390"
     CDS             5039..5338
                     /locus_tag="IVW65_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901240.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5372..5539
                     /locus_tag="IVW65_002391"
     CDS             5372..5539
                     /locus_tag="IVW65_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7901241.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5584..>6004)
                     /locus_tag="IVW65_002392"
     CDS             complement(5584..>6004)
                     /locus_tag="IVW65_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901242.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2066 a          828 c         1209 g         1901 t
ORIGIN      
        1 ggctattttc ttttgaattt agattctgtt aattcaaacg ctcaaataaa agatcaaaga
       61 gtagctgaac ataataataa aaaaatgagt ttgaccaaaa atatctattc tatgtcagat
      121 gaagaattgt ataaagaatt tggtgattta tccggggaac agttagaaga aagtattcca
      181 ggaattagtg actcagataa aattgttgtc ttgtctgctg gtgggcaatt aatgggagat
      241 ggcaaatccg aattagttag ttataaacaa aatcacagac agtttattga tgttaatact
      301 gattccaacg ctatttcgat taaggatcta aaagagcttt taaatagtaa aagaaatgaa
      361 ttaaagcaga taaaagaaac taattcagtg gaattagagt aaaggaagat tcattatgaa
      421 aaagattttt ggtgtttctg ttgggctact tatgatgtta acatttggaa atgcagtata
      481 tgctgctgag aaagaagtta tttcagatcc agagcagtac gcctcacgat actttgctga
      541 ttttgaagaa gaagataaag tacttgcatt gccagaaggt ggttttttaa aaggggaagc
      601 taaaattgta tctatggata atccagatat aattcaaggt tcatataatt cagaaactga
      661 tcctgcttca gtttcagtga cagaagcaaa aaaaattgtt gctgaaaaat tcaaaaatga
      721 tattgaaact actccaatta tgcctcgagg agcatttcca cccgatgttg gtactagcgg
      781 acaccttctt gtactagcag gaggaggaag ctataaatca cagccattta gtggtgcggg
      841 atggcgtttt ggaggtatgg gtgtaaaacc agctgatggt acaggcaact ttttacgttg
      901 gaaatcctac attgacgatg gaagagtagg aaatgtaaat caagccaacg caactaaatc
      961 tggttctatt caaggaatgg cattgtatgc tcgtgaagga ttcaaatact ttgcaccaaa
     1021 cgggcctggt tggacaatga atataggtat ttattacact tataatcctg ttgcaggtac
     1081 gtattatgtt gttcaaaata tttaataaaa cagcacaaag agatttcttt gtgctgtttt
     1141 attattttta gttgttgaca aacactaaaa tataaaatat aatcaacaca taaacgaacg
     1201 tatgttcggc aagaaagcag gggattttta tggatgagat ggttttaagt actcagaaat
     1261 ggctaaacaa gaaatacagt aatgttacag ggtttgataa ggttccagaa aatgggcgaa
     1321 cagggtggcc tacaatctat ggacttattg aaggactaca agtagaactt ggtattacta
     1381 atctagtagc taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta
     1441 aatggggcaa aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta
     1501 aggggattaa tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa
     1561 aagagttgca gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga
     1621 aatgggccaa agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta
     1681 aaattagaaa aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc
     1741 cttgtgatgg tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg
     1801 aactaggata ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa
     1861 cttatcctgt gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat
     1921 atgttaatgg ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt
     1981 tagaagtact ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg
     2041 ttgttgtaat aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg
     2101 cagatacttc tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa
     2161 aaatagttgg tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt
     2221 atctaactac agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct
     2281 atcaagatgg aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta
     2341 agaaggctat agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg
     2401 ctgttgatct agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg
     2461 gggtatctgc tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg
     2521 tttgttctca aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga
     2581 ctggttatag tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg
     2641 ttgaatttac cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg
     2701 gtagagatag cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca
     2761 aatacaatca gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg
     2821 gtcttgataa tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt
     2881 tgtcatggga catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat
     2941 ataggaataa attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg
     3001 gttcaataat aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg
     3061 acacaatcaa tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct
     3121 ggggtgaagt taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta
     3181 cggctgcagg tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg
     3241 tttatacctt agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact
     3301 attgtgataa gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt
     3361 cagataaaat cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct
     3421 taggtgtagg gataatttca tttttcattg ataaaatagt taataattca cttgggacag
     3481 attttactta tcttgatttt attggagatg aggggaaagt tgcaaatgga ttttctgaaa
     3541 aaattattca atttcaagaa aatagaagta aacttaatta agaatcgttt actatttaaa
     3601 cagtgtcctt ttccttttta aatatatacg ataaaataag tatatgaatt gtaaaaggac
     3661 atgatgatta tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct
     3721 ggatgtggaa ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact
     3781 gtcaagatag tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct
     3841 gagtttgaaa gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta
     3901 aaagagtctc caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa
     3961 aaaaagaaag aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac
     4021 cctaaattag aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt
     4081 taaagaagtc aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa
     4141 ctggataact agtttattta atggcagacc gttaaggcct gccattttta tgtattttag
     4201 cggaataaat ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga
     4261 catgccttga ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg
     4321 tttctgattt aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg
     4381 atgagaaaat ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg
     4441 atttccgcga aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc
     4501 aaagatttta aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta
     4561 ctctatctac aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg
     4621 cgagaattaa ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat
     4681 actgctagtt acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa
     4741 ttactcagct atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc
     4801 gtagaagctg cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct
     4861 gaatttcctg cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg
     4921 gatcaattgg gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc
     4981 ttgcaccact tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat
     5041 ggacttaacg aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca
     5101 agccatcggt ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac
     5161 aaatttcctg gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa
     5221 aaaggaacaa accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga
     5281 agtcgaattt tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc
     5341 gttagtaaaa tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg
     5401 cattgaaata actagtttta atcgaaatat tgcttttcac gtggagtgtg actgtgggga
     5461 attagcaaaa agtgtttatc aaaaaaatta cttttactgt cctaaatgcc atcgccgata
     5521 cggggtacat ggaacataga aacgaaatag tgcggtagaa aagcgaatga atgtagtata
     5581 tttctacaaa tcattcgctt tctttatttt ataaatagtt ggtcgagaga tcccgttttc
     5641 ttttgcaatc tctgcaactg gaatgccttg ctttaacatg tccaccacgg ttttaaaaac
     5701 taagcgtttt tgcggatctg ttgagttaat agaatactcg attggtcgtc ccttatatcg
     5761 gccggctttt tgagccaata atactccttg ccgctgacgt tcacgaattt tctcacgctc
     5821 gttttgtgca atataactta gcagtgataa aaacatatcg aacatagcgg tatccaggag
     5881 ttcatttcca gtattaaaat ctaaaaactg tgcatctaat atttttaacg ctactttttt
     5941 ctgtttcata aaagcaactg tattttttat atcttcataa tctcttccta aacgatctag
     6001 tgat
//
LOCUS       DACCWK010000120         5864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCWK010000120 DACCWK010000000
VERSION     DACCWK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.124"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            690..1658
                     /locus_tag="IVW65_002393"
     CDS             690..1658
                     /locus_tag="IVW65_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7901243.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IVW65_002394"
     CDS             1669..2484
                     /locus_tag="IVW65_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP7901244.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IVW65_002395"
     CDS             2552..3262
                     /locus_tag="IVW65_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7901245.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..4539
                     /locus_tag="IVW65_002396"
     CDS             3298..4539
                     /locus_tag="IVW65_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP7901246.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNESSPQSCVKVQPHQKH
                     NVLRCEPNCHAFLSEERGQEQ"
     gene            complement(4597..5784)
                     /locus_tag="IVW65_002397"
     CDS             complement(4597..5784)
                     /locus_tag="IVW65_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7901247.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1908 a          974 c         1175 g         1807 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaatgagtc aagtccacaa
     4441 tcttgtgtaa aagttcaacc tcatcaaaag cacaatgttt tgcgttgtga acctaactgc
     4501 catgcctttc tttctgagga gagaggtcag gagcagtagc gcagcgactc ttgatgtctc
     4561 tcctcagaaa gaagagactc gcacaggttc atggtgctaa atgttattcg aattaaattc
     4621 taaatatttt cgtggagaag ccagccactc agtgtctttt tccatcaaaa gtgttccaat
     4681 tatccgagtg atggaattga tatttggaaa aatccgaatc actcgttctc tccggcgaat
     4741 ctcttcgttc aaccgttcca acacattggt tgtacgaatc cttcggcgga tattttcagg
     4801 aaaagacaaa atttgaatag catcttcaaa gccgttttct aaaatgtcgc aagcagcact
     4861 taattttgaa tcacaaccat atttttccaa gaagtgctct tttctttcac gtgtcaattc
     4921 aagttcagag gctttaaaga tactcttcaa ctcgtctttt acatcagaag aaactttctt
     4981 tggcagctta tcaaaaatat ttctcaaaaa gtgggcttgg catctttgcc agctcgcacc
     5041 taaaaagttc ttgcgaacag ccttaactaa ccctttgtgt gcatcagaaa tgaccagttt
     5101 tacaccgaac aaacctcgtt gaacgaacga ttgaaaaacg gtatcccaat tatcttccga
     5161 ttcgccgtct tgaacatcaa aaccaagaat cgtacgtcgt ccatcagaat caatgccgat
     5221 accaatatat acaccttttg agacaatacg atgattttcc cttactttca tatacagggc
     5281 atcacacatg aaaaatggat attccgagcc ttctaggctc cggtttctcc actcaaaaac
     5341 aaatggatcc aactgtttca tcagatttga aacgaatgat ttagaaacag tcgcaccatc
     5401 cgttagcagt tcaactgttt tagttacttt gcgagtggag acaccagaaa tgaccatttc
     5461 aatcatggca gcgatcaaag ctttttcgct tcgctgatat ctttcaaaga tagtaggact
     5521 aaacttaccg tctctggttc gtggaacatg aagttctaac gtaccaattc gagttgtaaa
     5581 cgaacgatcg tagtaaccat ttctttgtga ttgccgttct tctgaacgtt cataggcatt
     5641 tgcctgaata tattgatttc gttgttcttc catcagctga ttaaacatga tagtcataag
     5701 tgtttttgcg gtatcattcg caccgctttc gtttataatt gcttgaattt cttcgctttc
     5761 aaaagtaaaa tgtacttgag tcattgatat cagtcctatt cgagtatgtt ttctgcaaaa
     5821 aacattgtac tcctaactga gatcaatggc ttttttgatg cttc
//
LOCUS       DACCWK010000121         5707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCWK010000121 DACCWK010000000
VERSION     DACCWK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.125"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(93..764)
                     /locus_tag="IVW65_002398"
     CDS             complement(93..764)
                     /locus_tag="IVW65_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901248.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(757..1251)
                     /locus_tag="IVW65_002399"
     CDS             complement(757..1251)
                     /locus_tag="IVW65_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7901249.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1271..2491)
                     /locus_tag="IVW65_002400"
     CDS             complement(1271..2491)
                     /locus_tag="IVW65_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7901250.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2638..4473
                     /gene="lepA"
                     /locus_tag="IVW65_002401"
     CDS             2638..4473
                     /gene="lepA"
                     /locus_tag="IVW65_002401"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7901251.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4567..5694)
                     /locus_tag="IVW65_002402"
     CDS             complement(4567..5694)
                     /locus_tag="IVW65_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7901252.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1745 a         1145 c         1084 g         1733 t
ORIGIN      
        1 ttttttcaaa taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt
       61 gaagctgccc gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta
      121 taactttcaa agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag
      181 gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg
      241 acaaacagtg tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg
      301 cataagaatt cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat
      361 aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc
      421 aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa
      481 aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa
      541 aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact
      601 tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga
      661 gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca
      721 aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc
      781 taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa
      841 tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg
      901 tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc
      961 gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat
     1021 cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc
     1081 aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg
     1141 aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt
     1201 atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc
     1261 atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta
     1321 gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac
     1381 ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta
     1441 taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat
     1501 tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa
     1561 acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga
     1621 tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat
     1681 ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc
     1741 gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt
     1801 agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat
     1861 cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat
     1921 ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc
     1981 gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg
     2041 aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat
     2101 acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat
     2161 cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca
     2221 ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca
     2281 gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac
     2341 ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga
     2401 acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt
     2461 caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa
     2521 cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa
     2581 ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg
     2641 gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat
     2701 atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg
     2761 tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc
     2821 acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc
     2881 tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt
     2941 gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca
     3001 ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa
     3061 atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga
     3121 atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt
     3181 ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa
     3241 gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc
     3301 acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt
     3361 gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc
     3421 ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat
     3481 acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat
     3541 ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa
     3601 gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa
     3661 gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa
     3721 gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac
     3781 catgttaata aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa
     3841 cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat
     3901 gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg
     3961 gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc
     4021 tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa
     4081 atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa
     4141 gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc
     4201 gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct
     4261 attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct
     4321 aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg
     4381 aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt
     4441 ctgaaaatgg acgaggacga accgaagaaa tagcaagggt ttgtttgaaa acagtgtaaa
     4501 aaataaacca tgtttacatg gatttgccac cggtttgcca ccggtggttt ttttcggtgg
     4561 caaaaatcaa gaattctttc taagattttc catgatatcg accgtttcat ttctcatctt
     4621 atctgttaca tgcgagtaag tatccatcgt aattgaaatt ctgctgtggc ccaatcgttc
     4681 agagatttct ttcatttttg cgccattttc gagaagaagt gtagcatgag tatgtctgag
     4741 agaatggaag ttaaaagaga gggagagtgc attcgatatc ctcctagtat tccatttcac
     4801 tacacttggc gtaactagct caccgtcttc cttcgtacat actgcatttg aatcaatgta
     4861 gagctttcca tacttcattc gattttctaa ttgttgtttc ttatgttttt tcagaattgc
     4921 tagcaaggtt tgtccaataa aaatcgttcg attggaactg cttgtctttg gcgtaccata
     4981 tacccatgcg ccgtcattct ttaccatttg tttctctaca gtaattgttc catttgaaaa
     5041 atcgacatca tcccacgtca gaccacaaac ttcgccaacg cgcattcccg tataaaatcc
     5101 aatattcaaa ggaatataga aaggatgtcc ttcaggagta atttctagca tatgatcaaa
     5161 gtcctcaaga gaaatgattt ttagatcttt tttagtcgtt ggtcgtgctt catattttgg
     5221 tatttttaca tacagcatag gattttgctt gattaatccc caaggataaa ccgccatatt
     5281 cagcgcattc ttaaggacag agtgagtaat agtcattgtt ttcttcgagt aacccttttt
     5341 aaattcagca ttgatgaaat tttgtaaaag agcaggggag agatccgtaa gttttttctt
     5401 tcctaaataa ccgtttatat gatttttgat ggtaaatcgg tagttttcat aggtattgta
     5461 ttttagattt agtttaacgt attcctccat ccaaaaatca aggtattgtt ttactcgagt
     5521 atccgtacct aaaaagtatt gtcctgtttc gtcaatatct gataaaactt ttcgtaaagc
     5581 agcttcggcc tctggtcggg tgtctccgcc aactttctcc actttttttc ttgagccatc
     5641 atcattgata tcttcgccgt ttgtataatt aagcgagaca aataaaaaag ccatttatgt
     5701 tacactc
//
LOCUS       DACCWK010000122         5689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCWK010000122 DACCWK010000000
VERSION     DACCWK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.126"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(488..1297)
                     /locus_tag="IVW65_002403"
     CDS             complement(488..1297)
                     /locus_tag="IVW65_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7901253.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="IVW65_002404"
     CDS             complement(1297..2043)
                     /locus_tag="IVW65_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7901254.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="IVW65_002405"
     CDS             complement(2061..2528)
                     /locus_tag="IVW65_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7901255.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="IVW65_002406"
     CDS             complement(2528..2938)
                     /locus_tag="IVW65_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7901256.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="IVW65_002407"
     CDS             complement(2949..3962)
                     /locus_tag="IVW65_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7901257.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="IVW65_002408"
     CDS             4179..4913
                     /locus_tag="IVW65_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7901258.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="IVW65_002409"
     CDS             4897..5607
                     /locus_tag="IVW65_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7901259.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DACCWK010000123         5629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCWK010000123 DACCWK010000000
VERSION     DACCWK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.127"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(139..546)
                     /locus_tag="IVW65_002410"
     CDS             complement(139..546)
                     /locus_tag="IVW65_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901260.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     KNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(565..1383)
                     /locus_tag="IVW65_002411"
     CDS             complement(565..1383)
                     /locus_tag="IVW65_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7901261.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(1609..1902)
                     /locus_tag="IVW65_002412"
     CDS             complement(1609..1902)
                     /locus_tag="IVW65_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7901262.1"
                     /translation="MSINTFVYSHPINVYIIKYLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFTSNQQRKVKKQID"
     gene            complement(2118..2468)
                     /locus_tag="IVW65_002413"
     CDS             complement(2118..2468)
                     /locus_tag="IVW65_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901263.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANNNLAKEQMTTE
                     EISEVLNAAVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKYKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(2461..3786)
                     /locus_tag="IVW65_002414"
     CDS             complement(2461..3786)
                     /locus_tag="IVW65_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7901264.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRQKLAQMIQEHIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCFFLFDKYYEGQEVRHVGITYSKLIYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTAIVHASSMLEGARSITRSTLVGGHAGGNGGLKND"
     gene            4236..4850
                     /locus_tag="IVW65_002415"
     CDS             4236..4850
                     /locus_tag="IVW65_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901265.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(4874..5224)
                     /locus_tag="IVW65_002416"
     CDS             complement(4874..5224)
                     /locus_tag="IVW65_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7901266.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(5218..5481)
                     /locus_tag="IVW65_002417"
     CDS             complement(5218..5481)
                     /locus_tag="IVW65_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7901267.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT         1702 a         1035 c          845 g         2047 t
ORIGIN      
        1 ttttgtaatg ttgatttaat cgcaaattta cacgttgttt attaagttat accttaaata
       61 tttaatgtat aacttatact ttaatattca tttatctagt ataacttata ctttaattga
      121 ataccgcatg aaaaaaaatt attttggtaa tttttcaaac tcttcttgta tttcttctct
      181 tatgtgttct tcttttgcta ctcctagtct atgctggata tatgagtctg ctaatgtatt
      241 aacaattttt tcgaaagaaa ttcttccttt ttcactttgt aactccgtca tatagtcttg
      301 taaaattgag agtttgctat ttaatgccgg agagatcttt ttaggaactg ataccttgtt
      361 tcttttatca ggaattgaaa ctggtcggcc tctttttttc aatgtaatat tttttgtgtt
      421 actcaatgca ctattgctaa agttatattc accgcttatt ttcacatctt ggcgtttatt
      481 attgggtaat aatggctcaa attcgccatt ctccatcatt ttaggaatat caaaatcttt
      541 aggcattact tatgcaactc cttattattt atttaaaaac tcttgtctag catctagttc
      601 tgccaaaata tttataaata cttggtgtgc tttataatcc cattgatcaa aatttccatt
      661 tttgtatttt cttaaatgta taccaatttt agaatacctt ctcaatcttt tttgataatg
      721 aatgagattt ttggatacca cggttccgta tttttcaatt gcttcttcta ggatttcttc
      781 atcaaaatca tcatttggtt ctaccataca aggtacaata tctataattt gcaaatcaag
      841 atcgtatcta tcgaccatga atttttgata atttacatat ttgttgactc cctctaagga
      901 ttcttcagct gtttgaaatg aaattatcga atagtctgaa gctgccatag cattatcgga
      961 aaattcgcta atggttggtg gtacatcaat aaatatttct tgataattgt tctttattgg
     1021 aagaagtaat tttttcaaga cagtaacttg gtctaattca ttttcatgtt tattagcaaa
     1081 agcggagaat gatcgaaaac tagtgtcaca tgccagaagg tcaagatttt catttacctt
     1141 aataatacta cttgctaatt ctccatttgc gacaccattt gttatagtgg ttttgatatc
     1201 ggttataccg aaagttcttg ctatgacttg tgttaagttt gcttgagggt ctaaatcaat
     1261 aataagcgtt tttctatttc ttatcattgc attgtcataa gcaagcatgc tgactatctt
     1321 agttttacct actcctcctt taaaattgcc aactgtaaat gtagtccctt gaaaagtatc
     1381 catgtaaatc ctcccaatct ttaattgata attaaagtat aacttatata tcggaaatgg
     1441 tcaacattaa aagtataact taaataataa tatctaaaaa tgtttggata tagcggtttt
     1501 taagaataaa agtataagtt ataccatata tatttagggt ataacttata cttttattgt
     1561 ttaaggtata acttatacat taaatgttgc ttttcgaaaa tagctattct agtctatctg
     1621 tttctttact tttcgctgct gatttgaagt gaattgatca tattcatttt ctagtattaa
     1681 taacttttcg tatacatcgg tcatattcat actagaagca agacttagat catatacaaa
     1741 tgaagcaggt aatgatttta ttctacgttg tcgggttatc cagcttgcaa cagttccttg
     1801 tgggtaggca tataactcac aaaattgttc tacagtgatt cctaaatatt tgataatgta
     1861 aacattgatc gggtgcgagt aaacaaatgt attgatagac atggtgcgta agcctacttt
     1921 cttttattat tgcgtttgca ataatagttt aacacacgca ttattctagt atctactcct
     1981 atttgtaaaa acaaaaaagg attcatagag ataactctat agaatccgta aatttatcaa
     2041 acgttggcca taaccccaaa tctcttctga tagctgatca aaggttatat actttagctc
     2101 atattgctgg ataatgttca ttctaatcga ctccatttat tatcctggta aaattcgata
     2161 tgacgtattt cgtcgtagtg aattttgtat ttgccaacgt agatccccag ctcatcacta
     2221 cctaagatct ttccaacaat atcgtctgga taatatcctt cagcgtttcg ttcttctttt
     2281 tgaatacata ctgatctatt ttttatgact gcagcgttca atacttccga aatctcttca
     2341 gtcgtcattt gttcttttgc caaattatta tttgcacgtt gctcattgtc cgcatcgatc
     2401 tcttccgtgt gatcggacaa atagaatcct gcccatttca accgtcctcg atcgttataa
     2461 tcaatcattt ttaagccccc catttcctcc tgcatggcca ccaaccaacg tgcttctagt
     2521 aatactacga gcgccttcta acatagaaga agcatgtaca atcgctgtaa aaccatattt
     2581 ttgtctgatc ttatcaataa tcttatccag gttttcttca tcgatttgtt tttgcggatc
     2641 actaaataaa tccagctgaa gcgaatctgt ataaattagc ttgctatagg ttattccaac
     2701 gtgccttact tcttgtcctt cataatactt atcaaacaag aaaaaacaat aattctgcaa
     2761 ttcctttgta ttatctgtga tagggatttt catctggtgt gaaaatcctg gtctcgtttc
     2821 taagatcgac gtaccaatgt ttaaatgaac gcatcctgtt ttacaattat gctgtctgat
     2881 acgaatggct acttgttccg ccatttcttt taccaccagc tcgatttcat ttcttctagt
     2941 ataatctcgt ggtaacactt gtgaattacc ataagatttt tcctttgtcg gttctggtgg
     3001 gatcgctata tctgttcgat cgattccgtt agcatggaaa tacagctgta atccaatcac
     3061 acctaatcta ttcttaatcg tataaggatt ccagttagca aggtctctaa tgctcaaaat
     3121 ccccatttgg ttgagccttt ttttcattcg cgaaccaatg ccccaaaagt ccgtcatttc
     3181 aggaatattc catacttttt caggaacgtt ttcgtacgtc cattccgcaa caaatccttc
     3241 attatgcttt gcttcattgt ctaaggctag tttagccaaa aggggattat cgccgacacc
     3301 tacagtagcg atcaatccta attcttcttt aatatgttcc tggatcattt gagcaagttt
     3361 ctgcctgcgc tgacttcttg ttccttcagt cgtaaaaaga ttcagtgatc tggtcacttt
     3421 taagatcgat tcatcgatcg aatagatcaa taaatcttcg tctgctacat accttctgaa
     3481 aatattgttt acctgcatgt ttcgcttaat atagagattc atgcgtggtg gaacgacatg
     3541 taacgtttta ggaaaaggct gtggtagatc ccgtggtctg ctgacattag aaataccgta
     3601 cttcttcttt gcagctggtg aagaagctaa aatcaaccct gatccagtgt tgtcagcacg
     3661 actcataaca acaagttctg ttgttaatgg atctaagttt ctttctatac attcgacact
     3721 tgcgtaaaag cttttcatat cgattaaaaa gtaatcattt actggctctt ttgagtaatc
     3781 catcataaat aacactccat tctttcatat tatacaaaca tatgttcgat attgtaaaga
     3841 agaaatagct aaatacataa aaaaagaagc ataggtatgc tatcaccctg cttcttctta
     3901 tgatttgtac gatcaattta ttctatcact ttactaatta atttgaaaat ctaataaaaa
     3961 ctctcactct aaaagagtga gagactgcaa attagagtac ggtctttttt ggagttccgt
     4021 tctttaatat taacaaatga ataaaatcct tacaacattt gagaataagt tatcagtaga
     4081 cacttacaaa aaattctcat actatttcct ccatcattag tttttgtcta tataaggtat
     4141 accctaacta gacacgaccg cgacattgaa aaatctggtt ttctcgtgta tgattagggt
     4201 gttttttaaa tagttgcaga aagtaggtaa aaattttgag agttgcatac atacgtgtct
     4261 catccatcga tcaaaatgaa caacggcaga ttgaagaaat gaaaaaattc ggtgcagaga
     4321 gaattttcat tgagaaacaa tctggcgcaa cgattactca tcgacctgtt tttcaagaag
     4381 cactggattt cgtccgagat caagatattt ttattgtgga agctattgat cgacttggaa
     4441 gaaattacga cgagatcatc gcttcagtca attacttgaa gaagaaaaat gtgcagctga
     4501 tcatcacaag tctaccgatc atggccgaag caattggcaa tcctctcctg gataaattca
     4561 ttaaagattt gatcattcaa attttggcca tgatcgctga acaagaacgt accgaatcta
     4621 aacgacgtca ggcacaaggc attaagattg caaaagcgaa cggtgtttat aaaggacgtc
     4681 ctaagctata cagtgccaat gcaaaagatc cccaacgtcg tcttgtttat aaaaacatcg
     4741 ttgaggatct aaaaaaaggt gttgcgatcg ctaagattgc aaaagactac aatgttacga
     4801 gacaaaccgt atatagaata aaaaaagaca gcatggtcaa tcacgaatag ttgatcatgc
     4861 tgctgtttct attttatttg cttacttttt gaaaaactgt tctaactttc atcaataact
     4921 cctcaactaa actatcatgc gctttttcca aaaaaataca ctctctggct tcataatcaa
     4981 ttgtcgttag ttgatctaat aatacttttc ctgttgtctt catttcatat tctgagttta
     5041 tatttacata tagcggatag tttctagttg tactagaaat tggagcaact gtaacaacgt
     5101 tactataatc cgcaataata tcatgactta aaacaatata aggtctttta cctgtctgtt
     5161 cgtgtccaga tcgaggtgct gtattcaata atagaatatc gccttgtcta ggttttctca
     5221 ccatttttca ttacctactg cttcacctaa attttgaatc gtcacatgat ttctttcatc
     5281 agtgtaattt tcaaataact gttcaattgt taattcattt tctatattag ccgcttcgat
     5341 aattagccgc ttctgaccgt ttacttcttc ttgtattatc ttcactttgt cttctgtatg
     5401 aattccaaga tattccataa tatactttgg caatctaagg ccattactat ttccccattt
     5461 ttttataggt aacatttcca tagtagacac ctctttctgt atatacatag tataacatac
     5521 ataagaaaaa acgtatatac ttaattatcc ttttctatta aattagatta acattccatg
     5581 aatccataaa ctatgttaac atttatatga aaatagtcga aaggaaatc
//
LOCUS       DACCWK010000124         5519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCWK010000124 DACCWK010000000
VERSION     DACCWK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.129"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IVW65_002418"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IVW65_002418"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7901268.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="IVW65_002419"
     CDS             2714..2938
                     /locus_tag="IVW65_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901269.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            3055..3552
                     /locus_tag="IVW65_002420"
     CDS             3055..3552
                     /locus_tag="IVW65_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7901270.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3639..4031
                     /locus_tag="IVW65_002421"
     CDS             3639..4031
                     /locus_tag="IVW65_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7901271.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            4015..>5519
                     /locus_tag="IVW65_002422"
     CDS             4015..>5519
                     /locus_tag="IVW65_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP7901272.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT         1912 a          943 c         1154 g         1510 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
     2761 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
     2821 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
     2881 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
     2941 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
     3001 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
     3061 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
     3121 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
     3181 tatgaggaat acgccattca tgattacgag ttacccttta cggttgacga atacacttct
     3241 attagcgaac tcaatcgcct atgggaaatg gtatcggagt tgccagaaga actacaatcg
     3301 gagctatctg ctctgctcac tcatttttca agtattgaag aactaagcga acatcaagaa
     3361 gatattatca ttcattccga ttgtgatgat atgtctgacg tggcacgcta ctacattgaa
     3421 gaaacgggtg ctttaggcga agtgccagcc agtcttcaaa actatattga ttatgaatcc
     3481 tatggtcggg atttagacct ttcgggaacg tttatctcaa ccaatcacgg gatttttgaa
     3541 atcgtctatt aaatctgtcg gtacattctt actggcagat tttcttgttt acggggtagt
     3601 caaaagacta tccccatttt tatgaaagga ttgattacat gaagaaaata cgaagctata
     3661 ccagtatctg gtctgttgag aaagtgttgt actccatcaa tgactttaga cttccgtttc
     3721 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtt atgatacttg
     3781 gcaacttgcc ccctctttct atgatagagg gagcatttct caaatacttt gggattcctg
     3841 tggctttcac atggtttatg tctacaaaaa cctttgatgg taaaaagcct tatggatttt
     3901 tgaagtctgt cattgcttat gcactgcgac gaaagctgac ctatgcagga aaaaaagtaa
     3961 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     4021 tatccaatta aatatattga aaacaatctg gtctggaata aagatgggga atgttacgct
     4081 tactatgagc ttgttcccta caattactca tttctaagtc cggaacagaa aatacaagta
     4141 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaagattca tgctttacaa
     4201 atcagtacag aatccagcat actttctgca caagaacgtt ccaaaaatga agtcactgga
     4261 aagctcaaaa cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     4321 attggcgaaa atcaagtgga ctaccgtttc tttatcggtt ttaagttgct tctcaacgat
     4381 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgcttttac tgattttgtc
     4441 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     4501 cgttttcaga agatggaaaa gcttttagaa aataaaatct cccgtcgttt caaaatccga
     4561 agattagata aggatgactt cggctatctg attgaacacc tttacggaca gacaggcact
     4621 gcctatgaag agtatgagta tcctctatca aagaaaaagc tggaacatga aacactgatt
     4681 aaaacctatg acctcattaa gcctactcgc tgtctggtgg aagaaaaaca gcgatatttg
     4741 aaaatccagc aggaagacga aaccgcctat gtagcttact ttaccatcaa cagtattgtc
     4801 ggcgaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttaccttt
     4861 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     4921 cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa
     4981 accagctcta atgtggcgga agctctggaa agtgtgaacg agctggaaac caatttagac
     5041 caaagcaagg aatctatgta taagctgtcc tatgttgtaa gggtatcagc aaatgacctt
     5101 gacgaactta aacgtcgttg taatgaagtg aaagatttct atgatgattt gagcgtgaaa
     5161 ctggtacgac cttttgggga tatgctgggc ttacatgaag aatttttacc tgccagcaaa
     5221 agatatatga atgactatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     5281 ggtgctactc aaatgctggg tgaaaatgag gggatttatg ttggctacag cttagatact
     5341 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     5401 aatgcgttag cgtctgcctt tgtcggttcg ctgggtggtg gtaaatcctt tgcgaataac
     5461 cttatcgtct attatgcagt gctttatggg gcacaagcag tgattgtaga cccaaaagc
//
LOCUS       DACCWK010000125         5465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCWK010000125 DACCWK010000000
VERSION     DACCWK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.130"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..448)
                     /locus_tag="IVW65_002423"
     CDS             complement(<1..448)
                     /locus_tag="IVW65_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7901273.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(755..1852)
                     /locus_tag="IVW65_002424"
     CDS             complement(755..1852)
                     /locus_tag="IVW65_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7901274.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1960..3987)
                     /locus_tag="IVW65_002425"
     CDS             complement(1960..3987)
                     /locus_tag="IVW65_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7901275.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4132..4863)
                     /locus_tag="IVW65_002426"
     CDS             complement(4132..4863)
                     /locus_tag="IVW65_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7901276.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            complement(5179..>5465)
                     /locus_tag="IVW65_002427"
     CDS             complement(5179..>5465)
                     /locus_tag="IVW65_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7901277.1"
                     /translation="NYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKR
                     YSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         1552 a         1083 c          873 g         1957 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aaccatccta
      721 caataataaa aatttactat ttgttatctt aaccttactt ttctttctct tctttttttc
      781 tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa gctactaaga
      841 tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat tccttggcta
      901 cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag ttgatcgtag
      961 tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa acagtcattg
     1021 taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga aaagaaaaat
     1081 ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact tcagaatgtc
     1141 ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt tgatcatttt
     1201 gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact tcatgaagag
     1261 taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata atataggtgt
     1321 attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt tccgtctgga
     1381 tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc ggcatcctga
     1441 catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt ggatagctca
     1501 cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt tttccgtatt
     1561 caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt ttcacttctt
     1621 tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga tcaagttgaa
     1681 gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc gtcactgaaa
     1741 aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa ataactagga
     1801 gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc atatgtaaga
     1861 gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac ctgtgccctt
     1921 cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg ggacatctgc
     1981 aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta caggaatatt
     2041 tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc tttcaccctt
     2101 ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat tttcagaggt
     2161 aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt tctcttgttt
     2221 caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc cacctgaaaa
     2281 ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat tgacatattg
     2341 aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat cctctgtatt
     2401 ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc cttcaatatc
     2461 aataggagtt tctttatact tctctcctat tttcccagtg attgttttca tttcagcaat
     2521 atcaatctga ctgtctttat ctttataata aacatggata tctatctcct tcagctttgc
     2581 gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa ggtattcaaa
     2641 atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca attcgattat
     2701 taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat ttccggttat
     2761 caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg gaatagttaa
     2821 ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaagaaaat ccccctcgac
     2881 cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga tggccccata
     2941 atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa gtcgcgtgac
     3001 agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc aatttacttc
     3061 tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagcgac cgacaccttc
     3121 caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca agcgccatgt
     3181 gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag aataattatt
     3241 attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat cagcagttat
     3301 ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact caatcgagag
     3361 cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc ccatacctgg
     3421 cgatccaata acttgactgg gattactagc cattctagga tcgccagtca atgtaaaggt
     3481 catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt taaatgtaaa
     3541 atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag acctttctgc
     3601 tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac cgataggtgt
     3661 ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa ccgcagaaat
     3721 tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt cagtggtctc
     3781 tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg attctattaa
     3841 actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat gctccaactg
     3901 attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga gactgcttaa
     3961 aatgagcacg atcctcctct ttttcaatat atcttttcct cctgatcgtt aaattcctag
     4021 atgaactatt cgtatccaaa caaatttagc ctagcctttg acaatttttg ctcgattacc
     4081 tcgcaagaca atatgactac gtatttattt ttaacagttc ttttcagtaa actagttgtt
     4141 aggtgtatct tctaatgtcc aaactaattt agtggaatat tgttgcgcta acttgacaga
     4201 tttaccgggt accgaaagct gtaccgcatc ttgattttca ttttcatttg caccaaatcg
     4261 atagacccaa gtccccacac ctgttcctga tttagcatta attgctaacg tattgtttcc
     4321 tcccggtgtc aacgttaatt cagaattcac cgtttgcggc gtatatgttg gatcaaccaa
     4381 agaaacagcc tgccctttcg taaatttcag ctgtgctcca actagttcat ccccactggc
     4441 agttttaaac tgttctggtt gagagacact taatttccac ccagataagg tacttctcct
     4501 atctgtcact tggacatatg tcggttttct agacccatcg ttcatcacat cagcagctgc
     4561 ataatacgtt ttatccgcag tagaaatttt ttgagtccca aatttgaatt ttgatccata
     4621 atcaatcgat aatgcccctc ctgttccagg gcttggagga tctgatggat cgacaggttt
     4681 attgggatca ggattctctg gatcaacagg aggagttaca ccctcatctc ctgcttcaaa
     4741 tgtaatcgtt ccttctgtgt tatattcctt tgggtatact tcagttgcag aaacaaaagt
     4801 cgtacttgct accatgctga ataacatcat tgctgctgtt aatacgattt cttttttcat
     4861 cattgacatt cctctttctt tatttactaa acatatcagt cgattggatt aatccatagc
     4921 aaaattctgt tttacgatac ttatgaaata aaagaacata gacctatatt tggtcctatg
     4981 tatcgattag ttataaatga ttatcagccc gatttgattt ctagttttta ttgcaacttg
     5041 atgcaaacac aatctagaaa tacagctgtc atagccttta aaataattcg taagtccccc
     5101 tcctatctat caaaatgtga cttgacgaga gtgtaaaata ttttgtgtaa ataaattgtc
     5161 ctcctgcaaa ataattagtt actcagtaaa cattgaaact aatgtatcgg ttacctgttg
     5221 aaaaccttta tggcttctgt ttagaaattt ttgattgtat gtatcaaaaa tgctgactag
     5281 aaagcgttct agtgattctt cattttgaaa ctgctctttt ctacggctgt atcttttaat
     5341 ttgcttattg aaagactcga ttagattggt tgagtaaatg gttctacgaa tgctaggtgg
     5401 aaaatcataa aaagttaata agtcttggtt ttctatgagt gactgcgtca ctttaggata
     5461 gtttt
//
LOCUS       DACCWK010000126         5215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCWK010000126 DACCWK010000000
VERSION     DACCWK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.131"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            55..930
                     /locus_tag="IVW65_002428"
     CDS             55..930
                     /locus_tag="IVW65_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7901278.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1034..1798
                     /locus_tag="IVW65_002429"
     CDS             1034..1798
                     /locus_tag="IVW65_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7901279.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1815..3227
                     /locus_tag="IVW65_002430"
     CDS             1815..3227
                     /locus_tag="IVW65_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7901280.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            3244..4569
                     /locus_tag="IVW65_002431"
     CDS             3244..4569
                     /locus_tag="IVW65_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7901281.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            4582..5040
                     /locus_tag="IVW65_002432"
     CDS             4582..5040
                     /locus_tag="IVW65_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7901282.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1767 a          756 c         1094 g         1598 t
ORIGIN      
        1 ttctttctta ggtcgttttt tgtctttaaa aattgtttgg agacaaaaat atgtatgaag
       61 gagtctgcaa gttgttttgt tttatcatat agaggaggtg aattaacggt gtatgtgatg
      121 aaaaagctga atcataatgc tgtattgatc gatgacgatg gaatagaaaa aattgcgatg
      181 gggaaaggga ttggcttttc tgctaaagtg agtaagccat ttgatgttga ggatgctgac
      241 aaattatttg tattagattc taaagaaaag atcaaacgat tcagtgagat agtctcgcag
      301 attccaatcg aatttatgga ttttgcggaa gaagtgattg aactgataac agctaggatc
      361 tcctctccat tagattcaaa tatctatatt acattgacag atcatattta ttttgctatt
      421 caacgaaaaa gagaagatag agatgtcaca gcaattatgt tgccagaaat gagattcctt
      481 tatccaaatg aatatgatat agcagtagaa gtggtcgagc gaatcaatca tcgatatcaa
      541 accgagttag ggtcaaatga agtaggattc atcactatgc atatcattaa tgctgagcta
      601 ggagagccta atagtttaaa gagcttaaaa atagtagaaa tgacgaccac gattttgtcc
      661 catctagaaa aatcctattc aaacgtttat gataaagaat cactgactta tcatcgctta
      721 atggttcata tcaaattcct ggttaaacgc ttgatttatc aggaagaaac gccagtagaa
      781 aatattggtt tttttactga aacatttaaa catagcaaag cgtattgtac agcactaaag
      841 attaaagata tcatcgaaca gcaatttcaa cttgccattc aagaaaatga aatcatctat
      901 ttggcaattc atctagcaag aatctattaa aataagggcg taactatcta taggcgcaac
      961 caaatgacac atagtaaatg gactatgatt atttggttgc gcctatttta gtagaaaaaa
     1021 gaaggagaat gaaatgggaa aagtgatttt taattcggat gattttggct atagccatgg
     1081 tgtgaattat gggattatgg atgcttatca gcgaggaatt ttgacatcaa ctacattgat
     1141 ggcaaatatg ccgggtttcg agcatgctgt gaaactaaga aaggaaatgc ctaggctagg
     1201 tgtaggagtc catttaacat tgacgtgtgg caaaccactc ctcaaaactg tcgatactct
     1261 tatcgaaagc ggagagttca gatccttatc ttattatcag catccattta acattgacaa
     1321 agatcaactg tatcaggaat ggaatgctca gattcaaaaa atttatcatg cggggatcat
     1381 accaactcat ttagacagtc atcatcatac tcatactttg gatgaaaatc aggaggtagt
     1441 tgttacatta gcaaaaaaat atgatttgcc ggttcggagc aactttgaac gaaaagatga
     1501 agttcgtcat gtcgattatt ttgagccttg tttcgatgtc gtaggagaaa ctgatgaaaa
     1561 aaaacgacaa atcaatacga gtgtggaaga atatttggca agtttactca tgatattgag
     1621 agaagataaa acgatcgaag tcatgtgtca cgcagcgtat ttggatcagg aattgttaaa
     1681 tggttcaagt tttgtgtatc ctcgtaccca ccaagtcgaa tttttaattc attcagcatt
     1741 tgctcaaaaa gtgaaaaatg atcctttgat tgagttagta acatatgagg atatttaata
     1801 gaggaggaat aaaaatgaat gttaaatcaa atgtgcagaa gcttggaaga gcgatgctac
     1861 ttccagttgc agcgatgcca ttagcgggat tgatcatgcg tttatcagct gacgatatgc
     1921 tgaacatccc agtaattggt gcagctggaa atgccgtttt tggaaacttg gatatcttat
     1981 ttgctatagg agttgcaatc ggttttgtca aaaataagga caaaggaata cctgcactta
     2041 ctggtttttt agcgattgct accttgaaaa aaggcttaga aattatggac ccagaagtga
     2101 atatggggat ttttggtgga attatttccg gtttagtagc ggcatggacg tataatcgct
     2161 ttaaagaaca aaaattacca gtagtttttt cttattttgc gggcgaaaaa ttcccattga
     2221 ctatggtgat gattctacaa acaatcgcag ctgttttttt gggctttatc tggccgctca
     2281 tccaaattgg gattgataat tttgcaaaac tgctcgttga tatgggagca ttaggtgtgg
     2341 ggatatttat gtttttgaat cgtttgctga tcccctttgg actgcaccat gtgttgaata
     2401 cgtatattta ttacgattta ggaagctata catctcctag caaggaagtt ttcagaggag
     2461 agatgacacg ctttatcaac ggagatcctc aggctggact gtttttatca ggtttttttg
     2521 tagtaatgat gtttggtata ccagctatat gtctagctat ctataaagca gcttataaag
     2581 aaaataaaga gatggtcaaa ggaatcatgg gaagcggtgc aggaacttca tttattgcaa
     2641 atatcactga gcctgttgaa tttagtttca tgtttctttc tcctatgttg tacgtgatcc
     2701 atgcactttt tgccggttta gcagggattg tctgttactt attcaatata cgaattggtt
     2761 ttacattcgg agcatgtatt gttgattatc tcatcaattt tcgaatcgct accaatgcta
     2821 ttttgattct tccaatcgga attttctttt ttgccttgta ttacgttact ttttattatt
     2881 tgatcaataa gagaaacatt cagacattag gacgtgaagc taaggcagag tttggcaatg
     2941 aagtaacctt agaagaaaca gagttagggt tagcaagtaa aaattatgaa tatatggcaa
     3001 caaaaatgct acaagcgttt ggaggaaaag ccaatatcct ggatgtctat agttgtaata
     3061 cacgcctacg ggtagaggta gtggatccca ccatggtcga ggaacagcga atcaagcaat
     3121 tagggataag cgggatcatt aaaccaactg aaaaaaatta tcaaattatc atcggcttag
     3181 aagtgactta tgttatggca gaattcaata aattattaga agaatagaaa ggaaaagatg
     3241 atgatgaaaa aacagattat cactatagct ggaggcggga gtacgtatac accaggaatc
     3301 gttcaagcca ttttgaacaa tgaaaaacgg ttgccattat ctgaaatacg tttatatgat
     3361 attgatgaag aaagaaatat ggatatgtac ttgattgtta aatttatgct gaaaagaaaa
     3421 ggtttttcaa atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc
     3481 gattttgttt tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag
     3541 attccactta aacatggatt agtaggacaa gaaacgtgtg gactcggagg atttgcttat
     3601 gggatgcgct caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc
     3661 aaagcatgga ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt
     3721 aaatatccga atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg
     3781 attgcagtga attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac
     3841 cactttggct ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag
     3901 attattgaaa aactaataac aaaagaaatg caagtcgccg attttaatat aggagataaa
     3961 acttggcaaa aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc
     4021 ccgaataatt acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa
     4081 gagtatacac gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct
     4141 gataaaatac gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga
     4201 caatatatcg tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta
     4261 atcgttccta atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg
     4321 gcatatgttg gtgcaactgg tgtggagcca attacactaa gaaaaccaat cagtgatttt
     4381 cataaaggat tgatggaggc acaagttgca gtagagaagt tactagtaga tgcttacttt
     4441 gaaggttctt atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc
     4501 agagtcgcaa aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca
     4561 ttaaagtaga ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta
     4621 gctagtggtc aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg
     4681 ataggagttg gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga
     4741 ataatcgata atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga
     4801 aaaatactga tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata
     4861 cgggttgaaa gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag
     4921 aagttggagc aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa
     4981 ggaaatttat taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag
     5041 tgtacgatga aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg
     5101 aaatgatatg tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt
     5161 tttgttgctc ctatgattta gaaaacttat tttcaggatt cttaaaaata aattt
//
LOCUS       DACCWK010000127         5056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCWK010000127 DACCWK010000000
VERSION     DACCWK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.132"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            5..2377
                     /locus_tag="IVW65_002433"
     CDS             5..2377
                     /locus_tag="IVW65_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901283.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="IVW65_002434"
     CDS             2438..3898
                     /locus_tag="IVW65_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7901284.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..>5056
                     /locus_tag="IVW65_002435"
     CDS             3909..>5056
                     /locus_tag="IVW65_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7901285.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKEVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEIPTPIHTPNSRYVNDKKVQEHYAIIPTKT"
BASE COUNT         1916 a          851 c         1091 g         1198 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaatga tcaaatcgaa aagatgtttg aacaccaaag
      121 aaagaccaac ggtcaaccaa tgaacgataa gagaaacggg cattcttggt ttaggcaaca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggttataaca agtacggtgg
      301 cctggatatg acgatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ctgccacgat ccgaaaatac caacgaaaat tggaaacatt
      421 agaacagctg aaagaaaaat cagagaaagg aaaagaaaat attcttcctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaagaat ccaactatct attttctgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagtcgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccagc cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aaaaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatt ggtgatccta aacagttaaa
     3121 attttttgag agtagtattg atttacttag ctacgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagttga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aagagaatac aaagtcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagtc aatgcgtctt ttggtcagca gctttctgtt aacgattcaa tagaagtcat
     3601 tagtttgaaa gatatttgta gagaagtcgc aaaagaatta aaagattgtg aacgctcgaa
     3661 tggtacgtat gattttgatc gtttttatca aggaaaaggg gatattaatt ctcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagtg aaaaaggatt ggaacgatca attgaagcat gagatccatc aacaggaaat
     3841 tagaaagcaa aaacgtgcga tgttgtttca acaaggcaga caacaagaaa gggagtgaga
     3901 tcgtcacgat gaaaactgtc attttagcag aaaaaccgtc acaagcgaag gcctatgccg
     3961 atagcttttc taaagccacc cgaaaagacg ggtattttga aatccaagat cgattgtttt
     4021 cgggtgaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagagcattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa cgtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagca ggcgctgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca attacgagac gcagaagaga
     4381 cctatcccaa atttgaagaa gcacaagcac gccagatcgc tgattggttg atcggcatga
     4441 acggcagccc actgtatagt ttactcttgc agcaaaaggg cataccagga agtttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca gctgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aagccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa ggaggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc tagccttcag tctaaaatga
     4801 atcaactcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttatat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa attccaaccc
     4981 ctattcatac gccgaatagt cggtatgtga acgataagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaac
//
LOCUS       DACCWK010000128         5053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCWK010000128 DACCWK010000000
VERSION     DACCWK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.133"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..612
                     /locus_tag="IVW65_002436"
     CDS             1..612
                     /locus_tag="IVW65_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7901286.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            685..1614
                     /locus_tag="IVW65_002437"
     CDS             685..1614
                     /locus_tag="IVW65_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7901287.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1772..2509
                     /locus_tag="IVW65_002438"
     CDS             1772..2509
                     /locus_tag="IVW65_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP7901288.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2557..3735)
                     /locus_tag="IVW65_002439"
     CDS             complement(2557..3735)
                     /locus_tag="IVW65_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7901289.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3758..3841
                     /locus_tag="IVW65_002440"
                     /pseudo
     CDS             3758..3841
                     /locus_tag="IVW65_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3962..4363
                     /locus_tag="IVW65_002441"
     CDS             3962..4363
                     /locus_tag="IVW65_002441"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901290.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4570..>5053)
                     /locus_tag="IVW65_002442"
     CDS             complement(4570..>5053)
                     /locus_tag="IVW65_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7901291.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1581 a          868 c          980 g         1624 t
ORIGIN      
        1 atgtatttta tactacctac actttatagc cttttatgtg taatggcacc atgtattatt
       61 tatatggtat ttttttgcaa aaaaaatacc ctgaaacaca atatcctgat tttgattttt
      121 atgctgtact gttggaaagt ttttgaattg actggtacag gtggactaac agatattttt
      181 tatagacctg atacaggtag gaaattttct ctagtacagg gcagtgttaa tcttgtacca
      241 ttcagtggac taaacattag ctttatatta aatatagtaa tgtgtgtgcc cttaggattc
      301 ttactaccaa tcatatggaa aagttatagg aaaatagttc ctgttttctt ggctggggct
      361 ctattttcac taattattga agtctcacaa attataacta caagagcaac agatattgat
      421 gatttaatag caaatacctt tggtgcggtt ataggcttta tactgtgggc tgttttaagt
      481 agagtggtgt ccttgaagga caagtcatct acagtagatt atttagaacc ttatatttat
      541 atattgattg gttttaccgg aaaattctta ctgtattttc ctttctggtt tgcttttgaa
      601 gtattatcct gataattcca tagaaaataa tgagtttgag ttgagaaaga atattcttgt
      661 acacaactct ggaaaggagg aagtatgaaa agattcttaa aatgggggct tgttctttta
      721 gcgtctttga ttgtgattct aatgtgtaca gcggttgtta tttcttatgt gaatcggccg
      781 tatgcaagag ctgtttatga taggattaaa cgcattcttg acaaagattt ttatgaccat
      841 ctatatctag gttcttcatt tgaaataata aaccttgata caatggatat tcatagctat
      901 atgaaaaaag accaagaaga attacccgcc tcattgagca aattatttac gattgattat
      961 gcgcttagtg tcctaagttt agatgaacag attacaatta gtcctactgc acttgacatg
     1021 cagaaagctg actcatctac tgcacatctt caagaaggag atattttatc tgtttatgac
     1081 attatctgtg ctatggtctt accctcagga aatgatgcag cttatagttt ggcaatttat
     1141 acaggtggaa aacttgatcc tgatgcaaag aatgatgaag aacgctatca aagcttcatg
     1201 aaaaacttag atcgttatct aagtgagcaa ggctataaac atactcattt tactgacgct
     1261 gctggctatg actccaatgc tacaacatca gcggaagatt tgacaaaagt agctgcaaag
     1321 ctactaaaga atgataaatt tgcagaaata gttgccaagg aaacttatag agcaaaatca
     1381 attaagggaa atgtttataa ctggaatact acgaatcaat ttatgcaaca agataataga
     1441 tactttactg aaggtgtaaa aggtgtaaaa actggatcgt ttgatgggag atacaatcta
     1501 tgtgtgtatt atacatctag ttctgataac cactatttac ttgtaagttt tggttcagag
     1561 agtgatagta ggcgttatgt agatgttcaa aatctattgc ataatctgcc ttgaaatttc
     1621 gtgcctttaa gcgcgtatct aaagtttctt tgaaaagttt gagaatgaag aaagtgagca
     1681 gtcgaaaaac ggttgctcac ttttttgccc taaaatcgaa aaaatataaa aaagtccttt
     1741 acaacacgtc tcaggaaaaa ctgctaagtc aattctaata tcttgtggag caagacttgc
     1801 accttttgat ataagagagc ttagagaact aatgagcgaa gatgaactag aactcgataa
     1861 aattggagat agaaaaactg ccttgtttgt aataatatca gatacagatg atacatttaa
     1921 ttttgtagtt tcaataatgt attctcaatt atttaatcta ctatgtgata aggcagatga
     1981 tgtgtatggt ggaagacttc cagttcatgt taggtgtcta cttgatgagt ttgcaaatat
     2041 cggtttgatt cctaaatttg aaaaactaat agcgacaatt cgttcaagag aaatatcggc
     2101 aagtataata ctgcaagcac aatctcaatt aaaagcaatt tataaagacc atgcagatac
     2161 aatagttggt aactgtgact ctacactttt tctaggagga aaagaaaaaa caacagtaaa
     2221 agaattatca gaaaccttag gaaaagaaac catagaccta tataacacat cagaaacaag
     2281 atcaaatcaa aaatcttttg gtctaaatta tcaaaagact ggcaaggaac ttatgagtca
     2341 agatgaaata actgtaatgg atggtggtaa gtgtatttac cagctaagag gagcaagacc
     2401 ttttctatct gataaatttg atattacaaa gcataagaat tacaaattgt tggaagatta
     2461 tgataagaaa aacttgtttg atgtagaaga gtatttaaaa caaagatagc gtaaaatatt
     2521 ttgtgtaaat aactcgtcct tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa
     2581 tgtatccgtc acttgttgga agcctttatg gcttctattt aagaattttt gattgtatgt
     2641 atcaaagatg gatactagga aacgctctag tgattcttca ttttgaaact gctcttttct
     2701 gtggctgtat ttcttgattt gcttattaaa ggattcaatc aagttcgtag agtaaatagt
     2761 cttgcgtatg gctaatggaa aatcatagaa agtgagcagg taatcgttcg aaaggatcga
     2821 ttccaccatt tttggataac tggttttcca tttttccgca aaagcaccac gtgcttccaa
     2881 tgccgccttt ttagatgacg cttgatacac cattttaaaa tcatcacaga cttccttacg
     2941 atcatcgaca cgcactttgt gactgatatt acgggaaacg tgcacacagc aatgttgaaa
     3001 acgggctttg gggtagaacc gctgcacggc ccctgccatc ccctttaagc catccgtgat
     3061 gaaaaggagg acgtttttca cgccacgttc ctggaggtct attaaaatct cttcccaaat
     3121 cgtgatagat tcagttggtg caatcgcata gctcaacact tcctttgaac cgtccgggcg
     3181 aatgccaact gcgatatgaa tggcttcctt ggcgacggtt ttccgcttta aagggatata
     3241 ggttgcgtcc atataaatag cggcataacg gtcatgaagc tcacgttcct taaatgaagt
     3301 gacttcttcc gtaaatgatt tagtcatatt ggacatggtt tggggcgtgt agtgatgccc
     3361 atacattttc tcaatcaagt ctgcgatttc cgacatggta ataccttttc ggaagaggtg
     3421 aatgacggtc tcctctaagg tatcattcgt ccgtctataa gcaggaacag tctgttgctt
     3481 gaactcgccg ttgcggtcgc gcggaatctg gagatgaagt tccccgtact cggtcttgac
     3541 cgtacggtca taggagccgt tacgagaatt acccgtgtta aaaccaatgc gatcgtactt
     3601 ttcgtaatcg aggaaagccg ttaactccgt tttgagaagc gtattgacag ctttttccaa
     3661 atggacacgg aataattcat tcaaatcgcc tttattcgct agagtcttta gaatttctgt
     3721 agtaaaatcg ttcatatgga agtcctcttt tctgtgaatg tgttgtggta actctattct
     3781 actgaaggga cttccttttt tctatttaca caaaatattt tacactctcg gattgaaaga
     3841 acgcggtctt tcaggagtaa agatggtcat ctcggatgcg catatagggc tcacaaaagc
     3901 tgtgaaagaa taccttcttt gtgcgagcta gcagagatgc caagcacatc tccttcgcaa
     3961 catttccgac aaattctcca agaaaatctc aagcgatatc aaagaagact ttaaagccat
     4021 cttcaaaacg gcaagcatcg agctggctgg agaacgtaaa caacttatta tggataaata
     4081 tggggaagac aagaaagtcg caactgcctg taaaatattg gatgagggct tcgaagatgc
     4141 catgcaagta atggttttac cagaaaatat ccgtcaacga ttgagaacaa cgaatgttct
     4201 tgaaagacta aatgaagaag tacgccgtcg tgaacgtgtt gtccgaattt tcccaaattg
     4261 tgattctgtt attcgtatca tcggtgcttt attgatggaa aaggatgacg aatggacttc
     4321 tgcacctaga aaatatcttg aatttgatcg taacgagatt tagcacatta agccgccaat
     4381 agtataatat actggtgaga agtatcaaga gacgcttcgc tttttgctct tgacacttct
     4441 taccagcata ttggaccgta gctaggctca acaaggcaaa acatcgtact ttgagatttc
     4501 ctttttacac aagattttgg acttgacaca gcccctatgg ttcttacctt gtttatgctt
     4561 atttaaatct tactttatta cagtactttc aaacttcgtt ttgtaagccg ggtattttga
     4621 tatttcataa ttatgtccct ctttttgaat atattctttg atatctgctt gttcatctac
     4681 actttctttg cgtaaaatac gttcgatcat tgagcagcag tgcatgtaaa gcaagaacaa
     4741 ttgattgttt gaaaattcga tccccattag tttttcgagt tctttgacgg ccttttgtac
     4801 caatctcaaa accttcattg catcaaggat cgttaaattt tctacgatct tgttaatcga
     4861 gaaattaaag tattgagcac aattataggc gataaatgga gcatttgatt ctatcacctg
     4921 ttgttccttg gcgtattcat aaattttttc tgccaaaaag gtttttcctg aaccgcttac
     4981 acccatcaat aaaatcggta atccgttatg aggatacaag acagccgtct tgatttgatc
     5041 gatcgcttcc ttt
//
LOCUS       DACCWK010000129         5006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCWK010000129 DACCWK010000000
VERSION     DACCWK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.134"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..182)
                     /locus_tag="IVW65_002443"
     CDS             complement(<1..182)
                     /locus_tag="IVW65_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7901292.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IVW65_002444"
     CDS             517..951
                     /locus_tag="IVW65_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7901293.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IVW65_002445"
     CDS             974..1294
                     /locus_tag="IVW65_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901294.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IVW65_002446"
     CDS             1435..1626
                     /locus_tag="IVW65_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901295.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IVW65_002447"
     CDS             complement(1697..1954)
                     /locus_tag="IVW65_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7901296.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IVW65_002448"
     CDS             2112..2375
                     /locus_tag="IVW65_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901297.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IVW65_002449"
     CDS             2399..2587
                     /locus_tag="IVW65_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901298.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IVW65_002450"
     CDS             complement(2670..2855)
                     /locus_tag="IVW65_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901299.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IVW65_002451"
     CDS             3070..3441
                     /locus_tag="IVW65_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7901300.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IVW65_002452"
     tRNA            complement(3591..3663)
                     /locus_tag="IVW65_002452"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IVW65_002453"
     CDS             complement(3709..4137)
                     /locus_tag="IVW65_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901301.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IVW65_002454"
     CDS             complement(4197..4955)
                     /locus_tag="IVW65_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7901302.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1743 a          784 c          978 g         1501 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt attttt
//
LOCUS       DACCWK010000130         4957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCWK010000130 DACCWK010000000
VERSION     DACCWK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.135"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..1155)
                     /locus_tag="IVW65_002455"
     CDS             complement(1..1155)
                     /locus_tag="IVW65_002455"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7901303.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="IVW65_002456"
     CDS             1340..3781
                     /locus_tag="IVW65_002456"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7901304.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4111..4815)
                     /locus_tag="IVW65_002457"
     CDS             complement(4111..4815)
                     /locus_tag="IVW65_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901305.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1553 a          915 c         1039 g         1450 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagccgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt tctctatcag aaaatatgtt tattattaga attttttata gttaaggtta
     3901 ggcatcttaa gaaatcaggc aactccaaga tatcttcagg tagaagagaa gtcgcctaac
     3961 attgtaggat cataggtgat ttgttggaca ggtcaagctg atatttgagc ctgatgttag
     4021 aaggaatttc tttttacttg ctgtagaaat tattatatga ctaaaaaggc tgggatttta
     4081 aatcccagcc ttttatgaca aatagaattt ttatcgatca gtaatcgctg tcgctgcaaa
     4141 ttgttcgatc aaaggaatca aagtcaattt gactttgtat ttccgttcga gtgctttttc
     4201 gataacgaac tcattttcag tagtcataga aatagttgct aatttacgag gttcatcagc
     4261 atttttttca ttaataacta cttcatgttc attttgttct gtgtaaaatt taggatactc
     4321 agcaacggct tttgaaaaat cagcaaaact taaagcggca ttcgtatcag ttgagcgtgt
     4381 attttcagtt gtgtccgctt ttggtttttc cgttttttct tctggcacac tgactgtttc
     4441 ttctgtaact ggtgtcgcaa cagcttgcgc agttggttca tcgtttttca atcgttgatc
     4501 attccagtaa gacacgattt caataagaag attgtcacgg aatcgttcga aaatcccgtt
     4561 ttctcctgaa cgaattctct ttaatacagt attgaagttg tcatcgattg gcacgttgat
     4621 ccgtgtatct tcatttgttt tcccagctaa tccgatcgtt aagatttgac ggtttttggg
     4681 atcaagcatg acttgtaacg gcaaaccata acgtcgcaag ctctcttctt ctagagaacg
     4741 gaataattgt cctcttacac catccaatga aggacggaat tgatttaata atttttgttt
     4801 ctgttttttc ttcatatgtt ctctccttcg ctcataccct ttcgattata agaaaaaaac
     4861 agccaaatga aaaggagtaa tgcaaaaaaa cttgcgtata cgcaaattac aacagaaaaa
     4921 tttgcgtatt ttcccgtttt ttgaaagaaa aataaaa
//
LOCUS       DACCWK010000131         4893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCWK010000131 DACCWK010000000
VERSION     DACCWK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.136"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            237..1877
                     /locus_tag="IVW65_002458"
     CDS             237..1877
                     /locus_tag="IVW65_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7901306.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IVW65_002459"
     CDS             2031..3353
                     /locus_tag="IVW65_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7901307.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IVW65_002460"
     CDS             complement(3415..3846)
                     /locus_tag="IVW65_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7901308.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IVW65_002461"
     CDS             complement(3910..4746)
                     /locus_tag="IVW65_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7901309.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
BASE COUNT         1510 a          970 c          908 g         1505 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact ttt
//
LOCUS       DACCWK010000132         4871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCWK010000132 DACCWK010000000
VERSION     DACCWK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.137"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="IVW65_002462"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="IVW65_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP7901310.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="IVW65_002463"
     CDS             724..1878
                     /locus_tag="IVW65_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7901311.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="IVW65_002464"
     CDS             2093..3061
                     /locus_tag="IVW65_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP7901312.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="IVW65_002465"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="IVW65_002465"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP7901313.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="IVW65_002466"
     CDS             4091..4699
                     /locus_tag="IVW65_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP7901314.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4779..>4871)
                     /locus_tag="IVW65_002467"
     CDS             complement(4779..>4871)
                     /locus_tag="IVW65_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7901315.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1501 a          936 c         1135 g         1299 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat
     4801 accttgattt cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta
     4861 tataatcctc g
//
LOCUS       DACCWK010000133         4864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCWK010000133 DACCWK010000000
VERSION     DACCWK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.138"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(108..1256)
                     /gene="nagA"
                     /locus_tag="IVW65_002468"
     CDS             complement(108..1256)
                     /gene="nagA"
                     /locus_tag="IVW65_002468"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7901316.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1332..2168)
                     /locus_tag="IVW65_002469"
     CDS             complement(1332..2168)
                     /locus_tag="IVW65_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7901317.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2352..3569)
                     /locus_tag="IVW65_002470"
     CDS             complement(2352..3569)
                     /locus_tag="IVW65_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7901318.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3952..4035
                     /locus_tag="IVW65_002471"
     tRNA            3952..4035
                     /locus_tag="IVW65_002471"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3986..3988,aa:Leu,seq:gag)
     gene            complement(4275..>4864)
                     /locus_tag="IVW65_002472"
     CDS             complement(4275..>4864)
                     /locus_tag="IVW65_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901319.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1490 a         1006 c          781 g         1587 t
ORIGIN      
        1 aattaaagaa acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaaacct
       61 taaaaccaaa cttaagaagt ttagttttaa ggttaactta aattatatta tgcgtggaaa
      121 cgttccacgc catctagata agttgcttcc aatgtcatat cagggttcaa tacgataaag
      181 tctgcatcgc gtccttgagc gatttttcca catgtatcat cgattttaca gctgattgct
      241 ggaatcagac tagccatcat gactgcttgt tctggtgttg ctaatcccca gtctacaaca
      301 tttttaattg cttctttcaa tttcaatata cttccagcta agttgccaga ttctagacgt
      361 gctgttcctt cttttactac tactggaaat tcgcctaaat tgtaatttcc atcaggcatc
      421 ccaccggcca tcatacagtc tgtaattaat gcgacatgat catgtcctgc tttttccatt
      481 aggattcccg cagcatttgg atgtacatga tggccatcac aaatcaattc agaaaagaca
      541 tgttctaaag ataataacgc acccaccata cctggttcgc gatgattcaa tccacgcata
      601 ccattgaaag catggacaaa tacacttgcg cctgcttcaa cagctttttg cgcttcttct
      661 agtgtagcat tactgtgtcc tagagaaaca actactcctt cttcagtcac ttgtttgaca
      721 aactcttcta caccttgacg ttccggtgct aaagcaattt ttttgattaa accacctgaa
      781 gcttcttgcc attcatggaa agtatccaaa tcaggatccc cgaaataact tgggttttgt
      841 gcccctttgt gttcttctgt gaagaaagga ccttcaaaat agattccttt gattttagct
      901 cctggtgctt cctgatacat tttgccgatt gtctcagcta catcttttaa acgttctttg
      961 cttgacgtca atgttgttgg taaaaatgaa gtcactccac aagataacag accttcagac
     1021 atcactttga tgccttctgc atcattatcc attacatcat gattcatata gccgtgaata
     1081 tgtgtatcta ccaaaccagg agcaatccat ttgccgcttt ggtcaatgat gttgacttct
     1141 ccctcaggta gttctttggt gtattcacca aaaagtccat cggtaatttc aagatagcct
     1201 gcatgtttga cacctgacgc taaaaagaat ttgtcagcat agataaaagt tctcattcta
     1261 ttctctccct ttgtttttct acctctaaag tactcttatt tagagaagaa gtcaagaatg
     1321 gtctacacca tttacaaatt ttttgaaatt tgtttcacag cttgtgctaa gccttcatag
     1381 aatgctgctc tttctggttc ttctttacgc ttgtatatta agcttttaag cacaaataaa
     1441 tcattgaggt aatagtagct attcttctct tgagcccaat agattccttg attcacctgt
     1501 tctaacgctt cgtcgatttc attttgatgg aataagaaat ttccataatt ataaaaaatt
     1561 tgcgaaaatt cactttttga tcgttcatca gctgattgat gcagcagttg gatgcttcga
     1621 ctcaaatagt agcgtgcttc agccgttttt cctgccacac catatatttt tccgatacaa
     1681 ctaatcagtt gtatttctgt gtcagaaaca tggattcttt ctttttggct agagtagctt
     1741 aatgcttctt tcaaataata aagggactga gagatatttt gacgcaacgt gaattcgcaa
     1801 acaccgagat aataataata gcgttgaaag tcagttgctt gatacagatt ttcagaaata
     1861 tcagaagatt ttagtacttg ttctaacttc cgataatcac gtttttggaa acaactatcc
     1921 aacagttcaa ataatttatc gtttgtccga gtagtttttc gagagagatt catcacatga
     1981 tcaatcgtta cccctagccg ttgacaaatc tgcatcatca ccaatacatt cggcagttcc
     2041 tcattatttt cgatccgact taatacactt tgtgaacaaa tatcatgcga aagcattttt
     2101 tgcgtcattt tttgttcttt tcgaatctct ttaatgatcg ttccaaagga ctcgattaac
     2161 gtttccataa gtatcctccc caaaatatgc gtattcgcat tctattcgta ttttacttta
     2221 ggaagtcgca aacttcaaga aaagacttgc aactaaataa tatactttag ttatttaaga
     2281 aaactccact agaaagagag cctcagtgtg tttgtctgtg gccctcctac tgaaaaattt
     2341 ggtttttaaa atcatttttg gcgataggtt gctaggaatt ctctcatttt cttagctgtt
     2401 tgtttcaaat catccatttt aggaagataa acgattcgaa aatgatctgg ctggttccaa
     2461 ttaaatccac caccatgtac aagtagaata tgatgttcat gtaaaaagtc caaaacgaat
     2521 ttttcatcat ccaaaatatt gaatcgcttt gtatcaattt ttgggaatat ataaaaagca
     2581 gcttttggtt ttacggctga aagaccagga atatcattga ttgcattata gatatattct
     2641 ctttgttcat agatccttcc gcctggcagg agcagctcat cgacactttg ataaccgcct
     2701 agcgcggttt ggataatttg ctgagacaat acattggaac acaaacgcat ggaggacaac
     2761 atattcagcc cttctatata gcctttgacg tgtgacttat ctccacttag gaccatccag
     2821 cctactcgaa atcctgctac acgatgagat ttggatagac cgttcagcgt aacaacgaac
     2881 aaatcaggcg ctaatgtagc aatcggaata tgttctagac catccatcac taagcgatca
     2941 tagatttcgt cggaataaat aatcaggtta ttttgtcgag ctacttcaac gatttcttct
     3001 aataattctt ttggataaag tgccccagta ggattattag gattaatgat aacaatagct
     3061 tttgtccgac tcgttatttt tgatttgata tcatcaatat caggatacca ttctgcctct
     3121 tcatcgcaaa tataatgaac aggtgtgcct ccagctaatg caatagaagc cgtccacaac
     3181 ggatagtccg gcattggaac cagtacttca tctccattgt ccagcaaccc ctgcatacac
     3241 atcgtgatca attcgctcac tccgttgccg gtgtagatat cattgatcgt cacattcgga
     3301 aaatgtttca gttggcaata ttgctcaatt gcttttctag cagaaaagat ccccttggaa
     3361 tctgagtagc cttctgagtc acgggcattc atgatcaaat cacgaatgac ttcgtttggt
     3421 gcttcaaatc caaaaggtgc gggatttccc gtgttcaatt ttaaaatact aatgccctct
     3481 tcttgcatcc gctccgcttc ttctagaacg ggaccgcgaa catcgtaact gacaccttct
     3541 aatttgttgg atttttcaaa ttctctcatc acttagccaa tccctcattt caaaaagtta
     3601 gaactttcca aattttttgt tatttactat ttcaatatca tactccatcc tagaaaattt
     3661 tgccacttat ttaggaaaaa cgtaagtatt aaaaaaacac aaacaagcgt tgagaactta
     3721 attgtcatga tagtcattcc atagattcgc tgcccaatcc attgcaattc cacaaatgat
     3781 tcctagcata aaaccaagca aaaaacgtcc gaatgcaagt ccaatcaata cacctaagat
     3841 cattcccagt cctgaaaatt ctccgtcata tttctccatc ttaataagtc ctcccatttt
     3901 tgagcttgac aactatgtaa aacctcgcta tcattagtgt gttattatta cgcagatgtg
     3961 ttggaattgg cagacaagca tgattgaggg tcatgtgagc gttagctcgt gtgggttcga
     4021 gtcccatcat ctgcactaca actcgtgaag ttaattttgc ttcacgagtt tttttgtttg
     4081 aacgtttctt tatgccatta tatacatatc actgtatcct ttacaaaaag aacgtacaat
     4141 catgtaaaca gcctgaggtg agtatatgaa ggtcggaaac aagctactcg tagaaacttg
     4201 caagtgttaa aacaattaat cgaaaaacga gtaaaatccc tcacctaata ctaaagcgag
     4261 ggattttttg tatattatct gtttatcacc gttattataa aggtacaaat accaagcacg
     4321 gttaatccaa attgcataaa ttcatttttt tgtacactca tgcttttcat tagctcttta
     4381 actgccatta gtactaagat ggcgataaca acataaagta tatagtacgt ccgcattccg
     4441 gaagcccata ctgtgggaga aaatcccata attaaccttg aacaaaatcc cattatcatc
     4501 aaaataacta aaaaaataga agttttctta ttgtcaaaac ttaaataaat tccaacaatt
     4561 aaacatagta ataatgccaa gactactaaa gtagctatcc aagtacctgg ataaaaaagt
     4621 gacagtttcg ttcctaactc tgtaaagaga ttattcaggt tactactact ccaaatcatt
     4681 gcccttttat ttccattgac gtaagtaaag ctttgaccca ttgtattacc aaaataaata
     4741 atcaggttta agaaaaacgg taatgcagtc aatatacgca cataatagtt ttgacatttc
     4801 ctatatgtta gaaggaaaat taagaaaaat agcaataaaa ataagatatt catgtctaaa
     4861 aaaa
//
LOCUS       DACCWK010000134         4786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCWK010000134 DACCWK010000000
VERSION     DACCWK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.140"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..813
                     /locus_tag="IVW65_002473"
     CDS             <1..813
                     /locus_tag="IVW65_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7901320.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IVW65_002474"
     CDS             807..1229
                     /locus_tag="IVW65_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901321.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2401..3369
                     /locus_tag="IVW65_002475"
     CDS             2401..3369
                     /locus_tag="IVW65_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7901322.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            3379..3849
                     /locus_tag="IVW65_002476"
     CDS             3379..3849
                     /locus_tag="IVW65_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7901323.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            4445..>4786
                     /locus_tag="IVW65_002477"
     CDS             4445..>4786
                     /locus_tag="IVW65_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7901324.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         1824 a          778 c          790 g         1394 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcgattat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agttttgtag agagtgacct tataggcact ctcacattac gaacgatacg
     1501 tccctcggac gtactgaaag ggtgggactt atgagcgaac taatgcaaaa tttggctagc
     1561 aaaaaagcga aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aaacagttta tttcagcttt gtcatggcgt gtgccgactt
     1681 ttttaaaagt ctaggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaagg
     1741 cgtttcaaat gtctaagtat ataaatatat aagaatagag ttaagagtgc ttaaatcgaa
     1801 ttttagaggg aattcagaca attttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acagttttag caaaggagcc ggaggcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcaactata gggagtaaga
     2041 ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctgta tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacaga
     2341 cattttgtat ttcataacgt ctgtatctct tgtgcgagta agaaaggagc tattttaaat
     2401 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     2461 acaccagtcg aaatgaattt gttcttctct atcgtatcaa gaatgcgtga tgtaggtgat
     2521 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     2581 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     2641 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     2701 atcgacggaa aagctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     2761 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     2821 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     2881 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     2941 acgatcgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     3001 ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc ctgtcattgg ctaccaattc
     3061 acatttaaac cagaaatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     3121 gaacaaaatc gggctttaga actagctaat cgacaaactc aacacgaaaa agaaactact
     3181 tcattacaag aaagaattaa ggaacaaaag cgaaaattag aacttttaca acagttagaa
     3241 gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg ataaacaaaa gttagacaac
     3301 aattccgata ttcctctaga actaagaaaa gacttacttg ataatcttca aaacttattt
     3361 aaagactaag gagtattcat gacagataaa tcaataaaag aatttgcaga agaactaggt
     3421 gtcagcaaac aagccattag aaagtatttt gataaactgc caaccaagtt gacacctaag
     3481 ttgataggtg gaaaatacgt aatatcagca aaagctcaag agtttataaa aaatagtatc
     3541 aaccaaataa atgaagttga tactgaagtt gacagtttag ttgatagtat tttaattgca
     3601 gaattaaaag aaaaaaacaa acaaattgat catttacaag gggtcataga aatacaacaa
     3661 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacaaat cgacaaacta
     3721 caaaaacggt tacaattagt ttatacggaa gaaacttcag aaaatgaatc aactactctt
     3781 tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga agcaaaaatg gtggaaacta
     3841 tggaaataac tggaggaaac attcatgaca agaaaaaagc tcatatttgg ttctatttta
     3901 ctaggaattc taggcttcag tccgatgtta ttgtcagtga tcaataaagg aaataaacat
     3961 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagagtt atttgtaaaa
     4021 gaaacggata aataataaaa aaagactgct ctaacgagca gcccaaaatg agattatcct
     4081 agtttagcaa cataaaccat ttattagatg aaaatgagat acaaacaaag cgtatcaaat
     4141 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaattagata
     4201 tagataaccg cgaaaaatca agccttaaaa agagcttttt acctagacca atccaaaatc
     4261 acaaagtgag cctctgtatc ggttgataca agggctactt ggcatagtcg acattatgtt
     4321 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     4381 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     4441 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     4501 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     4561 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     4621 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     4681 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     4741 agtattttca gacgctgata atacgattaa atatatttat accgtc
//
LOCUS       DACCWK010000135         4747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCWK010000135 DACCWK010000000
VERSION     DACCWK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.141"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(136..1098)
                     /locus_tag="IVW65_002478"
     CDS             complement(136..1098)
                     /locus_tag="IVW65_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7901325.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="IVW65_002479"
     CDS             complement(1100..2539)
                     /locus_tag="IVW65_002479"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7901326.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="IVW65_002480"
     CDS             2724..4670
                     /locus_tag="IVW65_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7901327.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1510 a          813 c          779 g         1645 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataac
//
LOCUS       DACCWK010000136         4677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCWK010000136 DACCWK010000000
VERSION     DACCWK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.142"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(97..396)
                     /locus_tag="IVW65_002481"
     CDS             complement(97..396)
                     /locus_tag="IVW65_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7901328.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            747..1379
                     /locus_tag="IVW65_002482"
     CDS             747..1379
                     /locus_tag="IVW65_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7901329.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1502..2140
                     /locus_tag="IVW65_002483"
     CDS             1502..2140
                     /locus_tag="IVW65_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7901330.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2259..2331)
                     /locus_tag="IVW65_002484"
     tRNA            complement(2259..2331)
                     /locus_tag="IVW65_002484"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2296..2298),aa:Thr,seq:cgt)
     gene            2452..2694
                     /locus_tag="IVW65_002485"
     CDS             2452..2694
                     /locus_tag="IVW65_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901331.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2833..3462)
                     /gene="upp"
                     /locus_tag="IVW65_002486"
     CDS             complement(2833..3462)
                     /gene="upp"
                     /locus_tag="IVW65_002486"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7901332.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3596..4543)
                     /locus_tag="IVW65_002487"
     CDS             complement(3596..4543)
                     /locus_tag="IVW65_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7901333.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
BASE COUNT         1451 a          874 c          759 g         1593 t
ORIGIN      
        1 gaataaaaaa atacagactc tgttcaaata aatgaaccta gcctgcatta tgagattgac
       61 tatacttttt aattcattga gatgaataac tcgtttttat aaatgcccaa aagtaatcgg
      121 tgccgaagcc attcctcgac ctaattcttt ttcaaatttt cctattgttg atatctccat
      181 ataaaacggt gatactggtt tagacaattc tcccctgatt gctaacggac gtaaactacg
      241 ttccaaatta tttatgactt taggataata ctgacctttt tctaagcctg tctttgcgtc
      301 tatcaaaagt atacgttcct tttctttgat ctctggattt aaaacgagat catataattt
      361 tagtaccatg ttttctattt cttcactttt agacatcacg atttcctccc aaaaatatat
      421 ttcttcccac tacattctat aaacaaagta tacgctacat ctagattttt ttaaatcatt
      481 actggattgt taaaaccttt caatacactg ttatggcaac gatttttatt ttaaaaacat
      541 tctaaattaa atcagtaaaa atttttattc taagattttt attagaatta cgcctaattt
      601 gtgtctctca gggcaattct aagaacgttt caagagatag cgacactatc gttatcgaaa
      661 aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg tttagctttt gttaatatga
      721 ctttatgaaa atggaggtaa ttaaatatga tttttcctaa agttaatgaa gcaagcttga
      781 ataaaggatc cttttttcat atctatgaaa acgaagattt tgaactgtat aatatccttt
      841 cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc aattatttac aaatctattt
      901 tcaagtatgc attttttgct tccaatgatt ttcaaagaat ggagattacc gactgtatat
      961 tcgaaaactg tgatttttca ggctgtatat tgacaaaaag catgttgcat cgcgtgaaat
     1021 ttgtcaattg taaattaaca ggtactaaat tgatggaaag ttatattggg aacacgttat
     1081 tcgagaattg taaaatggac tatgtaaatt tatctggttc aaacataaaa gaaagtacct
     1141 ttgagcactc tgtcttatca agcgcagatt ttgtagactg ttccctcact aaaataatgt
     1201 tttctactaa tgatttagaa aatgctagct tttttgatac tgatttaaaa aatatcgatt
     1261 taagtagcaa ctcctttgaa atgattgaag tacgctcgga acatctaaaa aattgtacgc
     1321 ttaatcagtt gcaagcactt ggttttgcta gaaaattttt acaaataaaa gtagtataat
     1381 caaaatgaat atataagata aatttttagc aatacaaata tccataaaaa aacattgcct
     1441 attttaactc ttcatgtata ctgactctat cttcaaaaac tgaatagtag gagttattat
     1501 catgaataac attttaggaa aaaaaataga acttaaaaaa gggatattgg gcactattgt
     1561 cgttgtactc ggtttgttag ggttgctatt tgcgccatcc attattagca ttttttcttt
     1621 aactatcact ggatttatag catttacact tgtttttggt aaaaaagata cgaaaaaaat
     1681 gttctcgaaa cctgtcgctc ctgtcaaaaa tatagccaaa tattttttta tcaatgtagt
     1741 aatcagtgcc gcagtctcgg tatttttgca acaaattttg aaatggggtt taacaggcaa
     1801 tccaataaat gaggctttca gcccgttatt atttattatt ttgcctatca tgatacttgg
     1861 agaagaattg ttctcagtgt attttctcgc tattttttct agtaagttca gcattcctgt
     1921 cgctagtttc ttgtcagcaa tcatctttgg atttatccat tacagtactt atgataatgg
     1981 aaatatttta cacactgtag cacacatcct tttgatacaa ggggtggcaa gattgttatt
     2041 caaccaagct gccattaaaa gcaactcgat cattacaagt tgggcagttc atgtgatatt
     2101 tgatttcaca gctatattgc tagtcgtttt attctcctaa atttttatag aaataataca
     2161 tgctctattg ctaagaaagc atactttatt tcctttaaac aaaaagacag atgtatcaat
     2221 ggattaaaaa tccttgatat atctgtcttc tcttttaatg ccagctatag ggatcgaacc
     2281 tacgacctac gcgttacgag tgcgttgctc taccaactga gctaagctgg ccttatccaa
     2341 aagaaattat atactctatt tggctgattg taaagagtcc gatggaaaat tagtttaatt
     2401 tcatgatatt tttcataaaa cgtggtataa ttgatgtgaa aggcaggttt tatgaaaatg
     2461 gcaacttttg gaaaatttga tacaactatc gtaccatcgg aaattggtga aaaattcaag
     2521 atcaatactc acgtaatgtt cacaattcac ggcaagtcgc aaactggaac gatttcaaag
     2581 caattgaaaa attccgctgt tgttgagatt gatgaaactg cagaaaatgc gaacttgatg
     2641 acgcaaagta agggtgtagt aatcgttaac tacaaagaac taaaaaagat ttgaatttga
     2701 ccatctctgc atttttggga tggttttttt atatgcaaac gaaagctgtg aaaaagctgt
     2761 tctgcatcaa acgttaaaaa tgaccaaaaa aggatgtgac aaaagttgtg tcacaccctt
     2821 tttcgtattt acttattttg taccaaacaa acggtcaccc gcatccccta gtccaggaac
     2881 gatataacca tgttcattca atttttcatc tagagaagct gtatagatgt caatatcagg
     2941 atgcgcttct tgcaaagctt ttactccctc aggtgctgct accaaacaaa caaatttaat
     3001 gttcgacgcg ccacgttctt tcaatgcatc gattgccatg attgctgatc cgcctgttgc
     3061 caacattgga tctactacaa acaactgacg tgcatcaatg tcttctggaa gtttgacgaa
     3121 atattcatgt ggttccaatg tttcttcatc tcgataaaga ccgatatgtc ccactttagc
     3181 agctggaatc aattccaaga taccgtcaac cattccaata cctgcgcgca agattggaat
     3241 aatcgctact tttttaccag acaacgtttt ttgtgttgtt tcagtgatcg gtgtttcgat
     3301 cacgatgtct tccaatggca tatcccgtga tacttcatat gccatcaaca tagcgatttc
     3361 atcgactact tcacgaaaaa cctttgttcc gcagtttttg tcccgaataa tagttagttt
     3421 gtgttgaatc aatggatgat cgatcacttg aaatttgccc attttttaag ctctcctttg
     3481 ttctgtcgtt tctttttaat tgtaaaacaa aatcaagtga ttgactagtc aaactcgcca
     3541 tttttcctaa gaaaatccgt ttttgaaaag aagttttcct gcggacaagc aaagcttatt
     3601 cttttttctt gatccaagaa gtagataatg ccattgctgc acgcgttgcc tttgtctgat
     3661 ccatagcatt taccatgaca aaatcatgga tgattccttg gaagcgtact tgtgtaacag
     3721 gaacccctgc atcacgaagt ttgcgcgcat aagcctcacc ttcatctctt aatacatccg
     3781 cttctcccgt taaaatcatt gctgaaggaa gatcatttag ttcatttata tttgctctca
     3841 aaggcgaagc tgtgatttca gcacgctctt cttcattcgt cgtatattgg tcccaaaacc
     3901 acatcattcc ttctcttgtc aaaaaatagt tttctccgaa ttcatgatag gaatctgtat
     3961 caaaatcagc gtttgtgact ggataataca gcaattgctt ggatattggg aggccctttc
     4021 gttcttttgt taagatcgtc atgactgtag ccatgttacc acctactgaa tctcctgcaa
     4081 cgattagatt ctcgagatcc agattttttg acttggctaa tttattcagc tgttgcaaca
     4141 cagcataatt ttgttcaatt gctgttggat attttgcttc tggtgatcga ctatattctg
     4201 gaaagacaac gacggaattc gtacgaaccg caagttctct gatcaattta tcatgggttt
     4261 gagcactgcc aaaaacccag cctgctccat gaatataaaa aataactggt aatttatcca
     4321 tattcccttc tggtcgtacg agacgaatat tgagttttcc ccattcgcca gtatccacca
     4381 tcaagtcttc aatatcgacg gaatatttgt tcacaggtga ttcttgtact tcttctagta
     4441 aaactcttcc ttcttccggt gctagttcat agatacgcgg atgaggcgca tttgcatcac
     4501 tgaatgctac tgcttccttt tctaacgaga tacgatcagt catgactttt cctccctgag
     4561 atttagtgtg aagactaaaa ttacacctca tatactagag agcatcaccg atattgtcaa
     4621 agaatccgtt ttcccaaaaa ataaacagaa aagcccaaca ttgaactttt ctgttta
//
LOCUS       DACCWK010000137         4632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCWK010000137 DACCWK010000000
VERSION     DACCWK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.143"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(52..522)
                     /locus_tag="IVW65_002488"
     CDS             complement(52..522)
                     /locus_tag="IVW65_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7901334.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IVW65_002489"
     CDS             complement(681..1139)
                     /locus_tag="IVW65_002489"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7901335.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2454)
                     /locus_tag="IVW65_002490"
                     /pseudo
     CDS             complement(1310..2454)
                     /locus_tag="IVW65_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2467..3384)
                     /locus_tag="IVW65_002491"
     CDS             complement(2467..3384)
                     /locus_tag="IVW65_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7901336.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3812..4375
                     /gene="efp"
                     /locus_tag="IVW65_002492"
     CDS             3812..4375
                     /gene="efp"
                     /locus_tag="IVW65_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7901337.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1452 a          845 c          876 g         1459 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatctttt ggttagaagt cactaaacca gctactacat cgctgtgtcc
     1861 gcctagatat ttggtaccgc tatggacaac gatatcagaa ccgagatcta aaggtctttg
     1921 aaaataagga gtagcaaatg tattgtcagc aataactatc aatccatgtt gatgagccag
     1981 agtggcactt ttttctaaat cagtaatctt tagaagcgga ttgctaggtg tttcaagata
     2041 aagtgctttt gtattgggtt tgatggattg ttctatttta tcaagattac tcgtatctat
     2101 gatcgtgtac tcgagaccat ttttagtaag aactttgtca aatagacgga atgttcctcc
     2161 atagacatca tctcctaata agatatgatc gccagcttga aaaagagaaa aaacggcatg
     2221 aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca ccgccttcca aatcagcaat
     2281 cagttcttcc aatgcaaaac gagtaggatt accagatctt gaatattcat attgtttagg
     2341 ctggccgacg ccatcttgcc gataagtgga tgtttgataa ataggtacac tgacagctcc
     2401 tgttgtggga tcttcactga tgcctccgtg gatcaatttt gtttgaatgt gcatgtttct
     2461 ttcctcctat aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt
     2521 aacgacacga tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc
     2581 accactcgaa cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa
     2641 cccttctaca tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg
     2701 aatgaattct acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac
     2761 agagccctct ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt
     2821 agaagttcca gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc
     2881 tttgatttca tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt
     2941 attttcaaat tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt
     3001 gctgatggca ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc
     3061 tttcatcaat tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa
     3121 tttgtatgtg agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat
     3181 gatcgtcgtt ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc
     3241 caatctatct ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga
     3301 acctttaggc atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc
     3361 ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc
     3421 tgactattaa atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag
     3481 ttacctggta ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt
     3541 tatgcaccat acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc
     3601 ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa
     3661 gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga
     3721 gaccttaaaa gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg
     3781 attatgataa agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg
     3841 tatgactttc gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc
     3901 tggtaaagga aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata
     3961 cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt
     4021 acaatatttg tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca
     4081 atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga
     4141 agtaaaaatc caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt
     4201 attacgtgtt gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa
     4261 gccagcgaca atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga
     4321 attgttggaa atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc
     4381 taacggattg ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat
     4441 ctgcctttca agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt
     4501 ttatttatat tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt
     4561 atgaagaaat tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg
     4621 aagcgaaaat at
//
LOCUS       DACCWK010000138         4597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCWK010000138 DACCWK010000000
VERSION     DACCWK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.144"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            76..894
                     /gene="purR"
                     /locus_tag="IVW65_002493"
     CDS             76..894
                     /gene="purR"
                     /locus_tag="IVW65_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7901338.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1397..2770
                     /gene="glmU"
                     /locus_tag="IVW65_002494"
     CDS             1397..2770
                     /gene="glmU"
                     /locus_tag="IVW65_002494"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7901339.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2864..3835
                     /locus_tag="IVW65_002495"
     CDS             2864..3835
                     /locus_tag="IVW65_002495"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7901340.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3841..3943
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4009..4578
                     /gene="thiT"
                     /locus_tag="IVW65_002496"
     CDS             4009..4578
                     /gene="thiT"
                     /locus_tag="IVW65_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7901341.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
BASE COUNT         1508 a          804 c         1027 g         1258 t
ORIGIN      
        1 ataaaatgct cgaatttttg aagaaaaact tatataatgt aaaacgataa aatttcttaa
       61 aaggagttgg aataggtgaa agtacgtcgc agtgaacgtc tagttgacat gacacagtat
      121 ttattgaatc acccgcatga attgatttcc ttaacaagct ttgccgaacg ttatgaatct
      181 gctaaatctt ctatcagtga agatttggga atcatcaaga aaacatttaa agaacgaggg
      241 tatggaacct tggaaacaat cccgggtgct gcaggtggtg tattgttcat ccctgaaatt
      301 tcatatgaag aggcgaaaaa atatattgaa tctctagcag aacgtctatc agaacaagac
      361 cgtctgcttc caggaggata tgtttatctc tctgatttgt taggagaacc tgaactgctg
      421 cgtcaagttg gaagaatcat tgcttctaaa taccttggtg aaaaaatcga tgctgtaatg
      481 actgtagcca caaaaggcgt acctattgcc caagctgtgt cctattatct gaatgcgcca
      541 ttcgttattg tacgccggga ctcaaaaata acagaaggat caacagtaag tgtcaactat
      601 gtgtctggat cttctgaaag aattgaaaag atggaactgt ctaagagaag tttgaaacgt
      661 ggttcacgtg tccttgtagt ggatgatttc atgaaaggcg gcggaacagt caacggaatg
      721 aagagcttga ttgaagaatt cgaatcagaa cttgttggca tcactgtatt tgccgaatca
      781 aaattcaacg gtcatcgcgc catcgatgat tatacgtcac tgctttatgt caaagatgta
      841 gatactcaaa cgaaaaatat tacggttgtg ccaggaaatt atttttcaga ataagaggtt
      901 gtgaaaaagc cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat
      961 tttttcataa atttggctta ataccgagaa actggctttt gaacaccgtt tatctagagg
     1021 ttgtgaaatc agcgtcctgt cctgagtatt agctcaataa acggaaaaat tgctcctcat
     1081 attttgccgt ttattgagct aataccgaag gtcagctgat tgaaaaacgt ttaatgaaga
     1141 ggttgtaagc ctaccgttta actctaagtc acaagaaacg atttcaaaaa acagcttttc
     1201 atgttttatg agtagtgtga cttgtgaccg aggagttggc aggagagcac cggttaccaa
     1261 atagataggc ccgttatggt ctatctattt ttttatccag aaaacgctgg gataaaagaa
     1321 aatcacttgt tttgttgaaa aaatgggtat actgggataa actgaaatca gcaaactata
     1381 tttaggagtg acgcagttgg acgcacgtta tgcaattatt ttggcggcag ggaaaggtac
     1441 ccgcatgaag tcaaaattat ataaagtttt acatcccgtt tctggtaaac ctatggtaga
     1501 acatatcatt aatcgggtca gtgaaacgaa accagatgaa gtgattacga tcgttggaca
     1561 cggagcagaa caagttaaag cacaactagg agaacgcagt aaatatgcgt tacaagcaga
     1621 acaattggga acaggacatg ctgttttgca agctgcttct ttcttggaag gaaaaaaagg
     1681 aacaacgtta gttatcagtg gcgatacacc attattgact actgaaacgc tgaacaatct
     1741 ttttgaatat caccaaggaa aaaatgcaag tgcaacgatc ttgacggcac aagcagaaaa
     1801 cccaacagga tatggacgaa tcatccgtga ccatattggg atcgtagaaa aaatcgtgga
     1861 acaaaaagat gcaacaccag aagaagctct tgtacaagaa atcaatacag gaacttactg
     1921 ttttgacaac gaagctttgt tcgatgcgtt aagcaaagtt ggaacaaaca atgctcaagg
     1981 tgaatactat ctaacagata tcatcgaaat cttaaaagaa gaaggccata cagttgctgc
     2041 ttatcaaacg gatgattttg aagaatcaat gggagtcaat gatcggatcg cgttagctaa
     2101 agcaaatgag atcatgagaa agcgaattaa ccagatgcat atggtcaatg gcgtaagctt
     2161 tgtcgattca gcaacgacat acatcgatgc aggcgtagaa atcggtccag acacattgat
     2221 cgaagcaggt gtacaaatcc aaggaaatac agtcattggt tctgattgtg tcatcgggtc
     2281 acattcaaaa atcgtagaca gtaggattga ggatcacgtc gtcattgaaa actcagtcat
     2341 tgaaagcagc catgtcaaga aacatgcaga tgtgggacct tatgcacatt tacgaccaaa
     2401 agctgaaatt ggcgaaaacg tccatatcgg aaactttgtg gaagtgaaaa atgctcaaat
     2461 cggcaaagga actaaagtcg gtcacttgac ttatgttggt gatgctacgc tgggagaaga
     2521 aatcaatgtc ggatgtggtg ttgtcttcgt caactatgat ggaaagaaca aacatcatac
     2581 gactgtcggc gaccacagct ttattggttc tagcacgaat atcatcggac ctgtcgaggt
     2641 agctaaaaac agttcgattg ctgccgggtc aacaattaca gacaatattc cagaatatgc
     2701 gctggcaatt gcgagagcaa gacaagtcaa taaagaagga tatgcaaaaa aactccctta
     2761 tctaaactaa gacttttgca aaatcacttg atttatttaa aaaatatgac tactatttat
     2821 taggtagaat tctagagttg tgaaaaagaa agtggaggtc cccatgtcaa aacattactt
     2881 tgacccaaga ttaaaaattt ttgcgttaaa ttccaatcgc ccattagcag agaaaattgc
     2941 agcagcagtc ggcgtcgagt taggaaaatc atctgttact caatttagtg atggagaaat
     3001 tcaagtaaac attgaagaaa gtattcgtgg atcgcatgtc tatgttattc aatcaacgag
     3061 cagcccagtc aatgataact tgatggagtt gctgatcatg attgacgcac tgaaacgagc
     3121 aagtgcaaaa acaatcaacg tagtcatgcc ttattatggc tatgcgcgtc aagaccgtaa
     3181 agcacgtgca cgggaaccaa tcacagcgaa attagtagca aacatgattc aaaaagctgg
     3241 tgcaacaaga atgctgactt tggaccttca tgcagtccaa atccaaggtt tctttgatat
     3301 tccagtcgat catttgatgg gtgcgccact tattgccaat tatttcttag aaaaaggaat
     3361 caaaggcgat gatgttgtcg ttgtttcacc agaccatggt ggtgtcacac gggcacgtaa
     3421 attagcggaa tttttgaaat ctccgattgc gatcatagac aaacgccgtc caaaagcaaa
     3481 tgttgcagaa gtaatgaata tcattggaca agtcaaaggc aaaacttgtg tactgatcga
     3541 tgatatgatc gatacagcag gtacgatcac attggctgcc aatgctttga aagaagctgg
     3601 tgcaactagt gtgtatgctt catgtacaca tccagtatta tccggccctg ctttacaaag
     3661 aatagaagat tcagctatcg agcgtctagt tgttacagac tcgatctatc ttccagaaga
     3721 tcgtaaaatc gacaaaatcg atgaagtaag tgttggcgga ctaatgggag atgcaatcaa
     3781 acgtatccat gaaaacaaac cagttagtcc attgtttgaa acaaaaaata aataataaat
     3841 aaactcacaa aggggagtcc aattgggctg agattgaatc tatttctaaa cccttcgtac
     3901 ctgtatcggt tatgcgagcg taggaattgt gaataaacag ctgtttgact attttcgaaa
     3961 gctgcttcct cctttgtgac ttttttattc aaaggaggaa gtttttttat gggaaaaaat
     4021 agagtttggg tagaaggaac gattgttgca gcgttagcga tggtgttgtc gttgattccg
     4081 atccagatcg gcagcagttt ttctatttca ttagggcaga ttccattgac tctttttgcg
     4141 ttaagaagag ggacaaaggc agggatgctg gcaggtttca tttggggcat cctacatttt
     4201 ccgttgggac aagtttatta tttaagtgtc gttcaagtgt tgattgagta cccgattgcc
     4261 tatacttttg caggcgcagc agggctcatg gcttcaaatg tccaaaaagg ctatgtagac
     4321 gataatacga aacaaattag aaaaagcatc gtgtttggtg cattgattgg cgcactttcc
     4381 agatattttt ggcattttat tgcaggagtg gttttctggg gagcgtatgc actatggggg
     4441 atgaatcctt ggctcttttc tttcgtaatg aatggtgcca gcgggttgag tacagctatt
     4501 gtaacgatca tcgttttatt agggatccaa caaacgactc cttcgatttt tgtgccagat
     4561 aaacgacagg agctgtaaat aaaaaagaag caagaaa
//
LOCUS       DACCWK010000139         4535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCWK010000139 DACCWK010000000
VERSION     DACCWK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.145"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..725)
                     /locus_tag="IVW65_002497"
     CDS             complement(<1..725)
                     /locus_tag="IVW65_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7901342.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGG"
     gene            complement(888..1481)
                     /locus_tag="IVW65_002498"
     CDS             complement(888..1481)
                     /locus_tag="IVW65_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901343.1"
                     /translation="MRVKGQNLVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(1482..1814)
                     /locus_tag="IVW65_002499"
     CDS             complement(1482..1814)
                     /locus_tag="IVW65_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901344.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(1864..4395)
                     /locus_tag="IVW65_002500"
     CDS             complement(1864..4395)
                     /locus_tag="IVW65_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901345.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
BASE COUNT         1220 a          934 c          711 g         1670 t
ORIGIN      
        1 cctcctggat cttttagcgt ttggtggtgg aacgggaaac ctccgccaat gtttaacgat
       61 ggtagtgatt gccctttgat atttccgaac cattcagctt gttcatcgaa catggtaaac
      121 atcgattgcc aatctcgttt ggttttatgc agataagtca ccgcacgata atccatccct
      181 ttgagatttt cgatcagttc ctgcgctcgt tcatctactt cttgaatcgt gtttccgtaa
      241 acatgtactc tcactttgac ttgtttagaa atttccccat tagaagtcaa ttttcgggca
      301 tattccgcta attccaggta ttcttccgca cttttatttc ggtcagtcgc ttttcgttca
      361 ttatctgatc gatcatttaa ttcatctaat gcgcggttga tttgatcaat cactttttgc
      421 ttttcatctg tgctaacatc gagtgtggcc actgtatgag gattgttcat gagattggcc
      481 aaccaaaaaa gcgtaggatt ttttgtgtag tgataaactg tcacacatcg tgaataacca
      541 ttaccatacg tcacatattc ttcatcaaaa tttaacccac cttgtggcat cacttgtttt
      601 aaaaaatcta aatcataccc tttggatttt aaaatttcta tttcccgatt tcctagttgc
      661 tttaatggat caagtacttt ttgatcattt tgtggtgaaa aagctgtttt aattgcttct
      721 aacatgtaca gtcacctctt cttgtttgat tctttacttg gttaaaagat tgcccttttc
      781 ccaacttacc caggcttaaa gtgtgaggaa attttctagt ggtctcttcc ttcggaacat
      841 ctttttctaa ccatatcaac ggatatttca ttctctcttc tccattccta ctcatcattc
      901 atattgttca ttcgatacag aatcttgatc aattcttttt tgtagagtgg ttttaaccct
      961 aaaatgccac tagatcgcat taactgacgg tttctggttt gtaaatcctt gattgtcttt
     1021 ccttcaatga cgaaaaagaa gcttaagtca ggacgttctt gttctgctcg tttcaattcc
     1081 tggatttctt cgactaattt cttttcatat ttttttaatc gttgtagatt tcttcctaag
     1141 gactgttcca tacgattttt atctaataat tgccgctttt gcacgatttg ttccgttaaa
     1201 tccgtctctg tggtcaaact aactaactta attgttggca catacgtacg catgacggtg
     1261 gtcaactgat tcattcgttc tagtttctct tgttcattta atccttccac gtctgtagaa
     1321 actacacgat aataacgttg aaaatgatct tctttagtaa ctaagactcc ctcttctgta
     1381 caggatttat aaggaaataa atccaagata gatcgttctt ctggttttcc aaactgaaaa
     1441 atggatttct tatcaactaa attttgtcct ttgactcgca tttattcatc ctccttatac
     1501 gtaccatgat aatttcggtt aggatctaac gcatgataat ggttcttgtc cgtaaaaaaa
     1561 atagactcga taatcgcaaa aatttgtcgt tgacctgggt tattataggg tcgccaaaca
     1621 atcactagag ctatcgcaat ccataagcca taattcaaga gtgcaaaaaa gagtggtaaa
     1681 tggacaaggt tattgactat tttcgcccaa aaaaagcaac caccaagcgt taatagatcc
     1741 aaaaagaaaa aggcgaaaaa cttccattcc ccactaaatc ttggagggtt tttcatgttt
     1801 atatatttat ccataattga tttctccttt tcatataaag aaaaggccta acttgtaggc
     1861 cttttatggt ttgatatcct gtttgatatc cctcaatagt tgttctttca atgcattgtt
     1921 cctgttttta gtttctcttg tcacatttat ttttgttggt gcagtaaatg ccacatttgc
     1981 tggcttgtaa cctgtatgaa cgttttgaat aggttttggt gcagcttctc ttgtcacaac
     2041 attttgaacg gacgcattcc ctgtctcacc actttcagat aaattgtaag aaacagttgt
     2101 cagatttcca ttttcaggca aacgacttgg tttcatttga acagtacctg caggtgattg
     2161 atgaacaaaa ctttgatcat tctccttgaa ttgattcaaa cgactatctt tcattggtaa
     2221 attttgtgcc ttaggtaaac caattttcgt tgatttttca gttccaactt cgattccatt
     2281 atcatttggc agaacgatgg aatcctcatc cgtttgtact tcttgatttc gttttggtaa
     2341 atctgaaatg cgtttttcat tttcggagtt aacagatcta taccgatatt gttctggatc
     2401 agtttttggt aactgtccct cattttctgc tttgtcttct tgatttactt ttgataaatc
     2461 tgaaattcgc ttctcatttt caaggtcact gttaggttta tagcgatatt gttctggatc
     2521 agtttttggt aactgtccat tttttccttc ttctgaattt ctttcttcta tggaattttt
     2581 ctcttcaatc gaattttcat tagagtgcaa cgcttcttgg tgatctttga ctgaaggaat
     2641 cactatacct tcttgatttt ctttttcggt tgttgtatta gcttttactg ctgcattaac
     2701 ttgtccttca ccatacgcat cttttagtcc ttctttgact tctgaagctt tttctttttg
     2761 tttttctagt gcttcggatg gattcttcac agcttcagcc aattctttag ctctcgtgcc
     2821 gtctaatttc tcttctaagg tgtctttggc tttttctgta agatcttcag ctgttccttt
     2881 taacccacgt tcattgacgt aaccaagacc ttctgcggct ttagaaacac catttttcgc
     2941 tttcccgaac atttcggaaa ctgttttctt actattaggt acagttgtag ctttatcgtc
     3001 tgattgttct tccccgttat caggggtaac atcttttcga tggccaaatc catttttacg
     3061 aatatcattt aaatggctta aaccactctt accagcactt gcaaggtttt tagctccgct
     3121 acccacattt ttcgcaatat ttcctaaggc aaacatgcta agaagagaat tactgccgtt
     3181 tttcagactc gtatcaacac cgaaaatctt cgtaaccgat tgagagcctt tgaatagcgc
     3241 aatagttagc acaattaaag caacaacata caagaatcca ttgatctttc ctgccgcatg
     3301 caaatcaccg atacctgaaa gcattaatgt atagattcgt aattctaaaa tcgtaaacgc
     3361 caataacaag aaggattgag aaatatcttc tatcgcactt ttcatccgtt ggcctgtctc
     3421 aaagtccgtt gacgcggcaa tcactaaata gaatttttta aataccagct caataatgca
     3481 agtaacgatc gtaaatagga taaataaata ggccaccgcc aaagaagatt caccagctaa
     3541 gataatacct ggtttactac tgtatcgacg ataccctgaa gtatagacat attttgctaa
     3601 gccttcgtca ctaatttgaa caggaatttc tttcccatta tcatctagtt ccaatcggta
     3661 ttgtaacgct cttaaccgat ccttttgtaa atctgataaa ccactcttat ctttatccgc
     3721 catttcttta gcttgtttag gagtaataat cccattgata ttcgctgttt taaaattttt
     3781 cgctgttaaa gcatttcttt tgtctgttgg cagggattct actttattcg ggtcagttgc
     3841 tagtaatgta ctgacatcga tcacattatt ttgaataatt tgaaaagcag gtgaacccgt
     3901 tttatctgct ttatttgctg cagtgacgtc tccgtaaaag cttttactga tatttaaccc
     3961 ttcatcgatt aaagaactcc caccaaaaat caagatcatg gccataaata aattaaccaa
     4021 gatatttttg acttttggtg ggtgtttact cataaaaaat ttcaagccta aataaattaa
     4081 tgtgacgaca caaaaaagag ccaatacctt actaatcaaa ctttgaaata gtgcatttac
     4141 cccagcggcg ttcaataagt cttttaaatc aaagctttga taaatggttt tttctagtaa
     4201 agtattgaga ttatataatc ctttaatcaa tccccacaga aaagtgttcc aaatccaact
     4261 aaatacatct tgatactgca gataattacc ataatcttgt aagactttcg ttacggtttt
     4321 ggggtttgca tccccttctc cgaataagcc tgaccaccag tcggctatgc cagaaaaaat
     4381 tcctccaatg atcattcttt caccaccttt tcagattcca ataacaaccg tttcccaatc
     4441 atttcagaaa tttctttatt tttgatcaac ttttcaacat attcttgaag tcgatctgct
     4501 gcttgttctt cgtaaaattg aattaacaat ttttt
//
LOCUS       DACCWK010000140         4466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCWK010000140 DACCWK010000000
VERSION     DACCWK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.146"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            575..1009
                     /locus_tag="IVW65_002501"
     CDS             575..1009
                     /locus_tag="IVW65_002501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901346.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2299..3222
                     /locus_tag="IVW65_002502"
     CDS             2299..3222
                     /locus_tag="IVW65_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7901347.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3238..3777
                     /locus_tag="IVW65_002503"
     CDS             3238..3777
                     /locus_tag="IVW65_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7901348.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1673 a          669 c          753 g         1371 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaaataa tgagagttta ttcttgaaaa agcaacgcgc
      241 ttataaagcg ctaccaaaat tttatcatat tttataaaaa ataacaatcc agtaaaaaat
      301 gactggtaaa aattcacaaa gaaacggaaa gaagcaacta cagaaaatca agccttaaaa
      361 agagcttttt acctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga
      421 ggggcaactt ggtctagtat acattatgtc tactaacctt taaaataaca cgttttataa
      481 aataaaccct cttgacaata tttaattttc tgattattct aaatatatac agaaagcgct
      541 ttcctaggat tttatatcta aggaggaagt aacaatgaaa aataaaatta ttaaaattat
      601 tacttgtcta tttttgttcg gtatgattgt tggagcaagt actccgttaa atctgtttcc
      661 ttttagtgct agaggtataa aagtggaagc tgcaagttca cgatataatc ataatcatcg
      721 tggatttact tgctataaaa tgaactatta tgttactaat aaaatgtgta gagatttgaa
      781 aaaaaattat aataagctaa agactcctgc tcaaattgca tcttttattc ctattggtgg
      841 agttggaaca tgggttatta caaatgcttt tagtagtgct gttgatgcaa tgaatgtctt
      901 tgtaagagct gcaaatcaag gaaaaggtgt acaattaaca tataatgctc actttagcaa
      961 tacaacatca tatcaataca atgattacgc tcgttatgta ataaaataat cagaaagtga
     1021 aactatctca tgaataaaaa gattttagct gtaattattt caataatagt tttaatcact
     1081 atgttcttca tatttaggct aattttcaat ataagcttac aaaaaagtat tctttatcta
     1141 attcctatag catttatctt agcaattttt agaagtattt acggaaataa aaactctaaa
     1201 tagctaaaaa taaatcatac taaattttac aaaaagtggc tagcattttc tagccacttt
     1261 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1321 agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc ttgccaaacc
     1381 cttttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1441 ccgtaggacg tactgaaagg gtgggactta tgagcaaatt aatgcaaaat ttggctagca
     1501 aaaaagctaa aaaatatatg ctaataatcg agcgaaataa acactttttc aagtgagcga
     1561 atccgccatg acaaagctga agcagtttat ttcagctttg tcatggcgtg tgccgacttt
     1621 tttaaaagtc taggcacacg agcaaatatc tttatcaagc tctaatttgc gttttaagac
     1681 gtttgaaacg cctaagtata taaatatata agaataggtt taagagtgct taaatcgaat
     1741 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     1801 gttttaagaa caagataagg gatttatact aggactgtct taaaatgtct taaacgaacg
     1861 ctcagagctt ctcaaagggt tccttatgaa cggcttttag caaaggagcc ggaggcgact
     1921 gcgcagtata atgtgagagc agagcgatca caccttttta gcgaaccgta gggagcaagg
     1981 tttggaattg gcagttttga taaattcgcg aacctgttta gagaaaaaag gagtgatttt
     2041 ttaggcggga aaattagcct cccctctttt tatatctctt tttatatctc tttttatatc
     2101 tcttttatgt cggctgtgtc cttactctcc caagcaattc agagtttttt caagtaggtt
     2161 ttctacgctt ttcaagtagg ttttctacgc ttttcaagta ggttttctac gcttttcaag
     2221 taggttttcc gacattattg agtattttta taaagtatgt tacttttatt tcatacttta
     2281 taaaagtagg agaagcttat ggcaaatgaa attgtaaaat atgaaaatag attaaatcat
     2341 attcctctga gaaaatttaa tagtcgtgaa atgaacttat ttttttcaat tgcttcacgt
     2401 gtacgtgaaa aaggtgtaga tgagattatt ttttcatttc acgatttgaa aaaactaagc
     2461 ggttatacag aacgtgggga acaatttata aaagatctta gtagcacgta tgacaaatta
     2521 ttaagtatta atgcttggaa tgatgatgga aatactttaa ctaaatttgt tgctttcaca
     2581 aaatatagca ttatacgaga taaacaactt gttataattt cagttaatcc agaatttaaa
     2641 ggacttttta atcaattaaa gaactggaca agatttaatc tagagcaatt tactaatttg
     2701 gatagcacat attcaaaaac tatgtttcga ttattaaaac aatatcgaac tcaaggctgg
     2761 actgaatttt ccaaagaaaa attttttgat ttactagata ttcctaaaag ttattggaat
     2821 agtccgtcaa atattgacaa atttatctta aggccaatta aagaagaact cactccctta
     2881 tttaaaggat taactattcg taaaaaatat ggtaaaggtc gaggtaaacc tgttatcggt
     2941 tatcgtttta cttggaaagc tgaaattaac catgctgacg atttttccaa agggaaacaa
     3001 gaagatttac gtataaaatt atttaatatt gatcacaatg gagaattaac acgagaagaa
     3061 aaatggcgcg ctaaagatcg aatactaaat cttcccttag gcactcatga agctgatttt
     3121 aaacaacaac aacaaactga agaaaaacag gcagaaaaac gagaactttc aaacaaactc
     3181 aaacaagatt tactaggaaa tttacaaaac ttattcgatt aagcttaagg agtatttatg
     3241 gcagataaaa caatcaaaga acttgcggaa gaattaggag ttagtaaaac agcaattaac
     3301 aaaaaagttt ctgacaaaaa tcgaaaactt tggtttgcaa aaaacggaaa caagtttgtg
     3361 attaacgaaa ccggtcaaaa agctataaaa tcaatgtttt tagtcgataa cgcaaactta
     3421 aatcgaaaac tagttggcga aaaatcaaaa accaaaaacc ataaaaacac caaccaagtt
     3481 tccgaattaa aactcctttc tttcctagaa aaacagttaa atcgaaaaga tatacagcta
     3541 caagaaaaag ataatcaaat aaaacaaatg caaaaattac tcgaccagca acaacaactc
     3601 actctacaag cgaaccaaca aattgagaac ctacaagaac agctccattt aacttataca
     3661 gaagaaactc caacaaccga atcaactgcc ttttctgaaa aaaaagcaga caatacacaa
     3721 ccacttattg aaaaaaagtg gtggcaaatt tggaaatcta atagaaccaa tctctaaaat
     3781 tttctagaag ttagttcgga tagtagagga aaaagttgta ctacctgatg gggagatcac
     3841 tcaaattttt atgagaaaga aaaaatagtt tacaactgta aacaatcaat gtttatagtt
     3901 gtaaactatc aaactatatg gtgacttatt gcttaaaata ggttgaaatc tcactcaatt
     3961 tttggtggaa aaacaaaata aattctaatc gattttgttg aaaaagtcat aaaaaaacaa
     4021 atgtgatttt aacaaaattt caaaaaaatc gtttgaaatt tcgtttattt tttttgagaa
     4081 ttttgatcat cgtagggtca ttgtagaatc attatgagat catcgtggga tcatcgaata
     4141 aagtatggga aaggcttgcg gtgcttggta ttaagggcat tctccgcctc ccaggttctt
     4201 gaccattttc tctcaacgtg gtaaaaatcc cacttataag agaaaatggt caagaaccta
     4261 aacggtcggc gaagaaaaca taccaagcac cacaagcaaa aaaagttaga gtaacttcca
     4321 aaaaagaaaa attttttaga acaacttttg aaaatttaat tttgatttat atccgttcaa
     4381 tttaaacatt tgataaactg aaggctagta tttaaaatgt gtatacaagt ggtagcgtta
     4441 aaaaaagtgg aggtataacc tatgtt
//
LOCUS       DACCWK010000141         4428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCWK010000141 DACCWK010000000
VERSION     DACCWK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.147"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(543..2048)
                     /locus_tag="IVW65_002504"
     CDS             complement(543..2048)
                     /locus_tag="IVW65_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7901349.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVW65_002505"
     CDS             complement(2045..2731)
                     /locus_tag="IVW65_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7901350.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVW65_002506"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVW65_002506"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7901351.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1237 a          919 c          729 g         1543 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaac
//
LOCUS       DACCWK010000142         4384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCWK010000142 DACCWK010000000
VERSION     DACCWK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.148"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..725)
                     /locus_tag="IVW65_002507"
     CDS             complement(<1..725)
                     /locus_tag="IVW65_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7901352.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGG"
     gene            complement(888..1481)
                     /locus_tag="IVW65_002508"
     CDS             complement(888..1481)
                     /locus_tag="IVW65_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901353.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(1482..1814)
                     /locus_tag="IVW65_002509"
     CDS             complement(1482..1814)
                     /locus_tag="IVW65_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901354.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(1866..4244)
                     /locus_tag="IVW65_002510"
     CDS             complement(1866..4244)
                     /locus_tag="IVW65_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901355.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKETAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKVGLPKAENLPTK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQSIV
                     TREAAPKPIQNVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKS"
BASE COUNT         1193 a          901 c          695 g         1595 t
ORIGIN      
        1 cctcctggat cttttagcgt ttggtggtgg aacgggaaac ctccgccaat gtttaacgat
       61 ggtagtgatt gccctttgat atttccgaac cattcagctt gttcatcgaa catggtaaac
      121 atcgattgcc aatctcgttt ggttttatgc agataagtca ccgcacgata atccattcct
      181 ttgagatttt cgatcagttc ctgcgctcgt tcatctactt cttgaatcgt gtttccgtaa
      241 acatgtactc tcactttgac ttgtttagaa atttccccat tagaagtcaa ttttcgggca
      301 tattccgcta gttccaggta ttcttccgca cttttatttc ggtcagtcgc ttttcgttca
      361 ttatctgatc gatcatttaa ttcatctaat gcacggttga tttgatcaat cactttttgc
      421 ttttcatctg tgctaacatc tagtgtggcc actgtatgag gattgttcat gagattggcc
      481 aaccaaaaaa gagtaggatt ttttgtgtag tgatagactg tcacacatcg tgaataacca
      541 tttccatagg tcacatactc ttcatcgaaa tttaatccac cttgtggcat cacttgtttt
      601 aaaaaatcta aatcataccc tttggatttt aaaatttcta tttcccgatt tcctagttgc
      661 tttaatggat caagtacttt ttgatcattt tgtggtgaaa aagctgtttt aatcgcttct
      721 aacatgtaca gtcacctctt cttgtttgat tctttacttg gttaaaagat tgcccttttc
      781 ccaacttacc caggcttaaa gtgtgaggaa attttctagt gatctcttcc ttcggaacat
      841 cattttctaa ccatatcaac ggatatttca ttctctcttc tccattccta ctcatcattc
      901 atattgttca ttcgataaag aatcttgatc aattcttttt tgtagagtgg ttttaaccct
      961 aaaatgccac tagatcgcat taactgacga tttctggttt gtaaatcctt gattgtcttt
     1021 ccttcaatga cgaaaaagaa gcttaagtca ggacgttctt gttctgctcg tttcaattcc
     1081 tggatttcct ctactaattt cttttcatat tttcttaatc gctgtagatt tcttcctaag
     1141 gattgttcca tacgattctt atctaataat tgccgctttt gcacgatttg ttccgttaga
     1201 tccgtctctg tggtcaaact aactaactta attgttggca catacgtacg catgacggtg
     1261 gtcaactgat tcattcgttc tagtttctct tgttcgttta atccttccac gtctgtagaa
     1321 actacacgat aataacgttg aaaatgatct tctttagtaa ctaagactcc ctcttctgta
     1381 caggatttat aaggaaataa atccaagata gatcgttctt ctggttttcc aaactgcaaa
     1441 atggatttct tatcaactga attttgtcct ttgactcgca tttattcatc ctccttatac
     1501 gtaccatgat aatttcggtt aggatctaac gcatgatagt ggttcttatc cgtaaagaaa
     1561 atagattcga taatcgcaaa aatttgtcgt tgacctgggt tattataggg tcgccaaaca
     1621 atcactagag ctatcgcaat ccataagcca taattcaaga gtgcaaaaaa gagtggtaag
     1681 tggacaagat tattgactat tttcgcccaa aaaaagcaac caccaagcgt taatagatct
     1741 aaaaagaaaa aggcgaaaaa cttccattcc ccactaaatc ttggaggatt tttcatgtta
     1801 atatatttat ccataaatga tttctccttt tcatacatag aaaaaaggcc taactcgtag
     1861 gccttttatg atttgatatc cttcaataat tgttctttca attcattgtt cctgttttta
     1921 gtttctcttg ttacatttat ttttgttggt gcagtaaatg ccacatttgc tggcttgtaa
     1981 cctgtatgaa cgttttgaat aggttttggt gcagcttctc ttgtcacaat actttgaacg
     2041 gacgcattct ctgtctcccc actttcagat aaattgtaag aaacagttgt caaattccca
     2101 ttttcaggca aacgacttga tttcatttga acagtacctg caggtgattg atgaacaaag
     2161 ctttgatcat tctctttgaa ttgattcaaa cgactatctt tcgttggtaa gttttctgcc
     2221 ttaggcaaac caactttcgt tgatttttca gttccaactt cgattccatt atcatttggt
     2281 agatcgatgg aatcctcatc cgtttgtact tcttgatttc gttttggtaa atctgaaatg
     2341 cgtttttcgt tttcagaatt aatgttagat ttataacgat attgttctgg atcggttttt
     2401 ggtaactgac catgttgtcc ttcttctgaa tctttttctc cgatcgaatt ttcgttagaa
     2461 tgtaactcat cttggtgatc tttggctgaa ggaatcgctg caccttcttg attttctttt
     2521 tcggttgttg tattagcttt tactgctgca ttgacttgtc cttcaccata cgcatctttt
     2581 agtccttctt tgacttctga agctttttct ttttgttttt ctagtgcttc ggatggattc
     2641 ttcacagctt cagccaattc tttagctctc gtgccgtcta atttctcttc taaggtgtct
     2701 ttggcttttt ctgtaagatc ttcagctgtt tcttttaacc cacgttcatt gacgtaacca
     2761 agaccttctg cggctttaga aacaccattt ttcgctttcc cgaacatttc ggaaactgtt
     2821 ttcttactat taggtacagt tgtagcttta tcgtctgatt gttcttcccc gttatcaggg
     2881 gtaacatctt ttcgattgcc aaatccattt ttacgaatat catttaaatt gcttaaacca
     2941 ctcttaccag cacttgcaag gtttttagcc ccactaccca catttttcgc aatatttcct
     3001 aaggcaaaca tgctaagaag agaattgctg ccgtttttca gactcgtatc aacgccgaaa
     3061 attttcgtaa ctgattgaga gcctttgaat agcgcaatgg ttagtacaat caaagcgaca
     3121 acatacaaga atccattgat ctttcctgcc gcatgcaaat caccaatacc tgaaagcatt
     3181 aatgtataga ttcgtaattc taaaattgta aatgccaata acaagaagga ttgagaaata
     3241 tcttctatag cacttttcat ccgttgtcct gtctcaaagt ccgttgacgc ggcaatcact
     3301 aaatagaatt ttttaaatac cagctcaata atgcaagtaa cgatcgtaaa taggataaat
     3361 aaataggcta ccgacaaaga agattcccca gctaaaataa tccctggact actaatgtat
     3421 cgacgatagc ctgacgtata cacatatttt gctaaacctt catcactaat ttgaacaggg
     3481 atttctttcc cattatcgtc taactctagt cgatactgta atgcctttaa ccgatctttt
     3541 tgtactgtac taatatcact tgattcagac attttcttag cttgatcagg tgtaataatc
     3601 ccattgatat tcgctgtttt gaaatttttc gccgtgagag catttctttt gtctgttggc
     3661 agggattcta ctttattcgg gtcagttgct agtaacgtac tgacatcgat cacattattt
     3721 tgaataattt gaaaagcagg ggatcccgtt ttatctgctt tgtttgctgc agtgacgtct
     3781 ccgtaaaagt ttttactgat atttaaccct tcatcgatca aagaactacc accaaaaatc
     3841 aagatcatgg ccataaataa attaaccaag atatttttga cttttggtgg gtgtttactc
     3901 ataaaaaatt tcaagcctaa ataaattaat gtgacgacac aaaaaagggc caatacctta
     3961 ctaatcaaac tttgaaacag ttcattaaca ccagcagcgt tcaataagtc ttttaaatca
     4021 aagctttgat aaatggtttt ttctagtaaa gtattgagat tatataaccc tttgatcaac
     4081 ccccataaaa aagtattaca aatccaactg aacacatctt gatactgcag ataattacca
     4141 taatcttgta agactttcgt tacggttttg gggtttgcat ccccttcacc aaataagcct
     4201 gaccaccagt cggctatgcc agaaaaaatt cctccaatga tcattctttc accacctttt
     4261 cagattccaa taacaaccgt ttcccaatca tttcagaaat ttcttcattt ttgatcaact
     4321 tttcaacata ttcttgaaga cgatccgttg cttgttcttc gtaaaattga attaataatt
     4381 tttg
//
LOCUS       DACCWK010000143         4372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCWK010000143 DACCWK010000000
VERSION     DACCWK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.149"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(83..1051)
                     /locus_tag="IVW65_002511"
     CDS             complement(83..1051)
                     /locus_tag="IVW65_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7901356.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1496..2902)
                     /locus_tag="IVW65_002512"
     CDS             complement(1496..2902)
                     /locus_tag="IVW65_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7901357.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQRQPSFNKALQQQGLQTDKENGRVIWNNFRSQEVESVE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTVVKERESVSDDLRYISKQREE
                     NEREKALVAFERAEIEEEKKNMVNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENCFLRQRLERLKNAFQKSITRLSIRFGLAERDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSENEIEW"
     gene            complement(3124..3486)
                     /locus_tag="IVW65_002513"
     CDS             complement(3124..3486)
                     /locus_tag="IVW65_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901358.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENATMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDGLFGVITNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3490..3798)
                     /locus_tag="IVW65_002514"
     CDS             complement(3490..3798)
                     /locus_tag="IVW65_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901359.1"
                     /translation="MDKIDLREETVDRIELMYEWLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHRHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3807..4226)
                     /locus_tag="IVW65_002515"
     CDS             complement(3807..4226)
                     /locus_tag="IVW65_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901360.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
BASE COUNT         1246 a          986 c          600 g         1540 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt aatttttccc
     1021 actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag aaaaaagaga
     1081 tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca tcaatctcta
     1141 gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac tagtgtggtt
     1201 agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt tgctcttttt
     1261 ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc aaataccaaa
     1321 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taagttggcc
     1381 aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg gtttagccgt
     1441 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtggctacc
     1501 actcaatctc attctcagaa cgctctaatt tacgctgtga gacgttttga cgataaaata
     1561 aggttttctc ctgctctaat acttctaatt cctcggaaag ccccatatcc ctttctgcaa
     1621 gcccaaaacg gatagacaaa cgagtaatcg atttttgaaa tgcgtttttt aaccgctcta
     1681 gacgctggcg taaaaagcaa ttttcttctt tcagcttttt attttccact tgtaaatctg
     1741 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1801 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1861 tagcatttgt tgccctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1921 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct ttttgaaaaa
     1981 taaccgccat ctcttttttt acattcacca tatttttctt ttcttcttcg atttccgctc
     2041 tctcaaaagc caccagagcc ttttctcgct cattttcttc tctttgcttg gaaatatacc
     2101 ttaaatcatc agaaacactc tcacgctctt ttacgaccgt ttgacgcaat tctgaaatct
     2161 cgctcatgat ttctttatat tcgtgaatat ctttgatttt gttagtaccg actaactcac
     2221 gctcccaacc aaaatccgcc attaaccgct caaccgattc aacttcctga cttcggaaat
     2281 tattccagat cactcgacca ttttctttat cagtttgtaa cccttgttgt tgcaaggctt
     2341 tattaaagct cggttgtctt tgaacacctc gtttataccc ttcagcaaca ggaacaatat
     2401 tcaaatgcag gtgcggactt acttcgtcat tatgaataac agcattgtag acggataaat
     2461 taggattccg ttctttaaat tctttgaaat actcaactaa aattttgttg gcaatcactc
     2521 gattttgttc tgcttgttgt tccgatatgc cagtagaaga accatcatca cgagtagtac
     2581 gaaaatcatc aagagtacca atttgcacga taaattctcg ctgtggttcc aattttttat
     2641 ccttgcgaac ctttgctaaa tagtcctcga tttttctatc ggctcttttc tgctttgcat
     2701 tatatttttc tacggcttca ccgaataatt cttcatattt ttcacgaata tttgtttgtt
     2761 ccaaatagat attttcatcc gattttgtga aatcaatatg cttatgccag tcattgattt
     2821 tttggctatc agttaactca cgattattat gagaaatgct tgtattcttt gttccctttt
     2881 tgaacgaaat actaaacccc aagttaaaaa cctccttcgc gctttcgcta ataaaaagag
     2941 tgtaaacctt tctgttactc tttgtcaaag agtaacgttt tcctaattgt gacatgactg
     3001 tcacaattcc aaacgctctc cgagggattt cgctgtcaca agtgacaacg aaaaaaggag
     3061 ccgaaagcgt gtcacgctct caactccttt tacgctttat tttgcttcac gcaaaataat
     3121 cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac tcatctgact
     3181 tttcttattc gttatcactc cgaataagcc gtcagattca tcgggataat ctcgttcgct
     3241 tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt gttgctgatc
     3301 gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gattcaaacc caaaacgctt
     3361 gcgaagagcc ttgcccattg tagcattttc tttctcaatc aagcgttttt tctgttgacg
     3421 aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg ctaaagtcat
     3481 tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt ttaactgcat
     3541 aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg tttgcgctaa
     3601 atgcctatga acaaattcat tagtcaaaac tgaagatttt gctagatcag cattgataac
     3661 cgagttgaga acatccatca ttcgttcaag catttcacta taattatctt catccggcaa
     3721 attcaaataa tcagctaacc attcatacat caattcaatt ctatcgactg tttcttctct
     3781 caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt tcgatttttt
     3841 aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa agtcaatttt
     3901 tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc cgctaattgt
     3961 tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc attgaccggt
     4021 aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac accaatcaat
     4081 tccttgtcta acatgataac cccattatct ttataccgag caaggtcata acctgcattg
     4141 accagttgat tgcgcactgt atttttagat atacccaact catcagcaag gttttttatg
     4201 gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta ccccataaac
     4261 atatttaaca gcatgcttag ggataatgat tggggaatca attttattat ttttcaaaat
     4321 caactcatac tgcgttacat caagtaactt accggacatt tttttgctat cc
//
LOCUS       DACCWK010000144         4297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCWK010000144 DACCWK010000000
VERSION     DACCWK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.150"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..792
                     /locus_tag="IVW65_002516"
     CDS             <1..792
                     /locus_tag="IVW65_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7901361.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="IVW65_002517"
     CDS             889..3138
                     /locus_tag="IVW65_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7901362.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4297
                     /locus_tag="IVW65_002518"
     CDS             3141..>4297
                     /locus_tag="IVW65_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7901363.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1501 a          610 c          791 g         1395 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagt
//
LOCUS       DACCWK010000145         4164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCWK010000145 DACCWK010000000
VERSION     DACCWK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.151"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            84..1385
                     /locus_tag="IVW65_002519"
     CDS             84..1385
                     /locus_tag="IVW65_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7901364.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1412..1672
                     /locus_tag="IVW65_002520"
     CDS             1412..1672
                     /locus_tag="IVW65_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7901365.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2671..3906)
                     /locus_tag="IVW65_002521"
     CDS             complement(2671..3906)
                     /locus_tag="IVW65_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7901366.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1196 a          670 c          866 g         1432 t
ORIGIN      
        1 cttaattata taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat
       61 tttattgaaa aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg
      121 tcccaccaat ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg
      181 tttcaacatt gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt
      241 ttcctggatg gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga
      301 tgacgatggg attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat
      361 catatggatt agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac
      421 aagttcctgt aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga
      481 gttctttggg agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag
      541 tatatcgttt ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg
      601 ctgttgcccg tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc
      661 tgattcgaag cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt
      721 taaccaatat tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt
      781 tgttgtggtc atttggtatc catggcatga gtattgtagg ggctgttgtt cgtccaatgt
      841 ggctgatcat gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat
      901 taccctatat cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag
      961 caacgatcgt ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg
     1021 ttggtcgttt ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag
     1081 ctcctatgat gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga
     1141 ctattatcag ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa
     1201 catggacatt accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg
     1261 ttatcttgat catgatcaac acattgatag catttattat ctattatcca tttgtaaaag
     1321 cttatgataa aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg
     1381 tctaagtttg gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt
     1441 tatcttttta ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca
     1501 aatcaatcga tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat
     1561 tgtaccgcat atgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc
     1621 tatcaatttt atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta
     1681 aaagtggttg taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc
     1741 ttcttaaatt tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga
     1801 tgcaagcttt tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag
     1861 ccctttttta cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa
     1921 aatggaaatt ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag
     1981 tcaaatatag atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat
     2041 ggtaatatgt atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg
     2101 ctgggaatag caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata
     2161 gaacattctg acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat
     2221 gcatcggatt gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag
     2281 tggtaaatcc tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca
     2341 aagcgatgct tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat
     2401 ggtctttctt ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg
     2461 actaagaata aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt
     2521 taaggatgat tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca
     2581 ctatttactt tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt
     2641 ctctattaaa aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc
     2701 gatttcttga attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc
     2761 ctgctgacga atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt
     2821 cactggtttt gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct
     2881 ttgctcaaat tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac
     2941 ggtcccttcc ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga
     3001 cagatattct gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc
     3061 aataggtgca ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa
     3121 catacgcgga ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg
     3181 ctttcgctgt ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat
     3241 tggaccaacg attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt
     3301 tgtgatgaga tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat
     3361 ttctttttgt gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt
     3421 ttttgtccag tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat
     3481 cagcgcatgc tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa
     3541 aggaatcttt ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag
     3601 tcgaggctca aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc
     3661 tttggtaaaa gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg
     3721 ttttttcgta aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg
     3781 agcaaaagtt ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc
     3841 aacctcaatc attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt
     3901 cttcatgaaa tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa
     3961 aaaacacagc gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt
     4021 tcacaacttc taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac
     4081 gcaacaattt tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt
     4141 aaaaataaac atgagaacat aaaa
//
LOCUS       DACCWK010000146         4020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCWK010000146 DACCWK010000000
VERSION     DACCWK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.152"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(132..464)
                     /locus_tag="IVW65_002522"
     CDS             complement(132..464)
                     /locus_tag="IVW65_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7901367.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(1301..2341)
                     /locus_tag="IVW65_002523"
     CDS             complement(1301..2341)
                     /locus_tag="IVW65_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7901368.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2690..3016)
                     /locus_tag="IVW65_002524"
     CDS             complement(2690..3016)
                     /locus_tag="IVW65_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901369.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(3003..3791)
                     /locus_tag="IVW65_002525"
     CDS             complement(3003..3791)
                     /locus_tag="IVW65_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7901370.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1219 a          682 c          578 g         1541 t
ORIGIN      
        1 ccccgaaggg aattgttgat agatcaagtc tactccttat ttttctagtg attctaagtt
       61 cctttagaag tctcgacaat attttcaagc aagcgaaaaa cgataaaata caagttatga
      121 gaatgatcaa ttcagctgat taaacgcccc atcccttttg cggtacaacg caagactcca
      181 ttttttcttc gaccaatcag tacaccgttt tggtcataaa catgtttgat tgttggatat
      241 agctctttta aagggatagg atcttttaaa attatttgtt tttgtttccc aatgtaataa
      301 atcatttctt ctgagacttt gtctttctgg attatatatt tcttacactg cacaacggtt
      361 ttactttgac cagttgatag tttcttcgtg atcttttctg tagcggtaca ccgtttaccg
      421 tagtgacaaa tatcttcgag actcaaaatt cctgtatatg acataaaata atccacccac
      481 ttactttaaa tatttttaac aagtttttct tgttattttt caaaaataga gaattgattt
      541 tcgttcatga caaacaaaaa agagggaacg cattcaaaca tgcgttccct ctaatttcag
      601 cgttcgtcat acggcaaaga gccccacgga catttttata acattatgct taggcataga
      661 ttcatcgtaa tatcacgtat ttttaacctt aacacagcca aaaaaagtgg tatcactttt
      721 gaatatctgt tatactaaat ccgttacggg taataccgta acactgaaag gtaaacgcat
      781 taaggggtat agacttaatg ccacctgtgg acgtcgacga aggttaccag ccttccgagt
      841 ctcacagggc ctttcttttt tttgtatttt caattgtcca aatttatcgt ccaaaagtgc
      901 agaacgaaaa tcaattcatc acaagcatag cagacaatta aatttaaaac aactaaaaat
      961 atacaaaaaa gtgtaattga atattagcag cactgcctga aaagaagtaa tatcaggctt
     1021 ttcagcttgg ggaaataagt gaattacctc tcttcatgaa ttattataac tcaaaaaagc
     1081 acatatgcaa acactagttc cctatttttt tgagtgaaaa ataactaaaa tatctaacga
     1141 ttatgaattt aaaacctacg attgtgattg ttttcataaa cttttaatct aaaaactccg
     1201 gaatactaac tatttatttg taatatttgt aataaaatta aaatcataaa aaagttctaa
     1261 tttaaaattg atcattttta tagtaattgc tttttatcaa ctactctaac caattttccg
     1321 tactaactgt tggtaattcg ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa
     1381 tagctttttc gacaaaccaa ttttttacat aaacgaaaat tagattctgc aaattgttta
     1441 ctttttcagt tttttgtttt tgataagctt tcagtaaagt catatataat cctttgtcag
     1501 catcaattcc ataagattcc agttcttcat aaacgacaat agtttgctct aattcttcag
     1561 cttttttctt tgcattatga atagtttgga cggtttgttg agcttcactg taacttgaag
     1621 aaaatgtttt tatcaagttt aatacatttt ctggtacaaa tgtatttata ctgtcctctt
     1681 gcatgatgtc gacgaagtta tctagcaaaa cttgattttg taactggtca gtttcagtgt
     1741 ctattatgtg tctattagtg tctatagaat tagaatagaa tagattgtct gcgattttcg
     1801 caattacggt tgagtccttg atatgactgg attttgccgt atttgcgatt ttcgcaatta
     1861 cggtagaatc attgattttt cgcggttttg ccgtatttgc gattttcgca attacggtgg
     1921 aatcagtatt gtcttcctct tccatgattt taaagacgtc ttgattcgta acttctggct
     1981 tgcctaagta caataaatta ggcatagatt caatcttacc atcacgttta tttactcttc
     2041 ctctcttctg tttcagaaga cctgcttttt ctaattcttt tttgatctta gatactttac
     2101 cttcgcggca ttgtaacaat ttcattaatt cttcaactgt gaatatgaag aatatattat
     2161 catcagaatc tacccagtta tttttgactg aataatcaaa tcgatcttta agtagcatat
     2221 aagcaatttt tgcatcattt gaaagatcca tgtatttttc gttagtaaaa aataccttag
     2281 gcatttgata atatttttca cgataaattt cttttgattt gtaataatta aaatcattca
     2341 tagccaatcc tctttctgta ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa
     2401 caaatatatc tatccactat atatttttct actgctgata agtcgccaaa ctttgttaaa
     2461 gcagtagtct ttttttatct ttttttagtt taccttaaaa aataaattaa agcaaaccgc
     2521 tctatttcag ggtttaagct catggtaact atatcacgat attacaataa cattatatca
     2581 cgatatcgta atattacaat atgaagataa gcgacttatc agctttgata ctactgattt
     2641 ttataaaaaa aatatcacga tattatgata tcgtgatatt gaatgtaaat taaattttat
     2701 tttgtttctt tgctagttta tatccttcaa tcaagtattc ttcaggtatg gattttaata
     2761 gcgcatcttc tataacgtct ggtttacttt tgtctgattc agcaatagtc gtccaatatt
     2821 tgagtgctga cattagaatt ggatttatac gaatgctatc tgctgttctt acactacttc
     2881 cttgatcttt tatgacttta cttatatttt tcttggttaa ttcgatttct tctacttctt
     2941 ttggtttgta gttcttttta tttttatttc ctagtagtct acttactgca gcttgactgc
     3001 gattatcttc tgccatcaaa atcaccaact tcttttaaat attcttctgt aaatgcttta
     3061 tagtcatctt cagctgttga gggtttatta aaattgatta gatctgatcc agcagcttgc
     3121 gcttcgtcaa cggttattgt tctacgaata taagttttga aaatttttgc accaatgact
     3181 ctagaaattt cggttgctgc agtcgtcatt tctttagaaa tgttataacg cttattatac
     3241 ttagtaaata ggactcctcg tacttcaagc ttgtcgttcg tatattcttt taccgtttca
     3301 atagtttcac ttaactttat tagtcctttt aatgagaaat acgcagcttc aactggaatg
     3361 ataatttcat cagaaacagt aaatgcgttg actgtaagca ttcctaaact tggtggacaa
     3421 tcaattataa tataatcata gttgtccttg attggttgaa gtactctttt aagcctgtgt
     3481 tcacttccta cttgagtaaa ttccctctct gcagaagaaa gggctatgtc ggctggcaaa
     3541 acatcaattc ctttatagtc cagaatagca tctgtaatgt cagcctcccc ttttaatact
     3601 tcataaattg taggttggtt tgttccatct atatcgaaac ctacattatc tgtagcattt
     3661 ccctgaggat caatgtccac taaaagtaca tttttaccgt aaacaaattt caaacatgct
     3721 gctaaattaa cgctagtcgt agttttagaa actccacctt tttgattggc tactgacaat
     3781 attcttgcca ttaaaatatc ccccattcat cttatattat aatataatta tatcataata
     3841 ttgtgatatt gcaatatcgt atatgcatta tattgtaata ttgtgtgatt attttgtttt
     3901 aaagatatat atcggttcgg atataaagat tattcatatg ttattcgaaa gtaaaataat
     3961 cactttttat tactttaaaa ttttttatta atcataagaa atatgttttg cgaggagagt
//
LOCUS       DACCWK010000147         3910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCWK010000147 DACCWK010000000
VERSION     DACCWK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.153"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            8..1381
                     /gene="tet(L)"
                     /locus_tag="IVW65_002526"
     CDS             8..1381
                     /gene="tet(L)"
                     /locus_tag="IVW65_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7901371.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            1945..3207
                     /locus_tag="IVW65_002527"
     CDS             1945..3207
                     /locus_tag="IVW65_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP7901372.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3452..>3910
                     /locus_tag="IVW65_002528"
     CDS             3452..>3910
                     /locus_tag="IVW65_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP7901373.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         1334 a          542 c          811 g         1223 t
ORIGIN      
        1 attaattatg aagtgtaatg aatgtaacag ggttaaatac aaccaaattt taatttggct
       61 ttgcgtttta tcttttttta gcgtattaaa tgaaatggtt ttgaacgtct cattacctga
      121 tattgcaaat gattttaata aaccacctgc gagtacaaac tgggtgaaca cagcctttat
      181 gttaaccttt tccattggaa cagctgtata tggaaagcta tctgatcaat taggcatcaa
      241 aaggttactc ctatttggaa ttataataaa ttgtttcggg tcggtaattg ggtttgttgg
      301 ccattctttc ttttccttac ttattatggc tcgttttatt caaggggctg gtgcagctgc
      361 atttccagca ctcgtaatgg ttgtagttgc gcgctatatt ccaaaggaaa ataggggtaa
      421 agcatttggt cttattggat cgatagtagc catgggagaa ggagtcggtc cagcgattgg
      481 tggaatgata gcccattata ttcattggtc ctatcttcta ctcattccta tgataacaat
      541 tatcactgtt ccgtttctta tgaaattatt aaagaaagaa gtaaggataa aaggtcattt
      601 tgatatcaaa ggaattatac taatgtctgt aggcattgta ttttttatgt tgtttacaac
      661 atcatatagc atttcttttc ttatcgttag cgtgctgtca ttcctgatat ttgtaaaaca
      721 tatcaggaaa gtaacagatc cttttgttga tcccggatta gggaaaaata taccttttat
      781 gattggagtt ctttgtgggg gaattatatt tggaacagta gcagggtttg tctctatggt
      841 tccttatatg atgaaagatg ttcaccagct aagtactgcc gaaatcggaa gtgtaattat
      901 tttccctgga acaatgagtg tcattatttt cggctacatt ggtgggatac ttgttgatag
      961 aagaggtcct ttatacgtgt taaacatcgg agttacattt ctttctgtta gctttttaac
     1021 tgcttccttt cttttagaaa caacatcatg gttcatgaca attataatcg tatttgtttt
     1081 aggtgggctt tcgttcacca aaacagttat atcaacaatt gtttcaagta gcttgaaaca
     1141 gcaggaagct ggtgctggaa tgagtttgct taactttacc agctttttat cagagggaac
     1201 aggtattgca attgtaggtg gtttattatc cataccctta cttgatcaaa ggttgttacc
     1261 tatggaagtt gatcagtcaa cttatctgta tagtaatttg ttattacttt tttcaggaat
     1321 cattgtcatt agttggctgg ttaccttgaa tgtatataaa cattctcaaa gggatttcta
     1381 aatcgttaag ggatcaactt tgggagagag ttcaaaattg atcctttttt tataacagga
     1441 attcaaatct ttttgttcca ttaaagggcg cgattgctga ataaaagata cgagagacct
     1501 ctcttgtatc ttttttattt tgagtggttt tgtccgttac actagaaaac cgaaagacaa
     1561 taaaaatttt attcttgctg agtctggctt tcggtaagct agacaaaacg gacaaaataa
     1621 aaattggcaa gggtttaaag gtggagattt tttgagtgat cttctcaaaa aatactacct
     1681 gtcccttgct gatttttaaa cgagcacgag agcaaaaccc ccctttgctg aggtggcaga
     1741 gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga aaggtcttaa aggttttatg
     1801 gttttggtcg gcactgccga cagcctcgca gagcacacac tttatgaata taaagtatag
     1861 tgtgttatac tttacttgga agtggttgcc ggaaagagcg aaaatgcctc acatttgtgc
     1921 cacctaaaaa ggagcgattt acatatgagt tatgcagttt gtagaatgca aaaagtgaaa
     1981 tcagctggac taaaaggcat gcaatttcat aatcaaagag agcgaaaaag tagaacgaat
     2041 gatgatattg accatgagcg aacacgtgaa aattatgatt tgaaaaatga taaaaatatt
     2101 gattacaacg aacgtgtcaa agaaattatt gaatcacaaa aaacaggtac aagaaaaacg
     2161 aggaaagatg ctgttcttgt aaatgagttg ctagtaacat ctgaccgaga tttttttgag
     2221 caactggatc caggagaaca aaaacgattt tttgaggaaa gttataaatt attttccgaa
     2281 cgatatggca agcaaaatat tgcttatgca acagttcata atgatgagca aacccctcac
     2341 atgcatttag gtgttgtgcc tatgcgtgat ggaaaactgc aaggaaaaaa tgtgtttaat
     2401 cgtcaagaac tgttatggct acaagataaa ttccccgagc atatgaaaaa acagggtttt
     2461 gagttgaagc gtggtgaacg tggctctgac cgtaaacata ttgagacagc taaatttaaa
     2521 aaacaaactt tggaaaaaga gattgatttt ctagaaaaaa atttagcagt taaaaaagat
     2581 gaatggactg cttatagcga taaagttaaa tcagatttag aagtaccagc gaaacgacac
     2641 atgaaaagtg ttgaagtgcc aacgggtgaa aagtccatgt ttggtttggg aaaagaaata
     2701 atgaaaacag aaaagaaacc aaccaaaaat gttgttatat cggagcgtga ttataaaaac
     2761 ttagtgactg ctgcgagaga taacgatagg ttaaaacagc atgttagaaa tctcatgagt
     2821 actgatatgg cgagagaata taaaaaatta agtaaagaac atgggcaagt taaagaaaaa
     2881 tatagtggtc ttgtagagcg atttaatgaa aatgtaaatg attataatga gttgcttgaa
     2941 gaaaacaagt ctttaaagtc taaaataagc gatttaaagc gtgatgtgag tttaatctat
     3001 gaaagcacta aggaattcct taaggaacgt acagacggct taaaagcctt taaaaacgtt
     3061 tttaaggggt ttgtagacaa ggtaaaggat aaaacagcac aattccaaga aaaacacgat
     3121 ttagaaccta aaaagaacga atttgaacta actcataacc gagaggtaaa aaaagaacga
     3181 agtcgagatc agggaatgag tttataaaat aaaaaaagca cctgaaaagg tgtctttttt
     3241 tgatggtttt gaacttgttc tttcttatct tgatacatat agaaataacg tcatttttat
     3301 tttagttgct gaaaggtgcg ttgaagtgtt ggtatgtatg tgttttaaag tattgaaaac
     3361 ccttaaaatt ggttgcacag aaaaacccca tctgttaaag ttataagtga ccaaacaaat
     3421 aactaaatag atgggggttt cttttaatat tatgtgtcct aatagtagca tttattcaga
     3481 tgaaaaatca agggttttag tggacaagac aaaaagtgga aaagtgagac catggagaga
     3541 aaagaaaatc gctaatgttg attactttga acttctgcat attcttgaat ttaaaaaggc
     3601 tgaaagagta aaagattgtg ctgaaatatt agagtataaa caaaatcgtg aaacaggcga
     3661 aagaaagttg tatcgagtgt ggttttgtaa atccaggctt tgtccaatgt gcaactggag
     3721 gagagcaatg aaacatggca ttcagtcaca aaaggttgtt gctgaagtta ttaaacaaaa
     3781 gccaacagtt cgttggttgt ttctcacatt aacagttaaa aatgtttatg atggcgaaga
     3841 attaaataag agtttgtcag atatggctca aggatttcgc cgaatgatgc aatataaaaa
     3901 aattaataaa
//
LOCUS       DACCWK010000148         3844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCWK010000148 DACCWK010000000
VERSION     DACCWK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.154"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..888
                     /locus_tag="IVW65_002529"
     CDS             <1..888
                     /locus_tag="IVW65_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7901374.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            1042..1896
                     /locus_tag="IVW65_002530"
     CDS             1042..1896
                     /locus_tag="IVW65_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7901375.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIVHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
     gene            1988..2203
                     /locus_tag="IVW65_002531"
     CDS             1988..2203
                     /locus_tag="IVW65_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7901376.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2221..2493
                     /locus_tag="IVW65_002532"
     CDS             2221..2493
                     /locus_tag="IVW65_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7901377.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            2495..3358
                     /locus_tag="IVW65_002533"
     CDS             2495..3358
                     /locus_tag="IVW65_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7901378.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1553 a          584 c          659 g         1048 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc ataaagaggg cttttatata ttaatcacaa atcacttatc acaaatcaca
      961 agtgattaat cacaaatcac ttgtgatttg tgattcttaa tgatacaata ttactataca
     1021 aaaaaagaat ggggcgtagt tatggagaag gaaaaactaa aaatacttga agaattaaga
     1081 cgtattttaa acaataaaaa tgaagcaatt attatcttga ataattactt taaaggtggt
     1141 gttggaaagt ccaaattatc gactatgttt gcttacttga cagacaaatt gaatttaaaa
     1201 gttttaatga tcgataagga cttacaggca acattgacaa aagacttagc aaaaactttt
     1261 gaggtagaat tgccacgtgt caatttttat gaaggcttga aaaatggaaa cttggcttct
     1321 tctattgttc atttgactga taatttagac ttgatccctg gcacgtttga tttgatgtta
     1381 ctgccaaaat taactcgctc atggactttt gaaaatgaaa gtagattgct tgctactctt
     1441 ttagcacctt taaaaagtga ctatgatctc attattattg atactgtacc aacgccaagc
     1501 gtttatacaa ataatgcaat cgtggcgagt gattacgtta tgatcccttt acaagcagaa
     1561 gaagaaagta caaacaacat tcaaaactat atttcctatt tgattgattt acaagaacaa
     1621 tttaaccctg gactagatat gatcggtttt gttccttatt tagttgatac ggacagcgca
     1681 acgataaaat caaacctgga agaactgtac aagcaacata aagaagataa cttggttttc
     1741 caaaatatta tcaagcgaag taataaagta agtacctggt ctaaaaatgg aattacagaa
     1801 cacaaaggct atgacaaaaa agttttatcc atgtatgaga acgtattttt tgaaatgctt
     1861 gagcgaatta ttcaattaga aaacgaaaaa gaatagaatc acaaatcaca agtgattaat
     1921 cacaaatcac ttgtgatttg tgattgttga tgataaaata agaataagaa gaaatagaaa
     1981 gaagtgagtg attgtgggaa atttaggcgc acaaaaagca aaacgaaatg atacaccaat
     2041 cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg acttacacca
     2101 tattataaaa attgaaacag caaaaaatgg cggaaacgta aaagaagtta tggatcaagc
     2161 cttagaagaa tatatacgga aatatttacc tgataaactt taaaaaggag tgaaaatgaa
     2221 atggcagtta cgtatgaaaa aacatttgaa atagagatca ttaacgaatt atcggcaagc
     2281 gtttataatc gagtattaaa ctatgttttg aaccatgaac tagatactaa aaatactcgt
     2341 ttactagaag tgaatctttt aaatcaatta gaagtggcac aagaagttga tttatttcaa
     2401 caaccatttg aagaattaca agctattcat gagtattggc ggtcaatgaa tcaatattca
     2461 aaacaaattt tgacaaaaga gaaagtggct taacatggcg aatattactg acttcaccga
     2521 aaagcaattt gaagatcgtt tagaaaagaa tgttgaacga ctaactaaaa atagactagc
     2581 ggttgaatcg ccaaccgctt ttttacttgg tgggcaacca ggatcaggta aaactagttt
     2641 gcgatcggca atttccgaag agacacaagg aaatgttgtt atcattgata atgatacgtt
     2701 caaacaacag caccctaatt ttgatgaact agtgaaactt tatgaaaaag acgtagtaaa
     2761 atacgttacc ccttattcta atcgcatgac agaagcgatc ataagccgtt tgagagataa
     2821 agggtataat ttagtgattg aaggtacagg acgaacaaca gacgttccta ttcaaaccgc
     2881 aacaatgctt caagccaaag attatgaaac aaaaatgtat gtcatggcag tacctaaaat
     2941 caactcatat ttaggaacaa ttgaacggta tgaaactatg tatgcagatg atccaatgac
     3001 agccagggca acaccaaaac aagcgcatga tattgttgtc aaaaacttac cgaccaattt
     3061 agaaaccctt cataaaacgg gcttatttag cgatataagg ctttacaata gagaaggagt
     3121 gaaactctat tcaagcttag aaacgccttc cattagtccg aaagagacct tagaaagaga
     3181 attaaatcgt aaagtatcag ggaaagaaat tcaaccgact ttggagagaa tagagcaaaa
     3241 aatggttcaa aatcaacacc aagaaacccc tgaatttaaa gcaattcaac aaaaaatgga
     3301 aagcttgcag ccacctacac caccaatacc caaaacacct aaacttccag ggatttaaac
     3361 cttaaaaaag taaaagagta gttaccaaaa aacggtaact actctttttt tataaaaaca
     3421 tttcctcatt ttttaaaagc tgatcgagaa ataaagcaca caactgtatt ctttcttttt
     3481 tcagttggta cattttgcag tctgtaacgt gccatttact gtttgttacc ttaacatatc
     3541 cagtcgaaaa cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttgtgtct
     3601 atgttgcgtt ctcgtcacag caaacgacac gaacacactg gcgaaaatga accaacaaaa
     3661 cgaaaaaaga aaggagcgta cactgtaatt tatttctagc ttttaaaaca tgaggaaaac
     3721 agcttcactt cctcaaacgc ctaacaaaaa tggttgtcaa aaaactaaaa atcaaaaaaa
     3781 gaaaagagga aaagaaaatg gaaaaaacaa aaaaattaca attagaagat tttacagaaa
     3841 atgg
//
LOCUS       DACCWK010000149         3820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCWK010000149 DACCWK010000000
VERSION     DACCWK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.155"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(8..421)
                     /locus_tag="IVW65_002534"
     CDS             complement(8..421)
                     /locus_tag="IVW65_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP7901379.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(692..1384)
                     /locus_tag="IVW65_002535"
     CDS             complement(692..1384)
                     /locus_tag="IVW65_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7901380.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRSGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1379..1645
                     /locus_tag="IVW65_002536"
     CDS             1379..1645
                     /locus_tag="IVW65_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7901381.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1821..2390)
                     /locus_tag="IVW65_002537"
     CDS             complement(1821..2390)
                     /locus_tag="IVW65_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7901382.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2464..2787)
                     /locus_tag="IVW65_002538"
     CDS             complement(2464..2787)
                     /locus_tag="IVW65_002538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901383.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2928..3752)
                     /locus_tag="IVW65_002539"
     CDS             complement(2928..3752)
                     /locus_tag="IVW65_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901384.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFLHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKYFTKNGFVT"
BASE COUNT         1107 a          721 c          632 g         1360 t
ORIGIN      
        1 taaattttta gttattaact tgattaaaat cagagataat atcatttaat tcagtttgaa
       61 gggttgtaac gattaaatgg aaattttctt catctgtttt attatatggg gatgttttcg
      121 ttatatataa caacttttct ttttcaattt tcattaataa ctctgtcgaa gtctgtactt
      181 tatcttgata attaaatgca gacatcccac tttgtataat tgtaattatt gctgaagcaa
      241 ttgaaacaat caatagtaaa gacctgtgtt cggaagcctg atcaattaat attgggatac
      301 tagctgataa gactattttt ataatatttc cgataccaat tactctatta taaaatcgaa
      361 tttctttttt taatctatcg attgtagaat caatagattc gataaacgct ttttgatcca
      421 tcattttctc tccaatcatt ttattaaaat cattaacaaa aatattataa taggatttaa
      481 gtaagttcta actaatcaca tataaaaata aaaaaacatt tgtattttat caaagtttta
      541 ttatacttgt caatttgtgt acatcctaaa atataagcta tttgtttact tctgtagaat
      601 atatactttg ttgttagata agttgcacat gtttatagaa tctaaataat aaatttttat
      661 gtaccgcccc tcaaagaggg ggggggattt tttatcgttg tggaatattt aaataccaac
      721 gcttgtcatg aaaatcttgt gctccaccct tagtatttcc ttctggatcg tttgtcgcac
      781 gcatcatgac gtatactttc ttattaggga aatcacgcat attgaaagat acatgatagc
      841 caacatttcc taaagtatta taagcttgat ttacgtctgg tctataaatc ccatcagctc
      901 ttactctagc tagttctttt ccagtattgt agtccataat gaaaatatac tcgtatttat
      961 aattagcaat gtgccatcca gctacatgca agtttgcgtt ttcgatttct ccgaactgat
     1021 caatgtgggc gtaatttgtt ccatctgtca gcgtagaatt tgcagcacct gatcgagttg
     1081 gatcaattac tggtttatcc tccgaagtag ttggattatc atccgtaaaa ccatgagcca
     1141 aatcataggc tagtttttct ttacttacac ccatttcaga aagataaccg taaggatctg
     1201 tatgatcgcc ccaaatattt tgtgttaccc ataaatgcga tttgattcct ggttggttat
     1261 aaggagtgtc taatgttaat ggaataccat atttcattgc tgaatctctt gccaattcaa
     1321 cgtatacttt atagttcttt tcaaaagttt ctttatcatg tgtgtgttgt aactcgattt
     1381 gcacaggact tttggctcac gaggttgtcc tactctacat cttgctgata gatatgctga
     1441 aaggaaaaca ccaacattat gttttgcttt ttcagctaga acgagacaag caggaatacc
     1501 tttagaagat atcaaagatt ttttaggtca taaagatgtt tccactacac aagtctatgc
     1561 ccatatttct cccgaagtga aaaaaagatc aatgaatcaa ctagaaaact atatagaaga
     1621 gcaaataaaa aagcactcaa attgagtgct tttttaagct cactatcact aaaactatca
     1681 ctaattttat ttataagcga acatgtttct atcactgatt ttttaaatct agcgaaaaac
     1741 ccttatctta aaggcgttcg ttcaattctt tggcaaggtc ttcaaagcct ggcttgccta
     1801 gaagtgcgaa catggttttc ttatagctca tgtagaaaaa ttttcttgat gatgatttta
     1861 tccatcaatt gagattgact gttttgctct cctatgattt cagctatgat tgtccgattt
     1921 tgcggttttc ttcttaactg gtagttgatt ttcccagttt ccatcaactc gctaagcata
     1981 tccacgactc cgccaaatcc ggtctttttc gtaataaaac ctagtgctac cattcgttca
     2041 tctagatttt ttgctgtaat cgtcacacca gtaatgagcg agtgctgtaa tgtttgggcc
     2101 agctcttcca tcccttcagc attggatacg acggaagctg ttttgttaat aggttcttcg
     2161 tttatttctt ttggttcttt cctctccact ttcactgttt gacgaaggac atctagttct
     2221 tgcaagagcg tttcgtattg ttcttgggtc agcatcacgc cagctacttt ttcacgatta
     2281 aatatatata cgccagttcc cgcgtctcgc gcttgagcaa atacatccat cggcgatcgt
     2341 ttcacttctg tgatcgaaga tgtgggaact tctaatttct ttaattccaa tggatttcct
     2401 cctttactaa tcgcttttta ttatagcaca gactgacttg ttttgatctt ttttccaaag
     2461 gttttatcgg tttttgccta accagtaatt gatatcggta agaagtcttt cgataccaaa
     2521 tgaaggatgg tacagatacg ttttttgatc ttcgttatgg tctagtggaa aaaaatgatc
     2581 ggtgatcact agatctgcat accgaggcgg cttatttcga gtacatacat gtatagagat
     2641 ccgttttcga tgtcttagcg ttttgttcag taccccagca agtcttcttg cttcttggtg
     2701 ttcatagcga aaaaaaaaga cgattttcac ttcaggaggg tggacatcta aataccctcg
     2761 ttcctggagt gttttcagta aaagcatccc ccactctact gcggggtgtg caagtgcttg
     2821 ttggatcgct agccgaaatt gatatagttt atctttggaa ttgggaaatt tttctaatgt
     2881 ttcctgataa taacgaactt cttctatcag ccggtcatgg ataccaatta cgtaacgaag
     2941 ccattcttgg taaagtactt tgtcagcttt gatttgcttt cgctctcaaa ttcgctattc
     3001 agccaaaaca gcgaatcaag cttttcagtc acaatcgtca atttccgata gtcttgtgtc
     3061 tcttctatcc gtttccaccg cttttcagta atctggttac agctcaagcg caaaattgct
     3121 agataatttt tttcttttat tggcaaaatc tgctcctctc taaatagcaa ttcaggaaac
     3181 aattcaaaga atagtgttcg actgtctcct aaaatctcga tttctgccgg atcctttctt
     3241 tgcttcaata attggatgaa catctcagaa aaggactgct ctccatcatc tgcaaaaaaa
     3301 tgcagaaaat ctttcaaaaa gcaaaacagc agatatctta cttttctttc tgaaccaaag
     3361 atctgtgctt ttttataaga aaaagaaagg tggtactcgc caaaccactt tctcaatcgc
     3421 tcttgcgtag aacgatcgta cttctgctcg taaggttcat tcaatagcag atttttcagt
     3481 aaacaaaacg ggggtgattg gatcagaaaa acaaacaaga gataaggttc gctatatcct
     3541 tgatcataaa ctaactgaac ggttttctta tgaagaatca gctgaatggg acagtcgctt
     3601 tccgccaaac gtttcagatg ccgatccact gttttaacat cgattttcag ttcttcagct
     3661 aaatcagctt ttcgccagat caaatctttc attagattga ttagtgaaaa taaattctgt
     3721 gcattttttg ataatagttt ttgccatctc atcgatcatt tctcacctga tttctaaaca
     3781 tattgtttga tcaaaatggg ttcactccta aaaagataaa
//
LOCUS       DACCWK010000150         3661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCWK010000150 DACCWK010000000
VERSION     DACCWK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.156"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..207
                     /locus_tag="IVW65_002540"
     CDS             <1..207
                     /locus_tag="IVW65_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7901385.1"
                     /translation="HITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRV
                     FKKYAGVSPKQYQLGLVDNFNNIS"
     gene            224..658
                     /locus_tag="IVW65_002541"
     CDS             224..658
                     /locus_tag="IVW65_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901386.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1275..>3661
                     /gene="esp"
                     /locus_tag="IVW65_002542"
     CDS             1275..>3661
                     /gene="esp"
                     /locus_tag="IVW65_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP7901387.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1335 a          522 c          707 g         1097 t
ORIGIN      
        1 catattacga tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg
       61 aaatcaaacc atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac
      121 ttttcgagag tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta
      181 gtggacaatt tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt
      241 acgtagtttg gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc
      301 tgatttgagt tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa
      361 tgaatatgac aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc
      421 tatcagtcat attaatggta gcaattttaa acaaacatta tttaatctga ttaagctaga
      481 tgctaaaata tcaagttatt tattcttttt gatacatgat gaatttatgg attatcaaaa
      541 attggatcag ttaattgaaa gagaaagctg ggaagattat tacgtgggtc taactttttc
      601 agaaaagtta aacaactaca agttaataga ttataagtat ttgagtgaaa gcaattaatt
      661 attcatgtag agtactagac actttaaata tggacaattc aattataaag aaaaagaatc
      721 aataatttaa tgaggctgtg acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa
      781 atgttgataa ctccaatcat tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa
      841 ccgatacttt ataattataa agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca
      901 cctttttact tatgtcacag tctcattcag taatgcggga taaggctcct tattccgtta
      961 tttgaatact ctgaagttta atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc
     1021 atagaaatga acttaatttc agaaagaata catacatatc tttttatatg ttttcagctg
     1081 ctgaatggtt aaaaagcaca aatatccgaa aaaaacatga agaaatgtac tttttttgtt
     1141 gtaatgttca tgaaattctc taagtaaatt agttaacata aggttagtaa gattcctatt
     1201 ctgtgaataa tacgtatcag atttatcttt tattcttact aggaaagtaa ttattaaggg
     1261 agttgatttg aataatggtt agcaagaata ataagagagt atttctagaa aaaacaaaga
     1321 aacgagtatt gaagtacagt atcaaaaaat taagtgttgg ggttgcatct gttttgattg
     1381 gtgttggtct tgtcttggga acaactgaac tagttaaagc acaagatgaa atttctccaa
     1441 gcagcccact tgaaacagcg ataagctccg tacaagtagg tgacaaagta gcttcgggga
     1501 atacttttca ggagaatcct ggttatacga aaaattataa cttttcggat ttacaattta
     1561 gtcctcaaga actaactgga gacacactaa aaggcaatac aattggattt gaagtttacg
     1621 gaaaacataa tattgcagca tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac
     1681 gattagctaa atatgtagaa aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta
     1741 gacggacctt tgtaagaatt aatgattcgc tgggtagacc tacaaatatt tggaaggtca
     1801 attatattcg tgcaagtgat ggattatttg ctggggcaga aacaaccgat acgcaaaccg
     1861 ctcctaatgg ggtaattacg tttgaaaaga gcctagatga aatttttaaa gagataggca
     1921 tagacaatct caagaccgat cgtttaatgt atcgaatcta cttggtaagt catcaagacg
     1981 atgataaaat tgtaccgggt attgatagta cgggctactt tttaacagat tcagatgatt
     2041 tctataatag cttagatgta tctgaaaata atcctgacca gtttaaacat ggatcagtaa
     2101 gtgcaaaata tgagaaacct aatactcaaa caaaagatgg ttcaggatcc acaggtgcta
     2161 atggagctat aatcttagat cataagttaa cgaaaaatta taatttctca tattcatctt
     2221 ccgcaaaggg cacgccttgg tatgctaatt acaaaattga tgaacgatta gttccatatg
     2281 tagcgggtat acagatgcac atggttcagg ctgacaaagt aacatatgat gtttcgtttg
     2341 aatccggaac aaaagtagct gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg
     2401 gcataggttc aataactaat aatgatttaa ctaaacttat cgattttgct aatgcaagtc
     2461 cacgtccagt cgttattcga tatgttttac aattgactaa gccattagat gaaatcttag
     2521 aagatatgaa agcaacagca caagttgaag aaaataaacc atttggtgaa gatttcatct
     2581 ttgattcttg gttgtcggat acgaataaaa aattaatcca gaacacttat ggaacaggtt
     2641 attattattt gcaagatatt gatggtgatg gaaaccctga cgataaagaa gagagcggag
     2701 acacgaatcc atatatcggg aaacctgaat tagaagaagt atatgatgtt gacacaacag
     2761 ttaaggggaa agtattcatc cacgagttag cgggaacagg tcacaaagcc caacttgttg
     2821 ataaagaagg tactgtatta gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc
     2881 caatttcaga tactgtagaa tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga
     2941 aagatgaatt aaaaatccaa atcgtttctc caggttttga taaaccagaa gaaggttcaa
     3001 ccgttattaa ggaatcacca aaagcggttg ataaacaaac cgtggtagtt ggatttaaac
     3061 cagatgctaa agaatcaatt cggaataata aaaacttacc tgaagatgca gagtattcat
     3121 ggaaaacaga gcctgatact tctaacgtta ctgatagtac gaaaggtatt gtaactgtta
     3181 agatcggaaa tcgaactttc gacgtggatg tagagtttgc tgtaaaagct tctcaagcta
     3241 tggaaaatga tgcaacatac gtacctataa caacaactcc agaaacgaca gttcaaagtg
     3301 gaaaacctac atttgataaa ccagatgttc ctttagctaa agatgccttt tcaatttcag
     3361 atgtctataa taagtacttc ggtaatgcaa gtgttgatgc aaacactggt attgttacat
     3421 tcactccagc taaaggtgta ggagaatcgg agccgattac tggaacaatt cctattaaaa
     3481 ttgtttacca agatggttct gtaggcacga ccgatttagc agtaactgta agtaaagata
     3541 tttatgaaaa tccaggagaa aacattcctg caggctacca caaagtaacc ttcaccgcag
     3601 gggaaggaac aagtattgaa agtggaacaa cagtctttgc agtgaaagac ggcgtaagct
     3661 t
//
LOCUS       DACCWK010000151         3566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCWK010000151 DACCWK010000000
VERSION     DACCWK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.157"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2303)
                     /locus_tag="IVW65_002543"
     CDS             complement(<1..2303)
                     /locus_tag="IVW65_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901388.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="IVW65_002544"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="IVW65_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1000 a          792 c          561 g         1213 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacat ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctataatat taccaaataa caaact
//
LOCUS       DACCWK010000152         3565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCWK010000152 DACCWK010000000
VERSION     DACCWK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.158"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            146..1912
                     /locus_tag="IVW65_002545"
     CDS             146..1912
                     /locus_tag="IVW65_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7901389.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1927..2364
                     /locus_tag="IVW65_002546"
     CDS             1927..2364
                     /locus_tag="IVW65_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7901390.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2377..3528
                     /locus_tag="IVW65_002547"
     CDS             2377..3528
                     /locus_tag="IVW65_002547"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7901391.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1193 a          635 c          771 g          966 t
ORIGIN      
        1 aattgaagtt ttctgatacc gaaatctttt tattttgctg acatactcaa acctttggca
       61 caaagtttcg tgccaaagta cgactatttc tctttttagt aagcggatac aattagagta
      121 tcaaaaggag agagggagag acaaaatgga acggacgatt cccaaaaaca acatttcgct
      181 aaaagcgagg gtacaaaaat taggcagtac tttatcaagc atggtcatgc caaatattgg
      241 agcactgatt gcctggggtg tattaacggc tctatttatc ccagatggtt atttaccaaa
      301 tgaggctttg gctaccatgg taagcccaat gctaacttat ctgattcctt tattgatcgg
      361 ttatactggc ggaaaagtaa ttgctggtga ccgcggatca gttgttgggg caattgcaac
      421 gatgggtgtg attgtcggaa cagatattcc aatgatgcta ggcgcgatga tcatgggacc
      481 tcttggtgga tatgccatca agaaatttga tcagctattc caaaaacgga tcaaatccgg
      541 ttttgaaatg ctagtcaata atttttcagc aggtcttatc ggttttggat tagcgttatt
      601 aggtttttct gcgattggtc cagttgtcga tgcattgact caagcaatgg caaaaggtgt
      661 agagatcatt ttatctgccc atttgattcc attgacaagt atttttattg aaccagcaaa
      721 aatcttattt ttgaataatg caatcaatca tgggattttg acaccattgg gaactgaaca
      781 agtagcagag gctggaaaat ctattctatt cttgcttgaa gcaaatcctg gtccaggact
      841 tggcgtatta cttgctttta ctttgtttgg caaaggagca gcaaaatctt cagcaccagg
      901 agccatgatc attcactttt taggcgggat ccatgaaatc tattttcctt acgttatgat
      961 gaaaccgtta ttattcttat cagttatagg cggaggaatg tcaggaagtt tcattttcca
     1021 attgatggat gctggactaa gagcgccagc ttctccaggg tcgatcattg cgattctagc
     1081 gatgacaccg ttcaattctt accttcctgt tattttgggc gtgattgttg ccgcagctgt
     1141 ttcgtttggt atttctgcag taattttgaa agcggatgcg aaagatacaa atgaagactt
     1201 cgaaaaatct gttcaagaga caaaggcagc taagcaagct tccaaaggtg cccaaggtat
     1261 tcctcaagga gctacattct ctggcgtaca aaaaatcatt tttgcttgtg atgcaggaat
     1321 gggatcaagt gcgatgggcg cttcgatctt gagaaaaaaa gtacaggaag cagacttgcc
     1381 tcaaacggtg accaatcaag caatcagcca actgacagat gactcgaata cattgatcgt
     1441 tactcaggtc gaactacaag aaagagcaaa acaaaaagca ccgagcgcgc gttttgtctc
     1501 agtagaaaac tttttgaatt cacctcgata tgatgaaatc attcaagaat taagcagcaa
     1561 gcagaaaaca tcagtttctg aaattgagaa tgagccagca gaagagtttc tttcagcaaa
     1621 catcaaacaa atcaaagaga ttctacttgt ccacgatgat cgttccggat cagcaacaat
     1681 ggggatgacc gtgctcaaag atatattaga caagaaccat ttaactatcc cgttaagaaa
     1741 agtcaatatc caagatttaa aagatgaacc tgattcactg atcgtagcca aagaaagttt
     1801 agcggaaaaa gtacagatgg ctgcacctca atccattcat ttatcagtca acagcatggt
     1861 caatccgcaa aaatacgaat cgattgcaca atatttgaaa cagacagctt aagggaggaa
     1921 aacagcatgc aaagtttgcc attcgacatg atcgagctca atcgatcttt taaaaccaaa
     1981 gaagaagcga tccgctattg cggtcagatg cttgtagaag cagggtgcgt tgatgaggaa
     2041 tatatcgatg ccatggtaga tagagacaaa atgttatctg tttatatggg gaattttatc
     2101 gcgattcctc atggaacaga tgaagcaaaa aaatatgtga aaaaatccgg catctgtgtc
     2161 gttcaagtac cagatggtgt agattttggt accgaagaag aagaaaagat tgcgactgtt
     2221 ttgttcggta ttgcaggagt cggagatgag catttgcaac ttgtacagca aatcgcactt
     2281 tattgcagtg atatggacaa tgttgtccag ctagcagacg cgctcagcaa agaagaagta
     2341 acggaaaatc tagcaattgc ttaaggagag agaaaaatga gagcagtaca ttttggtgca
     2401 ggaaatatcg gtcgaggatt tattggagag gttttggcag ctaatggatt tgagattact
     2461 ttcgtagatg tgaatcacac gatcattgat gctttaaatg aaagaaaaga atacgaaatc
     2521 gaattagcag atgaatcaaa agaaaaaatc actgtaaaaa atgtcaaagg aatcaacaat
     2581 caaacaaatc cagaaaaagt aatccaagaa atcgctgaag cagatttagt tactaccgcc
     2641 attggcccga atatcttacc ttttattgct gagttgattg caaaaggtat ccaagaaaga
     2701 gaaaaggaag gaagcacaac atctttagat atcattgcct gtgaaaatat gattggcggt
     2761 tcagcatttc tagaaaaaga agtgtataaa tatcttccgg aaacatcatt tactgacaaa
     2821 tatatcggct ttccaaatgc agcagtcgat cgaatcgtcc cattacaaca ccatgaagat
     2881 cctttattcg tccaagtgga acctttcaaa gaatgggtca ttgacgatag ccaaagaaaa
     2941 aataaaaaaa tccaattaaa aggggtttta tatgtagatg atctagaacc atatatcgaa
     3001 cgcaaactat ttagtgtcaa tactggtcat gcgactgtcg cttatacagg tgccttgtta
     3061 ggttaccaga ccattgatga agcaatgcaa gatgcgttag ttgtggcaca attaaaatca
     3121 gttttgcagg aaacaggaag tttgctgatt gccaaatggg ggtttgatgc agagcagcat
     3181 catgcgtata ttgaaaaaat cattcatcgc ttccaaaaca aaaatatctc tgatgcgatc
     3241 acacgtgtcg ctcggacacc attacgtaag ttaggttatc aagaaagatt tacacgtcca
     3301 gtgagagaac tacaagaaca taacttgact tgcccacatt tgacggcaac aatggggatt
     3361 atttttaatt actatgatcc tgaagatgaa caaagccgtc aattgcatga aatgaagatc
     3421 catgaaaact tggaacaatt gattcaagaa gtcacaggaa tcaatgatcc gaaaacaatc
     3481 gggaatatca agcaaaacgt gaaccgttat gccaagcaag tcgcttaaaa taggatacga
     3541 aaaaggggaa tgtgacgaaa ttttt
//
LOCUS       DACCWK010000153         3548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCWK010000153 DACCWK010000000
VERSION     DACCWK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.159"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(12..272)
                     /locus_tag="IVW65_002548"
     CDS             complement(12..272)
                     /locus_tag="IVW65_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901392.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            347..550
                     /locus_tag="IVW65_002549"
     CDS             347..550
                     /locus_tag="IVW65_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP7901393.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(691..1176)
                     /gene="ssb"
                     /locus_tag="IVW65_002550"
     CDS             complement(691..1176)
                     /gene="ssb"
                     /locus_tag="IVW65_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7901394.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1179..2078)
                     /locus_tag="IVW65_002551"
     CDS             complement(1179..2078)
                     /locus_tag="IVW65_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7901395.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2091..2684)
                     /locus_tag="IVW65_002552"
     CDS             complement(2091..2684)
                     /locus_tag="IVW65_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP7901396.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2925..>3548)
                     /locus_tag="IVW65_002553"
     CDS             complement(2925..>3548)
                     /locus_tag="IVW65_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP7901397.1"
                     /translation="NIIEILKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMT
                     AKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQ
                     EAARHLGICPKCQEQEVILYQKVAACTSEVCDFKLWTTIAKKKLTATQLKEIIQNSRT
                     SQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
BASE COUNT          925 a          701 c          588 g         1334 t
ORIGIN      
        1 cagctcttct tttatatgcc catcgatcgc gtaaaatgat cggggtttct aatttgcatc
       61 gattcaatga ctaattgatt ccgtttattg aaatgacctg tgacggcaat attgtatttt
      121 ccatcatcca taattaacaa tttatcgata atttcaatat ctgcaactac acaattaatc
      181 gattcatgaa ttgtttgtaa agtaaagcga accaataatg gttgtgtttt tattactcgg
      241 attttatcaa gcaaaccaac atatttgttc atcacgatca cctcgctaaa attatagcaa
      301 aaacagctaa aattgagaat ttttattcga ttttagatat gctataatga tcaagacagt
      361 aaagtttgtg acggtggcta tccgattgaa agtgaggtgg tgcgtatgtg cgtacttcca
      421 aaatcttaca gaaaggagaa gccttttgcc tgttgaaacg gcgctaggat taatgattag
      481 tttcggttct ttgatcgcaa caatcatttt tggaatctta gcagttgtaa acaacaatca
      541 aaaaaaataa ccgtcagctt tagccggtcg acgattattt ttcaaaaata atattaagct
      601 ggtcaccgtc ttaaacggtg ctgttagggg agttgttagc gcaactcctc ttttcgtatc
      661 cattataaca actttattga tcttttacaa tcagaatggt aaatcatcat cactaatatc
      721 gattgattgt ccgccaaatg gattttgtga agcgttcaat ccattgttgt taggcgtagc
      781 tacatcccgt tgttcaaagg tggtagggct tggactgctg gacgattgat cagcgtgcgc
      841 acggctttct gtcgctgctt tactttctaa tagttggaaa ttatcggcca caacttctgt
      901 cacatacaca cgttgtcctt gttggttatc gtaggttcgg gtttgaatcc gtccaacaac
      961 gccgatcaaa ctgccctttt tagctaatgt cgccatcgtt tctgctggtt tgcgccaaat
     1021 cacacagtta ataaaatcag cttctcgatt gccgttttga ttcgtgaaat ttcggttgac
     1081 agctagggta aaagtcgcta ctgcagtacc actcgcggta taacgtaaat ctggatcttt
     1141 ggttaagcgt cctactaaag ttacattatt gatcatcttt atctctccat ttcttgattc
     1201 atttgttgtg atcttttaaa ctgccgtttt ctttcttgat tttcaatttt gatcacactt
     1261 ttcattaggt gatcaatttc gttgactgta tctgtaaatg aaatggaatt cgcaaacgtg
     1321 ttaactgagc gatatttttc ccactgcttt tccaaatagt catttcctct gatcttatcc
     1381 atttctcttt cataataaaa ggacaacggt tttgtatccc cacgttttgt aaatgtattt
     1441 cgaacacttt tgtataactc tttcgtatcg atattttcta gtttcggaat ggtaaataag
     1501 tcgtagtaat cttttgctct cgaattatct gtgccaaagc tgaacgtagc acttagcttt
     1561 tctgcaataa tttgttctgt tggataagct ggaatctcca cttttttatc ttcaaacatt
     1621 aattggtgat agtgtgtatc tacttcagga taaatagaat ctcctgttgt cacatccatt
     1681 ttaaacggta cacgtacttt ctctaaatta gccaccaatt taatttgaaa tccaggataa
     1741 aaatcggctt ctcttgtttc cttcattcct tgaatagaga aaacaatgcc ttcttttgtg
     1801 ggttttctag ttaaatcatt aaaaatgctc ttcaattttt cttcagttaa tttgctattt
     1861 ctaaatgtcg tatcgatatc aattgttgtc cttctatcga ttccatattt tgatcctaat
     1921 aaaaagccgc ctttcaaaat gaagtcattt ttgtactttg attcagaaat ttgttcaatt
     1981 agacgatcca tcataaaatg ccgttgcact aactgtgcat ttaatcctgt actctttgct
     2041 aaatttcttg tttttgctgc aaattgtgtc ggtgtcatct ttttcctcca tcaatataag
     2101 ctttgcatgt ggtatttcat ttctttttct acatgtagtt ttgtcatgta atctcttaat
     2161 ttacttggat ctctcaatgg gtcttccatg tactctttta atgcttctaa tttcatttca
     2221 tattcaaatt catatctcgg attccaaata tcacaaagtg tacgctcttt atcatacata
     2281 gtaataggat ttccttgaaa ggaagttccc tctacttttc ctagttcata aacttctttc
     2341 ttcacgaaat gaaattgtaa ctgctgctct tttgtaattg aacgatcaac gttatagcca
     2401 tggggaaaag ttaagtgaat gaatttcggc atattcgtag aaagctggta aatatctaaa
     2461 gtcgtttcat gagagaagat tcccctggaa aatcgttgct gaagccaaaa taaatcatct
     2521 attccatgac catcctctac ataataacca cgatcaactt tgatcaattt tccttgattc
     2581 accaatcttt gtacagtggt tgctgcgatt ccaaatttcc tggcttcctt catagataaa
     2641 tttccatcgc gaatagccaa caattctttc agtttctctt gcatttactt cccctccctt
     2701 cgttacaaat acactacatc aaatatatca tagtagttta tttgtaacaa ggttaatgtc
     2761 tatcgatcta attcgatttc tttttgaatc ctctgctggt tcttcaattg ctgcagtctt
     2821 cgtttgctgg cacgcatttt ttcctgttct gaacgaattt tggctttttg attttcgatt
     2881 tcttgttgtt tgtgtgtggt attacgcata agattaaccc tccattattt tgtagtgcgt
     2941 cgtgttcttt tcttgaagtt cgtttttttc ttttcagaaa attcaaagcc tgtagtgaag
     3001 tcttctttta aaacaatcgt tgcttcaaac gaacccttct gaccttttaa ccctttcact
     3061 ggttggcttg ttcggctatt ttgaataatc tccttcagct gtgtcgctgt gagtttcttt
     3121 ttggcaatcg ttgtccataa tttaaaatca cagacttcag acgtacatgc tgccaccttt
     3181 tgataaagta tgacttcttg ttcctggcat tttggacaaa ttcctaaatg acgcgcggct
     3241 tcttgctttt cactggctgc tttcacttct tgtaacgtcg atccaaaatc agtggattgg
     3301 atttgccctg gtacgacttc caataagtga gagacaaatt tttggatatt agctagaaaa
     3361 gtggtttgtg ttccttttcg ttctccaatc tttaataagt agctttccca tttggcggtc
     3421 atttcggcgc tcgtaagcag cggctctttt tccacggctt ggcaaagcaa aatcccttta
     3481 ttcgtcacca cgagcttatt tttctctacc ttgatatatt ccttttgttt caagatttca
     3541 ataatatt
//
LOCUS       DACCWK010000154         3488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCWK010000154 DACCWK010000000
VERSION     DACCWK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.160"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..624
                     /locus_tag="IVW65_002554"
     CDS             <1..624
                     /locus_tag="IVW65_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP7901398.1"
                     /translation="NIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMT
                     AKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGKIQSTDFGSTLQKVKAASEKQ
                     EAARHLGICPKCQEQEVLLYQKVAACMSEACDFKLWTTIAKKKLTATQLKELIQNGRT
                     SQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            863..1456
                     /locus_tag="IVW65_002555"
     CDS             863..1456
                     /locus_tag="IVW65_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7901399.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRDTNKLRRYMDILKSPKEMTSYMLPLY"
     gene            1472..2371
                     /locus_tag="IVW65_002556"
     CDS             1472..2371
                     /locus_tag="IVW65_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7901400.1"
                     /translation="MTPTQFKAKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFRHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EKEKAERKQNFKRSQQMNQEMER"
     gene            2374..2847
                     /gene="ssb"
                     /locus_tag="IVW65_002557"
     CDS             2374..2847
                     /gene="ssb"
                     /locus_tag="IVW65_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7901401.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTTVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSTTSPSTTAFEQRDTNNGLDASQNPFGGQSMDISNDDLPF"
     gene            3213..3473
                     /locus_tag="IVW65_002558"
     CDS             3213..3473
                     /locus_tag="IVW65_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901402.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         1295 a          574 c          686 g          933 t
ORIGIN      
        1 aatattattg aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa taagctcgta
       61 gtgacgaata aaggaatttt gctttgccaa gccgtggaaa aagagccgct gcttacgagc
      121 gccgaaatga ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca
      181 caaaccactt ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtacca
      241 gggaaaatcc aatccactga ttttggatcg acgttacaaa aagtgaaagc agccagtgaa
      301 aagcaagaag ccgcgcgtca tttagggatt tgtccaaaat gccaggaaca agaagtctta
      361 ctctatcaaa aggtggcggc ttgtatgtct gaagcctgtg attttaaatt atggacaacg
      421 attgccaaaa agaaactcac agcgacacag ctgaaggaac tcattcaaaa tggccgaaca
      481 agccagccag tgaaagggtt aaaaggacaa aagggttcgt ttgaagcaac gattgtttta
      541 aaagaagact tcacaacggg ctttgaattt tctgaaaaga aaaaaacgaa ctttaagaaa
      601 agaacacgcc gcacgacaaa ataatggagg gtttatctta tgcgaaatac aacacacaaa
      661 caacaagaaa tcgaaaatca aaaagccaaa attcgttcag aacaagagaa attacgtgcc
      721 aacaaacgaa gactgcagca attaaagaac caacagcgga ttcaaaaaga aatcgaatta
      781 gatcgataaa ttcctttgtt gttaatataa aactaacgtg ttatagttat gttagtttta
      841 tattaacttt aggagaagag taatgaaaga caaaatccaa tgtatttttg ataaacataa
      901 tggcaaccta tccttgaagg atgctagaag tgaaggatta tcgcctatga cgttagatcg
      961 attagccgaa aaggataaaa tagaaaaggt agcaccaggt ttctatacct taaaggatgc
     1021 gttcgtggat gaattgtatc tcgctcagag tatctattcc agagggattt tttctcatga
     1081 gactgcactc gatctttttc aagtaggaac atatatccct aaaaagatca atatgatgtt
     1141 tcctaaagga tacaatgtat caaaggagaa attagatcag tatgccattc aaccgcatta
     1201 tacgaataaa gaaaattttg acctgggagt cacagaaaca gaaagctttt acggcaataa
     1261 aattttagtc tatgatttag agcgaacgtt atgtgatatg tggaatccaa gatataaagc
     1321 aagtgtagaa gtaaaacaag aagcattaaa agaatatatg acaagtacta gcagagatac
     1381 aaataagcta agaagataca tggatatttt aaaatcacca aaagaaatga cttcgtatat
     1441 gttgccactt tattgagagg agtaaaggtg aatgacgccg acacaattta aagcgaaaac
     1501 aagaaatttc gcagcagaga aacatttaaa cccacaaatt gtccaaagat attatatgat
     1561 ggaaaaatta ttagaatgta tcgttgaatc gccttatcat gacgattttg tattaaaagg
     1621 gggatttctg attggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac
     1681 aacttatcga aattcgaaac taacaaaaga aaagttagaa actgtttttt ctgaattaac
     1741 agctgatcct acaaaagatg gcattcgttt tgaattaaag ggattaacag agacgaggga
     1801 agccgattat tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa atgcacgtgt
     1861 accttttaaa ttagatatta cttcaggtga ttctattatt ccagcagcat tgatgtatga
     1921 acacaaatta atgtttgaag ataaaacgat tcatattcca gcttatccta cggaacaaat
     1981 tctagctgaa aaattacatg ccacgtttag ttttggaaaa gataattcgc gcatgaaaga
     2041 tttttatgac ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa atttgtataa
     2101 aagtgttcaa ggaacattca aggaaagaaa taatcaatcg gtgtttaggc atttgtatga
     2161 ccaggaaatg cctcaattgg tgaaagatga gggtttgaga gaaaagtgga aaagatacca
     2221 acaagaaaac tattttgcca aagacatttc tttcgaatct acactaactt ccgttgattc
     2281 cctgatgcaa tcaattgtag agaaagagaa ggcagaacga aaacaaaatt ttaagagatc
     2341 acaacaaatg aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtagga
     2401 cgattaacca aagatcccga cttgcgttat accgcaagtg gcacaacagt ggcgactttt
     2461 accctagctg ttaaccgtaa tttcacgaat caaaacggca atcgagaagc cgattttatt
     2521 aactgcgtga tttggcgcaa accagcagaa acaatggcga cattagctaa aaaaggtagc
     2581 ttgattggtg tagttgggcg gattcaaacg cggacttatg acaaccagca aggacaacga
     2641 gtgtacgtaa cagaagttgt ggccgataac ttccaactat tagaaagtaa agcagcgaca
     2701 gaaagccgta tgcacgctga tcaatcaacc actagtccaa gcaccaccgc ctttgaacaa
     2761 cgggatacaa acaatggatt agacgcttcg caaaatccat ttggtggaca atcaatggat
     2821 attagcaatg atgatctacc gttttaatta gcattttcta ttggttgcat ttgctacggt
     2881 ttatgttata atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg
     2941 gcgggctagt ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc
     3001 tttcttggca aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat
     3061 gattgcaaaa acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat
     3121 taggccgggg tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg
     3181 atttttgcta taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata
     3241 aaatccgagt aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg
     3301 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg
     3361 atggaaaata caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg
     3421 ctagcatgta tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaataaaag
     3481 aagagttg
//
LOCUS       DACCWK010000155         3377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCWK010000155 DACCWK010000000
VERSION     DACCWK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.161"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..205
                     /locus_tag="IVW65_002559"
     CDS             <1..205
                     /locus_tag="IVW65_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901403.1"
                     /translation="ARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKL
                     YLQSYKSAGNNWIATYSGTLTRK"
     gene            215..418
                     /locus_tag="IVW65_002560"
     CDS             215..418
                     /locus_tag="IVW65_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901404.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            629..1213
                     /locus_tag="IVW65_002561"
     CDS             629..1213
                     /locus_tag="IVW65_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901405.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1584..2909
                     /locus_tag="IVW65_002562"
     CDS             1584..2909
                     /locus_tag="IVW65_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7901406.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2902..3252
                     /locus_tag="IVW65_002563"
     CDS             2902..3252
                     /locus_tag="IVW65_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901407.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         1200 a          504 c          677 g          996 t
ORIGIN      
        1 tgctcgagca gcttctaaaa cgatacgagt gacaggaacg atgatttttg ataaagttgg
       61 ttggccttct agacctcctc aatcacggaa ttggaaagaa aaaagaaatg gttattggta
      121 ctatggaaaa ttgtatttac aaagttataa atcagctgga aacaattgga ttgcgactta
      181 ttcaggaacc ttaactagaa agtagggatt ttttatgcca gaatttgtga acacaccaaa
      241 tttttatcaa gatagcgata ttctaagtaa aattaattta ccgaaagaag aatcagcttt
      301 acaagttcag cgaactttac aggagacaca aaaaattcaa aaatcaattt ctaatctaga
      361 agattctgaa gaaaaaagaa aaaaaagaga ccaaaaaaca tatcaaaaag aaaaataatg
      421 attttaaaaa aagagtgtct agtcgacatt ctttttttct aattgaaagt gttcataaaa
      481 tcacccattt ctggacactt cctttttttg ctttattttt caaaaaaaca attcatttca
      541 tgttcataaa cccataacaa tatctatgtt cagttaaact atatggataa agttatgtta
      601 caataaaatt aatgaaggag tgattttttt gaaaatagga tacgcccggg tatctactgg
      661 tttacaaaat ttggatttac aagaagacag cctaaataaa tttggttgtg aaaaaatttt
      721 tactgatcat atgagtgggg caaaaagtaa tcgaccagga ttagaaatgg ccattgattt
      781 tgttcgctct ggcgatacac ttgtcgtttg gcgcttagat cgtttaggta gaaatatgga
      841 agatttgatt tcaatcgtca atcgactgaa tgagcgtggt gtgagttttc atagtattca
      901 agaaaatatt acgatggaca aatcaagttc tacaggacag ctcatgtttc atttgtttgc
      961 ggcatttgca gaatttgagc gtaatttgat cctggaacga tccgcagcag gacgagcagc
     1021 tgctcgtgca agaggacggt ttggtggtcg tcctgaaaaa ttgtctgaac aagatttgga
     1081 attactgaag actttagtgg atagcggtac acctattaag actattgcgg aacggtggaa
     1141 cgtctcaaga accaccattt atcgttattt ggcaaaaatg gaggagaaaa atggaaagac
     1201 aaattctcat tagtttttgt catttaatgc tgaaaatttt tgccatttat ctagaaaagt
     1261 gacaatagta tgagaatttt ttgtaagtgt ctactgataa cttattatca aatgttgtaa
     1321 ggattttatt catttgttaa tattaaagaa cggaactcca aaaaagtccg tactctaatt
     1381 tgcagtctct cactctttta gagtgagagt ttttattaga ttttcaaatt aattagtaaa
     1441 gtgatagaat aaattgatca tacaaatcat aagaagaagc agggtgatag catacctatg
     1501 cttctttttt tatgtattta gctatttctt ctttacaaca tcgaacatat gtttgtataa
     1561 tgtggaagaa tggagtgtta tttatgatgg attattcaaa agagccagta aatgattact
     1621 ttttaatcga tatgaaaagc ttttacgcaa gtgtcgaatg tatagaaaga aatttagatc
     1681 cattaacaac agaacttgtt gttatgagtc gatctgataa tactggttca ggattgatat
     1741 tagcttcttc tcctgaagca aaaaagcggt atggtattac aaatgtgagt agaccacgtg
     1801 acttaccaca accatttcct aaaacactac acgttgttcc accacgtatg aacctatata
     1861 tcaagcgaaa tatgcaggta aataatattt tcagaagata tgtggctgat gaagatctac
     1921 tgatttactc gatcgatgaa tcgattttaa aagtgacaaa atcactgaat ctttttacga
     1981 ctgaagaaac acgaagtcag cgaaggaaga agctcgctca aatgatccaa gaacgtatta
     2041 aagaagagtt aggtttgatt gctacggtag gcgtcggcga taatcccctt ttggctaaat
     2101 tagccttaga caatgaagca aagcataatg aaggatttat tgcggaatgg acgtacgaaa
     2161 acgttcctga gaaagtatgg aatattcccg aaatgacgga cttttgggga attggttctc
     2221 gaatgaaaaa aaggctcaac caaatgggaa ttttgagcat tagagacctt gctaactgga
     2281 atccttatac gattaagaat agattaggtg tgattggatt acagctgtac ttccatgcta
     2341 acggaatcga tcgaacagat atagcaatcc cgccagaacc gacaaaggaa aaatcttatg
     2401 gtaattcaca agtgttacca cgagattata ctagaagaaa tgaaatcgag ctggtggtaa
     2461 aagaaatggc ggaacaagta gccattcgta tcaggcagca taattgtaaa acaggatgtg
     2521 ttcatttaaa cattggtacg tcgatcttag aaacgagacc aggattttca caccagatga
     2581 aaatacctat cacagataat acaaaggaat tgcagaatta ttgtcttttc ttgtttgata
     2641 agtattatga aggacaagaa gtaaggcacg ttggaataac ctatagcaag cttgtgtaca
     2701 ccgattcgct tcagctggat ttatttagtg atccacaaaa gcaaattaac gaagaaaatt
     2761 tagataaaat tattgacaag attcggcaaa aatatggttt tacgtcgatt gtacatgctt
     2821 cttcaatgtt agaaagcgct agaagcatta ctagaagcac acttgttggt ggtcatgcgg
     2881 gcggaaatgg gggaattaaa aatgattgat tacaatgatc gtggacgttt aaagtgggca
     2941 gggttttatt tatctgatca tactgaagaa attgatgccg acaatgaaca acgaaccagt
     3001 caaaatttag caaaagaaca aatgacgact gaagagattg cggaagtatt gaacgctgca
     3061 gtcataaaaa atagatcagt atctattcaa aaagaagaac gaaacgctga aggatactat
     3121 ccagaagata ttgttggcaa gatcttaggt agtgatgagc tggggatcta cgttggaaaa
     3181 gacaaaattc actacgacga aatacgtcat atcgaatttt accaggataa taaatggagt
     3241 cgattagaat gaacggttct gttgcaaagt tttaaataaa gaataaaatc ccttacggta
     3301 tctatgattt aagctaggat tcccaataat accttgattt cagtacagac cgaaaacccg
     3361 aagagagtgc cttcttt
//
LOCUS       DACCWK010000156         3246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCWK010000156 DACCWK010000000
VERSION     DACCWK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.162"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(451..1761)
                     /locus_tag="IVW65_002564"
     CDS             complement(451..1761)
                     /locus_tag="IVW65_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7901408.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="IVW65_002565"
     CDS             complement(1773..3131)
                     /locus_tag="IVW65_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7901409.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
BASE COUNT         1012 a          660 c          506 g         1068 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataac
//
LOCUS       DACCWK010000157         3211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCWK010000157 DACCWK010000000
VERSION     DACCWK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.163"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..821
                     /locus_tag="IVW65_002566"
     CDS             <1..821
                     /locus_tag="IVW65_002566"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7901410.1"
                     /translation="TMVDSNGKPILNKYGKPKKVVKMPIDNSVTLSIQRLRHLASLGN
                     ESNKQIVDRYFSNQPEPLTINEQMELLQTGINLHSIYMVDENENEIITAPAIARWVIN
                     KHTLEVTARINPEYLEWFSEVVNQYILFNLRDFVKLKSKYSMILFLELSTFWNTGMYY
                     KRINDLREILGVNPTRNNRDFINNVLKPAIAALKEYFPELSLTVEKNSSDSRKITHVR
                     LKFDKLTITDMQERLRSSEGASLVEYQQELENMEGEEKAVNDPTPEIDLSNVEW"
     gene            935..1303
                     /locus_tag="IVW65_002567"
     CDS             935..1303
                     /locus_tag="IVW65_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901411.1"
                     /translation="MENTNQTPNTAPTNQTSPAERKADLIRLYSRLEGMKLQHVPTAP
                     KAPDGFPKSAESLEFFECKVCGSTFQTILRESVASGCPKCEHDRLGILADHLPEPDKN
                     SCLNNRKKPVTVMAMGFLCI"
     gene            1477..1785
                     /locus_tag="IVW65_002568"
     CDS             1477..1785
                     /locus_tag="IVW65_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901412.1"
                     /translation="MKEMKMKQVIYPVIIGVLAIALIILGVNCYNLSKENNQLETKNN
                     KQEIQIAKQAYVISDYKTYFRENYVDTVKYRSHFTDKQILNVYREALRDGKISDEGAN
                     "
     gene            2048..2368
                     /locus_tag="IVW65_002569"
     CDS             2048..2368
                     /locus_tag="IVW65_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901413.1"
                     /translation="MERFLTKNRKGGGKLEKKEDLLNAFKNLDEKDQLKALLRLHDIT
                     YSDIAERLTVKKGEREGQKGVSLVTVQKAFQSKTNLESDRWKEIVKVATKLIEERQND
                     QNKA"
     gene            2499..2708
                     /locus_tag="IVW65_002570"
     CDS             2499..2708
                     /locus_tag="IVW65_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901414.1"
                     /translation="MIRKLNNIKLSLAILTLFFGIYNNSRMALFFLTLLIFMNLIIHN
                     FKELENTAPAPSQGNQESEGMPHAW"
     gene            2698..2970
                     /locus_tag="IVW65_002571"
     CDS             2698..2970
                     /locus_tag="IVW65_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901415.1"
                     /translation="MLGSFKKDKEVKTRGALTPSQQNKLGTKATFHPVRDQANQKELI
                     GVCVGFTSEKFFPIIECKIHGITTREVARKVVKWHTSQTKTNHKKR"
     gene            3018..>3211
                     /locus_tag="IVW65_002572"
     CDS             3018..>3211
                     /locus_tag="IVW65_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901416.1"
                     /translation="MNNKNTKNTNTNHEKRSFFDKLFKKKKNGKVSILKMIGWIFLAF
                     FIVAVNSSGGDKKEAASKDTT"
BASE COUNT         1212 a          577 c          647 g          775 t
ORIGIN      
        1 taactatggt agatagtaac ggcaagccta tcttaaacaa atatggaaag ccgaaaaagg
       61 tagtaaaaat gccgatagat aactcggtta ctttgagtat tcagcgccta cggcacctag
      121 caagcttagg aaacgaaagc aacaaacaaa tagttgatcg ttacttttca aaccagccag
      181 aacctctgac aattaacgag caaatggaat tactccaaac ggggataaat ttacactcta
      241 tttatatggt agatgaaaat gaaaatgaga tcataaccgc gcccgctata gcaaggtggg
      301 taataaataa gcatacgcta gaagtaacag cgcggatcaa tccagaatat ttagagtggt
      361 tttcagaagt ggttaaccaa tacattttat ttaatctaag agactttgtg aagttaaaaa
      421 gtaagtactc catgatcctt ttcttagagc tttctacctt ttggaataca ggaatgtatt
      481 ataaaaggat taatgattta agagaaattt taggagtcaa tccaacgaga aataacagag
      541 attttataaa caacgttcta aagcctgcta tcgctgctct caaagagtac tttccagagc
      601 taagcctaac agtggaaaag aattctagcg actccagaaa gatcactcat gtaagattga
      661 agtttgataa gctaacaata accgatatgc aagaacgcct aagaagcagc gagggagctt
      721 ctttagtaga gtaccaacaa gagttggaaa atatggaagg agaggaaaaa gctgtcaacg
      781 atccaactcc agagatcgac ctttccaacg tggaatggta acacctgtca gcaatctaat
      841 ccaataaacc aaagtacttg taaggcgcga gggctccttt tagagccttc tagtcctata
      901 ccaaaccaag caaaataaag gagctataac caacatggaa aacactaacc aaacaccaaa
      961 tacagcacca actaaccaaa ctagcccagc cgaacgcaaa gccgatctta ttagattgta
     1021 ctcacgcctt gaaggtatga aactccaaca cgttccgacc gctccaaagg cgccagacgg
     1081 cttcccaaaa tcggcagaga gccttgagtt tttcgagtgt aaggtgtgcg gatcgacttt
     1141 tcaaacgatt ttaagggaat ccgttgcgag tggctgcccc aaatgtgaac atgatcgctt
     1201 aggaatttta gccgatcatt taccagaacc agacaaaaac agttgtttaa ataatcgaaa
     1261 aaaacctgta accgttatgg ctatggggtt tttgtgtatt tgagataatt tccaagggtt
     1321 atttatggat ttattttaaa aaatctatta aattatacta ccactggtag taatataaat
     1381 atatcgaaag ggtacgaggg ttaaagcgta atggcggaat gagtaccgtt ctcttttgaa
     1441 aataccaaac caaccaatta aaggagagaa agtaatatga aagaaatgaa aatgaagcaa
     1501 gtaatttatc ccgtgattat tggagtttta gctatcgctc ttattatcct tggtgtaaat
     1561 tgctataact taagcaagga aaataatcaa cttgagacca aaaacaacaa gcaagaaatc
     1621 caaatagcca agcaagctta cgttatcagc gattacaaga cttattttcg tgaaaactat
     1681 gtagacaccg tgaaatatcg ctcccacttc actgataaac aaattttaaa tgtttatcga
     1741 gaagctctta gagatggaaa aatcagcgat gagggagcta actagtggaa gagctcattt
     1801 tgctggctat gttattacag gaaaagaagc gtgaacgaga agcctaacag ctggaaagaa
     1861 tccgaatagg atatgaggaa gcagatcggg aaagagatca gaaaatagag gagctggaga
     1921 agcgtatcca agaactttcc aaaaactatg ataataacca agattgggaa caagagtggc
     1981 ggaaaatcgt tggtataaag gaaaatgaat acttagtgag aaagccgcca gaagaagagg
     2041 aagacccttg gagcgatttc taactaaaaa tcggaaggga ggtggtaagt tggagaaaaa
     2101 ggaagattta ttgaacgctt ttaagaacct agacgagaaa gatcaactta aagcgttatt
     2161 aaggcttcat gatataacat acagcgatat tgctgaaaga ttaaccgtta aaaaaggcga
     2221 gagggaagga caaaaaggtg ttagcctcgt gactgtacaa aaagctttcc aatcaaagac
     2281 caacttggag agtgatcgct ggaaagagat tgtaaaagtc gctaccaagc taattgaaga
     2341 gcgacaaaac gaccagaaca aagcataaaa aaagcccgtc catttaccgc tgaccaaagc
     2401 aataaaatga acgagccaca ccatttactt ttaggggtaa ccacacgccc taaaagctac
     2461 caaaccattt accaaataac cactaaggag atttagaaat gattagaaaa cttaataata
     2521 tcaagctttc tttggcaatt ttaacactat tttttggaat ttacaataat tccagaatgg
     2581 ctttgttttt tcttacgtta ttgatattca tgaacttaat tatccataat ttcaaggagt
     2641 tagaaaatac agctccagcg ccctctcaag gaaaccaaga aagtgagggc atgccacatg
     2701 cttggtagtt ttaaaaagga taaggaagta aaaacacgag gagcccttac accaagccag
     2761 caaaataagc ttggtacaaa agccactttc caccccgtcc gagatcaagc caaccaaaaa
     2821 gaactaattg gcgtttgtgt agggtttact tcagagaaat tttttccaat aatagagtgc
     2881 aaaatacacg ggatcacaac tagagaagtt gctagaaaag ttgtgaaatg gcacaccagc
     2941 caaaccaaaa ccaaccataa gaaacgttaa aaacgttcct aagagcgttt gaaaattaaa
     3001 ttaagggaga tttaagaatg aacaacaaaa acaccaaaaa taccaatact aaccacgaga
     3061 agagaagctt ctttgataag cttttcaaaa agaagaagaa tggaaaagta tctattttga
     3121 aaatgatcgg ctggatattt ttagcctttt tcatagttgc tgtgaacagt agcggcggtg
     3181 ataagaaaga ggctgcttca aaagatacca c
//
LOCUS       DACCWK010000158         3186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCWK010000158 DACCWK010000000
VERSION     DACCWK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.164"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..163
                     /locus_tag="IVW65_002573"
     CDS             <1..163
                     /locus_tag="IVW65_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7901417.1"
                     /translation="LARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILP
                     DGNEIKIGN"
     gene            179..1021
                     /locus_tag="IVW65_002574"
     CDS             179..1021
                     /locus_tag="IVW65_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901418.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1084..2886
                     /locus_tag="IVW65_002575"
     CDS             1084..2886
                     /locus_tag="IVW65_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP7901419.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(2945..>3186)
                     /locus_tag="IVW65_002576"
     CDS             complement(2945..>3186)
                     /locus_tag="IVW65_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7901420.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1142 a          442 c          645 g          957 t
ORIGIN      
        1 acttgctaga cgtattggac agactccaca gaatttcaat aagaaattac aacgagaaac
       61 ggtaacctta gatgagttga aagcgattgc tgatgtgcta ggtgtcaagt ttgtgcaggc
      121 ttttatattg cctgatggca atgaaataaa gataggtaat taggaggtgt aatttgtatt
      181 gaaaatcata acaataaatg atgttgagta tgctgtattt gctgctaatg aaggtacttc
      241 caaaccacaa cctcatatca tagaaacgaa atctggtact attccagaag gaaaacaact
      301 gagtttatta aaagaatatc ttaagcagaa tgatatttcc ccaataaaag gagcaacaac
      361 atattggtgc attgacaagg ttcttaagct tgattcaagt aaagaaaaaa ctataagtga
      421 aacaatccat aaacaaaaat acttatcttt aactgaagaa aatattgaga aacagcataa
      481 gtttgtaggt gcaagctcta attatgggaa agaaggtcta ataatacatg atgttttgaa
      541 tgcatttcct cttcataatg atttaaatac tattgcgatg aagattgcgg taattgatgt
      601 aacaaattca acacatcttt ctcaatataa gagcaggttg tctctatatg atttggctaa
      661 agtaattctg gagattccaa attttgatga tcggttggca aaaggagatc ctcagttgat
      721 aaatattatc gcaaggaaca ttggtgcagt taatatgttc tcatttgcat ccaagtactg
      781 tacatatcat aatgtggaag tgtgtgggag agatgattac tccatctttg atggtatcgt
      841 taaaaatact cttccacact atatccaagg actaactacg aataagattg atacgtggcg
      901 aagaagtttt gattacgagg catttaacga atgtgttgga aaactacttg atgaaaataa
      961 tatacatatt ccttttagaa gaagaaagtt agatcacttc ttgtggtatg cgaataggta
     1021 gaatataaga aaaaggcatc gtaaaagtga ttctggataa aattaatggg ggaggaaata
     1081 atagtgggat tatttgattt tctaaagaaa aaacaaggaa aggttagtaa acacgttgat
     1141 tatcaactgg cttctagtct atttcgtact ggtaaagttt ttgctgaaaa attggaagtg
     1201 gacatatcaa atgtagatga gtttcgtaaa acttttatcg cttttgatgt ggaaacaact
     1261 ggcctaaatt cttcttcaga tagaattgta gagattggtg cggtgctatt taaagaagga
     1321 gttccaattc aatcattcgg aactcttgtc aatccaaaag ttagaattgc agcctcagct
     1381 tcggcaataa ataatataac taatgaaatg ctaaactctg ctccaaatga gcaggaagtt
     1441 tacccccagt tggtagaatt tttaggagat gcactagatg gaaaaatcac tatgtgtgca
     1501 cacaatgcaa gtttcgattt tggattttta agtaacacat tttctagact tgggtatgat
     1561 gctaaaatta agtatgttga tactctgagt ctctctagaa aacatataaa aggcattgta
     1621 aatcataagc aagagactgt agcggaatat ttcggattaa taaatgacac tgcccataga
     1681 gcggaatcag atgcagatat atgcggaaag attttttggg gtgttttaga tattattgag
     1741 gagtcgttgg aagaacaaaa aaagcaaata gaaaagatga ctccagacat aaaagaactt
     1801 gaagcttgtg cttttattca agatattatt gagaaaaaag gatgcgacac cagttggatc
     1861 aggtatcgaa aaaacagtaa taattatgtc gatgtaactt gtctatatac attcttaaag
     1921 tttaagtttg ctaagaaggg aaattacatc attattagaa aagatgctac aaatggaatt
     1981 gatttaccta tagaaccttg tactgtaagt gaaggcggta caacaaatgt gcgagtctat
     2041 tttcgtagcc cctttgactt aacgcctctt tcaaattata ttttcagtgc ttatagcgat
     2101 tgttataatt ctatgaatga atatatgagt gttagtaatt acactaaaaa agaagcagag
     2161 caaagcctta gtatgctgaa atctatttca aaatcagcga tgaaagagtt attattcgat
     2221 gcaaaaaatc gtggatatga tgagactgaa gttaacctaa atattgaaaa agtgatatct
     2281 agagaagatg taattattaa cgggttgcat aatcgagttt cattaaacaa gatacgaaat
     2341 atagggaatt gggagaaagg atttgatgca ggttatccat attgggaacg tggagaaact
     2401 gctaggaaaa atggcaaagt agatgaagct attgcacttt ttgataaagc tcgatataac
     2461 ggctatgatg ctcctgcgct atatgattct tatgctaaag cttatcgaaa aattaaggat
     2521 tacgataatg aaattctcat tcttgatgaa ggaattatgc gtaaaacacg gcacgatgtt
     2581 ggtactttag aggctcgtag ggataaggca attaagctat tatttgctaa acaagaggca
     2641 gagcggatag ctaaggaaaa atctgatttt ctaaaagcga acaaaaaaga ggatgcgatt
     2701 agtgagccta gcaagaagcg tggaagacag attattcaaa tgactgatga tggaacaata
     2761 ataaaagaat tcgatactat agcttctgct gtaaaagaag ttggcgttaa ctcaaaaagt
     2821 attagggatg ctgcaaatgg agttcaaaaa catgctggag gatactactg gaaatacaaa
     2881 gattagcaga taatctaaga taacctagga tcttcttaat agatacccgt ggtgctagtt
     2941 aattttaata tcctaaagaa tatctcaaag ttccttcaaa acgtcgttgt gctcgactta
     3001 atagaacact gtaagccagt aatatgcttt caaatctact ctctagccct ttaacagaac
     3061 gtgagttgaa attttgacaa cctaattttt ctaaggaaga aaaaacagtc ccaactgttt
     3121 ttctttgttt tccaagtaag ctgtggtcta gtttttctga tttatccatg tttttacgtg
     3181 gcggaa
//
LOCUS       DACCWK010000159         3142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCWK010000159 DACCWK010000000
VERSION     DACCWK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.165"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(362..976)
                     /locus_tag="IVW65_002577"
     CDS             complement(362..976)
                     /locus_tag="IVW65_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7901421.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1582..1959)
                     /locus_tag="IVW65_002578"
     CDS             complement(1582..1959)
                     /locus_tag="IVW65_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901422.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2259..3140)
                     /gene="galU"
                     /locus_tag="IVW65_002579"
     CDS             complement(2259..3140)
                     /gene="galU"
                     /locus_tag="IVW65_002579"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7901423.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          955 a          651 c          486 g         1050 t
ORIGIN      
        1 atgataacaa atgtactata attactttgt aaagagtttt ggggaagact ctttcatcaa
       61 cgtgtattaa gagacaagaa agaatccata tggattcttt cttgtctctt tttccactac
      121 tttttatttt tctgacacta catattctta taactcattt ctcataactg tgaaacaact
      181 tataaaaata acaaacttta aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa
      241 taactaaaac aaaaaaagta gtcgttgtta atcctcatta ataatcgttt tctttttttc
      301 aaacgctcca tttttatgtg gaacattatt tttttaagga actttgtcaa catgtggata
      361 actattttcc acttgtgtcc tggactagct gttttgctgt aaaatacaaa acttgctgca
      421 gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc tggatctaga tttggcaaag
      481 tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca aattcaaata
      541 atcgcaaacc actgctatgt tgcgatttga tacttcgtga caaatcaaat ccagtaacaa
      601 tgattaatgg atgaccattt tctttgatcc actgagctag ttcgatgatt gccgggtttc
      661 ttccttcatt cgaaagaaga agaaaaatgt cttctgattt gacagaaagc tgatcaataa
      721 ttgcctgtcc tgcaccttct agcgtttcat agacacctcc ggctggattt tcaatcaata
      781 tacttggtac aagatccggg gtttgatccc aaaattcaaa tgctacacca cttagtgtgc
      841 ggctagcata tatctgcaat ctaccacctt gattaatttg tttcgcgatc caagtgccag
      901 cttgaagcat cagcggctct tctatcctta atttctcttc caattggtga taatagtcaa
      961 aataacataa actcatttat ctctcccgcc tagctatact tgacatgaat agaaacagct
     1021 gcctaatcta aggaagtaca taaaaaaaca gtagttagac agtaacacat gtggtctttg
     1081 ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca ataccttgac atagcaaata
     1141 aaacaacgtg cagaccatgg aaaatatcta cataggggta gaattttcac cctagattag
     1201 gtacaataaa ttaggtcgat gcactgcatt gactatggca aaatgatcgg tcagggtgta
     1261 tgtgtgtgac tggtcattcc ctgattctta gcgccaacta agggtcaggg agcctttttt
     1321 tattcagctg tttctatctg aatttattgt aagtaatctc ttatccaagt gcaagcaaga
     1381 aaatacaaaa aaacattggt atgatttata tatatataaa taacataaaa aaaggaatgg
     1441 cttatcccga tacaacaagc attcctttaa aaaagattct tttatataag tgaaaaaagc
     1501 ttttttccac caattaaata aatctctcat ctcaatagtt tatccattat tttatctttt
     1561 tttgcgtata cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga
     1621 atctgtacta gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt
     1681 catatatggg atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc
     1741 ttcccaacta cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata
     1801 ccagataaaa atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact
     1861 aataagcaat agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat
     1921 atcaagcagc gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt
     1981 ataattaaaa aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct
     2041 cttatgtaat cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt
     2101 ttctgaatct attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct
     2161 agagtcaaaa aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac
     2221 aaatagcctt tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt
     2281 ttactcaact ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca
     2341 tattcaatat ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta
     2401 aactctctcg catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt
     2461 atctcgtttc ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt
     2521 agataacgtc cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca
     2581 aaccctttta ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat
     2641 ttagatgttt cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca
     2701 taatccatca tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc
     2761 atgacgacaa acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt
     2821 cctcttggat gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa
     2881 gcgagtaatt ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca
     2941 aagtgatctt caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc
     3001 gaatccaatg cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt
     3061 tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg
     3121 actgcttttt tgaccttcac tt
//
LOCUS       DACCWK010000160         3096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCWK010000160 DACCWK010000000
VERSION     DACCWK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.166"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..186
                     /locus_tag="IVW65_002580"
     CDS             <1..186
                     /locus_tag="IVW65_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901424.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="IVW65_002581"
     CDS             329..553
                     /locus_tag="IVW65_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7901425.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="IVW65_002582"
     CDS             568..1437
                     /locus_tag="IVW65_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7901426.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(1641..2960)
                     /locus_tag="IVW65_002583"
     CDS             complement(1641..2960)
                     /locus_tag="IVW65_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP7901427.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          997 a          514 c          625 g          960 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgacct
     1441 gaataattca tagctttaat tctttggcac attttattga aatatgtatc actaatatcc
     1501 ctatcaactg attttgacta aaaagttact tcaaacattc actgttcaaa aatattgagg
     1561 aataaatttt gggtgtaagg cattttgagc atacttctcc ctggataact tcccagtaaa
     1621 aaaatcgtta gattgttgga ttagatccat tgactggctc gcctcaggcg agcaaagacc
     1681 ctgtagaatt cattctgata cacatgataa ctagataatt tgagatagac tcgtctaccc
     1741 gtttggacta atttcccagc aactttcaga aacttcaatc gaatagaatg aatggtcatt
     1801 cccttttgga cttcttcaaa gccaatcgtt cttaagaagt tgactaagtt gtaagctatc
     1861 aagctgagca tcattcggac atgattctcc aaaaagcgag gactgtctgt tttgtcaaag
     1921 taaaagccag ctttcgcttc tttaatgaag ttctccattg tgccacgttt ggcatagata
     1981 gaaaagatgg tatcaggaga aacattttct gatagattcg tcacgataaa ttcatgtcga
     2041 aagagtagtt cgcccgcttc acgtgttgag cgtatacaca cgcgacgact ttgtgaccag
     2101 gattgtgctt gataagtggt cgagaagtac tgaacttctc gttcttccca cttttgatta
     2161 tcgccataaa gtactgattt ctcagctatt tgacctagtc tacgattatt cttcagtcga
     2221 ataacataat gactcttttt tgattcacac gaatcataca caccaggtgt agcgaacccg
     2281 ctgtctccac gaaccaagat gtcagtgttt ggtagagagt gattatagtg ctctaataaa
     2341 ggtgtgagaa actcctttac gccttttgat gtatattgat ttcctgaacg tagttcagct
     2401 tttaagaagt caccagtcaa tccgtcaaaa gctaccaatg gatggtaacc ataggtttgg
     2461 tagtgtgtat tataattcgt ttgttcttgg tgaccaaatg tatctgaatg ggtcgaatct
     2521 aaatcaatga ttaattccgt atcattacgg atgaggcgtg ctttatcgat aagttcttgg
     2581 ttcaaagctt gaagttcatg gatattctcc tcagatagtc gatctaaaaa tcgggaaagt
     2641 gaagattgag aagcgagttc tttcctatct aaaacagctt taaacacagg atcttgtctc
     2701 aggagattag ctgcagaatc agctgagtaa ccagcaatta attgcataat gaactgctca
     2761 agtatggata agttgtcatg agtaaaatac gctcgttcgt cttcaatgtg aatgtgttgc
     2821 ttagccagtt cagaaaaacc aaagatgttc atcagctctt tcactaggac gagacccgaa
     2881 tcactcgata attgaccacc tgtatgagaa atagtgatat ttgaattgaa ttttacttgg
     2941 tttttgtgta agctagtcat gaaaagaact cctttctttt ggttggttta gtcactttaa
     3001 ccatagcaga aaggggttct tttttcatca cttaacgggt gaagatgaaa aagtaattgt
     3061 aagctgccgt gaggcagttt cacatggttt taatta
//
LOCUS       DACCWK010000161         3040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCWK010000161 DACCWK010000000
VERSION     DACCWK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.167"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(248..469)
                     /locus_tag="IVW65_002584"
     CDS             complement(248..469)
                     /locus_tag="IVW65_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901428.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="IVW65_002585"
     CDS             complement(567..812)
                     /locus_tag="IVW65_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901429.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="IVW65_002586"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="IVW65_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="IVW65_002587"
     CDS             complement(2036..3037)
                     /locus_tag="IVW65_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP7901430.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
BASE COUNT         1003 a          534 c          404 g         1099 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta
//
LOCUS       DACCWK010000162         3021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCWK010000162 DACCWK010000000
VERSION     DACCWK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.168"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(287..649)
                     /locus_tag="IVW65_002588"
     CDS             complement(287..649)
                     /locus_tag="IVW65_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901431.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IVW65_002589"
     CDS             complement(723..1103)
                     /locus_tag="IVW65_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7901432.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IVW65_002590"
     CDS             1185..2360
                     /locus_tag="IVW65_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7901433.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2436..3020)
                     /locus_tag="IVW65_002591"
     CDS             complement(2436..3020)
                     /locus_tag="IVW65_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7901434.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          577 c          498 g         1004 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt
     2401 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2461 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2521 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2581 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2641 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2701 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2761 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2821 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2881 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2941 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     3001 tctagttgtg ggaatgccat a
//
LOCUS       DACCWK010000163         2977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCWK010000163 DACCWK010000000
VERSION     DACCWK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.169"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(37..1257)
                     /locus_tag="IVW65_002592"
     CDS             complement(37..1257)
                     /locus_tag="IVW65_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7901435.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IVW65_002593"
     CDS             complement(1277..1882)
                     /locus_tag="IVW65_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7901436.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IVW65_002594"
     CDS             complement(1931..2863)
                     /locus_tag="IVW65_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7901437.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          814 a          623 c          516 g         1024 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttat
//
LOCUS       DACCWK010000164         2961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCWK010000164 DACCWK010000000
VERSION     DACCWK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.170"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1458
                     /locus_tag="IVW65_002595"
     CDS             <1..1458
                     /locus_tag="IVW65_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7901438.1"
                     /translation="VPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPE
                     GTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVE
                     GEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTK
                     DTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKV
                     DVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDAS
                     EGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPN
                     KPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVI
                     VTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFP
                     KTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1592..2935
                     /locus_tag="IVW65_002596"
     CDS             1592..2935
                     /locus_tag="IVW65_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7901439.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1164 a          407 c          579 g          811 t
ORIGIN      
        1 gttccagtag aagtgacaga caatcgttcc gatgccgata aatatgaacc aacagtagaa
       61 ggcgaaaaag tagaagttgg gggcacagta gatctaacag ataatgttac taacttgcca
      121 gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac
      181 acaccaggta attacgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg
      241 gtgaaagttc cagtagaagt gacagacaat cgttctgacg ccgataaata tgaaccaaca
      301 gtagaaggcg aaaaagtaga aattggaggc acagtagatt taacagataa tgttactaac
      361 ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat
      421 accaacacac caggcaatta tgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa
      481 gatacggtga aagttccagt agaagtgaca gacaatcgtt ctaatgcaga taaatacaat
      541 cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc tgagggaatt
      601 aagaacaaag aagatttacc aaaaggaact acttatgctt ggaaagagaa agtagatgtt
      661 agcacagctg gaaataaaaa aggtactatt gtagtgacat atccagatgg atcatctgat
      721 gaagttgaag tagacgttac agtgacagat actcgttccg atgcagataa atataatcct
      781 gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga gggaattaag
      841 aacaaagaag atctaccaaa aggaactact tatacttgga aagaaaaagt agatgttagt
      901 acagctggaa acaaaaaagg tactgtagta gtaacatatc cagatggatc taaagaagaa
      961 gtagaagtta ctatttctgt agtagataaa aaagcaccga ataaacctca agttgatcct
     1021 attacagagg gtgaccaaat tgttactggt aaaactgaac caaatgcaga ggtgacagtt
     1081 acattaccag acggaagtca acaccatggg acagctgata aaaatggtaa ttttacagtt
     1141 aaagttccta aattagaggc agggacaaaa gtgatagtaa ctgcaactga tgaatctggt
     1201 aatactagtg aacctactaa tgtagttgtt tcttccaaca aaaaagacag tggaaaaaac
     1261 ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga caaaaataga
     1321 ggcaaaagcc agtcctcaaa agtttttcct aagacgggag agagtgacag taatatcttt
     1381 actattagcg gtggtttaat actgttagga acgttaggat tattagggta taaaaatcga
     1441 aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc tctgagaaga ggaagaatct
     1501 ttttcttcct ctttttgctt ttttatgatg ggaaataagt gttagttaaa gatgaatata
     1561 ctttttatag aaaggaaaag aatgtaatta agtggaaaaa atagtaatta ttggttcaaa
     1621 ccattcaggg ctagccgcag ctcgatttct aaacttaagc gattcaaatt atgaagttat
     1681 tctaatcgat aaaaaatcag aaattagtta cttggaatgt gggacatcgt tatttataaa
     1741 aggagaagta agtaatttag aagatctttt ttatcaaaat agaatgacac taaattctga
     1801 gataaaaaag ttatttctag agacagaagt tgacaaaatt gatattcaaa ataaaaagat
     1861 tagttgtgta aaaaaagatg gtacaagatt ctctcaagaa tatgacaaag ttatattagc
     1921 tacaggttca aagcaaatta tgctagatat accggggaat aatttagcta atattttttc
     1981 gataaagaat tttccaaatg caaaagagat ttttaaagct ttggatgata gtactattaa
     2041 aaaagtagga attattggag caggatatat tggagttgaa attgctgaag ccataagaaa
     2101 aagagggaaa gaagtatatc ttttcgatgt tgcagataga gtgttgtcga cttattacga
     2161 ccgtagtttt tcagataaag ttgaagaaat actatcaaaa aatggaatac atctttgtct
     2221 atcagaaagt cctaaaaaat atttaggaag aaaaaagatt gaaaaaatag tcactacaag
     2281 tggtacccaa tactctatgg atatggtcgt acaagctata ggatttctat ctaatagtcc
     2341 aattggggaa aaggaactct tgagagatgt tagtggagca tatctaacta atgaatacca
     2401 gcaaacaaat ataaaagata tatatgctat tggggattgt gctacaactt tttttacgtc
     2461 cattaataga agtagtgttg atttttctat ttctaatgct ctaagaacag cttatattgc
     2521 aactcaacat attaacaatc aagattttac accttctggt tcccaattta caaatggctt
     2581 ttctatattt aattacaatt tttacgcagc aggtttgaat ttaaaaacag cacgattatt
     2641 aagtgagtct atagattata tagaaataga agatttcatt agacctgctt ttttgagaaa
     2701 aaatagtcgt gtaaaattaa gaattagctt tgaaaaaaat acaaagagaa tagtaggtgt
     2761 acaactttgt agtcaagaag atttatctgg actattatca atgttttctc ttgctataga
     2821 agaacaaatt acattagaca aattgaaaat gttagattat ttatttcatc ctagttttag
     2881 ccaaccctat aattttattt taaaggcagt aacaggatta aacattgaaa cataatcgtt
     2941 actaaatgaa atcaagaaat a
//
LOCUS       DACCWK010000165         2940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCWK010000165 DACCWK010000000
VERSION     DACCWK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.171"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..820)
                     /locus_tag="IVW65_002597"
     CDS             complement(<1..820)
                     /locus_tag="IVW65_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7901440.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLK"
     gene            966..1769
                     /locus_tag="IVW65_002598"
     CDS             966..1769
                     /locus_tag="IVW65_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7901441.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1947..2894
                     /locus_tag="IVW65_002599"
     CDS             1947..2894
                     /locus_tag="IVW65_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7901442.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT          959 a          565 c          471 g          945 t
ORIGIN      
        1 attttaataa agaaattata cgaggatata tcttttcaat ctcgcttagt agctgatctt
       61 caaaatcaat atgtccgtcg aaaataatta tcttttcgct atactgccaa atatggacat
      121 gatgaacatt ctttacccca tcgattgctt caatttcttg ttccattttt tcatagtcaa
      181 tagcaggtgc actttgtatc aaaatagcgg cagctttttt gatcaccatt atcgcttcta
      241 tcaatatata tatcgaaatg atcagtgtaa taactggatc gatccagtag atatcgaaaa
      301 aacggatcaa aattgctccg ataatcacac ttaccgaaga cagtgtatca cttaacaaat
      361 gcaaataact agattttata ttcaaacttt catctgcttc ttgtcgtaat aataatactg
      421 agaacaaatt cgcgagcaat ccaataactg ctactgttag catcaaatga ctatttatct
      481 tttctggtga agtgaaccgc ttgaatgctt caacggttaa aaacaaggat aatatgatca
      541 atatgaatga gttgatgaaa gcactcaata tttctaaacg ctggtagcca tacgtacgtt
      601 ttttatttgc ttcttttcgg gccatttttt gtgccccgta agaaaaaata attgccaatg
      661 tatcactaag gttatggaaa gcatctgaaa caagagacaa actccctgac ataatccctc
      721 caataaattc tgataaagtg atcaccgcat tcaaaaccac tactccaaat agtttttttg
      781 atgatctctg tggctgtgta ggcactattc cttcctccat tttatttata ttggatagtc
      841 tttagacaag aataacattt ttttgatatt tcaccaatca ttcagatgtt tccctaaagc
      901 acttgtattt cacttgatac ttcgctagaa ttgcaagtga tgacactact agatgaggag
      961 tacttatgac aacaaaacaa ttaattatca gttcctttat acgattcaaa gacctaaagg
     1021 agacgagaaa tgtcccattt tggaaagcca ttgtctatct tttagttttg agtataatta
     1081 tggctttgcc aatctctttt caagtttttc aagtcttaaa caacattcaa ttggatgggc
     1141 aaaagattgc tactcgcatt cctgatttta caatccaaaa cgggcagctt caaaccgaag
     1201 aaaaagaagg attcatttac caaacaaact ccattatctt cacatttgat ccagagggaa
     1261 aacgaacaga acaggacatc tctactgatt tgatggggaa ttttgttagt gtcgggatgt
     1321 taaaaaacaa acttatcata gctcttccta atacaggcac gacttctgct ttgttgaaca
     1381 ataaccaatt agaacttccc tacaccaatg aatctttaaa aaacttaaca ggtaaggagc
     1441 tccgttcttt tttaagtaaa gcatctatac cactttggat aaaagtcctg acatttatat
     1501 tttctattta tcctagtttt ctgaatctgc tgatcacttt gctatttacc aacattgctg
     1561 cttttctcta tgcacgcttt cgactaacaa aagctacttt tcttgattgt ttaaaaacat
     1621 tgatctattc aatttcattg cctactataa ttgcaacaat cttgatggtc tttcttcctt
     1681 cttttgacac aagcgccttc attgcttttg caggaatctt tatttttgct caagctgtca
     1741 aaggatgggc aaaaatatcg atcaattaag aaataaagac aactcgttat ttctacttca
     1801 atagaccctt ttagaggttg tgacattttt ttgtcacagc ccctttttta tttgaaaaat
     1861 atttctttct caaaaaacaa gcaaggcgct tcaaagttct tcttgtatgc gctacactta
     1921 gagtaaagaa aggcgggaaa ttaactatgt cagaaagaat catttatgca gatcgtccat
     1981 ttcgtgatga atttatcgaa gaaatccata ctatagcgcc tggttatatc tttaaaacag
     2041 aattacaacc agaggatcta caaaatgtag agatcagttt gggctggaac tcagactacc
     2101 aagaaaaact attagcctct tctaccttga aatgggtcca atctatctca gctggtgttg
     2161 atcagctacc ccttgagact ttttcaaaga aacatatttt actttcaaat ggtagtggca
     2221 tccacagtga atcgattacg gagcatgtct taggaatcat ccttgggtat tctcgtggtc
     2281 tttttcaagc acaaaaagca cagagtgaca tgaaatggtt aggttcatcg gtccattacc
     2341 aatctatcca aggaaaaaaa atactaatca ttggaactgg ccatattgga aaaatgattg
     2401 caaaaaaatg caaagcatta gacatgaact gctatggaat caatacatcc ggtcatccag
     2461 cagaaggaat ggaaaagact ttttctttaa atcaactaaa ggaagttctt cctgaagtag
     2521 atttcgtagt caatatctta cctctaacgg aggaaacaac tgggctcttt gatcagcata
     2581 tgtttgaaca ctttgatcca aaatctgtat tcatcaatgt tggccgtggt gcttctgtta
     2641 agacacaaga tctggtgcag gctttaaata accagcaact ttcctttgca gcattagacg
     2701 tttttgagga agagcctctt ccacaagatc atcctctttg gaaaatggac aatgtactga
     2761 ttacttccca cattgcaggt ttgacacctg attttcaaaa gaaattaatg gctatttttt
     2821 taaccaatct gaaaagctac tttgccaccc atgagcttca aaccaatcaa gtaaaattgt
     2881 ctgcaggata ttaatagctg aaaacaatat tttcaaaaga acaaagagaa ggtgacaaaa
//
LOCUS       DACCWK010000166         2917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCWK010000166 DACCWK010000000
VERSION     DACCWK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.172"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            133..948
                     /locus_tag="IVW65_002600"
     CDS             133..948
                     /locus_tag="IVW65_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901443.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1074..1331
                     /locus_tag="IVW65_002601"
     CDS             1074..1331
                     /locus_tag="IVW65_002601"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901444.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1583..2155)
                     /locus_tag="IVW65_002602"
     CDS             complement(1583..2155)
                     /locus_tag="IVW65_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901445.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2171..2776)
                     /locus_tag="IVW65_002603"
     CDS             complement(2171..2776)
                     /locus_tag="IVW65_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7901446.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          955 a          529 c          454 g          979 t
ORIGIN      
        1 gactagagaa ttaaataaaa aataattact atctagtacg aaattaatta cataggataa
       61 tatattaagg tgctggacta caagtaaaaa atactgtatg atcatgacag attcaagaaa
      121 ggagcaataa gattggttaa gatagcagga ctttctaaag gtggaaaaac cgtttcacaa
      181 ctttataaac aaaactacag agactggaga cagttcagaa aaaacattaa ggattcttac
      241 tttattctac caacagaact tgaaaaatat ttaccgaata acaaagctat taatttatat
      301 ctgtattact gttttaaggc aaagaatgaa ggcggggatt cctggcattc ggtcaatacc
      361 atagcagagt ctctaggagt gacaactaaa actataaaca cctggaataa aaccttagtt
      421 gatttaggta tgattgcacg tatagacgat aaccatctca gtaaatcgac aattttactt
      481 cctctctcta gttactataa attagccctt gatagttctt taaaggatgt taccgagagc
      541 actgtccatg aaattgatgg caacttggtt gcagtttatc atttgtttga atggagaaaa
      601 aatgaggctg atgaaaatta taacgttcct tataacacac tctgttgtgt ttttaaaaga
      661 aaaacacaaa aagatactat ctataaattt atcttggtcc aagatgagaa aataagtaat
      721 ttcgaattaa gcactccttc tagtaagatt tatgacgatg catatagatt tgaatgtcaa
      781 gatctagaag ccctcctttc cgcaaataat ctaaacaata tagaacatga aaactttgtt
      841 gtaaacacta gatttaattt agtttctgaa aaggactcag atttgttaga tttgctgata
      901 gagcttacta aaaatctaga taagaaagaa aacattactg tactataact agcatcggtc
      961 catcattaag tttggactat tagaacactt gctattacag aaaaaggtat gctagccacg
     1021 cttactattt attggtatca aacaagtatc ttaatttgaa aggaagatga tttatggcta
     1081 atttatatat agagaacaaa ggagacttga atgccattat tactttaatc gtagttatgg
     1141 taaaatcaat aggtaatatc acaataggaa tgctgtttat tatattagca gcagtgctga
     1201 tttttatgat agttatttgg ttcttaaatg aaagatctaa agacaatcaa caccgtaggg
     1261 atatggagat aaaatcaata gagtcaatag attatttatc gataataaca ctaaaaaagc
     1321 cacgttccta aacgtgactt acgctgaacg atatgtgttg tttggtagac gaacaaatcg
     1381 ttcattataa atatatgatg gtatcctaat atagtaagcg tggctagcat acctttaatt
     1441 actttacctt ttacagttac catacacatt ttttgagcag aaatcaagta aataaaaaaa
     1501 tattatattt cacaacatgc taatagactc atatctagaa cgcattaagt atttacctgt
     1561 ctggttcgct gagacttctt ttttaacttt cttcaatttt cttctttatt ctaaaaagcg
     1621 tactgcgact gaaaccggtt atagcttcaa cttccttata cgatttacct gaagtaagca
     1681 gttcatatgc gtgcctcttc ttcggtgtga ttgttgcttt cggacgtcct tctctgaaat
     1741 taggattatt ctttcttgca aacatttttc cttcttgggt acgttccaca atcatatcac
     1801 gttccatttc tgctacactc aacaacgtcc gtgtcaccat tcgccccatg gacgtatttt
     1861 caatcgttcc tagattcaaa accttgatcg ttacgttatc atcgagtaaa ggttcaatga
     1921 tctctaatgc ttctctcgtg ttacgagcaa atcgatcaag cttcgtaaca actatttcat
     1981 ctccaggctt cagaccagcc aacagctgat tgaattttgg ccgatcccgt tttgtacccg
     2041 taaatttttc tgaaaaaata ttcttcttct ctacgccgtt ctcaagcaac tgttgaattt
     2101 gttcttttgt actttgagaa gcggttgaga cacgtgcata tccatatttc gtcatttggt
     2161 ccacctactt ttacaaatca tctatcgtat actcagaata cccttcatag aaatagctga
     2221 catcgattcc tttcatatat aactcgcgat cgtcaatttt atcagtcaga gcattggaaa
     2281 ttagatagcg tatttccaaa tcgtttatgg gacttcgctc cattgcagaa agatagtctg
     2341 ctttatcgat taagttccaa tctacaactt tttgcaattc ttcctttaaa atgagatcca
     2401 gccaaattct agtacttcgg ccatttcctt ctcgaaatgg gtgagcgata ttcatctcca
     2461 catatttctt gataatctct tcaaaagaag tttgtggcat ctgatcaata tgctgtaacg
     2521 aatgttctaa atacataact ggtgcaaatc gaaaatttcc ttttgcaata ttgaccgatc
     2581 ggatttttcc ggcaaaatcg taaatatccg aaaatagaaa ttcatgaatt tcagataaac
     2641 ctttaaatgt accaacttcg atttcattta tttttccaca atcgtacaat tctttcgctt
     2701 tttgtttact gagtttttct tcaaccttag ccaactcaac ttgattggtt aatcccaatt
     2761 tattctctaa aatcatgcga aatctcctta actttatgac ataagtttat gaaacactct
     2821 caaccccatt atatcgcgat tttgtttatg tgtcaaaaac tattaagttt ctgacccccg
     2881 atagattttt caagtgtctc tattttgcac agtctag
//
LOCUS       DACCWK010000167         2916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCWK010000167 DACCWK010000000
VERSION     DACCWK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.173"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..539)
                     /locus_tag="IVW65_002604"
     CDS             complement(<1..539)
                     /locus_tag="IVW65_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7901447.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQG"
     gene            674..1063
                     /locus_tag="IVW65_002605"
     CDS             674..1063
                     /locus_tag="IVW65_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901448.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1067..1831
                     /locus_tag="IVW65_002606"
     CDS             1067..1831
                     /locus_tag="IVW65_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7901449.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1960..2907
                     /locus_tag="IVW65_002607"
     CDS             1960..2907
                     /locus_tag="IVW65_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7901450.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
BASE COUNT          929 a          497 c          606 g          884 t
ORIGIN      
        1 tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata ttttggctca
       61 taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag ttcacgttcg
      121 ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc cattgcagaa
      181 gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt atcttctgcg
      241 ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt gtcttcaatc
      301 aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa attcagtgga
      361 ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat ctcgaccata
      421 ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg taacgtgttc
      481 agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg atcaatcatt
      541 tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa
      601 ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta taaagatagg
      661 ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg tgctaagtct
      721 ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg tagagaacat
      781 tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct ttttgatact
      841 gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta tttatccact
      901 cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc ccgagccatt
      961 cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg ttggagcatg
     1021 gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga atccaattat
     1081 attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag aattaagaga
     1141 ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg agttagtttt
     1201 gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag atgcgatgaa
     1261 tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt gggatagtat
     1321 ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa gaggtcttct
     1381 acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat gcgccttgtt
     1441 ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg cacctttgga
     1501 atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac caaaagacta
     1561 ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg aaaagttagc
     1621 tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt atatacaaac
     1681 ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg tttttcgttc
     1741 ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt ccactttttt
     1801 agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc atgacgatag
     1861 aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat ttggtataat
     1921 tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt agcagatgtt
     1981 gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca tccggaacag
     2041 gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga ttaccgtcca
     2101 aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt atttatactg
     2161 gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat cgctaaacgg
     2221 atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga cgtcggttct
     2281 tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat cgaccgtttg
     2341 gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt agacagcgat
     2401 aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta tcaaacgatt
     2461 atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac tggttattta
     2521 tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa tcattcgaca
     2581 gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac ttgttttgta
     2641 tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc tggaggacac
     2701 ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca aatcagttca
     2761 cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg tggagaaatg
     2821 ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt ttttcctgaa
     2881 ctagttatca gagagacaac gaattaagtc ttatta
//
LOCUS       DACCWK010000168         2793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCWK010000168 DACCWK010000000
VERSION     DACCWK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.174"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            239..1087
                     /locus_tag="IVW65_002608"
     CDS             239..1087
                     /locus_tag="IVW65_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7901451.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1232..2172
                     /locus_tag="IVW65_002609"
                     /pseudo
     CDS             1232..2172
                     /locus_tag="IVW65_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
     gene            2162..>2793
                     /locus_tag="IVW65_002610"
     CDS             2162..>2793
                     /locus_tag="IVW65_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901452.1"
                     /translation="MTIKREKWLRGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSILWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYWYAPFLSLMAYFMMLTVFRKKDLLYEMIAV
                     VTVLFVYLAARQFFSIRLLSGNTRFQSLTTVLLICFLTFSAILPLYLPKV"
BASE COUNT          821 a          531 c          521 g          920 t
ORIGIN      
        1 tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt aaaaaaacat
       61 aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat aaatagcgat
      121 tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac aaaaaatcga
      181 tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta tccaattgat
      241 gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg gtgtaggtaa
      301 aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca gagtccaatg
      361 tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt cacaatcaaa
      421 agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttggcag atgagatcaa
      481 tcgtacgact cctcgtactc agtcagcact tttagaagca atggctgaga atcatgcaac
      541 gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta cgcaaaatcc
      601 aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt ttttattccg
      661 actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg ataaattcga
      721 aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag aaataaaaac
      781 aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc agttagtgtc
      841 agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa gcctttcctt
      901 tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca cgcccaaaga
      961 tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg atcgttcatt
     1021 gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc ctattcctgt
     1081 gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat tttgtgttat
     1141 ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct ttaggctggt
     1201 tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta agcagctttc
     1261 taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa aaaagtatgc
     1321 aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct cgtttacaaa
     1381 tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta caaataaacc
     1441 gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa cattaactgt
     1501 catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa gagagatctc
     1561 ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat tacagagagt
     1621 tctggaaaaa aacagcagct gaacgcgtac caaagaggtc ttgattttcg ggaacatcgc
     1681 gcttatcgtc caggtgattc tttcaaccga atcgattgga aattgtctgc tcatactcaa
     1741 gagtggttat accgtgaata cgattatcaa gaagaagaat gttctcctct tttttgcttt
     1801 tggggttcgc tcagtccaaa atttgaacct ctgctagact tgtatttttc tttatggcat
     1861 ttgagaaaag aaaagcagcc ggaacttctg atattaggca accgcgcctt tcatggcaaa
     1921 gaccccggtc atacctattt tgcttctttg gaagctggcg atgagaaagc ttttttcgca
     1981 cacttgaaaa aatatgcaaa aaaacaaatc gtcctctttg ttcctgagaa ttctcctgaa
     2041 gtctcagaag ctattgaaac attgtcaaaa gatcgaacgg tttatcttgt ttatttcgta
     2101 gaaaaacaac tcatggtcca agaaaaagac aagatatgtt cattgccagg aggtgaatgg
     2161 attgacgatt aaacgggaaa aatggctgcg tggcgtttac tcctttgtga tactaagtgt
     2221 aggtgctccg atatttgtag tagctaatca attgcaaaac agctaccttc ttatcctaac
     2281 tattggtgtc ctatgcctta ttgtctgctg tattcctaaa aaatgggtac gtttgctgct
     2341 ttctttgcca gtaatgatag gttgtttgtt tttatatttt ccttcgaaga cattttccat
     2401 attatggatc ttacagtttt tttcgtcagt cacagaggaa ttcttccatc tatcccaagg
     2461 taaactttat tatttgccag aaaagacagc tttttttctc ataatgttgc tgatctattt
     2521 atttattaca ttgaccgtgg attacgacta ttggtacgca ccctttcttt ctttgatggc
     2581 ttacttcatg atgcttaccg tttttcggaa aaaagattta ctgtacgaaa tgattgcggt
     2641 agtaactgtt ttgttcgtgt atctggcagc aaggcaattt ttctccatcc ggttgttgtc
     2701 gggaaacact cgattccagt cattgactac agttctgctt atttgtttct taacttttag
     2761 cgctatcttg cctttatatc ttccgaaagt aca
//
LOCUS       DACCWK010000169         2651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCWK010000169 DACCWK010000000
VERSION     DACCWK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.175"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            187..1212
                     /locus_tag="IVW65_002611"
     CDS             187..1212
                     /locus_tag="IVW65_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7901453.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            1387..2631
                     /locus_tag="IVW65_002612"
     CDS             1387..2631
                     /locus_tag="IVW65_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7901454.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
BASE COUNT          909 a          479 c          580 g          683 t
ORIGIN      
        1 gatgaaaaat tttttatttg tttttttatt ataaaaaaac acttatttga caaggatgta
       61 aacgactttt tggtttatca acaagtttat ccactattga caaatagatt atccacaaac
      121 agtggaaaac ttgaggataa actcagacaa aataagaatg tatgaaaaag gggttgtgga
      181 taaactgtgg aaacgaaacg atatgatgaa acagaattgc aagaggcagc cgaagcactg
      241 aaagaaggag aacttgttgc ttttcctacc gaaacagtat acggattggg cgccaacgct
      301 cttttaccaa acgctgtcaa gaaagtgttt tctgtaaaag ggagaccgca agataatcca
      361 ttaatcgtcc atgtcgcctc ttttgaacaa gtaaaagagt atgtggacaa ctttcatccg
      421 gcagcagaga agattgtgaa gaacttctgg cctggaccat taactttgat atttaagatc
      481 aaaaaagaca ctttgccctc agttgtgacg ggaggattat ccacagctgc atttcgtata
      541 ccggataaca aaaaaacact agaagtaatc gaactttccg gtgttccgct tgtcggtcca
      601 agtgcaaata cgtcgggaaa accaagtcca acgactgcag atcatgtata tcatgatcta
      661 caaggaaaaa ttaccgggat tattgatgat ggagctacgc gaattggtgt agaatccact
      721 gttcttgatt taagtgatcc aacagccatg cctatgattt tacgaccagg tgcagtaacg
      781 aaagaacaaa tcgaagcagt catcgaatct cctgttgcta ttgatcagca tcttgtaaaa
      841 gaaaatgaaa caccaaaagc ccctgggatg aaatacaaac actactcacc cgatacacgt
      901 gttctgatgg tcagagatgg cgattggtca actgcagttc aatgggcaaa aaataagaag
      961 atccgtgcag gtgtcatagc aagccccgaa attgcggatc aagtgcggac agatactgca
     1021 gctgtttata tgtataacga caactcagta gaagcggcgg ctaaaggatt gtttgcaggt
     1081 ctaagaggat tagatgaacc aacacttgga ttagatttga tttttgtaca agtgtatcca
     1141 gaaactggat tggggaatgc ttacatgaat cgcttgaaaa aagcagcagg acaaaattat
     1201 tttgaaaaat gatcaattgg ttattgtcgc attcttttag tatgatacaa tgctatcaag
     1261 taaatcttct atcagtgaac gaaaaactaa gcgtaactaa tcgtaataga agagatacga
     1321 atatttggca atttgttttt gatgggaaaa ataaaattgt aggaaaataa aaaggagctg
     1381 ttgtttgtgg tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa
     1441 gaagaaagac aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca
     1501 gtgatggctg cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat
     1561 cgctattatg gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca
     1621 aaagaattat tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac
     1681 acggctgctt atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc
     1741 gcaggcggac atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt
     1801 gttgcttacg gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta
     1861 gctagaaaac atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc
     1921 gactttgcga aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg
     1981 gcacatatcg caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat
     2041 atcacaacga ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca
     2101 aacgatgaag cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc
     2161 cctctagagc atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca
     2221 tttaaagaat atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat
     2281 caagcaattg gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat
     2341 gttcgcgagt taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc
     2401 actgtaaata aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt
     2461 cgaatcggca cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg
     2521 gctgaactag ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc
     2581 aaaacaggtg tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa
     2641 ttccattagt a
//
LOCUS       DACCWK010000170         2650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCWK010000170 DACCWK010000000
VERSION     DACCWK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.176"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            36..197
                     /locus_tag="IVW65_002613"
     CDS             36..197
                     /locus_tag="IVW65_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901455.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IVW65_002614"
     CDS             210..713
                     /locus_tag="IVW65_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7901456.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IVW65_002615"
     CDS             773..1162
                     /locus_tag="IVW65_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7901457.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IVW65_002616"
     CDS             1149..>2650
                     /locus_tag="IVW65_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP7901458.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          789 a          439 c          609 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 attcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACCWK010000171         2498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCWK010000171 DACCWK010000000
VERSION     DACCWK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.177"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            30..224
                     /locus_tag="IVW65_002617"
     CDS             30..224
                     /locus_tag="IVW65_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901459.1"
                     /translation="MIFAAVAAVASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVIL
                     AAASIVVSLFGMFGQTPQGL"
     gene            293..2347
                     /locus_tag="IVW65_002618"
     CDS             293..2347
                     /locus_tag="IVW65_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901460.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLTERHEQIQQELQAQIPKWKREYETQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLAPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKTKEGSSDSTVQSNSSESSESNE
                     VK"
BASE COUNT          998 a          389 c          533 g          578 t
ORIGIN      
        1 gtgcagcaac tagtttaaaa aacaatctga tgatttttgc ggctgtagct gccgttgcct
       61 ccattgttct tggggggatt ttcttcttct ttggccgaag aggagctgaa aacgggaaga
      121 tgcacgtagg ttatgtattg atgggggtag gagttatatt agctgccgcc tctattgtgg
      181 tttcgttatt tggaatgttt ggacaaaccc cacagggact ataaaaaata aatagaagga
      241 gtaaaaagta cgtggctact ttttactcct tttttatgaa aggagaatac tcatgtactt
      301 tacgatttca ggcaatacca aaaaagataa agagttaata aaaaaagcaa cgaagaaagt
      361 gggaaagcgt actgcctttt taccgaaaac aattaaatat tctgtcattg aaacggaaga
      421 acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg ttaaattgac
      481 tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat acgtttttgg
      541 cccactggat tatcaaattt cagaaggttt ttatcctaca ttgatggatt tgatccgcct
      601 tgatgtgttt aaggaaacgg atgaaaccat gagtgtggaa gagtcttatc gaaacgaaaa
      661 actgctacaa gttttagaag aagagttaga gagcgaccaa agaaaagtga gtcaagtgag
      721 tgaagtcgaa gtaaatgatg aagtaccatg ggaacgacca atctttccaa agaaggagtc
      781 aaaagaaaaa gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat
      841 atttgaggaa gagaacgatg taatgggtca attggaggta gatccctttg aagaaacaat
      901 ggaggatgaa gagacggccg aagtagaagt tgatctagtt gaagctaatg aagagccaac
      961 atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagagaa
     1021 aaacgaaaca gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc
     1081 agagcatgaa tggatgaaag aaaaaaataa attattttta aatgagcttt attcgaaata
     1141 tcaattaccc caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg
     1201 taaaggcaca cttttagaac agttagagtc tgttcaatca acaaattggc atgagcttgc
     1261 acaagaagat ttattagcca cttttgaaga acgtcaacaa gaaacagatc aagaaataaa
     1321 tgagtatgcg acggaacagt catctaattg ggagagaaca aaagaggaac taaataaaga
     1381 agaacaacga gtgattgaag aagaagtaca aaaaattaaa gagcgatacg aaaagaaacg
     1441 cgacaatgcg tattccttgt gtatagagaa aattaataac tttactaaaa ctaatcaaga
     1501 agcgttatcg aaagaaaaag aaaacttgtt aaaagaacgt ataaaagaac gaaaagaaga
     1561 gttctaccgc ggtttacgta cagataagct aaagattagc aaagaattaa atgagcaact
     1621 gaataagcta tatcaaacaa caactgaagt tcttacggaa agacacgagc agatccagca
     1681 agaattacaa gcacaaattc caaaatggaa aagagagtat gaaactcaac aacaagaatt
     1741 gaaagaacaa gaagaaaaac aacgacaaga agaaaaagag cgcgaacaat tagaactaaa
     1801 aaaacgtgag cagcatcttg cagaacaaaa agtggcgata gaaaaacaac ggttagagca
     1861 agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg
     1921 acaagcacag ttgaattatc tagcacaacc tcaaccagta gctgctgttc aaacgccagt
     1981 gaacaaccaa gcaaatttag ctccttcacc acagcgttcc tttaacggat ggatggtggg
     2041 atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt ttaatcatat
     2101 caatcaatct tcacaggctg attctgtggc cacaacgacg tcaatcaagg aagaagccca
     2161 agaacaagca agaaaagagt atgaacaaca attggaagaa ttattgaatc gtaccaatca
     2221 gtcaacaaca gaatcaagta tggaaaacag caaagaaagt gattctagta agaccaaaga
     2281 aggatcaagc gattcaactg tacaatcaaa ctcttccgaa agttcagaat caaatgaggt
     2341 gaaataatgc gtgttagaga aacaacgaga cgaaaagaca aacgattcaa tttagaaaag
     2401 aataggtatc tttctcattt ctatcgctat actccttcag gagaagagct accagaaaaa
     2461 catgacaaac gtatttctca aaagattttt tatagtgg
//
LOCUS       DACCWK010000172         2490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCWK010000172 DACCWK010000000
VERSION     DACCWK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.178"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(105..1043)
                     /locus_tag="IVW65_002619"
     CDS             complement(105..1043)
                     /locus_tag="IVW65_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7901461.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1534..2490
                     /locus_tag="IVW65_002620"
                     /pseudo
     CDS             1534..2490
                     /locus_tag="IVW65_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
BASE COUNT          856 a          431 c          398 g          805 t
ORIGIN      
        1 tttttatatt taggaggaat aatcctctga ttttttcata cgttatgtca cctcgtaaat
       61 attaattata ctgaattagc aatttttatc aaataaaact tattttactt ccaaaaccta
      121 aattcacgtt gccaaaaatc aatctgcttt tgcaattgtt tttcgttcgc ttttaaagtc
      181 gatttcatta attccgttaa atcaattgga gatatttctc taatcaattt tttaaattta
      241 gtcttagtat tcttacttag ctttccccac atactttctt catgcaacaa agtataaacc
      301 atagcttgct cattaatttt ttctaaagta gcccacgcag gtttcaagat gtgtaaatca
      361 ttaaaacaat cattccagta atcaaccata tctcttttta attcaacttc tacacgccat
      421 aaatgttcag acacaacttc aacatctgcg ttatctttac gttcttgttt tttattataa
      481 attctaataa atctatcact atcacggaca ccaaaatatt ttgtttctgg cttgccatta
      541 cgaccataaa aaacagtttt cttaactgct ttatcagtca ttgcatagta atcgctcaaa
      601 tcatcttcaa aatcaaaagc taagtctaat cttgtaaaac cgtcatcttc catgtagtcg
      661 ataatatttt gttttaacca aatcatttct tcatgtgtga gtttattggg attaaattca
      721 acacgcatat tacgtctatc ccaagtatct gcttttactt tgtcatattc gatataaact
      781 ttttcttgaa gtgccttagc tttaaacttt gtttgaagta tatcccaaag tcgtatttgt
      841 ggctctacac tcataaagtc agatagcttt ttagcattag ttttgttcaa atttccaacg
      901 attgtcatgg catcaaaact taatgcgggt tgagattttc ccaaagtttg accacttaac
      961 cggctattac ttaaccggct attagagacg gccggttttt ttagtgagat ttctgcagct
     1021 aatttttcat caattttctt cattttttat cgctcctaaa ataatttttg gaacgaaaaa
     1081 aggacgagtg gaatcgcaaa ataaatttcg cctgtatcca ttcgtccatt ttgttttgta
     1141 ttcaaaatca aaaattgata tactaatcat cgaatgaata acaagtgtgg tttgttattc
     1201 cgggcgagaa cgttggtagc gttgctcgtc tttttttatg tctttttttc gttaaaacta
     1261 ttttaaaact aaatatatct taaagcaaat aaattcaagg ggctaaaagc gccccttaaa
     1321 aaccccttgc ctctgaaaag ttttgtgttt cctttccgtc ggtttcaaaa gctaatttac
     1381 tcaaaaaaat agatcaaggg taaatgcact cactgcgttc gcccttgagc tattttattt
     1441 cgcaaatttt aacgcatttg ccgacgaaaa gataaaacac ataaaaatat acgcagatat
     1501 atttaaaacg tcttagaacg caaaatttta cgtttaaaag ctatgcaagg actgtatgaa
     1561 gcaaaattat tgagttatcc aagaacagat acaccattta ttacagagaa cgaatttgct
     1621 tatttaaaag cgaattttgg caaatatagc ggttttttag gacttgatct tgaaatggtt
     1681 caaacagagc ctagaaagcg ttatgtggac ggtagtaagg tacaggaaca ccacgccatt
     1741 atcccaacaa aacaagtacc taccgaatct gcattagcga aaatggacga tttacaacga
     1801 aaaatttatg ctttagtcgt taaaacgacc gttgccatgt ttttacctga ttacttgtat
     1861 gaagaaacca agatacaaac gaaagtagct gatttgctgt ttcaatcgat cggaaagaca
     1921 ccaaagcaag aagggtggaa aattcttttc aaacaacaaa ccaaagaaga aaaagaggac
     1981 gttcaaacgt taccactcgt tatcattggc gaacgtgccg aagttggtgt taagagtgtt
     2041 gaaaaagaaa cgcaaccgcc aaaagctttt acagagggta cattattaac tgctatgaaa
     2101 acggcgaata aaacggttga tgatgaagaa gcaatcaaga ttttacaaga agttgagggg
     2161 attggaacag aagcgacaag agcaagtatt attgaagcgt taaaacaaaa agaatatatc
     2221 caagtgatta agaataagct tgttgtaact gaaaaaggaa aattattgtg ccaggcagtt
     2281 gaaagtcagc accttttaac gagtgctgaa atgacggcta aatgggaaac gtatttaaaa
     2341 aaaatcggta aaagagaagg caatcaagag aactttatta cgaatatcaa aaaattcatt
     2401 gttcatttac tggaagctgt acctaacgat atagaaaaac taaatttttc tgattaccag
     2461 gaacagaaag aaaaagaagc agaaaaaagt
//
LOCUS       DACCWK010000173         2470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCWK010000173 DACCWK010000000
VERSION     DACCWK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.179"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..146)
                     /locus_tag="IVW65_002621"
     CDS             complement(<1..146)
                     /locus_tag="IVW65_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7901462.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINA"
     gene            complement(228..1394)
                     /locus_tag="IVW65_002622"
     CDS             complement(228..1394)
                     /locus_tag="IVW65_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7901463.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(1400..2089)
                     /locus_tag="IVW65_002623"
     CDS             complement(1400..2089)
                     /locus_tag="IVW65_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP7901464.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(2216..2455)
                     /locus_tag="IVW65_002624"
     CDS             complement(2216..2455)
                     /locus_tag="IVW65_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901465.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
BASE COUNT          811 a          465 c          355 g          839 t
ORIGIN      
        1 tgtgcattga tttgatctac gggcatcttt ttttctatat tttgcttaaa cacctctcct
       61 tcatgttttt ggtagctaac atagccaaac acactgcaaa cgacgattaa agcgactcct
      121 aaaaaaccaa ataaccattt tttcatccat ctgcctcctt tcagacaaaa caaaaaacac
      181 tttttcacaa cgaaaaagtg ttttctctta ttactattct atttcattta tctggtggtt
      241 ttatattggt gtgtagccat tgctaacatt aagaatacaa ttggcaccca actgattgtc
      301 gttaatgttt cttccatgaa cgattgacac atgatcgcta aaaaagccca caagaatgtt
      361 agttgtccat ttgtcagctc acgttttctt acgagcaacc ataagaaact aagcaaaaga
      421 ataatcgata gaacaaaacc aatcaatcca tagtgattga cgagtgcgtg atacgtatta
      481 tgattaccta gaatctgatt tcctctatgg aaaaagaaag gtttcatccc tacaaatatt
      541 tgtcctgttt tttcgcctaa tgtttcatag aatttgcgcc aaatttcttc tcgtcctgta
      601 aataagtgaa ctttgtctga cattgcagca aaataagaaa tcggtaaagc tagaaccgaa
      661 actgctgcat acatccaaaa ggacaagtgt gtatttcgaa aaaccttctt aggtaaaatt
      721 tctaagataa tgaagataac aagcgcatat aatgctgctt tcgattggca aacccaaatc
      781 gttgccccag caagaatcaa cgctggtaat gatagcaatc gcatgtacca ttttccaaat
      841 gaactagcaa atcctgtgat aagaagggct aatgtcatta aagaactacc tatcgtattt
      901 gtattgatcc aaatatctga taatttgttc gatggattcc aaatactttg tccttctggt
      961 actggtatct cagtaaatac tcgataaacc gttacgagta aaaagacata gaatactaca
     1021 agatacaagc tacgatcaat tacagtaaat ttcgtccatt ttacaatcaa aataatagca
     1081 ataattgtcg gaacgatcag atcaccatag aaagtatctc ctcggtagct tctaaaaaaa
     1141 gaaaagaaaa ggtaaaaaag aatgcctgct gcaatgaaga atagatcgtt tctctttacc
     1201 ttgtacagat tcaaaagaaa caaacagcct acgaaaaacg tcactaactg tgcagcatta
     1261 tcataaataa ataatatcgt aggaggattt aaccccaccg tccatattaa atatagaatg
     1321 gataccaaca gtaacagaaa aatggcactc attttattga tcaatttttc tacaagttct
     1381 tgaatgtgac tcacttccct tatttattga ttttttctac attgactaat tcacactgga
     1441 tcacatcgca ctttttcata ttatttgctt gaaaggctaa cagtgtgagt agtggttttt
     1501 tacctgcttt tggcaaggat aaaaaggaat tgttttcctt agattgaact acctggctgt
     1561 aatcagaaat ttttcctctt ttggagactt catactgata cttgtaatga gcatcgatta
     1621 gatcaatttc ttctccttca gataaatcag tcatatcgga taataaaacg cttggtgtat
     1681 ctacgttata accagcaaga atataatttc catttcccat ttcagtatct agataataag
     1741 tagaagctac tcctaaacta agcgtgtcgc tattcgctcc ttgaaaaaca gggagaataa
     1801 atcctgtaga agaaaaaaca agttttccag caatttcacc tttgagatcc ttttctaatg
     1861 cctgtttttg attagtaatc agttgtctct tagaaatatc cgttaaactg gttaaattat
     1921 cttgataggt tttagcctgt tcctgaatat ggattgtttt tgttacacca tctacttttt
     1981 cagcgatttt gtccttttga taaccttgat aagcaaaata actaaaaaga agaaaaacaa
     2041 tgattacgcc cacgtaagga ttaaataaag ttttattgtt tttattcatc tatctacctt
     2101 ctatatatga attaatttac ctacatctaa ccaagataaa ccatgttaaa ttttactatt
     2161 ctttttattc aataacaatg ccacatcata attgatcatt ttcatatcat aatcgtcaag
     2221 aaacttctaa taatagttgg caattggaac ctgtttttac ataagttcgc ctcaactgtt
     2281 caaagttttt cattgaacct aacaaacaag ctttctcttt tcctgttatc ttcatatcat
     2341 tgatatattg catgacagat aatcttttaa ggtgtttttg tttcatcatt tttagcaact
     2401 tttcttccat ttctttcgac actttttttc tgttttcgat ttcttcataa ttcatgagtt
     2461 ttcctacctt
//
LOCUS       DACCWK010000174         2444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCWK010000174 DACCWK010000000
VERSION     DACCWK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.180"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            144..2297
                     /locus_tag="IVW65_002625"
     CDS             144..2297
                     /locus_tag="IVW65_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7901466.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          834 a          428 c          541 g          641 t
ORIGIN      
        1 ctttaagtga ttgctgtgaa acaaaaagtt gattttatct tgaaattagg taaagaattt
       61 cttgctccgc ccattttttc ttttttatta agaagttttt gggtacaatg aagcatgaat
      121 gtaaataggc ggaaggatag gaaatgatga aagattttat gaaaaaaata ttaaatactt
      181 cttggttaga aaaaataaga aaaggtgcac aagaacctaa gaacaaaacg tttcgccggt
      241 cccatgttcc ttttcgctta aattttcttt ttttcattat atttacgtta tttgttgcgt
      301 tgattgctag attgggttat ttgcagatcg tgaacggcga agagatggag gcgcgagtga
      361 agtctacttc tacgattacg gtccaagaaa gtactcctcg cggaatgatc tacgactcca
      421 caggaaaagc tttagttacg aataaagcga atcaggccat cacatatacg agaggaacac
      481 aagttacggc taaagatttg cttgaaatcg caacgaagct gaactcgctg atcgatgttc
      541 cggtagatga aaatctgaca gatagagata aaaaagattt ttggctggct gatcccaaac
      601 atttgaaaga agccacaaaa agactttcgg caaaagaaaa gcaattaggg aacagcgaac
      661 aatatagtgc gatggtcaat aaagtcacag ctgatgagat taatttaaac gaggagcaat
      721 taaaaattgc taccatcttt aaacggatga atagtgcata tgaattaaac actgtctata
      781 tcaaaaactc tggtgttact gatcaagagt tagctgttgt ggcagaacgc gcttctgaac
      841 ttccaggagt ttcgactggc acagactgga cacgagaata taatgcagca agttcactga
      901 aaagtattct tggcagtgtg acgactgaaa agcaaggact tccagcagat gaagcagaga
      961 aatacctagc aaaaggttat tctcggaatg accgtgtagg acaaagttat ttggaaaaac
     1021 aatatgaaga tgtcctgcaa ggaaccaaaa cccaatatga agtttccctt gataatgaag
     1081 gaaatgtttc gaatcaaaaa gaaatcttct ctggggaaaa aggttctaat ttaatgttat
     1141 ctatgaatgc agaattccaa tcaaaagtag aagaaatttt gaaaaggaat tatcaaacat
     1201 tgatcaacaa cggcaaagca cagtattcgc ctggtgctta tgcagtggct atgaatcctc
     1261 agacaggaga agtgctagca atgactggtt tcagtcatga acaaggttcc aaagaaatca
     1321 ccgagaatgc tttaggcacg attactagcg cctttgatcc agggtctgta gtcaaggctg
     1381 gtactttgac agcaggctgg gcaagtaacg cgatcagcgg aaatcaagtt ttgattgatg
     1441 agccgattcg tctgcaaggt gcttcagaaa aaagctctgt ctttaatcga agcggacaag
     1501 ttgctcttga tgcagtcaaa gcatcggaat tgtcttctaa tacgtatatg atcaaagtgg
     1561 cgttaaagat gttaggacta gactatacac ctggaatggg actacctagt ttagatgaag
     1621 aagctaaagc ttatcagcaa ttgagagact ctttcaaaga gtttggtctt ggtacaacga
     1681 ctgggatcga tttgccgaat gaatctcctg ggatctcacg ttctgttgat tatatgaaga
     1741 agttcaatgc ggataatggc aaagaatggt atgctccagg taactttacc gatttggcat
     1801 ttggacagtt tgacacatat acaccaatcc aattagctca atatgcttca acagtcgcta
     1861 atggcgggaa acgagtacag ccgcacctag tcaaagcgat ctatggcaat gacgaaaatg
     1921 gaaacttggg tgaagtgaag aaagaaattg gcacaacagt agaaaatacg gtcaatattt
     1981 ctgcagaaaa tatggcgatc ctccgtgaag gattccacca agttgtccat ggtaccgatc
     2041 cttacacaac agccaaacca ttagccagtg cgaaaatgga cctttccgca aaaacaggga
     2101 ctgcagaaac ggttgcggaa ggtcatccag atattgcgac agtcaatagt aatatcgtgg
     2161 catacggacc aacagacaat ccggaaattg ccatcagtgt tgtcttgccg aacttattag
     2221 acgaacaaga ccacataaac ttgacgattg ctaaagaaat aatggacgcg tattatgata
     2281 tgttcatggc aaaataaaaa agaggtcgtg aaaaagccgc ttagtttcga gtatgaagaa
     2341 aaattttgtg aaaatagctt ctcacatttt tgcttaaatt tgacttcata ccgagaaact
     2401 ggcttttgaa caccgtttac tcggcaaaaa aggttgtgac aaaa
//
LOCUS       DACCWK010000175         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCWK010000175 DACCWK010000000
VERSION     DACCWK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.181"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            207..2069
                     /locus_tag="IVW65_002626"
     CDS             207..2069
                     /locus_tag="IVW65_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7901467.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IVW65_002627"
     CDS             2069..>2400
                     /locus_tag="IVW65_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7901468.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACCWK010000176         2306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCWK010000176 DACCWK010000000
VERSION     DACCWK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.182"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1015..1305)
                     /locus_tag="IVW65_002628"
     CDS             complement(1015..1305)
                     /locus_tag="IVW65_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901469.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="IVW65_002629"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="IVW65_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          663 a          434 c          334 g          875 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaata
//
LOCUS       DACCWK010000177         2293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCWK010000177 DACCWK010000000
VERSION     DACCWK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.183"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(51..1598)
                     /locus_tag="IVW65_002630"
     CDS             complement(51..1598)
                     /locus_tag="IVW65_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7901470.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVW65_002631"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVW65_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7901471.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IVW65_002632"
     CDS             complement(2043..2237)
                     /locus_tag="IVW65_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901472.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          643 a          477 c          409 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga agacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACCWK010000178         2269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCWK010000178 DACCWK010000000
VERSION     DACCWK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.184"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            104..1717
                     /locus_tag="IVW65_002633"
     CDS             104..1717
                     /locus_tag="IVW65_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7901473.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          710 a          403 c          445 g          711 t
ORIGIN      
        1 tatattttct gtctattcaa aataaacaat aattttccgg cacaattatt tgctgttttt
       61 taccataaca tagtacgatc aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat
      121 tggcggtgta gctggcggtc cttcatttgc cactcgtttc cgtcgattga atgaagcgca
      181 cgaaatcatt atctatgaac gcggagaaaa tatttcttat gcaagttgtg ctttgcctta
      241 ttatttagga ggtgtgatca ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt
      301 gaaaacaaaa aacaacatcg atgtctttac taaacacgaa gtaacagcaa tcgatccttc
      361 tactaagcga ttaacagtta aagacctatc cacaaatgaa gaaataaaaa cagattacga
      421 taagttgatc atctcttctg gtgctagacc agattatccg gatattcccg gagtttttga
      481 agcagaaaac ggctttgtcc tccgtagtgt gacggatgcg gatcgaatca aatcgttcct
      541 tgaagaaaaa aatccacaac atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt
      601 agctgagaat ttcaagcatc gcggcttaga cgtgacttta atcgatcaat tgccacaagt
      661 cgctttccct tatgatccag aaattgctaa tttagtttat gacaaattgc tgaaagaagg
      721 attagccgtt catttagaaa caagagttac tgagatccgt gataaaggtc gagaaatagt
      781 attatcagat ggctccgtcc tttctgctga tatgctaatt tttgctgttg gtgtttctcc
      841 gaagaatgaa gtggtgaaag caaccggcat acaattatca gatacaggtc agatcattgt
      901 tgatgaccag ttacaaacca atctgccgga catctatgcg attggcgata ttatcgaaac
      961 gactagtgta gtgactggtc agccgatcca aagtatgctt tccagtgcgg ccaatcgtca
     1021 aggacatatg ctggcagata ttttaaatgg tacgcctatg cgctatcgcg gatatattgg
     1081 tgcaggtgtc gcaaaaatct ttgatcatac agcaagttat gcgggaatga cagaacatga
     1141 attaaaagca tcaggcataa caaattataa aactgttttt atcactccct ttgaccatgc
     1201 ctatttctat ccagaagcta caagattaaa tctaaagctg atttttgatg cagatagcgg
     1261 tcgtatttta ggtggacaag catttggaga aaaaggtgtc gataaacgga tgggagaact
     1321 ttctgtagca atcaccggaa acttgacagt ctttgatttg cctgatttgg agttgcctta
     1381 ctctccaccg tattctacta cccgtgaccc gttgaatata gctggttatg tcgcaatcaa
     1441 tcaaatgacg aatatcgtag aaacgatcaa agcaagtgat atacccgaaa acgatttgaa
     1501 agaagcgttc tttttagata tacgtgaacc taataaagca ccttctggca gtatctcggc
     1561 aacaaaaaat attccaatga atgagttacg agatcgaatc aatgaaatcc caaaagataa
     1621 aaaagtttac attactttca gaagaggatt gaatacttat acttctgccc gaatcttggc
     1681 aggtttgggt atcaaagcga ttttgattga agaataaaaa aacgaccgcg gtgggtagcc
     1741 gcggtctagg agttattttt acttgaggag taaaaatgaa aaagtatata gggttgttgg
     1801 ttggtatgta tatagaatag aagataaatg tgaagaaatt gtgcattatt tcttcacatt
     1861 tattaggtta tagtttaaga aagtttaaac attgcgcaat ttaattattt tagctgtttc
     1921 ttttcataaa aaaacatgtt gtgactctga gacaaatcat agtccatagt gatgtctcta
     1981 gagtaactac gcctaatatt acgaggcatc atattaatct atcagcacta gtacctaaac
     2041 tagcatccct ttcgaatatt ttttcttata taataaagtt tgctttcact cctctaatct
     2101 caccaaatcg actggcttgc gttgtgtgat actaggaact gattggttta gaacgacttt
     2161 tctattgtaa tcatattttt cgccatcctc gttgtcttct aatccttcaa tgaatgatcc
     2221 ctaatgtgtg tattaaataa tttaattttt ttgttcctat ttatttaac
//
LOCUS       DACCWK010000179         2241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCWK010000179 DACCWK010000000
VERSION     DACCWK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.186"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            316..570
                     /locus_tag="IVW65_002634"
     CDS             316..570
                     /locus_tag="IVW65_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7901474.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IVW65_002635"
     CDS             571..888
                     /locus_tag="IVW65_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7901475.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..>2241
                     /locus_tag="IVW65_002636"
     CDS             1447..>2241
                     /locus_tag="IVW65_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901476.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGD"
BASE COUNT          798 a          321 c          424 g          698 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga t
//
LOCUS       DACCWK010000180         2190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCWK010000180 DACCWK010000000
VERSION     DACCWK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.187"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            29..208
                     /locus_tag="IVW65_002637"
     CDS             29..208
                     /locus_tag="IVW65_002637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901477.1"
                     /translation="MEKDIYKQLTQQIQKEKEKGLLDHLDELELKADEINKNRMSAKE
                     KGIKLKDSSLQSDKS"
     gene            694..834
                     /locus_tag="IVW65_002638"
     CDS             694..834
                     /locus_tag="IVW65_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016373449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAP7901478.1"
                     /translation="MSKKKRLTITLSEQVLEYLDEVAKKKGLSKSALITIALEEYKKG
                     QK"
     gene            900..1640
                     /locus_tag="IVW65_002639"
     CDS             900..1640
                     /locus_tag="IVW65_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HAP7901479.1"
                     /translation="MTVRITKARHFSFLLYPDSIPNDWKEKLESLGISMAVSPLHDMD
                     EKKDKEKWTIDDVVRNGKHYKKPHYHVIYIARNPVTIESIRKKIKRKLGNNSVAHVEI
                     IDSIKGAYEYLTHESKDAKAKNKHIYDKKDILYINDFDIDRYITLDESQKRELRKTLT
                     NIIDMYGVVNAKELRAFLNWRGTEFGVTNLGDVEDVLSANGSLFRFYFDGNYQNGFRP
                     QYAKRINSETGEIISGVDELISKTEEGE"
     gene            1643..1861
                     /locus_tag="IVW65_002640"
     CDS             1643..1861
                     /locus_tag="IVW65_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901480.1"
                     /translation="MTKNVNDLFEEYYDNHNLDESRYSHFSKKQLVIEAEYMHDALQN
                     ILKYLDDGGTDLNAIRGEVMDGIYESRI"
     gene            complement(1917..>2190)
                     /locus_tag="IVW65_002641"
     CDS             complement(1917..>2190)
                     /locus_tag="IVW65_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901481.1"
                     /translation="NTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNS
                     KVYQEVKEKIEAKLNPSNRNRPKNHQKGTNKTVTSSNFDPTKFLKR"
BASE COUNT          783 a          278 c          468 g          661 t
ORIGIN      
        1 aataatagct tttaaggagg tgatggggat ggaaaaagat atttataaac aattgaccca
       61 acaaattcaa aaggaaaaag aaaaaggctt gctagatcat ttagatgaat tagaactaaa
      121 agctgatgaa atcaataaaa acagaatgtc agcaaaagaa aaaggcataa aacttaaaga
      181 ttcgtcgttg caaagcgata aaagctagat aacgtgcggt caacggtaaa tcgaattaga
      241 gacgcttaga gaggctaata agccctctta gcgtctcttt gaggctttaa ggagttgacg
      301 gctataaaca aaacccaaaa gctggaaaca caagaaggtg accataggga acgttcttga
      361 cgccttaacg gcgagtggaa agtttttggg gtgccttttt tcaaaaaggt acggtcttga
      421 tttgccgagc ggtagcgctg gaaaattttt ggaaaaaatt tggaatttgg aaaatctggg
      481 gggctactac gaccccccat agtgtgccga gtgccaattt ttgtgccaaa aagtgcttta
      541 tccttactgt accaatggat agagcctttt ttttcgaccg ccatttgacc gccgtttcga
      601 ccgccgtttt taaaaaaata cgcttttagt tgcatttaat gcgtaatatg tggtattctt
      661 tttaagaaga taaataatga aaaggagaat gacatgagta agaagaaacg tttgacaata
      721 acgttgtcag aacaagtttt agaatatcta gacgaagtag ctaaaaaaaa gggtttatcc
      781 aaatctgcat taataacaat agcgttagaa gaatacaaga aagggcaaaa atgagcataa
      841 aaaaagagcg ctcccgacta aaagaccgct cactcattaa ttgataaggt gattatatca
      901 tgactgttag aattactaaa gctagacatt ttagtttttt gttatatcct gattcgattc
      961 caaatgattg gaaggaaaaa ttagagagtt taggtatttc aatggctgtt agtcctttac
     1021 acgatatgga tgaaaaaaag gataaagaaa agtggacaat tgatgatgtt gttagaaatg
     1081 gtaagcacta taaaaaaccg cattatcatg ttatatatat tgctagaaat ccagtaacga
     1141 tagaaagtat tcgaaaaaaa atcaagagaa aattgggaaa taattcggtt gctcatgttg
     1201 aaataattga ttccattaag ggtgcttatg agtatttgac tcatgagtca aaagacgcga
     1261 aagcgaagaa caaacatatt tacgataaaa aagatatttt gtatattaat gattttgata
     1321 ttgatcgtta tatcaccctt gatgaaagcc aaaaacgaga gttaagaaaa actttgacaa
     1381 atattattga tatgtacggt gtggttaatg cgaaagaact tagagcattt cttaattgga
     1441 gaggtacaga atttggtgta accaacttag gagatgtaga agatgttttg agtgcaaatg
     1501 gtagtctgtt tagattttat tttgatggga actatcaaaa tgggtttaga ccacaatatg
     1561 caaaaagaat aaattctgaa actggggaaa ttataagtgg agttgacgaa ttaataagta
     1621 aaactgaaga aggagaataa gaatgacaaa aaacgtaaac gatttgtttg aggaatatta
     1681 tgataatcat aatttagatg aaagtcggta tagtcatttt agtaaaaagc aattggtgat
     1741 tgaagctgag tatatgcatg atgctttgca aaatattttg aaatatctcg acgacggtgg
     1801 aacggatttg aatgcgattc gtggagaagt catggacggc atatatgaaa gtaggatatg
     1861 aaaaaataga atattgggaa ataaaaaaca gataggaata actcctatct gtttttttat
     1921 cgttttagaa atttagttgg gtcgaaattg ctagaagtga ctgttttgtt tgttcctttt
     1981 tgatgattct ttggtcgatt tcgatttgat ggatttagtt tagcttcgat tttttctttc
     2041 acttcttgat atactttaga atttgtgcca ataagaacgc ctgtatcttt tagatataaa
     2101 tcagcgaatt ctgcgatgat ttcagtcatg atggaaattt cttttcccat agcatttagt
     2161 tttgcctgat tgctggtagt ggtcgtgttg
//
LOCUS       DACCWK010000181         2167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCWK010000181 DACCWK010000000
VERSION     DACCWK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.188"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            34..717
                     /locus_tag="IVW65_002642"
     CDS             34..717
                     /locus_tag="IVW65_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7901482.1"
                     /translation="MSRSQKGLAGAGEWETLKKMLPDFKGKRVLDLGCGYGWHCIYAM
                     ENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDFPEESFDVILSSLAFHYV
                     ADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPVDNYYYEGK
                     RTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRRPMM
                     LIVSAKKKM"
     gene            750..1658
                     /locus_tag="IVW65_002643"
     CDS             750..1658
                     /locus_tag="IVW65_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7901483.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1655..2135
                     /locus_tag="IVW65_002644"
                     /pseudo
     CDS             1655..2135
                     /locus_tag="IVW65_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          748 a          312 c          492 g          615 t
ORIGIN      
        1 gatgataata tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct
       61 ggtgcgggag aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg
      121 cttgatttag gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc
      181 tctgtagtag gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat
      241 tttccacaga ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc
      301 tttgatgtaa tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata
      361 aaaaagatat ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct
      421 gtttttactg ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat
      481 ttcccggtgg acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag
      541 gttacaaaat atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt
      601 ataataaatc agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg
      661 gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa
      721 tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag
      781 tactttcttt agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg
      841 caaatattaa tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg
      901 atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga
      961 tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta
     1021 tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata
     1081 attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa
     1141 gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg
     1201 atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta
     1261 aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa
     1321 tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata
     1381 agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga
     1441 tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg
     1501 tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa
     1561 caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca
     1621 catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca
     1681 cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag
     1741 gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag
     1801 ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa
     1861 tactgataag ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg
     1921 ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact
     1981 tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata
     2041 attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
     2101 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
     2161 tcgtctt
//
LOCUS       DACCWK010000182         2152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCWK010000182 DACCWK010000000
VERSION     DACCWK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.189"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(198..377)
                     /locus_tag="IVW65_002645"
     CDS             complement(198..377)
                     /locus_tag="IVW65_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901484.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(758..1465)
                     /locus_tag="IVW65_002646"
     CDS             complement(758..1465)
                     /locus_tag="IVW65_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901485.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            complement(1458..1895)
                     /locus_tag="IVW65_002647"
     CDS             complement(1458..1895)
                     /locus_tag="IVW65_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901486.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            complement(1910..2113)
                     /locus_tag="IVW65_002648"
     CDS             complement(1910..2113)
                     /locus_tag="IVW65_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901487.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYEIEYWLTKLEN"
BASE COUNT          590 a          433 c          336 g          793 t
ORIGIN      
        1 aaatcataat atgtttaaga aaacaaaatc tttagaatca ggtaagaaaa aaattgatct
       61 aaaaataatc tcttctggat actttaaaga tttcatagtt attgatacaa aatggaataa
      121 aaaggacata gaaaatattc taaatagtag gaacaattaa gtgctatcac ttttgtttct
      181 tcaaatgaaa agtgacgtca cttttttagt gctggtatat caaatcctgc tttttcttgc
      241 gcgatcaata gatctaacgc aatacacata atcttagatt cactttttcc gtacttctgc
      301 tttagttgct gcaattcttt ttgtctcttt tcatctaatg tcacgggtaa ccgtttattt
      361 cctattgcaa ttggcattag tactctctcc tttcatcgaa ataaccctat gaaaaatatg
      421 gctgcaatca tcttgaatag tagactcaca agggctaata caaatcgcca aaagatcaaa
      481 caaaatacca gtattcctaa gaatactacg aagtttaaat gtacagctgc agcatgacgc
      541 ataagtatct cgccaacaaa cagtacgaac aatagaggaa tgattttatt catatggatc
      601 tccttctttc tcttaggtta tcgggctttg cccgcttatt tcgcaccacc taagcggcgg
      661 gggcggttgg agcgattggt gcgaaataag aaccattccc ttaatttcaa ggttttttgt
      721 tcttatttca cacatcctca cttttcggta taaatggtta tttacctttt ttcctttcaa
      781 atcgttgata tggcattcgt ttacggtcaa gttcattttg gatttcttct gtatcgtcgt
      841 aagtgtaggt gtctaaaaaa tcagttaaag tcgtgtacca gtcgtcgcct gggttatcta
      901 cttcttctac atattccctc aaaaatgctt cgggtacagc gagttcataa tccattccca
      961 ttaacacgat agattccgtc ccacacgtca aaaacctagt aaatacaagg taataatcgt
     1021 ctaccgtcgc atttttgctg tttagctctt ccatttcttc ttccattaat tccatgctct
     1081 cgaaaattga ataatattct gattctgagt aatagtgttt cctacaaatc gtgtagctga
     1141 caaagttttc gaccagctca ctcctttcta ggtacacatt catgatgtat ttttctgttt
     1201 ctagtcgtag aagctctgtt ccttttttta ccgtttcagg acgacgttta tacacgtcta
     1261 aatgattatg gctgactgtg accttcgttt gacttgtttc gctttccata tacaactgtt
     1321 cttccactct ttgaataaag tacttcattt cttccataca actaaagtct ttttgaatcc
     1381 gtctgattac ttcattcaag tgttctttgt ttgttaaaaa ttgcgaaaca tcttctgttg
     1441 ttagctgata aaccacctta ttcattatct ttctcctccg attcaatcgt ataaaatgca
     1501 ccattttttt cgatactttc aaaagtatct agccagtcgc tagccattgc tttagctttc
     1561 ttaatctgtg cgaattttgc ataagtcagt tctaaggctt gatattcgga aggtttgata
     1621 gtgcttggtt tgacacctac ttccaagcat agtaaggctt ggaaaaacca ttttccattt
     1681 tgttttttca gttcttccag tgttcgatag tacgtcgcaa tgagattcat ttcgacacct
     1741 gattcaatca tttcttccac tgcgactgac aatttttcct tgtaatgttt tttgggtaca
     1801 cgtgtttcat ggtataggaa cgtcatatcc tccatttttt gtgccatttt atccaaaggt
     1861 aatttgacaa attcgtcata gtaattttga gccatatttt ttctcctttt tagttctcta
     1921 atttggttaa ccaatattcg atttcataag tttccatgtt ttcttgataa tgcagttcat
     1981 atcctgcaat cttcactagg tcttcaaata gcatagtcgg tgcatcctct actaaagggg
     2041 tttctatcgt ccagtaccgc ggaataagta aagcttctgc tactttttca gaaatgactt
     2101 ctttgtccac caaaatttga acgacttctt ttacagtcaa taatttgtgt tg
//
LOCUS       DACCWK010000183         2107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCWK010000183 DACCWK010000000
VERSION     DACCWK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.190"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(208..303)
                     /locus_tag="IVW65_002649"
     CDS             complement(208..303)
                     /locus_tag="IVW65_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7901488.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            978..1697
                     /locus_tag="IVW65_002650"
     CDS             978..1697
                     /locus_tag="IVW65_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7901489.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            complement(1834..>2107)
                     /locus_tag="IVW65_002651"
     CDS             complement(1834..>2107)
                     /locus_tag="IVW65_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901490.1"
                     /translation="NTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNS
                     KVYQEVKEKIEAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          662 a          336 c          404 g          705 t
ORIGIN      
        1 aatacgagct ctcagagcga ctttatttgt aaatgttgca tataagaaat aattacggat
       61 tttagacttt tgtaggatat ttttgaaata aatatttatt tctgttataa tttgattgca
      121 aataagtgaa cacgaaacac acgaaagccc cagagaggtc ggattctctg gggctttttt
      181 tgtgggctga gtcgccttaa ttaagtttta cttcttgttt cggttattca accagtaact
      241 gaacaaagcg cttaaaagcc caacaagaag ggagcaaata aagttagtga acacgaaaca
      301 cacctccttc cagtcgcaag actgtcggat aggcgacaat caattataca ataaaaaagc
      361 aaaaataagc ctaagttttt tattgccttg gggttgttag ggcttgccct gaccgcctgt
      421 aaggcgcttt agaacgaagg acatagtttt tatcgaatct tgtcttagaa cggcttagac
      481 gagctctcag gacgccacaa aggagtccct aatgaattat ttttggcgaa ggagcctaaa
      541 ggcgactgct gcaagtaaaa tgtgagagcg tagcgatcac tccttcttac agcgaccata
      601 gggagctaga ttggcagttt aatatcatgc gcgaacctgt ttattttaaa aagtgggtga
      661 ttttttggcg ggaaattttg ctcgcgcgcg cgcgtttatt ttatttattt tttaaagatt
      721 ttttagaacc ctttttaggg ggcagttgag ccttactctc ccaacggata cagaagggta
      781 ctaacaactt tttatctgag tactaacaac tttttatctg agtactaaca actttttatc
      841 tgagtactaa caacttttta tctgagtact aacaactttt tatctgagta ctaatatcct
      901 tttgtataat aacaaccaac ctatttacga gttggttgtt aaaggttatt atttatttgt
      961 aaggggggaa gaaaggaatg gatgaaaaaa cggaattaac agtcaaatat caaaatgaat
     1021 tgaatttagt accgctgaaa aatttcaatg ctaagcagat ggatttattt tttgcattat
     1081 gcgctcgtat gaaagatcgt ggtttgaaaa aagttagatt ttctttcgag gaattaaaag
     1141 agttaagtga ttataagatg actgctacaa aagcctttgt cggtgattta gaaaagctct
     1201 ataaagatat gcttaatctt acttaccgta ctgaagatga ggattctatt cgctactttg
     1261 ttttgtttac tggttttgaa atcaataaaa caaaaaagtt tgttgaagtc agtgttaatc
     1321 cggacctaga atatattatt aatggtttaa cagctgaatt tagtagattt gaattatctg
     1381 ctttcacgtc gttgcgttct acgtatgcaa aaactctatt ccgactgctt atgcagtacc
     1441 gttctactgg ttattatgta gttcgtattg aagagtttag gagcttaatg gatatcccag
     1501 aatactatca aatgggtaac attgatcaaa aagtattaaa tcctgcatta cgagaactaa
     1561 aaaaatactt caatgatctt aaagtcacaa aaataaaagc aaaaaagggc aataaaatcg
     1621 ccaaattaga atttactttt tcaagcctaa cgtcagattt gccaaagatt tctctgcgta
     1681 actggttaga agaataaata aaaaagaact cgcccccctt acattaagca agtagacaag
     1741 tcccttacaa ccgactaaga ttgctagtac attctagcat attgtatggg gcttctaaat
     1801 aaaaaacaga taggaataac tcctatctgt tttttatcgt tttagaaaat ttgttgggtc
     1861 gaaatttcta gaagtgactg tttgactggt ttctttttga tgattctttg gtcgatttcg
     1921 atttgatgga tttagtttag cttcgatttt ttctttcact tcttgatata ctttagaatt
     1981 tgtgccaata agaacgcctg tatcttttag atataaatca gcaaattctg cgatgatttc
     2041 agtcatgatg gaaatttctt ttcccatagc atttagtttt gcctgattgc tggtagtggt
     2101 cgtgttg
//
LOCUS       DACCWK010000184         2092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCWK010000184 DACCWK010000000
VERSION     DACCWK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.191"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>2092
                     /gene="acm"
                     /locus_tag="IVW65_002652"
     CDS             <1..>2092
                     /gene="acm"
                     /locus_tag="IVW65_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7901491.1"
                     /translation="MLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNI
                     KVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQ
                     VVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSS
                     SSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQI
                     HLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIE
                     NPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDT
                     EIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDF
                     DPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRAST
                     NNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKI
                     ENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDW
                     RYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPW
                     KNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTS
                     SKKSSFAVIIGLLIV"
BASE COUNT          820 a          342 c          407 g          523 t
ORIGIN      
        1 aatgttattt attattacta atataacatc actgattcct gttcatgtct atgcggatgc
       61 aggcagagat atcagcagta atgtcacttc gttgaccgtg gacccaacga atattacaga
      121 tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg
      181 tgattatctt tggatcaatt ggccaagtga aggaaatatt cgcggtgaag gattccaaaa
      241 agaaatccca ttaatgattg aaaataaaaa tgttggaact ttaacggtca gaaaagattc
      301 tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac tcagtagaag gttgggggca
      361 atttgagata caggccagaa acgtaaccga taccggtgaa gaaaatacgg gaccattcac
      421 cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca aagccagctt ctggttcttc
      481 ttctagcgtt ttttattata aaacaggaga catgctacct gaagatacta agcacattcg
      541 atggtttttg aatattaaca ataacggcac ttatgttgaa caaccagtta aaatcagtga
      601 tgaaatccaa agtggacaaa gattagatcc aagtacgttt gaaattaatc aaatacactt
      661 aggtgaacag aaggtttata gaggagaaga gggaatccaa caatttttac aagatttccc
      721 cagcgctaca tttaattttt cagtaactga taattacatt gaaataacta taccaaaaaa
      781 ctttgtcaat cttaggaaaa taatggtgag ttataagacg atcatagaaa acccagaaca
      841 aataaatttt gagaatcatt cggaagcttg gtttaaagaa tttaataaac ctgctgttga
      901 tggagaaagt ttcaatcata cagttaaaaa tatttcagct tctggtggag tcaacggtac
      961 agttagaggc gaattgaaga ttttcaaata tatcaatgac acagagattg gtatcccaaa
     1021 tgtgactttc gaattaagac gagcagatga acagcctatt caaggacagt ctagtatcct
     1081 gttgacttcg aatgaacagg gagaagctag tatcaaaggc ctgcaagtag gagactatgt
     1141 tgtaaaagaa aaagaagcac ccaattggat tgattttgat ccactaagtt caaatgaact
     1201 caaattctcc ataaacgaaa atgatacaga gggagtcagt ttacctattt ataacaagaa
     1261 aaaggttacg aacatcacag caactaaaat ttggaacggt ggaaccactc caagaccgag
     1321 tatttatttt aaattattca gagcaagtac aaacaattgg gaaccagttc ctgatgcaga
     1381 aacaaaacga ttagacaatg gaataacttc agtgacttgg gaagatatcc aacagtatga
     1441 tgatagtgga aacgaatata cgtttaaagt gcaagaagta gatcaaaacg gaaatgatta
     1501 tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg agaataaaga
     1561 agtcatttca gtctcaggac aaaaaacatg ggaagacaat gagaaccaag acggcaaacg
     1621 accgacgacg atcacagtaa atctattagc cgacggtcaa ccgatccaac acaaagaagt
     1681 aagtgaaaaa gatgattggc gctatcgttt cacaaatctg ccaaaataca gagacgggca
     1741 agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa tacagtacga cgattgacgg
     1801 gtataacatc aaaaacagtt atacaccgga aaaaacaaat atatctatac caggtagcca
     1861 tataacaccg tggaaaaatt ttccagagaa gacagagaga gagaaaatta gaaatctatt
     1921 ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa gaagcaatta
     1981 tattagtaag agagctgtac ctaaacagat aacaaataaa tcaaaaagca ttcttcctaa
     2041 aacaagtagt aaaaaatcta gttttgctgt aatcattggt ttattgatag ta
//
LOCUS       DACCWK010000185         2090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCWK010000185 DACCWK010000000
VERSION     DACCWK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.192"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..637
                     /locus_tag="IVW65_002653"
     CDS             <1..637
                     /locus_tag="IVW65_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HAP7901492.1"
                     /translation="KKLIKWLILAVIGIYILNVITKARDGGGLSKLTLKDFTSVFDSD
                     DSPLVDSFFEMGGTMSIITFFLISGNGIYPFANTYLTSLLGSISTRMLSMFGIKFVLL
                     ADWFSQDYLGITWGTGFSMIAEAYVNGGYIGGIVYIFIIGIVIGKILSNSIDNQDIEN
                     HPMKMFLAITTANIIMGFPRAASYLIVKNFVYGVLIILLINFFLQQFNKRL"
     gene            683..>2090
                     /locus_tag="IVW65_002654"
     CDS             683..>2090
                     /locus_tag="IVW65_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HAP7901493.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAV"
BASE COUNT          676 a          288 c          367 g          759 t
ORIGIN      
        1 taaaaaatta ataaagtggc ttattttagc tgttatagga atatatattc taaacgtaat
       61 aacgaaagca cgtgatggtg gaggactaag taaacttact ttaaaagatt ttacatctgt
      121 atttgattca gatgattctc ctttagttga ttcttttttt gaaatggggg ggactatgag
      181 tattataact tttttcctaa taagtggtaa tggaatatat ccctttgcta atacttattt
      241 gacctcactc ttaggctcta tatcaacaag aatgctatca atgtttggaa taaagtttgt
      301 tttattagct gattggtttt ctcaggatta tttaggaatc acttggggga ctggattctc
      361 aatgattgca gaagcctatg tcaatggtgg atacattggg ggtatagtct atatttttat
      421 tattggtata gtcatcggta aaatcctttc taatagtata gacaaccaag atattgaaaa
      481 tcatcctatg aagatgtttt tggctataac tacagcaaat attataatgg gttttcctag
      541 agctgcttca tatttaattg tgaaaaattt tgtgtatggt gttttaatta tattgttgat
      601 aaactttttc ctacagcaat ttaataaacg tctttaatat ttaagcttaa taaaaagata
      661 aaacaagtca ggagttgcaa aaatggaaag aactgctcgg ctcaaaaaaa acactatttg
      721 ggctctggtg tttcaaataa cttctattgt ctgtggtatg atcttgctac gtttgatatt
      781 aacaaagtac ggatccgagg ttaatggaat gattagctct attaaccaat ttctacaaat
      841 tatttctttt ttacaattag gagtaggcgc tgtaatgcaa tcaactctat acaaaccact
      901 agcagaaaag gataatttaa tgataagtca aatcttagct tctggaggga agttttatcg
      961 gcaaattgca tatattctac tagtatacgt gggtttgtta cttgcagtct attatgttct
     1021 cgttcctaat gaatttggga gatcatatac aatggcaatg attgcaacat taagtattaa
     1081 ttcatttgct caatattatt ttggtattgt taatactttg ttattaagtg ctgaccagaa
     1141 aatttatatt acgtatattg cacagacaat agcatttatt tctaatacag ttgcatgtgc
     1201 tttattaatt agtctgaaca cagagatcct tatggtaaaa cttattactg ctataattta
     1261 tcttgcttgt ccaatatatt atagatggta tgtggatcga aactataata taaagaggaa
     1321 tgtgtcatat gataaggaac cgatccctca aaaatggaat ggggttgcac aacacgtagc
     1381 ttctgttgta ctagatagta ctgatgttgt agtgttatct atattttcta acctgatata
     1441 tgtatctata tataatgtgt acaatttagt aatatacgga gtaaaacagc tattcatggc
     1501 tatgacaaat ggcgttcagg ccttactagg ggaactttgg gtcagaaaag aaatagaagt
     1561 tctttacaaa acatttgcat ggttcgaatg gattgtacat actttgacag tttttatttt
     1621 tggctgtacc ggtatactaa tcgttccatt tattcaagta tatacaagag gagtaacaga
     1681 tgctaattat aatcaattat cattcgcagc tttacttgta actgctaacg cacttcactg
     1741 tctgcgtaca ccttatcatg taatgataaa agcagctggt aaatataaag aaactcaaaa
     1801 tagttttata attgcttctt tgttaaattt aggtttatca ttaattttgg ttaaaccact
     1861 agggctagtt ggagtagcat taggatctct aatttcaatc acatatcata cactttggag
     1921 tgcaaattat aattcaaaat atttgcttaa atggccgatg aaaaaattct ataaacagtt
     1981 atttgtaaac atcttaactg tagttctttc ttttggggcg acatcacaat tgaaaatgat
     2041 gtcaatcaca tatttggatt ggattgttct cgcaattaaa tgtgcagtta
//
LOCUS       DACCWK010000186         2074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCWK010000186 DACCWK010000000
VERSION     DACCWK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.193"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(524..889)
                     /locus_tag="IVW65_002655"
     CDS             complement(524..889)
                     /locus_tag="IVW65_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901494.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(965..1210)
                     /locus_tag="IVW65_002656"
     CDS             complement(965..1210)
                     /locus_tag="IVW65_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7901495.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1280..1534)
                     /locus_tag="IVW65_002657"
     CDS             complement(1280..1534)
                     /locus_tag="IVW65_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901496.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1698..2021)
                     /locus_tag="IVW65_002658"
     CDS             complement(1698..2021)
                     /locus_tag="IVW65_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901497.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          773 a          357 c          365 g          579 t
ORIGIN      
        1 tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac tatcaatatt
       61 aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga tcacaaaatc
      121 aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact acagaggctt
      181 aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat gtagtaatcg
      241 aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa gcaatctgtg
      301 ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca ggagatgttc
      361 atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta gttttgcacg
      421 tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga tggttaaagc
      481 tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta agataaattc
      541 ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt tccggataaa
      601 tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat ttgccacgat
      661 acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt tcatccatga
      721 caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa tccacagtag
      781 catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt tgtcctaaaa
      841 gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata gaaatgtctc
      901 ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc aaggctggaa
      961 aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca acggaaatat
     1021 cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct ccttcttcgt
     1081 acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact tgagataaag
     1141 atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa atagcttctt
     1201 cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta tagaaagcta
     1261 cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa agaatgacga
     1321 tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg ggttcaaagt
     1381 agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa atagctaaat
     1441 aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt gcgaccgcag
     1501 gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa cgattcatat
     1561 aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa ataaaagaag
     1621 aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca ggaactgttc
     1681 cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa gtaattcaat
     1741 acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat cccatagcga
     1801 atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt tcctttatag
     1861 ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat tccaataaac
     1921 agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc aactaaatcg
     1981 aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa ataaaagaat
     2041 aacatttttt ctaaaataag caaataaaaa ttaa
//
LOCUS       DACCWK010000187         2048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCWK010000187 DACCWK010000000
VERSION     DACCWK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.194"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(103..921)
                     /locus_tag="IVW65_002659"
     CDS             complement(103..921)
                     /locus_tag="IVW65_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7901498.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1082..1771
                     /locus_tag="IVW65_002660"
     CDS             1082..1771
                     /locus_tag="IVW65_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7901499.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            1785..>2048
                     /locus_tag="IVW65_002661"
     CDS             1785..>2048
                     /locus_tag="IVW65_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7901500.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAG"
BASE COUNT          707 a          302 c          348 g          691 t
ORIGIN      
        1 ccaccgctat tagtaaaaaa taagctatca agtatgctct catcccaaga attgcctact
       61 tcaatcgtta aaaaccgtta ggtttagcta agtgggggta gttcactcaa tacttttatc
      121 aataatagag cgcaaaactt tttctcttaa ttcaactgaa tcaatattat tgttttgttc
      181 atagccatga actaatagat cacaaatata aagttgtgat atttttccag ctaaagatcc
      241 accattaaga aattcttcaa tggctgtttg taaaactaag tctgcagact ttcctatagg
      301 tgaatgaata tagttagtta tagcaataat ttttgcgcca ttttttttag ctattttcaa
      361 tgaatcataa gtatctttcg ttttcccaga taaagaaaaa atgattacta aatcattaac
      421 tgttaataaa gaagcgactt gggcttgaaa atgagggtct agtacagctt ttgcttgaac
      481 tcctacgcga agaaacatat tttctaaatc aagactagta tttccactag atcccactcc
      541 aaatatatat atgttttttg attgagtgat cattttaatg gcttcattca atttttcttc
      601 gtctaaaagc ctaatagtgg aatagagagc ttctgttaaa ctattagcaa tttcatcata
      661 gtactttaca ctagtcggat gttctttttt ttgactaaag tcttcctttg caatttcaat
      721 ttttaaatca gaaaaaccag aaaaaccaat tttttgacaa aagcgaataa tagtagcatc
      781 gccaactttt gtgtttttct taatatcact catcgtacta taaataatag ttttcccaga
      841 atttaaaata aaatcagcga cttttttttc agacttcgtt aaaaatggat aattttcttt
      901 aattaaatag atataactca tacaaactcc tctacaattt caacttttct ccattatact
      961 ccaaaataat aaaaaaaact cctaaatact ccatttttat ctttactttt atgaaagcgg
     1021 atgctatatt gtttttggag ttattatcca aaaataataa aaaattggag cgtttaggat
     1081 aatggataaa aagaggtttt tagatcttat ttcaaaaggt ttgattgttt catgtcaagc
     1141 attacctggt gaaccatttt acaaagagga agaaagttta atgccttatt ttgctttggc
     1201 tgctcaaagg gctggagcag ttggtatacg tgcaaattct gttcgagata ttattgaaat
     1261 tagaaagtgt gtatcattac caatgattgg aattattaaa agagattatc caccagaatc
     1321 tccattcatt actgccacta tgagagaagt agatgaatta gtagaaaccg gtgttgaggt
     1381 aatcgctctt gattgcactc ttagaaatcg acatgatgga ctatctataa attcttttat
     1441 tactaaaata aaagaaaaat atcctcatca gttatttatg gcagatattt ctacatttga
     1501 agaaggcaaa aatgcatata aagcaggaat agattttgta ggaactactt taagtggata
     1561 tactgaggaa agtaaaaaac aagatgggcc agatatagat ttgattaaaa aattggtaga
     1621 agaagggatt tgtgtaattg ctgagggaag gattcatact ccagaacaag caaaagagat
     1681 aaaaaaaata ggagttgcag gtattgttgt gggtggtgct attactcgac cacaggaaat
     1741 tgcatcaaga tttgttaaag cattaagtta gggggagaaa taaaatgttt aaaaaatttt
     1801 cacaattagg acgcgctttc atgttaccaa ttgctatatt accagtagcc ggattactat
     1861 tgggactggg aggagcgtta acaaatgaga gtgctataaa tgcttatcca tttttagatc
     1921 aaccgtggtt gcatacaatt ttaagtatta tgagttatgc tggaaatgca gtttttgcaa
     1981 acctagcact aatttttgca attggtattg ctgtaggact tgcaaatgga gataaaggaa
     2041 cagctggt
//
LOCUS       DACCWK010000188         2007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCWK010000188 DACCWK010000000
VERSION     DACCWK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.195"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..694
                     /locus_tag="IVW65_002662"
     CDS             <1..694
                     /locus_tag="IVW65_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7901501.1"
                     /translation="PDVIIGRKERPVSCGMRTLYRLFSKGIFDVDTLPMKGKRKPNGH
                     QEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEDDTIVGIHHKSAVITLVERLSKVIITI
                     KPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGT
                     PSQRPLNENSNGILRRNGLPKSMDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFL
                     SYVQEAFYSSLI"
     gene            complement(853..1329)
                     /locus_tag="IVW65_002663"
     CDS             complement(853..1329)
                     /locus_tag="IVW65_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7901502.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(1342..>2007)
                     /locus_tag="IVW65_002664"
     CDS             complement(1342..>2007)
                     /locus_tag="IVW65_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7901503.1"
                     /translation="MYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWL
                     YVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWA
                     FIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIES
                     VEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILG
                     KDN"
BASE COUNT          699 a          374 c          295 g          639 t
ORIGIN      
        1 gcctgacgtc attatcgggc gaaaagaaag gcctgtttca tgcggtatga gaacacttta
       61 tcgtttattt tctaaaggaa tatttgatgt tgacacacta ccgatgaaag gtaaaagaaa
      121 acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa tccatgatcg
      181 acctgataat tatcctgatt tcaattctga gtttggtcac cttgaagatg atacgatcgt
      241 tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta aagtcattat
      301 cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta atcaatggtt
      361 ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa aagaattttc
      421 taactggaaa gccattagta accaacatga tattgatata tattttgcgg accctggaac
      481 accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta atggactgcc
      541 gaaatcaatg gattttagag aagtgaatca gacatttatt tccagtgtca gcaatcaacg
      601 taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat ttttgagcta
      661 tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataactttaa attacggatc
      721 atgatttggc atggggaatc attactaata aaataaaaaa atgaacagaa agaaaacagt
      781 tctttctgtt caccaacttc atttgacaaa tatcctattt agcttatttt atagcttttt
      841 atatacactt ttttaaaaag tgatttttcc acatgaattc ccatgagcaa ttcgaccatt
      901 atctattatt aaatcattga actgattttt ctccggaaaa acaactataa cagaagtatc
      961 tttaccagat tttattattt tgtcaaaatc cacatcaacc aacgaggttt caggagtaac
     1021 ctcatcattt tcttgaacac aaatcgaaaa aggctctcct tgtaattcaa ctgtatcaat
     1081 tccaatatga atcaaataat caatgcctga gttgcttttt atattaatag catgtttagt
     1141 aggaaatata tttgttactc gaccagccaa tggagcataa atttttgaac catcaggtat
     1201 gacggcaaaa ccatcaccta gtaatttttg tgaaaagaca ggatcattta cttctgtgat
     1261 ggaaatgatt tttccggaac aaggtgcata aaaagtttct tgttttttct ttttgaaaat
     1321 ttcaaacata ttaaaaacct cctaattatc ttttcctaat atttgattga tttcttgact
     1381 taataagtca gctttccctc caaaaacagc ttggatgcca cccttcactt cgaagacagc
     1441 agttgcacca gtgtctttta ttgtttgttt ttgaataatc tccccattcg taacagcaac
     1501 acgcaaacga gttgcacaag cttcaacaga ctcaatattt tcttttccac caagagcttc
     1561 aataatgata agagactctt catgtaatga tgtatttttg tttattactt tttcagaatg
     1621 aacttctgat tcttcttcca ttccaggaat agctacctta aattttttga taaagaatgt
     1681 aaaaacaaag aaatatataa atgcccatgc aataccaaaa ggaattaatt ttaaccaatt
     1741 agttttgtca ttcccttgaa gtacaccgaa taataaaaag tcgatcaaac cacctgaaaa
     1801 ggaattccct acacgaatat ttaagatatc agcaaaataa aaagataatc catctaagaa
     1861 tgcatgaatc acataaagcc aaggagcaac aaataaaaac atatattcaa ctggttcagt
     1921 aattccagtt aaaaatgatg taagggcccc gctaaaataa agcccaccat tctttttacg
     1981 atttttctta ggaattgcac gatacat
//
LOCUS       DACCWK010000189         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCWK010000189 DACCWK010000000
VERSION     DACCWK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.196"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..378
                     /locus_tag="IVW65_002665"
     CDS             <1..378
                     /locus_tag="IVW65_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP7901504.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IVW65_002666"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IVW65_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7901505.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IVW65_002667"
     CDS             1859..>2003
                     /locus_tag="IVW65_002667"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7901506.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACCWK010000190         1993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCWK010000190 DACCWK010000000
VERSION     DACCWK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.197"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1398
                     /locus_tag="IVW65_002668"
     CDS             <1..1398
                     /locus_tag="IVW65_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7901507.1"
                     /translation="QVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDF
                     TEESWNNFQTALSNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTIN
                     HIDNQTGQVIATDQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNT
                     TITFNYNQVHQINLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRV
                     EPTNSVTLNNVTESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWK
                     NMTDQNYFLCRNDDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELA
                     WLNDYRQQNGVAPMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAK
                     GENISQTGLYIDDLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGR
                     IGVDNVFISANPIFK"
     gene            complement(1436..>1993)
                     /locus_tag="IVW65_002669"
     CDS             complement(1436..>1993)
                     /locus_tag="IVW65_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7901508.1"
                     /translation="DGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQF
                     SIQDPVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLD
                     GREIQLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNH
                     LVLTVIGSILFLTLASLGYVVLKLK"
BASE COUNT          685 a          363 c          336 g          609 t
ORIGIN      
        1 caagtggata gtgccaaaaa taaattagat tctgcttaca aaggattaaa gaaaaaacca
       61 gttcctactg tgaataaagc tgaattacaa gctttataca accgagtaaa atcaactgct
      121 aaaggagatt ttacagaaga atcttggaat aattttcaga ctgctttatc caatgctaaa
      181 aaagtactag acgattctaa ggtttctcaa acacaagtaa acaatgctaa aaatagtctt
      241 gatgctgcat ttaaagggtt gcaacacaaa cctgaaacac caaaagaata tacggttact
      301 attaaccata ttgacaatca aacaggtcaa gtgattgcta cagatcaagc aaaagtaact
      361 tctggttcta cttacactgc taaggcaaaa gcatttaaat atagcgaagg cgctaaagat
      421 aattttagct acaaagtgat tggtaatgat actcaaagca aaacaatcac aggtaataca
      481 acaatcacat tcaattataa tcaagtgcat caaatcaatt tgttgtgcaa taacaactac
      541 aaaaacgaag gacgagttaa tattttgaaa gaacagatag tcaatgtcac tcacggtgac
      601 agtgtcactt taactgctcc ttctattcaa ggatatgttt tagacgatcg ggtagaacca
      661 acaaattcag taaccttaaa caatgtgact gaaagtcaaa attatatttt caactacact
      721 cgtaaattta atgtgactgt taatcatgta aatgctgata ccaacgctgt tctctctagt
      781 gaatcaaaaa cagtatacga aggggatagc tttgctactc cttggaaaaa catgactgat
      841 caaaattatt tcttgtgcag aaatgacgat cctaccgtta ccgtggataa aaacggccaa
      901 cgatccatta ataacgtcga tcaaaataaa acaatcacat tcaaatataa aaagatttca
      961 ttagacgaat tacgtaactt tatgcgtcaa aaagaacttg cttggttaaa tgattatcgc
     1021 caacaaaatg gtgttgcccc aatgcaattc aatgatattg ttcaacaggc tgcagatatt
     1081 cgagctaaag aattacaagt cagcttcagt cactatcgcc ctggtggtgg tactttccaa
     1141 gatttattgg aaagcttagg ctgctacggt gctaagggag aaaacataag tcaaactggg
     1201 ctgtatattg atgacctttt atcagacgat ggtgctatcg atgccatggt aggatggaaa
     1261 aatagccctg cccataatgc aaatttactt tataataacc aaagtattct cggattaggt
     1321 cataattttg tagttgactc tgctggtaga attggcgtag ataatgtatt tattagtgcg
     1381 aatccaatct tcaaataaaa gaaaacaagg ctgattctca gccttgtttt tatatttatt
     1441 tgagttttaa aactacatag cccaatgatg ctaaagtaag aaataatata cttccaataa
     1501 cagttagcac taaatgattc gatccagttt ttggcacaat gatctgaggt aagttaggtt
     1561 ttaccccaat cgttactaca ttagttgctt tctcaatact ttctactgaa acttttgctt
     1621 ggttgtcgat atccaattct tcattcaaag gagttttagc tgtaaatatc agctgaattt
     1681 ctcgtccatc taaaagattt ttacttaaga aatcagcgga agcttcccac ttcagcgttc
     1741 ttgaattgct atcataggtt agtgtacctt ctgaagtaat atcttttcct gtactcatat
     1801 ctatcactcg cgcagaacct tctttatacg tcaaacgatc ttccactgga tcttgaatac
     1861 taaattgctt gtaatgatct gaggcaatgt ctacaccttt tgtatgccat ttttgtgtga
     1921 ctagatattg tacttcagat cctgcattta aaggagctgt aggcgttcct tttttaccat
     1981 catttcgacc atc
//
LOCUS       DACCWK010000191         1979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCWK010000191 DACCWK010000000
VERSION     DACCWK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.198"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(325..528)
                     /locus_tag="IVW65_002670"
     CDS             complement(325..528)
                     /locus_tag="IVW65_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901509.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="IVW65_002671"
     CDS             complement(728..1744)
                     /locus_tag="IVW65_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7901510.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DACCWK010000192         1962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCWK010000192 DACCWK010000000
VERSION     DACCWK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.199"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IVW65_002672"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IVW65_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7901511.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IVW65_002673"
     CDS             852..1040
                     /locus_tag="IVW65_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7901512.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IVW65_002674"
     CDS             1053..1610
                     /locus_tag="IVW65_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7901513.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IVW65_002675"
     CDS             1642..1884
                     /locus_tag="IVW65_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7901514.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          305 c          404 g          537 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt ta
//
LOCUS       DACCWK010000193         1951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCWK010000193 DACCWK010000000
VERSION     DACCWK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.200"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..344)
                     /locus_tag="IVW65_002676"
     CDS             complement(<1..344)
                     /locus_tag="IVW65_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901515.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVA"
     gene            complement(341..1096)
                     /locus_tag="IVW65_002677"
     CDS             complement(341..1096)
                     /locus_tag="IVW65_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7901516.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYIMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(1109..>1951)
                     /locus_tag="IVW65_002678"
     CDS             complement(1109..>1951)
                     /locus_tag="IVW65_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7901517.1"
                     /translation="ETSPYSVFKWGDTAGQGLSYVAAKANIKVTNKDGSVVLKENTDY
                     KIQNSENGFVIDFIVNNGQVSDTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVY
                     NNNPFNQEEHHEKTKADVVTYGAKFLKVDSGLFGTGIKATPLEGAEFAVKNAEGKYYG
                     GLVDTDKDGVKEAVWVDNVANAAILKSDKEGHFEITGLTEGEYSLEETKAPENYQKLT
                     EDIAFKVNKNSFKEENRITVKNNQKAAIPLTGSNGFQTYVLISCLLLGAGGLSAVVYF
                     KKKA"
BASE COUNT          529 a          384 c          309 g          729 t
ORIGIN      
        1 tgggctactt gactgccttg attgttgatt tgattcgaag aaataatttt ttgaatatga
       61 atggcttgct tgatcacttg cgtatcttct acgtcttgcg gcttttctga agtctctgaa
      121 gaactttctg acgttggatt cgttgattca gtaaagctta tatttgaatc tgccgtttct
      181 tctgtagtat tctccgaact gtccgtttcg cttggtgcag taattgaatt atctattgtt
      241 tgagtggtac tactatcact gctgtcagcg aaagacagtt gcgaataagc tacagtagaa
      301 aagagagctg ctacacaaac aaacagccat tttctcttat tcattccgat ctcctcctta
      361 tttttttata aataaaaata ataggaacgc aaaaaaaagc gaatagcagt gctaatcgcc
      421 aatccaatga aattgatttc ctattttttg cttttttctg tagttttaaa tcttctttac
      481 tgaaggaaac tcgttcacca tacactaaaa agcggtgtga gttgacacct tttggtgtgc
      541 atgtcattaa cgtgatccta tcttggtctt tttcaggcat gatataatct aaataatttt
      601 tttgtaattc ttctgcttta acttcttcaa ttcgataaat ttgataggca tgttgttctt
      661 catttacctt aataaagaat ttttgccctt tttttactga aggcaaatca tcgaaaagat
      721 tatctttata aagtccactg tgccccgcga ttaaaggatt tttaccgttt cctcctgtaa
      781 tatcgccagt tccttccgta atccctacac cattctctaa aattttgtcg gaagttccgt
      841 cataaattgg tagttttaaa gaaatacttg gaattgtaag aacaccaaca ggatcgccta
      901 actctatttt cgcaacatcc aaagaagaat ctgttttttc aagattttca atggctaaaa
      961 ttctttgctg ttgttgaaat ttcgctacat ttgtttccac aactggaatt aaatagactg
     1021 ctactgccga cagcaaccaa aaaaatatca tcagtttgtg catgactctt ctttttgggg
     1081 gcttacgaat aatcattagc tgatccttct aggctttctt cttaaagtaa acaactgcag
     1141 aaagtccccc tgctcctagc aacaagcaag agatcaatac atacgtttgg aaaccattac
     1201 tacctgttaa tggaatagct gctttttgat tatttttaac agtgattcga ttttcctctt
     1261 taaatgagtt tttgtttact ttaaatgcaa tatcttctgt taatttttga tagttttcag
     1321 gcgcttttgt ttcttccaat gaatattcac cttcagttaa accagtgatt tcaaagtgcc
     1381 cttctttatc tgattttaga atcgctgcat ttgctacatt gtctacccat actgcttctt
     1441 tgacaccgtc tttatccgta tcaactaatc caccataata ttttccttct gcgtttttaa
     1501 cagcaaattc tgcgccttca agcggtgttg ctttgattcc tgttccaaat agtccgctat
     1561 cgacttttaa gaattttgca ccataagtca caacgtcagc tttcgttttt tcgtggtgtt
     1621 cttcctgatt aaatggattg ttgttataaa cgaaatccac gctatttgtt aatggattgg
     1681 caacagctgc gctgtcattt acatacatgt tataagtcat ttgaagatct tgtccgtgta
     1741 aacttgctac agtatcactt acttggccat tattcacgat aaaatcgatc acaaagccat
     1801 tttctgaatt ttggatttta taatctgtat tttcttttaa taccacagat ccatctttat
     1861 ttgttacttt gatatttgct ttagcagcga cataagaaag tccctgtcct gctgtatctc
     1921 cccatttaaa tacactgtaa ggagaagttt c
//
LOCUS       DACCWK010000194         1947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCWK010000194 DACCWK010000000
VERSION     DACCWK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.201"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            28..1854
                     /gene="ltrA"
                     /locus_tag="IVW65_002679"
     CDS             28..1854
                     /gene="ltrA"
                     /locus_tag="IVW65_002679"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7901518.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          719 a          355 c          403 g          470 t
ORIGIN      
        1 ctcctgcaaa caaggagggt acagaccatg ctaaagaaaa acaagctccg ctataacgag
       61 tattatgata tgcaacatat ttatgacgag ttatacgcac agagcaagaa cggtaataac
      121 ttttataagt tacttgaaat tattggttcg gaacaaaata tttgtttagc ttaccgtaat
      181 ctgaaatcaa acagtggcag taaaacggct ggaacggatg gaatgacgat agacgatata
      241 aaacaactca gcaatgctga gattgttgct accgttcggg aaagccttag caactaccgc
      301 cccaaatcag tcagacgtgt tttcattcca aaagctggga gcgataaaat gcgtccattg
      361 gggattccgt gtatctggga caggctggta caacaatgta tcctgcaagt cctagagccg
      421 atatgcgaac caaagttcca taatcattcc tatggattcc gtgcgaatcg aagtgcccac
      481 catgcagtca gcagagtaac gacactgata aatttatcaa aatatcatta ctgtgttgac
      541 gtagacatta aaggattttt cgataatgtc aatcatggaa aactattaaa acaaatctgg
      601 acacttggta ttcgggataa aagattgatt tgcattatca gcaaaatgct gaaagccgaa
      661 attgacggcg agggtgtccc agaaaaagga acgccacaag gcggtctgtt aagtccactg
      721 ctctccctca tagtccttaa tgaattagac tggtgggtaa gcagtcagtg ggaaacattc
      781 cagccgaaga accgcagtaa aaatgggtgg cttcaatacg caaagaaata taccaaactg
      841 aaaagcggtt ttattgtacg ttacgctgat gattttaaaa tcatgtgttc aacctatgga
      901 gaagcacaaa gattttacca cagtaccgta gattttctta ataaaaggct gaaattagaa
      961 atcagtccag agaaatcaaa ggtagtaaat ctgaagaaaa attcatcaga ttttctgggg
     1021 ttcaaaatca aagtcattcc aaaaggaaga acgaaacacg gttatgttgc caaaacagat
     1081 atgaaccaaa aagcattaaa gaaagcgaaa acaaatctaa agttaaaggt aaaagatata
     1141 gccagacata cgactggctt caatatcagc agatataatc tgacagttat cggtatgcag
     1201 aattactact gcatagcaac taacgtatat aacaacctga cagaagtaag ttacgctctc
     1261 ttaccgacca tacgaatccg tttaaggaac attgcaaaat cagttccatt tgaaagtaca
     1321 agttcagaat ttcagagcag gacaaaagga atcagaccaa aaacaaaaat agtgatgatt
     1381 gcggataatc cgctattgcc gattcaagga gtacaacaca aaaatcctat gaatttctcg
     1441 caagatattt gtaattttac aaagcaaggg agaaacaagg tgcatgagga cgttgtagtt
     1501 gtaacaaaag aagaaatccg agcgttgttg gaaaatgaaa accctgccga tagcgtagag
     1561 tttaacgaca atcgtatttc ggcatatatc gcacaacaag gcaactgcta tgtaatgaat
     1621 cgtcgaggaa ctccgtccac tctgatatgt attcacaaat cagacaaagg agataacctt
     1681 gacagatatt ctaatctggc atttgtggaa attcccattg caaaggcaat cctaacagaa
     1741 tcagcagacg aagcaaaatc actgttaaaa ggttatgttc ttaatagtca gcagaagaaa
     1801 aaactgaaca gaatacggac gaattacgga taccagacaa taactgggaa ataacttata
     1861 aatctatttg gatatgatgg aacgccgtat gaagcgaaag tttcatgtac ggtgtgaagc
     1921 gggggaaaac ttggagataa catcaaa
//
LOCUS       DACCWK010000195         1913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCWK010000195 DACCWK010000000
VERSION     DACCWK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.202"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            99..569
                     /locus_tag="IVW65_002680"
     CDS             99..569
                     /locus_tag="IVW65_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7901519.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            791..1894
                     /locus_tag="IVW65_002681"
     CDS             791..1894
                     /locus_tag="IVW65_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7901520.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          646 a          315 c          441 g          511 t
ORIGIN      
        1 aatctctgaa gaataaatca cgtgatcgtg acgataaaat acacttacgt caggtgataa
       61 aaaatacata agtagaaaac taagaggagg aacagaaaat gaaagaagtt caattgatca
      121 gaaaaagcga actatcagaa ggtggttgca atgcatgcgg cgtagtagaa gcaacaagct
      181 atacattaaa gcttggtgcc aacaaagcga taatctctga gttgacagta ggcggtcttg
      241 tggacagtct ggcgcttgct gaaggtttta ttggagaaga tatttatgag atgttttcag
      301 aaatccgtca gttaaaaaaa ggggaaaact gcatagaggt tcatcatgag agcccgaatg
      361 tgagattcaa acgtggtgat aatgagatga tcttcaataa tcatgtgtcc gatcacaccg
      421 agctatatgg aatcgtgaat caaatactaa ctgagctttt tggattggga ccttatgcgt
      481 tcaaggaaga aaacggtaat ccaaaattaa acgaagaatg gcaagaaacg attgaaatac
      541 aaagaaacaa cccgcactta tttcaatagg catccacagt atttcgtaaa agggactttg
      601 acacgacttt tgtcaaaatc ccatcgtttt ttatctattt gtgttctaaa catttctttc
      661 agagtagtaa caactggaat gactgaaatg taaacagatt atttgcctgt atcaatacgg
      721 aagagtaata taaagtgtgt aaacgctatc atattattca ttcaattttt tatagaggga
      781 aggcattgaa atggaaaaaa cgtatcaagc aggtacaaat gaaggaatag tggattttat
      841 caatatggaa gatttagaaa tagccgcttc acaagtgatt ccagctggtg gttatggtta
      901 catcagcagt ggtgctggcg acttatttac ttatcaagaa aatgaacggg cattcaatca
      961 tcagctgatt attcctcatg tattaagaga tgtagaatta cccgatacaa ctacacattt
     1021 tgacaaagaa acgctgactg caccaatcat catggcacca gttgctgctc atggcttagc
     1081 tcatgtgaag gcggagaaag cttctgcaaa aggtgtagca gattttggga cgatctatac
     1141 agcaagttct tacgcttctt gtacattaga agagataaga gaagcaggcg gggagaaagc
     1201 accgcaatgg tttcaatttt atatgagcaa agatgatggg atcaatttgg atattcttga
     1261 agtggccaag cgtaatggag caaaagcaat cgtattgaca gcggatgcaa cagttggcgg
     1321 aaaccgtgaa acagaccgca gaaatgggtt taccttccca ctgccaatgc cgatcgttca
     1381 ggcttatcaa tcaggagtag gccaaacgat ggatgctgtg tacaaatctt ccaaacagaa
     1441 gcttagtcca aaagatgtag aatttattgc tgctcattct gatttgccag tctatgtgaa
     1501 aggtgtccag tcagaagaag atgtctatcg ttcattagaa tctggtgctg gcggaatctg
     1561 ggtatctaat catggtggcc gtcaactaga tggtggtcca gcagcgtttg attcgttgca
     1621 atatgtggca gaagcagtag ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg
     1681 cggacagcat gttttcaaag caatcgcatc aggtgcagat cttgttgcta ttggcagacc
     1741 cgttatttat ggattgtctt taggaggaag tacaggagtc catcaagtgt ttgatttctt
     1801 taaaacagaa ttggaaatgg tcatgcaatt agctggaaca caaacagtag aagatatcaa
     1861 aaaaataaaa ttgcgtgaga atcgttttat ttaagaaaca aatgacgaaa aaa
//
LOCUS       DACCWK010000196         1875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCWK010000196 DACCWK010000000
VERSION     DACCWK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.203"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..245)
                     /locus_tag="IVW65_002682"
     CDS             complement(<1..245)
                     /locus_tag="IVW65_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7901521.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYR"
     gene            complement(359..571)
                     /locus_tag="IVW65_002683"
     CDS             complement(359..571)
                     /locus_tag="IVW65_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901522.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            758..1225
                     /locus_tag="IVW65_002684"
     CDS             758..1225
                     /locus_tag="IVW65_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901523.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            1222..1464
                     /locus_tag="IVW65_002685"
     CDS             1222..1464
                     /locus_tag="IVW65_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901524.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            1482..1784
                     /locus_tag="IVW65_002686"
     CDS             1482..1784
                     /locus_tag="IVW65_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901525.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
BASE COUNT          637 a          266 c          354 g          618 t
ORIGIN      
        1 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
       61 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
      121 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
      181 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
      241 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
      301 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
      361 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
      421 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
      481 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
      541 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
      601 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
      661 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
      721 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
      781 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
      841 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
      901 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
      961 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
     1021 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
     1081 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
     1141 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
     1201 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
     1261 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
     1321 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
     1381 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
     1441 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
     1501 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
     1561 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
     1621 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
     1681 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
     1741 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
     1801 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
     1861 ttttacgatt tcagg
//
LOCUS       DACCWK010000197         1861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCWK010000197 DACCWK010000000
VERSION     DACCWK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.204"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(378..1352)
                     /locus_tag="IVW65_002687"
     CDS             complement(378..1352)
                     /locus_tag="IVW65_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7901526.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1515..1772
                     /locus_tag="IVW65_002688"
     CDS             1515..1772
                     /locus_tag="IVW65_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901527.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          696 a          291 c          251 g          623 t
ORIGIN      
        1 cttcttagtg aattcaaata tatggtatgc ttcttagtga attcaaatat aaaagagttt
       61 aaagcgtaat acacttatgg ggaagtggtt tggggtgcgc tttaaactct tctttattat
      121 tatctcacag catgcttaaa aggtctatgg attaagttat gtaaaaaagt gcctctttaa
      181 acgtcctaaa atccaaacaa ggatagcata atatacacta tcgttgatcc agaaaacagg
      241 taaataaatt ttgcaaaagc aataagttaa aaacattaca aaaaagacaa ggatatcaca
      301 tcaagaaaaa atgttttgaa aatatctttg cctttttgta ttatatctat attaaataat
      361 caatcacaac acattaacta atttatatat ttaatttgtt gtttttctat aattggataa
      421 ctaattaact tatgactatc taagtcttct ttattcatat caattgcagt aatttgactg
      481 tcttcatatg ttcgataata atagattcct ttgtcaacat tgcaacaaga agaataaatt
      541 gtatattcat attttccatc accaacatca cacaaacctt tttgttgttc tactgaacct
      601 aagatatgga aaaattgact aatactttct gactctgaat ctccagatac agaattcagc
      661 ttcgtaaaag tcgctttaac aaaacgagat actgaggata aatctccagg caagcctatc
      721 cctcccatac cgcggctata ggcattcaaa cttatttgat ttgaaaaatt atttttagga
      781 gtttcactcg ataagacacg ataattgttt aaattaaata attgatagtc aaatgaagga
      841 ttattggtaa gaacgcccac agggttatca tatatatgaa gtccatcttt catactttca
      901 atgacaattg atttttcttt atcagctaat agccaatgta aaggggataa aggaagttcg
      961 tcactataat ttatatttgc taaattgata ttttttaaca attttttagc ttctcctact
     1021 gttgagcatt gtcctaaaat ccaaggaata aattcaaaag gagatacatt gtctttccct
     1081 tcttgtattt ctttataatc agcataccca gaaaaattta gtccagccat actcaatcct
     1141 ttttcatttg tcgcatcgta ataaagaggg tagtcagcta taccagcggc aataccaatc
     1201 attgcataat gagtatccaa atcatttacc tttcgaaaat tcaacttata atttcttgga
     1261 gtaatagtga ctacttcatt gtaagatatt tcataatcaa aattccttcc aaaataatga
     1321 tcacttgtta cataagtaat agacgtacac atagtttatt cctcctaaat atttttctgt
     1381 ccacgttcat attactccaa agttttcctc attccaaaag aaaagcacag aatacacaaa
     1441 gaagcgtttt tatttgtata aactatatca aagttctata cttaaattat caaataaaaa
     1501 ggacgtgaag ataaatgtca aatttagaaa acaaagaaga aaaagttgta aacaaaattg
     1561 tttctgttgt caataaactg gataaagagt tagacgaact tgatacgtta tctgaaaatc
     1621 cagaaaaaaa acataaccta aaaaaatggt tagttgaacg aaaggctatt catgaaatta
     1681 aaaaagttct tcatgaagct gataaatatg aaaaatatga tgaaaaagaa cttgataaag
     1741 aatttaaaga aatcaatgac ttattactat aattgattta tacgactcat ttttgagtcg
     1801 tcagactgca aacaacactc taaatttagg agtttgtcta cagtctgaat aaattttaaa
     1861 t
//
LOCUS       DACCWK010000198         1859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCWK010000198 DACCWK010000000
VERSION     DACCWK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.205"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IVW65_002689"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IVW65_002689"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7901528.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DACCWK010000199         1857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCWK010000199 DACCWK010000000
VERSION     DACCWK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.206"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            372..1754
                     /locus_tag="IVW65_002690"
                     /pseudo
     CDS             372..1754
                     /locus_tag="IVW65_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          695 a          315 c          332 g          515 t
ORIGIN      
        1 aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct tcatacgttc
       61 tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa ataaatgagc
      121 tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt aagtttctga
      181 ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa aaaagagtat
      241 tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa agaaccagaa
      301 taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat aaacttagga
      361 ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta tttttaaaat
      421 acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt ggtgcactac
      481 ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac cgaaaaaaat
      541 caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa tatggtcaat
      601 gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg cggcagcggt
      661 gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag ttaacacaag
      721 atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg aaaaatttag
      781 ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg gttcttggaa
      841 aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag ctacttttaa
      901 aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa gcattactcg
      961 aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt ctatcaaatc
     1021 aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa gagcttcttc
     1081 aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt gagaaaccag
     1141 ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa cttctgtgcg
     1201 tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct ttctgaaaaa
     1261 gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag acgaaaaaag
     1321 tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa tcggaatttt
     1381 aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag aattctcgca
     1441 gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt tagttggact
     1501 attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca ccagataatt
     1561 cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa cgtgactgga
     1621 agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat tggtataata
     1681 atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg aagaagcaaa
     1741 gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt gtaaaaaact
     1801 attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga gcaagaa
//
LOCUS       DACCWK010000200         1833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCWK010000200 DACCWK010000000
VERSION     DACCWK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.207"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            187..384
                     /locus_tag="IVW65_002691"
     CDS             187..384
                     /locus_tag="IVW65_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901529.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            490..972
                     /locus_tag="IVW65_002692"
     CDS             490..972
                     /locus_tag="IVW65_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901530.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1030..1626)
                     /locus_tag="IVW65_002693"
     CDS             complement(1030..1626)
                     /locus_tag="IVW65_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7901531.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          567 a          276 c          366 g          624 t
ORIGIN      
        1 atgttttaat tttttgaaaa aaattcttct aaatacaaca gctgttttaa ttgtgaaaac
       61 gaaaacagta cgctataatg cctgtaaggt tgatttttaa ctgtaaataa aaatcaatga
      121 gttaaatctc caaagaaata gcagtggtaa tgtggagaaa agttccagca gtttaggagg
      181 attttgatga cggaagaaat gattaattta ggagaacaat atgcatgcaa accaattggt
      241 tttacaaaaa cggtcattgg tgaagttgta tcaaaaatga caaactgcgc cgtggtcaaa
      301 gttgcccaat gtgctgcaga ggaccaagaa ttattagatg aaaaagcaag catggttgtt
      361 gcaaaatatg atacatttga ataaagaaaa caattggacg tgcggcgtgc tgcaagtcca
      421 atttctttga gaaggtaaat cagtattctt ttcctatttt ctggagaaga aaaagcgagg
      481 cgagttgtca tggtaacatt ctttacggtt ctattgtttt tattcgttgt tgtgatgttt
      541 ttatttcttt ggctggttcg aaaagaaatt atttatcgga ctgtgcgaaa tcgatgggtc
      601 tacttggtga tacccttctt agtgtcacta gtcatttggt atacactcat cagtcatcct
      661 acagcagatg aattggcaaa agggatcttg tcagcaatga tttttattag ttttttgttg
      721 gacagtagag gaatcacaga agaaggactt gttttgaata gctttgacaa aaaaggtgtt
      781 cctttttctg agatcaataa aattgtatta tatcagccaa aaggttctaa aatcgtaaaa
      841 atgaattttt tccgaaacgg atggcgagga ccgatgctga aattttctgc atcattagaa
      901 gaattagttc catttttgtc ccaacgctta aacgatgagg cagagattga tattatgatt
      961 gatccagaat aaaaacagag gttgcgacaa gatgtttgtc acaacctctg ttttttattt
     1021 caaataaaat tatccaaata cgcggaagcg atcggcatcg ttcatgtatt ctttttcccc
     1081 agccggtgtt tctggtgaac cgaatactaa ttgcgaacgt agtttccagt ttgaagggat
     1141 attccattct tttgcaactg cttcgtcaat aactgggttg taatgttgca agtttgcgcc
     1201 caatccttta tcaactaatg ctacccaagt attagctgtt gcgattccgt ttgattgttc
     1261 tgaccaatct gggaagttgt ctgcatataa ttcaaattgt tcttgtagac cttttaccac
     1321 gtcagtatct ttgaagaata atactgtacc ataaccattt ttgaaaccag ctaatttgtt
     1381 ttgtgtattt gggaaagctt ctgctggagt caatgggcga agtgcttctt cagtgatttc
     1441 ccaaagtttt tcatgtgcat ctccgaaaag gataactgcg cgtgttgatt gagcattgaa
     1501 agcagttggg ctttctttaa ttgcttcttt gatcaaagtt gttaattctt cattagaaag
     1561 tgaaacatta cgtcctagat gatagattga acggcggttt ttcaaagtgt cagtaaaagt
     1621 tgtcataata aaatttctcc tttgatttgc gttttgcatt tttttaattg attacgaaat
     1681 gaagtatagc accttacaaa aagtaagtaa acagatatgc tcacaaaaaa taagcgaagt
     1741 tcaaaaaaca attttaataa gaaaaatcgt tatgtaagga ttttcttagt tatcgctttc
     1801 attgttgtgt tttaggaatt tttgcttatt tga
//
LOCUS       DACCWK010000201         1772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCWK010000201 DACCWK010000000
VERSION     DACCWK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.208"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            79..234
                     /locus_tag="IVW65_002694"
                     /pseudo
     CDS             79..234
                     /locus_tag="IVW65_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..1530
                     /locus_tag="IVW65_002695"
     CDS             253..1530
                     /locus_tag="IVW65_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7901532.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..>1772
                     /locus_tag="IVW65_002696"
     CDS             1540..>1772
                     /locus_tag="IVW65_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7901533.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNH"
BASE COUNT          725 a          164 c          299 g          584 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat ag
//
LOCUS       DACCWK010000202         1722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCWK010000202 DACCWK010000000
VERSION     DACCWK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.209"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..246)
                     /locus_tag="IVW65_002697"
     CDS             complement(<1..246)
                     /locus_tag="IVW65_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7901534.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQE"
     gene            484..1653
                     /locus_tag="IVW65_002698"
     CDS             484..1653
                     /locus_tag="IVW65_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7901535.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          617 a          289 c          282 g          534 t
ORIGIN      
        1 ttcttgccat acctcctgaa tttgaccatc aggagtcgta atgaaaatag tatcattttc
       61 atctactaat tctcttaagt ttaaatattg tttactagat gtaaaattcg aagctgctaa
      121 tgacgagtct tcgcttttac tataaaatcc tgttacttgt attttatgtt cctggaaata
      181 cttcccgaat gaacaaccta ccttgccggc tccaataaaa ccaacgttca taattatcac
      241 ctccataaag aagtgagact acaagtccca ttcttacgat atgagcttaa aaataaaaat
      301 taaatcaaat atacagtatg tttttttcaa atatcatcca tagttaattt atcataatat
      361 cccatatttt aaaataaaag atagcgtaaa atattttgtg taaatagaaa tttagggtag
      421 aaaaaaaagt ccattctgta aaattaaagt taccacacag aaattttata ggaggacttt
      481 ctcatgacta aatttactat agaaattatg caaacactac tcaataaagg ggatttagat
      541 gaattatttc aggatcattt agagcgagcg attaattcac ttctacaagc agagctaact
      601 gcttttttag attacgaaaa atatgatcga aatggattca attcaggaaa ttcccgtaac
      661 gggaattatt cacgtacatt taaaacagaa tatggcgagt tgaatttaac tattcctaga
      721 gatcggaatg gcgaattttc tcaacaaaca ctaccagcat ataagcgaac caatgattca
      781 ttagaaacga ccattatcca attatttcaa aaaggcatta ctatggctga aatctccaaa
      841 ttaattgaaa aaatgtatgg tcaccactat acgccacaaa caatctccaa catgagtaaa
      901 ttggtggctg aagatgtttt agcttttaaa gaaagaacgt tagaagccaa ttattccgtt
      961 atatttatgg atgcgacgca tatccctgtg aagcgacaga ctgtttcgaa ggaagcagtg
     1021 tatattacga ttggtattcg tttggatgga accaaagaag ttcttgggtt tactattgcc
     1081 ccaactgagt ctgcttatat ttggaaagaa gttcttcaag atcttagaaa acgtgggtta
     1141 gaagaagttt tattagtagt gacagacgga ttaagctgta ttgaagaaag tatccatagt
     1201 gtgtatccga atgcccaatt tcaacaatgt tgtgtgcatg tatctagaaa tatcgctcat
     1261 aaagttcgtg ttcgaggtcg aaaagaaatt tgtgaggatt tcaaattggt ttaccaagcg
     1321 aattcaaaag aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag
     1381 tatccaagag tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct
     1441 cacgccatca gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta
     1501 aaacgatata ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc
     1561 atttctcaat ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa
     1621 gaaattcagg atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat
     1681 ttacatataa ttcttgacgc taccataatc taaataacca tc
//
LOCUS       DACCWK010000203         1665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCWK010000203 DACCWK010000000
VERSION     DACCWK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.210"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            117..1070
                     /locus_tag="IVW65_002699"
                     /pseudo
     CDS             117..1070
                     /locus_tag="IVW65_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            complement(1108..>1665)
                     /locus_tag="IVW65_002700"
     CDS             complement(1108..>1665)
                     /locus_tag="IVW65_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7901536.1"
                     /translation="NGRNKGDNGPATEPLNAGSEVQYLVTQKWHTKGVDIALDHYKQF
                     SIQDPIEDRMTYQEGSAQVIDQSTGKDITSEGVLTYDSHSRTLKWEASSDFLNNNFLD
                     GREVQLIFTARTPLNKQVDIDNQANVSVENIEKETNVVTIGVEPNLPQVIVPKTGSNH
                     LVLTVIGSILFLTLASLGYVVLKLK"
BASE COUNT          545 a          304 c          275 g          541 t
ORIGIN      
        1 aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc tcgtagataa
       61 gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa caccataatg
      121 atactcaaag caaaacaatc acaggtaata caacgatcac attcaattat aatcaagtgc
      181 atcaaatcaa tttgttgtgc aataacaact acaaaaatga aggacgagtt aatattttga
      241 aagaacagat agtcaatgtc actcacggtg acagtgtcac tttaactgct ccttctattc
      301 aaggatatgt tttagacgat cgggtagaac caacaaattc agtaacctta aacaatgtga
      361 ctgaaagtca aaattatatt ttcaactaca ctcgtaagtt taatgtgact gttaatcatg
      421 taaatgctga taccaacgct gttctctcta gtgaatcaaa aacagtatac gaaggggata
      481 gcttctctac tccttggaaa aatatgactg atcaaaatta tttcttgtgc agaaatgacg
      541 atcctaccgt taccgtggat aaaaacggcc aacgatccat taataacgtc gatcaaaata
      601 aaacaatcac attcaaatat aaaaagattt cattaaacga attacgtaac tttatgcgtc
      661 aaaaagaact tgcttggtta aatgattatc gccaacaaaa tggtgttgcc ccaatgcaat
      721 tcaatgatat tgttcaacag gctgcagata ttcgagctaa agaattacaa gtcagcttca
      781 gtcactatcg ccctggtggt ggtactttcc aagatttatt ggaaagctta ggctgttatg
      841 gtgctaaggg agaaaacata agccaaactg gtggttatat tgataacctt ttatcagacg
      901 atggtgctat cgatgccatg ttaggatgga aaaatagtcc tgcccataat gcaaacttgc
      961 tttacaataa ccaaagtatt ctcggattag gtcataattt taaaatttat tctgatggtt
     1021 cattaggcat agataatgta tttattagcg cgaatccaat tttcaaataa aagaaaacaa
     1081 ggctgatttt cagccttgtt tttgtattta tttaagtttt aaaactacat agcccaatga
     1141 tgctaaagta agaaataata tacttccaat aacagttagt actaaatgat tcgatccagt
     1201 ttttggcaca atgacctgag gtaagttagg ttctacgcca atcgttacta cattagtttc
     1261 tttctcaata ttttctaccg aaacattggc ttggttatcg atatcaactt gtttattcaa
     1321 aggagttcta gctgtaaata tcagttgaac ctctcgcccg tctaaaaaat tgttatttaa
     1381 gaaatcagag gaagcttccc acttcagcgt tcttgaatgg ctatcatagg ttaatacgcc
     1441 ttcagaagta atatcttttc ctgtactttg atctatcact tgcgcagaac cttcttgata
     1501 cgtcatacga tcttcaattg gatcttgaat actaaattgc ttgtaatgat cgagcgcaat
     1561 gtctacgcct tttgtatgcc atttttgtgt gactagatat tgtacttcag atcctgcatt
     1621 taatggttca gttgctggcc cattgtcccc cttattacgt ccgtt
//
LOCUS       DACCWK010000204         1608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCWK010000204 DACCWK010000000
VERSION     DACCWK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.211"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IVW65_002701"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IVW65_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP7901537.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            750..1608
                     /locus_tag="IVW65_002702"
                     /pseudo
     CDS             750..1608
                     /locus_tag="IVW65_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          522 a          307 c          305 g          474 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaaatatt
      841 ccattaaggc tccatcacaa gtgaagcgct ggatcaatgc ctatcaagaa tttggtgaag
      901 agggtctagt aagaaagcga caaaagaaaa aatattctgt tcaatttaag ctggatgcga
      961 tagagttata tctaacaagt gagctgtcct atcgtgaagt tgccaacaca cttaacatga
     1021 acaaccctaa tttgatcgct agttggatgc gtcaattccg tgagggtggg atcgatggac
     1081 tctcaaaaac gaaaggatgt ccacctatct tgtctaagaa aaatgaaccc aaaaataaga
     1141 aaaaatctac aactaaagca acgtctaaag aacgtgaacg catcgaggag ttggagaaac
     1201 gagtccgttc ccttcaaatt gaaaatgcct ttttaaaaga attgaggaag ctgcgaaaac
     1261 aggaagccca acaacgacga atgaagcaat cgcacgaatc atcgcaagcc tccgaggacc
     1321 gtttaaatta gttgagcttc tagaaacttt aaagttccct aaggcaatct ttatgtactg
     1381 gcaaaaacga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaagt
     1441 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
     1501 tttctctatc aataaaaaga aagttcagcg cctgatcaag aagctaggaa taaaagtaac
     1561 gacatacaca cgtaaatcac ggcgctacaa ttcttatcga ggacaagt
//
LOCUS       DACCWK010000205         1602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCWK010000205 DACCWK010000000
VERSION     DACCWK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.212"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            657..989
                     /locus_tag="IVW65_002703"
     CDS             657..989
                     /locus_tag="IVW65_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7901538.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT          554 a          224 c          328 g          496 t
ORIGIN      
        1 tgttaaaaga aataattaat ttcaaatata gaaaagggaa cgtatgtttg catactcctt
       61 ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac aagctaaaaa
      121 tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt tttgtataaa
      181 tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ttgcactttt ggatgataaa
      241 tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag gctggtaacc
      301 ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac ctttcagtgt
      361 tacggtatta cccgcaatgg atttagtata acagatattc aaaagtgata ccactttttt
      421 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcataatgt
      481 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
      541 tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc tctatttttg
      601 aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt tattttatgt
      661 catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta
      721 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
      781 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt gggaaacaaa
      841 agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca atcaaacatg
      901 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
      961 caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg tattttatcg
     1021 tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt cactagaaaa
     1081 ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg taggttttaa
     1141 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa
     1201 atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa gttagacaag
     1261 ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact ttgacgcaaa
     1321 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
     1381 gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat cgagcattta
     1441 ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat tttttatgta
     1501 tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt gaaaaaatta
     1561 atataaagca tagagaaaat gaaatttacg taatggagga at
//
LOCUS       DACCWK010000206         1576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCWK010000206 DACCWK010000000
VERSION     DACCWK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.213"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(837..1094)
                     /locus_tag="IVW65_002704"
     CDS             complement(837..1094)
                     /locus_tag="IVW65_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7901539.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1087..1356)
                     /locus_tag="IVW65_002705"
     CDS             complement(1087..1356)
                     /locus_tag="IVW65_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7901540.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          518 a          277 c          171 g          610 t
ORIGIN      
        1 aattaactaa ttgttgagct tcagaagtta tatctctaat tatatagtta acggaattag
       61 gaagtgtctt taactctaat gaaatttcta tccaattact atcttctaat ctgtatatgc
      121 tcgaagtata atttgattcg tcagcaagca tcgctacatc ccaaacactg atcataaaat
      181 cttttaaacg ttcaatcatt tcagcaatat tgctaaaatc agctaatttt tctttagaat
      241 aaaaaaaaca tttatatcag aatcaataaa ttctattttt tttattacta tatcgtcatc
      301 attatcttga ccatttatgt ttattgttcc ctgagtgggg agtacttctt tcaaattcaa
      361 gttaaattta tattccatta cttctcctcc aatatataaa aattacttaa ctgtatccaa
      421 atttaatatg taaataaatc attaattttt agctaaattc tatgagaagt ttatttttgt
      481 tgtttttgct aattaactaa aactagtaca aaaaatttaa aatagattat taaacctatt
      541 gcttattcta ttcagtcata tatataagcg tcatccttta gtaactaaca tcccacacta
      601 caatagtatt ataacatatc tattcaactt caaattgttg aaatttaagc catttatatt
      661 tacttttcct caactttaga aatgatttta ctaaccttat atccgtaata cgcgtaacaa
      721 acacattcat taataacaat caacactaag ctatattagc tttttaaatg actagggtaa
      781 ataaaataag gatcaaaaac ttttaagttt ctgacccttt ccttacttcc gattggttaa
      841 tagtgatctt ttgcagaata aataaatatc gtttcatttt caactctata tatcagtcta
      901 tgttcatctg taattcttct ggaccatttt ccagataaat catgctttaa tggctcaggt
      961 tttcctaatc cagcaaaggg ggaacgatcg atatctttta ttaacttgtt aatctttttt
     1021 atattgcttt tgtttccttg ctcatgccaa taaagataat catcccaagc atcatcagac
     1081 caagccttaa tcatcagatt caacctcgat taagtcatgt gttttaaatg cacctttgga
     1141 gaattgttca tctcctcgac gaattttttc catgacgtaa ttattagaaa gtgttctcaa
     1201 cgtttcttgc atagaatcat aatctctttt tgataataca acaactgtat cttctacatc
     1261 tttacttgtt acaataagtg tttcagcatc ctcattaact tgtttcatat aactacgtaa
     1321 attttggcgg aaatttgaat aagctactgc ttccattcct ttcacccctt atttatttct
     1381 ataaaacaat tgtacattat attgtacaat taagcaaatg attaatattc taacttctta
     1441 taattaaaag tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg
     1501 ctggtacctc aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa
     1561 tcctggattc actttt
//
LOCUS       DACCWK010000207         1570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCWK010000207 DACCWK010000000
VERSION     DACCWK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.214"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            54..1520
                     /locus_tag="IVW65_002706"
     CDS             54..1520
                     /locus_tag="IVW65_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7901541.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          434 a          291 c          331 g          514 t
ORIGIN      
        1 tcggtaaaga tgtataatat tttctgaaaa tttaaagaat tggggtatag attatggaaa
       61 aacagcaaga caagggcttc ttcgggcagc cgaagggatt gtcaactttg ttcttcactg
      121 agatgtggga acgatttagt tattacggta tgcgtgcatt acttatctat tatatgtacg
      181 atacagttgc caacggggga cttggattac cacgagcaac agcattagct atcatgtcga
      241 tctacggatc tttgatctac atgtctagta tcgtcggcgg atgggtatct gaccgagtgt
      301 taggagcaaa acgcactgta ttcttcggtg gagttttcat tatgcttggc cacatcgtct
      361 tggctttacc atttggtgta agtacattgt ttgtttcgat ggcattgatt attatcggaa
      421 ccgccttttt gaaaccaaat gtttctggca tggtcggaca tctttattca aagactgact
      481 tgcgaagaga tgcaggattt tcgatttttt acatgggtgt caacttaggg gcattattag
      541 caccactaat cgttggtaca atcggtcaag aatataacta tcatttaggg ttctctttag
      601 ctgctattgg gatgttcttt ggattattac aatacatcat ccaaggacgc aagacattag
      661 acggagtggg tacaacaccg ccgaatcctt tgacaactga agaacgtaaa ggattcatcc
      721 gtaacttgat catcgcactt gttgttatcg ctgcagtatt cggcggggca caactaactg
      781 gtcatctgac aattgacttt tttgtcaata caatcagtgt attggggatt atcttaccgt
      841 tgtattactt tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag
      901 tatgggctta tatcccatta ttccttgcag caatcatttt ctggtcatta caagaacaag
      961 gttcatctat cttagcttta tttgctagtg aacgtacaca atctacgcta tttggtgtaa
     1021 cgatccaagc aagctggtat caatcattga atccaatttt catctttatt ctgacaccag
     1081 tattcgtaac gatgtggacg aaacttggtg aacgtcaacc atctacagta gtgaaattct
     1141 ctcttggact tttatttgca ggattatcgt acttgctatt gatgttgccg ggtatgcttt
     1201 atggtacaag tggaaaagta agtccattgt ggttgttggg aagtttcttc atcgtggtta
     1261 ttgctgaatt gtgtctttca ccagttggtt tgtctgtgac aacaaaatta gcaccacgag
     1321 catttgaatc acaaacaatc gctatttggt tcttagctga tgcttcttca caagcaatca
     1381 atgcacaaat tgcacgcttc tacacaccgg gaacagaatc tgcttacttc ggtattatcg
     1441 gtgcagtagc aatcgtcggt ggtatcttgc tatttactgt gaaagaacca atcaaaaaac
     1501 taatgggctc tattcattaa tcaatgaaca taaaaacagg aattgcttat ttgcagcagt
     1561 tcctgttttt
//
LOCUS       DACCWK010000208         1553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCWK010000208 DACCWK010000000
VERSION     DACCWK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.215"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            220..1389
                     /locus_tag="IVW65_002707"
     CDS             220..1389
                     /locus_tag="IVW65_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7901542.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          572 a          222 c          249 g          510 t
ORIGIN      
        1 ttttatagca aagaaagttt aataagagtt tggacctagc aaacaatatt aatgaggtca
       61 ttaaatctta ttatctttag tgatataacg aaatatctac acagacaaaa tgatagcgta
      121 aaatattttg tgtaaataga tatttagggt agaaaaaaag aaagtccatt ctgtaaaatt
      181 aaagttacca caaccgaaat tttataggag gactttctca tgcccaattt tactacagaa
      241 attatggaaa cactaatcaa taaaggtgat ttagatgaat tatttcgatg tcatttagaa
      301 ttagctgtta attcattatt gcaggctgaa ttaacagctt ttcttgatta tgaaaaatat
      361 gatcgtgctg ggttcaattc aggtaattcc cgtaatggga attattcacg ttcattcaga
      421 acagagtatg gagaattaaa tttggtaatt cctagagata gaaatggtaa gttttctcaa
      481 caaactttac ctgcttataa aagaaccaat gactctttag aaaccactat tattcagcta
      541 tttcaaaaag gaattactat gtctgaaatc tctgaattga ttgaaaaaat gtacgggcat
      601 cactatacgc cacaaaccat ttcaaatatg actaaaatag tttctgaaga tattattgcc
      661 tttaaagaaa ggtccttaga atccagatac tctgtcattt ttatggatgc tactcacatt
      721 cctttaaaaa gacaaactgt ttcaaaagaa gcagtttata ttgtaattgg cattcgatta
      781 gatggaacca aagaggttct tgggtttagt cttgctccaa ctgaatctgc ttatgtttgg
      841 aaagagattc ttcaagacct aaaagaccgt ggtttaaaag aggttttatt agtcgtaact
      901 gatggtttaa gtggcattaa cgatagtatt catagtgttt atccaaatgc tcaatttcaa
      961 caatgttgtg tccatatatc tagaaatatc gctcataagg ttcgtgttag tgatcgacaa
     1021 gaaatctgta gcgatttcaa attagtttat caagcaagtt caaaagaaga agcaaataat
     1081 caaataaggt ttatgataga taaatggaaa aagcagtatc cacgagtggt taaattgctc
     1141 atgaatcctg ctatattaac cttttataat ttccctccgt caatcagaag aactatttat
     1201 tcaactaatt tgattgaagg ttttaacaaa caattaaaga aatataccaa aagaaaagag
     1261 caatttccta atgaagaatc tctggagaga tttctagtat ctcaattcaa caactataat
     1321 caaaagtttc tatgccgagt tcataaaggt tttaaagaaa tacatgatac attagaatca
     1381 atgttttaac ttgatcaaca gaatgggctt ttccatttac acaaaattat tgacgctccc
     1441 gacaaaatta tgatcttgcg taaagttata aaatattaat aaaaatattt tattaatgaa
     1501 aagcttggta gcacggttat tcatatgcta atttatttca ctttatagta agg
//
LOCUS       DACCWK010000209         1536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCWK010000209 DACCWK010000000
VERSION     DACCWK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.216"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>1536)
                     /locus_tag="IVW65_002708"
     rRNA            complement(<1..>1536)
                     /locus_tag="IVW65_002708"
                     /product="23S ribosomal RNA"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DACCWK010000210         1521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCWK010000210 DACCWK010000000
VERSION     DACCWK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.217"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..300
                     /locus_tag="IVW65_002709"
     CDS             <1..300
                     /locus_tag="IVW65_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7901543.1"
                     /translation="KGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSNDGVYRYKVT
                     RKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVVQGELQ"
     gene            353..>1521
                     /locus_tag="IVW65_002710"
     CDS             353..>1521
                     /locus_tag="IVW65_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7901544.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWG"
BASE COUNT          545 a          238 c          343 g          395 t
ORIGIN      
        1 aaaggaaact atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat
       61 attcaaaagt tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt
      121 tacaaggtaa cgcgtaaatt tatcgtacct gagtacttta aattaatcga tggcgttcct
      181 gaagagaact ctttcttgtc tttaccaaag aaaggagaga aacccttatt aacgttgttt
      241 acttgtgttt atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa
      301 caagaattta atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat
      361 aaatcggtat gtaaaaaaag ctggaatgat tgttggaatg ttaggaatca ttgcaggctg
      421 ctttcagggg tatcgagctt atgcagaaga tgtcacgcaa aaaacgccac cagaaagggt
      481 caatatcacg gttcataaat taatgtacga tcaaagtacg caattgaacg ttgatattga
      541 cggaatcaaa aatgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa
      601 caaggcagat tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc
      661 gactgaagat tctgatctaa ccaacgcgaa agtggacgaa atcgttaaag atgtagaaga
      721 caagggatct aacagtgagt acgttaagaa tgcaaccaat aaaattactt cagctgtaga
      781 tgaaaatggt gagatcactt ttgctgatca accagcctat gtcaattcca aagggaatgt
      841 atatgtggtt tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat
      901 ggttgtgatt gcgccaatga cagacaacac aggatcagga tttttaaaag atattcattt
      961 gtatccaaag aacattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg
     1021 cacagctcaa agcaaacaaa cacctttaaa aggggcaaaa tttgagttat acaagggcga
     1081 acctggaaaa ggaacaaaat taggagagct tgtatcggat gatcaaggaa agctgactgc
     1141 aactgatttg acgttaggaa aatactactt tgtggaagtt ccttcagaag ttgtagttgg
     1201 atcagatcaa gaaccaacag cagaccaata tttgttaggc gcagacgctc gcaacgatgc
     1261 gcacaataag ttaacctttg aaattacgaa tgacggcgtg acaagtgatt taaaagcttc
     1321 ctacgtgaac tataaagcac cagtgatcga taaaacagtg acaaatggaa caggagaaga
     1381 acattctttc caaattggtg acgcagtgaa ttatcaaggt acaattcata tccctacaga
     1441 tatcgcaggt ggaacagatg ggattacagt aaatggtgta aaaagtgaaa cttctcctta
     1501 cagtgtattt aaatggggcg a
//
LOCUS       DACCWK010000211         1469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCWK010000211 DACCWK010000000
VERSION     DACCWK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.218"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            62..676
                     /locus_tag="IVW65_002711"
     CDS             62..676
                     /locus_tag="IVW65_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901545.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(841..>1469)
                     /locus_tag="IVW65_002712"
     CDS             complement(841..>1469)
                     /locus_tag="IVW65_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901546.1"
                     /translation="YMHGHESIPFSLILLKRSKNISNTRDRFISIITACEIGVKEFYQ
                     QEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNKLVHSPQG
                     SKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQITLSKELE
                     RKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          482 a          254 c          237 g          496 t
ORIGIN      
        1 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
       61 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
      121 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
      181 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
      241 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
      301 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
      361 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
      421 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
      481 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
      541 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
      601 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
      661 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
      721 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
      781 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
      841 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
      901 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
      961 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     1021 atttggttcc gttggagtca attctataga ttcttcataa aaatctctat acataaagct
     1081 atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca
     1141 ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat
     1201 gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa
     1261 catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa
     1321 atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga
     1381 aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa
     1441 ggggatactt tcgtgtccat gcatgtaat
//
LOCUS       DACCWK010000212         1454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCWK010000212 DACCWK010000000
VERSION     DACCWK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.219"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            69..479
                     /locus_tag="IVW65_002713"
     CDS             69..479
                     /locus_tag="IVW65_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7901547.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            493..849
                     /locus_tag="IVW65_002714"
     CDS             493..849
                     /locus_tag="IVW65_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7901548.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          537 a          211 c          271 g          435 t
ORIGIN      
        1 tagctaataa ttggtatttt aaaagaaatt actgtagaat tggattataa ttaaaaagga
       61 gtgagaaaat gataaaagta tatacttcgc ctagctgtac ttcctgtaga aaagcaagag
      121 catggttgca agcgaatcaa cttgaatttg aagagaagaa tatttttgcc gaaccgttaa
      181 cagaaagtga gatcaaaaag atcttggcag cgactgaagg aggagttcaa gatattattt
      241 ccactcggtc gaaaatttac gaaaagctgg gcatagattt taacgaatta actttaaagc
      301 aaatgatcgt cttattcaaa gaatatcctt cattattgag acgacctatt ttgattgatg
      361 aaaaaagaat actaatcgga tttaatgaag atgagatccg gtcatttgtc cctcgtgaaa
      421 ttcgatgtgt tgccagaaat ctgatctatt tacaaaacaa taacatctta aattcatagg
      481 gagggagcct tcgtggaagt gaaagtctat ggatcagcgc actgtgtaac aagtctgaag
      541 gcgaaacaat ggttagaaca ccatcaattg ccttatcaat ttgttgactt agaagaacgc
      601 gatctaagta cagaagaagc agaagaatta tgcagttttg aagaagtgca agcagaacag
      661 ctatttgcga cttggtctga ggcttttcac aacattgcga ttgacctttc ctgtgtagga
      721 aaaaaacaac tgattttttt atgcacacaa cataccaatc tcctcagacg tcctttgatt
      781 attatcgatg aagttctatt tattggatac aatgagcagt tgatggaaca gcttatctta
      841 aataaataga ctttaactgt tttaatcaag tcactgagca tttcatacag ttgtaacagt
      901 cgaacactta gcatcaagcg tgttatgtgt tctttttttg actttatttt tctactatag
      961 agacatgctc aatttatgtg gtcaaaattc aaatctaggt ggggatatag agttaaaaat
     1021 atattgacat gtgtaaattt acatgatata ttcatttctg ttgttttaag tagcaaagca
     1081 ataaaatagt attttaaaaa acatataaaa tagttgttga catgttagaa atgacatgat
     1141 aagataactg agttgttaaa aaagaaccac ttgtgaagct tgattctatc gaaaaaaact
     1201 tcaaaaaagt tcttgacaaa taacaaatga taagttatga taaatgagtt gctgtaagat
     1261 ttcgaaaaac gaaatcaaaa aaacttttaa aaaagagttg acaattactt gtctctctga
     1321 tatgatataa aagttgctac ggcagccaaa aacgaatcgg ggaacactcc tgatgaagta
     1381 gacctttgaa aactgaacaa agtaagacaa accaaatgtg tagggcgtct tgattcaatt
     1441 caagacaaca aaca
//
LOCUS       DACCWK010000213         1432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCWK010000213 DACCWK010000000
VERSION     DACCWK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.220"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            197..460
                     /locus_tag="IVW65_002715"
     CDS             197..460
                     /locus_tag="IVW65_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7901549.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            460..732
                     /locus_tag="IVW65_002716"
     CDS             460..732
                     /locus_tag="IVW65_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7901550.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            809..1138
                     /locus_tag="IVW65_002717"
     CDS             809..1138
                     /locus_tag="IVW65_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7901551.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1128..1415
                     /locus_tag="IVW65_002718"
     CDS             1128..1415
                     /locus_tag="IVW65_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7901552.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          535 a          175 c          283 g          439 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      241 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      301 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      361 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      421 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      481 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      541 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      601 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      661 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gacgcattac
      721 gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat
      781 tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc
      841 aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa
      901 gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt
      961 tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg agattcttgt
     1021 agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac
     1081 acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa
     1141 tcgttccttc aaaacatgtc attaagtatt taaaaaagct aaaagagaag ccattgaaag
     1201 aaaaattttt gaatttaatt tatgatgaaa ttgcgattga tccatataag ggtgaacaaa
     1261 aaactgggga tttatcaggt atttggtcta gtgggttcaa atatgcgggc acaacatata
     1321 gagtagctta cgagattata gaaaacaagg ttattccagt attgttatgt ggaacgcatg
     1381 aaaactttta tgaacagttg aaaaaattaa tctagtgatt tgttgtcgtt ct
//
LOCUS       DACCWK010000214         1427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCWK010000214 DACCWK010000000
VERSION     DACCWK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.221"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1427
                     /locus_tag="IVW65_002719"
     CDS             <1..>1427
                     /locus_tag="IVW65_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7901553.1"
                     /translation="LADGQKGDNRLKTLKEIQQIYPQIPESKGGFPGYGGIPITHYKK
                     YWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAEKQSSMVVNDPKTELYVASKDE
                     LEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLTNTFTNTIYYDANAS
                     DNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYQDPDNEFK
                     QLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQ
                     STIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGKKFHEERVKVGYRGFCEINFDCH
                     LEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGNIVYRKKRGETHLPELEKEAVLTL
                     DEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTKG
                     DKCHRRIHFILDEFGNMPPMDNMEG"
BASE COUNT          514 a          222 c          282 g          409 t
ORIGIN      
        1 ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt taaaggaaat ccaacaaatt
       61 tatccgcaaa taccagaatc caagggaggt tttcctggtt atggcggaat accaattact
      121 cattataaaa agtactggta tattcagacg gatacagtaa atacttgtat tgttggtact
      181 tctcgatcag ggaaaggtca gatagaagtc ttagctacca ttgataattt atcacgtgca
      241 gaaaaacaaa gctcaatggt ggttaatgat ccaaaaacag aattatatgt tgctagtaaa
      301 gatgaactag aagctagagg gtatgatgtg tatgcgttta atatcactga tccgttgcag
      361 tcaatgagtg ataatccatt ggcactcatt gtgaaatact ggaagcgagg agacatagac
      421 acagcaacac agttaaccaa tacttttacg aatacgattt attatgatgc aaatgcttca
      481 gacaataaat actttaacga aaatgcgcaa aaagctgtca acgcgcttat ttttgcgtta
      541 ttggaacaag cggatcaaac aggggattac tccaaattaa caccaaataa tcttgttgag
      601 ttgctttctg agattggtgg ttttaattat caagatccag acaatgaatt caaacagctc
      661 aatgctttag atgaatttat gaatcagtta ccccctggaa atatagcaaa aaaacaatat
      721 ggggcaacaa aaattggatc tgataaagcg aaagggaata tcttgtctac ggcaattaca
      781 ggattgaatc catttactct aacgaaaatt gcgaagatga cttctcaatc gaccattgat
      841 tacaagtcca ttggttttcc gaagtatttg gatctcaaat tacatgaaag cctattaaat
      901 caacgcattg tcgtggaatt ttatcataag ggaaagaaat ttcacgagga acgagtcaaa
      961 gttggctatc gtggtttttg tgaaatcaac tttgattgtc atttagagca gggagatcat
     1021 gtttgcttaa aatacaatca tcaaggaaaa caatatgttg cttggtatga gttttctcaa
     1081 aaagtattga aagacgaaga gggaaatatc gtttatcgaa aaaaacgagg agaaactcat
     1141 ttgccagaac tagaaaaaga agccgttcta acattagatg agaagaaaag taacttatta
     1201 gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat gttaacgcct
     1261 gattatgatc catctaacca tatcattgtt agcatctttc ttagtcagtt gtataaagaa
     1321 ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgtaggat tcattttatt
     1381 ttagatgaat tcgggaatat gccaccaatg gataatatgg aagggat
//
LOCUS       DACCWK010000215         1421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCWK010000215 DACCWK010000000
VERSION     DACCWK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.222"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(174..1315)
                     /locus_tag="IVW65_002720"
     CDS             complement(join(174..1087,1087..1315))
                     /locus_tag="IVW65_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7901554.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          364 a          276 c          298 g          483 t
ORIGIN      
        1 atttgagtca atacctcgga cacgatttgt gagaattttt tcgagattcc agcgggtgag
       61 tatctcatcg tttcgaccag cggtcaaacc aaaaccgttt gaccgctgat cgaagcgatg
      121 aagttgaaac cctgtggaaa tctcgccaaa aaaatgtaag tggtagctag gaactaagct
      181 tgcaatgcca actcctccat ctgatttggt gttaaatagt tgatactact atggattcgc
      241 ttgcgattgt aaaatccttc aatgtattga aataacgcta aataagcagc ttcaaagttt
      301 tcatacgctt ttgatggata gacttcttct tttttgagtg aggcgtgaaa cgattcgata
      361 ccggcattat cgtagggaca gcccttacga ctgaaggagt gccgaatgtt caattcattc
      421 agctttgcct cataggcttc gctagtgtac tgagaaccca aatctgagtg gatgatcaag
      481 ttctcggagg gtgtttggtt taagagcgca ttgtcaaggg tttcgataac caattctttc
      541 gtcatctgct tgccaaactt ccaaccgatg actttctttg tgtggaggtc ttgaatagtg
      601 gagaggtagg tccagccatc ttttagggta tgaatatagg taatatctgt cacccatttt
      661 tgattgatcg acgtagtggt aaaatcttgg tttaacaggt ttttacgtcc ttcactttta
      721 actttcgctt gtttgccagg cttgtacttc ttgagggtga ctgaacgcaa gcctaatttt
      781 ctcatcagta tttgtacttt ctttaaggac aaggtctcgc cacggccaac aagaatctta
      841 tgaattttag gcgcaccata gatacccttg ttttcaaagt agattcctga aatcaagcgg
      901 tttaagcgtt tgttttctga ttggcgcttg gaaggtttct ttttcttcca gtcatagtag
      961 ctggatttcg gtgcttccaa aacggtacac atttgtttta cgtcatgatc atgaacattt
     1021 gattgaataa attcgaagac atcactcatt tttacttttg agcgaatatg gttaaagcct
     1081 tttttaggat agtgttttcc tcctgcatgc gggccatttc ctttttcatc gctaggattt
     1141 ctgcctcagt catgccggtt gctacattct ttgtatgaag cttcttccaa cgataaatcg
     1201 tttgtgtagc gataccatat tgcgctgcca gttcttcgac cgattggcca gactggttta
     1261 aatctaagat catcttctta aagtcttcat caaatttttt cttggctttt gccatcgtgg
     1321 acacgtcctt ttcttagtag ttagattcta cggttctgtt gcaaagtttt aaatctacta
     1381 tcaaataaga tagaataata gaaaaagata gcaggaggaa t
//
LOCUS       DACCWK010000216         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCWK010000216 DACCWK010000000
VERSION     DACCWK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.223"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            37..1338
                     /locus_tag="IVW65_002721"
     CDS             37..1338
                     /locus_tag="IVW65_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7901555.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          446 a          275 c          253 g          398 t
ORIGIN      
        1 ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa
       61 ctactacttg gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt
      121 aagaatatca agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct
      181 ttgtgtaaac agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc
      241 attcaactta ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac
      301 cactgtcgtg gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc
      361 tttatcgcta aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg
      421 acgacaattg ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct
      481 ctgatcccca tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa
      541 tttcgttcac tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact
      601 ggaaaattac tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg
      661 caatttgaac gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct
      721 tatgcctctc tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac
      781 attgtcaaac atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt
      841 gatcgaaacg atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt
      901 ctgatcaaac gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt
      961 tgggcgtatc tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta
     1021 cgaatcgcct atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac
     1081 actttcttta aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata
     1141 aaaaaagctt ttataaacta tgaatccggc attcgtttag ccctcgaatt gccttattca
     1201 aatgccaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgagt cgcttatggc
     1261 tttcgaagct ttcggaaaat gaagaccaga attttcttac taaataatct gattacctat
     1321 gaatcaaaaa acatctaaag tagaaaagga attctacctt agatgttctt at
//
LOCUS       DACCWK010000217         1367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCWK010000217 DACCWK010000000
VERSION     DACCWK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.224"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            96..1181
                     /locus_tag="IVW65_002722"
     CDS             96..1181
                     /locus_tag="IVW65_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7901556.1"
                     /translation="MSTEIQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT          563 a          193 c          215 g          396 t
ORIGIN      
        1 tactgaaagt gttttattca cccttaatac atggtatagt tcacttgttg aaaatcgtga
       61 atatatacaa gtttctaaga agaaggagaa atttaatgtc tacagaaatt caatctcaaa
      121 aaatgattat taaaaattgg ggatataaag caacgaatgt ttatattgaa aaatattata
      181 aatttcctag ttttttaatt cagaattcta aatactctaa aatgaaagac agctcaaaaa
      241 tagcctatat gctttttaaa aatgaatttc gtagagcctt acatgaaaaa tggatcgacg
      301 atgatggtct attatatttg gaatttacac aagcagacct aatggacaaa ttaaattgct
      361 accaaggcaa agtaaaaacc attttaaacg agttagaaac atataatttg atttcagtcg
      421 taaaaggaga atttaaccca aaaacgaaaa aaaatgaaaa aaataaatac tatttacttc
      481 aacctgaaat aagtgccgat gatcttttta ttcaaaattc tttcgatgac gatttaagcg
      541 aaactattga taaatcaacc tcttctcgga atgcggaaat cgcacgtcga gaggaaactg
      601 ctaaaaccct tgataatact ggaaatgcgg aaatcgcacg tcgagaaaaa acagctgaaa
      661 cccttgataa cactggaaat gcggaaatcg cacaagatta ttatattaat aaatctttag
      721 acactaaaag acacttaaaa gacactgaaa cagaccaatt acaaaatcaa gttttgctag
      781 ataacttcgt tgaaatcatg aaagaagaca gcataaacac atttgtacca gaaaaggtat
      841 taagcttgat aaaaacattt tctaacactt acgaagaagc ccaacaaacc gtcaccacaa
      901 tacacaacgc aaaacataaa gccgaggaac ttgagaagac tgtagttgtg tatgaagaac
      961 ttgaatctta cggaattgat gcggataaag gattatatat gacgttgctg aaagcttatc
     1021 aaaaacaaaa gactgagaaa gtaaataatt tgcagaattt gatttttgtg tatgtaaaaa
     1081 actggtttat tgaaaaagct attgcggcaa agcttgcaaa cgaacaaaga gaaacattta
     1141 acgaattacc aacagttagt acggaaaatt ggctagagta gttgataaaa agcgatcact
     1201 ataaaaatga tcaattttaa attagaactt ttttatgatt ttaattttat tacaaatgtt
     1261 acaaataaat agttattatt ccggagtttt tagattaaaa gtttatgaaa acaatcacaa
     1321 acgtaggttt taaagtcata atcgttagat attttagtta tttttca
//
LOCUS       DACCWK010000218         1360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCWK010000218 DACCWK010000000
VERSION     DACCWK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.225"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            82..>1360
                     /locus_tag="IVW65_002723"
     CDS             82..>1360
                     /locus_tag="IVW65_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7901557.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          463 a          262 c          257 g          378 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat
//
LOCUS       DACCWK010000219         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCWK010000219 DACCWK010000000
VERSION     DACCWK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.226"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..731)
                     /locus_tag="IVW65_002724"
     CDS             complement(<1..731)
                     /locus_tag="IVW65_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HAP7901558.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKVITRFVFII
                     FALYSLLGIMSGDRGSWLYSSIILI"
     gene            complement(774..>1316)
                     /locus_tag="IVW65_002725"
     CDS             complement(774..>1316)
                     /locus_tag="IVW65_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7901559.1"
                     /translation="SSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIYGEGGL
                     KTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALAIGVP
                     VIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKGIKLR
                     KELTSKNISNIWYEYLNQFF"
BASE COUNT          462 a          257 c          147 g          450 t
ORIGIN      
        1 catatgagta taatagagct atacaaccaa cttcctctgt ctcctgacat aattcctaat
       61 aaagaatata aagcaaaaat aataaaaaca aatctcgtta ttacttttga aaagttgcta
      121 ccaattaata aacatattaa ggctggaaaa aaaagatata gaataatttg taaatatcca
      181 ctttgtgtag cataatcacc ataatataat gcattataac catggactaa tgctatacgt
      241 acctctagta atttagaaac aaaaactatc ggtactacta ttatagaaat gatacaccct
      301 attttaaata aaaactttct atcggcatca gcatctccag attcatacca actaactttt
      361 cttagtgtgg tttttctcgt aaataagagt gctcctatgt gaaatacaaa catagaaata
      421 caagaatacc attttaccat tatcatatct gattcactag gaataatccc cgtaccataa
      481 gctactactg ttgtaccgat tccacgatct gttccaatac cgaatgccca cataatgcat
      541 tgcccataac taaataataa aaaaagagtg aaaaaaatcg tgtataatga aaatattgaa
      601 tctccttttc tccaatgcca tgtacagatt gcatataagt aaatcaatat tccaagccaa
      661 tttagccaat agttctgatt taatgaaaaa gcgcaaaata ttgtaaacgc gactaacgtt
      721 aacgaaaaca tcaatatatt tattttttct ttcacacaat atcctccttt tggttaaaag
      781 aactggttta aatattcgta ccatatattt gaaatatttt tagatgtcag ttcttttctt
      841 agtttaattc ctttttgcgc ttttcttacc acactttctc tatccaaaag taccctacgc
      901 attgcatctg ttaacttatc tagattatta cattcaacaa gatgacctat ttcatttcta
      961 tcacctaaat acattcttgc gccacctact ggacaatctg tagcgataac tggcactcca
     1021 atagccagtg cttctaacat agaattagat ataccttcat aatcagaaga aattacaaat
     1081 cctgccgcat tttgcatctt ttcatgtatt ttatttgtaa attcttttag atgaacaaat
     1141 tctgttaatc cattatggtt gatataactt tcaagctcgg tttttaatcc cccttctcca
     1201 taaatatcca gttcacactc atatccttca tgaataagtt ttcctattgc atcaatcatt
     1261 aagggcaaat ttttttgttt atccaatcta catgcagtaa taaatttttt actact
//
LOCUS       DACCWK010000220         1300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCWK010000220 DACCWK010000000
VERSION     DACCWK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.227"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(62..280)
                     /locus_tag="IVW65_002726"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IVW65_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IVW65_002727"
     CDS             complement(546..740)
                     /locus_tag="IVW65_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901560.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          461 a          217 c          177 g          445 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt
//
LOCUS       DACCWK010000221         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCWK010000221 DACCWK010000000
VERSION     DACCWK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.228"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..148
                     /locus_tag="IVW65_002728"
     CDS             <1..148
                     /locus_tag="IVW65_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP7901561.1"
                     /translation="YFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVVQ
                     GELQ"
     gene            201..>1292
                     /locus_tag="IVW65_002729"
     CDS             201..>1292
                     /locus_tag="IVW65_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7901562.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDI"
BASE COUNT          475 a          204 c          285 g          328 t
ORIGIN      
        1 gtactttaaa ttaatcgatg gcgttcctga agagaactct ttcttgtctt tgccaaagaa
       61 aggagagaaa cccttattaa cgttgtttac ttgtgtttat acgtcacaag gaaaagaaag
      121 atacgtggta cagggagagc tacaataaca aggttttaat caacgcgagt cgttgaaata
      181 tgaaatgagg agagtaaaaa atggcaataa atcggtatgt aaaaaaagct ggaatgcttg
      241 ttggaatgtt aggaatcatt gcaggctgct ttcaggggta tcaagtacat gcagaagatg
      301 tcacgcaaaa aacaccacca gagaaggtca atatcacggt tcataaatta atgtacgatc
      361 aaggaacgca attgaatgtt gatattgacg ggatcaaaaa cgatggttat acgcacgatc
      421 aatatcctac aggtgtaacg aaatacaaca aggcagatta tggtgatgtt gagtttacgt
      481 taggaaatat tacggatcaa gtattaccga gagaagattc tgatctaacc aacgcgaaag
      541 tggacgagat tgtacaagat gtagaagaca agggatctaa cagtgagtac gtcaagaatg
      601 caaccaacaa aattacttca gctgtagatg aaaatggtga gatcactttt actgatcaac
      661 cagcctatgt caattccaaa gggaatgtat atgtggttta tgaaagtaaa tccgctgctg
      721 gattagtcac acaaaaagca aaaccaatgg ttgtgattgc gccaatgaca gataaaacag
      781 gatcaggatt tttaaaaaat attcatttgt atcctaagaa cattgtttct aagttgagtt
      841 ttgaattaac gaaatttggt gacgatggcg cagaccaaag caaacaaacc cttttaaaag
      901 gggcaaaatt tgagttatac aagggcgaac ctggaaaagg aacaaaattg ggtgatcttg
      961 tatcggatga tcaaggaaag ctgactgcga ctgatttgac gttaggaaaa tactattttg
     1021 tggaagttcc ttcggaagtt gtagtgggat cagatcaaga accaacagca gatcaatatt
     1081 tattaggcgc agacgctcgc aacgatgcgc acaataaatt aacctttgaa attacgaatg
     1141 atggcgtgac aagtgactta aaagcttcat acgtaaacta taaagcacca gtgatcgata
     1201 aaacagtaac aaatggaaca ggacaagaac attctttcca aattggtgac gcagtaaatt
     1261 atcaaggaac gattcatatt ccaacagaca tt
//
LOCUS       DACCWK010000222         1283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCWK010000222 DACCWK010000000
VERSION     DACCWK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.230"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            7..624
                     /locus_tag="IVW65_002730"
     CDS             7..624
                     /locus_tag="IVW65_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901563.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          484 a          166 c          202 g          431 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taa
//
LOCUS       DACCWK010000223         1275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCWK010000223 DACCWK010000000
VERSION     DACCWK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.231"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(31..1203)
                     /locus_tag="IVW65_002731"
     CDS             complement(31..1203)
                     /locus_tag="IVW65_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP7901564.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DACCWK010000224         1259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCWK010000224 DACCWK010000000
VERSION     DACCWK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.232"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            230..1087
                     /locus_tag="IVW65_002732"
     CDS             230..1087
                     /locus_tag="IVW65_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7901565.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          465 a          184 c          253 g          357 t
ORIGIN      
        1 gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat aacgtcatta
       61 ctttgaggaa agctataaat caatatattt cagcttttcc ttatttatgt actacctttt
      121 acttattaat tataaggtag tacatatata tctttttttg gtagtaacta aaaataaata
      181 tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg tggtagcgaa
      241 gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg cgcaaatgtt
      301 agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact tggatatgca
      361 aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg aagaaggagg
      421 cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga taaacaatct
      481 tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc cagcagacat
      541 gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat tatcccaatt
      601 caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca tctatttaga
      661 tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg actgggcaat
      721 cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc ttgagtatat
      781 ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta ttcctttcat
      841 gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag agatttatgg
      901 ggaacatttg attaaaaatg ttgtactcaa aaatgcgcgc ttaaaacgat acgacggatc
      961 aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc gcaaaagcca
     1021 cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt atgcggaggt
     1081 ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag gactcgccaa
     1141 aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat ctgaaaataa
     1201 gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg aaagacaga
//
LOCUS       DACCWK010000225         1258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCWK010000225 DACCWK010000000
VERSION     DACCWK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.233"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            140..1180
                     /locus_tag="IVW65_002733"
     CDS             140..1180
                     /locus_tag="IVW65_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7901566.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          505 a          179 c          204 g          370 t
ORIGIN      
        1 tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat gcgtttgcat
       61 tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa taaaacaacg
      121 tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata gagtttacaa
      181 cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca aaaaaatgaa
      241 agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg caatcagcaa
      301 acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg aactatgtag
      361 agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg aatcctttgg
      421 tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata atcctaatag
      481 actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg aaaaatttga
      541 tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac ccaccctcga
      601 cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca taccgtgcca
      661 aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata atatattaga
      721 cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat tgttgttaga
      781 caattttgag acaattatga caaacgacag cattgctacg tttgtccctg aacgagtatt
      841 aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg tccagactat
      901 tcataatgca aagaaaaaag ctgaaataga aagtgatatt tcgatagttt ttgaagaact
      961 cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa aagcctatca
     1021 aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct atgtaaaaaa
     1081 ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt tgaattatgc
     1141 aagctcccca agcactatta cgaaagactg gttagagtga aactttactg acaaagtgat
     1201 tgttgcttca tggagtaaca ttaaaattaa aaatttttta ttattctaat tatattac
//
LOCUS       DACCWK010000226         1256 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCWK010000226 DACCWK010000000
VERSION     DACCWK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.234"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(258..1118)
                     /locus_tag="IVW65_002734"
     CDS             complement(258..1118)
                     /locus_tag="IVW65_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7901567.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          385 a          238 c          182 g          451 t
ORIGIN      
        1 aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg ttgcttattt ccctctaact
       61 atcgttttct atttgatggt taacctactt aaaataaata ctgcaacgac aaacggagat
      121 caagcatgct ttgtaacgta ttttacgata attacgactt tattttatta ctctatcttg
      181 ctgccatacc cgtctatccc taatcatctt tggtgaattt tttcaaagtt agcataacaa
      241 aaataagtat tttttcctta gttaatcaat agtatcattt ttttcatatg atttaattca
      301 tcaattaatt ttttgttttt attaatttta gcaaaagcta gtgcatcttg aatcaattcg
      361 agcacttctg ccttcgactt atttttagcc tgtgcgttct tcgctaattg ggcagcagct
      421 cgtgctaagt aataagtcaa atggttatcc gaacaaatat cgattgcgta atttagtagt
      481 gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa caccacaatg aaagacgata
      541 tttaacaccc gccagatatc ttctgttgta cgaatcggat acgtataaat tttttcaaac
      601 actcgattga aataaacttc tgctttttcc aactcttttt ctctagcata gaccattcca
      661 atacctgtat atgccaataa acgaaacaac gtgttggctg tttctgccat caacaactga
      721 tcaaaatgga acaacacatc cgtaattggt gcattcgtaa aaatcatttc aaatcctttt
      781 agatagtagt aacgtgcaat cagctcagac tcgtcaatta ccttgggatc aatggctgct
      841 aaaatctgtg tcaattgttc atattcactc gtaatcagtg aaaactctgc tttttctagc
      901 tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa aaagttcaac taacggaaga
      961 ttgattcgat tacataattt gattaaaatt tttaacgaag ggacttgacc attgttctca
     1021 aatttactta atgttgcttg tgtacaaatt ccttcagaca cttctttttg agataagttg
     1081 gcagcctttc ttgccgtaat aaatttttca atgttcatta ttttctctcc aatcagacct
     1141 cacacgaaaa tcatttctgt ctcgatttta gcatgttttc tttgggaatg ctttaggttc
     1201 tgttgcaaag ttttaaataa agaataaagt cccttacggt atctatgatt taagct
//
LOCUS       DACCWK010000227         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCWK010000227 DACCWK010000000
VERSION     DACCWK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.235"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1138
                     /locus_tag="IVW65_002735"
     CDS             join(<1..252,252..1138)
                     /locus_tag="IVW65_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7901568.1"
                     /translation="SKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKWMKQAQSTGS
                     FKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANKHKYSISAMC
                     KILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRR
                     IGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVG
                     KKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDN
                     QTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDY
                     VHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          410 a          242 c          273 g          321 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaaa attctaaata tttctcgtca gacctactat tatcaagcga aaccgatcga
      361 aaatgagtcc gacttagaag agatcgttca ggaagagttt attcgaaacc gaaaggctta
      421 cggtacccga aaattgaaga agtgtttagc aaagcgtggg cttcaactca gtcggcgccg
      481 aatcggtcga atcatgaaac gccgcggatt gacatctacc tatacgatcg ctcattttaa
      541 agggcaacga acagcttgta atgaagcgaa aacagcgaat gtattagatc ggacctttac
      601 acaagaacag ccattggaag ccatcgttac ggatcttact tatgttcgcg tggggaaaaa
      661 gtggcattat atctgcttaa tacttgattt gtttaatcga gaaattattg gttattcctg
      721 tggtgagaag aaagatgcct cattggtaaa agaagccttt ggacggatac cgtattcttt
      781 aacagacgtc aagctttttc atacagaccg gggaaaggaa tttgataacc aaaccattca
      841 tgagattctg aatggttttg gaattactcg ttcattgagt aggaagggtt gtccgtatga
      901 taatgccgtt gtggaatcaa cctataaatc tgtcaaagta gaattcgtgc atcaatacca
      961 atttgagaca ctggcacagc tacgtctaga attgtttgat tatgtgcatt ggtggaacta
     1021 tctacgctta catggcacgt tggcgtatga aacaccgatc caaattcgac aacagagatt
     1081 ggcgaagcga atccttgata atgagcgcgg atctgatacc tctggagagg cagcgtaatt
     1141 gaatgattgt gcttctgccg gagaaaatca gatccgagga tgctcattgt caagggcaat
     1201 cgtagccata acaccgcagc attcacaaca ccttataatt tttgtc
//
LOCUS       DACCWK010000228         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCWK010000228 DACCWK010000000
VERSION     DACCWK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.236"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            200..742
                     /locus_tag="IVW65_002736"
     CDS             200..742
                     /locus_tag="IVW65_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901569.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IVW65_002737"
     CDS             832..>1246
                     /locus_tag="IVW65_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7901570.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACCWK010000229         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCWK010000229 DACCWK010000000
VERSION     DACCWK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.237"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1076
                     /locus_tag="IVW65_002738"
     CDS             <1..1076
                     /locus_tag="IVW65_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7901571.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACCWK010000230         1135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCWK010000230 DACCWK010000000
VERSION     DACCWK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.239"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(180..>1135)
                     /locus_tag="IVW65_002739"
     CDS             complement(180..>1135)
                     /locus_tag="IVW65_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7901572.1"
                     /translation="RYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYH
                     KVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPM
                     VKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEE
                     TPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGRE
                     HEERYMDEKGIVQDDYRIEFIGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFKN
                     RYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK"
BASE COUNT          331 a          241 c          174 g          389 t
ORIGIN      
        1 ttttcgaata aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
       61 aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca
      121 caacctcttt tatttcaaat aaccagtaaa tacaatgaac atctacaaaa aacttctatt
      181 tatttataga tttcgtcagg caaatccaat tctctcttat caatcaaagt ctgataccac
      241 gaagcagatt ttttcaatgt tctgcttcgt tcatcgttca attcgattcg aactaatcca
      301 tatcgattct taaatgcatt cattggtgac acacagtcag tgaatgccca taacatatag
      361 ccttcgcaat ttgcgccttc ttctgttgct ttgatcaaat aacgcaaatg ctgtccgata
      421 aattcgatac ggtaatcatc ttgcacgatt cctttctcat ccatataccg ttcttcatgt
      481 tctcttccca taccgttttc tgttatcagc caagggatat tatgataatt ttcttttaga
      541 tacatggcta tatcatacat gattttcgga tagatttccc atcccctgga attattcatt
      601 ttcttcccag gtaaatcaaa gacttcataa tatcgttctg gatggaaagg tgtctcttca
      661 ttccattgat aacgaggagc gcgaacgcgt ttaggataat attgattgat tccaaggaaa
      721 tctactgtat gatgctgaat cgtttctaaa tcctctgttg caggatcgaa ataaatcgat
      781 tgtttttgac acaactccag gaactcttct gtgtagtacc cttttaccat cggatcaaaa
      841 aatactcgat tataaaacaa atcatacata taagcagctt tttggtcttc tggagaatta
      901 gatcgagcat agaccatttc aggatttaaa atacagccaa tccttccacc ataatttccg
      961 ctgcggtaaa tctctactgc tttagccgtt gctaaaactt tatgataatt ccacatcatc
     1021 cacttttttg tgttttgttc atgcggccag cgaacagcat ccaaatagca acgcgtttgg
     1081 ggaacgattg gttcgttgaa tgtaaaccag ttttttaccc tatcaccgta acgat
//
LOCUS       DACCWK010000231         1087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCWK010000231 DACCWK010000000
VERSION     DACCWK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.241"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..863)
                     /locus_tag="IVW65_002740"
                     /note="aph(2'')-Ia"
     CDS             complement(<1..863)
                     /locus_tag="IVW65_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7901573.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRT"
BASE COUNT          358 a          182 c           83 g          464 t
ORIGIN      
        1 gtccttttat aaatttcttt tctaccattt tcgataaatt cctgtttaat atttttaatt
       61 ccataaacaa tagtttcaat aggataatat tcttcaacta tatcttgata ttcttttgct
      121 ttctcaatat ctatatttcc atacattctt aatatatctt ctccaaaatt tgttcctatt
      181 tcttcttcac tatcttcaag taagtatata aaatcacaat attcatctat aattccagaa
      241 tctccaaaat caattattcc agttaatcta ttattgccat ctaacaatag atgattacaa
      301 ctaaaatcat tatggcataa acacttttta ccctcaaaaa ctgttgttgc atttagtctt
      361 tccataaaac tttctatata atctttttct atatcagtta aatcattata aatagtttca
      421 cgcaacaata tatactcttc taatacattt tgtttattat caatagtaca ttcactaata
      481 tctgtataat ctaaaccgtg catttgtctt aaaaaactgg caatatctcg ttttaacaaa
      541 ttttgttctt cttctgacat agtagaataa atttctggtg ttaaaaaagt tcctttaatt
      601 tctttataac ctagtataga taattcatca ctaatatacg aatattcaat attaggaatt
      661 tttacattag tttctaaatt tgtatttaaa aaattatata ttgctttttc ttttgcataa
      721 ccttttttct tattagtact aaattttgtt ttaaaaatgt attcattatt aactaaatat
      781 gccacactat cataaccact accgattatt tcaatactat ctactttgaa attatcaaag
      841 taatgctcaa ttaaatattt cattgcctta acatttgtgg cattatcatc atatctatat
      901 tccattaaat aacaatcttc ttttttgccc tcgtgtaatt catgttctgg caaatcttca
      961 ataattctaa aaccagattt ttggtatgcc cttattgctc ttggattatt tttatgaggg
     1021 tctaaaataa ctgcattagc atttctttct tttttcaaaa attcaaaaat caatttaata
     1081 tatcttg
//
LOCUS       DACCWK010000232         1081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCWK010000232 DACCWK010000000
VERSION     DACCWK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.242"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..903)
                     /locus_tag="IVW65_002741"
     CDS             complement(13..903)
                     /locus_tag="IVW65_002741"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7901574.1"
                     /translation="MLSENLNSLVQFIEKWCKDYTVSLLCRLLEIPRSVYYFYKNKPL
                     TATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSI
                     VVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(885..>1081)
                     /locus_tag="IVW65_002742"
     CDS             complement(885..>1081)
                     /locus_tag="IVW65_002742"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7901575.1"
                     /translation="SVRGLAKEYGLSEATIYKWKNLYLPDQSTGLTGKEVAELRKENA
                     RLNEELEILKKAAAIFSRKT"
BASE COUNT          303 a          186 c          178 g          414 t
ORIGIN      
        1 tagtagagac ggttaagcca ttttttcttt tgctaattct tcaaattcct gtggggttag
       61 atagtgaatc gagctatgga ttcgatttcg gttataaaat ccttcaatgt agctaaatag
      121 cgctcgattt gcttcttcaa aatctgagta gctagtcgtg tagacttctt cttttttcaa
      181 tgaggcatgg aaagattcga ttccagcatt atcataaggt gttccttttc tactataaga
      241 atgccttatt ttgttggttt taagccattg ttccacttct cttgctgtgt attggctgcc
      301 caagtcagtg tgtagaatca ttccttctgg aatgtgataa tgtatttttg ctttattcaa
      361 tgtttgaatg acacaatcca cagtcattcg ttttgaaaat gtataactaa taattttttt
      421 cgtgtgtaaa tccatgattg aagataagta acaccaacca tttttcttag taggaatgta
      481 cgtaatgtca gctgcccatt tctcacagat cgtttcagta gaaaagtcct gatttaagag
      541 attctctttt gaaatgatcg gtttattaga cctttgaggt ctatattttt taactacaat
      601 cgaccttaaa tttaattgtt tcattagctt ttggacatgt ttaagagata ctgaaatccc
      661 ttcttttaat aaaacttgat ggatctttgt ggcaccatag cgttgtttat tcgtaaaaaa
      721 aattgttgaa atcttctttt tcaacttatt atttctgatt tctgtagctg tcaacggttt
      781 attcttataa aaataataga cacttctagg gatttctaat aaccgacata acaaagaaac
      841 agtgtaatcc ttacaccatt tttcaataaa ttgaacaagc gaatttaggt tttccgagag
      901 aatatggctg cggctttttt taagatttca agttcctcat ttaaacgagc attttctttt
      961 ctcagttcag ctacttcttt tccagtcagt cctgtggact gatcaggtaa atataaattc
     1021 ttccatttgt aaatcgttgc ttcagataag ccatattctt tcgctaaccc tcgaacagaa
     1081 c
//
LOCUS       DACCWK010000233         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCWK010000233 DACCWK010000000
VERSION     DACCWK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.243"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            122..862
                     /locus_tag="IVW65_002743"
     CDS             122..862
                     /locus_tag="IVW65_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901576.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          396 a          186 c          201 g          290 t
ORIGIN      
        1 actatatata tatttatgaa attcatcgct attttcttaa aataatcagc gcattgctac
       61 tgaaaacaat gaatctgtgc tattattgag aaaagttcca tttttgagaa ggatggtttg
      121 aatgaaagaa atcagacgaa aacatttccg ttttcctgcg tacgatgatg agttaggcgt
      181 aaaattaacc aacgacaaga agcgagtatt atttgacgga caagaagatc ttttgactga
      241 acagaatcag tccccgtttg aacagatgaa agaatttcat ttcacgaaca cgaacaccga
      301 gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc gtaaggaaaa
      361 tttggaaaag cataaaggaa aattaccgga ttataccggc cattaccaat cagaaatcac
      421 atcgacagga cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac aaagtgaaac
      481 aaggacagag tccaagcata ctacacgaaa cgcatctgtt gacacgaagg tgcgggagcg
      541 ttcttatttt gttcctaaat atgtacccgc atcgattatc ccagacccta gagaaagcaa
      601 aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt atctgttgtt
      661 cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg tacgaacatt
      721 caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat cgaaaaaaag
      781 ccatggtatc ttagaacgct cattacaagg attgatcgat gatcaaggaa actctttggg
      841 agaaaatagc tattttaaat aagtgatata caaatttata ttcggctaca gttggaaagg
      901 aaacgctgcg gcgtttcctt attttatact ctatccgaaa gtttatatcg tatcctcaaa
      961 tttttttgag tttaagcgaa ttttttctcc caataagtaa gaaaataatg gcaaaataaa
     1021 aaaacaaact attttcgcag atcgtttgca aaaatagttt atttttttat aaa
//
LOCUS       DACCWK010000234         1067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCWK010000234 DACCWK010000000
VERSION     DACCWK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.244"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="IVW65_002744"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="IVW65_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7901577.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DACCWK010000235         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCWK010000235 DACCWK010000000
VERSION     DACCWK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.245"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(37..993)
                     /locus_tag="IVW65_002745"
     CDS             complement(37..993)
                     /locus_tag="IVW65_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901578.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DACCWK010000236         1024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCWK010000236 DACCWK010000000
VERSION     DACCWK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.246"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..998)
                     /locus_tag="IVW65_002746"
     CDS             complement(<1..998)
                     /locus_tag="IVW65_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7901579.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNE"
BASE COUNT          358 a          163 c          117 g          386 t
ORIGIN      
        1 atttcattct taaagctttc tcttaattta gtttcactta cagaaaaata attagctatt
       61 tcctttacag taattctttt atagatattt aagccgatga attgaacgac agaagaaatt
      121 aagggagaaa gatgctctat cttagttttt ccgatttcct tagtataata aacaatccca
      181 gaatccctca cttttaagac ctcaggtaac gatacagcta attcgttttc ttgtatcaaa
      241 gcatctctac tttgatacgc ttctattata tccattccta atgttataga cgtttgtgct
      301 aacttttcaa aaacaatgat agaataattt ttttcagacc gaatcgtatc tccactaaca
      361 cttggaatta ttccattact cagattaaaa accatgtttt ctaaatttcg aatatctcca
      421 tttttaacat attccagtat tttattttca taaagaaaca gatatatttc tggatcgtaa
      481 ttttgtgata acagattatc tattttgatt tgctcgatag ttttacttag attttctgta
      541 tattcaatta acggttcatg gaatagatct tctatttttc cagtaaaaaa ataatgaact
      601 aaaatcagaa tatctcgaat gtctcctaaa gaatacaaag tcaaattttg caatgaatta
      661 tagagtcgtt ctctatcagc cattgcaaca ttcaaattgt tcatttttag tttaaaaaaa
      721 actttatcaa tattatttgc tctaaatgga ccaaataagt aatatctggt tttaaacatg
      781 tatagaagaa acatctcatt aaaatcacca gtaaagaaat ggaaatcttt agttttttta
      841 atattatcag ttagtaactt tttataatca taaaaaaaat aagtagttct atctgagcga
      901 tactctttta taatactgaa ttcggaatta aaaaccacga taggtaactt taaacaaaca
      961 tgaagagact ttaataagac ttctttatct gaaaacactt ttgtttctct ctttcctttc
     1021 taca
//
LOCUS       DACCWK010000237          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCWK010000237 DACCWK010000000
VERSION     DACCWK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.247"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            2..>943
                     /locus_tag="IVW65_002747"
     rRNA            2..>943
                     /locus_tag="IVW65_002747"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACCWK010000238          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCWK010000238 DACCWK010000000
VERSION     DACCWK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.248"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            464..796
                     /locus_tag="IVW65_002748"
     CDS             464..796
                     /locus_tag="IVW65_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7901580.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKAVVQCKKYI
                     IQKDKVSEEMIYYTGKRKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          319 a          145 c          183 g          280 t
ORIGIN      
        1 tatgcttgtg atgaattgat tttcgttctg cacttttaga cgataaattg tgataattga
       61 aaatacaaaa aaagaaaggc ccctgtaagg ctcgggagac tggtaatctc cgtcagtgtc
      121 ctacaggaaa gcattaagat gacgccttaa tgcgtgccct tcagtgttac ggtattaccc
      181 gcaacggact aagtataaca gatattcaaa agtgatacca ctttttttgg ctctattaag
      241 gttaaaaata cgtgatattg taatgaatct atgcctaagc ataatgttat aaaaatgtcc
      301 gtgtggctct ttgccgtgtg acgaatgctg aaattagagg gaacgcatgt ttggatgcgt
      361 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
      421 aaaacttatt aaaaatattt aaagaaagtg agaggattat tttatgtcat atacaggaat
      481 tttgagtctc gaagatattt gtcattacgg taaacggtgt accgctacag agaaaatcac
      541 gaagaaacta tcaactgggc aaagcaaagc tgttgtgcag tgtaagaaat atataatcca
      601 gaaagacaaa gtttcagaag aaatgattta ttacactggg aaacgcaagc aaataatttt
      661 aaaagatcct atccctttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa
      721 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
      781 gcgtttaatc agctgaatta atcactctca taacttgtat tttatcgttt ttcgcttgct
      841 tgaaaatatt gtcgagactt ctaaaggaac ttagaatcat tagaaaaata aggagtagac
      901 ttgatctatc aacaattcct ttcgggg
//
LOCUS       DACCWK010000239          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCWK010000239 DACCWK010000000
VERSION     DACCWK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.249"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..355
                     /locus_tag="IVW65_002749"
     CDS             <1..355
                     /locus_tag="IVW65_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901581.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="IVW65_002750"
                     /pseudo
     CDS             615..910
                     /locus_tag="IVW65_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DACCWK010000240          826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCWK010000240 DACCWK010000000
VERSION     DACCWK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.250"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            192..290
                     /locus_tag="IVW65_002751"
                     /pseudo
     CDS             192..290
                     /locus_tag="IVW65_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(513..743)
                     /locus_tag="IVW65_002752"
     CDS             complement(513..743)
                     /locus_tag="IVW65_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP7901582.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          299 a          127 c          136 g          264 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg
      241 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag
      301 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
      361 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      421 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      481 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      541 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      601 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      661 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      721 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      781 tttcaagttg atcaatagct tccacgataa aaatatcttt agataa
//
LOCUS       DACCWK010000241          810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCWK010000241 DACCWK010000000
VERSION     DACCWK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.251"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            8..81
                     /locus_tag="IVW65_002753"
     tRNA            8..81
                     /locus_tag="IVW65_002753"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="IVW65_002754"
     tRNA            121..210
                     /locus_tag="IVW65_002754"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="IVW65_002755"
     tRNA            225..296
                     /locus_tag="IVW65_002755"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="IVW65_002756"
     tRNA            312..384
                     /locus_tag="IVW65_002756"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="IVW65_002757"
     tRNA            394..466
                     /locus_tag="IVW65_002757"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="IVW65_002758"
     tRNA            502..577
                     /locus_tag="IVW65_002758"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="IVW65_002759"
     tRNA            579..659
                     /locus_tag="IVW65_002759"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="IVW65_002760"
     tRNA            666..739
                     /locus_tag="IVW65_002760"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
BASE COUNT          178 a          184 c          223 g          225 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg gattgtggct
      781 ccggcattcg tgggttcgat tcccatcatt
//
LOCUS       DACCWK010000242          801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCWK010000242 DACCWK010000000
VERSION     DACCWK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.252"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IVW65_002761"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IVW65_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7901583.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          296 a          125 c          149 g          231 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac c
//
LOCUS       DACCWK010000243          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCWK010000243 DACCWK010000000
VERSION     DACCWK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.253"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            58..414
                     /locus_tag="IVW65_002762"
     CDS             58..414
                     /locus_tag="IVW65_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7901584.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            404..670
                     /locus_tag="IVW65_002763"
     CDS             404..670
                     /locus_tag="IVW65_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7901585.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT          307 a          104 c          158 g          207 t
ORIGIN      
        1 attcgaattt ttatgttata tactataata taaaatcaat atatgatata attgggtatg
       61 gaaaaaatat cttttgaaac atttaaaaga cctaatggtc atgatgaatt tttagaatgg
      121 ttggaaacat taccaaaaaa agatagtgcg aagcttttac gtacaataga agaaacagaa
      181 aagaatggga tgctagtagc tcaacggttg aaatgggtga aaaaattaga cactaatttg
      241 tacgaattaa gaagtaaggt aggatcaaac atccaaagag ctatctattt ccatgtggtc
      301 aatggaaggt atgtcattac ccatggattt actaaaaaaa cggagaaaac accaataagt
      361 gaacttaaac atgcaaaaga acttagagag gagtggttca caaatgatga ttaaaaaatt
      421 gattgaagaa aagcaaaaag acaaagattt tgcagaagca tatgaccgtg aaggaagaaa
      481 actcgaaact gcggttgctc tttatcatgc acgggaggaa gcaggcctaa ctcaagcgga
      541 gcttgcacaa aaagcacata caactcaatc tactattgct cgaattgaga gaggggataa
      601 cgtatctttc gataaacttg atgatatcgc tagggcatta ggcaaaaagc tggtagtaga
      661 atttgcttaa aagcgagttg aaaatatacc tgtaataggc tcctaactaa tcaattagtt
      721 tagtgggcct ttttgtacta aatagataaa agttgtccta tagtgtaaaa gtcaat
//
LOCUS       DACCWK010000244          739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCWK010000244 DACCWK010000000
VERSION     DACCWK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.254"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            140..346
                     /locus_tag="IVW65_002764"
     CDS             140..346
                     /locus_tag="IVW65_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901586.1"
                     /translation="MYADKNEIEWLLFESGISVKDIYKESGVARTTVIDLINKTSSID
                     KMRFDNAVKLTKMAKMRKNLYENK"
     gene            486..>739
                     /locus_tag="IVW65_002765"
     CDS             486..>739
                     /locus_tag="IVW65_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7901587.1"
                     /translation="MKLLNQKTVIAGTFAVTLAATLALAGSVDAASTNVMYRLYNPNN
                     HEHFYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYR"
BASE COUNT          260 a          136 c          124 g          219 t
ORIGIN      
        1 tagcaacaaa cctcatttta atacacgggg aacggatact cataaattcc cacctttcta
       61 tagaaacttt ttctcttgat attgtgattt attacaaaaa agagctataa taaagaaaaa
      121 atcaagaggg agcattgcaa tgtacgcaga taaaaatgaa atcgaatggt tattatttga
      181 aagtggaatc tccgttaaag atatatataa ggaatcaggt gtcgcgcgca ctacagttat
      241 cgacttgatt aataaaacct cctcaatcga taaaatgcgt tttgataatg cagtcaaact
      301 cactaaaatg gcgaaaatgc gaaaaaactt atatgaaaac aaataataag aaaccaatgc
      361 ttattcaaaa gtattggttt cttattgcat tttaatcggt ataccgtcat ttactcttga
      421 ctttgccggt ataccgacat ataattaact tgtaaaaaca tttaatctat ttaggagttg
      481 aaaacatgaa attattaaac caaaaaactg ttatagctgg aacatttgct gtcacactgg
      541 ctgccacact tgctcttgct ggatctgttg acgctgcttc aactaacgtc atgtatcgcc
      601 tttacaatcc aaataaccac gaacattttt acacctctag cacaaaagaa cgtgatcacc
      661 tagtgaaaat taagtggggt aattatgaag gccccgcttg ggaagcaccg actagcggtg
      721 gtcatcttgt ctatcgttt
//
LOCUS       DACCWK010000245          707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCWK010000245 DACCWK010000000
VERSION     DACCWK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.255"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..598
                     /locus_tag="IVW65_002766"
     CDS             <1..598
                     /locus_tag="IVW65_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7901588.1"
                     /translation="MKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVG
                     GGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKK
                     GQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLV
                     NGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          199 a          139 c          180 g          189 t
ORIGIN      
        1 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
       61 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      121 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      181 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      241 atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa aacgcttagg
      301 gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat
      361 tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg
      421 tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt
      481 caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga
      541 caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac
      601 gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca
      661 taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DACCWK010000246          699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCWK010000246 DACCWK010000000
VERSION     DACCWK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.256"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            131..343
                     /locus_tag="IVW65_002767"
     CDS             131..343
                     /locus_tag="IVW65_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901589.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            505..>699
                     /locus_tag="IVW65_002768"
     CDS             505..>699
                     /locus_tag="IVW65_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901590.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDIL"
BASE COUNT          249 a          104 c          122 g          224 t
ORIGIN      
        1 aaacactata tattccgaaa agtgagtatc tccattcata aaataatcaa catatttaaa
       61 aaatctaatt agctttttta gtcaatctaa agttctttat tctatgatat aatgttactg
      121 tagaaagttt gtggcggttt ggcaaatcca attgaaggag cgtggtgcct atgggcttat
      181 tagcttatcg gacgtactct gtagaaagga aatgcctgat ttgttctcat tggatcaaat
      241 cattgctatt atcggagcag cagcagtagt tattactgcc gtagcgaaag ctagcgattc
      301 gattttagct tggttaaagt tcaaaaaaga acaaaaaaaa tagagccgtc tcaacttttg
      361 gcgaggtaga cgagctctaa agctttattt acatgccatc cgctttgaac ggactacact
      421 gggcatgtga cagcatgccc tttcatttat attatatctc attcttttat ataatgaaag
      481 taaataaatc atggaggata aaatatggat aaatttaata gattaacatt aataaatcag
      541 tttgaaattt taaaagcttt aaatcctaac gaagaaaaat actatgctga aaaaatagaa
      601 atactaacag aaggttacga atatcactat agtgaaatat ttgaaaatat ttctgatcca
      661 ttgccagaag aagattcgcg gtttgttcta gacatatta
//
LOCUS       DACCWK010000247          680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCWK010000247 DACCWK010000000
VERSION     DACCWK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.257"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            139..>680
                     /locus_tag="IVW65_002769"
     CDS             139..>680
                     /locus_tag="IVW65_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7901591.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIY"
BASE COUNT          263 a          122 c          105 g          190 t
ORIGIN      
        1 tcactcttaa acaataagtt aaaaaaatac tgagagtata aaatattttg tgtaaatgaa
       61 aaaatccata caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac
      121 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
      181 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      241 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      301 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      361 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      421 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      481 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      541 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      601 gataagtatg tcgctatttt tatggacgct acttacattc cactaaaacg tcaaaccgta
      661 tccaaagaag cgatttatat
//
LOCUS       DACCWK010000248          653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCWK010000248 DACCWK010000000
VERSION     DACCWK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.258"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          216 a          103 c          168 g          166 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta cta
//
LOCUS       DACCWK010000249          651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCWK010000249 DACCWK010000000
VERSION     DACCWK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.259"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..406)
                     /locus_tag="IVW65_002770"
     CDS             complement(<1..406)
                     /locus_tag="IVW65_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901592.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLID"
     gene            complement(413..>651)
                     /locus_tag="IVW65_002771"
     CDS             complement(413..>651)
                     /locus_tag="IVW65_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901593.1"
                     /translation="DFNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRG
                     TYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          223 a           94 c           65 g          269 t
ORIGIN      
        1 tatctataag atctgttaga tatttttcaa attctaaaaa gctatccgaa ttttcttcga
       61 cgattaaata cttaagagaa tttatatcgg aaaagataga ttcaagttta ctaatctcat
      121 tttctaaatc tatattttca cgtgtgatta tctcatcact ttttcttaaa aagctaaaaa
      181 ttgcatcagt tgctttacac ttttccatta aagaagtata ttcttcaatt tttcttcttt
      241 ctttatggtc attttttaaa tcagtttcaa tgtctttttt catttgttct atactgagtt
      301 tgttcttttt gttcttttga gtaaatctaa aattcaaaca cgaaaaaagt atgttagcaa
      361 taacacctat aatagcaaat aaatcattta tactatttaa tttcatttat catcacaaac
      421 ttagaacttt acttatcatc tcataagaca agtttccaaa ttgataatta ttggtagatt
      481 ttaaatcatt ataattttcg agaaatttta tataagtacc tctcatcggt ttatggctat
      541 taaaagtgtt aaaatcacta tcttcaacta tttcttgaaa cctatttaat ttttcaataa
      601 aaaattgtgc ataaagtgct aacttaaatt cggtagagct attaaagtca a
//
LOCUS       DACCWK010000250          650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCWK010000250 DACCWK010000000
VERSION     DACCWK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.260"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>650
                     /locus_tag="IVW65_002772"
     CDS             <1..>650
                     /locus_tag="IVW65_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901594.1"
                     /translation="QEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNK
                     LYQTTTEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIR
                     KREQDLAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQT
                     PVNNQANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTAS"
BASE COUNT          277 a          112 c          131 g          130 t
ORIGIN      
        1 tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtatta aagaacgaaa
       61 agaagagttc taccgcggtt tacgtacaga taagctaaag attagcaaag aattaaatga
      121 acaactgaat aagctatatc aaacaacgac tgaagttctt atggaaaaac acgaacagat
      181 ccagcaagaa ttacaagtac aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca
      241 agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa aaagagcgtg aacaattaga
      301 aataagaaaa cgggaacagg atcttgcgga acaaaaaatg gcgatagaaa aacaacggtt
      361 agagcaagaa aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac
      421 attgcgtcaa gcacagttga attatctagc acaacctcaa ccagtagctg ctgttcaaac
      481 gccagtgaac aaccaagcaa atttaactcc ttcaccccag cgttccttta acggatggat
      541 ggtgggatgt attgcaagtt taagcttgct tctaggtgga ggaacagtat ttgcttttaa
      601 tcatatcaat caatcttcac aggctgattc tgtagccaca acggcgtcaa
//
LOCUS       DACCWK010000251          638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCWK010000251 DACCWK010000000
VERSION     DACCWK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.261"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>638
                     /locus_tag="IVW65_002773"
     CDS             <1..>638
                     /locus_tag="IVW65_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7901595.1"
                     /translation="YWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAEKQSSMVV
                     NDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLT
                     NTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSE
                     IGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL"
BASE COUNT          227 a          109 c          123 g          179 t
ORIGIN      
        1 atattggtac attcaaacag atactgttaa tacgtgtatt gttggtacct ctcgatcagg
       61 gaaaggtcag attgaagtgt tagccaccat tgacaattta tctcgtgcag aaaaacaaag
      121 ttcaatggtc gttaatgatc caaaaacaga attatatgtt ggtagtaaag atgaactaga
      181 agctagaggg tatgatgtat atgcctttaa tatcaccgat ccactgcaat caatgagtga
      241 taatccattg gcactcatcg tgaaatactg gaaacgagga gacatagata ctgcaacaca
      301 gttaaccaat actttcacaa atacgattta ttatgatgcc aacgcttcag ataacaaata
      361 ctttaacgaa aacgcgcaga aagctgtcaa tgcgcttatt tttgcgttgt tagaacaagc
      421 tgatcaaaca ggagattact ccaaattgac accgaataat ctcgttgagc ttctttctga
      481 gattggcggt tttaattatt atccagatcc agacaatgaa ttcaaacagt tgaatgcctt
      541 agatgaattt atgaatcagt tgccacctgg aaacattgca aaaaaacaat atggggcaac
      601 gaaaattgga tctgataaag cgaagggaaa tattttat
//
LOCUS       DACCWK010000252          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCWK010000252 DACCWK010000000
VERSION     DACCWK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.262"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>630
                     /locus_tag="IVW65_002774"
     rRNA            <1..>630
                     /locus_tag="IVW65_002774"
                     /product="16S ribosomal RNA"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DACCWK010000253          614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCWK010000253 DACCWK010000000
VERSION     DACCWK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.263"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            113..310
                     /locus_tag="IVW65_002775"
     CDS             113..310
                     /locus_tag="IVW65_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7901596.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            312..>614
                     /locus_tag="IVW65_002776"
     CDS             312..>614
                     /locus_tag="IVW65_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7901597.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKS"
BASE COUNT          237 a           59 c          113 g          205 t
ORIGIN      
        1 taaattctta ttcaggaatt attttgctac gtttcaagaa ggaattgttt tttgaaattt
       61 aatgaatgtt agaataatag tctaaaaaca ttaatttagg ggtgattaga ttatgaaaca
      121 tttaaaaatt ttgtctatta aagagacaca acttatctat gggggtacca ctcatagtgg
      181 aaaatattat ggaaatggag tgtattgcac taaaaataaa tgtacggtcg attgggccaa
      241 ggcaactact tgtattgcag gaatgtctat aggtggtttt ttaggtggag caattccagg
      301 gaagtgctaa aatgaaaaaa aatgctaagc aaattgttca tgaattatat aatgatatat
      361 ctataagtaa agatcctaaa tattctgata ttcttgaggt tttacaaaag gtatatttaa
      421 aattagaaaa acaaaaatat gaattagatc ccggtccttt aataaataga ttggtgaatt
      481 atctatattt tactgcttat actaataaaa taagattcac tgaatatcaa gaggaattaa
      541 taagaaattt gagtgaaatt ggaagaactg ctggaataaa tggtttatat cgagcagatt
      601 atggagataa atct
//
LOCUS       DACCWK010000254          610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCWK010000254 DACCWK010000000
VERSION     DACCWK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.264"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..160)
                     /locus_tag="IVW65_002777"
     tRNA            complement(76..160)
                     /locus_tag="IVW65_002777"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124..126),aa:Leu,seq:caa)
     gene            complement(178..249)
                     /locus_tag="IVW65_002778"
     tRNA            complement(178..249)
                     /locus_tag="IVW65_002778"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(215..217),aa:Gln,seq:ttg)
     gene            complement(259..331)
                     /locus_tag="IVW65_002779"
     tRNA            complement(259..331)
                     /locus_tag="IVW65_002779"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:His,seq:gtg)
     gene            complement(393..477)
                     /locus_tag="IVW65_002780"
     tRNA            complement(393..477)
                     /locus_tag="IVW65_002780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(441..443),aa:Leu,seq:caa)
     gene            complement(498..568)
                     /locus_tag="IVW65_002781"
     tRNA            complement(498..568)
                     /locus_tag="IVW65_002781"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(534..536),aa:Cys,seq:gca)
BASE COUNT          187 a          145 c          133 g          145 t
ORIGIN      
        1 tgatttggag gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg
       61 ctatattgat aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg
      121 attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg
      181 gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc
      241 tataccccaa gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa
      301 tccggtgcgt taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca
      361 ataaaaaact aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg
      421 ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag
      481 ttgatttatt agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac
      541 ctctgcctta ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa
      601 taaattatag
//
LOCUS       DACCWK010000255          592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCWK010000255 DACCWK010000000
VERSION     DACCWK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.265"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..510)
                     /locus_tag="IVW65_002782"
     CDS             complement(<1..510)
                     /locus_tag="IVW65_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP7901598.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPG"
BASE COUNT          168 a          114 c          103 g          207 t
ORIGIN      
        1 acctggaaaa gtagtttttg tttcaggtat ttgcggataa atctgttgga tttcttttaa
       61 ggtcattaat cgattatccc ctttttgtcc atctgctaga ggacgaaacc acatgtccat
      121 tttcaatccc ataagtacag ctggaaccac acaaactgtc gaaattgcta aacgtgtacc
      181 caaactagtt gtaggagtaa atggattcca aaaaccagct gaatcaaaat gaaagacctg
      241 tggcatttcc tgtaattgaa acagatttat tccttgtaag cttcccttaa caatttccaa
      301 tcggttgaaa acttctggta ctcttgttac taaatactct aaagggaggt atacaagtcc
      361 actataaaaa atctttttcg aaatgcgttt gtcatgtttt tctggtagct cttctcctga
      421 gggagtatag cgatagaaat gagaaagata cctattcttt tctaaattga atcgtttgtc
      481 ttttcgcttc gttgtttctc taacacgcat tatttcacct catttgattc tgaactttcg
      541 gaagagtttg attgtacagt tgaatcgctt gatccttctt tagccttact ag
//
LOCUS       DACCWK010000256          589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCWK010000256 DACCWK010000000
VERSION     DACCWK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.266"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..589
                     /locus_tag="IVW65_002783"
                     /pseudo
     CDS             1..589
                     /locus_tag="IVW65_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          228 a          102 c          112 g          147 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgcc
//
LOCUS       DACCWK010000257          568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCWK010000257 DACCWK010000000
VERSION     DACCWK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.267"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(165..455)
                     /locus_tag="IVW65_002784"
     CDS             complement(165..455)
                     /locus_tag="IVW65_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7901599.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          189 a           86 c           86 g          207 t
ORIGIN      
        1 attgacgctc cctttgcgag cagcaactag aataatttaa acattattaa actgtctata
       61 ttgttactaa taattccctt cttatagtgc attttcttag aaaaaaacaa tattacgata
      121 tcatgatatt aagatatcgt aatattatac aattgtgatt gatattatat atgttttttt
      181 atttttttat catgtttaat cttgaaggaa tcatattctt tttctaaaga caggagcttt
      241 tcataaacat cagacatgtt taaactagag gccaggctta aatcataaac gaatgaagca
      301 ggtaatgatt ttattctacg ttgtcgggtt atccagcttg caaccgttcc ttgtgggtag
      361 gcatataact cacaaaattg ttctacagtg attcctaaat tcttgataat gtaaacattg
      421 atcgggtgcg agtaaacaaa tgtattgata gacatggtgc gtaagcctac tttcttttat
      481 tattgcgttt gcaataatag tttaacacac gcattattct agtatctact cctatttgta
      541 aaaacaaaaa aggattcata gagataac
//
LOCUS       DACCWK010000258          555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCWK010000258 DACCWK010000000
VERSION     DACCWK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.268"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            404..>555
                     /locus_tag="IVW65_002785"
     CDS             404..>555
                     /locus_tag="IVW65_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7901600.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKK"
BASE COUNT          183 a          120 c          108 g          144 t
ORIGIN      
        1 ctctttttgt tttacataga aacaggaaaa gaccttttta ggaagacaca agtgccttct
       61 caattgaaaa acctttctct cactccaacc gccaccgccg ttcgagaaga aaggaagtga
      121 acgctgcacg ttcactaaga tatggtgtct tgcaaagcaa gacaaaatag acaaaacttc
      181 ctctcaccaa aaggttcgtt cctgttcgtt atctctttat tgatttgcca gaccggcaac
      241 aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt aaatggcacg ctgcgcgccc
      301 ttgtccttgt cgcttttcgt tgatttattc tggccaacaa atcaataaag aaagaactaa
      361 cgaaaggaag taaaaccttt cctaagagga ggcagaatca aacatgcgaa agaaatttga
      421 ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac atactgttga
      481 atgcgattgt ggagaaagag cgaaatgtat caaaagctat cgtttgtatc aatgtccaac
      541 atgcgggaaa aaata
//
LOCUS       DACCWK010000259          537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCWK010000259 DACCWK010000000
VERSION     DACCWK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.269"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..>537)
                     /locus_tag="IVW65_002786"
     CDS             complement(13..>537)
                     /locus_tag="IVW65_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7901601.1"
                     /translation="SYLAAVSEKVNLFTGREDIWHKFYQTLGEKSEQVLLGMKTFIFQ
                     RGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRLTLKADLSNGQMTFIWAFFAV
                     MMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEEFYVMEEAYDDMTYDDTSYEE
                     QPDSAHSSRIQRYH"
BASE COUNT          176 a          113 c           91 g          157 t
ORIGIN      
        1 attttgtgcc tatcaatgat agcgttggat cctggaagaa tgagcgctat ctggttgttc
       61 ctcataagat gtatcatcat aagtcatatc gtcatatgcc tcttccataa catagaattc
      121 ctccgcaact tctggttcat catatcgatg aacggccata gccaacagga tataaataat
      181 cggaacccat gggtatgaag tcaatgtatc ctccataaat gactgcatca tcaccgcaaa
      241 aaaggcccag ataaaagtca tctgcccgtt ggataaatct gctttcaacg ttaagcgacc
      301 aataaaaagg ataagcagca aagtagcaat cccaaatcct atcaatccat agatattcaa
      361 aatagaatta taactattgt ggtttcctaa aaattggttt cctcgttgga agataaatgt
      421 tttcattcct agtaaaactt gttcagattt ttctcctagc gtctgataga atttatgcca
      481 aatatcttct cgaccggtaa acaaattgac tttttcagag acagccgcca gataaga
//
LOCUS       DACCWK010000260          537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCWK010000260 DACCWK010000000
VERSION     DACCWK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.270"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>537
                     /locus_tag="IVW65_002787"
     CDS             <1..>537
                     /locus_tag="IVW65_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP7901602.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRTASTT"
BASE COUNT          185 a           96 c          104 g          152 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccac
//
LOCUS       DACCWK010000261          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCWK010000261 DACCWK010000000
VERSION     DACCWK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.271"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..368
                     /locus_tag="IVW65_002788"
     CDS             <1..368
                     /locus_tag="IVW65_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901603.1"
                     /translation="LKDRAKKLSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQA
                     YYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGV
                     KKNFFENCWEFFAQLFFDN"
BASE COUNT          197 a           72 c           93 g          172 t
ORIGIN      
        1 aattgaaaga tcgagcaaaa aaactttcat acgaccagtt gaaaaaagtg gctgaaggag
       61 aaaccaaaac gattgatgat caggaaggcg tgattgaatt ttccattgaa gtacctgcaa
      121 acaaaaaaca agcatactat atcgttaatg aatcaagccc tgaaaacatt tcaaacagtg
      181 aagatattgt tttattgaca cctgtttcaa atgaaaaagg tgaatttttg acggatgttt
      241 ggttataccc taaatcagag aaatcagagc cgaaagaaga aattaaaaaa gttgtgtcaa
      301 ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt ttttgctcaa ttattttttg
      361 ataattagga tgaaatgatg aaaataataa gaatattagt ttttaatcta tcctttttag
      421 ccgcaacaat attgtgttgc ggtttaattt tttctcaatt atcaatttat ccaagatatc
      481 aacgattgtt cattcatttt ctaaacattc aatgtgcttc catgccatta gagg
//
LOCUS       DACCWK010000262          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCWK010000262 DACCWK010000000
VERSION     DACCWK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.272"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..132)
                     /locus_tag="IVW65_002789"
     CDS             complement(<1..132)
                     /locus_tag="IVW65_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7901604.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACCWK010000263          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCWK010000263 DACCWK010000000
VERSION     DACCWK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.273"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..342
                     /locus_tag="IVW65_002790"
     CDS             <1..342
                     /locus_tag="IVW65_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901605.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           68 c           93 g          143 t
ORIGIN      
        1 gataacatac gagataaaaa ggcaagttta aaatcactaa aagatacatg gcttgattta
       61 tcagaagata atccatacag ccaattctta attactgtaa tggctggtgt taaccaatta
      121 gagcgagatc ttattcggat gagacaacgt gaagggattg aattggctaa gaaagaagga
      181 aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa ttatgcggta
      241 aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct
      301 agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta
      361 atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat
      421 gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt
      481 atgtaaatgc ttataaattc ggccctataa
//
LOCUS       DACCWK010000264          501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCWK010000264 DACCWK010000000
VERSION     DACCWK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.274"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..424)
                     /gene="spxA"
                     /locus_tag="IVW65_002791"
     CDS             complement(26..424)
                     /gene="spxA"
                     /locus_tag="IVW65_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7901606.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          142 a          103 c           85 g          171 t
ORIGIN      
        1 cttttccaag tttcgtatta tccttctata atccagccat caattgtgct tgacgtaatt
       61 ctaattgtcg tacatcgcgc ggtaggaacc gacgaatttc atcttcatta aaaccaactt
      121 gcagacgttt atcatcaatc atgattggac ggcgaagcaa tccaggattg ttttgcacaa
      181 gttccaataa ttcttgtaac ggcagatcat ctaaatccat gttcagtttt tggaatactt
      241 tggaacgtgt cgagatgatt tcttctgttc cgtcttctgt catttgtaaa atcgctttta
      301 attcagaact gttcaatggt tctgaaaaaa tattacgttc aacaaatgga atctgatgtt
      361 cttgcaacca cgcacgagcc tttctgcagg aagtacaact tggggaagta tataatgtta
      421 acaacgtgca ccactccttt tcgtgataag aatcactttt ttgtaacaga agatcataat
      481 aatctcccat ctgtttctat t
//
LOCUS       DACCWK010000265          494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCWK010000265 DACCWK010000000
VERSION     DACCWK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.275"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          184 a           73 c           87 g          150 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atcgccgatt gtaaaattaa
      361 gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat
      421 aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag
      481 tcttatttca aaat
//
LOCUS       DACCWK010000266          490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCWK010000266 DACCWK010000000
VERSION     DACCWK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.276"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            149..388
                     /locus_tag="IVW65_002792"
     CDS             149..388
                     /locus_tag="IVW65_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7901607.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            437..>490
                     /locus_tag="IVW65_002793"
     CDS             437..>490
                     /locus_tag="IVW65_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7901608.1"
                     /translation="MLVFQMRNVDKTSTVLKQ"
BASE COUNT          204 a           58 c           94 g          134 t
ORIGIN      
        1 tctttctcaa aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat
       61 cacttatcac aaatcactta tcacaaatca caagtgattt gtgattgttg atgataaaat
      121 aagaataaga agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaaga
      181 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
      241 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
      301 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcatta
      361 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa
      421 gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt
      481 tttgaaacag
//
LOCUS       DACCWK010000267          489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCWK010000267 DACCWK010000000
VERSION     DACCWK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.277"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..414)
                     /locus_tag="IVW65_002794"
     CDS             complement(<1..414)
                     /locus_tag="IVW65_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901609.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          186 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacata
//
LOCUS       DACCWK010000268          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCWK010000268 DACCWK010000000
VERSION     DACCWK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.278"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>487)
                     /locus_tag="IVW65_002795"
     CDS             complement(<1..>487)
                     /locus_tag="IVW65_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7901610.1"
                     /translation="GFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLG
                     FFGGSRFIPIVSSLAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRL
                     TGAVGLHHTIYPLFWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFA
                     G"
BASE COUNT          184 a           97 c           80 g          126 t
ORIGIN      
        1 cttcctgcaa aaaatctagt accataagta aacagaccag tgtggtttgg atcagctagt
       61 tgggcaaaga aaatattttg ggctccagca attgttgatc cagctacagt ttcggtacca
      121 cctaaagatg tataccagaa caaagggtaa atggtgtggt gtagtccaac agcacctgtt
      181 aaacgcaata aaaaaccata taaaaaagtt cctaaactac ccatttgggc aatttgttca
      241 ccagcaacta ctaaaccatt ttgaattggt ggccaaatta aataaaagaa agaaccaata
      301 atgattgcag ctaaactaga aacaatcgga ataaatcttg atcctccaaa aaaacctaag
      361 aattgtggta attcaatttt tctataacga ttgtgtaaaa atgcaacagt accaccaaca
      421 acaattgatc ccactacacc tgtatctagt gttgcatctt ttgctgaaaa aagttcaaga
      481 aacccac
//
LOCUS       DACCWK010000269          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCWK010000269 DACCWK010000000
VERSION     DACCWK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.279"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>487)
                     /locus_tag="IVW65_002796"
     CDS             complement(<1..>487)
                     /locus_tag="IVW65_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP7901611.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACCWK010000270          477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCWK010000270 DACCWK010000000
VERSION     DACCWK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.280"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            5..>477
                     /locus_tag="IVW65_002797"
     CDS             5..>477
                     /locus_tag="IVW65_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7901612.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQS"
BASE COUNT          170 a           65 c           91 g          151 t
ORIGIN      
        1 cattatggaa aataaaattc attgcactac gggaagagta attactttag tgttgttttt
       61 gttccttctt ctttttgcga ggggaactgc agtccaggcc ttagaaaaag ttgatccgag
      121 tcaaatcaaa gaaaataata atctggtgct ttatcatgat agtgattcat tgagaaatat
      181 cgaagtaact gggatcaatg gatttaagat actagatgag tattcaacta aaaaacaaaa
      241 agtacttgct tggccaaaag tcaaagattt gaaaagtacg tcaaaaatcg ttgcccatta
      301 tcaaaaagta ggtacttatc taggaaaaga aattgattgt gacgtgactt tttctaattt
      361 tgtagcgcac gctggggtta accaagacgg attagctaaa acaattaatg gcaaaaagta
      421 ttataattat ttgctctttg ataaaaatat ttatcgagga tttttcatgc agtcaat
//
LOCUS       DACCWK010000271          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCWK010000271 DACCWK010000000
VERSION     DACCWK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.281"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>468
                     /locus_tag="IVW65_002798"
     CDS             <1..>468
                     /locus_tag="IVW65_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7901613.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACCWK010000272          464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCWK010000272 DACCWK010000000
VERSION     DACCWK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.282"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..430)
                     /locus_tag="IVW65_002799"
     CDS             complement(<1..430)
                     /locus_tag="IVW65_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7901614.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          133 a           92 c           59 g          180 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tcta
//
LOCUS       DACCWK010000273          456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCWK010000273 DACCWK010000000
VERSION     DACCWK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.283"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            22..>456
                     /locus_tag="IVW65_002800"
     CDS             22..>456
                     /locus_tag="IVW65_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7901615.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRN"
BASE COUNT          168 a           85 c           92 g          111 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaat
//
LOCUS       DACCWK010000274          456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCWK010000274 DACCWK010000000
VERSION     DACCWK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.284"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(73..318)
                     /locus_tag="IVW65_002801"
     CDS             complement(73..318)
                     /locus_tag="IVW65_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901616.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          124 a           90 c           61 g          181 t
ORIGIN      
        1 tagtttttgg tgtatttcat tggtccaagc taaaaaactc ttagaagcga ttttagagcc
       61 atttaaaccc acttaattat attctccatg aaattcgctc catgcgtcaa aagctgcgtc
      121 tgaaattccg ccgtcttcgt tcactagttg cattaatttt tccaacgatc gttttctttc
      181 gggtgtcata tttttatatt ccaacaattt ttcgtctgtt aaatcgaact tgattgcttg
      241 aattgcttgc tctaattttt ctaagttttg aaaatattcg taaggaaatt tttccatttt
      301 ttctttctcc agtctcacaa tttttctaga aataaaatca cttctttgcc gtctatactc
      361 tcaaaaaaaa tgtcgtttga aaaactaaat aaatttttga tcgtgcattt ttaaatttgg
      421 ttatcgatta cacgtgcagc actccccccc gaattt
//
LOCUS       DACCWK010000275          453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCWK010000275 DACCWK010000000
VERSION     DACCWK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.285"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>453
                     /locus_tag="IVW65_002802"
     CDS             <1..>453
                     /locus_tag="IVW65_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP7901617.1"
                     /translation="AQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNDKKVQEHYAIIPTKT"
BASE COUNT          159 a           96 c           93 g          105 t
ORIGIN      
        1 cgctcaaaat ggcatatttg atgcgaagct tgatcccaat gaaacccaag cgacgcaaga
       61 agcatttgaa gcctatttaa aagaaaaagg tgttcaagta ggaaagcagc caggcacgat
      121 tcgccaagtt gaaaccgaga aaaaaagcgc ggcaagtcca cgtctttttt ctctatctag
      181 ccttcagtct aaaatgaatc aactcatgaa agctagtgcc aaagacactt tggaagccat
      241 gcaagggttg tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac
      301 ggaaggggaa tatgcgtatc tgttggatca tttagacgaa tacaagcatt ttttaaaggc
      361 cgaatcaatt ccaaccccta ttcatacgcc gaatagtcgg tatgtgaacg ataagaaagt
      421 ccaggaacat tacgcaatca ttcctacaaa aac
//
LOCUS       DACCWK010000276          443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.286, whole genome shotgun sequence.
ACCESSION   DACCWK010000276 DACCWK010000000
VERSION     DACCWK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.286"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>443
                     /locus_tag="IVW65_002803"
     CDS             <1..>443
                     /locus_tag="IVW65_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7901618.1"
                     /translation="QVDSAKNKLDSAYKGLKKKPAPTVNKAELQALYNRVKSTAKGDF
                     TEESWNNFQTALSNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYMVTIN
                     HIDNQTGQVIATDQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIG"
BASE COUNT          177 a           75 c           77 g          114 t
ORIGIN      
        1 caagtggata gtgccaaaaa taaattagat tctgcttaca aaggattaaa gaaaaaacca
       61 gcacctactg tgaataaagc tgaattacaa gctttataca accgagtaaa atcaactgct
      121 aaaggagatt ttacagaaga atcttggaat aattttcaga ctgctttatc caatgctaaa
      181 aaagtactag acgattctaa ggcttctcaa gcccaagtaa ataatgctaa aaatagtcta
      241 gatgctgcat ttaaagggtt gcaacacaaa cctgaaacgc caaaagaata tatggttacc
      301 attaaccata ttgacaatca aacaggtcaa gtgattgcta cagatcaagc aaaagtgact
      361 tctggttcta cttacactgc taaggcaaaa gcatttaaat atagcgaagg tgctaaagat
      421 aattttagct acaaagtgat tgg
//
LOCUS       DACCWK010000277          427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCWK010000277 DACCWK010000000
VERSION     DACCWK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.287"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(7..336)
                     /locus_tag="IVW65_002804"
     CDS             complement(7..336)
                     /locus_tag="IVW65_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901619.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          129 a           85 c           41 g          172 t
ORIGIN      
        1 ttcggatcat aatttttcat ttgttgtaac ctcctcataa tcagttagta ctataaaggt
       61 atctttgttt ttatccaaaa aatctaataa atcttttgta gaagatatct cttcatcaac
      121 ttcaccataa taaatacctt cgataattgt tgctatatct gaaaaactaa tttttactgg
      181 acatttatcg tctaccgcat tcaataccga caaaattgct tctttttctg caatctctcg
      241 tttgaatttc tttggagcta atcgcatgac actcaagtca ttttcaagct tttcaatcca
      301 tgcagtttta ctcattttcg caaatttttg aatcattcct ctaccacctt tgctatctac
      361 taaattagtt ttacttcata acgaatatct ttatcgtttt tcgttcactc aaaaatatat
      421 aaaataa
//
LOCUS       DACCWK010000278          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.288, whole genome shotgun sequence.
ACCESSION   DACCWK010000278 DACCWK010000000
VERSION     DACCWK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.288"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            19..303
                     /locus_tag="IVW65_002805"
     CDS             19..303
                     /locus_tag="IVW65_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901620.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          165 a           71 c           65 g          125 t
ORIGIN      
        1 tgcaaaagga gattttttat ggacatttta gttcggaata ttgatccaaa gtttgttgca
       61 acaatagaca aacgttgcca agaactcacg aataaaacag gagaaaaatg gagtcgaaat
      121 aaatatctaa aagttttaat cgaaagtgat tttgaccgtc aacttacgac ttacaaaaaa
      181 gaccaattcg atttattgct cgataaattt attgctatcc aaacagaaaa tactcaaata
      241 ttaagcgagt atgtctacac aaataatcaa atcattgaaa tgcttatcgg aggaatgata
      301 taaccatgag aaaaacttct gtccaaaaac atgttacttt gtaccctagt catgttgacg
      361 ttttagattc catcatcgaa aaaaatccag atatcaataa ctactcggaa gccattcgct
      421 ttttat
//
LOCUS       DACCWK010000279          421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCWK010000279 DACCWK010000000
VERSION     DACCWK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.289"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(170..355)
                     /locus_tag="IVW65_002806"
     CDS             complement(170..355)
                     /locus_tag="IVW65_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP7901621.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DACCWK010000280          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCWK010000280 DACCWK010000000
VERSION     DACCWK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.290"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          143 a           71 c           65 g          136 t
ORIGIN      
        1 gaaagacatt tgggttaaat tgtgagataa taataaagaa gagtttaaag cgcaccccaa
       61 accacttccc cataagtgtg ttacgcttta aactctttta tatttgaagc cattaaaaag
      121 cataccatat ttttgaaaaa aagtgagaaa aaaggcttac aattggagtg gtagttaatt
      181 agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc atcatattac
      241 acaatcttaa tactaactta aaaaatatct cctttcacaa gtatggtgat aaaattcgtt
      301 ccgggctacc tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt
      361 atactttata tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtc
//
LOCUS       DACCWK010000281          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.291, whole genome shotgun sequence.
ACCESSION   DACCWK010000281 DACCWK010000000
VERSION     DACCWK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.291"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..324
                     /locus_tag="IVW65_002807"
     CDS             <1..324
                     /locus_tag="IVW65_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901622.1"
                     /translation="GLVNNLKIIDDTTWEYSESVWHPDPVQISVKRQKDYKGLERYKI
                     VDSAGISDFINKPDSLFIVVPYEKDGVKKMVFLGSSKDEKRTKEKLIHDGSQSNKYKL
                     QKTSE"
     gene            321..>415
                     /locus_tag="IVW65_002808"
     CDS             321..>415
                     /locus_tag="IVW65_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901623.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILER"
BASE COUNT          162 a           57 c           79 g          117 t
ORIGIN      
        1 ggtcttgtta ataatttgaa aattatagac gatactacat gggaatattc tgaaagtgta
       61 tggcatccag atcctgtaca aatatcagtt aagagacaaa aagactataa aggtttagaa
      121 agatacaaaa tagtagattc tgcaggtatt agcgacttta tcaataaacc agattcttta
      181 tttatcgttg ttccttacga gaaagatggc gtaaaaaaaa tggtttttct tggatctagt
      241 aaggatgaaa aacgaacgaa agagaaatta attcatgatg gatcacaatc aaataaatac
      301 aaattacaaa agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca
      361 cagtggcaaa agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacg
//
LOCUS       DACCWK010000282          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCWK010000282 DACCWK010000000
VERSION     DACCWK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.292"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..339)
                     /locus_tag="IVW65_002809"
     CDS             complement(<1..339)
                     /locus_tag="IVW65_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7901624.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACCWK010000283          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.293, whole genome shotgun sequence.
ACCESSION   DACCWK010000283 DACCWK010000000
VERSION     DACCWK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.293"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>404
                     /locus_tag="IVW65_002810"
     CDS             <1..>404
                     /locus_tag="IVW65_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901625.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACCWK010000284          396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.294, whole genome shotgun sequence.
ACCESSION   DACCWK010000284 DACCWK010000000
VERSION     DACCWK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.294"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          135 a           59 c           49 g          153 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaaattt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gtttta
//
LOCUS       DACCWK010000285          391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.295, whole genome shotgun sequence.
ACCESSION   DACCWK010000285 DACCWK010000000
VERSION     DACCWK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.295"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            2..370
                     /locus_tag="IVW65_002811"
     CDS             2..370
                     /locus_tag="IVW65_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7901626.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          127 a           59 c           87 g          118 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt t
//
LOCUS       DACCWK010000286          389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.296, whole genome shotgun sequence.
ACCESSION   DACCWK010000286 DACCWK010000000
VERSION     DACCWK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.296"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>389
                     /locus_tag="IVW65_002812"
     CDS             <1..>389
                     /locus_tag="IVW65_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7901627.1"
                     /translation="EQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQIQLKA
                     IGKVPVDLGWKKLFGKESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEG
                     TLITAMKTAGKTVDSEEAQSILKEVEG"
BASE COUNT          153 a           66 c           97 g           73 t
ORIGIN      
        1 gaacaactaa gtccactgca gcaggctatt tatgaacaag tgttaaaaac aaccgtggcg
       61 atgtttgcgg aaaaatatac ctatgaagaa acaacgatct tgacgcaagt tcagcagatt
      121 cagttaaaag caatcggaaa agtgccggta gatctaggtt ggaagaagtt atttggaaaa
      181 gagagcgacg gaaaagagaa ggaagaagag ccgctacttc ctaaagtgac aaaaggagaa
      241 atggtgacgg tagatctaca agtccttgaa aaagaaacca agccaccaca accttatacc
      301 gaaggtacgt tgatcactgc gatgaaaaca gctgggaaaa ccgtggatag tgaagaagcg
      361 caatccatat tgaaagaagt agaaggaat
//
LOCUS       DACCWK010000287          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.297, whole genome shotgun sequence.
ACCESSION   DACCWK010000287 DACCWK010000000
VERSION     DACCWK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.297"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..276
                     /locus_tag="IVW65_002813"
     CDS             <1..276
                     /locus_tag="IVW65_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP7901628.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
BASE COUNT          131 a           66 c           79 g          105 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat g
//
LOCUS       DACCWK010000288          376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.298, whole genome shotgun sequence.
ACCESSION   DACCWK010000288 DACCWK010000000
VERSION     DACCWK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.298"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>376
                     /locus_tag="IVW65_002814"
     CDS             <1..>376
                     /locus_tag="IVW65_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP7901629.1"
                     /translation="GMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHK
                     IRPYPIFNHGETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDE
                     YARNLYHGLHDQLEEKNEENMFY"
BASE COUNT          131 a           60 c           62 g          123 t
ORIGIN      
        1 ggtatgaata ctcatatcaa tcgcaatgtt gattcagatc gtatcttaac tcctgaaaga
       61 atttctactt ttttagaagg tgaaacgatt gtactcgctc ctttaaaacg acaagatttg
      121 aaaggtcata aaatacggcc gtatcctatt tttaatcatg gggaaacgaa tcttccttat
      181 gcttatcaat atattgaaga tttcaatcca tcgtgggata tcaatgatat agacatacat
      241 tgtacacatg cggacttgga tcttaaggct ttacaaattg attatgatta tttcctacca
      301 gatgaatatg ctagaaatct ctatcatgga ctgcatgatc aattagaaga aaaaaacgaa
      361 gaaaatatgt tttata
//
LOCUS       DACCWK010000289          362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.299, whole genome shotgun sequence.
ACCESSION   DACCWK010000289 DACCWK010000000
VERSION     DACCWK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.299"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>362)
                     /locus_tag="IVW65_002815"
     rRNA            complement(<1..>362)
                     /locus_tag="IVW65_002815"
                     /product="16S ribosomal RNA"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DACCWK010000290          358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.300, whole genome shotgun sequence.
ACCESSION   DACCWK010000290 DACCWK010000000
VERSION     DACCWK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.300"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            82..>358
                     /locus_tag="IVW65_002816"
     CDS             82..>358
                     /locus_tag="IVW65_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7901630.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTC"
BASE COUNT          126 a           57 c           74 g          101 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgta
//
LOCUS       DACCWK010000291          348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.301, whole genome shotgun sequence.
ACCESSION   DACCWK010000291 DACCWK010000000
VERSION     DACCWK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.301"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(89..>348)
                     /locus_tag="IVW65_002817"
     CDS             complement(89..>348)
                     /locus_tag="IVW65_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7901631.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          106 a           54 c           64 g          124 t
ORIGIN      
        1 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaattccag actcctgtgt
       61 aaaatgctat acaatgtttt taccatttct acttatcaaa attgatgtat tttcttgaag
      121 aataaatcca ttcatcatgt aggtccataa gaacggctcc aattaagcga ttggctgatg
      181 tttgattggg gaagatgcga ataatctttt ctcttctgcg tacttcttga ttcagtcgtt
      241 caattagatt ggtactcttt agtcgattgt gggaatttcc ttgtacggta tattgaaagg
      301 cgtcttcgaa tccatcatcc aatgatgcgc aagcttttga atattttg
//
LOCUS       DACCWK010000292          347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.302, whole genome shotgun sequence.
ACCESSION   DACCWK010000292 DACCWK010000000
VERSION     DACCWK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.302"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>347
                     /locus_tag="IVW65_002818"
     CDS             <1..>347
                     /locus_tag="IVW65_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7901632.1"
                     /translation="IWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKL
                     QSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKN
                     RRNGTLFGFSVST"
BASE COUNT          117 a           72 c           70 g           88 t
ORIGIN      
        1 atatctggtt acgaaagaaa cgggatacgc aagcagccta tgctttctta aaacgactcc
       61 ataaacagtt tggtgagccg aaagcaattg tgaccgataa agcaccttct cttggctccg
      121 cctttagaaa gttacagagt gtgggtttat atactaagac agagcaccga actgtgaagt
      181 atcttaacaa tttaatagaa caagaccatc gacctattaa acgacgaaat aaattttatc
      241 aaagtctccg tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata
      301 aaaagaaccg aagaaatgga acgctcttcg gcttttcggt gtctact
//
LOCUS       DACCWK010000293          323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.303, whole genome shotgun sequence.
ACCESSION   DACCWK010000293 DACCWK010000000
VERSION     DACCWK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.303"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>323
                     /locus_tag="IVW65_002819"
     CDS             <1..>323
                     /locus_tag="IVW65_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7901633.1"
                     /translation="TGGPFVWNATYKNSVRLSTNTLLLGMMGAGKSTVLKMIAEAHLA
                     AGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIFATRTYDEASSLYDDGSV
                     KDLKI"
BASE COUNT           97 a           53 c           79 g           94 t
ORIGIN      
        1 acaggcggcc catttgtctg gaatgcgacg tataaaaata gtgtacggtt gtctactaat
       61 accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat gatcgcggaa
      121 gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga ctttattcct
      181 ttcttaaagg aatataacgg tattatggtg cgtttagacg gatctgacgg aatgatcaat
      241 cctttagaga tatttgccac acggacgtat gatgaagcat ccagtttata tgacgatgga
      301 tcagtcaaag atttgaagat caa
//
LOCUS       DACCWK010000294          323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.304, whole genome shotgun sequence.
ACCESSION   DACCWK010000294 DACCWK010000000
VERSION     DACCWK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.304"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            44..>323
                     /locus_tag="IVW65_002820"
     CDS             44..>323
                     /locus_tag="IVW65_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901634.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT          123 a           37 c           55 g          108 t
ORIGIN      
        1 cttataattt tttgttaatg aacaagatag gaagaaacat ttaatggaca gttttcagag
       61 attgtcacta gtaaatcaat ttgagttatt ggctaaattt gaggaccaaa acagatctta
      121 ttatgaaaga aaaatcgaaa tattgagaga aggttacgag tatcattatg atgatgaaat
      181 ttggagtgat ctttctgagc catttccgaa agaagattct cgatttgtat tagatgtatt
      241 gaacatgtat cgagatatca attttagtaa atcaaagcta aacttagatg aaaaccaaat
      301 tactaattca tactatactc att
//
LOCUS       DACCWK010000295          319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.305, whole genome shotgun sequence.
ACCESSION   DACCWK010000295 DACCWK010000000
VERSION     DACCWK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.305"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            76..>319
                     /locus_tag="IVW65_002821"
     CDS             76..>319
                     /locus_tag="IVW65_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7901635.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
BASE COUNT          134 a           51 c           54 g           80 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaag
//
LOCUS       DACCWK010000296          316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.306, whole genome shotgun sequence.
ACCESSION   DACCWK010000296 DACCWK010000000
VERSION     DACCWK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.306"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>316
                     /locus_tag="IVW65_002822"
     CDS             <1..>316
                     /locus_tag="IVW65_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7901636.1"
                     /translation="NDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDT
                     ALLWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKY
                     KSF"
BASE COUNT          116 a           62 c           55 g           83 t
ORIGIN      
        1 aatgatcatt tcaccgatgt tgtaggagct gatacttatc acagaagtgg tgtaacgtat
       61 acgcttcaag gcgcttcccc aacattcatg attggcgcaa atacgaatag tatgatgttt
      121 agctttgata ctgcattgct atggacacca caaccatcga agcctacaaa agaagtgttt
      181 aacaaagcta atactgaaga ggcagcacac aatattgaca aaaaagtgat tccacaagga
      241 tcagatgttt actatcatat tcaccaaaag tttgatgcat taacagtcaa cacaatgaac
      301 aaatacaaat cattta
//
LOCUS       DACCWK010000297          298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.307, whole genome shotgun sequence.
ACCESSION   DACCWK010000297 DACCWK010000000
VERSION     DACCWK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.307"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..180
                     /locus_tag="IVW65_002823"
     CDS             <1..180
                     /locus_tag="IVW65_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901637.1"
                     /translation="LKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFEQLRRT
                     YVKKSSNCQLLLEVS"
BASE COUNT          113 a           31 c           56 g           98 t
ORIGIN      
        1 ctaaaaacga tgaaacaaaa acaccttaaa agattatctg tcatgcaata tatcaatgat
       61 atgcagataa caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa ctttgaacag
      121 ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac tattgttaga agtctcatga
      181 cattcattaa tagaattcat ttttaaaact ttttctatgg aaatgagaaa aagatttttt
      241 gttttgtctg aaaggaggta gctggatgag aaaatggtta tttggttttt taggagtc
//
LOCUS       DACCWK010000298          276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.308, whole genome shotgun sequence.
ACCESSION   DACCWK010000298 DACCWK010000000
VERSION     DACCWK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.308"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          105 a           28 c           36 g          107 t
ORIGIN      
        1 caatattttt tgtaaataaa tgtcaatcaa gaaaatttaa acgattatga cattaaaaat
       61 atattttatg aattttctag tatataactt gatttatctg ttaagattta ttaagaaaac
      121 gttttcttag ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta
      181 ggtattttaa ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt
      241 acaaatgaac caactactgt agtagataat aaccaa
//
LOCUS       DACCWK010000299          255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.309, whole genome shotgun sequence.
ACCESSION   DACCWK010000299 DACCWK010000000
VERSION     DACCWK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.309"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>255
                     /locus_tag="IVW65_002824"
     CDS             <1..>255
                     /locus_tag="IVW65_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7901638.1"
                     /translation="YEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKD
                     YGWKYEGIGWYGADTSKPKVNKSALEALYTSVKGTKKGDY"
BASE COUNT           83 a           54 c           57 g           61 t
ORIGIN      
        1 cttatgaagg gcctgcctgg cgttctgccg aaaaaaataa taaaccgatc taccgccttt
       61 tccttcctgg tgtaaaatct ggctcacatc actacactgc aagttggaaa gaagtacaat
      121 ggttaaccaa agactatggc tggaaatacg aaggtatcgg ttggtatggc gctgacacct
      181 ctaaacccaa agtgaataaa tcggctctag aagctcttta tacgagcgtt aagggaacga
      241 aaaaaggtga ctata
//
LOCUS       DACCWK010000300          242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.310, whole genome shotgun sequence.
ACCESSION   DACCWK010000300 DACCWK010000000
VERSION     DACCWK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.310"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT           78 a           49 c           25 g           90 t
ORIGIN      
        1 gactcctaaa aaaccaaata accatttttt catccatctg cctcctttca gacaaaacaa
       61 aaaaactttt tctcatttcc atagaaaaag ttttaaaaat gaattctata agttcgtctc
      121 aactgttcaa agtttttcat tgaacctaac aaacaagctt tctcttttcc tgttaactgc
      181 atatcattga tatattgcgc gacagataat cttttaaggt gtttttgctt catcgttttt
      241 ag
//
LOCUS       DACCWK010000301          237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.denovo.311, whole genome shotgun sequence.
ACCESSION   DACCWK010000301 DACCWK010000000
VERSION     DACCWK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.denovo.311"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(108..>237)
                     /locus_tag="IVW65_002825"
     CDS             complement(108..>237)
                     /locus_tag="IVW65_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7901639.1"
                     /translation="WWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT           58 a           61 c           55 g           63 t
ORIGIN      
        1 gacaaatttt ataaggtgca gaacttcttt ccgtatgcta ttccgattgt ccttgacaat
       61 gagcctcctc ggatgtgacg atcttcggca ggagctaaca gttaaagtta gactgcctcg
      121 ctagcgttag cacatccgag ctcattatca aggattcgct gcgccaatct ctggttacgg
      181 taaccaaccg gtgtctcgta gccaagtgta ccgtgcaacc gaaggtggtt ccaccaa
//
LOCUS       DACCWK010000302        12266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.072, whole genome shotgun sequence.
ACCESSION   DACCWK010000302 DACCWK010000000
VERSION     DACCWK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.072"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(134..577)
                     /locus_tag="IVW65_002826"
     CDS             complement(134..577)
                     /locus_tag="IVW65_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7901640.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IVW65_002827"
     CDS             complement(795..2135)
                     /locus_tag="IVW65_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7901641.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IVW65_002828"
     CDS             2404..3300
                     /locus_tag="IVW65_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7901642.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IVW65_002829"
     CDS             complement(3450..4391)
                     /locus_tag="IVW65_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7901643.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IVW65_002830"
     CDS             4517..5638
                     /locus_tag="IVW65_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7901644.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IVW65_002831"
     CDS             5896..7398
                     /locus_tag="IVW65_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7901645.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IVW65_002832"
     CDS             7465..7992
                     /locus_tag="IVW65_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7901646.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IVW65_002833"
     CDS             8203..9264
                     /locus_tag="IVW65_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7901647.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IVW65_002834"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IVW65_002834"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7901648.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IVW65_002835"
     CDS             10177..12159
                     /locus_tag="IVW65_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7901649.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
BASE COUNT         4063 a         2245 c         2557 g         3401 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcaca
//
LOCUS       DACCWK010000303        12085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.075, whole genome shotgun sequence.
ACCESSION   DACCWK010000303 DACCWK010000000
VERSION     DACCWK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.075"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            20..2098
                     /locus_tag="IVW65_002836"
     CDS             20..2098
                     /locus_tag="IVW65_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7901650.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2258..4144
                     /locus_tag="IVW65_002837"
     CDS             2258..4144
                     /locus_tag="IVW65_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7901651.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4156..5103
                     /locus_tag="IVW65_002838"
     CDS             4156..5103
                     /locus_tag="IVW65_002838"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7901652.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5123..5650
                     /locus_tag="IVW65_002839"
     CDS             5123..5650
                     /locus_tag="IVW65_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7901653.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(5713..6366)
                     /locus_tag="IVW65_002840"
     CDS             complement(5713..6366)
                     /locus_tag="IVW65_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7901654.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            6498..7340
                     /locus_tag="IVW65_002841"
     CDS             6498..7340
                     /locus_tag="IVW65_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7901655.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            7498..8394
                     /locus_tag="IVW65_002842"
     CDS             7498..8394
                     /locus_tag="IVW65_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7901656.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8397..9110
                     /locus_tag="IVW65_002843"
     CDS             8397..9110
                     /locus_tag="IVW65_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7901657.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9130..9648
                     /gene="msrA"
                     /locus_tag="IVW65_002844"
     CDS             9130..9648
                     /gene="msrA"
                     /locus_tag="IVW65_002844"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7901658.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            9645..9872
                     /locus_tag="IVW65_002845"
     CDS             9645..9872
                     /locus_tag="IVW65_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7901659.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10024..10575
                     /gene="lepB"
                     /locus_tag="IVW65_002846"
     CDS             10024..10575
                     /gene="lepB"
                     /locus_tag="IVW65_002846"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7901660.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(10658..11200)
                     /locus_tag="IVW65_002847"
     CDS             complement(10658..11200)
                     /locus_tag="IVW65_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7901661.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11437..11613)
                     /locus_tag="IVW65_002848"
     CDS             complement(11437..11613)
                     /locus_tag="IVW65_002848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901662.1"
                     /translation="MISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEMFSLEVQ
                     DLDGNTLEPVWMKM"
     gene            complement(11610..11834)
                     /locus_tag="IVW65_002849"
     CDS             complement(11610..11834)
                     /locus_tag="IVW65_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901663.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKQVVL"
BASE COUNT         4210 a         2069 c         2416 g         3390 t
ORIGIN      
        1 aaaataaagg ggatggacga tggaatttgc tgagttagtt attgtatttg cagtaacgat
       61 caccttatcg aacatagcta gccgcatttt gccaatgatt ccagctccac tgatccaaat
      121 tttccttgga gtggtattag gtctgacaga gtggggacaa tcaatcgatt ttgaacctga
      181 attgttctta gttatgatta ttgcaccatt gttatttaga gaaggagaaa aagcagatat
      241 ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg gcatttggag gagtcatttt
      301 gacccttgtc ggggtaggtg cgacgttatc attcctgttg ccaagtgtcc cacttgctgc
      361 ctgctttgct tttggagcag cgttaggtcc gacagatgcg gtagcagtca gttctctttc
      421 tggacgagtg aatatcccta aaaaggcgat gcatattttg gaaggagaag gtcttttgaa
      481 tgatgcatct ggtgttactg ccttccagtt tgctcttggt gcgctgatca ccggatcttt
      541 ttcagcagta aacgcgggga tgagtctagt agtttctagt attgggggag ctttgatcgg
      601 ctttttgctt gtctggttca aacaaaaaat aattcatttg atcgaaaaag catcagcaca
      661 agatgtcaca gggtatctgc tgatcgaact tttgcttcct tttctagcct atgtattggc
      721 agaggcattt gatgtatctg gaatcattgc tgcagtggca gcaggtattc tccaagcttc
      781 tggttttagg aaaatctctg tgtttgatgc agaattgtcg agcttgtccc acagtacatg
      841 gacaactatt gcttttacac tgaatgcatt agtatttata tttttaggaa tcgaattgac
      901 gcaagtgttc tcgccagtat ggggagacgg tttatatcca aacggtcttt tattagcgat
      961 tatcgtatta atctctgtca tgctttttgt gatcagattc atttctatca gtctttttta
     1021 tgtattcaaa gatggcagta aaaagttcaa aaaacagttg aacgagatct tgatcttaac
     1081 atttggcggt gtaaagggaa cagtcagttt ggctacgatt tttattttgc cattttcaat
     1141 caataatatg atgttttacc agcggtcttt gcttttgttt ctgacggcgg gtgtcatctt
     1201 agtgactttg gtcataggga tcattgtctt gcctatgctg acggaaacag aggaagcgaa
     1261 aagcacggat ttgaatgctt tgatgatttt agaagaggtc gttgaagttt tgcgaaaaga
     1321 aataaaggaa attgatcgta atacaaaaga atttttagcc actgaagcag tgatagaaaa
     1381 ttatcaagaa cggatacgtg atctgtactt ggaagattta accgatgatg aaaagcaaga
     1441 agtacaagaa atacaagcac tgattttgtc gatcgaacgg gatggattgg atgaaagtta
     1501 tcgttcgggc aagctttctt ctaatggtta tcgtttttac tcccgttttc tttctcgatt
     1561 cgaacattct atcacaagcc agattttgtc ttttatcggc ttttggttca ttgttgtccg
     1621 taggttaatg cgaatcgttc tccatccgaa aatgttttgg caaagaagat atgctgatcg
     1681 tcagacattt attagtgatg aagatatcca agaaatcaga gagacatatc aaaaaaatac
     1741 tcagttgatt attgaaagtt tagataactt gactgatatt tatgatgaaa cgatggttca
     1801 attttttatc cagcaacgca aacttgaagg aataaaaatg aactcgggga atttgatttc
     1861 ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa atgctaagag gttattatct
     1921 tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa atcacgacgt tttctgcgaa
     1981 cagctatcgc aggaatatca atttgttaga atcttatacg atgaacaaac catctgataa
     2041 cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa acgagtccaa taaaataaaa
     2101 aggtaaaaat tttcaagttt tataaaaaac aaaacatatc agaacaggat cgaatcggtc
     2161 ctgttctttt tttgagaaca caaaaagaac aaataccaaa aaaattttcc tgaagttcac
     2221 tatttttatg atacaatatc caagataagg agggaacatg ttgaaagaat taaaagtaac
     2281 tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac cagatttctt ttttggtcca
     2341 cgaaaaagac cgtatcgggt tgattgggac gaacgggacg ggtaaaacaa gtctcttaaa
     2401 tattattgcc ggtatcgata gcagcgacgg tgatcggcaa acagtgtttt atccaactga
     2461 ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc gatgaattaa ctgttctcca
     2521 agcagtcttt caaggaaaca gccccttgat ccaaactgtt cgagcatatg aggaagcatt
     2581 gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa aaacgttatg ctaaagcaga
     2641 agaacaaatg aataaagaag atgcttggac tacagatacc aacgcaaaga ttattttgca
     2701 aaaattagga atcagtgaat tagataaaaa aatcagtact ttatcaggtg gtcagaaaaa
     2761 acgagttagt ctggcgcaag tgttgattga tgaacccgat ttactattac tagacgaacc
     2821 aacaaaccat ttagattatg aagcgatcga gtggttggaa aactatttga aacaataccg
     2881 tggcgcacta ttgatggtta cccacgatcg ttattttcta gatagagtag caaaccgaat
     2941 cttcgaactt tcatttggaa aattatacga gtataaagga aattatgaag cttacgtctt
     3001 agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa gaaaagcgca agcggcttta
     3061 taaacaggaa ttagcttgga tgagggctgg cgtacaggca cgtggcacca agcagcaagc
     3121 acggatcaac cgtttcgaag atctaaagga gaacttgtac caagtaaacc aagaagatga
     3181 tttagagctg aacctagcga cacaacgttt agggaaaaaa gtactggaaa tcaaagatgg
     3241 cagttaccgt ataaatgacc aaacactgct agaacattta gatttattga ttcaatctcg
     3301 ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa tcgacattgc ttaatattct
     3361 agctggcaga attcccctgg atagcggtac aatgtctatc ggtgaaacgg ttcatcttgc
     3421 gtattataca caagaaaatg aagaaatggc accagataaa cggatgattg cttacttaca
     3481 agaagctgct gaagaagcaa aaacagcaga tggcagtcaa atcggagtag cagaattact
     3541 tgaacgattt ttatttccaa gatttatgca tgggacactg ataggcaaac tttcaggcgg
     3601 agaaaaacgc cggttatttt tgttgaaact gctgattcag caacctaatg ttttattatt
     3661 ggatgaaccg accaatgatt tggacatagc aacattgacg attttggaag attattttag
     3721 aagttttcca ggtgctgtga tcactgtttc ccatgaccgt tatttcttgg acaaggtagc
     3781 agataagtta ttagtattcc aaggaaacgg aaaacaagaa ctttattacg gaaacatgtc
     3841 cagttattta ctgaagcaaa aagagaccca gcagcctgct gaaaaagcga aaccaaaaat
     3901 acagagcaag gaacctgctg aaaagaagaa attatcttac atggaacaaa aagaatggga
     3961 aacgattgaa gatgagattg cagaacttga agaaaaaatc tcattgctcc aagaagagat
     4021 gaatcatcaa ggagataatt tcactcgatt acaagaattg caaaacgacg tatctgaaac
     4081 agaagcacaa cttgaagaaa aaatggcacg gtgggaatac ttgagtgaat gggtagaaga
     4141 ttaggaggaa aaaggatgga acaagcgtat cttgacttag gaagaaaatt gttggaagaa
     4201 gggcatcgta aagaagacag aacaggcaca ggcacattaa gcttattcgg ataccaaatg
     4261 cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga aacgagtacc ttttggattg
     4321 atcaaaagcg aattgctttg gtttttaaaa ggagatacga atatccgcta tctattacaa
     4381 aataaaaacc atatttggga tgaatgggca tttgaacgct acatcaaaag tccagactat
     4441 aatggaccag atatgactga ttttggccat agatgtttga cagatcctga attcaatgaa
     4501 gaatataaaa agcagagcaa gcttttttgc gagaagatcc taaccgatga ttcctttgct
     4561 gaaacatacg gcgatcttgg acacatctac ggctatcaat ggcgtcactg ggaaacaaaa
     4621 gacggtggct ttatcgatca aatcaaacaa gtgatcgaag caatcaaaaa gactcctgat
     4681 tctagaagat tgatcgtttc tgcatggaat ccggaagatg ttcctagcat ggctttgcct
     4741 ccttgtcata cgatgtttca attttatgtt caagagggac ggctaagctg ccaattgtat
     4801 caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca ttgcaagtta tgcgttgctg
     4861 actcacctaa ttgcacatga aacaggttta gcggttggcg aatttgttca cacattagga
     4921 gatgctcatc tttatatcaa tcatatcgaa caaatgaaag aacaattgag ccgagaaata
     4981 cgcgcatttc cgactttagt tttgaatcag gaaaagcagt cagtatttga ttttgagatg
     5041 gaagatatcc aattagaagg atatgaccca catccggcga tcaaagctcc tatcgctgtt
     5101 tagaatcaga aaggatgaga aaatgttagc tgcaatttgg gcacaggatg aaaacggatt
     5161 gattggaaaa gaagaccgtc ttccttggcg tttgccaaat gatctgaaat tcttcaaaca
     5221 aatgacagaa gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg
     5281 tccgttgccg aatcgtcaga cgattgtttt gacgagagat tcttcctata aagcagaagg
     5341 cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa cagacggtat
     5401 ctttttcatc gctggagggt cagctgttta tcaagaattt ctgccatttt gtacgatatt
     5461 ataccgtaca gtcatccatc atgtattcga tggagatgct tattttccac ctgttgactg
     5521 gtctgattgg tcactgatca atactagtca aggagaaatg gatgaaaaaa atcgttatcc
     5581 gcatcaattt gaaacgtatc agcgtaatga gaatcctgta aaaagcggga caggcgaatt
     5641 gacgatataa aaaagctatg gatagactag ctgttattta gagctgtccc atccatagct
     5701 ttattttttt tattaagtaa agaataaaac agagaagtac ataaaacctg cacctaacat
     5761 aacaaacaaa tgccaaacaa catgcatata gcggatagaa ttcaagctat aaaagaatgc
     5821 gcctatcgtg taagaaagcc cgcctgctac catcaaaata acaccagtca ttccaagaga
     5881 gtgataaaga ggaacagcag ccacgacaca gagccacccc ataaaaatat agatgatcgt
     5941 agatactttt gatactctct cccgtttatg caacgtcaac gctttatata taatgccaag
     6001 aatcgcaaac aaccagatta atacaaataa agtccaacct agccatcctc ggatactcag
     6061 caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa atcgaactat gatcgaatac
     6121 ttggaacacc ttttttgctt ttgtaaaaat caatgaatga aacaaagtag acgttaaaaa
     6181 taaaagaatc atcatcgatc catagatagc ataagagaca atatggaggg aggaatgcaa
     6241 gtgggcacct tttactaaaa gaaccactag tcccgcgata ctcaatccag tcccaattcc
     6301 atgggtgact gcattaaaca cttcatttaa tatttcatac gttcgattaa atttcgtttt
     6361 ttccatgaaa acggctcctt tactaggtgt aattaatgaa aaatcattat ttgtctgtta
     6421 tactaaaaat agaaattgtc ttacatgtag tttaacatga ataaggcatc ttgttaaaga
     6481 aagagagaga gaaaataatg acaaatgtaa aaatcgtaac agattcttca tgtacgatgg
     6541 aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc gctatccatc atgatcgacg
     6601 gaacagtata tcccgatgat gaccaactag acggcgaaag atttatggaa atgatggcga
     6661 aagcaaaaaa tctgcccaaa acaagccaac caccaattgg agaattcatc tcgatgtatg
     6721 atgagctagg agaagacggc agtgaagtga tttcgatcca tatgacaaaa ggcttgagtg
     6781 gaacagtaga agcagcaaga caagccagta atctaacaaa aacgaaagta acggttatcg
     6841 acagtgatac cactgaccaa ggattatctt ttcaggtaat tcgagcagct gaattagcaa
     6901 aagaaggtaa aacagtagag aaaatccttg aagaagtcaa aaaagtacaa gacaatacaa
     6961 aattatacat cggaatatcc acactagata acttggtaaa aggtggaaga atcagccgaa
     7021 caactggttt gctgtctagt gttttgaaca tacgcgtggt gatgaatttc gcccattctg
     7081 aattaatccc tgtaactaag gggcgaggaa agaaaacgtt cgataaatgg tttaataatt
     7141 tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat cggtatttca catgcggatg
     7201 caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga attattcccg gacatgcata
     7261 tccctgtact gcatacgaat ccggttatcg ctacgcatac tggtaaagac gcgtttgcaa
     7321 tcatgtatta tgtagattag tcgaaaagag gataagccct cagcctgaaa ggcttgcagc
     7381 ttattctctt tttctttata aaattaggaa aataattgtt ttcttttttc ataaagacta
     7441 cctttttgat aaaatttttg ataacattag aaccaatcga atacaagagg tgaaaaaatg
     7501 aaattaacga aacgacattt gatcctcgtt ttgttcccat tattaattgc gatcatcatc
     7561 tatgcaggtt tagctgcttc tataccgaaa gctggcccac taatgaaacc agtaactact
     7621 gtgagagaag atccaaataa aaaagaaaaa cttcactaca tagctatcgg agattcgctg
     7681 actgaaggag taggagatca aaccaagcaa ggaggattcg ttccccttgt cgcaaatgat
     7741 ctaaaagacc gttataattt gaccgcggtt gaaacagaaa actatggtgt aaatggtgaa
     7801 cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag aaattcgaaa caatatcaaa
     7861 acagctgatt ttatcacact gactgttggc ggtaatgatt tgatgaaagt tatccaaaat
     7921 aatttattcg ggttaacggt caattccttt aaaaagccac tgaaaaacta tcaggaaaat
     7981 gtcacagaac tattggaaga atttcgatcc ctcaataaaa ccgcacctat ttacatttta
     8041 ggcatctaca atccatttta tttgaacttt cctgagatca ctgacatgca gacaattgtt
     8101 aacaattgga acaaaggaac agaaacggtg acaaaaaaaa tgccgaactg ctactttatc
     8161 ccaatcaatg atttgcttta tcaaggattg aatcaggaag taggtgtcac agaagaagga
     8221 acagatcaaa caactagcac gacagaaaat ctaaatggca tcaaaaataa tgtgctctat
     8281 gaagaagatc atttccatcc aaataattta ggttatcaat tgatggcaaa tgcagtgaga
     8341 gatcagctga tcgagacaca aaaccaatgg ttagagaagg aggatgatca ataaacatga
     8401 aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac aaattactgg aaattcgcct
     8461 tccttatcct tcttggggta gtacttggtt cgaccgtatt tttaggcagc cgtatttttg
     8521 ctaatcggga accagaactc ccagaaatac cagctcttac ggaaagacaa ggagatcctg
     8581 tcttaacgat caattcaaat aaagaaaaag tcaatcaaat catcagcttt ttccttagtg
     8641 agtatcaaaa agatagtgat attgaatata aattttattt ggaaaatgaa gcactactca
     8701 atggtacttt cgaagtacta ggttttccta tcgatttcta tttatatttt gatccgtatg
     8761 tgatggaaaa cggaaatgtc caactaaaag ctagaagtct ttctatcgga acattgagtt
     8821 tgccaatcaa agatgtgatg aatatgatca agagaaacta taaattacca gattggatcg
     8881 aagtcaatac agatgatctg actatcatgc ttaggttgga tcaattccga atgcaaaacg
     8941 ggatgtatat taaagcagat aaaatcgact tagtaaatga tgacattcgg tttagtctat
     9001 atttgccaaa agatacctct gatgcttcaa acaactcaaa taccaacaaa actagcacca
     9061 gcagtactga aagcaagaca aaagaaaata aaaaatcatc ggatgagtag tattagaggg
     9121 ggaatcataa tggaacgagc aatatttgca ggaggctgct tctggtgtat gatccagcct
     9181 ttcgacacgc ttcctggtat ccatacagtg atgtcaggat atactggagg gcatgtacca
     9241 gatccgactt atgaacaagt aaaaagccaa acgactggac atacagaagc agtggagatt
     9301 atctatgatc caacattgat ttcatatgaa caattagtag aactttactg gcagcaaact
     9361 gatccaactg acgctttcgg acaattcgag gatcgcggtg acaattatcg cccggtgatc
     9421 ttttatttga cagaagaaca gcggatcatt gctgaaaaaa gtaagaaaca gttagctgaa
     9481 agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac cagcccaacc attttatcta
     9541 gcagaagaag accatcaaga gtattataaa aagcatcctg aaaattttaa aagaaaccat
     9601 gcacgccggg ctgcctttat cgaagaaaat tggaggaacg aagggtgaat cgaagctttt
     9661 ttcattattt aatgacactg cgaggcggtg gcacacatga tgctttatct gttttcgcta
     9721 cagaagccgg taaagacatc caatttccta agcacagctc tagctacgaa gaaatttcta
     9781 attatctgga actgaatgtc gactatttgc cgtccatgga tatctttgac gaagcatggg
     9841 aaaaatattt agaaaataat catgacatat aagagatagt tttgtacttt atgatcaaaa
     9901 aagttttttg tccaatagcc taattgaaag aattattttt atttcagcta tctagtcgca
     9961 tttttttgat atttttctta taataaagaa agaatgaaaa aaagagggat ggattgatag
    10021 agattgacaa aaaaacaacg ctatattaat cgcttctggc ttgtattcaa atacttactt
    10081 gtttctgtat tccttgcgtt catgttgcgg ggatttttat tcatcccggt accagtggaa
    10141 ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg ttgtgatgga aaaattttcg
    10201 gagatcagac gatttgatat tgttgttttt caattagcag atggcacgat ttatatcaaa
    10261 cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa atgatcaatt gaagatcaat
    10321 gggaaagtag ttaaggaacc ttatctaact aaaaacctga aatcagatca tgcaaatgca
    10381 tcctatacga cggactttac actacaagag ctaacgggac aatccaagct tccggaagat
    10441 aattattttg tacttggaga taatcgaaga gtttcaaaag atagtcgttc gtttggcacg
    10501 atcaataaaa ctgatattct aggtaaagca agatttgtct attatccact tgatgaaata
    10561 aaatggatcc agtgagaaac aataaagcgt gcagtctgtt gagactgcac gctttattgt
    10621 ttaatccaaa atcagtttac taccttagta cgtaaaatca aacagctcgt ttaaacactt
    10681 ttctagcctc taaagttttg ataaatgaat aaacaaccat tatttgaata gggatttgga
    10741 tcatattcct gatgaatcta cctagaaaaa ttactttgaa atctgcacca aacaaaacag
    10801 acatcagcca agaattgata aaaaggctga ctgggatcat aatcaataat tgggcagcga
    10861 tgacggaagg caggtttatc tttttatgat ataaaaataa gccattaata aatccaccaa
    10921 ggaaagccgt gataagaaaa gcagcttgaa acgttcctga accaaatatc gtgaaattta
    10981 aagtgttcga caacatttca agcaaaccag cccagatcgg tccatataga taacccataa
    11041 gcgccatact taaaaatccg aatgtgattt gaaaataagc aggaccaatc gctatcttgt
    11101 ataagatgac atcaagggct aataaaagag cagaggtcga caacaatctt gcattcattt
    11161 cgggcaatct aaaaacgtct tttgcaatca aagtgttcaa aatattctct cctttgtcag
    11221 ttaataataa aaggatacca taaaatcacc aatgtgtcat gacacattgg tgatttacat
    11281 acactggcat ctaacaatta aaaaaagggt gagaagactg cttttataaa ctgtaaaaaa
    11341 gagattgtac gaataaaaaa aatagaggcg gagaatactt atcgtatcat ccgcctttgt
    11401 ttttttgtta taaaaaacgt cagattagtt atctgcttac atcttcatcc atacgggttc
    11461 taatgtattt ccgtctagat cttggacttc taatgaaaac atttcttctt caggcattcc
    11521 catatctact tcgtggaaag taccaccatt ttcttttgca atttgagcaa atttttttac
    11581 ttcatccgca ttttcaacac taaaagaaat caaagcacca cttgtttttt attatcagct
    11641 atttctttat cttttataaa ttttttataa aaatcatgag agagaagcat tatccaaaaa
    11701 gtgtcatccc ataccattga accagcttct tcagtagaaa attcttcatt ttttttaaaa
    11761 cccagctttt cataaaaagc aacggaacga ttgacatcag caacaggaaa attaacaaaa
    11821 accatactat tcatttgttt cgtctccttt ctgttttgtt cgtctatcat ctcttattta
    11881 ggaaacgctg tcaaaaattt agattcatat atagaaacca aaataaaaat ttgcatcatc
    11941 aaaaaagtga agatgtgata gaatgaggac tggttcactt tcacttaact aatagatgga
    12001 atttctttat aataaaaaag tgaatttata ttgaaaaaga aaaaaacaag taaagccggg
    12061 gaatattccg gtatgaaagg aatgg
//
LOCUS       DACCWK010000304        11426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.082, whole genome shotgun sequence.
ACCESSION   DACCWK010000304 DACCWK010000000
VERSION     DACCWK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.082"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            39..977
                     /locus_tag="IVW65_002850"
                     /pseudo
     CDS             39..977
                     /locus_tag="IVW65_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IVW65_002851"
     CDS             980..3163
                     /locus_tag="IVW65_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7901664.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IVW65_002852"
     CDS             3160..4161
                     /locus_tag="IVW65_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7901665.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4158..5090
                     /locus_tag="IVW65_002853"
     CDS             4158..5090
                     /locus_tag="IVW65_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7901666.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            5335..5451
                     /locus_tag="IVW65_002854"
     CDS             5335..5451
                     /locus_tag="IVW65_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7901667.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IVW65_002855"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IVW65_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7901668.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IVW65_002856"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IVW65_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7901669.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IVW65_002857"
     CDS             9421..10683
                     /locus_tag="IVW65_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP7901670.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IVW65_002858"
     CDS             10928..11410
                     /locus_tag="IVW65_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP7901671.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3771 a         1897 c         2395 g         3363 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaatg agaaaggaag atttaatgat gaaatttaga aaaaatcaga
     4201 ataaagaaaa acagatacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcata
     4261 aaaaggttgt gattgccttg tgggtacttt tagggcttag tttcagcttt gcgatattca
     4321 agcactttac agctatagat actcatacta ttcacgaaac aactatcata gaaaaggaat
     4381 acgttgatac tcatcatgta gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggagcaatc cgataagtcc attgataatc gaatggaaag tctaaaaggc tatctgacag
     4501 atgaacttca agctctcaat gttgatacag tacgcaaaga tattcctgta tcgtcttctg
     4561 taagaggatt tcagatatgg acggtagagc caactggcga caatgagttt aatgtaacct
     4621 acagtgtaga ccagctcatt acagagggag aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagat ggttctggaa atatggtact ggttaagaat ccgaccatta
     4741 ccaacatacc taagaaatca agttataaac caaaagccat tgaaagtgag gggacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tgtaaaataa caaatattgg
     5101 tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat acatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aaatcc
//
LOCUS       DACCWK010000305         8296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.110, whole genome shotgun sequence.
ACCESSION   DACCWK010000305 DACCWK010000000
VERSION     DACCWK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.110"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            60..707
                     /locus_tag="IVW65_002859"
     CDS             60..707
                     /locus_tag="IVW65_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HAP7901672.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1400..2338)
                     /locus_tag="IVW65_002860"
                     /pseudo
     CDS             complement(1400..2338)
                     /locus_tag="IVW65_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2338..2955)
                     /locus_tag="IVW65_002861"
     CDS             complement(2338..2955)
                     /locus_tag="IVW65_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7901673.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(3487..4977)
                     /locus_tag="IVW65_002862"
     CDS             complement(3487..4977)
                     /locus_tag="IVW65_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7901674.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(5020..5319)
                     /locus_tag="IVW65_002863"
     CDS             complement(5020..5319)
                     /locus_tag="IVW65_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901675.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5685..5969)
                     /locus_tag="IVW65_002864"
     CDS             complement(5685..5969)
                     /locus_tag="IVW65_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7901676.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6488..6973)
                     /gene="vanZ"
                     /locus_tag="IVW65_002865"
     CDS             complement(6488..6973)
                     /gene="vanZ"
                     /locus_tag="IVW65_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP7901677.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(7126..8037)
                     /locus_tag="IVW65_002866"
     CDS             complement(7126..8037)
                     /locus_tag="IVW65_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAP7901678.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2524 a         1479 c         1245 g         3048 t
ORIGIN      
        1 acctatagag gatgggcgac ttctgcctaa ttctgttaaa atttaggagg aagttatata
       61 tgacttttaa tattattgaa ttagaaaatt gggatagaaa agaatatttt gaacactatt
      121 ttaatcagca aactacttat agcattacta aagaaattga tattactttg tttaaagata
      181 tgataaaaaa gaaaggatat gaaatttatc cctctttaat ttatgcaatt atggaagttg
      241 taaataaaaa taaagtgttt agaacaggaa ttaatagtga gaataaatta ggttattggg
      301 ataagttaaa tcctttgtat acagttttta ataagcaaac tgaaaaattt actaacattt
      361 ggactgaatc tgataaaaac ttcatttctt tttataataa ttataaaaat gacttgcttg
      421 aatataaaga taaagaagaa atgtttccta aaaaaccgat acctgaaaac accataccga
      481 tttcaatgat tccttggatt gattttagtt catttaattt aaatattggt aacaatagca
      541 gctttttatt gcctattatt acgataggta aattttatag tgagaataat aaaatttata
      601 taccagttgc tctgcaactt catcattctg tatgtgatgg ttaccatgct tcactattta
      661 tgaatgaatt tcaagatata attcataggg tagatgattg gatttagttt ttagattttg
      721 aaagtgaatt taattttata cacgtaagtg atcataaaat ttatgaacgt ataacaacca
      781 cattttttgg ttgcttgtgg ttttgatttt gaatttggtt ttgaacttat ggactgattt
      841 attcagtcca ttttttgtgc ttgcacaaaa actagcctcg cagagcacac gcattaatga
      901 cttatgaaac gtagtaaata agtctagtgt gttatacttt acttggaaga tgcaccgaat
      961 aaaaaatatt gaagaacaac tagcaaaaga ttttaaagag ttattttatt ttaagtcttt
     1021 ataacatgag tgaagcgaat ttttaaattt cgatagaaat ttttacatca aaaagccccc
     1081 tgtcaaaatt gacgaagggg gttttttggc gcacgctttt cgttagaaat atacaagatt
     1141 gaaaatcgtg tataagtgcg ccctttgttt tgaacttagc acgttacatc aattttttaa
     1201 aatgatgtat aagtgcgccc ttttaaattt tgagtgatta tattttttga gttagaaaaa
     1261 gggattggga aaatttccca aaataattta aaaaataagc aaaaattttc gatagagaat
     1321 gtgctatttt ttgtcaaagg tgtatacctt gactgtgctt gctgttacat taagtttatt
     1381 tttaagttat taaaaaagaa atagctttta aagtttggct cgctgtcgct ttataaagct
     1441 gattgacttt tgattgcaaa ctacttaaag aaaacaaact cggactattc gttttcttct
     1501 ctttggtttg aacatcagca attatcccct cttgattgcc tattttagct tgtttagaag
     1561 aaacaaaagc taaaagctcc tcttgggttt taaaacgctg tgtggggctt agaacgccct
     1621 taaacgaccc ttggtttact tttatactag cttccacctc gaaaaaaggt tcttttttaa
     1681 aattctctat ggcttcctgg cgctgaaaaa taaggtataa ggtgggcgtt tgaacacgtc
     1741 ctagtgaaaa tgtaccttgt acgcccttct gttgtaaatt taacgtatac aaagggcttg
     1801 cgttcatgcc gatcaaccaa tcggcaattt ggcgtgtttg cgcttcttga taaaagggat
     1861 agtaattcat tccaggttgc aaattttgaa aaccgcttcg gattacatct ttttctaagc
     1921 tattgatcca tagtctttta aatgttttat cttttgaaaa ggcatttgct ttatggataa
     1981 tcgaccaggc gatattttca ccttctctgt cgctatctgt tgcaacaata attgtatttg
     2041 cctttttgag aagttctgca acaattttaa actgctttcc cttatctttt gcaacttcaa
     2101 aatcgtatcg atcaggaaaa atcggcaaag attcaagttt ccaattttgc cacttttcgt
     2161 cataatgacc tggttctgct aattccacta aatgcccaaa accaaaggtg ataaacgttt
     2221 catctgtaaa tagtgggtct ttgatctcaa aataaccgtc ttttttggtg ctttgtttta
     2281 aagcacttgc gtaggctaat gcctggcttg gtttttcagc taaaataacc gtactcatta
     2341 actatccctc ttttcattgt tttttctttg atcgactgtc acgttatatc ttgctcgata
     2401 ccttctaaac gttcggcgat tgattccagt ttgttcttca acttctttat cggataaacc
     2461 attcaaaaac aaatcgaaag catgctttaa acgtggatca ttttctttaa atttatgctg
     2521 acgacctttg aatttgcctt ttttcttagc aatttcgatt ccttgtgcct gacgttcctt
     2581 aatttttttt cgttctgatt ctgcttgata cttgtacaat tcaatgacaa ggctattaat
     2641 caaacgcctt aaattttcat cttcaatacc attcattgag ggtaaattta agacttccag
     2701 ggttgccccc ttaatttgaa tttgattcat caattctgtt aattctttat tatttcgtcc
     2761 taatcgatct aattcagtaa caataacaat atccccttca cgaatatagt taagcatagc
     2821 ttgtaattgt gggcgttcga ccgattgacc gcttaatttg tctgaaaaga ccttagaaac
     2881 gccctgtaac gcttgtaatt gccgatctaa gttctgttct ttgctactga cacgtgcata
     2941 accaatttta gccattttca accaacctct aaaattctct cggttgcaat aaccaatcag
     3001 caatatctac tttttcaatt tcaaattgct tatcagaaat tgtcttttcg taagcgataa
     3061 aatcttgcgc atattgttgc tcattaaaaa tagccaccac ttcgtcattt tctaaaactc
     3121 gataaataaa ttttttcatt ttactcctcc tattatgccc aacttaaatg acctattcac
     3181 caagtcaatt atactgctaa aatcatatta ggacaaatag gtatactcta atgacctata
     3241 aaagatagaa aattaaaaaa tcaagtgttc gcttcgctct cactgcccct cgacgtttta
     3301 gtagcctttc cctcacttcg ttcagtccaa gccaactaaa agttttcggg ctactctctc
     3361 cttctccccc taataattaa ttaaaatctt actctgtata tttctgctaa tcattcgcta
     3421 aacagcaaag aaaaaacaaa cacgtatcat agatataaat gtaatggcat agtgcgggtt
     3481 ttattttcag cctgtatcat agctaaacaa atcgagttgt gtgtccgttt tagggcgttc
     3541 tgctagcttg tttaaagtct cttgaatgaa tgtatgctct aagtcaaaag aatttgtcag
     3601 cgcctttata tagctttctt tttcttcttt ttttacttta atgatcgata gcaacaatga
     3661 tttaacacta gcaagttgaa tgccaccatt tcttcctggt ttaatcttaa agaaaatttc
     3721 ctgattcgcc ttcagtacct tcagcaattt atctaatgtc cgttcaggaa tgcctagcac
     3781 ttctctaatc tcttttttgg tcgtcactaa ataaggcttg tatacatcgc ttttttcgct
     3841 aatataagcc attaaatctt ctttccattc tgacaaatga acacgttgac gttcgcttct
     3901 ttttttcttg aatttaaacc acccttgacg gacaaataaa tctttactgg ttaaatcact
     3961 tgatacccaa gctttgcaaa gaatggtaat gtattcccta ttagcccctt gatagttttc
     4021 tgaataggca cttctaacaa ttttgattac ttctttttct tctaagggtt gatctaatcg
     4081 attattaaac tcaaacatat tatattcgca cgtttcgatt gaatagcctg aactaaagta
     4141 ggctaaagag agggtaaaca tgacgttatt acgccctatt aaaccctttt ctcctgaaaa
     4201 tttcgtttcg tgcaataaga gattaaacca gggttcatct acttgttttt tgccttctgt
     4261 accgcttaaa accgttagac ttgaacgagt aaagccctta ttatctgttt gtttgaaaga
     4321 ccaatcttgc cattctttga aagaataacg gtaattagga tcaaaaaatt ctacattgtc
     4381 cgttcttggt atgcgagcaa taccaaaatg attacacgtt agatcaactg gcaaagactt
     4441 tccaaaatat tctcggatat tttgcgaaat tattttggct gctttgacag atttaaattc
     4501 tgattttgaa gtcacataga ctggcgtttc taaaacaaaa tatgcttgat aacctttatc
     4561 agatttgata atcatagtag gcataaaacc taaatcaata gcggttgtta aaatatcgct
     4621 tgctgaaata gtttcttttg ccgtgtgaat atcaaaatca ataaagaagg tattgatttg
     4681 tcttaaattg ttttcagaat gtcctttcgt gtatgaacgg ttttcgtctg catacgttcc
     4741 ataacgataa acgttgggtg tccaatgtgt aaatgtatct tgattttctt gaatcgcttc
     4801 ctcggaagtc agaacaacac cacgaccgcc aatcatgctt gattttgagc gatacgcaaa
     4861 aatagcccct ttgcttttac ctggcttggt agtgattgag cgaattttac tatttttaaa
     4921 tttgtacttt aacaagccgt catgaagcac agtttctaca acaaaaggga tattcattca
     4981 gctgttctcc tttcctataa atcctataaa ataggttgtt taattaactt ggtttgcttt
     5041 ttcattcaac tgtttcaata ttgcatgttt tgaaaaagat ttttttcctt tataagtcaa
     5101 tttttttcca ctaatcgaat aaattatttt gttattttct attaacttat atatataatc
     5161 ttccccctcc gaagaaaaat acttatctga ttttgtttct aagtagatat ttctcttttc
     5221 taactctttc ttaaacgttt ctagtgtata gatatttgct aattttctta tctccaataa
     5281 actatttttt atataagttt tacattcatc atgattcata caaactccac cttctataaa
     5341 tgaatacaaa aaaagcaatc aaacgatttc cgattgattg cttaacaatt cttaaattca
     5401 gtagcttaga tacttgaaaa ctctctgatt tccctatata atgatagtac ggttatatac
     5461 cgtcttcaaa caaagttaat taaataactt cttacgaggg aagagttcat ctgactaact
     5521 gataagcgtt ggtttggcaa tcttatcggg ctatgcattt ataaaatgtc gtcaaacatt
     5581 ttataaatgt gtcatggctc ttttttcgtt tctattcagt tcgttgtttc gttatatcta
     5641 gtataccgct tttaaaaaaa ataagcaacg atttcgtgca ttattcacac gaagtcattg
     5701 cttttttctt cttccatttc taaatccaat gttacttgtt ctgattctgt ttctggttct
     5761 ggttctgttg gctcatttgg gattaaatcc actactagcg ttgagttagt taactttgca
     5821 atttgttcta gtgtttttat ggttggatct gattttcctg attctattcg tgaataattt
     5881 gatctactca tttctaattc ttgggagaat ttttcttttg atttggtacc tctcatcttt
     5941 tttaagcttt ctctaaatgc tagttccaag tttctccccc ctctttttgt gtaataaaat
     6001 tcacgcttta tttaaattat agcttttaat atcttaataa acaagtgatt aatcacttgt
     6061 gatttgtgat tattccaaaa gtaggtaatt tcaccaacct ttttcaaaag tttttatcga
     6121 cgattattgg actggatcgt gtttcctaaa gcgattctaa gcgcatgttt aattttaaag
     6181 tgggtaattc tctttaaaac gtcctacaaa gattcaaaaa aagcatggct gctagtgggg
     6241 gtaccgtcag gaaaatgcgg atttacaacg ttaaggaaat ttattaaaga gaccaattcc
     6301 tcagcactaa aattgataat tgggctttct ttgttactat agtgttaggt aagcttttta
     6361 ctttatcacc ggttttcccc tcacttcaca cctacgggcg agtttcccca catcccgttt
     6421 tccatctaat ccaattcatt caatatcttt atgtgtatga aagatcagat tgcttgattt
     6481 agacatctta cacgtaattt attcttaaat gggtacggta aacgagcaat aatacgagca
     6541 aaagtatacc tacaaaaata ataactctgt ctaatttttt ttgattcata tgcttattgc
     6601 ttaaaccata taatttcagt ccaagaaagc ctccaacagt atttgtaatt acatctgtta
     6661 tgtctgtcgc tccaatagcg aagataaatt gaattatttc aaaagtaaga cttaaaacca
     6721 gtacaaaagc aaacttaggt aaaaatccga tttctttaaa attgacattc aaaagcaagc
     6781 caaatggaat aaagattata acattatcta tcatctctct gaaattccca gtagcagtaa
     6841 atggagtcaa gttaagactt ctttgatgat aattaaatac tgataaaata ttgtattgta
     6901 atttgaataa cactaaccag attagtgtca ctaaatataa agctagcaat cctctagata
     6961 atatttttcc caatataatt tcctctaatt cttttgttta agttgtatca ttagtatagc
     7021 aatgtcctag ttaacgtggg ttttaaatta aaaagctaac gttaccccaa aagtgctagt
     7081 acaataatgc tgttcattaa tttcgttggt ttcattccgc catccttacc tccttgaatt
     7141 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
     7201 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
     7261 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
     7321 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
     7381 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
     7441 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
     7501 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
     7561 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
     7621 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
     7681 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
     7741 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
     7801 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
     7861 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
     7921 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
     7981 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
     8041 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
     8101 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     8161 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     8221 tgaatttaac gaggctgaac tgcctgttga caatgggggt tctgttgcaa agttttaaat
     8281 ctactatcaa ataagg
//
LOCUS       DACCWK010000306         5555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.128, whole genome shotgun sequence.
ACCESSION   DACCWK010000306 DACCWK010000000
VERSION     DACCWK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.128"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            74..538
                     /locus_tag="IVW65_002867"
     CDS             74..538
                     /locus_tag="IVW65_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7901679.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            522..1151
                     /locus_tag="IVW65_002868"
     CDS             522..1151
                     /locus_tag="IVW65_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7901680.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1505..2983
                     /gene="msr"
                     /locus_tag="IVW65_002869"
     CDS             1505..2983
                     /gene="msr"
                     /locus_tag="IVW65_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7901681.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3030..3476
                     /locus_tag="IVW65_002870"
     CDS             3030..3476
                     /locus_tag="IVW65_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7901682.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3675..4190)
                     /locus_tag="IVW65_002871"
     CDS             complement(3675..4190)
                     /locus_tag="IVW65_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7901683.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4307..5509
                     /locus_tag="IVW65_002872"
     CDS             4307..5509
                     /locus_tag="IVW65_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7901684.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1733 a          927 c         1192 g         1703 t
ORIGIN      
        1 gacttttttc tttttttatt ctattattga caagtcaatc gttgacgagt caattattag
       61 aaaggaggaa attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc
      121 cgatcaaaaa atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga
      181 gatcatgatg agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt
      241 gcagattgat caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt
      301 ttcgcgtcag agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc
      361 ccagaagaaa atcaagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc
      421 ggctttcagc gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca
      481 actttgcaat ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt
      541 tttcaacaat tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc
      601 cacacgaaga aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata
      661 tgaaaccatg gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt
      721 tgatccgttt caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg
      781 atatgcaatg ttacgaatat ggcgaagaaa tctacaacca agcttacgaa tcagggaaga
      841 tgccaaaaga agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt
      901 caagagaaga aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca
      961 tgttagttgc gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc
     1021 agttggcaga ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa
     1081 taggaaaagc ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta
     1141 tgttcgaata aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg
     1201 tggtaaccta ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga
     1261 acttttgaat aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg
     1321 gagtatgtcc attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga
     1381 tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca
     1441 gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa
     1501 acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct
     1561 agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga
     1621 aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca
     1681 tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga
     1741 agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc
     1801 agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc
     1861 ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga
     1921 tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg
     1981 ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga
     2041 gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct
     2101 acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa
     2161 gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag
     2221 actggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa
     2281 tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat
     2341 gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat
     2401 gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca
     2461 gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt
     2521 attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca
     2581 agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat
     2641 gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc
     2701 tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac
     2761 gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt
     2821 tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat
     2881 tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat
     2941 aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt
     3001 tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt
     3061 ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc
     3121 atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt
     3181 tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag
     3241 taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa
     3301 tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat
     3361 tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga
     3421 tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga
     3481 acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt
     3541 ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt
     3601 tccaactgta ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag
     3661 gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat
     3721 tggagttcct gctgacaaag cataattctg cgctggatca caacataacg tccaaattct
     3781 tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc
     3841 agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa
     3901 gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc
     3961 cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc
     4021 tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag
     4081 atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa
     4141 cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta
     4201 cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata
     4261 ttgacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga
     4321 gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct
     4381 agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt
     4441 aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag
     4501 ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg
     4561 tttgaaaaaa acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg
     4621 attatctggg aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc
     4681 tttatttgtc tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa
     4741 agcaatcatg atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg
     4801 tggggtcttg ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt
     4861 ttgcggtttt atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt
     4921 aggcctttgg gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat
     4981 cgcattgtta tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat
     5041 tggctattct gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat
     5101 tgcttcgatt ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt
     5161 agcagcattt gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc
     5221 agtccttgta ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact
     5281 gttgacgacg attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta
     5341 tagttttgtc cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga
     5401 acatattggt gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg
     5461 ttgtatcatt tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg
     5521 ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DACCWK010000307         4802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.139, whole genome shotgun sequence.
ACCESSION   DACCWK010000307 DACCWK010000000
VERSION     DACCWK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.139"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            92..787
                     /gene="vanR"
                     /locus_tag="IVW65_002873"
     CDS             92..787
                     /gene="vanR"
                     /locus_tag="IVW65_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP7901685.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="IVW65_002874"
     CDS             765..1919
                     /locus_tag="IVW65_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7901686.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="IVW65_002875"
     CDS             2134..3102
                     /locus_tag="IVW65_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP7901687.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="IVW65_002876"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="IVW65_002876"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP7901688.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="IVW65_002877"
     CDS             4132..4740
                     /locus_tag="IVW65_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP7901689.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          921 c         1119 g         1280 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 cc
//
LOCUS       DACCWK010000308         2247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.185, whole genome shotgun sequence.
ACCESSION   DACCWK010000308 DACCWK010000000
VERSION     DACCWK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.185"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            73..756
                     /locus_tag="IVW65_002878"
     CDS             73..756
                     /locus_tag="IVW65_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7901690.1"
                     /translation="MSRSQKGLAGAGEWETLKKMLPDFKGKRVLDLGCGYGWHCIYAM
                     ENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDFPEESFDVILSSLAFHYV
                     ADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPVDNYYYEGK
                     RTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRRPMM
                     LIVSAKKKM"
     gene            789..1697
                     /locus_tag="IVW65_002879"
     CDS             789..1697
                     /locus_tag="IVW65_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7901691.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1694..2174
                     /locus_tag="IVW65_002880"
                     /pseudo
     CDS             1694..2174
                     /locus_tag="IVW65_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          777 a          320 c          505 g          645 t
ORIGIN      
        1 tcacatggtt ttaattaaaa gtatgaatta ttcaggtatg atgataatat attttttcaa
       61 aaatacagtc aaatgagtcg ctcgcagaaa ggactggctg gtgcgggaga atgggagact
      121 ttgaaaaaga tgctacctga ttttaagggt aagcgtgtgc ttgatttagg atgcggctat
      181 ggatggcact gtatatatgc gatggaaaac ggtgcttcct ctgtagtagg tgttgatatt
      241 tctcataaaa tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa
      301 tgctgtgcca tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg
      361 cttgcgtttc attatgtagc agactatgag aatttaataa aaaagatata taggatgctg
      421 aaggctggtg gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca
      481 caagactggt attataacga aaaaggagaa atactgcatt tcccggtgga caattattat
      541 tatgagggca aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca
      601 ctgaccacat atctaaatac actgctttca aatagtttta taataaatca gattgtggag
      661 ccacagccgc cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca
      721 atgatgctga ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga
      781 gtatgtaaat gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg
      841 atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag
      901 atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta
      961 atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg
     1021 aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata
     1081 aaattgatct taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat
     1141 taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata
     1201 tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt
     1261 ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg
     1321 atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca
     1381 tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat
     1441 ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat
     1501 gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa
     1561 ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt
     1621 ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag
     1681 agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc
     1741 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     1801 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     1861 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     1921 tttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc
     1981 ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa
     2041 cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct
     2101 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
     2161 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtgg ttctgttgca
     2221 aagttttaaa taaagaataa aatccct
//
LOCUS       DACCWK010000309         1285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.229, whole genome shotgun sequence.
ACCESSION   DACCWK010000309 DACCWK010000000
VERSION     DACCWK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.229"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..855
                     /locus_tag="IVW65_002881"
                     /note="aph(2'')-Ia"
     CDS             <1..855
                     /locus_tag="IVW65_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7901692.1"
                     /translation="YFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAK
                     EKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNL
                     LKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFM
                     ERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDS
                     EEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKE
                     IYKRTYKD"
     gene            complement(985..>1285)
                     /locus_tag="IVW65_002882"
     CDS             complement(985..>1285)
                     /locus_tag="IVW65_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7901693.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          504 a          119 c          217 g          445 t
ORIGIN      
        1 tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg
       61 gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa
      121 ggttatgcaa aagaaaaagc aatatataat tttttaaata caaatttaga aactaatgta
      181 aaaattccta atattgaata ttcgtatatt agtgatgaat tatctatact aggttataaa
      241 gaaattaaag gaactttttt aacaccagaa atttattcta ctatgtcaga agaagaacaa
      301 aatttgttaa aacgagatat tgccagtttt ttaagacaaa tgcacggttt agattataca
      361 gatattagtg aatgtactat tgataataaa caaaatgtat tagaagagta tatattgttg
      421 cgtgaaacta tttataatga tttaactgat atagaaaaag attatataga aagttttatg
      481 gaaagactaa atgcaacaac agtttttgag ggtaaaaagt gtttatgcca taatgatttt
      541 agttgtaatc atctattgtt agatggcaat aatagattaa ctggaataat tgattttgga
      601 gattctggaa ttatagatga atattgtgat tttatatact tacttgaaga tagtgaagaa
      661 gaaataggaa caaattttgg agaagatata ttaagaatgt atggaaatat agatattgag
      721 aaagcaaaag aatatcaaga tatagttgaa gaatattatc ctattgaaac tattgtttat
      781 ggaattaaaa atattaaaca ggaatttatc gaaaatggta gaaaagaaat ttataaaagg
      841 acttataaag attgattata taatatatga aaagctatta taaaagacat tagtattaaa
      901 tagtttaaaa aaatgaaaaa taataaagga agtgagtcaa ttccagactc ctgtgtaaaa
      961 tgctatacaa tgtttttacc atttctactt atcaaaattg atgtattttc ttgaagaata
     1021 aatccattca tcatgtaggt ccataagaac ggctccaatt aagcgattgg ctgatgtttg
     1081 attggggaag atgcgaataa tcttttctct tctgcgtact tcttgattca gtcgttcaat
     1141 tagattggta ctctttagtc gattgtggga atttccttgt acggtatatt gaaaggcgtc
     1201 ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat tttggttggt tctgttgcaa
     1261 agttttaaat ctactatcaa ataag
//
LOCUS       DACCWK010000310         1149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.238, whole genome shotgun sequence.
ACCESSION   DACCWK010000310 DACCWK010000000
VERSION     DACCWK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.238"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            12..77
                     /locus_tag="IVW65_002883"
     CDS             12..77
                     /locus_tag="IVW65_002883"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7901694.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            202..939
                     /gene="erm(B)"
                     /locus_tag="IVW65_002884"
     CDS             202..939
                     /gene="erm(B)"
                     /locus_tag="IVW65_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7901695.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          200 c          185 g          343 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc gaaacagact aaaaacagtg
       61 attacgcaga taaataaata cgttagatta attcctacca gtgactaatc ttatgacttt
      121 ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt ttatagatgt
      181 aatcacttca ggagagatta catgaacaaa aatataaaat attctcaaaa ctttttaacg
      241 agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt
      301 tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag
      361 gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg
      421 aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag
      481 aggtataaaa ttgttgggaa tattccttac catttaagca cacaaattat taaaaaagtg
      541 gtttttgaaa gccgtgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt
      601 accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa
      661 ttgcttaagc tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata
      721 aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt
      781 gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa
      841 gcaatgaaac acgccaaagt aaacaattta agtaccatta cttatgagca agtattgtct
      901 atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc gcttttttaa
      961 atttggaaag ttacacgtta ctaaagggaa tggagataaa ttattagata tactactgac
     1021 agcttccaag aagctaaaga ggtccctagc gcctacgggg aatttgtatc gataaggggt
     1081 acaaattccc actaagcgct cgggacccct tgtaggaaaa tgtcctaagt gtggcaacaa
     1141 tattgtatt
//
LOCUS       DACCWK010000311         1122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16071
            SAMN14604028-rid9902973.guided.240, whole genome shotgun sequence.
ACCESSION   DACCWK010000311 DACCWK010000000
VERSION     DACCWK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604028
            Sequence Read Archive: SRR11551411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744147.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/23/2020 23:41:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,823
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 35 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 8 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604028-rid9902973.guided.240"
                     /isolate="UW16071"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: North Rhine Westphalia"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            406..954
                     /gene="aac(6')"
                     /locus_tag="IVW65_002885"
     CDS             406..954
                     /gene="aac(6')"
                     /locus_tag="IVW65_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7901696.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          399 a          165 c          219 g          339 t
ORIGIN      
        1 tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct
       61 tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag
      121 attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa
      181 gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat
      241 ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag taagataaat
      301 aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt
      361 tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa
      421 tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag actgacttgg
      481 ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga
      541 atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa
      601 tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac
      661 caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc
      721 acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg
      781 tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa
      841 ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac
      901 aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa
      961 gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga aaattacttc
     1021 ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa caccgtgtac
     1081 cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aa
//
